LOCUS       DAOSNN010000001        35638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_1229_length_35638_cov_4.917629, whole genome shotgun sequence.
ACCESSION   DAOSNN010000001 DAOSNN010000000
VERSION     DAOSNN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 35638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35638
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1229_length_35638_cov_4.917629"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLQ70_00005"
     CDS             <1..333
                     /locus_tag="PLQ70_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33027.1"
                     /translation="NDAETFIPIQQMLNEKVKLTFNDYPEQAGNFGIYNQNERLQNIS
                     FNYNRTESDLAQANEEAISDYKTIDSIETVFNTLQTDRTDNQIWKWFVIFALLFLVTE
                     MAIIRFMK"
     gene            447..1697
                     /locus_tag="PLQ70_00010"
     CDS             447..1697
                     /locus_tag="PLQ70_00010"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQE33028.1"
                     /translation="MSTIIREAKIIDSESPFHNQTVDILIVDGIIQKIGTSLPNSDNA
                     EEVKLNNLHISQGWFDSSVSLGEPGFEDRETIANGLNVAAKSGFTAIALQPNSYPIID
                     NQSQINFVKSKANGFATALFPIGALTKGSEGKDMAELFDMKNAGAVAFGDYNKSLENA
                     NLLKIALQYVQDFDGLVIAFAQDSNIKGNGVANEGVVSTRLGLKGIPNLAEELQIARN
                     LFLLEYTGGKLHIPTISTAKSVQLIKEAKAKGLQVTCSVAVHHLVLTDEKLENFDTRC
                     KVSPPLRTEADRKALLAGILDNTIDLITTDHNPIDIEHKKMEFDGAKNGTIGLESAFG
                     TLLTVLPLEKVIEKLTSGKTTFGIEKQSIAEGKKANISLFNPEEKGIFTNENILSKSK
                     NSAFLGTEIKGKAYGIYNQGKLVL"
     gene            1750..2079
                     /locus_tag="PLQ70_00015"
     CDS             1750..2079
                     /locus_tag="PLQ70_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33029.1"
                     /translation="MENNNIEEGKNIAIVSYITVIGAIVAIFMNQDKKNSFAAFHIRQ
                     ALGIFISFFLIGYFIGYFDSWMISSAFYIFYFILWVYGFLGALQGQMKLIPLLGEQFQ
                     KIFKNIE"
     gene            2083..2727
                     /locus_tag="PLQ70_00020"
     CDS             2083..2727
                     /locus_tag="PLQ70_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQE33030.1"
                     /translation="MNFSLEYLVREPKIKLDKNPLLLLIHGYGSNEQDLFSFATELPN
                     EYYIVSARAPYDIQYGSYAWYAINFDADQNKFSDNEQARTSRDLIAQFIDELEANYSI
                     DSNNVTLIGFSQGAILSYAVALSYPEKVQRVVAMSGYINPEIIEENYLKNSFSNLKIF
                     TSHGTVDQVIPVEWGRKAKPFLENLGINTTYKEYPIGHGVSPQEFNDFKNWLEK"
     gene            complement(2800..3300)
                     /locus_tag="PLQ70_00025"
     CDS             complement(2800..3300)
                     /locus_tag="PLQ70_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33031.1"
                     /translation="MKKSLLLYLFIILLLLNVFTYMYFSKELAFEKNKTAKMESKLKK
                     DVDIANAKLVDANYFSLEKNENAQNYFNPDNATKTIQVEKLIPVVTEKLMDFNSNPKG
                     NPYIGQDQIGANKFIINKVKILNHRWIIADFSDGEYWGEVLIKYFVNDDETVSFETFQ
                     SLLYQK"
     gene            complement(3313..4077)
                     /locus_tag="PLQ70_00030"
     CDS             complement(3313..4077)
                     /locus_tag="PLQ70_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQE33032.1"
                     /translation="MNVYFLGTGTSQGIPVIGSNHSVCQSTDLKDKRLRVSVWIHWEN
                     HSYVIDCGPDFRQQMLVSNCQKLDGILYTHEHSDHTAGLDDIRPFFFKQGDIPIYAHQ
                     RVLANLKLRFDYIFQIENKYPGAPSVHPIEVVNNHPFQIGNKRAIPINVMHGKLPVFG
                     YRIDDFAYLTDVKTIDESEISKLKNLKVLVVNALREEVHHSHFNLSEALDFINLIQPE
                     KTYLTHISHIMGFHEQVQQKLPKNVYLAYDNLEITI"
     gene            4176..5639
                     /locus_tag="PLQ70_00035"
     CDS             4176..5639
                     /locus_tag="PLQ70_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE33033.1"
                     /translation="MKRDTEIKLKGDKVIEQIPSLKDKALRINLNENIYGTFSEIGAG
                     QETVRHFFRAGGSSGTIAKAMSAYDKDFSDAIYGVEEDGRYVTESRLKKILTHEAGLI
                     EKRLSRKKHPNKIFFSYANTVATIDFAKQFKGHGWVGIKYQLEPEEEYNEIILHIRFK
                     ETDVRLQQETLGILGVNLIYGAFYKYNNPKHLLRYLYDHLDKDQLEIDTINFSGPRFA
                     NVDNRLMSLQLVKNGMTDAVIFDPEGKNILPAAILYKKNILAIRGSFRPVTKVNMDMY
                     EESLKMFLNEQKVNPDNTLVIFEITLSNLRSDGEIDEKDFMDRAQLLCSLGQTVMISN
                     FQEFYKVVEYFAKYSKARMGLAMGVNNLVEIFDEKYYRHLSGGILEAFGKLFYRDLKV
                     YLYPMIDENGEILNSETLKIHPRMKELYKFFKYNGKVVDIENYNPKNLVVFSREVLKM
                     IGESKLGWESMLPEGVAKIIKKKKLFGYDPNMLLEKK"
     gene            complement(5704..6156)
                     /gene="bcp"
                     /locus_tag="PLQ70_00040"
     CDS             complement(5704..6156)
                     /gene="bcp"
                     /locus_tag="PLQ70_00040"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HQE33034.1"
                     /translation="MTTLKKGDQAPAFFGVDQDGKPHQLEDYKGKKLVVFFYPKASTP
                     GCTAEACDLRDNFERFKANNYALLGVSADSAKAQAKFKDKYDLPFPLLADEEKSVIED
                     FGVWGPKKFMGREYDGIHRTTFVIDENGIIEEVISDVKTKAHAAQILK"
     gene            6224..6883
                     /gene="nth"
                     /locus_tag="PLQ70_00045"
     CDS             6224..6883
                     /gene="nth"
                     /locus_tag="PLQ70_00045"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQE33035.1"
                     /translation="MTKQERVTFVINTLKELYPEIPIPLDHKDPYTLLVAVLLSAQCT
                     DVRVNQITPLLFAKADNPYDMVKMSIEEIKEIIRPCGLSPMKSKGIHGLSQILIEKHG
                     GEVPQSFEYLEELPAVGHKTASVVMSQAFGVPAFPVDTHIHRLMYRWNLSDGKNVVQT
                     EKDAKRIFPEETWNDLHLQMIWYGREYSPARGWDIEKDIITRTIGKKSVLEEYHKKTS
                     R"
     gene            complement(6899..7042)
                     /locus_tag="PLQ70_00050"
     CDS             complement(6899..7042)
                     /locus_tag="PLQ70_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33036.1"
                     /translation="MAKIYSKKELVTPKMKPKKETISFLLNYSKALNVIKVNDKSFEI
                     IAN"
     gene            complement(7095..7679)
                     /locus_tag="PLQ70_00055"
     CDS             complement(7095..7679)
                     /locus_tag="PLQ70_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQE33037.1"
                     /translation="MASIELPDALLVTKYIQGDENALTVLINRHQSKIFGFIYSKIAD
                     RDISNDIFQDTFIKVIRTLKSNSYNEEGKFLPWVMRIAHNLIIDHFRKNKKMPMFRET
                     EEFSIFSIMSDDSLTIENQIIQDQVEVDLKKLIEELPADQKEVLVMRMYQNMSFKEIS
                     ETTGVSINTALGRMRYALMNMRKVIDKHQIILTN"
     gene            7987..10776
                     /gene="uvrA"
                     /locus_tag="PLQ70_00060"
     CDS             7987..10776
                     /gene="uvrA"
                     /locus_tag="PLQ70_00060"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQE33038.1"
                     /translation="MPIDLSTLDPKQNIIIKGAQVHNLKNVDVAIPRNKLVVITGLSG
                     SGKSSLAFDTLYAEGQRRYVESLSSYARQFLGRLDKPKVEYIKGIAPAIAIEQKVNTT
                     NARSTVGTSTEIYDYMKLLFARIGRTFSPISGQEVKKNTVSDVIKDVKEFGTDSKWLL
                     LAPIHLEEGRALEDKLKALLNQGFSRILVNNETARLDDLESHSLDNKDILLIIDRIVV
                     KDEEEFYNRLADAVQTAFYEGKGICYLQELNTHKRFSYSNNFELDGISFLEPNVHLFS
                     FNNPYGACPVCEGYGNIIGIDEELVIPNTALSIFENAIFPWRGESMSWYRDELVNKAY
                     KFDFPIHKPFFELSEAQKELVWTGNSYFQGLNDFFKELEEKNYKIQNRVMLSRYRGKT
                     KCNSCKGKRLRIEASYVKINSKTVSDLVDLPIKHLVTFFKNIDLDVYEQQIAKRLLVE
                     INNRLSFLTEVGLDYLTLNRNSATLSGGESQRINLATSLGSSLVGSMYILDEPSIGLH
                     PKDTERLIKVLLSLRDLGNTVIVVEHDEDIMKAADMIIDIGPEAGTFGGELVAQGTFN
                     EILKSTSLTAKYLNGELEIKTPRIRRKWSNYIEILGARENNLQNIDVKFPLDVLTVIT
                     GVSGSGKSTLVKKILFPAMQKKLDGATTEKAGQFTEMRGYYHKIQHIEYVDQNPIGRS
                     SRSNPVTYIKAYDDIRDLFAKEKLSKIRGYQAKHFSFNVDGGRCETCKGEGTINVEMV
                     FMADVQLHCETCNGKRFKKEVLEVAFDGKNIHDILTMTVDDSIAFFTANKQTKIIQKL
                     QPLQDVGLGYVQLGQSSSTLSGGEAQRIKLASFLVKGATKEKALFVFDEPTTGLHFHD
                     IKKLLASFDALIDKGHSILVIEHNLDLIKCADWIIDLGPEGGENGGLLLAEGTPEEIL
                     KIKKSVTAKYLKEKL"
     gene            10821..13652
                     /gene="uvrA"
                     /locus_tag="PLQ70_00065"
     CDS             10821..13652
                     /gene="uvrA"
                     /locus_tag="PLQ70_00065"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQE33039.1"
                     /translation="MLENDNNIEVLGARVHNLKNIDVKIPREKLVVITGLSGSGKSSL
                     AFDTIYAEGQRRYVETFSAYARQFLGGLERPDVDKIDGLSPVIAIEQKTTSKSPRSTV
                     GTITEIYDFLRLLYARAADAYSYNTGEKMVSYDDDQIKELITKDFAGKRINILAPIIR
                     ARKGHYAELFQQIAKQGFLKVRINGEIKDITTGMKLDRYKTHDIEIVIDRMVIEDTPD
                     NEKRLTESIKTAMYQGENVLMILDQDTNEIRFFSRNLMCPSTGISYQNPEPNLFSFNS
                     PKGACDHCKGLGTVNEINLKKIIPNPKLSIKAGGFVPLGEYKSSWIFKQLEIIGEKFG
                     FKLTDAIETISEEAMEMILNGGKEKFSVESKVLGVTKEYKIEFEGISHFIKNQYDESG
                     SATIKRWAKEFMDEVKCPVCEGSRLKKEALFFKINEKNIAELCDMDISDVTAWFLELN
                     THLSEKQKTIATEVIKEIKDRLNFLMNVGLDYLALSRSSKSLSGGEAQRIRLATQIGS
                     QLVGVLYILDEPSIGLHQRDNAKLIHSLEQLRDIGNSVIVVEHDKDMIEQADYVIDIG
                     PKAGKYGGEIISIGTPAETLKSDTITAQYLNGKMKLEIPKKRREGNGNFLKLTGATGN
                     NLKNVSIDLPLGKMICITGVSGSGKSTLINETLYPILNAYYFNGVKKPQPYKKIEGLE
                     HIDKVIDIDQSPIGRTPRSNPATYTEVFSEIRNLFTMTSESMIRGYKAGRFSFNVKGG
                     RCETCEGSGVRTIEMNFLPDVYVECETCQGKRFNRETLEIRYKGKSISDVLNMTIDEA
                     VPFFENIPKIYRKVKTIQDVGLGYITLGQQSTTLSGGEAQRIKLAGELSKKDTGNTFY
                     ILDEPTTGLHFEDIRVLMEVINKLVDKGNTILIIEHNMDVIKLADYIIDIGPEGGKGG
                     GEVVAKGTPEEIAKNKKSYTAKFLKKELK"
     gene            13813..14703
                     /locus_tag="PLQ70_00070"
     CDS             13813..14703
                     /locus_tag="PLQ70_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQE33040.1"
                     /translation="MKKTKSVLFLAFLLQSILVLGQELPKEMPLFPNGIKDNPITHEQ
                     KETFADSLVKKGSLSQKDRVFRNISEPTYMLFPAGESNNKHVAVIILPGGGFHTNWLD
                     KEGTDLALWLSAQGISCMVVKYRTNSKDEKGKYIIPMETYFDDALKIDINASVMTMMG
                     MAEKLKFDKDKIGVLGFSAGGFMAEYLTFTSVSKNLPWKPAFTGLIYAGDRLKLLESV
                     KDKSALPPFFMAIARDDKKWKLQRALPFLSAVVEQVDKSELHVYSKGDHGFGLAYDEG
                     HSVGMWKDSFYNWILDIYNK"
     gene            complement(14880..15470)
                     /locus_tag="PLQ70_00075"
     CDS             complement(14880..15470)
                     /locus_tag="PLQ70_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQE33041.1"
                     /translation="MKNFIVYLGITLMAFTSVSLASNTNSIGNQKKLNVFSETPSTLA
                     IAISKGEIETVKQLIESGTKVNKKLNGMTPLMYAARYNKVEIIKYLLQKGADRNIEDS
                     QGFTALNYAELSNAYEAIAVLKSLPVYNQATLSMDYDTAFMDSDSVLFVQSKKTIDEI
                     IAEDEKIIESTGFDDIQPLDFKMINEKSVLDNHINS"
     gene            16098..16826
                     /locus_tag="PLQ70_00080"
     CDS             16098..16826
                     /locus_tag="PLQ70_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQE33042.1"
                     /translation="MRLEDFDNDEDKVIQNKFKRKTWNEIRTNDSWGIFKIMSEFVNG
                     YETMGRIGPCVTIFGSARIKPEDKYYQLAEKIAYKISKAGYGIITGGGPGIMEAGNKG
                     AHLGGGTSVGLNIELPFEQHFNPYIDHDKNLNFDYFFVRKVMFVKYSQGFVVMPGGFG
                     TLDEMFEALTLIQTKKIGKFPIILVGTTYWSGLIEWMKKVLVEEEKTINATDLDLFKI
                     VDTEDEALAVLDKFYKKYDLSPNF"
     gene            16967..19696
                     /locus_tag="PLQ70_00085"
     CDS             16967..19696
                     /locus_tag="PLQ70_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQE33043.1"
                     /translation="MEHKTLTVLQEIEFFNQSEDTLTSIVLNDWNNAYSSKTTPLAKR
                     FSDEFYRGFHLASEKERGNTTNLNIATADKKNLFCERTEKNPDIIIVQLDKKLAPNQK
                     IVLHFTYIIKVPSDKFTHYGYTERGGMYLRNWFLTPARYENHSFVKNSNLNLDDIANA
                     VSNFDILFRIPKNIELTTDLDEEKTTPIENFSIHKLNGKNRTDFSIFLEPKTSFRSFK
                     NNLVEVETNLRGYKADEIQKAIAIDRVVNFTNTLIGESHTEKITVSQADYDRNPFYGL
                     NQMPAFMVPFPDEFLFEIKFLKTYLNNYLKNNLKLDPRKDNWIFDGIQVYAMMEYMKE
                     NYPDCKMTGNISNYKLFKGYHLLNLDFNEQFSYFYLLMARKNLDQPLGAPKNTLIKFN
                     EQIASKYRAGLSFIYLDNYLGNGSVANSIQQFNNRNAIGQTTENDFETLLKSVTNKDI
                     NWFFKTIIHSRDIIDYKFSDVSKSKDSISFSLKNRTGVAVPMPIYGLKNDTIVFKKWL
                     DPQKNDSIYTFERHQANKIVINYKNEVPEFNLRNNWKKLEGLFPNNRPIKFALIKDLE
                     DPFYNQVIYAPILTYNVYDGFSPGMRFHNRAILNRPFLYDINPTYALKSKTLTGSAIF
                     MINKDYRDSNLFNVRYSISGNYFHYAPDATYLKINPMMLMQIRNENYRDNRKQLLLFR
                     QVIINRQQSDIVVDSSLQDYSVFDAKYVNSRTELTNHISFMGDVQFSGKFGKISSEVQ
                     YRKLYEDNRQLNLRLYTGVFTYNKSNSDFYSFALDRPTDYLFDYAYLGRSSSTGIVSQ
                     EFILAEGGFKSKLNTPYANQWMTTLNGSYSIWNWIEAYGDIGLVKNSQQKEKFCYDGG
                     IRLNLLTDYFELFFPIYSNKGWEISQPNYNEKIRFVITLNPDKFIQLFTRKWF"
     gene            19891..22299
                     /locus_tag="PLQ70_00090"
     CDS             19891..22299
                     /locus_tag="PLQ70_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQE33044.1"
                     /translation="MIKEKANSTLTFEDFKTEVLKDYKIAITSRECSLLGRKEVLTGK
                     AKFGIFGDGKEVPQLAMAKFFQNGDFRSGYYRDQTFMMAIDQFTPQQFFAGLYGHTDI
                     SQEPMSAGRQMGGHFVTHSLNEDGSWKDLTQQKNSSADISPTAAQMPRMLGLAQASNI
                     YRSIPELANKSNFSNGGNEISWGTIGNASTSEGIFFETINAAGVLQVPIILSVWDDDY
                     GISVHAKHQTTKESISEILKGYQRDENNNGFEILKVQGWDYPSLIETYQKASDLAREH
                     HVPVLIHVTQLTQPQGHSSSGSHERYKDENRLNWEKEFDCIRQMKLWMIAINIASPEE
                     LEEIDQIIRKEVLEAKKTAWSAFINPIIEEQQELVQLLEKIGPTSKNSDRILKHKTNL
                     SNIKAPLKKEILSTARKVLRLIINEESKEELSNWITAYFEKNQPKFSKSLFSESNLNV
                     FSVKEVLPQYEENAKEDTDGRMILRDNFNELFTKYPEVLIFGEDAGSIGDVNQGLVGL
                     QEKFGETRVADVGIREASIIGQGIGMALRGLRPIAEIQYLDYLLYGVQILSDDLATLQ
                     YRTLGKQKAPLIIRTRGHRLEGIWHSGSPMGMLINALRGIHILVPRNMTKAAGFYNSL
                     LECDEPALVIECLNGYRLKEKMPLNYGEFKTPIGVVETLKEGKDITLVSYGSTLRLVE
                     AAAKELMEVGIDCEIIDIQSLLPFDVHHDIVKSITKTNRLLIIDEDVPGGASAYILQE
                     IIETQNGYSYLDSKPQTLTAKPHRPAYGTDGDYFSKPSIEDVFEKVYAMMNEVNPSKY
                     PALY"
     gene            complement(22392..23861)
                     /locus_tag="PLQ70_00095"
     CDS             complement(22392..23861)
                     /locus_tag="PLQ70_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQE33045.1"
                     /translation="MGIVLNQSFKNTIITYIGFAIGGINTIYLYPVFLGATYYGLTNY
                     ITSSANVIMPLFAIGMQNTLVKFYSQYETEAERNRFLSFTVLFPLLLIIPLLFIGFLF
                     QDEILFFLSKKNAVVKNYIWLIPFIGLCMAYFEIFYAWLRVHMHSVFGNFIKEVGLRT
                     ASLFLLMGVYFHWITVEGFVYATAVVYLIALLVTMFYAFSIQKPNFQFTIPVNTKDIL
                     TYTFYIILSGSVANLLLDGDKMILNQYMNIENIAFYSVATYIALVISVPSRAMHQIVY
                     PITAKLMHENKHDEMNILYKKTSINLQMVGGYVMLGIFVNINMLYKMVPPEYAGGIMV
                     VFMIGLSKYFDLILGNNNAIIFNTKYYRTVLFLGVLLVILTVGLNMIFIPLYGIIGSA
                     FATLLSITMYSLAKLLFVVKKLDLYPFTKQTLYSLALTFVLFVAFYIWDFPFNPIISI
                     ILKSILLTIAYVYLNYRFVISTEINEVMDKYLKKFKIIK"
     gene            complement(23870..25162)
                     /locus_tag="PLQ70_00100"
     CDS             complement(23870..25162)
                     /locus_tag="PLQ70_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQE33046.1"
                     /translation="MKPEKLLIITYYWPPAGGPGVQRWLKFVKYLPDFGVQPIVYIPE
                     NPTYPIVDENLVKEVSDKAVVVKHKIFEPYQLASFLSKNKTKKISSGIIPNQKKQSFL
                     DKLFLWIRGNLFIPDARVYWVKPSVRFLEKYIVENNIDTIVTSGPPHSLHLIGLELKQ
                     KLNVKWFADFRDPWTTIGYHKSLRLSNFAAKKHKKLEHQVLNTADTIIVTSNTTKTEF
                     QAITTKQIAVITNGYDTENVQKQPLDAKFSLAHIGSFLSERNPQLLWETLVELLDEIP
                     DFKSHLEIKLIGAVSQEVLETISNFGLNSYLNNLGYVSHSEAIAHQRKSQVLLLIEIN
                     SEDTKSIIPGKLFEYMVSNRPIIAIGPNGSDFAEIITQTNTGVFFDYSEKMKLKSVLL
                     GYYNQFLEGKLQSNAVGLQQYSRKSLTEKLVQLLTSNF"
     gene            complement(25165..27597)
                     /locus_tag="PLQ70_00105"
     CDS             complement(25165..27597)
                     /locus_tag="PLQ70_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HQE33047.1"
                     /translation="MKQLQKLYPHAIVLLGFVLISLIYFYPVLQGKQILQSDIAQYTG
                     MAKEQNDFRASNHQEPYWTNSAFGGMPTYQLGAKYPHDYIGMIDDVLRFLPRPADYLF
                     LYFIGFYVLLLVLKTDPLKAFFGALAFGFSTYLIIILGVGHNAKAHAIAYMPMVIAGF
                     IMVFQKKHIWGGLLTMFAVALEINANHFQMTYYLLIFLLVLSGYFIYLAIKEKEFKPL
                     LTSFGVLAIAGIFALGANATNLLATAEYAKFSTRGKSELTFTPDGKKAETNSALSHDY
                     ITEYSYGIAESFNLIAPRLFGGSNSEALGTDSEMYKFMTSQGVPESQANEFVSAMPTY
                     WGDQPIVAAPAYIGVVVFFLGILALFADKRKIKYVFLSGAIVALILSWGKNFPTLTDF
                     FIDNVPMYDKFRAVSSIQVILELCFPVLAIMGLQSFFTLEKEARFKTLWQSAAVGFGI
                     IVGLFLLKGSFSFAGANDGYYIQNYGPSFIDALKADRKTLYSADLLRSGFFLLLVAAA
                     LWLFIKEKLAQNTTIIAVGLLMILDLVLVDKKYVDAEGFVSKREVEVPFQETPADVQI
                     LQDKSHYRVFEIDGNLSSARASFFHKSIGGYSAVKPRRMQQLFDYQIAKNNIEILNML
                     NVKYIIQTDSTGNSVPVQNPEANGNAWFIKDLLLVNKPDDVMKALGKLDTKHDAVFNV
                     HDYGKKYKNARLKEDFDDSGTIQLDVYKPNYLKYTSNNSGKGLAVFSEIYYENGWNAY
                     IDGEKKEHFPVNYVLRAMRVPEGKHTIEFKFEPQVIQTGSTITLFCFIGMLLLLIGGI
                     YVERKKSLNLKP"
     gene            complement(27645..27926)
                     /locus_tag="PLQ70_00110"
     CDS             complement(27645..27926)
                     /locus_tag="PLQ70_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HQE33048.1"
                     /translation="MQEASFQGFIRTLIYIIAIYYIIKFLARLFLPLLVKKVVEKAGE
                     NFQKQQQYAQGNPWQKTPNNDEIIIDTANAKRTRETKKVGDYVDYEEID"
     gene            28050..29009
                     /locus_tag="PLQ70_00115"
     CDS             28050..29009
                     /locus_tag="PLQ70_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQE33049.1"
                     /translation="MYKIKNLVLAAFLMFPLIHYGQHTDQINSNRPGETMSAFAVGKS
                     VIQVESGIYGIQENHSILNYDANGFGIDMTLRWGLFMEKLELIADLQYQYEYLNPAIA
                     YPEKSAMKQTILGAKYLIYDPFKNYEKNINVYSWKANHSFNWHQFIPAVSVFAGANIT
                     LNDNPYYFSPKAAISPKIALITQNHFGDGTWVFVTNIIADYIGTDYPSYGYGITLTKG
                     FNRKWSGFIENQGYMSDFYSDAIVRGGAAYLIHDDLQIDASISTSLKDTPSILYGGIG
                     FSWRYDAKYKEVRINIDDDKKTKSQKKVEKKNKKRKDQIETTS"
     gene            29018..30136
                     /locus_tag="PLQ70_00120"
     CDS             29018..30136
                     /locus_tag="PLQ70_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase"
                     /protein_id="HQE33050.1"
                     /translation="MITIIEAVTKKEMTEFVKFPFSLYKDNKYWVPPIIADELESFDR
                     TKNPAFESAEAYFYLAYKDNQIVGRIAAIINWGEVNNQHKKKVRFGWFDVIDDIEVTK
                     ALLEKVYELGRKNNLEHVEGPMGFSNLDKVGVLTEGFDEMGTMITWYNHPYFASHLEQ
                     LGFVKEKEYIESIFPFSNVKPEFFLKAQALIKKRYELRSLNFTKTKDIMPYVDKMFDL
                     FNNSYASLSSFVAITDIQKEYFKKKYISFINPEYIKFVMDKNDNIVAFSIVMPSFSEA
                     LQKAKGKLFPFGFYHLLNARKNSKDVVFYLIGVDPEYQNKGVTAIIFDEYFKTFSEKG
                     IINCIRTPELEENHAIHNLWKNFDPRIHCRRKTFIKNL"
     gene            30297..32288
                     /gene="uvrB"
                     /locus_tag="PLQ70_00125"
     CDS             30297..32288
                     /gene="uvrB"
                     /locus_tag="PLQ70_00125"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQE33051.1"
                     /translation="MKFQVISDYQPKGDQPQAIAKLAQGIEADERYQTLLGVTGSGKT
                     FTVANVIQEVQKPTLILAHNKTLAAQLYSEFKQFFPNNAVEYFVSYYDYYQPEAFMPV
                     SGVFIEKDLSINEELEKMRLSTTSSLLSGRRDIIVVASVSCIYGIGNPVEFQKNVIAI
                     EKDQMISRTKLLHSLVQSLYSRTEADFNPGNFRIKGDTLEVYPSYADDAYRIHFFGDE
                     IEEIESFDLKTSKVIERFEKLTIYPANMFVTSPDVLQNAIWEIQQDLVKQVDYFKEIG
                     KHLEAKRLEERTNFDLEMIRELGYCSGIENYSRYLDGRDAGSRPFCLLDYFPKDYLMV
                     VDESHVTLSQVQAMYGGDRSRKENLVEYGFRLPAAMDNRPLKFEEFEAMQNQVIYVSA
                     TPADYELQKCDGVYVEQIIRPTGLLDPIIEIRPSLNQIDDLIEEIQTRAEIDERVLVT
                     TLTKRMAEELAKYLDKVSIRCRYVHSDVDTLERIEIMQDLRKGLFDVLIGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSHRSLTQTIGRAARNLNGKAIMYADKITASMQKTIDET
                     NYRRTKQINFNTENNVIPMALNKKIESAFTNNKLVEYELGHIPSLAAEPETAYLSKPE
                     IEKLIREKRKAMEKAAKDLDFMQAAKLRDAIKALQEQLS"
     gene            complement(32352..32753)
                     /locus_tag="PLQ70_00130"
     CDS             complement(32352..32753)
                     /locus_tag="PLQ70_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HQE33052.1"
                     /translation="MPTYEEKISLLLEMIAFATIDGSLHKKEYTFLSIVADELKITKE
                     VFDDLFHQELPFIVIKSEFERFHQFYRLALLMHCDGVLHPKEEVAIQQIGIDMGLNPI
                     VTNRILKMMKKTQSPVIEAEVLLEIFKEQHN"
     gene            32810..33616
                     /locus_tag="PLQ70_00135"
     CDS             32810..33616
                     /locus_tag="PLQ70_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQE33053.1"
                     /translation="MIQRFTVVIIFFIAIGTTFAQQSTASKQVSTFTIEAPQLHTTKK
                     IWIYLPKNYKSSTKKYPVIYMHDAQNLFDAKTSFVGEWNVDEKLDSLNAQVIVVGIEH
                     GNDKRMEELTPYKNAKYGGGNADNYLDFIVKNLKPAIDTKYRTKTNARNTAIMGSSLG
                     GLVSYYAILKYPEVFGKAGLFSPSFWFNRKEIIELTNSIPKLKTKIYFLCGDNEGDAD
                     MINDLNKIDELISEKRCECVQLTKKKIVKGGQHNEKLWRDGFAKAYVWLF"
     gene            33650..34978
                     /locus_tag="PLQ70_00140"
     CDS             33650..34978
                     /locus_tag="PLQ70_00140"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HQE33054.1"
                     /translation="MVKIQIKNRVSTLLILFLVSTATFAQKIPERKIKKLIEKSEILN
                     EHFTGFALFDQDQDKMIYEQNASKHFVPASNTKIYTFYTVLNMLGDSIPALKYVTKGD
                     SLIFWGTGDPTLTYSTFKSTKVLDFLKKSEKKLYFASHNYKGNFYGYGWNYDDYLEDF
                     QPELTAFPVDGNTAKVYKTEKGEVTVKPPFLQPYFKADPNYIPENFLIHRDFYTNQFT
                     YPTTIAKTGYLQEIPFKTSPELTISLLEEKINKEISLLNITLPQDFKIIYSEKTDDVL
                     RKMMLVSDNFIAEQLLLVCSSTLSNDLSTKVIIDYSKTNFMKEFANDIVWVDGSGLSR
                     YNLFTPNTSIILLKKISLTINDEERLHSLFPAGGVSGTLKRAYKTDADKPFVWAKTGT
                     LTNVYNQSGYIITRKGKKLIYSLMNNNFTRTTEEIRDEMARIITKIHNDY"
     gene            35051..35509
                     /locus_tag="PLQ70_00145"
     CDS             35051..35509
                     /locus_tag="PLQ70_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HQE33055.1"
                     /translation="MTNNDIFKKLRVALMLRDDQIVEILELVDFRISKTELSAFFRAE
                     NHENYVECGDQVLRNFLNGLVIHLRGTKDNPKNPNDVLAKHKAQIPKKEGNSDRPEFK
                     AKPKDEERVRGDQSPSKTKPAAKKKPFKKPAPKVQVVEKVKYNFGKNKKS"
BASE COUNT        12515 a         6017 c         6161 g        10945 t
ORIGIN      
        1 aacgatgctg aaactttcat tccgatacaa caaatgctga atgaaaaagt aaaattgacc
       61 tttaacgatt atcctgaaca agccggtaat tttggaattt acaaccaaaa tgaacgtttg
      121 caaaacatca gttttaacta caacagaacc gaaagtgatt tggctcaagc caatgaagag
      181 gcgatttctg attataaaac aatcgattct atcgaaaccg tttttaacac cttacaaacc
      241 gaccgaactg acaatcaaat ttggaaatgg tttgttatat ttgcattgct ttttctggta
      301 accgaaatgg cgattatacg attcatgaag taaaaaagct agatacaatt ttaccgcaaa
      361 ttcgcaaatt attctaactg taagtaattg aaatttgcga atttgcggta aaaataaaaa
      421 aacattcaaa taattaaaca attattatga gcacaatcat ccgggaagcc aaaattatcg
      481 attccgaaag tccttttcac aaccaaaccg ttgatatttt aattgttgat ggcatcatcc
      541 aaaaaattgg aacttcactt cctaattcag ataatgccga agaggtaaaa ctaaacaatt
      601 tacatatttc ccaaggttgg tttgattcta gcgtttcgct tggagaacct ggttttgaag
      661 accgtgaaac tattgccaac ggtttgaatg tagctgcaaa aagtggtttt acagccattg
      721 ctttgcaacc caactcctac ccgattattg ataaccaatc gcaaattaat tttgtaaaaa
      781 gtaaagccaa tggttttgct acagcacttt ttccgattgg cgccctgacc aaaggaagcg
      841 aaggaaaaga tatggcggaa ttatttgata tgaaaaatgc aggtgctgtt gcttttggag
      901 attacaacaa gagtttggag aatgccaact tactgaaaat tgccttgcaa tacgttcagg
      961 attttgatgg tttggttatt gctttcgccc aagattcgaa catcaaaggg aacggagttg
     1021 ccaatgaagg agtagtttct acccgattgg gattgaaagg aattccaaat cttgccgaag
     1081 aattgcaaat cgccagaaac ttgtttctat tggaatatac cggtggaaaa ttacatattc
     1141 cgaccatttc aactgctaaa tcagtacagt taatcaaaga agcgaaagcc aaaggattgc
     1201 aagttacttg tagcgttgca gtgcatcatt tggttttaac cgatgaaaaa ctggaaaact
     1261 ttgatacccg atgcaaagtt tcgccaccat tgcgaacaga ggctgacaga aaagcattgc
     1321 ttgccggaat tttggacaat acgattgacc taattacaac cgaccacaat ccgattgata
     1381 ttgaacataa aaaaatggaa tttgatggtg caaaaaatgg aactattggc ttagaaagtg
     1441 cttttggaac tttattaacc gtattgcctt tagaaaaagt aatcgaaaaa ctgacttccg
     1501 ggaaaactac ttttggtatc gaaaaacaaa gtattgccga aggtaaaaaa gccaatatta
     1561 gcttatttaa tcctgaagaa aaaggcattt ttaccaatga aaatattttg tctaaatcga
     1621 aaaactccgc ttttcttgga acagaaatca aaggaaaagc atacggaatt tacaatcaag
     1681 gaaaattagt tttgtaatta ctagacctca aaagttttgg gaaaaagttt gaggtttaaa
     1741 tcaaaaaata tggaaaacaa caacattgaa gaaggaaaaa acattgcaat tgtgagttac
     1801 ataaccgtta ttggtgcgat tgtagccatt tttatgaacc aagataagaa aaatagtttt
     1861 gctgcttttc atattcgtca agcattgggg atatttattt cgtttttttt aatcggctac
     1921 tttatcggct attttgatag ttggatgatt tcttccgcct tttatatttt ctattttata
     1981 ctgtgggttt atggttttct tggagcgtta caaggtcaaa tgaaactgat tcctttattg
     2041 ggagaacaat ttcaaaaaat atttaaaaat atcgaataat atatgaattt ttccttagaa
     2101 tatctcgtaa gagaacccaa aataaagttg gacaaaaatc cgcttttact cttaattcac
     2161 ggttacggca gcaacgaaca agatttgttt tcattcgcca cagaacttcc taatgaatat
     2221 tatattgttt cagcacgtgc tccatacgac atacaatacg gaagttatgc ttggtatgcc
     2281 atcaactttg atgccgacca aaacaaattt tccgacaacg agcaagccag aacttctaga
     2341 gatttaattg cccaatttat tgatgaattg gaagctaatt attctattga ttccaataat
     2401 gtaaccttaa ttggttttag ccaaggtgct attttaagtt atgctgttgc gctttcatat
     2461 cctgaaaaag tgcaaagagt cgtggcaatg agtggttata tcaacccgga aattatagaa
     2521 gaaaattatc taaaaaactc cttttcgaat cttaaaatat tcacttcaca cggaacggtt
     2581 gaccaagtaa ttcctgtgga atggggacga aaagctaaac cttttttgga gaatttagga
     2641 attaatacta catataaaga atacccaatt ggtcacggcg tttccccaca ggagtttaat
     2701 gatttcaaga attggcttga gaaataaaaa ttaccacaga ttcgcagatt tttatttctg
     2761 atttaaattg aaatctgcga atctgtggta aaaaaatatt tatttttgat acaataaaga
     2821 ctgaaacgtc tcaaaagaaa ccgtttcatc atcattaaca aaatatttta tcaacacttc
     2881 gccccaatat tctccgtcgc tgaagtcggc aataatccaa cggtggttca atattttcac
     2941 cttattgatg atgaatttat tggctccaat ttgatcttga ccgatgtaag gattcccttt
     3001 gggattggaa ttaaaatcca ttaatttttc ggttacaaca ggaatcagtt tttcaacttg
     3061 aatcgttttt gtggcattat cgggattgaa atagttttgg gcattttcat ttttttctaa
     3121 cgaaaaataa ttggcatcaa ccagtttggc atttgcaatg tcaacatcct tctttaattt
     3181 tgattccatt ttggccgttt tatttttttc gaaagccaat tctttactga aatacatata
     3241 ggtaaaaaca tttagcaata gaagaataat aaaaaggtac agtaataatg attttttcat
     3301 ttcaagataa tattaaatag taatttctaa attatcgtaa gctaaataaa cgtttttagg
     3361 cagcttttgt tgtacttgtt cgtgaaaccc cataatatgg cttatatgag tgagataagt
     3421 cttttcgggt tgaattaaat ttataaaatc caaggcttca ctcaaattaa aatggctatg
     3481 atgtacctct tctctcaaag catttacaac caaaactttc aaatttttaa gttttgaaat
     3541 ttcactttca tcaatcgttt ttacatctgt caaataagca aaatcgtcaa ttcggtaacc
     3601 aaaaacggga agttttccgt gcataacatt tattggaatc gcccttttat ttcctatttg
     3661 aaaaggatga ttatttacaa cttcaatggg gtgaaccgaa ggtgctcccg gatatttatt
     3721 ttcgatttga aaaatataat caaatcgaag tttcaaatta gcaagaacgc gctgatgtgc
     3781 ataaattggg atgtcgcctt gtttgaaaaa aaaagggcga atatcatcca atcctgcggt
     3841 atgatcagaa tgttcgtgag tataaagaat tccatccagt ttttgacaat tcgaaactag
     3901 catttgttgt ctaaaatctg gcccacaatc aattacatag gaatgattct cccaatgaat
     3961 ccaaaccgaa acccgaagtc ttttatcttt taaatctgta ctttgacaaa ctgaatgatt
     4021 actaccaata actggaattc cttgagaagt acctgtccct aaaaaataaa cattcattta
     4081 atataacatt tgttaataat tgtacaaaaa tagcaataaa tctcttcatt tagaaacatt
     4141 ttttattaac tttgcaccaa aacagcgatt tctttatgaa aagagacacc gaaattaaat
     4201 tgaaaggtga caaagtcatc gaacaaattc cttctttaaa ggacaaagcg cttcgtatca
     4261 atttgaatga aaatatatac ggaacttttt ctgaaattgg tgctgggcaa gaaaccgtaa
     4321 ggcatttctt tagagctgga ggatcgtcag gaacaattgc aaaagcaatg tcagcctacg
     4381 acaaagattt tagcgatgca atttacgggg tcgaagaaga cggacgctat gttacagaaa
     4441 gccgtctcaa aaaaattctg acccacgaag ctggtttaat cgaaaaacga ttgagcagga
     4501 aaaagcatcc caataaaata tttttcagtt atgccaatac cgttgctacc attgactttg
     4561 cgaagcaatt caaaggacat ggctgggttg gaataaaata tcaattagag cctgaagaag
     4621 aatataatga gatcattcta cacattcgat ttaaagaaac cgatgttcgt ttgcaacaag
     4681 aaacccttgg tattcttggt gtaaatttaa tttatggtgc attttataag tacaataatc
     4741 caaaacattt attgcgctat ttgtatgatc accttgataa agaccaactc gaaatagaca
     4801 ctatcaactt ttctggtcct cgttttgcaa atgttgacaa tcgtttaatg agtttgcaat
     4861 tagttaaaaa cggaatgact gatgccgtaa ttttcgaccc cgaaggcaaa aatatattac
     4921 ccgcagcaat tttgtataaa aaaaatatac ttgcgattcg tggaagtttc cgtcccgtaa
     4981 caaaggtgaa tatggatatg tacgaggaat cgttgaagat gtttttgaat gaacaaaaag
     5041 ttaatcctga taacacattg gtcatcttcg aaattacatt gtccaactta cgttctgatg
     5101 gcgaaatcga tgaaaaagat tttatggata gagctcaatt gctatgttca ttaggacaaa
     5161 cagtaatgat ttcgaatttt caagaatttt ataaagtagt tgaatatttc gcaaaatatt
     5221 ccaaagcaag aatgggattg gctatgggcg taaataattt agttgaaatt tttgacgaaa
     5281 aatactatcg tcacctgagt ggtggcatcc tagaagcgtt tggaaaatta ttctatcgag
     5341 atttaaaagt gtatttgtat ccaatgatag acgaaaatgg agaaattcta aattctgaaa
     5401 cattaaaaat acatcctaga atgaaagaat tatataaatt cttcaaatac aacggaaaag
     5461 tggttgatat tgaaaactac aaccctaaaa atctagtggt cttttctaga gaagttctaa
     5521 aaatgattgg agaaagcaaa cttggctggg aatctatgtt gcctgaaggt gttgcaaaga
     5581 tcatcaagaa aaagaaatta tttggttatg accccaatat gcttttggaa aaaaaatagt
     5641 caaaaaaatt gccataaaaa agccaagaaa gtaaattttc ttggcttttt ttagattaaa
     5701 atcttatttt aaaatctgtg cagcgtgggc tttggttttt acatccgaaa ttacttcttc
     5761 gatgattcca ttttcatcaa tgacaaaagt tgttctgtga atgccatcat actctcttcc
     5821 cataaacttt ttaggtcccc aaacgccaaa atcttcaatc actgattttt cttcgtctgc
     5881 caataatgga aaaggaagat cgtatttatc tttgaacttc gcttgcgctt ttgccgaatc
     5941 agcacttaca cctagaagtg cataattatt ggctttgaaa cgttcaaaat tatctcttaa
     6001 atcgcaggct tcggcggtgc aacctggcgt actggctttt ggatagaaaa aaacgactaa
     6061 tttttttcct ttataatctt ctaattgatg gggttttccg tcttgatcta ctccaaaaaa
     6121 tgctggggcc tgatctcctt tttttaatgt tgtcataatt tgggtttata agtttaggtg
     6181 tttaaaagtt tagttattaa attgtaccca aaaataaact aaaatgacca aacaagaacg
     6241 ggtaacattt gttataaata cgttaaaaga attgtatccc gagattccaa ttcctttaga
     6301 ccataaagac ccatacacgt tattagttgc tgttttactt tcggcacaat gtaccgatgt
     6361 tcgggtgaat caaattacac ctttgctttt tgccaaagcc gataaccctt atgacatggt
     6421 caaaatgtcg attgaagaaa taaaagaaat catccgtccg tgtggattat caccaatgaa
     6481 atcgaaggga attcacggtt tatcccaaat tttaattgaa aaacacggtg gtgaagtacc
     6541 gcaaagcttt gaatatttag aagaattacc agccgtcggc cataaaacag ctagtgtcgt
     6601 gatgtcgcaa gcctttggtg ttcctgcttt tccagtagat acacacattc accgattgat
     6661 gtaccgatgg aatttgtctg acggaaaaaa tgtagtccaa accgaaaaag atgccaaacg
     6721 aatttttcca gaagaaactt ggaatgattt acatcttcaa atgatttggt acggaagaga
     6781 atattctccg gctcgaggtt gggatataga gaaagacatc attaccagaa ctattggaaa
     6841 aaaatctgtt ttggaagaat accacaaaaa aacgtcccga tgatttcggg acgtttcctt
     6901 aatttgcaat gatttcgaaa cttttgtcat ttactttaat cacattcagt gctttggagt
     6961 aatttaacaa aaaagaaatg gtttcttttt taggtttcat tttaggggta actaattctt
     7021 ttttagagta aatttttgcc atataatact aaattttagt tttttattag aacgcaagaa
     7081 tttacatttt agtatcaatt cgttaaaatt atttgatgtt tgtctatcac ttttcgcata
     7141 ttcatcagcg cataacgcat tctacccaaa gcggtattga tgctaacacc cgtcgtttct
     7201 gatatttctt tgaaactcat attctgatac attcgcatta ccaatacttc tttttgatcg
     7261 gcaggaagtt cttcaatcaa ttttttcaaa tcgacttcta cttgatcttg aataatttga
     7321 ttttctattg taagcgaatc atcactcata atcgaaaaaa tagaaaactc ctctgtttct
     7381 ctaaacattg gcattttttt gttttttcgg aaatggtcaa taattaaatt gtgggcaatt
     7441 cgcatcaccc aaggcaagaa tttaccttct tcgttataag agtttgattt taaagttcgg
     7501 ataactttaa taaaagtgtc ttgaaatata tcgttcgaaa tatctctatc tgcgattttc
     7561 gaatagataa agccgaaaat tttagattga tgtctattga ttaatacagt caaagcattt
     7621 tcgtcacctt gaatatattt ggttaccaat aaagcgtctg ggagttcgat actagccata
     7681 gtttatacct tttaggttct taataaaatt gaagcaattt tttctaaaaa gtagttctat
     7741 tctataggct aatgtttttt tgtgtttgta tattgggtca aatttaacaa ataattatcc
     7801 taatatacaa ttataacctg aaaatttaac ataaatcagg aaaaataatc tcaaaagagc
     7861 atactatatt ttcgctaaag ccgtttagct agcctaaaaa atgaattcca aatctaaaat
     7921 ttgtgatttg ttcaatacca attctcattt taaaattgat tacttttgtg aaaattcaat
     7981 tttcctatgc caattgacct ttctacactc gatccaaagc aaaatatcat tatcaaaggt
     8041 gcacaagttc acaacctcaa aaatgtggat gtggcgattc ctagaaacaa attagttgta
     8101 attacaggac tttcgggttc tggaaaatcg tctttagctt tcgatacttt gtatgccgaa
     8161 gggcaacgcc gttatgtaga aagtttgtct tcttatgcta gacaattttt ggggagatta
     8221 gacaaaccaa aagtagaata catcaaagga attgctcctg cgattgctat tgaacaaaaa
     8281 gtaaatacaa ctaatgcccg ttctaccgta ggaacttcta ccgagattta cgattatatg
     8341 aaattgttgt ttgctagaat tggcagaact ttttcaccca tttctggaca agaagtcaaa
     8401 aaaaatacgg tttcagatgt tattaaagac gtaaaagagt ttggaacaga cagcaaatgg
     8461 ttgctccttg caccaatcca tttagaagaa ggaagagctt tggaagacaa attgaaagcc
     8521 ttactcaatc aaggtttttc tagaatattg gtcaataatg aaaccgctcg tttggatgat
     8581 ttagaaagtc attcgttaga taacaaagat atccttttga taattgaccg aatcgtagtc
     8641 aaggacgaag aagaattcta caatcgcctt gccgatgcgg tgcaaactgc tttttatgaa
     8701 ggaaaaggaa tttgttattt acaggaatta aacacacata aacgcttttc gtacagcaat
     8761 aatttcgaat tagacggcat tagtttcttg gaaccgaatg ttcatttatt cagttttaac
     8821 aatccttatg gcgcttgtcc ggtttgtgaa ggctacggaa acatcatcgg aattgacgaa
     8881 gaattagtga ttccgaacac agcattatcc atttttgaaa atgccatttt tccttggcgt
     8941 ggcgaaagta tgagttggta cagagacgaa ttggtcaaca aagcctataa atttgatttt
     9001 ccgattcaca aaccgttttt tgaactttcg gaagcacaaa aagaattggt ttggacagga
     9061 aatagttatt ttcaaggttt aaatgacttt ttcaaagaat tggaagagaa aaattataaa
     9121 atccaaaatc gggtaatgtt gtcacgttat cgtggcaaaa ccaaatgcaa ttcctgcaaa
     9181 ggaaagcgtt tgagaatcga agcttcgtat gtaaaaatca attccaaaac cgtttcggat
     9241 ttggttgatt tgccgattaa acatttggtt acgttcttca aaaacatcga tttggatgtc
     9301 tatgaacaac aaattgccaa acgattatta gtcgaaatca ataaccgttt atcgttttta
     9361 accgaagtag gtttagatta tttgactttg aatagaaatt cggcaacact ttcaggtgga
     9421 gaatctcagc gtatcaatct ggcgacttct ttaggaagca gtctggtggg ttctatgtat
     9481 attttggacg aaccaagtat tggtttgcac ccaaaggaca ccgaaagatt aatcaaagtt
     9541 ttactgtctt tgcgggattt aggcaatacc gttattgtgg tcgaacacga tgaagatatt
     9601 atgaaagccg ccgatatgat tattgatatt ggtccagaag caggaacttt tggtggagaa
     9661 ttggtcgctc aaggaacgtt caacgaaatc ttaaaatcga cttctttgac agcaaaatat
     9721 ttgaatggcg aattggaaat caaaacacct cgcattcgcc gaaaatggag caattatatt
     9781 gaaattctgg gggcaagaga gaataattta caaaacatag acgtcaaatt tccacttgat
     9841 gtcctaaccg tgattaccgg agtttcagga agtgggaaaa gtacgttggt caaaaaaatc
     9901 ctgtttccag caatgcagaa aaaactggat ggagcgacaa ccgaaaaggc tggtcaattc
     9961 acagaaatgc gtggctatta ccacaaaatt cagcatattg aatatgtaga tcaaaaccca
    10021 attggacgaa gttctcgttc aaatccagtt acttatatca aagcctatga tgatattcgg
    10081 gatttgtttg ccaaagaaaa actatccaaa atcagaggtt atcaggccaa acatttctcc
    10141 tttaacgtgg acggaggtcg ttgcgaaacc tgcaaaggcg aaggaaccat caatgttgaa
    10201 atggttttta tggctgatgt acaattgcat tgcgaaacct gcaacggcaa acgattcaag
    10261 aaagaagttt tggaagttgc tttcgatggt aaaaatattc acgatattct gacaatgact
    10321 gttgatgatt cgattgcgtt ttttacggct aataaacaaa ccaagattat acaaaaacta
    10381 cagcctttgc aagatgtcgg tttgggttat gttcagctag gacaatcctc ttctaccctt
    10441 tctggtgggg aagctcagcg tatcaaattg gcttcgtttc tggtcaaagg ggctacgaaa
    10501 gaaaaagcac ttttcgtttt tgatgaacct acaaccggat tgcattttca cgatatcaaa
    10561 aaacttctgg cttcatttga tgctttgatt gataaagggc attctatctt agtaatagaa
    10621 cacaatttag atttaataaa atgtgcagac tggattattg atttgggtcc tgaaggcggc
    10681 gaaaacggcg gactattact cgccgaaggt actcccgaag aaatcctgaa aataaaaaaa
    10741 tcggttactg cgaagtattt gaaggagaag ttataaaaac aaaaaataca atttctatcg
    10801 attcgaaatt gaaaaatagt atgctagaaa acgacaacaa cattgaagta ttgggtgcga
    10861 gagtgcacaa tctgaaaaat atagacgtaa aaattccgag agaaaaactg gtagtcatta
    10921 ctgggctttc aggatcggga aaatcttccc tcgctttcga tacgatttat gccgaaggac
    10981 aacgccgtta tgtagaaaca ttttcagctt atgccagaca attcctcggt ggtttggaac
    11041 gtcccgatgt ggataaaatt gacggacttt cgcccgttat tgccatcgaa caaaaaacca
    11101 cgagcaaaag tccacgttcg actgttggaa cgattactga aatttatgat ttcctgcgtt
    11161 tgttgtatgc tcgtgccgcc gatgcctaca gttacaacac aggcgaaaaa atggtttcct
    11221 atgacgatga tcaaatcaaa gaattaatca caaaagattt tgcaggaaaa cgaattaata
    11281 ttctagctcc aattattcga gcaagaaaag gacattatgc cgaattattc cagcaaattg
    11341 ccaaacaagg ttttctgaaa gtccgcatca atggcgagat taaagatatt accactggaa
    11401 tgaaactcga ccgttacaaa acccacgaca tcgaaatcgt gattgacaga atggttattg
    11461 aggatactcc cgataatgaa aaacgtctga ctgaaagcat caaaactgcg atgtatcagg
    11521 gtgaaaatgt tttaatgatt ttggatcagg acaccaatga aattcggttt tttagtagaa
    11581 atttaatgtg tccatccact ggaatttcct atcaaaatcc ggaaccgaat ttgttttcgt
    11641 tcaactctcc aaaaggagct tgtgaccatt gtaaaggttt gggaacggta aacgaaatca
    11701 acctcaaaaa aattattccc aatcctaaat tatctataaa agctggtggt tttgtacctt
    11761 tgggagaata caaaagcagt tggattttca aacagttgga aatcattggc gaaaaatttg
    11821 gcttcaaatt gacggatgca atcgaaacta tttccgagga agcgatggaa atgattttga
    11881 atggtggaaa agaaaaattc tcggttgaat cgaaagtttt gggcgttacc aaagaataca
    11941 aaattgaatt tgaaggaatt tcacatttca tcaaaaatca atacgacgaa agtggttctg
    12001 ccaccataaa acgttgggca aaggaattta tggacgaggt aaaatgtcct gtttgcgaag
    12061 gttctcggtt gaaaaaagaa gctttgtttt tcaaaatcaa tgaaaaaaat attgccgaat
    12121 tgtgcgatat ggacatttcg gatgtaacgg cttggttttt ggaattgaac actcatttat
    12181 ccgaaaaaca aaaaacaatt gctaccgaag taatcaagga aatcaaggac agattgaact
    12241 ttttgatgaa tgttggtttg gattatttgg ctttaagccg aagttccaaa tcactttcgg
    12301 gtggtgaagc acagcgcatt cgattggcga ctcaaattgg ttcgcaattg gtgggtgttt
    12361 tatatattct ggacgagccg agtattggtt tacaccaaag agataatgct aaattgattc
    12421 attctttgga acaattgcgt gatattggca actcggtaat cgtggtggaa cacgataaag
    12481 atatgattga acaagccgat tatgtgattg atattggtcc aaaagcaggt aaatatggtg
    12541 gcgaaatcat cagcattgga acgcctgccg aaactttaaa atcagataca attactgccc
    12601 agtatttgaa tggcaaaatg aaattggaga ttccaaaaaa acgccgggaa ggcaacggaa
    12661 atttcttgaa actgactgga gccacaggaa ataacctgaa aaacgtttcg attgatttgc
    12721 ctttgggcaa aatgatttgc attactggag tttcaggaag cgggaaatcg actttgatta
    12781 acgaaaccct ctaccctatt ttgaacgctt attattttaa tggcgtcaaa aaaccacagc
    12841 cttataagaa aattgaaggc ttggaacata tcgataaagt aattgatatt gaccaaagcc
    12901 cgattggaag aacaccacgt tcgaatccag caacttatac ggaagttttt agcgaaatca
    12961 gaaatctgtt tacaatgact tcggagagta tgattcgtgg ttataaagca ggacgtttta
    13021 gtttcaatgt aaaaggcggc cgttgcgaaa cctgcgaagg ttcgggagtg cgaaccattg
    13081 aaatgaactt cttgcccgat gtatatgtag aatgcgaaac ctgtcaggga aaacgtttca
    13141 accgagaaac tttggaaatt cgttacaaag gaaaatccat ttcggatgtc ttgaatatga
    13201 ccatagacga agcggttcct ttctttgaaa acattccaaa aatttaccga aaagtaaaaa
    13261 cgattcagga tgtcggtttg ggatatatta ctcttggaca acaaagtacc acactttctg
    13321 gcggcgaagc acaacgtatc aaactggctg gagaattatc taaaaaagat actggtaata
    13381 cgttttatat tctggatgaa cccacaaccg gattacattt cgaagacatt cgggttttga
    13441 tggaagttat taataaatta gttgacaaag gcaatacgat tttaattatc gaacacaata
    13501 tggacgtaat caaactagct gattacatca ttgatattgg tcctgaaggc ggaaaaggag
    13561 gtggtgaagt agttgccaaa ggaactcccg aagaaatagc caaaaataaa aaaagctata
    13621 cggcgaagtt tttgaagaaa gagttgaaat aattttgcta ttgaagaatc gaaaaatatt
    13681 tagcgttgtt tttcttctat taatttaaat actcactttt ttaatatatt tgggaggaga
    13741 tttaaaaaca ataattttta gatttcctat taactttttt gatcaaatcc tttaccaacc
    13801 aaaaaacacc aaatgaaaaa aacaaaatca gtactatttt tagcgtttct tttacaaagt
    13861 attttagttt tgggacaaga gcttcctaaa gaaatgcctc ttttcccgaa cggaataaaa
    13921 gacaatccaa ttacccacga acaaaaagaa acttttgcag attctctcgt aaaaaaaggt
    13981 tcgttatccc aaaaagaccg tgttttcaga aatatttctg aaccaacgta tatgttattc
    14041 ccagctgggg aatcgaataa taaacacgtt gctgttatta ttcttcctgg tggcggtttt
    14101 cacaccaatt ggctagataa agaaggaact gatttggctc tatggctttc tgcacaagga
    14161 atcagttgta tggtagtaaa ataccgaacc aattcaaaag acgaaaaagg aaaatacatc
    14221 attccgatgg aaacttattt tgatgatgcc ttaaaaatag acattaacgc aagtgttatg
    14281 acgatgatgg gaatggcaga aaaattaaaa ttcgataagg ataaaatagg cgtcctcggt
    14341 ttttcggcag gcggctttat ggctgaatat ttaactttta caagtgttag taaaaatcta
    14401 ccttggaaac cagcttttac aggtttaata tatgctggag accgattaaa attattggaa
    14461 agtgtaaaag acaaatccgc tcttcctccg ttttttatgg ccattgcaag agatgataaa
    14521 aaatggaaat tacaacgagc acttcctttt ttgtcggctg ttgtggagca agttgataaa
    14581 tcagaattac acgtttattc taaaggagat cacggttttg gattagcgta tgacgaaggc
    14641 cattctgtcg gaatgtggaa agacagtttt tataattgga tattagatat ttataacaaa
    14701 taaccctaaa aaagatattg agaaaaaata ttagttcaat atttaaagaa ccaataaaat
    14761 aatttttaaa cataaaaaaa agcatcatta tatcccataa tggtgctttt cattggtgaa
    14821 acgacacttc acccaagcca attcgcttta atattacaaa gacaactaaa taaaatgtct
    14881 taagaattaa tatgattatc taaaaccgat ttttcattaa tcattttgaa atctaacggc
    14941 tgaatatcat cgaaaccagt actttcaata attttttcgt cttcagcaat aatttcatca
    15001 atagtttttt tggattgaac aaataaaact gaatcagaat ccataaaagc agtgtcataa
    15061 tccatcgata aagtagcttg attataaaca ggcaatgatt tcaaaactgc aattgcttca
    15121 taagcatttg ataactcagc atagttcaat gcggtaaatc cctgactatc ttcaatgtta
    15181 cggtctgcgc ctttttgcaa taagtatttt ataatttcta ctttattata acgagcagcg
    15241 tacattaagg gcgtcattcc atttaatttt ttgttgactt tcgtaccgct ttcaattaac
    15301 tgtttaacag tttcaatttc tcctttacta atcgctatag ctaaagttga tggggtttct
    15361 gaaaatacat tcagtttttt ttgattgcca atactattag tattagaagc caatgacaca
    15421 ctagtaaaag ccattaaagt aattcctaaa taaacgataa aatttttcat tgtaattgca
    15481 tttaaattat tattattttt tttaatgttt gaaagtatct taattctatt aatatctgta
    15541 aatattctta ttagctagta tttcttaaaa acattgaaat gtgaaattaa taaaaatatt
    15601 aacttatttt aaatatatca caaaaaagac attctatata tttaattttt tatcaaatat
    15661 acaattatta aaggtttgac tccaaaaaaa acatcaaaaa aatttactta aaaacctatt
    15721 tttattcttt attcatatca atagttattt cttgaaatta atctattaag aaaaacatca
    15781 ttatttgatt ttttgtgcta tttttagtta ataattagca ttttaaaaaa gaaaaagtgg
    15841 ttcgattgga tgtaaattaa gaataactca aaacttaggc atcaaatacc ttgagtttta
    15901 ttgccttctt ttattgtaat cggacgaata aatagtagaa tttctaagaa attgattaga
    15961 aattaaaaaa gcattgcttc acattttttg aaaaaagaat tactttagca cagaagtctg
    16021 aaatatcctc cttaagatta caaaatcttt ctttagactt taaaaggaaa gaataataaa
    16081 agtttaaaaa atataaaatg agactagaag attttgacaa cgacgaggat aaggtaatcc
    16141 aaaataaatt taaaagaaaa acctggaacg agattcgaac caatgatagt tggggaattt
    16201 tcaaaattat gtccgagttt gtaaatggtt atgaaacaat gggacgaatt ggtccttgtg
    16261 taactatttt tggatcggca cgcataaaac cagaagacaa atattatcaa ttggccgaaa
    16321 aaattgccta caaaataagc aaagcaggat atggcatcat tactggtggt ggtcctggta
    16381 ttatggaagc aggcaataaa ggagctcatt tgggcggagg aacatcggtg ggattgaata
    16441 ttgagttgcc gtttgaacaa cattttaatc cgtatattga tcacgacaaa aacctgaatt
    16501 ttgattattt ctttgttaga aaagtgatgt ttgtgaaata ttcgcaagga tttgtggtaa
    16561 tgccaggcgg ttttggcact ttggacgaaa tgtttgaggc attgacttta attcaaacca
    16621 aaaaaattgg aaaattcccg ataatattag taggaaccac ctattggtct ggattgatcg
    16681 aatggatgaa aaaagtttta gtagaagaag aaaaaacaat taatgctact gatttggatt
    16741 tatttaaaat tgtagatacc gaagatgagg ctcttgctgt tttagataaa ttctacaaaa
    16801 aatatgattt gtcgcctaat ttctaattac acctctgaaa aattatttca aaatttagcc
    16861 tcaaaccttg aaatcattct ataaaaactt cttatttgtt cttttgtttt ttgcgtatgc
    16921 gaaacaatac gggcaacatc attctcagat tgatgtagaa gttaatatgg aacacaaaac
    16981 cctaactgtt ctacaggaaa tagagttttt caatcaatcg gaagatactt tgacttcgat
    17041 agttttgaat gattggaaca atgcctattc ctcaaaaaca actcctttgg caaaacgttt
    17101 ttcggacgag ttttatagag gatttcattt ggcttccgaa aaagaacgtg gaaataccac
    17161 taatttaaac attgcaactg ctgataagaa gaaccttttt tgtgaaagaa ccgaaaaaaa
    17221 tcctgatata attattgtac aattagataa aaaactagct ccaaatcaaa aaattgtatt
    17281 gcattttact tacatcatta aagtcccaag tgataaattt actcattatg gctataccga
    17341 aagaggtgga atgtatctaa gaaactggtt tcttactcct gcacgctatg agaatcattc
    17401 ttttgtaaaa aacagtaatc tcaatttaga cgacattgcc aatgctgttt ctaattttga
    17461 cattctattt agaattccaa aaaacattga attaacaacg gatttggacg aagaaaaaac
    17521 aactccaatc gagaattttt ctattcacaa actcaacggg aaaaacagaa ccgattttag
    17581 tattttttta gaacccaaaa caagttttcg tagcttcaaa aacaatttgg ttgaagttga
    17641 aaccaatctt agaggttata aggctgatga aattcaaaaa gcaattgcaa ttgatagagt
    17701 ggtaaatttc accaatactt taattggaga atcgcacact gaaaaaatca cggtttcgca
    17761 agccgattat gatcgaaatc ctttttatgg attgaatcaa atgcctgctt tcatggtacc
    17821 tttccccgat gaatttctgt ttgaaattaa atttttgaaa acctatttga ataattacct
    17881 caaaaacaat ttgaagttag atcccagaaa agacaactgg attttcgatg gaattcaggt
    17941 gtatgcaatg atggaatata tgaaagaaaa ttatcctgat tgtaagatga ctggaaatat
    18001 ttctaattat aaattattca aaggatacca tcttttaaat ctagatttca acgagcaatt
    18061 tagttatttt tatttgctaa tggctcgaaa aaacttagat caaccccttg gagctcccaa
    18121 aaacacttta ataaaattta acgagcaaat tgccagtaaa tatagggcag gattgagttt
    18181 tatttatctt gacaactatc ttggaaatgg ctcagtagcc aatagtattc agcaatttaa
    18241 caatcgaaat gctataggac aaaccactga aaatgatttt gaaaccctac taaaatcagt
    18301 aacaaacaaa gatattaatt ggttttttaa aaccattatt cattcccgag acattattga
    18361 ttataaattt tcggatgtgt caaaaagcaa agatagtatt tcattttctt tgaaaaatag
    18421 aacaggagtt gctgttccaa tgcccattta tggattaaaa aacgatacta ttgtttttaa
    18481 aaaatggtta gatccccaaa aaaatgatag tatttacacc tttgaaagac atcaggccaa
    18541 taagattgtc atcaattata aaaatgaagt tccagaattc aatttaagaa ataactggaa
    18601 gaaactagaa ggactttttc ctaataatcg ccccataaaa tttgctttaa tcaaagattt
    18661 ggaagatcca ttttacaatc aagttattta tgcccccata ttaacttata atgtatatga
    18721 tggattttct ccaggaatgc gttttcacaa tcgagctatt ttaaatcgtc cttttttata
    18781 tgacatcaat ccgacctatg ctttaaaatc gaaaacacta acaggatcgg ctatttttat
    18841 gatcaataaa gattacagag atagtaattt atttaatgta cgatattcca ttagtggcaa
    18901 ctattttcat tatgcacccg atgcgactta tttgaaaata aaccccatga tgttgatgca
    18961 aattcgcaat gaaaattaca gagacaatag aaaacaacta ctgctttttc gtcaagtaat
    19021 catcaatcga cagcaaagcg acattgttgt ggacagttca cttcaagatt attcagtttt
    19081 tgatgctaaa tatgtcaatt ccagaaccga acttaccaat cacattagtt ttatgggtga
    19141 tgtccagttt tcgggtaaat ttggaaaaat ttctagtgaa gtgcaatatc gaaaactata
    19201 tgaagacaat cgccagctta atcttcgatt gtacacagga gtttttacat acaacaaaag
    19261 caattctgac ttttatagtt ttgccttaga ccgaccaacc gattatctat ttgattacgc
    19321 ctatttggga agatcatcaa gtactggaat tgtaagccaa gaatttatat tggccgaagg
    19381 aggattcaaa tccaaattaa acacacctta tgccaatcaa tggatgacga ctttaaacgg
    19441 aagttattct atctggaatt ggatagaagc ctatggcgat atcgggttgg taaaaaacag
    19501 tcaacaaaaa gaaaaatttt gttatgatgg tggtattcga ctcaatttac taaccgatta
    19561 ttttgaattg ttttttccaa tatactccaa taaaggatgg gaaatttcgc aacccaatta
    19621 caatgaaaaa attcgatttg taatcacgct caatcctgat aaattcattc aactatttac
    19681 cagaaaatgg ttttaattag atttatcaac gcttatttca gatagtttta tattattatc
    19741 atttttttat atatctacat aaatattata tattcaaaat agctatttat aaaaataaat
    19801 tattaaaaat taaattctat ttcttataat gaattatgaa aataactagt tttttaagta
    19861 aatttgccgt aatatatact tcttatacat atgataaaag aaaaagccaa ttcaaccctg
    19921 acctttgaag acttcaaaac cgaagttttg aaagactaca aaattgcaat tacaagtaga
    19981 gaatgcagtt tattagggcg caaagaagta ttgaccggaa aagccaaatt cggaattttt
    20041 ggcgacggaa aagaagttcc tcaattagca atggcaaagt tttttcaaaa cggtgatttc
    20101 cgatctggtt attatcgcga tcaaactttt atgatggcaa ttgatcaatt tacacctcaa
    20161 caattctttg ccggattata tggtcatacc gatatttctc aggaacccat gtcagctgga
    20221 aggcaaatgg gtggtcattt tgtaacacat agtttaaatg aagatggcag ttggaaagat
    20281 ttaacacaac aaaaaaactc tagcgctgac atttcgccaa cagccgcgca aatgccaaga
    20341 atgttaggtt tagctcaagc atcaaatata taccgaagca ttcccgaatt ggccaataaa
    20401 tccaattttt cgaacggcgg aaatgaaatt tcttggggaa ctattggcaa tgcaagcaca
    20461 tccgaaggca tcttttttga aaccataaat gcagctggtg tgctacaagt tccaattatt
    20521 ttgagcgttt gggatgatga ttatggaatt tctgttcatg ccaaacacca aaccacaaaa
    20581 gaaagcatct ccgaaatttt aaaaggatac caaagagatg aaaataacaa tggtttcgaa
    20641 atattaaaag tacaaggttg ggattatcca agtttgattg aaacctatca aaaagcatcc
    20701 gatttagctc gtgaacatca cgttccagtt cttattcacg ttactcaact cacacaacct
    20761 caaggacatt caagttctgg ttcgcacgaa agatacaaag acgaaaatcg attgaattgg
    20821 gaaaaagaat ttgattgtat tcgtcaaatg aaattgtgga tgatagccat taatatagct
    20881 tctccagaag aacttgaaga aatagaccaa atcataagaa aagaagttct cgaagctaaa
    20941 aaaacagcct ggagcgcctt tatcaaccca ataatagaag agcaacaaga attagttcaa
    21001 ttattagaaa aaattggccc aacgagcaaa aatagtgatc gaattcttaa acataaaaca
    21061 aatttaagca atataaaagc acctttaaaa aaggaaattc ttagtacagc gcgaaaagtt
    21121 ttacgactca ttattaatga agaaagcaaa gaggaattat caaattggat tactgcttac
    21181 tttgaaaaaa atcaacctaa atttagcaaa tctttatttt cagaatctaa tttgaatgtg
    21241 ttttcggtaa aagaagtttt acctcaatat gaagaaaacg caaaagaaga tacggatggt
    21301 agaatgattt tgcgagacaa tttcaatgaa cttttcacaa aatatccaga agttttaatt
    21361 tttggcgaag acgcaggaag cattggcgat gtcaatcaag gattggttgg attgcaagag
    21421 aaatttggag aaactcgagt tgccgatgtt ggaattagag aagcctctat tattggtcaa
    21481 ggaattggaa tggctttgcg aggcctacgc cccattgccg aaattcaata tttagattat
    21541 ttattatatg gcgttcaaat tttgagcgat gacttggcca ctttacaata ccgaactctt
    21601 ggaaaacaaa aagcaccact aattattaga actcgtggac atcgattaga aggaatttgg
    21661 cattcaggtt cccctatggg aatgttaatc aatgcgctta gaggaattca cattttggtt
    21721 ccaagaaata tgaccaaagc agctggattt tataacagtt tgttagaatg cgacgaacca
    21781 gctctagtaa tcgaatgttt aaatggctac cgattaaaag aaaaaatgcc attgaattat
    21841 ggcgaattta aaacaccaat tggtgtggtt gaaaccttaa aagaaggaaa agatattact
    21901 ttagtttctt atggatcaac tttgagatta gtagaagctg ctgcaaaaga actaatggaa
    21961 gtaggtatcg attgtgaaat aattgacatt caatccttgc taccatttga tgttcatcac
    22021 gatattgtga aaagtatcac caaaaccaat cgattgttaa tcattgacga agatgttccg
    22081 ggtggagcat cagcctatat tttgcaagaa attatcgaaa cccaaaacgg ttacagctat
    22141 ttagacagca agcctcaaac tctaactgcc aaaccacaca gacctgcata tggaacggat
    22201 ggcgattact tctctaaacc ctctatcgaa gatgttttcg agaaagtata tgctatgatg
    22261 aatgaagtaa acccttcaaa atatccagct ttgtattaga aaatagttct caataattaa
    22321 aacccattac taaactaagt tttctagctt actttagtaa tgggttttct atttaatcga
    22381 atgctaaaaa actactttat aatcttaaat tttttaagat acttgtccat aacctcatta
    22441 atttcagtag aaattacaaa tctataattg agatatacat aagcaatcgt tagcaatatc
    22501 gattttaaaa taatagagat aattggatta aaaggaaaat cccaaatata aaacgcaaca
    22561 aacaaaacaa acgtcagtgc taacgaatat aaggtttgtt tggtaaatgg atacaagtct
    22621 aattttttaa ccacgaaaag taatttggcc aaactgtaca tcgtaatgga taataaagta
    22681 gcaaatgccg aaccaataat tccataaagt ggaataaaga tcatgtttaa tccaacggtt
    22741 aaaatcacca aaagaacccc taaaaacaat actgttcgat agtattttgt attaaagata
    22801 atggcattat tatttcccaa aatcaaatca aaatatttag acaatccaat cataaacacc
    22861 accataatcc ctccagcata ttcgggtgga accattttgt aaagcatatt gatattcaca
    22921 aaaatcccca acattacata tcctccaacc atttgaaggt taattgaagt ttttttatac
    22981 aagatattca tttcgtcgtg cttgttttcg tgcattaatt ttgcggtaat cggatacaca
    23041 atttgatgca tcgcacgact tggaaccgaa atcaccaaag caatataagt tgccactgag
    23101 taaaatgcga tgttttcgat attcatatat tgattcaaaa tcatcttatc gccatccaaa
    23161 agcaaattag ccacacttcc cgacaaaata atataaaaag tataggttaa aatatcttta
    23221 gtattcactg gaattgtaaa ctgaaaatta ggtttttgaa tgctaaaagc ataaaacatg
    23281 gttaccaaaa gcgcaataag ataaacaaca gcagttgcat aaacaaagcc ttcgacggta
    23341 atccaatgaa aatatactcc catcaacaaa aacaaggaag cggttcgcaa acccacttct
    23401 ttgatgaaat tcccaaaaac cgaatgcata tgcactcgca accaagcata aaatatttcg
    23461 aaataggcca tacataaacc tataaaaggg attaaccaaa tatagttttt gactactgca
    23521 ttctttttcg acaaaaagaa taaaatttca tcttgaaaca aaaaccctat gaaaagtaaa
    23581 gggataatca atagcaaagg aaacaaaacg gtaaaagata aaaagcgatt tcgctcggcc
    23641 tctgtttcgt attgcgaata aaacttaacc aaagtatttt gcatcccgat agcaaacaag
    23701 ggcataatta cattggcgct cgaagtgata taatttgtta acccataata agttgcacca
    23761 agaaaaacag gatacaaata aatggtattg attcctccaa tcgcaaaacc gatgtaggtt
    23821 attattgtgt ttttgaacga ctgatttaat actattccca tttatgaggt tagaagttag
    23881 aggttaaaag ttgtaccaat ttctctgtca aactttttct ggaatactgt tgtaaaccaa
    23941 cggcattaga ttgcagtttt ccttccaaaa attgattata atagcccaaa agtacacttt
    24001 ttagtttcat tttctctgaa taatcaaaga atacgcccgt attggtttgg gtaatgattt
    24061 cagcaaagtc agaaccgttg ggaccaatgg ctataattgg tctattagaa accatatatt
    24121 cgaacaattt ccctggaata atacttttgg tgtcttcaga attgatttcg ataagcaata
    24181 aaacttgtga ctttctttga tgagcaatag cttcggaatg cgaaacataa cccaaattat
    24241 tcaaatagga attcaatcca aaattagaaa ttgtttctaa aacttcttga cttacggcac
    24301 caattaattt aatttctaaa tgcgatttga aatctggaat ttcatcaagc aattcgacca
    24361 aagtttccca taacaattgc ggatttcgtt ctgaaagaaa agaaccgatg tgtgccaaag
    24421 aaaatttagc atccaaaggt tgtttctgca cattttcggt gtcataaccg ttcgttataa
    24481 cagcaatttg ttttgttgta atggcttgga actcggtttt tgtggtattg ctagttacaa
    24541 taatagtatc ggcagtattt aaaacttgat gttctaattt tttatgtttc ttggcagcaa
    24601 aatttgacaa tcgcaaggat ttatgatagc caatagttgt ccaaggatcc cgaaaatcgg
    24661 caaaccattt tacattcaat ttttgcttta attccaatcc aatgagatgc aaactgtgcg
    24721 gtggtcccga agtaacgatc gtgtcaatat tattttcgac aatatatttt tctaaaaacc
    24781 gtaccgaagg ttttacccaa taaacacgag catcgggaat aaataaattg ccccgaatcc
    24841 aaagaaatag tttatccaaa aaggattgtt ttttctggtt tggaataatt cccgaactga
    24901 ttttcttggt tttattttta gataaaaacg aagccaattg atagggttca aaaattttat
    24961 gtttgacaac aacagcttta tccgaaactt ctttgaccaa attttcgtca acaattgggt
    25021 aagtgggatt ctccggaata taaacaatag gctgaacgcc aaaatcaggc aaatatttaa
    25081 caaatttcaa ccaacgctgc acgcctggtc caccagctgg aggccaataa taagttatga
    25141 tgaggagttt ttctggtttc aagtttaagg tttaaggttt aaacttttct tcctttcaac
    25201 ataaatcccg ccaattagaa gcaacaacat tccaatgaaa caaaaaagtg taatagtgct
    25261 tcctgtttgt ataacttgag gttcaaactt gaactcaata gtatgctttc cttcaggaac
    25321 tcgcatcgct cttagcacat aattcacagg aaaatgctcc tttttctccc catcgatata
    25381 agcattccaa ccattttcat aatatatttc agagaaaaca gccaatcctt ttcctgaatt
    25441 attggaggta tattttaaat aattcggttt ataaacatcc aattgtatgg ttcctgaatc
    25501 atcaaaatcc tctttcaaac gggcgttttt atatttctta ccataatcgt gaacattgaa
    25561 aaccgcatca tgtttggtgt ccaatttacc caaagctttc atcacatcat caggtttatt
    25621 cactaataaa agatctttaa tgaaccacgc atttccattg gcttctggat tttgaaccgg
    25681 aacagaattg cctgtcgaat cagtttgaat aatgtacttt acattcaaca tattcagaat
    25741 ttcgatattg tttttggcaa tttgataatc gaataattgt tgcattctcc ttggtttaac
    25801 agcgctataa cctcctatcg atttatggaa aaaggatgct ctagcactcg aaagatttcc
    25861 atcaatttca aaaactctat aatgcgactt atcttgcaaa atctgaacat cggcaggtgt
    25921 ttcctgaaat ggaacttcta cttctctttt actaacaaaa ccttcggcat caacatactt
    25981 tttatctaca agaaccaaat caagaatcat caatagtcca actgcaatta ttgtagtatt
    26041 ttgagctaac ttctctttta taaacaacca caaagcagca gctacaagca aaagaaagaa
    26101 tcctgaacgc aacaaatcag ctgaatatag tgtttttcta tcggctttga gcgcatcaat
    26161 aaacgaggga ccataatttt gaatataata accatcgttg gctcctgcaa aactgaaact
    26221 acctttcaat aaaaataaac ccacaataat tccaaaacct actgctgctg attgccacaa
    26281 ggttttgaaa cgtgcttctt tttctaaagt gaaaaaggat tgcaaaccca taatagcaag
    26341 aactggaaaa cacaattcta aaataacttg tatcgacgaa acggctctaa acttatcata
    26401 cattggaaca ttgtcaataa aaaagtcagt caatgtcggg aagttttttc cccaagaaag
    26461 aattaatgca actatagctc ccgaaagaaa gacatattta atttttcgtt tatcggcaaa
    26521 caaagccaaa attcccaaaa agaaaaccac aacaccaata taagctggtg ctgctacaat
    26581 aggctgatcg ccccaataag ttggcatggc tgaaacaaat tcattggctt gtgattctgg
    26641 aactccttgg ctagtcatga atttatacat ttcactatca gttcctaaag cctcactgtt
    26701 tgaacctcca aaaagtcgtg gcgctattaa attgaaactt tcggcaattc cgtaactgta
    26761 ttcggtgata taatcgtgtg acaaagcact gttggtctct gctttttttc catcaggagt
    26821 aaaagtcaat tcactttttc cacgggtact aaattttgca tattcggctg tagccaataa
    26881 gttggtagca ttagcaccaa gtgcaaaaat tccagctatt gccaagactc caaaggaagt
    26941 caaaagtggt ttaaattctt tttcttttat agccaaataa ataaaatatc ccgaaagcac
    27001 caacaagaaa atcaacaaat aataggtcat ttgaaagtga ttggcattga tttctaaagc
    27061 aacggcaaac atagtcagca atccacccca aatatgtttc ttttgaaaaa ccattataaa
    27121 accggcaatc accattggca tataagcaat ggcgtgagct ttagcattat gaccaacacc
    27181 caaaataatg atcaaataag tcgaaaatcc aaaggccaaa gctccaaaaa aggctttgag
    27241 tggatctgtt tttaaaacca aaagcagaac gtaaaaacca ataaaataga gaaataaata
    27301 atccgcagga cgtggcaaga aacgcaaaac atcatcaatc attccaatgt aatcatgagg
    27361 atatttggct cccaattgat acgtaggcat tcctccaaaa gctgaattgg tccaatacgg
    27421 ttcttgatga tttgaagctc taaaatcgtt ttgctccttt gccattccgg tatattgggc
    27481 aatatcagat tgcaaaattt gtttcccttg taaaactgga taaaaataaa tgagagaaat
    27541 taatacgaat ccgagcagca ctatggcgtg aggatataac ttttgaagtt gcttcattaa
    27601 attggttttg tttttttggt tttggttttg ggttttggac aaatttaatc tatctcttca
    27661 taatcgacat aatcccccac ttttttggtt tcgcgagttc ttttggcatt ggctgtatca
    27721 ataattatct catcgttatt tggcgttttt tgccaaggat taccctgagc atattgctgc
    27781 tgtttttgga aattttcacc cgctttctcc actacttttt tgaccaataa tggtaaaaac
    27841 aatcttgcca aaaactttat aatatagtaa atagcaatga tataaattaa agtcctaata
    27901 aatccttgaa atgatgcttc ttgcataatt aaataatttt tggcaaaatt aacaattcca
    27961 tcgttcaaaa ttaaaatatc attcttaaat aaataataaa atatagtaca tttgaaatgc
    28021 gttatccctt tttaaccaaa taaaattata tgtacaaaat caaaaacctt gtcttagctg
    28081 catttttaat gttcccattg atacattatg gacaacacac cgaccaaatc aattcaaaca
    28141 gaccaggcga aacgatgtca gcttttgcag ttggaaaatc agttattcag gtcgaatctg
    28201 ggatttatgg cattcaggaa aatcatagta ttctgaatta tgatgccaat ggatttggta
    28261 ttgatatgac gctaagatgg ggattgttta tggaaaaact agaattgatt gctgatttgc
    28321 aataccaata tgaatattta aatcctgcca tagcttaccc agagaaatca gcgatgaaac
    28381 aaactatttt aggagcaaaa tatttgattt atgatccttt caaaaactac gaaaaaaaca
    28441 tcaatgtgta tagctggaaa gccaatcatt cattcaattg gcatcaattt attcctgccg
    28501 tttcagtttt tgctggggcg aatattacgc tcaatgacaa cccatattat ttttctccta
    28561 aagccgctat ttctccaaaa atagctttga taacccaaaa tcattttggt gacggaactt
    28621 gggtttttgt gaccaatatc attgccgatt acattggaac cgattatcct agttatggtt
    28681 acggcattac tcttaccaaa ggattcaacc ggaaatggtc tggttttata gaaaatcaag
    28741 gctatatgag tgatttttat agtgatgcca ttgtacgtgg tggagctgct tatctaattc
    28801 acgatgattt gcaaattgat gcttccataa gcactagctt gaaagacaca ccctcgatac
    28861 tttatggagg aattggattt tcttggcgtt atgatgccaa atacaaagaa gtacgaatca
    28921 atattgatga tgacaaaaaa acaaaatctc aaaagaaagt tgaaaaaaag aacaaaaaac
    28981 gcaaagacca aatagaaact acatcttaaa aacaaaaatg attaccataa tagaagccgt
    29041 tacaaaaaaa gaaatgaccg agttcgtgaa atttccattt tccttatata aagacaataa
    29101 atattgggtt ccgcccatta tcgccgatga attagaatcc tttgacagaa ccaaaaaccc
    29161 cgctttcgag agtgccgaag cttattttta tttggcctac aaagacaatc aaatcgtggg
    29221 gagaattgcc gccatcatca attggggcga agtcaataac caacacaaaa agaaagtccg
    29281 tttcggctgg ttcgacgtaa ttgacgatat cgaagtcacc aaagcattat tagaaaaagt
    29341 ctatgaacta ggaagaaaaa acaatttaga acacgtcgaa ggaccaatgg gtttctccaa
    29401 cttggacaaa gtaggcgttc taactgaagg ttttgacgaa atgggaacta tgattacctg
    29461 gtacaaccat ccttattttg caagtcattt agagcaattg ggatttgtaa aagagaaaga
    29521 atacatcgaa agcatttttc ctttttcgaa tgtaaaacca gaattcttcc tgaaagcgca
    29581 agctttaatc aaaaaaagat atgaactaag gagtttgaac tttactaaaa ccaaagacat
    29641 tatgccttat gtggacaaaa tgtttgattt gtttaataat tcttacgcta gtttatcttc
    29701 gtttgtagcc attaccgata ttcaaaaaga atacttcaag aaaaaataca tcagttttat
    29761 caatccagaa tacatcaaat ttgtgatgga taaaaacgat aatattgtag cttttagcat
    29821 tgtaatgccc agtttttcag aagctttaca aaaagcaaaa ggcaaacttt ttccttttgg
    29881 attttatcat ttgctcaatg ctagaaaaaa cagcaaagac gttgtttttt acctgatagg
    29941 agttgatcca gaataccaaa acaaaggcgt aactgccatt atttttgacg aatacttcaa
    30001 gacttttagc gaaaaaggaa tcataaactg cattcgaact cctgaactgg aagagaatca
    30061 cgccattcat aacctttgga aaaatttcga cccaagaatt cattgtagga gaaaaacgtt
    30121 tattaagaat ttgtagaaaa ctaggctccg caaagtctcc gaccaagact ttgcgaagcc
    30181 atttttcaga atgctacaat tacaaaaaat ccgtttttat ccgctctatc tgcgtcatcg
    30241 gcgttccatt tttttaccaa taaactttgt aactttgcct ttctgaataa cgccaaatga
    30301 aattccaagt catatccgat taccagccca aaggcgacca accccaagcc attgccaaat
    30361 tagcccaagg cattgaagct gacgaacgct atcaaacttt gttgggagta acgggttctg
    30421 gaaaaacatt taccgttgcc aatgtcattc aagaggtgca aaaaccaact ttgattttgg
    30481 cacacaacaa aaccttggcg gcacaattgt actcagaatt caagcaattt tttccgaata
    30541 atgctgtaga atatttcgtt tcttactacg attattacca acctgaggct tttatgcccg
    30601 tttctggagt ctttatcgaa aaagatttat ctattaatga ggagttagaa aaaatgcgtt
    30661 tgagcaccac ttcttcccta ctttcgggac gaagagatat tattgtggtg gcatcggttt
    30721 cttgtattta tggtattgga aatccagtag aatttcaaaa aaatgtgatt gcgattgaga
    30781 aagaccagat gatttctcgt accaaattat tgcacagttt ggtgcaaagt ttgtattcca
    30841 gaaccgaagc cgattttaat cccggaaatt tcaggatcaa aggagataca ctcgaagttt
    30901 atccaagtta tgccgatgat gcgtatcgaa ttcatttctt tggagacgaa atagaagaaa
    30961 tagaaagctt tgatttgaaa acatccaaag tgattgagcg ttttgaaaaa cttacgattt
    31021 atcccgccaa tatgtttgtg acttcgcctg atgtcttaca aaatgccatt tgggaaatcc
    31081 agcaggattt ggtcaaacaa gttgattatt tcaaagaaat tggtaaacat ttggaagcca
    31141 aaagattgga agaacgcacc aatttcgatt tagaaatgat tcgcgaactg ggttattgct
    31201 ctggaattga aaactattct cgttaccttg acggacgaga tgctggatca agacctttct
    31261 gtttgttgga ttattttcca aaagattatt tgatggtggt ggacgaaagt cacgtgacgc
    31321 tttcgcaggt tcaagcgatg tacggcggcg accgcagtcg aaaagaaaac ttggtcgaat
    31381 atggcttcag acttcctgct gcaatggata atcgtccgtt gaaatttgag gaatttgaag
    31441 caatgcaaaa tcaagtcatt tatgtttcgg caacgcctgc tgattatgaa ttgcaaaaat
    31501 gtgatggtgt ttatgtggag caaatcattc gccctactgg attattggat cccattattg
    31561 aaatccgtcc gagtttgaac caaattgacg atttgattga agaaattcaa acccgagccg
    31621 aaattgacga acgggttttg gttacgactt tgactaaaag aatggctgaa gaattggcta
    31681 aatatttaga caaagtttcc attcgttgcc gctatgtcca ttcggatgtg gatactttgg
    31741 agcgcatcga aattatgcag gatttacgaa aaggattatt tgatgtttta attggcgtga
    31801 acttattgcg tgaaggattg gatttgcccg aagtttcctt ggttgctata ttagatgctg
    31861 acaaagaagg atttctgaga agtcatcgtt cgttgaccca aaccataggt cgtgcggcaa
    31921 gaaatttgaa cggaaaagcc attatgtatg ccgataaaat cacggcaagt atgcaaaaaa
    31981 ccattgacga aaccaattat cgcagaacca aacagattaa tttcaatacc gaaaacaacg
    32041 ttattccaat ggcgttgaac aaaaaaatag agagtgcttt taccaacaat aaattggtag
    32101 aatatgaatt aggacacatt ccttcgcttg ctgccgaacc tgaaacggcc tatttatcaa
    32161 aacccgaaat tgaaaaactc attcgtgaaa aacgaaaagc aatggaaaaa gccgccaaag
    32221 atttggattt tatgcaagca gcaaaattga gagatgctat aaaagctttg caggaacaat
    32281 taagctaagt tattagaatc acataatact aatcacctct taatgtataa ttattaattc
    32341 gtaattattc tttaattgtg ttgctcttta aaaatttcca ataaaacttc cgcttcaatt
    32401 acaggacttt gggtcttttt catcattttc agaatacgat tggttactat tgggttcagc
    32461 cccatatcga ttcctatttg ttgaatggca acttcttctt tcggatgtaa aactccgtca
    32521 cagtgcatca ataaagccaa acgataaaac tgatgaaagc gttcaaattc agatttgata
    32581 acaataaagg gcaattcctg atggaataaa tcatcaaaaa cttctttggt aatcttcaac
    32641 tcatcagcaa caatggataa aaatgtatat tcttttttat gcaaacttcc gtcaattgta
    32701 gcaaaagcaa tcatttctaa aagtaaacta attttttctt cgtaagtagg catcaaattg
    32761 ttatttttag ctaaaatatt gttttttatc taaatcacaa aatctggcaa tgatacaacg
    32821 atttactgta gttataattt tctttattgc aataggaaca acttttgctc agcaaagcac
    32881 cgcttcaaaa caagtttcta cttttactat agaagcacct caattgcaca ctacaaagaa
    32941 aatttggatc tatttgccaa agaattataa atcatccaca aaaaaatatc ccgtgattta
    33001 tatgcacgat gcccaaaatc tttttgatgc taaaacttcc tttgtaggcg aatggaatgt
    33061 agatgaaaaa cttgatagcc tcaatgcaca agtgattgtc gttggaattg aacacggaaa
    33121 cgacaaaaga atggaagaat taacacccta caaaaacgct aaatatggcg gaggaaatgc
    33181 agataattac cttgatttta ttgtaaaaaa cctaaaacca gcaattgaca ccaaatacag
    33241 aactaaaacg aatgccagaa atacagcaat tatgggaagt tcactcggtg gattggtttc
    33301 ttattatgct attttgaaat atccagaagt ttttggaaaa gcaggactat tttcaccttc
    33361 tttttggttc aatcgaaagg aaatcatcga attaacaaat tcaattccga agctaaaaac
    33421 caagatttat tttctttgcg gcgacaatga aggtgatgct gatatgataa atgacctcaa
    33481 caaaattgac gaattaattt cagaaaaacg ttgtgaatgt gtgcaattaa ccaaaaagaa
    33541 aatcgtcaaa ggcggtcaac ataacgaaaa attatggcga gatggatttg caaaagcgta
    33601 tgtttggttg ttttaaaata tccatttaaa ccatttataa atcaaaaata tggtcaaaat
    33661 acagataaaa aatcgagttt cgactttact aattttattt ttggtaagca cagctacatt
    33721 tgcgcaaaaa atacccgaac gaaaaatcaa aaaattaatt gaaaaatcag aaattctgaa
    33781 cgaacatttc acaggttttg ccctttttga ccaagaccaa gacaaaatga tttatgagca
    33841 aaatgccagc aaacattttg ttccggcttc caatactaaa atatacactt tttacaccgt
    33901 tttaaatatg ttgggcgatt cgattcctgc gttgaaatat gtaaccaaag gcgattcctt
    33961 gattttttgg ggaactggag atccaacact aacttattcg acatttaaat cgactaaagt
    34021 tttggatttt ttgaaaaaat cggaaaagaa attatacttc gcatcccaca actacaaagg
    34081 gaatttttat ggatatggct ggaattatga cgattattta gaagattttc aacccgaatt
    34141 gaccgctttt ccagttgatg gaaatactgc gaaagtatat aaaaccgaaa aaggtgaagt
    34201 gactgtaaaa cctccatttt tacaacctta ttttaaagct gaccccaatt atatccctga
    34261 aaacttcctt attcatcggg atttttatac caatcagttt acgtatccaa cgacaattgc
    34321 taaaacagga tatcttcaag aaattccgtt taagaccagt cctgaactaa caatttcgct
    34381 tctggaagaa aaaataaata aagaaatttc gctcctaaac attactttac ctcaggattt
    34441 caaaatcatt tacagcgaaa aaaccgatga tgttttaaga aaaatgatgc tagtcagcga
    34501 taattttata gccgaacaat tgcttttagt ctgttcttca acgctatcga atgatttatc
    34561 taccaaagta ataattgatt atagcaaaac taattttatg aaagagtttg ccaatgatat
    34621 agtttgggta gatggctcag gcttgtcgcg atataattta ttcactccaa atacctcaat
    34681 tattttgcta aaaaaaatca gtcttacaat taatgacgag gaaagattgc acagtttatt
    34741 tcctgctggt ggagtttcgg gaacacttaa aagagcctat aaaactgacg ctgacaaacc
    34801 ttttgtttgg gcaaaaacag gaacacttac caatgtttac aatcagagtg gttacatcat
    34861 tactcgaaaa ggcaaaaaat taatctattc attaatgaac aataatttta ccagaaccac
    34921 tgaggaaatt agggatgaaa tggctagaat tatcactaaa atccacaatg attattgatt
    34981 cccattttca attaaggaat aatttacttc caaattgctt ttaaaccgta cctttgtaaa
    35041 aaaataaaaa atgacaaaca acgatatttt caaaaaactt cgagtagcat taatgctgcg
    35101 tgacgaccaa atagtagaaa ttctagaatt agtagatttc agaatttcaa aaacagaatt
    35161 aagtgctttt tttcgtgccg agaatcacga aaattatgtg gaatgtggcg accaagtgtt
    35221 acgcaatttc ctaaacggat tagtgattca tttgagagga actaaggata atccaaaaaa
    35281 tccaaatgat gttttggcca aacacaaagc acaaatcccc aagaaagaag gaaactcaga
    35341 tagacccgaa tttaaggcaa aacccaaaga cgaagaacga gtcagaggtg accaaagccc
    35401 ttctaaaaca aaaccagcag caaaaaagaa gcctttcaaa aaacctgctc caaaagtgca
    35461 agttgtggag aaagtaaaat acaatttcgg taaaaataag aagtcataag tcaaaaacat
    35521 tcacttaccg caagtttaaa atactttttt taatcttaac taaacattga tgaaatcctt
    35581 aatatcaatt gcctttttat ttttaaccat aaatgttaaa agtcaaaacc agttaaaa
//
LOCUS       DAOSNN010000002        33079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_1370_length_33079_cov_4.972777, whole genome shotgun sequence.
ACCESSION   DAOSNN010000002 DAOSNN010000000
VERSION     DAOSNN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 33079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33079
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1370_length_33079_cov_4.972777"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3145)
                     /locus_tag="PLQ70_00150"
     CDS             complement(<1..3145)
                     /locus_tag="PLQ70_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33056.1"
                     /translation="MTTDNKVNQEKIKQSRKDTVQYINLQLASLGQPIYRDDENSTEP
                     FCNPKFQTLTNGLIKSFREKSRLLSNHLSPADNRIQNFLNDYLKGVPFNKPYVLPNDT
                     LILTQSGHARELSLPPNGTTFASDDITSYRIRQGILNNPVNDKRTTIGTFHIVEGNLP
                     APLDKKEVPKIAFAYFLNAAFNPSDKLKLLPFTSNQEEKAKLMVSLLMRPMVCPEVKG
                     VISKKSMEVRFFAPGSLVSNLDFVEAIFGNAGDPYLAQNDAALDTEHWTGHTGCVILA
                     PQLRLLKKKDIGLPHFDDATERQKKEGMCWKDENELYNNGGAFKVTCRDDRGVVVTII
                     ADNYFGYSKKEIKTQISYSANLFGLVEEEHSGGAIAYPRGVMGDIVDGSAFSEKFGNQ
                     YNFEGVKAFLGDRIEVKPINYAVDKKYPNLVYIPENAYINTNTNSITWTFDGKEQKLI
                     LSPNKTYIHPTGNKFKLEKHNAIALWRIVNTSPEGVFCHKPCTVSGGGKSEISKSMQN
                     AITYSAFNILDIDEDFKKADEIIEYNYSKRWKDSDPSLPPSRSFLDKGRTLGSAVKLL
                     TPSDRNTDEYNEFIKNIPVHIRSLVLFVKRLYRQDHGALNWKDCMSVEIINGKNGTGL
                     QYNNTPVVGSYVRIGFNQKGTWLLNKLRSDFSASQKIQMEDDISASITLPSHQIKNLN
                     PEYTNQSLKVVANCESHLFQRPDEAIVRGYDKGAELDIVSDNVFLTNYELLRKKDAIE
                     IFEDTINFDKYTQPVKDLILRIVNSPNEEEYFSLPSHTRIVDGEPTKNPRYLERNIFI
                     DETEASYLGEIGVRLYRKIKSEDPVINVVNAVLPGRRNNPIDKKAGIRPLAVYNPIHY
                     QETPELFMDFICSLTGKSPSTTGAGSEGALTKGPFNMLTPTSDLNNALLSHILTESNA
                     FSTAAGYVGGENKVDHDISLLIPEIWARLDPKDRDPKVLMANGSLEKLEDFEYQGKKV
                     LASRLGYRITSTFSLRCLNRLFDEPNAVFNEKMLKPELQGIEDYVDGIANIVEAQQKV
                     ALSYFEDGSIEAAIPPLK"
     gene            3381..3842
                     /locus_tag="PLQ70_00155"
     CDS             3381..3842
                     /locus_tag="PLQ70_00155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQE33057.1"
                     /translation="MKKLLPLLLLFTLFISCSKDDAQIVDYTAQNEKEITDFIALNKL
                     NAQRSASGLYYVINNPGTGTQPTATSNVTVAYKGTFTNGTIFDQSTNAGISIGLNQVI
                     KGWTEGIQHFKPGGSGLLLIPAHLGYGSYNYQGIPGGSVLIFEISLISVNQ"
     gene            4071..5324
                     /locus_tag="PLQ70_00160"
     CDS             4071..5324
                     /locus_tag="PLQ70_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQE33058.1"
                     /translation="MDTKTILEAKSICFLLIFTFYFQLSFSQEEGEKKQPLRTILDLF
                     TKEDTLKIKLPKTKTKLIAFPTLGYQPANGFTLGFISQYSFKEKEENKISLISGGASL
                     STKKQLLTYLKNYMYLDDDRYFFSGDFRYYIFSQSNYGLGTDIVPWGAEFKDFDYKSI
                     EQPMNYNYFKFHETISFNIFPSFFIGTGIHIDSYNNINDKLLDVSQEEYTYHYNYSKK
                     YGFSNKNYAVNGLSMNLIYDTRDNLINTNNGLYLNLNYRYNPKLFKGQQKSSSLLLES
                     RYFIPISKRNKQHVIGFWGYSQFVTSGHLPYLNLPAIGWDQNSRSGKGYIQGLFRGNN
                     LIFLESEYRFPITKNQMISGTVFANATTASDANKNLRLFESIQPAVGIGLRFLLDKNT
                     LTNFVVNYGLGRDSQTFYFNDGEGF"
     gene            5664..>6549
                     /locus_tag="PLQ70_00165"
     CDS             5664..>6549
                     /locus_tag="PLQ70_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33059.1"
                     /translation="MEIEIKNFGPIDSLKFNLNKDLHLIYGQNAIGKSYATYCLYCLI
                     KNIKNKSIGNRHMYFYDKRDENAYEKFIKSKIKSLKTNKSIDCTKEFNTLIEIELKEI
                     ILKGLQNSLLNTFSSLKNLKNRYSDKNYELIISISKYEKLSILSNDEGELDLKYENEI
                     KKIELFQKETKSTKYSLILNNRKEFGKPTEDEFIHEFIRFSYVKINQILRKLDFNIKD
                     VYYLPASRSGLYQALSAFTPIIAELTQNRFFIQNKSIELPSLSEPLADYFIDLSTVDK
                     KHNNKEFEGIVKLLQNEIL"
     assembly_gap    6550..6559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6560..7121
                     /locus_tag="PLQ70_00170"
     CDS             <6560..7121
                     /locus_tag="PLQ70_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQE33060.1"
                     /translation="KVDYNDETRRISYQPDGIDLELHLSEASSMVSELSPLVLYFKHI
                     LNNKYSSINSRDSFFFDPFYNDSFGKRKNKGYDILFIEEPEAHLHPEVQVKLIEIFAK
                     LTGLNLKIFITSHSNYMFNKINNILIKKEIEKEKVMVYHLIKTGNGTTTNIEMKVTED
                     GINDSNFQDVSEQLYYERLNYLEKND"
     gene            7114..7620
                     /locus_tag="PLQ70_00175"
     CDS             7114..7620
                     /locus_tag="PLQ70_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33061.1"
                     /translation="MINNIISHDTLSQYIEDTCCENGVCVSFEDDIDPNSYVIIKVDQ
                     FYNSLNIEKRPASIDCLIIRKCLISGYGLTLVELKNIENSKGFDLENMKSKFDTTLND
                     FIKNRFRDPLDIDYKDIKLYFVSKQEIYRRDLGLKMEVLMNVRFKFNEKQLMIIPRMP
                     NPTIKKCY"
     gene            complement(7816..9366)
                     /locus_tag="PLQ70_00180"
     CDS             complement(7816..9366)
                     /locus_tag="PLQ70_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE33062.1"
                     /translation="MVNEEIENKTTISSEEIDRCIAILAQLNTDTDQIFEIPKEQRIA
                     LIKAAGQFSRPDRDEFAKRKKDGKAVAKRKQEKKDRTARKETGIRSAREASVFVAPKL
                     LASHDLEHKEQLELETPKKCYVCKTEFTKMHHFYDTMCCDCGDFNYAKRFQTADVNGQ
                     IAVITGSRLKIGYHITLMLLRGGATVIATTRFPVDSALRFSKEDDFNEWGHRLKIHGL
                     DLRHIPSVEIFCNFIEQKYGRLDILINNAAQTVRRPAGFYTHLIENEERPIASLPQKA
                     QELLLDHTNCLEELKVLTLGASSNENMPVTWHGPEPGIGLRASAKLSQIPYSFDNALV
                     AQEVFPEGELDADLQQVDLRKVNSWRLKLGQIETTEMIEVQLVNSVAPFVLCNRLSEV
                     MKKDHTGKKHIINVSAMEGKFYRDFKEDRHPHTNMAKAALNMLTHTAAGTLAKDGIFM
                     NAVDTGWVTDEDPAELAKRKQEEQDFQPPLDIVDGAARVMDPLFDGINSGKHWSGKFL
                     KDYRPIAW"
     gene            9571..10362
                     /locus_tag="PLQ70_00185"
     CDS             9571..10362
                     /locus_tag="PLQ70_00185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQE33063.1"
                     /translation="MLFQIQSYLKFLWHSKNEHAVHSPFVFNLITKCFYDKKSKPQYA
                     VLKEYRNSLLANKNTIEVTDFGAGSKVFKSNTRQINKIAKTAGISPKRAELLFRITNY
                     FKPKNILEIGTSLGLATSALALGSRSAGTKANIITLEGCPNTANQCQLQLQKFNINTI
                     DLVVTEFTSYLNNCQLKTDHCQLIYFDGNHSKTSTLEYFELLLPTITNDSVWIFDDIH
                     WSPDMEEAWEIIKKHPKVTVTIDTFQWGLIFFRQEQPKEHFVIRV"
     gene            10486..11172
                     /locus_tag="PLQ70_00190"
     CDS             10486..11172
                     /locus_tag="PLQ70_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE33064.1"
                     /translation="MANPLIKIESIKRDFALGNEIVYVLKGIDLEIEKGEYVALMGPS
                     GSGKSTLMNLLGCLDTPTSGSYILNGKDVSQMQDDELADIRNKEIGFIFQTFNLLPRT
                     TALDNVALPMIYAGHSKSERNIRATEVLKQVNLADRMDHQPNQLSGGQRQRVAIARAL
                     VNKPSIILADEPTGNLDSKTSEEIMALLEEIHQKGNTIIVVTHEEDIAAHAKRIIRMR
                     DGMIESDTSK"
     gene            11244..11813
                     /locus_tag="PLQ70_00195"
     CDS             11244..11813
                     /locus_tag="PLQ70_00195"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQE33065.1"
                     /translation="MKIYTKTGDKGTTALFGGTRVPKDHIRIESYGTVDELNSYIGLI
                     RDQEINSHYKNILIEVQDRLFTVGAILATPPEKEVKKNGQLRLQKLGIIDSDIELLEN
                     EIDVMEESLQPMTHFVLPGGHTTVSYCHIARCVCRRAERIAVHLDHNEPVSEIVIRYL
                     NRLSDYLFVLARKLSSDLNAEEVKWIPRK"
     gene            11994..12215
                     /locus_tag="PLQ70_00200"
                     /pseudo
     CDS             11994..12215
                     /locus_tag="PLQ70_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441576.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
     gene            12301..15642
                     /gene="secA"
                     /locus_tag="PLQ70_00205"
     CDS             12301..15642
                     /gene="secA"
                     /locus_tag="PLQ70_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQE33066.1"
                     /translation="MSFINSIIKAFVGDKSQKDVKALQPYLAKIKTFETALAALSNDD
                     LRARTTSFKEKIKQARADKDAKIATLQLQAENTEDIDKREDIYVEIDALEKEAYEISE
                     KTLMEILPEAFAVVKETAKRFKENTQIQVTATPLDRELSATKSYITLDGENAIWANSW
                     SAAGKAITWDMIHYDVQLIGGMVLHEGKIAEMQTGEGKTLVATLPIYLNALTGNGVHL
                     VTVNDYLAKRDSTWKAPLFEFHGLTVDCIDNHQPNSEGRKKAYNADITYGTNNEFGFD
                     YLRDNMAHSPDDLVQRKHNYAIVDEVDSVLIDDARTPLIISGPVPEGDRHEFNELKPK
                     IENLVALQRQLANGFLSEAKKLIKEGNTKDGGFLLLRAHRSLPKNKALIKFLSEEGIK
                     QLLQKTENQYMQDNNREMPKVDEALYFVIEEKNNQVELTDNGIKFLSGDTDSNFFVLP
                     DIGTEIAIIEKQNLEKDKEAEEKEKLFQDFGVKSERIHTLTQLLKAYTLFEKDVEYVI
                     MDNKIMIVDEQTGRIMDGRRYSDGLHQAIEAKESVKIEDATQTFATVTLQNYFRMYSK
                     LGGMTGTAVTEAGELWQIYKLDVVEIPTNKPMLRKDKEDFIYKTTREKFNAVIDDVTE
                     LSNSGRPVLIGTTSVEISELLSRMLKMRGITHNVLNAKMHKQEAQIVEEAGKAGVVTI
                     ATNMAGRGTDIKLTPEVKAAGGLAIVGTERHDSRRVDRQLRGRAGRQGDPGSSQFYVS
                     LEDNLMRLFGSERVAKVMDRMGLQEGEVIQHSMMTKSIERAQKKVEENNFGVRKRLLE
                     YDDVMNAQREVVYKRRRHALHGERLKLDIANMLYDTCDLIVTENKLTHDFKNFEFELI
                     RYFSITSPVSESDFNKLTDIELTGKVYKAASQFYAEKTERSAREAFPIISNVYEDKNN
                     QYERIIVPFTDGIKSLNVVTDLKKAYETNGNQLVADFEKNITLAIVDEAWKKHLRKMD
                     ELKQSVQLAVHEQKDPLLIYKFEAYNLFSSMLNGVNKEVISFLFKGDLPQQQAPAIQE
                     AKQVRQKENYTESKDEIVSSESANREAGQTQQRQVTETIVRDQPKINRNDTVTIQNVA
                     NGQIQEMKYKKAESLIANGTWVIVS"
     gene            15742..16266
                     /locus_tag="PLQ70_00210"
     CDS             15742..16266
                     /locus_tag="PLQ70_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33067.1"
                     /translation="MKNLIASITLLLITTTSLSAQTNTKEYKIGHVVNVSLPDYMTRT
                     MGLNSAAMFQYKNTIKDVYGFIIEDNKEELALAEMKYSSINEFCEEFAKDFLKDEKKK
                     TFSYPEYQKKGDISFAEFDATYYDKDIKGEVYYLVGIVETKTAYYKVISFTSKANKDK
                     FKADFQSILYSVND"
     gene            complement(16641..18104)
                     /locus_tag="PLQ70_00215"
     CDS             complement(16641..18104)
                     /locus_tag="PLQ70_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HQE33068.1"
                     /translation="MSQEIRNLEPKALWNKFADLNAVPRPSKKEERVIEFMKNFGNSL
                     GLETFEDEIRNVIIRKPATAGMENRKTVVLQGHLDMVHQKNADTNFDFDTQGIDMYVD
                     GDWVRARGTTLGADNGLGVAMIMAILESKEIAHPAIEALFTIDEETGMTGALNLKGGI
                     LKGEILLNMDTEEDDEIDIGCAGGIDVTATRSYQEEETPEDSVGYTITVKGLNGGHSG
                     MDIHKGLGNANKIMNRLLFDAFENFGLQVAEINGGSLRNAIPRESVAKVIVAGMYDEA
                     YVFDMQEIISDIKTEYKTTEPNLTIEIVKCDLPKKVMDLGVQEGILRSIYAAHNGVYR
                     MSADMEDLVETSNNVARVIIKDGEISISCLTRSSVETSKFDLANALRSAFELCGCEVT
                     LAGSYPGWTPNVKSEILDLLVKIYEKQNNEKPKVVACHAGLECGILGTNYPDMDMISF
                     GPTIQGAHSPDERASIKSSQKFWKFVIEILENIPLTK"
     gene            18213..19274
                     /locus_tag="PLQ70_00220"
     CDS             18213..19274
                     /locus_tag="PLQ70_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HQE33069.1"
                     /translation="MQRKYILPLFLLIQILFLKIIAFFPGWVERFYSNGLYVLISKFS
                     RIVLGIIPFSVGDIMYGILIFYLLKSIWKARKTWKTEWKNNLLKVLNVLSVAYFLFHL
                     LWALNYYRQPLFEKMQIERDYNNADLFDFTKKLIAKTNQIQSQITKNDSKKVVFPYSQ
                     KQVFEMNLNGYKTLSNEYHFFEYTNPSIKKSLFSLPLTYMGFGGYLNPFTNEAQVNYL
                     GPMYGFPMTTNHEMAHQMGFASESECNFIGFLASVKNENLYIQYSGYSNALRYCLGNW
                     YVRDEKVYDQLLKTIHPGILKNYQENEDFWKQYETPIETGFHIFYDNFLKINQQKDGM
                     DSYSKFVNLMVNYYKGKKL"
     gene            19434..19928
                     /locus_tag="PLQ70_00225"
     CDS             19434..19928
                     /locus_tag="PLQ70_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQE33070.1"
                     /translation="MSDDLQQSFVLQLKTNQNIIHKICRLYTNGEDAHKDLFQEITIQ
                     LWKAFPKFRGDSKFSTWAYRVALNTAITLYRKSTRSVKTVDYETQQYFISNEDYNYEE
                     EERLKLMYKAVYQLNDIEKALIFMYLEDKDYQEISETLGISEVNARVKMNRIKGKLKK
                     ILNP"
     gene            19933..20535
                     /locus_tag="PLQ70_00230"
     CDS             19933..20535
                     /locus_tag="PLQ70_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33071.1"
                     /translation="MKELDLLKRDWHKNNAFEQVSEVEIYKMLHQKSSSIVKWILIVS
                     LIEFVVINGISLLLNDPKYDEFISNHPYINYLEKFNYVVIVAFIYLFYRNFKNISVLN
                     SSKTLIKHILKTRKIVTYYIYWNIFIGGITGALSGIEGFNEGFKSGGGDNAVLATNCL
                     TIIIMAALIMGFIWLFYKLLYGSFLRKLKDNYTELKKIDL"
     gene            complement(20550..21359)
                     /locus_tag="PLQ70_00235"
     CDS             complement(20550..21359)
                     /locus_tag="PLQ70_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQE33072.1"
                     /translation="MALFKRNPFGHILVVKRFLIHVFAVLTHRRYRGFNSLQIEGSEI
                     ITKLPDANVLFISNHQTYFADVVAMFHVFNASLSGRQDSIKNIGYIWKPKLNIYYVAA
                     KETMQAGLLPRILAYIGAITVERTWRSCGKDVTEKRDVNPNDTENIKIALNDGWVITF
                     PQGTTKSFKPVRKGTAHIIKQHRPIVVPIVIDGFRRSFDKKGLRMKKKDILQSFIIKP
                     PMEIDYDNDTIEQIVEKIEYAIEQHPSFLKVIPAEELKAAEELNKLRQWDY"
     gene            complement(21413..22051)
                     /locus_tag="PLQ70_00240"
     CDS             complement(21413..22051)
                     /locus_tag="PLQ70_00240"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQE33073.1"
                     /translation="MDFQEFLKYVPKLVNVNLPAKEAHFKMAPLERIESLKNQNLDSF
                     NPKTAAVMMLFYPKKGRTHLVLIVRNAYEGIHSAQIAFPGGKYESNDQTFVNTALRET
                     QEEIGIHPEQIEIIMPFTNLYIQASNFMVYPFLGISKEEIVFIPDSNEVAQIIELPLS
                     DFLNDNLIVSAIMTTSYAKDIEIPAYKINEHIVWGATAMMLSELKEVLKKVL"
     gene            22185..22628
                     /locus_tag="PLQ70_00245"
     CDS             22185..22628
                     /locus_tag="PLQ70_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HQE33074.1"
                     /translation="MYSKEEAQRLKREFWIEFAEKYPRKWILYDTKIKDFSFKFYVDN
                     KKAQVLIDIEHRNDEKRIVYFEKLESLKTVLEDDFVKDLVFEKNFTLESGKTISRIWV
                     EKTGISVSNRKYWDEIFDFFYEKMNALEMFYFEYGDYIRDLELNT"
     gene            22693..24606
                     /locus_tag="PLQ70_00250"
     CDS             22693..24606
                     /locus_tag="PLQ70_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HQE33075.1"
                     /translation="MKKIYWVFFVVLFTFTSLAQNSLEDYKKKYPDFNELILKDQQAY
                     DFSIENDKLKVIENSQYESLIMTDNGIINNKESFSYSELVKLMGYDAYSVVTENGKEK
                     KIKVTQTNEVQSGQSSVFYDDVKVRQLIFPNLEAGAKKVYHYQTEIIDPFLLHKYVFG
                     SNLPIKNAVLEIKTDKNINIGYKIFNDPTNSIEFSKTEKKGKWIYQWTLKEVKPVKFE
                     SNSPGFLHLVPHINVYIKDYTINNKKVEVLGDIDKLYTYYKGFTKNINQKEDANLKAI
                     TLKLIENLTTDEEKIKSIFYWVKDNIKYIAFENGYEGFIPREASLICERKFGDCKDMA
                     SIITTMAKYANVKDVFLSWIGTREIPYSYNEMPTPAVDNHMIAVYKKGDNYIFLDATD
                     KETLYGIPTSFIQGKEALIGEGENYKIVTVPIVAAETNEVNEQVKLSIEKDKLVGSGK
                     VQFKGYSRSNITLRMGDATNKTRFEMIKSLVLKGNNKFNLKEYSEENLTDRDKPYQIN
                     YNFDLQNYIVKADKEIYINLFLEKPFEKLTIEKDRFAPFEFEHLTFVNSQYELEIPKN
                     CSLKYLPKNFSLDNDYVKANFTYQNQNNKITLSIKLQQKKLLIAKSDFELWNKTIKEL
                     KNNYSETLILLEK"
     gene            24606..26576
                     /locus_tag="PLQ70_00255"
     CDS             24606..26576
                     /locus_tag="PLQ70_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HQE33076.1"
                     /translation="MKINPLKIIFTLVVLFNSSAFLAQEYSFKSYDWNEKETTVSVPE
                     KYKNENEVILNRTEKIEFVIVGKTAKQYHLVHEKIFINSDDAIERNNKIYIPFSLDES
                     VLANKARVILKNGTTILLDKKDIKEDIDQEKGMKYTYFAVNGLEKGAIIEKLYVLEES
                     PEFTGQTIKMQDEYPIADLNFELIYPNYLVFKTKSYNKLSEPTIDEKKIQNTNVLSII
                     EKDIPALDNNEQYSNWALQLKLFRYKLDENLLSGGRNLNNFKEFSNNLYTRLNPELDK
                     KQQKAIDDFCATIPKSNDEQEQIWNIENKIKKTIGYDRYIDSKENLSDVIVTKQANQS
                     DILKFYSAVFKYFSIENNMVFTSNRYKIPFDKDFESFENLNELLFYFPTIKKYLTPTE
                     IEYRIPLFPNTIANNNGLFIKEKTFGGVKMGIGEINFIEIPGNDISHDIMNITIDFTQ
                     DIENPLITSNLTFGGYSGLNFQPIKDFASADQYKAVLKSIAENYTVETEYKTLTTEND
                     GIEFIGKKSFVLNVTFDGKELTKKAGENYLFSVGETIGKQMEFYQENKRTLPVEIDYP
                     HLYTRNIKIILPKGASVKNLDKFVMDYKTQVNGKTEAGFNSKYTKTGDEILIENTEFY
                     NIVNYPLNQFDDYKAVINAAADFNKIVIIVTK"
     gene            complement(26719..28509)
                     /gene="rho"
                     /locus_tag="PLQ70_00260"
     CDS             complement(26719..28509)
                     /gene="rho"
                     /locus_tag="PLQ70_00260"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQE33077.1"
                     /translation="MFDISALKEMKLSELQEIAKAAKTIKFNGVKKEVLINQILELQA
                     KTTDSNSGSKPAEPKEEVEKPKRTRIIAPKKVAIQKNATNSLFAKEEIAIEPKVEPVK
                     NEISVEVNPIEKKASKVIKFNKSAYEKKVALQKQKEEAKFASVGSESNEVSQANLEAE
                     TAENNEIEIPSKKINPNQLNKQKNNQNPNQNANPNQNGNANPNQNGNANPNFKNKKNN
                     FRDSDFEFDGIIESEGVLEMMPDGYGFLRSSDYNYLASPDDIYLSTSQIRLFGLKTGD
                     TVKGVVRPPKEGEKFFPLVRVLKINGHDPQVVRDRVSFEHLTPVFPSEKFKLAERQST
                     ISTRIIDLFSPIGKGQRGMIVAQPKTGKTMLLKDIANAIAANHPEVYLIVLLIDERPE
                     EVTDMIRSVRGEVIASTFDREPQEHVKIANIVLEKSKRLVECGHDVVILLDSITRLAR
                     AYNTVQPASGKVLSGGVDANALQKPKRFFGAARNVENGGSLSIIATALTETGSKMDEV
                     IFEEFKGTGNMELQLDRKIANKRIFPAIDLTSSSTRRDDLLLDAKTLQRMWIMRKYLS
                     DMNPVEAMDFINDRFKKTRNNEEFLISMND"
     gene            28689..29102
                     /locus_tag="PLQ70_00265"
     CDS             28689..29102
                     /locus_tag="PLQ70_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HQE33078.1"
                     /translation="MLQRIQTIYLLLAFVATGVLPFFLPLWTMSDGKDYLFMQNQVYV
                     VLLGLSTTLTIVSIVSYKKRQNQFVLGRLNIILNLILLGLFVYRSLNVSGETLAVSEK
                     GIGMFLPIVAIVLLVLANKAIKKDEDLVKSVDRLR"
     gene            complement(29235..29729)
                     /locus_tag="PLQ70_00270"
     CDS             complement(29235..29729)
                     /locus_tag="PLQ70_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQE33079.1"
                     /translation="MKKILLLSDTHSHIDDTILKYVEQADEVWHAGDIGDLVVTDTIK
                     KIKPLRGVYGNIDDDKARMEFSLHNRFMCEGVDVWITHIGGYPGKYNASIKAEIMTNP
                     PKLFICGHSHILKVMFDKKNNLLHMNPGAVGKSGFHQVRTMLRFVIDGDKIKDLEIVE
                     IGKK"
     gene            29960..30697
                     /gene="truA"
                     /locus_tag="PLQ70_00275"
     CDS             29960..30697
                     /gene="truA"
                     /locus_tag="PLQ70_00275"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQE33080.1"
                     /translation="MRYFIKLAYNGTHYHGWQCQPNASSVQETLNKAISVILNNEISL
                     MGAGRTDTGVHAKEMYAHFDFENPIDSSSLVHKLNSYLPKDIVIYDVILVHDEAHSRF
                     DAIKRTYEYHINTFKDAFLQDGSWYFHQKLDLDIMNQASKLLFNHIDFQCFSKVNTDV
                     NTFDCTIFEAYWKQENNQIIFTISANRFLRNMVRSIVGTLIYVGLHKITLEDFNSIIE
                     SKSREKAGFSVPAHGLYLTQITYDYLK"
     gene            30788..32545
                     /locus_tag="PLQ70_00280"
     CDS             30788..32545
                     /locus_tag="PLQ70_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE33081.1"
                     /translation="MKAKAFDTRLFKRILKYTKPYQFRFNGVIFFAIFLSVFAAVRPF
                     ILQGTVDTYIKPKDTYGLLMYVTLMGVTLVLEVVSQFFFVYWANWLGQDIVKDIRNKM
                     FKHILSFKMKYFDNVPVGQLVTRSVSDIEAIARIFSQGLFMIASDLMKMAVCLLIMFW
                     MNWQLSFIVVIAMPILVFFTRIFQRKMQVAFEEVRTQIANLNTFVQERVTGMKIVQLF
                     NREDIEFEKFKAINQKHNKAWIKTILYNSIFFPIADIISSLTLAFIVLYGGIQILNGS
                     TFTTLGQMTAYTMFIAMLFNPLRQIADKFNEMQLGMIAANRVFDILDTQDQIQDTGTV
                     EAPVFKGNIQFKEVHFGYIANEEVIKGIDLDVKAGQTIAIVGATGAGKSTIINLLNRF
                     YEINSGTICIDDHNIENYTLGSLRKQIAVVLQDVFLFADTIFNNITLNNPDISREQVL
                     AAAKKIGVHKFIMSLPDNYDFDVKERGVMLSSGQRQLIAFLRAYVSNPSILILDEATS
                     SIDTYSEELIQKATETITKGRTSIVIAHRLATILKADKIVVMDKGLIVEQGTHQELVE
                     KIGGYYKNLYDSQFVTEDN"
     gene            32585..>33079
                     /locus_tag="PLQ70_00285"
     CDS             32585..>33079
                     /locus_tag="PLQ70_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33082.1"
                     /translation="MKKFISYLIIIITYSCIPLKIAPSIEGEKITNAKKFKKDLPNFY
                     GFIFEDTKKANEFYNFINTKYNLKHINVESNVPIINNNNTYYLSFFERERTTKTLNLV
                     PIAIDADRNSKGKEPILEDLYTSRTGSWYLVLTLTDPEIKDCLNPNYPQREEIIKHLK
                     MVRDE"
BASE COUNT        11497 a         5646 c         5405 g        10521 t
ORIGIN      
        1 ttttcaatgg cggaatagct gcttcgatgc ttccgtcttc aaaataactc aaagccactt
       61 tttgttgagc ttcgacaata ttggcaatac catctacata atcttcgatt ccttgtagtt
      121 ctggtttcaa cattttttcg ttgaaaacgg cattgggttc atcgaatagg cgattcaaac
      181 atctcaaaga gaaagtactc gtaattctat accctaatct actggccaat actttcttgc
      241 cttggtactc gaaatcttct aatttttcta atgaaccatt ggccatcaaa acctttggat
      301 cacgatcttt agggtctaaa cgtgcccaga tttcaggaat caaaagactg atgtcgtgat
      361 ctactttatt ttctccacct acataacctg ctgcggtgct aaacgcattc gattcggtca
      421 aaatatgaga caacaaagca ttattcaaat cggatgttgg tgtcaacata ttaaatgggc
      481 ctttggtcaa agcgccttcc gatccagctc ctgttgttga tggtgattta cccgttaggc
      541 tgcaaataaa atccataaac aattcaggag tttcctgata atgaatgggg ttataaaccg
      601 ccaacggtcg aattcctgct ttcttgtcga ttggattgtt gcgtcttcct ggcaaaactg
      661 cattaactac attgataacg ggatcttctg attttatttt tcggtacaaa cgaactccaa
      721 tttcgcctaa ataactggct tcggtttcgt ctataaaaat attgcgctct aaataacgag
      781 ggtttttggt gggttcacca tctacaattc ttgtatgtga aggcagtgaa aaatattcct
      841 cttcattagg actgtttaca atccttagaa tcaaatcctt aaccggttgg gtgtatttgt
      901 cgaaattgat agtgtcttca aaaatttcga tagcatcttt ctttcttaac aattcatagt
      961 ttgtcaaaaa cacattgtct gatacaatat ccaattcagc acctttgtca taacctcgaa
     1021 caatagcttc gtcaggtctt tgaaacaaat gcgattcaca gttagcaaca actttcaaac
     1081 tttggttagt atattctgga tttaaatttt taatctgatg gcttggcaaa gtaatggatg
     1141 ccgagatatc atcttccatt tgaatttttt gactggcaga gaaatctgat cgcaacttat
     1201 tcaacaacca agttcctttt tgattgaaac caattcgaac ataacttccc acaactggag
     1261 tgttattgta ttgcaaacct gtaccgtttt tgccattgat gatttcaact gacatacaat
     1321 ctttccagtt caaagctccg tgatcctgac gatacaaacg ttttacaaac aacaccaacg
     1381 atcgaatgtg aaccggaatg tttttgataa actcattgta ttcatcagta tttctatctg
     1441 aaggagttag caattttact gccgaaccta gcgttcttcc tttatctaaa aacgatcttg
     1501 agggtggtaa tgagggatca gaatctttcc atcttttaga ataattgtat tcgattatct
     1561 catcagcttt tttgaaatct tcgtcaatat ctaaaatatt aaaagcacta taggtaatgg
     1621 cattttgcat cgatttagaa atttcggatt ttccaccacc cgaaacggta catggtttat
     1681 ggcaaaaaac accctctggc gaagtattta caattctcca aagcgcaatg gcattgtgtt
     1741 tctctaattt gaatttattt ccagtaggat gaatgtacgt tttatttgga gacaaaatta
     1801 gtttttgctc cttgccatca aaagtccaag taatactatt agtattggta ttgatatagg
     1861 cattctctgg aatataaact aagtttggat atttcttatc tactgcatag tttattggtt
     1921 tcacctcaat tctatcgcct agaaaagctt taacaccttc aaaattatat tgattcccga
     1981 acttttcaga aaaagcactt ccatcaacaa tatcgcccat tacacctcta ggatatgcga
     2041 tcgctccacc cgaatgttct tcctcaacca aaccaaataa attagccgaa taactaattt
     2101 gagttttaat ttctttttta gaatatccaa aataattatc ggcaataatg gtgaccacca
     2161 ctcctctatc gtctctacaa gttactttaa aagcgccacc gttgttatac aattcgttct
     2221 cgtctttcca gcacattcct tccttttttt gacgctctgt ggcatcatca aaatgaggca
     2281 aaccaatgtc ttttttcttc aacaaacgca attgtggagc cagaatcaca cagcctgtat
     2341 ggcctgtcca atgttctgtg tctaatgcag catcattttg agctagataa ggatcgcctg
     2401 cgtttccaaa aatagcttca acgaaatcaa ggttactcac caaacttcct ggtgcaaaaa
     2461 agcgaacttc catcgacttt ttagaaataa ctccttttac ttctggacaa accatagggc
     2521 gcatcaacaa cgaaaccatc aatttggctt tttcttcttg attggatgta aatggcaata
     2581 atttcagttt gtccgaaggg ttgaaagcag cattcaaaaa ataggcgaaa gcaattttag
     2641 gaacttcttt tttatctaaa ggtgctggca aattgccttc tacaatatga aaagtaccta
     2701 tcgtggttcg cttgtcattt actggattat ttaaaatccc ttgcctgatg cgataagaag
     2761 taatatcatc acttgcaaaa gtggttccgt taggtggcaa actcaattct cttgcatgac
     2821 cactttgggt taaaatcaaa gtatcatttg gtaaaacata aggtttattg aaaggtacac
     2881 ctttgagata atcgttcaaa aagttttgaa ttctgttgtc ggctggcgaa agatgattgg
     2941 ataataatct cgatttttcg cgaaaacttt tgattaatcc attagtcaat gtttggaatt
     3001 tgggattgca aaaaggctct gtcgaattct catcatctcg ataaatgggt tgtccaagtg
     3061 atgccaattg aagattgatg tattgaacag tatcttttct gctttgctta attttttcct
     3121 gatttacttt attgtctgta gtcatttata aaaatagtat ttgtcaatgt gtgttgctgt
     3181 ctgtataaat aataaattat tattttttat ttcgtacaaa ggtggcgaaa accgacttaa
     3241 aattaaagaa aaaaagaggc tatttttcaa agaaatacga tttgtgaaat cgattacact
     3301 aaaacgattt cacaaccaaa aaatttgata aaaacataac aaatagtatt atatttgcgt
     3361 tttttaaaac atttttaagt atgaaaaaat tattgccact attactcctt ttcacccttt
     3421 ttatttcttg ctctaaagac gatgctcaaa ttgttgatta taccgctcaa aacgaaaaag
     3481 aaattaccga ttttatagca ctcaataaat taaacgctca aagaagtgct agtggtttgt
     3541 attatgtgat taataatccc ggaactggaa cccaacccac tgcaacttca aatgtaacgg
     3601 ttgcttacaa aggtacattt accaacggaa caatatttga ccaaagcacc aatgctggaa
     3661 tttcaattgg attaaatcaa gttattaaag gttggaccga agggattcaa catttcaaac
     3721 cgggaggaag tggtttgctt ttgattccag cccacttggg ttatggaagt tataactatc
     3781 aaggtattcc tggaggttcg gtactaattt tcgaaattag tttaatttca gtaaatcaat
     3841 aaagatcgtt tttatacctt cttctaaatt ataataaaat gccacaacag aatttatttt
     3901 gttgtggtat ttttttgtct aaataaatcc attttgatta aaaagcagcc ctaaaattta
     3961 ttttggcaca aaacgaaatc attaaaaaaa ttttctattt ttataccttt taaaaatgaa
     4021 ttgattgaaa aaacctgcca aactattttg gataaaacat agagcttcaa atggatacta
     4081 aaactattct tgaagcaaaa tctatttgtt tcttgctcat tttcactttc tattttcagc
     4141 ttagcttttc acaagaagaa ggtgaaaaaa aacaacccct acgcacgatt cttgatttgt
     4201 ttactaaaga agacactctt aaaattaaac taccaaaaac caaaaccaaa ttaattgcct
     4261 tccctacttt gggctatcaa cccgctaatg gttttacact tggattcatt agccaatata
     4321 gttttaaaga aaaagaagaa aacaaaattt cgctcatttc gggtggcgct tctttaagta
     4381 caaaaaagca actcctcacc tatttaaaaa actatatgta ccttgatgat gaccgctatt
     4441 tttttagtgg tgattttcgt tattacattt tttcgcaatc caattatgga ttaggaactg
     4501 acattgttcc ctggggagcc gaattcaaag actttgatta caagtcaatt gaacaaccaa
     4561 tgaattataa ttacttcaaa tttcacgaaa caatctcttt caatatcttt ccttcttttt
     4621 ttattggcac aggaattcat atcgacagtt ataacaacat taatgataaa cttttggatg
     4681 tctcccaaga agaatacact tatcattata attattcaaa gaaatatggc tttagcaaca
     4741 agaattatgc agtcaatggt ttgagcatga atttaattta tgacactcga gacaacctca
     4801 taaacacaaa caacggtcta tatttgaatt taaattaccg ttataatcca aaactattta
     4861 aaggtcaaca aaaaagttcc tccctcttgc tcgaaagccg ctattttatt cccatcagta
     4921 aacggaataa acagcacgtt attggttttt ggggctatag tcaattcgta accagcggac
     4981 atttgcctta tttaaaccta cccgctatag gttgggatca aaatagtcga agcggaaaag
     5041 gatacataca aggccttttt agaggcaata atttgatatt tcttgaaagc gaataccgtt
     5101 ttcccatcac caaaaatcaa atgattagcg gaaccgtatt tgctaatgcc acaacagcca
     5161 gcgatgcaaa caaaaacctc cgtttattcg aatccattca gccagcagtt ggcattggtt
     5221 tgcgattctt gttggacaaa aacacgctga ccaattttgt agttaattat ggactaggcc
     5281 gagattccca aacgttctat tttaatgatg gcgaggggtt ttaaattata aatcagcccc
     5341 tgcattattt ttattatggc gtgtccctac gggtcgggct gtacgttccc gcttcccgat
     5401 aaaaaatcgg gagagctcct ctgccatccc tcacgcaaaa aaacacccca aataaatact
     5461 attttccgtg aaattattgc tattattttc tatatttgga aaaactaata aaccgacaaa
     5521 acttagccaa tctacccgat tttgtgtgta tatactaagg tttgtagagg atataaatga
     5581 gttgggtgaa atgttaaaga ccgatgattg acgaatttta aattaaaaac attacttttg
     5641 attataaaat atttataaaa aatatggaaa ttgaaataaa gaattttgga ccaattgata
     5701 gtttaaaatt caatttaaat aaagatttac acctaatata cggtcaaaat gcaattggta
     5761 aatcatatgc aacatattgc ctatattgtt taatcaaaaa cattaaaaat aaatccattg
     5821 gcaatagaca tatgtacttt tatgataaac gtgatgaaaa tgcatatgaa aaattcataa
     5881 aaagtaaaat aaaaagctta aaaactaata aatcaataga ttgcacaaaa gagtttaata
     5941 cacttattga aattgaatta aaagagatta ttttaaaagg attacaaaat tcactattga
     6001 atactttttc ttctttaaaa aatttaaaga atagatactc tgataaaaat tatgaattaa
     6061 ttatatctat ttcaaaatat gaaaaattat caatactttc taatgatgaa ggtgaattag
     6121 atttaaaata tgagaatgaa ataaaaaaaa tagaattatt tcaaaaagaa acaaagtcta
     6181 caaaatattc attaattctt aataatcgca aagaatttgg taaaccaacc gaagatgagt
     6241 tcattcacga atttataaga ttttcatacg taaaaatcaa ccaaatatta agaaaattag
     6301 attttaatat caaagatgta tattatctac ccgctagtag atctggactt taccaagctt
     6361 taagtgcgtt tactccaatc attgcggagc taacacaaaa tagattcttt attcaaaata
     6421 aaagcattga attaccatca ctctcagaac ctttagctga ctattttata gatttatcca
     6481 ctgtagataa aaaacataac aataaagagt ttgaaggtat tgttaaatta ttacagaatg
     6541 aaatccttan nnnnnnnnna aaagtagatt ataatgatga aacaagaagg atttcatatc
     6601 aacctgatgg tattgattta gaactacatt tatcagaagc ctcttcaatg gtttcagaat
     6661 tatctccttt agtattgtat tttaagcata ttttaaataa caaatattct tcaataaata
     6721 gtagggatag ttttttcttc gatccttttt ataacgattc atttggaaag agaaaaaaca
     6781 aaggatatga tattttattt attgaagaac ccgaagcaca tctacatcca gaagttcaag
     6841 taaaattaat tgaaatattt gcaaaactaa caggtttaaa tttgaaaata tttattactt
     6901 ctcatagtaa ttatatgttc aataaaatca ataatatttt aataaaaaaa gaaattgaaa
     6961 aagaaaaagt tatggtttat catttaatta aaactggaaa tggaactaca acaaatattg
     7021 aaatgaaagt aactgaagat ggtattaacg attcaaattt ccaagatgtt tctgagcaat
     7081 tatattatga acgtttaaat tatttggaga agaatgatta ataatattat ttctcacgat
     7141 actctatctc aatatataga agatacttgt tgcgaaaatg gtgtttgtgt atcttttgaa
     7201 gacgatatag atcctaattc ttatgtaatt attaaagttg atcaattcta caactcttta
     7261 aatatagaga aaaggccagc atctattgat tgtctaataa ttcgaaaatg tcttattagt
     7321 ggctatggtt taacattagt tgaattaaag aatattgaaa attccaaagg atttgattta
     7381 gaaaacatga aatcaaaatt tgatacaact ttaaatgatt ttataaaaaa tagatttaga
     7441 gatcctttag atatagatta taaggacata aaactttatt ttgtttcaaa acaagagatt
     7501 tatagaaggg atttaggtct taaaatggaa gttctgatga atgtaagatt taagtttaat
     7561 gaaaaacaac ttatgataat cccaagaatg ccaaatccta caattaagaa atgttactaa
     7621 aaaaaaacac ttcgcccaac acctcgagtt tcgttgcgtg tgcagcacga acaatgaaac
     7681 tcctgctgaa cccttcaata aactcaggac aggctcacct attacaagtc cgtcaacact
     7741 atggagttat tccaacaaat aaaaaccaca acaaaattaa attctgttgt ggtttttcgt
     7801 ttaaaaaata ggctgctacc aagcaatcgg acgatagtct ttcaaaaact ttccactcca
     7861 atgttttcct gaattgattc cgtcaaacaa cggatccatc actctcgctg ctccatctac
     7921 aatatccaat ggcggttgaa aatcttgttc ttcttgtttt cgtttagcta attcggcagg
     7981 atcttcatct gttacccaac ctgtatctac ggcattcatg aaaattccat ctttggccaa
     8041 agttcctgca gcagtatgcg tcaacatatt caaagcggct ttcgccatat tggtatgcgg
     8101 atggcggtct tctttgaaat cacgataaaa cttcccttcc attgccgaaa cattgatgat
     8161 gtgttttttg ccagtatgat cctttttcat cacttccgaa aggcggttgc acaacacgaa
     8221 aggtgccacc gaattgacca attgcacttc gatcatttcg gtagtttcta tttgtcctaa
     8281 ctttaatcgc cagctgttta cttttcgtaa atcgacttgt tgcaaatcgg catcgagttc
     8341 gccttctgga aaaacttcct gcgccaccaa agcattatca aacgaatatg gaatttgtga
     8401 taatttggcg gaagcccgca aaccaattcc aggttctggt ccgtgccaag ttactggcat
     8461 attttcattc gacgatgcgc ctaaagtcaa aactttcaac tcctccaaac aattggtgtg
     8521 atccaataat aattcttgtg ctttttgggg caaagaagca ataggacgct cttcattttc
     8581 tatcaaatgg gtataaaaac cagcaggtcg tcgcacagtt tgagcagcat tattgattaa
     8641 aatatccaat cgaccgtatt tttgctcaat gaaattacaa aaaatctcca cactcggaat
     8701 gtgccttaaa tccaatccgt gaattttcaa acgatgtccc cattcgttaa aatcgtcttc
     8761 tttagaaaat cgcaaagccg aatcgacagg aaaacgagtc gtagcaatga cagttgctcc
     8821 accacgcaaa agcatcaagg taatatgata accaattttc aggcgagaac ctgtaatcac
     8881 ggctatttgc ccattaacat cggcggtttg aaaacgtttg gcataattaa aatcgccaca
     8941 atcgcagcac attgtatcat aaaaatggtg cattttggta aattctgttt tacagacata
     9001 gcactttttg ggcgtttcga gttccaattg ttctttgtgt tccaaatcgt gcgaagccaa
     9061 taatttgggt gctacaaaaa cactcgcttc ccgagccgaa cgaattccgg tttccttgcg
     9121 agccgttcga tctttttttt cctgtttgcg tttggcaact gcttttccgt ctttttttct
     9181 cttggcaaat tcatctctat caggacgtga aaattgtcct gccgctttga ttaaagcaat
     9241 tcgttgctct tttggaattt cgaatatttg gtcagtatcg gtatttaatt gtgccaaaat
     9301 agcaatgcat cggtcaattt cttccgaact tattgtagtt ttattttcta tttcttcgtt
     9361 taccattgta aaaaagcatt ttctttagga aaattacaag gctgcaaata tagtgtttaa
     9421 agttatattg ttttcaaagt caaaagctat ttcatagtga ctctcaaagc aaacgcaaag
     9481 cctcacaaag tttatttttt gtttttcaaa ttttaaaaaa tctccatgta tctttgtgtt
     9541 tttagtttgc gaacctttgc gtaataaaat atgttattcc aaatccagtc ttatcttaaa
     9601 ttcctttggc attccaaaaa cgaacacgct gtgcattcgc catttgtttt taatttgata
     9661 accaaatgct tttatgacaa aaaatcaaaa ccacaatatg cagttttaaa agaatataga
     9721 aattcgcttt tagcaaacaa aaacacgatt gaagttaccg attttggtgc tggttctaaa
     9781 gttttcaaat ccaatactag gcaaattaat aagattgcca aaaccgctgg aatctcgcca
     9841 aaacgtgctg aattattgtt tagaattaca aattatttca aacctaaaaa cattttagaa
     9901 attggcactt cattaggatt agcaacctct gccctcgcct taggatcccg aagcgccggg
     9961 acaaaagcaa acattatcac attggaaggt tgcccaaata cagcaaatca atgtcaattg
    10021 caattgcaaa aattcaatat caatactatc gatttagttg taacagaatt cacaagttat
    10081 ttgaacaact gccaactaaa aactgaccac tgccaactga tttatttcga tggcaaccat
    10141 tccaaaacct ccactttaga gtattttgaa ttgcttttac caaccataac caatgactct
    10201 gtttggattt tcgatgacat tcattggtct cccgatatgg aagaagcttg ggaaattata
    10261 aaaaaacatc caaaagtaac cgtgactatc gacacttttc aatggggatt gattttcttc
    10321 agacaagaac aaccgaagga acattttgtg attcgggttt aaaatagttt aaaagtttaa
    10381 gggtttaaga gattaaagtt atttaaattt gttgtaacat tactaaacat tagtagtcct
    10441 atttggcatt aaaccatttt aaactcttta actataaacc aataaatggc aaacccactc
    10501 ataaaaatag aaagcatcaa acgagatttt gctctaggaa acgaaatcgt ttatgtgctt
    10561 aaaggaattg atttagaaat agaaaaaggc gaatatgtag ctttgatggg gccttctggt
    10621 tctggaaaat caactttaat gaatcttttg ggatgcttgg acacacccac ttctggaagt
    10681 tatatcctta acggaaaaga tgtgagccaa atgcaagatg atgaattggc cgatattcgc
    10741 aacaaggaaa ttggttttat ttttcaaacc ttcaaccttt tgccaagaac aactgcgctt
    10801 gataatgtgg ccttgcccat gatttatgct gggcattcta aatcggaacg aaacattcga
    10861 gcaaccgaag ttttaaaaca agtgaatcta gccgacagaa tggaccatca acccaaccaa
    10921 ctttcgggag gtcaaagaca aagagttgca attgcgagag ctttagtcaa caaaccttcc
    10981 atcattcttg ccgatgaacc cactggaaat cttgacagta aaacatcaga agaaattatg
    11041 gcacttttag aagaaattca tcaaaaaggc aacactatta ttgtagtaac ccacgaagaa
    11101 gacattgctg ctcacgccaa aagaatcatc agaatgcgtg atggaatgat tgaaagcgac
    11161 acttctaaat aattttagat tgcagatttt agactgcaga ttgcaaatct aaaatcagaa
    11221 ctctaaaatc taaaatcctt aaaatgaaaa tttatactaa aaccggcgac aaaggaacta
    11281 ccgcactttt tggcggaact cgggttccta aagaccatat tcgcattgaa agttacggca
    11341 ccgttgatga attaaattcc tatattggat taatccgcga ccaagaaatc aattcacatt
    11401 acaaaaacat actcattgaa gttcaggatc gattgtttac cgttggtgcc attttggcta
    11461 ctccacccga aaaagaagtg aagaaaaacg gccaattgcg cttgcaaaaa ctcggaatta
    11521 tagacagcga tatcgaatta ctagaaaacg aaatcgatgt tatggaagaa tcgcttcagc
    11581 caatgactca ttttgtttta cctggtggac atactactgt gtcatattgt cacattgctc
    11641 gttgtgtttg ccgtcgtgcc gaacgaatag cagtgcattt agaccataat gaacccgttt
    11701 ccgaaatcgt aattaggtac ttaaaccgac tttctgacta cctttttgtc ttggcacgaa
    11761 agttgtcgag tgatttaaac gctgaggaag taaaatggat tccaagaaaa taatattttc
    11821 taaattcatc taaaaaataa caaaatatta aatcttttta agaagaactt caataaattc
    11881 ggcttttaaa aaatcaaaaa aacacgttct taaatttaat taaaaataaa tgatttttta
    11941 cttgtatttt tcagtaaaaa atttattttt gcacaaaact aaacagacat aagatgtatt
    12001 ggacattata attagcatct tatttaagtg atgcaccgtg gcctgctaac aaagacgaac
    12061 ttattgacta cgctattaga gctggagctc cattagaagt agtagaaaac cttcaatcta
    12121 ttgaagatga gggcgagata tatgaatcaa tggaagaaat ttggccagat tatcctactg
    12181 acgaagatta tctttggaat gaggatgaat attaaaaaat atctcaagaa aaaagtctcg
    12241 tatgaggctt tttttttggt taaatttacg cacttttata aatagaaata aaaacacatt
    12301 atgagtttca taaacagtat tatcaaagcc tttgtaggtg ataaatctca aaaagatgtc
    12361 aaggctttgc aaccttattt agccaaaatt aaaactttcg aaactgcttt ggccgcttta
    12421 tcaaatgatg acttaagagc tagaacaact tcttttaaag aaaaaataaa acaagctcga
    12481 gccgataaag atgctaaaat cgcaacactg cagctacaag ccgaaaatac agaagatatc
    12541 gacaaaagag aagatattta tgtagaaatt gatgctttag aaaaagaagc ttacgaaatt
    12601 tcggaaaaaa ccttaatgga aattcttcct gaagcttttg ctgtggttaa agaaactgcc
    12661 aaacgtttta aagaaaacac tcaaatacaa gtcactgcaa caccattaga tcgagaactt
    12721 tcggcaacga aatcgtatat aactcttgat ggtgaaaatg ctatttgggc caactcttgg
    12781 agtgctgctg gtaaagcaat tacttgggat atgattcatt atgatgtcca actaattggc
    12841 ggaatggttt tgcacgaagg taaaattgcc gaaatgcaaa caggtgaagg aaaaacatta
    12901 gtagcgactt tgcctattta tctcaacgct ttgactggaa acggagtgca tttggtaact
    12961 gtaaatgatt acttggccaa acgtgatagt acttggaaag cgcctctttt cgaatttcac
    13021 ggtttgaccg ttgattgtat agacaaccac caacccaatt cagaaggtag aaaaaaagcc
    13081 tataatgccg atattaccta tggtacaaat aatgaatttg gtttcgatta tctgagagac
    13141 aatatggcgc attcgccaga tgatttggtg caacgcaaac acaattatgc cattgtcgat
    13201 gaggtcgatt cggttttgat tgatgatgcg agaacaccat tgattatttc tggtccagtt
    13261 ccagaaggtg atcgtcacga atttaatgag ttgaaaccaa aaatcgaaaa tttggttgcc
    13321 ctacagcgtc aattggccaa cggatttttg tcggaagcaa aaaaattaat caaagaaggc
    13381 aatactaaag atggtggttt cttattattg agagcgcacc gaagcttgcc taaaaacaaa
    13441 gcattaatca aatttttgag tgaagaagga atcaaacaat tgcttcaaaa aaccgaaaac
    13501 caatacatgc aagacaacaa tcgcgaaatg ccaaaagtag atgaagcatt gtattttgtg
    13561 attgaagaaa aaaacaatca ggtagaattg accgataacg gaatcaaatt cctttcagga
    13621 gataccgaca gcaatttctt cgttttacca gacattggaa cggaaatagc cattatcgaa
    13681 aagcaaaatc ttgaaaaaga caaagaagcc gaagagaaag agaaattatt ccaagatttt
    13741 ggtgtaaaaa gcgagcgtat tcacactttg actcaacttt tgaaagccta tacgcttttc
    13801 gaaaaagatg tcgaatatgt aattatggac aataaaatta tgattgtcga tgagcaaacg
    13861 ggtcgtatta tggatggtcg tcgttattca gacggactac accaagctat tgaggctaaa
    13921 gaaagtgtaa aaattgaaga tgctactcaa acatttgcaa cagtaacact tcaaaactat
    13981 ttcagaatgt acagcaaatt aggcggtatg actggaactg cagtaactga agctggtgag
    14041 ttatggcaaa tctataaatt ggatgttgta gagattccta ctaacaaacc aatgttgcga
    14101 aaagacaaag aagattttat ttataaaact actcgtgaaa aattcaatgc tgtaattgac
    14161 gatgttacag aattatccaa ctcaggaaga cccgttttga ttggaacaac atcagtagaa
    14221 atttcggaat tattgagcag aatgctaaaa atgcgtggta ttacccacaa cgttttgaat
    14281 gccaaaatgc acaaacaaga agcccaaatt gttgaagaag caggaaaagc tggagtagta
    14341 actattgcaa ccaatatggc tggtcgtgga accgatatta aattgactcc cgaagtaaaa
    14401 gccgctggag gtttagccat tgttggtact gagcgtcacg attcgcgtcg tgtggatcgt
    14461 cagttgcgtg gtcgtgctgg tcgccaagga gatccaggaa gttcacaatt ttatgtttcg
    14521 cttgaagaca acttgatgcg tttgtttggt tctgaaagag tggccaaagt aatggaccga
    14581 atgggattgc aagaaggtga agtaattcaa cattcgatga tgactaaatc tatcgaaaga
    14641 gcacagaaaa aagtagaaga aaacaatttt ggagttcgta aacgtttatt agaatatgat
    14701 gacgtaatga atgcgcaacg tgaagtggtt tacaaacgcc gtcgtcacgc tttgcacgga
    14761 gaacgtttga aactcgatat cgccaatatg ttgtatgaca cttgcgattt aattgtaact
    14821 gagaataaat taactcatga tttcaagaat tttgaattcg aattgattcg ttatttctct
    14881 atcacttctc cagtttctga aagtgatttc aataaattga ccgacataga attgactgga
    14941 aaagtatata aagccgcttc tcaattctat gcagaaaaaa ctgaacgaag tgccagagaa
    15001 gcatttccta tcatcagtaa tgtttatgaa gataaaaaca accaatacga acgcatcatt
    15061 gttccattta ctgatggaat aaaatcgcta aatgtggtta ctgatttgaa aaaagcatat
    15121 gaaacaaacg gaaatcaatt ggttgctgat tttgagaaaa acatcacttt ggccattgta
    15181 gatgaagctt ggaaaaaaca tttacgcaaa atggacgaat tgaaacaatc ggttcaacta
    15241 gcagttcacg aacaaaaaga tcctttgctt atttataaat tcgaagccta caatttgttt
    15301 agttctatgc taaatggcgt gaacaaagaa gtgatttcgt tcttgtttaa aggtgatttg
    15361 ccccaacaac aagctccagc gattcaggaa gcaaaacagg ttcgtcaaaa agaaaactac
    15421 acagaaagta aagacgaaat tgttagcagc gaaagcgcca atcgtgaagc gggacaaacc
    15481 caacaacgac aagtgacaga gactattgtt cgtgatcaac cgaaaattaa tcgcaacgat
    15541 accgtaacca ttcaaaatgt tgccaacgga caaatacaag aaatgaaata caaaaaagcc
    15601 gaaagtttga ttgctaacgg aacttgggtt atcgtttcat aattagaaaa taaatttaat
    15661 ttcatcccca attgctaaaa agtaattggg gatttttttt tacatttgat acataataaa
    15721 acctctaaaa taaaacccca aatgaaaaac cttatcgcaa gcataacttt actattaatt
    15781 acaactacta gtctttcagc ccaaactaac accaaagaat ataaaattgg acatgttgtc
    15841 aatgtcagct tacccgatta tatgactaga acaatgggct taaatagcgc agcaatgttt
    15901 cagtataaaa atacaattaa agatgtttat gggtttatta ttgaagacaa taaagaagaa
    15961 ttagccttag cagaaatgaa gtactcctct ataaatgaat tttgcgagga atttgccaaa
    16021 gattttttga aagatgaaaa gaaaaaaact ttttcgtatc cagaatatca aaaaaaagga
    16081 gacatcagtt ttgcggaatt tgacgcaacc tattatgata aagacatcaa aggagaagta
    16141 tattatttgg ttggaattgt tgaaacaaaa accgcttatt ataaagttat atcattcaca
    16201 agcaaagcca ataaagataa atttaaagcc gattttcaaa gtatccttta cagcgtaaac
    16261 gattaatgtg tctcctgttt tcataaaaaa tcattaaatc taattcatga aaaagggcta
    16321 aaacccataa attttgaaaa aaatcactat atttacttga ttaaaaaact aataagtaaa
    16381 acctaaaaat aaattatcat ggcaaaaagt caagatgcta agaaaaatgt aaaaaaagag
    16441 cctcttaaaa ccgcaaagga aaaggcagcc gaaaaaagag aaaagaaaaa tagcccaaaa
    16501 agggattagt cagcagttca cagattgcag aattcagaaa aaagcagttt aaaatgaaat
    16561 gaatcatttt taaactgctt ttattatttc tgaaaaatgg tttgatttct atgtacagaa
    16621 atctgtaacc taaaatctga ttactttgtc aaaggaatgt tctctaaaat ttcaataaca
    16681 aatttccaga atttttgtga agatttaatc gaagctcttt cgtctggcga atgtgctccc
    16741 tgaatcgttg gtccaaaaga aatcatatcc atatcaggat aatttgtacc aagaattcca
    16801 cattctaaac cagcgtgaca agctactact tttggttttt cgttgttttg tttttcgtaa
    16861 attttgacca ataaatccaa aatttcggac ttcacattgg gagtccaacc cggataagaa
    16921 ccagccaaag ttacttcgca accgcacaat tcaaatgcag aacgcaaagc attcgccaaa
    16981 tcaaatttag aagtttcaac cgaagaacgc gtcaaacagc taatagaaat ttcaccatct
    17041 ttgatgataa cgcgagccac attattcgaa gtctctacca aatcttccat atcggccgac
    17101 attctgtaaa caccattgtg agcggcataa atcgaacgaa gaatcccttc ctgaacgccc
    17161 aaatccataa cttttttagg caaatcgcat ttcacgattt cgatggtcaa gttaggttcg
    17221 gtagttttat attccgtttt gatgtcgctg atgatttcct gcatatcaaa aacataagct
    17281 tcatcataca ttccagcaac aattactttg gctacacttt ctctcggaat ggcattgcgt
    17341 aaacttcctc cgttgatttc agcaacttgt aggccaaaat tttcgaaagc gtcgaacaac
    17401 aaacgattca ttattttatt ggcattaccc aaacctttgt gaatatccat tcctgaatgt
    17461 cctccattca agccttttac agtaatggtg tacccaacag aatcttcagg agtttcctct
    17521 tcttgataac ttctagtcgc tgtaacatcg attcctcctg cgcaaccaat gtcaatttca
    17581 tcgtcttctt cagtatccat attcaaaaga atttctcctt tcaggatgcc accttttagg
    17641 ttcaaagcgc ctgtcattcc agtttcttca tcaatggtaa acaaagcttc aatggcagga
    17701 tgtgcaattt ctttggattc taaaattgcc attatcattg ccactcccaa tccgttatca
    17761 gcgccaaggg tagttccacg agcccgaacc cagtcgccat cgacatacat atcaattcct
    17821 tgcgtatcaa aatcaaaatt ggtgtcggca tttttttgat gtaccatatc caaatggcct
    17881 tgcagcacaa cggttttacg attttccatt ccagcagtgg caggtttgcg gataatcacg
    17941 tttcggattt cgtcttcaaa ggtttctaat cccaatgaat ttccaaagtt tttcataaac
    18001 tcgattactc gttcttcttt ttttgatgga cgaggaacgg cgttcaaatc ggcaaatttg
    18061 ttccaaagtg ctttgggttc taggtttcta atttcttggc tcatttattt ttgtgtgagg
    18121 tttaacattt acaaagctca aagttacaaa tattaaaaac tttttgaata ctaattgata
    18181 ttgtaattat atttacattc aaaatatttt cagtgcaacg caaatacatc cttcccctat
    18241 tcctcctaat tcaaattcta tttctgaaaa taattgcctt ttttcctgga tgggtagaac
    18301 gcttttacag caatggtttg tatgttttga tttcgaaatt ttcacggata gttttaggga
    18361 taataccttt ttctgtcgga gatattatgt atggaattct gattttttat cttttaaagt
    18421 cgatttggaa agcaagaaaa acttggaaaa cagaatggaa aaataatctt ttaaaagttt
    18481 tgaatgtcct ttcggttgcg tattttctgt ttcatttgtt gtgggctttg aattattacc
    18541 gtcaaccgct gttcgaaaaa atgcaaatcg aaagagatta taacaatgct gatttattcg
    18601 attttactaa aaaactgatt gccaaaacca atcaaattca aagtcagatt accaaaaatg
    18661 atagcaagaa agtagttttt ccttattcgc aaaaacaagt ttttgagatg aatttgaatg
    18721 gatataaaac gctttcaaac gaataccact tttttgaata tactaatcct agtatcaaga
    18781 aatcactttt tagtttgcca ttgacttata tgggttttgg tggttattta aacccgttta
    18841 ccaatgaagc tcaagtcaat tatttgggac caatgtatgg ttttcctatg acaacaaacc
    18901 acgaaatggc acaccaaatg ggatttgcca gcgaaagtga atgcaatttc atcggatttc
    18961 tggcatctgt gaaaaacgaa aatttataca ttcaatattc gggatatagc aatgctttgc
    19021 gttattgttt gggcaattgg tatgttcgag acgaaaaagt gtatgatcaa ttattgaaaa
    19081 cgattcatcc cggaattttg aaaaactatc aggaaaacga agatttttgg aaacaatatg
    19141 aaacaccaat cgaaactggt tttcatattt tctatgacaa tttcttgaaa ataaaccagc
    19201 aaaaagacgg aatggatagt tatagcaaat ttgtgaattt gatggtgaat tattataaag
    19261 gaaagaaatt ataaaagatg gaacgctaat gacacagatt taagcggata ttttttcgtt
    19321 tatcaactaa cttgtaatta acttcaggat ttgaatatat gttcaaatct tgaacacctg
    19381 atttaacgca ttaaatttgt aacaaaatct tctttttgac cactaactta aatatgagcg
    19441 acgatttaca acaatctttt gtgcttcaat tgaagaccaa tcagaatata atccacaaga
    19501 tttgtaggtt atatacgaat ggtgaggatg cgcataagga tttgtttcag gaaatcacga
    19561 ttcagttgtg gaaagccttt ccgaagtttc gtggcgacag caaattttcg acttgggcct
    19621 atcgagtcgc cttgaatacg gccattactt tataccgaaa aagcactcgc tcagtcaaaa
    19681 cggtagatta tgaaactcaa caatatttta ttagcaatga agattataat tatgaagaag
    19741 aagaacgatt aaaattaatg tacaaggctg tttaccaact gaatgatatt gaaaaagcct
    19801 tgatttttat gtatcttgaa gacaaggatt atcaagaaat ttcagaaact ttgggaatta
    19861 gcgaagtgaa tgctagggtg aaaatgaata gaattaaagg gaaattaaaa aaaatactaa
    19921 atccataaca ttatgaaaga gctggattta ttaaaaagag attggcacaa aaacaatgcc
    19981 ttcgaacagg tttctgaagt agaaatttat aagatgttgc accaaaaatc gtcttcgatt
    20041 gtgaagtgga ttttgattgt gagccttata gaatttgtag taataaatgg tatttcatta
    20101 ttactcaatg atccaaaata cgatgaattt ataagcaatc acccttatat caattattta
    20161 gagaaattta attatgttgt aattgtagct tttatctatt tgttttatag aaatttcaaa
    20221 aacatttcgg tcttgaattc ttcaaaaaca ttgataaaac acatcctaaa aaccagaaaa
    20281 attgttactt actatattta ctggaacatt tttataggcg gaattacagg agctttgagt
    20341 ggaattgaag gtttcaacga aggttttaaa tctggaggag gagataacgc tgttttagcc
    20401 acaaattgcc ttacaataat tattatggct gcattgataa tgggctttat ctggttgttt
    20461 tacaaactat tatatggcag tttcctacgc aaacttaaag ataattatac cgaattaaaa
    20521 aaaatagatc tataattcgg aatttctttt taataatccc attgtcttaa tttgttcaat
    20581 tcctcagctg cttttaattc ttcggcagga attactttca aaaaggaagg atgttgctcg
    20641 atagcatact caattttttc aacaatttgt tcgatggtat cgttgtcata atcaatttcc
    20701 attggtggtt tgatgatgaa agattgaaga atgtctttct ttttcattcg caatcctttt
    20761 ttatcaaacg aacgtcggaa accgtcaatc acaatgggaa ctacaattgg tcgatgctgt
    20821 ttgatgatat gtgcagttcc ttttcgaacg ggtttaaatg attttgtggt gccttgcgga
    20881 aaagtaatta cccaaccatc attcaatgcg attttaatat tttcggtatc attaggattt
    20941 acatctcttt tttcggtaac gtcttttcca caagaacgcc aagttctttc tactgtaatt
    21001 gctcctatat aagctaaaat tcggggtaat aatccggctt gcatagtttc tttggcagct
    21061 acataataaa tattcaactt gggtttccaa atgtatccaa tattttttat tgaatcttga
    21121 cgaccgctca aactggcatt aaaaacgtga aacatggcaa ccacatcagc aaaataggtt
    21181 tgatgattgg atataaaaag tacattcgca tcaggtaatt tggtaattat ttctgatcct
    21241 tctatttgca aactattaaa accacgatag cgtctgtgag ttaaaaccgc aaaaacgtgt
    21301 attaaaaatc ttttaacaac aagaatatgt ccaaaaggat ttctcttaaa tagcgccata
    21361 aaatagtgtg taataaataa attagaaagg caaacataca aaaacttata atttatagca
    21421 cttttttcag gacttctttc aactcgctca gcatcatcgc tgtagcaccc caaacgatat
    21481 gctcgttgat tttgtatgca ggaatttcaa tatctttggc gtaagacgtt gtcattatag
    21541 cacttacaat taaattatca ttcaaaaaat cagataatgg caattctata atttgagcaa
    21601 cttcattact atctggaata aaaacaattt cttctttgct gattcctaaa aaaggataca
    21661 ccataaaatt actggcttga atgtacaaat tcgtaaaagg cataataatt tcgatttgct
    21721 caggatgaat gccaatttct tcttgagttt cacgcaaagc tgtatttaca aaggtttggt
    21781 cgttggattc atattttcct cctggaaaag caatttgggc tgaatgaatg ccttcgtaag
    21841 catttcgaac aataagcacc aaatgagttc ttcctttttt gggataaaac aacatcatta
    21901 cagcggcagt tttggggtta aaactgtcta agttttgatt tttgaggctt tcaatccgtt
    21961 ccaatggcgc cattttgaaa tgtgcttctt tcgctggcaa atttacattt actaattttg
    22021 gaacatattt taaaaattct tgaaaatcca taatcaaaac ctatttttgt acattggaaa
    22081 ctgttataaa tataatgcaa aaatttctaa aaacaaattt taatggctat tccaaaggat
    22141 ataacaataa tcctaaatct taaactctaa aacaccaaac tgtaatgtat agcaaagagg
    22201 aagcgcaacg attaaaacga gaattctgga tagaatttgc cgaaaaatat ccccgaaaat
    22261 ggattttgta tgacaccaaa atcaaggatt tctcgtttaa attttatgtc gataacaaaa
    22321 aagcacaagt cctaatagat attgaacatc gcaatgacga aaaacgaatt gtttattttg
    22381 aaaaattaga atctttaaaa acagttttgg aggatgattt tgtcaaagat ttggtttttg
    22441 agaaaaactt tactctcgaa agtggcaaaa ctataagcag aatttgggtc gaaaaaacag
    22501 gcataagcgt tagtaataga aagtattggg acgaaatatt cgactttttc tacgaaaaaa
    22561 tgaatgcatt agaaatgttt tattttgaat atggagacta cattcgagat ttggaattaa
    22621 atacttaaaa attacttaaa ttcttacagt actttcaaca aaaaatatat atttgagaaa
    22681 attataatac atatgaaaaa aatatattgg gtcttttttg tggtactttt tacctttact
    22741 tcattagccc aaaattcgtt ggaagattac aaaaaaaaat atcccgattt taatgaatta
    22801 attctaaaag atcagcaagc ctatgatttt tcaatagaaa acgacaaatt aaaagtcatt
    22861 gaaaacagtc aatatgaatc tttaataatg actgacaacg gcataatcaa caacaaagaa
    22921 tcttttagtt attcggaatt agtcaaactt atgggctatg atgcctattc ggtggttaca
    22981 gaaaatggca aggaaaagaa aattaaagtt acccaaacca acgaagtgca atcaggacaa
    23041 agttcggtgt tttatgatga tgtgaaagtg cgtcaactta tttttccaaa tctagaagct
    23101 ggagccaaaa aagtatatca ttatcaaact gaaattatag atccattctt gcttcataaa
    23161 tatgttttcg gaagcaattt gcccataaaa aacgctgttt tagaaatcaa aacagacaaa
    23221 aacatcaata ttggctacaa aatatttaat gatccaacaa attcaatcga attttcaaaa
    23281 accgaaaaaa aaggaaaatg gatttatcaa tggactctaa aagaggttaa accagtaaag
    23341 tttgaatcga attcgccagg ttttttacac ctagttcctc acataaatgt gtatattaaa
    23401 gactatacca taaacaacaa aaaagtggaa gttttaggag atatagacaa gttatatacc
    23461 tattacaaag gttttacaaa gaacattaac caaaaagaag atgcaaactt aaaagcgatt
    23521 actctcaaat tgatagaaaa tctaaccact gatgaagaga aaattaaaag tattttttat
    23581 tgggtgaaag acaacatcaa atacattgct ttcgagaatg gttatgaagg ttttattcca
    23641 agagaagcca gcctaatttg tgaacgaaaa tttggcgatt gcaaggatat ggcaagcata
    23701 attaccacaa tggcaaaata tgccaatgta aaagacgtat ttctttcgtg gattggaaca
    23761 cgtgaaattc cttattctta taacgaaatg ccaactccag cagtagataa tcatatgatt
    23821 gctgtgtata aaaaaggcga caattatatc tttctggatg ctactgacaa agaaacgctg
    23881 tatggaattc caacctcttt cattcaagga aaagaagctt taattggcga aggcgaaaac
    23941 tataaaatcg taactgttcc tatcgtagct gccgaaacca atgaagtaaa cgaacaagta
    24001 aaattaagta tcgaaaaaga caaactcgta ggttctggaa aagtacaatt caaagggtat
    24061 agccgcagta atattaccct tcgaatggga gatgctacga acaaaactag attcgaaatg
    24121 ataaaaagtt tggttcttaa aggcaataac aaatttaacc tcaaagaata ttccgaagaa
    24181 aatcttactg acagagacaa accttatcaa ataaactaca atttcgattt gcaaaattat
    24241 atagtcaaag ccgataagga aatttatatc aatctttttt tggaaaaacc atttgaaaaa
    24301 ttaaccatcg aaaaagaccg atttgcacca tttgaattcg agcatttaac atttgtaaac
    24361 agccaatacg aattagaaat ccctaaaaat tgcagcttaa aataccttcc taaaaatttt
    24421 agtttggaca atgattatgt aaaagcaaat ttcacctatc aaaatcaaaa caataaaata
    24481 acattgtcaa tcaagctgca acaaaagaaa ttattgattg caaaatctga ttttgagctt
    24541 tggaataaga ccataaaaga actaaaaaac aattactctg aaaccctaat tttattagaa
    24601 aaataatgaa aatcaatcca ttaaaaatca tttttacact agttgtatta ttcaactcat
    24661 cggcattttt agcccaagaa tattcgttta aatcctatga ttggaatgaa aaagaaacta
    24721 cagttagcgt tcctgaaaaa tacaaaaacg aaaacgaagt catcctaaat cgaactgaaa
    24781 aaattgaatt tgtgattgtc ggaaagacag ccaaacaata tcacttggtt cacgaaaaaa
    24841 tcttcatcaa ctcagacgat gccatcgaac gcaataataa aatttacatt ccctttagtc
    24901 tagacgaaag tgtactggcc aacaaagcac gagtaattct taaaaacggc acaactattc
    24961 ttttggataa aaaagatatc aaagaagata ttgaccaaga aaaaggaatg aaatacacct
    25021 attttgcagt aaatggactt gaaaaaggcg ccattatcga aaaattgtat gtccttgaag
    25081 aaagtccaga atttactgga caaactatca aaatgcaaga cgaataccca attgctgatt
    25141 tgaattttga attaatttat ccgaattact tagtgtttaa aaccaaatcc tataacaaat
    25201 taagtgaacc tacaattgat gaaaaaaaga ttcaaaacac caacgtttta tcaattatag
    25261 aaaaagacat tccagcttta gacaataatg aacaatattc aaattgggct ttgcagttaa
    25321 aattattccg atacaaactc gatgaaaatc tattaagtgg cggtagaaat ttaaataatt
    25381 tcaaggagtt ttctaataat ttatacacaa gacttaaccc agaattagac aaaaaacaac
    25441 aaaaagcaat cgacgatttt tgtgctacaa ttccaaaatc gaatgatgaa caagagcaaa
    25501 tttggaacat cgaaaacaaa atcaaaaaaa ccattggcta tgatagatat atcgattcta
    25561 aagaaaatct atcggatgta attgtaacca aacaagccaa tcaatctgac atactaaaat
    25621 tttattcggc tgttttcaaa tacttttcta ttgaaaataa tatggttttc acttcaaata
    25681 ggtataaaat tccatttgat aaagactttg aaagttttga aaatctaaac gaacttttgt
    25741 tctattttcc aaccatcaaa aaatatttaa caccaacaga aattgaatat cgaattccgc
    25801 tttttcccaa tactatcgcc aataacaacg gtttattcat caaagaaaaa acgtttggag
    25861 gagtaaaaat gggaattggt gaaattaatt ttatagaaat tccaggaaat gacatctctc
    25921 acgatataat gaatatcacc atcgatttta ctcaagatat tgaaaatcca ttaattacaa
    25981 gcaatctaac ttttggtggt tattcaggtt tgaattttca acccataaaa gattttgctt
    26041 cggctgatca atataaagcg gttttgaaaa gcattgccga aaattatacg gtagaaacag
    26101 aatacaaaac tcttaccaca gaaaatgatg gaatagaatt tattggcaaa aaatcgtttg
    26161 tgttaaatgt aacgttcgac ggaaaagaat tgaccaaaaa agcgggtgaa aattacttgt
    26221 tttcagttgg tgaaaccatc ggaaaacaaa tggaattcta ccaagaaaac aaaagaacat
    26281 tgcccgtaga aattgactat ccacatcttt atacccgaaa tatcaaaatc atattgccaa
    26341 aaggggcttc agtaaaaaat ctagataaat ttgtaatgga ttataaaacc caagtaaatg
    26401 gcaaaactga agctggtttc aatagtaaat acactaaaac tggtgatgaa attttaatcg
    26461 aaaacactga attttacaac attgtcaatt atcctttaaa ccaatttgac gattacaaag
    26521 cagtaatcaa tgcagcagcc gattttaata aaattgtgat tatcgttaca aaataaacaa
    26581 gtagtctaaa aaaatcagac cgcaaattcg catattattt tcagattcaa tctggaactt
    26641 gcgaatttgc ggtctttttt tgggatgtat tttttaagat aatgtactaa aaaatgattg
    26701 gtgatttttc attttaaatt aatcattcat cgaaatcaaa aactcttcgt tatttctagt
    26761 tttcttgaaa cgatcgttga taaaatccat tgcttctact gggttcatgt ctgaaagata
    26821 ttttctcata atccacattc tttgaagcgt ttttgcatct aacaataaat catcacgtct
    26881 ggtgcttgaa gaggttaaat caatggctgg gaaaatacgt ttgttggcaa ttttacgatc
    26941 caattgcaat tccatattac ccgttccttt gaattcttca aaaataactt catccatttt
    27001 ggaacctgtt tcggtcaaag ctgtggcaat gatactcaat gaaccgccat tttcgacatt
    27061 tctagcggca ccaaaaaatc gttttggttt ttgcaacgca ttggcatcta caccaccact
    27121 caacacctta ccagacgcag gttgaactgt attgtaagct cttgccaaac gggtaatcga
    27181 atccaataaa atcactacat catgaccaca ttcaaccaat cgttttgatt tttcgagaac
    27241 gatattagca attttcacgt gttcttgtgg ttctctgtca aaagtcgaag caattacttc
    27301 accgcgaaca cttctaatca tatcagtcac ctcttctgga cgttcgtcaa tcaataaaac
    27361 aatcaaatac acttcaggat gattggctgc gatggcgttg gcaatatctt ttagcaacat
    27421 cgttttacca gttttaggct gtgctacaat cataccacgt tgaccttttc caattggaga
    27481 aaataaatcg ataattcggg tagaaatggt actttgtctt tcagctaatt tgaatttttc
    27541 agaaggaaaa acaggagtta aatgttcgaa tgatactcta tcacgaacta cttgtgggtc
    27601 gtgtccatta atttttaaaa cacgaactaa tggaaagaat ttttcacctt cttttggagg
    27661 acgcacaact ccttttacgg tatctccggt tttaagtcca aataatctaa tttgggatgt
    27721 cgataaataa atatcatctg gtgaagccaa ataattataa tctgaagatc gaagaaatcc
    27781 atacccatca ggcatcattt ctaatactcc ttcgctttct ataattccat caaattcaaa
    27841 atcagaatct ctaaagttgt ttttcttgtt tttgaaattt ggatttgcat ttccattttg
    27901 atttggattt gcatttccat tttgatttgg atttgcattt tgatttggat tttggttgtt
    27961 tttttgctta ttgagttggt taggatttat tttttttgaa ggaatttcaa tctcattatt
    28021 ttcggcagtt tctgcttcta aatttgcttg actaacttcg tttgattcgc ttcctacact
    28081 agcaaatttt gcttcttctt tttgcttttg caaagcaact ttcttttcgt aagctgattt
    28141 attaaattta atcactttac ttgctttctt ttcaattgga tttacttcaa ccgaaatttc
    28201 atttttaacg ggttcaactt ttggttcaat cgctatttcc tctttagcaa aaagtgaatt
    28261 tgtagcattc ttttggatgg caactttttt gggagctata attcgagtac gtttaggttt
    28321 ttcaacttct tctttaggtt cagctggctt acttcctgaa tttgaatctg tagttttagc
    28381 ttgaagttct aaaatttgat tgattaatac ctcttttttt acaccattaa attttattgt
    28441 tttagccgct ttagctattt cttgaagctc agaaagcttc atttctttta atgcagaaat
    28501 gtcaaacata aatgttctat gagtttaaat aattggggaa tactataaaa gtgtgtagta
    28561 aaatttgatt ttgaattgac cgaagaatga agtacttacg gatttgttat gcaatattac
    28621 gaataaattt taatctacaa tagtatttat taaaaagaaa atatattttt gtcaaacaat
    28681 ttaagacaat gttacaaaga attcagacca tttatttact tctagccttt gttgcaactg
    28741 gagttttacc cttcttcttg ccgctttgga caatgagtga tggcaaagac tacctattta
    28801 tgcaaaatca agtatatgtt gtattacttg gattgagcac aacacttacg attgtgagta
    28861 ttgtttccta taaaaaaaga caaaatcaat ttgttttagg cagattgaat ataattttaa
    28921 atttaatttt attaggcttg tttgtatatc gttcactaaa tgtatctgga gaaacactag
    28981 ctgtttctga gaaaggtatt gggatgtttc tacctattgt tgctatcgtg ttgttggttt
    29041 tagccaacaa ggccatcaaa aaggatgaag atcttgtaaa atctgtggac cgtctgagat
    29101 aatcctataa acttagttta ttagtgcgaa ggaaacccga atgtaatagt tcgggttttt
    29161 ttgtggttag attttttttc aaccgctgat tggcagaata ttcaatattt ataatttgca
    29221 aatttgcggt aatcttactt cttccctatt tcaacaatct ccaaatcctt aattttatcg
    29281 ccatcaataa caaaccgaag cattgttctt acttggtgaa aaccactttt tcctacagct
    29341 ccaggattca tatgcaagag attgtttttc ttgtcaaaca tcactttcaa gatatgagaa
    29401 tgcccgcaaa taaataattt tggaggattc gtcattattt cagctttaat actagcattg
    29461 tattttccgg ggtaaccgcc gatatgtgta atccaaacat ctacaccttc gcacataaat
    29521 cgattgtgca acgaaaattc cattcgggct ttgtcgtcat caatattacc ataaactcct
    29581 cgcaaaggtt tgattttttt tattgtatcg gtcaccacta aatcgccaat atcaccagcg
    29641 tgccaaactt catcggcttg ttctacatat ttcaaaatgg tatcgtcgat atgactgtgt
    29701 gtgtctgaaa gcaggagaat ttttttcata tttgttttta acagtaaaat cgcaatgatg
    29761 caaagttata atttcatccc gaagcttcgg gagaaaaatt aactgttttt aatttacttt
    29821 gtcgttgtgt tagttttggg aaaattttaa aagtataatc aacttaaatc tttgtaaatt
    29881 tgccgatatt aactcattca ttgatcactt tttcggcatt attgactaaa tactgaacac
    29941 tgaatactgc atactaactt tgaggtattt tataaaacta gcttacaacg gaacccatta
    30001 tcacggatgg caatgtcaac ccaatgcatc atcggtacaa gaaactttga acaaagccat
    30061 ttcggtcatt ttgaacaacg aaataagcct tatgggagca ggccgcacag acactggtgt
    30121 tcacgccaaa gaaatgtatg ctcattttga ttttgaaaac ccaattgatt cctcgagttt
    30181 agttcacaaa ctcaattcct atttacctaa agacattgta atttatgatg ttattctggt
    30241 tcacgatgaa gctcattctc gttttgatgc tataaaacga acatatgaat accatatcaa
    30301 tacttttaaa gatgcttttt tgcaagatgg aagttggtat ttccaccaaa aattggattt
    30361 ggacataatg aaccaagctt cgaaattatt attcaaccat attgattttc aatgtttttc
    30421 taaagtaaat accgatgtaa atacttttga ctgtactatt tttgaagcct attggaagca
    30481 agaaaataac caaattatat tcaccatttc ggccaatcgt tttttgagaa atatggttcg
    30541 ctctattgta ggaactttaa tttatgtagg tttgcacaaa attacattag aagattttaa
    30601 ctcaattata gaaagtaaaa gccgcgagaa ggcaggattt tctgtcccag cacacggttt
    30661 atatttgact caaattactt atgattacct aaaatagccc cgatagcagt gaaaatcctt
    30721 ttttgttttt gctgccaaaa acaaaaaaga ttgcaacgaa tagcgggaaa cagctcctaa
    30781 aaaatatatg aaagcaaaag cattcgatac acgattattc aaaagaatat taaaatatac
    30841 caaaccttat caattccgat ttaatggagt gattttcttt gctattttct tatccgtttt
    30901 tgcagcagta cgccctttca ttttgcaggg aactgttgat acttacatca aaccaaaaga
    30961 cacttatggt ttattgatgt atgtaacttt gatgggagtt acattagtat tagaagttgt
    31021 atcccaattt ttctttgttt attgggctaa ctggcttgga caagatattg tgaaagacat
    31081 tcgaaataaa atgttcaaac acattctgag tttcaaaatg aaatattttg acaatgttcc
    31141 ggttggacaa cttgtgacac gttctgtttc agatatcgaa gctattgctc gcattttcag
    31201 tcaaggatta tttatgattg ctagtgattt gatgaaaatg gcggtttgtt tattgatcat
    31261 gttttggatg aattggcaac tttcgtttat tgtagttata gccatgccaa tattagtttt
    31321 cttcacccga atttttcaaa ggaaaatgca agttgctttt gaagaagtga gaacacaaat
    31381 tgccaacctt aatacttttg tacaagaaag agttaccgga atgaaaatcg ttcaactctt
    31441 taatcgagaa gatattgaat tcgaaaaatt caaggctatc aatcaaaaac acaacaaagc
    31501 ttggattaaa accatattat acaactctat tttctttcca attgccgata ttatttcatc
    31561 cttaacactt gcttttattg ttttatatgg tggaattcaa atactaaatg gaagcacatt
    31621 tactacccta ggtcaaatga cggcttacac gatgtttatt gcaatgttat tcaatccttt
    31681 acgtcaaatt gcggataaat tcaacgaaat gcaattggga atgattgctg ccaatcgtgt
    31741 ttttgatatt ctcgatactc aagaccaaat ccaagataca ggaactgttg aagctcctgt
    31801 ttttaaagga aatattcaat tcaaagaggt tcattttggt tacatcgcta atgaagaagt
    31861 aataaaaggc attgatttag atgtaaaagc aggacaaact atagccattg ttggagcaac
    31921 tggagctgga aaatcgacaa ttattaactt attaaatcgt ttttatgaaa tcaatagcgg
    31981 aacgatttgc attgacgatc acaacatcga aaactatact ttaggttcat tgcgcaagca
    32041 aattgcagta gttttacagg atgtttttct ttttgccgat accattttca acaacataac
    32101 gcttaataat cctgatattt ctcgggaaca agttttggca gcagccaaaa aaattggcgt
    32161 tcacaaattc attatgagtt tgcccgataa ttatgatttt gatgtaaaag aacgcggagt
    32221 aatgctatct tctggtcaac gccaactgat tgcttttctt cgtgcctatg tgagtaatcc
    32281 gagtatttta atattagacg aagccacttc ttcaatcgat acctattcgg aagaattgat
    32341 tcaaaaagct actgaaacca ttacaaaagg aaggacttct atcgttatcg cccaccgatt
    32401 ggccactatt cttaaagccg ataaaattgt agtgatggac aaagggttaa ttgtagaaca
    32461 aggaacccat caagaattag tagaaaaaat aggtggttat tacaaaaatt tatatgattc
    32521 gcagtttgta acagaagaca attaaattta tatacttaaa tcttccaagt aactttacaa
    32581 attcatgaaa aaattcatca gttacctaat cattataata acttattctt gtattccatt
    32641 gaaaattgcc ccaagcatag aaggtgaaaa aataacaaac gcaaaaaaat ttaaaaaaga
    32701 ccttccaaat ttttacggat ttatttttga agacaccaaa aaggccaatg agttttacaa
    32761 ttttatcaat actaaatata atttaaagca tattaatgtt gaatcaaatg ttccaattat
    32821 caataataac aatacctatt atttatcttt ttttgagcga gaaagaacca caaagacttt
    32881 aaatttggtt ccaatagcaa ttgatgcaga tagaaacagc aaaggaaaag aaccaatact
    32941 agaagatctt tacacctcta gaactggttc ttggtatcta gttctcactt taacagaccc
    33001 cgaaataaaa gattgcctta atccaaatta tccgcaacgt gaagagatca taaaacattt
    33061 gaaaatggta agggatgaa
//
LOCUS       DAOSNN010000003        29672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_1571_length_29672_cov_5.778202, whole genome shotgun sequence.
ACCESSION   DAOSNN010000003 DAOSNN010000000
VERSION     DAOSNN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 29672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29672
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1571_length_29672_cov_5.778202"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /locus_tag="PLQ70_00290"
     CDS             complement(<1..103)
                     /locus_tag="PLQ70_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQE33083.1"
                     /translation="MSKVEDFLTKEEEQAIVEAIRMAEKETSGEIRVH"
     gene            complement(150..737)
                     /locus_tag="PLQ70_00295"
     CDS             complement(150..737)
                     /locus_tag="PLQ70_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQE33084.1"
                     /translation="MDFKKFLPWIVGIGFILIIAFWVMGIMNTGLQKDQAVNKEWGNV
                     NTAYQRRNDLIGNLVNTVKGAADFEKSTLTAVIEARAKATSVTIDPANVTPEQLAAFN
                     SAQSGVSSSLSRLLVSVEQYPTLKANENFLKLQDELASTENQILTARTRFNESVQDYN
                     GYVLAMPQSIFLGKYKEKPYFKAVEGADKPVEVKF"
     gene            complement(797..1129)
                     /locus_tag="PLQ70_00300"
     CDS             complement(797..1129)
                     /locus_tag="PLQ70_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQE33085.1"
                     /translation="MHIELSADKRYYSIGEVAKAFDVNASLIRFWDSEFDILKPKKNA
                     KGNRMFTPEDVKNLQLIYHLVKERGFTLEGARTHLKEGQKKTLDKFDIISKLEAIKVQ
                     LTNIKNEL"
     gene            complement(1182..2159)
                     /locus_tag="PLQ70_00305"
     CDS             complement(1182..2159)
                     /locus_tag="PLQ70_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQE33086.1"
                     /translation="MAKVKYYYDSENLAYRKIITKTRKKIGVVLLFLVASGLFGVLSF
                     IILLNTPYFETPKNKKQAREIENLKLRYAILNKKMEEVEDVIEIIEERDNNLYRVYFN
                     ASPISEEERKSGFKEVNRYKDLEGYDNSQLVTNTIKRIDVLRKELAIQSKSLDDILKM
                     AKAKDKLLAAIPAIQPVKNENLKSMVSGFGYRTDPFTKARKMHEGMDFTAKTGTPIYA
                     TGDGVVAKADNTASGYGNHIVIRHGFGYETLYGHLSKYKCRAGQSIKRGDVIGYVGST
                     GRSEGPHLHYEVHKNGKVVNPLNFYYGNISAVEYVAIAQAANQENQSFD"
     gene            2334..4964
                     /gene="alaS"
                     /locus_tag="PLQ70_00310"
     CDS             2334..4964
                     /gene="alaS"
                     /locus_tag="PLQ70_00310"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQE33087.1"
                     /translation="MKSQDVRKQFLDFFESKGHLIVPSAPIVLKNDPTLMFNNSGMAQ
                     FKEYFLGNATPKSKRIADTQKCLRVSGKHNDLEEVGIDTYHHTMFEMLGNWSFGDYFK
                     KEAINWAWELLTEVYKIPKDILYVSVFEGNAEENVPFDQEAYDIWKTLIDEDRIILGN
                     KKDNFWEMGDQGPCGPCSEIHVDIRSAEEKALVSGKSLVNNDHPHVVEIWNNVFMEFN
                     RKADGSLEKLPAQHVDTGMGFERLCMVLQGVQSNYDTDVFTPLIREIELITGAKYTIK
                     ASNEEEEKINIAIRVIADHVRAVAFSIADGQLPSNTGAGYVIRRILRRAIRYGFTFLN
                     TKEPFIYKLVETLSAQMGDSFPEIKSQKTLCSNVIREEENSFLRTLDQGLVLLENIIT
                     NSKDTVISGKKAFELFDTYGFPIDLTALILSEKGLSLDEAAFESELQKQKERSRAASK
                     VKAGDWQDLIDDEEQEFIGYDYTEAKVKITRYRKVESVKDGEIFQLVFNMTPFYPEGG
                     GQVGDAGVLETANGDVIYILDTKKENNQIIHFTKELPANLTEIFKVIVAPDRRRKSAA
                     NHSATHLMHQALRSILGTHVEQKGSLVNSNNLRFDFSHFSKMTDDELKQVEDFVNARI
                     QEQLPLIERRNIPFQQAVDEGAMALFGEKYGDAVRAIKFGESMELCGGIHVGNTADIW
                     YFKIVSEGAVAAGIRRIEAITSDAVQTYFETQEKILDEVKTILKNPQDVIKSVHSLQD
                     ENAKLKKQLEVLLKDKAKNMKGELAQELQEINGVQFLAKQVDLSPEGAKDLAYELGTL
                     GKNIFLVLATAEEGKPMLSCYISKELVAEKNLNAGQVVRELGKFIQGGGGGQPFFATA
                     GGKNVDGIPEALAKAVDFVK"
     gene            complement(5032..5433)
                     /locus_tag="PLQ70_00315"
     CDS             complement(5032..5433)
                     /locus_tag="PLQ70_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33088.1"
                     /translation="MKHLLTTLFAVCLSYTAAVAQEKEAEKAPQEKTVSKEEKEAAKA
                     KKEANLLEVFKIIQATPEEQAKMREFIKESGEYGKELRADPDFTEEEKIMKLKVYAKI
                     KNARLRELLGDDRYKLYRDAVNAQKGRALKK"
     gene            5774..6565
                     /locus_tag="PLQ70_00320"
     CDS             5774..6565
                     /locus_tag="PLQ70_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33089.1"
                     /translation="MNKAKKIVGVLVVVLMTLCSCTTETVEQTSFLKKIVEVSVDGTS
                     NTTNLIYDGNKILTIDKVDQLLKFYYTGNFITKVVELDKTTQHTNTLDYSYSVEGQLT
                     KIISSDNYLINYVHNSDGSVSYEKLTKDSTKPEIKNYHGILYFQNGNLVKDDKTQDDA
                     GSGILIKNTINIQYDYKNNALKNILGFTKLLDYSRLVSTNNEVVSTEAYSVKDVDNDQ
                     ITSSIKRIDSQCQYNSNGYPTEIVSEKILFGGSDSNHLKSQLFYN"
     gene            6590..7540
                     /locus_tag="PLQ70_00325"
     CDS             6590..7540
                     /locus_tag="PLQ70_00325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HQE33090.1"
                     /translation="MQFRTKISIPQSDFPIDYDSKILSLGSCFAVNMAEKFDYFKFEN
                     TCNPVGILFHPLAIEKLIDFAVSGKQFCAEDVFFHNERWHCFDVHSDCSHSNKDELLL
                     NLNTILETTRLQITESTHVIITYGTSWVYRNIATKEIVANCHKVPQKQFGKELLSVGA
                     IEKSIQNTLDLIQKVNPKATIIFTVSPVRHLKDGFVENQQSKAHLITAIHTAINQQLS
                     TNNYFPSYEIMMDELRDYRFYAEDMIHPSQVAIDYIWQRFSETSISEESLSVMKEVET
                     IQKGLQHRPFNPNSESHQQFLSKLQDKMMQIQKQFPQIQF"
     gene            7621..7995
                     /locus_tag="PLQ70_00330"
     CDS             7621..7995
                     /locus_tag="PLQ70_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33091.1"
                     /translation="MKKNQLILFLGLFLLLFSEPVFAQKKVLTDEEEVTEMVTKEMDE
                     LFKDKDFLKQKDKKFKDVKGTIVSDISVIQSGKVSTLFKVDSDINNPSFTNFLSDYII
                     NYKFKFKLQKQQRYKIRYTVTF"
     gene            8020..9846
                     /locus_tag="PLQ70_00335"
     CDS             8020..9846
                     /locus_tag="PLQ70_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQE33092.1"
                     /translation="MKKLTLLMLSICMMATVPTSAQFGKLLDKAKNAVSGSGAGKKTG
                     SFATVWESEFGNKATRLAVCGGTGDFIIGTDDNSASVLDSSGKAIWNGDYKKITTNGT
                     NKSEYQYTQWKSDKGGYLFLFDERKLGTDRVACLDIETGKELWSSESYQNLIPKGTKS
                     EDGADQGELDTVKYISELDAFLISQKASIILVKANTGEKIWETNRFKGGVGKYIYNKE
                     KNEIIMANFKPTALGALFAGFKNQLVRVNASNGEILWDATFVGTVEKELVTRRAILDL
                     WIRDNKIFLYLNGLMVYDYNNGQKLWEAIYENDMSGGGGGMFSNTQSKKYYRTIADPL
                     FTENAVYIVMLGTRDRTKYVEKHDLDSGKLLWASEKVTGAFCVPNIYKTGDKVLIQIG
                     GKVQVQEYRMETQTSPFGGTYKASVPYIYWDYKNQKNGVLCMDDTSGKLAWRSDKFDK
                     RITDLIINEEKTVFVGDGDEFYSYEIASGKQLFDVKHNDAGVGKATDVIDFEDKVVVL
                     SEKGLASYNKKDGSRAYATEKIKGVDYFYHIGDNYFLRDQRNSKNIIYGIDMTNGETK
                     GSVQSKGKGGSPTYGDGIDITSDGEYIFAFKGRKVEKIKVNN"
     gene            9968..11392
                     /locus_tag="PLQ70_00340"
     CDS             9968..11392
                     /locus_tag="PLQ70_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33093.1"
                     /translation="MKKTIFLCSLFFVVFSIKAQQIQWASKLIKFSTDLGGKQNGIKR
                     ILGKPDAFPQGGSSANAWMPKNALDGREIIEVGFEKPQTVKQVAVCENLNSGCVIKIS
                     VDNGSGNYETVWTRKRDWKTPTFKSTASADHAYYFGRKRRKVQEVPNVFNPGIEYAVL
                     ENAVANVVAIKVEFNFALLPGPKQIDAIGISDSEKPIEIKINSNSAFENLPNPESLEF
                     DNSEVSNVIVSQDGQKIFYSAFAENKDVVFSSQKQADGKWSKPVVEPSLSLNNDYNYL
                     EFYSDTFILKGGVGYSKNTTETGFEFIENKNGSYQSLKPLKIAAYNNYEKTADITITN
                     DGKILIMGIETDFTQGGTDLYFANRKEDGTYGLLQNMGKIINSAADEGMPQLLSDTKT
                     LFFSSIGFSSYGNYDLYVSYRLDDSWKKWSEPINLGNKINSDSFEGSPFYDETTEMLY
                     FTRVVEGKSSISRVKIPKKELVKN"
     gene            11510..13030
                     /locus_tag="PLQ70_00345"
     CDS             11510..13030
                     /locus_tag="PLQ70_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33094.1"
                     /translation="MCILSYSASFSQISLVKEIYAGDSYYPPKELLELNGRLLFTTDA
                     PAGSVSSFWKSDGTLAGTTILGTFGPDQRVSFSKKFNNFIYFNVGKNGAVQSLWVTDG
                     NGVYLVKSLGSTSATGELTVFKNALYFNTSSLFAQGIHKSDGTAAGTVGFANITATSP
                     LVVSGTNMYFTTYVASVGNELWKTDGTVAGTVMVKDIYPGSVDAQISQMTDINGTLFF
                     IANNGVNGNELWKSDGTAAGTVMVKDITAGGIYSHSILNYLTNVNGILYFTKGNYELW
                     KSDGTDAGTVLVRPSTSISPTNLINVNGTLFFECGTLSTGAEICKTDGTTSGTVMVKD
                     INVSGTGYGGFINPYVVGQTVYFRAQNGSNGYELWKSDGTEAGTQMVYDTNVGSANFN
                     PEKLTKAGNKLFMVGQTAAEGMELFVYNIPSLSSNTIDGITPLLVYPNPSKGNFNIEI
                     DENLIGAKASVFSLLGQKIKDFELKSTITNQFLNKGIYLLEIEKAGNKTSKKLIVE"
     gene            13133..14518
                     /locus_tag="PLQ70_00350"
     CDS             13133..14518
                     /locus_tag="PLQ70_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33095.1"
                     /translation="MEKQLLFFVFFLCIGKSSFAQYTKLLDFDGTAKGKYPYGTLYSD
                     GIFLYGTTSIGGTNDLGTVFKIKPDGTGFSKLLDFAGTTNGSNPYGNLISDGTFLYGM
                     TVNGGANNLGTIFKIMPDGTGYAKLLDFAGTSNGGNPWGSLISDGTYLYGMTFVGGSN
                     SMGTVFKIKFDGTGFTKLLDFAGLSNGRNPRGDLFYDGTYLYGMTSVGGSDNFGVLFK
                     MLPNGTGYTKLLNFDSVTNGRNPRGNLIFDGTYLYGTTSGSGTNSSTNFGTIFKIMPN
                     GTGYVKLLEFDGVTKGRYPYGSLLSDGTYLYGMSYNGGIDNFGTLYKIMLNGTGFTKL
                     IDFNITNGQIPWGSLIAVGSSLYGVTAGGGTALSGVLFKYGISTLSVAKNQKPNDFKI
                     YPNPSNGNFNIEIDENLIGSKATIFNLLGQKIKDFDLKTTTTTEFLNKGIYLLEIEKD
                     GSKTTQKLIVN"
     gene            14621..15805
                     /locus_tag="PLQ70_00355"
     CDS             14621..15805
                     /locus_tag="PLQ70_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33096.1"
                     /translation="MKRIITILLIVFFTTITNAQWTLCNNPSGALPFSLTIQNDTLYL
                     GTLSNGIYRSTNGGSSWTQINNSITSMQIWNIAYVNGSLFASSTNGTVFKSTNGGDSW
                     ILSNTGISTTTIIRNFAFFNNKIFATSTNKGVYISSDNGSSWTQHNTGLVGLVAEPLL
                     VVDTDLFVGVNQKVYKYDSVNQNWISKSVGIPNNTVTSLTYTKDNLQNISLFESTVNT
                     NDVTISTNKGDSWSVVKNGLPSVGVYSLLGIGTEVYLGNDYGVYKTTNQGTNWVDISG
                     FTNASPAKFLSKSSTDLYVIQEAKIWKKSLNNLGVSGLENGFESQTIKIYPNPTSGNF
                     NIEIDENLIGAKATIYNLLGQKIKTLDLKTTTTTEVLNKGIYLLEIEKDGNKTTQKLI
                     VN"
     gene            15943..16125
                     /locus_tag="PLQ70_00360"
     CDS             15943..16125
                     /locus_tag="PLQ70_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33097.1"
                     /translation="MKVLGEIIVQISRDKSGKGNQIRLSDELNSKLKAFNSALGSDIF
                     ELNKELFEILSKNNLA"
     gene            16368..18230
                     /locus_tag="PLQ70_00365"
     CDS             16368..18230
                     /locus_tag="PLQ70_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQE33098.1"
                     /translation="MKIARLFLFLLLCLVQFSYSQEAKKLIYKAFGSKDSSDYYFKLA
                     KKNIKTPADEAEYYFGKNGRCNDYNQPDSCVFYGLIAIQKFKKIGNSNSLYYVYNNIA
                     ASYRKQGQYEKAISYILDGLKLAETENKEYWLANYNSNISLNYHDFESYQKGILYGKK
                     ALNYGLKAKNRDKKSITFALNAIAINFDDWNKPDSALYYHKKVFNYVKGKDTLDYSHT
                     YNNIGNTYMKQKKYKEAESWIKRAMIIGATNSNGVKDASYYYENATHYTNLATIAYNL
                     DDFAKAETLFDKAYFFVKNSKSAEKFRDYFQQRYLFNKKRKNLEKTVEFQENYIKLRD
                     SVFELERAKTFTELETKYQTAKKEKLLIEKEAEAKQKNTMLIGISLLAFFIALIGFLI
                     YRQQKQKNVQQKQEYKLKKEISKIETHNKLQEQRLSISRDLHDNIGAQLTFIISSVDN
                     IKYAFDITNEKLDHKLSHISNFAKETILELRDTIWAMNSNEITFEDLEIRINNYIEKA
                     KEAKDQISFSFAIDPELKTQKLTSVQGMNIYRTIQEAVNNSIKYANASIISINAKKIE
                     NRIKISIQDNGIGFDLVTVEKGNGLKNMQKRIEEIGGEFHLSSSNEGTLIEVIL"
     gene            18234..20156
                     /locus_tag="PLQ70_00370"
     CDS             18234..20156
                     /locus_tag="PLQ70_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQE33099.1"
                     /translation="MKKKLLLLLLITTTAFSQNAQEIIDGLKKDLKNSPDAKKTATIY
                     SDLTWYYSTVSIDSALHYGNKAVIASTKLGDSTLIAQVFSDVGAVYFRKGDFQNSKEN
                     YLKAYRIRKARNDYRGLAKINNNLANIYEKNGQYKQAMTSFLDALQFFESIKDEKNSH
                     ITKGNIGLILLKLKNYPKAFTYINDVVEYQEENNATAELCVSCLNLGNVYLQMNDTLN
                     ALKQYNKSLKYCNNVGNKKGVSSAYNNIASIKTEQKKSKEALVLYAKSQQVRKQLNSD
                     IDKANFDLNVAEEFMHHNKLNEAKQLLLNTKHVFEKEKLYDKLQINYKSLIRVYAKLN
                     QLDSVSIYVDKLLVINNQLLENSVVKQTAELETKYQTEKKEKLLLEKEVEAKQKNTLI
                     LGISVLAFFIALIGYLIYRQQKQKNIQQAQEYKLKKAIIKIENQNKLQEQRLNISRDL
                     HDNIGAQLTFIISSVDNIKYAFDITNEKLDNKLSNISSFAKETILELRDTIWAMNSNE
                     ITFEDLEIRINNYIEKAKEAKDQISFSFAIDPELKTQKLTSVQGMNIYRTIQEAVNNS
                     IKYANASIIAINAKKIENRIKISVQDNGIGFDLATVEKGNGLQNMQKRIEEIGGEFGL
                     DSSSEGTSITVLLKKN"
     gene            20156..20806
                     /locus_tag="PLQ70_00375"
     CDS             20156..20806
                     /locus_tag="PLQ70_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQE33100.1"
                     /translation="MMIKIAIVDDNSFLIKTVQEKLSFFEDFSLKFTAVNGLDLIEKL
                     EKNHNVDLILMDIEMPKMNGIEATCLVKQKYPQIKIIMLTVFDNDENIFNAIKAGADG
                     YLLKEVNPKDLNQGIIETMNGGATMTPSIAMKTLKLFRNPIAFETISDKEEYNLTSRE
                     IEVLEQLSKGLKYNAIAENLFLSAGTIRKHVENIYAKLQVHNKLEAIQKAKNNKLI"
     gene            20874..21122
                     /locus_tag="PLQ70_00380"
     CDS             20874..21122
                     /locus_tag="PLQ70_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33101.1"
                     /translation="MEILVYLGLILGLLYLIFKSRKIRKKYKIKSKKIKAEIKALKLK
                     RALIEERSDANHLITIDFYKMLEQVSDLHKLIITKYLK"
     gene            21168..23024
                     /locus_tag="PLQ70_00385"
     CDS             21168..23024
                     /locus_tag="PLQ70_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE33102.1"
                     /translation="MNKKILLILFVFSAFLSGAQSNKEFLEKAIVSKDSSDFYFQKAK
                     NQIKSKDDEAAFFYYKNALCTELEIQDSAIYYGKIALKKFENSKNYNSILNLYYRIAN
                     VYNNKGQYEKSIDYNISGLKIAEKTGLKYWEGTFCVNLSYDYHDFENYSKGIAFGKRA
                     IQIFTTLKNAKPKDIRSAFNATAINYDDWNKPEMALYYHKQNLIYLKGKDTLLLNHTY
                     NNIGNTLLKQKKFEEAKKWILRSVAILDYKVKHNDMVYIDYNYATCYTNLATIASELN
                     QYQEAQNYFDLALKYSKKSQVAEKIRDYFYQKALFNKKYNNLEATIKEQEDYIAFRDS
                     VYTSERNKTAAELEAKYQTEKKENQILQQQAESRKKNIWLLFATSILLIVGILFRNFR
                     IKSKLQKEQLELENKLLEEQSNYKMQEQRLEISRELHDNVGSQLTFIISILDNLKSSP
                     VQFEQAIDKKIDTLSNFAGKSIAELRDTIWVLNSKKLSLAELKSRMLNFIKDAGESVD
                     GTQFHFDFEVKNDVQLSSKQAINTYRILQEIVNNAIKHANATAVSVAINQNQKQLEIQ
                     ISDNGIGFDYEAKKKKSFGLTNIQNRIQEIKGDMKVQSSNKNGTIYTINIQL"
     gene            23027..23674
                     /locus_tag="PLQ70_00390"
     CDS             23027..23674
                     /locus_tag="PLQ70_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQE33103.1"
                     /translation="MIKILIVEDNFFLQKALEEKLSNFSDIIIKDIAQNGEEALAILE
                     KNHVVDLILMDIEMPKMNGIKATEIIKSKYPQIKIVIITVFDNDETIFNAIKAGADGY
                     LLKETKAEKIYEAIGETLSGGAAMSPSIAMKTLKFLKNPVVFEDLETPEQIALSDREI
                     EVLEQLSQGLKNQAIADNLFISFSTVKKHIENIYKKLQAHNRIELIQKAKQNKLL"
     gene            23748..26114
                     /locus_tag="PLQ70_00395"
     CDS             23748..26114
                     /locus_tag="PLQ70_00395"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33104.1"
                     /translation="MKKITLFILLFSAIVNAQNPLVVKDVTSSLKPTGNSDKADLNYA
                     KWNGKLFYAGSGSTMLCVTDGTTAGTIGLSALGGTTNISAIIPAQDFVYVITSDITFS
                     PSTASTDKIWKTDGTVAGTSLVYAFETATGLTNVGVYYSVPAHRKNYSVDGNKLYFSA
                     FDATNGKELWKTDGTAAGTILVKDIKPGTGSSQSSGFCKIPSTTLFIAQSVGLESKLW
                     KTDGTTSGTEQIPVAEPFYIVNQDMARLGNKVIFFAHNTVDGYEPYVSDGTAAGTFML
                     KNINPTGNSLTTQAMGLHLKMTDRYCFFIANNGTTNALWRTDGTVDGTIEVIPNVTNG
                     ISDAGYSTTDAENLWFVNYNGTNAVQQLYKSNGTVAGSSQVYSTLNFAQNLTTYKGAL
                     WFRAANSGTPANAEVWRSDGLSVNTALALDIEPTIVSGIPMSSNPNAFFELNDKLYFF
                     GKGNNNNSHYLYQYTGDFTFNGSISNDWNNKSNWNSLLKPGMTDVVTIPAGQNIEVTA
                     NAYAKNVVINGNLNLVSGNLDFSGMITLANSAKITLNNNMLNLKGAVSSLTGTNTSYV
                     ITNGTGTINVENLDATRGTISLPIGTATNYNPVSLSNSGTSDTFSARVSDGISGATNG
                     AVNATWDIAEAVVGGSNVNLSLGWNQAQENGTFDRNAAKIGHFTAGNWMQETSGTVLG
                     SNPYTLSGTGISSFSPFSVMNFSALSTSEFGIGKVAVCPNPSNGDFSIEITNEMMGSK
                     ANVYSILGQKIKTFVLNSNKTNSNLTSGIYIIEIENNGKKTIKKIIVN"
     gene            26121..27830
                     /locus_tag="PLQ70_00400"
     CDS             26121..27830
                     /locus_tag="PLQ70_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33105.1"
                     /translation="MKKKLVFSAILFCVITTSQGQFLKKITGKKETDTTKVEKKVGGG
                     LFQNIVAKVAKVAGNAAGGMAGMVSTVDNLENVDILASIGTNIYSKKLGLVVNDFLGD
                     DWIDNGDFTMIMLSSKNSFQLNKYAGSIKVNNKELKHSSYGVHTVCENPNSGNKKISF
                     EKNGIVEGSFEVPALKNNIKLLTINGQKDNVNIDLTKDVVLEFSNYSTTKESLLRIDI
                     IASVIGIRSLYLVTYVKPAAKVVIPFQAFRNIETMSKGLGFSNCYLSVSDQLAVKTIN
                     NTGFFKSPIDALTGSNDGMWINVTQKPEQFYGISIENKTKTTEVTAQKKNAAYSMPLS
                     KAQKIAVASLSIEGTTYSESSKVNAFQQTETTKTIEFPQIPDAWQEATLVEMHKNITA
                     TTGQILGGTILPVEIVTQAPSYNEVQNFFREETNTSSNFLRAYKGLNPIKPLSTSSLK
                     MYGENALLSETKSDALLKIKMVLELSWDSKPLMTPFLYVTMDGASNGAFRSASGNTNY
                     FTIKIKGEPYEIKKGKVITPDEYVKITQVALLNQIYKETLSSLKQKELEKGDYEVIWN
                     LQK"
     gene            27890..28510
                     /locus_tag="PLQ70_00405"
     CDS             27890..28510
                     /locus_tag="PLQ70_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HQE33106.1"
                     /translation="MQRRILIGAGIIVAIILLFKFCDFKKEDDSTVDYNTNLIQQQIV
                     NVGKLVVTEGHFAEVITYKDQDKYLMDMVSFEKKAIVIVNADVTVAYDLHQMKYDIDE
                     KNKTITIKNIPKEEIKISPDIKYYDVQQSQMNPFTGDDYNKINKIVRANLAKKIEKSS
                     LKTNAQNRLISELSKMLILTNTMGWKLQYEGKVIEKEADFNQKIKG"
     gene            complement(28507..>29672)
                     /locus_tag="PLQ70_00410"
     CDS             complement(28507..>29672)
                     /locus_tag="PLQ70_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQE33107.1"
                     /translation="IRHSQGWINEIIENLDELVKRVNLAKANKEIVSIAYLGNVVEVW
                     EKFDQENIFIDLGSDQTSLHNPWAGGYYPVGISFEESNEMMANNPDLFKEKVQESLRR
                     HTNAINKHTAKGTYFFDYGNAFLLEASRAGAAIMAENGIDFKYPSYVQDIMGPMCFDY
                     GFGPFRWVCASGKPEDLEKTDRIACEVLEEMMQHSPAGIQQQMADNIQWIKGAQENKL
                     VVGSQARILYADAEGRMKIAEAFNQAIAKGEIGTVVLGRDHHDVSGTDSPYRETSNIY
                     DGSRFTADMAIQNVIGDSFRGATWVSIHNGGGVGWGEVINGGFGMVLDGSREASKRIE
                     SMLFWDVNNGISRRSWARNEGAIFAIKRAMETQPLLKVTLPNLVNDDLFEDLN"
BASE COUNT        10471 a         4882 c         5368 g         8951 t
ORIGIN      
        1 tatgaactct aatctcgcca gaagtttctt tttcagccat acgaatagct tcaacgattg
       61 cttgttcttc ttctttggtt aaaaaatctt caacttttga catttttata tttttttaaa
      121 gtccctcctt tggaggggtt ggggaggatt tagaatttta cttcaacagg tttatcggcg
      181 ccttcaaccg ctttgaaata tggtttttct ttgtattttc ctaagaagat actttgtggc
      241 attgccaata cataaccatt gtaatcttga actgattcgt taaaacgagt tcttgcagta
      301 agaatttgat tttcggtact tgccaattcg tcttgtaatt tcaagaagtt ttcattggct
      361 ttcaaagttg gatattgctc cacactaact aataatctag ataaagaaga agaaactcca
      421 gattgagcag aattaaaagc tgccaattgt tctggagtaa cgtttgcagg gtcaatagtt
      481 actgaagttg cttttgctct cgcttcgatt acggcagtca aagtactttt ttcgaaatcg
      541 gcagcacctt taacggtgtt tactaaattt ccaataaggt catttcgtct ttggtaggct
      601 gtgtttacat ttccccattc tttattgaca gcctgatctt tttgcaagcc tgtgttcata
      661 attcccatta cccaaaaagc aatgattaag ataaatccaa ttccaacaat ccaaggtaaa
      721 aattttttaa aatccatttt tgtttaattt aaggtttagt ttttatttat tgtttttaaa
      781 ttctgctttc taaattctac aattcattct taatattagt taattgcact ttaattgctt
      841 ccaatttact gataatatca aatttatcca aagttttctt ttgtccttcc ttcaaatgtg
      901 ttcgggcgcc ttcgagcgta aaacctcttt ctttaactaa atgataaatc aattgcaggt
      961 tttttacatc ttctggtgta aacattcggt tgcctttggc atttttcttg ggtttgagaa
     1021 tgtcaaattc actatcccaa aaacgaatga gcgaggcatt cacatcaaaa gctttggcta
     1081 cttcgccaat gctgtaatat cttttgtctg ccgataattc tatgtgcata ttttatagct
     1141 ttttctatgt tcttttctat attcttttct ctcttttcga cttaatcaaa cgactgattc
     1201 tcctgattcg ctgcttgtgc aatggccaca tattctacag ccgaaatgtt tccgtaataa
     1261 aagttcagcg gattcacaac tttaccattt ttatgcactt cataatgcaa atgtggtcct
     1321 tctgatcttc cggtgcttcc tacataacct atcacgtcgc cacgtttaat actttgtcct
     1381 gccctgcatt tgtatttgct aagatgacca tataaagttt cataaccaaa tccgtgtcta
     1441 attacaatat gatttccata tcccgaagct gtattatcag ctttagcaac aacgccatca
     1501 cctgtggcat aaataggagt tcccgtttta gccgtaaaat ccattccttc gtgcattttt
     1561 ctggctttgg taaaagggtc agttcgatat ccaaaacccg aaaccatact ttttaaattt
     1621 tcattcttca ccggttgaat tgctggaatg gctgctaata atttatcttt tgccttggcc
     1681 attttcaaaa tgtcatccaa tgattttgac tgaatggcta gctcttttcg caacacatca
     1741 attcttttta tcgtattggt taccaactgc gaattgtcat aaccttctaa atctttatag
     1801 cgattaacct ctttaaatcc cgatttcctt tcttcttctg aaataggtga cgcattaaaa
     1861 taaaccctat ataaattgtt gtctctttct tctataatct caattacatc ctcgacttcc
     1921 tccatttttt tgttcaaaat agcatatcgc aatttcaaat tctcgatttc gcgagcctgc
     1981 tttttatttt ttggagtttc aaaataaggt gtattcaata aaataatgaa acttaaaaca
     2041 ccaaacaatc ccgaggctac taaaaacaat aaaacaacgc caattttctt cctggttttg
     2101 gttattatct ttcgataggc tagattttcg gaatcgtaat aatattttac tttcgccata
     2161 ttttaaaata ccctattttt gcacatcgta aaaaggttag tcgaacaaat ttaataaatg
     2221 tttcattttt tcaacccttt tttcgataat aaataaatta atatgtaaat gtctaaatgt
     2281 aacaatttgt aaattgatgc ataggtacat ttatacattt tctaattaca attatgaaat
     2341 cacaagacgt acgcaaacaa tttttagatt ttttcgaaag caaaggccat ttaatagttc
     2401 cttcagctcc aattgttctt aagaacgacc caactctgat gttcaacaac tcaggaatgg
     2461 cgcaattcaa agaatatttt ttgggcaacg ccacaccaaa aagcaaaaga attgccgata
     2521 cgcaaaaatg tcttcgtgtt tcaggaaagc ataatgacct tgaagaagta ggaatcgata
     2581 cctatcatca tacgatgttc gaaatgctgg gcaactggtc ctttggcgat tatttcaaaa
     2641 aagaagccat caactgggcg tgggaattgt tgaccgaagt gtataaaata ccaaaagaca
     2701 ttctttacgt ttctgttttt gaaggaaatg ccgaagaaaa cgtgcctttc gatcaagaag
     2761 cctatgatat ttggaaaact ttaatcgatg aagaccgaat tattcttgga aataaaaaag
     2821 acaatttctg ggaaatggga gatcaaggac cttgtggacc ttgttcagaa attcacgtgg
     2881 acattcgttc tgccgaagaa aaagctttgg tttcaggaaa atctttagtg aataatgacc
     2941 atcctcacgt ggtggagatt tggaacaatg tatttatgga attcaaccgt aaagccgatg
     3001 gttctctaga aaaattacca gcacaacacg tcgatactgg aatgggattc gaaagattgt
     3061 gtatggtttt gcaaggtgtt caatcgaatt atgataccga tgtttttaca ccattaatta
     3121 gagaaatcga attaattaca ggagctaaat acaccatcaa agcttctaac gaagaggaag
     3181 aaaaaataaa tattgctatt cgtgtcattg ccgatcacgt tcgtgcagta gctttctcta
     3241 ttgcagatgg tcaattgcca tcgaatactg gagcgggtta tgtgattcgt agaattttgc
     3301 gtcgtgcaat tcgttatggc tttacttttt taaataccaa agagcctttt atttacaaat
     3361 tagtagagac tttgagtgct caaatggggg attctttccc agaaataaaa tcacaaaaaa
     3421 cactttgtag caacgtgatt cgtgaagaag aaaattcttt tttaagaaca ctggatcaag
     3481 gtttggtgct attagaaaat ataattacca attcaaaaga tactgttatt tcaggtaaaa
     3541 aagcttttga attgtttgat acttatggtt ttccaattga tttaacggct ttgattcttt
     3601 cggaaaaagg attgagttta gacgaagcgg cttttgaatc ggaattgcaa aaacaaaaag
     3661 aacgttctcg tgcggcatcc aaagtgaaag cgggagattg gcaagattta attgatgacg
     3721 aagagcaaga gtttataggt tacgattata ccgaagccaa agtaaaaatc actcgttaca
     3781 gaaaagtaga aagtgtaaaa gatggcgaaa ttttccaatt ggttttcaat atgacgcctt
     3841 tttatccaga aggtggtggt caagttggcg atgcgggagt tttggaaacg gctaatggcg
     3901 atgtgattta tatccttgat accaaaaaag aaaacaacca aattattcat tttacgaaag
     3961 aattgccagc aaatcttaca gaaattttta aagtaattgt agctccagat cgcagaagaa
     4021 aatcggctgc caatcactcg gcgacgcatt tgatgcacca agctttgcgt tctattcttg
     4081 gaactcacgt agagcaaaaa ggatccttag taaattctaa taatttgcgt ttcgatttct
     4141 cgcatttctc gaaaatgaca gacgatgaat tgaaacaagt agaagacttt gtaaatgcta
     4201 gaatccaaga gcaattgcct ttgattgaaa gaagaaatat tccttttcag caagcggttg
     4261 acgaaggagc gatggccttg tttggcgaaa aatatgggga tgctgttcgt gccatcaaat
     4321 ttggcgaaag tatggaattg tgtggtggaa ttcacgtagg caacacagct gatatttggt
     4381 atttcaaaat tgtttcagaa ggagcagttg cagcaggaat tcgtcgtatt gaagcgatta
     4441 ctagcgatgc ggtgcaaacc tatttcgaaa cccaagaaaa aattctagat gaggttaaaa
     4501 ccattttgaa aaatccacaa gatgttatca aatcagttca ttcgttgcaa gacgagaatg
     4561 ccaaattgaa aaaacaattg gaagttttat tgaaagataa ggccaaaaat atgaaaggcg
     4621 aattagcaca agaactgcaa gaaataaacg gagtacaatt cttagcaaaa caagttgatt
     4681 taagtccaga aggagcaaaa gatttggctt acgaattagg aactttaggc aaaaatattt
     4741 tcctagtttt ggctacagcc gaagaaggta aaccaatgct aagctgttat atttccaaag
     4801 aattggttgc tgagaaaaac ctaaacgcag gtcaagttgt tcgtgaatta ggtaaattta
     4861 tacaaggtgg aggcggagga caacctttct ttgctactgc aggagggaaa aatgttgatg
     4921 gaattccaga ggctttggca aaggcagttg actttgtgaa gtagtatttg aaatagttct
     4981 aaaaaaaggg tttgactttt aaatcaaacc cttttttatt ttgaaatcaa attatttttt
     5041 aagagcccta cctttttgag catttacagc atctcgatag agtttatatc tgtcatcacc
     5101 taataattct cttagtcttg catttttgat tttagcatat acctttaatt tcataatttt
     5161 ttcctcttcg gtaaaatcag gatcagctct taattctttt ccgtattcgc cactctcttt
     5221 tatgaattct ctcattttgg cttgttcttc gggtgtagcc tgaattattt tgaaaacttc
     5281 taaaagattg gcttcttttt tggctttggc agcttctttt tcttctttgg atacggtttt
     5341 ttcttgaggg gctttttctg cctctttttc ttgtgctaca gccgccgtgt aggagaggca
     5401 tactgcgaac aatgttgtta ataagtgttt cattttttta ggtttgggga ttactaaaat
     5461 taattttaaa aaactaagtt gcaaatataa atattaatgt aagtcaatgg ctgaaattat
     5521 gatttttaac tcaatatttt taatattcga aaggaaataa agctcgaata aatggctcta
     5581 ttttttaata ttgatgtcta attatccaat gaaatggagc gaaaattgtc ttgactttcg
     5641 atttttgaaa gtagtttaac cgaaatatta ggtttgtaac ggagaatttt ggaggcattc
     5701 tacttataaa gttgatttgc attccaaatt agagtcttgg ttttttgaaa gacaaaagga
     5761 tttaaaaatt tatatgaata aagcaaaaaa aatagttggt gttctagttg ttgttttaat
     5821 gacgctttgt tcttgcacaa ccgaaactgt tgaacaaaca tcattcttaa aaaagatagt
     5881 tgaagtttca gttgatggca cttcaaacac cacaaatctt atttacgacg gaaataaaat
     5941 tctaactatt gacaaagtgg accaactttt gaaattttat tataccggca atttcattac
     6001 aaaagttgtg gaattagata aaactacgca acacaccaat acattagact attcttattc
     6061 tgtggagggt caattgacta aaataatttc ctcagataat tacctaataa attatgttca
     6121 caatagtgac ggttcagttt cttatgagaa attgaccaaa gattctacta agcctgaaat
     6181 taaaaattat cacggaattt tgtattttca aaacggaaat ctagtgaaag atgataaaac
     6241 tcaggatgat gcaggatcag ggattttgat taaaaacaca atcaacattc aatatgatta
     6301 caaaaataat gcattgaaaa atattttggg ttttactaaa ttattagatt actcaagatt
     6361 agtttctact aataatgagg ttgttagtac agaggcttac tcggttaagg atgttgataa
     6421 cgaccaaatt acttcttcca taaaacgaat cgatagccaa tgccaataca attcaaatgg
     6481 gtatccaacc gaaattgtct ctgaaaaaat actatttggt ggttctgatt ccaaccattt
     6541 aaagtcgcaa ttattctata attagttcta tttttgtaaa aaataataga tgcaattcag
     6601 aactaaaatt tctattcctc aaagcgattt tccaatagat tacgattcta aaattctgtc
     6661 tttgggttct tgttttgcag tgaatatggc ggagaaattc gattatttta agttcgaaaa
     6721 cacctgtaat cctgttggga ttttatttca cccattagca atcgaaaaac taatagattt
     6781 tgccgtttct ggaaaacaat tttgcgcgga agacgttttt tttcataatg aacgttggca
     6841 ttgtttcgat gtgcattccg attgcagtca ttcaaataaa gatgaattat tgctgaattt
     6901 aaacacaatt ctcgaaacga caaggctgca aataaccgaa tccacacacg ttataattac
     6961 ctacggaact tcgtgggtgt atcgaaatat agcaaccaaa gaaattgtgg cgaattgcca
     7021 taaagtgccg caaaagcaat ttggaaagga gcttttatcg gttggtgcta ttgaaaaatc
     7081 catccaaaac actttagatt taatccagaa agtgaaccca aaagcgacga ttattttcac
     7141 ggtttcgcca gttcgtcacc tcaaagacgg ttttgtagaa aaccaacaaa gtaaagcgca
     7201 tttgattaca gcaatccata cagccatcaa ccaacaacta tcaactaaca actatttccc
     7261 ttcttacgaa ataatgatgg acgaacttcg tgattatcgt ttttatgccg aagatatgat
     7321 tcatccgagt caggtggcga ttgattatat ttggcaacgg ttttcagaaa cttccatttc
     7381 tgaggaaagt ctttctgtta tgaaagaagt cgaaaccatt caaaaaggat tgcagcaccg
     7441 accattcaat cccaattccg aaagtcatca gcaattttta tccaaacttc aagataaaat
     7501 gatgcaaatt caaaaacagt ttcctcaaat tcagttttaa aaaatacgta attcttcgta
     7561 ttgattctat tacataattg atgcagattt gtaaagtact aaccaacaaa tcaaatcatt
     7621 atgaaaaaaa accaacttat attatttctt gggctttttc tcttactatt ttctgaacca
     7681 gtttttgcac agaaaaaagt gttgaccgac gaagaagaag tgaccgaaat ggtgaccaag
     7741 gaaatggatg aacttttcaa agacaaggat ttccttaaac aaaaagacaa aaaatttaag
     7801 gatgtcaaag gaacaattgt ctcagacatt agcgtgattc aaagcggaaa agtgtccaca
     7861 cttttcaagg tcgatagcga tatcaacaat ccttcgttta ctaattttct ttcagattat
     7921 atcatcaatt ataaattcaa attcaaacta caaaaacaac aacgttataa aattcgatac
     7981 actgtaactt tttaaataat taactaaaaa cgcaaaaaaa tgaagaaact aacacttttg
     8041 atgttgagca tctgtatgat ggcaacagtg ccaacttcgg cacaattcgg caaacttttg
     8101 gataaagcca aaaatgcagt ttctggcagc ggtgccggaa aaaaaacagg aagctttgcc
     8161 actgtttggg aatcagaatt cggaaacaaa gctacaagat tggctgtttg tggaggcact
     8221 ggcgatttta ttattggcac agacgataat tcggcctctg ttttagactc ctctggaaaa
     8281 gcaatttgga atggcgatta caaaaaaatc actactaatg gcaccaataa atcggaatac
     8341 caatacaccc aatggaaatc ggataaagga ggctacttat tcctttttga tgaacgcaaa
     8401 ttaggaaccg atagagtggc ttgtttagac attgaaacag gaaaggaatt atggagttct
     8461 gaatcctatc aaaatttaat tccaaaaggg actaaaagtg aagatggggc cgatcaaggc
     8521 gaactggata ccgtaaaata catctcggaa ctggatgctt ttttaatttc tcaaaaagct
     8581 tccatcattc ttgtgaaagc caatacagga gaaaaaattt gggaaaccaa tagatttaag
     8641 ggtggagttg gtaaatacat ttacaacaaa gaaaaaaacg aaatcattat ggccaatttc
     8701 aaaccaactg ctttgggggc tttgtttgca ggtttcaaaa accaattggt tagagtcaat
     8761 gcatcaaatg gcgaaatttt gtgggacgcc acctttgtgg gaacagtcga aaaagagttg
     8821 gttacccgca gagccatact tgatttgtgg atcagagaca acaaaatttt cttgtatctg
     8881 aacggtctta tggtttatga ttacaataat gggcaaaaac tttgggaggc catttatgaa
     8941 aatgatatga gtggcggagg cggaggaatg ttctctaaca cacaaagcaa aaaatattac
     9001 agaactattg cagatccttt atttacagag aatgcagttt atatcgtgat gttaggaaca
     9061 agagacagaa caaaatatgt agaaaaacac gatttggatt cgggtaaatt gttgtgggca
     9121 tccgagaaag tgactggggc attttgcgtt ccaaatattt acaaaactgg tgataaagtt
     9181 ttgatacaaa ttggcggaaa agtgcaagtt caggaatatc gaatggaaac tcaaacttcg
     9241 ccatttggtg gaacttacaa agcaagcgta ccctacattt attgggatta taaaaatcaa
     9301 aagaacggag tactttgtat ggatgacaca agcggaaaat tggcttggcg ttcagataaa
     9361 ttcgacaaac gaattaccga tttgattata aacgaagaaa aaacggtttt tgttggggat
     9421 ggcgatgagt tttatagcta tgaaattgcc tctgggaaac aattattcga cgtaaaacac
     9481 aatgatgctg gcgttggaaa agctaccgat gttattgact ttgaagacaa agttgtggtg
     9541 ctttcagaaa aaggattggc atcctacaac aagaaagatg ggtcaagagc ttatgctacc
     9601 gaaaaaataa aaggggtaga ttatttttat cacattggcg acaattattt cttgagagat
     9661 caaagaaaca gcaaaaacat catctacgga atcgatatga ccaatggcga gaccaaaggt
     9721 tctgtgcaat ctaaaggaaa aggaggaagc ccgacttatg gtgatggaat cgatattacc
     9781 tcggatggcg aatatatttt tgctttcaaa ggcagaaaag tagaaaaaat caaggtaaat
     9841 aattaagcgt tttttagtta gaatgaaaag ggttgtgaat tgattttcat agcccttttc
     9901 taaataaatt ccactaaaaa tttatgttta ataaccttaa attccttaat ggtttaaaaa
     9961 tagtaatatg aaaaaaacaa tatttctttg ttctctattc tttgttgttt tttcaataaa
    10021 agcccaacaa atccaatggg ccagcaaatt gattaaattc tcgaccgatt taggaggaaa
    10081 acaaaacggc atcaaaagaa ttctgggcaa acccgatgct tttccgcaag gaggaagttc
    10141 cgccaatgct tggatgccaa aaaatgccct cgacggaaga gaaataatcg aagtaggttt
    10201 cgaaaaacca caaacggtaa aacaagtcgc tgtttgcgaa aacttgaatt cgggttgcgt
    10261 aatcaaaatt tcggtggata atggttcagg aaattatgaa acggtttgga cacgaaaaag
    10321 agattggaaa acacccactt tcaaatccac ggcatctgca gaccacgctt attattttgg
    10381 tcgaaaaagg agaaaagttc aggaagtgcc caacgttttc aatcccggaa ttgaatatgc
    10441 cgttttggaa aatgctgttg ccaacgtagt tgcaataaaa gtggaattca attttgcttt
    10501 gcttccaggt ccaaaacaaa ttgacgccat cggaatttcc gattcagaga aacctatcga
    10561 aataaaaatc aattctaatt ctgctttcga aaatttgccc aatccagaat ctttggagtt
    10621 cgataacagc gaagtttcta atgtgatagt ttcacaagac ggacaaaaaa ttttctattc
    10681 cgcttttgcc gaaaataaag atgtagtttt ttcgagtcaa aaacaagccg atgggaaatg
    10741 gtcaaaacct gttgtagaac caagtttgag tttgaacaat gactacaatt atctcgaatt
    10801 ttacagcgat acttttatct taaaaggagg cgtgggatat tctaaaaaca ctaccgaaac
    10861 tggatttgaa tttattgaaa acaagaacgg aagttatcaa agtctaaaac cactgaaaat
    10921 tgccgcctat aacaattacg aaaaaacggc agacatcact atcacaaacg acggcaaaat
    10981 cttgattatg ggaatcgaaa ccgactttac ccaaggcgga accgatttgt attttgccaa
    11041 ccgaaaagaa gatggcactt atggtttgtt gcaaaatatg ggtaaaatca tcaattcagc
    11101 tgccgatgag ggaatgccac agttattgtc agacacaaaa acactgtttt ttagcagcat
    11161 tggtttcagt agctacggga attatgatct ttatgtttcc tacagattgg acgacagttg
    11221 gaaaaaatgg tcggaaccca ttaatcttgg aaataaaata aacagcgaca gtttcgaggg
    11281 ttccccattt tatgacgaaa ctactgaaat gctttatttt accagagttg ttgaaggtaa
    11341 atcgtctatt agtcgggtga aaattccgaa aaaagaactg gtgaaaaatt gatttttaat
    11401 actttaaaaa tacgataaat gtcgtatttt tttgtgttta ctaatttcgg aactttgatt
    11461 gataactttt aaatagcacc aagatgaaaa aaaaaatctt cttttagtga tgtgcatact
    11521 ttcttattct gctagttttt cacaaattag cctagtaaaa gaaatttacg caggggacag
    11581 ttattatcct ccaaaagagt tgttggaatt aaatggcaga cttttgttta caaccgatgc
    11641 accagcaggt tcggtgtcta gtttttggaa atcagacgga acattggcgg gaactacaat
    11701 tctgggaacc tttggcccag accaaagagt cagtttctca aaaaagttta ataatttcat
    11761 ctattttaat gttggaaaaa atggagcagt ccagtcttta tgggttaccg atgggaatgg
    11821 ggtgtatttg gtaaagtcat taggctccac ttcggctaca ggagaattga ctgtattcaa
    11881 gaacgccttg tattttaata catcatcatt atttgcacag ggaattcata aaagtgacgg
    11941 aactgcggca gggactgttg gatttgcaaa tattacggct acaagtcctt tggtagtcag
    12001 cggaaccaat atgtatttta ctacttatgt agccagtgtt ggaaatgaac tatggaaaac
    12061 agatggtacg gttgctggaa cggttatggt caaagatatt tatccaggta gtgttgatgc
    12121 tcaaatatct caaatgacgg acatcaatgg aactttgttt tttatagcaa acaatggagt
    12181 taatggaaat gaattgtgga aaagtgatgg aacagctgca ggaacggtga tggtaaaaga
    12241 cattacagca ggaggaattt attcacattc gattctaaat tatttgacca atgtaaatgg
    12301 aatactgtat tttacaaaag ggaattatga attatggaaa agtgatggta cagatgcagg
    12361 aacggttttg gttagacctt caacgtccat atcgcctacg aatttaatta atgtaaatgg
    12421 aacgttgttt tttgaatgtg gtacgctttc aacgggtgcc gaaatttgta aaaccgacgg
    12481 gacaacttcg ggtacagtaa tggtaaagga cattaatgta agcggaacag gttatggtgg
    12541 attcataaat ccgtatgtgg tgggccaaac ggtttatttt agagcacaaa acggaagcaa
    12601 tggttatgaa ttatggaaaa gtgatggcac cgaagcaggc acacaaatgg tttatgacac
    12661 caatgtgggt tcggccaatt ttaatccaga aaagttaacc aaagctggaa acaaactttt
    12721 tatggtgggg caaaccgctg ccgagggaat ggagcttttt gtatataaca ttccgagcct
    12781 ttctagcaat accatcgacg gtataactcc attattagtt tatccaaacc cttcaaaagg
    12841 gaatttcaac atcgaaattg atgaaaatct aattggtgca aaagctagtg ttttcagtct
    12901 tttgggtcaa aaaataaagg atttcgaatt aaaatctaca attacaaatc aatttttaaa
    12961 caaaggaatt tatcttttag aaattgaaaa agcaggtaac aagacttcta agaaactgat
    13021 tgttgaataa ctttttgaaa aatagataac caaaaaaata cgaaaaactt cgtatttttt
    13081 tggttatggc cactgcttat ttttgattta acaatttata aaatcattca aaatggaaaa
    13141 acaactactc ttttttgtat tctttttatg cattggcaaa agttcatttg cccaatacac
    13201 caaactatta gattttgacg gtactgccaa aggcaaatat ccttacggga cattgtattc
    13261 cgatggcatt tttctgtatg gaacgacaag cattggcgga acaaacgatt tgggaacagt
    13321 gttcaaaatt aaacctgatg gaacagggtt ttcaaaacta ttggattttg caggaaccac
    13381 aaacggaagc aatccttacg gcaatcttat ttcggacggg acttttcttt acggaatgac
    13441 ggtcaatggt ggcgctaata atttgggaac cattttcaaa ataatgcccg atgggacagg
    13501 ctacgcaaaa ctgttggact ttgcaggaac atctaatggt ggcaatcctt ggggttcgct
    13561 tatttctgat ggaacttatc tttacggaat gacctttgtg ggaggttcaa acagtatggg
    13621 aaccgttttt aaaataaagt ttgacggaac tggattcaca aaattattag attttgcggg
    13681 attatctaac ggaagaaatc ctcggggaga tctcttttat gacggaactt acctttatgg
    13741 aatgactagt gttggtggtt cggataattt cggagtcctg ttcaaaatgc tgcccaatgg
    13801 aactggatat actaagctcc taaactttga cagtgttacc aatggaagaa atccacgtgg
    13861 taacctaatt ttcgatggca cttatcttta tggaactact tcgggtagtg gaacaaactc
    13921 atctacaaat tttggaacta ttttcaaaat aatgccaaat ggaacaggat atgtaaaatt
    13981 gctggaattt gatggtgtca caaaagggag atatccttat gggtcgttac tatcagacgg
    14041 aacctatcta tacggaatga gctacaatgg tggtattgac aattttggaa cgctgtataa
    14101 aattatgctt aatggaacag ggtttacaaa attaatagat tttaacatca caaacggtca
    14161 aattccttgg ggttcgctta ttgctgtagg ttcatccctt tatggagtga ctgctggagg
    14221 tggtacggct ttatcgggag tactttttaa atacggaatt tcaactttaa gtgttgccaa
    14281 aaatcaaaag ccaaatgatt tcaaaattta tccaaatcct tccaatggaa attttaatat
    14341 tgagattgac gaaaatttaa taggctcaaa agctacaatt ttcaatcttt taggtcaaaa
    14401 aataaaagat ttcgatttga aaactaccac tacaaccgag tttttaaaca aaggaattta
    14461 tcttctggaa atcgagaaag atggaagtaa aaccacccaa aaactgattg ttaattaggg
    14521 ttgatctcgg attttaaaaa caaaaaaata cgacattttt cgtatttttt tgttaataca
    14581 cccttcttat ttttgaagaa taatttttaa aactacaaag atgaaaagaa taattactat
    14641 actattaatt gtgtttttta ccacaattac aaatgcgcaa tggacattgt gtaataatcc
    14701 aagtggtgcg ctaccttttt cattaacaat tcagaatgat accctgtatc taggaacttt
    14761 aagcaatggt atttatcgtt caacaaatgg agggtccagt tggacacaaa taaacaacag
    14821 tattacaagt atgcaaattt ggaatatagc ctatgtcaat ggctcccttt ttgccagttc
    14881 tactaacgga acggttttca agtcaacaaa tggtggagat agttggattt tgtctaatac
    14941 cggaattagc acgaccacaa taattaggaa ttttgccttt ttcaacaata agatatttgc
    15001 cacttcaacc aataaagggg tttatatctc ttctgacaac ggaagttctt ggacacaaca
    15061 caatactggg cttgtaggtt tggtagccga accattgcta gtagtggata cggatttatt
    15121 tgtaggagta aatcaaaagg tgtacaaata cgatagtgta aatcaaaatt ggattagtaa
    15181 aagtgttgga attccaaaca ataccgttac ctccttgact tacacaaaag acaatctgca
    15241 aaacataagc cttttcgaat cgaccgtcaa taccaatgat gtcacaatat caacaaataa
    15301 aggtgatagt tggagtgtcg ttaagaatgg tcttcctagt gtaggggttt attcgcttct
    15361 tggcattgga acagaggtgt atttaggaaa tgattatggg gtttataaaa ctactaatca
    15421 aggaacgaat tgggttgata tttcaggatt tacaaatgcc agtcctgcca aatttcttag
    15481 caaaagttca acggatttgt atgtcattca agaagcaaaa atttggaaaa aaagtttgaa
    15541 taatttaggt gtttcgggtc tggaaaatgg ctttgaaagt caaaccataa aaatttatcc
    15601 caacccaact tcaggaaatt tcaacatcga aattgatgaa aatttaatag gtgccaaagc
    15661 gaccatttac aatcttttag gacaaaaaat aaaaacttta gatttgaaaa ccaccactac
    15721 aaccgaggtt ttaaataaag gaatttatct tttggaaatc gaaaaagacg gaaataaaac
    15781 cacccaaaaa ctgattgtta attaaaattc agtaaggcta tgccaataga aatttcaaat
    15841 gcaataattg taattctgac gaccctgatt ttggtactta tttttgtaat cataaaattg
    15901 aacattcagt tttatagaga aaaaaaaagc ttcaaaaaaa aaatgaaagt tttgggggag
    15961 attatcgttc aaattagtag agataaatca gggaaaggca atcaaatcag gctttcagac
    16021 gaattgaata gtaaattaaa ggcattcaat agcgctttag ggtctgatat tttcgaactc
    16081 aataaagaat tattcgaaat tttgtccaaa aacaatttgg cttaaaaatg ctgtaattga
    16141 tttattatta agttgggtag ttgttttttt tgttgtagct aaagtttttt tattacattt
    16201 atttttcaat ctactaatac cttttccaat tgaagataac caagattaag atagttgaat
    16261 cgatgccttc atagtaaaaa aggtaatgtg gctattacaa ttcttggcat cctaattggt
    16321 gtagagaata ctgtctgttt ctggcagcta ataaaattaa taggcctatg aaaatagcta
    16381 gactttttct ttttttacta ctttgtttag ttcagttttc gtattcccaa gaggccaaaa
    16441 agttaattta taaagctttt gggtcaaaag attcttcgga ttattatttt aaattggcta
    16501 aaaaaaacat caaaactccc gcagatgaag ctgaatatta ctttggtaaa aatggccgat
    16561 gcaatgatta taatcagccc gacagctgcg tcttttatgg gctgattgcg attcagaaat
    16621 ttaaaaaaat tggaaatagc aattctctgt attatgtcta taataatatt gcagcaagtt
    16681 ataggaaaca aggacagtac gaaaaagcca ttagttatat tctggatgga ttgaaacttg
    16741 ccgaaactga aaataaagaa tattggctcg caaattacaa ctcaaatatt tccttaaatt
    16801 atcacgattt cgaaagttat caaaaaggga ttttgtatgg aaaaaaagct ttgaattatg
    16861 gtttaaaagc caaaaataga gataaaaaat caatcacttt tgctttaaat gctattgcaa
    16921 taaattttga tgattggaat aagcctgata gtgcattata ttatcacaaa aaagttttta
    16981 actatgtaaa aggcaaagac actcttgatt acagtcatac ttacaacaat attggcaata
    17041 cttatatgaa acaaaaaaag tataaagaag ccgaaagttg gattaaaaga gcaatgatta
    17101 ttggagcaac aaattcaaat ggagtaaaag acgctagtta ttactatgaa aacgcaactc
    17161 attacaccaa tttagcaaca atagcataca atttagatga ttttgctaaa gctgaaactt
    17221 tatttgacaa agcctatttt tttgtaaaaa acagcaaatc agccgaaaaa tttcgggatt
    17281 attttcagca aagatatttg ttcaataaaa aacgaaaaaa cctcgaaaaa acggtcgaat
    17341 ttcaagaaaa ttatattaaa ctcagagatt cagtttttga attagaaaga gcaaaaacct
    17401 ttaccgaact cgaaacgaaa taccaaaccg ccaaaaaaga aaaactatta atagaaaaag
    17461 aagccgaagc caaacaaaaa aatacaatgc tcataggaat ttcgctttta gcttttttta
    17521 tagcacttat aggtttttta atctaccgcc aacaaaaaca aaaaaatgta caacaaaagc
    17581 aagaatacaa actcaaaaaa gaaatttcta aaatagaaac tcataacaaa ttgcaagagc
    17641 agcgacttag tatttcaaga gatttgcacg ataatattgg agcacaatta acttttatta
    17701 tttcatcagt tgataatatt aagtatgctt ttgacattac caacgaaaaa ttagaccata
    17761 aactttctca tatttctaat tttgccaaag aaaccatttt ggaacttcgg gacacgattt
    17821 gggcaatgaa tagcaacgaa atcacctttg aagatttaga aatcagaatc aataattata
    17881 tcgaaaaggc aaaagaagct aaagaccaaa tcagtttttc gtttgccatt gatccagaat
    17941 tgaaaaccca aaaattgacc tcggttcaag gaatgaatat ttacaggacc attcaagagg
    18001 cggttaataa ttccataaaa tatgccaacg caagtattat ttccattaat gcaaaaaaaa
    18061 tcgaaaaccg aataaaaatc agtattcagg acaacggaat tggttttgat ttggttactg
    18121 tcgaaaaagg gaatggtttg aaaaacatgc aaaagcgaat cgaagaaatt ggaggagagt
    18181 ttcatctttc ttcctcgaat gaaggaacac taattgaagt aatattataa acaatgaaaa
    18241 aaaaattact gctacttctc ttaattacta caacagcttt ttctcaaaat gctcaagaaa
    18301 ttattgatgg actaaagaaa gatttgaaaa acagccctga tgcaaaaaaa acggccacaa
    18361 tttattcgga tttgacttgg tattattcta ctgtttccat cgattcagct ttgcactatg
    18421 gaaataaagc agttatagct tcaacaaaac tgggagattc gaccttaata gctcaagtgt
    18481 ttagtgatgt tggagcggtg tattttagaa aaggagattt tcaaaattca aaggaaaatt
    18541 acctcaaagc ttatagaatc agaaaagcta gaaatgatta tagaggtttg gctaaaatca
    18601 ataataattt ggctaatatt tacgaaaaaa atggtcaata taaacaagca atgacttcgt
    18661 ttcttgacgc tttgcaattt tttgaaagta taaaagacga aaagaacagt catattacca
    18721 aaggaaacat tggactcata cttttaaaat taaaaaatta tccaaaagct tttacctata
    18781 taaatgacgt tgttgaatat caggaagaaa acaatgctac ggcagaatta tgtgtttcct
    18841 gtttgaattt aggcaatgtg tatcttcaaa tgaatgatac tcttaacgca ttaaaacagt
    18901 ataataaaag tttgaaatat tgtaataatg ttggaaataa aaaaggagtt tctagtgctt
    18961 ataacaatat tgcatctata aaaacagagc aaaaaaaatc taaagaagcg ctggtacttt
    19021 atgcaaaatc acagcaagtt agaaagcaat taaactcgga tattgacaaa gcaaatttcg
    19081 acttaaatgt tgctgaagaa tttatgcatc acaataaatt gaacgaagca aaacaattat
    19141 tgttgaacac caaacatgtt tttgaaaaag aaaaattata cgacaaatta caaataaatt
    19201 ataagtcgct aattcgggtt tatgccaaat tgaaccaatt ggacagtgtt agtatctatg
    19261 tagataaatt gttagtaatt aacaatcaat tacttgaaaa ttcagttgta aagcaaactg
    19321 ctgaactcga aacgaaatac caaaccgaaa agaaagaaaa actcttactc gaaaaagaag
    19381 tcgaagccaa acaaaaaaac acactcatac ttgggatttc ggttttagct ttttttatcg
    19441 ctctaattgg ttacttgatt taccgccaac aaaaacaaaa aaatatccaa caagctcaag
    19501 aatacaagct caaaaaggcg atcattaaaa ttgaaaacca aaacaaattg caagagcagc
    19561 gcttgaatat atccagagat ttgcacgata atattggtgc tcaattgact ttcataatat
    19621 catcggtcga taatatcaag tatgcttttg acattaccaa cgaaaaacta gacaacaaac
    19681 tttctaatat ttccagtttt gccaaagaaa ccattctgga acttcgagac acgatttggg
    19741 caatgaacag caacgaaatt accttcgaag atttagaaat cagaatcaat aattatatcg
    19801 aaaaggccaa agaagccaaa gaccaaatca gtttttcctt tgccattgac ccagaattga
    19861 aaacccaaaa attgacctcg gttcaaggaa tgaatattta tagaaccatt caggaagcgg
    19921 ttaataattc cataaaatat gctaacgcca gcattattgc catcaacgca aaaaaaatcg
    19981 aaaaccgaat aaaaatcagt gttcaggaca acggaattgg ttttgatttg gctactgtcg
    20041 aaaaagggaa tggtttgcaa aacatgcaaa agagaatcga ggaaatagga ggagaatttg
    20101 gtttagactc ttcaagcgaa ggaacttcta ttactgtttt attaaaaaag aactgattat
    20161 gataaaaata gccattgttg atgataattc gtttttgata aaaacggtac aagaaaaatt
    20221 gtcttttttt gaagatttct ctttgaaatt tactgctgta aatggtttgg atttaattga
    20281 aaaattagaa aaaaaccaca atgtcgattt gattttgatg gatatcgaaa tgccaaaaat
    20341 gaacggaatc gaagccactt gtttggtcaa acaaaaatat ccgcaaataa aaatcattat
    20401 gctcaccgtt tttgacaacg atgaaaatat ttttaacgcc atcaaagctg gtgccgatgg
    20461 ctatttgctt aaagaagtca accccaaaga tttgaatcaa ggaattatag aaacgatgaa
    20521 cggaggagca acaatgacgc cttcaatagc tatgaaaacc ctgaaattat tcagaaaccc
    20581 aatagctttc gaaaccattt cagacaaaga agaatacaat ttaacttccc gagagatcga
    20641 agttttagaa caattgagca aaggattaaa atacaatgct attgctgaaa acctgtttct
    20701 ctcggctggc accattcgca aacatgtcga aaatatttat gccaaactcc aagtgcataa
    20761 taaattagaa gctattcaaa aagctaagaa taataagttg atttgaaaaa taagccgttt
    20821 tactaattgt gtccggaaat tttatatttc acttttgttg aaaaataaat gaaatggaaa
    20881 ttttagtcta tttaggtttg attttagggt tgttatattt aatttttaaa tctagaaaga
    20941 ttagaaaaaa gtataaaata aagtcaaaaa aaatcaaagc tgaaatcaaa gcgctaaaat
    21001 taaaaagagc tttgattgaa gaaaggagtg atgcaaatca tttaataaca atcgattttt
    21061 ataaaatgct agaacaagtt tcggatttgc ataagttgat tattactaaa tatcttaaat
    21121 aaaatttatt tatttttgat gttatacgaa tttatcctgt aaattttatg aataaaaaaa
    21181 tacttttaat cttgttcgtt ttttctgctt ttttgagtgg agctcaaagt aacaaggagt
    21241 ttttagaaaa agcaatagtt tcaaaagatt cctctgattt ttattttcaa aaagctaaaa
    21301 atcaaataaa aagcaaagac gacgaagcgg cattttttta ttataaaaac gctttatgta
    21361 ccgaattaga gattcaagat agcgctattt attatggaaa aatagcttta aaaaaatttg
    21421 aaaactcaaa gaattataat tctattttga atctttatta taggattgcc aatgtttata
    21481 ataacaaagg acaatacgaa aaatcgatag attataatat ttctggattg aaaatagccg
    21541 aaaaaacggg gcttaaatat tgggaaggaa ccttctgtgt gaatctgtct tatgattacc
    21601 acgatttcga aaattattct aaaggaattg catttggcaa aagagctata caaattttca
    21661 caaccttaaa aaatgctaaa cccaaagaca ttaggtcggc atttaatgca actgcaatca
    21721 attatgatga ttggaataag cctgaaatgg ctctttatta ccacaagcaa aatctaattt
    21781 atctgaaagg aaaagacacc ttattattga atcataccta caataatatt gggaatactt
    21841 tgttaaaaca gaaaaaattt gaagaggcca aaaagtggat tttacgatcg gttgcgattc
    21901 ttgattacaa agtaaaacac aatgatatgg tatatataga ttataattat gcgacctgtt
    21961 atactaattt agcaacaatt gcatccgaat taaaccagta tcaagaggca caaaactatt
    22021 ttgatttggc tttaaaatat tccaaaaaat cacaggttgc cgaaaaaatt agagactatt
    22081 tttatcaaaa ggcattattc aataaaaagt acaataacct tgaggctacc atcaaagagc
    22141 aagaagatta tattgccttt cgagattcgg tttatacttc tgaaaggaat aaaacagcgg
    22201 ccgaattaga agcaaaatac caaaccgaga agaaagaaaa tcagatacta caacaacaag
    22261 ccgaatccag gaaaaaaaat atttggttgc tttttgcaac aagcatcttg ctaattgtag
    22321 ggatcttgtt tagaaatttt agaattaaat ccaaattaca aaaagaacaa ttggaattgg
    22381 aaaacaaatt attggaagaa caatccaact ataaaatgca agaacaacgc ttggaaattt
    22441 ctagagaatt gcacgataat gtggggtcgc aacttacatt catcatttcc attttagata
    22501 atttgaagag ttcgccagtt caatttgagc aagcgattga caaaaaaata gacaccttgt
    22561 ccaactttgc cggtaaatcc attgccgagt tgcgagacac aatttgggta ctgaattcca
    22621 aaaaattgag cttggccgaa ttgaaatccc gaatgttgaa tttcatcaaa gatgcaggcg
    22681 aatcagttga tggtactcaa tttcattttg attttgaagt caagaatgat gttcaattgt
    22741 cgtcaaaaca agctattaat acttaccgaa tattgcagga aatcgtcaat aatgccatca
    22801 aacacgccaa tgcaacggct gtttctgtcg caattaacca aaatcaaaag caattggaaa
    22861 ttcaaatttc agacaatgga attgggtttg attacgaagc aaaaaagaaa aaatcatttg
    22921 gtttgaccaa tattcaaaat agaattcagg aaataaaagg ggatatgaag gtgcagtcca
    22981 gtaacaaaaa cggaaccatt tacaccatta atatacagtt gtaacgatga taaaaattct
    23041 aatagtcgaa gataatttct ttttgcaaaa agcattggaa gaaaaactgt ctaatttttc
    23101 agatatcatc ataaaagaca tagcccaaaa cggagaagaa gccttggcga tattagaaaa
    23161 aaatcacgtg gtcgatttga ttctgatgga tatcgaaatg ccaaaaatga atggaattaa
    23221 agccacggaa attattaaat cgaaatatcc acaaatcaaa attgtgatca ttaccgtttt
    23281 tgataatgac gaaactattt ttaatgcgat aaaagccggt gctgatggat atttactcaa
    23341 agaaaccaaa gccgaaaaaa tatacgaagc cattggcgaa acactgtcgg gtggtgcagc
    23401 aatgtcgcca tccattgcaa tgaaaacctt gaagttttta aaaaatcctg ttgtttttga
    23461 agacttggaa acaccagaac aaatagcact ttctgaccgc gaaatagaag ttttggaaca
    23521 attgagccaa ggattgaaaa atcaagctat agccgataat ttgtttattt cattttcgac
    23581 tgtaaaaaaa catatcgaaa atatttacaa aaaacttcag gcacacaata gaattgaatt
    23641 gattcaaaaa gctaaacaaa acaagttgtt gtaaatatta gccgaacggc taattgattg
    23701 cttttgtagg aatcataatc tttgtatgga accaaaaatc aaagattatg aaaaaaatta
    23761 ccctattcat tttattgttt tcagcgatag taaatgctca aaatccgctt gtagtcaaag
    23821 acgtaacatc atcattaaaa cccacgggta attctgacaa agcagatttg aattatgcca
    23881 aatggaacgg taaactattt tacgctggta gtggcagtac tatgctttgc gttaccgatg
    23941 gaacaaccgc tggaacaatt ggactttctg ctttaggagg aactactaat atttcagcaa
    24001 taattcccgc tcaagatttt gtgtatgtaa ttaccagcga tatcactttt tcgccctcaa
    24061 cagcctcaac agacaaaatc tggaaaactg atggaacggt agcgggaaca tctttggttt
    24121 atgcttttga aacagctaca ggattaacca atgtgggagt ttattacagc gttccagcac
    24181 acagaaaaaa ctattctgtc gatggaaaca aactttattt ttcggctttt gatgcaacca
    24241 acgggaaaga actttggaaa accgacggaa ctgccgctgg aaccatattg gttaaagata
    24301 taaaacccgg aacaggaagt agccaatcat cgggcttttg taaaattcca agtactactt
    24361 tatttattgc tcaaagtgta ggtctagaaa gcaaattgtg gaaaaccgat ggaactactt
    24421 cgggtaccga acaaattcct gtggcagaac cgttttatat cgtcaatcaa gatatggcaa
    24481 gattggggaa taaagttatt ttctttgccc acaacacggt cgatggctat gaaccttatg
    24541 tgagtgacgg tacggcagca ggaactttta tgctgaaaaa tatcaatcca acgggtaact
    24601 cattaacaac acaagctatg ggtttacacc taaaaatgac cgacaggtat tgctttttta
    24661 ttgccaataa tggaactaca aacgctttgt ggagaaccga tggaacagtg gacggtacaa
    24721 tcgaagtgat tcctaatgtt accaacggca ttagtgatgc gggctattct acaactgatg
    24781 ccgaaaactt atggtttgta aattacaatg gcacaaatgc ggttcagcaa ttgtataaat
    24841 ccaatggaac ggttgcagga agttctcaag tttattcaac attaaatttt gctcaaaacc
    24901 tgactaccta taaaggcgct ttgtggttta gagccgccaa tagcggaaca ccagctaatg
    24961 ccgaagtgtg gagaagtgat ggattatcag taaacactgc attagcttta gatatagaac
    25021 ctaccattgt ttctggcatt cctatgagta gtaatcccaa tgcgtttttt gaattaaatg
    25081 ataaattgta cttttttgga aaaggcaata acaacaattc gcattatttg tatcaataca
    25141 ctggtgattt tacttttaac ggaagtataa gtaacgattg gaacaataag agcaattgga
    25201 atagtttgtt gaaacctggt atgacagatg tggtaacaat tccagccgga caaaatattg
    25261 aagtaaccgc aaacgcttat gctaaaaatg tggtcatcaa tggcaatcta aatttagttt
    25321 ccggaaattt agacttctct gggatgataa cattagccaa ttcggcaaaa attactttga
    25381 acaacaatat gctaaaccta aaaggggcag tatcgtcatt gacaggaaca aataccagtt
    25441 atgtgataac caatggaaca ggaaccataa acgttgaaaa tctggatgcg actagaggaa
    25501 cgatttcttt gcctattgga accgctacaa attacaatcc cgtttcactt tccaattcag
    25561 gaactagcga cacattttct gcccgagttt ctgatgggat ttcaggtgca acaaacggag
    25621 ccgtcaatgc tacttgggat atagctgaag cggttgttgg cggaagcaat gtcaatttga
    25681 gtttgggctg gaatcaagct caagaaaacg gcacttttga cagaaatgct gcaaaaatcg
    25741 ggcattttac agcaggaaac tggatgcagg aaacttctgg aaccgttttg ggaagcaatc
    25801 cttacacgct ttcgggaaca ggaattagca gtttttcgcc cttttcggta atgaatttct
    25861 cggctctttc cacatcggaa tttggtattg gcaaagtggc cgtttgtcca aatccttcta
    25921 atggcgattt tagtattgaa atcacgaacg aaatgatggg ctcaaaagcg aatgtgtatt
    25981 ctattcttgg tcaaaaaata aaaacctttg ttctgaattc taacaaaaca aacagcaatt
    26041 taacctcggg aatttatatc atcgaaattg aaaataacgg taaaaaaacc atcaaaaaaa
    26101 taatcgtaaa ttaagaagat atgaaaaaga aacttgtttt tagtgcaatc ttattttgcg
    26161 tgataaccac atctcaaggc cagtttttaa aaaaaatcac tggcaaaaaa gaaactgaca
    26221 ccaccaaagt ggaaaagaaa gtaggaggtg gtctttttca gaatattgtg gcaaaagtgg
    26281 caaaagttgc tggaaatgcg gctggaggaa tggccggaat ggtcagtaca gttgataatt
    26341 tagaaaatgt cgatatttta gcttcgatag gaaccaatat ttattctaaa aaattgggtt
    26401 tggttgtcaa tgatttttta ggagacgatt ggattgataa tggcgatttt acgatgataa
    26461 tgttgtcgtc aaagaatagt tttcagctca ataaatatgc gggaagcata aaagtcaaca
    26521 acaaagagtt gaagcactct tcctacggag ttcataccgt ttgtgagaat ccaaattcag
    26581 gaaataaaaa aatctctttt gagaaaaatg gtattgtaga aggttctttt gaagttccag
    26641 cactcaaaaa caatataaaa ttattgacaa taaacgggca aaaagacaat gtaaatatcg
    26701 atttgactaa agatgttgtc ttggaatttt caaactattc gactacaaaa gaatcgcttt
    26761 tgcgcataga tattattgct tcggtcatag gaattcgatc cttgtatttg gtaacttatg
    26821 tcaaacctgc agcaaaagtg gtcatccctt ttcaagcttt cagaaatatt gaaacaatga
    26881 gcaaaggact tgggttttct aattgttacc tttcggtaag cgatcaattg gcagtgaaaa
    26941 ccataaataa tactggattt tttaagtctc caatcgatgc tcttacgggg tcgaatgatg
    27001 ggatgtggat caatgtaacc caaaaaccag agcagtttta tggaatatcc atcgaaaaca
    27061 aaaccaaaac aacagaagtg acggcccaaa agaaaaatgc agcctacagt atgcctttga
    27121 gcaaagccca aaaaattgct gtcgcatcgc ttagtattga aggaaccacc tattccgaat
    27181 cgtcaaaagt caatgctttc cagcaaacag aaaccacaaa aacgatcgaa tttccccaaa
    27241 ttcccgatgc ttggcaagag gccacattgg tagaaatgca caaaaatatt actgcaacca
    27301 ccggtcaaat cctcggagga acaatcttgc ctgttgaaat tgttacacaa gccccatctt
    27361 ataatgaagt ccaaaacttt tttagagaag aaacgaatac gagttcaaat ttcttgagag
    27421 catacaaagg tttgaatcct attaagccat taagcacatc cagtttaaaa atgtatggcg
    27481 aaaatgcact tttatcagaa acaaaatcgg atgctttgtt gaaaataaaa atggttttag
    27541 aattgtcttg ggacagcaag cctttgatga caccattcct gtatgttaca atggatggcg
    27601 cttcaaacgg agcattccga tcggcatcag gcaacacaaa ttattttaca ataaaaataa
    27661 aaggggagcc ttatgaaatc aaaaaaggga aagtcataac tccagacgaa tacgtaaaaa
    27721 taacccaagt agctcttttg aatcaaattt ataaagaaac tttaagcagt ttgaaacaaa
    27781 aagagttaga gaaaggagat tatgaagtaa tctggaatct tcaaaaataa ttagctaagc
    27841 cgttttttat caaacggctt ttttattact tttataaaaa aaaatataaa tgcaaagaag
    27901 aatcttaata ggtgcgggaa ttattgtggc aatcattttg cttttcaaat tctgtgattt
    27961 caaaaaagaa gatgattcca ccgtagatta caataccaat ctcattcaac agcaaattgt
    28021 taacgtagga aagctggtcg ttaccgaagg gcattttgcc gaagtgatta cgtataaaga
    28081 ccaagataaa tacctgatgg atatggtttc tttcgaaaag aaagccatag tcattgtcaa
    28141 tgccgatgtg actgttgctt atgatttgca tcaaatgaaa tacgatatcg acgaaaaaaa
    28201 taaaaccatt accatcaaaa atattccaaa agaagaaatc aaaatcagtc cggacattaa
    28261 atattatgat gtgcaacaaa gccaaatgaa tccttttacg ggcgatgatt ataacaaaat
    28321 aaacaaaata gttcgagcca atttggcaaa gaaaattgaa aaatcatctt tgaaaaccaa
    28381 cgcccaaaac cgattgattt ctgaactttc gaaaatgcta attcttacca atacaatggg
    28441 ttggaaattg caatacgaag gaaaagtgat cgaaaaagaa gctgatttta atcaaaaaat
    28501 taagggttaa tttaaatctt caaacaaatc atcgtttact agattaggta gcgtaacttt
    28561 cagtaaaggt tgtgtttcca tggctctttt tatggcaaaa attgctcctt catttcttgc
    28621 ccaacttctt ctggaaattc cgttgttgac atcccaaaaa agcatagatt caatgcgctt
    28681 tgaagcttct cttgagccat caagaaccat cccgaatccg ccatttatga cttcgcccca
    28741 gccaacgccc cctccattat ggatagaaac ccaagtagca cctcgaaaac tatcaccaat
    28801 cacgttctga atcgccatat cggcagtaaa acgagaacca tcataaatat ttgaagtttc
    28861 tctataagga gaatctgtgc ccgaaacatc gtgatgatca cgtcctaaaa cgacagttcc
    28921 aatttcgcct ttggcaatag cttggttaaa agcttcggca attttcattc taccttcggc
    28981 atcagcatat aaaattcggg cttgcgaacc taccaccaat ttattttctt gagccccttt
    29041 aatccactga atattatcgg ccatttgctg ttgaattcca gcaggagaat gttgcatcat
    29101 ttcttctaaa acttcgcaag caattctgtc tgtcttttct aaatcttctg gctttcctga
    29161 ggcacagacc catcgaaacg gaccaaaacc atagtcaaaa cacatcggtc ccattatgtc
    29221 ctgcacataa ctcggatatt tgaaatcgat tccattttcg gccataatag ctgctcctgc
    29281 tcgagaagct tctaatagaa aagcatttcc ataatcaaaa aaataagttc cttttgccgt
    29341 atgtttatta atggcattag tatgtcgccg caaactttct tgtacttttt ctttgaataa
    29401 atcaggatta ttggccatca tttcattgga ttcttcaaat gaaattccca cgggataata
    29461 ccctcctgcc caaggattgt gcaacgaagt ttgatcggaa cccaaatcga taaaaatatt
    29521 ttcttggtca aatttttccc aaacttcgac cacatttcct aaataggcga tggaaacaat
    29581 ttctttattg gctttggcca aattgactct ttttaccaat tcatcaagat tttcaataat
    29641 ttcgttaatc caaccttgac tgtgtcgaat gt
//
LOCUS       DAOSNN010000004        29140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_1615_length_29140_cov_5.363452, whole genome shotgun sequence.
ACCESSION   DAOSNN010000004 DAOSNN010000000
VERSION     DAOSNN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 29140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29140
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1615_length_29140_cov_5.363452"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="PLQ70_00415"
     CDS             <1..1716
                     /locus_tag="PLQ70_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE33108.1"
                     /translation="PIFTLSHYWKFNTNTRLNTNVSYQFGQIGNSRIDYQKADNPDPN
                     YYKKLPSYYTTLYTYSNDVATYTPDYAAAEEAKANFLAQPQMDWTAMYRMNNENITNG
                     SRYVLYEDRTDDKTLVANTILSSQLADNIVMNAGGSFTKSKSSNFKNLIDLLGGTHFN
                     DINTFGSTEDEQQSDLNNPARTVVVGDKYGYNYIIDATKIDAFTQFKFTYDKVDFYLG
                     QSFTRSSYQREGLYKNGYYPDNSLGKSEKLNFDNFGFKGGMTYKLTGQHYFDFNGIYM
                     TKAPNTKDVFPNARLSNDITDNIKNETIKSVDASYIIRMPSLKARFTAYFNETENTTD
                     IAFYYADNISVGNGEFVSEVVSGQNKRNKGIEAGLEYQITSTIKATAIAAFGEYTYTN
                     NPNLAIHADGVTTDIFNGKATMEGYKQAGMPQQAYSAGLEYRDPKFWWIGANVNYLSN
                     NYLDVAPLLRTSQFITNSDEANFPYDAATAASFLAQEKFDAFTLVNLVGGKTWRIKEK
                     TLGLFANVNNVFDIVYKTGGFEQARNASYSELYKDHQGPTRSFGPKYFYGYGRTFMVN
                     LYLNF"
     gene            1729..3150
                     /locus_tag="PLQ70_00420"
     CDS             1729..3150
                     /locus_tag="PLQ70_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HQE33109.1"
                     /translation="MKNSIKNSCIILATLGLFSSCVNDTYDTPKFDCVNPGLTKTKEV
                     NDLYTSAPANGTTLIYPAAAKDPVTGKEIFDYIEAYVISSDEGGNFYKSMYFQPTDGS
                     KGFNLSIDEVNLYGKNFQPGKKVFLKLNGLAYANPTSFGRGLIFGAPPTEQFAVDRLA
                     TLVYPKHLIASCDAVSEDAIVKKITLAQALNGAAYLNTLVEIDDVQFTDEAAGGTYDS
                     NRNDDFDSSIFVTNGTSNLTIRTSRFANFAGYKVPTGKGKIRGVLTRYNSTYQIIMRT
                     ERDANMPNPRVDYTPAIIGANSNVFPSTVNETFESYTSSPSQTNFPNYINEAAVGSRY
                     WGVLTFSSNKYIQMTSFGSGGANNTYLFVPVNMTAASTFSFKSNMGFWNGAVLNVYYV
                     LATNYTPGGKVDTSKMVNITSNFTIPTTPTSGYGTTFTASGVYAIPATVTGNGYFVFE
                     YAGNANAFPAVTTTLQLDNISVN"
     gene            3227..5191
                     /locus_tag="PLQ70_00425"
     CDS             3227..5191
                     /locus_tag="PLQ70_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HQE33110.1"
                     /translation="MKKFYTLLLLTFSYITSGQVVINELDSDTPSTDDKEFIELKSTT
                     PNFPLDGYVLVFFNGNATSTTANKSYYTIDLDGLTTDANGIVLIGNSLVSPSPDKIFP
                     DNIIQNGADGVGLYLGNSSDFPDDTLATTTNLIDALMYDTKDADATALMALLGVSVQY
                     DEGQNSLQTTQSIQRKNDGTYETKNPTPGANNDGSGIIFNGISITANTVNKNEGDSFP
                     ITFTIQTNVTSDLTFTFTLNNSSFTSADFSGNTTVLIPSGSNTFTTTITLIDDVLNEG
                     DEVLKIKIGAIPVQYKKLNDNIEIRVVDNDFTVAPFGTPLNPTYGIVSSTAPAGYYAS
                     LEGLSGAALKQAIQDIIANPAVVHAHNYGDIIDILKTADQNPLNSNEVWLMYVEQGRS
                     KLEFQDTGINTGKWNREHIYPQSRGGFTDGTESIPDGINLWLPTNADDILAGHADAHH
                     LRAEDGAENSLRNNNDYGLTGYNGPSGTKGSWKGDVARSVFYMAVRYNALSIVNGDIA
                     DTTVGQLGDLASLLTWNTLDPADDFEMNRNNYIYTWQVNRNPFIDYPDLANYIWGAKA
                     GQTWHFNLSNPDFVNLKVTLYPNPAQKSITISGLNEEANIEIYNSLGAKILEQKIYGE
                     TKLAIDFQTGVYYAKIHSEDKTVVKKLLIE"
     gene            5301..6197
                     /locus_tag="PLQ70_00430"
     CDS             5301..6197
                     /locus_tag="PLQ70_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HQE33111.1"
                     /translation="MITPAYLQKGDTVAIVSTARKNIDDNLKPSIDLLHSWGLEVVIG
                     STIGLDYNQLAGTDEQRAADFQQQMDNPNIKAIWCVRGGYGTVRMIDLLDFTKFKQHP
                     KWIVGFSDVTVLHNHLNTMGFKSIHGTMPVSIATTAPESKASLKSALFGEPISYKINP
                     FPMNRFGKATGELVGGNLSILNSLFGSKSAIDCTDKILFIEDLDEYLYHIDRMMMNLK
                     RNGCLESIKGIIVGGMTKMKDNDIPWGKNANEIVQDITQKYNIPILYNFPAGHVHDNR
                     ALIMGNTVSLEVNENGGTVVFE"
     gene            6319..6678
                     /locus_tag="PLQ70_00435"
     CDS             6319..6678
                     /locus_tag="PLQ70_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQE33112.1"
                     /translation="MAQHNELGKLGEELAVEFLQKEGYEILETNWTFQKAEIDIIAKN
                     ENTLAIVEVKTRSSLEFGLPQDFVKPKKIQLLVKAVNEYVISKNIDIDVRFDIIAIHK
                     EDKSFVIEHLKDAFYHF"
     gene            6786..7442
                     /locus_tag="PLQ70_00440"
     CDS             6786..7442
                     /locus_tag="PLQ70_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQE33113.1"
                     /translation="MKTVSSIVENYIKTKPFLLNALSLGIINLTSLSRNIMVELESEF
                     GKEVKQGAVVMALKRLTEELDFRLNHKINKVIKNIGEITVRSSLTDYTFAASDTVLNK
                     QADLITDINTFSDIFYTSSRGVNETNIVVSNTVNHLVDKHFANERLIQKLENLASITV
                     KLPKENIVVPGIYYFIFQRLAWEGIIINEVISTSNEFTILVSEDEVDIAFKVIKDLKN
                     "
     gene            7565..9670
                     /locus_tag="PLQ70_00445"
     CDS             7565..9670
                     /locus_tag="PLQ70_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HQE33114.1"
                     /translation="MLAPAYQKLKDNLSKSIDPKRILDNPLQTLAYGTDASFYRLIPK
                     IVILAHNETEIIEIIKQAKVLNIALTFRAAGTSLSGQAISDSVLVVATHGWKNFELLE
                     NNQKIKLEPGIVGARANTFLAPHGLKIGPDPASIGAAMIGGIVANNASGMCCGTAQNS
                     YQTIADIRIVLNDGTILDTANQDSVAAFKNNQKGLIQEIESLRDTIKNDETLYHFIKN
                     KFKIKNTTGYSINALVDYQDPIEIIKHLMVGSEGTLAFISNVTFKTVIDEKYKSCSLI
                     IFNTIQDACNATILLKSSPVAAVELLDRESIRSVEFDPDAPTYFKTLPESACALLVEC
                     RDNDLEVIHQKQEQIRLQIQSIPTYTDYEFTRDPKQYYFNWKARKGLLPTVGGLRKNG
                     TSVIIEDVAFPLPQLADACLALKDLFKKYEYHDAVLFGHALEGNLHFVFSQDFSNQTE
                     VDRYEKLMAELADLVVNQFNGSLKAEHGTGRNMAPFVEKEWGTAAYEIMKHIKNIFDP
                     HNKINPDVLINPDPKAHLKNLKPMPESHHIVDKCMECGFCEPHCVSEGLTLSPRQRIV
                     IAREISRLEETNEDPTRLAEIQKDVTYQLDETCATDGLCALACPVNIDTGKFVKTWRA
                     NELKSGNKKVAAYIGSHMAGTTAILRVGLKMVSFFHSIFGTKIMTALSNGSHFISFGK
                     IPKWIPEMPKGAHKINTKN"
     gene            9929..13999
                     /locus_tag="PLQ70_00450"
     CDS             9929..13999
                     /locus_tag="PLQ70_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQE33115.1"
                     /translation="MKKIVLYFVFVFSLAGFSQEYPVKFLDISDGLSNNSITAIHQDS
                     DGFMWFGTYDGLNRYDGYNFKVYRNRINDKNSLSFNTIYCIEGDSRKNIWVGGANGAC
                     VFDKKKSIFHPIEYLSSTKTQTVKDVIHQIRSVSKDLVLVASQNFGLLVYKNGSFQGK
                     SIALEQSNNPIEKYNYNAAVLENDSKNSSCWVFVRNIGICKYRYHSNKLEVVYPLSME
                     VKCMKLSVDNTLWLGTDEGIFSFNTKTFSLSANYLINNCTVTDIVIDKKKELWLTTDG
                     YGIYKIIDKNKKAFPFQFANGQQYIKSNSIWSLYEDIAGNKWIGTLRGGISMIGNSPK
                     YFKNFKYNVKVNEPAENFILSFCEDEKKNLWIGTDGAGLKYWDRKSNSYTIYGNSNNS
                     TYKISSNFITSIIRDTDNQIWVSTWAGGINRINPVTHSVTRFSCYNSFTKQIEKNVWF
                     VYKDSDDDIWASATNEGSLYLFDRAKNTFVLFNKSIDNLQCLIETSDGKFWGCNYTSL
                     LAIDKKTKKYRKFPIGNSIRSLHEDKDKNLWVGTQEGGLLLFDRKTNKFNRLTTDDGL
                     PSNTILRILEDKAGNLWMSTYNGICRFDKKNRTFRNFSVTDGLQSNQFSFNAGLKLSS
                     GEFLFGGINGFNSFFPEDIKEFNQKYNLLLSDFYVNNQSIEESKNELITNWDSGKIKE
                     VNLPYDQTTLSIEFVALDYNNADKINYAYYLEGWDNRWNYVGQARKANYARLTEGTYV
                     FKVKTTNFRGGWNKEENLVTIIVSPPWYRSWWAYTFYLLAGAGLIYGYVRYNKNKEKL
                     KYKVKIAELEGAKEKEIAEKQSSMFTYISHEFRTPLSLIINPLKKAIKTGNLENSSSV
                     TDLVIAHRNARRLLSLVDQLLLFRKAENDADELKMSVINVNNLCYEVFQCFVNQAKDK
                     KIDYTFQIPENNIEIIGDYEKIEISLFNLLSNAFKYTPDGREISLVLSETETNVSIEI
                     IDSGNGIAKNDLEVIFDKFRQVNSKTPVGGSSGFGIGLFIVRYFIEKHKGTVSCTSEI
                     GKGSVFKLTFLKGEDHFENLQISTVTPKMSPLVEELMADDTEAGIEVSVNAISENELH
                     KAILTDKRTLLIIEDNTDIRNYLIRLFSEVYIIYSAENGEEGLKLTKKHMPDLVISDI
                     AMDVMDGFELCRKIKENSSLSHIPVILLTASKNPETHLQGISDGADDYITKPFDDDLL
                     MARVESLLKNRSNLRAYFLDSITLKENTQKVPAEYQEMLKKCIAIIEANIHERDFTIK
                     NFATEMGMSHRTLYTKIKIISGQTLNAFIRSIRIRRAAMLMLTENMNIAQASAEVGFE
                     DPKYFRQQFVKLFGITPSEYIKKYKKSFDSDLNIIN"
     gene            14682..16079
                     /locus_tag="PLQ70_00455"
     CDS             14682..16079
                     /locus_tag="PLQ70_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33116.1"
                     /translation="MKKITFLFFLTFFTILYTGCQDNGPDYPSSDNPTVVAATTEIYG
                     APNKKFEIKAALADDLGLKTVRIQIPELSLDKVITFETDPLLTTYNLSYFFEIPADRG
                     TSEAFKIKLTVTDVSNNSVDQEIKLRLDGEFDAPAVTMITPKEGAVILLSTSNQMPVN
                     FTVNDNSGIDFVELKCDALGINELVQLQGAPQSYTFNKTFTLPVTAANYVLTINTRDK
                     FAIPNSGTLKINVVATNEYPALYLCDQPKGTNLTTDAVGVPMYFHEKVGQDFTFKYYA
                     DSDNKEVYFLGQESDFAPHCFGLNSSGKLADDVASSPIVLPTKGYYLIKVNPNTLLYT
                     VTKYTPTSKVWSNIANGLWHDDAALRRPFVSVCGGGIQGANWDTWNAWTQTSLHLANN
                     SNNPYQLVGEYTLTGTMECTFTGQWWDPSWRLIKNGIATMSPGGNGNAKYPAAPGVYK
                     VIVDTELERAFITKK"
     gene            16128..17090
                     /locus_tag="PLQ70_00460"
     CDS             16128..17090
                     /locus_tag="PLQ70_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQE33117.1"
                     /translation="MKFKHIWLFIAVLCSATTFGQITVKGTVKDNKALPIPGVNVSVK
                     GTTTSASTDLDGKYAIIVPNKEAQLEFSFIGFTNKIVKVGDKTLVDVMLEESNQALDE
                     VVVVGYATVKKKDVTSSIASVKGKELQTMTVGNATESLQGKVAGVQITGAGGPGAQPR
                     VLIRGISTVNLSTDPLYVVDGIPMGTSINFLSNNEIESMDVLKDASASAIYGSRASNG
                     VILITTKKGKVGKPKFNVDLSNGLQIMNNPYNMADAESYANILNKAYTNSGYSEYLPN
                     AEQYRGKTTDWWGAGINKTSQVSNASIGVSGGSDKNTYAIFKLL"
     gene            17068..19137
                     /locus_tag="PLQ70_00465"
     CDS             17068..19137
                     /locus_tag="PLQ70_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HQE33118.1"
                     /translation="MLSLNYYNSDSFYNIGGWERVTARIANDFKFSDKISMGITLNPR
                     YETWGSPGNWADFDKIDPITPIYKPADQLNGTENEYSIYARSPSYVWNPVASVARYDD
                     YTNLYNLNTNAYFQYEPIKGLVFRTQGSIEIGDKTQSIFRPDFEIDAAHEKAEINSVE
                     RKNTTNHDWTWQTTATYSKTFAQKHNASLMVGATMEEYNGNDVWGYGEGVPNNSDLMR
                     EVNAATKNRNSGGNSWSSSLQSYISRLSYNYDGKYYLTGTFRRDGSSKFMANNKWANF
                     PSASASWRISNEGFMENTKAVVNDLRLRAGWGKVGNQSLPGSVYQSNIGQGYYVIGNE
                     VVDTSYPSSMANKDIKWETVEDINFGIDFGLWKNKLSGSIEYYEKTTEDMLFKKQFPT
                     YSGFPGYATIWTNVGSMKSNGIDVLLSYKDKKGDFSYGADFTFTTSNVKMVSLSADGE
                     KLYGSSNRTLTIEGDEPGYYYGYIADGLFQNQTELNSHTDEHGNKLQPYAQAGDVRFK
                     DLNNDGKIDDKDRTKLGSPWADFTMGLNLSVAYKNFDLIANFYASIGNELINQNISDL
                     YNGASLTNKVAGLDQMAWNGEGTSNYVPRLSKNDNNENFTKFSSLYVEDGSFLRMKNI
                     QLGYTFLNQFGLDKLRISISGQNLWTLTNYTGVDPEVGGGVLGSGFGGWNYPVQPTVL
                     MGINVGF"
     gene            19149..20756
                     /locus_tag="PLQ70_00470"
     CDS             19149..20756
                     /locus_tag="PLQ70_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQE33119.1"
                     /translation="MKKIIISIIAFSLLAVSCENFIEKEERGTQTLENYFQTAQECEN
                     YVNELTQRMLLSKDWYTLLAPRLINETASDDAWMGNTGQDNSAFRPGAQYVITPENMG
                     ALNSLYTAHYYTIQSANIGLEKMANTPISESQRNQYMGESLFVRAYCYYELVNNFGAV
                     PLYTKSLGTNDLKLERSPVADVYAQIEKDLKESATKLENIPVDRNGRVNKWAAYALLA
                     RVSLFQEKWTEAKQYSNKVITEGPFALEANFLNIWNVNNHNGVESILEAQSSSVQDKS
                     LGSMLPTFSGARGEDKKNFPSNDAKDVIDGWGWCMPTSDLENAYLSENDVVRRRSTIT
                     KWGEAAYGDEVLNPTHKFSLNDNKSGRICRKYYIPIATRRALDKKDGHLPLNVPLIRL
                     AEMYLTRAEANYQLKDEPGALNDINVIRVRAKLDPKVGLLGPNLLRQIYKERRLELAF
                     EGLRLFDIRREKDPSTSKPVIESLLGPNGTFVKYNLSSTDPFETTNLKESQDKGINFN
                     PAKNLLWPIPQLERDLSGGLITQNPGY"
     gene            20758..22401
                     /locus_tag="PLQ70_00475"
     CDS             20758..22401
                     /locus_tag="PLQ70_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQE33120.1"
                     /translation="MINIKNYKVSLLFAGLLLINSSLTSCSSSDNTDNTQPPTTTTRD
                     LNVNLDLKLQTMESFGASDAWQCNFIGKNWPLDKKNQIADLLFSKDVDASGNPKGIGL
                     SLWRFNLGAGSSEQGNASDIGDEWRRTECFTTDGISYDMNKQAGQVWFMKAAKARGVE
                     KLLAFTNSAPVYLTNNGKAYASIKEFYNLKDGKMPELADFWVNSIDKLKTVHGLTIDY
                     VSPFNEPQYEWNGTNQEGSPATNANIYSFVNALSPKLLAKGLNSQIVVGEGGAFEPLY
                     KTVSGTESRSNQIDYFFGTNSTKNIGGLSNVKKTISAHSYWQAWPLSSLVSSRQSAAS
                     KIQTVPGLNLWASEYCILESPGTAELPGGAGSGRDLGMQTALWVARIVSSDIAIGGVS
                     SWQWWTAVSRGDYKDGLIHVDDGASNGAGGADYCKNDGYVRESKILWALGNFSFFVKP
                     GMARVQIPNIDNSTATTDVMLTAYKDETNKKLVIVAVNFSKTARTYKLNLSGGTLTNN
                     KLTPYTTSDALSLKKGTDVDPANFEIGARSVVTYVGTYK"
     gene            22622..24205
                     /locus_tag="PLQ70_00480"
     CDS             22622..24205
                     /locus_tag="PLQ70_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQE33121.1"
                     /translation="MKNTYITFLLLLSSLAFAQRTATINTDNVLQTMDGFGASDAWRC
                     QFVGKNWPDQKKNAIADLLFSKEVDAQGNPKGIGLSIWRFNLGAGSIEQGENSKVSDQ
                     WRRSECFLNADGTYDWSKQEGQRWFLQAAKKRGVEKYLIFTNSPPVHMTNNGLSFATQ
                     KNKLNLKEGTIPKFADFLVQSIQGLEKKEGMKFDYVSPINEPQWEWMPNNGDENSQEG
                     TPATNQEIYDLTKALSDKLKSQKMNTEIVIGEAGQINYLYENVNDENRDNQIDYFFGK
                     GKTNISKLPNVRNTISGHSYFTTWPIDRQVLSRKLIAANVKQKPGLKYWQSEYCILEN
                     PGETEIPGGSGGTRDLGMPTALFVARLIHNDVALANAASWQWWTSLTRADYKDGLIYL
                     DDGKSNGGTAPDYVKNDGEFHDSKLLWALGNYSLFVRPGMLRVDVPNQDELASANDVM
                     LTAYKDAANKKLIIVAVNCGTSDQNYKFNLSKGTLKNNELTPYITSESSNLKKAALQK
                     IDTIVIPAKSVVTFVGEFQ"
     gene            24293..25219
                     /locus_tag="PLQ70_00485"
                     /pseudo
     CDS             24293..25219
                     /locus_tag="PLQ70_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
     gene            25312..27735
                     /locus_tag="PLQ70_00490"
     CDS             25312..27735
                     /locus_tag="PLQ70_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQE33122.1"
                     /translation="MITKKILLPIFIFSCLSATSQISFGDSQKINNNWKFTLQDTPEA
                     SSETYKDTNWQSVNVPHDWSVKGRLSPTLASCAGFLPGGIGWYRKTLNIPQTKRGEKV
                     YLYFEGVYNRSEVFINGHSLGKRPNGYISFAYDATPFVKYGEDNVIAVKVDHSLSADS
                     RWYTGSGIYRNVWLVYSNPVHIAQWGVYTYPEVANGTGILNVEVALENGSTAKTNLTV
                     VNELLDNNGKVVAKSSQKVLVEANKDNKVATKINLKNPKLWDVEHPNLYQLKTTVLKD
                     GKPIDKTVTSTGFRNFTFDANKGFALNGKWMKMKGVCLHHDAGVLGSAVPRDVLKTRL
                     QTLKEIGVNAIRTSHNPQAPDFYALCDEIGLLVLNEAYDEWEFPKRKWLEGWNVGTPG
                     FQGSNDIFAAWGEKDLEDMVRRDRNHLSVFAWSIGNEVDYPNDPYSHPVLDGGKDTGF
                     GQAAYGGYKKDAPDAMRLGGIAKRLVAAVKKYDKSRPTTAGLAGVAMSNETEYPGVLD
                     ITGYNYTESKYQSDHKKYPNRVIFGSETRHEIEPWLAVKNNEHIFGQFLWTGIDYLGE
                     SNAWPSRGFYSGLVDLAGIIKPRGYFRQSLWSNKPMIYAGTYPVNNSKELSIDAWPLW
                     NYNNGQTIRVVCYTNAAKARLELNGKVVGEEKKYDEKTGVIYWDIPFSSGKLEAIGLD
                     NDGKVVSQYAITSSQQPVSLVINEKNITIDKEKEVAKVIVQVLDEKGLPVMLSDNELT
                     CVVSGSGTLLGLEAGNNEDMGDYTDNVQRVFHGHLVAYIQSTGSNEPITVKFTANWLK
                     SAEVTIQVK"
     gene            28110..>29140
                     /locus_tag="PLQ70_00495"
     CDS             28110..>29140
                     /locus_tag="PLQ70_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HQE33123.1"
                     /translation="MNLSKFSKIVFTILCFSAFNSVQSQAQKEFHSWASTPPMGWNSW
                     DCFGPTVTEAEVKANANYMAKYLKPYGWDYIVVDIRWYVGNDKAHGYNEKDPDYVLDQ
                     YGRFMPAINRFPSAAGGKGFKPLADYLHKKGLKFGIHIMRGIPVIAVKQNLPIKGTNF
                     TAKDIYSDKDQCKWLHDMYTVLPEKPGAQEYYNSLFELYASWGLDFVKIDDLSSPIYF
                     EGEIDMIRKAVDHTGRKIVLSTSPGETPIAHADHVQKNANMWRTVGDFWDSWQQLKEH
                     FEVFERWNKWRAYGAYPDGDMLPLGRIGIRAERGDPRMTAFTKDEQYTLMTLWSIFKS
                     PLMFGGNLP"
BASE COUNT        10007 a         5034 c         5500 g         8599 t
ORIGIN      
        1 cctattttta ctttaagtca ttattggaaa ttcaacacaa acacaagact taataccaat
       61 gtttcgtatc aatttggaca aataggaaat agccgtatcg attatcaaaa agccgataat
      121 ccagatccta attattacaa aaaattacca agttattata caacattata cacctacagt
      181 aatgatgtag ccacttacac tccagactat gctgctgccg aagaagcaaa agcaaatttc
      241 cttgcacaac cacaaatgga ctggacagca atgtatagaa tgaataatga aaacattaca
      301 aacggaagcc gttatgttct atatgaagat agaactgacg acaaaacttt ggttgccaac
      361 acgatacttt cttctcaact agccgataat attgtaatga atgccggagg tagttttaca
      421 aaatcgaaat catccaattt caaaaatcta atcgatcttt tgggaggaac acattttaat
      481 gacatcaaca cttttggttc aacagaagac gaacaacaat ccgatttaaa taatcctgca
      541 agaactgttg ttgtaggcga taaatatgga tacaattaca ttattgatgc cactaaaatt
      601 gatgctttta ctcaatttaa attcacctac gacaaagtag atttttatct tggacaatct
      661 tttacaagaa gcagttatca aagagaaggt ttgtacaaaa acggatatta tccagacaat
      721 tcattaggga aaagtgaaaa attaaacttt gacaatttcg gatttaaagg aggaatgaca
      781 tacaaattga ctggacaaca ttacttcgat ttcaacggaa tatatatgac aaaagcaccg
      841 aacactaaag atgtttttcc taatgctcgt ttaagcaacg atatcacaga caacatcaaa
      901 aacgaaacta taaaaagtgt tgacgctagt tacatcatca gaatgccaag ccttaaagct
      961 cgtttcacag cttattttaa cgaaacagaa aacacaacgg atatcgcttt ttattatgcc
     1021 gataatatta gcgtaggaaa tggagaattt gtttcggaag tagtttctgg tcaaaacaaa
     1081 agaaataaag gaattgaagc aggattagaa tatcaaatta cttctaccat aaaagctact
     1141 gctatagctg cttttggcga atatacttat acaaataacc ctaatttagc gattcacgct
     1201 gacggggtta ccacagatat tttcaacggt aaagcaacta tggaaggcta caaacaagcc
     1261 ggaatgcctc aacaagccta ctctgctggt ttagaatatc gcgatccaaa attctggtgg
     1321 attggagcaa acgtaaatta tttgtctaac aattatttag atgttgcacc attattgaga
     1381 acttcacagt ttataaccaa ctccgatgaa gccaatttcc cttatgatgc agcaactgcc
     1441 gctagttttt tagcacaaga aaaatttgat gccttcactt tagttaacct tgttggagga
     1501 aaaacttgga gaatcaaaga aaaaactttg ggattgtttg ccaacgtgaa taacgtattt
     1561 gatatagtat ataaaacagg tggattcgaa caagccagaa acgcttcgta cagcgagtta
     1621 tacaaagatc accaagggcc aactcgctct tttggtccaa aatatttcta cggatacgga
     1681 agaacattta tggttaattt atatttaaac ttttaagaat taagctttat gaaaaatagt
     1741 ataaaaaata gttgcatcat tttggcgacc ttggggttgt tctccagttg tgtgaatgat
     1801 acttacgata ctccaaaatt tgattgtgta aatcccggtt tgaccaaaac taaggaagta
     1861 aacgatttat acacaagtgc accagcaaat ggcacaacac taatttatcc tgctgctgca
     1921 aaagaccctg taactggaaa agaaattttt gattacatcg aagcttatgt gatttctagt
     1981 gacgaaggcg gaaatttcta caaaagcatg tattttcaac ctactgatgg ttcgaaagga
     2041 tttaaccttt ctatagatga agtgaatctt tatggcaaaa actttcaacc tggaaaaaaa
     2101 gtgtttctaa aacttaacgg attggcctat gcaaatccaa ccagttttgg tagaggctta
     2161 attttcggag cacctccaac agaacaattt gcagtagata gattggctac attagtttat
     2221 ccaaaacacc ttattgcttc ttgtgatgca gtaagcgaag atgctattgt caaaaaaatt
     2281 actttagcac aggccttaaa cggagcggct tatttgaata ctttagtcga aattgatgat
     2341 gttcaattta cggacgaagc tgctggcgga acctacgatt ccaatagaaa tgatgatttt
     2401 gattccagta tttttgtaac aaacggaaca agtaatttaa ccattagaac cagccgattt
     2461 gccaattttg cgggttataa agttccaaca ggtaaaggaa aaattagagg agttttgact
     2521 agatacaatt caacctacca aattattatg agaaccgaac gggatgcgaa tatgccaaac
     2581 cctagagtag attatacccc tgcaattatt ggtgcaaatt caaacgtttt cccatcaaca
     2641 gtgaatgaaa ctttcgaaag ttatacttct tcaccatctc aaactaattt tccaaattac
     2701 atcaatgaag ctgctgtagg ttcaagatat tggggagtat tgacatttag tagtaacaaa
     2761 tacatccaaa tgacttcgtt tggatctggt ggtgctaata acacttattt atttgttcca
     2821 gtaaatatga cagcagcaag tactttttca ttcaaatcga atatgggatt ttggaacgga
     2881 gctgttttaa atgtttatta tgttttagca acaaattata ctcctggagg aaaagttgac
     2941 acttcaaaaa tggtcaatat cacttcaaac tttaccattc ctactactcc aacatcgggt
     3001 tatggaacca cctttacagc tagtggcgtt tatgccattc cagcaacggt aacaggaaac
     3061 ggttattttg tgtttgaata tgctggaaat gcaaacgctt tcccagcagt aacaaccact
     3121 ttgcaattgg ataatatttc agtaaattaa tcttttattg tgaaaaaata aatctaaaag
     3181 gcttccgtga tatggaagcc ttttttattc tttaaatttc attccaatga aaaaatttta
     3241 taccctactc cttttaactt ttagttatat aacttctggt caggttgtta tcaacgaatt
     3301 agattctgat acaccaagta cggatgacaa agaatttatc gaattaaaat cgaccacgcc
     3361 caactttccg ttagacggtt atgtattggt tttcttcaat ggaaatgcca cttccaccac
     3421 cgccaacaaa agttattaca ccatagattt agacggattg accaccgatg ccaacggaat
     3481 tgttctcatt ggaaattcac ttgtttctcc atctccagac aaaatatttc cagacaatat
     3541 cattcaaaat ggtgccgatg gcgttggatt atacctagga aactcctccg attttcccga
     3601 cgacacttta gcaacgacca caaacctcat cgacgcttta atgtatgaca ccaaagatgc
     3661 cgatgctaca gctttaatgg ctttgttagg agtttctgtt caatatgacg aaggacaaaa
     3721 cagccttcaa accacccaat ctattcaaag aaaaaatgac ggaacctatg aaaccaaaaa
     3781 cccaactcct ggagctaata atgatggaag tggaatcatt ttcaacggaa tttcaataac
     3841 cgcaaatact gtcaataaaa atgaaggcga tagttttcca ataactttca caatccaaac
     3901 caatgtaacc agcgatttga catttacttt tactttaaat aattcttctt tcacctctgc
     3961 cgatttttct ggcaatacta ctgttttaat tccttcaggt tccaatactt tcaccacaac
     4021 tatcacactt attgacgatg ttttgaacga aggcgacgaa gttttgaaaa ttaaaatagg
     4081 tgcaattcct gttcaataca aaaaactcaa cgacaatatc gaaattcgag tggtcgataa
     4141 tgattttacc gttgctccat tcggaacacc tttaaatcca acctatggca ttgtttccag
     4201 tacagctcct gcgggatatt atgcctcgct tgaaggactt tcgggagctg ctttaaaaca
     4261 agccattcaa gatattattg ccaatccagc tgttgttcat gctcataatt atggcgatat
     4321 tattgatatt ctaaaaaccg ccgatcaaaa ccctttgaac agcaacgaag tttggttgat
     4381 gtatgttgaa caaggccgtt ccaaactaga atttcaagac accggaatca atactggaaa
     4441 atggaatcgc gaacacattt atccgcaatc gcgtggcggt tttaccgatg gaaccgaaag
     4501 tattccagac ggaattaatc tttggttgcc gactaatgcc gacgatattc ttgccggtca
     4561 cgccgatgcc catcaccttc gtgccgaaga tggtgccgaa aacagcctta gaaataacaa
     4621 cgattatggc ttaacaggct acaacggtcc cagcggaacc aaaggttcgt ggaaaggcga
     4681 tgtggcgcgt tccgtttttt atatggcagt tcgctacaat gctttgagta ttgtcaatgg
     4741 agatattgcc gacaccaccg ttgggcaatt gggcgatttg gcttcgctct taacttggaa
     4801 tacgttagac ccagccgatg attttgaaat gaacagaaat aattatattt acacttggca
     4861 agtcaaccga aatccgttta ttgattatcc cgatttggcg aattatattt ggggagcaaa
     4921 agctggtcaa acctggcatt tcaatttgtc gaatcctgat tttgtgaatt taaaagtgac
     4981 tctttatcct aatccagccc aaaaatcgat tacgatttct ggtttgaatg aagaggctaa
     5041 catcgaaatt tacaacagcc ttggagcaaa aattttggag cagaaaattt atggagaaac
     5101 aaaattagcc attgattttc aaactggagt ttattatgcc aaaatccatt cagaggacaa
     5161 aacagttgtc aaaaaattac taattgaata aaaatatgat tgctattaga aactttttta
     5221 aaaataacaa tgctgttttt tataaacttc taatccttat ttttgttttt caatctataa
     5281 atttccaagc ccaaaccagt atgattacac cagcctattt acaaaaagga gacaccgttg
     5341 ccattgtgtc aacagcccga aaaaatattg atgacaatct gaaaccttca atagatttat
     5401 tgcattcttg gggattagaa gtcgttatag gaagcacgat tggattggat tataatcaat
     5461 tggctggaac ggatgaacaa cgtgctgccg attttcaaca acaaatggat aatcccaaca
     5521 tcaaagctat ttggtgcgtt cgaggtggtt atggcaccgt tcgaatgata gacttattag
     5581 attttactaa attcaaacaa catccaaaat ggattgtagg tttcagtgat gtaacggttt
     5641 tgcacaacca tctcaatacg atgggattca aatccattca cggcacgatg cctgttagca
     5701 ttgcaactac agcccccgaa tctaaagcgt ctttgaaaag tgctttgttt ggcgaaccga
     5761 tatcatataa aattaatccc tttccgatga atcgttttgg caaagctacg ggcgaattag
     5821 ttggcggaaa tttgtctatt ttaaatagtt tgttcgggtc taaatcagcg attgactgta
     5881 ccgacaaaat cttattcatc gaagatttgg acgaatacct ctatcatatc gaccgaatga
     5941 tgatgaacct gaaacgcaac ggctgtcttg aaagtatcaa aggaattatt gtgggcggaa
     6001 tgacaaaaat gaaagacaat gacattcctt ggggaaaaaa tgccaatgaa attgtgcaag
     6061 atataaccca aaaatacaat atcccaattc tctataattt cccagcagga cacgttcacg
     6121 acaacagagc cttgattatg ggaaataccg ttagtttgga ggttaatgaa aatggtggta
     6181 cggtggtttt tgagtaaaga gaatagaaga aagagtaaag aggatagact ttaaaaggtt
     6241 gattccgatt tcttttttct ttgctcttta ttctattttc tgaaatctaa aatctgcatt
     6301 ctaaaatcta aaatttctat ggctcaacac aacgaattag gaaaactggg agaagaatta
     6361 gctgttgaat ttcttcagaa agaaggctat gaaattctgg aaaccaattg gacttttcaa
     6421 aaagcagaaa tcgacattat tgccaaaaat gaaaatacac ttgctattgt tgaagtaaaa
     6481 actcgctcat cgttagaatt tggtttgccc caagattttg tgaaaccaaa gaaaattcaa
     6541 cttcttgtaa aagcagtaaa cgaatacgta atttcaaaaa atatagatat tgatgttcgt
     6601 tttgacatta tcgccatcca caaagaagac aaatcttttg taattgagca tcttaaagac
     6661 gctttttatc atttttaata aaaaaattta atgttatatt tgtaacaaat tgttttttat
     6721 ttatctttgc agaagttttt aaaccaaaaa ccaagtacaa actaagtaaa aacacctatt
     6781 ttgccatgaa aactgtttcc tctattgtag aaaattacat caaaacaaag ccatttttac
     6841 taaatgcttt gtcgcttgga atcatcaatt tgacctcttt gtccagaaat attatggttg
     6901 aattggaatc agaatttgga aaagaagtca aacaaggcgc tgttgtaatg gctttaaaaa
     6961 gactaaccga agagttggat tttaggctga atcacaagat caataaggtt atcaaaaaca
     7021 ttggcgaaat caccgttcgc tcttcgttga ccgattatac ttttgcagct tcggatactg
     7081 ttttgaacaa acaagccgat ttaattaccg atatcaatac tttttcggat atattttata
     7141 cctcatcaag aggtgtaaat gaaactaata ttgtagtgag caacaccgta aatcatttgg
     7201 ttgacaaaca ttttgccaac gaaagattaa ttcaaaaact agaaaattta gcttcgatta
     7261 ccgttaaatt accgaaagaa aatatagttg ttcctggaat ttattatttt atttttcagc
     7321 gtttggcttg ggaaggaatt atcatcaatg aagtcatttc gacttcaaac gaattcacta
     7381 ttttggttag cgaggatgaa gtagatatcg cattcaaagt gattaaagat ttgaaaaact
     7441 aacaactttt ctttttttat atagaaacga ggccatagtg cttcgttttt ttttgttaaa
     7501 attaaaaatt ataataagaa ctttctaatc cctaaattta cattctaaaa atagaacatt
     7561 tcccatgtta gctcctgctt atcaaaaatt aaaagataat ttatcaaaat ctattgatcc
     7621 caaaagaatt ttggacaatc cgttgcaaac ccttgcttac ggaaccgatg ccagttttta
     7681 tagacttatt cctaaaattg taattctagc tcacaacgaa acggaaataa tcgaaatcat
     7741 caaacaagcc aaagtattaa atattgcctt gactttccgt gctgcgggaa ctagtttatc
     7801 aggccaagcc attagtgatt cggttttagt ggttgccact cacggttgga aaaattttga
     7861 acttttagaa aacaatcaaa aaataaaatt agaacccgga attgttggag caagagcgaa
     7921 tacattttta gcacctcacg gattaaaaat tggtcccgat ccggcgtcaa ttggtgcagc
     7981 aatgattggc ggaattgttg ccaataatgc cagcggaatg tgttgcggaa cagctcaaaa
     8041 ctcctatcaa accatagccg atatccgaat tgtattaaac gacggaacta ttcttgacac
     8101 cgcaaatcaa gatagtgttg ctgctttcaa aaacaatcaa aaaggcttaa ttcaagaaat
     8161 agaaagccta agagatacca tcaaaaacga tgaaactttg tatcatttca tcaagaataa
     8221 attcaaaata aaaaacacca caggctacag catcaatgct ttggttgatt atcaagatcc
     8281 tattgaaatc atcaaacatt tgatggttgg atcggaaggg actttggctt ttatatccaa
     8341 cgtaactttc aaaacagtca ttgacgaaaa gtacaaatct tgctctttga ttattttcaa
     8401 cacgattcag gatgcctgca atgctactat tttattaaaa tcaagtcctg ttgctgccgt
     8461 agaattattg gatagagaat ccattcgttc agtcgaattt gatccagatg cgccaaccta
     8521 tttcaaaaca ttaccagaat cagcctgtgc tttattagtc gaatgtcgag acaatgattt
     8581 ggaagttata catcaaaaac aagaacaaat tcgactgcaa attcaatcca ttccaaccta
     8641 tacggattat gaatttacga gagaccccaa acaatattat ttcaattgga aagctcgaaa
     8701 aggtttatta ccaacggttg gcggactccg aaaaaacgga acttcggtca tcattgaaga
     8761 cgttgctttt ccattaccac aattggctga tgcatgtttg gccttgaaag atttgttcaa
     8821 gaaatacgaa taccacgatg ccgttttatt tggtcacgcc ttggaaggaa acttgcattt
     8881 tgtattttct caagattttt caaaccaaac cgaagtcgat cgatatgaaa aactgatggc
     8941 tgaattggcc gatttagttg tgaatcaatt taatggttcc ttaaaagccg aacacggaac
     9001 aggtcgaaat atggcgccat ttgtggaaaa agaatgggga actgctgctt acgaaatcat
     9061 gaaacacatc aaaaacatct ttgatcctca taataaaatc aaccccgatg ttttgattaa
     9121 tcctgatcca aaagcacatc tcaaaaacct aaaaccaatg ccagaatctc accatatagt
     9181 ggataaatgt atggaatgtg gcttttgtga accgcattgc gtttctgaag gattgacttt
     9241 atcacccaga caacgtattg tcattgcccg agaaattagc cgtttggaag aaacgaatga
     9301 agacccaact cgattagcgg aaattcagaa agatgtcacc tatcaattag acgaaacctg
     9361 tgccaccgat gggctttgtg ccttggcgtg cccagtaaac attgacacgg gaaaatttgt
     9421 aaaaacttgg cgtgccaacg aattaaaatc tggcaataaa aaagttgctg cttacattgg
     9481 ctctcatatg gcaggaacta cggcaatttt gagagtggga ttgaaaatgg tttccttttt
     9541 tcattccatt tttggcacta aaattatgac ggctttatcc aatggctctc attttataag
     9601 ctttgggaaa attccaaaat ggattcccga aatgcctaaa ggtgcccata aaattaacac
     9661 caaaaactga ccatagattc tcattgataa aatcaattat ccccaaagtc acaagtttaa
     9721 agttataaag tcttgctgat tgtagaaaaa ttgtgtctaa taaatgtacc caaaatactg
     9781 aagtagaaaa tccaattagt ctcaatttag taggaacttt tgaccttaga cttttgactt
     9841 gccactttta ggtactaatt aaaatttgat taatcctttt ttttgctata cattagaaaa
     9901 atattagtta ttctttaagt atattatctt gaaaaaaatt gttttatatt ttgtcttcgt
     9961 cttttcttta gctggttttt cgcaagaata tccggttaaa tttcttgata tatccgatgg
    10021 gctgtccaac aattcaataa ccgcaattca tcaggatagt gatggattta tgtggtttgg
    10081 aacttatgat ggactcaatc gatatgacgg atataatttt aaagtatatc gcaatagaat
    10141 caacgacaaa aactcattgt cgtttaatac tatttactgc attgagggcg attcaagaaa
    10201 aaacatctgg gttggaggtg caaatggcgc ctgtgttttc gataaaaaga aatctatttt
    10261 tcaccccatt gaatatcttt cgagtactaa aacacaaact gtaaaagatg tcattcacca
    10321 gattcgatcg gtttcaaaag atttggtgtt ggtggcttct caaaattttg gtttactggt
    10381 ttataaaaat ggctcatttc agggcaaatc aatagcattg gaacaatcca ataatccaat
    10441 tgaaaaatac aattataatg cagctgttct tgaaaatgat tccaaaaatt cttcttgttg
    10501 ggtatttgta agaaatatag gtatttgcaa atatagatat cattccaata agttggaagt
    10561 tgtttatccg ctttcgatgg aggtaaaatg tatgaaactc tctgtagata atacactttg
    10621 gttgggaacc gatgaaggga ttttttcttt taataccaaa accttctctc tttcggcaaa
    10681 ttacttaata aataactgca ccgttactga tattgttatt gacaagaaaa aagaactgtg
    10741 gttgactacc gatggctatg gtatttataa aataatcgat aaaaataaaa aagcgtttcc
    10801 attccagttt gccaacggac aacaatatat aaaaagcaat tcgatttgga gtttgtatga
    10861 agatattgca ggaaataaat ggattggaac tttgcgtggt ggaataagta tgattggcaa
    10921 cagcccaaaa tactttaaaa atttcaaata caatgtaaaa gtcaatgaac ctgccgaaaa
    10981 ttttattctg tccttttgtg aagatgagaa aaaaaatcta tggattggta ctgatggcgc
    11041 gggtttgaag tattgggaca gaaaatcaaa tagttatacg atttatggca attcgaacaa
    11101 ttcgacttat aaaattagca gtaattttat taccagcata ataagagata ccgacaatca
    11161 gatttgggtg tccacttggg ctggtggcat caaccgcatt aatcccgtaa cccatagtgt
    11221 cactcggttt tcgtgttaca actcatttac caaacaaatc gaaaaaaatg tctggtttgt
    11281 ctataaagac tccgatgacg atatctgggc cagcgctacc aatgaaggtt ctttgtactt
    11341 atttgatagg gctaaaaata catttgtact ctttaataaa tcaatcgata atttgcaatg
    11401 ccttattgag acgagcgacg gcaaattttg gggatgtaat tatacttctt tgttagccat
    11461 tgacaaaaag actaaaaaat acagaaaatt tccaattggt aattccataa ggtctcttca
    11521 tgaagacaag gacaaaaacc tttgggtagg cactcaagaa ggtggattgc tgttatttga
    11581 cagaaaaaca aataaattta atcgtttaac tacagacgat ggtttgcctt ctaatacaat
    11641 tttgagaatt ttagaagaca aagccggaaa tttatggatg agtacctata atggcatttg
    11701 tagatttgat aaaaagaacc gaacatttcg aaatttttcg gtaactgatg gcttgcaaag
    11761 caatcagttt agtttcaatg caggacttaa attatcttct ggagaattcc tgtttggagg
    11821 aataaacgga ttcaactcct tttttccaga agacatcaag gaattcaatc aaaagtacaa
    11881 cttgctgcta tcggattttt atgtaaacaa tcaatctatt gaagagagta aaaatgaact
    11941 gattacaaac tgggattcgg gtaaaatcaa ggaagtcaat ttgccttatg accaaaccac
    12001 cttgtccata gaatttgttg cattggatta caataatgcc gataaaataa attatgccta
    12061 ttatttagaa ggctgggaca atcgatggaa ttatgtaggc caagcccgaa aagcaaatta
    12121 tgcccgattg acagaaggga cttatgtttt taaagtaaaa acaaccaact ttagaggtgg
    12181 atggaataaa gaggaaaatc tggttaccat tatcgtctcg cctccttggt atagaagttg
    12241 gtgggcttat accttttatt tactggctgg agcaggactt atttatggtt atgttagata
    12301 caacaaaaac aaagaaaaat taaagtataa agtaaaaatt gccgaattag aaggagccaa
    12361 agaaaaggaa attgccgaaa aacaatcgtc aatgtttact tatatctccc acgagtttag
    12421 aaccccattg tctttgatta tcaatccgct aaaaaaagca attaaaacag gaaatttaga
    12481 aaacagttcc tcagtaactg atttagtcat tgcccatcga aatgccagac gccttttgag
    12541 tttagtagat cagttgttgc tttttcgcaa ggccgaaaat gatgccgatg aactcaaaat
    12601 gtctgtcata aatgtcaata atttgtgtta tgaagttttt caatgttttg taaatcaagc
    12661 caaagacaaa aaaattgatt atacttttca aataccagaa aacaacattg aaattattgg
    12721 cgattacgaa aaaatagaaa tatccttgtt taatttattg tcaaatgctt ttaaatacac
    12781 tcccgatgga agagaaataa gcttggtttt gtccgaaaca gaaacaaatg tcagcataga
    12841 aattatagac agtggtaatg gaatcgcaaa aaatgatttg gaagttattt tcgacaaatt
    12901 caggcaagta aactctaaaa cgccagttgg tgggagctct ggatttggaa ttgggctttt
    12961 catcgtcagg tattttatag aaaaacacaa aggaactgta agttgcacaa gcgaaatagg
    13021 aaaaggaagt gtgtttaaat taacttttct gaaaggagaa gaccattttg agaatttgca
    13081 aattagtacc gttacaccca aaatgagtcc gttggtagag gaattgatgg ccgatgacac
    13141 cgaagcaggc attgaagttt cggttaatgc catttcagaa aatgaattgc ataaagcgat
    13201 acttaccgat aagcgaaccc ttttgattat tgaagacaat accgatatcc gaaattattt
    13261 aatcaggctt ttttcagagg tctatataat ttacagtgca gagaatggtg aagaaggttt
    13321 gaaactaacc aaaaaacaca tgcccgattt ggtaattagc gatattgcaa tggacgttat
    13381 ggatggtttt gaattgtgtc gaaaaataaa agaaaacagt agtctatccc atattccggt
    13441 aatattattg acggcttcaa aaaatcctga aacgcatctt caaggaataa gcgacggagc
    13501 agacgattat ataaccaaac cctttgacga cgatttgcta atggctcgtg tggaatcgct
    13561 attgaaaaat cgcagtaact taagagccta tttcttggac agtatcaccc taaaagaaaa
    13621 tacccagaag gttcctgccg aataccaaga gatgctgaaa aaatgtattg ccattatcga
    13681 agcaaatatt cacgaacgtg attttaccat caagaatttt gctactgaaa tgggaatgag
    13741 tcacagaaca ctttacacaa aaattaaaat catttctgga caaaccctaa atgcgtttat
    13801 ccgttccata cgcattcgaa gagcggccat gttaatgtta accgaaaaca tgaatattgc
    13861 ccaagcaagt gctgaggtag gttttgaaga cccaaaatat ttcaggcagc aatttgtaaa
    13921 attatttgga atcacgcctt cagaatacat caaaaaatac aaaaagtctt ttgattctga
    13981 tttgaatatc atcaactaat ttagaagaaa gaatccgcat tagaaattta tttgtttcaa
    14041 attctaatgc ggatttttta tgcttttatt tctctaagtc tataaattct gatttattga
    14101 aaactcgttt tttcataaat ttaagataaa aaaactcatt ctacatttaa aataataatt
    14161 acatactatt aaaaaaaaat tcattttttt tttacatttc atattttatg gtttaaaaat
    14221 tcagcttatt gcgaatttga cctgctattt tttccaattt acccctcatt aaactttaca
    14281 actagccatt actttgtgat tgttaattta gaatggcagt aacatctgtt aattctatat
    14341 taacggaatg ccaataaatt taaaaggctt ttcggttttt aataaatcga tatagttaaa
    14401 agagtattaa aaataaaaaa ccaattataa accatctaaa agaaagtagt atgacaaaaa
    14461 acaaacattc catttacaaa ccaatttatt gcaacaacaa ttgttaaaat tagactaaat
    14521 cagatcgttt ttaccgttca attataaaaa atctgatgaa tcattctgga aattaatctt
    14581 tccaaattaa tctaatgata gggcatttta aatcaatttg atatttaatt aaaattctat
    14641 tctagaaaaa aactaactat aaaaacctta aaattcaaga gatgaaaaaa atcacatttt
    14701 tattttttct aacctttttc acaatccttt atacaggttg tcaagataat gggccagatt
    14761 atccaagttc ggataatcca acagttgttg ccgctacaac agagatttac ggtgctccca
    14821 acaagaaatt tgaaattaaa gcagccttgg ccgatgattt aggcttaaag acagtaagaa
    14881 ttcaaatacc agaattgtct ttggataaag tgatcacctt tgaaacagat cctttgttga
    14941 caacctataa tttgagctat ttctttgaga ttcctgccga cagaggtaca tccgaagcat
    15001 tcaaaattaa gttgaccgta actgacgttt ccaataattc agtcgatcaa gaaattaaat
    15061 tgcgtttgga tggagagttt gatgctcctg ctgtaacaat gattactcca aaagagggag
    15121 cggttatcct attgtcaact agtaaccaaa tgccggtgaa ttttacagtt aatgataatt
    15181 cgggtatcga ttttgttgaa ttgaaatgtg atgcgctagg tatcaatgaa ctagttcaat
    15241 tacaaggagc tcctcaatct tatactttta acaaaacatt tactttgcct gttactgcag
    15301 ccaattatgt attgacaatt aataccagag ataaatttgc aatacccaat tcagggacgt
    15361 tgaaaatcaa tgttgtagca actaacgaat atcctgcact ctatttatgc gatcagccta
    15421 aaggaactaa ccttactact gatgctgtag gtgtgccaat gtattttcat gaaaaagttg
    15481 ggcaagattt tacattcaaa tattatgcag attcagataa taaagaggtc tatttcttag
    15541 gacaagaatc agattttgca ccacactgtt ttggtctaaa ttcttctgga aaattggcag
    15601 atgatgttgc ttcatcacca attgttttac caacaaaagg atattatcta ataaaagtaa
    15661 atccaaacac attactttat acagttacta aatatacccc aacaagtaaa gtttggtcca
    15721 atattgcaaa cggattatgg catgatgatg ccgctctaag aagaccattt gtaagtgttt
    15781 gtggtggagg tattcaagga gcaaattggg atacttggaa cgcctggaca caaaccagtt
    15841 tgcacttggc taataattcg aataatcctt atcaattagt tggagaatac acgcttacag
    15901 gaactatgga gtgtactttc acaggccagt ggtgggatcc gtcttggaga ttaatcaaga
    15961 atggtattgc aacgatgtca ccaggaggaa atggaaatgc taaatatcca gcagcaccag
    16021 gtgtttataa agtaattgtt gacactgaat tagaaagagc ctttataaca aaaaaataat
    16081 atgttatgaa cttgattgta acatttaatt aataaaaata ttttaacatg aaatttaaac
    16141 atatatggtt atttattgcc gtgttgtgca gcgcaacaac atttgggcag ataacagtga
    16201 aaggaactgt taaggacaat aaagccctac cgattcctgg agttaatgta tcagtaaaag
    16261 gaacaactac atcggcatca actgatttgg atggaaaata tgccattata gttccaaaca
    16321 aagaggctca actcgaattt tcttttattg gtttcacaaa taaaattgta aaagtaggag
    16381 ataaaacact tgttgatgta atgttggaag aatccaatca agcattagac gaggtcgtag
    16441 ttgttggtta tgccactgtt aagaaaaagg atgtaaccag ttcaattgct tctgtaaaag
    16501 gaaaagaact tcaaacaatg actgttggta atgctactga atctttgcaa ggaaaagtgg
    16561 ctggggttca aatcacagga gctggaggac caggcgcaca acctagagtc ctgatacgtg
    16621 gaatctctac ggtcaacctt agtacagatc ctctttatgt ggtagatgga atcccaatgg
    16681 gaacaagtat caatttcttg agcaataatg aaattgaatc tatggatgta ttaaaagatg
    16741 cttctgctag cgctatttat ggttcacgtg cttcgaatgg agttatcctt attacaacca
    16801 aaaaaggaaa agtaggtaag cctaaattca atgtcgattt gagtaatggt cttcaaataa
    16861 tgaataaccc ttacaatatg gcagatgctg aaagttatgc caatatctta aacaaagcct
    16921 atactaattc tggatattct gaatatttgc caaatgctga acaatataga ggaaaaacaa
    16981 ccgattggtg gggagcagga attaacaaaa cttctcaagt atccaatgct tctataggag
    17041 ttagtggagg ttcagataaa aatacttatg ctatctttaa attactataa ttccgattcc
    17101 ttttacaata ttggaggatg ggaaagagtt actgccagaa ttgctaacga tttcaagttt
    17161 tcggacaaaa tttctatggg gattacttta aatccacgtt atgaaacttg gggttcacct
    17221 ggtaactggg ccgattttga taaaattgat cccattaccc caatttacaa accagcagat
    17281 caattgaatg gtaccgaaaa tgagtatagc atctatgcac gttctccttc gtatgtgtgg
    17341 aatccagtgg cttccgtagc tagatatgat gattacacaa atttatacaa cttgaacacc
    17401 aatgcttatt ttcaatatga acctatcaaa ggattagttt tccgtacgca aggatctatc
    17461 gaaatagggg ataaaacaca aagtatcttt agacctgatt tcgagattga tgcagcacac
    17521 gaaaaagcag aaatcaatag tgttgaaaga aaaaacacga ctaatcacga ctggacttgg
    17581 cagactacgg ctacttattc taaaacattt gctcaaaaac acaatgcttc tttaatggtc
    17641 ggtgcaacaa tggaggaata caatggaaat gatgtttggg gatatggaga aggagttcca
    17701 aacaactctg atttgatgag agaagtaaat gccgcaacca aaaaccgtaa cagtggcggt
    17761 aatagttggt caagttcttt acagtcctat atttcgcgtc tttcttataa ttatgatggc
    17821 aaatattatt tgacaggaac gttcagacgt gacggttctt caaaatttat ggccaataac
    17881 aaatgggcca atttcccatc ggcttcagcc tcttggagaa tttcgaatga aggctttatg
    17941 gaaaacacaa aagcagttgt aaatgatctt agattaagag ccggttgggg taaagttggt
    18001 aatcaaagtt taccaggttc tgtttaccag tctaatattg gccaaggata ttacgttatt
    18061 ggtaatgagg ttgtggatac ttcttatcct tcttcaatgg ctaacaaaga cattaaatgg
    18121 gaaactgtag aggatattaa ctttggtatt gatttcggac tatggaaaaa taaattatct
    18181 ggatcaatag aatactacga aaaaacaaca gaagatatgt tgttcaaaaa acaatttcca
    18241 acttacagcg gtttccctgg atatgcaaca atatggacca atgtaggatc aatgaaatca
    18301 aatggtattg atgtgctttt atcgtacaaa gacaaaaaag gagatttctc atatggagcc
    18361 gactttactt ttaccacatc caatgtaaaa atggttagct tgtctgcaga tggagaaaaa
    18421 ttatatggat caagtaatag aaccttaacc atcgaaggtg atgaacccgg atattattac
    18481 ggatatatag ctgatggttt gtttcaaaat caaaccgaac tgaactctca tacagatgaa
    18541 cacggaaata aattacaacc ttatgctcaa gctggagatg ttcgcttcaa agatttgaat
    18601 aatgatggca aaatcgatga caaagacaga acaaaattag gttcgccttg ggccgatttt
    18661 actatgggat tgaatttaag tgttgcatac aaaaactttg atttgatagc caacttctac
    18721 gcaagtattg gaaatgaatt gataaatcaa aatatttcag atttgtataa tggtgccagt
    18781 ttgaccaaca aagtagctgg actagatcaa atggcttgga atggagaagg aacttcaaat
    18841 tatgttcctc gattgtctaa aaatgacaat aacgaaaact tcaccaaatt ctcttctttg
    18901 tatgttgaag acggttcctt cttgcgtatg aaaaacattc aattaggata cacgttcctt
    18961 aatcaatttg gacttgataa attaagaata tcaatatcag gtcaaaattt atggacactt
    19021 actaactata caggtgtaga tcctgaagtt ggtggtggtg ttttgggttc aggatttgga
    19081 ggatggaatt atcctgttca accaactgtt ttgatgggta ttaatgtagg attttaataa
    19141 aaatgaaaat gaaaaaaata ataatatcga taatcgcttt ttctctattg gcagtttctt
    19201 gcgaaaattt tattgaaaaa gaagaaagag gaacacaaac tttagaaaac tactttcaaa
    19261 ccgcacaaga atgcgagaat tatgttaatg agttaacaca aaggatgtta ctgtctaaag
    19321 attggtacac attgcttgcg ccaagattaa ttaacgaaac ggctagcgat gatgcctgga
    19381 tgggcaacac gggtcaggac aacagtgcgt ttagaccagg tgctcagtat gtcattacac
    19441 ctgaaaatat gggcgctttg aatagtcttt ataccgcaca ttattacacc attcaatcgg
    19501 cgaatattgg tttagagaaa atggcaaata ccccaattag cgaatctcaa agaaaccaat
    19561 atatgggaga atccttgttt gttcgtgctt attgctacta tgagttggta aataattttg
    19621 gagcagttcc cttgtacaca aaatcgttag gaactaatga tttaaaatta gaacgtagcc
    19681 ctgttgccga cgtttatgca caaatagaaa aagatctgaa agaatcagca accaaactgg
    19741 aaaatattcc tgttgataga aacggaagag tcaacaaatg ggctgcttat gccttattag
    19801 cccgtgtatc tttgtttcaa gaaaaatgga ctgaagcaaa acaatattcc aacaaagtga
    19861 ttacagaagg cccttttgct cttgaagcca atttcttgaa catctggaat gtgaacaatc
    19921 acaatggtgt cgaatccatt ttggaagctc aatcctcttc ggtacaagat aagagtttag
    19981 gatctatgtt gcctaccttt tcaggagcaa gaggcgaaga caagaaaaac tttccaagca
    20041 atgatgccaa agatgttatc gatggttggg gatggtgtat gccaaccagc gatttagaaa
    20101 acgcctatct ttcagaaaat gatgtagtac gtcgcagaag caccatcacc aaatggggag
    20161 aagctgctta tggcgatgaa gttcttaatc ctactcacaa atttagcttg aatgacaata
    20221 aatcaggtcg tatttgtcgt aaatactata ttcctatagc aacccgtcgt gcattggaca
    20281 aaaaagatgg acatttacca ctaaacgtgc cgttgattcg tttggcagaa atgtatttaa
    20341 caagagccga agcaaattat caattgaagg atgagccagg agctttaaat gatatcaatg
    20401 tgattagagt tcgtgccaaa cttgacccaa aagtaggatt gttagggcca aatcttttga
    20461 gacaaatcta caaagaacgt cgtttagaat tggcttttga aggattgcgt ttgtttgaca
    20521 ttcgtcgtga aaaagaccca agtacttcaa aaccagttat tgaatcctta ttgggaccaa
    20581 acggaacttt tgtgaaatac aacttgagtt ctacagatcc ttttgaaact accaacttga
    20641 aagaatctca agataaagga atcaatttca atccggctaa aaacttattg tggccaattc
    20701 ctcagttgga acgagattta agtggtggat taataacaca aaaccctggt tactaacatg
    20761 attaatatta agaactataa agtaagcctg ctatttgcag gtttactttt aattaactcc
    20821 tcattgacaa gctgtagctc atctgataat acagataata cacagcctcc aactacaaca
    20881 acgagagatt tgaatgtaaa tctggatttg aaattgcaaa caatggagag ttttggagca
    20941 tcagacgctt ggcaatgtaa ttttattggt aaaaattggc ctttggataa aaaaaatcaa
    21001 atcgccgatt tattatttag caaagatgtc gatgcaagcg gtaacccaaa aggaattgga
    21061 ttgtctttat ggcgtttcaa ccttggagca ggaagttcag aacaaggtaa cgcaagtgat
    21121 attggagatg agtggagacg taccgaatgt ttcactaccg acggaattag ttatgatatg
    21181 aacaaacaag caggtcaagt ttggtttatg aaagccgcaa aagctcgtgg tgtcgaaaaa
    21241 ttattggctt tcaccaacag tgcgcctgtt tacttaacca ataatggtaa agcttacgca
    21301 agtatcaaag aattttacaa tttgaaagac ggaaaaatgc ccgaactagc tgatttttgg
    21361 gtaaattcta ttgataaatt gaaaacagtt cacggattga ccatagatta cgttagtcct
    21421 ttcaatgaac cacaatacga gtggaatggc acaaatcaag aaggatctcc cgcaaccaat
    21481 gccaatattt acagttttgt aaatgccttg tcgccaaaat tactggccaa aggattgaat
    21541 tcccaaattg tagtaggtga aggaggcgca tttgaaccac tttataaaac ggtatctggc
    21601 acagagagta gatcaaacca gatcgattat ttctttggaa caaattctac caaaaacata
    21661 ggtggattaa gcaatgtaaa gaaaactatt tcagcacaca gttattggca agcttggcca
    21721 ttgagttcac tagtttcttc aagacaatct gcagcttcta aaatacaaac agttccagga
    21781 ctcaaccttt gggcctctga atattgtatt ttagaaagtc caggaaccgc cgagttacct
    21841 ggtggagctg gttccggaag agatttagga atgcaaacgg ctctttgggt ggctcgtatt
    21901 gtaagtagcg acattgccat cggaggagtt tcttcttggc aatggtggac ggcagtaagc
    21961 agaggcgact ataaagacgg attaatccac gtagatgatg gagccagtaa cggagcggga
    22021 ggcgcagatt attgcaaaaa tgatggatac gttagagaat ccaaaatact ttgggcttta
    22081 ggaaacttct ctttctttgt aaaaccagga atggcaaggg ttcaaatacc aaatattgac
    22141 aactcaacag ctacaaccga tgtaatgctt acggcttata aagacgaaac aaataaaaaa
    22201 ttggtcatcg tggcagtcaa ctttagtaaa acggctagaa cctacaaatt gaatctttca
    22261 ggaggaacgt taacgaataa taagttaaca ccttacacaa cctccgatgc tttgagtttg
    22321 aaaaaaggaa cagatgtaga tcctgccaat ttcgaaatag gcgctagatc cgtggtaaca
    22381 tacgttggaa cgtataaata atttttaatt tggttagtta gtagttttgt ttggtgaata
    22441 aggtttgtct aattttttag gcaaacctta attcattact ttcctaataa tgggaactat
    22501 tttatccttt tttaagattc caaaatagac caagatcagt tagtaccttt gtaggaagtt
    22561 atgattaaat caacaagcta attaaaaatt attttagaag ttaaatcaat tggaatataa
    22621 aatgaaaaac acctacatca cttttctttt gttgttaagt tcgttggctt ttgcacaacg
    22681 aacagccacg ataaacacag ataatgtgtt gcaaacgatg gatggttttg gagcttccga
    22741 tgcttggaga tgtcaatttg ttggaaaaaa ttggcctgac caaaaaaaga atgccattgc
    22801 cgatttattg tttagcaaag aagtcgatgc acaagggaat ccaaaaggaa ttggactttc
    22861 tatttggaga ttcaacttgg gagcaggaag tatagaacaa ggggaaaaca gtaaagtttc
    22921 ggatcaatgg agacgaagtg agtgtttttt gaacgctgat ggaacatacg attggtccaa
    22981 acaagaaggg caaagatggt ttttgcaagc tgccaaaaaa cgaggagtag aaaaatacct
    23041 gatttttacg aacagtccgc cagttcatat gaccaacaac ggattgtctt ttgccacaca
    23101 aaaaaataaa ctgaatttaa aagaaggtac aattccaaaa tttgccgatt ttttggttca
    23161 aagtattcaa ggattggaga aaaaagaagg aatgaaattt gattatgtga gtccaataaa
    23221 cgaaccacaa tgggaatgga tgcccaacaa tggggatgaa aatagccaag aaggaactcc
    23281 ggcaaccaat caagaaatat acgatttgac caaagcactt tcggataaac tcaaatccca
    23341 aaaaatgaat actgaaattg tgattggcga agcagggcaa atcaattatt tatatgaaaa
    23401 tgtaaatgac gaaaaccgtg acaatcaaat cgattacttt tttggtaaag gaaaaacgaa
    23461 tatttcaaaa ttgcctaacg taagaaacac catttcggga cacagttatt ttacgacttg
    23521 gccaattgac agacaagttt taagccgaaa attgattgct gccaatgtca aacaaaaacc
    23581 gggattaaaa tattggcagt cggaatattg cattttggaa aatccgggag aaactgaaat
    23641 tccaggaggt tcaggaggaa caagagattt gggtatgcca acagccttgt ttgtagcccg
    23701 cttgatacac aatgacgtgg ctcttgccaa tgcggcttct tggcaatggt ggacttcgct
    23761 gactcgcgcc gattataaag acggtctgat ttatttggat gacggaaaaa gcaatggagg
    23821 aacagctcct gattatgtta aaaatgatgg ggaattccac gattcaaaac tactttgggc
    23881 tttgggcaat tactccttgt ttgtgcgtcc gggaatgtta agagttgacg tgcccaacca
    23941 agatgaattg gcttctgcca atgatgtgat gctaacagct tacaaggatg cggcaaacaa
    24001 aaaactaata attgttgccg taaattgtgg cacatccgat cagaattaca aatttaattt
    24061 gtcaaaagga acgctcaaga acaatgaact gacgccatat attacttccg aaagttccaa
    24121 tttaaaaaaa gcagccctcc agaaaataga caccattgta attccagcta aatccgtggt
    24181 cacttttgtg ggtgaatttc agtaaaacag tagtatttag aagtaacaga ccttttataa
    24241 aatgaaaaat aaaaatctaa tctgcctctt gattttgatt gtttttggta gtatgatgaa
    24301 tgcccaaaaa tcaaaaaacc aagtgtcaaa cctaaaaaca acggcctatt tatttgccta
    24361 cttcacggga aattcaaaca atgaagaagc cattcgcttt gcagtgagca acgatgggta
    24421 caacttcagg gcgttaaatg gcaatcaacc tgttattgat tcaaaggaaa ttagttcgtc
    24481 tggcggaata agagacccac atattcttcg tggagccgat ggcaaaacgt tctatatggt
    24541 cgcaaccgat atggtttctg ccaagggttg ggattcaaat cgagcgatgg ttttattgag
    24601 atctactgat ttggtcaatt ggaactcatc tgtaataaat atccaaaaaa aatatccaaa
    24661 ccaagaaaac ctgaaacgcg tttgggcgcc acaaaccata tacgataccc aaaaaggaaa
    24721 gtatatgatt tattggtcaa tgcaacacgg aaacgatatt gacaaaattt attacgctta
    24781 cgccaatcct gattttaccg atttggaaac ggaaccccaa caattgtttt tcagtccaac
    24841 gaatggagct tgcattgacg gcgaaattat tttcaaagac aataaatacc atttgttttt
    24901 caaaaccgaa ggttcgggag cgggtataaa aattgccgtt tccaacaaac ttaccgaagg
    24961 ctatgttttg agagatcaat atgtgcaaca aaccaaagat cctgtagagg gtgctggagt
    25021 tttcaaactg aacaattcca atgactacat cttgatgtat gatctttata ccaaaggaaa
    25081 ataccagttt accaaaacca gtgacctaga gcatttttcg gttattgata acgctgtcac
    25141 gatggatttt catccacgtc acggaaccgt tatgcccata acggcaaaag aattagcccg
    25201 attggaaagc aaatggggaa atacgaaaca ataaaataat tgagagctgt atctcaatag
    25261 aacattagaa acagcattat ttgattaata gaataacata aattacacat aatgattaca
    25321 aagaaaatct tattaccaat ttttattttt tcctgtctat cggctacaag ccagatatcg
    25381 ttcggggatt ctcaaaaaat taacaacaac tggaagttca cgcttcagga cactccagaa
    25441 gcatcaagcg aaacctacaa ggatactaat tggcaatcgg tcaatgtacc acacgattgg
    25501 agcgtcaagg gcagattgag tcctactttg gctagttgtg ctggtttttt gccgggagga
    25561 attggttggt accgaaaaac attgaatatt ccacagacaa aacgaggcga aaaagtctat
    25621 ctgtattttg aaggggtata taacagaagc gaagttttca tcaacggaca ttctttagga
    25681 aaacgcccca acggatacat ttcttttgcg tatgatgcta caccttttgt aaaatatgga
    25741 gaagataatg ttattgctgt aaaagtagat catagcttga gtgccgactc gcgttggtac
    25801 actggttcag gaatttatcg caatgtgtgg ttggtttatt ccaatccagt acatatcgct
    25861 caatggggcg tttatactta tccagaagtt gctaatggaa cagggatttt gaatgttgag
    25921 gtagctttag aaaacggttc gactgcaaaa acaaatctta ctgttgtcaa tgaacttttg
    25981 gacaataacg gaaaagtagt ggccaaaagt tcccaaaaag ttttagttga agccaataaa
    26041 gataataaag ttgcaaccaa aataaacttg aaaaatccca agttatggga cgtggagcat
    26101 cccaatttgt atcagttgaa aacaactgtt ttaaaagatg gcaaaccaat cgataaaacc
    26161 gtaacgtcaa caggttttag aaattttact ttcgatgcca ataaaggttt tgccctcaat
    26221 ggcaaatgga tgaagatgaa aggcgtttgt ttgcaccacg atgcaggagt tttgggttct
    26281 gccgtaccaa gagatgtttt gaaaacaaga ttacaaacct tgaaagaaat tggtgtaaac
    26341 gccattcgta ccagccataa tccacaagct cctgattttt atgccctttg tgatgagatt
    26401 ggattgcttg ttcttaatga agcgtatgac gaatgggaat tccctaaacg taaatggtta
    26461 gaaggttgga atgtaggtac tccaggattc cagggttcga atgatatatt tgctgcttgg
    26521 ggcgaaaaag atttggaaga tatggtacgt cgcgatcgaa atcatctttc cgtatttgct
    26581 tggagcattg gtaacgaagt agattatcca aatgatcctt attcgcatcc tgtattggac
    26641 ggcggaaaag ataccggatt tggtcaagct gcttatggtg gctataaaaa agatgctccc
    26701 gatgctatgc gacttggcgg tattgccaaa cgccttgtgg cagcagtaaa aaaatatgac
    26761 aaatcccgtc caaccacggc aggacttgca ggtgtggcaa tgtcaaacga aaccgaatat
    26821 ccgggtgttt tagatattac cggctataat tataccgaaa gcaagtacca gtccgatcat
    26881 aaaaaatatc caaaccgagt gatttttggt agtgaaaccc gccacgaaat cgaaccttgg
    26941 ttggccgtga aaaacaacga acacattttc ggacagtttc tatggacagg aattgattat
    27001 ttaggtgaat ccaatgcttg gccttcaaga ggtttctatt ctggattagt agatcttgcg
    27061 ggaattatta aaccgagagg ctatttccgt caatcattat ggtccaataa gccaatgatt
    27121 tacgcaggaa cttatcccgt aaacaatagc aaagaacttt ctatagacgc ttggcctttg
    27181 tggaattaca ataatggaca aacgattcga gtggtttgtt ataccaatgc cgcaaaagcg
    27241 cgtctggaat tgaacggaaa agtggttggc gaagaaaaaa aatacgatga aaaaactgga
    27301 gttatttatt gggacattcc tttctcctca ggaaaattag aagcaatagg acttgataat
    27361 gatggcaaag tggttagtca atacgcaatt acttcctcac agcagcctgt ttctttggta
    27421 atcaatgaaa aaaacatcac catcgacaag gaaaaagaag ttgctaaagt catcgtgcaa
    27481 gtattggacg aaaaaggttt accggttatg ctttcggata acgaactgac ttgtgtcgtg
    27541 agtggctcgg gaactttgtt gggactagaa gccggaaaca atgaagatat gggcgattat
    27601 acggacaatg ttcaaagggt ttttcacggt catttagtag cttatattca gtcaactgga
    27661 agtaatgagc ctataactgt caagtttaca gcgaactggt taaaatcggc tgaggttacc
    27721 attcaagtaa agtaagtttg tgtttttaca atgacttcat gatataactc attttaaaac
    27781 taactacata gacttttggt tgtctatttc cataatttgg tataagtgcc tgattttaca
    27841 aaacggatta gttagtaaaa acccgaaaag aatatttttc gggttttttg ttttaatctc
    27901 cgtatccaag aactaaattt aaaaaaggtt tttcttcatt tgtattggtc agaaaggcaa
    27961 tagtattgaa ttaaataatg tcaaaatttt ttattatcat tctttttttt agcatattaa
    28021 ttttttttta tgtctttttt taaattacat ttacaaatgt aaataataca cttgtaaata
    28081 aattatttta ctcataataa tcacacaaaa tgaatctatc taaattttcc aaaatagtgt
    28141 tcactatttt atgcttttca gcattcaatt ctgttcaatc acaagctcaa aaagagtttc
    28201 atagttgggc atccacacca ccaatgggtt ggaatagctg ggattgtttt ggtcccacag
    28261 ttactgaagc cgaagtaaaa gccaatgcca attatatggc aaaatacctg aaaccctacg
    28321 gatgggatta catcgtagtt gatatccgct ggtatgttgg aaatgataaa gcccacggat
    28381 acaacgaaaa agatcctgat tatgttctcg atcaatacgg acgatttatg ccagccataa
    28441 accgttttcc ttcagcagca ggaggaaaag gttttaaacc tttggctgat tacttgcata
    28501 aaaaaggctt gaaatttggt attcacatta tgcgtggtat tccagtaata gccgtaaaac
    28561 aaaatctgcc aataaaagga accaatttca ccgccaaaga catttattcg gataaagacc
    28621 aatgcaaatg gctacacgat atgtacactg tacttccaga aaaaccggga gcgcaagaat
    28681 attataattc gttatttgaa ttgtatgcct cttggggact cgattttgtg aaaatcgatg
    28741 acctgtcttc acccatttat tttgagggag aaatcgacat gattcgaaaa gccgttgatc
    28801 acacaggacg caaaattgtc ttgagcactt ctcccggaga aaccccaatt gctcacgccg
    28861 atcacgttca aaaaaatgcc aatatgtggc gtaccgttgg tgatttttgg gacagttggc
    28921 aacaattgaa agagcatttc gaagtctttg aacgttggaa caaatggcga gcttatggtg
    28981 cttatccaga tggtgatatg ttgcctttgg gacgaattgg catcagagcc gaaagaggag
    29041 atcctcgaat gaccgctttt accaaagacg agcaatacac cttgatgaca ttgtggagta
    29101 ttttcaaatc tccgttaatg tttggtggca accttcccga
//
LOCUS       DAOSNN010000005        28679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_1652_length_28679_cov_5.959719, whole genome shotgun sequence.
ACCESSION   DAOSNN010000005 DAOSNN010000000
VERSION     DAOSNN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 28679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28679
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1652_length_28679_cov_5.959719"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3745
                     /locus_tag="PLQ70_00500"
     CDS             <1..3745
                     /locus_tag="PLQ70_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HQE33124.1"
                     /translation="QDLLALANPLFHPTITTSWSASEGKLKLYSPTGIPVSYSDFEAA
                     IKDVTFTNSSASPSGTRDFSINLGFGSANYLPSNGHFYEYVASPGIIWTAAKDAAAAR
                     TYYGLQGYLATLTAADEAQLAGAQAPGTGWIGGSDAQIEGTWKWVTGPEGLANGGTGI
                     TFWTGKGNGTTTPPFNYANWNAPNEPNDSGNNEDYAHITSPAVGNLGTWNDLPVQGNA
                     INVANGNYFPQGYIVEYGGMPGEPTLQISASTKITIPKITSSTPSAICGSGSLILQAT
                     ATDGTVNWYTTPTGGTSIYTGNSFTTPVLTTTTSYYVDASNGSCPNGPRTEIIATIKP
                     LPNLPQIAISSTSPVLYCLNATAIPLAATADSNCKLNWYTQAVGGIASATSPTPITTT
                     AGTTNYYVSQTNTLSNCESLRAAIIVTVNPLPTVPTVNNISYCNNETAVPLTAIATAN
                     CTLNWYPTATGGTPSATSPTPSTIVVGTTSYFVSQTITSTGCEGPRAEIVVTVNPLPT
                     APIVSNIAYCNLETAIPLTATASPNCTLNWYAASVGGTSSATSPTPSTATIGTTKFYV
                     SQTIVATGCESPRSEISVIINPLPIVNDVTIIQCDADLVVDGKTLFNLTVNNAQISAN
                     YTNETFTYYTSLNGAKNAIPADLIPNELAFQNTTPTQMDVWSRITNKTTGCFSVAKIT
                     LKVPATNINPNTKIPFPAVCDDFLDANGINNANNNNRDGITTFDFSSTKAIIQALLPP
                     TDVYNINYYRNQADALAELNVISDISNYRNIGYPNSQDIWVRIDSNLDNACYGLGPYL
                     TLNVEALPIANTVTIPRQCDDNNDGIFTFNTLNLESTLLNGQSNKTVTYFDQANNPLK
                     DANGILIKSPFPATFTSTSQTIKAVVTNNSPQHCYDETNILFIVDQSPVAFAVPSNLT
                     TTCDDEPNPIDQDGKFAFDTATFESTILGGQTGMIVKYYDQNNMLLPSPLPNPFVTNT
                     QNITAQVQNPLNTTCTASTTLNFIVNPVPNIDLNLDGSANELVCQNDSAFFVTLDAGI
                     TDGSSTTNYDYTWTKDGVHTGTNSPTLDVNAEGVYTVEVTNHSGCSRIRTLTVTASNI
                     ASITSVDVVDLTEVNTITVNVTGPGVYEYSLDEPNGFWQDSNFFNNVPAGVHVVYVND
                     KNGCGSVHQEIVLVGVPKFFTPNGDGYNDYWSIKGVNETYNSNSIIYIFDRYGKLLKQ
                     WVPSLNQGWDGTFNGVPLPADDYWFTLKLEDGRETKGHFSLKR"
     gene            complement(3755..4891)
                     /gene="folK"
                     /locus_tag="PLQ70_00505"
     CDS             complement(3755..4891)
                     /gene="folK"
                     /locus_tag="PLQ70_00505"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQE33125.1"
                     /translation="MKSQNQVVLSLGTNQGNRLENIEKSIELIHQEVGTVIKTSKLYE
                     TPSWGFDSDAFYNCALVLHTFLSANAILTKILSIEKQLGRIRNTTQEYQSRIIDIDLI
                     TFNEEIIDSEDLQVPHPLLQNRNFVLLPMLDLNLNWIHPVLHKKTAELFEISPDESVC
                     VLVQSLKNPLDKIPLEQFNYIAFEGNIGAGKTTLATKIAEDFNAKTVLERFADNPFLP
                     KFYEDQNRYAFPLEMSFLADRYKQLSDDLAQFDLFKDFIVADYHIFKSLIFAKVTLQE
                     DEFRLYKTMFDIVYREMPKPDLYVYLYQNTERLLENIKKRGRSYEQNIPADYLDKING
                     GYLDYIKTQTNLNVLIIDVSDRDFVKNQEDYLYILDEIRRKISN"
     gene            5007..6767
                     /gene="sppA"
                     /locus_tag="PLQ70_00510"
     CDS             5007..6767
                     /gene="sppA"
                     /locus_tag="PLQ70_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HQE33126.1"
                     /translation="MRFLGNVLATIVGLFVFIMLFVFGIVLIGMLFGGESDEISVKSN
                     SVIELKLDEIKNDYAGKYNNPWVTEFSKGGKVGLSDVIKAIEAAKTDSDIKGISILND
                     ESELGMAQRKALRNALEDFKKSGKFVMAYANSYSQKEYYLNSVANTIYINPVGDFDFK
                     GLSAELMFFKDLQEKSGIKMEVIRHGKYKSAVEPFLENGMSDANREQTTSLLNAVWSS
                     VLADISKSRNISVAKLNEIASGLLARTPEMAKSEKLVDVIAYEDEYHDAIKKILKVDK
                     DEDYNKTAILDYAQKVATTSESNDSNDKIAIIYAQGEIMSGEGDINTIGEISMNRSLI
                     EARKDKDVKAIVLRINSPGGNALTSDLIWREIELTKKVKPVVVSMGNYAASGGYYIAC
                     NANKIFAENNTITGSIGVFGILPNFTQLATKIGIHTEQVKTNENASEYSPFVPMDEKF
                     KAVTLESVEHIYKTFVSHVAKGRKMTIAQVDSIGQGRVWSGSQALKIGLVDKIGGLDA
                     AIKEAALLGKTKKYSTQNFPEFEKDMSDLLKNFPFAKSKESFIKEELGEETYRLMEQI
                     KKVQARKGVQAMMPFEINIQ"
     gene            complement(6824..7618)
                     /locus_tag="PLQ70_00515"
     CDS             complement(6824..7618)
                     /locus_tag="PLQ70_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HQE33127.1"
                     /translation="MTYEGTLTKMQTEFANPIQYYLVFENSFLNVNQLLNKNLEINFV
                     GYQCLNCGKKKKIYRQGFCYDCFYSSPAVGDWIMKPELSTAHLGIQDRDLVYEEKVQL
                     QPHIVYLALSSEVKVGVTRKTQMPTRWIDQGATQAISIVEVPNRYLAGITEVALKNHF
                     ADKTNWQKMLKNDVVQVDLIAEKIKVQNWIPAEVQEYFYSEKNDLYEMHFPVLEYPKK
                     INSLNLDKTPNFQGKLTGIKGQYLIFEDGTVFNVRGSEGYEVRIEI"
     gene            7714..9246
                     /locus_tag="PLQ70_00520"
     CDS             7714..9246
                     /locus_tag="PLQ70_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HQE33128.1"
                     /translation="MPIAVINSFASWVLKQRIHQIELFLKYPNEVQEELLMNLIKAAE
                     KTVIGQQYDYESINSYAAFAERVPVSTYEELQPLIERTRQGEQNVLWETPIKWFAKSS
                     GTTNAKSKFIPVSPEALEDCHYKAGKDLLCMYLNNNENSEMFLGKSLRLGGSAQIYEN
                     NNTSFGDLSAILIENMPLWADFSSTPNKDISLMSDWETKIPAIINEVKNENVTSFAGV
                     PSWLLVLMNKLLEETGKGNLLELWPNLEVYFHGGVNFEPYREQYKKILPKKDFKYYEI
                     YNASEGFFAIQDLNYSSDLLLMLDYGIFYEFIPMDTFGTPNQKTIRLSDVELFKNYAI
                     VITTNSGLWRYMIGDTVRFTSLSPYKIRVTGRTKHHINVFGEELMVENTDQAIAKACQ
                     ITGAEMVDYTVAPVFMKDKEKGAHEWIIEFKKNPIDIETFQKVLDETLQSLNSDYEAK
                     RHNNMTLNPLILNIARPNLFYDWLKEKGKLGGQHKIPRLSNERDYLEQLKNMQTSYNC
                     SI"
     gene            complement(9296..9859)
                     /gene="gldD"
                     /locus_tag="PLQ70_00525"
     CDS             complement(9296..9859)
                     /gene="gldD"
                     /locus_tag="PLQ70_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HQE33129.1"
                     /translation="MSKNQLSIFALLMVLFSIFSCKKEVLPKPASYLRLDYPEANYAN
                     FESKCPFTFEMNSDAIIKGEKDCGFTITYPKMKATIYLTYKPVNNDINKLLHDAQKLT
                     YEHVIKADDILEQPFLNPTKKVYGMFYQVDGNAATNSQFYVTDSVKHFVTGSVYFYAK
                     PNYDSIMPAASFVRNDMQRLMETLKWK"
     gene            complement(9867..11162)
                     /locus_tag="PLQ70_00530"
     CDS             complement(9867..11162)
                     /locus_tag="PLQ70_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HQE33130.1"
                     /translation="MDPEPSFLFTYTLDANLVFGFIAIFALLFCSALVSGAEVALFSL
                     SQKDIDDTLQEHSSKGKIISKLLEKPKKLLATLLVANNFIKIGVVILFSFMGQNLFSA
                     IQSPLLKLGIELIIVAFLILLFGEVLPKVYASRNNIKFAKWVAYPILGLDTLLSPISL
                     PMRRATIYLHNKLGKQKTNFSVDQLSQALELTDTEETSSEEQKILEGIVSFGNTDTKQ
                     VMSPRMDIFALEIEESFAAICPKIIEMGFSRIPVYRDNIDQIEGVLFVKDLLPHLDKE
                     EFDWKTLLRKPFFVPENKKIDNLLKDFQGMKSHLAIVVDEYGGTSGLVSLEDIIEEIV
                     GDISDEFDGVNANYTKIDDKNYLFEGKTNLKDFYRVVEVDEQIFESTKGEAETLAGFI
                     LEILGNFPKKDQKIAFENCMFTIETVDKKRVKQIKVTVE"
     gene            complement(11175..11609)
                     /locus_tag="PLQ70_00535"
     CDS             complement(11175..11609)
                     /locus_tag="PLQ70_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQE33131.1"
                     /translation="MNSTLNKVMLIGHLGDDVKMHYFKADSCIGKFSLATNETYINKE
                     TGEKITSTEWHNIVVRNKAAELCEKYLSKGDKIYVEGRIKSRQWQAEDGSTKYITEIQ
                     AIDFTFLTTKKENKSIDSPKETNFDSKNNGLPENDLPSQLSN"
     gene            complement(11648..12691)
                     /gene="mutY"
                     /locus_tag="PLQ70_00540"
     CDS             complement(11648..12691)
                     /gene="mutY"
                     /locus_tag="PLQ70_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HQE33132.1"
                     /translation="MIFSNILIRWYLENKRDLPWRKTDNPYLIWLSEIMLQQTRIAQG
                     TPYFLSFVSNFPTVFDLANANEEQVLKLWQGLGYYSRARNLHKTAQFVATELSGKFPD
                     NYNDLLKLKGVGEYTAAAIASFSYNEVVPVVDGNVFRVLSRYFDVETDIAAASAKKEF
                     AALAFELMPKDNPAIFNQAIMEFGALQCVPKSPNCGVCVFNSSCAALQKKKVDQLPVK
                     SKKLKVRNRFFNYLVVADDDENTIIQKRTAKGIWHNLYEFPLIETDAPEDFDFISKTI
                     QDSFFKNNPLISVEEDSEKNIIHKLSHQHLHIQFWKVRVAGIIENGIDAVTLKTFPFP
                     IVIHNFIEKDFLS"
     gene            12825..13115
                     /locus_tag="PLQ70_00545"
     CDS             12825..13115
                     /locus_tag="PLQ70_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQE33133.1"
                     /translation="MTKADIVAKIADKLGLEKGDVQATVETFMTEVKTSLEEGDNVYL
                     RGFGSFIVKTRAEKTGRNISKNTTIKIPAHNIPAFKPAKVFVEGVKTNNEAK"
     gene            13499..15043
                     /locus_tag="PLQ70_00550"
     CDS             13499..15043
                     /locus_tag="PLQ70_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HQE33134.1"
                     /translation="MNKELIIRSSSEAVDFALLKDGKLIELHKEQETSNFQVGDIFIA
                     KIRKPVAGLNAAFVNVGYEKDAFLHYHDLGPNLASQLKFIKLVSAGKIKDFSLKNFQF
                     EKEIDKDGSITDIINSNQSILVQVVKEPISTKGPRISAELSLAGRFIVLVPFSDRVSI
                     SQKIEDKKEKERLKRLVQAVKPKGFGVIVRTVAEGKSTVELEKDLQNLIERWTAMCKK
                     LPTAHHPSKVLGELNRASSILRDVFNDTFSGVLIDDEELYNQTKDYLQEIAPSKTSIV
                     KFYQSKDTPIFEKYNIERQIKTSFGKTVSMSKGAYLIIEHTEALHVIDVNSGNRSNKA
                     SNQEDTAMEVNMIAAAEIARQLRLRDMGGIIVIDFIDMSNPENRKVLFDFLREEMSDD
                     KAKHKILPPSKFGLVQITRQRVRPEVNIKTREEDPNNGNGEIEAPIQIIDKIALDLER
                     HLKTHNKVVLNVHPFVAAYLSQGFPSLRSKWFFEHKKWVKIIPRDAYTYLEYHFFDKK
                     GNVISE"
     gene            complement(15228..17603)
                     /gene="pbpC"
                     /locus_tag="PLQ70_00555"
     CDS             complement(15228..17603)
                     /gene="pbpC"
                     /locus_tag="PLQ70_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HQE33135.1"
                     /translation="MKKKLKAFLQRIINWIKKNKIKSSMAFLLLVVYYFSLPRTLFQE
                     PYSTVIESREGELLGAKIARDGQWRFPAQDSVPDKFKKCIVYFEDQYFYKHPGFNPMA
                     MINAVKQNNRAGKVVRGGSTLTQQVIRLSRKGKGRTYFEKIIEIILATRLELRDSKEE
                     ILDLYAAHAPFGGNVVGLEMASWRYFGVQSHQLSWAESATLAVLPNAPSLIYPGKNQQ
                     KLFRKRNNLLLKLHHEGIIDSQTYELSIDEPLPQKPFDLPQIAPHLLQRVVKNQEGTR
                     VKTTVDLALQNRVNQMAKQYYNQYKQNEVNNLAILVIDIANRNVISYVGNAPTDRNHQ
                     KDVDIISAPRSTGSILKPFLYAAMLDDGELLPNTLVADIPTQISGYTPQNFNLTFDGA
                     VPAHRALSRSLNIPSVLMLQNFGVNKFYEQLQKFKLSDISKPPDHYGLSLILGGAESN
                     LWDLCRTYAGLSSTIDFFAKNQGQYRTKEFAELNYQNDFKTDFGTESFDKTILGAGSI
                     WLTYNAMEEVNRPEGDEAWKFYDSSQKIAWKTGTSFGNRDAWAIGTNSRYVVGVWVGN
                     ASGEGRPSLTGVTSAAPILFDVFNLLPRQKWFKTPLNDLEEVEVCTLSGHLAQDDCPK
                     MKQLVSLKGRTTSVCPYHKTVHLDKTVQYRVNSSCENIENIVTKKWFVLPPVMEWYYK
                     NQHIEYQPLPPFRADCASTQMPSMDFIYPKTNGKIYLTKNFNSEIQPVILKVAHSSRG
                     VQLFWYVDNIYKGSTKTFHEMPIMPSSGIHYITVEDEFGNEIRRKIEVLWE"
     gene            complement(17769..23333)
                     /locus_tag="PLQ70_00560"
     CDS             complement(17769..23333)
                     /locus_tag="PLQ70_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HQE33136.1"
                     /translation="MKAKGLVYVFFVFFVFQACSKKSSDFNSDFSLFKEYINSFTGGV
                     VSTTSDIRVVLAFDKKEWKANQVLDSDLFDISPNVDGKVVALSNNTIAFIPEEKLKSD
                     TEYQITLNLDKLISVKKELSEFRFTIKTIKQDFAVETHDVQSYNKDFQYLNGTLKTAD
                     NIDLETAKQLISAEQKGKNLKIKFDKTVSSGTEFKFIIDSIQRFPEESKVEISYDGSD
                     FNIDQKGSVDYVIAAKDVFKVMKVQIPEGNVQSLYINFSDPLQNGQDFSGLVAIENTN
                     NLKFVVQGNLLKVFFNNDGSDKLNEEVDEYGNRKPSKEINGVLLVEAFQGIENDEGQK
                     MATNFATKVSFEQTKPSVRFVKNGTILPSSNNLKLNFEAVNLSAVDVKVYKIYKSNIL
                     QFLQDNELNGTQYLKRVAQPIAKTTLNLKENKLLNFDRWNTFGLDLSKIITPEPGAIY
                     RVEFSFKKAYSLYKCESPSVDENQEEEDVVDENDVNYSGGNYYDDDYYDDDYNWQDRD
                     NPCTKSYYRNTKIGTNILASDLGVIAKRGENKSYFVAVNNIVTTEPVSNATVDLYSYQ
                     QQKLATVATDSEGIANFQLDRFAYFAIVTLGDQSTYVKLDDGNSLSVSNFDVAGETLQ
                     KGLKGYIYGERGVWRPGDKLYLSFILNDAENKLPSSHPIKFRLSDPNGKVVCQTVQRS
                     NEWNHYLFVVPTNSDAPTGNWEAMANVGGAKFYKSIKIETIKPNRLKIKNSFKNAMLS
                     SSYPNTSNLEVAWLHGAVAKNLKVEMQAKFTQQTTTFKNYSNYIFDDMVRKFSTEEIN
                     IFSGKLDANGRASVNIQPKLQGQAPGMLKASFITKVYEEGGDFSTDVMATTYSPYKTY
                     VGIKSPELNKYEMLETRKNNRFEIITVDENGYPKAVKNLEIKIYKVEWHWWWDASSDN
                     LSNYNSSNATTAYKSFQLNTGANGRGILQFALNDEEWGRYLIRVSDENEGHATALAVN
                     IDWPIWSGKTRNTDASTANMLVFSTDKKNYAVGEKAQISFPSSEGGRALISIENGSKV
                     MQTLWAKTQQGETKVEVPITAAMAPNVYLNITLLQPHASTKNDLPIRMYGIIPIEVID
                     KNTILEPKLAMPDVLRPEQQFNIKVSEKTGKAMTYTIAIVDEGLLDLTRFKTPNAWDA
                     FYVREALGVKTWDVYDEVIGAYGGKVNQIFSIGGDQDLGGGKAKKANRFKPVVVYYGP
                     FKLEKGQSKTHQIKLPKYIGSVRTMVVAADANSSAYGSVEKATPVRSPLMVLASLPRK
                     ISPSEKVTIPVTVFTMEKNIKNVTVQIKTNNGLKVVGSAVQNLTFAQPDEKMVYFNLE
                     VGNATGIGKVQIIASSGAAKSTYDVEMDMTNPNPVTSTFTDVVLPPNSSKTIAWKTFG
                     VAGSNKAKLEVSSMPTIDFNRRLQYLIEYPHGCVEQTTSAVFPQLFLNDVVDIDANRK
                     DLIQKNVTAGITRLGSFQLSNGGFSYWQGEPTADDWGSSYAGHFLIEAEKKGYVLPIN
                     FKSKWISYQQKQAKQWRFVAQYGNDLAQAYRLYTLALAGSPDLSSMNRLRETAGISNE
                     SKLRLASAYVLAGQKAAGLTLLAKSKIDESDARYNYYYYGSGDRNRAMTLETLILLGQ
                     KQNAFSMASKLAKQMSSSEWMSTQTTAYCLYSMSKFARSNGTKGIDVQFNNNGKNQVV
                     KTMKSIAERTLVVQSGANGITIKNNKNNTVFVRVLNTGILPIGQEQIIQNNLFASVVF
                     KNRKGAIINVSKINQGTEFVAVVTVKNKKNERVENVALSQILPSGFEIVNTRFTDYGD
                     ATSNIANYIDIRDDRTNFYFGLNANETKTFRILLNASYLGNYYLPGLQCEAMYDHTFL
                     ARTKGFWVQVVK"
     gene            complement(23795..23941)
                     /locus_tag="PLQ70_00565"
     CDS             complement(23795..23941)
                     /locus_tag="PLQ70_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33137.1"
                     /translation="MSKLIYFKRYLYIIDRLRSRPCSFMELQEYVIRKLENEDIYTDF
                     DLLP"
     gene            24098..25060
                     /locus_tag="PLQ70_00570"
     CDS             24098..25060
                     /locus_tag="PLQ70_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HQE33138.1"
                     /translation="MNKTFHSLLTLLFLAFFTNCYSQNPLNFGSNILTADPSGHVWKD
                     GKMYVYTSHDEECQKDFYMKDWHVFSSSDLVNWTDHGACLSVNDLSWADNFAWAPDCG
                     YKNGKYYFCFPAGNGFKDRVNSKNSTKWMGIGIAVSDSPTGPFKDAIGKPLWTKPYAN
                     DPCLFIDDDGKGYVYFNGGGDYLVAKLADDLLSVKGDIQPMNMGGYKPKREGPWVFKR
                     KDTYYYTMPENNRILTYYTSKTPIGPWKYQGVIMDSEGESNNHHSIVEFKGKWVLFYH
                     RWVENKSECGEKQRKVCAENLYFNDDGTIQKVSRTDAGIGNLFR"
     gene            complement(25107..25550)
                     /locus_tag="PLQ70_00575"
     CDS             complement(25107..25550)
                     /locus_tag="PLQ70_00575"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HQE33139.1"
                     /translation="MENPFTNDYFMKKALQEAEMAFEKGEIPVGAIIVVADKVIARSH
                     NLTELLNDVTAHAEMQAITAAANFLGGKYLKDCTLYVTLEPCQMCAGALYWSQISKIV
                     FGARDEQRGFMTMGTQLHPKTTVVSGVMANEAADLMKRFFATKRK"
     gene            25589..27358
                     /locus_tag="PLQ70_00580"
     CDS             25589..27358
                     /locus_tag="PLQ70_00580"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQE33140.1"
                     /translation="MTKNLLQHINNPSDLRQLDEAQLPQLAQELRDFIIGVVAVKEGH
                     LGASLGVVELTIGLHYVFDTPNDLLIWDVGHQAYGHKILTERREIFHTNRQLNGISGF
                     PKRSESVYDAFGVGHSSTSISAALGMAIGSNLKGDFEKQHIAVIGDASIASGMAFEGL
                     NHAGVTDANLLVILNDNAIGIDPSVGALKKYLTAVKEGKNPKKNNMIKSLNFDYSGPI
                     DGNDIFAVLKELKRLQKIKGPKFLHLITTKGKGLQQAEEDQVKYHAPGKFDASTGEIH
                     PKQEENLPPKYQDVFGLTLLDLAKANEKIIGITPAMPSGSSLKFMMDEIPERAFDVGI
                     AEQHAVTLSAGMATQGMVVFCNIYSTFLQRAYDQVIHDVALQNLPVIFCLDRAGLVGE
                     DGATHHGVFDLAYLRCIPNMIVYSPLNEIALRNILYTAQLGLNHPIAIRYPRGRGQIV
                     DWQKPYEKIEIGKANCLKEGTKTAILSNGTIGNNVTLALAEINHPENFAHYDFAFVKP
                     LDEKLLHTIFKSFKNIITVEDGVVKGGFGTTIVEFASNNNYNSSIRTLGIPDEFIEQG
                     TVYELQHYCKIDVSGLKTVFMNY"
     gene            27397..28500
                     /locus_tag="PLQ70_00585"
     CDS             27397..28500
                     /locus_tag="PLQ70_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HQE33141.1"
                     /translation="MKLYSFSLLLLILFVPMIGFSQKSNLHSLKPVIYGDSLSVGVVQ
                     DSIKPIVALDVAITPIIKYTSPPKSKMAVAKKIDIPFISMSHWINKNSVGIDLTQIAF
                     VNWNAGGTSSVAGLFTGTFTRTRSDINSEWINELIVKYGLNKQDGLDMRKTDDDFRFN
                     STFGYRQDTFSNWYFSAKFNFRTQFTDGYKYPDTKNPISKTFAPAYTFLGAGADYFNK
                     EKKFDVYISPLTLKNTLVLDQTLADKGAFGMIKATYDTEGNKLTSGEKSRTEVGILVT
                     NYYKKEVWKNITWENRLSLYTDYINNFGNIDVDWKMQLDLIVNQHVKANIGSQLIYDD
                     DVKNIEKIDGVQVVSGAKVQLKQSLGIGMVYNF"
BASE COUNT         9232 a         5475 c         5045 g         8927 t
ORIGIN      
        1 gcaggattta ttagcattgg ctaatccgct tttccatccc acaattacta catcttggag
       61 tgcatcagaa ggaaaactaa aactgtatag cccaaccgga attccggtta gttattcaga
      121 ttttgaagcc gcaataaaag atgtaacctt tactaattcg tccgcatcac catctggaac
      181 tcgtgatttt tctattaatt tgggtttcgg ttctgctaat tatttaccct caaacggaca
      241 tttctatgaa tatgtggcaa gtccaggtat tatttggaca gctgcaaaag atgcagccgc
      301 cgcaagaact tattatggct tgcagggtta tttagctaca ttaacagctg ctgacgaagc
      361 acaattggct ggagcacaag cgccaggaac gggatggatt ggcggtagtg acgcacaaat
      421 tgaaggtact tggaaatggg taactggacc agaagggctt gccaatggag gaactggaat
      481 tactttttgg actggaaaag gcaatggcac aacaactcct ccttttaatt atgccaattg
      541 gaacgctcct aatgaaccta atgactctgg aaataacgaa gattatgccc atataacttc
      601 tccagcagtt ggaaacctag gaacctggaa tgatttgcca gtacaaggca atgctataaa
      661 tgtagccaat ggtaattact tccctcaagg ttatattgta gaatatggag gaatgcctgg
      721 cgagccaaca cttcaaattt cggcgagtac taaaatcact attccaaaaa taacaagttc
      781 aactccaagt gctatctgtg gttctggaag tctgatacta caagcaactg caactgatgg
      841 cacagtaaat tggtacacaa ccccaactgg aggaacatca atttatacag gaaattcatt
      901 tacaactcca gttctaacta ctaccacttc ttattatgta gatgcatcaa atggaagttg
      961 cccaaatggt cctagaactg aaattattgc tactataaaa cctttaccca atttgccaca
     1021 aatcgcaatt tcttcgacaa gtccggtttt atattgtttg aatgctacag caatcccatt
     1081 agcagcaaca gcagacagta actgcaaatt aaattggtac acccaagcag ttggaggtat
     1141 tgcttcagct acaagtccaa caccaattac aacaacagca ggaacgacaa actattatgt
     1201 gagtcaaacc aatactttaa gcaactgcga aagccttcgt gctgcaatca tagtaacagt
     1261 caatccattg ccaacggttc ccactgtaaa caatatttcc tattgcaata atgaaactgc
     1321 tgttcctttg acagctattg ctacagcaaa ttgcacttta aattggtatc caactgctac
     1381 aggaggcacg ccgagtgcaa caagtccaac gccttctaca atagtagttg gtacaacatc
     1441 ttattttgtc agtcaaacca taacttcaac gggttgtgaa ggacctagag ccgaaatagt
     1501 agtcaccgtt aacccattgc ctacagcacc cattgtaagt aatattgctt attgcaatct
     1561 tgaaacggca attccgctaa cggctacagc atccccaaat tgtactttaa attggtatgc
     1621 agcttcagta ggtggaactt ctagcgcaac aagccccacg ccatctacag caactattgg
     1681 aactacaaaa ttttatgtaa gtcaaaccat tgtggctact ggatgcgaaa gtccaagatc
     1741 ggaaatttca gtcattataa atccattgcc gattgtaaac gatgtcacta ttattcaatg
     1801 cgatgccgac ttagttgttg acggtaagac gctatttaat ttgaccgtaa acaatgctca
     1861 aatttcggca aattatacca atgaaacgtt tacttattac acctctttaa acggagcaaa
     1921 aaatgctatt ccagccgatt tgattccaaa tgaattggct tttcaaaaca caactccaac
     1981 acaaatggac gtttggtcgc gaattaccaa taaaacaaca ggctgtttta gtgttgccaa
     2041 aataaccttg aaagttcctg ctacgaatat caatccgaat acaaaaattc cttttcctgc
     2101 tgtttgtgat gattttttag atgccaacgg aattaataac gccaataaca acaatcgaga
     2161 tggaattaca acttttgatt tttcttcgac aaaagcaata atacaagccc ttttgcctcc
     2221 aaccgatgtt tacaacatta attattatcg aaaccaagcc gatgctttgg ccgaactcaa
     2281 tgttatctct gacatttcaa attatcgaaa cattggttac cctaattctc aggatatttg
     2341 ggtacgaatt gatagtaatc tggacaatgc ttgttacggt ttaggacctt atttgacttt
     2401 aaatgttgaa gctttgccaa tcgcaaatac agtaaccatt ccgagacaat gtgacgacaa
     2461 taatgatgga atatttactt tcaatacttt aaatcttgag tcaactctat taaatggaca
     2521 atctaacaaa acggttactt atttcgacca agccaataat cccctgaaag atgccaacgg
     2581 aattttgatt aaaagcccat tcccagccac tttcacttca acatcccaaa cgattaaagc
     2641 cgttgtgacc aataacagtc cgcaacattg ttatgacgaa acaaacattc tatttattgt
     2701 tgaccaatcg ccagtagcat ttgcagtccc ttcaaatttg acaacaactt gtgatgacga
     2761 acccaatcca atagaccaag atggaaaatt tgcttttgac accgctactt tcgaaagcac
     2821 aattcttggc ggacaaactg gaatgattgt caaatattac gaccaaaaca atatgctttt
     2881 gccaagtccg ttgccgaatc catttgtaac caacacacaa aacattacag cacaagttca
     2941 aaatccatta aacacaactt gcactgcatc aacaacttta aacttcatag tcaatcctgt
     3001 cccaaatatc gacttgaatc tcgatggtag tgccaatgaa ttagtatgtc agaatgattc
     3061 ggcctttttt gtaaccttag atgcaggtat aacagacggt tcgtcaacta caaattatga
     3121 ttatacttgg acaaaagatg gagttcatac cgggacaaac tcaccaacat tagacgtaaa
     3181 tgccgaagga gtttataccg ttgaagtcac caatcattcg ggttgcagtc gaattcgaac
     3241 attgaccgtt acagcatcaa atattgcatc gattacttca gttgatgttg tcgatttgac
     3301 cgaagtgaat acaataacag ttaatgtcac tgggccagga gtatatgaat acagtttaga
     3361 tgaacccaat gggttctggc aggattccaa tttttttaat aatgttcccg ctggagttca
     3421 cgtcgtttat gtgaatgata aaaatggctg tggttccgtt catcaagaga ttgtacttgt
     3481 aggtgttcct aaatttttca caccaaacgg tgatggttat aatgattatt ggagtataaa
     3541 aggagtgaat gaaacctata attcaaattc cataatctac atttttgacc gatacggaaa
     3601 actcctaaaa caatgggttc cttctctaaa tcaaggttgg gatggcactt tcaacggcgt
     3661 tcctttacct gccgatgatt attggtttac tctaaaactg gaagacggtc gagaaacaaa
     3721 aggacatttt tctttaaaaa gataattttc taaattagtt actaattttt cgccgaattt
     3781 cgtccaaaat ataaagataa tcttcctggt ttttcacaaa atcacggtct gaaacatcga
     3841 taatcaagac attcaaattc gtttgcgttt tgatgtaatc cagataaccg ccattgattt
     3901 tatctaaata atcggcagga atattttgct cgtaacttcg gcctcttttc ttgatatttt
     3961 ccagcaaacg ctccgtattt tggtataaat aaacatacaa atccggtttt ggcatttccc
     4021 gatacacaat atcaaacatc gttttataca atcggaattc gtcttcctga agcgttactt
     4081 tggcaaaaat cagggatttg aaaatatgat aatcggctac tataaaatcc ttgaacaagt
     4141 cgaattgggc caaatcatcc gacaattgtt tgtatctatc ggcaagaaac gacatttcca
     4201 acggaaaagc ataacggttt tgatcttcat aaaatttagg aagaaaggga ttgtcggcaa
     4261 agcgttctaa aacagttttg gcattaaaat cctccgcaat tttggtagcc aatgttgttt
     4321 ttccagctcc aatattgcct tcaaaagcga tataattgaa ttgttccaaa ggaattttgt
     4381 ccaatgggtt ttttaaactt tgaactagga cacaaacact ttcgtcagga gaaatttcga
     4441 ataattcagc cgttttttta tgcaaaacag gatgaatcca attcaaattc aaatccaaca
     4501 tgggcaataa aacaaaattt ctattttgca ataaaggatg tgggacttga agatcttcac
     4561 tatcaataat ttcttcgtta aaagtgatta aatcaatatc aataatccgt gattggtatt
     4621 cttgagtcgt atttcgaatg cgtcctaatt gtttttcaat gcttaaaatt tttgtcaaaa
     4681 ttgcattcgc tgacaaaaaa gtgtgcaaaa ccaaagcgca attataaaaa gcatcgctat
     4741 caaaacccca agacggagtt tcatacaact ttgatgtttt gataacagtt ccaacttctt
     4801 gatgaatcaa ttcaatagat ttttcaatgt tttctagacg attgccttga ttggttccta
     4861 gcgataaaac gacctgattt tgtgatttca taataagcac aaattaacta aaacaatttt
     4921 agaattgaac tatatttgcc tcgctgttga taacttaaat ttgtaacata tcaactcttt
     4981 ttgctactta taaaaaaact attgttatga gatttttagg aaatgttttg gcaaccatag
     5041 tggggttgtt cgtttttata atgttattcg tttttggaat tgtattaatc ggaatgttat
     5101 ttggtggcga atcggatgaa atttctgtaa aaagcaattc ggttatcgaa ttaaaattag
     5161 acgaaataaa aaacgattat gctggaaaat acaacaaccc ttgggttacg gaattttcta
     5221 aaggaggaaa agtaggacta tcagacgtta tcaaagctat tgaagccgct aaaaccgatt
     5281 cagacatcaa aggaatttct attttgaatg atgaatctga actgggaatg gcacaaagaa
     5341 aagctttgcg caacgcctta gaagacttca agaaatcagg aaaatttgtg atggcttatg
     5401 ccaattccta ttcacaaaaa gaatattatt tgaattctgt tgccaataca atttacatca
     5461 atcccgttgg tgatttcgat ttcaaaggat tatctgccga attgatgttt ttcaaagatc
     5521 ttcaagaaaa atcgggtatt aagatggaag tcattcgaca cggaaaatac aaaagcgccg
     5581 tggagccttt cttagaaaat ggaatgagcg atgccaatag agaacaaacc acatcattac
     5641 taaacgcggt ttggagttct gttttagctg atatttccaa aagcagaaat atatcggtag
     5701 ccaaattaaa cgaaatcgcc tctggtcttc ttgctcgaac tccagaaatg gcaaagtccg
     5761 aaaaattagt tgatgtcatt gcttatgaag atgagtatca cgatgccatc aagaaaattc
     5821 tgaaagttga caaagatgaa gattacaaca aaacagccat tttagactat gctcaaaaag
     5881 tagccactac ttctgaaagc aatgattcta atgataaaat tgccattatt tatgcgcaag
     5941 gagaaattat gagtggcgaa ggcgacatca acaccattgg cgaaatctcg atgaatcgct
     6001 ccttgattga agcccgaaaa gacaaagatg taaaagctat tgtacttaga attaatagtc
     6061 ctggcggaaa tgccttgaca tcggatttga tttggagaga aattgaatta accaaaaaag
     6121 tgaagcccgt agtggtttcg atggggaatt atgccgcttc tggtggttat tatattgctt
     6181 gcaatgccaa caaaattttt gccgaaaaca acaccattac aggttcgatt ggcgtttttg
     6241 gaatcttacc aaatttcaca caattggcaa ccaaaattgg aatccacact gaacaagtaa
     6301 aaaccaacga aaacgcttct gaatacagtc cgtttgttcc gatggatgag aaattcaaag
     6361 ccgtaactct tgaaagtgtg gagcacattt acaaaacatt tgtgtcacac gttgcaaaag
     6421 gacgcaaaat gacgattgct caggtagatt caatcggaca aggaagagtt tggtcgggct
     6481 cacaagcttt aaaaattggg cttgtagata aaattggagg tttagatgcc gctattaaag
     6541 aagccgcact ccttggcaaa accaaaaaat acagcactca gaactttcct gaattcgaaa
     6601 aagacatgag tgatcttttg aaaaattttc cttttgcaaa atctaaagaa tcgttcatca
     6661 aagaagaatt gggtgaagaa acctatcgtt tgatggaaca aattaaaaaa gtgcaagctc
     6721 gaaaaggcgt tcaagctatg atgccttttg aaatcaatat tcaataattt tttttaatag
     6781 ttgacccttt tagtcctatg ccattggatt gaaagggttt gttttatatt tcaatccgta
     6841 cttcataccc ttctgaacct cgaacattaa aaacggtgcc atcttcaaaa atgagatatt
     6901 gtccttttat tccagtcaat ttcccttgaa aatttggggt tttatccaag ttcaaactgt
     6961 ttattttttt gggatattct aggacaggaa agtgcatttc atacaaatca tttttctctg
     7021 agtagaagta ttcctgaact tcggctggaa tccaattttg aactttgatt ttttcggcga
     7081 ttaaatctac ttgcacaaca tcatttttaa gcattttttg ccaattggtt ttatcggcaa
     7141 aatgattttt caaggctact tcagtaattc ctgccaaata acgattgggc acttctacaa
     7201 tcgaaatggc ttgggttgcg ccttggtcaa tccatcgagt gggcatttgc gtttttctag
     7261 tcacgcctac ttttacttcg cttgacaaag ctaaatagac aatatgaggt tgcaattgta
     7321 ctttttcttc ataaaccaaa tctcgatctt gaatgccaag atgtgcggta cttaattcgg
     7381 gtttcataat ccaatcgcca acggctggac tcgaataaaa acaatcatag cagaatcctt
     7441 gtcggtagat tttcttcttt ttaccacaat tcaaacattg ataccccaca aaattgattt
     7501 ccaaattctt gttcagtaat tgatttacat tcaaaaaact attctcaaaa accaaataat
     7561 attgaatagg attggcaaat tcggtttgca tttttgtaag cgtgccttcg taagtcattt
     7621 tatctaaagt ttcagattta aaggttcaag ttgaacaaat tttgttattt ttggtaaagt
     7681 tatcactcct aattcataat tcataattta aaaatgccaa tagctgtaat caattcgttt
     7741 gcttcttggg ttctcaaaca aagaattcat caaatagaac ttttcctgaa gtacccgaat
     7801 gaagttcagg aagaattgct aatgaatttg attaaagctg ccgaaaaaac ggttataggc
     7861 cagcaatatg attatgaatc tataaattca tatgctgctt ttgccgaaag agttcctgtt
     7921 tcgacctacg aagaattaca acccttgata gaaagaaccc gacaaggcga acaaaatgtt
     7981 ttgtgggaaa ctccgataaa atggtttgcc aaatccagcg gaacgaccaa tgccaaaagc
     8041 aaatttattc ctgtaagtcc tgaagcattg gaagattgcc attacaaagc cggaaaagat
     8101 ttgttatgta tgtacttgaa caataacgaa aattcggaaa tgtttttagg aaaaagtctt
     8161 cgtcttggtg gaagtgctca aatctatgaa aacaacaata cttcgtttgg cgatttatcg
     8221 gccattttga ttgaaaacat gccactttgg gctgatttta gcagtacacc aaataaagat
     8281 atttcattga tgagcgattg ggaaactaaa attcctgcca taatcaacga agttaaaaac
     8341 gaaaatgtaa ccagttttgc cggagttcct tcctggcttt tggttttgat gaataaatta
     8401 ttagaagaaa ctggcaaagg aaatttactc gaactttggc ccaatctcga agtctatttt
     8461 cacggtggcg taaactttga accctatcga gaacaataca aaaaaatatt acctaaaaaa
     8521 gatttcaaat attacgaaat atacaacgca tccgaaggat tttttgccat tcaggattta
     8581 aattattcta gcgatttatt gctgatgttg gattatggga ttttttatga atttattcca
     8641 atggacactt ttggaacacc caatcaaaaa acaattcgat tatcggatgt agaacttttt
     8701 aaaaattatg ccattgtcat cacgacaaat tctggtttat ggcgttatat gattggcgac
     8761 accgttcgat ttacttcctt gagtccctac aaaattcgag taacaggaag aaccaaacac
     8821 catattaatg tttttggcga agaactgatg gtagaaaata cggatcaagc cattgccaaa
     8881 gcttgccaaa ttactggtgc tgaaatggta gattataccg ttgctcccgt ttttatgaaa
     8941 gacaaagaaa aaggggctca cgaatggatc attgagttca agaaaaaccc tattgatatt
     9001 gaaactttcc aaaaagtatt ggacgaaaca ttgcagtctt tgaattctga ttacgaagcc
     9061 aaacggcaca acaatatgac tttaaatcct ctgatattaa acatagcaag acctaattta
     9121 ttctacgatt ggctgaaaga gaaaggaaag ttaggcggtc aacacaaaat tccgagattg
     9181 tctaatgaaa gagattattt agaacaattg aaaaatatgc aaacctctta caattgttct
     9241 atttaaggaa tacaaattca ttaagaaaag ttaaatattt cttcgcggtt aaatctcact
     9301 tccacttcaa agtttccatc aacctttgca tatcgtttcg gacgaagctt gctgcgggca
     9361 taatggaatc gtaattgggt tttgcataga aataaactga gcctgttaca aaatgtttga
     9421 cactgtcggt cacataaaac tgtgaattgg ttgccgcatt tccatctact tgataaaaca
     9481 ttccatacac tttcttggtc ggattcaaaa agggttgctc tagaatatca tcggctttga
     9541 tgacgtgttc ataggttagt ttttgagcgt cgtgtaacaa tttattgatg tcgttattta
     9601 cgggtttgta cgtcaaataa atcgtggctt tcatttttgg atacgtaatc gtaaatccac
     9661 aatctttctc tccttttatg attgcatccg aattcatttc gaatgtaaaa gggcatttgc
     9721 tttcaaaatt agcatagttg gcctcgggat aatccaaacg caaatagctt gcaggcttgg
     9781 gcaaaacttc ttttttacaa ctaaaaatag aaaatagcac catcaaaagt gcaaatatag
     9841 aaagctgatt ttttgacatt ttataattat tctacggtga cttttatttg ttttactctt
     9901 ttcttatcta ccgtttcaat ggtaaacata cagttttcga aagcgatttt ttgatctttt
     9961 tttggaaaat tacctaaaat ttcgagaatg aatccggcca gtgtttcggc ttctcctttt
    10021 gttgattcga atatttgttc atcaacctca accactctat aaaaatcttt gagatttgtt
    10081 ttgccttcaa aaagataatt tttatcatca attttagtgt aattagcgtt aacaccatca
    10141 aattcatcgc taatatcgcc cacgatttct tcgataatat cttctaaaga aaccaatccc
    10201 gaagttccac catattcgtc caccacaatg gccaaatggc ttttcattcc ttgaaaatct
    10261 ttgagcaaat tgtctatttt tttattttcg ggtacaaaaa atggttttcg caacaatgtt
    10321 ttccaatcaa attcttcttt atcaagatga ggaagcaaat ccttcacaaa caaaacgcct
    10381 tctatttgat ctatattgtc tcgatataca ggaattctag aaaaacccat ttctataatt
    10441 ttgggacaaa tggcagcaaa agactcttcg atttctagtg caaaaatatc cattctcgga
    10501 ctcataactt gcttggtatc ggtgtttcca aaagaaacta ttccttctaa tattttttgc
    10561 tcttcagacg aggtttcctc agtatcggta agttctaatg cttgcgacaa ttgatctacc
    10621 gagaaatttg tcttttgttt tcctaattta ttatgcaaat atatagttgc tctcctcata
    10681 ggcaaactta ttggcgaaag caaagtgtca agacccaaaa tgggataagc aacccatttg
    10741 gcaaacttga tattgtttct gctggcataa actttgggca aaacttcgcc aaacaataaa
    10801 atcaaaaagg caactataat tagctcaata cccaatttta ataaaggaga ttggatagcc
    10861 gaaaataaat tttgtcccat aaatgaaaac aaaattacca ctccaatttt gataaaatta
    10921 ttagcaacaa gaagtgtagc aagcaatttt ttgggctttt ctagaagttt agaaatgatt
    10981 tttccttttg aagaatgttc ttgcaaagtg tcgtcaatat ctttttgcga caaggaaaac
    11041 agggcaactt cggcaccaga aaccagagca gaacaaaaca ataacgcaaa aattgcgata
    11101 aaaccaaata ctaaattggc atctaaggta taagtaaaaa gaaaactggg ctctgggtcc
    11161 aaattgaaaa aaaattagtt agacaattgt gacggtaagt cgttttcagg caaaccgtta
    11221 tttttcgagt caaagttagt ctcttttgga gaatcgatag atttattctc tttttttgta
    11281 gtaagaaaag taaaatctat ggcctgaatt tcggtaatat attttgtaga tccgtcttcg
    11341 gcctgccatt gacgtgattt tatgcgacct tcaacataaa ttttatctcc tttagacaag
    11401 tatttttcgc ataattctgc cgctttattt cttacaacaa tattatgcca ctcggtagaa
    11461 gtgatttttt cgcccgtttc cttattaata taggtttcgt ttgttgccaa agaaaatttg
    11521 ccaatgcaag aatcagcttt gaaatagtgc atcttaacat cgtcgcccaa atgacctatg
    11581 agcattactt tatttaaagt cgaattcata aatggtgttt tctactttca aatgtacaaa
    11641 ttttgagtta gctcaaaaaa tccttttcga taaaattatg aataacgatg ggaaaaggaa
    11701 atgtttttaa agtaacagca tcaattccgt tctctataat tcctgcaacc cgaaccttcc
    11761 aaaattggat atgcaaatgc tgatgcgaca atttatggat gatatttttc tcactatctt
    11821 cttcgacact tattaaagga ttatttttaa aaaaactgtc ttgaatcgtt ttggaaataa
    11881 aatcaaaatc ttctggagca tcagtttcaa ttagtggaaa ttcgtaaaga ttatgccaaa
    11941 ttcctttcgc agtccttttt tgaattatag tgttttcatc atcatcagca accactaaat
    12001 aattaaaaaa ccgatttcgt accttcaact tcttagattt tactggcaat tgatccactt
    12061 tcttcttttg tagagcggca caactgctat taaaaacaca aacaccacaa ttgggacttt
    12121 taggaacaca ttgcaaagca ccaaattcca ttatagcttg gttgaaaata gctggattat
    12181 ctttgggcat caattcgaaa gccaaagccg cgaactcttt tttggcagat gccgcagcaa
    12241 tatcagtttc tacatcaaaa tagcgagaca aaaccctaaa aacatttcca tcaacaacag
    12301 gaacgacttc attataggaa aaagaagcaa ttgcagcagc ggtatattcg ccaacacctt
    12361 ttagtttcaa taaatcatta tagttgtctg gaaattttcc tgataattct gtagcaacaa
    12421 actgagccgt tttgtgcaaa tttctggctc gagaataata acccaaacct tgccatagtt
    12481 ttaagacttg ttcctcattg gcattggcca aatcaaaaac tgttggaaaa ttggacacaa
    12541 aagacaagaa ataaggcgtt ccttgagcga ttcgtgtttg ctggagcata atttctgaga
    12601 gccaaatcaa ataaggatta tcggtttttc gccacggcaa atcgcgttta ttttctaaat
    12661 accaccttat cagtatgttg gaaaagatca ttgttaaatt ttagtttaca aaagtaaaag
    12721 tttatgttgt taaaatttaa tgaattagct tgaataattg ttttttttat tcctatattt
    12781 gcaacctcaa aaaattagta cacatttata aattacgaat taaaatgaca aaagcagata
    12841 tcgttgcaaa aatcgcagac aaattaggtc ttgaaaaagg agatgttcaa gcaaccgtag
    12901 agacctttat gactgaagtt aaaacttctt tagaagaagg agacaatgtt tatttaagag
    12961 gttttggaag ttttattgtg aaaacaagag ctgaaaaaac aggaagaaat atttcaaaaa
    13021 atactactat caaaattcct gctcacaaca ttccagcatt taaaccagca aaagtttttg
    13081 ttgaaggagt aaagacaaat aacgaagcaa aataaaaata ttattaatca caaaatcacc
    13141 agattatgcc aagtggtaaa aaaagaaaga gacataaggt agcaacgcac aaacgcaaaa
    13201 aaagagcgag agctaaccgt cacaaaaaga aaaagtagtt tataactact tttttctttt
    13261 taaacgttca ttgaaatttg gatttcagac ttcagatttc agacttcaga ttgcggccta
    13321 aactgcaaac tgctctctga atactgcaaa ccgacttcct cccgggttca atacctgttc
    13381 aaattattgt ttaatccatc cttgagaagt tgatggttgg tagttgattg ttgatggata
    13441 ttttccaaca accgacaacc aacagccaac aacccattca cggataaaaa tttacagagt
    13501 gaataaagaa ttaatcatta gatctagttc tgaagccgta gattttgcct tattaaaaga
    13561 tggaaaacta attgaattgc acaaagaaca agaaacaagc aactttcaag ttggcgatat
    13621 ttttattgcc aaaatcagaa aacctgttgc aggactaaat gcagcttttg taaatgtagg
    13681 ctacgaaaaa gacgcctttt tgcattatca cgatttgggt ccaaaccttg cttcccaact
    13741 gaaattcata aaacttgtaa gcgcaggtaa aataaaagat ttctccctaa aaaactttca
    13801 gtttgaaaaa gagatagaca aagatggatc gataacagat attatcaatt ccaatcagtc
    13861 tattttagta caagttgtca aagaaccaat atctaccaaa ggaccaagaa ttagcgcaga
    13921 gctttccttg gctggaagat ttattgtttt ggttccgttt tctgaccgag tttctatttc
    13981 acaaaaaata gaagacaaaa aagaaaaaga acgtttgaaa agactcgtac aagctgtcaa
    14041 accaaaagga tttggtgtta ttgttcgcac agtagccgaa ggcaaaagca cagtagaatt
    14101 agaaaaagat ttgcagaacc tcatagagag atggactgca atgtgtaaaa aattaccaac
    14161 cgctcatcat ccgtccaagg tattaggaga gctcaataga gcttcatcaa tattaagaga
    14221 cgtcttcaac gacaccttta gtggtgtcct aatagatgac gaagagttgt acaaccaaac
    14281 gaaggattat ttacaagaaa tagccccttc caaaacatcg attgttaagt tttatcaatc
    14341 caaagacact ccaatttttg agaaatacaa tatagagaga caaatcaaga cctcctttgg
    14401 aaaaacagtt tctatgagca aaggtgctta tcttattatc gaacataccg aagctttgca
    14461 cgttatagac gtgaatagcg gaaaccgttc taataaagca tctaaccaag aagatactgc
    14521 aatggaagtc aatatgattg ccgccgccga aatcgccaga caattacgtc ttcgtgatat
    14581 gggcggaata atcgtaatcg attttatcga tatgtcaaat ccagaaaatc gcaaagtcct
    14641 gttcgacttc ctaagggaag aaatgagcga tgataaagca aaacacaaaa tcttacctcc
    14701 aagtaagttt ggcttagtcc aaattactcg acaaagagta agacccgaag tcaatatcaa
    14761 aactagagaa gaagacccaa acaatggaaa cggcgaaatt gaagcgccaa ttcaaatcat
    14821 tgacaaaatt gctttagatc tagaaagaca tttaaaaacc cacaacaagg ttgtccttaa
    14881 tgtacatcca tttgtggctg cttacctcag tcaaggtttt ccatcattgc gttcaaaatg
    14941 gttttttgag cacaaaaaat gggtgaaaat cataccacgt gacgcttaca cgtacttaga
    15001 atatcatttc tttgacaaaa aaggaaatgt tatttcagaa taaaaaatga ttctgaaata
    15061 aattcagaat aaaaaaccgc ctttcgagag attggcggtt tttttgtgct ttttttctat
    15121 tttttttagg agcttaatcc agctatcagt taccatcttt tgtgccgaac accggcacaa
    15181 aaggatttca acttctatct gggctaggaa atttgttttc aaaaaaccta ctcccacaaa
    15241 acctcaatct ttctccgaat ttcgtttcca aactcgtctt caaccgtaat ataatgaatt
    15301 cccgaactgg gcattatagg catttcgtga aaagttttgg tgcttccttt ataaatattg
    15361 tccacgtacc aaaacagctg aacgcctctt gacgaatgcg ctactttcaa aatcacaggt
    15421 tgaatttcgc tattgaaatt cttagtcaaa tagattttcc cgttggtttt tggataaata
    15481 aaatccatcg aaggcatttg tgtcgaggca caatctgccc gaaaaggagg caaaggttgg
    15541 tattctatat gttggttttt ataataccat tccataaccg gtggcaagac aaaccatttt
    15601 ttggtcacga tattttcaat attttcacaa ctgctattga cccgatattg cacagttttg
    15661 tccaaatgaa ctgttttgtg gtatgggcaa accgaagtcg ttcttccttt caaggaaacc
    15721 aactgcttca ttttcgggca atcatcctgc gccaaatgac cgctcaaagt gcatacttct
    15781 acttcttcca gatcattcag cggtgttttg aaccattttt gtctgggcaa caaattaaaa
    15841 acatcaaaca aaatgggtgc tgcactggta actcctgtca aacttggtct tccttcgcca
    15901 gaggcatttc caacccaaac cccaacaaca taacgagaat tggtgccaat ggcccatgca
    15961 tcgcgattcc caaaactggt acctgttttc caggcaattt tttgcgagct atcataaaat
    16021 ttccaagctt catcgccttc aggtcgattc acttcttcca tagcattata ggtcagccaa
    16081 atagaacctg ctcctaaaat ggttttatca aaactttccg ttccaaaatc ggttttaaaa
    16141 tcattttggt aattcaattc ggcaaattcc ttggttctgt attgtccttg atttttggca
    16201 aaaaaatcaa tcgtcgaaga caaacctgca taggttcggc acaaatccca caaattactt
    16261 tcggctccac caagaatcaa agacaatccg taatgatctg gcggtttaga aatgtcgctc
    16321 aacttgaatt tttgtaattg ttcataaaac ttgtttaccc caaaattctg taacatcaaa
    16381 accgaaggaa tattcaacga ccgagataaa gcccgatgtg ccggcacagc tccgtcgaaa
    16441 gtgagattga aattctgggg cgtgtatccc gaaatttgag tcggaatgtc ggcaaccaag
    16501 gtgttgggca acaactcacc atcgtctaac attgcagcat acaaaaacgg tttcaaaata
    16561 cttccggtac ttctgggtgc actaataatg tcaacatctt tttgatgatt cctgtccgtt
    16621 ggtgcattgc ccacataact tatcacgttt ctattggcaa tatcgattac caaaattgcc
    16681 aaattattga cttcgttttg tttgtactga ttgtaatatt gttttgccat ttggttcacc
    16741 ctattttgca aagccagatc aaccgtggtt tttacccgag ttccttcctg atttttgacc
    16801 acacgctgca ataaatgagg cgcaatttgt ggcaaatcaa aaggtttttg cggcaagggt
    16861 tcgtcaatgg aaagttcgta agtttggctg tcgataatgc cttcgtgatg cagtttcaac
    16921 aaaagattgt ttcgttttcg aaataatttc tgttgatttt ttccaggata aatcaggctg
    16981 ggtgcattgg gcaataccgc caaagtcgca ctttctgccc aagacaattg atgcgattgc
    17041 accccaaaat aacgccacga agccatttcc aagcccacaa catttccgcc aaatggagcg
    17101 tgagccgcat acaaatccaa aatttcttct ttggaatctc gaagttcgag tcgtgtagcc
    17161 aaaataattt cgataatttt ttcgaaatac gttctgcctt ttccttttcg ggataaccga
    17221 atcacttgct gggtcaaggt gcttccgcca cgaaccactt ttcctgctct gttgttctgt
    17281 tttaccgcat tgatcatcgc cattggatta aaaccaggat gcttgtagaa atattggtct
    17341 tcaaaataga cgatgcattt cttgaattta tctggcacgc tgtcttgagc cggaaaccgc
    17401 cattgtccgt ctctagctat tttggcgcca agcaactccc cttccctact ttcaatcacg
    17461 gtggaataag gctcttgaaa caaggttcta ggcaacgaaa aataataaac caccaatagc
    17521 aaaaaggcca ttgatgattt aattttattc ttctttatcc aattgatgat gcgttgtaga
    17581 aacgctttta gtttcttttt caaagtcttt ttatttaacc gcaagggtcg caaagttata
    17641 cgcaaagttc actgaaaata tttaaaatta tagtcacaga ttttacggat taaacggatt
    17701 ttatatttag tgatttttta aatctgcgaa aatccgttta atctgtgtca tccgtgtacc
    17761 attaagcgct acttcaccac ctgaacccaa aaccctttcg ttctcgccaa aaacgtatgg
    17821 tcgtacatcg cttcgcattg caatcccggc aaataatagt tccctaaata agaagcattg
    17881 agcaagattc tgaatgtttt ggtttcattg gcattcaatc caaaatagaa attagttctg
    17941 tcatcacgaa tatcgatata attggcaatg ttgcttgtgg catctccata atcggtaaaa
    18001 cgcgtgttga cgatttcaaa acccgaaggc aggatttgcg acaaagccac attttctacc
    18061 cgttcgtttt tcttgttttt gacagtaacc acggccacaa attctgttcc ttgattgatt
    18121 ttagacacat tgataatggc gcctttcctg tttttgaaaa ccacactggc aaacaaatta
    18181 ttttgaataa tctgctcttg accaatagga agaattcccg tattgagcac tcgaacaaaa
    18241 accgtgttgt ttttgttgtt ttttatcgtg atgccgtttg ccccagattg tacaaccaaa
    18301 gttctttcgg caattgattt catcgttttt acaacttggt tttttccatt attattaaat
    18361 tgcacatcaa tacctttggt accattgctt cgtgcaaatt tcgacatcga atacaaacaa
    18421 taggctgttg tttgcgtact catccactcg ctgcttgaca tttgtttggc caatttgctc
    18481 gccatcgaaa aagcattttg tttttgtccc aacaaaatca aggtttccaa tgtcattgcg
    18541 cgattcctgt cgccagaacc ataatagtaa taattgtatc tggcatcaga ctcgtctatt
    18601 ttgctttttg ccaacagagt caatcctgcc gctttttgtc ctgccaaaac ataagcagaa
    18661 gccaaacgca atttactttc attcgaaatt cctgccgttt ccctcaatcg attcatcgaa
    18721 gacaaatctg gcgagcctgc caatgccaaa gtataaagtc ggtaagcttg tgccaaatca
    18781 ttgccgtatt gtgccacaaa tcgccattgc ttggcttgtt tttgttggta tgaaatccat
    18841 tttgacttga aattgatagg caaaacatag ccttttttct cggcttctat caagaaatgt
    18901 ccggcatagg aacttcccca atcatccgct gttggttcac cttgccaata ggagaatcct
    18961 ccattagaca attgaaaact acccaatctc gtgattccag cagtgacatt tttctggatt
    19021 aaatctttac gattagcatc aatatcaacc acatcattca agaataattg tggaaaaaca
    19081 gcggaggttg tttgctctac acaaccgtgt ggatattcta tcaaatattg caaccttctg
    19141 ttgaaatcga tggtgggcat tgacgaaact tcgagtttgg ccttgttact tccagcgact
    19201 ccaaaagttt tccaagcaat cgttttggaa ctattgggag gcaaaaccac atctgtaaaa
    19261 gtactcgtta ccggatttgg attggtcata tccatttcga catcataagt cgatttcgcc
    19321 gctcccgaac tggcaatgat ttgcactttt ccaattcctg ttgcattgcc gacttctaaa
    19381 ttgaaataca ccattttttc gtcaggttgt gcaaaagtta gattttgaac agcacttcca
    19441 accaccttca aaccattgtt ggttttgatt tgaacggtca cgtttttgat atttttttcc
    19501 attgtaaaaa cagtaactgg aatggtcact ttttccgaag gagaaatttt tctaggcaaa
    19561 gaagctaaca ccatcaaagg gctacgaaca ggcgttgctt tttcgacgct tccgtaagca
    19621 cttgaattgg catcggctgc cacgaccata gttcttactg aaccgatgta tttcggcaat
    19681 tttatttgat gtgttttgga ttgtcctttt tctaacttga atggaccgta atacaccaca
    19741 acaggtttga aacgattggc ttttttggct tttccaccgc ccaaatcttg gtcgccacca
    19801 atactgaaaa tctggttgac ttttccgcca taggctccga taacctcatc ataaacatcc
    19861 caggttttta cgcccaaagc ttcccgaaca taaaaagcat cccaggcatt tggagtctta
    19921 aaacgagtta aatccagtag gccttcatca acaatagcaa tagtataagt catagctttt
    19981 cctgtttttt cgcttacctt gatattaaac tgctgttctg gtctcaaaac atccggcata
    20041 gccaacttcg gttcgaggat ggtatttttg tcaatcactt caatcggaat aatgccgtac
    20101 attctgattg gcaaatcgtt tttagtcgaa gcgtgcggtt gcaacaaagt aatgttcaaa
    20161 taaacgttgg gcgccattgc agccgtaatc gggacttcga ctttggtttc gccttgttgg
    20221 gtctttgccc aaagcgtttg catcactttc gaaccgtttt cgatggaaat caaggcgcgt
    20281 ccaccctcgc ttgaaggaaa ggaaatttgt gctttttcgc caacggcata atttttttta
    20341 tcggtagaaa aaaccagcat attcgcagtt gaagcatcgg tgtttctggt ttttcctgac
    20401 caaataggcc aatcaatatt tactgccaat gccgttgcgt ggccttcgtt ttcatccgaa
    20461 acccgaatta aataacgtcc ccattcttca tcattcaagg caaattgcaa aattcctctt
    20521 ccgtttgctc ccgtatttaa ttggaagctt ttataagcag ttgtagcgtt agacgaatta
    20581 taatttgata aattatcgct cgaagcatcc caccaccagt gccattcgac tttatatatc
    20641 ttgatttcta aattttttac cgctttcgga tacccatttt catctactgt aataatttcg
    20701 aaacggttgt tttttcgagt ttcaagcatt tcatatttgt tcagctcagg agattttatt
    20761 cctacatagg ttttgtatgg agaataagta gttgccatta catcggtact gaaatcacca
    20821 ccttcttcat acactttggt gataaaggag gctttcaaca ttcccggtgc ttgaccttgt
    20881 aatttgggct ggatatttac cgacgctctt ccattagcat ccagttttcc agaaaaaata
    20941 ttaatttctt cggtgctgaa tttgcgcacc atatcatcaa aaatatagtt ggaataattt
    21001 ttaaaagtag tggtttgttg agtaaatttt gcctgcattt ctactttcaa atttttggca
    21061 acagccccgt gaagccaagc cacttcaagg ttacttgtgt tgggatatga agaagaaagc
    21121 atggcgtttt tgaaactatt tttaatcttt agacgattgg gttttatggt ttcaattttg
    21181 atgcttttat aaaattttgc tccgcccaca ttcgccatcg cttcccaatt tccggttggt
    21241 gcatcggaat tggtgggaac aacaaaaaga taatgattcc attcatttga cctctgaacg
    21301 gtttgacaca ccacttttcc gtttggatca gacaacctga attttatggg atgtgaagat
    21361 ggtaatttat tctctgcatc attcaaaata aacgacaaat ataatttatc gccaggacgc
    21421 caaacgccgc gttccccata aatatagcct ttgagtcctt tttgcaaggt ttcgccagcc
    21481 acatcaaaat tactgaccga cagtgaatta ccatcatcaa gcttaacata agtcgattga
    21541 tctcctaaag tgacaatggc aaaataagca aatctatcca attgaaaatt ggcaatccct
    21601 tcgctgtccg tagccacggt agccaatttt tgttgctgat aactatacaa atcgacagtt
    21661 gcatttgaaa cgggctcggt ggtcacgatg ttgttcacgg caacgaaata agatttattt
    21721 tcaccacgtt ttgcaataac tcccaaatcg gaagcgagaa tattagttcc tattttggta
    21781 ttgcggtaat aggattttgt acacggattg tctctgtctt gccaattgta atcgtcgtca
    21841 taataatcgt catcatagta attgcctcca ctataattaa catcattttc atcaacaaca
    21901 tcttcttctt cttgattttc atctaccgaa ggagattcgc atttgtataa agaataggcc
    21961 ttcttaaaac tgaattcaac tcgataaata gcccctggtt ctggcgtgat tatcttggac
    22021 aagtctagtc caaaggtgtt ccatcggtca aaattcaaca gtttattttc ttttaaattc
    22081 aaggtggttt ttgcaatagg ttgtgcgaca cgtttcaaat attgagtccc attcaactcg
    22141 ttgtcttgca gaaattggag aatgttgctt ttgtaaattt tatagacttt cacatcaaca
    22201 gcgctcaagt ttactgcttc gaaattaagc ttcaaattat ttgaactggg caaaatagtt
    22261 ccatttttca caaaacgcac actcggtttg gtttgttcga aagagacttt tgtggcaaag
    22321 tttgtcgcca ttttttgccc ttcatcattt tcgattcctt gaaaagcttc gaccaaaagt
    22381 accccattaa tttccttcga aggttttcga tttccatatt catcgacttc ttcattcaat
    22441 ttatccgaac cgtcattatt gaaaaacact ttcaataaat tgccttgcac cacaaatttt
    22501 agattattgg tattttcgat agctaccaaa ccactaaaat cctgaccgtt ttgcaaaggg
    22561 tcggaaaaat tgatatacaa actttgaacg ttcccttctg gaatctgaac tttcattact
    22621 ttgaaaacat cctttgccgc aatgacataa tccacacttc ctttttggtc aatgttaaaa
    22681 tcgctgccgt catacgaaat ttccacttta ctttcttctg gaaaacgctg aatactgtca
    22741 ataataaact tgaattctgt tccagagctc actgttttgt cgaattttat ttttaaattc
    22801 ttgccttttt gctcggccga aattagttgt ttagccgttt ccaaatcgat gttgtcggcg
    22861 gttttcaagg ttccattcaa atactggaaa tctttattat aggattgaac atcgtgggtt
    22921 tccacggcaa aatcctgttt gatggtttta atggtaaaac gaaattcgga gagttctttt
    22981 ttgacggaaa tcaatttatc taaattcaat gtgatttgat attccgtgtc cgatttcaac
    23041 ttttcttcgg gaataaaagc aatggtgttg ttggaaagtg ccaccacttt tccatcaaca
    23101 tttggcgaaa tgtcaaacaa atcgctgtct aaaacctgat tggctttcca ttctttttta
    23161 tcgaaagcca aaactaccct aatatccgag gtggtcgaga cgactcctcc ggtaaaactg
    23221 tttatatact ctttaaacaa tgaaaaatca gaattgaaat cggatgattt cttgctacac
    23281 gcttgaaata caaaaaaaac aaaaaacacg taaactaatc cttttgcttt catttttata
    23341 aagagttaat gggttacgaa aaaaaatgtg tgacgccact aaatagcatt ccacataaaa
    23401 tgcacaactg cgaaatagca gtatatatta tagtaaaagt atattatttt taaattgctt
    23461 taagaagaat tttcagaata aataaaaaaa atttttacct tcttaacttc aaaaaaaaca
    23521 tcaataaatt gactccataa cacaacaaaa taagtaagtc gaaagtttaa aaatcggaaa
    23581 ataaaaaacc taaaaatcgc taaaaattaa tgattatcaa ttatttacac aaccttaaga
    23641 caaaaaaaat gagcccaata caccatcgag ttcattctaa tacattcagt aaaaacttat
    23701 ctaatattca aaattctaaa tcattttctc ttattggcaa aaaaaacgct tcatcaaatc
    23761 ggcagtttaa gataaccttg tgcggtttca aagtctatgg cagtaaatca aaatcagtat
    23821 aaatatcctc gttttcaagt ttacgaatta catattcttg caactccata aaactacaag
    23881 ggcgacttct taaacggtcg ataatgtata aatacctttt gaaataaatt aattttgaca
    23941 taatgattat gatttatttt ccaaagataa atcatttgtc tgccaatagg tggcggggtg
    24001 aattaataac atctagaaga tataattttg ttttttctaa taccaaaatt tattttctta
    24061 acttcgtttt tttctaataa aacaaaatct acacataatg aacaaaactt ttcattcgct
    24121 actaacactg ctttttttag cttttttcac caattgttat tctcaaaacc cacttaactt
    24181 tggcagtaac atattgacag cggatccttc tggtcatgtg tggaaagacg gcaaaatgta
    24241 tgtttacacc tcacacgacg aggaatgtca gaaagatttt tatatgaagg actggcatgt
    24301 tttttcgtca agtgatttag taaactggac tgatcatgga gcgtgtctgt ctgttaacga
    24361 tctttcatgg gcagataatt ttgcttgggc acctgactgc gggtataaaa acggcaaata
    24421 ttatttttgt tttcctgcag gaaatggttt taaagaccga gtaaactcaa aaaacagcac
    24481 caaatggatg ggaattggaa ttgcagtaag cgattcgcca acaggtcctt ttaaggatgc
    24541 tatcggcaaa ccactttgga caaaaccgta tgcaaacgac ccttgccttt ttattgacga
    24601 tgatggaaaa ggttatgtat attttaatgg tggtggcgat tatctagtag ccaaattagc
    24661 cgatgattta cttagcgtca aaggcgatat tcaacctatg aatatgggtg gatataaacc
    24721 aaaaagagaa ggtccgtggg tttttaaaag aaaagacact tactattata cgatgccaga
    24781 aaacaataga atccttacgt attacacttc aaaaacgcct attggtcctt ggaaatacca
    24841 aggcgtaatt atggattctg aaggcgaaag taacaaccat cactcgatag ttgaattcaa
    24901 aggaaaatgg gttttattct accatagatg ggttgaaaac aaatcagaat gtggagaaaa
    24961 acaacgcaaa gtatgtgccg aaaacctata ttttaatgac gatggcacca ttcaaaaagt
    25021 tagtagaacc gatgcaggaa ttggcaattt gtttcgttaa aaaaaaaaac gaattgtttt
    25081 atttacatca ttaagattct atcacttcat tttcgcttcg ttgcaaaaaa acgcttcatc
    25141 aaatcggcag cttcattggc cataactcca gaaacaactg tggttttggg gtgcaattgg
    25201 gttcccattg tcataaaacc ccgttgctca tcgcgagcac caaaaacaat tttcgaaatc
    25261 tggctccaat acaaagcgcc ggcgcacatt tggcaaggtt ctaaagttac atagagcgtg
    25321 caatctttca ggtattttcc acctaagaaa ttggcggcgg cggtgatggc ctgcatttcg
    25381 gcgtgtgcag tgacatcgtt aagtaattcg gtcaaattat gacttcgagc aatgactttg
    25441 tcggctacaa caataatagc tccaactgga atttcgcctt tttcaaaagc catttcggct
    25501 tcttgcaagg ctttcttcat aaaatagtcg ttggtgaaag ggttttccat agttacaaaa
    25561 ataagaattc aattcgtacc tttgccttat gaccaagaat ttacttcaac acatcaacaa
    25621 tccaagtgat ttacgtcaac tcgatgaagc ccaacttcct cagttagcac aagaattgcg
    25681 tgattttatt attggcgttg tagcagtaaa agaaggacat ttgggtgcca gtcttggcgt
    25741 ggtcgaactt acgattgggc tgcattatgt atttgacaca ccgaatgatt tgttaatctg
    25801 ggatgtgggt catcaagctt acggacataa aattttgacc gaaagaagag aaatttttca
    25861 taccaatcga caactcaacg gcatttcggg ttttccaaaa cgcagcgaaa gtgtgtatga
    25921 tgcttttggc gtgggacatt cttctacgtc tatttcggca gctttaggaa tggcgattgg
    25981 ttctaatttg aaaggcgatt tcgaaaaaca gcatatcgca gttattggtg acgcttcgat
    26041 tgcttcggga atggcgtttg aaggtttgaa tcacgccggt gttaccgatg ccaatttgct
    26101 ggtcattctc aacgacaacg ccatcgggat tgacccaagc gtgggtgctt tgaaaaagta
    26161 tctcactgca gtgaaagaag gaaaaaatcc gaagaaaaac aacatgatta aatccctgaa
    26221 ttttgattat tcgggaccca tagatggcaa cgatatcttt gcagttctta aagaattaaa
    26281 gcgattacaa aaaataaaag ggcctaagtt tctacatcta ataaccacca agggaaaagg
    26341 tttacaacaa gccgaagaag atcaagtaaa ataccacgcc cctggaaaat ttgatgcttc
    26401 gactggggaa attcacccca aacaggaaga aaatcttccg ccgaaatatc aagatgtttt
    26461 tggattgacg cttttggatt tggcgaaagc caatgaaaaa atcatcggaa tcacaccagc
    26521 aatgccatcg ggaagttctt tgaaatttat gatggatgaa attccagaac gcgcctttga
    26581 tgttggaatt gctgaacaac acgctgttac cttatcagcc ggaatggcta cacaaggaat
    26641 ggtggttttt tgcaatattt attccacttt tttacaacga gcttacgatc aagtcattca
    26701 cgacgttgct ttacagaatt tgccggtgat tttttgttta gacagagcag gattggttgg
    26761 cgaagacggt gcaactcatc acggcgtttt tgatttggct tatttgcgtt gcattcccaa
    26821 tatgattgtg tattcgcctt tgaatgaaat tgcgttgcga aacattttat atacagctca
    26881 attgggattg aatcatccta ttgcgattcg gtatccaaga ggtcgtggtc aaattgtgga
    26941 ttggcaaaaa ccgtatgaaa aaattgaaat tggaaaggct aattgcctca aagaaggaac
    27001 aaaaacagca atattatcta acggaacgat tggcaataat gttacattgg cattagccga
    27061 aataaatcat cccgaaaatt tcgcacatta cgactttgcc tttgtaaaac cattggacga
    27121 gaagttgtta cacaccattt tcaaatcatt caaaaacatc atcaccgttg aagatggtgt
    27181 tgtaaaaggt ggttttggca cgactattgt agagtttgcg tcgaataata attataactc
    27241 cagtattaga acacttggaa ttcctgatga atttattgag caaggtactg tttacgaatt
    27301 acaacattat tgcaaaatag acgtttcagg tttgaaaacc gtttttatga attattaaaa
    27361 cgagttattt tttgtaaata taaattttag tcaataatga aattatattc ttttagcctc
    27421 ctattactca tcctatttgt tccaatgatc ggcttttcac aaaaatcaaa cctacattca
    27481 ctaaagcctg taatttatgg agattcttta agtgttggtg ttgtacaaga ttccataaag
    27541 cctattgtag ccttagacgt tgcaattaca ccaataataa aatatacctc tccaccaaaa
    27601 tcaaaaatgg cagttgcaaa aaaaatagac attccattta tttcaatgtc acattggatt
    27661 aataaaaatt cggttggtat tgatttaaca caaattgctt ttgtgaactg gaatgcaggt
    27721 ggaactagct cagttgcagg cttgtttact ggaactttta cgagaacacg atcggatatc
    27781 aatagcgaat ggattaatga attaattgtg aaatacgggc tcaataaaca agatggtttg
    27841 gatatgagaa aaacagatga tgattttcgt tttaactcta cttttggcta tagacaagat
    27901 actttttcga attggtattt tagtgcaaaa ttcaatttta gaacccaatt tacagatgga
    27961 tataaatacc ctgataccaa aaaccctatc tcaaaaacat tcgctccagc ctatactttt
    28021 ttgggtgctg gtgctgatta tttcaataaa gaaaagaaat tcgatgttta tatttcgcca
    28081 ttgactttaa aaaacacttt ggtattagac caaactctgg ctgataaagg tgcttttgga
    28141 atgatcaaag caacttatga tacagaagga aataaactta cttctggtga aaaatccaga
    28201 actgaagttg gaattttagt aaccaactat tacaaaaaag aagtttggaa aaatattact
    28261 tgggaaaata ggctaagttt atataccgat tacatcaaca attttggaaa catcgatgta
    28321 gattggaaaa tgcaattaga tttaattgta aatcaacatg ttaaggcaaa cattggctcg
    28381 cagttaattt atgatgatga tgtcaaaaat attgaaaaaa tagatggtgt tcaggttgtt
    28441 tcaggtgcaa aagtacagtt aaaacaatct cttggaattg gaatggtcta taatttttaa
    28501 aaacagcaac ttataaccct tgatctcaga ctattttttt tcaattatta atcacaaatt
    28561 taaaagtgta attcgtgaat taaagatgtt ttaagcaaac aaattcaaaa ctattcatct
    28621 atatttgact agtcctaaat aatcgataca gattattaga caaatttaaa taaattaaa
//
LOCUS       DAOSNN010000006        28160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_1692_length_28160_cov_6.496993, whole genome shotgun sequence.
ACCESSION   DAOSNN010000006 DAOSNN010000000
VERSION     DAOSNN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 28160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28160
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1692_length_28160_cov_6.496993"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..878
                     /locus_tag="PLQ70_00590"
     CDS             174..878
                     /locus_tag="PLQ70_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33142.1"
                     /translation="MKKENLIELDNGNLALIEQMRNRPGEIILEELIQQAFKIFANKL
                     ANGGIIAENEFAFQFELGTILKTLGQLYEFKLEDKFHLEFETNISLNKESSKSKTKQA
                     RIDLLIRYELDNKTIQAAIELKYFRKKNNREPNNRYDIFTDISNLESYKKNNIEICYF
                     LLITDHQHYVDKETYSQDTSDFDVRHGKTYKANTVLSYRTPKKPKKYEADIILTQDYD
                     FGWKTLGNYYFLILKV"
     gene            1068..2072
                     /gene="recA"
                     /locus_tag="PLQ70_00595"
     CDS             1068..2072
                     /gene="recA"
                     /locus_tag="PLQ70_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQE33143.1"
                     /translation="MSSEKEAKLKALQLTLDKLDKTYGKGTVMKMGDKAIEEVETISS
                     GSLGVDLALGVGGYPKGRVIEIYGPESSGKTTLTLHAIAEAQKAGGIAAFIDAEHAFD
                     RNYAEKLGVDIENLIISQPDNGEQALEIAENLIRSGAIDIVVIDSVAALTPKSEIEGE
                     MGDSKMGLHARLMSQALRKLTGTISKTNCTVFFINQLREKIGVMFGNPETTTGGNALK
                     FYASVRLDIRRSTQIKDGENVLGNRTKVKIVKNKVAPPFKTAEFDIMYGEGISKTGEI
                     LDLAVEFEIIKKAGSWFSYGDTKLGQGRDAVKALIKDNPELADELEEKIKAKIKENNA
                     "
     gene            complement(2199..2945)
                     /locus_tag="PLQ70_00600"
     CDS             complement(2199..2945)
                     /locus_tag="PLQ70_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQE33144.1"
                     /translation="MRGIKIIFWALWRVWFYVLMAIPILVMLPFLVLSILTERGYPCF
                     FKMARIWAKFILFGMGFYYKIEREQEFEKNQSYMLIANHTSMADIMLMLATVRKPFVF
                     VGKKELVKIPIFGFFYKRTCILVDRLSSRSKHGVFERAQKRINQGLSICIFPEGGVPN
                     DESILLDHFKDGAFRLAIEHQLPIVPMTFGDNKERFSFTFLSGSPGIMRVKVHSFIET
                     EGKTMADKNSLKDQSWNTIHTQLLDFQKKK"
     gene            3217..4188
                     /gene="trpS"
                     /locus_tag="PLQ70_00605"
     CDS             3217..4188
                     /gene="trpS"
                     /locus_tag="PLQ70_00605"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQE33145.1"
                     /translation="MAKILTGVQSTGTPHLGNLLGAIIPAIELSKNPANESFLFIADL
                     HSITQIKDGKTLRANTYSTAAAWLACGLDVNKVVFYRQSDVAQTAELSWYLSCFFPFQ
                     RLTLAHSFKDKSDRLEDVNAGLFSYPMLMAADILLYDAQFVPVGKDQLQHLEITRDVA
                     SRFNHQMGETFVLPEAKIQEDSMLIPGTNGGKMSKSANNIINIFLDDKALRKQIMSIE
                     TDSTPLEAPKNPDTCNCFAIYSLLANEEQLEQMRANYLGGNYGYGHAKQALFELIVEK
                     FKTEREKYNYYINNLAEVDALLKIGAEKAGVVASGVLARVREKLGFE"
     gene            complement(4254..5354)
                     /gene="dprA"
                     /locus_tag="PLQ70_00610"
     CDS             complement(4254..5354)
                     /gene="dprA"
                     /locus_tag="PLQ70_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQE33146.1"
                     /translation="MTDQDLFHLLALQQVEGVGDIVAKKLITHCGNAVEVFKTKSSQL
                     ASIDGIGSVLLKNLKDKTVFEKAEQELKFIKSNEIDVAYFQDENYPDRLKHCIDGPVL
                     LFTSGNIDLKNKKIISIVGTRKITSYGMEFCRKLIEDLAPLDPVIVSGFAYGVDIFAH
                     QLAMEHDLQTIGVVAHGLNQIYPKDHKKYVAKVEKNGGFMTEFWSTSNPEKENFVRRN
                     RIVAGMTEATIVIESADRGGSLITANIANDYNRDVFAVPGRTTDKYSQGCNNLIKTQK
                     ANLLTSAADLVYILNWDIESKAKPVQKQLFVVLENDEQKVYDYLLKNGKELLDIIALR
                     CDFPIYKISGMLLNMELKGVIRPLPGKLFEAI"
     gene            5457..6398
                     /locus_tag="PLQ70_00615"
     CDS             5457..6398
                     /locus_tag="PLQ70_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQE33147.1"
                     /translation="MNIELYISQLLYRYQCVTVPGFGAFLTEIQSAQLIESSHSFFPP
                     KKMISFNAHLKNNDGLLANHIAQAEKTSYEYAISAIQYEVFNWKKALQENGLLSIKNV
                     GNFSLNADKNLIFTPYDQTNYLTNSFGLSAFVSPVVKREIFEQKIEALIEEEKEVIAF
                     EPEIRTSRPYLKYAAILILGLGLTGAIGYPVYQQQIENQTIIVEKSVQKQVQNKIQEA
                     TFFIENPIPAVTLTVKEKKMSYHIMAGAFRDEKNAQKKFKELSNLGYKARRIPQNKHG
                     LYPVLYGSYRTFAEANKAKEEIIKTDNPDAWVLIESL"
     gene            complement(6489..8693)
                     /locus_tag="PLQ70_00620"
     CDS             complement(6489..8693)
                     /locus_tag="PLQ70_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HQE33148.1"
                     /translation="MEIIVYECLKGFHRRFIESEKNPELQNINAFADIMQGDFSGKNL
                     IGKIIATDEYWQKMLNTKGFGRGVKYDKVLDYRKKLTEAWNIIKETFDTKNEVEISKL
                     MGLYLWDMEEDKFTHSYLGVIEEAIKQNKLWKNPAVSDIFFTFLVFIPNALKTLDEIA
                     QKNNLDRERVRQLKVESLNNFEQDFWFLKDTLIKEKLENIFDVSSHNFLQIREQTKMV
                     NEAEEVHFTSHFYTKTLSIVFDMLLIGDINEITNKNKRNSNGNIWSNLYLQTQLENER
                     FDIKRVIDSLAIEMYKNNDRFVQDKVIDISGYATIPLTQFEIESYNSIIAEELILKTE
                     IKEDKVIIKRTSHITQPEIVETVLRELGGFAYADDILKKVIENHPEKDWTMPVLRTSL
                     LRGEVFYSVGKSALFGLNNVKDVRDQMGSGTLNDVISIYMSRKDSPIHIHELLEHINN
                     LFPRPKKLLTVQAILEQDSKQYFVKFEGGFYGLGEKDYQNTEFPKVKGGHGKYLKQII
                     KISNGIGFEKLSEDFNNRYGLLEIQVKYLLDLMIETYGVSLKDGLYYPFTALEIPETV
                     ENDNFEISAEETDLEIDEEELDFDELNQPDVPEEFIKDVLAQIKVRRGQPKFRQKLLK
                     LYKRTCIVTGCKIPELLEAAHILPHSVKKDFSLSNGLLLRTDIHTLFDLDMIAVNPEN
                     MRLKLKSTLIESSEYAGLNDIDIGLRITKLHQSYKISEEGLFRRWETFIESE"
     gene            complement(8900..11167)
                     /locus_tag="PLQ70_00625"
     CDS             complement(8900..11167)
                     /locus_tag="PLQ70_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQE33149.1"
                     /translation="MQTISEAAVYTLKFINQTHRSIFLTGKAGTGKTTLLREIIQTTH
                     KNTVVVAPTGIAALNAGGVTIHSMFQLPFGGFIPDNSSPHFSESTKFETKATLRRHFR
                     MSGLKKSVIQNMELLIIDEVSMLRADLLDAMNFMMQTVRKKTTPFGGVQVLFIGDLLQ
                     LPPVIRDEEWRTLKTYYKGKFFFHSHVIQQNPPLYIELDKIFRQRDEVFISILNNLRN
                     NQISPADVQALNQYVNPNFDLKANKGYITLTTHNAKADAMNTQALEDLEGKEQVYKAV
                     IVGDFPDKIYPVDEELKLKVGAQVMFVKNDLSFDKKYFNGKMGIIKSLSKDEILVHFP
                     EENTTIEVEKYEWENIRYKVDELTKEIEEEVLGTFVHYPIKLAWAITVHKSQGLTFDK
                     AALDVSQVFLPGQAYVALSRLRSLNGLILLSPLRMNGISNDQDVMDYSLNKASEEVLK
                     NSLHFETKNFIHNYLINTFDWSDLAQEWRNHKFSYNESSDVSVKSKQASWAAKQLLII
                     DGLLDPSQKFIIQLNKIFNTETVDLYHVSERFTAAYNYFIKPMDDLVFEILWKVEEVK
                     RVSKAKAFYEELMVLEELQVKAVLRLMKAKLLIETVVAGEAISKEKLTSDVIKKYISH
                     KTEAIQKQFKELNVTLIEDEADVNRYTKKKKETKETKKSTVQETYELWLENNTIAEIA
                     AIRKFTPETILSHLTKLISAKTISVNDVLPEDKIKDLTEAFYGYKEESISPLKEKYGD
                     KFSWEELRMFKASLN"
     gene            complement(11406..11963)
                     /locus_tag="PLQ70_00630"
     CDS             complement(11406..11963)
                     /locus_tag="PLQ70_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQE33150.1"
                     /translation="MKKIVVILAIAISIVSCNNGEKSETKVKEVKTAYVDTSVLMKEY
                     TAAKDVEAKYKAKGEEKGRQLQAEINRFKQEAQNFQAQAQANGQEWAQKKGAELQRRE
                     QQLAQAQQMLAQQLQQEGGVEMDSVIVGVKKFIKGYGKEKGYAYIYGTGDSNATILYA
                     EDKADITKEIVKLLNEKYKASAAKK"
     gene            complement(12211..13059)
                     /locus_tag="PLQ70_00635"
     CDS             complement(12211..13059)
                     /locus_tag="PLQ70_00635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQE33151.1"
                     /translation="MNISNKKHFLTVKDYSVSQETFDLYYDETLDMLITHPQPSLENL
                     GKYYESEDYISHTDNKRSLFEKLYHFIKGIALKNKLNLINSQQSNKGRLLDIGAGTGD
                     FLLVAKNDGWQTIGVEPSDRAKGIARSKGISFVEETSELESHSFDVISMWHVLEHVTD
                     LDKQIKELKRLLKPTGTLIVAVPNFKSFDAQHYGKFWAAFDVPIHFWHFSKTAIQLLF
                     EKEEMKLEKVLPMKFDSFYVSLLSEKYKSGKMNFVKAFFTGLQSNWKAKSNFEYSSHI
                     YVLKNK"
     gene            complement(13184..15055)
                     /gene="mnmG"
                     /locus_tag="PLQ70_00640"
     CDS             complement(13184..15055)
                     /gene="mnmG"
                     /locus_tag="PLQ70_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQE33152.1"
                     /translation="MFLEEYDVIVVGAGHAGCEAAASAANLGSKTLLVTMSLQNIAQM
                     SCNPAMGGIAKGQIVREIDALGGYSGIVSDKTAIQFKMLNKSKGPAMWSPRVQSDRMR
                     FSEEWRLMLEGTPNLDFYQEMVKGLLIEYGKVKGIKTSLGIEIRSKTVVLTNGTFLNG
                     LIHIGEKQFGGGRAGESAAYGITEDLVNAGFESGRMKTGTPPRVDGRSLDYSKMNEEK
                     GDAKPDKFSYSDETKPLIHQRSCYMTYTSLEVHNVLREGFDRSPMFNGRIKSLGPRYC
                     PSIEDKINRFADKERHQLFVEPEGWNTCEVYVNGFSTSLPEDIQFKALRSVVGFENVK
                     FFRPGYAIEYDYFPPTQLKHTLETKLVEGLYFAGQINGTTGYEEAASQGLMAGINAAL
                     KAQEKAPFTLKRDEAYIGVLIDDLITKGTEEPYRMFTSRAEFRTLLRQDNADFRLTPM
                     SYEIGLASEKRLHRMEHKLNESEKMVAFFKETSVTVAEANPILESKDTALITQGDKMF
                     KVFSRPQIDLEDIIKFEKVATYVAENNLDQEILEQAEIQVKYSGYIEKERNNANKLSR
                     LEDVKIPENFDYSKIKSMSIEARQKLSKIRPVTISQASRISGVSPSDVSVLLIHMGR"
     gene            complement(15244..15663)
                     /gene="ybeY"
                     /locus_tag="PLQ70_00645"
     CDS             complement(15244..15663)
                     /gene="ybeY"
                     /locus_tag="PLQ70_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQE33153.1"
                     /translation="MIDFNYETEFVLDNEEAIANWISKAITSENKTEGEINYIFCDDD
                     YLHKINLEHLSHDTLTDIISFDYTMGNEISGDMFISVERVKDNAIDFNVSFEEELKRV
                     MVHGILHYCGYKDKTEADALLMRTKEDEKIAMFHVEQ"
     gene            complement(15660..18980)
                     /locus_tag="PLQ70_00650"
     CDS             complement(15660..18980)
                     /locus_tag="PLQ70_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33154.1"
                     /translation="MEQSNFIYQKLELFIRKFYTNELIRGMIFFIGLGLLYFLFTVFV
                     EYFLWLKPVGRTLLFWTFIGVEIFLLFGFILFPIFKLFKLQKGIDYKEASAIIGNHFT
                     EVNDKLTNFLQLSNDQNQSELLLASIEQKANALKPIHFGNAINFNTNRKYLPLAILPI
                     MFFAFFFVSGNSTIISQSLNRVVHFREQFLPPAPFEFVLLNSNLQTEQNQDFIVRIKT
                     QGKIVPENAMIFIGDESYFMESTKPGEFQYRIEKPSSNILFHVEANTISSSDYELNVV
                     SVPSIANFEMLLNFPAYLNKKPEIIKGNGNAILPEGTRVTWRINALATQNVVWKNASS
                     NASFAKTENNFSLSKQIVENTEYQILTSNSKVKNYEKLNYQISVIKDQFPTINVNNAP
                     DSLKVDKSYVLGQISDDYGLSKLQIVYYPKNKPESAKRGTIAVKSNLYDQFVFAFPSS
                     LPVEQGVSYEYYFEVFDNDAIHNFKRTKSSVFSNRIATDEEKEDQDFQQQNESINGLE
                     KSLKNQDKQISEMDKLQKAGKEKDNLEFKDQQKINDFIKRQHQQDKMMKDFAEKMKDN
                     LDKSKTGKKDEFKEELQKRLENVDKDLEKNKKLLDELKELNDKIKQEDLMEKMDKFKQ
                     TAKNQTKSLEQLVELTKKYYVQKKAEQLKDKLDKLADKQEDLSNREKENTKENQDEIN
                     KEFDKIQEDLKDLDNENMELKKPLYILDDPAQEKSIDEDLKKASDELQKNSKSSAKPK
                     QKSAAKKMKQMSQKMEQEMESGEKEELEEDISMLRQILDNLLAFSFSQEDLMGKFKGY
                     KVGSPAFNKALKNQQDLKLQFKHVDDSLFAMSLRNPKIEENITKEVGNALYNLDKSLE
                     TLADAQIAKGVSHQQYTVASANKLADFLSDALNNMQMQLSGSMGAGKPKKGDGDGMQL
                     PDIIKKQEGLAEKMKDGMKKGQKPGDKPGQGKQGEKGEKGKSGKGSGSEGENGEDGEG
                     DAQAIMEIYKEQKQLREALQNELNKRGLGGNGQNALDQMKQIEKQLLNKGFKNENLQR
                     ILNVKQELLKLNTAIQQQGQENKRQAETNKKEFTNQSKPLPSALLDYLNSVEILNRQS
                     LPLRTNFNQRVQEYFNKK"
     gene            19145..20647
                     /gene="gltX"
                     /locus_tag="PLQ70_00655"
     CDS             19145..20647
                     /gene="gltX"
                     /locus_tag="PLQ70_00655"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQE33155.1"
                     /translation="MSRPIRVRFAPSPTGPLHIGGVRTALFNYLFAKKNKGVFFLRIE
                     DTDQTRFVPGAEAYIMEALEWLGIAPDETIGKNEKFGPYRQSDRKDLYVQYAQQLIDS
                     DWAYYAFDTPESLDFHRKQHEAEGKTFIYNHHNREKLDTSLVISAEETAKRIANGEHY
                     VIRFKTPVDETLHLKDIIRGEVKFETNLLDDKVLFKSDGMPTYHLANIVDDYLMETTH
                     VIRGEEWLPSMPLHVLLYKAFEWEAPEFAHLPLILKPVGNGKLSKRDGDKLGFPVFPL
                     EWKTEEGVSSGYREKGFFPETVVNFLALLGWNDGTEQELFSLEELVEKFDLSRVHKAG
                     AKFDPEKNKWFNHQYLIKQNDADLAKSFASILEEKGISTALDLTKIVSLIKERAHFVS
                     EFWDLSDYFFQAPITYDEKARKNWKEETPALMQELISVLEEISDFTSLNIETIVKDWM
                     TKNEIGMGKVMQPFRLSLVGALKGPHLFDIVELIGKEETIQRIEKAIATL"
     gene            complement(20681..21319)
                     /locus_tag="PLQ70_00660"
     CDS             complement(20681..21319)
                     /locus_tag="PLQ70_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorT family protein"
                     /protein_id="HQE33156.1"
                     /translation="MKKIFLTSVLTLISTICFSQYEYRDSNRIGISFGVNQFTLNTNN
                     FQTKPGTGWNGGLSMRGNYYDNWDALYAMQFSENNFSVSTNSGFITEDTNYKLSSVQI
                     SFLFSYKIVENHLSVEIGPLVQINGKLSVEKDKQNNSISGTTLLAKDIVDISKFSFYP
                     VVGITAGVRHVRLNVSYQYGVNNMLGNLNNKNLGYNFKGNPGILNGNLIIYL"
     gene            21396..22379
                     /locus_tag="PLQ70_00665"
     CDS             21396..22379
                     /locus_tag="PLQ70_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQE33157.1"
                     /translation="MSPVLIIILVFALFIFFSSFFTVKQQTAVVIERFGKFIGIRQSG
                     LQLKLPVIDKVAGRVNLKIQQLDVIIETQTKDNVFVKMKVSVQFKVIPEHVYEAFYKL
                     EYPHDQITAYVFDVVRAEVPKLILDDVFIRKDDIAIAVKRELNEAMTTYGYDIINTLV
                     TDIDPDIQVKNAMNRINAAEREKTAAMFESEAQRIRIVAKAKAEAESKKLQGQGIADQ
                     RREIARGLVESVEVLNNVGINSQEASALIVITQHYDTLQAIGADTNSNLILLPNSPQA
                     ASDMLNGMVTSFTASNIIGEQMKKQPKREKSKNHTSTDYNPSDTSNPAEPA"
     gene            complement(22411..22665)
                     /locus_tag="PLQ70_00670"
     CDS             complement(22411..22665)
                     /locus_tag="PLQ70_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6327 family protein"
                     /protein_id="HQE33158.1"
                     /translation="MENKKYSSYAQIELELKILKIERELNLKRVVLNVEKTKESLLPK
                     NIVKSFLGDYKSIVSNNIGTIINIAIPILINWITKRKRGH"
     gene            complement(22669..23019)
                     /locus_tag="PLQ70_00675"
     CDS             complement(22669..23019)
                     /locus_tag="PLQ70_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
                     /protein_id="HQE33159.1"
                     /translation="MAFEELKEHTEDIQKEAQAYIENSIAYYKLWGFKVAMKSTTMIF
                     KFVLIAIALMMVLLFCSIAGAIAIGNALDSYALGFLIVGGIYLVFSGLLFLIKDKMVE
                     GPILEKFSEIFFND"
     gene            complement(23039..23389)
                     /locus_tag="PLQ70_00680"
     CDS             complement(23039..23389)
                     /locus_tag="PLQ70_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HQE33160.1"
                     /translation="MSNNTNSVLALLLGAAIGAGVGILFAPDKGSKTREKIKDGFDEA
                     KDNLKHKFEDVTHQLRKKFEGSKHNMEETYEEMVSNMSYKTEDVISFLETKLADLKEQ
                     NAKLQKKQLANHEN"
     gene            complement(23659..25782)
                     /locus_tag="PLQ70_00685"
     CDS             complement(23659..25782)
                     /locus_tag="PLQ70_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HQE33161.1"
                     /translation="MSTEEKSLHFIEQIIEDSLASGFPQSNLRFRFPPEPNGYLHIGH
                     AKSICLNFGLGLKYNAPVNLRFDDTNPAKEEQEYVDAIKEDLQWLGFNWAEERYASDY
                     FQQLYDWAILMIKKGKAYVDSQSSEDMAAQKGTPTQPGVDGPYRNRSVEENLTLFEGM
                     KNGDFPEGSHVLRAKIDMASTNMLMRDPLMYRILHRHHHRTGNDWKIYPMYDYAHGES
                     DYIEQISHSICTLEFVMHRELYNWFLDQIYDDTLVRPNQYEFARLNLNYTVMSKRKLL
                     QLVQENIVNGWDDPRMPTISGLRRRGYTPASIRKFCDVIGVAKRENIIDVSLLEFCLR
                     EDLNKTAPRVMAVLDPIKVVITNYPEEKEELLDAENNQEDENAGFRKVPFSRELYIER
                     EDFLEVAPAKFFRLSLGNEVRLKNAYIIKGESVVKDAAGNITEIHVTYDTDSLSGSGS
                     EASKRKVAGTLHWVSIKHAVEAEVRLYDRLFIDEAPDSHKEKNFLDFMNSNSLQIVKG
                     FVEPSLSIVKPGDKFQFQRLGYFNVDKDSTASKLVFNKTVGLKDAWEEKGKKEENSIN
                     NSLKDINKYFKVATREERIAIRKAIGETLAGISNYSLLQNSFKKNINNNKSSLLFANL
                     ILKYSSLKSSDFDNEDLTKLYLMSLRSESTFVRSRTLLNLQHLGYDSDFVNSFKEEIL
                     KLKSNPPKNTTERETEFIDQILNKE"
     gene            25893..26108
                     /locus_tag="PLQ70_00690"
     CDS             25893..26108
                     /locus_tag="PLQ70_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33162.1"
                     /translation="MRQVTVTIPDEYYDNLIEFLKPIPDAVVDNSQEEYNRSIEKMVL
                     ERVKNAKPEDYVDARESVQKLKLKYGF"
     gene            26098..26397
                     /locus_tag="PLQ70_00695"
     CDS             26098..26397
                     /locus_tag="PLQ70_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33163.1"
                     /translation="MVFKVQQQIRAELELDEIIYYYENIQVGLGNRFLIDYENLIDIL
                     ETYPFFEQKYNIIRKLPFKKFPYTIHFQIDEFEKIVYIESISCDYQDPNTTRIKL"
     gene            26400..26759
                     /gene="folB"
                     /locus_tag="PLQ70_00700"
     CDS             26400..26759
                     /gene="folB"
                     /locus_tag="PLQ70_00700"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQE33164.1"
                     /translation="MGIIKLKNIRTYSYHGCLVEEGKIGSDYSVDLEVKTDLRKSALS
                     DNLEDTVDYVLLNRIVEEEMAIRSQLLEHVAHRIIKRIFDEVAAISRITLAVSKLNPP
                     IGGDVEAVTIEMEEYRN"
     gene            26843..26913
                     /locus_tag="PLQ70_00705"
     tRNA            26843..26913
                     /locus_tag="PLQ70_00705"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26875..26877,aa:Cys,seq:gca)
     gene            complement(27054..27548)
                     /locus_tag="PLQ70_00710"
     CDS             complement(27054..27548)
                     /locus_tag="PLQ70_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33165.1"
                     /translation="MLHLELYYANIKDFSGIGKFKNLKRLELFNCLKLENDNEIDKLS
                     DTIEFLHINQSKKFKFTESLLKLKKLKVLCLNSCDSIENLDFLNKFPNLIDFRFVDTN
                     ILDGNLNQILEHPTIRTVGFMNKRHYNFKYENLKIELEKKNKTDYKTEVYNGEYSTFR
                     YEFE"
     gene            complement(27805..>28160)
                     /locus_tag="PLQ70_00715"
     CDS             complement(27805..>28160)
                     /locus_tag="PLQ70_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQE33166.1"
                     /translation="EIIKKDYLGLFIKGFFLNIINIGVLGFWLAVIISVGPKLEMQNS
                     RMLTFFTTVIVSYLLVDCIKIVLAKQLKTKMTPTNILKIKKGISIVLMIFGVALIVQG
                     WFPDVKKDVLEKIDK"
BASE COUNT         8958 a         5103 c         4484 g         9615 t
ORIGIN      
        1 tgagtttggt tttagcagga aaattaaacc ctcacgatgc cactgaattg aaggcgttgt
       61 ttgtgtgaaa atccatattt gaataaacca acttgatttt tatggattat gatagttttg
      121 ttagaccaaa ttatttaggt ttatgttacg caaaacttta aaataaagaa tgaatgaaaa
      181 aagaaaatct aattgaatta gacaatggta accttgcttt gattgaacaa atgaggaatc
      241 ggcctggaga aattatttta gaggagctta tacaacaagc atttaaaatt ttcgccaaca
      301 aactggctaa tggcggaatt attgccgaaa atgaatttgc atttcaattt gaattaggaa
      361 ctatcttgaa aacattggga caactctatg aatttaaact tgaagataaa ttccatttag
      421 aatttgaaac aaatatttct ttaaataaag agagttccaa aagcaaaacc aaacaagccc
      481 gaattgacct tttgattaga tatgaattag ataataaaac tatacaagct gcaattgaat
      541 taaaatactt tcgaaaaaaa aataaccgcg aaccaaataa cagatatgat atttttactg
      601 atatttcaaa tttagaatca tacaaaaaaa acaatattga aatatgttat tttcttctaa
      661 ttactgatca tcaacattat gtagataaag aaacgtattc gcaagacacc tctgattttg
      721 acgtaagaca cggaaaaact tataaagcta atactgtttt atcgtatcga acaccaaaaa
      781 aaccaaagaa atatgaagct gatataatat taacacaaga ctacgatttt ggatggaaaa
      841 cacttggtaa ttattacttc ttaatattaa aggtgtaaat ttaaatatag gggttattgc
      901 taattttata gttgtttttt atgaaaacta tactttgtgt ttttcgaaat tactttttca
      961 tactcaaaaa attataatca aaattatcct aaaacattcc ccttgcttct tccaaaaaaa
     1021 gaagtatttt taccaaagat aagtaccaaa aataaatcaa agaaaagatg agttcagaaa
     1081 aagaagccaa attaaaagcg ttacaactta cccttgacaa actagataaa acctacggaa
     1141 aaggcaccgt aatgaaaatg ggcgacaaag ccatcgaaga agtagaaacc atttcttcag
     1201 gctcattggg agtcgatttg gccttgggag ttggcggtta tccaaaagga agagtgatcg
     1261 aaatatacgg tccagaatct tcaggaaaaa caaccttgac attgcacgcc attgccgaag
     1321 ctcaaaaagc aggaggaatt gcagctttta ttgatgctga acacgctttc gacagaaatt
     1381 atgctgaaaa attaggcgtt gacatcgaaa acttaatcat ttcgcaacca gacaacggag
     1441 aacaagcctt ggaaattgcc gagaacttga ttcgttccgg cgccattgat atcgtcgtga
     1501 ttgactcggt tgctgcattg actccaaaaa gcgaaatcga aggcgaaatg ggagattcta
     1561 aaatgggtct tcacgctcgt ttgatgtcgc aagccttgag aaaactaact ggaaccatca
     1621 gcaaaaccaa ttgtacggtt ttcttcatca accagttgag agagaaaatt ggcgtaatgt
     1681 tcggaaatcc agaaacgact acgggtggaa atgccttaaa attttacgct tcggttcgtt
     1741 tggacattcg tcgttctact caaatcaaag atggcgaaaa cgtacttgga aaccgtacca
     1801 aagttaaaat cgtaaaaaac aaagtagctc cgcctttcaa aactgctgaa tttgacatta
     1861 tgtatggcga aggaatttcg aaaacaggtg aaatcttgga tttagccgtt gaatttgaaa
     1921 tcatcaaaaa agcgggttct tggttcagtt atggcgacac caaattagga caaggtcgtg
     1981 atgcggtaaa agccttaatc aaagacaatc cagaactagc cgatgaatta gaagaaaaaa
     2041 tcaaagctaa gattaaagaa aataatgctt aaaattagaa aagagtctcg caaatgtgag
     2101 actctttttt ttgtcctttt caagaaaaaa aaccgcaaat ttgcaaatta aatatccatt
     2161 taagattttt taatttgcga atttgcggta aatatttatt acttcttctt ctgaaaatcc
     2221 aataattgtg tgtgaatggt attccaagat tggtctttga gactgttttt atctgccatt
     2281 gtttttcctt ccgtttcaat aaaagaatgc acttttaccc tcataattcc tggacttcca
     2341 ctcaaaaaag taaaagaaaa acgttcttta ttgtctccaa aagtcattgg gacaataggc
     2401 aattgatgtt cgatagccaa tcggaatgca ccatccttga aatgatctaa aagtatcgat
     2461 tcgtcattag gaacacctcc ttcgggaaaa atacaaatac taagtccttg attaattctt
     2521 ttttgggcac gttcaaaaac gccgtgtttg cttctggaac tcaatctatc gaccaaaatg
     2581 caggttcgtt tgtagaaaaa tccaaaaatc ggaattttga ccaactcttt ttttcccaca
     2641 aaaacaaagg gttttctaac cgtagcaagc atcagcataa tgtcagccat cgaagtgtga
     2701 ttggcgataa gcatataact ttgattcttt tcgaattcct gttctcgttc gattttataa
     2761 taaaacccca ttccgaaaag aataaatttt gcccaaattc tcgccatttt aaagaagcaa
     2821 ggataacccc tttcagtcaa aatagacaac accaaaaaag gaagcatcac caaaatagga
     2881 atagccatca agacatagaa ccaaacccgc caaagcgccc aaaaaataat ttttattcct
     2941 ctcatagttt tcaaaagtaa gtaaaagtat gtatgtctga ctgcaaattg aacaaagttt
     3001 tttcgcaaaa tcgaaaaatg aagtctttga aattagtaaa aacctcaaaa tttttaaaaa
     3061 ataactacat aagaaaaaca aaaaaagacc gcaaattcgc aaattaaagc attataatta
     3121 ctttaataat ttgcgaattt gcggtaaaaa aataaaccaa actaagtgaa ctttgcgaaa
     3181 aactttgcga actttgcggt caagaaaaaa aatataatgg caaaaatact cactggcgtt
     3241 caaagcaccg gaacaccaca cttaggaaac ttactgggcg caattattcc cgctatcgaa
     3301 ttatccaaaa atcccgcaaa cgaatccttt cttttcatcg ccgatttgca ttcgataacc
     3361 caaataaaag atggtaaaac attaagagcc aatacatata gcaccgctgc tgcttggttg
     3421 gcttgtggtt tggatgtaaa taaagtagtt ttttaccgtc aatccgatgt ggcacaaaca
     3481 gcggaattgt cttggtattt gagttgtttt tttccatttc aaaggttgac cttggcgcac
     3541 tctttcaaag acaaatccga tagattagaa gacgttaacg caggattatt ttcgtatcca
     3601 atgttgatgg ctgctgatat tttgttgtat gatgcgcagt ttgttcccgt tggaaaagac
     3661 caattgcaac atttggaaat cacacgtgat gtggcttccc gtttcaatca tcaaatgggt
     3721 gaaacattcg tacttccaga agctaaaatt caagaagaca gtatgctaat tcctggtacc
     3781 aatggtggaa aaatgagtaa atctgccaat aatatcatca atattttctt ggatgacaaa
     3841 gccttgcgca aacaaataat gagcatcgaa acggacagca caccgcttga agctcctaaa
     3901 aatccagata cttgcaattg ttttgctatt tattcattat tggccaatga agaacaattg
     3961 gaacaaatga gagctaatta tttgggtggc aattacggtt acggtcacgc caaacaagct
     4021 ctgtttgaac taatcgtaga aaaattcaaa accgaaagag aaaaatacaa ttactacatc
     4081 aacaaccttg cagaagttga tgccttatta aaaattggtg ccgaaaaagc tggagttgtc
     4141 gccagtggcg ttttggcaag agtgagagag aaattggggt ttgagtaaaa aaataaccgc
     4201 agattcgcag attttttaaa ttgttattat aatctgcgaa tctgcgattt tatttaaatc
     4261 gcctcaaaca atttacccgg caagggtcga atcacgcctt tgagttccat atttagtagc
     4321 attcccgaga ttttataaat cgggaaatca catcgcaacg caataatatc caacaattct
     4381 ttaccgtttt ttagcaaata atcatacact ttttgttcgt cgttttcgag gactacaaaa
     4441 agttgctttt gaacaggttt agctttgctt tcaatgtccc aattcaggat gtaaaccaaa
     4501 tcggcagcac tggtcagcag gtttgctttt tgtgttttta tcaaattgtt gcaaccttga
     4561 ctgtatttgt cggtagttcg accaggaacg gcaaaaacat ctcgattgta atcgttggca
     4621 atattggcgg taatcaaaga acctcctcta tcggccgatt cgatgactat tgtagcttcg
     4681 gtcattcctg ccacgatgcg gtttctacga acaaaatttt ctttttcagg attagatgta
     4741 ctccaaaact ccgtcataaa accgccattt ttctccactt tggctacata ctttttgtga
     4801 tctttagggt aaatttgatt caaaccgtga gctacaacac caatggtttg caaatcatgt
     4861 tccatcgcca attgatgggc aaaaatatca acgccataag caaaaccact tacaattaca
     4921 ggatcaagcg gggccaaatc ttcaattaat tttcggcaaa attccattcc gtaggaagta
     4981 atttttcgag ttcccacaat gctgatgatt ttcttgtttt ttaggtcgat attgcctgaa
     5041 gtaaacaaca aaactggccc gtcgatgcaa tgtttgagtc ggtcgggata attttcatct
     5101 tgaaaataag ctacatcaat ttcgtttgat tttatgaatt ttagttcttg ttcggctttt
     5161 tcgaaaacgg ttttgtcttt aagatttttt aaaagcaccg aaccaattcc atcaatagaa
     5221 gctagttgag agcttttggt tttgaaaact tcgacggcat ttccgcaatg cgtaatcaat
     5281 tttttggcca caatatcgcc cactccttct acttgttgca atgccaataa atgaaataaa
     5341 tcctggtctg tcatactaat cgtttgaggc tgaaatgtat aaaaatttag ccggattgtt
     5401 aataaaaatt gtgaataaaa ttttgacaac tatattcaat gtataacttt gtaactatga
     5461 acatcgaact ctatatctcg cagcttttgt accgttacca atgtgtaacc gttcctggtt
     5521 ttggggcttt tttgactgaa atccagtcgg ctcaattaat tgaaagctcc cattccttct
     5581 ttcctccaaa gaaaatgatt tctttcaacg cccatctaaa aaataacgat ggattattgg
     5641 ctaatcatat tgcccaagcc gagaaaacgt cttatgaata tgccatcagc gcgattcaat
     5701 acgaggtttt caattggaaa aaagcactac aagaaaacgg attactttcc atcaaaaacg
     5761 tggggaattt ttcgttgaat gccgataaaa atttgatttt cactccttac gaccaaacca
     5821 attatttaac taattctttt ggtttgagtg cttttgtttc gccagttgta aaaagagaaa
     5881 tttttgaaca aaaaatcgaa gcacttattg aagaggaaaa agaagtaatt gctttcgaac
     5941 ccgaaattag aacttctaga ccttatttga aatatgctgc aattttaatt ctaggattgg
     6001 gtttaacagg tgctattggt tatcctgttt accaacaaca aattgaaaat caaacaatta
     6061 ttgttgaaaa atcggttcag aaacaagttc aaaataaaat tcaagaagct actttcttta
     6121 tcgaaaaccc gattccagcc gtaacgctta ctgttaaaga gaaaaaaatg tcttaccaca
     6181 ttatggctgg tgcattcaga gacgagaaaa acgcccaaaa gaaattcaaa gaattgtcta
     6241 acctaggata taaagcacga agaattccgc aaaacaaaca cggtttatac cccgttcttt
     6301 acggaagtta ccgcactttt gccgaagcta acaaagctaa agaggaaatc attaaaactg
     6361 acaatcctga tgcttgggtg ttgattgaat cactataatt taaaatcctg ttcgaaatga
     6421 acaggatttt tttgtgattt gtttattgct aatactccca aaaagagtaa aaaagatatt
     6481 aaattatttt attcagattc tataaaagtc tcccatctcc taaacagtcc ttcttcactg
     6541 atcttgtatg attgatgcaa tttggtaatt cttaaaccta tatcaatatc atttagacct
     6601 gcgtattcac ttgattctat taaagttgat ttcaatttta gccgcatatt ttctgggtta
     6661 actgcaatca tatccaaatc gaaaagagta tgtatatctg ttcttaacaa caaaccgttt
     6721 gaaagactaa aatctttttt taccgaatga ggtaatatat gtgcagcttc caacaattca
     6781 ggaatcttac aaccagtaac aatacaggtt cttttatata atttaagtaa cttttgacgg
     6841 aattttggct gccctctcct taccttaatc tgtgctaata catcttttat gaattcttct
     6901 ggcacatctg gttgattcaa ttcatcaaaa tcaagttctt cctcatctat ttccaaatca
     6961 gtttcctcag cagatatttc gaaattatcg ttttctactg tttccggaat ttctagagcg
     7021 gtaaaaggat aatataatcc atcttttaga cttactccat aggtttcaat cattagatcc
     7081 aataggtatt ttacctgaat ttccaaaagt ccataacggt tattaaaatc ttcggaaagc
     7141 ttttcaaaac caatcccatt tgaaatttta atgatttgtt tcaaatattt tccgtgtcca
     7201 cctttaactt ttggaaattc agtgttttga tagtctttct ctccaagacc ataaaaccca
     7261 ccttcaaatt ttacaaaata ttgcttacta tcctgttcca aaattgcttg tactgtaagt
     7321 aattttttgg gacgaggaaa taaattatta atatgttcga gaagttcgtg tatatgtatt
     7381 ggcgaatctt ttcttgacat atagatagat ataacatcat tcagtgttcc gcttcccatc
     7441 tgatctctca catctttaac attattcaaa ccaaagaggg cgcttttacc aacggaataa
     7501 aaaacttctc ctcttaataa agaagttctc aaaaccggca tagtccagtc cttttcggga
     7561 tgattttcaa ttactttctt aagaatatca tcagcataag caaaacctcc caattcacgt
     7621 aatacagttt caacaatttc aggctgtgta atgtgagaag tacgtttaat tattactttg
     7681 tcttctttga tttcagtttt aagtataagt tcttcagcaa taattgaatt ataactttct
     7741 atttcaaatt gagtaagtgg aatggtagcg taacccgata tatcaattac cttatcttgt
     7801 acaaatcgat cgttattttt atacatttcg atagccagac tatcgataac tcttttaatg
     7861 tcaaaacgtt cattctccag ttgtgtctga aggtataaat tgctccaaat attgccattt
     7921 gaatttcgct tattcttatt tgtgatttca ttaatatcgc caattaataa catatcgaaa
     7981 acaatcgata aggttttcgt ataaaaatga cttgtaaaat gcacctcttc agcttcattc
     8041 accatcttag tttgctctct tatttgcaaa aaattatgtg atgacacatc aaaaatattt
     8101 tcaagttttt cttttatcaa agtatccttc aaaaaccaaa aatcctgttc aaaattgtta
     8161 agggattcaa cctttaattg ccttaccctt tctctgtcaa gattattttt ttgggctatt
     8221 tcatccaatg tttttaatgc atttggtatg aaaactaaaa aagtaaagaa aatatcacta
     8281 actgcggggt tcttccaaag tttgttttgt tttatagcct cttctatcac acctaaatag
     8341 gaatgggtaa atttgtcttc ttccatatcc cataaataca accccattaa ctttgatatt
     8401 tctacttcat ttttagtatc aaaagtttct ttaataatat tccaggcttc tgttaatttc
     8461 tttctgtaat ccagtacttt atcatatttt acacctcgac cgaatccttt tgtattcaac
     8521 attttttgcc aatactcatc tgttgcaata attttgccaa tcaagttttt ccctgaaaag
     8581 tctccctgca ttatatcggc gaatgcgtta atattttgca attctggatt tttttctgat
     8641 tcgataaacc ttctatgaaa gccttttaag cattcgtata cgataatttc cataagtaaa
     8701 atttatgcta agtatattcc caattgttct ggctgtttgt tggctttaaa ggtcgtctta
     8761 taagtttgca gattaatgta aaattataat tcataaaatt taaatagctt attattggga
     8821 atgaaataat tttaaatact aaattttcta tgtgattact tcgtcccaag caatagataa
     8881 tgaagcaaat gtatcgtttc taattcaaac tcgccttaaa catccttaac tcttcccaag
     8941 aaaatttatc gccgtatttt tcttttaaag gtgaaatcga ttcttctttg taaccataaa
     9001 aagcctctgt caaatccttg attttatctt cgggcaaaac atcgttgacg cttattgttt
     9061 tggcgctaat caatttggtc aaatggctca aaatagtttc gggagtaaat tttctaatag
     9121 cagctatttc ggcaatagta ttgttttcga gccacaattc ataggtttcc tgaaccgtag
     9181 attttttggt ttctttcgtt tcttttttct ttttggtgta gcgatttaca tcggcttcat
     9241 cttcaatcaa cgtcacattg agttctttga attgcttttg aattgcttcg gttttgtggc
     9301 tgatgtattt tttaattaca tctgaagtta atttttcctt ggaaattgcc tcacctgcca
     9361 ccacggtttc gatcaacaat ttggctttca tcagtcgcaa aacggctttg acttgtagct
     9421 cttccaaaac cattaattcc tcataaaacg ctttagcttt ggaaacacgt ttgacttctt
     9481 cgaccttcca aagaatttca aaaaccaagt cgtccattgg tttgataaaa taattatacg
     9541 ctgccgtgaa acgctccgaa acgtgataca aatcgacggt ttctgtgtta aaaatcttgt
     9601 tgagttgaat aatgaatttt tgagatggat ccaaaagtcc gtcaatgata agtagttgtt
     9661 tggccgccca actagcttgt ttcgatttga ctgaaacatc actgctttcg ttgtaactaa
     9721 acttatgatt gcgccattct tgtgccaaat cgctccaatc aaaggtgttt atcaagtagt
     9781 tatggatgaa attcttggtt tcgaaatgca aggaattttt cagcacttct tccgaagctt
     9841 tgttcaacga ataatccatc acgtcttgat cgttcgaaat accattcatt cgcaatggag
     9901 aaagcaaaat taagccattt aacgaacgta aacgcgataa agctacataa gcttgtccgg
     9961 gaagaaaaac ttgcgaaaca tcgagcgctg ctttgtcaaa cgttaatcct tggcttttgt
    10021 gaaccgtaat tgcccaagcc aatttgatgg gataatgcac aaaagtacct aaaacctctt
    10081 cctctatttc cttggtcaat tcatctactt tgtagcgaat gttttcccat tcgtattttt
    10141 cgacttcgat ggtagtgttt tcttcgggaa aatgaaccaa aatctcgtct ttcgaaaggg
    10201 atttgataat gcccatttta ccattgaaat attttttatc aaatgacaaa tcgtttttca
    10261 caaacatcac ttgtgcgcct actttcaatt ttaattcctc atcaacagga taaattttat
    10321 ctggaaaatc acccacaatt acagctttgt aaacctgttc ttttccttct aaatcttcca
    10381 acgcttgggt gttcatcgca tcggctttgg cgttatgagt tgttaaagtg atgtaacctt
    10441 tgttggcttt caaatcgaaa tttggattta catattggtt caaagcctga acatcggcag
    10501 gagaaatttg attgttgcgc aaattgttca aaatcgaaat aaaaacttcg tctctttgtc
    10561 taaaaatttt atctaattca atatacaaag gcggattttg ctgaatgaca tgcgaatgaa
    10621 aaaagaattt tcctttgtaa taggtcttta aagtacgcca ttcttcgtct cgaattacgg
    10681 gtggcaattg caataaatcg ccaataaaca acacttgaac accaccaaat ggcgtcgttt
    10741 ttttgcgaac agtttgcatc ataaaattca ttgcgtcgag caaatcggca cgaagcatac
    10801 tcacttcgtc aataatcaac aattccatat tttgaataac cgatttcttc agtccactca
    10861 ttctgaaatg tctgcgcaaa gtagccttgg tttcaaactt ggtgctttcc gagaaatgag
    10921 gcgaagaatt gtcgggaata aaacctccaa aaggcagttg aaacatcgag tgaattgtca
    10981 ctccgccagc gttcaaagca gctattcctg tgggcgccac gacaacagta ttcttgtgtg
    11041 tggtttgaat aatttcccgc aaaagtgtgg ttttaccagt tccagctttt cccgtaagga
    11101 aaattgaacg atgcgtttga ttgataaatt tcagggtgta aacggctgct tcagagatag
    11161 tttgcatttg gcgtataaat taaatatggc tgttagaaga aatttttagc gacaagttta
    11221 caaattattt tgaaaacgaa gtattaaaat ctcaccaatt cgaatcaaaa ttagtttaat
    11281 gaaattaaaa aaaatatccg tgaatttcta gctaaaaaag aaaagctaaa atattgtttt
    11341 tgaagagata aaacaaaaaa tgcctccaaa aattgaaggc atttgataaa taaaatttta
    11401 caatattatt ttttagcagc tgatgctttg tatttttcgt tcaataattt aacaatttct
    11461 ttagtaatat cggctttatc ttcagcatat aaaatagttg cattagagtc gcctgttccg
    11521 tagatataag catatccttt ttcttttccg taccctttaa tgaatttttt tactccaaca
    11581 attacgctgt ccatttctac accaccttct tgttgtaact gttgtgctaa catttgttgt
    11641 gcttgagcca actgttgttc tcttctttgc aattcagctc cttttttttg agcccattct
    11701 tgaccattgg cttgtgcttg tgcttgaaaa ttttgagctt cttgtttgaa tctatttatt
    11761 tcagcttgaa gctgtcttcc tttctcttcg cctttggctt tatattttgc ttcaacatct
    11821 tttgcagcgg tgtattcttt cattaaaacc gaagtgtcca cataagctgt ttttacttct
    11881 tttacttttg tttctgattt ctctccgtta ttgcaagaaa caattgaaat tgcaattgca
    11941 agaattacta ctattttttt catttggtaa attttctgat ttttaagaat tggtaacaaa
    12001 aatataaaaa aataagtagt attatcaaaa acggatgaaa atgctacaac tatttacgat
    12061 ataagttttc gttattagat tgattgaatg tataaacaat cgctattaaa aacaattttt
    12121 ttagctcatt tctaatcgat tttctccaaa aagagaatat tcgtgttcgt caagacaaaa
    12181 aacattgctt aaaagcgctt aaaatgattt ttacttgttt tttaagacat aaatgtgtga
    12241 agaatactca aaattggact tcgctttcca attggattgc aatcctgtaa aaaacgcttt
    12301 aacgaaattc atttttcctg atttgtattt ttcagaaagc aaactcacat aaaacgaatc
    12361 aaatttcatt ggcaaaactt tttctagttt catttcttct ttttcaaaaa gcaattgtat
    12421 cgctgtttta gaaaaatgcc aaaaatgaat cggcacatca aaagctgccc aaaattttcc
    12481 gtaatgttgt gcatcgaaag atttgaaatt gggaaccgcc acaattaaag tccctgttgg
    12541 ttttaataag cgtttcaatt ctttgatttg tttgtctaaa tctgtaacgt gttccaaaac
    12601 gtgccacatc gaaatcacgt caaaagaatg actttctaat tcactggttt cttccacaaa
    12661 agaaatgcct ttgcttctgg caattccttt ggctcgatcg cttggctcta ctcctatggt
    12721 ttgccaaccg tcatttttgg caaccaacaa aaaatctcct gttcctgctc caatatctaa
    12781 tagtctccct ttgttggatt gttgcgaatt gattaaattg agcttattct ttaaagcaat
    12841 accttttata aaatgataga gtttttcaaa caaagaacgc ttattgtctg tgtgggaaat
    12901 ataatcttca ctttcgtagt atttgcccaa attctccaaa ctcggttgcg gatgtgtaat
    12961 cagcatatcc aaagtttcgt cataatacaa atcgaaagtc tcttgcgaaa ccgaataatc
    13021 ttttacagta aggaaatgtt ttttgtttga aatgttcatt tttagaaata ggtgttgggt
    13081 acttggtgtt aggttttagt ttgaaaatat ttgaaggttt cacgtggaac aaattagtaa
    13141 gcagtatcca gctgcaaact gaacactgaa tactgaacac tttttatctt cccatatgaa
    13201 tcaaaagcac agaaacatcg cttggcgaca ctccgcttat tctggatgct tgcgaaatag
    13261 ttacaggtct aatcttgctt aatttctgtc tcgcttcaat cgacattgat ttgattttgc
    13321 tgtaatcaaa gttttcagga atttttacat cttcgagtct tgataattta ttggcgttgt
    13381 ttctttcctt ttcgatataa cctgaatatt tcacttgaat ttcggcttgt tccaaaattt
    13441 cttgatccaa attgttttcg gcaacataag tggctacttt ttcgaatttg attatatcct
    13501 ccaaatcgat ttgcggacga gaaaacactt taaacatttt atcgccttgg gtaatcaagg
    13561 cagtatcttt tgattccaag attggatttg cttcagcaac ggttacactc gtttctttga
    13621 aaaaagctac catcttttct gactcattca atttgtgttc cattcgatgc aaacgctttt
    13681 cggaagccaa tcctatttca taagacattg gcgttaatct gaaatcggca ttatcttgac
    13741 gaagtaaagt tctgaattcg gcacgagaag taaacattct gtatggttct tctgttcctt
    13801 ttgtaattaa atcatcaatc aaaacgccaa tataagcttc atctcttttt agagtaaaag
    13861 gtgctttttc ctgtgctttt aaagcagcat taattcccgc catcaaacct tgagaagctg
    13921 cttcttcata tccagtagtt ccgtttattt gtccagcgaa atacaaacct tcaaccaatt
    13981 tagtttctaa agtatgtttc aattgggtag gcggaaaata atcgtattcg atagcataac
    14041 ctggacggaa gaatttcacg ttttcaaaac caacaaccga acgcagcgct ttaaattgaa
    14101 tatcctctgg aagcgatgta gaaaaaccat ttacatacac ctcgcaagta ttccaacctt
    14161 cgggttcaac aaacaattgg tgtctttctt tatccgcaaa acgattaatc ttatcttcga
    14221 tagacggaca atatcttggg ccaagcgatt tgattcgacc gttgaacatt ggcgaacgat
    14281 cgaaaccttc tcttaaaaca ttgtgcactt ccaaagaagt gtaggtcata taacaagacc
    14341 tttgatgaat caaaggtttc gtttcgtcag aatacgaaaa cttgtctggt ttggcatctc
    14401 ctttttcctc attcatttta gaataatcca aagaacgtcc atcgactctt ggtggtgttc
    14461 ctgttttcat tctaccagat tcaaaaccag cattgaccaa atcttcggta attccataag
    14521 cagcactttc tcctgctcta cctccaccaa attgtttttc gccaatatga attaatccat
    14581 tcaaaaaagt tccgtttgtc agcaccaccg tttttgatct gatttcaatc ccaagcgagg
    14641 ttttgattcc ttttacttta ccatattcaa tgagcaatcc tttgaccatt tcttggtaaa
    14701 aatcaagatt gggtgttcct tcgagcatca atcgccattc ttctgaaaaa cgcattcgat
    14761 cactttgaac ccttggcgac cacattgcag ggcccttgga cttgttcagc atcttgaact
    14821 gaatggcagt tttatcggaa acaattccag aatatccacc taacgcatca atttcacgaa
    14881 cgatttgtcc tttggcgatt ccgcccatag cgggattgca agacatctgt gcaatgtttt
    14941 gcaaactcat tgtaaccaac aaagttttcg atcccaaatt ggcagccgat gccgcagcct
    15001 cgcaaccagc gtgacccgca cctacaacaa ttacatcata ctcttctaaa aacattcttt
    15061 tattgatttt tgatttttag atttcagatt actttctaaa atctaaatct ttaatttaat
    15121 atacttgttc cacgtgaaac acatttcatt ttagatcgtt tctaaaacta aattttaact
    15181 taatcctaat ttgtttcacg tgaaacaaat atgcattctg aaatctgtat gcagaaatta
    15241 cgcctactgt tccacgtgaa acatagcgat tttttcgtct tctttagtgc gcatcaaaag
    15301 agcatccgct tccgttttgt ctttgtatcc acagtaatgt aatatcccgt gaaccataac
    15361 acgcttcaat tcttcttcaa aagaaacatt gaaatcaata gcgttgtctt ttactctttc
    15421 aacagaaata aacatatcgc cacttatttc attccccatc gtataatcaa aactaatgat
    15481 gtcagtaagg gtgtcgtggc ttaaatgctc caagttgatt ttgtgtaaat agtcgtcatc
    15541 gcagaagatg taatttatct cgccttctgt tttattttcg gatgtgattg ctttggaaat
    15601 ccaattagca atagcttctt cgttgtctaa aacgaactcg gtttcgtaat taaagtcgat
    15661 cattttttat tgaaatattc ttgaactctt tgattaaaat tcgtgcgcaa aggtagggat
    15721 tgtctattta aaatctctac agagtttaaa taatctaaca aagccgatgg caagggtttc
    15781 gattggtttg taaattcctt tttattcgtt tccgcttggc gtttgttttc ttgtccttgt
    15841 tgctgaatgg cggtgtttag tttcagtaat tcttgtttta cattaaggat gcgttgcagg
    15901 ttttcattct taaaaccttt gtttaataat tgcttttcta tctgcttcat ttgatccaaa
    15961 gcattctgtc cgttaccgcc aagaccacgt ttgttcaact cattttgcaa ggcttcacgc
    16021 aattgttttt gttctttgta aatttccata atggcttgcg catcgccttc gccatcttct
    16081 ccgttctcgc cttccgaacc actacctttt cctgattttc ctttttcacc cttttcgcct
    16141 tgcttgccct gaccgggttt gtcgcctggc ttctgccctt tcttcattcc gtctttcatt
    16201 ttctcggcaa gtccttcttg tttcttgata atatctggca attgcattcc gtcgccgtcg
    16261 cctttttttg gtttgcctgc acccatactt cccgacaatt gcatttgcat attgtttaaa
    16321 gcatcggata aaaagtccgc taatttgttt gccgaagcca ccgtatattg ctgatgcgaa
    16381 actccttttg caatttgagc atcggctaaa gtctctaatg atttgtctaa attataaagt
    16441 gcattaccca cttccttggt tatattttct tcaattttag ggttgcgcaa agacatcgca
    16501 aacaaactat catccacgtg tttgaactgt aatttcaaat cttgttggtt ttttaatgct
    16561 ttattaaacg caggagaacc tactttatag cctttgaatt tgcccatcaa atcttcttgc
    16621 gaaaaagaga aagccaaaag attatccaga atctgacgca acattgaaat atcttcttcg
    16681 agttcttctt tctctccgct ttccatttcc tgttccattt tttgggacat ttgcttcatc
    16741 tttttggcag cacttttctg ttttggcttg gcagatgact tactgttttt ctgtaattca
    16801 tccgaagctt ttttcaaatc ttcatcaatg cttttttcct gtgcaggatc atctagaatg
    16861 tacaacggct tttttaattc catattttca ttatccaagt cttttaaatc ttcctgaatt
    16921 ttatcgaatt ctttgttgat ttcgtcttgg ttttctttag tgttttcttt ttctctgttt
    16981 gacaaatctt cttgcttgtc ggcgagtttg tccaatttat cttttagttg ttcagctttt
    17041 ttctgaacat aatatttctt ggttagctcc accaactgct ccaaactctt ggtttgattt
    17101 ttggccgttt gtttgaactt atccattttc tccatcaagt cttcttgctt gattttgtcg
    17161 ttcaactctt ttaactcatc taaaagtttc ttatttttct ctaaatcctt atcgacgttt
    17221 tctaaccttt tttgcaattc ttctttaaac tcgtctttct ttccggtttt ggatttatct
    17281 aaattgtctt tcattttttc ggcaaaatcc ttcatcattt tatcctgctg atgctgacgc
    17341 ttgataaaat cattaatttt ttgctgatct ttaaattcga gattgtcttt ttcttttcct
    17401 gctttttgca atttatccat ttcagaaatt tgcttgtctt ggttttttaa tgacttttcc
    17461 aacccattaa tactttcatt ttgttgttga aaatcctgat cttctttctc ttcatccgta
    17521 gcaattcgat tcgagaaaac agaagattta gtccttttga aattatgaat agcatcgtta
    17581 tcaaaaacct caaaatagta ttcatacgac actccctgct ctacaggaag cgaacttgga
    17641 aaagcaaaaa caaactgatc atacagattc gacttaactg caattgttcc acgtttggcc
    17701 gattctggtt tgtttttagg ataataaact atctgaagtt tagacaatcc ataatcatct
    17761 gaaatttgcc ctaaaacata gcttttatcc acttttaaac tatcgggagc gttgttgaca
    17821 ttgatggtcg gaaattgatc tttgataact gaaatctgat aattcagttt ttcatagttt
    17881 tttactttag aatttgaagt caggatttga tattctgtat tttcaacgat ttgctttgat
    17941 aaagaaaaat tgttttcagt tttagcaaat gaagcattgg aagaagcatt tttccatacc
    18001 acattttgag ttgccaaagc attaattctc caagttacac gagttccttc gggaagaatg
    18061 gcatttccat ttcctttgat aatttctggc tttttgttta gataagcagg gaaatttaaa
    18121 agcatttcga aattagcaat cgatggaaca ctcaccacat tcaattcata atcggatgaa
    18181 gaaatggtat tcgcctccac gtggaacaaa atattggaag acggtttttc aattcggtat
    18241 tgaaattctc ccggcttggt tgattccata aaataacttt cgtcgccaat aaaaatcatt
    18301 gcattttcag gaacaatttt cccttgagtt ttgatgcgaa cgatgaaatc ttgattttgt
    18361 tccgtttgta aattactatt caataacaca aattcaaaag gagctggtgg caaaaattgt
    18421 tcacggaaat gcactaccct attcaaactt tgagagatga ttgtactgtt tcccgaaaca
    18481 aagaagaaag caaaaaacat aataggcaaa atcgccaaag gcaaatattt tctgttcgta
    18541 ttaaaattaa tggcattacc aaaatgaatt ggttttagtg cgttggcttt ttgttctatt
    18601 gaagccaata ataattccga ttgattttgg tcatttgata attggaggaa attcgtcaat
    18661 ttatcattta cttccgtgaa atgatttcca atgatagcgg atgcttcttt gtaatcaatt
    18721 cctttttgaa gcttgaacaa tttgaaaatg ggaaacaaaa taaacccgaa caaaaggaaa
    18781 atctcaacac ctataaatgt ccaaaacaaa agggttcttc ccacaggctt taaccaaaga
    18841 aaatattcta caaaaacggt aaacaggaaa tacagtaatc ctaacccaat aaagaaaatc
    18901 attccccgaa tgagttcgtt ggtgtaaaac ttcctgatga aaagttccag cttttgataa
    18961 atgaaatttg attgctccaa aatgtgtgac ttgtttttat aaccgataaa agtaactatt
    19021 tttcaattct gtttcagtta aaaattaatt aaaggtaaat tcatcgacta attctctaaa
    19081 aacttccctc ttccctcttc tgacttccaa cttctaatta tctttgcacc aaaatttata
    19141 aaaaatgtca agaccaattc gtgtgcgttt tgcaccaagt ccaacaggac ctttacatat
    19201 tggcggtgta cgtactgccc tattcaatta tttgtttgct aaaaaaaaca agggtgtttt
    19261 ctttcttcga attgaagata ccgatcaaac tcgttttgta cccggtgccg aagcctatat
    19321 tatggaagct ttggaatggt tgggaattgc gccagacgaa accatcggga aaaacgaaaa
    19381 atttggtcct tacagacaaa gcgatcgtaa ggatttgtac gtacaatatg cgcaacaatt
    19441 aattgattcg gattgggcat attatgcttt tgatactcca gaatccttgg attttcatag
    19501 aaaacaacac gaagccgaag gaaaaacatt tatttacaac catcacaatc gagagaaatt
    19561 agatacttcc ttggtaattt ctgctgagga aacagccaaa agaattgcca atggcgaaca
    19621 ttatgtaatc cgttttaaaa ctcctgtcga tgaaactttg catttaaaag acattattcg
    19681 tggcgaagta aaatttgaaa ccaatctttt ggacgacaaa gttttgttca aaagtgacgg
    19741 aatgccaacc tatcatttgg ccaatattgt ggatgactat ttaatggaaa ctactcacgt
    19801 aattcgtggc gaagaatggt tgccatcaat gcctttgcac gttttattat acaaagcatt
    19861 tgaatgggag gctccagaat ttgctcattt gcctttgata ttaaaaccag ttggaaacgg
    19921 aaaattatcc aaacgtgacg gtgataaatt aggttttcct gtatttccat tggaatggaa
    19981 aacagaagaa ggcgtttcat caggttatag agaaaaagga tttttccctg aaaccgtggt
    20041 caacttcttg gctttattgg gttggaatga cggaacggaa caagaattgt tttcgttgga
    20101 agaattagtg gagaaatttg atttaagtag agttcacaaa gcaggagcta aatttgatcc
    20161 cgaaaaaaac aaatggttca atcatcagta tttaatcaaa caaaatgatg ccgatttggc
    20221 gaaaagcttc gcttctattt tagaagaaaa aggcatctcg actgcgctcg atttgacaaa
    20281 aatagtttct ctaattaaag aaagagcaca tttcgtttcg gaattttggg atttgagcga
    20341 ttactttttt caagcaccaa ttacttatga cgaaaaagcg agaaagaatt ggaaagagga
    20401 aacgcctgca ttaatgcaag aattgatttc agtattggaa gaaataagtg attttacatc
    20461 tctaaatatt gaaaccatcg tcaaagactg gatgaccaaa aacgaaattg gaatgggaaa
    20521 agtaatgcag ccgtttcgtt tgagtctagt tggtgcccta aaaggtcctc acctatttga
    20581 catcgtggaa ctcattggaa aagaagaaac aattcaaaga attgagaaag caatagctac
    20641 tttataaaac taaaaaagct cccaaatttg ggagcttttt ttataaataa ataatcaaat
    20701 tgccattcaa tattccagga tttcctttaa aattatagcc taaatttttg ttgttcaagt
    20761 ttcctagcat attgttaacc ccatattgat aagaaacatt caagcgaacg tggcgaaccc
    20821 ctgctgtaat tcctactact ggataaaaac taaatttaga aatatcaaca atatctttgg
    20881 ccaacaaagt agttccagaa atactattgt tttgtttgtc tttttcaaca cttaattttc
    20941 cattaatttg aactaaagga cctatttcta cactgagatg attttcgaca attttataac
    21001 taaacagaaa cgatatttgt acagaagata atttataatt ggtgtcttct gttataaaac
    21061 cactgttcgt actaaccgaa aaattgttct cactgaactg cattgcataa agtgcatccc
    21121 aattgtcata gtaatttcca cgcatagaaa gtccgccgtt ccaacctgtt ccgggtttgg
    21181 tttgaaaatt attggtgttt aaagtaaact gattgacacc aaaacttatt ccgattcgat
    21241 tggaatctct atactcatat tgagaaaaac aaatcgtcga aatcaaggtt aaaacagaag
    21301 taagaaaaat ctttttcata aatattgaat tattcaaaga caaacctaaa gtttttttgt
    21361 aatttaacgg tattttttaa cctaaattat attctatgag tcctgtatta attattattt
    21421 tagtctttgc actatttatt ttcttctcga gtttttttac cgtcaaacaa caaaccgcag
    21481 ttgttataga acgatttggt aagtttattg gcattcgaca atcgggtttg caactcaaat
    21541 tgcctgtaat tgataaagtg gctgggcgtg taaatcttaa aattcaacag ctagatgtta
    21601 ttattgaaac acaaaccaag gataatgtgt ttgtaaaaat gaaagtatct gtacaattca
    21661 aagtaattcc agaacacgtt tacgaagcat tttataaatt ggaatatcca cacgatcaaa
    21721 ttacggctta tgtttttgat gtggttcgag ccgaagtacc aaaattgata cttgacgatg
    21781 tgtttatccg aaaagatgat attgccattg ctgtaaaaag agaattgaac gaagcaatga
    21841 ctacttatgg atacgacatt atcaatacgt tggttaccga tattgacccc gatattcaag
    21901 taaaaaacgc tatgaatcgc atcaatgctg ccgagagaga aaaaacagca gcaatgtttg
    21961 agtccgaagc gcaaagaatt agaattgtgg ctaaagccaa agccgaagcc gaaagtaaaa
    22021 aattgcaagg tcaaggtatt gccgatcaac gtagagaaat tgcccgtgga ttggttgaaa
    22081 gtgtggaagt tttgaacaat gtgggcatca attcgcaaga agcatcagca ttgattgtaa
    22141 tcacgcaaca ttatgataca ctgcaagcca ttggcgctga tacgaattcg aatttgattt
    22201 tattgcccaa ttctccacaa gccgccagcg atatgctcaa cggtatggtg actagtttta
    22261 cagcttcgaa tattattgga gaacaaatga aaaaacaacc caaacgagaa aaatctaaaa
    22321 atcacacctc gacagattac aatccgtcag atacttcaaa tccagctgaa ccagcttaaa
    22381 aacaaagtgt ttttaaatga aaaaagaggc ttaatggcct ctttttcttt ttgttatcca
    22441 attgattaaa attggaatgg caatattaat tatagttccg atattgttag aaacaattga
    22501 tttataatca cctaaaaaac tctttactat gtttttgggt aataaacttt cttttgtctt
    22561 ttcgacgttt aaaacgactc ttttcaagtt gagttctctt tctattttca agatttttaa
    22621 ttccaactca atttgtgcgt aggatgaata ttttttgttt tccatacttt agtcgttaaa
    22681 aaagatttct gaaaatttct ccaagatggg tccttccacc attttatctt taattaaaaa
    22741 taaaagtcct gagaaaacta aataaattcc acctactatt aaaaatccta aggcataact
    22801 atccaaggca ttcccgattg cgatggctcc tgctatagaa caaaacaaca aaaccatcat
    22861 caaagcaatg gcaattaaaa caaacttaaa aatcatcgtg gtagatttca ttgccacctt
    22921 aaaaccccat aacttataat aagctatact gttttcgata taagcttgcg cttctttttg
    22981 aatgtcttcg gtatgttctt ttagttcttc aaaagccata aattagtgtt taagaaaatt
    23041 aattttcgtg attagctaat tgtttttttt gaagtttggc gttttgttct ttcaaatcgg
    23101 ctaatttagt ttccaaaaaa gaaataacgt cttcagtttt ataactcata ttcgaaacca
    23161 tttcttcgta agtttcttcc atattgtgtt ttgatccttc aaactttttt ctcaattgat
    23221 gcgttacatc ttcaaattta tgttttaaat tgtctttagc ttcatcaaaa ccatctttaa
    23281 ttttttctct ggttttcgaa cctttatctg gagcaaataa aattccaact cctgctccaa
    23341 tcgctgcacc taataaaagt gccaatactg aatttgtgtt atttgacatc gtttttagaa
    23401 tttaaattaa tactgtccta aagttaatct atttattaac acagcaatgt taacaagccg
    23461 ttaaattgaa ctaaaactta aaataaagcg tttttttgaa tcaacttttc attaacaact
    23521 acaaagatgt aaaactaaaa taattgcttt aaaattaatt tatgaaagtc aattttaatc
    23581 aattaaattg atataatttt aaaaatctat caaaaaaata aataataaaa aaagcgcttt
    23641 tagaagcgct tttgatagtt attctttatt taaaatttgg tcgataaatt ccgtttctct
    23701 ttcggtggta tttttcggag gattggattt taattttaaa atttcttctt taaaggaatt
    23761 tacaaaatca gaatcgtaac ctaaatgttg taaattcaaa agtgttctcg aacggacaaa
    23821 tgttgattca cttcttaaag acatcaaata caatttagtt aaatcttcat tatcaaaatc
    23881 ggatgatttc aaactggaat atttcaaaat taaattggca aacaacaagg aacttttgtt
    23941 attgttgatg ttctttttga atgaattttg aagtaaacta taattcgaaa ttccggccaa
    24001 ggtctcccct attgcttttc tgatagcgat acgttcttct ctggtagcaa ctttaaaata
    24061 tttattgatg tctttcagtg aattattgat actattttcc tctttttttc ctttttcttc
    24121 ccaagcatct tttagtccaa cggttttgtt aaagactaat ttagaagcag ttgaatcttt
    24181 atcaacatta aaataaccca aacgttggaa ctggaattta tctccaggtt tcactataga
    24241 aagacttggt tctacaaatc cttttacgat ttgcaaagag tttgaattca taaaatcaag
    24301 gaaatttttc tccttatgac tgtctggtgc ttcgtctata aaaagtctat catacaaacg
    24361 aacttccgct tcaacagcat gctttatgga aacccaatgc aaggttccgg ctacttttct
    24421 tttagaggct tcactaccgc ttccactcaa ggaatcggtg tcataagtga cgtgaatttc
    24481 tgtaatattt cctgctgcat ctttcacaac actttctcct ttaatgatat aggcgttttt
    24541 taaacgtact tcatttccta aagacaaacg gaaaaatttg gctggagcca cttctaaaaa
    24601 gtcttctctt tctatgtata attctctcga aaatggtact tttctaaaac ctgcattctc
    24661 atcttcttga ttgttttcgg catctaaaag ttcttctttt tcttctggat aattggtaat
    24721 aaccactttt atcggatcta aaacagccat aactctagga gctgttttgt ttaaatcttc
    24781 tctcaaacaa aattccaaaa gcgaaacatc gatgatattt tcacgtttgg ccacaccaat
    24841 aacatcgcaa aatttacgaa tcgaagccgg cgtataacct cttcttctca aacctgaaat
    24901 agttggcatt cttggatcat cccagccgtt tactatattt tcttgaacca attgcaatag
    24961 ttttcgcttg ctcattacgg tgtaattcaa gttcaaacgg gcaaattcgt attggttggg
    25021 tctaactaaa gtatcgtcat aaatttggtc taaaaaccaa ttgtataatt ccctgtgcat
    25081 tacaaattcc aaggtgcaaa tggaatgcga aatttgttcg atataatcac tttcgccgtg
    25141 ggcataatca tacattggat agattttcca atcgttacct gttctgtgat gatgacggtg
    25201 taaaattctg tacatcaaag gatcacgcat caacatattg gtggaagcca tatcgatttt
    25261 ggcacgcaaa acgtgacttc cttcgggaaa atcgccgttt ttcattcctt caaacaaagt
    25321 caaattttct tcaaccgaac gatttctata tggtccatcc actcctggtt gagttggcgt
    25381 tcctttttga gccgccatat cttcagaaga ttggctatca acataagctt tccctttttt
    25441 aatcatcaaa atggcccaat cgtacaactg ctgaaaataa tcggaagcat aacgttcctc
    25501 ggcccaattg aagcccaacc attgcaaatc ttccttgatg gcatctacat attcctgctc
    25561 ttctttggcc ggattggtgt cgtcaaaacg caaattgact ggcgcattgt actttagacc
    25621 caaaccaaaa ttcaaacaaa tagatttggc gtggccaatg tgtaaatatc cattaggttc
    25681 tggtggaaaa cggaaacgta aattggattg gggaaaaccg cttgctaagc tgtcttctat
    25741 aatttgttct ataaaatgaa gggatttttc ttcggttgac ataattttct aaattgaaat
    25801 gcaaatgtaa aaaaatagat gcagataata gacttggaga taatagactt tcaattaaat
    25861 ttattcctat ttttgtataa atattgaacg ttatgagaca agttactgtt acaattccag
    25921 atgaatacta tgataatttg atagaatttt tgaagcctat tcctgatgct gttgttgata
    25981 atagccaaga agaatacaat cgatcaatag aaaaaatggt tttagaacgt gtaaaaaatg
    26041 ctaaaccaga agattatgtc gatgctagag aatctgtaca aaaattgaaa ctcaaatatg
    26101 gtttttaaag tacaacaaca aattcgtgca gaattagaac ttgacgaaat tatttattat
    26161 tatgaaaata ttcaagttgg tttgggaaat agatttttaa ttgactatga aaatctaatt
    26221 gatatattag aaacatatcc tttttttgag caaaaatata atattataag aaaactacct
    26281 ttcaaaaaat ttccgtacac cattcatttt caaatagatg aatttgagaa aatagtttat
    26341 attgaatcta tatcttgtga ttatcaagat ccaaatacta caagaataaa attataaaaa
    26401 tgggcatcat aaaactaaaa aacatccgta cttattccta ccacggttgt ttagttgaag
    26461 aaggaaaaat tggttcagat tattcggttg acttagaagt aaaaaccgat ttgcgtaaat
    26521 cagctctttc ggataatctg gaagatacag ttgattatgt gcttttgaac cgtattgtcg
    26581 aagaagaaat ggctataaga tcgcaattat tagaacacgt tgctcaccga attatcaaaa
    26641 gaatttttga tgaagtggca gctatttcga gaattacctt ggcagtttca aaattgaatc
    26701 ctcctattgg cggtgatgtt gaagcagtta ccatagaaat ggaagagtat cgaaattaag
    26761 ctaattatga atttttaaat ctttgtaatt attgctttcc ttttttaact tttttagtat
    26821 atttgcacac caataaataa ttggcgtcgt ggccgagcgg ctaggctggg ctctgcaaaa
    26881 gctcctactc cggttcgaat ccggacgatg cctcaagaag agatacagaa atgtgtctct
    26941 tttttttatg acatttttag gaaaaatatg gttcaaatta ggaacatttt ctaaaacatt
    27001 caaggaaact ttatccaacg tggataattt caaaaaaggt tagctaatgt aaattattca
    27061 aattcatatc tgaatgttga atattctcca ttatatactt cagttttata atcggtttta
    27121 tttttctttt caagttctat ttttagattt tcatatttaa aattgtaatg ccttttattc
    27181 ataaatccta cagtcctaat tgtgggatgt tctaaaattt gattaagatt accgtctaaa
    27241 atattggtgt caacaaaacg aaaatcaata aggtttggga atttatttaa aaaatcaagg
    27301 ttttcaatag aatcgcaaga atttaaacac aaaactttca acttttttaa cttcaataag
    27361 ctttcggtaa atttaaactt ctttgattga ttgatatgta aaaattctat tgtatcgctt
    27421 aatttatcaa tttcattgtc gttttcaagt tttaagcaat taaatagttc aagtcgtttc
    27481 aaatttttaa attttccaat tccactaaag tctttgatgt tagcataata tagttctaaa
    27541 tgcaacattt tttcagattc attcaaatta tcaaatgaat ttattttttg ctttaagccc
    27601 caaatgattc cgtattcaga attttcaaaa ttaaaacact cttttttgtt tttattatct
    27661 ctaaaatcct taataaagat tttatctttt tctatttgtt cgtaccaaat tgtattaaat
    27721 ctccaatcca ttgttgttgt tttaatgtat caaaatatta cagcaattaa cttgaaaaca
    27781 gaagttaata gtttttttaa acaattactt atcaatcttc tccaaaacat ctttctttac
    27841 atctggaaac caaccttgaa caattaaagc cactccaaaa atcattaaaa caatactgat
    27901 tcctttcttt attttaagga tattggttgg tgtcattttg gtttttaact gcttggccaa
    27961 tactattttg atgcaatcaa ccaataaata agatacaata acagttgtga aaaaagttaa
    28021 cattctagag ttttgcattt ctaatttggg tcctactgaa ataattacag ccaaccaaaa
    28081 tcccaatact ccaatattga taatgttaag aaaaaagcct tttataaaca aacctaagta
    28141 atcttttttg ataatttcgt
//
LOCUS       DAOSNN010000007        27058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_1783_length_27058_cov_5.369811, whole genome shotgun sequence.
ACCESSION   DAOSNN010000007 DAOSNN010000000
VERSION     DAOSNN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 27058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27058
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1783_length_27058_cov_5.369811"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1004)
                     /gene="accD"
                     /locus_tag="PLQ70_00720"
     CDS             complement(150..1004)
                     /gene="accD"
                     /locus_tag="PLQ70_00720"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQE33167.1"
                     /translation="MAWFKRTEKGITTATEDKMDIPKGLWYKSPTGKIIDAEELARNL
                     YVSPEDGFHVRIGSNEYFEILFDNNEFKELDAKMTSKDTLHFVDTKKYSDRLKDTISK
                     TKLNDAVRTAVGKSKGKDLVVCCMDFAFIGGSVGGVVGEKIVRGIDYSIKNNIPFVMI
                     SKSGGARMMEAAYSLMQLAKTSAKLAQLADAKIPYISLCTDPTTGGTTASYAMLGDIN
                     ISEPGALIGFAGPRVVKDTTGKDLPEGFQTAEFVLEHGFLDFITPRKELKDSINLYLD
                     LIQNQEVR"
     gene            complement(1102..2169)
                     /gene="fbaA"
                     /locus_tag="PLQ70_00725"
     CDS             complement(1102..2169)
                     /gene="fbaA"
                     /locus_tag="PLQ70_00725"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HQE33168.1"
                     /translation="MAHNIKPGVATGDQVQEIFNYAKEKGFALPAVNVTGSNTINGVL
                     ETAAKLNAPVIIQFSNGGAQFNAGKGLSNAGEKAAIAGAIAGAKHIHTLAEAYGATVI
                     LHTDHCAKKLLPWIDGLLDASEAHFAATGKSLFSSHMIDLSEEPIEENIEICKGYLSR
                     MSKMGMTLEIELGITGGEEDGVDNSDVDSSKLYTQPSEVSYAYEELSKVSPRFTIAAS
                     FGNVHGVYKPGNVKLTPKILKNSQDYVQEKFKTGPNPVDFVFHGGSGSTLEEIREGIS
                     YGVIKMNIDTDLQFALTEGIRDFMVNNIDYLKTQIGNPTGADAPNKKYYDPRKWLREG
                     EVTFNARLEQAFADLNNVNTL"
     gene            complement(2239..4785)
                     /locus_tag="PLQ70_00730"
     CDS             complement(2239..4785)
                     /locus_tag="PLQ70_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQE33169.1"
                     /translation="MKNISTKIATFIVIGIIISACDAEKRVPKGKQLLTKNEIVVNDK
                     STTSEDITNQLYQKPNSTFLGYHLRLNLFNLAKPNPDSAFQAKFTNNPKKYERMSKWL
                     SAKQVDRLGQSFWYHGFHNFLKETGEPPVIVDTARTQKSIARVKYYYFNKGFFDVKAA
                     YKLDSLSEKKAKIKYTITTGNPFLIDTVSTSIQTPVLDSLYQTKISNSFIKTGKQYNT
                     EDFTNEKERISTHLRNNGVYNFQPNYVTFDLDTLNHNKKVNVNLIIKDYSYQDKDTTR
                     TEPFKMYKISDVNIYTDYSATNNSAVFADSTTYNNFNLYSHSKLKYKPKAITDAVFIT
                     KGSLFADFRTVLTTRYLNNLKIFNYPTILYEVDKRDTTAQSLIAKVYLSPRKKYSLQT
                     TLDVTHSNIQDIGIAGSISETIRNVFNGAETLELSARGNIGSSKDLANPQSKFFNVSE
                     YGIDAKLNFPRIWFPFATEKIIPKSMIPSTLVSVGLSNQKNIGLDKQNVTGMLAYNWN
                     PKKNNTARFDIFNVQYVRNLNPENYYNVYESSYNELNNLAKTYNYTEHVDIDGNLLIE
                     EGTDGFTDQVLTKAITVSDTDYETVSSIDERKTRLTEDDFILSTSYSFSKTTKSDLKD
                     NTFYQFRTKIESAGSVLSLISNAANLPKNANGNYEIFNLEYSEYIKTEFDYIKYWDFS
                     KEKVLAVRSFFGIAIPYGNSKNIPFSRSYFAGGSNDNRAWESYRLGPGSTGATNDYNE
                     ANMKIALSAEFRFKILGSVKGAIFADAGNIWNVLDNITDKRATFDGIQDLKELALGTG
                     FGLRYDLNFFVVRFDLGFKTFNPANEMGKRWFYDYDFAHSVFNFGINYPF"
     gene            4823..5548
                     /locus_tag="PLQ70_00735"
     CDS             4823..5548
                     /locus_tag="PLQ70_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQE33170.1"
                     /translation="MLSKNQIKLITSLQQKKQRIASQLFFAEGIKVIHELLESKFELV
                     HLYTTQNDFQEVSNHKKVLIDDSELKKITALATANTCLAVFKIPSEKKIIDSGLILAL
                     DSVRDPGNLGTILRLCDWFGIDQLICSRETVDIYNPKVVQATMGSIARVNVNYIDLES
                     FIVQTKLPVFGTFMDGKNIYKENLPQEGIIIMGNEANGISPTLEKLIQNRLTIPRFGT
                     LQKTESLNVATATAIVLSEFRRG"
     gene            complement(5582..6295)
                     /locus_tag="PLQ70_00740"
     CDS             complement(5582..6295)
                     /locus_tag="PLQ70_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQE33171.1"
                     /translation="MKKIVVLILLALSTQGFAQNKLFGKDPIINLENFQKQKLYFGFY
                     LGFNSYDFKVDYKTVSEDIQVKKNTGFNVGLVADLRLQEYINLRFEPGLYYTKRDLGY
                     PSSYFESTPTYSDRLREVNSTYIHFPLLLKFSSLRTGNVRPYILGGLSATLNLSSNAK
                     SIDDNYEQIFRVKSWTTNYELGFGIDIFSEYFIFSPSIRGVFGLTDELIRDKNPDSPW
                     TGNIESLKSRAVFINFTFH"
     gene            complement(6297..7028)
                     /gene="ubiE"
                     /locus_tag="PLQ70_00745"
     CDS             complement(6297..7028)
                     /gene="ubiE"
                     /locus_tag="PLQ70_00745"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQE33172.1"
                     /translation="MSKNITPYKDSTLSKKEQVATMFDTISGNYDNLNRVISFGIDVK
                     WRKKVLQIVAKNNPKTILDIATGTGDLAILMAQTKAEKIIGLDISQGMLEVGKTKIQD
                     KNLSNRIEMVLADSENMPFEDNYFDAITVAFGVRNFENLEKGLTEILRVLKPNGTFVI
                     LETSVPEKTPYKQGYTFYSKNILPIIGKLFSKDNVAYGYLSESAAAFPYGEALNNILK
                     KIGFIDVVALPQTFGVATIYSASKK"
     gene            complement(7110..7595)
                     /locus_tag="PLQ70_00750"
     CDS             complement(7110..7595)
                     /locus_tag="PLQ70_00750"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQE33173.1"
                     /translation="MIILIAAVAENNALGKNNDLLWHLPKDFKRFKEITSGHHIIMGR
                     KTFESFPKPLPNRTHVIITRQKDYVHEGCIVVQDLESAIAVCPKNEDAFVIGGGEIYS
                     QSIHLADQLDITRVHHSFEADVYFPEINSKIWELTSEIFNHKDEKHLYDYTFQTFVRK
                     T"
     gene            complement(7608..7940)
                     /locus_tag="PLQ70_00755"
     CDS             complement(7608..7940)
                     /locus_tag="PLQ70_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HQE33174.1"
                     /translation="MEKEQHEQYEYARKRLKQKKGLYFHFVLLVLGSIFMFIANHYSI
                     FGFTANWAIWVITFWAFLFILHFIKVYITDRFMNKNWERDQIDKLVAKQQQKIVQLHN
                     QIEENSTK"
     gene            8181..8477
                     /locus_tag="PLQ70_00760"
     CDS             8181..8477
                     /locus_tag="PLQ70_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HQE33175.1"
                     /translation="MSIKKQFIKTKPVCKVTFSVEAKEATTASVVGDFNNWNSAEGEL
                     SKLKNGTFKGVFELPKDTTYEFKYLVDGVYVNEPEADYFIWNEFAGAENSVLEV"
     gene            complement(8539..8904)
                     /locus_tag="PLQ70_00765"
     CDS             complement(8539..8904)
                     /locus_tag="PLQ70_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HQE33176.1"
                     /translation="MIKICDLEVSQLFLFKEQSYTGRCNVVYKDHGVEFHELSDDQRN
                     AFMRDVATTAKAIAAAFNPTKINYGAYADTLSHLHMHIVPKYKDGYGFGGVFEMNPQK
                     TTLTDAEYAEVIEKIKAGL"
     gene            complement(9013..9837)
                     /locus_tag="PLQ70_00770"
     CDS             complement(9013..9837)
                     /locus_tag="PLQ70_00770"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQE33177.1"
                     /translation="MKQYLDLVKHVLENGNQKGDRTGTGTKSVFGYQMRFDLAEGFPI
                     VTTKKLHLKSIIHELLWFLKGDTNIKYLKDNGVKIWDAWADENGDLGPIYGHQWRNWN
                     SEEIDQIADLINELKANPNSRRMLVSAWNPSVLPDTSISFAKNVANNKAALPPCHAFF
                     QFYVADGKLSCQLYQRSADIFLGVPFNIASYALFTMMIAQVCDLEVGEFIHTFGDAHI
                     YNNHFDQLELQLSRKPKLLPKMILNPAIKNIFEFTYEDFTLENYEPHDAIKGSVAV"
     gene            complement(9937..10560)
                     /locus_tag="PLQ70_00775"
     CDS             complement(9937..10560)
                     /locus_tag="PLQ70_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HQE33178.1"
                     /translation="MSLVKLSIKGISYSQTQNGAYALILNEIDGERKLPIVIGAFEAQ
                     SIAIALEKEIKPPRPLTHDLFKNFADRFDIVVKQIIIHKLVDGVFYSSIICERDKIEE
                     IIDARTSDAIALALRFNAPIFTYKNILDKAGIFLKSNPSNPEKDAQEIDDVLSNPETF
                     KQKEESNESGGTYSKYTLEQLNDLLEQAVEHEDYEKAAKIRDEISKR"
     gene            complement(10724..11689)
                     /locus_tag="PLQ70_00780"
     CDS             complement(10724..11689)
                     /locus_tag="PLQ70_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HQE33179.1"
                     /translation="MSILIYAESAEGKFRKVAFELASYAKKVAESIGTTVTAITINTE
                     DVSELSKYGVDKVLKITNEKLSIFSAKAYADVIKQAAQKEGTKLVILSSTTDSIYLAP
                     LVAVSLEAGLASNVVELPISTSPFQVKRNAFSNKAFNITEISSEVKIIGLAKNSYGIF
                     ENTSNLSVEDFNPTIGEADFGIKVEKIEKVSGKVSIADADIVVSGGRGLKGPEHWGMI
                     EELAQVLGAATACSKPVSDLDWRPHSEHVGQTGKPVAANLYIAVGISGAIQHIAGINS
                     SKVKVVINTDPEAPFFKVADYGVIGDAFEVVPQLIEKLKVFKAQH"
     gene            complement(11764..12510)
                     /locus_tag="PLQ70_00785"
     CDS             complement(11764..12510)
                     /locus_tag="PLQ70_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HQE33180.1"
                     /translation="MKILVCISHVPDTTSKINFANGDSQFDTNGVQFVINPNDEYGLT
                     RAIWFQEQQGATITVVNVGNAETEPTLRKALAIGANDAIRINANPTDSFFVAKQIAEV
                     AKNGSYDIIIAGKESLDYNGGMVPGMIAGILGYNFLNSCTDLLVDGSNVKAIREIDGG
                     RETISTTLPIIIGGQKGLVEEKDLRIPNMRGIMTARTKTLTVVEPIDASINTKAVKFE
                     KPAPKSAVKLVAADNLDELINLLHNEAKVI"
     gene            12752..13729
                     /locus_tag="PLQ70_00790"
     CDS             12752..13729
                     /locus_tag="PLQ70_00790"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HQE33181.1"
                     /translation="MRTIQFREAICEAMSEEMRRDESIYLMGEEVAEYNGAYKASKGM
                     LAEFGEKRVIDTPIAELGFTGIAVGSAMNGCRPIVEYMTFNFCLVGIDQIINNAAKMR
                     QMTGGQFNVPIVFRGPTASAGQLGATHSQALENWFANTPGLKVVVPSNVYDAKGLLKS
                     AIRDNDPVIFMESEQMYGDKGEVPDGEYTIPIGVADIKRAGTDVTIVSFGKIIKEAYI
                     AADELAKEGISCEIIDLRTVRPMDNEAILTSVKKTNRLVILEEAWPFASISSEITYIV
                     QEQAFDFLDAPIQRITTADTPAPYSPVLLKEWLPNANDVVKAVKKVMNK"
     gene            13840..16326
                     /locus_tag="PLQ70_00795"
     CDS             13840..16326
                     /locus_tag="PLQ70_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HQE33182.1"
                     /translation="MLKNIILYLSFIVVFTSAVFSQTKVSGVVLDQLNKPIPFANVAF
                     KNSSEGVVCSEDGVFYIESSKDYKTIIITSVGYSDKEIDLEKPINYNFKIQLKEIETL
                     NEVVIFRGKTSKKNNPALDILRKIWERRRKNGLHLFNQYQMEKYEKIEFDMNTIDSTY
                     MKNKLFKGMEFIFNHVDTSKVTGKTYLPIFINEALTDVYGDNKLKKIKEKTKANKTSG
                     FNGNQQILAFVNDLYTDFDIYDNHLTFFDKSFTSPLSTTGIDVYNYVLRDSAYIDDKK
                     CYNIVFYPRRKNELTFKGDFWVSDTTFAIKKINMAATRSANINWVKDIYIEQEFEVMN
                     DSIFLMTKDYMMTDFALSKKEKSKGVYGKRTSLYRDHKFNIEKPEKFYKEEVNYLDKE
                     VYNKSDDYWEKNRFENLTKDEKGVYKMLDTLQTVKKFQQLYSLVEVLDSGYLHFGNLD
                     YGPIYSVIGQNSVEGLRLRTGVRTYTGPNDPWRLQAYVAYGFKDDKFKYGVTGKWMVE
                     PKKRVIISAGNRRDIEQIGASLTTTNDIVGRNYASSGLLTFGSNNKLTNVNLSNVQIE
                     MEPIKNLTFSTGVSYRTLLSASPEFSLDYYTDLSQTKIQSQVKQSEVNLQVEYTPNRV
                     PIAFGVERDNADSPFTTLLVTYSQGFKNLFSSDFNYKKLQLYYRQPIIIGPLGRSNIT
                     FETGKTFGYIPLGLMSVVPGNQSLFDISNTFSNLKYYEFVTDQYVTFKWDHDFQGRFF
                     ARIPFMRKLNWRENIGVKSVYGTVSNDNRTINASGLEYHAPDKVYWEYSAGIGNIFKF
                     FRVDFSWRGNYLNVPDANKFATKISFGFYF"
     gene            16328..16930
                     /locus_tag="PLQ70_00800"
     CDS             16328..16930
                     /locus_tag="PLQ70_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HQE33183.1"
                     /translation="MENAIQYLLEKEAIFKIIIEKYGLPTIPKRPQGFETLVLLILEQ
                     QVSIDSAKATFLKLKAEQPNFNPKSLFSFSDEAFRNIGVSRQKTSYIKALSHSIINNY
                     IDLESFATKSSEQVREELIKIKGIGHWTIDIYLMFSLQAPDIIPLGDIAVVNTIKELL
                     DIHDKKEMEVYVAKWSPYQSIATFLLWHYYLNKRNRKLLY"
     gene            17029..17556
                     /locus_tag="PLQ70_00805"
     CDS             17029..17556
                     /locus_tag="PLQ70_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HQE33184.1"
                     /translation="MAADKITTFDVLIEIPRGSRNKYEYDFKLKRIRFDRMLFSSMMY
                     PADYGFMPQTLALDGDPLDVLVLINEPTFPGCVMEVKPIGVFHMADDKGPDEKIICVP
                     VSDPIWNSLSDLSDINPHLLKEIEHFFQVYKDLENKVVDVGGWGDLSEALAIIDECTE
                     RFNQIENKPADLFNI"
     gene            17686..20289
                     /locus_tag="PLQ70_00810"
     CDS             17686..20289
                     /locus_tag="PLQ70_00810"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQE33185.1"
                     /translation="MNAIMIYVPMLMALLGLVFMFLKRAWVLKQDSGDGKMKEISEYI
                     HEGALAFLKAEYRLMTLFVIVASIVLAGITFLPNATTHLLIVVAFVFGAFFSALAGNM
                     GMKIATKTNVRTTQAARTSLPQALKVSFGGGTVMGLGVAGLAVLGLTGFFIILFNVYM
                     NGVWTSTEDMTVVLETLAGFSLGAESIALFARVGGGIYTKAADVGADIVGKVEAGIPE
                     DDPRNPATIADNVGDNVGDVAGMGADLFGSYVATVLAAMVLGNYVIKDMGGNIQDAFG
                     GIGPILLPMAIAGFGILFSVVGTMLVKINSNDAKEADVQKALNIGNWASILLTGLACY
                     FLVDYMLPSKMNMSFFGEGLKEIASIRVFYASMVGLVVGAIISSVTEYYTGLGTKPVL
                     AIVAKSSTGAGTNIIAGLSTGMMSTFPKVIVLAAAIWISYSFAGFYGVALAASAMMAT
                     TAMQLAIDAFGPISDNAGGIAEMSELPKEVRTRTDILDSVGNTTAATGKGFAIASAAM
                     TSLGLFAAYVTFTGIDGINIFKAPVLAMLFIGAMIPVVFSALVMNSVGKAAMDMVNEV
                     RRQFKDIPGIMEGTGKPEYAKCVDISTKAALNEMLLPGILTIGFPIGIVLLGKLVYMN
                     DANANSMVAEMLGGYMAGVTVSGVVWAIFQNNAEGAWDNAKKSFEAGVLVDGAMSFKG
                     SDAHKAAVTGDTVGDPFKDTSGPSMNILIKLTCLIGLVIAPILGNGSASSDDKMIKCK
                     MEMKMSCPMGKEAMPMGCDMSKCATMTKDECAKMCDEKGCSPEQKEMCLSHYDANGKF
                     VADKKENCCAKKTDCFDTSKYEKKNVKVEMTNNDGKAKAIVTTSENGNVNVQVFEGSL
                     EDVKAKVEALK"
     gene            complement(20370..20738)
                     /locus_tag="PLQ70_00815"
     CDS             complement(20370..20738)
                     /locus_tag="PLQ70_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQE33186.1"
                     /translation="MEIRPIKTEKDYNFALERVNTLFDAKPNTNEGDELDILVTLIEK
                     YEQIHYPIPEPDPIEAIKFMMEQNGLSNADLGVILNSRSRVTEIFKRKRALTIRQIRL
                     LNENLHIPASTLIKEYALEQ"
     gene            complement(20741..21076)
                     /locus_tag="PLQ70_00820"
     CDS             complement(20741..21076)
                     /locus_tag="PLQ70_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016649370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQE33187.1"
                     /translation="MTFNIKTIAVFEKQTKRLLKKYPSLKSELIQLVQELKENPEQGN
                     RIGKNCYKIRISIASKGKGKSGGARIITHIVISKDTVYLLSIYDKSDQETLSQKELDE
                     LLKYISDDL"
     gene            complement(21066..21275)
                     /locus_tag="PLQ70_00825"
     CDS             complement(21066..21275)
                     /locus_tag="PLQ70_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33188.1"
                     /translation="MKVLVDIPDNKADFGLEVLRNLAFVKKASPISGKKFVLIEEIKE
                     AVQELNLIKASKKQARNAEDFLNDL"
     gene            complement(21657..22271)
                     /locus_tag="PLQ70_00830"
     CDS             complement(21657..22271)
                     /locus_tag="PLQ70_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQE33189.1"
                     /translation="MHIAVAGNIGAGKTTLTRLLAKHFKWEPHFEDVVDNPYLDDFYH
                     QMERWSFNLQIYFLNSRFRQVLQIRESGKKIIQDRTIYEDAHIFAPNLHAMGLMTNRD
                     FQNYSSLFELMESMVEAPDMLIYLRSSIPNLVSQIHKRGREYENTISIDYLNRLNERY
                     EAWIQTYTKGKLLIIDVDNINFVDNPEDLGMIINKIDAELNGLF"
     gene            complement(22271..23062)
                     /locus_tag="PLQ70_00835"
     CDS             complement(22271..23062)
                     /locus_tag="PLQ70_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33190.1"
                     /translation="MKNILLIAFISVFTFSFGQDEKCIAIKQSSTTLNTDYFIGYDSF
                     GYYYSIKNNVLSKNNAKESFEYKNISLGKPTKIDIQNPLKIMLFYENFNTAILLDNQL
                     SETQKINFSENDIPIVANAAGIASQNRLWVYNSLTQKLGFFDYLKNTIQTLTPSLSGN
                     LKYYETSFTTFQWIDDQLNWYSCDVFGKITTLGKIADFDQIQIISNQEIVFLKDGKLF
                     LQDLKTNSIYTIENVEKSFKNFFYKDQILSIFTNQEITNYKITIP"
     gene            23374..24255
                     /locus_tag="PLQ70_00840"
     CDS             23374..24255
                     /locus_tag="PLQ70_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HQE33191.1"
                     /translation="MLKIIHYVTLFLAINFGLQAQEFQGMAIYQSKTSTEDFKARMAG
                     NKDITPEMQKNMEERMKKMFEKTFILNFDKTASIYKEEEKLEAPTQGGGGFRMMGSMM
                     GAGGTFYKNIKEKSYTVDKEFMGKEFLIKDSLPRLKWKMEGETRVIGGFNCFKATAVR
                     PVSKTDFQNLRPKKEDESAAKPKTEDKKTDFMSGLEMPKEVVVTAWYTPEIPVNQGPD
                     SYWGLPGLILEINDGKTVILCSKVVLNPKEKAEIKASTKGKLISQAEYDETVIKKMEE
                     FREMRSRGGNSGPPRMN"
     gene            24305..27007
                     /locus_tag="PLQ70_00845"
     CDS             24305..27007
                     /locus_tag="PLQ70_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQE33192.1"
                     /translation="MKNIFFAVLFCVSSFSFAQAVKLEGTISDTKSLPVEMANVMAVN
                     QATKGMDSYAITNDKGKYSLSLKPNTTYSIKISFLGMQSKEVEIVTTATNISKNITLE
                     DSAVELDGVEIIREMPVSIKGDTIVYNADSFKSGTERKLEDVLKKLPGVEVNADGEVE
                     VEGKKVTKLMVEGKDFFDGDTKLGVKNIPADAIDKIQVLRNYNENSILKSVENNQDNV
                     AMNIKLKSGKKNFWFGDLTAGIGVGMLDSRYLINPKLFYYSPKYSINLITNFNNIGEL
                     PLTIQDYMKLTGGFRGLSAKGGSSFNITSNDLGISLMRNNRAKEIETQFGAANFSYSV
                     NKAWTLSGFGILSSSVTETETLSQTNYLDPNSSEIQSVENKKELSNQKSNLGMFKLSS
                     SYKPSTNFQFDYDVLAKLSKQQEDAPLFREAIVNSMTTSEVINTKKEQKPISVNQNLS
                     LFYTMNEKNIFAVEMQHLYQEEDPFYNANLKTQPFNLVGYVSGQDRNNISQNRFVTTS
                     KLDAKVDYYYMITPKSNFNITLGNTYSYQNFNSSMYQILDNQDVNELDSQVNVNDVNY
                     RFNDAFLGLHYKILTGKFTFTPGVSLHSYNMTNTQLNTDYSQNFTRALPDLLAIYQIK
                     KAESLTYNFSYTNNFTDINQLSEGFVFSNYNSLRKGSRTLENATARQHSLRYFKYNMF
                     NLENISAFLNYSKRIDAIKTKAFFDGVNQSSVPYNSDFADETLSGSGSYGRSFLKNYK
                     ASVSANINWSLFNNIQNNVSSKKESFTQSYTLRTSTNYKKLPNLEVGYNIVINNYDSN
                     VFYTDKPFAKLDYYFLNAFSFVAEYEFYHYYNTDKTVNNQYDFLNASLIYQKKDSKFE
                     YKIGATNLLNTKSINDDSFSQFSTRTSQYTVQPRYLIFSLKYNI"
BASE COUNT         8751 a         4225 c         4780 g         9302 t
ORIGIN      
        1 gtaaacaatc ccgaagattt aggaatgatt atcaataaaa ttgacgcgga attgaatggt
       61 ttgttttagg tttatccaaa tttcaaaata aaaaaaggcg caaaacttaa aatttgcgcc
      121 tttccttttt tacatcgaga atacatttat tatctcactt cttgattttg aattaaatcc
      181 aaataaagat taattgaatc tttcaattct tttctgggag taataaaatc taaaaagccg
      241 tgttcaagaa caaattcagc ggtttgaaaa ccttctggta aatcttttcc ggtggtgtct
      301 tttacaactc taggtcctgc aaaaccaatc aaagcgccag gttcagagat gttaatatct
      361 ccaagcattg catatgaagc agttgttcct cctgttgttg ggtctgtaca caaagaaatg
      421 tatggaattt tggcatcggc tagttgagct aattttgcag aagtctttgc taattgcatc
      481 aacgaataag cagcttccat cattctagct ccacctgatt ttgaaatcat tacaaatgga
      541 atgttgtttt tgatagaata atctattcca cgaacaattt tttctcccac aactcctcca
      601 actgaaccac caataaaagc aaaatccatg caacaaacca ctaaatcttt tccttttgat
      661 tttccaacag ctgttcgaac tgcatcattc aatttggttt tagaaatcgt gtcttttagc
      721 ctgtcagaat attttttggt atctacaaaa tgcaaggtgt cttttgatgt cattttggca
      781 tccaattcct tgaactcatt gttgtcaaat aatatttcga aatattcatt acttccaatt
      841 cttacgtgaa aaccatcttc tggacttacg tatagattgc gagctaattc ttcggcatca
      901 attatttttc ctgtaggcga tttgtaccaa agcccttttg gaatgtccat tttgtcttcg
      961 gtagctgtag tgattccttt ttctgttctt ttaaaccaag ccattttttt agtattcagt
     1021 gttcagtatt cagtattcag tgttcagtaa aaaagcattc agtttttaat aatcagtgta
     1081 aaactgaaca ctgaacactt tttatagtgt atttacgtta ttcaaatcag caaaagcttg
     1141 ttctaatctt gcattgaatg taacttctcc ttcacgcaac cattttcttg gatcgtaata
     1201 ttttttgtta ggagcgtcgg caccagttgg atttccgatt tgagttttca aataatcgat
     1261 attgttaacc ataaagtcac gaattccttc tgttagagca aattgtaagt cagtatcaat
     1321 attcattttg ataacgccat agctaatacc ttctctaatt tcttcaagag tagaacctga
     1381 tccaccgtga aaaacgaaat caacaggatt tggtccagtt ttgaattttt cttgaacgta
     1441 atcttgagag tttttcaaga tttttggagt taattttacg tttcctggtt tgtaaacacc
     1501 gtgaacgttt ccaaaagaag cagcaattgt aaatcttggg ctcacttttg ataattcttc
     1561 gtaagcataa gatacttctg aaggttgagt gtataatttt gaactgtcaa catctgaatt
     1621 gtcaacccca tcttcttcac cgccagtgat tcctaattcg atttctaatg tcattcccat
     1681 tttgctcatt ctagacaaat atcctttaca gatttcgata ttttcttcga ttggttcttc
     1741 agataagtca atcatatgtg aagaaaataa agattttcct gttgctgcaa aatgtgcttc
     1801 agaagcatct aataatccgt caatccaagg taataatttt ttggcacagt ggtctgtgtg
     1861 taaaattaca gttgctccgt aggcttcggc taatgtatgt atgtgttttg ctccagcgat
     1921 tgctccagcg attgctgctt tttctccagc atttgaaagt ccttttccag cgttaaattg
     1981 cgctcctcca ttcgaaaatt gaataataac gggtgcattt aattttgctg ctgtttctaa
     2041 aactccgttt atcgtgtttg aaccagtaac gtttactgcc ggtaatgcaa aacctttttc
     2101 ttttgcatag ttaaaaattt cttgtacctg atctcctgtg gctacgcctg gttttatatt
     2161 atgtgccatt gtaaattgtt taagttgttt ttatattgtt gttgttttta cgtttgcaaa
     2221 aataagaatt aattagcatt agaatggata atttatacca aaattaaaga cagaatgtgc
     2281 gaaatcgtaa tcgtaaaacc atcgttttcc catttcatta gcaggattga aggttttaaa
     2341 tcctaaatca aatcgaacta caaaaaaatt caagtcatat cggagtccaa atcctgttcc
     2401 gagtgctagt tcttttagat cttgaattcc atcgaacgtg gctcttttat cagtaatatt
     2461 gtcaagtacg ttccaaatgt tgccagcatc ggcaaaaatg gctcctttga cacttcctaa
     2521 tattttaaat ctaaattcgg cgctcaaagc gattttcata ttggcctcat tgtagtcatt
     2581 agtagcgcct gtacttcctg gccctaaacg ataggattcc caagctctgt tatcattaga
     2641 tcctcctgca aaataacttc ttgaaaacgg aatgtttttt gaatttccgt acggaattgc
     2701 tatcccaaaa aagcttctaa cggccagaac tttttctttt gagaaatccc aatatttaat
     2761 ataatcgaat tctgttttta tatattctga atattctaag ttgaaaattt cataattgcc
     2821 atttgcgttt ttaggtaaat ttgctgcatt tgaaatcaat gacaaaaccg atccagccga
     2881 ttcaattttg gttctgaatt gatagaaagt attgtctttt agatcggatt ttgtggtttt
     2941 tgaaaacgaa taactggtag ataaaataaa gtcatcttca gttagtcgtg tttttcgttc
     3001 atcaatactg ctcacagttt cgtaatctgt atctgaaacg gtaattgctt tagttaaaac
     3061 ttgatctgta aatccatctg taccttcttc tattaataaa tttccatcaa tgtcaacatg
     3121 ttcagtataa ttgtatgttt ttgcaaggtt gtttaattca ttataggaag actcataaac
     3181 attataataa ttttcaggat ttaaatttcg tacatattgg acattgaaaa tatcgaatcg
     3241 agcggtatta tttttttttg ggttccaatt ataagcgagc attcctgtaa cattttgttt
     3301 gtctaaacca atgttttttt ggtttgataa acctacagac actaaagtcg agggaatcat
     3361 acttttagga ataatttttt cggtggcaaa aggaaaccaa attcgaggaa aatttaattt
     3421 agcgtcaatt ccatattcag acacattaaa aaacttgctt tgaggattgg ccaaatcttt
     3481 agaagaacca atgtttccgc gtgcagaaag ttctagtgtt tcggctccat taaatacatt
     3541 tcgaatggtt tcagaaatgc ttccagcaat tccaatgtct tgaatgttcg aatgcgtcac
     3601 atctaatgtt gtttgtaaac tgtatttttt tcgaggggat aaatacactt ttgcaataag
     3661 cgattgggct gtggtatctc ttttatctac ttcatataaa atggttggat aattaaaaat
     3721 tttcagattg ttaaggtatc gtgttgtcaa aacggttcta aaatcagcaa ataaacttcc
     3781 tttggtaata aaaacagcat cggtaattgc cttaggtttg tatttcaatt tagagtgact
     3841 gtacaaattg aaattattat aggtggtgct atcggcaaat acagcgctat tattggtagc
     3901 agaataatcg gtataaatgt ttacatcgct tattttgtac attttgaacg gttctgttct
     3961 tgttgtgtct ttgtcttgat aagaataatc tttaatgatc aaattgacat ttactttttt
     4021 gttgtggttc aacgtatcta aatcaaacgt cacataatta ggctgaaaat tatagactcc
     4081 attatttctg aggtgtgtac taatgcgttc tttttcgttg gtgaaatctt cggtattgta
     4141 ttgtttgcca gtctttataa aagagttgga aatttttgtt tgatacaatg aatccaaaac
     4201 cggagtttga atagaggtcg atacagtgtc aattaaaaaa ggatttccag tagtaatggt
     4261 gtattttatt tttgcttttt tctcacttaa actgtctaat ttgtaggcag cttttacatc
     4321 aaaaaaacct ttgttaaaat aataatattt tactcttgca atagattttt gagttcttgc
     4381 tgtgtcaaca attacaggtg gttcgccagt ttcttttaaa aaattatgaa aaccatgata
     4441 ccaaaaagat tgtcccaatc gatcgacttg tttggccgat aaccattttg acattcgttc
     4501 gtatttcttt ggattattag taaacttcgc ttgaaaagca gaatcaggat tgggtttggc
     4561 aagattaaac aaattcaatc gcagatgata ccccagaaaa gtggaatttg gtttttggta
     4621 caattgattg gtaatgtctt cgcttgttgt tgatttgtca tttacgacaa tttcattttt
     4681 agtgaggagc tgtttgcctt taggaacgcg cttctcagca tcacatgccg agattattat
     4741 tccaattaca ataaatgttg ctatttttgt gctaatattt ttcaagtgtt gggaaatatt
     4801 taattcaaaa gtacattatt ttatgcttag taaaaaccaa atcaagctaa ttacaagttt
     4861 acaacaaaaa aaacagcgaa tagcttctca attgtttttt gccgaaggga taaaagtaat
     4921 tcatgaattg ctagaatcaa agtttgaatt ggttcatttg tacaccactc aaaacgattt
     4981 tcaggaagtt tcgaatcata aaaaagtatt gattgacgat agtgaattga aaaaaattac
     5041 ggcattggct acagccaata cttgtttggc ggtatttaaa atcccatccg aaaaaaaaat
     5101 catcgactca ggattaattc ttgcgttgga ttcggttcgt gatcctggaa atttaggaac
     5161 cattttgcgt ttgtgtgact ggtttggtat tgaccaactc atttgttcaa gagaaacggt
     5221 cgatatttac aatccaaaag tagttcaggc tacaatgggg tctattgcaa gagtcaatgt
     5281 caattatatt gatttagaat cctttattgt tcaaacaaaa ttacctgttt tcggaacttt
     5341 tatggatggg aaaaatattt ataaagaaaa cctgcctcaa gaaggaatta tcattatggg
     5401 taatgaagcc aatgggattt cgcctacttt agaaaaatta atacaaaata gacttaccat
     5461 tccacgattt ggaacacttc aaaaaacaga aagtttgaat gtagctacag caacagcaat
     5521 tgttttgagc gagtttcgac gaggataata tttttataac attataaaca gctaactttt
     5581 tctagtgaaa agtaaaatta ataaaaacgg ctctactttt taaagactca atattgccag
     5641 tccaaggact atctggattt ttgtcacgaa ttagttcatc ggttagtcca aatacacctc
     5701 tgatggaagg tgaaaaaata aaatattccg aaaaaatatc aattccaaag cccaattcat
     5761 agtttgtagt ccaggatttt actctaaaaa tctgttcgta attgtcgtct atagattttg
     5821 cattgctaga caaatttaat gttgccgaaa gcccgcccaa tatataaggt ctaacatttc
     5881 ctgttctgag cgaagaaaat ttaagtaaca atggaaaatg aatgtaagta ctattaacct
     5941 ctcgcaacct atcagaatag gttggagttg attcaaaata actgcttggg tagcccaaat
     6001 ctcttttggt gtaatataaa ccgggttcaa aacgcaaatt aatatattct tgaagtcgca
     6061 aatcagcaac aagtcccaca ttgaaacctg tatttttttt cacttgaata tcttcactta
     6121 ctgttttgta atctactttg aaatcatagg aattaaaacc caaatagaat ccaaaataca
     6181 atttttgctt ttgaaaattt tctagattta taataggatc tttgccaaag agtttattct
     6241 gtgcaaaacc ttgtgtcgaa agggctaata aaattaaaac aactattttt ttcatattat
     6301 tttttggaag ctgaataaat ggtggccacg ccaaaagttt gaggcaaagc aactacatct
     6361 ataaacccaa tttttttcaa aatattgttc agagcctctc cataaggaaa agctgccgcc
     6421 gattctgata aatatccgta ggccacattg tcttttgaaa ataatttccc aatgattgga
     6481 agtatgtttt tactataaaa agtgtagcct tgtttgtaag gtgttttttc tggaaccgaa
     6541 gtttcgagga tgacgaatgt tccatttggt ttcaaaactc ttaaaatttc agtcaagcct
     6601 ttttctaaat tctcgaaatt tcgtacccca aaagcgactg ttatggcatc gaaataattg
     6661 tcttcaaatg gcatgttttc tgaatcggcc aaaaccatct caattcgatt agataaattt
     6721 ttgtcttgaa tttttgtctt tcccacttca agcattcctt gagaaatatc caaaccaatg
     6781 attttttcgg cctttgtttg tgccatcaaa atagctaaat ctcccgttcc tgttgcaata
     6841 tccaaaatgg tttttggatt gtttttggct acaatttgca aaactttttt gcgccatttc
     6901 acatcaattc caaaagaaat aacccgatta agattgtcat aattgcccga aatagtatca
     6961 aacatggtgg ctacttgctc ttttttgctt aaagtcgaat ctttataagg tgttatattt
     7021 tttgacattt tctaaaattt gtgacaaagg taaactagat ttttggataa taaatggtaa
     7081 actgaaattt tgggttgaaa taaggatttt tatgtttttc taacaaaggt ttgaaaagta
     7141 taatcataaa gatgtttttc atctttgtga ttaaaaatct ctgaagttaa ttcccaaatt
     7201 ttggaattaa tttctgggaa atagacatca gcttcaaacg aatgatgtac ccttgtaatg
     7261 tcaagttgat ctgctaaatg aatcgattgt gagtaaattt cgcctccgcc aataacaaat
     7321 gcatcttcgt tttttgggca aacagcaatg gcgctttcta aatcttgaac cacaatgcaa
     7381 ccttcgtgaa catagtcttt ttgccgagta ataatgacgt gagttctgtt tggtaaaggt
     7441 ttggggaaac tttcaaaagt ttttctaccc attatgatat gatgtcctga ggtaatttct
     7501 ttaaatcgtt taaaatcttt gggtaaatgc caaagtaagt cgttgttttt gccaagagca
     7561 ttgttttcgg caacagccgc aatcaaaata atcatgtagt gttgttctta tttggtagaa
     7621 ttctcttcta tttggttgtg caattgtacg attttttgtt gttgtttagc aacaagttta
     7681 tcaatttggt ctctttccca atttttattc ataaaacgat cggtaatata cacttttata
     7741 aaatgcaaaa taaagaggaa agcccaaaat gtaatcaccc aaatagccca atttgctgta
     7801 aacccaaata ttgaataatg attagcaata aacataaata tactgcctaa aaccaatagt
     7861 acaaaatgaa agtaaagtcc ctttttttgt tttagccttt ttctggcata ttcatattgt
     7921 tcgtgttgtt ctttttccat gaagcaagat tttgattata aagataaaat attttttgaa
     7981 atttcatcat tttttaattt aagaatattc aaagtgaaaa cgtttttgga tttagaatat
     8041 ctgaattttt attgataaaa attggtgatg ctatttatta ttaagagttt atttgtcgaa
     8101 atcctaaaac ttgtcttagg atgttgaatc ttcggcagaa tttgtttcct ttgcggcata
     8161 aatctataaa tgaaatagtt atgtcaatca agaaacaatt tataaaaaca aaaccagttt
     8221 gtaaagttac attttcggtt gaggcaaaag aagcgactac tgcatcggta gtaggtgact
     8281 tcaataattg gaattcggca gaaggggaat tatcaaaatt aaaaaatggt actttcaaag
     8341 gcgttttcga attaccaaaa gatacgacat atgaatttaa gtatcttgtt gatggagttt
     8401 atgttaatga gcctgaagca gattatttca tatggaatga atttgcagga gccgaaaata
     8461 gtgtattaga agtttaaaaa tattaaaaaa gtccgcaatt agcggacttt tttgttttta
     8521 agtaaatgga agcaggcttt ataatcccgc ttttattttc tcaatgactt cagcatattc
     8581 ggcatcagta agagtagttt tttgagggtt catttcgaaa acccctccaa agccataccc
     8641 atctttgtat tttggaacga tgtgcatatg caaatgtgat aaggtatcag cataagcgcc
     8701 atagtttatt ttggttggat tgaatgctgc tgctattgct tttgccgttg ttgcaacatc
     8761 gcgcataaaa gcattgcgtt ggtcgtcgct tagttcgtga aattccacgc cgtggtcttt
     8821 gtaaaccaca ttgcagcgac ctgtatatga ttgttcttta aataggaata attgtgatac
     8881 ttctaaatca caaattttaa tcattaaatt gtgtaatgtt tcattgtctt gacaatataa
     8941 acattctgaa actggggttg gcatgttagg ttttttattt aatttttttt atttgtaaag
     9001 agagaacctt ttttacaccg caacacttcc ttttatagcg tcgtggggct cataattttc
     9061 tagtgtgaaa tcctcataag taaattcgaa aatgtttttg attgctggat tcaaaatcat
     9121 ttttggtaat aattttggtt tgcgcgacaa ttgcaattcg agttggtcaa aatggttgtt
     9181 gtaaatgtga gcatcaccaa aagtgtgaat gaattcgcct acttctaaat cacaaacttg
     9241 agcaatcatc atagtaaaca aggcatatga tgctatattg aaaggcactc caagaaaaat
     9301 atcggcactt ctttgataca attgacaaga taattttcca tcggcaacat aaaactggaa
     9361 gaaggcgtga caaggaggta aagctgcttt attgttggca acattttttg caaaagatat
     9421 ggatgtgtct ggcaaaacac ttggattcca ggccgaaaca agcattcttc tgctgtttgg
     9481 gttggctttt agctcattta taagatctgc aatttggtct atttcttcgc tattccagtt
     9541 gcgccattga tgaccataaa tagggcctaa atctccattt tcatcagccc aagcatccca
     9601 aatttttact ccgttatctt ttaagtattt gatattggtg tctcctttca agaaccaaag
     9661 tagttcatga atgattgatt ttaagtgtag ttttttggtg gttactatcg ggaaaccttc
     9721 ggccaaatca aatcgcattt gataaccaaa aacgcttttg gttccagttc cagttcgatc
     9781 tcctttttga tttccgtttt ctaaaacgtg ttttactaaa tctaaatatt gtttcattct
     9841 tggtttaaaa gtttaatgtt taaagtttaa aagtttcgga atttgaaacc ttaacatttg
     9901 ttaatcaaca ttttttttgc tacctgctac ctgaaattat ctttttgaaa tttcgtctct
     9961 aattttggct gctttttcgt aatcttcatg ttctacagcc tgttcgagta aatcatttag
    10021 ttgttctaaa gtatattttg aatacgttcc gcctgattca ttgctttctt ctttttgttt
    10081 gaaagtttct ggatttgaaa gcacatcgtc aatttcttga gcatcttttt cgggattcga
    10141 gggattggat tttaagaaaa tacccgcttt atctaatatg tttttgtacg taaaaatagg
    10201 tgcattaaat cgcaatgcca atgcaattgc atccgaagtt ctggcatcta ttatttcttc
    10261 gattttatct ctttcgcaaa tgatgcttga gtaaaaaacg ccatctacta atttgtgtat
    10321 gatgatttgt ttaaccacaa tgtcaaaacg atcggcaaaa tttttgaata aatcatgagt
    10381 caaagggcga ggtggtttta tttctttttc caaggcaatt gcaatagatt gagcttcgaa
    10441 agcacctata acaattggta gttttctttc tccatcgatt tcatttaata tcaaggcata
    10501 agctccattt tgggtttgac tatatgatat tccttttatg gataatttga ctaagctcat
    10561 ttttttagag gaaaagcacg gtgtgcttgt ttatataaag ttatttttaa taaatcagga
    10621 tgcaatttta aacaaaaaaa gcagaacaaa aaagctacct aaagcaaaga tacgattatc
    10681 ttatttttag gcagctattt ttggtattag aattatattt tttttaatgt tgcgctttaa
    10741 atactttcaa tttttcgatt aattgtggta caacttcaaa agcatctcct ataactccat
    10801 agtcggcaac tttgaagaat ggtgcttcag gatctgtgtt tatgactact tttactttgg
    10861 atgaattgat tccagcaatg tgttgtatag ctcctgaaat tcccactgca atatataaat
    10921 ttgcagcaac gggttttcct gtttggccta cgtgttctga atgaggtctc cagtctaaat
    10981 cagaaacagg ttttgagcaa gctgttgcgg ctccaagtac ttgtgctaat tcttcaatca
    11041 ttccccaatg ttcagggcct tttaatcctc ggcctccaga gacaacaatg tcagcatcgg
    11101 caatagaaac ttttccagaa actttttcga ttttttcaac ttttattcca aaatcagcct
    11161 caccaatggt tggattaaaa tcttcaacag ataaatttga tgtattttca aaaatgccat
    11221 aggagttttt ggctaagcca attattttta cttcgctact aatttcggta atattgaaag
    11281 ctttgttcga aaaagcattt cttttcactt gaaagggaga agtgcttatt ggcaattcta
    11341 caacattaga ggctaaacca gcttcgagcg aaacagcaac aagtggagca agataaatac
    11401 tatcagttgt tgaagatagt attaccagtt tagtcccttc tttttgtgcg gcttgtttga
    11461 tgacatcggc ataggctttt gctgaaaaaa tcgataattt ttcgttggta attttcaaaa
    11521 ctttgtccac accatatttt gataattccg aaacatcttc agtatttatg gttatggctg
    11581 ttactgtagt acctattgat tcagctactt ttttggcata ggaagctaat tcgaaagcta
    11641 cttttctgaa ttttccttct gcggattctg catatattaa tattgacata aatgtaaaag
    11701 tttagagttt agagtttaga gtttagagtt tagtaactat aaactgagac tgtaaactga
    11761 ttgttaaatt actttcgctt cgttgtgtaa caaattaatt aattcgtcca aattatctgc
    11821 tgcaactaat tttacagccg atttaggagc aggtttttca aatttaactg cttttgtgtt
    11881 gatggaggca tcaataggtt caactaccgt taaggttttt gttcgagctg tcataattcc
    11941 tctcatattc ggaatgcgta aatctttttc ttcgactaat cctttttgac caccaattat
    12001 tataggtaaa gtggttgaaa tggtttctct tccgccatca atttcacgaa ttgcttttac
    12061 gttagatcca tcaactaata aatcggtgca tgaatttaaa aaattatacc ctaaaattcc
    12121 cgctatcata ccaggaacca ttccaccatt ataatctaat gattctttgc cggcaatgat
    12181 aatatcgtaa gagccatttt tggcaacttc tgcaatttgt ttggcaacaa agaaactatc
    12241 cgttggattg gcatttatac gaatggcgtc atttgctcca atggccaatg cttttcttaa
    12301 agtaggctct gtttctgcat taccaacgtt tacaacggta atggttgctc cttgttgttc
    12361 ttgaaaccaa atagcacgag tcaatccata ttcatcgttc ggattgatga caaattgaac
    12421 gccattagta tcaaattgag agtcgccgtt ggcgaaatta atttttgaag tggtgtctgg
    12481 aacgtgactt atgcaaacta atattttcat atgtgggtat ttaatattct tagattctcg
    12541 cacaaaaata aaatattaat tcggatatta tactatgcgc gcataatatt ttttaacaat
    12601 tatatcgatt tcgtaaatct tttttaattt aaaattattg gtaatgcttt tttaagattc
    12661 atttacatat tattttaaac aaaaattatg cttttaactg atttaaaata ttatttttgc
    12721 ggttcgaaat ttacagaact aaatgaaaac tatgagaacg atacaattta gagaagccat
    12781 ttgtgaggcg atgagcgaag aaatgcgtcg cgatgagtca atatatttaa tgggtgaaga
    12841 agttgcagaa tataatggtg cgtacaaagc atctaaaggt atgcttgctg aatttggtga
    12901 aaaaagagta atcgatacac ctatcgcaga gctaggtttt actggtattg cagtaggatc
    12961 ggcaatgaat ggttgcagac caattgttga atatatgact ttcaactttt gtttagtagg
    13021 tatagatcaa attataaata atgctgctaa aatgcgtcaa atgacaggag gacaatttaa
    13081 tgttcctatc gtttttcgtg gcccaacagc ttctgcagga caattgggag caacgcactc
    13141 acaggcttta gaaaattggt ttgccaatac tccagggcta aaagtagtag ttccatcgaa
    13201 tgtatatgat gcaaaaggac ttttgaaatc ggctattcgt gataatgatc ctgtaatttt
    13261 tatggaatct gaacaaatgt atggcgacaa aggtgaagtt cctgatggcg aatataccat
    13321 tccaattggt gttgctgata tcaaaagagc aggaacagat gtaactatcg tttctttcgg
    13381 aaaaatcatt aaagaagctt acatagctgc cgatgaatta gctaaagaag gaatttcttg
    13441 tgaaattatc gatttaagaa cggttcgtcc aatggataat gaagcgattt taacttctgt
    13501 aaaaaagaca aatagattag taattcttga agaagcttgg ccttttgcta gtatttcttc
    13561 tgaaattacc tatatcgttc aagaacaagc tttcgatttt cttgatgcac caattcaacg
    13621 tattactact gcagatacac cagctccgta ttcaccagtt ttgctaaaag agtggttgcc
    13681 aaatgctaat gacgttgtca aagcggttaa aaaagtaatg aataaataag ctgttaaact
    13741 tttctgaata ttagagaatt taggaaatct aatataagaa atcaactata tttgaaactt
    13801 catcggaatc caaattcgat gaagtttttt tttacgatta tgctgaaaaa tattatttta
    13861 tatctgtctt ttattgttgt ttttacgagc gctgtttttt cgcaaaccaa agtaagtggt
    13921 gtcgttttag accagttaaa caaacctatt ccttttgcaa atgtagcctt caagaattca
    13981 agtgaaggtg tagtatgtag tgaagacggt gttttttata tagaatcttc caaagattat
    14041 aaaaccatca ttattacatc ggtaggctat tcagacaagg aaattgattt ggaaaaacct
    14101 attaattata attttaaaat tcaattaaaa gaaattgaaa cgctaaatga agttgtaatt
    14161 ttcaggggaa aaacatcaaa aaagaacaat ccagctttag atattcttag aaaaatttgg
    14221 gaaagaaggc gcaaaaacgg tttgcatttg tttaatcaat atcaaatgga aaagtacgaa
    14281 aaaatagagt ttgatatgaa cactattgac agtacttata tgaaaaacaa gttgttcaaa
    14341 ggaatggaat ttattttcaa tcatgttgat acttctaaag ttactggaaa aacctatttg
    14401 cctattttta ttaatgaagc cttgactgat gtttacggag ataataaatt gaagaaaatt
    14461 aaagagaaaa ctaaagccaa caaaacttcg ggtttcaacg ggaatcaaca aattctggct
    14521 tttgtaaacg atttatatac agattttgat atttatgaca atcaccttac ttttttcgat
    14581 aaaagtttta caagtccatt atcgacaaca ggaatagatg tctataatta tgttttaaga
    14641 gacagtgcct acattgatga caaaaagtgt tataacattg ttttctatcc aagacgcaaa
    14701 aatgaattga cttttaaagg tgatttttgg gtgagcgaca ccacttttgc tatcaaaaaa
    14761 atcaatatgg ctgccacacg aagtgccaat ataaattggg taaaagatat ctatatcgag
    14821 caggaatttg aagttatgaa tgattccatt tttttgatga caaaagatta tatgatgacg
    14881 gattttgctt taagtaagaa agaaaaatcc aaaggagttt atggaaaacg aacttcatta
    14941 tatcgagatc ataaattcaa catcgaaaaa ccggagaaat tttacaagga agaggtgaat
    15001 tatttagata aagaggtcta taacaaatcc gatgactatt gggaaaaaaa ccgattcgaa
    15061 aatcttacca aagatgaaaa aggggtttat aaaatgcttg atactctgca aacagtcaag
    15121 aaatttcaac aattatatag tttggttgaa gtattagata gtggttatct tcattttgga
    15181 aatttagatt atggccctat ttattcagta attggacaaa attcagtcga aggattgcgt
    15241 ttgcgaacag gagtaagaac ttatactggt ccaaatgatc cttggcgatt gcaagcgtat
    15301 gttgcatatg gatttaaaga tgataaattt aaatatggag taactggtaa atggatggtt
    15361 gaacctaaaa aaagggttat tatttcggca ggaaatcgta gagatataga acaaattggg
    15421 gcatcgctta ctactacaaa tgacattgta gggcgcaatt atgcatcttc tggcttgctt
    15481 acttttggga gcaataataa attaacaaat gtcaatttga gtaacgttca gattgaaatg
    15541 gagcctatta aaaatttgac tttttcgact ggagtttcct atcgaacgtt gctatcagct
    15601 tcgccagaat tcagtttgga ttattatact gatttatcac aaaccaaaat tcaaagtcaa
    15661 gtaaaacaat ctgaagtaaa tttgcaagtt gaatacacgc ctaatagagt tcctatagct
    15721 tttggagtcg aaagagacaa tgcagatagc ccttttacta cgctgctggt gacttatagt
    15781 caaggattca agaacttatt ttcgagtgat ttcaactata aaaaactgca attgtattac
    15841 agacaaccta ttattattgg gccattagga cgatctaata taacttttga aacgggaaaa
    15901 acatttggtt acattccact cggattgatg agcgttgtgc ctgggaatca atcgcttttt
    15961 gatatttcta atacttttag caatttgaaa tactacgaat ttgttacgga tcaatatgta
    16021 acttttaaat gggatcacga ttttcaaggg cgattttttg ctagaattcc ttttatgaga
    16081 aaattaaatt ggcgtgaaaa tattggtgta aaatcggttt acggaacggt ttcaaacgac
    16141 aacagaacca taaatgcttc aggtttggag taccatgcgc cagacaaagt ctattgggaa
    16201 tacagcgcag gcattggcaa tattttcaaa ttttttcgtg ttgatttttc atggagaggc
    16261 aattatttaa atgtgcccga tgccaataaa tttgctacaa aaatatcatt tggattctat
    16321 ttctaatatg gaaaatgcga ttcaatatct tttagaaaaa gaagctattt ttaagattat
    16381 tatcgaaaaa tatggcttgc cgacaattcc aaaacgacca caaggatttg aaactttggt
    16441 tttattaatt cttgaacaac aagtttctat agattcggcc aaagccactt ttttaaaatt
    16501 aaaagctgaa caacccaatt tcaatcctaa atcgctattt tctttttcgg atgaggcgtt
    16561 tcggaacatt ggcgttagcc gacaaaaaac gtcctacata aaggctttat ctcattcgat
    16621 tattaacaat tatattgatt tagaaagttt tgcaaccaaa tcatcagaac aagttcgtga
    16681 ggaattaatc aaaatcaaag gcataggcca ttggaccatc gatatttatt tgatgttttc
    16741 actccaagct ccagatatta ttcctcttgg agatattgcc gtagtaaata ccatcaagga
    16801 gttgctggac attcacgaca agaaggaaat ggaagtttat gttgcaaaat ggagtcctta
    16861 tcaatctatt gccacttttc ttttgtggca ttattatttg aataaaagaa accggaaact
    16921 gttgtattga aaagttattt aaacgcagaa ataggctatt tatcgatttt atgttgtata
    16981 agttcttagt ttatctattt ttgtgctact aaataacaaa aaacaaaaat ggcagcagat
    17041 aaaattacca ctttcgatgt cttgattgag atacctagag gaagcagaaa taaatatgaa
    17101 tatgatttca aattgaaacg aattcgattt gacagaatgt tattttcatc gatgatgtat
    17161 cccgccgatt atggttttat gcctcaaaca ttagcactag acggcgatcc acttgatgta
    17221 ttggtattaa taaacgagcc aacttttcct ggttgtgtaa tggaggtgaa accaattggg
    17281 gtttttcata tggccgatga taaagggcca gacgaaaaaa taatttgtgt tcctgtttca
    17341 gatccgattt ggaattcttt gagtgatttg tcagacatca atccccattt attgaaagaa
    17401 atcgaacatt tctttcaggt ttacaaagac cttgaaaata aggttgtcga tgtaggaggt
    17461 tggggagatc ttagtgaagc tctagcgatc atcgatgaat gcacagagcg tttcaatcag
    17521 atcgagaaca aaccagcaga tttatttaat atttaataaa tacacttcaa tttttttcat
    17581 acaaagcaat attcattcgt ttgaatattg cttttttagt ttcaatatag tacgtttgca
    17641 tcataactgt gtaattttaa cctaaaacac aacaaaatta actttatgaa tgcaataatg
    17701 atttacgtgc ctatgctaat ggcattactt ggattggttt ttatgttttt aaaaagagct
    17761 tgggtattaa aacaagattc tggggatggt aaaatgaaag aaatttcaga atacatccac
    17821 gaaggagctt tagcttttct aaaagcagaa taccgattga tgactctgtt tgtaattgtg
    17881 gccagtattg ttttggctgg catcactttt ttgcctaatg caacaacgca tttattaata
    17941 gtggttgctt ttgtttttgg tgcctttttc tcagctttgg ccggaaatat gggaatgaaa
    18001 atagcaacaa aaactaatgt tagaacgact caagccgcaa gaacaagttt gccacaggcc
    18061 ttgaaagttt cttttggtgg tggaactgta atggggttag gtgttgcagg attggcagtt
    18121 ttaggactta caggattttt catcatcctt tttaatgtat atatgaatgg cgtttggacg
    18181 tcaactgaag atatgactgt tgtgctagaa acattagctg gtttctctct tggagcagaa
    18241 tctattgctt tgttcgctag agttggaggc ggaatttata caaaagctgc cgatgttggt
    18301 gcagatatcg ttggtaaggt cgaagctgga atcccagaag acgatcctag aaatcctgct
    18361 acaattgctg ataatgttgg agacaacgta ggggatgtag ccggaatggg agccgatttg
    18421 tttggttctt atgtagcaac agttcttgca gcaatggttc ttggtaatta tgtaataaaa
    18481 gatatgggtg gaaacattca agatgctttt ggtggaattg gtccaatatt attgccaatg
    18541 gcaattgctg gtttcggaat tttattttcc gtagtaggaa ctatgttggt taaaattaat
    18601 tctaatgatg ctaaagaagc tgatgtacaa aaagctttga atattggaaa ctgggcttct
    18661 attttgctaa caggacttgc ctgttatttc ttagttgatt atatgttgcc ttctaaaatg
    18721 aatatgagtt tctttggtga aggtttaaaa gaaatagcat caatacgagt attttatgct
    18781 tcaatggtag gattagttgt aggcgcaatc atttcttctg ttactgaata ctatacagga
    18841 ttaggtacaa aaccagtttt ggcaattgtt gcaaaatctt caacaggagc aggaaccaat
    18901 attattgcag gattatcaac aggaatgatg tccacttttc caaaagtaat tgtactagct
    18961 gcagctatct ggatttcgta ttcttttgct ggtttctacg gagtggcgct tgcggcttct
    19021 gcaatgatgg caacaacggc tatgcaatta gcaatcgatg cttttggacc tatatcggac
    19081 aacgcaggtg gaattgctga aatgagtgaa ttgccaaaag aagtacgcac cagaacagat
    19141 attttagatt cagttggaaa caccacagct gcaactggaa aaggatttgc catcgcttcg
    19201 gcagcaatga cttctttagg attgtttgct gcttatgtta cttttacagg aatcgacgga
    19261 atcaatattt ttaaagcccc tgttttagct atgttattta ttggcgcaat gattccagta
    19321 gttttctccg ctttagtaat gaattcagtt ggaaaagcag cgatggatat ggtgaatgag
    19381 gtgcgtcgtc agtttaaaga tattccagga attatggaag gtaccggaaa gccagaatat
    19441 gcaaaatgtg tcgatatttc tacaaaagcc gctttgaacg aaatgctgct tcctggtatt
    19501 ttgaccattg gttttccaat tggaattgta ttattgggta aactagttta tatgaacgat
    19561 gccaatgcca attcaatggt agccgaaatg cttgggggtt atatggccgg ggtaacagtt
    19621 tctggtgtag tttgggctat ttttcaaaac aatgctgaag gagcttggga caatgctaaa
    19681 aaatcttttg aagctggagt attagttgat ggcgcaatga gtttcaaagg ttcggatgca
    19741 cacaaagcgg ccgtaactgg agatactgtt ggagatcctt tcaaagatac ttctgggcca
    19801 tcgatgaata ttttaattaa attgacttgt ttgattggat tagtaattgc tccaattcta
    19861 ggaaatggca gtgctagttc agatgacaag atgattaaat gcaaaatgga aatgaagatg
    19921 tcttgtccaa tgggaaaaga agcaatgcca atgggttgcg atatgtctaa atgtgccacc
    19981 atgaccaaag atgaatgtgc aaaaatgtgt gacgaaaaag gatgttcacc agagcaaaaa
    20041 gaaatgtgtt tgtctcatta tgatgctaat ggtaagtttg ttgcagataa aaaagaaaat
    20101 tgttgtgcca aaaaaacaga ctgttttgat actagtaaat acgagaagaa aaacgttaaa
    20161 gttgaaatga ctaacaacga tggaaaagca aaagcaatcg ttaccacttc tgaaaatgga
    20221 aatgtaaacg ttcaagtttt cgaaggttct cttgaagatg ttaaagcaaa agtagaagct
    20281 ttgaaataat ttttttttct taattatata aaaatgcctc acagattttg aatatgtgag
    20341 gcattttatg ttttataaat tggggttaat tattgttcta aagcatattc ttttattaaa
    20401 gtagaagcgg gaatatgaag gttttcatta agcaaacgaa tttgtctaat tgttaaagct
    20461 cttttgcgtt tgaaaatttc agttactcga gaacgactat ttaaaataac gcccaaatcc
    20521 gcatttgaaa gtccgttttg ttccatcata aatttaatag cttcgattgg atctggttcc
    20581 ggaatagggt agtgaatttg ttcgtatttt tctatgagcg taactaaaat atctaattca
    20641 tctccttcat tggtattggg tttcgcatca aaaagggtgt ttactctttc taaagcaaag
    20701 ttgtaatctt tttctgtttt tataggacgt atttccattt ttataagtca tcagaaatat
    20761 attttaaaag ttcatcaagt tctttttgag aaagtgtttc ttgatcgctt ttgtcataaa
    20821 tagagagtaa atagacggta tctttagaga tgacaatatg agttatgatt ctcgctcctc
    20881 cagattttcc ttttcctttg gaagctatcg aaattctaat tttataacag tttttaccaa
    20941 ttctatttcc ttgttcggga ttttctttta gttcctgaac caattgtatt agttcgcttt
    21001 ttagggaagg atatttcttc aaaagccttt tcgtttgctt ttcaaaaaca gcaatcgttt
    21061 ttatattaaa ggtcattcaa aaagtcttca gcgttgcgag cttgtttttt gctagcttta
    21121 atcaaattta attcttgaac agcttctttt atttcttcta tcaaaacgaa ttttttacca
    21181 gagataggac tcgctttttt tacaaacgca agattacgca atacctctaa tccaaagtct
    21241 gctttattgt cgggaatatc tactaatact ttcataatct tgtgtgtttt ataaaacaaa
    21301 tttagtgaat aatttttaag aatatttcat ccctttaata tttcaaaaaa ttgtgaggag
    21361 ttttgctaaa ataaactttg ttgaaattat tattttagtt tatttgttgt taatcgtttt
    21421 gttgttaggg gtgattagct ttaaatttgg aaaaatatta ttaaacaaaa caaattatga
    21481 aaaatattat taaaccaagt gaaaatgaga caattaatat ttcagttatg gtaattcctt
    21541 ttgctctgca aattctttta ttttcgtgta ttatttcttt taattatgga aaagcaaaga
    21601 gttaactgtt tattttaaag gctgtctttt tcaagacagc ctttttagtt tttgttctaa
    21661 aacaacccat tcaattcagc atcgatttta ttgataatca ttcctaaatc ttctggatta
    21721 tctacaaaat taatgttgtc cacatcaata atcaataatt ttcctttggt gtaggtttga
    21781 atccaagctt catagcgttc gttcaatcgg ttgaggtaat ctatggaaat ggtgttttcg
    21841 tactcgcgac cgcgtttgtg aatttggcta actaaattag gaattgaact tctcaaataa
    21901 atcaacatat ctggagcttc taccatagac tccattaatt caaacaacga agaatagttt
    21961 tgaaaatcac gattggtcat caatcccata gcgtgaagat tgggcgcaaa aatatgggca
    22021 tcttcataaa tggttctatc ttgaatgatt tttttgccac tttcacgaat ttgcaacacc
    22081 tgacgaaaac ggctgttcaa gaaataaatt tgtaaattaa atgaccaacg ctccatttgg
    22141 tgataaaaat catccagata aggattgtca acaacgtctt caaaatgagg ttcccattta
    22201 aaatgtttgg ctaataaacg ggtcaatgtc gtttttccag ctcctatgtt tcctgctact
    22261 gctatgtgca ttatggtatt gtgattttat aatttgtaat ttcttggttg gtaaaaatag
    22321 acaaaatttg gtctttgtag aagaaatttt taaatgattt ttccacattt tctatcgtgt
    22381 aaatactatt cgtttttaaa tcttgtagaa acaattttcc atctttcaaa aaaacaattt
    22441 cttggttaga aatgatttga atctgatcaa agtcagcaat tttccccaaa gtggtaattt
    22501 ttccaaaaac atcacacgaa taccaattca gttggtcgtc aatccattga aaagtagtaa
    22561 agctggtttc gtaatatttt aaatttccgc tcaaagatgg tgttaaagtt tgaattgtgt
    22621 tttttaaata atcaaaaaaa cccaattttt gggtcaaact attgtaaacc caaagccgat
    22681 tttgagaagc aattcccgca gcattagcca ctattggaat atcattttcc gaaaaattaa
    22741 ttttttgagt ttcactcaat tgattgtcca aaaggattgc ggtgttgaag ttttcataaa
    22801 acaacataat ctttaatgga ttttgtatgt cgatttttgt gggttttcca agagaaatgt
    22861 ttttgtattc gaatgattct tttgcattat ttttagaaag aacattgttt tttatcgaat
    22921 aataatatcc aaatgaatcg tatcctataa aataatcagt gtttagtgtt gttgagcttt
    22981 gtttgattgc aatacatttt tcatcttgtc caaacgaaaa agtgaagact gaaataaaag
    23041 ctataagtag aatgtttttc ataagtgtag caaattacaa aaaataggat tgcaagagtc
    23101 agatattttt aatttcaaaa agagattttt atcctcatca tcaaaaaaat acaattcgtc
    23161 tattggtttt cgcaactcgt atatttaatt tcaatttaaa gccatatagt tttacttttg
    23221 aatggattgt gttcaaaaaa gtagtttagg aattatttat aaatgaatta aaccttttta
    23281 gcagactatt tatctaaaca gctaaatagg tattaaaatt gtaacaaaaa aaacttttaa
    23341 tagtcaaatt gagaatataa aaaaattaga attatgttaa aaattattca ttatgtcaca
    23401 ttatttttgg ctatcaattt cggacttcaa gcccaggaat ttcaaggaat ggctatttat
    23461 cagtctaaaa caagtactga agatttcaaa gctcgaatgg ctggaaacaa agacattact
    23521 cccgaaatgc aaaaaaatat ggaagaaaga atgaaaaaaa tgttcgaaaa aacattcatt
    23581 ctcaatttcg ataaaacagc ttctatttat aaagaagaag aaaaactaga agcaccaaca
    23641 caaggtggag gtggttttag aatgatgggt tcaatgatgg gagctggagg aacattttac
    23701 aaaaacatta aagaaaaatc atatactgtc gataaagagt ttatgggaaa agaattttta
    23761 attaaagatt ctttacctcg tttaaaatgg aaaatggaag gtgaaacccg agtaattggc
    23821 ggattcaatt gtttcaaagc aacagccgtt cgtccagtaa gtaaaaccga ttttcaaaat
    23881 ttaagaccta aaaaagagga tgagtcagcg gctaaaccaa agacagagga taagaaaact
    23941 gattttatga gtggattaga aatgccaaaa gaagtggttg taacggcttg gtacactcct
    24001 gaaattccag taaatcaagg tcctgacagt tattgggggc ttcctggatt aattttagaa
    24061 atcaatgatg gtaaaactgt tattttatgt tctaaagtgg ttttaaatcc aaaagaaaaa
    24121 gcagaaataa aagcctcaac caaagggaag ctaatttcgc aagcggaata tgatgaaacc
    24181 gtgatcaaaa aaatggaaga attccgtgaa atgcgttctc gtggaggaaa cagtggaccg
    24241 ccgcgaatga attaaaacag tattattaaa aactcgaagt attttatagt tcaaatacca
    24301 cctaatgaaa aatatatttt ttgctgttct tttttgtgtt agctcttttt cttttgccca
    24361 agctgtaaaa cttgaaggaa cgatttcaga cactaaatca cttcctgtag aaatggctaa
    24421 tgttatggcg gtaaaccaag ccacaaaagg aatggattct tatgctatta caaatgataa
    24481 aggaaaatac tccctgagtt tgaaacctaa cacaacttat agcatcaaaa ttagttttct
    24541 aggaatgcaa agcaaagagg ttgaaattgt aacaacagca acaaatatta gcaaaaatat
    24601 taccctagaa gacagtgctg tcgaacttga tggagtcgaa attattcgcg aaatgccggt
    24661 ttctatcaaa ggcgatacta ttgtttataa tgccgattct ttcaaatccg ggactgaaag
    24721 aaaactagaa gatgtgctaa aaaaattgcc aggagtcgaa gttaatgctg atggagaagt
    24781 agaagtagaa ggcaaaaaag tgaccaaatt aatggtcgaa ggcaaagatt tttttgatgg
    24841 cgataccaaa ttaggtgtca aaaatattcc tgccgatgct attgataaaa ttcaagtgct
    24901 tcgaaattat aacgaaaatt ccattcttaa aagcgtcgaa aacaatcaag acaatgtggc
    24961 gatgaatatc aaactcaaat ccggaaaaaa gaatttctgg tttggcgatt tgactgcagg
    25021 aatcggagtc ggaatgttgg atagtcgcta tttaattaac ccaaaattat tttattacag
    25081 tccaaaatac agtatcaatt taatcacaaa tttcaataat attggtgaat tgccacttac
    25141 cattcaggat tatatgaaat tgacaggtgg ttttcgagga ttatcagcta aaggtggttc
    25201 tagtttcaat attacttcca atgatttagg gatttcattg atgcgaaata acagagccaa
    25261 agaaattgaa actcaatttg gagcagcaaa tttctcctat agtgttaaca aagcttggac
    25321 tttgagcggt ttcggaattc tttcttcttc ggtcactgaa acggaaaccc tgtcgcaaac
    25381 gaattattta gatcccaatt cttctgagat tcaatcagtc gaaaacaaaa aagaattgtc
    25441 caatcaaaaa agcaacttgg gaatgtttaa actcagttcg agttacaaac cttcaactaa
    25501 ttttcagttt gattacgatg ttttagcgaa gctttcaaaa caacaagaag acgcaccttt
    25561 gtttcgagaa gcgattgtca attctatgac tacatcagaa gtgatcaata caaaaaaaga
    25621 gcaaaaaccc atttcggtca atcaaaacct gagtttgttc tatacgatga atgaaaaaaa
    25681 tatttttgca gtcgaaatgc agcatttgta tcaagaagaa gatccttttt ataatgcaaa
    25741 tttaaagacg caacctttta atttggtagg atatgtttct ggacaagata gaaataatat
    25801 cagtcaaaat cgttttgtaa cgaccagtaa gttagatgcg aaagtggatt attattatat
    25861 gattacgcca aaaagcaatt tcaatattac cttagggaat acctattcgt atcaaaattt
    25921 taattccagt atgtatcaaa ttctagacaa tcaggatgtc aatgaattag acagtcaggt
    25981 caatgtaaat gatgtgaatt atagatttaa cgatgccttt ttgggattgc attacaagat
    26041 tttaacgggt aaatttactt ttacaccagg ggtaagtttg cacagttaca atatgacgaa
    26101 cacgcaattg aacaccgatt acagtcaaaa cttcaccaga gctttacctg atttattagc
    26161 gatttatcaa atcaagaaag cggaatcttt gacttataat ttttcataca ctaataattt
    26221 taccgacatc aatcagttgt ctgaaggatt tgtgttctcc aattataata gtttacgaaa
    26281 aggaagtcga acactcgaaa acgcaactgc acgtcaacat tcattgcgtt atttcaaata
    26341 caatatgttt aatttagaaa acatttcggc ctttttgaat tattctaaaa gaattgatgc
    26401 cataaaaacc aaagcctttt ttgatggagt aaatcaatca tcggtgccct ataattcaga
    26461 ctttgccgac gaaacgcttt caggaagtgg ttcttatggg cgttctttct tgaagaatta
    26521 taaagcctct gtatcagcaa atatcaattg gtcgctattt aataatattc aaaataatgt
    26581 gtcatccaaa aaagagagtt ttactcaaag ctataccctg agaacttcaa ccaattataa
    26641 aaaattaccc aatttagaag taggttataa tattgtaatc aataattatg atagtaatgt
    26701 tttttatact gacaaaccct ttgctaaatt ggattattac tttttaaatg ccttttcgtt
    26761 cgtggcagaa tatgaatttt atcattatta caacaccgac aaaacggtca ataatcaata
    26821 tgattttctg aatgcaagtc taatttatca aaagaaagac agcaaattcg aatataaaat
    26881 tggcgcaaca aatctcttaa atacgaagtc tatcaacgat gacagtttct cacaattttc
    26941 tactagaact tcacaatata ctgttcaacc tcgctattta attttctctt taaagtataa
    27001 tatttaggag cttaatccag ctatccatta caatcttcgc tgaaaaagat agtttttc
//
LOCUS       DAOSNN010000008        24804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_2020_length_24804_cov_5.450119, whole genome shotgun sequence.
ACCESSION   DAOSNN010000008 DAOSNN010000000
VERSION     DAOSNN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 24804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24804
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2020_length_24804_cov_5.450119"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLQ70_00850"
     CDS             complement(<1..710)
                     /locus_tag="PLQ70_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HQE33193.1"
                     /translation="MKINFQKTYKFAVISALYISLFASFFALFLAKAIFKIPFKSLLL
                     FGFFFLLFVYIFSFIVLQYRVEKFIYRRVKKIYDDISLLESSTFIDQPITTDMEALTR
                     EVKKFATDKKLEIEMLKVREKYRREFLGNVSHELKTPLFTVQGYISTLLDGAMEDKNI
                     RKKYLKRAEKGVERLIYIVEDLDMITKLEVGDLNLEISEFDIVELIQNVFDLLEMKAD
                     KKKITLAFENYHIQPVFV"
     gene            complement(749..1432)
                     /locus_tag="PLQ70_00855"
     CDS             complement(749..1432)
                     /locus_tag="PLQ70_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQE33194.1"
                     /translation="MKKRNTKILLVDDEPDILEIVGYNLSQEGYQIYTASNGKEAVAK
                     AKKEIPDLIIMDVMMPEMDGMEACEMIRKIPELNNVIITFLTARSEDYSQVAGFDAGA
                     DDYISKPIKPKVLVSKVKALLRRLKEQEKNSETLNVGGIEINREEYQIIKEGVVIALP
                     RKEFELFYLLASKPGKVFKRDEILDKVWGNEVVVGGRTIDVHIRKLREKIGDDYFKTI
                     KGVGYKFEV"
     gene            1856..2194
                     /locus_tag="PLQ70_00860"
     CDS             1856..2194
                     /locus_tag="PLQ70_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33195.1"
                     /translation="MAKNYFYITLLLGFFFTLSVSAQDGKQQSKLQETTVIEGLNLYP
                     NPVSNGKVYITSKNDLDKDITIFDVLGKKVLQTTLSSKELNISNLTPGVYIIKINEAE
                     STATRKLIVR"
     gene            2299..3282
                     /locus_tag="PLQ70_00865"
     CDS             2299..3282
                     /locus_tag="PLQ70_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33196.1"
                     /translation="MKKFYALSFILLSTLSFGQVTDTFTGTGLLSANGWTLHSGTASQ
                     LTISSGSLTYSGLIPTGNKIALVAGNSEDVNKSVGTAITTAAYYSAVLNLPNTTGLTT
                     TGDYSISLGAQVGATVTGLYGRLMLKTGVAANTFNIGLVNTSGTGTTPTYLTTDYPTG
                     TPVFVVVKYDRSNNTAYLFVNPALDSTEPIPGLTNATGTTAAPASIASICLRQAGNAT
                     IGTGNVEYDDVRAGSTWAYVTTSTLVLSAKQNEIARLNIYPNPVTDGTLYITSNSSEA
                     KTVAVYDILGKQVLNTKTSNNAVNVSNLKGGAYIVKITEEGKTDTRKLIIE"
     gene            3477..4406
                     /locus_tag="PLQ70_00870"
     CDS             3477..4406
                     /locus_tag="PLQ70_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33197.1"
                     /translation="MKKLYTLSFVLLASLSFGQTPIGLIGVNVPYIEDFSGMGASLTT
                     YLPGWTAINVSNGATLSMAVTNGAASTGNAYNVGAIGDEERAFGTLADATITPALGAV
                     FQNNTGSTVSKISIQTRMEQWKESGNSGINETVAFYYSLDATSLSTGTWTPVSSLNLN
                     EKLTLATTNSAVNGNLAANYTNMANIISGLNWANGANLWIKWVDTNDSGTNGMYAIDN
                     FIISVNQVLGVKQNTIAGLNMYPNPVSKGTLYITSNSSEAKSVSVFDLLGKQVLNTKT
                     SNSTVNVSSLRAGSYIVKITEEGKTDTKKLIIE"
     gene            4488..5468
                     /locus_tag="PLQ70_00875"
     CDS             4488..5468
                     /locus_tag="PLQ70_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HQE33198.1"
                     /translation="MIPTPDIFTISNQKQFEKVALKVFRYQYENNLVYREFCDFLKTD
                     VRKVKSLEQIPFLPIQFFKSHTVVSNTNTAKAIFTSSGTTGMATSKHFVTDVFLYEES
                     YRKGFSQFYGNIEDYVVLALLPSYLEREGSSLIYMVEDLIQMTNNSESGFYLHNHDEL
                     IEKVIRLDNSGQNVILIGVTYALLDLIEKHSFQLQNTIIMETGGMKGKRKEMIREELH
                     QQLCEGFGVTAIHSEYGMTELLSQAYSLGNGVFECPSWMQILVRDTEDALTYVQQGKT
                     GGINVIDLANINSCSFIATQDLGKKNPNNSFEVLGRFDNSDIRGCNLMVI"
     gene            complement(5529..6821)
                     /gene="tyrS"
                     /locus_tag="PLQ70_00880"
     CDS             complement(5529..6821)
                     /gene="tyrS"
                     /locus_tag="PLQ70_00880"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013549833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQE33199.1"
                     /translation="MKNFIEEVTWRGMLHDVMPGTEEHLMEQMRVAYVGIDPTADSLH
                     IGHLVGVMLLKHFQISGHKPLALVGGATGMIGDPSGKSNERNLLDEATLRHNQEAIKE
                     QLSRFLDFTSNAANAAELVNNYDWMKNFSFLEFIRDVGKHITVNYMMAKDSVKKRLSS
                     EAAEGMSFTEFTYQLVQGYDFLHLYRDKSCTLQMGGSDQWGNITTGTELVRRIASGKA
                     YALTCPLITKADGTKFGKSEGGNIWLDAARTSPYKFYQYWLNTSDVDAEKYIKIFTFL
                     SKEEIEELTEKHKEAPHLRLLQKRLAEEITVMVHSKEDLENAIKASGILFGNSNAEDL
                     KQLDAATFLEVFDGVPQAEIAKSEIETGINIVTILNEKTGFFKSNGEARRALTANSIS
                     VNREKVTEEFVLSTKDLINNQFVLLQSGKKNYFVVRVV"
     gene            6935..7924
                     /locus_tag="PLQ70_00885"
     CDS             6935..7924
                     /locus_tag="PLQ70_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQE33200.1"
                     /translation="MILVTGGTGLVGAHLLLHLVENGESVRAIYRNLESIQKTKNLFS
                     IYQKGTLFEKINWIQADIIDIPSLEIAFKNVEYVYHCAALISFDPKDEDLLRKTNIEG
                     TANIVNFCLFNSIKKLCFVSSIAALGDLKENEKIITEETEWNPEKPHSDYAISKYGAE
                     MEIWRGQQEGLNSVIVNPGIIIGAGFWEQGSGLLFKKVEKGLLFFTKGSTGIIAVTDV
                     VKIMIQLMDSDVKNERFTLIAENITFQDLLNSIADSLKVKRPTIHATPFLVNIAYKID
                     WFLSNVLRQKRKLDRATAKASYTENHYSNAKIKTTLQTEFIAIHQYINDISKL"
     gene            complement(7925..8512)
                     /locus_tag="PLQ70_00890"
     CDS             complement(7925..8512)
                     /locus_tag="PLQ70_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HQE33201.1"
                     /translation="MKKILPFFIVLFILVGCKKELVKEPKRLIEREEMVNIMYDLSLL
                     EAMKVDNPILFDSLKNSQNQYIFKKYKIDSIQFVQSNIYYASDSKEYQKMFEKVKVRL
                     EKEKIQLASLQKAEAKKEILKAKKKKKLLEKKQSDSIKRVHIEVSKTKKKLSKKEVSD
                     SIKKAKTKAAKAKMTREIDSLKRIVAEQKRRQQIK"
     gene            complement(8522..9862)
                     /locus_tag="PLQ70_00895"
     CDS             complement(8522..9862)
                     /locus_tag="PLQ70_00895"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQE33202.1"
                     /translation="MNKVLIKNAKIVNEGTIFEGDVLIENDLIVEISDSISAKSSECK
                     IIDAEGNYLIPGAIDDQVHFREPGLTHKGDIESESRAAVAGGITSFIEQPNTVPNAVT
                     QEILEEKYQIAAEKSYANYSFMMGATNDNLEEVLKTNPKNVAGIKIFLGSSTGNMLVD
                     NEVVLEKIFSSTPMLIAVHCEDETTIKNNLEKFKAEYGEDIPVTAHHLIRSEEACFIS
                     SSKALALAKKTGARLHIFHLSTAKEMELFTNKIPLEDKKITAEVCVHHLWFSNEDYAT
                     KGNLIKWNPAVKTTNDRKVLWDALVDGRIDVIATDHAPHTLEEKKQSYLKAPSGGPLV
                     QHAVVAMFEAYHQGKISIEKIVEKMCHNPAKIFKIEKRGFIKEGYYADLVIVNSGLPW
                     SVKKENILYKCGWSPFEGFTFKSRITHTFVNGQMVYSASKVKDIRAGKRLSFDR"
     gene            complement(9875..10600)
                     /locus_tag="PLQ70_00900"
     CDS             complement(9875..10600)
                     /locus_tag="PLQ70_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HQE33203.1"
                     /translation="MNDCIVIIPTYNEIENIESILRAVLSQHKPFHVLVIDDNSPDHT
                     AEKVTLLQTEFEGRLFLEKRTGKLGLGTAYVHGFKWALEHNYSYIFEMDADFSHNPKD
                     LERLYDACHFGGADLAIGSRYVTGVNVVNWPLSRVLMSYFASVYVRFITGMKIHDATA
                     GFVCYKSEVLVGINLDKIKFVGYAFQIEMKYRTFCKKFDITEVPIIFTDRTKGQSKMS
                     SAIFKEAVLGVIALRLKKLFNTL"
     gene            10791..11462
                     /locus_tag="PLQ70_00905"
     CDS             10791..11462
                     /locus_tag="PLQ70_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HQE33204.1"
                     /translation="MIDNVLHLRVIENKDWATILFVLSFATVAITKSAFENRFAEFAK
                     LIYSDKYTNVYKEGSHLKSSFTIALFFVQLISFAFFIQLSFSYFGYSSKTDWVLFIQV
                     ITFLIYFVLSKFLIEKIIATAFNIEEIIEQFNLQKVIFRTYIGLFLLPVNIILFYNNA
                     ISRNFMIILISIVLIFNVIIYLVSIKNYQNIIFSKLFYFILYLCALEIAPYYFLYYWF
                     TKGIA"
     gene            11475..12224
                     /locus_tag="PLQ70_00910"
     CDS             11475..12224
                     /locus_tag="PLQ70_00910"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQE33205.1"
                     /translation="MKVKTILVSQPEPKVENSPYFELQNKHKVKVDFRPFIHVEGVSA
                     KEIRLQKIDLNHFTAIILTSKNAVDHFFRVADEMRYKVPEGLKYFCQSEAIAFYLQKY
                     VVYRKRKIYVGPKDFADLSPLIKKYKDEKFLLPASDQLNADAPITLNALKVDWTPAVF
                     YKTVISDLSDLADVYYDVLAFFSPTGIKSLFTNFPDFEQNNTRIAVFGSTTQKAALEK
                     GLRIDILAPTPETPSMTMALEKYISEANKGK"
     gene            12356..12700
                     /locus_tag="PLQ70_00915"
     CDS             12356..12700
                     /locus_tag="PLQ70_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33206.1"
                     /translation="MKKIPLIILMISLSLVIVNCSSSKKGTADPKISYKTDIAPIISN
                     SCTPCHMPPQGRKEPLENYIHVKENIVEILERVQLPQDNRKFMPPANKKPALTTDQIA
                     LLVKWQQQNMPE"
     gene            complement(12801..13481)
                     /locus_tag="PLQ70_00920"
     CDS             complement(12801..13481)
                     /locus_tag="PLQ70_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HQE33207.1"
                     /translation="MGMGYMLLAGLIMLASWLVSSRLKSKFEQYSKLHLQNGMSGAEI
                     AEKMLADNGIRDVRVISTPGQLTDHYNPVDKTVNLSEAVYNQRSAAAAAVAAHECGHA
                     VQHAVGYQWLTMRSQLVPIVNVASSYMQWILLAGILMIRTFPQLLLIGIVIFAATTLF
                     SIVTLPVEYDASNRALAWLENKRMLTQQEQAGAKDALKWAARTYVVAALGSIATLLYY
                     VSIYMNRR"
     gene            complement(13868..17368)
                     /locus_tag="PLQ70_00925"
     CDS             complement(13868..17368)
                     /locus_tag="PLQ70_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQE33208.1"
                     /translation="MKYNPNEIEAKWQKHWAENQTFSAENNSEKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDVYSRFKRHQGFNVLHPMGYDSFGLPAEQYAIQTGQRPEDTTSV
                     NIDGGVDKEGNKIAGYRKQLDKIGFSFDWSREVRTSNPDYYKHTQWIFIQLFNSWYNK
                     NTDKAEDISTLVSVFEKEGNANVNAVCDDNIVGFSSSEWNAFSSEEQQKILLQYRLTY
                     LAETEVNWCPGLGTVLANDEIVNGVSERGGFPVIRKKMTQWSMRISAYAERLLQGLDT
                     IDWSESIKESQRNWIGKSVGAMVSFAVKPHPQPLSKGEGSQAPGYMTANASIASQLVG
                     NARENRKNSTEAEDALWQEVRNSNLGYKIRRQHYIGNFIVDFVSLDKKLIIEVDGGYH
                     DTKEQIELDEQRTLELEQKHLYKVIRFTNEEVLNDIKSVLTKIKEELSLRNSYNINEE
                     LAPLSSGEGSGVRFIDVFTTRPDTIFGVTFMTLAPEHELVAQITTAEQKAEVEAYIEK
                     TAKRSERERMADVKTISGVFTGAYAEHPFTKEPIPVWIGDYVLAGYGTGAVMAVPCGD
                     ERDYAFANFFKGQNGMQDIKNIFDKDISESAFGAKEGFQLVDSDFLNGLGYKEGTKKV
                     ISELEKIGQGKGKINYRLRDAVFSRQRYWGEPFPVYYVNGLPQMIDAKHLPIVLPEVE
                     KYLPTEDGLPPLGNASVWAWDSVNNKVVDTNLVDNATIFPLELNTMPGWAGSSWYWMR
                     YMDAKNDGEFASKEALKYWESVDLYIGGSEHATGHLLYSRFWNKFLKDKGFAPTEEPF
                     KKLINQGMILGMSAFVKRIDLPNSYVISYRKGYENNDDSEIFKNLFISNGESKKIIEG
                     DSIILKKYFSDAIKKKYNVEDSKEVVEFIDIDKIEINNIHADVSFVNSSDELDIEKFK
                     SWREDYANAEFITEENGKYIVGREIEKMSKSKYNVVTPDDICNEYGADTLRLYEMFLG
                     PLEQAKPWNTAGISGVFGFLKKLWRLYFDENGSIVTNDTPTKDNLKSLHKTIKKVAED
                     IEGFSFNTSVSQFMICVNELSTQNCHSRAILEPLAILISPYAPHIAEELWAQLGNSGS
                     ISTVDFPVFEAKHLVESEKEYPVSFNGKMRFTITLPLDLTKEQIEEIVMKDERTQKQL
                     DGKTPNKVIIVPGKIINLVG"
     gene            17526..18401
                     /locus_tag="PLQ70_00930"
     CDS             17526..18401
                     /locus_tag="PLQ70_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HQE33209.1"
                     /translation="MASNFDKFQKRRLLSSYFSVVLSVFLVLFLLGILGFFIINSRKL
                     ANNFREEIAMTVFFKNDANDTILKAFAKELKVAKFTKSFVYVSKEKAAKEHTDIIGED
                     FMTFLGTNPLQNSYDIHLKADYVERDSIAKIESLLHKNPMVSDIVYDKQLVNLVNDNI
                     KKVSMWILIISIFLTFVAVLLINSSLRLSIYSHRFIIKTMQMVGATKAFIRKPFVMRS
                     IKLGMLGAGLAIIALIGVLIYIQTNFPGLGILDDKLLVALVLLLVFGVGVLITWISTY
                     FATQRFLNLRTDDLY"
     gene            18434..18676
                     /locus_tag="PLQ70_00935"
     CDS             18434..18676
                     /locus_tag="PLQ70_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015481682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HQE33210.1"
                     /translation="MKNKEEQKHEFLFEKTNYIILLIGIAVIALGFILMSGGGSDDPN
                     VFNEEIFNFRRIRLAPTTVLIGFGITIYAILKNPKK"
     gene            18744..19541
                     /locus_tag="PLQ70_00940"
     CDS             18744..19541
                     /locus_tag="PLQ70_00940"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQE33211.1"
                     /translation="MNTFQAIIIAIIEGITEFLPVSSTGHMILASSFFGIEHDDFTKL
                     FTIVIQLGAILSVVVLYFKRFFQSFDFYFKLLVAFIPAVVFGLLFSKKIDALLESPLT
                     VAVSLLIGGIILLKVDDWFTNAEEVQTSNEISYAQALKIGLFQCLAMVPGVSRSGASI
                     VGGMSQKLSRTSAAEFSFFLAVPTMLGATAKKCYDYYKDGLVLSHDQINFLIIGNLVA
                     FIVALIAIKSFIGFLTKNGFKVFGYYRIIAGILLLAIHFFIHPLTII"
     gene            19541..20235
                     /gene="truB"
                     /locus_tag="PLQ70_00945"
                     /pseudo
     CDS             19541..20235
                     /gene="truB"
                     /locus_tag="PLQ70_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
     gene            20681..21388
                     /gene="pyrH"
                     /locus_tag="PLQ70_00950"
     CDS             20681..21388
                     /gene="pyrH"
                     /locus_tag="PLQ70_00950"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQE33212.1"
                     /translation="MKYKRILLKLSGEALMGERQYGIDPARLGEYADEIKKIHTKGVE
                     IAIVIGGGNIFRGVAGASNGMDRVQGDYMGMLATVINGMALQGALEDKGMKTRLQTAL
                     KMESIAEPYIKRRADRHLEKGRIVIFGAGTGNPYFTTDTAAVLRGVEINADVILKGTR
                     VDGVYTADPEKDATATKFNYITFDDVLKKGLNVMDTTAFTLSQENKLPIVIFDMNKQG
                     NLLKICEGENVGTEVNI"
     gene            21459..22022
                     /gene="frr"
                     /locus_tag="PLQ70_00955"
     CDS             21459..22022
                     /gene="frr"
                     /locus_tag="PLQ70_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQE33213.1"
                     /translation="MTEEIEFILDSTQESMEGTIAHLEKSFLNIRAGKASPAMLGSVF
                     VDYYGSASPLSQVSKISVPDARTITLQPFEKNMLHPIEKAIMIANLGFNPMNNGDVII
                     ISVPPLTEDRRRELAKQAKAEAEDAKIGIRNARKDANTEIKKLEKDGTSEDICKKAEE
                     EVQNLTNSFIKKIDEHLVHKEAEIMKV"
     gene            complement(22075..24351)
                     /locus_tag="PLQ70_00960"
     CDS             complement(22075..24351)
                     /locus_tag="PLQ70_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQE33214.1"
                     /translation="MKNYRNTIFYLVVTGGFSALIYWILTMGKGLEVHKKYLRPVTEN
                     GHWNDFVDSISLNVHHPLAILLAQIVTIIIVARFFGWVFRKIGQPSVIGEIIAGIVLG
                     PSLLGLYFPEFSLALFPVDSLGNLQFLSQIGLILFMFVIGMELDLKVLQNRAKDAVVI
                     SHASIVIPFALGIGLAYFVYFRFAPEEVAFLPFALFMGIAMSITAFPVLARIVQERGI
                     NKTKLGAVVITCAAADDITAWCILAAVIAIVKAGTFVSSLYIIGMAMVYVLAMLFVVK
                     PFLAKIGELYATKDSINKPVVAIFFLTLIISSYTTEIIGIHALFGAFMTGVIMPDITK
                     FRNLFIEKVEDVSLILLLPLFFVFTGLRTEIGLINDPFLWRVTGFIILVAVIGKFLGS
                     ALAAKFVGQSWRDSFTIGALMNTRGLMELIVLNIGLDLKVLTPEVFTMMVIMALVTTF
                     MTGPTLNFINYIFKTKDDADLEEISIANIKKYKILISFGNNEKGKSLLRLANSLVKKQ
                     NEKAAITAMHLTLSDEVHAYNLEEYEKDKFLPIIEESKTLNQEITTLFQATVDIESNI
                     AEVANNGDYDLLLIGLGKSIFEGSLLGKVLGFTTRIINPDRLIDKFTGKEGLFENSPF
                     DERTRQIIAKTKTPLGILIDKDLQQVDRVIIPIFSSEDAFLLDYAQKLITNNNSKIKI
                     LDVNANSNDNFVLKSAINSLEQKYPKNIILIDENSIKKDLLEHLDLMIIGVESWKVLL
                     DKYSVWLRRVPSVLILKH"
     gene            24523..>24804
                     /locus_tag="PLQ70_00965"
     CDS             24523..>24804
                     /locus_tag="PLQ70_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND family transporter"
                     /protein_id="HQE33215.1"
                     /translation="MIKWFSLGFWELIARVVLRNRILMLSLVILTTVLLAMQWKNIHF
                     TQTEANMLPDDNIVNIEYNAFLNRFGEEGNLVIIGVKDKTFFTPKAYAAW"
BASE COUNT         8219 a         4533 c         3835 g         8217 t
ORIGIN      
        1 ttcacaaaaa cgggttgaat atgatagttt tcaaatgcca aagtaatttt ctttttatct
       61 gctttcatct ccaataaatc aaaaacattt tgaattaatt cgacaatatc aaattctgaa
      121 atttcgagat tcaaatcgcc tacttccaat ttggtaatca tatctaagtc ttcgacaata
      181 taaattaatc gttcgactcc cttttcggca cgcttcaaat atttttttcg aatattttta
      241 tcttccatcg caccatcaag tagtgtagaa atataacctt ggacggtaaa caacggggtt
      301 ttcaactcgt gagaaacatt gcccagaaat tctcttcggt acttttctcg aactttaagc
      361 atttctattt cgagtttttt gtcggtagca aactttttaa cttcccttgt caaggcttcc
      421 atatcagttg taataggctg atctataaat gtactggatt ctaaaagaga aatatcgtca
      481 tagatttttt ttacccttcg gtaaatgaat ttttcaactc tatattgcaa tacgataaag
      541 gaaaagatat agacaaagag aagaaaaaaa aatccaaaca aaagcaaact tttgaatgga
      601 attttaaata ttgcttttgc taaaaaaaga gcaaaaaaag aagcaaaaag acttatatac
      661 aaagccgata taacggcaaa cttataggtt ttttggaagt ttattttcat ttttttgagt
      721 tggttgttat tgcaaattta ctcaaaaatt acacttcaaa tttataccca acccctttta
      781 tggttttaaa gtaatcatca cctatttttt cacgaagttt tcgaatatga acatctattg
      841 ttcttccgcc aaccaccact tcgttgcccc aaactttatc tagtatttca tcacgtttga
      901 aaacttttcc tggttttgaa gctaataaat aaaacaattc aaactctttt cgaggcaaag
      961 caattacaac gccttctttt atgatttgat attcttcgcg attgatttcg attccgccaa
     1021 cattaagggt ttcactgttt ttttcctgct ctttcaacct tcttaacaat gccttaactt
     1081 tactaacaag cactttgggt tttatgggct tggatatgta atcatcagca cccgcatcaa
     1141 aacctgctac ttgggaataa tcttcgcttc gggcagtcaa aaaagtaatg attacattat
     1201 tcaattctgg aatttttcta atcatttcac aagcttccat tccatccatt tcaggcatca
     1261 ttacatccat aataataaga tctgggattt cttttttagc tttagcaaca gcctcttttc
     1321 cgttcgaagc ggtataaatt tgatagcctt cttgactgag attgtaacca acaatttcta
     1381 aaatatctgg ttcgtcatcg actaataaaa tcttggtgtt tcgttttttc ataatagcac
     1441 aatactgctt ttaggtttgt aaaggtaaat ataaaacaaa acaattgcat gtgtaaaacc
     1501 gttttttata agttaactat ttggtaatac atccgaaaca aattaataac actggaataa
     1561 caaatttata atgatttacc cctttaaacc tttcccacat tggatttact tatttagaaa
     1621 attcaactaa taatttctta aatacaagta atacaatttc taatgaagca aaaaaatgtc
     1681 tcagattttt ataaaaaatc taattagcac catcacaaat tgaaaagtaa agaatcaaat
     1741 tattatcaaa cttttctgaa tttcaatttt aaaaacccac tttaacgcaa ttttataaat
     1801 tttggtataa attttgaaat gtttttttat atctttacaa ctccaaatct atatgatggc
     1861 aaaaaattac ttttatatta ctcttttatt gggttttttc ttcactttga gcgtttcagc
     1921 tcaagatggt aagcaacaat ccaaacttca agaaaccaca gttattgaag ggttaaactt
     1981 gtaccctaat cctgtgagta atggaaaagt atatatcaca tcaaaaaatg accttgataa
     2041 agacatcact atttttgatg ttttaggaaa aaaagtactt caaactacat tgagttcaaa
     2101 agaacttaat atttctaatt taactcctgg agtttacatc atcaagatca acgaagctga
     2161 atctactgca acccgaaaac ttatcgttag gtaaaagaat ctcatatttt ttcacaattt
     2221 aatgtttttt ttacattgct ttattcttac aaagcgattt tattgttata tttgtataac
     2281 taatttaaaa caaatgttat gaaaaaattt tacgctttat cttttatttt attgagcact
     2341 ttgtcatttg gtcaagtaac agacactttt actggaacag gtttattaag cgcaaatggt
     2401 tggacacttc acagtggaac agcaagtcaa ttaaccatat catctggctc attaacttat
     2461 tcaggattaa taccaacagg aaataaaatc gcattagttg caggaaactc agaagacgtg
     2521 aataaaagtg ttggtaccgc aatcaccact gccgcatact attcagcggt tttaaacttg
     2581 ccaaacacta ctggcttaac aactactggg gattattcaa tctctcttgg cgcacaagta
     2641 ggagctactg ttactggatt gtatggaagg ttaatgctta aaacgggtgt tgccgcaaat
     2701 acttttaata ttggacttgt aaatactagc ggtacaggaa ctacaccaac ttatctgaca
     2761 accgattatc ctacaggcac acctgttttt gtagtagtta aatatgacag atcaaacaac
     2821 acagcttatt tatttgtaaa tccagcatta gatagtaccg aacctatacc aggtctaaca
     2881 aatgcaacag gaacaactgc tgctccagcc tcaattgcaa gtatttgctt aagacaagct
     2941 ggaaatgcaa caattggaac tggaaatgtt gaatatgatg atgtaagagc tggaagcact
     3001 tgggcttatg ttacaacttc tacgcttgtt ttatccgcaa aacaaaacga aatcgctaga
     3061 ttaaatatct atccaaatcc agtaactgat ggtactttat acatcacttc aaacagtagt
     3121 gaagcaaaaa ccgtagcggt ttatgacatt ttaggaaaac aagttttaaa cactaaaact
     3181 tcaaacaatg ctgtaaatgt ttccaactta aaaggcggtg cttacattgt aaaaattact
     3241 gaagaaggaa aaacagacac tagaaaatta attattgagt aattaattta ccatattctt
     3301 ttaaaagctc taatgaaaat tagggctttt ttttgtttta atcacacgat aaattacatc
     3361 taaaagtttc actggcaatt aattaaaaac cgataagtaa gaaaaaaagt agatttttct
     3421 ttcttacaaa gaaaatactt actatattta caaattaatt taaaattaca ccttttatga
     3481 aaaaacttta cacattatca tttgttttat tggcctcttt gtcctttgga caaacaccca
     3541 taggtcttat tggagtaaac gttccttaca ttgaggattt cagcggaatg ggtgcttcac
     3601 ttacaactta ccttcctggt tggactgcga ttaacgttag taacggagca acattatcca
     3661 tggcagtaac aaatggtgct gcaagcactg gaaacgcata caatgttggt gcaataggag
     3721 acgaagaaag agcatttgga acattggcag atgccacaat aactcctgcc ttaggtgccg
     3781 tttttcaaaa caatactggt agtacagttt caaaaatatc cattcaaact agaatggaac
     3841 aatggaaaga atcaggaaac tcgggtataa atgaaacagt tgctttttat tatagtttag
     3901 atgctacaag tttaagtaca ggaacttgga ctcctgtaag ctcattaaat ttaaacgaaa
     3961 aattaacatt ggccactact aactctgctg tcaatggaaa tttagcagct aattacacca
     4021 atatggcaaa tataatttct ggattgaact gggcaaatgg agccaattta tggattaaat
     4081 gggtagatac taacgactct ggaactaacg gaatgtatgc catcgataat ttcatcataa
     4141 gtgttaatca ggttttagga gttaaacaaa acaccattgc tggcttaaat atgtatccaa
     4201 atcctgtttc taaaggaact ttatatatca cttcaaacag tagcgaggca aaatcagttt
     4261 ctgtttttga tctattagga aaacaagttt tgaatacaaa aacttcaaac agcactgtta
     4321 atgtttctag cttgagagct ggaagttaca tagtgaaaat tacggaagaa ggaaaaacag
     4381 ataccaaaaa attaattata gaataattaa tttcaaacta tatttctaaa aactccaatt
     4441 gcaaagttgg agtttttttt atttaattac ttttgcaaaa aaaattcttg attccaactc
     4501 ccgacatatt caccatttca aaccaaaaac aatttgaaaa agtagcactc aaagtgtttc
     4561 gatatcaata tgaaaacaac ttggtttatc gggaattttg cgattttcta aaaaccgatg
     4621 tgcgaaaagt aaaatcgttg gaacaaattc cttttttacc gattcaattt ttcaaaagtc
     4681 atactgtagt ttctaatacc aataccgcca aagccatttt taccagcagc ggcacaaccg
     4741 gaatggcaac cagcaaacat ttcgttactg atgtttttct atatgaagaa agctatcgaa
     4801 aaggattttc tcagttttat ggtaatatcg aagattatgt agttctagct ttacttccat
     4861 cctatttgga acgtgaaggt tcgtcattaa tatatatggt cgaagatttg atccaaatga
     4921 ccaataattc tgaaagcgga ttttaccttc acaaccacga cgaactcatc gaaaaagtaa
     4981 ttcgattaga caattcagga caaaatgtaa ttttgatagg tgttacttat gctttattgg
     5041 atttaatcga aaaacattca ttccaattgc aaaacaccat tattatggaa acgggtggaa
     5101 tgaaaggcaa acgaaaggaa atgattcgag aagaattgca ccaacaactt tgcgaaggtt
     5161 ttggcgtcac cgccattcat tcagaatacg gtatgactga attattgtcg caagcttatt
     5221 ccctcgggaa tggtgtgttt gaatgccctt cttggatgca aatactagtt cgcgatactg
     5281 aagatgcgtt gacttatgtt caacaaggaa aaacaggcgg catcaacgtg attgatttgg
     5341 ccaacatcaa ttcctgctcc tttattgcca cgcaggattt gggtaaaaaa aatcccaaca
     5401 actctttcga ggtattggga cgttttgata attcagatat tcggggttgt aatttgatgg
     5461 tgatttaacc acaaagactc gaagaaggac aaagttcaca aagagaaact tagcatctca
     5521 cgttagattt aaaccactct taccacaaaa taatttttct taccactttg caacaaaacg
     5581 aattgattat tgattaaatc tttggttgaa agcacaaatt cttcggttac tttttctcga
     5641 tttacagaaa tagaattcgc agtcaaagct cttctcgctt caccattaga tttaaaaaat
     5701 cctgtttttt cgtttaaaat ggtaacaata ttgattccag tttcgatttc agatttcgca
     5761 atttccgctt gaggtacacc atcaaaaact tccaagaaag tcgcagcgtc caattgcttt
     5821 aaatcttcgg cattggaatt tccaaaaaga attccagacg cttttatggc attttctaaa
     5881 tcttctttag aatgaaccat aaccgtgatt tcttcagcca aacgtttttg taacaatctc
     5941 aaatgtggtg cttctttatg cttttcggtt aattcttcta tttcttcttt cgataaaaaa
     6001 gtaaaaatct tgatgtattt ctcagcatca acatctgaag tatttaacca atattggtaa
     6061 aatttataag gtgaagttct ggctgcatcc aaccaaatat tgcctccttc ggatttacca
     6121 aacttggttc catcggcttt tgtgattaac ggacaagtca atgcgtaagc ttttccgctg
     6181 gctattcttc gtactaattc ggttccagta gtaatatttc cccattggtc acttccgccc
     6241 atttgtagcg tacaactttt gtctctgtac aaatgtaaaa agtcgtatcc ttgaaccaat
     6301 tgatacgtaa actccgtaaa agacattcct tcggctgctt cagacgacaa acgtttcttt
     6361 acagaatctt tcgccatcat atagtttaca gtaatatgtt tacccacatc acgaatgaat
     6421 tccagaaaag aaaagttctt catccagtcg taattattca ctaattcggc agcattggca
     6481 gcattggaag taaaatctaa aaaacgggat aattgttctt ttatggcttc ttgattgtga
     6541 cgcaaagtag cttcgtccaa caaatttctt tcattcgatt ttccagatgg atccccaatc
     6601 attcctgtgg ctcctcctac taaagctaat ggtttatgtc cagaaatctg gaaatgtttc
     6661 aacaacatca ctcctactaa atgcccgata tgcaaggaat cggcagttgg gtcaatcccc
     6721 acataagcca ctcgcatttg ttccatcaaa tgttcttcgg tgcctggcat aacatcgtgg
     6781 agcattcctc tccaagtgac ttcttctata aaattcttca ttttttaaaa tcaattttgg
     6841 caaagataaa atataatgtt gattcggtta attgtttatt cggttaattg aaaaaaaatc
     6901 gcaatccgta atccgtaatt cgtaaattcg caaaatgatt ttagtcacag gaggaacagg
     6961 tttagtcggc gcacatttat tgcttcattt agttgaaaat ggagaaagtg ttcgtgccat
     7021 atatcgtaat ttggaatcca ttcaaaaaac caaaaatctt ttttctattt accaaaaagg
     7081 aactttattc gaaaaaataa actggattca ggctgatatt attgacattc cttcattgga
     7141 aatcgctttt aaaaacgtgg aatatgttta tcattgtgcc gctttaattt catttgaccc
     7201 aaaagacgaa gatttgcttc gaaaaaccaa tatagaagga acggccaaca tagttaattt
     7261 ttgtttgttc aacagcataa aaaaactttg ttttgtcagt tctattgctg ctctgggtga
     7321 tttaaaagaa aacgaaaaaa tcatcaccga agaaaccgaa tggaatcccg agaaacccca
     7381 tagtgattat gccatttcta aatatggagc cgaaatggaa atttggcgag ggcaacaaga
     7441 aggtttaaat tccgtaattg taaatcctgg aatcattatt ggagcaggtt tttgggaaca
     7501 aggcagcgga ttacttttta agaaagttga aaaaggattg ttatttttca ccaaaggctc
     7561 aacaggaatt attgccgtga ccgatgtggt aaaaattatg attcaattga tggatagcga
     7621 cgttaaaaac gaaagattta ctctaattgc agaaaatatt actttccaag acttgctgaa
     7681 ttctattgca gattctttga aagtaaaaag acccaccatt cacgcaactc catttttggt
     7741 aaatattgcc tataaaatag attggttctt gtcaaatgta ttaaggcaaa aaagaaaatt
     7801 agatcgtgct actgccaaag catcttatac tgaaaatcac tattcgaacg caaaaattaa
     7861 gactactttg caaactgaat ttatagccat tcatcagtat atcaacgaca tttcgaagtt
     7921 gtaactattt tatttgttgc cttctttttt gttcagcaac aattcttttt agtgaatcta
     7981 tttctctagt cattttagct tttgccgctt tagttttagc tttttttatc gaatctgaga
     8041 cttctttttt gcttaacttc ttcttggttt tggacacttc aatgtgcacc ctttttatag
     8101 aatcggattg ttttttttca agtaattttt tctttttttt ggcttttaag atttctttct
     8161 tcgcttcagc tttttgcaaa gatgccaatt gtattttttc cttttccaat cgaactttaa
     8221 ctttttcgaa cattttctga tattctttag aatcagaagc gtaatagata ttgctttgaa
     8281 caaactgaat gctatctatt ttatattttt taaatatata ttggttctga ctatttttta
     8341 aagaatcaaa taagattgga ttatccactt tcatggcttc caacagtgat aaatcataca
     8401 taatatttac catttcctct ctttcaataa gacgttttgg ttccttgacc agctcttttt
     8461 tacagccaac aaggataaac aaaacaataa aaaatggaag tatttttttc atatttaaat
     8521 attatctatc aaatgacaat ctttttccag cacgaatatc ttttacttta gaagcagaat
     8581 acaccatttg tccgttaaca aaagtatgtg taattcgaga tttgaaagta aaaccttcaa
     8641 acggtgacca gccacatttg tatagaatat tttccttttt tacactccaa ggcaaacctg
     8701 aatttacgat gactaaatcg gcataatacc cttctttgat aaaaccacgt ttttctattt
     8761 tgaaaatttt ggctggattg tggcacattt tctccactat tttttcaata ctaattttcc
     8821 cttggtgata ggcttcaaac attgctacaa ctgcgtgttg cactagtggt ccgccagaag
     8881 gtgctttcaa ataagattgt tttttttctt ctaaagtatg aggtgcgtga tctgtggcaa
     8941 tcacatcaat tctaccatcg accaaagcgt cccacaacac ttttcggtca tttgtggttt
     9001 ttactgcagg attccatttt attaaattgc ctttggtcgc ataatcctca ttagaaaacc
     9061 ataaatgatg tacacaaact tcggcagtta ttttcttgtc ttctaatgga attttattag
     9121 taaacaattc catttctttc gctgttgaaa gatgaaaaat atgcaatctt gcaccagttt
     9181 ttttggctaa tgctaaggct tttgaagaag aaataaaaca agcttcttca cttcttataa
     9241 gatgatgtgc cgttactgga atatcttcgc catattcagc tttaaatttt tctaaattgt
     9301 tttttatggt agtttcgtct tcacaatgca ctgcaataag catcggtgta cttgaaaata
     9361 ttttttctaa aaccacttca ttatctacca acatatttcc tgtagaggaa cccaaaaata
     9421 ttttgattcc cgcaacattt ttcgggttgg ttttcaaaac ttcttccaaa ttatcattgg
     9481 ttgcgcccat cataaacgaa taatttgcat atgatttttc ggccgcaatt tgatattttt
     9541 cttctaagat ttcttgagtt acagcatttg gaaccgtgtt gggctgctcg ataaacgatg
     9601 tgattcctcc cgctactgca gctctcgatt ctgactcaat atccccttta tgtgtaagtc
     9661 caggttctct aaaatgaact tgatcatcaa ttgcccctgg aattaaataa tttccttcgg
     9721 catcaataat tttacactca gatgattttg cgctaatact atccgaaatc tcaacaatta
     9781 agtcgttttc tattaataca tctccttcaa aaatagttcc ctcgttaact attttagcat
     9841 ttttaattaa aaccttattc atcgtcattt ccttttataa ggtgttaaat aattttttta
     9901 aacgtagtgc aattactcct aaaacagctt ctttaaaaat ggcactgctc atcttagatt
     9961 gtcctttagt tctatcggta aaaataatgg ggacttcagt gatatcaaat tttttgcaaa
    10021 aagttctata tttcatttca atttgaaaag cgtatcccac aaattttatt ttgtctaaat
    10081 ttatcccaac caaaacctca cttttatagc atacgaatcc agctgtagca tcgtgaattt
    10141 tcattccagt aataaatcgc acataaaccg atgcaaaata tgacattaaa acccgactta
    10201 atggccagtt taccacattt actcctgtta cataacgaga accaatagct aaatcggcac
    10261 ctccaaaatg gcaagcatca tacaatctct ctaaatcttt tggattatgc gaaaaatcgg
    10321 cgtccatttc aaaaatatag ctataattat gctccaaagc ccatttaaaa ccgtgaacat
    10381 atgcggttcc caaacccaat tttcctgttc gtttttccaa aaacaatctc ccttcaaatt
    10441 ccgtttgaag taaagttact ttttccgcgg tatgatctgg cgaattgtca tcaataacaa
    10501 ggacatgaaa cggtttatgc tgggaaagca ctgccctaag tatgctttca atattctcaa
    10561 tttcgttata ggttggaatt ataacaatac aatcattcat atcttgcgta atttcaccgc
    10621 aaaagtaaac tttttatacg atttgactcc tatctgaatt caaataattg tttataaaat
    10681 aatataacag ctgtattctt cttttatatt tacgattttc aatcaggaca tttttttaat
    10741 acttttttaa tctaaataat gaaataaaaa attagtaatt ttgcagcaat atgattgata
    10801 atgtacttca tttaagggta atcgagaaca aagattgggc aacaatcttg tttgtacttt
    10861 catttgccac tgttgcaata acaaaatcgg catttgaaaa tcgttttgct gaatttgcaa
    10921 aattaattta ctccgacaaa tacacgaatg tttataaaga gggcagccac cttaaaagca
    10981 gttttacaat agctttattt tttgtccaac tcatctcatt tgcttttttt attcaacttt
    11041 cgttttctta ttttggctat agttcaaaaa cagattgggt tttatttatt caagtaatta
    11101 cgtttctaat ttatttcgtt ttaagtaaat ttttaatcga aaaaattatt gcaaccgcct
    11161 tcaatattga ggaaatcatt gaacaattca accttcaaaa ggtaattttc agaacctaca
    11221 tagggttatt cttattgcca gtcaacataa tcttgttcta caacaatgct atttcaagaa
    11281 attttatgat catacttatt tctatagtgc tgatttttaa tgtgataata tatttagtat
    11341 caataaaaaa ttatcaaaat ataatattta gcaagttgtt ttattttatt ttatatcttt
    11401 gcgctctcga aatagcaccg tattatttct tatattattg gtttacaaaa ggtattgctt
    11461 agaaaatgtc aaatatgaaa gtgaaaacaa ttttggtgtc acagcccgaa cctaaagtgg
    11521 aaaactcccc ttactttgaa cttcaaaata agcataaagt aaaagttgac ttcaggcctt
    11581 ttatccacgt tgaaggagtt agtgccaaag aaattagact ccaaaagatt gatcttaacc
    11641 attttactgc aattatttta accagcaaaa acgctgtaga tcactttttc agagtggcag
    11701 acgaaatgcg ttataaagtt cccgaaggat tgaaatactt ttgtcaatca gaagcgattg
    11761 cattttacct tcaaaaatat gtagtctata gaaaacgtaa aatttacgtt ggcccaaaag
    11821 attttgccga tttatctcca ctaataaaaa aatacaaaga cgagaaattt ttgctacccg
    11881 cttcagacca actgaatgca gatgctccaa taactcttaa tgctttgaaa gtagattgga
    11941 caccagctgt tttttacaaa acagtaatta gtgatttgtc tgatttagcc gatgtttatt
    12001 atgacgtttt ggctttcttt agcccaactg gaataaaatc attgttcact aattttcctg
    12061 attttgagca aaacaatact agaatagctg tttttggaag tacaactcaa aaagcagctc
    12121 ttgaaaaagg gttgagaatt gatattcttg ctccaacacc agaaacacct tcgatgacaa
    12181 tggcattaga aaaatatatt tcagaagcga ataagggaaa ataaacttat actgaaactc
    12241 aacatttaaa gttccaaact ccaattttac cattggaatt tggaactttt tatttggaaa
    12301 atgtttgttt ttaaatcatt tcaactactt ttgatataaa ccaaaaacta aaactatgaa
    12361 aaaaatccca ttaattattc ttatgatctc tttatccttg gtaattgtta attgtagctc
    12421 ttctaaaaaa ggaacagcag atcccaaaat cagttacaaa acagatatag cacctattat
    12481 ttcgaatagt tgcactccgt gccacatgcc acctcagggt cgaaaagaac ctttagaaaa
    12541 ctacattcac gtcaaagaaa acattgtcga aatcttggaa cgtgtgcaac taccacaaga
    12601 caatcgcaaa tttatgcctc ctgcaaacaa aaaacccgct ttgacaactg atcaaatagc
    12661 acttttagta aaatggcaac aacaaaatat gcctgaataa aaaaatattt atgaaataaa
    12721 aaacggctgt ttcacactgg aaacagccgt ttttgttttt tgttattgcg atgaaccgaa
    12781 gtcatatcac attatgcaat ttatcttcta ttcatataaa tagaaacata atacaataaa
    12841 gtcgcaatcg aaccaagcgc agcaactaca taggttcttg cagcccattt caaagcatct
    12901 ttcgctcctg cttgttcttg ttgtgtcagc attcgtttgt tttctaacca agccaaagca
    12961 cgattactag cgtcgtattc tactggcaaa gtcacaatcg aaaataaagt tgttgcagca
    13021 aaaatcacaa ttccaatcaa taacaactga ggaaaagttc taatcatcag gattcctgcc
    13081 aacaaaatcc attgcatata agacgacgcc acattaacaa ttggaaccaa ttgcgaacgc
    13141 attgtcagcc attgataccc cacagcgtgt tgtacggcgt gaccacattc gtgagccgca
    13201 accgcagctg ccgcagcact cctttgattg taaactgctt cgcttaaatt cactgttttg
    13261 tccacaggat tgtaatgatc ggtcaattga cccggagtcg aaataacacg aacatctcta
    13321 attccgttat ccgccaacat tttttcggct atttcggcac ccgacattcc gttttgcaaa
    13381 tgcaatttag agtattgttc gaatttactt tttaatcttg aactcactaa ccaactggcc
    13441 agcataatca atcctgctaa aagcatatat cccattccca taattttatt ttttaattgt
    13501 ttgaaattaa accatcaaat tgtgaaccaa atttagaaaa tgacaatttg acatctgtta
    13561 aaaaagtttt aaaaaatcaa tggcaatttc aatggcaatt tcaataatgg gtagtaataa
    13621 aaaaaatcta aattccaatt aaaaagattc ctacggaatg acaaactacg attgataatc
    13681 attcaaacaa aatctcatct tcttgtcatt ccgttggata tgagcgataa cgaactggcg
    13741 gagcaatctc aaactaggag caaaaaaaaa tccaaactcc aattaacaca atgtcattga
    13801 aatttggatt cttaaaatat ttaggttagt tgcatttaca attcacaact taccattcac
    13861 cattaattta ccccaccaag ttgataattt ttcccggaac gataatcact ttgtttggtg
    13921 tttttccgtc cagttgtttt tgggttcttt cgtctttcat tacgatttcc tcaatttgtt
    13981 ctttggttaa atccaaaggc aaggtgatag taaaacgcat ttttccgttg aaagaaaccg
    14041 gatattcttt ctccgattct accaaatgct ttgcttcaaa aactggaaaa tcaactgtcg
    14101 aaattgaccc tgaatttcct aattgagccc acaattcttc agcgatgtgt ggtgcataag
    14161 gcgaaatcaa aatcgctaat ggttctaaaa tcgcacggga atgacaattt tgagttgaca
    14221 attcattcac gcaaatcata aactgcgaaa ccgaagtgtt gaaagagaaa ccttcaatat
    14281 cttctgctac ttttttgatg gttttgtgca atgattttaa gttgtctttg gttggtgtgt
    14341 cgttggttac gattgaacca ttttcgtcaa aatacaaacg ccataatttt ttaagaaaac
    14401 caaaaactcc tgaaattcct gccgtgttcc aaggtttagc ttgttctaat ggacctaaaa
    14461 acatttcata caaacgcaac gtatcggctc cgtattcatt acaaatatca tctggagtta
    14521 ccacattgta tttcgatttc gacatttttt cgatttcacg accaacaatg tattttccat
    14581 tttcttcggt aataaattct gcatttgcat aatcttctct ccaagatttg aatttttcaa
    14641 tatctaattc atcggaagag tttacaaaag aaacgtcagc gtgaatatta tttatttcaa
    14701 ttttatcaat atcaataaac tctacaactt cttttgaatc ttcaacatta tattttttct
    14761 taattgcatc cgaaaaatat ttctttaaaa ttattgaatc accctcaata attttttttg
    14821 actctccatt tgaaatgaat aaatttttaa aaatttcact atcatcatta ttttcataac
    14881 cttttcgata tgaaataaca tatgaattag gcaagtctat acgttttaca aacgcactca
    14941 ttcccaaaat cattccctga ttaatcagtt ttttgaatgg ttcttcggtt ggtgcaaaac
    15001 ctttatcttt taggaatttg ttccaaaaac gggaatacaa taagtgaccg gttgcgtgtt
    15061 cacttccgcc aatgtataaa tccacacttt cccaatattt caaagcctct ttgctcgcaa
    15121 attctccgtc attttttgca tccatataac gcatccaata ccacgaactt cccgcccaac
    15181 ccggcattgt gtttaattct aatggaaaaa tagtcgcgtt gtcaaccaaa ttcgtatcaa
    15241 ccactttatt atttacactg tcccaagccc aaacagaagc atttcctaat ggaggcaatc
    15301 cgtcttcggt tggcaagtat ttctccactt ccggcaacac gattggcaaa tgttttgcat
    15361 caatcatttg tggcaagcca ttcacgtaat ataccggaaa cggttcgccc caatatcttt
    15421 ggcgggagaa aaccgcatca cgcaaacggt aattgatttt tccttttccc tgacctattt
    15481 tttctaattc tgaaatgact ttcttcgttc cttctttgta tcctaatcca ttcaagaaat
    15541 cagaatctac caattgaaaa ccttctttgg ctccaaaagc cgattccgaa atatctttat
    15601 caaaaatatt tttgatgtcc tgcattccat tttgcccttt gaagaaattc gcgaaagcat
    15661 aatctctttc atcaccgcaa ggaaccgcca tcacagcgcc tgttccgtaa cccgccaaaa
    15721 cataatcccc aatccaaacc ggaatcggtt ctttggtaaa cggatgctct gcataagctc
    15781 ccgtgaaaac tcccgaaatg gtttttacat ccgccatacg ttctctttcg gaacgtttag
    15841 cggttttttc tatataagct tcaacttctg ctttttgctc agcagtcgtg atttgtgcca
    15901 ccaattcgtg ttctggagcc aaagtcataa aggtaactcc gaaaatggtg tcgggacggg
    15961 ttgtgaatac gtcaataaac ctcacccccg acccctctcc agaggagagg ggagcgagct
    16021 cctcgtttat attatatgaa tttcgcagac ttaattcttc tttaatcttc gttaaaacag
    16081 atttgatgtc gtttaaaact tcttcattag taaagcgaat aactttataa agatgctttt
    16141 gctctaattc taaagttcgt tgttcatcta attctatttg ttctttcgta tcgtgatatc
    16201 ctccgtcaac ttcaataatt aactttttat caagagaaac aaaatcaaca ataaaatttc
    16261 caatataatg ctgtcttctt attttgtaac ctaaattcga atttctgact tcttgccaaa
    16321 gtgcatcttc agcctcagtt gaattcttgc gattttccct tgcattacca accaattgcg
    16381 aagcaattga agcattagct gtcatatatc caggagcttg gctcccctct cctttggaga
    16441 ggggctgggg gtgaggtttt actgcaaaag aaaccatcgc tccaaccgat tttccgatcc
    16501 agtttctttg tgattctttt attgattcgc tccagtcaat cgtgtccaaa ccttgcaaca
    16561 aacgttctgc ataagcagaa attcgcatac tccattgggt cattttcttg cgaataacag
    16621 gaaaaccacc acgttctgaa acgccgttta cgatttcgtc attcgccaaa acagttccca
    16681 atcccggaca ccagttcact tcggtttcgg ctaaataggt taatctatat tgtaatagta
    16741 ttttttgttg ctcttcggat gaaaatgcgt tccattcgct tgacgaaaaa ccaacaatat
    16801 tatcgtcaca aactgcattt acattcgcat ttccttcttt ttcgaaaacc gaaaccaaag
    16861 tcgaaatgtc ttcagctttg tcggtatttt tattgtacca ggaattgaat aactgaataa
    16921 aaatccactg cgtgtgtttg taataatcag gattcgaagt acgtacttca cggctccaat
    16981 cgaatgaaaa tccaatttta tccaattgtt tacggtaacc cgcaattttg tttccttctt
    17041 tgtccacacc tccgtcaata tttaccgaag tcgtgtcttc cggacgctga cctgtttgaa
    17101 tcgcatattg ttcggccggc aatccgaaac tgtcgtaacc cattgggtgc aatacattaa
    17161 aaccttggtg tcttttgaaa cgagaataca cgtccgaagc aatgtaaccc agcggatgcc
    17221 ccacgtgcaa tcccgctcca gacggataag gaaacatatc caaaacataa tattttggtt
    17281 tttcggagtt gttttcagct gaaaaagttt ggttttctgc ccaatgtttt tgccatttgg
    17341 cttctatttc gttcggattg tatttcattt gtattgatat gatttaaagt cacaaagact
    17401 ttctgaaaaa agtttatttt gagatgccaa atttacattt attgtacgaa aactgaaagt
    17461 ttgagcgtta ttaactttaa ataaagaaat tgtttgttat ttttacccca taattcgaat
    17521 aaactatggc ttctaacttt gataaatttc aaaaacgcag attactttcc tcctattttt
    17581 cagttgtact aagtgtattc ttagttttat tccttttagg aatactaggg tttttcatta
    17641 taaattctag gaaattggca aacaatttta gagaagaaat tgctatgact gtatttttta
    17701 agaacgatgc gaacgacaca attctaaaag cttttgccaa agaattgaaa gtagctaaat
    17761 tcactaaatc gtttgtctat gtttcaaaag aaaaagcagc aaaggaacat accgatatta
    17821 ttggcgaaga ttttatgact tttttaggta caaatccttt gcaaaactct tatgacattc
    17881 acctgaaagc cgattatgta gaaagagata gcatagctaa aattgaaagt cttttgcaca
    17941 aaaatcctat ggtttcagac attgtttatg acaaacaatt agtgaactta gtgaatgaca
    18001 atatcaaaaa agtgagtatg tggattttaa taatcagtat tttcttaacg tttgttgcgg
    18061 ttttattgat taatagctct ttgcgtttat ccatttattc tcatcgattt attatcaaaa
    18121 cgatgcaaat ggttggtgct acaaaagcat ttatcagaaa accatttgtc atgcgaagca
    18181 taaaacttgg aatgcttgga gcaggattag caattattgc tctaattggt gttttaattt
    18241 atattcaaac taattttcca ggtttaggaa ttcttgacga taaattactg gttgcacttg
    18301 tattgttatt agtttttggc gtaggtgttt tgatcacttg gataagtacc tattttgcaa
    18361 cacaacgctt tttgaattta agaacagacg atttatatta gtcatccgtt ttcagtaaaa
    18421 aacagaaata aaaatgaaaa acaaagaaga acaaaaacac gaatttcttt ttgagaaaac
    18481 aaactacatc atactactta taggtattgc cgtaattgca cttggattta tattaatgtc
    18541 tggaggcgga agcgatgacc ccaatgtatt taacgaagaa atatttaatt ttagaaggat
    18601 tagattggcg ccaacaaccg ttttaattgg ttttgggatt actatttatg ccattcttaa
    18661 aaaccctaaa aaatagttgg cagctttcag tgttcaatta gcacaaataa aatctaaaat
    18721 ctacactcta aatctaaaat caattgaata catttcaagc tattattatt gcaattattg
    18781 aaggaattac cgagtttttg cccgtttctt caacgggaca tatgattctt gcctcttctt
    18841 ttttcggaat tgagcacgat gatttcacca agctttttac gattgtcatc caattgggtg
    18901 caatcctttc ggtagtcgtt ttatatttca aacgcttttt tcaatcgttt gatttttatt
    18961 tcaaattatt ggttgctttt attcctgcgg tagttttcgg attacttttc agtaaaaaaa
    19021 tagacgcatt actagagagc cctttaacag tagctgtttc actattgata ggagggatta
    19081 tcttattgaa agtggacgat tggtttacca atgctgaaga agtacaaaca tcaaatgaaa
    19141 ttagttatgc tcaagcatta aaaattggtt tgtttcaatg tttggctatg gttccaggag
    19201 tttccagaag tggcgcatct attgttggag gaatgtcaca aaaattatca cgcacatctg
    19261 ctgccgaatt ttcattcttt ttagcggttc caacgatgct tggagcaaca gccaaaaaat
    19321 gttatgatta ttacaaagat ggacttgttc tatctcacga ccaaatcaat tttttgatta
    19381 ttggaaacct agttgccttt attgtggcac ttattgccat taaatctttt atcgggtttt
    19441 tgactaaaaa cgggttcaaa gtatttggtt attaccgaat aattgctgga attctacttt
    19501 tagcaattca ctttttcatc catcctttga caataattta atggtatctg ccgaagattt
    19561 cttgaatgga caaatcattt taattgacaa gcctttgcat tggacttctt ttcaggctgt
    19621 taacaaaatg aaatatgcgt taattaataa agttggactt cctaaaaatt taaaattggt
    19681 catgcaggaa ccttagaccc tttggcaagt ggattattat tagtttgcac tggaaaattt
    19741 accaaaaaaa ttaccgaact tcaaggtcaa gccaaagaat atacgggaac tttctacatt
    19801 ggggcaacca caccttccta tgatttagaa accgaaatag atcaaacttt cgacagttcg
    19861 cacatagatg aaaaattgat ttacgaaacc gtaaatcaat ttttaggaga aatagatcaa
    19921 aaaccaccta tattttcagc tataaaaaaa gaaggcgtac gtttgtacga acatgcacgt
    19981 gcaggagaaa ctgttgaaat tgcaagtcga aaaaccacta ttcatgaatt cgaaatcact
    20041 cgaattgcac ttcctgaaat cgactttaga gtggtgtgca gtaaaggaac ttacattcgc
    20101 tctttggctt atgattttgg catagcaatg aaaacaggct cacacttgac ggtgttgcgc
    20161 agaacaaaaa ttggtgatta cgacgtaaac aacgccgtgg acatcacaat gttcgaacaa
    20221 aatttattac aataaaatac actccaaaat aaattttaga gtgtatattt ataaaatcaa
    20281 agtattttta taccatactt tgatttttat agaagatcaa tttacgccat tttattaata
    20341 atggctggtg gtttcacctc caaacaataa ggttgaaatg taattgtaaa atttgtcaaa
    20401 taaagttttc atggcgtgtt tttttattgt taaacaacat ttaaacacca caaaaaagta
    20461 aattgtaata gtagatttgg ttgagaccgc taaattacaa ttttttttta aataaaaaaa
    20521 attattccta tcatacaaaa ataaatagaa ctcaaattat tttttttaaa aaacagcatt
    20581 ttaaaaaaac agattatgcc tatatttgca caacataact tgaactaatt taactagttt
    20641 tttggaacat tatgtgtcca atttttaaat ataaaacgga atgaaatata aaagaatcct
    20701 tctaaaattg agtggcgaag ctttaatggg cgaaagacaa tatggtattg accctgcaag
    20761 attaggtgaa tatgctgatg aaattaaaaa aattcacact aaaggtgtgg aaattgcgat
    20821 tgtaattggt ggtggaaata tttttagagg agttgctgga gcaagcaatg gtatggatcg
    20881 agttcaaggc gattatatgg gaatgttagc aaccgtaatt aatggcatgg ctttacaagg
    20941 agcccttgaa gacaaaggaa tgaaaactcg tttgcaaact gccttgaaaa tggaatctat
    21001 tgcagaacct tatatcaaaa gaagagcaga tcgtcatctt gaaaaaggaa gaattgtgat
    21061 ttttggtgcc ggaacaggaa atccgtattt cacaaccgat actgctgccg ttttgcgtgg
    21121 agtagaaatt aacgccgatg tgattctaaa aggaactcgt gttgacggtg tttataccgc
    21181 cgatccagaa aaagatgcca ctgcaaccaa atttaactac atcacttttg atgatgtttt
    21241 gaaaaaagga ttgaatgtta tggacacaac tgccttcacc ttgagtcaag aaaacaaact
    21301 acctatagtt atttttgaca tgaacaaaca agggaatttg ttgaaaattt gtgaaggaga
    21361 aaacgtagga acagaagtaa atatatagtt tatggtttat ggtttgttgt ttattgttaa
    21421 ctacaaacca taaacaacaa gcaacaaaca ataaaattat gactgaagaa attgaattta
    21481 tactagacag cactcaggaa tctatggagg gcactattgc gcatttagaa aaatctttct
    21541 tgaatattcg tgctggaaag gcttcacctg caatgttagg aagcgttttt gtagattatt
    21601 atggatcggc ctcaccgctt tctcaagtgt caaaaattag tgttccagat gcaagaacca
    21661 ttacacttca accttttgaa aagaatatgc ttcatccaat tgaaaaagca atcatgatag
    21721 caaatcttgg tttcaatcca atgaataacg gagatgtaat catcattagc gttccgcctt
    21781 tgacagaaga ccgcagacgt gaattggcaa aacaagcaaa agccgaagcc gaagatgcca
    21841 aaattgggat tagaaatgcg cgtaaagacg ctaataccga aataaaaaaa ctagaaaaag
    21901 acggaacttc tgaagatatt tgtaaaaaag ccgaagaaga agttcaaaat ttgaccaata
    21961 gttttatcaa aaaaattgac gaacatcttg ttcataaaga agccgaaatt atgaaggttt
    22021 aaaatctctc attttagtaa ataaaaaatc cgctttgagc ggatttttta tttactaatg
    22081 ttttaaaatc aaaactgaag gaactcttct cagccaaaca ctatatttat ctaaaagtac
    22141 tttccaactt tcgaccccga taatcatcaa atctaaatgc tctaataaat cttttttgat
    22201 gctgttttca tctattaaaa taatgttttt ggggtatttt tgttctaaag aattgatggc
    22261 acttttaagt acaaaattgt cattactatt tgcatttaca tccaaaatct taattttcga
    22321 attgttgtta gtaattaatt tttgagcata gtctaataaa aaagcatcct cagaactaaa
    22381 aattggaata ataactcgat ctacttgttg caaatcttta tcgattaaaa tacccaaagg
    22441 ggttttagtt tttgcaatta tttgtcgagt cctttcatca aaaggagaat tttcgaaaag
    22501 accttctttt cccgtaaatt tatcaatcaa acgatcggga ttaattattc ttgtggtaaa
    22561 ccctaatact tttcctagta gagaaccttc aaaaatagat tttccaagcc caatcaaaag
    22621 taaatcatag tctccattat tagcaacctc agcaatatta gattcaatat ctacagttgc
    22681 ctgaaaaaga gttgtaattt cttgattcaa ggtctttgac tcttcaatta ttggcaaaaa
    22741 tttatctttt tcgtattctt ctaaattata agcatgcact tcgtcactta atgtaagatg
    22801 catggctgtt atggctgcct tttcattttg ttttttgact aagctattgg ctagcctcaa
    22861 gagcgacttc cctttttcat tatttccaaa agaaatcaaa attttatact ttttgatatt
    22921 cgcaatagaa atttcttcta aatcagcatc gtcttttgtt ttgaaaatat aatttataaa
    22981 attcaatgtc ggccctgtca taaaagtagt aactagtgcc attataacca tcatcgtaaa
    23041 aacttcggga gtaagcactt tcaaatctag gccaatattc aaaactatca actccatcaa
    23101 ccctcttgtg ttcatcaagg caccaatagt gaaactgtca cgccaacttt gtcctacgaa
    23161 tttggctgcc aaagcacttc ccaaaaactt accaattacc gcaacaagga taataaaacc
    23221 tgtaactctc cataaaaaag gatcgtttat caatcctatt tctgttcgca aacctgtaaa
    23281 tacaaagaac aaaggaagca acaatatcaa cgaaacatct tcaacttttt caataaatag
    23341 atttctaaat ttggttatgt caggcataat aactcctgtc ataaaagcac caaaaagtgc
    23401 gtgaatccca ataatttcag ttgtatatga cgaaataatc aaggttaaaa agaaaatggc
    23461 taccactggt ttgtttatac tatcctttgt tgcatataac tccccaattt tagctaaaaa
    23521 tggttttaca acaaaaagca tagctaaaac ataaaccatt gccattccaa taatatagag
    23581 cgaacttaca aaggttccag cttttacaat ggcaataact gcagcaagaa tgcaccaagc
    23641 cgtaatatca tcggcagcag cacaagtaat tactaccgcc cctaatttag tcttattaat
    23701 tcctctttct tgaacaattc ttgctaaaac tggaaaagcg gtaatactca tagcaatccc
    23761 cataaacaag gcaaaaggta agaaagcaac ctcttctggt gcaaatctaa aatacacaaa
    23821 ataggccaag ccgataccca atgcaaaagg aattacaata ctagcatgac taataacaac
    23881 agcgtctttg gctctatttt gcaaaacttt caagtcaagt tccattccaa tgacaaacat
    23941 aaaaagtatc agtccaatct ggcttaaaaa ctgcaaattt cctaatgaat caacaggaaa
    24001 taaagctaat gaaaattcag gaaaatacaa acccaataag gatggaccaa gaactattcc
    24061 tgcaataatt tcgccaatta cagagggttg accaattttt ctaaaaaccc atccaaaaaa
    24121 acgagcgaca attattatag ttacaatttg tgccaaaagt atggccagag gatggtgtac
    24181 gttaagcgag attgaatcaa caaaatcatt ccaatgccca ttttcagtta caggacgcaa
    24241 gtatttctta tgaacttcca gaccttttcc catagtcaaa atccaatata ttaaagccga
    24301 aaaaccgcct gtaacaacga gataaaatat agtatttctg taatttttca taagttttgc
    24361 ttcaatattt ttcgtacaaa gatgcagaat tcaaatatat aaattacatc tcattaaatg
    24421 aatttattgt aaaataaaaa caaaaaatta acaaaaagag ggtttaaaaa tcaataatat
    24481 aataagttga ttaactacct ttgcagcatt taaaattatg taatgatcaa gtggtttagt
    24541 ttgggatttt gggaattaat tgcccgtgtt gtccttcgaa atagaatttt aatgttgtct
    24601 ttggttattc tgacaacagt tttgctggca atgcaatgga aaaacatcca tttcactcaa
    24661 acagaagcca acatgttacc cgacgacaac attgtcaata ttgagtataa tgctttttta
    24721 aacaggtttg gcgaagaagg aaacttggtc ataattggcg taaaagacaa aacttttttt
    24781 accccaaagg cttatgccgc ttgg
//
LOCUS       DAOSNN010000009        23936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_2130_length_23936_cov_5.178217, whole genome shotgun sequence.
ACCESSION   DAOSNN010000009 DAOSNN010000000
VERSION     DAOSNN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 23936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23936
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2130_length_23936_cov_5.178217"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLQ70_00970"
     CDS             complement(<1..385)
                     /locus_tag="PLQ70_00970"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HQE33216.1"
                     /translation="MSLYLFLNIASFIIPFLYSFENKMRYIKRWKAVFSAILITATFF
                     IIWDVFFTKIGIWSFNPRYHSGIEFFDLPIEEWLFFICIPYASLFIHFAFHYFCPKVS
                     LTDRTVKLIYWILMLITIPILFFNID"
     gene            complement(450..890)
                     /locus_tag="PLQ70_00975"
     CDS             complement(450..890)
                     /locus_tag="PLQ70_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQE33217.1"
                     /translation="MMSFFIFLTTYLLMECVTWLTHKYVMHGFLWYLHEDHHQPKYAH
                     IFECNDLFFVIFAIPSILLFYFGADAGFDYRFFIGLGIFAYGLSYFIVHDIIIHQRLK
                     LFKNTKNKYLIALRKGHKVHHKHLGKEEGECFGMLFVPFKYFKI"
     gene            complement(919..1758)
                     /locus_tag="PLQ70_00980"
     CDS             complement(919..1758)
                     /locus_tag="PLQ70_00980"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HQE33218.1"
                     /translation="MKNLFDEVSFKCSVLVTKSYSTSFSLAVKMLAPSIREAIYSIYG
                     FVRFADEIVDSFHGYDKETLINDFEIEYYKTQKMGISLNPILNSFQQTVKKYNIHDDL
                     VQSFLKSMKMDLVKSDYHTKQEYDEYIYGSADVVGLMCLKVFVKGDNQKYEELKDEAM
                     RLGSAFQKVNFLRDLKDDNLVLNRNYFPGVDLNSFDEKSKIMIVNEIEEDFRVALQGI
                     KKLPLEAKFGVYTAYVYYKKLLRKLEKTPSHKIGTERIRVSNYTKGLLFANSFVSYKL
                     NLV"
     gene            complement(1770..>2129)
                     /locus_tag="PLQ70_00985"
     CDS             complement(1770..>2129)
                     /locus_tag="PLQ70_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HQE33219.1"
                     /translation="GIFLIPIAPGIEDTPEIREHYFKSIIERFEQLTNQTISENIIFK
                     ESFCVKDFVKDYNSYKGNAYGLANILTQTAFLRPKIISKKVKNLFFTGQLTVPGPGVP
                     PSIISGKIVSDLIKKQF"
     assembly_gap    2130..2229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2230..3056)
                     /locus_tag="PLQ70_00990"
     CDS             complement(<2230..3056)
                     /locus_tag="PLQ70_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQE33220.1"
                     /translation="MRKTITIIGSGFSALAASCYLAKDGFDVTILEKNNTVGGRARQL
                     SKQGFTFDIGPTWYWMPDVFEKFFADFGKIPSDYYHLDKLNPAYEVYFGKADSVQIPD
                     NLPDIFNIFEKEEKGSAKHLESFLDNAKHNYDVAIKDLVYRPGISITELITPVTIKKV
                     NQFFSTIRTSVRKKIKNNRLQQIMEFPVLFLGAKPSNTPSFYSFMNYADFGLGTWHPK
                     GGMYEVVKAMVTLAVELGVKIETNQNVEKINVENGVVKSVVSNGITIESDVVLSGAD"
     gene            complement(3112..4008)
                     /locus_tag="PLQ70_00995"
     CDS             complement(3112..4008)
                     /locus_tag="PLQ70_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQE33221.1"
                     /translation="MNNIKSVFSIKDLENLSGIKAHTIRIWEKRYNILEPMRSNTNIR
                     NYDTHNLQKLLNIVLLHNYGYKISRIAEHSSEKIELLVREIISEKSTKNHAINAFKMA
                     MMNFDQVLFFKTYSNLLSEKSFRDVFYEVFIPLMNEIGLLWQSGTITPAQEHFISYLI
                     KQKLLVNIEKIQVLEPTRNDKVFVLYLPENEIHELGLMYLNYEILLNGYKTIYLGESV
                     PIESLKDMKQYYDNIVYVAYLTVQPTKDEVNTYLQEIATEIMTADSQVWLLGRMTECI
                     DLSKLPKAVSVFNSIPDLVKEL"
     gene            complement(4057..5016)
                     /locus_tag="PLQ70_01000"
     CDS             complement(4057..5016)
                     /locus_tag="PLQ70_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HQE33222.1"
                     /translation="MALLKRINEKAKSYLSSGFGVNTSSYGGRFFNKNGKANVEKQGM
                     HFLHRISWYHTMIEMPAWKFMSILLSFYVGINLVFALIYFAIGIEYLNGIDTSASEWV
                     KFGETYFFSAQTFTTVGYGHVSPSGFLASSVSALEALIGLLSFAIATGLFFGRFSKPV
                     AFIKFSHNAIIAPYRDGTALMLRLTPFKNTNFTDAEAKIMLGMSVEEAGIVSNKFYSL
                     DLELDKINALTLSWTLVHPITEDSPLYHFTKEDFKTINGEILVFIKTFDDMFSNTVAI
                     RTSYTFDEVVYGAKFLPMYSRNSSNTKTVLYLDKLNHYEEVVL"
     gene            5170..6060
                     /locus_tag="PLQ70_01005"
     CDS             5170..6060
                     /locus_tag="PLQ70_01005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQE33223.1"
                     /translation="MKKLTKEDISQEVFDLYDDYAHNKIERRQFIEKLSIFAVGSLTL
                     PSLLSFMTPNYVDTITIKADDPRLKSEFITYESPKGGSIKGLLSQPTETKKKLPGIIV
                     VHENRGLNPYIEDVGRRAALEGFITLAPDALSPLGGYPGNDDAGRELQKKRTREEMLE
                     DFIAAYEYLKSHKDCNGSVGVVGFCFGGWISNMMAVKIPTLSAAVPYYGGQPTAEDAE
                     KITTPLLLNYAGLDTRVNEGWPAFEAILQKNKVEHTAYFYEGINHGFHNNTTPRYDEK
                     AATLSWTRTIDFFKEKLKKK"
     gene            6166..7218
                     /locus_tag="PLQ70_01010"
     CDS             6166..7218
                     /locus_tag="PLQ70_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33224.1"
                     /translation="MKLNIILLYLLFSNLWLYSQKKPIENNSKEFLAIDFNGCVKSIA
                     IKSYSYNKKKETIDTTASYYKIFFAKNGNIIKHIYFDKSFTIEKKIIEYDELERIKTI
                     KINDDGKIWIASEQFFNNFSNYPDSTNIYYYGNKYKEKYINLFDKKNLIKQEFYSQDT
                     LRHYNTYIYDKKNRLIKDYFTNTQNGFGITLGKSITGDKDEKTLWPNDSTIYDYKKIK
                     DTLITIKLEQKGWKEIKKELKSNIFTLEILEKYNRNFLENSRYTYKSKDSIFDCTYYY
                     KEKREVKSFYKTITTPKSIVSNWKSDGFYNNEARTETTYIEIEFDAYNNWIEKKYSKD
                     NATISLITREIEYYYH"
     gene            complement(7279..10134)
                     /gene="carB"
                     /locus_tag="PLQ70_01015"
     CDS             complement(7279..10134)
                     /gene="carB"
                     /locus_tag="PLQ70_01015"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQE33225.1"
                     /translation="MPKDNSIKSVLIIGSGPIVIGQACEFDYAGSQSARSIREEGIEV
                     ILINSNPATIMTDPSMADHIYLKPLTTKSIIEILKAHPQIDAVLPTMGGQTALNLCLE
                     ADEKGIWQDFGVKMIGVDVNAINITEDREQFKQLLQKINVPTAPAKTATSFLEGKEIA
                     QEFGFPLVIRPSFTLGGTGAAFVHTKEEFDEKLTYGLEMSPIHEVLIDKALIGWKEYE
                     LELLRDKNDNVVIICSIENMDPMGIHTGDSITVAPAMTLSDTTFQKLRDMAILMMRSI
                     GNFAGGCNVQFAVSPDEKEDIVAIEINPRVSRSSALASKATGYPIAKIASKLALGYNL
                     DELQNQITKSTSALFEPTLDYVIVKIPRWNFDKFEGADRTLGLQMKSVGEVMGIGRSF
                     QEALHKATQSLEIKRNGLGADGKGYTNYEQIIEKLTFASWDRVFVIYDAIAMGIPLSR
                     IHEITKIDMWFLKQYEQLYILEKEISTYTVANLPKELLLEAKQKGFADRQIAHMMNCK
                     ESEVHTLRMTMNINRVFKLVDTCAAEFTAKTPYYYSTFEAEIEKANGERYVHNESIVT
                     DKKKIVVLGSGPNRIGQGIEFDYSCVHGVLAAKECGYETIMINCNPETVSTDFDTADK
                     LYFEPVFWEHIYDIIQHEKPEGVIVQLGGQTALKLAEKLSKYGVKIIGTSFDALDLAE
                     DRGRFSDLLTELNIPFPKFGIAETADEASALADTLDFPLLIRPSYVLGGQGMKIVINK
                     KELEEHVINLLKSIPGNKLLLDHYLAGAIEAEADAICDADGNVYIIGIMEHIEPCGVH
                     SGDSNATLPPFNLGEFVMQQIKDHTFKIAKALKTVGLINIQFAIKDDTVYIIEANPRA
                     SRTVPFIAKAYGEPYVNYATKVMLGEKKVTDFDFNPKLNGYAIKQPVFSFSKFQNVNK
                     ALGPEMKSTGESILFIDDLKDDQFYELYSRRKMYLSK"
     gene            complement(10289..10450)
                     /gene="rpmH"
                     /locus_tag="PLQ70_01020"
     CDS             complement(10289..10450)
                     /gene="rpmH"
                     /locus_tag="PLQ70_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQE33226.1"
                     /translation="MSKRTFQPSKRKRRNKHGFMDRMASANGRKVLARRRAKGRHKLT
                     VSSEPRHKK"
     gene            10615..11130
                     /locus_tag="PLQ70_01025"
     CDS             10615..11130
                     /locus_tag="PLQ70_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQE33227.1"
                     /translation="MFHKNIKLIITVLLVVTAIWQFTESNIGNGIFLILLTAFPIFLY
                     FKNEFILLAFLKLRKQDFDGAKKWLDYIKNPEGALVRKQQGYFNYLHGIMLSQTNINQ
                     SEKYFKKAIELGLSMDMDLAVAKLNLAGIAMSRRRKLEATNLLNEAKKLDKQNMLTDQ
                     IKMMKEQMKKL"
     gene            complement(11199..11855)
                     /locus_tag="PLQ70_01030"
     CDS             complement(11199..11855)
                     /locus_tag="PLQ70_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQE33228.1"
                     /translation="MNKIPVYFMPGLAASSSIFERIVLPDDVFEMILLEWEIPLDNET
                     LPQYAKRIADKITHSSPVLIGVSFGGILVQEMAKFITARKVIIISSVKTNIEFPKRMK
                     MAKTAKAYKLIPTTLVANIESLSTFSFGKTINQRLKLYEKFLCVRDKHYLDWAIEQII
                     LWQRTVADENVVHIHGDADDVFPIKNIKNCIVIKGGTHIMILTKYKWLNENLPRIILE
                     "
     gene            12100..12402
                     /locus_tag="PLQ70_01035"
     CDS             12100..12402
                     /locus_tag="PLQ70_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQE33229.1"
                     /translation="METAVIIEIKDNTFARQFETITEKGLVSVEYSFQEKKIFLTKLN
                     VPDQFDDEEFISNLLKEIMAIAIERKLKVVPILPKIVVFFKKNPVYKELLPPGIRL"
     gene            12559..13008
                     /locus_tag="PLQ70_01040"
     CDS             12559..13008
                     /locus_tag="PLQ70_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018674988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQE33230.1"
                     /translation="METIFTPENLYSILSGRPHSLFNRALLSNFRKNNIALTKEQWTI
                     LGVLWQNDGCSQQNLADKTYRDKPSITRLIDNLEKENLVERRPDAKDRRLNLIFLTPK
                     GTQIEKKVIEIANQTLTDAIKGIDPQNLKIIKATFLQIYDNLEPKIK"
     gene            13005..14396
                     /locus_tag="PLQ70_01045"
     CDS             13005..14396
                     /locus_tag="PLQ70_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE33231.1"
                     /translation="MKKYLALTLISIFSFSNSNAQITISTSLDEAINKAIEKSSSIKN
                     KELEIEKINLQKKGVWNKYIPTVEASGLYTYFDNKLTIDAPSVTVPVVNLTLLDGKQT
                     FDNYGNLFNGSVMAKTILFSGMQIPNGAKALEQKAKGAEYLKESDKDDIIKEVINTFD
                     QLTLLNEVEKLINDSDKRLQSESKRVSKAIEQGLAVPYDRDKIKLASLELNSKKIELD
                     GKRKLVYRKIRYLTDYSETEINNVKHDLVPYILTSDKLTPENKQEIKALESFKIANEY
                     LLKKEKGTYLPTLGAFGGVTYSSLFDANTTTPVIPGLNKPLNLAINEMTLSPNWMFGA
                     AMKWEIFAGFEREHKIHEAKINIQQVQNQIDDTKEKLELLLENNYVNYSVLNQKIEIA
                     SQQEKVANNNLTLSIKQYKEGLINISERLEAENDSYKATVNKINTLIEQRLSAIETII
                     ATGELTQHISKLN"
     gene            14403..15356
                     /locus_tag="PLQ70_01050"
     CDS             14403..15356
                     /locus_tag="PLQ70_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQE33232.1"
                     /translation="MKKLIIVLAITSLFISCEKKINKGQIQGKIENEQIAVVSKIPGK
                     ILKIFVKEGDLVKKGDTLAILDIPEVTAKKNQAEGAVTSAKAQYNMAVKGATDNQIKQ
                     LEAKKQGLKEQYQFAQKSIKRLSNMLKDSLVSQQTYDEAFAKYEGAQAQYNAVVAELD
                     DAKKGGRIEQQTMALGQQDRAYGALQEVETADKERFVIAPQDMSIETITLNIGELALP
                     GYTLFNGYISNSTYFRFTIPESQLDKIKKGQEMTVHIFYNKKDIKGRVSTIKQLGAYG
                     NIATAYPDFEMQESLFEIKITPLNNQDAKDLITKATVTLTL"
     gene            15360..16517
                     /locus_tag="PLQ70_01055"
     CDS             15360..16517
                     /locus_tag="PLQ70_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE33233.1"
                     /translation="MRHFSTLLNREFKMFWQNKVLRLLFIGAPLLYGILLGYVYGKGK
                     VTDLPIIVVDEDRSEMSSKAIQMFEDNEVLNIADLLYDQKNLSQIAIEKEATCVVIIP
                     KGFEKMVLTKKYPEVVTIVNASNVLTANYASSALQLCLGTLKVGVQMETLRKQGTPEN
                     LIASQYEPFKTTFIKKYNRSTNYMYFLWPGVLAAVLQQVLLLGLALSFASEFEHGTFK
                     KLVQKCPSLFKLICVKIIPYLIMSFGLWIMYWLFTLWFRIPLYDNLFPLTIIAGIFIL
                     VVSFMGIMVSIMVPSQLKATEILMVVATPSFILSGFTWPLSQMPVWVQGIANVIPLTH
                     FLKAFRILIIEDGTFSQTYGVIINLLIIGSICAILSYIALYFKRKNVLKEI"
     gene            complement(16584..18218)
                     /locus_tag="PLQ70_01060"
     CDS             complement(16584..18218)
                     /locus_tag="PLQ70_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQE33234.1"
                     /translation="MITVNDISVQFGGTTLFSDVSFAINENDKIALMGKNGAGKSTLL
                     KIIAGQSKPSTGNISAPKEAVIAYLPQHLLTTDGATVMEEASKAFGEIFAMKAEIDEI
                     NEQLTVRTDYESDAYMKLIERVSDLSEKFYAIEEVNYEAEVEKILTGLGFERDDFSRQ
                     TSEFSGGWRMRIELAKILLRKPDLILLDEPTNHMDIESIQWLEDFLINAAKAVVVISH
                     DRAFVDNITNRTIEVTMGRIYDYKAKYSHYLELRKDRRVHQQKAYDEQQKFIADNQAF
                     IDRFRGTFSKTDAVQSRVKMLEKLEIVEVDEVDTSALKLKFPPAARSGQYPVIVKEMS
                     KSYGDHVVFKDANIVIERGQKVAFVGKNGEGKSTMIKAIMKEIGVDSGSVEIGHNSQI
                     GYFAQNQAALLDENATIFETIDAIAVGDVRTQIKNILGAFMFHGDDITKKVKVLSGGE
                     KTRLAMIKLLLEPVNLLILDEPSNHLDMKTKDIIKDALRDFDGTLILVSHDRDFLDGL
                     ATKVFEFGNKRVKEHFEDIAGFLAHKKMEHLREIEK"
     gene            complement(18348..19154)
                     /locus_tag="PLQ70_01065"
     CDS             complement(18348..19154)
                     /locus_tag="PLQ70_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQE33235.1"
                     /translation="MQTVRFIILSLLTLFGGIYLLYVGYVYCNQGELIFVANKLPNDY
                     KFEFKQDFEELNISSFDNKKLNGLLFKTPNPKGLVFYLHGNAGSLDTWGSIAENYTDL
                     GYDIFILDYRGFGKSEGEIESQDQVFKDLTFAYNKLITKYDRNKVVIIGYSIGTGLAT
                     YLASVEKPEKLILQAPYYNFIEFSSGRVPFVPDFLKKFKFETDKYIVKVKSPIYIFHG
                     NKDQVISCENSIRLQKLLKPTDKVFILDNQDHLGINENSDFQDELKRILE"
     gene            complement(19161..19964)
                     /locus_tag="PLQ70_01070"
     CDS             complement(19161..19964)
                     /locus_tag="PLQ70_01070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HQE33236.1"
                     /translation="MKYKMLVVDMDDTLLTDDHEISNENKEMLLKAQELGVYVILASG
                     RPTSAMIEYAKELQCDVNNSYMISYNGSTITDLKEDKILFEHSLTKEQIHSIYDFSQE
                     NNTHIITYLDGKIISETSSEYIDVESTITGLEHIIVPSFKDFVTTSAVKCLLLEEPSY
                     LKTVEPILKNAMPDLSVCMSKPFFLEAAPNGVDKGAAIEILAKKLNIHQSEIIAIGNA
                     GNDLTMVQYAGLGVWVDNVDDELREFGDVIVASNNDHGVAEVVRRFVLG"
     gene            complement(20020..20355)
                     /locus_tag="PLQ70_01075"
     CDS             complement(20020..20355)
                     /locus_tag="PLQ70_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQE33237.1"
                     /translation="MGCHKEGLVMEYKLKLLPIVYQDLRKAKKWYLEINTGLGEDFKL
                     KVNEELDYIKQYPKHYQKRYKELRQAIVGRFPYTIYYLIEEELKIVVVVGVLAQKQSF
                     DRIKKRINK"
     gene            complement(20328..20543)
                     /locus_tag="PLQ70_01080"
     CDS             complement(20328..20543)
                     /locus_tag="PLQ70_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HQE33238.1"
                     /translation="MDLSARKYSFIEEIFKVEEATFEKLEKVLKKEKLNKLGVPSEHK
                     DELDKRLEAYRENPKDLLDWDAIKKDW"
     gene            complement(20650..20868)
                     /locus_tag="PLQ70_01085"
     CDS             complement(20650..20868)
                     /locus_tag="PLQ70_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HQE33239.1"
                     /translation="MGLMKVFSGSEILALALQEKIEEAGVDTVLKNNIQSAKMAGFGT
                     SGQAVELFIQETDFAKANPVIEEFRMSI"
     gene            complement(20921..21583)
                     /locus_tag="PLQ70_01090"
     CDS             complement(20921..21583)
                     /locus_tag="PLQ70_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQE33240.1"
                     /translation="MKLKKINKILQDNLIANGLTEANEMQSETFSTLKSGADCIVIAP
                     KGSGKTTTIVINVIQKLVGQIEESPRALVFVETKEKVLEMVDLFNKYAKGTNLEIYGI
                     HDKGDTDYDKNYISTGVDILVGTPKKLSDMFSGAGYNVNRLKMFIVDDADAILKLRHE
                     TKIMRISDGIAKTQRIVFTDALTERIEILADKMLIEPFEFDFEEYDDEDFEDEEIDSE
                     EE"
     gene            complement(21699..22862)
                     /locus_tag="PLQ70_01095"
     CDS             complement(21699..22862)
                     /locus_tag="PLQ70_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQE33241.1"
                     /translation="MSKILIIEDEAAIRRVLTKILSEESDTYSVEDAEDGIQGLEKIK
                     NNDYDLVLCDIKMPKMDGVEVLEAVKKIKPEIPMVMISGHGDMETAIQTMRLGAFDYI
                     SKPPDLNRLLNTVRNALDRKQLVVENKILKKKVSKNYEMIGESEPINRIKLMIEKVAE
                     TEARVLVTGPNGTGKELVAHQLHEKSQRASFPLIEVNCAAIPSELIESELFGHVKGAF
                     TSAVKDRAGKFEAADKGTIFLDEIGDMSLSAQAKVLRALQENMITRVGADKDIKVDVR
                     VIAATNKDLKKEIAEGRFREDLYHRLAVILISVPSLNDRRDDIPLLISHFTEKIASEQ
                     GNAVKHFSAAAVKLLQEYDWTGNIRELRNVVERLIILGGNEISETDVQLFASK"
     gene            complement(22977..>23936)
                     /locus_tag="PLQ70_01100"
     CDS             complement(22977..>23936)
                     /locus_tag="PLQ70_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE33242.1"
                     /translation="SNESPSLSFIENVQDIIGRKLTKENFNKAHLDTLAIKNAESKVV
                     VNLAKASGEKSVKGLNEIKIAIGGAFGYLIMMFIIIYGNMVMRSVIEEKTNRIVEIII
                     SSVKPFQLMMGKIIGTSLAGLLQFFIWAILGFVLMIVASTFLGIDASPTAKIPPSVIK
                     EVHQEFGGMAQMYVRELWGLPIFTILFSFVIYFIGGYFLYSSFYASIGAAVDSETDSQ
                     QFLLPIIMPLVLAVYIGFFTVINDPHGTIATVFSMIPLTSPIVMLMRIPFGVPWWQIA
                     ISVTILFGTFFFVVWFAAKIYRVGILMYGKKTTWKEMYKWLKY"
BASE COUNT         7938 a         4498 c         3763 g         7637 t
ORIGIN      
        1 tatctatatt gaaaaacaag ataggaatgg taatcaacat taatatccaa taaatcaatt
       61 ttacggtgcg gtctgtcaag gaaactttgg gacaaaaata atgaaacgca aaatggataa
      121 atagacttgc gtaaggaata caaataaaaa acaaccattc ttcgatgggt aaatcaaaaa
      181 attcgattcc tgaatgataa cgaggattga aactccaaat gcctatttta gtaaaaaaaa
      241 catcccaaat aatgaagaaa gtagctgtga ttaaaatggc cgaaaaaaca gctttccacc
      301 gtttgatata gcgcatttta ttctcaaaac tgtatagaaa cggaattata aaggaagcta
      361 tatttaggaa taagtataat gacatttgtt ttttcttgta gatttaatct ttgtcaaagt
      421 ttaaaacttt gacaaagatt tcgaacgtat taaattttga aatatttaaa tggaacgaac
      481 aacatcccaa aacattcgcc ttcttctttt cctaaatgtt tgtggtgtac tttatgacct
      541 tttctaaggg caattaaata tttatttttg gtgtttttaa acaacttcaa acgttgatga
      601 ataattatgt cgtggacaat aaaatagctc aatccataag caaaaattcc caagccaatg
      661 aagaatcggt aatcaaaacc ggcatcagct ccgaagtaaa acaaaagaat actcggaatg
      721 gcaaaaatca caaaaaacaa atcattacat tcgaaaatat gggcatattt gggttggtgg
      781 tgatcttcgt gcaaatacca taaaaaaccg tgcattacat atttgtgtgt gagccaagtc
      841 acacattcca tcaacaaata agtggtcaaa aaaataaaaa aagacatcat tttcgaaagt
      901 tttaaaaggt aaaaatagtt aaaccaaatt caatttgtaa gatacgaaag aattagcaaa
      961 caacagccct ttggtgtaat tggaaactcg aattctttcg gttccaattt tgtggcttgg
     1021 tgttttttct agctttcgca acaatttttt atagtaaaca taagcagtat aaaccccgaa
     1081 tttggcttcc aaaggcaatt ttttgattcc ttgtaaagcc actcgaaaat cttcttcaat
     1141 ttcgttgaca atcataattt ttgatttttc atcaaaagaa tttaaatcga ctcccggaaa
     1201 ataatttcga tttaaaacca aattatcatc ttttaaatcc cgcaagaaat tcactttttg
     1261 gaaagccgaa cccaaacgca tcgcttcgtc tttgagttct tcatattttt ggttatcgcc
     1321 tttcacgaaa accttcaaac acatcaaacc cactacatca gccgagccat aaatgtattc
     1381 gtcatattct tgcttggtgt ggtaatctga tttgaccaaa tccattttca tacttttcag
     1441 aaacgattgc accaaatcat catgaatgtt gtactttttt accgtttgct gaaacgagtt
     1501 gagaatagga ttcaaactaa tgcccatttt ctgagtttta taatattcga tttcaaagtc
     1561 gttaatcaaa gtttctttat cgtaaccgtg aaaagaatcg acaatttcgt cagcaaaacg
     1621 aacgaaacca taaatactat aaatcgcttc tcgaatactt ggcgccaaca ttttaacagc
     1681 caaagaaaat gaggtgctgt aacttttggt caccaacacg ctacatttaa acgatacttc
     1741 gtcaaataaa ttcttcatat ttttgggtat taaaattgtt ttttaattaa atcagatact
     1801 atttttccag aaataataga tggtggaact cctggtcctg gaacagttaa ttgtcctgtg
     1861 aaaaataaat ttttgacttt tttactaatg attttaggtc ttaaaaaagc ggtttgggtt
     1921 agaatattcg ccaatccata agcatttcct ttgtatgaat tgtaatcttt gacaaaatct
     1981 ttgacacaaa aactttcttt aaagataatg ttttctgaaa tagtttgatt agtcaattgc
     2041 tcaaaacgtt cgataatgct tttgaaatag tgttctctaa tttcgggagt atcttcaatt
     2101 ccgggcgcaa ttggaatcaa aaatattccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnt aatcagctcc gcttaaaacc acatctgatt cgattgtgat tccgttagaa
     2281 accacgcttt tcaccactcc attttcaaca ttaatttttt caacattttg gttggtttca
     2341 atttttacac ccaattctac tgccaaagta accattgctt tgactacttc atacattccg
     2401 cctttgggat gccaagtacc caaaccaaaa tcggcataat tcataaaact ataaaaagaa
     2461 ggcgtattgg atggtttggc cccgaggaac aaaactggaa attccattat ttgctgtagg
     2521 cgattgtttt tgattttttt tcgaaccgaa gtacgaatcg tgctaaaaaa ctgattcact
     2581 tttttgatgg taacaggcgt aatcaattcg gttatggaaa ttccaggacg atagaccaaa
     2641 tctttgatgg ctacatcata attgtgttta gcattatcca aaaatgattc caaatgcttg
     2701 gcacttcctt tttcttcctt ttcgaaaata ttgaaaatat caggtaaatt atctggaatt
     2761 tgcaccgaat cggctttacc aaaatagact tcgtaagccg gattgagttt atctaaatgg
     2821 taataatccg aggggatttt accaaaatca gcaaaaaatt tctcgaacac atcgggcatc
     2881 caataccaag tgggaccaat atcaaaagta aaaccttgtt ttgaaagttg acgagctctg
     2941 ccaccaactg tgttattttt ttcaagaata gtaacatcaa aaccatcctt ggccaaatag
     3001 caagaagccg ctaaagccga aaatcctgaa ccgattattg taattgtttt tctcatttgt
     3061 tcaacaaaag taataaaaga ttaaacaaaa acaaatcttc ttaacaataa attacaattc
     3121 cttaaccaag tcaggaatgg aattaaagac agaaaccgct tttggaagtt tactaagatc
     3181 aatacactcg gtcattctgc ctaataacca aacttgagaa tcggcagtca ttatttctgt
     3241 agcaatttct tgtaaatatg tattcacttc atctttggtg ggctgaacgg tcagataagc
     3301 aacataaaca atgttgtcat aatactgctt catatctttt aaactttcga tgggaacgct
     3361 ttcacccaaa taaatggttt tatagccatt gagcaaaatt tcataattaa gatacatcaa
     3421 acccaattcg tgaatttcgt tttctggaag gtataaaaca aaaactttat cgtttcgagt
     3481 aggctctaaa acttgaattt tctcgatatt gaccaacaat ttttgcttga ttaaatagga
     3541 tataaaatgc tcttgcgcag gtgttatggt acctgattgc cacaacaacc caatttcgtt
     3601 catcaaagga ataaagactt cataaaaaac atcacggaac gatttttcgg aaagcaaatt
     3661 ggaataggtt ttgaaaaaca acacttgatc aaaattcatc attgccattt taaaagcatt
     3721 gatagcgtgg tttttggtgc ttttttctga aattatttct cgtaccaaca gttctatttt
     3781 ctcagaggaa tgttctgcaa ttcgagaaat tttatacccg taattgtgca acaaaacaat
     3841 gttgaggagt ttttgtagat tatgggtatc gtaatttctt atattggtat tggaacgcat
     3901 cggttcgaga atgttgtatc ttttttccca aattcggatg gtgtgtgctt tgattcccga
     3961 aaggttttcg aggtctttga tgctgaaaac agattttata ttgtttatca ttttttacaa
     4021 attaataaac aaatataatg aaatattttt taatctttac aaaacaactt cttcataatg
     4081 atttaattta tcgagataca aaaccgtttt ggtattagaa ctatttcgag aatacatcgg
     4141 caggaacttg gctccataaa ccacttcgtc aaaagtataa gaagttcgga tggcaaccgt
     4201 attactgaac atatcatcaa aagttttgat gaataccaaa atttcgccgt tgatggtttt
     4261 aaaatcttct ttggtaaaat gatacaaagg actatcctct gtaatgggat gcactaaagt
     4321 ccaactcaaa gttagcgcat tgattttatc caattcaaga tccaaggagt agaatttatt
     4381 gctcacaata cccgcttctt ctacactcat tcctaacatt attttagctt cggcatcggt
     4441 aaaattggtg tttttaaaag gcgttaatcg aagcatcaat gctgttccat cacgataagg
     4501 agcaataata gcgttgtgag aaaattttat aaacgcaact ggtttgctga agcgaccgaa
     4561 gaataaaccc gtagcaatgg caaaactcaa caaaccaatc aaggcttcta aagccgaaac
     4621 actactggct aaaaacccag aaggactcac gtggccataa cctactgtag taaaagtttg
     4681 tgcactaaaa aaataggtct ctccaaactt gacccattct gatgctgaag tatcaattcc
     4741 atttagatat tcgataccaa ttgcaaaata aattaaagca aaaacaagat tgataccaac
     4801 ataaaaagac aaaagaatgg acataaattt ccaagcaggc atctcaatca tcgtgtggta
     4861 ccaactgatg cgatgcagga aatgcattcc ctgtttttcg acattggcct tcccgttttt
     4921 attgaaaaaa cgtcctccgt aactacttgt gttgacccca aatcccgaac ttaaatagga
     4981 ttttgccttt tcgtttattc gttttaataa tgccattgtg gagttgtttc gtgcgaggta
     5041 aaattagtta aaaaaaatgt tctgatacaa cttaccaatc aaaggctttc aaaaatgcag
     5101 aaactgaatt gcaaaaaaaa tgaggtaatt cgttttttta ctaacttggt tgctccaaaa
     5161 actttcatta tgaaaaaact aaccaaagaa gatatcagtc aagaagtatt tgacctatac
     5221 gatgattacg cacacaataa aatcgaaaga agacaattca tcgaaaaact gtccattttt
     5281 gcagtaggaa gccttacact gccgtcgttg ctaagtttta tgacacccaa ttacgtggac
     5341 accatcacca ttaaagcgga cgatccaagg ttgaaatcag aattcattac ctacgaatcg
     5401 cccaaaggcg gaagcatcaa aggacttctt tcacaaccca cggaaaccaa gaaaaaatta
     5461 ccgggaatta ttgtcgttca cgaaaaccgt gggctgaatc cttacatcga agatgtggga
     5521 cgaagagctg ctctggaagg attcatcacc ttggcaccgg atgccttgtc accattgggc
     5581 ggttatcccg gaaatgatga tgcaggaaga gaattgcaaa aaaaacgtac tcgcgaggaa
     5641 atgctggaag actttattgc ggcttacgaa tacctcaaat cgcataaaga ttgcaacggt
     5701 tctgttggcg tggttggatt ttgttttggt ggttggattt cgaatatgat ggcggtaaaa
     5761 atccctactt tatccgcggc tgttccttat tacggaggac aacccactgc tgaagatgct
     5821 gaaaaaataa ccacaccgct attactaaat tatgccggtt tagacaccag agtaaacgaa
     5881 ggctggcccg cttttgaagc cattctccaa aagaacaaag tggaacatac cgcttatttc
     5941 tatgagggca tcaatcacgg ttttcacaac aatacgactc cgcgatatga cgaaaaagca
     6001 gctactttat cgtggacaag aactattgat ttttttaagg agaaattgaa gaagaaatag
     6061 tttttaaaga aaatactgtg aaataaaaaa ataagttata tttgaattga taattttttt
     6121 tatataaaac agacaataaa ttaattaaaa caaagtctta attgaatgaa attaaacata
     6181 atactacttt atttattgtt ttcaaaccta tggctttatt ctcaaaaaaa accaattgag
     6241 aacaatagta aagagtttct agctatagat tttaatggtt gcgtaaaaag cattgcaata
     6301 aaaagttatt cgtacaataa aaagaaagaa accattgata ctactgcttc atactataaa
     6361 atattctttg caaaaaatgg aaatattata aaacacatat attttgacaa atcatttact
     6421 attgaaaaga aaattatcga atatgatgaa ttagaaagaa ttaaaactat caaaatcaat
     6481 gatgatggaa aaatatggat agcttcagag caatttttca ataatttctc caattaccct
     6541 gattccacta atatttatta ttacggaaac aagtataaag aaaaatacat taacctgttc
     6601 gataaaaaaa acctcatcaa acaagaattt tactctcaag acaccttaag acattacaat
     6661 acttatattt acgataaaaa aaacagatta ataaaagatt attttactaa cacacaaaat
     6721 ggttttggca taactttagg aaaaagtata acaggagata aggatgaaaa aacactatgg
     6781 ccaaacgatt ccacaattta tgactacaaa aaaataaaag acactttaat aactataaaa
     6841 cttgaacaaa aaggttggaa agagattaaa aaagagttaa aatccaatat tttcacatta
     6901 gaaatattag aaaaatataa tcgtaatttt ttagagaatt ctagatatac atacaaatca
     6961 aaagattcga tttttgattg cacttattat tataaggaaa aaagggaggt taaaagtttt
     7021 tacaaaacaa ttacaacacc aaaaagcata gtttccaatt ggaaatctga tggattttat
     7081 aacaatgaag caagaacgga aacaacttat atcgagattg aatttgatgc ctacaataat
     7141 tggatagaga agaaatattc taaagacaat gccactattt ctctaattac aagagaaata
     7201 gaatattatt atcattgaca ttttatgaaa ccacaaaaaa agccccaaac ggggcttttt
     7261 caatatataa actcgagctt atttactcaa atacattttt cttcttgagt acaattcata
     7321 gaattggtcg tctttcaaat cgtcaataaa caagatactt tctcctgttg atttcatttc
     7381 tggtcctaat gctttgttta cattttggaa cttgctgaaa gagaaaaccg gttgtttgat
     7441 ggcgtatccg tttaattttg gattgaaatc aaaatcagtt actttctttt ctcctaacat
     7501 cactttggta gcgtagttca cataaggctc tccgtaagct tttgcaataa acggcaccgt
     7561 acgagaggct ctaggattgg cttcgatgat ataaacggta tcgtctttta tcgcaaactg
     7621 aatgttgatt aaccctactg ttttcaatgc ttttgcaatt ttaaaagtgt ggtctttgat
     7681 ttgttgcatt acaaattcac ccaagttgaa tggcggcaaa gtcgcattac tatcgcccga
     7741 gtgtacgcca caaggctcga tatgctccat aattccgatg atatatacat ttccatcagc
     7801 atcgcaaata gcatccgctt cggcttcgat ggctccagcc aaataatggt ctaatagcaa
     7861 tttattgcct ggaatcgatt tcaataaatt gataacgtgt tcttctaatt cttttttgtt
     7921 gattacaatt ttcattcctt gacctcctaa tacataagaa ggacgaatta ataaaggaaa
     7981 atctaaagta tccgccaaag cagaagcttc gtcagctgtt tcggcaattc cgaatttcgg
     8041 gaaaggaata ttcaattcag ttaacaaatc agagaaacgt cctctgtctt cggctaaatc
     8101 caaagcatca aagctagttc caatgatttt tactccgtat ttagacaatt tttcggccaa
     8161 tttcaaagca gtttgcccac ccaattgcac gataacccct tctggttttt cgtgttgaat
     8221 gatgtcgtaa atatgttccc aaaatacagg ttcgaaatac aatttatccg ccgtgtcaaa
     8281 atcagttgaa actgtttctg gattacagtt gatcataatg gtttcatagc cacattcttt
     8341 ggctgccaaa actccgtgaa cacaagaata atcaaactcg attccttgtc caattctatt
     8401 tggtcctgaa cccaaaacca ctattttctt tttatcggtt acgatacttt cgttgtgaac
     8461 gtaacgctct ccgtttgctt tttcgatttc agcttcgaaa gtcgagtaat agtaaggtgt
     8521 ttttgctgta aactctgccg cacaagtatc tactaattta aacacacgat tgatgttcat
     8581 agtcatacgc aaagtatgca cttcactttc tttgcaattc atcatatgcg caatttgtct
     8641 gtcggcaaaa cctttttgtt tggcttccaa caacaattct tttggcaaat tagcaacagt
     8701 ataagtagaa atttctttct ctaaaatata aagttgttcg tattgtttca agaaccacat
     8761 atcgatttta gtgatttcgt gaatacggct caacggaatt cccattgcaa tagcatcata
     8821 aatcacgaaa acacgatccc aactcgcaaa agtcaatttc tcgataatct gctcgtaatt
     8881 ggtatatcct tttccgtcag cacccaaacc atttcttttg atttccaatg attgagtagc
     8941 tttgtgcaag gcttcttgga acgaacgtcc aattcccatt acttccccaa cggatttcat
     9001 ttgaagtccc aaagtcctgt cggctccttc aaatttgtcg aagttccaac gtggtatttt
     9061 tacaattaca taatccaaag tcggctcgaa taaagcagaa gtagattttg taatttggtt
     9121 ttgcaattca tctaaattgt atcctaaagc tagtttagaa gcaatttttg caattggata
     9181 acctgtagct tttgatgcca aggctgagga acgagacaca cgaggattga tttcaatcgc
     9241 cacgatatct tctttttcgt ctggcgaaac ggcgaattgc acgttacaac ccccagcaaa
     9301 atttccgata ctgcgcatca tcaaaatggc catatctctc aatttttgga aagtcgtgtc
     9361 agacaaagtc attgctggtg caacagtaat cgaatctccg gtatgaattc ccattggatc
     9421 catattttcg atcgaacaga taatcacaac gttgtcgttt ttatctctca atagctctaa
     9481 ttcgtattct ttccaaccaa tcaaagcttt gtcaatcaac acttcgtgga tgggtgacat
     9541 ttccaatccg taagtcagtt tttcgtcaaa ttcttcttta gtatgtacaa aagccgctcc
     9601 agttcctcct aatgtaaatg aaggacgaat tactaacggg aaaccaaatt cctgtgcaat
     9661 ttcttttcct tccaagaaag aagtagctgt ttttgctggt gcggtaggaa catttatttt
     9721 ttgaagcaat tgtttaaact gctctctatc ttccgtaatg ttaatggcat ttacgtcaac
     9781 acctatcatt ttcactccga aatcttgcca aattcctttt tcatctgctt ccaaacacaa
     9841 gttcaaagcc gtttgtccac ccattgttgg taaaaccgca tcaatctgtg gatgtgcttt
     9901 taggatttca ataatggatt tggtagtcaa aggtttcaaa taaatatgat cggccataga
     9961 tgggtcggtc ataattgttg caggattcga gttaatcaag atcacctcaa ttccttcttc
    10021 acgaatagaa cgtgccgatt gggaacctgc ataatcaaat tcgcaagctt gcccaattac
    10081 gattggacct gaacctataa ttaaaaccga ttttatggag ttgtcttttg gcattgtgtt
    10141 gtttttggtg tttattttta cattaaagta taggatttta agttagttga accactttaa
    10201 atcttgaact tcaaacattt ttaatgacaa ggtataaaaa aaggcgatac taataacagt
    10261 aacgccttta tgtatgttta taaaaacatt attttttatg tctaggttcg ctagatacag
    10321 tcaatttgtg tcttcctttg gctctacgac gagctaggac ttttcttcca ttagcagaag
    10381 ccattctgtc cataaatccg tgcttatttc ttctttttct tttcgatggt tgaaacgttc
    10441 tcttgctcat tgctttgtat ctttaaaaaa tgtatttatg tgtctttggt agttttgaat
    10501 tccgttattc taaaaccgag tgcaaatata caaagacttt tatttctggc aagtggtttt
    10561 ataatttttt ttttaattcc ttttgttatc tttgctgata taaatttaca cattatgttt
    10621 cacaaaaata tcaaacttat tattactgta cttcttgtag ttacagccat ttggcaattt
    10681 acagaatcga atattggaaa cggaatcttt cttattttat tgactgcttt tccaattttt
    10741 ctttacttca aaaacgaatt tatcttattg gccttcctaa aattaagaaa acaagatttc
    10801 gatggtgcca aaaaatggtt agactacatc aaaaatcccg aaggagcatt ggttagaaaa
    10861 caacaaggtt attttaatta tttgcacgga ataatgcttt cgcaaaccaa catcaatcaa
    10921 tcggagaaat atttcaagaa agccattgag ttaggtttgt ctatggatat ggatttggct
    10981 gttgccaaac taaatttggc tggaattgca atgtcaagaa gaagaaaact cgaagcaacc
    11041 aacttattga acgaagcaaa aaaattagac aaacaaaata tgctaaccga tcagataaaa
    11101 atgatgaaag agcaaatgaa gaaactataa cccctcaccc cagccctctg agttaaaaag
    11161 ataaaaaggc gattagaaca aactctaatc gctttttttt attccaatat tattcgaggc
    11221 aagttttcgt tcagccattt gtactttgtt aatatcataa tatgggttcc tccttttatg
    11281 acaatgcaat tcttgatatt ttttatcgga aaaacatcat cggcatcacc gtgaatatga
    11341 accacatttt cgtcagcgac ggttctttgc cacaaaatta tttgctctat agcccaatcg
    11401 agataatgct tgtcgcgaac gcaaagaaat ttttcataca atttaagtcg ttgatttatt
    11461 gtttttccaa aagaaaacgt cgacaaactt tcgatattgg ccactaaagt ggttggaatc
    11521 aatttatagg ctttagctgt tttggccatt ttcattcttt tagggaattc tatattcgtt
    11581 ttgacactcg aaataatgat tacttttctg gcagtaataa attttgccat ttcttgaacc
    11641 aagatgccac caaaagatac tccgattaaa actggacttg aatgcgtaat cttatccgca
    11701 attcgtttgg catattgcgg taatgtttca ttatccaaag gaatttccca ttccaataga
    11761 atcatttcaa aaacgtcatc aggaagaaca attcgttcaa agattgaaga acttgctgcc
    11821 aaaccgggca taaaataaac ggggatttta ttcatcattg tgttttcaag caatccggtt
    11881 tacagtttcg gatttttgta taaaattaat tgttttattt gaaaccacta ccaatatact
    11941 gccaataatc gtttgaaatt gaaaatcaaa tcaatacctt tgttagcctt atcgccatcc
    12001 caaaataaaa acaactgatt tctttcaact ttgaaagtaa aatcaatttt agaccaaaat
    12061 acttttggct taaataccaa taaaattaaa taaaatagta tggaaactgc agtcattatt
    12121 gaaatcaagg acaatacttt tgccagacaa tttgaaacca taaccgaaaa aggtttggtt
    12181 tcggtagaat attcttttca ggagaaaaaa atatttttga ccaaactcaa tgttccagat
    12241 caatttgatg acgaagaatt catttctaat ttactaaagg aaattatggc gattgccatc
    12301 gaaagaaaac tcaaagtagt tcccattctt cctaaaattg tggtcttttt caaaaagaat
    12361 cccgtttaca aagaattact tcctccggga attcgattat aaaaaagagg ctgtttctaa
    12421 tattgaaata gcctttttta attattccaa actaataaat ttagatctat ctatttccta
    12481 acagaataaa ttttaaaaaa aaacaatgtc ctttaaataa attaaactaa ctttatagtt
    12541 gttcaaacaa ctattaatat ggaaactatt tttacacccg aaaatctata tagcatcttg
    12601 tctggaagac ctcattcgct tttcaataga gccttattga gtaattttag aaaaaacaac
    12661 attgctttaa ctaaagaaca atggacaatt ttaggagtgc tttggcaaaa tgatggttgt
    12721 tcgcaacaaa acttagccga caaaacttat agagacaaac ccagtatcac tcgtttgatt
    12781 gataatttag aaaaagaaaa tttggtagaa cggcgacctg atgccaaaga caggcgatta
    12841 aatttaattt tcctcacgcc caaaggaact caaatcgaaa aaaaagtaat tgaaattgca
    12901 aatcaaacac ttacagacgc tataaaaggc attgaccctc aaaatctcaa aatcataaaa
    12961 gctactttct tgcaaattta tgacaactta gaacctaaaa taaaatgaaa aaatatttag
    13021 ctttaaccct catctccatt ttcagctttt cgaacagcaa tgctcagatc acaatttcca
    13081 cttccttaga cgaagcaata aacaaggcaa tagaaaagag cagttccatt aaaaacaaag
    13141 aactcgagat cgaaaaaatt aatcttcaaa aaaaaggagt ttggaataaa tacattccca
    13201 ctgtagaagc tagcggactt tacacctatt ttgacaataa attaacaatt gacgcccctt
    13261 cggtaaccgt tcctgtggtc aatttaactt tgcttgacgg aaagcaaact ttcgataact
    13321 atggaaatct cttcaatgga agcgtgatgg caaaaaccat tttgttcagc ggaatgcaaa
    13381 ttccgaatgg tgcaaaagca ttggaacaaa aagctaaagg tgccgaatat ttgaaagaat
    13441 ccgacaaaga tgacattata aaagaggtga ttaatacttt cgaccaatta actttgctca
    13501 acgaagtaga aaaactaatc aatgacagcg acaaaagatt gcaatcggaa tccaaaagag
    13561 tttcaaaagc tatcgaacaa ggcctcgccg ttccttatga tagagataaa ataaaattag
    13621 catcattaga attgaattcc aaaaaaatag agctagacgg aaaacgtaaa ttagtctatc
    13681 gaaaaattcg ctacttgact gattattccg aaacggagat taataatgta aaacacgact
    13741 tggttcctta tattctaact tcagacaaac taacaccaga aaataaacaa gaaatcaaag
    13801 ccttagaatc cttcaaaata gctaatgaat atttattaaa aaaagaaaaa ggaacttatt
    13861 tacctacttt gggagctttt ggaggcgtaa cttattcgag ccttttcgat gccaacacca
    13921 caactccagt gattccggga ttaaacaaac ctttgaattt agccatcaac gaaatgaccc
    13981 ttagccccaa ttggatgttt ggagccgcta tgaaatggga aatatttgcc ggtttcgaaa
    14041 gagaacataa aattcacgaa gccaaaataa atatccaaca agtgcaaaat caaattgacg
    14101 ataccaaaga aaaattggaa ttacttttag aaaacaacta cgtaaattat tcggttttaa
    14161 atcaaaaaat agaaattgca agccagcagg aaaaagtggc taacaataat cttaccttgt
    14221 ccataaaaca atataaggaa ggtttaataa acatttcgga acgccttgaa gccgaaaacg
    14281 attcctataa agcaaccgta aacaaaataa atactcttat agaacaacgc ttatccgcca
    14341 ttgaaactat tatagctaca ggcgaactca cccaacatat atcaaaatta aattaatagt
    14401 ttatgaaaaa attaatcatt gtactggcaa tcacttccct ttttattagt tgtgagaaaa
    14461 agataaacaa aggacaaatt cagggaaaaa tagaaaacga acaaattgct gtagtgagca
    14521 aaattccagg aaaaatttta aaaatatttg tcaaagaagg agatttagta aaaaaaggcg
    14581 atacacttgc catactggac attcctgaag ttactgccaa aaaaaaccaa gcggaaggag
    14641 ctgtaacttc tgcaaaagca caatacaata tggcggtaaa aggcgcaacc gacaaccaaa
    14701 taaaacagct tgaagccaaa aaacaaggac tgaaagaaca ataccaattt gcccaaaaat
    14761 ccatcaaaag attgagtaat atgctcaaag attctttggt ttcccaacaa acctacgacg
    14821 aagcttttgc caaatacgaa ggcgcacaag cacaatacaa tgccgtggta gccgaactag
    14881 acgatgccaa aaaagggggt cgaatcgaac aacaaactat ggctttagga caacaagaca
    14941 gagcttatgg cgcgttgcaa gaagtagaaa ctgccgataa agaacgcttc gtaattgcgc
    15001 cacaagatat gagtatcgaa actataacgc taaatattgg cgaattggca ttaccaggtt
    15061 acactttgtt caacggttat atttcaaata gcacgtattt ccgttttaca attcccgaaa
    15121 gtcagttgga taaaattaaa aaaggacagg aaatgaccgt tcacattttc tacaacaaga
    15181 aagacattaa aggaagggtt tccaccatca aacaattggg agcttacgga aatattgcca
    15241 ccgcctatcc tgattttgaa atgcaggaaa gcctattcga aataaaaatt acccctttaa
    15301 acaaccaaga tgcaaaggat ttaataacaa aagcaaccgt aactttaacg ctataaatta
    15361 tgcgacattt ctccacgctt ctcaaccggg aattcaaaat gttttggcaa aataaagttc
    15421 ttagattact ttttatagga gcccctttgc tgtatggaat tttattaggt tacgtttatg
    15481 gcaaaggaaa agtcaccgat ttgcccataa ttgtagtcga tgaagaccgt agcgaaatga
    15541 gttcgaaagc catccagatg tttgaagaca acgaagtact gaatatcgcc gatctcctgt
    15601 atgaccaaaa gaatctttcg caaattgcca tcgaaaaaga agctacttgt gttgtgatta
    15661 ttccaaaagg cttcgaaaaa atggtattga ccaaaaaata tcccgaagtc gtcacaatag
    15721 taaatgcatc gaatgtttta actgccaatt atgcctcttc tgcattacaa ctctgccttg
    15781 ggacattaaa agtaggcgtt caaatggaaa ccttacgcaa acaaggcaca cccgaaaacc
    15841 tgattgcttc acaatacgaa cccttcaaaa caacctttat caaaaagtac aaccgaagca
    15901 ctaactatat gtatttcctt tggcccggag ttttagcagc ggttttacaa caagtattac
    15961 tgttgggatt agcactttct tttgcctcag aatttgaaca cggaacattc aaaaaattag
    16021 ttcaaaaatg cccctcttta ttcaaattga tctgcgttaa aatcattccc tatctgataa
    16081 tgagttttgg actttggata atgtattggc tctttacact ttggtttaga attccattgt
    16141 acgacaacct tttccccctg acgattattg caggaatatt tattttagtg gttagtttta
    16201 tgggaattat ggtcagtatt atggttccat ctcaactaaa agcaaccgaa atactaatgg
    16261 ttgttgcaac gccaagcttc attttaagtg gttttacttg gccattaagc caaatgccag
    16321 tttgggttca gggaattgcc aatgtgattc cattaaccca tttcttgaaa gcttttcgaa
    16381 ttttgattat cgaagacgga accttttcgc aaacttacgg agtcatcatc aacttgctaa
    16441 ttattggttc catttgcgct attctctctt atattgcgtt gtatttcaaa agaaaaaacg
    16501 ttttaaaaga aatctaaatc caataaaaaa agtccgatac ttgatcatta agtatcggac
    16561 tttttaaata tattctaaaa aaactatttc tcgatttctc tcaaatgctc cattttcttg
    16621 tgtgccaaga aaccagcaat atcttcgaaa tgctctttca ctcttttatt tccaaattca
    16681 aaaactttgg ttgccaatcc atccaagaaa tccctgtcgt gcgaaaccag aattaaagtt
    16741 ccgtcaaaat cacgcaaagc atctttgata atatctttcg tcttcatatc taggtgattc
    16801 gaaggctcat ccagaattaa taaatttacg ggttctagca ataattttat cattgccaaa
    16861 cgggttttct caccacccga aagtactttt acttttttgg taatatcatc tccgtgaaac
    16921 ataaacgcac ccaaaatatt cttgatttga gttcgaacat cgcctaccgc aatagcatca
    16981 atggtttcaa aaatagtagc attttcatct aataaagcgg cttgattttg ggcaaaataa
    17041 ccaatttgtg aattatgtcc aatttccaca gaaccactat caacgcctat ttctttcata
    17101 atagctttaa tcatcgtcga ttttccttct ccatttttac caacgaaagc tactttttga
    17161 cctctttcaa tcacgatatt cgcatctttg aaaaccacgt gatcgccata agatttagac
    17221 atttctttga caatcactgg atattgtcct gaacgagctg ctggtggaaa tttcaatttc
    17281 aaagctgatg tatccacctc atctacttca acaatttcca atttttcaag cattttcacg
    17341 cgagactgaa cggcatcggt tttcgaaaaa gtacctcgaa atctatcaat aaatgcttga
    17401 ttgtctgcaa tgaatttttg ttgctcatca taagcttttt gctggtgaac acgacggtct
    17461 tttctgagtt ccaaataatg ggaatatttg gctttgtagt cataaattcg ccccatcgtc
    17521 acttcgatag tacgattggt aatattatcg acaaaagctc tgtcgtgaga aatcaccaca
    17581 acggctttgg cagcattaat caagaaatcc tccaaccatt gaatactttc gatatccata
    17641 tggtttgtag gctcatccag caaaatcaag tctggttttc gcaaaagaat tttggccaat
    17701 tcgattcgca ttctccaacc gcctgaaaat tcggaggttt gacgagagaa atcgtctcgt
    17761 tcgaaaccca aaccagtcaa gattttctcg acttcggctt cgtaattcac ttcttcgatg
    17821 gcataaaatt tctcgctcaa gtccgaaact ctttcgatta atttcatata agcatcgctt
    17881 tcataatccg tacgaacggt cagttgctcg ttgatttcat cgatttcggc tttcattgca
    17941 aagatttctc caaaagcttt agaagcttct tccataaccg ttgcgccatc ggtagtcaat
    18001 aaatgctgag gcaaataagc aatcacggct tcttttggtg ccgaaatatt tcctgttgag
    18061 ggtttgcttt gccctgcaat aatcttcaaa agcgttgatt ttcccgcacc atttttaccc
    18121 ataagggcaa ttttgtcatt ttcgttgata gcaaaagaaa catcactaaa taaagtggtt
    18181 ccgccaaatt gaacggagat atcgttaact gtaatcattt ttcgaagttt gaagttggga
    18241 gtttgaagtt tgaagattca aagtcctaaa aattaaaagt ggcaaagata gcttaaataa
    18301 ggaattgggc aatttttagg aatgtagaat taatttcaaa aaattaatta ctctaaaatc
    18361 cttttcaatt cgtcttgaaa atcagaattt tcatttatgc ctaaatggtc ttgattgtcc
    18421 aaaataaaaa ctttatcagt cggcttcaga agtttttgca atcgaatgga attttcacaa
    18481 gaaatcacct gatctttgtt tccgtgaaaa atataaatgg gggattttac ttttacaatg
    18541 tatttgtcgg tttcaaattt aaacttcttc aaaaaatctg gaacaaaagg aactctgccg
    18601 cttgaaaatt caataaaatt ataataagga gcttgtagaa ttagtttttc aggtttttca
    18661 actgatgcta aataggttgc caatccagtt ccgatggaat atccaattat cacgacttta
    18721 ttcctgtcat atttcgttat cagcttgtta tacgcaaaag ttaaatcctt gaaaacttgg
    18781 tcttggctct cgatttctcc ttcacttttc ccaaaacctc tataatccaa aataaaaata
    18841 tcgtatccta aatcggtata attctcggcg atacttcccc aagtgtcgag cgaacctgca
    18901 tttccgtgaa gataaaacac taatcctttt gggtttggcg ttttgaacaa tagtccattt
    18961 aattttttgt tatcaaatga agaaatattc aattcttcga aatcttgctt aaattcaaac
    19021 ttataatcat ttggcaattt atttgccaca aaaatcagtt ctccctgatt acaataaaca
    19081 taaccaacgt aaagcaaata aattcctccg aaaagagtta aaagcgatag aattataaac
    19141 cggacagttt gcataatttt ttaacccaaa acaaatctcc tcaccacttc ggcaacaccg
    19201 tgatcattat ttgaagctac gataacatcg ccaaattctc ttaattcatc atcgacattg
    19261 tcaacccaaa cacccaaccc ggcatattgc accatagtca aatcgtttcc ggcatttcct
    19321 atggcgataa tttcagattg atgaatgttt agttttttgg ccaaaatttc aatggcagct
    19381 cctttgtcaa cgccgttggg tgcggcttcc aagaaaaagg gtttcgacat acaaacactc
    19441 aaatcaggca tcgcattttt caatatgggc tccacagttt tgagataact gggttcttca
    19501 agcaacaaac attttaccgc agaggtggtc acaaaatctt taaaacttgg cacaattata
    19561 tgttccaaac ccgtaatggt actttcgaca tcaatatatt ctgaactcgt ttcactaatt
    19621 atttttccgt ctaaatacgt gatgatgtgt gtattatttt cttgactaaa atcgtaaatg
    19681 gaatgaattt gctctttggt caaagagtgt tcaaaaagaa ttttgtcttc ttttaaatca
    19741 gtgatagtag aaccgttata ggaaatcata taggagttat tgacatcgca ttgcaattct
    19801 ttggcatatt caatcatcgc cgatgtgggt ctgcctgaag ccaaaatcac ataaactccc
    19861 aattcctgtg ctttgagaag catttcttta ttttcgtttg atatttcgtg gtcgtctgtc
    19921 aacaaagtat cgtccatatc gaccactagc attttgtatt tcattgtttt tattttataa
    19981 aaaaatcatc aaaaaaccgc tcttttgaag cggtagttgc tatttgttta ttcgtttttt
    20041 aattctgtca aaactttgct tttgggctaa aactcccact acaacaacta ttttcaattc
    20101 ttcttcaatt aaataataaa tggtataagg aaatcgcccc actattgctt gtcttaattc
    20161 tttgtacctt ttttggtaat gctttggata ttgcttaata taatccaact cttcgtttac
    20221 tttcaactta aaatcttcgc ctaatcctgt atttatttct aaataccatt tttttgcttt
    20281 tcttaaatct tgataaacaa ttggcaataa tttcaacttg tattccatta ccaatccttc
    20341 tttatggcat cccaatccaa taaatctttt ggattttctc tataggcttc taatcgctta
    20401 tccaattcat ccttatgctc tgagggaact ccaagtttat tcaacttttc ttttttcaaa
    20461 accttttcca gtttctcaaa agtagcctct tctaccttga aaatctcctc aataaaacta
    20521 tattttctag ccgataaatc cataataccg ctttttatta tacaaatata cgaatttcca
    20581 ttaatacttt tgtttttata tatggaacaa gaattgaaca aataccactt aaacctttat
    20641 aattccgctt tatatactca tcctaaactc ctcaataaca ggattcgcct ttgcaaaatc
    20701 ggtttcttga atgaataatt caacggcttg accgcttgtc ccaaaacctg ccattttagc
    20761 cgattggatg ttgtttttca atacggtatc gactcccgct tcttctattt tttcttgcaa
    20821 agccaaagcc aaaatttcgc ttcccgaaaa cactttcatt aatcccatat tattgtttat
    20881 tgtttgttgt tgatagttta ttgttactca taaacagtaa ttattcttct tctgaatcga
    20941 tttcttcgtc ttcaaaatcc tcatcgtcat attcttcgaa atcaaattca aaaggttcta
    21001 ttaacatttt gtctgccaga atttctattc tttctgtgag agcatctgta aaaacaatgc
    21061 gttgtgtttt ggcaatacca tccgaaatac gcataatttt agtttcgtgg cgtaatttca
    21121 aaatagcatc ggcatcatca acgataaaca tttttagtct atttacgtta tatccagctc
    21181 cactaaacat atcactcaat tttttgggtg ttcctaccaa aatatctact cctgttgaaa
    21241 tgtagttttt atcataatca gtatctcctt tgtcgtgaat tccgtatatt tcaagattag
    21301 tccctttggc atatttgtta aacaaatcga ccatttcaag caccttttct ttagtttcta
    21361 caaataccaa agcgcgtggc gattcttcaa tttgaccaac caatttctga atgacattga
    21421 ttacgattgt cgtagttttt ccgcttcctt ttggagcaat aacaatacaa tcggcaccac
    21481 tttttagggt cgaaaaagtt tctgattgca tttcattggc ctcggttaaa ccattagcaa
    21541 ttagattgtc ttgcagaatt ttgtttattt tttttagttt cattttattg ttttttacac
    21601 aaagattctc aaagtaaaaa agaagattca cgaaggttta aattaaaaaa actttgtgct
    21661 tctttgcgcc aactttgtga atctttgtga aatagtattt attttgatgc gaacaattgt
    21721 acatccgttt ccgaaatttc gtttccgccc aatattatca agcgttccac tacatttcgc
    21781 aattctcgga tatttcctgt ccaatcgtat tcttgcaata atttgacagc tgctgctgaa
    21841 aaatgcttca ctgcatttcc ttgttcagaa gcgatttttt cggtgaaatg ggaaatcaat
    21901 aacggaatgt catctcgcct atcatttaac gatggaacac taattaaaat cactgccaat
    21961 cgatggtaca aatcctcacg gaaacgacct tcagcaattt cttttttcaa atctttattc
    22021 gttgcagcaa tgacacgaac atcgactttg atgtctttgt cggctcctac tctggtaatc
    22081 atattctctt gtaaagcgcg taatactttg gcttgagctg aaagactcat gtcgccaatt
    22141 tcatccaaaa aaatagttcc tttgtcggct gcttcaaatt tccctgctct atctttgact
    22201 gctgatgtaa aagcgccttt gacgtgacca aacaattcgc tttctattaa ttcgctagga
    22261 attgcggcac aattgacttc aattaaagga aaactggctc tttggctttt ctcgtgcaat
    22321 tgatgggcaa cgagttcttt tccagtccca tttggtccag taactaaaac tctagcttcg
    22381 gtttcagcta ctttttcaat catcaatttg atgcgattga tgggttcgct ttcgccaatc
    22441 atttcgtagt tcttgctgac ttttttcttc aggattttat tctcaaccac gagttgtttt
    22501 ctgtctaaag catttcgaac cgtgttcaat aatcggttta aatcgggcgg ttttgaaata
    22561 taatcaaaag cgcccaaacg catcgtttga atagccgttt ccatatcgcc gtgaccagaa
    22621 atcatcacca tcggaatttc gggttttatt tttttaaccg cttccaaaac ctcaacgcca
    22681 tccattttgg gcattttgat gtcgcacaaa acgagatcgt agtcgttgtt ttttattttt
    22741 tccaagcctt ggattccatc ttcagcatct tctacactat aagtatcgct ttcttccgaa
    22801 agtattttgg ttaatactct tcggatagcg gcttcgtctt ctatgattag tattttactc
    22861 atttttttaa ggttctgaga ttcttagatg ctaaggttct aagtttagtg ttttcttaaa
    22921 tcatccgaaa ctaaaaactt agtctctcag aatcttagca acttaggtac tctaaattaa
    22981 tatttcaacc atttatacat ttctttccaa gttgtttttt taccgtacat taaaattcct
    23041 actcggtata tttttgctgc aaaccaaacc acaaaaaaga atgttccaaa aaggattgtt
    23101 actgaaatag cgatttgcca ccaaggtacg ccaaacggaa ttcgcatcaa cattacgatg
    23161 ggcgaagtga gcggaatcat cgaaaataca gtagcgattg ttccgtgagg atcattgatt
    23221 acggtaaaaa agccaatata aactgctaga accaaaggca taataatggg caaaagaaat
    23281 tgttgagagt cggtttcgct atctaccgca gcgccaatag aagcgtaaaa ggaactgtac
    23341 aaaaagtaac ccccaatgaa ataaatcaca aaactaaaaa ggattgtaaa aataggcaag
    23401 ccccataatt cccgaacata catttgcgcc attcccccaa attcttggtg aacttctttt
    23461 attactgaag gaggtatttt tgctgttgga ctagcatcaa tacccaaaaa agtcgaagca
    23521 acaatcatta atacaaatcc aagaatggcc caaataaaga attgcagcaa tcctgccaat
    23581 gaagttccta ttatttttcc catcatcaat tgaaatggtt ttactgatga gataataatt
    23641 tcgacaatac gattggtttt ttcttctata acactgcgca tcaccatatt accataaata
    23701 ataataaaca tcataataag atatccgaat gcacctccaa tggctatttt aatttcatta
    23761 agtcctttta cacttttttc accagaagct ttggctaaat taaccactac tttggattca
    23821 gcatttttta tggctagagt gtctaaatgt gccttattga aattttcctt agtgagtttt
    23881 cttcctataa tatcttgaac attttctata aaagacaaac tgggactttc gttgga
//
LOCUS       DAOSNN010000010        23827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_2145_length_23827_cov_5.548418, whole genome shotgun sequence.
ACCESSION   DAOSNN010000010 DAOSNN010000000
VERSION     DAOSNN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2145_length_23827_cov_5.548418"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..859
                     /locus_tag="PLQ70_01105"
     CDS             164..859
                     /locus_tag="PLQ70_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQE33243.1"
                     /translation="MTFDSPADVTIKNNILIVDDHPFIIQGYKNAITRYNPKQYEFLI
                     TEAKDCESGYNVITNPETSPFDIAFLDISMPSYDEKGIFSGEDLAKLLNEYMPNCKII
                     LLTMYTELLKIRTIIDAINPLGLVIKNDLTFDELLFGFNKVINNETYYSQSIQKMLDQ
                     SEMEAIEIDLFDKQILFHISKGTKTKDIPQYVPISLSAIEKRKMNLKKILSITDETDI
                     ELVRVAKNRGLLF"
     gene            complement(913..1962)
                     /gene="thiL"
                     /locus_tag="PLQ70_01110"
     CDS             complement(913..1962)
                     /gene="thiL"
                     /locus_tag="PLQ70_01110"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HQE33244.1"
                     /translation="MIEDKNPQRTSIAQLGEFGLIEHLTKNFEINQTSTLKGIGDDAA
                     VLNFADKKVVVSTDILVEGVHFDLAYMPLKHLGYKAVVVNVSDICAMNAKATQILVSI
                     AVSNRFPLEALEELFDGISLAANEYKIDVIGGDTTSSQKGMIISITVLGEADENEIVY
                     RNGAGQTDLLVVTGDIGAAYMGLQVLEREKQVFQVNPNSQPDLDAYTYLIERQLKPEA
                     RKDIRTLLHALEIQPTAMIDISDGLSSEIMHICKQSKVGCNLYEDKLPLDPQFMNVCE
                     EFNLDATTVAINGGEDYELLFTIKMEDFEKIKGNPNFTIIGHMTQESEGIHLVTRANT
                     RIPLKARGWNALSEE"
     gene            complement(2131..2871)
                     /gene="mtgA"
                     /locus_tag="PLQ70_01115"
     CDS             complement(2131..2871)
                     /gene="mtgA"
                     /locus_tag="PLQ70_01115"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQE33245.1"
                     /translation="MATKITSKKTKKPAKKESGTFKSKVSRFLFKAMLWFFGVSIFLV
                     VLFKFVPVPFTPLMVIRTIENKLAGKENYYSHDWEPIENISLNLQKAVIASEDGTFLS
                     HHGFDFTAMQKAYKNNERGRRIKGGSTISQQTAKNVFLWQGRSYLRKGLEAYFTILIE
                     LIWGKERIMEVYLNSIEMGNGIYGAQAATQHWYRKDAKSLTKIQAAGIAAILPNPRKY
                     SATSSSSYINRRKDKIVRIMRHIGKINY"
     gene            3001..4902
                     /locus_tag="PLQ70_01120"
     CDS             3001..4902
                     /locus_tag="PLQ70_01120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQE33246.1"
                     /translation="MKKIAFIILIMMNLNAMAQNTMSPEMLLKLGRISALGLSKDGKN
                     IIYKVDTPSAEENKSNSKLYSLPINGGNPIEIASAKEVLNDKNISPDGKFIVYDQEVK
                     IDKVHGKDFYPELDKSNVQIYNGLDYRHWDKWNEGKFNHVFYKENKEGADGIDILKGE
                     NFDSPQKPFGGDEDYIWSPDSKSIIYVCKKKAGTAYAISTNTDLYEYNLETGKTTNKT
                     EGNLGYDTHPTFSPTGNLTWLQMKRDGYEADKNDIIVQFKGININLTAHWDGTVESFL
                     WSPDGKKVYFQAPIDGTIQLFEVDFPGLTKKLPQVVQLTNGDFDVSGLIALSGDSIFV
                     TRTDMNHAAEIYSYNLQQKTWHQLTNVNTETYNSLALSKTERRYVTTTDGKKMLVWVI
                     LPPHFDASKKYPTLLYCQGGPQSALTQFYSFRWNFQLMAAQGYIVVAPNRRGMQGHGV
                     EWNEQISKDWGGQVMQDYLSAIDDVAKENYVDKNRLGCVGASYGGYSAFYLAGIHNNR
                     FKTFIAHDGVFNTQSMFGTTEEVFFNYWDFGGAYWEKDNAVAQKAYTVFNPINNVQKW
                     NTPILIIQGGNDFRVPIGQGQEAFQAAQMLGIQSRFLYFPEENHWVLKPQNALVWQRE
                     FYKWLKETL"
     gene            5015..5863
                     /locus_tag="PLQ70_01125"
     CDS             5015..5863
                     /locus_tag="PLQ70_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HQE33247.1"
                     /translation="MKTSFIAFLLFCGICFGQNNTDTSLYMKSDKVTYLTDIGSESGD
                     LYKTIGHHGPAIENEWMALRIYFNEKVAIDVYSKPNLGLELRKANWYPTPEQQKEGWG
                     ADYYKVNATVGLGGVKLWDGEKVVPLNPVSNRLARVGKTDTTSWMEMISKGVSYKGQK
                     VDILVRVTVFSGKREAKVEAICLSKTKVQFVTGVNYFKDFKSDKGSNYIAVWGMHPED
                     VAASKVEVGAAIKYNPKDYLKNFDDGTQYLLVSKPTKHLETTIISSCAIEKDLNSFEK
                     LVAFLK"
     gene            complement(5982..7328)
                     /gene="accC"
                     /locus_tag="PLQ70_01130"
     CDS             complement(5982..7328)
                     /gene="accC"
                     /locus_tag="PLQ70_01130"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQE33248.1"
                     /translation="MFKKILIANRGEIALRIIRTCKEMGIKTVAVYSTADAESLHVKF
                     ADEAVCIGPPPSSLSYLKMSNIIAAAEITNADAIHPGYGFLSENSKFSKICQEHNVKF
                     IGAAPEMIDRMGDKASAKATMKAAGVPCVPGSDGLLESFEQAQKLSKEMGYPVMLKAT
                     AGGGGKGMRAVWKEEDLLKAWESARQESAAAFGNDGMYMEKLIEEPRHIEIQIVGDAY
                     GKACHLSERDCSVQRRHQKLTEETPSPFMTDELRQKMGEAAVKAAEFIQYEGAGTVEF
                     LVDKHRNFYFMEMNTRIQVEHPITEQVIDYDLIREQIMVAAGIPISGKNYLPELHAIE
                     CRINAEDPYNDFRPSPGKITTLHMPGGHGVRLDTHVYSGYTIPPNYDSMIAKLITTAQ
                     TREEAISKMRRALDEFVIEGIKTTIPFHRQLMDDPKYIEGDYTTAFMDTFKMNDPE"
     gene            complement(7422..7904)
                     /gene="accB"
                     /locus_tag="PLQ70_01135"
     CDS             complement(7422..7904)
                     /gene="accB"
                     /locus_tag="PLQ70_01135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HQE33249.1"
                     /translation="MDLKEIQNLIKFVANSGVAEVKLEMDDVKITIRTTLEGNVTEAT
                     YLQMPTAQPALQPAVVPQQIAPVATPAAPAAPAENSNYITIKSPIIGTFYRKPSPDKP
                     MFVEVGSTIAKGNVLCVIEAMKLFNEIESEVSGKIVKILVDDMSPVEYDQPLFLVDPS
                     "
     gene            complement(7928..8926)
                     /locus_tag="PLQ70_01140"
     CDS             complement(7928..8926)
                     /locus_tag="PLQ70_01140"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQE33250.1"
                     /translation="MNKITAAITAVGAYVPDYVLTNSILETLVDTNDEWITTRTGIKE
                     RRILKDDAKGTSYLAIKAAQDLINKSNIDPLEIDLVLVATATADMLVASTGAYVATEI
                     GATNAFSYDLQSACSSFLFGMSTAAAYIESGRYKKVLLIGADKMSSIVDYTDRATCII
                     FGDGAGAVLFEPNNEGLGLQDEYLRTDGEGREFLKMTAGGSLFPASFETVRDNQHNIM
                     QDGKTVFKYAVTNMADACETILKNNNLTNNDVQWLVPHQANKRIIDAIANRMNLEDSK
                     VLMNIERYGNTTSATLPLVLSDFEHLFKKGDTIIFAAFGGGFSWGSIYLKWAYDKK"
     gene            complement(9099..9293)
                     /gene="rpmF"
                     /locus_tag="PLQ70_01145"
     CDS             complement(9099..9293)
                     /gene="rpmF"
                     /locus_tag="PLQ70_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQE33251.1"
                     /translation="MAHPKRKTSKTRRDKRRTHYTATVAQIATCPITGEAHLYHRAYW
                     HEGKMYYRGQVVIDKSVAVA"
     gene            complement(9303..9854)
                     /locus_tag="PLQ70_01150"
     CDS             complement(9303..9854)
                     /locus_tag="PLQ70_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQE33252.1"
                     /translation="MDKKKEFLIPFIGLKLGKHHFEYQINKAFFEIFDYHEFNDSDIK
                     VNVVLEKKSTMLELAFKHKGTVNVPCDMTNEEFDLPIKGKMKLIVRFGDAFNNDNEEL
                     LIVPHGEFQIDISQYIYEMIVLSVPLRRIHPGVKDGSLNTEALAKLKELTIKELKKEH
                     TKEQKQEDTDPRWDKLKQLLTDK"
     gene            complement(9945..10988)
                     /gene="pdxA"
                     /locus_tag="PLQ70_01155"
     CDS             complement(9945..10988)
                     /gene="pdxA"
                     /locus_tag="PLQ70_01155"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HQE33253.1"
                     /translation="MIKKAENIIVGISIGDLNGIGSEVILKTFEDSRMLELCTPVIFA
                     NVKVVSFIKKTLESTSMLQGIDRLDQIVLGKINVLNVWQEAVEINFGVNDEKIGKYAI
                     KSFLAATKALKEGLVDVLVTAPINKYNIQSEDFKFPGHTDYLDQELEGNALMLMVQDN
                     LRVGLLTDHIPLNEVASHLTEELIVKKIETIKQTIIQDFSINKPKIAVLGLNPHCGDG
                     GVIGKEDDAVLKPALKKIFDKGTLVFGPFAADGFFGSNQYDKYDAIIATYHDQGLIPF
                     KTLSFGNGVNYTAGLNKIRTSPDHGTAYDIAGKGIADYNSFKEAVYLAIDVYNSRNQY
                     AEISQKPLKTKEK"
     gene            11046..11633
                     /locus_tag="PLQ70_01160"
     CDS             11046..11633
                     /locus_tag="PLQ70_01160"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQE33254.1"
                     /translation="MFTGIIESLGVIQEIKNDKDNVHITIDSSITNELKIDQSVAHNG
                     ICMTVVSIDNSSYTVTAIAETIKKTNLSHLKVGDSVNLERAMKLGDRLDGHIVQGHVD
                     QVGTCKVANETNGSWYYTFEYDDSIGNITIEKGSITVNGVSLTVVNSKKNEFSVAIIP
                     YTYEHTNFNSFAIGTKVNLEFDLIGKYISKLYTSK"
     gene            complement(11667..11858)
                     /locus_tag="PLQ70_01165"
     CDS             complement(11667..11858)
                     /locus_tag="PLQ70_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33255.1"
                     /translation="MIYLLGVSIVFAGPNPPPPNPNKKPPPPPGLPIDENLFALFIIA
                     ILIGAYIIYRHNLKTKTPI"
     gene            complement(12106..12179)
                     /locus_tag="PLQ70_01170"
     tRNA            complement(12106..12179)
                     /locus_tag="PLQ70_01170"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12143..12145),aa:Ile,seq:cat)
     gene            complement(12325..12726)
                     /gene="mce"
                     /locus_tag="PLQ70_01175"
     CDS             complement(12325..12726)
                     /gene="mce"
                     /locus_tag="PLQ70_01175"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HQE33256.1"
                     /translation="MNKIEHIGIAVTSLETSTIIYEKLFGSAAYKQEEVVSEGVKTAF
                     FKNGPNKIELLEATNSESPIAKFIAKKGEGIHHIAFEVDDIVAEMARLEKEGFVLLNE
                     IPKKGADNKLVAFLHPKSTNGVLIELCQELK"
     gene            12862..13257
                     /gene="rbfA"
                     /locus_tag="PLQ70_01180"
     CDS             12862..13257
                     /gene="rbfA"
                     /locus_tag="PLQ70_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQE33257.1"
                     /translation="MEQTNRQKKIGGVIQKDLVDILQGEVRKNGVTNLIISVSKVSVT
                     TDLSVATVYLSIFPQEKAQETLVAIKTNSKLIKHDLSQRVRLQLRKVPNLVFFIDDSL
                     DYIEKIDNALSNKDNPIENRDLLEKRRFQ"
     gene            13261..14460
                     /locus_tag="PLQ70_01185"
     CDS             13261..14460
                     /locus_tag="PLQ70_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQE33258.1"
                     /translation="MNFPLYIAKRYIFSSSKNNAINIINRIASVGIIVGAMALFVVLS
                     VFSGLKIFSLSFSNDIDPDLKIRSTLGKSFLISASQETEIEQIEGVVSYSKIIEERVL
                     FTFDGKQQVTYLKGVDENFNQVNAIKKTIFSGDWLKKDTYQVVVGYGISNEFSMGLLD
                     YNHYLEVLAPRPGKGNIENPDEAFNKMTLIPVGIYAISEDLDSKYVFADLGLAQELLE
                     YKTNQISGIELKIKKDADENTIIGKLQNIFNNKITIKNRAQLNESLYKMLNTENIAVY
                     LIFTLVIIIALFNLIGALIMMILDKKGNLKTLLNLGTDIKDLRKIFLLQGTLLSVFGG
                     IIGLLLGVGLVLLQQEFELVMITPTLAYPVVFNIENVLIVLATIITLGFIASLIASSR
                     VSKKLLD"
     gene            complement(14607..14954)
                     /locus_tag="PLQ70_01190"
     CDS             complement(14607..14954)
                     /locus_tag="PLQ70_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HopJ type III effector protein"
                     /protein_id="HQE33259.1"
                     /translation="MTITTFLEKLKQTPNSITFPETIAIIEENYDFTPTTFDNGAQHN
                     EAGQNSGSCKLFAFAKLQNLSQAETLACFGAYYFEEVLDDPEGTNHQNIRNFMKLGWD
                     GIQFEGEALRLKV"
     gene            15122..16747
                     /gene="ade"
                     /locus_tag="PLQ70_01195"
     CDS             15122..16747
                     /gene="ade"
                     /locus_tag="PLQ70_01195"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013870864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HQE33260.1"
                     /translation="MQIQGQIVDIENRRIYAGEITVESGKITSIIEKKHEVQNYIMPG
                     FIDAHIHIESSMLVPSEFAKIAVLHGTVATISDPHEIANVLGSAGVYYMIENSKKAPL
                     KFHFGAPSCVPATTFETAGATIDSDGIKELMASPDIYYLAEMMNYPGVLFDDAEVLKK
                     IQWAKYFNKPVDGHAPGLRGEAAKKYISAGITTDHECFSFEEAEEKLSLGMKIIIREG
                     SAAKNFEALIDLLPENYENMMFCSDDKHPDDLILSHINSLCARAVAKGIDVFKILQVA
                     CVNPVNHYKMNVGLLKENDAADFIVVEDLTNFKVKQTYINGELVAKNGESFVKHIPFK
                     TPNNFNITSKEISDFTISGSASKIRVIEALEGQLITNEIYHRPLIVDGKIVSDTENDI
                     LKMAVVNRYENTKPAIAFIKNFGLKKGAIASSVAHDCHNIVVVGTSDEEICNAVNLII
                     KNTGGVCAVNGSENKMLPLPVAGIMSDKNGWETGKLYQEIDAMAKELGSHLKAPFMTL
                     SFMALLVIPDLKLSDKGLFSGNSFSFVDLEVED"
     gene            16951..17076
                     /locus_tag="PLQ70_01200"
     CDS             16951..17076
                     /locus_tag="PLQ70_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33261.1"
                     /translation="MNGYPTNIIVDKKGIIQFYEMSFRSDILEKMSSALDKCIAK"
     gene            complement(17083..18075)
                     /gene="dusB"
                     /locus_tag="PLQ70_01205"
     CDS             complement(17083..18075)
                     /gene="dusB"
                     /locus_tag="PLQ70_01205"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQE33262.1"
                     /translation="MIKIGNIELPDFPLLLAPMEDVSDPPFRRLCKEHGADLMYSEFI
                     SSEGLIRDAIKSRMKLDIFDYERPIGIQIFGGDEEAMALSSKIVSTVNPDLIDINFGC
                     PVKKVVCKGAGAGVLKDVDLMIRLTQAVIDSTHLPVTVKTRLGWDDDSINIDEVAERL
                     QDIGVAALSIHARTRAQMYKGHSDWSHIARVKNNPRITMPIFGNGDIDSPEKALHYKN
                     EYGIDGIMIGRAAIGYPWIFNEIKHYFKTGEHLAKPTVVDRVEAVRNHLTWAMEWKGE
                     RLGIVETRPHYTNYFKGIHSFKTFRQKLVTLDKPEELFAHLNEIEEAYSGYEVV"
     gene            18545..20341
                     /gene="lepA"
                     /locus_tag="PLQ70_01210"
     CDS             18545..20341
                     /gene="lepA"
                     /locus_tag="PLQ70_01210"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQE33263.1"
                     /translation="MKHIRNFCIIAHIDHGKSTLADRLLGATQTVTAREEKAQLLDNM
                     DLERERGITIKSHAIQMEYKYKGEEYILNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     AAQSIQAQTISNLYLALENDLEIIPVLNKVDLPSANPEEVSDDIIDLLGCKLEDIIHA
                     SGKTGFGVENILAAIIEKIPAPKGNKDEPLQALIFDSHYNPFRGIEVIFRVKNGEIKK
                     GQKIKFMATGNEYFADEVGTLKLNQVPKNVISTGDVGYLISGIKEAKEVKVGDTLTDA
                     KTPTTNMITGFEDVKPMVFAGIYPVDTEDFEELRASMEKLQLNDASLVFAAESSAALG
                     FGFRCGFLGMLHMEIIQERLEREFDMTVITTVPNVSYLAYTKKEPEVGFVVNNPSDLP
                     EPSRLDRVEEPYIKATIITKADFVGNVMSLCIEKRGIITNQTYLTTERVELNFDMPLA
                     EIVFDFYDRLKTVSKGYASFDYSPIGMRTSKLVKLDVLLNAQSVDALSALIHESNAYT
                     IGKKMCEKLRELIPRQQFDIPIQAAIGAKIIARETIKALRKDVTAKCYGGDISRKRKL
                     LEKQKKGKKRMRLVGNVEIPQEAFMAVLKLND"
     gene            complement(20515..20952)
                     /locus_tag="PLQ70_01215"
     CDS             complement(20515..20952)
                     /locus_tag="PLQ70_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33264.1"
                     /translation="MKKVSLLFIFAMFLTFANSFGQARKELNFGLVGINYEIPVHKDI
                     TIAPGVGTNFDLDWINLGVKGNYYFDNAFGISDSAWDVYGGLNVGYSIYNGDNDIDNS
                     SDLNLGLQVGGRWFWNDKWGIYLEIAGGNVSGMSPAIGLTVKL"
     gene            complement(21018..23030)
                     /locus_tag="PLQ70_01220"
     CDS             complement(21018..23030)
                     /locus_tag="PLQ70_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE33265.1"
                     /translation="MKKIITTLFIWISAILHAQETVEKKQDTIHKNKPFTLDEVIVST
                     AFNKIQSQNVMKVEHSTIKKLQQKGTATLIEGLATIPGVSQVSTGTSIGKPVIRGLNG
                     NRVLVYSQGVRIENQQFGDEHGLGLNDAGIESVEVIKGPASLLYGSDALGGVLYFNPE
                     KFANSGTFKANFSQNFFSNTLGSNSSLGLKTSTENWKFLARGTYNTHSDYKIANGDRV
                     TNTRYQETDFKTGIGYSNSKFSTVFRYNYNDLDLGIPEEGIAEQTTNKKTAYPKQGVF
                     NHLLSLNNTLFFEKSKLDVDLGYISNDRSEFEDSQVAVLHMKLNTFNYDAKYHLPKFG
                     KLESIVGVQGMHQTNTNSGEEYLIPDATTNDFGVFGTGHYQWESNVFQAGLRFDNRKI
                     TTTAHGVSGEEGSFEAIDKSFDSFNASLGYKTNLAKDLSLRLNVASGFRAPNLAELTS
                     NGVHEGTNRYEVGNPDLKTEQNVQTDLNLEYNNSHFEFFVNGFYNHINDFIYTSPTGI
                     VLDNNDVFEYIQNDAKLFGGEIGLHFHPHPLDWLHYETSFETVTGKKQDGDYLPLIPA
                     NNWNNTIRAEFKIKNWLEDSFASLNVSTTFNQNNVNDFETKSNGYTLLNLGFGGKVSI
                     GKTAFNVNLNGNNLLNKQYISHLSRLKTDSIPNIGRNIVLGLNFNI"
     gene            complement(23097..23447)
                     /locus_tag="PLQ70_01225"
     CDS             complement(23097..23447)
                     /locus_tag="PLQ70_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33266.1"
                     /translation="MKKKFVILNTFLALAVLFAMGFQSVHSFEHLAQQLSEKKCHHES
                     TSKQQITHQHHNLDHCFVCNFSLSSFIAADISSFEFQKTSFPSGYTFFKSREITQFFK
                     GSLFALRAPPVFIV"
     gene            complement(23539..>23827)
                     /locus_tag="PLQ70_01230"
     CDS             complement(23539..>23827)
                     /locus_tag="PLQ70_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HQE33267.1"
                     /translation="SEAVNVEFSNDFDVNEVRTILSQTEGIVVQDNLDTFTYPMPRYS
                     EGKNEVFVGRIRRDESQANTLNMWIVADNLRKGAATNTIQIAQYLVAHNLV"
BASE COUNT         7736 a         4064 c         4170 g         7857 t
ORIGIN      
        1 cgatggagtc ggatttaatg taaaaaaagc aaaaaaagga attggtttgc aaaacatgct
       61 ttctagagtg aatgaatgca aaggaatgtt tgatgtaaaa tcaaaaaaag gagaaggaac
      121 aactattaca gttacaatac ccatttaaaa aaaattaaaa aaaatgacat ttgattcccc
      181 agccgatgta acaattaaga ataatatatt aattgtagat gatcacccat ttatcattca
      241 aggttataaa aatgctataa ctcggtacaa tccaaaacaa tatgagtttc ttataactga
      301 ggcaaaagac tgtgaatcag gatataatgt aattacaaac ccagaaactt cgcctttcga
      361 cattgctttt ttagacatca gcatgccgtc ttacgacgag aaaggaattt tttcaggaga
      421 agatttagca aaattgttga atgagtacat gccaaattgc aaaatcattt tgcttaccat
      481 gtacacggaa ttgctgaaaa tcagaacaat aattgatgca ataaatcctt taggactagt
      541 tataaaaaac gacttaacat tcgatgagtt gctttttggc tttaataaag tcataaataa
      601 tgaaacttat tacagtcaat ctattcaaaa aatgttagat cagtccgaaa tggaagcgat
      661 tgaaattgac ctttttgaca aacaaattct ttttcacatc tcaaaaggga caaaaacaaa
      721 agatattcca caatatgttc caatttcttt aagtgccatc gaaaaaagaa aaatgaatct
      781 gaaaaaaata ttaagtatta ctgacgaaac tgatattgaa ttagtgagag tcgctaaaaa
      841 tagaggttta cttttttaaa ctattttaca agaaaagaaa acggcgcata atcagcgccg
      901 tttttggttt ttttattctt cactcaaagc attccatcca cgagctttca gcggaattct
      961 agtgttggca cgagttacca aatgaattcc ttcgctttct tgggtcatat gtccaataat
     1021 tgtgaaattt ggatttcctt ttattttttc aaaatcttcc attttaatcg tgaagagtaa
     1081 ttcataatct tcgccaccat tgattgctac agtggttgca tcaaggttaa attcttcgca
     1141 aacattcata aattgcggat caagaggcaa tttgtcttca tataaattac aacccacttt
     1201 ggattgtttg caaatatgca taatctcaga agacaatccg tctgaaatat ctatcattgc
     1261 tgttggttgt atttccaatg cgtgcaataa agtgcgaatg tctttacggg cttctggctt
     1321 taattggcgt tcaattaaat aggtataagc atccaaatcg ggttgcgaat tggggtttac
     1381 ctgaaaaact tgtttttctc tttctaaaac ttgtaatccc atgtaggcag cgccaatatc
     1441 tccagttact actaataagt cagtttgtcc cgctccattt ctatacacaa tttcattttc
     1501 atctgcttca ccaagaactg taatgctgat aatcatacct ttttgagaag aagtggtgtc
     1561 accaccaata acatctattt tatattcatt agcagctagc gaaattccgt cgaataattc
     1621 ttctaatgct tctagtggaa aacgatttga aactgcaatc gaaactaaaa tctgtgttgc
     1681 ttttgcattc atagcacaaa tatccgaaac attgactaca acagctttgt atcctaaatg
     1741 tttcaaaggc atataagcca aatcaaaatg aactccttcc accaaaatat ctgttgaaac
     1801 tacaactttt ttgtctgcaa aattcaaaac agcagcatca tcaccaatgc cttttaaggt
     1861 cgaagtttga ttgatttcaa agtttttggt caagtgttct atcaatccaa attcgcccaa
     1921 ttgagcaatg ctggttcgtt gtgggttttt atcttcaatc atagtattgc aggaatgaaa
     1981 caatcttcat tcattattta aattagtgtt gaggtgcaaa ggtacaaagt tttttagcca
     2041 cggatggcaa ggatttttac cgaaataaat cagtaaaaaa tcgttaatta cactttagta
     2101 ggaatgtgta atttaaatta tagcattttt ttaatagtta atcttgccaa tatgcctcat
     2161 tatacggaca atcttgtctt ttctacgatt aatataagaa gaagaactgg tcgctgaata
     2221 ttttcttgga ttcggcaaaa tagctgcaat tccagcggct tgaatttttg taagactctt
     2281 agcatctttt cggtaccaat gttgcgttgc agcttgggct ccataaatcc catttcccat
     2341 ttcgatgctg ttgagataga cttccataat gcgctctttg ccccaaatca gttcgataag
     2401 aatcgtaaaa taagcttcca aacctttgcg caaatagctt ctgccttgcc aaagaaaaac
     2461 gtttttggca gtttgttgcg aaatggtact tcctcctttt atccttctcc ctctttcatt
     2521 gtttttataa gctttttgca tggcggtaaa atcaaaaccg tgatggctca gaaaagttcc
     2581 atcttcactc gcaattacgg ctttttgcaa attaagtgaa atgttttcta tgggttccca
     2641 gtcgtggcta taataatttt ctttgccggc gagcttattt tctatggttc gaatcaccat
     2701 caaaggtgtg aatggaacgg gtacaaactt aaataatacc acaagaaaga tagacacccc
     2761 aaaaaaccac aacattgctt tgaataaaaa cctagagact ttgctcttga aagttcccga
     2821 ctcttttttt gctggttttt ttgttttttt cgatgttatt tttgtcgcca ttctgttaaa
     2881 actgattttt ttataaaagt aaaaaaaatt gcttcttatt tctaaaattt gctagaaata
     2941 acttcatcat attctgattg gattttgtat ttttaaaact ataataatcc caaatttata
     3001 atgaaaaaaa tagcttttat aatactgata atgatgaact taaatgcgat ggcgcaaaac
     3061 acaatgtccc cagaaatgct tttaaaatta ggaagaatat cggctctagg gctttctaaa
     3121 gatggaaaaa acataattta caaggtggac actccttcag ccgaagagaa taaatccaat
     3181 tcgaaattgt attctttgcc cataaatgga gggaatccaa ttgagattgc ttctgccaaa
     3241 gaagtgttga acgacaaaaa catttctcca gacggaaaat ttattgttta tgaccaagaa
     3301 gtgaaaatag acaaagttca cggcaaagat ttttatccag aattagacaa atcgaatgtt
     3361 caaatctaca atggtttgga ttatcgtcat tgggacaaat ggaacgaagg caaattcaat
     3421 cacgtttttt ataaagaaaa taaagaaggt gcagatggaa tagatatttt gaaaggtgaa
     3481 aatttcgata gtccgcaaaa accttttgga ggagacgaag attatatttg gtcgccagat
     3541 agcaaaagca ttatttatgt ttgcaagaaa aaagccggaa ccgcttatgc cattagcaca
     3601 aataccgatt tatatgaata taatttagaa accggaaaaa caactaataa aactgaaggc
     3661 aatttaggct atgacactca tccaacattt tctccaacag gaaatttaac ttggttgcaa
     3721 atgaaacgcg acggttatga agccgataaa aacgacatta ttgttcaatt caaaggaatc
     3781 aatatcaatt taacagccca ttgggatgga acagtagaaa gttttctttg gagtcctgac
     3841 ggcaaaaaag tatattttca agccccaata gacggaacaa ttcaactgtt tgaagtcgat
     3901 tttcctgggt taaccaagaa attaccacaa gtagttcagt tgactaatgg tgattttgat
     3961 gtttctggtt tgattgcttt atcgggtgat agtattttcg taactcgaac ggatatgaat
     4021 cacgctgccg aaatttattc ctataatttg cagcaaaaaa cttggcatca attgacgaat
     4081 gtcaatactg aaacctataa ttcattggcg ttaagcaaaa ccgaaagaag atatgtcact
     4141 acaacggatg gtaaaaaaat gttagtttgg gttattcttc ctccccattt tgacgcttcc
     4201 aaaaaatacc caactttatt gtattgccaa ggtggaccac aatcggcatt gacacaattt
     4261 tattctttcc gctggaattt ccaattaatg gcggctcaag gttatattgt tgtagccccg
     4321 aatcgtcgtg gaatgcaggg gcacggtgtg gaatggaacg aacaaattag caaagattgg
     4381 ggcggacaag taatgcagga ttacctttca gccattgacg atgttgccaa agaaaactat
     4441 gttgacaaaa accgtttggg ttgtgttggt gctagttatg gaggttattc agcgttttat
     4501 ttggctggaa ttcacaacaa tcgattcaaa actttcattg ctcacgacgg agttttcaat
     4561 acgcaaagta tgtttggtac taccgaagaa gtgttcttta attattggga ttttggtggc
     4621 gcttattggg aaaaagacaa tgcagtagcg caaaaagcct acactgtatt taatcctatt
     4681 aacaatgttc aaaaatggaa tacgcctatt ttaattattc aaggtggaaa tgattttaga
     4741 gttcctattg gacaaggcca agaagctttt caggcggcac aaatgcttgg aatacaaagt
     4801 agattccttt attttccaga agaaaatcat tgggttttga aacctcaaaa tgctttggtt
     4861 tggcagagag aattttataa atggttgaaa gaaactttgt aaattttcag gatacacttt
     4921 ctctttttct atcaataaag agaaattttt aggtaatttt atatggttta attactttta
     4981 ctaaaattaa ttgtaatcga taacatagat aattatgaaa acatcattta ttgctttttt
     5041 actgttttgt ggaatttgtt ttggtcaaaa caatacggat acaagccttt atatgaagtc
     5101 agataaggtc acctatctta ctgatatagg ttctgaatct ggcgatttat acaaaacaat
     5161 tggacaccac gggccagcaa ttgaaaacga atggatggca ctccgaattt atttcaatga
     5221 aaaagtagct attgatgtgt attccaaacc caatttgggg ctagaattga gaaaagcaaa
     5281 ttggtatcca actccagaac aacaaaaaga aggatgggga gctgattatt ataaagtgaa
     5341 tgcaaccgtt ggacttggag gtgttaaact ttgggatgga gaaaaagtag ttccgttaaa
     5401 tccagttagc aatcgtttag cacgagttgg aaaaacagac accacttctt ggatggaaat
     5461 gatttctaaa ggagtttcat acaaaggtca aaaagtagat attctagttc gagtgaccgt
     5521 tttctctgga aaaagagagg caaaagttga agctatttgt ttgagcaaaa cgaaagttca
     5581 gtttgtaacg ggtgtgaatt atttcaaaga tttcaaatca gataaaggtt ctaattacat
     5641 cgcagtttgg ggtatgcacc ctgaagatgt agctgcttca aaagttgaag ttggtgctgc
     5701 aattaaatac aacccaaaag attatttgaa aaattttgat gatggaactc aatatttatt
     5761 ggtttcaaag ccaaccaaac atttagaaac taccataatt tcgagttgtg caatcgaaaa
     5821 agatttaaac agttttgaaa aattggtggc atttttaaaa taaaaatggc tgtcattttt
     5881 ctagaaatta ttagagtttc attctacaaa cactaatttt gaatcgaata aacataaaaa
     5941 aactcgctaa atcagcgagt ttttatatat ataatatttt cttattctgg atcgttcatt
     6001 ttaaaagtat ccataaaagc agtggtgtaa tctccttcaa tgtattttgg atcgtccatc
     6061 aactgtctgt gaaacggaat ggttgttttg attccttcaa tcacaaattc atccaaagct
     6121 cttctcattt tgctaattgc ttcttcacgc gtttgagctg tggtaatcaa tttggcaatc
     6181 atcgaatcgt aatttggcgg aatagtataa cctgaataaa cgtgagtgtc caaacgaact
     6241 ccgtgaccac ctggcatgtg tagcgtagtg atttttcctg gtgaaggtct aaagtcatta
     6301 taaggatctt cggcattgat gcggcattcg atggcgtgca attctggtaa gtaattttta
     6361 cccgaaattg gaattccagc ggctaccata atttgctcgc gaattaagtc ataatcaata
     6421 acttgttctg taattgggtg ttctacttga atacgagtat tcatttccat aaagtagaaa
     6481 ttacggtgtt tgtcaaccaa aaattctaca gttcctgcac cttcatattg aataaattct
     6541 gctgctttta cggccgcttc tcccattttc tgacgcaatt catcggtcat aaatggcgaa
     6601 ggagtttctt ctgttaattt ttgatgacga cgttgtaccg aacaatctct ttctgaaaga
     6661 tgacaagctt tgccataagc atcacctaca atttgaattt cgatgtgtct tggttcttca
     6721 atgagttttt ccatgtacat cccatcattt ccaaatgcag ctgccgattc ttgacgagca
     6781 ctttcccatg cttttagcaa atcttcttct ttccaaacag ctcgcattcc ttttccacca
     6841 ccaccagctg ttgcttttaa catcactgga tagcccattt cttttgaaag tttttgtgct
     6901 tgttcaaaag attctaacaa accgtccgaa cctggaacac aaggtactcc cgcagctttc
     6961 atagtagctt tggccgaagc tttatcgccc attctgtcaa tcatttctgg tgcagcacca
     7021 ataaatttca cattgtgttc ttgacaaatt ttggaaaatt ttgaattttc agaaagaaaa
     7081 ccatatcctg gatgtattgc atctgcgttt gtgatttcgg cagcagcaat aatgtttgac
     7141 attttaagat aggacaaact gctaggagga gggccgatac aaacggcttc atcggcaaac
     7201 ttcacatgca aactttcagc atcagcagtt gaataaacgg ctactgtctt gatacccatt
     7261 tctttgcagg ttctaatgat gcgaagcgca atttctcctc tgttggctat tagtattttt
     7321 ttaaacatct attttgagtt atgagttatg agttatgagt ttttgaaatt atgattgata
     7381 aaaaccaatt cataatttat aattcataat ttataaatta attaagacgg atctactaaa
     7441 aacaaaggtt gatcgtattc aacaggagac atgtcatcaa ctaaaatttt aacgattttt
     7501 ccagacactt ccgattcgat ttcgttaaat aatttcatcg cttcaatcac acaaagaaca
     7561 tttccttttg cgatagtgct tccaacttca acaaacatag gtttgtctgg agatggtttt
     7621 ctgtagaaag ttccaataat tggagatttt atagtgatgt aatttgaatt ttctgctggt
     7681 gctgctggtg ctgcaggtgt tgcaactggc gcaatttgtt gaggtactac tgctggttga
     7741 agtgctggtt gagctgttgg catttgtagg taagtagctt cggtaacatt gccttctagt
     7801 gtagttctga tggtgatttt tacatcatcc atttctaatt taacttctgc aactccagaa
     7861 tttgctacaa atttgattag attttgaatt tcttttaaat ccatgattat ttgttttaag
     7921 ttttatttta ttttttgtcg taagcccatt ttaggtaaat agatccccaa gagaatcctc
     7981 caccaaaagc agcaaatatt attgtatctc cttttttaaa taaatgttca aaatcactta
     8041 gaactagtgg aagagtggca gaagtagtgt ttccgtatct ttcgatgttc attaatactt
     8101 ttgaatcttc taaattcatt cgatttgcaa tggcatcaat aattctttta ttggcttggt
     8161 gtggaaccaa ccattggaca tcattattgg tcaaattgtt gtttttcaaa atagtttcac
     8221 aagcatcggc catattggta acagcatatt taaaaacagt ttttccgtct tgcataatat
     8281 tatgctgatt gtctctaacg gtttcgaaag aagctggaaa aagagaacca cctgcagtca
     8341 ttttcaagaa ttctctgcct tcgccatctg ttcttaaata ttcgtcttgt aagcctaggc
     8401 cttcattgtt aggctcgaat aacacggcac cagctccgtc accaaaaatg atgcaagttg
     8461 ctctatctgt ataatctaca attgaggaca ttttgtcggc gccaatcaat agtacttttt
     8521 tatatcttcc tgattctatg taagctgcgg ctgttgacat accaaacaaa aaacttgaac
     8581 aagcagattg taggtcataa gaaaaggcat tggttgctcc gatttcggta gctacataag
     8641 ctcctgtcga tgctacaagc atatctgctg ttgctgttgc gactaagact aaatcaattt
     8701 ctaagggatc aatatttgat ttattaatta agtcttgagc ggcttttatt gctaaataag
     8761 aggttccttt ggcatcatcc ttcagaattc ttctttcttt tatcccagta cgtgtagtaa
     8821 tccactcatc attagtatcg actaatgttt ctagaatact atttgtaagt acatagtcag
     8881 gaacataggc tccaacagct gtgattgcgg ctgtgatttt attcatttta ttcaaatttt
     8941 tctagccaaa tgctattatt taaaattttt ctaaaggact cgaaaaatac aaaaaaaaat
     9001 caaatcattc tctttttgat agtccttatt taacataaat tataaacaac aaaaaactct
     9061 cacaaagtga gagttcaagt atcgtttaca gtattatatt aagcaacagc tacagattta
     9121 tctataacaa cttgccctct gtaatacatt tttccttcat gccagtaagc tctgtgatac
     9181 aaatgagcct cgcctgtgat agggcaagtt gctatttgag ctacagtagc tgtgtaatga
     9241 gttcttcttt tatctcttct tgttttcgag gtttttctct taggatgtgc cattttacta
     9301 tattatttat ccgttaatag ttgctttaat ttgtcccaac gtgggtcggt atcttcttgt
     9361 ttttgttctt ttgtatgctc ttttttcagt tcttttatgg ttaattcttt cagttttgct
     9421 aaggcttctg tatttaaact tccgtcttta acacctggat gaattcgtct tagaggaaca
     9481 gaaagaacaa tcatttcata aatatattgc gaaatatcta tttgaaactc tccgtgtggc
     9541 actatcaaca actcttcatt atcattgttg aaagcatctc caaaacggac gattaatttc
     9601 attttacctt ttatgggcaa atcaaattct tcgtttgtca tgtcgcaagg cacgtttacc
     9661 gttcctttat gcttgaaagc caactctaac attgtgcttt ttttctctaa aactacgttt
     9721 actttgatat ctgaatcatt aaattcgtga taatcaaaga tttcaaagaa cgccttattt
     9781 atttgatatt caaaatgatg tttccctagc tttaatccta taaaaggaat taaaaattct
     9841 ttcttcttgt ccatttcaac aacaatttgc cctaaacttc gggagtgcaa agatataaaa
     9901 ttattggaaa tctaataatc ttattaatct ttttttgttt atatctattt ttcttttgtt
     9961 ttcaatggtt tttggctgat ttcagcatac tgatttctgg aattatacac atctattgct
    10021 aaataaacgg cttctttgaa tgaattataa tcggcaattc ctttaccagc aatgtcatat
    10081 gccgttccat gatctggcga tgttctaatt ttatttaaac ctgctgtgta atttacacca
    10141 ttcccaaaag ataatgtttt gaacggaatt aacccctgat catgatatgt ggcgataata
    10201 gcatcgtatt tgtcatattg attgctacca aaaaaaccat cggcagcaaa aggaccaaaa
    10261 accaaagttc ctttatcgaa tatttttttc aatgctggtt ttaaaacagc atcatcttct
    10321 ttgccaatta cgcctccatc accacaatgt ggattcaatc ctaaaacggc tatttttggc
    10381 ttgttgatgc tgaaatcttg tataatcgtc tgctttatag tttcgatttt ctttacaatt
    10441 aattcttctg taagatggga tgcaacctca tttaaaggaa tgtggtctgt taacaaacct
    10501 actctcaagt tgtcttgtac catcaacatc aaggcattgc cttctagttc ttggtctaaa
    10561 taatcggtat gtcctggaaa tttaaaatct tctgattgga tgttgtattt atttattgga
    10621 gcagttacca atacatcaac aagaccttct tttaaagctt tagtggccgc aagaaatgat
    10681 tttatggcgt attttccaat tttttcatca tttacaccaa aattaatttc tactgcttct
    10741 tgccaaacat ttaaaacatt aatttttccc aaaactattt gatccaatct gtcgattcct
    10801 tgaagcatcg aagttgactc taaggttttt ttaataaacg acaccacttt tacattggca
    10861 aaaattacag gagtacacaa ttctaacatt cgagaatctt cgaaggtttt aagaatcact
    10921 tcgcttccaa taccgtttaa atctccaatc gaaattccaa cgattatatt ttctgctttt
    10981 tttatcatga catcacttat ttattgctaa ttttgaagtg caaatttagt aaaataaaat
    11041 aagaaatgtt tacaggaata atagaatccc ttggagtaat acaagaaatc aaaaatgata
    11101 aggacaatgt gcatattaca attgactcct caattacaaa tgaactaaaa attgaccaaa
    11161 gtgttgctca caacggaata tgtatgacgg tggtttcgat tgataatagc tcttataccg
    11221 taactgctat tgctgaaaca ataaaaaaaa caaatttatc ccatctaaaa gtaggggata
    11281 gcgtcaatct tgaaagagct atgaaattgg gtgatcgact cgacggtcat attgttcaag
    11341 gtcatgtgga tcaagtagga acgtgcaagg ttgcaaatga aacaaatgga agttggtatt
    11401 atacttttga atatgatgat tctattggaa atattactat agaaaaaggc tcgattactg
    11461 taaatggtgt tagtttgact gttgtaaatt ctaaaaaaaa tgaatttagc gttgctatta
    11521 ttccttatac atatgaacat acaaatttta attcctttgc cattggcact aaagtaaatc
    11581 ttgaatttga cctaattggg aaatatattt caaaattata tacgagcaaa taaagcgcat
    11641 ttattataaa aacaaaaact ccaattttaa attggagttt ttgtttttaa gttatgtctg
    11701 taaatgatat aagctcctat aagaattgca attataaaaa gggcgaatag attttcgtca
    11761 ataggcaatc caggtggtgg tggcggtttt ttatttgggt ttggtggtgg tggatttgga
    11821 cctgcaaaca caattgacac acccaatagg tatatcattg taattaaaaa cttgtttgca
    11881 ttaactttca tatttactaa aaaatcgttt tttaaagaaa aagaacctat caaatcccaa
    11941 tctaataact acatattcta taaaatagta tgtgcaaagt tatttttatt tactaacaaa
    12001 acaacacttt agtcgattaa ataccacatt ttaacgatta aataccatat tttaatattt
    12061 taacaaaaaa aaccccacat aatcattgat tgtgaggggt gtttgtggtc ccacttggaa
    12121 tcgaaccaag cacctactga ttatgagtca gttgctctaa ccgaatgagc tataggacct
    12181 ttttagcgat tgcaaaatta ctactatttt atatttatac aactattttt gagttagttt
    12241 ttttataacc ttactataaa tccctctttt aatacagaac taattgatta ttagagcact
    12301 ttaatatcaa aaaggattga aaaactattt caattcttga cacaattcta ttaaaacgcc
    12361 atttgtgctt tttggatgta aaaaagcgac taatttatta tcggctcctt ttttgggaat
    12421 ttcatttaaa aggacaaatc cttctttttc taaacgagcc atttcggcta ctatatcatc
    12481 tacttcaaaa gcgatgtgat gaattccttc tccttttttg gctataaatt tggcaattgg
    12541 actttctgaa tttgttgctt ctaatagttc aattttattg gggccgttct taaaaaaagc
    12601 tgttttgaca ccttctgaaa caacctcttc ttgtttgtaa gctgctgacc caaatagttt
    12661 ttcatatata attgtcgaag tttctaagct agtcacagca attcctatat gttctatttt
    12721 attcatattt ctcttgaatt tgaattttta aaaataatta aatttacgtt tttaaaatga
    12781 tcaagaacta aataaagtta aaaaatatag gtcttagcta ctttataaga ttgtctctca
    12841 aaaaattagt atttttgcac aatggaacaa acaaatagac agaaaaaaat aggtggagtt
    12901 atccaaaaag atttggttga tattctacaa ggtgaggtaa gaaaaaatgg agttaccaat
    12961 ttgataattt cagtgtcaaa ggttagtgtg actaccgatt tgtcggtagc tacagtgtat
    13021 ttaagcatat ttcctcaaga aaaagctcaa gaaactttag tggcaataaa aacaaattct
    13081 aaattaatta aacacgattt gtctcaaaga gtgcgcttgc aattacgaaa agtgcctaat
    13141 ttagtattct ttatagacga ttcattagat tatatcgaaa aaatcgacaa tgctttgtca
    13201 aacaaagaca atcctattga aaaccgtgat cttttagaaa aacgtagatt tcaataaaag
    13261 ttgaatttcc ctctttacat agccaagcgt tatattttta gcagtagtaa aaacaatgct
    13321 atcaatatca tcaatcgaat tgccagtgtg ggaatcattg ttggcgcaat ggctttgttt
    13381 gtggttttat cggtttttag tggattgaaa atttttagtc tttcgttttc caatgatatt
    13441 gatccagatt taaaaattag aagtacacta ggaaaatctt ttttaatttc ggcttcacaa
    13501 gaaactgaaa tcgaacaaat tgaaggagtt gtttcttatt caaaaatcat cgaagaaagg
    13561 gttttgttta cttttgatgg gaaacaacaa gttacctatt tgaaaggggt agatgagaat
    13621 ttcaaccaag taaatgccat aaaaaaaacc atttttagcg gagattggct caaaaaagac
    13681 acctatcaag tagttgttgg ttatggtatt tcaaatgaat tctcgatggg tttgttggat
    13741 tacaatcatt atttggaagt tttagcgcca agaccaggaa aaggcaatat cgaaaatcca
    13801 gatgaggctt tcaataaaat gacattaatt cctgttggga tttatgccat tagcgaagac
    13861 ttggattcaa aatatgtgtt tgctgattta ggtttggctc aagaattatt agaatacaaa
    13921 accaatcaaa tttcggggat agagttgaag attaaaaaag atgccgatga aaatacgatc
    13981 attggtaaac ttcaaaacat tttcaacaat aaaattacta taaagaatag agcccaactc
    14041 aacgaatctt tgtataaaat gttgaatact gaaaatatag ccgtgtattt aatatttaca
    14101 ttggtcatta ttattgctct tttcaatctt attggtgcat taattatgat gattttagac
    14161 aaaaaaggaa acctaaaaac gttattaaat ctaggaactg acatcaaaga tttgcgtaaa
    14221 atattcctat tgcaaggcac tttattgagt gtttttgggg ggattattgg acttctttta
    14281 ggtgttggat tggtattgct acaacaagag tttgaattag taatgattac accaacatta
    14341 gcttatcctg tggtttttaa catcgaaaac gtattgattg tacttgcaac aattatcaca
    14401 cttggtttta tagcatcgtt gattgctagt agtagagtga gtaaaaagtt gttggattaa
    14461 tattcatcaa aaaacgaatt ctaaactcga gtgaaagcaa atttaagatc accatttgta
    14521 gatttatttt tttcaatttg aaattacgag caacgaagca atctcataac tgagtgaaat
    14581 tgcaattgct tcgttctaca atgaatttag accttcagcc tcaaagcttc tccttcaaat
    14641 tgaattccgt cccatcctaa tttcataaaa ttacgaatat tttggtggtt tgttccctct
    14701 ggatcgtcca atacttcttc aaaataatag gctccgaaac aagctaaagt ttctgcttga
    14761 gataagtttt gcaatttcgc aaaagcaaat aatttacaag aacccgagtt ttgccctgct
    14821 tcattatgct gcgctccatt atcgaaagtg gttggcgtaa aatcgtaatt ttcctcaatt
    14881 attgcaatag tttctggaaa agtaatggag tttggtgttt gttttagttt ttctaaaaaa
    14941 gtggtaatgg tcattatata atttattatt tgaattattt ctcaccgcaa attcgcaaat
    15001 ttttaattta ttatcaaaat ttaaagacta aaatttgaaa aaaatctgcg aatctctggt
    15061 aaaacttaat aagctaaatc aaaagattgt acttttgatt attattcaaa atcaatccaa
    15121 aatgcaaatt caaggacaaa ttgtcgatat cgaaaaccga cgtatttatg ctggcgaaat
    15181 cactgtcgaa agcggaaaaa taacttccat aatcgagaaa aaacacgagg tgcaaaatta
    15241 cattatgccc ggatttatcg atgcccacat tcatatcgaa agttcgatgc ttgttccttc
    15301 agagtttgca aaaattgccg tgcttcacgg aactgttgcc acgatttctg atccacacga
    15361 aattgctaat gttctgggtt cggcaggcgt ttattatatg attgagaaca gtaaaaaagc
    15421 cccgttgaaa ttccattttg gcgcaccgtc ttgtgttcct gcaaccactt tcgaaactgc
    15481 cggagcgaca atcgattcag atggaatcaa ggaattaatg gcttctcccg acatttatta
    15541 tttggccgaa atgatgaatt atcccggcgt tttatttgat gatgctgaag ttttgaaaaa
    15601 aattcaatgg gcaaaatatt ttaacaaacc agttgatggt cacgctccag gattgagagg
    15661 tgaagcagcg aaaaaataca tttcggcagg aattacaacc gaccatgaat gcttcagttt
    15721 tgaagaagcc gaagaaaaat tgtctcttgg aatgaagata attattcgtg aaggaagtgc
    15781 cgccaaaaac ttcgaagcac tcatcgattt gcttcctgaa aattacgaga atatgatgtt
    15841 ttgttcagat gacaaacatc ctgacgattt gattttaagt catattaact ccttgtgtgc
    15901 tcgtgctgtt gccaaaggga ttgacgtttt caaaatcttg caagtcgctt gcgtgaatcc
    15961 tgtgaatcat tacaaaatga acgtgggttt attgaaagaa aacgatgctg ctgattttat
    16021 tgtggtcgaa gatttgacta acttcaaagt aaaacaaacc tatatcaatg gggaattagt
    16081 tgctaagaat ggagagtctt ttgtgaagca cattccattt aaaactccta ataatttcaa
    16141 tattacttcc aaagaaatta gtgattttac tatttcaggt tcagcttcaa aaatcagggt
    16201 aattgaagct ttggaaggac aattgattac caatgaaata tatcatcggc ctttaattgt
    16261 tgacggaaaa atagtttcag ataccgaaaa tgatattctt aaaatggctg ttgtcaatcg
    16321 ctatgaaaat acaaaacctg ctattgcttt catcaagaat tttggattaa aaaaaggtgc
    16381 gattgccagt tccgtggcgc atgattgtca taatattgta gtagtgggaa cttccgacga
    16441 ggaaatctgc aatgcggtga acctgattat taaaaacaca ggcggagttt gtgccgtgaa
    16501 tggttccgaa aacaagatgt tgccgttgcc cgttgccgga attatgagcg acaaaaacgg
    16561 ctgggaaaca ggaaaattat accaagaaat tgacgcaatg gctaaagaat taggaagcca
    16621 tctgaaagca ccgtttatga cgctttcgtt tatggctttg ctcgtaattc ccgacctgaa
    16681 attatccgac aaagggttgt tcagcggaaa tagtttttcg tttgtggatt tggaagtgga
    16741 ggattaaccg caaagacgct agggcgcaaa agattatatt gaattgtttt tatcctttca
    16801 acaaaaaaaa actcattatt agatctcgtt atttgattgc tactcttcgg aaagaaaaaa
    16861 aagaaattta attattctat ttcactacat aaaaaaattg cactaattct taaaatatcc
    16921 taataaaaaa cgcccataaa aattcattta atgaatggat atcctaccaa tataattgta
    16981 gataaaaaag gaatcatcca attttatgaa atgagtttta gatccgatat tttggagaaa
    17041 atgtcttcgg ctttagacaa atgtatagcc aaatagccta aattaaacta cctcataacc
    17101 agaataagct tcttcaattt cattcaaatg ggcaaataat tcttcaggtt tatctaatgt
    17161 caccaacttt tgtctaaagg ttttaaaaga atgaattcct ttgaaataat tggtataatg
    17221 cggacgagtt tccacaatac cgagacgttc gcctttccat tccattgccc aagtgaggtg
    17281 attgcgaaca gcttcgactc tgtcaacaac ggtaggtttg gccaaatgtt cgccagtttt
    17341 gaagtaatgt tttatttcgt tgaaaatcca aggataacca atggcggccc gacctatcat
    17401 aatcccatcg ataccgtatt cgttcttgta atgcaaagct ttttcgggcg aatcaatgtc
    17461 tccatttccg aaaataggca tcgtgattct ggggttgttt ttcactcttg caatgtgcga
    17521 ccaatcggaa tggcctttat acatttgggc acgagttcgg gcgtgaatgc tcaaagctgc
    17581 aacaccaata tcctgcaaac gttcggcaac ctcatctata ttgatggaat cgtcatccca
    17641 acccaaacgt gtttttaccg taaccggcaa gtgcgtactg tcgataacgg cttgcgttaa
    17701 gcgaatcatc aaatcaacat ctttcaaaac tcctgcgcca gcacctttgc aaaccacttt
    17761 ctttacggga caaccaaaat taatatcaat taaatccgga tttaccgtgg aaacaatttt
    17821 tgatgacaat gccattgctt cttcgtctcc gccaaaaatc tggattccta tagggcgttc
    17881 gtaatcgaaa atatccaatt tcattcggct tttgatggcg tcacgaatta atccttcaga
    17941 agaaataaat tcagaataca tcaaatcggc tccgtgttct ttgcacaatc tgcgaaatgg
    18001 cggatcgctc acatcttcca taggtgcgag aagtaacggg aaatcaggaa gttctatgtt
    18061 gccaatctta atcatcttct gaaaattttt gcaaaattac ggttttttta atgaaagaag
    18121 aaaaagagaa ttgcaaaggg ttatttttca aggagtttaa gagatgtaaa taagattcaa
    18181 gtgggttaca gattacacgg attttatttc gcaaagattc ccaaagaagt cacggaggta
    18241 caccaagatt aaaaaaactc tgcgaatctt tgtgtttgct ttgggaatct ttgtgaaaca
    18301 aaacaacgac ttgttttata gccacttgac aaatagtgta ctcaataaaa acgtttccct
    18361 tgagtttgtg gttgctaggc tttgaaactg ttgaatttaa actatatttg cagattaatt
    18421 gcctgcgtga gggatagaag cggcatcctt ttgtggagtc ccgacagtaa tcgggacgga
    18481 acaaaagata cagcggaaag cccgaccctt gtggtcacgc ccaaaaaatt gacacaaaga
    18541 gagaatgaaa cacataagaa atttttgcat tattgcacac attgaccacg gaaaaagtac
    18601 acttgccgac agacttcttg gggcaaccca aaccgttacg gctcgtgaag aaaaagcaca
    18661 attgctggac aatatggact tggaacgcga acgcgggatt accataaaaa gtcacgccat
    18721 ccagatggaa tacaaataca aaggcgagga atatatcctg aacttgattg acactcccgg
    18781 acacgtggat ttttcgtatg aagtttcccg ttcaattgca gcttgcgaag gtgctctttt
    18841 gattgtggat gccgcacaaa gtattcaggc acaaacgatt tcgaatttgt acttggcatt
    18901 ggaaaatgac ttggaaatta ttcctgtttt gaataaagta gatttaccaa gtgcgaatcc
    18961 tgaggaagta agcgacgata ttattgattt attaggctgt aaattagaag atattattca
    19021 cgcttctgga aaaactggtt ttggtgtcga aaatattttg gccgccatta tcgagaaaat
    19081 tccagcaccc aaaggaaata aagacgaacc tctacaagct ttgattttcg attcacatta
    19141 caatccgttt cgtggaatcg aggttatttt ccgtgtgaaa aacggagaaa tcaagaaagg
    19201 tcagaagatt aaatttatgg ctacaggcaa tgaatatttt gccgacgaag tgggaacttt
    19261 aaaactaaac caagttccaa aaaatgttat ttcgacaggc gatgttggtt atttaatttc
    19321 tggaattaaa gaggctaaag aagtaaaagt tggtgatact ttgaccgatg caaaaacgcc
    19381 aaccaccaat atgattacgg gtttcgaaga tgtaaaacca atggtttttg ctgggattta
    19441 tccagtagat accgaagatt ttgaagaatt aagagcttcg atggaaaaac tacaactgaa
    19501 cgatgcttca cttgtatttg ctgctgaaag ttcggcggct ttaggttttg gtttccgttg
    19561 tggattcttg ggaatgttgc atatggaaat catccaggaa agattggaac gcgagttcga
    19621 tatgactgta attactacag ttcccaacgt ttcctattta gcttacacca aaaaagaacc
    19681 cgaagttggt tttgtagtta acaatccatc ggatttgcca gaaccttctc gtctagatcg
    19741 agttgaagaa ccttacataa aagctactat cattacaaaa gctgattttg tggggaacgt
    19801 aatgagtttg tgtatcgaaa aacgtggcat tatcaccaat caaacctatt tgacaaccga
    19861 gagagttgaa ttgaatttcg atatgccatt ggccgaaatt gtattcgatt tttatgatag
    19921 attgaaaacc gtttccaaag gatatgcttc gtttgattat tctccaattg gaatgcgaac
    19981 ttctaaatta gtaaagctag acgttttatt aaatgcccag tccgtggatg cgctttcggc
    20041 cttgattcac gaaagtaatg cttataccat tggtaaaaaa atgtgtgaaa aattgcgtga
    20101 actgattccg agacagcaat ttgatatccc tattcaggca gcaattggcg caaaaattat
    20161 tgcgcgcgaa accataaaag ccttacgtaa agacgttacc gccaaatgtt atggtggaga
    20221 tatttcccgt aaacgtaaat tattggaaaa acagaaaaaa ggtaaaaaac gtatgagact
    20281 ggttgggaat gttgagattc cacaagaagc gtttatggct gttttgaagt tgaatgatta
    20341 aattttagac ttcttagata aaagacggat agataataga gaaaacctga tgatgaaaat
    20401 tgtcaggttt ttttatattg gtaatgtggt caaaaatggt tggtggattt tcaaaaacaa
    20461 aaaaaatgcc cgagaaaatt ctcaggcatt tgatataaaa taagttgtga tattttacaa
    20521 ttttacagtt aatccaatgg caggagacat tcctgaaaca tttccacctg ctatttctaa
    20581 ataaatgccc catttgtcat tccaaaacca gcgtcctcca acttgaagac caaggttaag
    20641 gtcgctacta ttatcaatat cattatctcc attgtaaata gaatatccaa catttaaacc
    20701 accgtacaca tcccaagcag aatctgaaat tccaaatgca ttatcaaagt agtaattacc
    20761 ctttacacca agattgatcc aatctaagtc aaaattagtc cctacacctg gggcaatagt
    20821 aatatcctta tgaacaggaa tttcataatt aatgcctaca agaccaaagt ttaattcttt
    20881 gcgggcttgt ccaaaactat ttgcaaaagt taagaacatt gcaaaaataa ataataaaga
    20941 cacttttttc atataatttt tgtttaaagg ttgtttacaa atatagtcct atttcttgac
    21001 aaaaacaagg taaaatctta gatattaaaa ttcaaaccca gaacaatatt tcttccaata
    21061 tttggaatac tgtcggtttt taaacgagaa agatgagaga tatattgctt gtttagcaaa
    21121 ttatttccat tcaaatttac gttaaaagct gtttttccaa tacttacttt tcctccaaaa
    21181 cctagattca acaaggtata accatttgat ttagtttcga aatcatttac gttattttga
    21241 ttgaaagtgg ttgaaacatt taaactagca aaactatctt ccagccaatt tttaatttta
    21301 aattcggctc taatagtgtt gttccaatta ttggcaggaa ttaaaggcaa ataatcgccg
    21361 tcttgtttct ttccagtaac ggtttcaaaa ctggtttcat aatgcaacca atctaatgga
    21421 tgagggtgaa aatgcaatcc aatttctcca ccaaacaatt tagcatcatt ttgaatatac
    21481 tcgaaaacat cattattgtc tagtacaatt ccagttggcg aagtataaat aaaatcgttg
    21541 atgtggttgt aaaatccgtt tacgaaaaat tcgaagtggc tattattata ttccaaattt
    21601 aggtcggttt gaacattttg ttccgttttt aaatcaggat ttcccacttc atatcggttg
    21661 gttccttcgt gaactccgtt tgaggttagt tcggccaaat tgggcgctct aaatccagaa
    21721 gctacattca atcgtaatga taaatctttg gctaaattgg ttttatatcc caaagaagca
    21781 ttaaaactat caaacgattt gtcgatggct tcgaatgaac cttcttcgcc tgaaactccg
    21841 tgtgcggttg tggttatttt tctattatca aaacgcaatc ctgcctgaaa aacattcgat
    21901 tcccattgat aatgtcctgt tccaaaaacg ccaaaatcat tggtagtggc atcgggaatc
    21961 aaatattcct ccccagaatt ggtgttggtt tgatgcattc cttgaactcc aactatggat
    22021 tctaattttc caaattttgg caaatggtat tttgcatcat aattgaaagt gttcaatttc
    22081 atgtgcaata cggcaacttg actatcctca aattcacttc tgtcattgga aatgtaaccc
    22141 aaatcgacat ccagttttga tttttcgaaa aacaaagtat tatttaagct caataaatga
    22201 ttgaaaacgc cttgttttgg ataagctgtt ttcttattgg tggtttgttc tgcaatgcct
    22261 tcttcaggaa ttcctaaatc taaatcatta taattgtagc gaaaaacagt cgagaatttc
    22321 gaattgctgt agccaattcc agttttgaaa tcggtttctt gataacgagt gtttgtaact
    22381 cgatcgccat tagcaatttt ataatccgaa tgcgtgttat aagttccacg tgctaagaat
    22441 ttccaatttt cggttgaggt tttcagtcct aatgacgaat tacttcccag tgtattagag
    22501 aaaaaattct gactgaaatt ggctttaaaa gtacctgaat tggcaaattt ttcaggatta
    22561 aaatacaata cacctcctaa agcatccgaa ccataaagta aggaagctgg tccttttatg
    22621 acttccacac tttcgattcc agcatcattt aatcctaaac catgttcatc accaaattgc
    22681 tgattttcga ttcgaacgcc ttgcgaatag actaaaactc gattgccatt caaaccgcga
    22741 ataacaggtt ttcctataga agttcctgtt gaaacttgtg aaactcccgg aatagttgct
    22801 aaaccttcga ttagagttgc agttcctttt tgttgtaatt tcttgatggt cgaatgttcc
    22861 actttcatca cattttgtga ttgaatttta ttgaaagctg ttgaaacaat cacttcgtct
    22921 agtgtaaaag gtttgttttt atgaatagtg tcttgttttt tttctacggt ttcttgtgca
    22981 tgaagaatag cagaaatcca tataaataag gttgttatga tttttttcat ttgaaatgat
    23041 tttattgtta aatacgagaa ttgcacaaaa acttaatgtt tttgcgttag aataatttaa
    23101 acaataaaaa caggaggagc tcgaagcgca aataagcttc ctttaaaaaa ctgggtgatt
    23161 tctctggatt tgaaaaaagt atatccagaa ggaaagcttg ttttttggaa ttcgaaactc
    23221 gaaatatcag cagcaataaa actgcttaac gaaaaattac aaacaaaaca atgatctaaa
    23281 ttgtgatgct gatgtgtgat ttgctgtttg gaagtagatt cgtgatggca ttttttttca
    23341 gaaagttgtt gtgccaaatg ctcaaaacta tgcacggatt gaaaccccat tgcgaacaat
    23401 acagctaatg caaggaaagt atttagtatg acaaatttct ttttcatccg aggcaaaggt
    23461 aggtattgaa aaagaaaaat ccgattatct ttaaaaattt aaagttaatc agatttttta
    23521 taaaactgta aaaaggtttt acaccaaatt gtgtgctacc aaatattgtg caatttgaat
    23581 agtatttgtt gcagctcctt ttctcaagtt atccgcaaca atccacatat ttaaagtatt
    23641 ggcttggctt tcgtcacgac gaattctacc aacaaatact tcatttttac cttcagaata
    23701 tcttggcatt ggataggtaa aagtatctag gttatcttga actacaatac cttcggtttg
    23761 gctaagaatt gttctaactt cgtttacatc aaaatcattg ctgaattcca catttacggc
    23821 ttcgcta
//
LOCUS       DAOSNN010000011        23804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_2149_length_23804_cov_4.231673, whole genome shotgun sequence.
ACCESSION   DAOSNN010000011 DAOSNN010000000
VERSION     DAOSNN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 23804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23804
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2149_length_23804_cov_4.231673"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3932
                     /locus_tag="PLQ70_01235"
     CDS             <1..3932
                     /locus_tag="PLQ70_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQE33268.1"
                     /translation="DSKNRFWVGCINGLQQFNRQTNQFDDIKLLSANNQLLSTHITSI
                     IESKDGNIWISTSGEGVLRMTKDRDKTFRSVTYLNSKLLNQYLTFIFQDSSGKFWIGS
                     ENYGLNLYDPDKDKMYVFSVQNNKIGSNQISAITEDSKKNIFVGTLSGGLYKFNKNDF
                     SFISIKDANGNSNQPVKTIFLDKNNRLLVGTDGQGLKTYNYQKNLLEDMEIKSAPFDF
                     SKTKVHAILQDRAGNLWFGLFQKGVFLVRNNPYAFNYIGHKSFSQNLIGSNSIASILQ
                     DRNNELWVGTDNDGMYRLEKNFKNSVHYNPPAVPNIVSSIVELDNGEMWLGSFSQGLA
                     SFNKNTGKSTYFNNIPSSFQYNNLAIKINCMIKDQNDNLWIGTHGGGLFVFDPSTRKY
                     IKHFGSDVISNDWISSLFMDKDQRIWIGTYDNGINLVEENQGKISFIHNFKNYPFEES
                     SKVRCLSEGYKGQIWVATTNGLVTFNPDSYNPNQIKFSRYVKSATDKSSLNSNDVLFI
                     CKDSEDKIWLSTAGGGLNLAMETQNNQLKFKNFTKENGLPSDFILSMVEDDEKNLWLA
                     TENGISKFNIKHQTFRNYDTYDGLLKTRFSEASSLKLQNGNLIFGCKIGYLNFNPQTI
                     KNQKSTTQMVFTNFEVNNKISNVKSAEFPLKQDINYDNNIELDYQHNTISIYYTVLDY
                     RSNNKQLYAYRLKGLEDTWHQVKNQKKATFTNLDPGDYEFEVKCLNTDLYTNVPQKKL
                     SFTITPPFWKTNWAYFLYAIIFLALLEMSRRIVYSMIQLRNKVVVEHQMTELKLSFFT
                     NISHELRTPLTLIVNPIEEIAKNENLSPLGNEYIETVRKNADRLVRFVNQLLDFRKVQ
                     SGNEELHIAPIEMVTFINETITLFVHIAHEKNIKIQTKSNFGSINANLDKEKIDIVIY
                     NLLSNALKFSPNDSVITVELALEKDNFLKIYVVDQGTGVDENKLEDIFKLYYETNTGK
                     NKNVGTGIGLALCKEYVQLHQGSIYAVNNTDGGLTVAITIDLNNELLQSHSNTIASVA
                     QKNQKQPLVIAQNNENVAVENDTNLPLVLIVEDNNELRKFLKSQLQCFYRIIEAENGK
                     VALEIATKKLPDLILSDIMMPEMDGIELLDALKNSEETSHIPVVLLTAKSSVESKIEG
                     LNYGADYYITKPFDTDFLLASIENLIKQRKKFFENLQANVKTMALEPSEIIITTKDEQ
                     FLKDSIAIVENNLTNPKFNIDVISNSLSISRVTFNRKFKSLTNMTPVEFVREMRIKRA
                     KQFLDAGETDIAGIAYKVGFNDAGYFSTCFKEYYKIAPSDYLKQKQE"
     gene            4077..4889
                     /locus_tag="PLQ70_01240"
     CDS             4077..4889
                     /locus_tag="PLQ70_01240"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQE33269.1"
                     /translation="MWEQVQETVSFIKEKTNFNPEYGVILGSGLGSFTDDIAIEFTLP
                     YGEIPNFPVSTVHGHKGALVFGTIGNKKVVAMQGRFHFYEGYSMKEVTFPVRVMKYLG
                     VEKLIVSNASGGVNPNYEVGSIVILKDQINMMPEHPLRGKNDERFGPRFLNMSEPFSI
                     NMIAVAKALAKNLNITVHDGIYLGLQGPTFETLAEYKMVKILGADCVGMSTVPEIIVA
                     RHMDMECFGLSVITDMGNEENIETVSHDEVLEAAKKAEPNVRSLIKELILNY"
     gene            complement(4894..7068)
                     /locus_tag="PLQ70_01245"
     CDS             complement(4894..7068)
                     /locus_tag="PLQ70_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQE33270.1"
                     /translation="MKSILFFFLFVSIFSFSQKKKPVEKIDSIAYYNNLRDVNVKANK
                     YKDALHYTQKAIQYSKINHNTDALINENFSLGKLYYDVKKYDDALKYFNNSLTLSKTN
                     TPNSLQATAYYYLGMCFMKKRSYTNAKYNFDQAESLYNTLEIIDHADLINLQRGIIYK
                     SDKKYDLASTLFNQIIVKPDSPSILDSKSEALYQIGTIEMAQNRNNLALTYFNKALAL
                     NSKSKNLEQKSAILLALSTVYEKMLDRNSAYSYLKQHTNLKESISIIDNEKLGVDDYE
                     NFKESERLKEIKQINEEAKQQEKASKFSKLISILAIALISILSLLSLSLYKNNIIRTQ
                     SNKLLEEKNKELIIAKEKVEKASNARSEFLSTVSHELRTPLNAINGITHLLLEENPKK
                     SQMQYLESLQYSGNYLTKFINDILEINKIDSSKIQIEHINFNLKQLVENIQSSLKELA
                     VENDNSFNFNIDCEIPNYLIGDPTKLSQILMNLINNALKFTHDGEVTVTTKLVSVRDK
                     KAFVYFEVKDNGIGIPEDKFESVFDSFSQGSIDINRKYGGTGLGLTIVRKLVRLLGGK
                     INLESKVNEGSTFSFELPFKISKRVQDTDKKETTIDTIAFVNKKILVVEDNKINQMIT
                     KKMLNNKGIECEIIDNGEEAIEACKTNQYDLILMDVHLPGINGTIATQEIRKFDTKTP
                     VIALTAISLNENREMLLSYGMNDVITKPFVPEDFYIAIAKYI"
     gene            complement(7119..8111)
                     /gene="gap"
                     /locus_tag="PLQ70_01250"
     CDS             complement(7119..8111)
                     /gene="gap"
                     /locus_tag="PLQ70_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQE33271.1"
                     /translation="MKTRIAINGFGRIGRNLFRLLLNHPTLEVVAINDIADKKTMAHL
                     IKYDSIHGVLPSIVSSDEKGIIVDGKHYLFFHEKSISKLDWKSANIDVVVESTGKYKT
                     FDEINAHILAGAQKVILSAPSEVDTIKTVVLGVNEHILDGTETIISNASCTTNNAAPM
                     MKIINELCGIEQAYITTIHSYTTDQSLHDQPHKDLRRARGASQSIVPTTTGAAKALTK
                     IFPEFEGKIGGCGIRVPVPDGSLTDITFNVKRAVSIEEINAAFKLASETNLKGILAYT
                     EDPIVSVDIIGNRNSCLFDAQLTSVIDKMVKVVGWYDNEIGYSSRIIDLIILTT"
     gene            complement(8180..9049)
                     /gene="lipA"
                     /locus_tag="PLQ70_01255"
     CDS             complement(8180..9049)
                     /gene="lipA"
                     /locus_tag="PLQ70_01255"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQE33272.1"
                     /translation="METVLENTLPVGKPKWLRVKLPIGQKYTELRGLVENYKLNTICT
                     SGSCPNMGECWGEGTATFMILGNICTRSCGFCGVKTGRPETVDWDEPEKVARSIKIMN
                     IKHAVITSVDRDDLKDGGSIIWIETVKAIRRMNPNTTLETLIPDFQGIERNIDRIVEA
                     NPEVVSHNMETVRRLTREVRIQAKYDRSLEVLKYLKEKGIRRTKSGIMLGLGETEEEV
                     FETMRELYAAKVDIVTIGQYLQPSKKHLPVKEFITPEQFAKYEAFGLELGFRHVESGP
                     LVRSSYKAQKHIL"
     gene            9256..9810
                     /locus_tag="PLQ70_01260"
     CDS             9256..9810
                     /locus_tag="PLQ70_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HQE33273.1"
                     /translation="MSAMWYECKVKYRKTDEIGNQKVTTEPYLVDALSYTEAESRINE
                     EMKAYISEEFKITNIKVANYAEIHPFENADRWFKSKVSLMAYDEESGKERKTNMYVLV
                     QANDVKEAYDNTIEVMKGTMGDYTIPAISESPIVDVFPYFSGDEDELEQLEKFNALKA
                     SKPEKATEIEDHMEFVDEEEEVVS"
     gene            complement(9929..10870)
                     /locus_tag="PLQ70_01265"
     CDS             complement(9929..10870)
                     /locus_tag="PLQ70_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33274.1"
                     /translation="MKKSVSLIIMLLTFYISSAQIKTVFWRVDNLTQIGGKSVTVSGS
                     PKVIQTELGTAVEFDGIKDGLLVNDNPMNGATEFTIEMILKPYSGGAVEQRYLHFQQD
                     DNNRILAELRNNNNLNWSLDTFIKSGTSNQTLLDYSLVHSMNNWVHIALTYKNGVMTS
                     FVNGIQELIGAVSYQVVNSGQTSLGVRMNQVAWFKGAIHSVKVTHEVLNPSRFMKTTD
                     FLGLQNLEKNQLTSQISTNPMVTSTLLKYQLEESSNVSIKLFTIQGKEAISLFDGYKN
                     AGNYELKINRDYLKAGVYLVVIHSGNKKSVHKLIINP"
     gene            complement(11003..11302)
                     /locus_tag="PLQ70_01270"
     CDS             complement(11003..11302)
                     /locus_tag="PLQ70_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HQE33275.1"
                     /translation="MNGKIKYEFSEKSWQHSSPSGWYFVALPKEMSKEIRSILKFQEE
                     GWGRLKAVAKIGKSEWKTAIWFDTKINTYLLPLKAEIRKKENISAEKEIDVTIWL"
     gene            11552..14425
                     /gene="galA"
                     /locus_tag="PLQ70_01275"
     CDS             11552..14425
                     /gene="galA"
                     /locus_tag="PLQ70_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalA"
                     /protein_id="HQE33276.1"
                     /translation="MKNTLKTPFLLLIFFSFCLSVFSQSGSKEQNRDHISLDKDWRFA
                     FGHPSDTKKDFNTGIAYFSYLAKAGFGDGAANANFDDRSWRKLDLPHDWAVEQGFSKD
                     ASFSHGFKAIGRNFPDKSIGWYRKKITIPQSDLGRRIHLAFDGVFRNSIVWINGHYLG
                     NQDSGYLGFEYDITDYINYGGENTIAVRVDATMEEGWFYEGAGIYRHVWLNKSNELHI
                     PQNGTFVKTKTKDNTTTVNALVTVANDGKASKSFQIIQTIKDATGKKITEKTINSCSL
                     NPRETKDFTCDLTVENATLWSIENPYLYQMTTSIFEDEKLVDTFETTFGIRTIRFDAN
                     EGFFLNEKHIKIKGTNNHQDHAGVGAAMPDALQDFRIKTLKSFGSNAYRCSHNPPTPE
                     LLEACDRLGMLVIDENRLMGSTQNQLNDVKKMIERDRNHPSIISWSVGNEEWGIENDI
                     VGARIASSMQSYVKSIDATRPATAAFSGGIGSNGITTVMDLLGINYIANKSTDKQHQL
                     FPNQCLWGTEEGSTNATRGEYYRDNQKRIMPAYDKAPSASFISIENGWKHYNSRPYLA
                     GMFVWTGFDYRGEPTPFDFPSVGSYFGMVDQCGFYKDTAWYLKSWWQNQPVLHLLPHW
                     NWTGKENQSIEVWAYSNCDEVELFLNKKSLGKKTMQKDSHLEWNVSYSPGTLEAIGYK
                     NGKKIITDVVKTTGAAAILNLESNKNSLEASKNDIALITVSAKDKSGLHVPIADNEVS
                     FTISGPAKIIGVGNGNPTSLEADKYLETITVLNIENLKEKMVADFNVSDETKENVATD
                     SWQKAFTDDRNAAFGEKVKNIVYRTEFELPANYTESTINFFYNSLGKQQSIFINGKKI
                     ADAIPENKHGDTFLLDKTMLHSGKNTLAIVATPLLKVNSWDVVNQNPGTIQLITKADN
                     YKRKLFNGLAQVIIQTTGEAGEVKLSATANGLKSTEIIIKTVQ"
     gene            14439..15677
                     /locus_tag="PLQ70_01280"
     CDS             14439..15677
                     /locus_tag="PLQ70_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HQE33277.1"
                     /translation="MKKITLPLILLFVAFSGFSQGNKVTQAAGKFDGLAMTPPMGWNS
                     WNTFETNIDEKLVKETADIMVASGMAAAGYNYIVLDDGWMTKERDANGNLVPDPIKFP
                     NGMKALVEYVHSKGLKFGLYNCAGTQTCAGYPGTRGFEYQDARFYASLEIDYLKYDWC
                     NTAGITAPEAYKTMSNALKTAGRPIVFSLCEWGDNQPWEWAKPMGNLWRISGDIYPCF
                     DCEFHHEEGNWSSWGFMKIAEMRKDIRKYSGPDHWNDFDMMEVGDGMTNTEDKTHFSM
                     WCMLASPLIAGNDFRKMSPETLAILTNKNLIAVNQDKLGIQGFKLTVEDGVAVWVKPL
                     SDGAWAITFLNRTELPKKVNFDWKKNPIKDADFGFEADFNKTIFKIKDLWKNKDAGTT
                     KKNLTADIASHDVITLKLIP"
     gene            complement(15788..16171)
                     /locus_tag="PLQ70_01285"
     CDS             complement(15788..16171)
                     /locus_tag="PLQ70_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQE33278.1"
                     /translation="MGFFSALIGNAGAVSQESLVKDYGKLLIESEEIELGFKLIRDVF
                     IFTNKRLILVEKQGITASKIEYKSITYKSISRFSVETAGTFDLEAELKIWVSSETHPS
                     IVKQFNKSVNLYDVHMVLATHVLNS"
     gene            16431..19094
                     /locus_tag="PLQ70_01290"
     CDS             16431..19094
                     /locus_tag="PLQ70_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HQE33279.1"
                     /translation="MSESGVETNAEKERLKLRADNKGWKKWGPYLTDRQWGTVREDYS
                     QSGYAWGSTTHDMARSKAYRWGEEGIGGISDNKQHICFAFSFWNHKDRILKERFFGLT
                     PAESNHGEDVKEIYYYQDSTPTHSYQKMLYKYPQKPFPYQKLIEESKKRSRLETEYEL
                     IDTGIFDNNEYFDITIEYAKADEQDILIKVTVENRSKEAAPITVLPTIWFRNTWSWGY
                     ENYKHKPLLKGIGKSLIEVYHHTVGSFNLFAEEAKDLLFCDNKTNYEKLYGSPAQSAY
                     TKDGINDYIINKKSDRVNPEAVGTKASVLYDDIIPGNGKKEYRLRFTNGNPIDVFEDF
                     DAIFQKRIDEADEFYAPIQKGVKDEKLKSIQRQAYAGMLWTKQWYYYNVFEWLKGDPS
                     KPKPENGRNEGRNSTWKHLYTSNILSMPDKWEYPWFAAWDLAFHTLPLARLDPDFAKR
                     QLSVILREYYMHPNGQIPAYEWSFSDVNPPVHAWATWKVYEIDKETNGGVGDTVFLER
                     IFHKLLLNFTWWVNLKDENGNNIFGGGFLGMDNIGVFDRSADLPTGGHLEQADGTGWM
                     AMYCLNMLRIACEISLKNPVYQDMASKFFEHFLHISGAMQELGDNHLSLWDEEDQFYY
                     DMLHKSNGDAELLKIRSMVGLIPLFAVEVLTPELLDKLPLFKRRVEWVLKNRPDLASL
                     VSSWYTPGEGETRLLSTLRGHRMKMILKRMFDEEEFLSDFGVRSLSKYHKENPYKFKH
                     DGGIIQVDYTPAEATGDMFGGNSNWRGPIWFPMNYLILDSLEKFHSYYGDDFKVSYPT
                     GSGNETTLKEAAEGVAQRLLALFIPDSKKNIPMYGEYEKFQKDPEFNKNNLFFEYFDG
                     DTGKGLGANHQTGWTGLIAEIIRHLHGEKKV"
     gene            complement(19171..20241)
                     /gene="arsB"
                     /locus_tag="PLQ70_01295"
     CDS             complement(19171..20241)
                     /gene="arsB"
                     /locus_tag="PLQ70_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HQE33280.1"
                     /translation="MSANNCAPVVERKKLSFLDSYLTLWIFLAMALGVLIGYFIPSSG
                     NFINSFSSGTTNIPLAIGLILMMYPPLAKVKYEQMGEVFKNTKVLGASLFLNWIVGPI
                     LMFFLALLLLNGYPEYMIGVILIGLARCIAMVVVWNDLADGNREYAAGLIALNSIFQV
                     LLYSVYAYVFITILPPYFGYTGFEVNISIGQIAESVGIYLGIPFALGIISRYALIAMK
                     GSEWFETKYVPFISPITLISLLFTIVLMFSLKGELIVQIPLDVLRIAVPLVIYFTIMF
                     VISFFIGKYFGADYSKSTAIAFTATGNNFELAIAVSIGVFGINSGQAFAGVIGPLVEV
                     PALIALVNVAFWFRKKYFYKAI"
     gene            complement(20243..20875)
                     /locus_tag="PLQ70_01300"
     CDS             complement(20243..20875)
                     /locus_tag="PLQ70_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013868653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HQE33281.1"
                     /translation="MTSTKTNLFPEIENVISTLNFESTSEERKIVLQPLIDFIQRKSD
                     EQKEIRLNLICTHNSRRSHLSQVWAQTAAAHYGIQNVFCYSGGTEATAMFPMAAKTLE
                     KQGFQIKTISEGNNPVYAIKYSENAHPIIGFSKTFDDDFNPQSEFAAILTCSSADQGC
                     PFIAGAEKRIPITFEDPKAFDNTPQQAEKYEERSVQIATEMFYVFSQIKN"
     gene            complement(20901..21371)
                     /locus_tag="PLQ70_01305"
     CDS             complement(20901..21371)
                     /locus_tag="PLQ70_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HQE33282.1"
                     /translation="MKLSEVKNILKSVEAVNFILTDGTMVPEHFHVTEVGLITKNFID
                     CGGTVRKENVVNFQLWDANDFEHRLKPQKLLNIIELSEKVLGIEDFEIEVEYQAQTIG
                     KYDLDFDGKNFVLLNKQTACLAQDQCGIPTEKQKVRLSDIKIEQSCCTPGGSCC"
     gene            complement(21389..21721)
                     /locus_tag="PLQ70_01310"
     CDS             complement(21389..21721)
                     /locus_tag="PLQ70_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQE33283.1"
                     /translation="MGASKTDSFTEKQNELAILAKALGHPARIAIIEYLLKVDACICG
                     DIVNELPLAQPTISQHLKELKNAGLIKGNFEGTSICYCIDELGFEKIKGFFQDITKYL
                     EKRKNNCC"
     gene            complement(21956..22366)
                     /locus_tag="PLQ70_01315"
     CDS             complement(21956..22366)
                     /locus_tag="PLQ70_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HQE33284.1"
                     /translation="MLSEENVYPTNEVLEIILGESYVVFNKFIEIITNKNIGLGVEWR
                     YYKDGKSWLCKVSLKKKTVFWLSVWDGYFKIGFYFAEKNSSEIENLDIANTIKEDFKV
                     SKKIGKLIPLAISMNRKEQITDVLKIIEYKKNLK"
     gene            complement(22511..23200)
                     /locus_tag="PLQ70_01320"
     CDS             complement(22511..23200)
                     /locus_tag="PLQ70_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4919 domain-containing protein"
                     /protein_id="HQE33285.1"
                     /translation="MKKIILLFTILILSVNASFSQENSFKKPDYDLIKKNIEDKSSNY
                     YYPKLLERMISNDTLLDNEDYRHLYLGYVFNPKYSSFFKSSTEEKLRKYYQNEKINEK
                     EYDEIIKLGEQSIKEFPFDLRQMNYLAYIYHLKGDEISAKNISNRFHKIILAIMSTGD
                     GQKCETGLHVISVSHEYVILNLFKLQNISQSLVENCDYMAFEKGKYKVDGIYFSIEKI
                     LADEAKLFKGK"
     gene            complement(23241..>23804)
                     /locus_tag="PLQ70_01325"
     CDS             complement(23241..>23804)
                     /locus_tag="PLQ70_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008241364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQE33286.1"
                     /translation="AKKYNTQKTVPQEKIEELKAILRLSPSSINIQPWKFTFVQNPEI
                     KAKLASVSMHNTEKINQAQLLVVFSVADDLDAFENEVVNEFPQFRKEMFHKIKTYNSE
                     ADLKIWLAKQVYIALGVGLTASAAMGLDSTAMEGIETDKYKTILNMKDYKPLFAMAVG
                     YGAEEDANRLEVTPKSRRVLEDVVETI"
BASE COUNT         7831 a         3986 c         4174 g         7813 t
ORIGIN      
        1 aggattctaa aaatcgcttc tgggtgggtt gtatcaatgg gcttcagcaa ttcaaccgtc
       61 aaaccaatca gtttgacgac attaaacttt tgtcggcaaa caatcaactt ctgagtacgc
      121 atatcacttc cattatcgaa agtaaggatg gaaatatctg gataagcacc tccggagaag
      181 gcgttttgag aatgaccaaa gaccgggata aaacttttcg aagtgtaact tacctcaatt
      241 caaaacttct caatcaatat ttaactttca tttttcagga cagttccggg aaattctgga
      301 ttggcagtga aaactacggg ctgaatttgt acgatcccga taaggataaa atgtatgtat
      361 ttagtgttca gaacaataaa atcgggagta atcaaatttc ggccattaca gaggacagca
      421 aaaaaaatat ttttgttgga accctttccg gtggccttta taaattcaat aaaaatgatt
      481 tttcgtttat ttcgataaag gatgccaatg gaaatagcaa ccagccggtg aaaaccattt
      541 tcttagataa aaataatcgc ttacttgtgg gcactgatgg gcaagggctg aaaacataca
      601 attatcaaaa aaatctgctg gaagatatgg aaatcaaatc ggctcctttt gatttttcca
      661 aaacgaaagt gcatgccatt cttcaagaca gagctggaaa tttatggttc ggactttttc
      721 agaaaggcgt tttcctggtc agaaacaacc cttatgcgtt taattatatt gggcacaaat
      781 ctttcagtca gaatttaatt ggttccaata gcattgcttc cattcttcag gacagaaata
      841 atgaactttg ggtaggcact gataacgacg gaatgtacag gcttgaaaaa aacttcaaaa
      901 attctgttca ttacaatcct cctgccgttc ccaatattgt ttcttctatt gtggaacttg
      961 acaatggaga aatgtggctg ggttcttttt cgcaaggact ggccagtttt aataaaaata
     1021 ccgggaaaag cacctatttc aataatattc cttcctcttt tcagtacaac aatctggcaa
     1081 ttaaaatcaa ctgtatgata aaggatcaga atgataatct ctggattgga acacatggcg
     1141 gaggattatt tgtttttgac ccttctacgc gtaaatacat aaaacatttt ggcagcgatg
     1201 taatttcgaa tgactggatc agttctctat ttatggataa ggatcaaaga atttggatag
     1261 gaacttatga caatggaatt aatttagtag aagaaaatca aggtaaaatt agttttatac
     1321 acaactttaa gaattatcct tttgaagaat ctagtaaagt tagatgttta tctgaagggt
     1381 ataaaggtca aatttgggta gccactacaa acggattagt aacttttaat ccagattcgt
     1441 acaatcccaa tcaaataaaa ttttcaagat atgtaaaaag tgctaccgac aaatcgagct
     1501 tgaacagtaa cgatgtattg tttatatgca aagattccga agacaaaatc tggctttcta
     1561 cagcgggtgg cggccttaat ttggcaatgg aaacccaaaa caaccaatta aaatttaaaa
     1621 actttactaa agaaaatggc ttgcctagcg attttatctt gagtatggta gaagatgacg
     1681 agaagaattt atggttggcc actgaaaacg gtatttcaaa atttaatata aaacatcaaa
     1741 cttttagaaa ttacgatact tatgacggtt tgcttaaaac cagattttct gaggcatcta
     1801 gtttaaaatt acaaaatggc aatttgattt ttggttgtaa aattgggtat ttaaatttca
     1861 accctcaaac cattaaaaat caaaaaagta caactcaaat ggtttttacc aattttgaag
     1921 tcaataataa aatttcgaat gtaaaatcgg ctgaatttcc tttaaaacaa gatatcaatt
     1981 atgacaacaa tattgaattg gattaccaac acaataccat cagtatttat tacaccgttt
     2041 tagattatag atcgaataat aaacaacttt atgcctaccg tttaaaggga ttagaagata
     2101 cttggcatca ggtgaaaaac cagaaaaaag caacattcac caatttagat cctggcgatt
     2161 atgagtttga agtaaaatgt ttgaataccg atttatacac taatgtccct caaaaaaagc
     2221 tgtcttttac cattactcct ccgttttgga aaaccaattg ggcttatttt ttatacgcca
     2281 ttatctttct tgcattgcta gaaatgtcca gaagaattgt gtattcgatg attcaattgc
     2341 gaaataaagt agtggtagaa catcaaatga ccgagttgaa attgagtttc tttactaata
     2401 tttcacacga attgcgaact cctttaacac tcattgtaaa cccaattgaa gaaattgcta
     2461 aaaacgaaaa cttaagccct ttagggaacg aatacatcga aaccgtccga aaaaatgccg
     2521 atagattagt tcgttttgtt aatcaattac tcgattttag aaaagtacaa agtggcaacg
     2581 aagagcttca tatcgcacct attgaaatgg ttacttttat caatgaaacc ataactttgt
     2641 ttgtacatat tgcacacgaa aaaaatatta aaattcaaac aaaatctaat tttggaagca
     2701 taaatgccaa tctcgacaag gaaaaaatag atattgtaat ctataatttg ttgtctaatg
     2761 cccttaaatt ctctccaaat gatagcgtca ttacagtaga acttgcttta gaaaaagaca
     2821 attttttaaa aatatatgta gtcgatcaag gaacgggtgt ggatgaaaat aaattggaag
     2881 acattttcaa actttattat gaaaccaaca cgggtaaaaa taagaatgta ggaaccggaa
     2941 ttggattggc actttgcaaa gaatatgttc agttgcatca aggatctatt tatgctgtaa
     3001 ataatactga tggcggtctc accgttgcta taacaatcga tttaaacaat gaattgcttc
     3061 aaagtcattc gaatactata gcgtcggtag ctcaaaaaaa tcaaaaacaa cctcttgtta
     3121 ttgctcaaaa caatgaaaat gtagcagtcg aaaacgacac taatcttcca ttggttttaa
     3181 ttgtagaaga caacaatgaa ttgagaaaat ttttgaaaag tcagttgcaa tgtttttatc
     3241 gaataataga agccgaaaac ggaaaagtag ctttagaaat agccacaaaa aaactgcccg
     3301 atttgatttt gagtgatatt atgatgcccg aaatggatgg cattgagttg ttggatgcgc
     3361 ttaaaaattc agaggaaact agccatattc cagtggtttt attaacggca aaatcatctg
     3421 tcgaaagcaa aattgaaggc ttgaattatg gggctgatta ttatattacc aagcctttcg
     3481 acaccgattt tttattggct tctatagaaa atttaattaa gcagaggaag aaattttttg
     3541 aaaacttgca agcgaatgtc aaaacaatgg ctttagaacc aagtgaaata atcattacaa
     3601 ccaaggacga acaatttcta aaagatagca ttgcgattgt agaaaacaat ttgaccaatc
     3661 caaaattcaa cattgatgta atctccaaca gcttgagtat ttccagagtc acattcaaca
     3721 gaaaatttaa aagtttgacc aatatgactc ctgtagaatt tgttagagaa atgcgcatca
     3781 aaagagccaa acaatttcta gatgcaggcg aaaccgatat tgctggtatt gcttataaag
     3841 ttgggtttaa tgatgcgggg tattttagta cttgtttcaa agaatactac aaaattgctc
     3901 cttcggatta cttgaaacaa aaacaagaat aacattcaaa agtgtattat ttataagctt
     3961 tttgagtaga aaaatagtgc agttttaaaa ccttttttat ttgatttatt tcgattttaa
     4021 tgccaattta tttttacatt tgactgatta agttttgaaa ttaaagaatc taaattatgt
     4081 gggaacaagt acaagaaacg gttagtttta taaaagaaaa aaccaatttc aatccagaat
     4141 atggtgtgat tttgggttca ggattgggga gttttacaga tgatattgca atagaattta
     4201 ctttgcctta tggcgaaatt ccaaattttc cagtttcgac agttcatgga cacaaaggag
     4261 ctttggtttt tggaactatt ggcaacaaaa aagtggttgc tatgcaaggt cgttttcatt
     4321 tttatgaagg ctattcgatg aaagaagtta cttttccggt tcgtgtaatg aaatatttag
     4381 gagtcgaaaa attaattgtt tctaatgctt cgggaggtgt aaatcctaat tacgaagtgg
     4441 ggtctattgt aattttaaaa gatcaaatca atatgatgcc ggaacatcct ttgcgtggta
     4501 aaaatgacga acgatttggc cctcgttttt taaatatgag tgaacctttt tctattaata
     4561 tgattgctgt ggctaaagca ttagctaaaa atcttaatat tacagttcac gatggaatct
     4621 atcttggact tcaagggcca acatttgaaa cgcttgccga atataaaatg gtcaaaattc
     4681 ttggagcaga ttgtgtagga atgtccactg ttcctgagat aattgtggct cgtcatatgg
     4741 atatggagtg ttttggacta tctgtaatta ctgatatggg caatgaggaa aatatagaaa
     4801 ctgtttcgca cgatgaggtt cttgaagcgg ctaaaaaagc cgaacctaac gtgagaagtt
     4861 tgattaaaga attgatttta aattattaga agcttaaata tattttgcaa tagcaatata
     4921 gaaatcttca ggtacaaagg gttttgtaat gacatcattc attccgtagg acaaaagcat
     4981 ttctctgttt tcattcaacg aaattgctgt gagtgctatt acaggagttt tggtgtcaaa
     5041 ttttcttatt tcttgggtgg caattgttcc atttattcca ggaagatgaa cgtccattaa
     5101 aattaaatca tattggtttg ttttgcaggc ttctatcgct tcttctccat tgtcaattat
     5161 ctcacattca atgcctttgt tgttcagcat ttttttggta atcatttgat taattttatt
     5221 gtcttcgact accaatattt ttttgtttac aaaggctata gtgtcaattg tagtttcttt
     5281 tttgtcagta tcttgaaccc ttttagaaat tttgaatggt aattcgaaac taaatgtgga
     5341 gccttcattt actttacttt caagatttat ttttccacct aataatcgaa ctagtttcct
     5401 tacaatagtt agtcccagac ctgttcctcc gtattttcgg ttaatatcta ttgatccttg
     5461 cgaaaaactg tcgaacacac tttcgaattt atcttcagga attccaatac cattgtcttt
     5521 cacttcaaag taaacaaatg cttttttatc ccgaacagaa actaattttg tggtcacagt
     5581 tacctctcca tcatgagtaa attttaaggc attattaatc aaattcatta aaatctgaga
     5641 taattttgtg gggtcaccaa ttaaataatt ggggatttca caatcgatat tgaaattaaa
     5701 gctattgtcg ttttcgacag ctaactcttt caatgagctt tgtatatttt caactaattg
     5761 tttaaggtta aaattgatat gctcgatttg tattttactc gagtcaattt tgttgatttc
     5821 taaaatatca ttgataaatt tagttagata attccctgaa tactgcaaag attctaaata
     5881 ttgcatttga gatttcttag gattctcttc taataaaagg tgagtaatac cgttaatggc
     5941 atttaaaggt gttctaagtt catgacttac agtagataag aattcagatc tggcattgga
     6001 tgctttttct actttttctt tagcaataat gagttcttta tttttctctt ctaataattt
     6061 attagattga gttctaatga tattattttt gtataatgac aaactcaata atgacaaaat
     6121 cgatattaat gcaatggcaa gaatgctaat cagtttggaa aacttagatg ccttttcttg
     6181 ctgtttggct tcttcattta tttgttttat ttcttttaaa cgttccgatt ccttgaagtt
     6241 ttcataatca tccactccaa gtttttcatt gtctataatt gatatacttt cttttaaatt
     6301 ggtgtgctgt ttcaaataag agtaagcact atttcgatcc agcatttttt cataaaccgt
     6361 acttaaggca agtaaaatag ctgatttttg ttctagattt ttgcttttag aatttaatgc
     6421 caaagcttta ttgaaataag tcaatgccaa attatttcta ttttgagcca tttctatagt
     6481 tccaatttga taaagagcct cagatttgct atctaaaata gatggactat caggtttgac
     6541 gattatttga ttaaaaagtg tagaagccaa atcatatttc ttatctgatt tgtaaatgat
     6601 acctcgctga aggttaatta aatcagcatg atcaataatt tctagggtat tgtatagtga
     6661 ttcggcttgg tcaaaattat atttggcatt ggtataactt ctttttttca taaagcacat
     6721 tcctaagtaa taataggctg ttgcttgcaa agaatttgga gtatttgttt tagataatgt
     6781 aagactattg ttgaaatatt ttaacgcatc atcatatttt ttgacatcat aatacaattt
     6841 tcctaagctg aaattctcat ttattaaggc atcagtgttg tggttaattt tagaatactg
     6901 aattgctttt tgggtgtaat gaagtgcatc cttatattta tttgctttta catttacatc
     6961 acgcaaattg ttgtaatagg caatgctatc gatcttttca accggttttt tcttttgaga
     7021 aaatgaaaag atactaacga ataagaaaaa aaatagaatt gatttcatcc gacatttgag
     7081 atttccttat gaagtgggta aaggtattga attatttttt aagtagttaa aataattaaa
     7141 tcaataattc tggaagaata tccaatttcg ttgtcatacc aacccactac tttcaccatt
     7201 ttatcaataa ccgatgttaa ttgagcatca aacaggcaag aatttctgtt gccaataatg
     7261 tcaaccgaaa caatagggtc ttcggtataa gccaaaatac ctttcaaatt cgtttcagaa
     7321 gcaagtttga aagcggcatt gatttcctcg atgcttacgg cacgttttac gttgaatgta
     7381 atatcagtca acgagccatc tggaacggga acacgaatac cgcaaccccc aattttacct
     7441 tcaaattctg gaaaaatttt tgtcaaagcc ttggcagcac cagtagttgt tggaacaata
     7501 gattgacttg caccacgagc tcgacgtaaa tctttgtgcg gttgatcatg caaactttgg
     7561 tcagttgtat aagaatgaat ggtggtaatg taagcttgtt ctattccgca aagttcgttg
     7621 atgattttca tcattggagc ggcgttgttg gtcgtacagc tagcatttga aatgattgtt
     7681 tcagttccgt ccaaaatatg ttcgttgaca cccaaaacca ctgttttaat agtatcgacc
     7741 tctgaagggg cagaaagaat aactttttgg gctccagcca aaatatgagc gttaatttcg
     7801 tcgaaagttt tatattttcc agtcgattcc acaacaacat caatgttggc agatttccaa
     7861 tcgagcttag aaatgctttt ctcgtggaaa aaaagataat gttttccgtc cacgataatt
     7921 cctttttcat cagaagaaac aatcgatggc aaaaccccgt gaatgctgtc atatttgatt
     7981 aaatgcgcca ttgttttttt gtcggcaatg tcattaatgg caacaacttc tagggtagga
     8041 tggttcaaaa gcaagcgaaa taaatttcgt ccaattcttc caaaaccatt tatagcaatt
     8101 cttgttttca atttttttag cttaattgtt ttaactggtt aatcgtttaa ccgttgaaac
     8161 gattaatcag ttaacttttt tataatatat gtttctgagc tttatacgaa gaacgcacca
     8221 atgggccact ttctacgtga cggaatccta attctaatcc gaaagcttcg tatttagcaa
     8281 attgttctgg agttatgaat tcttttacgg gcaaatgttt tttgctgggt tgtaagtatt
     8341 ggccaatggt tacaatgtca actttggctg catataattc gcgcatggtt tcaaaaactt
     8401 cttcttcggt ttcgccaaga cctaacatga ttcctgattt ggttctgcga attccttttt
     8461 ctttcaagta tttcaatact tccaagcttc ggtcgtattt ggcttgaatg cgtacctcgc
     8521 gagtcaatct tctaacggtt tccatattat gcgaaactac ttctggattg gcttccacaa
     8581 ttctatcaat atttctttcg atgccttgaa aatcaggaat taaagtttca agagtggtgt
     8641 tggggttcat cctgcgaata gcttttacgg tttctatcca aataatagaa ccgccatctt
     8701 tcaaatcgtc tctatctacg cttgtaatca cggcatgttt gatgttcatt atttttatag
     8761 aacgcgctac tttttcaggt tcatcccaat ctacggtttc tggtcttccg gtttttacgc
     8821 cacaaaatcc acaagaacgg gtacaaatat ttcctaaaat cataaaagtg gccgttcctt
     8881 cgccccaaca ttcgcccata ttcggacaac ttcccgaggt acaaatggtg ttgagtttgt
     8941 aattctcaac taatccacga agttctgtat atttttgacc gattgggagt tttactctca
     9001 accattttgg ctttcctact ggtaatgtat tctctaaaac agtttccata tttatttttt
     9061 gaaagtgcaa agataaggaa aggaatttta gattttggat tttagatttt agatttcatt
     9121 ggaattaata taagattgtc agttttctgt tttttaattt aatgccccaa ttccataaac
     9181 ttttatatct aaaatccgaa ctctaaatta aaaaatcttt ctgctatctt tggcgtcctt
     9241 aaaaaaaaat aaattatgag cgcaatgtgg tatgaatgca aagtaaaata tagaaaaact
     9301 gacgaaattg gaaaccaaaa agtcacaacc gaaccctatt tggttgatgc tttatcctat
     9361 acagaagccg aatccagaat caacgaagaa atgaaagctt atattagtga agaatttaaa
     9421 atcacgaata taaaagtggc caattatgcc gaaattcatc ctttcgaaaa tgccgatcgt
     9481 tggtttaaat ccaaagtttc attaatggcc tatgacgaag aaagtggcaa agaacgcaaa
     9541 accaatatgt atgttttagt acaagccaac gatgtcaaag aagcttacga caacaccatc
     9601 gaagtaatga aaggaacaat gggcgactat accattccag ccatttctga atcgccaatt
     9661 gtggacgttt ttccttattt tagtggtgat gaagacgaat tagaacaact agaaaaattc
     9721 aacgcactca aagcttcgaa acctgaaaaa gctaccgaaa tagaagacca catggaattt
     9781 gtagatgaag aagaggaagt tgtttcctaa caaatttcat taaaaagagt atccacaaat
     9841 tcgcaaatta taaattacta ttgagtttat aatttgcgaa tttgcggttt tgtaattttc
     9901 aaagtctttt aggattctaa ctttacaatt aaggattaat aatgagtttg tgaacagatt
     9961 ttttgttccc agaatgaatt actactaaat aaactcctgc tttcaaataa tcacgattaa
    10021 tttttaattc ataatttcca gcatttttat aaccatcaaa aagagaaatc gcttcttttc
    10081 cttggatggt aaagagttta atggaaacgt tggatgattc ttctaattgg tatttcaaaa
    10141 gggtagaggt aaccattgga ttggttgaaa tctgacttgt caactggttt ttttctaggt
    10201 tttgaagtcc cagaaaatcc gttgttttca taaaacggga gggattaagc acttcatgag
    10261 taacttttac agaatgaatc gctcctttga accaagccac ttgattcatt cgaacaccca
    10321 atgaggtttg cccagaattt actacttgat aactaaccgc acctatcaat tcttgtatgc
    10381 catttacaaa acttgtcatt acgccatttt tataagtcaa agctatatgc acccaattat
    10441 tcatgctgtg cactaaggaa taatccaata aagtttggtt tgaagtccca gatttaataa
    10501 aagtatccag actccaattc aaattattat tgttacgcaa ctctgccaat attctattat
    10561 tgtcgtcttg ttgaaaatgg agatagcgtt gttcaactgc tccacctgaa tacggtttta
    10621 gaatcatttc aatggtaaat tcagtggctc cgttcatcgg attatcattt acaagaaggc
    10681 catctttgat tccgtcaaat tcaaccgcag tacccaattc tgtttgaatg accttgggac
    10741 ttccgcttac agtaacagat ttgccgccaa tttgagtaag attgtccaca cgccaaaaaa
    10801 ctgtttttat ctgagcgctc gaaatgtaga atgtgaggag cattataatt agcgaaactg
    10861 attttttcat ttactatttt tctttattac acctcaaatc acaacttcta aacattgtat
    10921 tggggtggtt tattgttgat taggatgtga atatagtaaa tttagttttt atattgaaaa
    10981 ttttgtttta tccaataata ggttaaagcc aaatagtaac atctatttct ttttccgctg
    11041 aaatattttc tttttttctt atttcagctt ttaatggcaa taagtatgtg ttaatttttg
    11101 tatcaaacca aatggctgtt ttccattcac ttttgccaat ttttgctaca gcttttaaac
    11161 gtccccaacc ttcttcttga aattttaaaa tacttcttat ttcttttgac atttcttttg
    11221 gaagagcaac aaaataccaa ccgcttggcg aagaatgttg ccaagatttt tctgaaaact
    11281 catattttat tttgccgttc aattaagatt ctgttttagt tattagtttt taaattagta
    11341 aaaacaaata tacggtttca attggaggta atttgcaaat actacaaagt agcttttgtt
    11401 gtagtgcaat atataaataa ttttatccaa gttttataaa tccccaaaat taacttcttg
    11461 taaagtttag attaaatttt gacaataaag tatcattgtt ttctgtcctt attctgtatt
    11521 attgtatgtt gcaccagtta attatttgca aatgaaaaat actcttaaaa caccatttct
    11581 actacttatt ttctttagtt tttgcctttc tgttttttca caatcaggtt caaaggagca
    11641 aaatagagat catatttccc tcgacaaaga ttggagattt gcttttggtc atccctccga
    11701 tacaaagaaa gattttaata ccggaattgc ttatttctcg tatttggcca aagcaggttt
    11761 tggtgatggc gctgccaatg ccaattttga tgatcgctcg tggcgaaaat tagacctgcc
    11821 acacgattgg gcagtcgaac aaggttttag caaagacgcc agttttagtc acggtttcaa
    11881 agccattgga cgcaattttc cagacaaaag tattggttgg tatcgcaaga aaattaccat
    11941 tccacaaagc gatttgggac gccgaattca tcttgctttt gatggtgttt ttcgtaattc
    12001 aattgtttgg ataaacggtc attatttggg caatcaagat agcggttatt tgggcttcga
    12061 atacgatatt actgattata tcaattatgg tggcgaaaat acaatcgccg ttcgagtgga
    12121 tgccacgatg gaagaaggct ggttttatga aggtgccgga atctatcgcc acgtttggct
    12181 caataaaagc aatgaacttc atattcctca aaacggcact tttgtaaaaa ccaaaacaaa
    12241 agacaataca accacggtaa atgctttggt tacggttgca aatgatggaa aagcttcaaa
    12301 atcattccaa attattcaaa caattaagga tgcaactgga aaaaaaatca ccgaaaaaac
    12361 aatcaattcc tgttctttaa atccaagaga aaccaaagac tttacctgcg atttgacggt
    12421 tgaaaacgca acactttggt caatcgaaaa tccatatttg taccaaatga caacctcgat
    12481 ttttgaagat gaaaaattgg ttgacacttt tgaaactact ttcggaatcc gaaccattcg
    12541 gtttgatgct aacgaaggtt tttttctgaa tgaaaagcac atcaaaataa aagggacaaa
    12601 caatcatcaa gatcacgcag gagttggagc agcaatgcct gatgccttgc aggattttag
    12661 aatcaagacc ttaaaatcgt ttggtagtaa tgcctaccga tgttcgcata acccaccaac
    12721 tcctgaatta ttagaggctt gcgaccgctt gggaatgcta gtcattgacg aaaacaggtt
    12781 gatgggaagc acacaaaatc aacttaatga tgttaaaaaa atgatagaac gagatagaaa
    12841 ccatccgtcc atcattagtt ggagtgttgg aaacgaagaa tggggaattg aaaatgatat
    12901 tgttggagct cgaattgcaa gttcgatgca atcttatgta aaatctattg atgcaacaag
    12961 accagcaaca gcagccttta gcggtggaat tggcagtaac ggaatcacaa cggtaatgga
    13021 tttgttggga atcaattata ttgcgaataa aagtaccgac aaacaacatc aattattccc
    13081 caatcaatgt ctttggggaa ccgaagaagg aagtaccaat gccactcgtg gcgaatatta
    13141 tcgggataac caaaaacgta ttatgccggc ttatgacaaa gctccaagtg ctagttttat
    13201 tagcatagaa aacggttgga aacattataa ctcccgtccg tatttagccg gaatgtttgt
    13261 ttggacaggt tttgattatc ggggagaacc aacgccattc gactttccgt cggtgggttc
    13321 ttattttggg atggtcgatc aatgtgggtt ttacaaagac accgcttggt atttgaaatc
    13381 gtggtggcaa aaccaacccg ttttacacct tttgccgcat tggaattgga ctggcaaaga
    13441 aaatcaaagt atagaagttt gggcctacag caattgtgat gaggtagaac tttttttgaa
    13501 caaaaagagt ttaggcaaaa aaacaatgca aaaagacagc catttggaat ggaatgtcag
    13561 ttatagtccc ggaacccttg aagctattgg atataaaaac ggcaagaaaa tcattaccga
    13621 tgttgtgaaa actactggag cagcagcaat tctgaattta gaatcgaata aaaatagttt
    13681 agaagcatcg aaaaatgata ttgcactaat aacggttagt gctaaagaca aatcaggact
    13741 tcatgttcca attgccgata acgaagttag ttttactatt tctggtccag ccaaaattat
    13801 tggcgttggc aacggaaatc caacctcgct ggaagctgat aaatatctgg aaactataac
    13861 tgttttgaat atcgaaaatt tgaaagaaaa aatggttgcc gattttaatg tttcagacga
    13921 aaccaaggaa aatgtagcaa ccgattcttg gcaaaaagcc tttacagatg atagaaatgc
    13981 agcttttggc gaaaaagtaa aaaacatcgt ttacagaacc gagttcgaat tgcctgcaaa
    14041 ttacactgaa tcaacaatta atttctttta taatagtctt ggaaaacagc aatctatttt
    14101 tattaatggc aaaaaaatag cagatgccat tcccgaaaac aagcatggcg acacatttct
    14161 attagataaa acgatgttgc attcaggcaa aaacaccctt gcaattgttg ccacaccatt
    14221 attgaaagta aacagttggg atgtggttaa tcaaaatccg ggaacgattc aattgattac
    14281 caaagcggat aattacaaac gaaaattatt caatggtttg gctcaagtca ttattcaaac
    14341 tactggcgaa gcaggagaag tgaaactttc ggctacagcc aatggattaa aatcaacaga
    14401 aataatcatt aaaacagtac aataaaaaac atttaaaaat gaaaaaaata acactgcctc
    14461 ttattctttt gtttgtagca ttttcgggct ttagccaagg aaataaagta actcaagccg
    14521 ctggaaaatt tgacggtttg gcaatgacac caccaatggg ttggaactct tggaacactt
    14581 ttgaaaccaa catcgacgaa aaattagtta aagaaaccgc cgatattatg gtggcttcag
    14641 gaatggctgc tgcaggatac aattatattg ttttagatga tggttggatg acaaaagagc
    14701 gtgatgccaa cggaaattta gttccagatc ccataaaatt tcccaacgga atgaaagctt
    14761 tagttgaata tgtacattcc aaaggattga aatttggctt gtacaattgt gccggtactc
    14821 aaacttgtgc gggctatcct ggaactcgtg gatttgaata ccaagacgct cgtttttatg
    14881 ccagcttaga gattgattat ctcaaatatg attggtgtaa tacggcagga atcacagctc
    14941 cagaagctta taaaacaatg agtaatgcac taaaaactgc tgggcgacct attgttttta
    15001 gcttgtgcga atggggtgat aatcaacctt gggaatgggc aaaaccgatg gggaatcttt
    15061 ggagaatttc gggcgatatt tatccttgtt ttgattgcga atttcaccac gaagaaggca
    15121 attggtcgtc ttggggattt atgaaaattg ccgaaatgcg caaagacatc cgtaaatatt
    15181 cgggtccaga tcattggaac gattttgata tgatggaagt gggtgacgga atgaccaata
    15241 ccgaggataa aacccatttt agtatgtggt gtatgcttgc ttcgccactt attgcaggaa
    15301 atgattttag aaaaatgagt cctgaaacat tggctatttt aaccaataaa aatctgattg
    15361 ccgtaaacca agacaaacta ggaattcaag gcttcaaatt aacggttgaa gatggagtag
    15421 cagtatgggt aaaacccttg tccgatggag cttgggcaat cacgtttttg aatagaacag
    15481 aattgcctaa aaaagtcaat ttcgattgga agaaaaaccc aatcaaggat gccgattttg
    15541 gttttgaagc cgatttcaat aaaactattt tcaaaatcaa agacctttgg aaaaacaaag
    15601 acgcaggaac aaccaaaaag aatttaactg ccgatattgc ttctcacgat gtcattacgt
    15661 tgaaattaat tccataatca aagaaatgaa aaagtatcga aaagccaaat tcttaaaggc
    15721 ttctcgatac ttttttgttc aaaatcccgc tccaaatgac ttaaaatgat tggaaagcaa
    15781 gtgtttttta tgaatttaaa acatgcgtag ccaaaaccat atgaacgtca tatagattga
    15841 ccgatttgtt aaattgtttt acaatacttg gatgagtttc gctcgaaacc caaattttta
    15901 gttcggcttc caaatcaaaa gtacctgctg tttcaacgct aaatcttgag atactcttgt
    15961 aagtaatgga tttgtattca attttgctgg ctgttatgcc ctgtttttct accaaaatca
    16021 aacgtttatt ggtaaaaata aacacatcgc gaatgagttt gaaacccaat tcaatttctt
    16081 cactttcgat taataatttg ccataatctt ttactaaact ttcctgactt actgctcctg
    16141 cattgcctat taaggccgaa aaaaatccca taattttgtt gttataagtt aaatattcta
    16201 aactgctcct gcaaaattac cataaaaaaa ggtttagggc ctcaagaaat actacttttt
    16261 gaaaaaagaa tagagcaaaa ctttgacttt ctagcaattc tacaaataca ataataaaac
    16321 aatttataaa tagtttttca gtaattaaat caattgattt ttaccaattc tataggtgtt
    16381 tttagactcg ttttatataa attgcgattc agaatcaaaa cagaatgagt atgtcagaaa
    16441 gtggagttga aaccaatgca gaaaaagaac gtttaaaact aagagccgat aataaaggtt
    16501 ggaaaaaatg ggggccttat ttaaccgatc gtcaatgggg aactgttaga gaagattatt
    16561 ctcaaagtgg ttatgcctgg ggaagtacca ctcacgatat ggcacgttct aaagcttatc
    16621 gttggggtga agaaggtatt ggaggtattt cagataataa gcaacacatt tgttttgcct
    16681 tttcattttg gaatcacaaa gaccgtattc taaaagaacg cttttttgga ttgacgccag
    16741 ctgaatccaa tcatggagag gatgtaaaag aaatttatta ttatcaagat tccacgccaa
    16801 cgcattccta tcaaaagatg ctttataagt atccgcagaa accttttccg taccaaaaat
    16861 taatcgaaga aagcaaaaaa aggtctcgtc tagaaaccga atatgaatta attgatactg
    16921 gaatttttga caataatgaa tattttgata ttactatcga atatgccaaa gccgatgaac
    16981 aggatatttt aataaaagta acagtagaaa ataggtcaaa agaagcagct ccaatcacgg
    17041 ttttgcctac catttggttt agaaatactt ggagttgggg atacgaaaat tacaaacaca
    17101 aacctctttt aaaaggaatt ggaaaatcat taattgaagt gtatcaccac acagttggta
    17161 gttttaactt gtttgccgaa gaggctaaag acctattatt ttgtgataat aaaaccaatt
    17221 acgagaaatt atatggatcg ccagcccaat cagcttatac taaagatggt ataaatgatt
    17281 atataattaa taaaaaaagc gatagagtaa atccagaagc tgtcggaacc aaagcatctg
    17341 tactttatga tgatatcatt ccaggaaatg gaaaaaaaga ataccgactt cgtttcacaa
    17401 atggcaatcc tattgatgtt tttgaagatt ttgatgcaat ttttcaaaaa agaattgacg
    17461 aagctgacga attttatgct cctattcaaa aaggagtgaa agacgaaaaa ttaaaatcga
    17521 ttcagcgtca ggcttatgct ggaatgcttt ggaccaaaca atggtattat tacaatgttt
    17581 ttgaatggct aaaaggagat ccatctaaac caaaaccaga aaatggacga aacgaaggaa
    17641 gaaatagtac ttggaaacat ctctatacat ccaatattct atcgatgccc gacaaatggg
    17701 agtatccttg gtttgctgct tgggatttgg catttcatac tttgcctttg gcaagattgg
    17761 atcctgattt tgccaaaaga caattatcag ttattctgcg ggaatattat atgcatccta
    17821 acgggcaaat tccagcttat gaatggtcgt tttctgatgt gaatccgcct gttcatgcgt
    17881 gggcaacttg gaaagtgtat gaaattgaca aagaaaccaa tgggggagtt ggcgatacgg
    17941 tatttttgga acgtattttt cacaaattgc tactcaattt tacttggtgg gtgaatttga
    18001 aagacgaaaa tggaaataac atttttggag gtggattcct cggaatggat aatattggag
    18061 tttttgatcg ttcagccgac ttgcctacag gaggtcattt agaacaagcc gatggaacag
    18121 gttggatggc aatgtattgc ttgaatatgt tgcgtattgc ttgcgaaata tcattaaaaa
    18181 accctgttta tcaggatatg gcttccaaat tctttgaaca ttttctgcat atttctggag
    18241 cgatgcaaga attgggagac aatcatctaa gtctttggga cgaagaagat cagttttatt
    18301 acgatatgct ccataagagt aacggtgatg ccgagttgtt aaaaattcgt tctatggtgg
    18361 gtttaattcc actttttgct gtcgaagtat taacacccga attgctcgat aaattgcctt
    18421 tattcaaacg tcgtgtggaa tgggttttaa aaaaccgccc agacttggct agtttagtat
    18481 caagttggta caccccgggt gaaggcgaaa cacgattatt atcgactcta agaggacatc
    18541 gaatgaaaat gattttaaaa cgtatgtttg atgaagaaga atttttatct gattttggtg
    18601 ttcgttcttt gtccaaatac cacaaagaga atccttataa attcaaacat gatggtggaa
    18661 ttatccaagt agattacacc ccagcagaag ccacaggcga tatgtttggt ggaaattcca
    18721 attggagagg tcccatttgg tttccaatga attacttaat tttagactca ttagaaaaat
    18781 tccacagcta ttatggcgat gatttcaaag tgagttatcc tacaggatca ggaaatgaaa
    18841 cgactctaaa agaagctgcc gaaggagttg ctcaaagatt attggcgctc tttatcccag
    18901 attctaaaaa aaatattcca atgtatggcg aatacgagaa attccaaaaa gatccagaat
    18961 tcaataaaaa caatttgttt tttgaatatt ttgatgggga tacaggaaaa ggcttgggag
    19021 ccaatcatca aacaggatgg actggactta tcgctgaaat tataagacat ttgcacgggg
    19081 agaaaaaagt ataacacctg tgttttaatg aaaaggctct tttaaaatcg aatgttttaa
    19141 aagagccttt tttctatata attttcaaaa ttatattgct ttataaaaat actttttcct
    19201 aaaccaaaac gcaacgttca ccaaagctat caaagctggc acttctacca aaggcccaat
    19261 tactccagca aaagcctgac ctgaattaat accaaaaact ccaatagaaa cagcaattgc
    19321 caattcgaaa ttgtttcctg ttgcggtaaa agcaatcgct gttgatttcg aataatcggc
    19381 accaaaatat ttcccgatga agaaactgat gacaaacatt atcgtgaaat aaatcaccaa
    19441 aggaacggca attctcaaca catctaacgg aatttgaaca atcaattcgc ctttcaaact
    19501 gaacattaaa acaattgtaa acagtaatga aatcaatgta attggagaaa taaaaggcac
    19561 atatttagtt tcaaaccatt ctgatccttt cattgcgatc aaggcatacc gactgatgat
    19621 tcctaatgca aaaggaattc ccaaataaat gcccacactt tcggcaattt gtccaatact
    19681 gatgttgact tcaaaaccag tataaccaaa ataaggaggc aaaatcgtga tgaaaacata
    19741 agcataaacg ctatacaaca acacttgaaa aatactgttc aaagcgatta aacctgcagc
    19801 atattcgcga tttccatcgg ctaaatcatt ccaaactacc accatcgcaa tacagcgcgc
    19861 taaaccaatt agaattaccc caatcatata ttcgggataa ccatttagta ataataatgc
    19921 taaaaaaaac atcaaaatgg gaccaacaat ccagtttagg aataaggaag cgccaagaac
    19981 tttagtgttt ttgaaaactt ctcccatttg ttcgtatttc acttttgcca agggtggata
    20041 catcattaat atcaagccaa tggctaacgg aatattagtt gttccgcttg aaaaggaatt
    20101 gataaaatta ccactcgaag gaatgaaata tccaatcaaa actcccaaag ccattgccaa
    20161 gaaaatccaa agggtaaggt aactgtcgag aaagcttaat ttctttcttt ccacaactgg
    20221 tgcacaatta tttgctgaca tattaatttt ttatttggga gaaaacatag aacatttcgg
    20281 tggcaatttg cacacttctt tcctcatatt tttcggcttg ttgtggggtg ttgtcaaagg
    20341 cttttgggtc ttcaaaagta ataggaattc gtttttcggc acctgcaata aacggacaac
    20401 cttgatcggc tgaagaacaa gttagaattg ctgcaaattc cgattgagga ttaaaatcat
    20461 catcaaaagt tttcgaaaaa ccaataattg gatgcgcatt ctccgaatat tttatcgcat
    20521 aaactgggtt gtttccttcg gaaatggtct tgatttgaaa gccttgtttt tccaatgttt
    20581 tggctgccat tggaaacata gccgtagctt ctgttccacc cgaatagcaa aacacatttt
    20641 gaattccata atgcgctgcc gcagtttgcg cccaaacttg tgataagtga cttcgtctgg
    20701 aattgtgcgt acatatcaga tttaagcgaa tttctttttg ttcgtctgat tttctttgaa
    20761 taaaatcgat aagtggttgt aaaacaattt tacgttcctc agaagtactt tcgaagttta
    20821 aagtgctgat tacattttca atttctggaa ataggttggt tttagttgag gtcatttata
    20881 ttggttttag tatttaaaaa ttaacaacag cttccgcctg gagtgcagca actttgttcg
    20941 attttaatat ccgacaatct cactttttgt ttttcggtag gaatgccaca ttggtcttgc
    21001 gccaaacaag cggtttgttt gtttaataaa acgaaatttt ttccatcgaa atccaaatcg
    21061 tatttgccga tagtttgtgc ctgatattcc acttcaattt caaaatcttc gattcccaaa
    21121 accttttcgg acaattcgat gatgttcaat aatttttgtg gtttcaaacg gtgttcaaaa
    21181 tcattagcat cccacaattg aaagttgacc acgttttctt tgcgaaccgt tccgccacaa
    21241 tcaatgaagt ttttggtaat taaacctact tcagttacgt gaaaatgttc tggaaccatt
    21301 gttccatctg tcaaaatgaa gtttaccgct tctacggact tcaaaatgtt ttttacttct
    21361 gatagtttca ttttatctga atttataatt agcaacaatt gttttttctt ttctccaaat
    21421 actttgtaat atcttggaaa aagcctttta ttttttcgaa tcccagttcg tcaatacaat
    21481 agcaaataga agttccttcg aagtttcctt tgattaaacc tgcgtttttg agttctttta
    21541 aatgctgcga aatagttggt tgtgccaagg gcaattcgtt tacaatatcg ccacaaatac
    21601 aagcatctac tttcaataaa tactctataa tggcaattcg tgctggatgt cccaaggctt
    21661 tggctagaat ggctagttcg ttttgttttt ctgtaaaact atcggtttta gaagctccca
    21721 ttattatatt tttatattgc aatattacga ttaataaaaa ttctagacaa aataatttta
    21781 aaaaaaatga ttttgagcac caagagaaca aaaaaaagtg gttttcaatt tcttttgaaa
    21841 accacttaat aatttaggac aactctataa tttaggattt gaaatcagtt ttgttgcctt
    21901 tgaatgtcaa ctgaatcctt tgctttgttg tgtttcattt tttgtactca ataaattatt
    21961 ttagattctt tttgtattca attattttta aaacatcagt aatttgttct tttctgttca
    22021 tagaaattgc taaaggtatc aatttaccta tttttttact gactttaaaa tcttctttta
    22081 tagtgtttgc tatatctaaa ttttcaattt ctgaactatt tttttcagca aaatagaacc
    22141 cgattttaaa atagccatcc caaacagata accaaaaaac agtctttttt tttaatgata
    22201 ctttgcacaa ccaagatttt ccgtctttat aatatctcca ttctacccct aaaccaatgt
    22261 ttttattggt aataatttct atgaatttat taaatacaac atagctttct cctaatataa
    22321 tttctaaaac ttcatttgta ggatatacat tttcttcgct taacaatcgt atttccataa
    22381 tttatactat ttagtttatt cttttttagc atacaaagtc agtttgaaaa aacggccatt
    22441 ttaagtagta atcgtctttt taatttttaa aattcttgcc aattcgattc taatgcaact
    22501 gctgacggtt ttattttcct ttaaaaagtt tcgcttcatc tgctaatatc ttttcgatgc
    22561 taaaatagat accatctact ttgtattttc ctttttcaaa agccatataa tcgcaatttt
    22621 caactagact ttgcgaaatg ttttgaagtt taaatagatt taggatcaca tattcgtggc
    22681 taactgaaat gacatgaagt ccagtttcgc acttttgacc gtcaccagtt gacataatgg
    22741 ctaaaataat tttatgaaat ctattggata tattttttgc tgaaatttca tctcctttta
    22801 agtgataaat gtaggctaga taattcattt gtcgcaaatc aaaaggaaat tctttaatgg
    22861 attgttcgcc caattttata atttcatcat actctttttc attaatcttt tcgttttgat
    22921 aatattttct aagcttttct tcggtcgaag atttaaaaaa cgaactgtac tttgggttaa
    22981 aaacatagcc taaataaagg tgtctataat cttcgttatc taatagcgta tcattagaaa
    23041 tcattctttc taataatttg ggataataat aattagagga tttgtcttct atattttttt
    23101 taatcagatc ataatcaggt tttttaaagc tattttcttg agaaaagcta gcattaaccg
    23161 acagtattaa aatggtaaac aacaaaatta tttttttcat gtttttatta tttttgacag
    23221 tagtgttaaa atgaattggt ttaaatagtt tcaactacat cctccagtac tcttcttgat
    23281 tttggagtta cctcaagtcg gttggcatct tcttccgctc cataacctac agccatagca
    23341 aataagggtt tgtaatcctt catattgagg attgttttgt atttatctgt ttctattcct
    23401 tccattgcgg tcgaatccag acccattgca gcactggcgg tcaaacccac tcccaaggca
    23461 atatagactt gcttggccaa ccatattttc aaatcggctt cagaattata ggtttttatt
    23521 ttatggaaca tttcttttct gaattgagga aattcgttga ctacctcatt ctcgaaagca
    23581 tccaaatcat cggcaacgct aaatacaacc aacagttgcg cttgatttat tttctctgta
    23641 ttgtgcatcg aaacggaagc tagttttgcc tttatttccg gattttgcac aaaagtaaac
    23701 ttccaaggct ggatatttat cgaagaaggt gagagtcgca aaatggcttt caattcctct
    23761 attttttctt gtggtacagt tttttgagtg ttgtatttct ttgc
//
LOCUS       DAOSNN010000012        21661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_2414_length_21661_cov_4.906045, whole genome shotgun sequence.
ACCESSION   DAOSNN010000012 DAOSNN010000000
VERSION     DAOSNN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 21661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21661
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2414_length_21661_cov_4.906045"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLQ70_01330"
     CDS             <1..425
                     /locus_tag="PLQ70_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQE33287.1"
                     /translation="APLGPVYQAGTLSGNPLAMAAGLAMLQALDSDREIYKRLEEKTA
                     YLAAGIDKVLKTNKVDFTINRVGSMISVHFAETPVVDFQSAAKGDNETFKKFFHGLLK
                     EGIYIAPSAYETWFITDALTYEDLDFTIQAIDKVSKTF"
     gene            complement(585..902)
                     /locus_tag="PLQ70_01335"
     CDS             complement(585..902)
                     /locus_tag="PLQ70_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33288.1"
                     /translation="MKKLIIAALLCIGFTSFAQEKKKNPNQGQFQTSSPQQKNENRVK
                     RMVEELNLDSSQEQQLVSLFKNYPTNGTNKLTPEERKEYNKKLKEILTPDQMALLKSK
                     RKQ"
     gene            complement(1031..1549)
                     /locus_tag="PLQ70_01340"
     CDS             complement(1031..1549)
                     /locus_tag="PLQ70_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33289.1"
                     /translation="MKTNAQELSENILKIVNKNLLVLALLLVGTTMLAQPKNGRPQRN
                     QMEQFTPEQQSQLIIKKMTLELDLNDSQQKEISAIISEKMTKKEAHKAEMKANKEKGI
                     KPSNDERFAMKMKMLDEQIATKKRMEKILNAKQFEKWTSMKEDFKENRQERFKENRHG
                     NQQERNQGKRQG"
     gene            1737..2210
                     /locus_tag="PLQ70_01345"
     CDS             1737..2210
                     /locus_tag="PLQ70_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQE33290.1"
                     /translation="MYKLSLFIVALALFSSCGKKENATEDFSTGGAATEKTTDPSTYD
                     PNRGLGKYSTVELGAQLDAAMAGEGEKIQSVKCSACHKLTDEKLVGPGWKGVTSRNKP
                     EWIMNFITNPDPMIDKDPKVQAMLELCMVRMPNQSLSDTDARNVLEFMRKNDGVK"
     gene            2244..4220
                     /gene="nosZ"
                     /locus_tag="PLQ70_01350"
     CDS             2244..4220
                     /gene="nosZ"
                     /locus_tag="PLQ70_01350"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HQE33291.1"
                     /translation="MKNKFLKATLAITMVAALFSSCKPKNSGDVVSGDAAQKAYVAPG
                     KYDEFYNFVSGGFSGQVSVYGLPSGRLLRVVPVFSVDPQSGYGFSEETKPMLNTSHGF
                     VPWDDQHHTELSQTNGEVDGRWLFANANNTPRIARLDLKTFKTAEIIELPNSAGNHSS
                     PFITENTEYVVAGTRFSVPSDNANGDVPINTYKKNFKGYLSFVKVGKEGQMDIAFQIE
                     APGVNFDLSHAGKGKSHGWFFFSCYNTEQANTLLEVNASQKDKDFIMAVNWKKAEEYI
                     KAGKGKKVSTKYAHNTWDEKTHTAKSEIRNEVLVLSAKELKDICYMIPCPKSPHGCDV
                     DPTGEYIVGSGKLAALIPVFSFDKLQNAIKNKQFDGEYDGIPVVKYEAALHGEVQKPG
                     LGPLHTEFDGKGNAYTTFFVSSEVVKWDIKSLKVLDRVPTYYSVGHLCIPGGDSRKPF
                     GKYLIAYNKITKDRYLPTGPELAQSAQIFDISGDKMQLILDFPTIGEPHYAQAAPADL
                     IRNNGQLKFFKIEDNKHPFATKGEKEAKVVRNGKRIDVYMTSIRSHFASDNIEGIKLG
                     DEVYFHVTNLEQDWDVPHGFAIKGADNAELLIMPGETCTLKWTPEKVGIFPFYCTDFC
                     SALQQEMQGYVRVSPAGSSVPLTFSLGTNLPATK"
     gene            4285..5304
                     /locus_tag="PLQ70_01355"
     CDS             4285..5304
                     /locus_tag="PLQ70_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HQE33292.1"
                     /translation="MNTSKITVFSKVFLLAVSALFFASLLFPMWRIELEAPQYPEGLV
                     LQLHANKIGGDVDIINGLNHYIGMKTLHTEDFLEFQIIPYIVGFFGLFALAMVFVNKR
                     KGVVLLFLSFLLFGVLAGVDFYRWNYEYGHNLNPNAAIVVPGMAYQPPLIGYKQLLNF
                     GAYSIPDIGGWLLIAAGILLFIIVAKETRLISKIIRKPKINPVLVLLLSFSFMSCGDF
                     KEDPIKLNKDNCDFCKMSIADGKYAAEIITQKGRIYKFDDIGCMVNYCKENTNTKIGA
                     YFVSNFAQDNVLIPAETAFFLSGGTIQSPMHGGIIAFSFEKEAKEFATKSNAKPMNWA
                     AIIKK"
     gene            5304..6533
                     /locus_tag="PLQ70_01360"
     CDS             5304..6533
                     /locus_tag="PLQ70_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HQE33293.1"
                     /translation="MKKIFYILLLFSSILQAKVIEVGTNKPIKSIQKAIVLAKVGDTV
                     LVHKGHYKEGNIIINKRIVFIGKNYPILDGQKKYEVLSIKADGVIVRGFKVIKSGYAT
                     LEDPCGIKVYNKNNVVIQNNILDDNFFGIYIQNGANCIVKNNKVSAYGKEEQRIGNGI
                     HCWKSDRLQIISNTISGHRDGIYFEFVTNSTIKGNFSTKNIRYGLHFMFSNDDAYISN
                     VFKNNGAGVAVMFTRRVKMVNNHFEENWGDAAYGLFLKEISDSFIIGNKFAKNTSGIH
                     MEGTSRILVQKNSFEANGWGMKIQASCMDNEIKNNNFVGNTFDISTNGSLVLNTFNGN
                     YWDKYEGYDLDKNGVGDVPFHPLSLFSVITENNPSAMLLFRSFMITLLDKSEKILPSI
                     TPDNFIDKKPLMHSLPL"
     gene            6530..7252
                     /locus_tag="PLQ70_01365"
     CDS             6530..7252
                     /locus_tag="PLQ70_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE33294.1"
                     /translation="MIEIKNIFKKFGKLEVLNDVNLTFSKGECIALIGPNGCGKTTLI
                     KSILGMVIPTKGDILFDKKSILNKYKYRDQIGYMPQIGRYPDYMTVGQIIEMIKKIRN
                     SDEVLDEDLIKAFELEKIFDKQMRTLSGGTTQKVSAILAFLFNPQVLILDEPTAGLDP
                     LASEILKEKIIKEREKGKLILITSHLLSELDDMISQIIFMQDGKVHFHKSISDLLEST
                     NEQKISKAIAKILKSESHEQNH"
     gene            7236..8003
                     /locus_tag="PLQ70_01370"
     CDS             7236..8003
                     /locus_tag="PLQ70_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQE33295.1"
                     /translation="MNRIIKIIFLDILKNKIVLGYTLILALLSWSSFGLEDNSAKGLL
                     TILNVILFTVPLVSILFATIYLYNSSEFIELLLSQPIKRKKIWLSLFLGLSLSMVAAF
                     FIGAGIPLLINAPDSVGLMMVIVGCLISLVFVALAFLSSILTRDKAKGIGISIMSWLF
                     FALLFDGMVLFLLFQFSDYPIEKAMVAVTAFSPIDLARIQILLQLDVSAMMGYTGAIF
                     KDFFGTAIGLSVSFLLLCLWVIIPFWVSLEKFKNKDL"
     gene            8016..8387
                     /locus_tag="PLQ70_01375"
     CDS             8016..8387
                     /locus_tag="PLQ70_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HQE33296.1"
                     /translation="MKTDIKDRADIEQLVAIFYGKIKEDASISYFFNEVAKVNWDEHL
                     PKMCDFFENILFFSGNYEGNPMVAHEDLHKKSEVRGEHFNHWNALFDATVDELFEGKK
                     AEEIKQRSVNIAAAMMQKAHR"
     gene            complement(8594..9148)
                     /locus_tag="PLQ70_01380"
     CDS             complement(8594..9148)
                     /locus_tag="PLQ70_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HQE33297.1"
                     /translation="MKKLIYLSLATFMFAAFGCKQNSETTPETTTEPTETPGMGQSGV
                     KDETSAPNIVQVAVGSKDHTTLVAAVKAAGLVDALSNAGPFTVFAPTNAAFDKLPAGT
                     VEGLLKPEKKGDLENILGYHTYVGSLKTEYMQDGQEFDMVFGGKVKITKKGDKTFVNG
                     SEIVASIPTSNGIIHVIGDVLLPK"
     gene            complement(9218..10450)
                     /locus_tag="PLQ70_01385"
     CDS             complement(9218..10450)
                     /locus_tag="PLQ70_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33298.1"
                     /translation="MQLKTKFWLKISLLNLCIVATLGVLMRYKIGFEFPFFDQKHLQH
                     SHSHFAFSGWISHTLMTLMVYYLQTKTLNLQIIKYNKIIITNLICAYGMLICFIIQGY
                     GFFSITFSTASVLVSYVFCYQYFKDLKLIEDDLLVKNWLKAALFFNILSSLGTFYLAY
                     MMASKNVVQDLYLSSIYFYLHFQYNGWFFFASMGLFLGFLNLRKSEHPFYETFFKLFV
                     FACIPAYLLSILWLDLPFWLYVITIIAAIIQVFAWFKFLLVLLKSKINSLEQYSPLLR
                     YILVFVGFALSVKLILQLGSTVPIISQLAFGFRPIVIAYLHLVLLAIISLFLLFYIYS
                     NHLIILNKNIKYGILLFSTAVILNEIVLAIQGLAAFSYIPIPYVNEILFGIAIVLLIG
                     IGCVAYFSIKKAKNKPLL"
     gene            complement(10450..11043)
                     /locus_tag="PLQ70_01390"
     CDS             complement(10450..11043)
                     /locus_tag="PLQ70_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQE33299.1"
                     /translation="MQIDLDLLYSWGAVAKKYKKNEVIFEEGEAAHFYFQIMEGSVRM
                     FNSNEEGKEFTQGLFCDGDGFGEPPLFINETYPANAITIQDSTIIKLSKDKFLKILDE
                     YPNIQKDFLILFAQKIHTKSKTSKDIINQKPEFRITSFLNSYKRKSGNSTERLLIPFT
                     RQEIANYTGLRVETVIRVFSKMNEAKKVEIIHHKIYY"
     gene            11189..11563
                     /locus_tag="PLQ70_01395"
     CDS             11189..11563
                     /locus_tag="PLQ70_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33300.1"
                     /translation="MKKIIYLLALFILVKPVLPVIDYAVNYEYISKVLCVNKDKPKMH
                     CNGKCHLMKELAKASENDKPISSDKKTTSQESEILFFEKIKTFQLASICFDDCPKTNS
                     GYSDLYYFLNSDSVFRPPIFIS"
     gene            11647..12483
                     /locus_tag="PLQ70_01400"
     CDS             11647..12483
                     /locus_tag="PLQ70_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33301.1"
                     /translation="MKFLRILPLVLLTILASCSNNEEPAVDELYGLSKIKEITNTTHT
                     IELYKHMGGLEQGYNEIALKIKDNATNQYIKNATVTWTPVMHMAMMNHSCPKSPVTKV
                     STDGLVYEGYIVFQMAQNATEYWDLKIDYTINGTSYTMTSVIDVPASAKQRVTTFTGS
                     DGVRYIAAYVDPHHPKVGVNDMVAGVWKMQDMMTFPVVDNYKLKIDPRMPSMGNHSSP
                     NNVDLVQTVAGDLYNGKLSLTMTGYWKINLQLLNAAGTVLKGETITDAVPASSIYFEI
                     EF"
     gene            12545..14542
                     /locus_tag="PLQ70_01405"
     CDS             12545..14542
                     /locus_tag="PLQ70_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE33302.1"
                     /translation="MKNFIIIIGLCFLFPKILFAQEKETDTITPKDLTEVIVIGKKAQ
                     LYQKQAKPLATIDEYLQQSTKIDMIRRGSYAWEPMINNMPTERTLVTIDGMRIFGACT
                     DKMDPITSYVEVSNLSEATLKSGQQGACHGATIGGSVDLQRNRAGFSNLGWNAAVNTG
                     FETNSKQKIVGSAINYANNSFYIDTDFMFRDAENYTAGNGQEVLFSQFRKFNISSTSG
                     FSFDKNKLLEGSIIYDKATDVGYPALPMDVSLAEALITSLKFEYVPESSPIENWETKI
                     YYNTITHKMDDTKRPSVPIHMDMPGWSDTFGFYSKAKGTLRSHHFIADLNSFYNRSVA
                     EMTMYPADSNEKLMFMYTWPDVKTLYTALFLEDNIALNCHSSLKFSTSLGNHFNEVAS
                     ELGLESLRIFYPNMKASKNRILPSVSSNYSFDENGFSYGFGLAYGQRAPSVSEGYGFY
                     LFNSFDTYDYIGNPELKNENSLETSASMGYKTQKWSAKMSASYFHISNYIVGKPDASL
                     VPMTIGASGVKIYTALDYATIFNTDLNLEYQFSDFIQWKGQLVYSYGKDFENQNLPFI
                     SPLRYFSSLNFKKEKFSSSIAVQGNATQTQFSPFYGEDRTPSYAILNFDAGYSLDFEK
                     VKCGLKAGVENILDTYYSTFSDWNNIPRKGRNFFLNVSFSF"
     gene            complement(14614..16149)
                     /gene="nadB"
                     /locus_tag="PLQ70_01410"
     CDS             complement(14614..16149)
                     /gene="nadB"
                     /locus_tag="PLQ70_01410"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQE33303.1"
                     /translation="MIQTNYLIIGSGVAGLTFALKIAEQFPDKKVTIVTKANADESNT
                     KYAQGGIAIVTDKIVDSYEKHIEDTLICGDGLCDEEVVRMVITEGPKRLKELIEWGAK
                     FDKNAAGTLNLGKEGGHSENRVVHHKDQTGQEIQRAISYQVHQKENILVLDYHLAIDL
                     ITENNRCLGAYVLDEKTNEILTFKSDFTLLATGGIGHLYGHTTNPVIATGDGIAMAYR
                     ANAVIKEMEFIQFHPTALYDASTGTSFLISEAVRGFGAYLRTKNGHRFMPDYDSRGDL
                     ASRDIVSQCIEKELKKSGDDCVYLDCSHLDMDGFIKHFPMIYERCKSIGIDLSKDWIP
                     VVPAQHYLCGGIVVDKMGKTSVENLYACGECSRTGLHGANRLASNSLLEALVYSDKIF
                     NYLATTSFEKNQFEGKVTDWKLIEKPAINPDYVAKRKTELQLLMRQNAGIVRNDADLL
                     QAKEELTLWKTEIEEKINKYQINADLCELQNLITIGTLIVQQSIDQVENRGGFVKTDN
                     TNS"
     gene            complement(16149..17078)
                     /gene="nadA"
                     /locus_tag="PLQ70_01415"
     CDS             complement(16149..17078)
                     /gene="nadA"
                     /locus_tag="PLQ70_01415"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002665330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQE33304.1"
                     /translation="MKNLKERILELKKEKNAVILAHYYQEADIQDVADYVGDSLGLSQ
                     EATKVDADIILFAGVHFMAETAKILNPSKKVILPDLKAGCSLAESCPADTFEKFTKAH
                     PDHIVITYVNCSAEVKALTDIVVTSSNAVKIVKSIPKDKPIIFAPDKNLGKYVIEQTG
                     REMLLWDGSCVVHEAFSLDKLISLYKENPDAKIIAHPESETHILKTANYIGSTAGMIE
                     FVKKDPSQKFIVATEAGILHKMKQEVPHKILIPAPSNEDNTCACSECGYMKMNTLQKV
                     YDCLLNETPEISVPDEVMKKALIPIERMLELSK"
     gene            17316..19127
                     /locus_tag="PLQ70_01420"
     CDS             17316..19127
                     /locus_tag="PLQ70_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33305.1"
                     /translation="MTNKQLHTFHIPVMGLAFTIDSPIRVAKYGISSVIALADDELIE
                     KMRAFYSEKFDVPYQEITKKIHDYRAERITSYLNLVDKIVKEKFENFKTELSESKLAL
                     ENYIAMLPNKSEIKQGLEHFLEDGIALKENVLKYLEENLYPGDIDVNIMTKLDKDNFI
                     NNEQLPVEFNDAHAALRGFANSNLSSSIVLSAGMNPRLFAYFESFPCFFPDANNQLKK
                     KIILKVSDYRSAMIQGNFLAKKGLWVSEYRIESGLNCGGHAFATDGILLGPIMEEFKH
                     KKDQLVQSAHDIMVKALAQKGMHTPETPLELKITVQGGVGTSEEHDFMLDYYNVDAVG
                     WGTPFLLVPEATSVDVETRELLRNAKEDDLYLSHISPLGVPFNTVRGTSNEMFKQKRI
                     DDDKAGSSCPKKFLALSKEFGPEGICTSSKKYQDVKLDELNEIKETLTQEDYDKKKFD
                     ITEKACLCVGLANASYLENDIEIKGQAQGVIVCPGPNMAYFDKEVSLLEMVKHIYGNA
                     NVMTDLNRPNLFVKELKMYIDYLKNDIKNISSEINASQVKKLKAFKSNLLEGIAFYQN
                     FFANTHYFENSIKEIQNQLEFYTNELVGVEVPELVVA"
     gene            complement(19207..19638)
                     /locus_tag="PLQ70_01425"
     CDS             complement(19207..19638)
                     /locus_tag="PLQ70_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQE33306.1"
                     /translation="MFSKTCEYAIRASIFIAAQSYQDKRVTIKDIAENIYSPKSFTAK
                     ILQILAKKEIVHSIKGIGGGFEIPKENIKRINLAQIVIAIDGDSIFKSCGLSVNHCCD
                     QHPCPLNKKFKSIRNDLAKMLESTCLEELVLDVNSGESFLI"
     gene            complement(19668..20384)
                     /locus_tag="PLQ70_01430"
     CDS             complement(19668..20384)
                     /locus_tag="PLQ70_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HQE33307.1"
                     /translation="MNTNNELILPKVSVSTPIYSLTSFTLLDYPHKSACILWFAGCNM
                     RCLYCYNPEIVLGKGSLSFEEILKFLHTRKNLLDAVVFSGGECLLHKNIISFITEVKK
                     MGFLVKIDTNGSSPDIVQKLLNKGLLDYIALDYKAPKSKFKAITKSNLYDTFEKTFDL
                     ILQAKIPFEVRTTYHSELLLEEDLKEMIRFLESKNYSGNYYIQYFKNDVETLSKLERS
                     NNRIDAEKLSTNTIQIVLRE"
     gene            complement(20341..21627)
                     /locus_tag="PLQ70_01435"
                     /pseudo
     CDS             complement(20341..21627)
                     /locus_tag="PLQ70_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
BASE COUNT         7027 a         3500 c         3948 g         7186 t
ORIGIN      
        1 tggctccact tggtccagtg tatcaagcgg gaacattatc tggaaatccg ttggcaatgg
       61 cagcaggatt agctatgttg caagctttgg attcggatag agaaatttac aaaagattag
      121 aggaaaaaac agcttatttg gccgcaggaa ttgataaagt cctaaaaaca aataaagtag
      181 attttaccat caatagagta ggatcgatga tttcagtaca ttttgccgaa acgcctgttg
      241 tagattttca atctgctgca aaaggagata acgaaacgtt caagaaattc ttccacggct
      301 tgttgaaaga agggatttat atcgcaccat cggcttatga aacttggttt attaccgatg
      361 cgcttactta tgaagattta gattttacca ttcaagctat cgataaagtt tcaaaaacgt
      421 tttagtttaa gtagtcagaa aaaaataatg tcccattttg taaaatcaaa aagaattaac
      481 catgtaggat ataaaaggtc acataagcta gaatcttaaa tgaacttata tgtcttatat
      541 ggtaaaaaaa taggacatta ttttgttctc attaggacta gtcattattg ttttcttttc
      601 gatttcaata gtgccatttg atctggtgtt aggatttctt ttagcttttt attgtattcc
      661 tttcgttcct ctggagtaag tttgttagtt ccatttgttg gataattttt gaaaaggctc
      721 actaattgct gttcttgact ggagtctaaa ttcagttctt cgaccattct ttttacgcgg
      781 ttctcgtttt tttgttgcgg agagctagtt tgaaattgcc cttgattagg attttttttc
      841 ttttcttgtg caaagcttgt gaatccaatg cacaataatg cagctataat taattttttc
      901 ataactattt tttttagaag ttaataaggc tatttattct attaattttt taatcgaaaa
      961 tatataaaaa aaaaacttcc ggtaaccagc cggaagtttt tagacaaaca aattacacta
     1021 actattttaa ttaaccttgt ctttttcctt gatttctttc ttgttggttt ccatgacgat
     1081 tttctttaaa tctttcttga cgattttctt taaaatcttc tttcatagaa gtccattttt
     1141 cgaattgttt tgcattcaaa attttctcca ttcttttttt agtagctatt tgctcatcaa
     1201 gcattttcat tttcatagca aatcgttcat cattactagg cttaattcct ttttctttat
     1261 tggctttcat ttcagctttg tgagcttctt ttttagtcat tttctcagaa ataattgcgc
     1321 taatttcttt ttgttgcgaa tcattcaaat ccaattctaa agtcatcttt tttataatta
     1381 attggctttg ttgttctgga gtaaattgtt ccatttgatt tctttgaggc cttccattct
     1441 ttggttgagc aagcatagtt gtaccaacca ataataatgc caaaaccaat aaatttttgt
     1501 tcacaatttt taaaatgttt tcagataatt cttgggcatt cgttttcata tgtattgtgc
     1561 ttttttaaag ttataatact aggacattaa aaaggtttaa aggtttaatc tttaacaaat
     1621 aattatattt tattgttagc aatgcaccca aaatactttt ttaagtatga ttacaatcat
     1681 aatcaatcga ttaaactatc cgcaaatttg tattagaaat ttaaaaacta ttttttatgt
     1741 acaaattatc attatttatt gtagccttag ctttattttc ttcttgcggt aagaaggaaa
     1801 atgccaccga agacttttca acaggaggag cagcaactga aaaaacaaca gacccatcta
     1861 cttatgaccc aaatagaggt cttgggaaat attctacagt agaattagga gcccaactcg
     1921 atgcagcaat ggctggtgaa ggcgaaaaaa ttcaatctgt taaatgttct gcttgccata
     1981 aattgaccga cgaaaaatta gttgggccag gttggaaagg agtaacttct cgtaacaaac
     2041 cagaatggat tatgaacttt attaccaatc ctgacccaat gattgacaag gatccaaaag
     2101 ttcaagcgat gttagagctt tgtatggtgc gaatgccaaa ccaatcactt tcggatactg
     2161 acgccagaaa cgttttagaa tttatgcgca aaaacgacgg tgttaaataa gcagtagaga
     2221 aaccaattaa taaccagaat agtatgaaaa ataaattttt gaaagcgact ttagccataa
     2281 caatggtagc cgcattattt agttcttgca aacccaaaaa ttcaggtgat gttgtaagtg
     2341 gtgatgctgc gcaaaaagcg tatgtagctc ctggaaaata tgacgaattt tacaattttg
     2401 tttcaggagg atttagtggt caagtaagtg tttatggtct tccaagcgga agattgttga
     2461 gagtggttcc cgttttttcg gtagatccgc aaagtggtta cggctttagc gaagaaacaa
     2521 aaccaatgct aaacacctct cacggttttg tgccttggga cgatcaacac cataccgagt
     2581 tgtcacaaac caatggtgaa gtagatggtc gttggctttt tgccaatgcc aataataccc
     2641 cgagaattgc acgtttggat ttaaaaacat ttaaaacagc cgagattatc gagttgccca
     2701 acagtgccgg aaatcactct tcgccattta ttaccgaaaa cacggaatat gtagtagcag
     2761 gaactcgttt tagcgttccc tcagataatg ccaatggcga tgtaccaatc aatacctaca
     2821 agaaaaactt caaaggatat ttgagttttg taaaagtagg caaagaaggt caaatggata
     2881 ttgctttcca aattgaagca ccaggagtaa atttcgattt gagtcacgct ggtaaaggca
     2941 aatctcacgg atggttcttc ttctcttgtt acaatacaga acaagccaac actttgcttg
     3001 aagtgaatgc gtctcaaaaa gacaaagatt tcattatggc ggtaaactgg aaaaaagccg
     3061 aagaatacat caaagcgggt aaaggcaaaa aagtttctac caaatatgct cataatactt
     3121 gggacgagaa aacccatact gccaaatctg aaattagaaa cgaagttttg gtgttgagtg
     3181 ccaaagaatt gaaagatatt tgttatatga ttccttgtcc aaaatcgcct cacggttgtg
     3241 atgtggatcc aaccggagaa tatatcgttg gttcaggaaa attagccgca ttaattcctg
     3301 tatttagttt tgataaattg caaaatgcta ttaaaaacaa acaatttgac ggtgaatatg
     3361 acggaattcc agtcgtaaaa tatgaagcag ctcttcacgg tgaagttcaa aaaccaggtc
     3421 ttggtccatt gcataccgaa tttgacggaa aaggaaatgc ctataccaca ttcttcgttt
     3481 cttcagaagt ggtgaaatgg gacatcaaat cattgaaagt tttggataga gttccaactt
     3541 attactctgt aggtcacttg tgtattcctg gaggagacag tagaaaacca ttcggaaaat
     3601 acttgattgc ttacaacaaa attaccaaag acagatactt gccaacagga ccagaattgg
     3661 cccaaagtgc acaaatattt gacattagcg gcgacaaaat gcaattgatt ttggatttcc
     3721 caaccattgg agaaccccat tatgcccaag ctgctccagc cgatttgatt agaaataacg
     3781 gtcagttgaa attcttcaaa attgaagata ataaacaccc atttgcaacc aaaggagaaa
     3841 aagaagccaa agtggttaga aatggaaaaa gaattgatgt ctatatgact tccatccgtt
     3901 cgcactttgc ttccgataat atcgaaggaa taaaactagg agatgaggtt tatttccacg
     3961 taaccaactt agaacaagac tgggatgtgc ctcacggatt tgctataaaa ggagccgata
     4021 atgccgagtt gttaattatg cctggagaaa cctgtacctt gaaatggaca cctgaaaaag
     4081 tgggtatctt cccattctat tgtactgatt tttgtagtgc tttgcaacaa gaaatgcaag
     4141 gatatgtgag agtctcacca gcaggaagca gtgttccttt gacttttagc ttgggaacaa
     4201 atcttccagc tactaaataa ttaatttctt tactataaag ggaacaaagt ctctttgttc
     4261 ccttttttta taacaacaat tagtatgaac acttcaaaaa taactgtatt ttcaaaagta
     4321 tttcttttgg cagtgagtgc tttgtttttt gcatcactat tgtttccaat gtggcgaatt
     4381 gaactcgaag cgcctcaata tccagaaggt ttggtgttgc aattgcacgc caacaaaatt
     4441 ggtggcgatg tggatattat caacggatta aatcattata tcggaatgaa aaccttgcat
     4501 acggaagatt ttctagagtt tcaaatcatc ccttatatag ttggtttttt tggattattt
     4561 gctttggcaa tggtttttgt aaacaaacgg aaaggagtag ttttattatt cttgtctttt
     4621 ttacttttcg gagttttggc cggtgtggat ttttaccgat ggaactacga atacggacac
     4681 aatttgaatc ccaatgcagc cattgtggtg cccggaatgg cctatcaacc accattgatt
     4741 gggtataaac aactgcttaa ttttggggct tattccattc cagatattgg gggatggcta
     4801 ttgattgctg ctggaatttt gctttttatt attgtggcaa aagaaacccg tttgataagt
     4861 aaaattatta gaaaacctaa aatcaatcct gttttggttt tactattgtc tttttctttt
     4921 atgtcctgtg gcgattttaa agaagacccc atcaaattaa ataaagacaa ttgcgatttt
     4981 tgtaaaatga gcatcgccga cgggaaatat gcagccgaaa taattaccca aaaagggaga
     5041 atttataaat ttgacgacat tggttgtatg gttaattatt gcaaagagaa taccaacaca
     5101 aaaataggag cttattttgt aagcaacttt gctcaagaca atgttttaat tccagccgaa
     5161 accgcttttt tcctttcagg tggaacgatt caaagtccaa tgcacggtgg aattattgct
     5221 ttttcgtttg aaaaagaagc taaagaattt gcaacgaaat cgaatgcaaa accaatgaat
     5281 tgggcagcaa ttataaaaaa ataatgaaaa aaatcttcta catactgctt ttgttttctt
     5341 ccattcttca agcaaaagtg attgaagttg ggacaaataa acctattaaa tcaatccaaa
     5401 aagccattgt attggccaaa gtgggtgata cggttttggt tcacaaagga cattataaag
     5461 aaggtaatat catcatcaat aaaagaatag tttttattgg taaaaattac ccaattctag
     5521 acggacaaaa aaaatatgaa gtcttgtcca tcaaagccga tggcgtaatt gtgcgaggtt
     5581 ttaaagtcat aaaatcgggt tatgccacgc tcgaagatcc ttgtggaatc aaggtgtaca
     5641 ataaaaataa cgtggtcatt cagaacaata ttctcgacga taatttcttt ggaatctaca
     5701 ttcaaaacgg tgccaattgc attgtcaaaa acaacaaagt ttcggcttat ggcaaagaag
     5761 aacaacgcat cggcaatgga attcattgct ggaaaagcga ccgactccaa attatttcca
     5821 acaccatttc ggggcatcgc gatggtattt attttgaatt tgtaaccaat tcgacaatta
     5881 aaggtaattt ttctaccaag aatattcgtt acggattgca ttttatgttc tctaatgatg
     5941 atgcctatat ttcgaatgtt ttcaaaaaca atggtgccgg agtggccgtg atgtttaccc
     6001 gaagagtcaa gatggtcaac aatcattttg aagaaaattg gggtgatgcc gcttatggat
     6061 tgttcctcaa agaaatctct gatagtttta tcatcggaaa caaatttgcc aaaaacactt
     6121 cgggaattca tatggaaggc actagccgaa ttttggttca aaaaaatagt ttcgaagcca
     6181 atggttgggg aatgaaaatt caggccagtt gtatggataa tgaaataaaa aacaataatt
     6241 tcgtgggtaa cacttttgat attagtacta acggaagttt ggttctcaat actttcaacg
     6301 gcaattattg ggacaaatac gaaggctatg atttagacaa aaatggagtg ggtgacgtgc
     6361 catttcatcc cctgagtttg ttctctgtaa ttaccgaaaa taatccatcg gcgatgttgc
     6421 ttttcaggag ttttatgatt acgcttttgg acaaatccga gaaaattttg cccagcataa
     6481 ctcctgataa ttttatagat aaaaaaccat taatgcattc tttgccttta tgattgaaat
     6541 taaaaatata tttaaaaaat tcggaaaatt agaagttttg aacgatgtta atcttacttt
     6601 cagtaaagga gaatgcattg cactaattgg tccaaacggt tgcggaaaaa ccactttgat
     6661 aaaaagtatt ttgggaatgg ttattccgac taaaggtgat attttatttg ataaaaaatc
     6721 gatattgaat aagtataaat atcgagatca aattgggtat atgcctcaaa tagggcgtta
     6781 tcctgattat atgaccgtcg ggcaaattat cgaaatgatc aaaaaaattc gaaattctga
     6841 tgaagttttg gacgaagatt taataaaagc tttcgaactc gaaaaaatat tcgacaaaca
     6901 aatgcggact ctttcaggtg gaacaaccca aaaagtaagt gccattttag cttttttgtt
     6961 caatccacag gttttgattt tagacgaacc cacagcaggt ttagatccat tggcttccga
     7021 aattttgaaa gaaaaaatta tcaaagagag agaaaaaggg aaactcatct tgatcacttc
     7081 ccatcttttg agtgaactcg acgatatgat tagccaaatt atttttatgc aagacggcaa
     7141 agttcatttt cataaatcaa tctccgattt gttagaatct accaacgaac aaaaaatttc
     7201 aaaagctatt gccaaaattc taaaaagtga aagtcatgaa cagaatcatt aaaatcatat
     7261 ttctcgacat tctaaaaaac aagattgtat tggggtacac cttgattttg gccctgcttt
     7321 cttggagttc tttcggattg gaagacaatt ctgccaaagg attacttact attttgaacg
     7381 tcattttatt cacggttcca ttagtttcca tcctttttgc aacaatttat ttgtacaaca
     7441 gttccgaatt tatcgagctt ttgttaagcc agcctatcaa gcgaaaaaag atttggttga
     7501 gtttgttttt gggattatcg ctgtcaatgg tggctgcttt ttttattggt gctggaatcc
     7561 ctcttttgat aaatgcgccc gatagtgtcg gtttaatgat ggttattgta ggatgcttga
     7621 tttccttagt ttttgttgct ttggcttttt tgagttcaat tcttactcga gataaggcca
     7681 aaggaatagg gatttctata atgagttggt tgttttttgc cctgcttttt gatggaatgg
     7741 ttttgttctt gttgttccaa ttttcagatt atccgatcga aaaagctatg gtggctgtta
     7801 cggcttttag tccaatagat ttagctcgaa ttcaaatttt gttgcaactt gatgtttcag
     7861 caatgatggg ttatacgggg gctattttca aagatttttt tggcacggca atagggttgt
     7921 cagtttcgtt tttgttgtta tgtttgtggg taattatccc gttttgggtt tcactagaaa
     7981 agtttaaaaa taaagattta taaaaaaggt aaagaatgaa aacagatatc aaagatagag
     8041 ctgatataga gcaattggta gcgatttttt atggtaaaat aaaagaggat gcgtcaatta
     8101 gttatttttt taatgaggtt gccaaagtca attgggatga acatttgcct aaaatgtgtg
     8161 atttttttga aaacatattg ttcttttcgg gaaattatga gggtaatcca atggtggcac
     8221 acgaagattt acataaaaaa agtgaggtga gaggcgaaca ttttaatcac tggaatgctt
     8281 tatttgatgc tacagtagat gaattatttg aagggaaaaa agcagaggaa attaaacaaa
     8341 gatctgttaa tattgccgct gcaatgatgc agaaggcaca tcgataataa ctgggtttat
     8401 aggcctcaga tatagtttaa aactatgtac tttagtgcat tttgcgttag tttctaaaat
     8461 agtttaacca caaattacac aaatacttta accttgcaga ctttaagtcc atagtgattt
     8521 attccaataa aaaaagcgtg agttgcagta actcacgctt ttttggttaa tatatagaag
     8581 cgaattaata attttatttt ggaagcaata catcgccaat cacgtgaata attccgtttg
     8641 atgtaggaat ggaagccaca atttcggaac cattgacaaa ggttttgtct ccttttttgg
     8701 ttatttttac ttttccacca aagaccatat caaattcttg accgtcttgc atatactcgg
     8761 ttttcaaaga gccaacatag gtatgatagc ctaatatatt ttctaaatcg ccttttttct
     8821 ctggtttcaa taagccttca acagttccgg caggaagttt atcaaaagct gcattggttg
     8881 gggcaaaaac ggtaaaaggg ccagcattgc tcaaggcatc aacaagtccg gcagctttaa
     8941 cagcagcgac caaagtggtg tggtctttgc ttcctacagc tacttgaacg atatttggcg
     9001 cagaagtttc atctttaaca ccagattgtc ccattccagg agtttctgtc ggttctgttg
     9061 ttgtttcggg ggtggtttca ctgttttgct tgcatccaaa agcggcaaac ataaaagtag
     9121 ctagggatag gtaaattaat tttttcataa tgtaagtgct taagtttttt tataaagcaa
     9181 agcacttaat ttttaaacca ttattttatg agtgcaatca taaaagaggt ttgttttttg
     9241 cttttttaat agaaaaataa gcaacacatc caatgccaat caataaaaca atagcaattc
     9301 caaaaaggat ttcgtttaca taaggaatcg ggatgtaact gaaggctgca agaccttgaa
     9361 ttgccaaaac aatttcgttt agaatcactg cagtcgaaaa caatagtatt ccgtatttta
     9421 tgttcttgtt taggataatc aaatggttgg aataaatata aaacaataaa aacaaagaaa
     9481 tgattgccaa taaaacaaga tgtaaatagg ctattacaat aggtctaaag ccaaatgcca
     9541 gttggctaat gataggaact gttgagccca attgaagtat taattttaca cttaaagcaa
     9601 aacccacaaa cactaaaatg tatcttaaca aaggagagta ctgttctaaa gaatttattt
     9661 tagattttaa taaaacaaga aggaatttga accaagcaaa aacctgaatt atggcagcaa
     9721 ttatcgtaat cacataaagc cagaaaggta aatctaacca taaaatggat agaagatagg
     9781 ccggaatgca tgcaaagacg aataatttga agaaagtttc ataaaacgga tgttctgatt
     9841 ttcgaaggtt taaaaatccc aaaaacaatc ccatgctggc gaagaaaaac catccattgt
     9901 attgaaaatg caaataaaaa tagattgatg acaaatataa atcctgtaca acgtttttgg
     9961 atgccatcat ataggccaaa taaaaggtgc ccaaagaaga gagtatatta aaaaacagag
    10021 ctgctttcaa ccaatttttt accagtaaat catcttcaat taattttaaa tccttgaaat
    10081 attgatagca aaacacataa gaaactaaaa ctgaagctgt agagaaagtt atagaaaaga
    10141 aaccataccc ttgaataata aagcaaatga gcattccgta ggcacaaatc aagttagtaa
    10201 taattatttt attgtatttg atgatttgta aattcagtgt tttggtttga agataataaa
    10261 ccatcaatgt cattaatgta tggctaatcc aacctgaaaa agcaaaatga gaatgagaat
    10321 gttgcaaatg tttttggtca aaaaaaggaa attcaaatcc tattttgtat cgcatcaaaa
    10381 caccaagagt agcaacaata caaaggttaa gtagcgaaat tttaagccaa aatttagttt
    10441 tcaattgcat taataataga ttttgtgatg gatgatttct acctttttgg cttcgttcat
    10501 tttgctaaaa acacggatga cagtttcgac ccgaagtcca gtgtaattgg caatttcctg
    10561 acgagtaaat ggaattagga gtctttcggt agaatttcca gattttcttt tgtaagaatt
    10621 taaaaaagaa gttattctaa attcaggttt ttgattgata atatctttag aggtctttga
    10681 ttttgtgtga attttttggg caaataaaat caagaaatcc ttttgtatgt tgggatattc
    10741 gtctaaaatt tttaaaaact tatcttttga taatttaatt atggtactat cctggatggt
    10801 tattgcgtta gcaggatagg tttcgttaat aaacaaagga ggctctccaa aaccatctcc
    10861 atcacaaaat aatccttgag taaactcttt tccttcctca ttagaattaa acattctgac
    10921 gcttccttcc attatttgaa agtagaaatg agctgcttct ccttcttcaa aaataacttc
    10981 attttttttg tattttttgg caacagctcc ccaagaatat agtaaatcta aatctatctg
    11041 catatctaat ttcgaaagtt aattttttgt ttattttaaa ttaaatgggt aaaaacaagt
    11101 tagaaccgca aatttaattt ttataaagtt aaaaaaaata tgaccacaat cataagaaaa
    11161 aatttaattg aagctctata tttgcaacgt gaaaaagata atataccttt tggcgctatt
    11221 cattttagta aaaccggtac ttccggtgat agactatgct gttaattatg agtatatttc
    11281 gaaagtgctt tgcgttaata aagacaaacc caaaatgcat tgtaatggta aatgccattt
    11341 aatgaaagaa cttgccaaag catctgaaaa cgataaaccc atttcgtccg ataaaaaaac
    11401 tacctctcaa gaatcagaaa ttctgttttt tgaaaaaata aaaacatttc aattagcatc
    11461 aatttgtttt gatgattgcc caaaaacaaa cagtggctat tctgacttat actacttttt
    11521 gaatagtgat tccgttttta gaccgcccat ttttatttct taatttttca agtttacaca
    11581 aatactattt tcgatataga aacatatcga gaggatgttt attatcaaat tttaaaaaat
    11641 ataaaaatga aatttttaag aatactacca ttggttttat taaccattct tgcttcttgt
    11701 tcaaataatg aagaacccgc agttgatgaa ttgtatggat tgtctaagat taaagaaatt
    11761 acaaatacca ctcacaccat tgaattatac aaacatatgg gaggtttaga gcaaggttat
    11821 aacgaaattg cactaaaaat taaagacaat gcaacaaacc aatatataaa aaatgccacc
    11881 gttacttgga caccagtgat gcatatggcc atgatgaatc attcttgtcc taaatcgcca
    11941 gtaacaaaag tgtcaaccga tggattggtt tatgaaggtt atattgtttt tcaaatggct
    12001 caaaatgcaa ctgaatattg ggatttaaaa atagattaca ccatcaatgg aacaagttat
    12061 accatgactt ctgtaatcga tgttcctgca tcggcaaaac aaagagtgac tacttttaca
    12121 ggttctgatg gggttagata tatagctgcc tatgttgatc cgcatcatcc aaaagtgggt
    12181 gtaaacgata tggtagctgg agtttggaaa atgcaagaca tgatgacttt tccagttgta
    12241 gataattata aattgaaaat tgacccaaga atgccaagca tgggcaatca tagttcgcct
    12301 aataatgtag atttagttca aactgttgct ggtgatttat ataatggtaa actatcactt
    12361 actatgacag gttattggaa aattaatcta caattactca atgcagcagg aaccgttttg
    12421 aagggagaaa ccattaccga tgctgttcca gcaagtagta tttacttcga aattgagttt
    12481 taataaacaa ttattttctg ccagaatgaa ccattttggc agaaataatt tcacaatttt
    12541 taagatgaaa aactttataa tcataatagg attgtgtttt ttatttccca agatactttt
    12601 tgcccaagaa aaagaaacag atacaataac tccaaaagat ttaaccgaag taattgtaat
    12661 tgggaaaaag gcacaactgt atcaaaagca agccaaacca ttagctacaa ttgacgaata
    12721 tttgcagcaa tctaccaaaa tagatatgat tcgtcgaggg agttatgctt gggagcctat
    12781 gatcaataat atgcctaccg aaaggacttt ggtcaccatt gatggtatgc ggatttttgg
    12841 agcttgtacc gataaaatgg atcctataac ttcatatgtt gaggtttcca atttatccga
    12901 agcgacccta aaatcaggcc aacaaggcgc ttgtcacgga gcaacaattg ggggttctgt
    12961 tgatttacaa agaaatcgtg caggattttc taatttagga tggaatgcgg ctgtaaatac
    13021 aggttttgaa accaatagta aacaaaaaat agtaggatcg gctatcaatt atgcgaataa
    13081 ttcgttctat atagataccg attttatgtt tcgcgatgcc gagaattata cagcaggaaa
    13141 tggacaggaa gtcttgtttt cgcagtttag aaaattcaat atttcatcga cttctggttt
    13201 ttcttttgat aaaaacaaac tcttagaagg ttcaattatt tatgacaaag ctactgatgt
    13261 gggttatcct gctttgccaa tggatgtttc tttggccgaa gccttaatta cttcattgaa
    13321 gtttgaatat gttcctgagt cgtccccaat agaaaactgg gaaaccaaaa tttactacaa
    13381 taccatcact cataaaatgg atgacaccaa gcgaccttcg gttccgattc atatggatat
    13441 gcctggttgg agcgacacgt ttggatttta ttctaaagca aaaggaacgc ttagaagcca
    13501 tcattttata gccgatttga attcgtttta caatcgttct gttgccgaaa tgaccatgta
    13561 tccagccgat tctaacgaaa aattaatgtt tatgtacact tggcctgatg taaaaaccct
    13621 ttatacagct ttgtttttag aagataatat tgcgttaaat tgtcattcga gtttgaagtt
    13681 ttcaactagt ttgggtaatc atttcaacga ggttgctagt gaattgggtt tagaaagttt
    13741 acggattttt tatcccaata tgaaagctag taaaaaccga attttaccaa gcgtttcttc
    13801 taattattcg tttgatgaaa atggtttttc gtatggtttt ggattggctt acggtcagcg
    13861 tgctccatcg gtttctgaag gatatggttt ctatttgttc aatagttttg atacgtatga
    13921 ttacattgga aatccagaat tgaagaatga aaattcatta gaaacaagtg cttctatggg
    13981 gtataaaacc caaaaatgga gtgcaaaaat gtcggcttcc tattttcata tttccaatta
    14041 tattgttgga aaacccgatg cgagtttggt tccaatgacc attggagcaa gtggagttaa
    14101 aatttatacc gctttggatt atgctactat ttttaatact gacttaaatt tggagtatca
    14161 attttcagat tttatacaat ggaagggaca attggtttat agctacggaa aagattttga
    14221 aaaccagaat ttgccgttca ttagtccgct aaggtatttt tcttctttaa atttcaaaaa
    14281 agaaaaattt tcatcatcaa ttgctgttca aggtaatgca actcaaacgc aattcagccc
    14341 attttatggc gaagacagaa ctccatcata tgctattttg aattttgatg cgggatattc
    14401 tttagatttt gaaaaagtga aatgtggtct aaaagcagga gtcgaaaaca ttttagatac
    14461 ctattattcg actttttcag actggaacaa cattcctaga aagggaagga atttcttttt
    14521 gaatgtgagt tttagttttt gaaacaactt aaattaaaac cagtaaacaa gtaagataaa
    14581 aaatactgtt tactggtttt tctttttttt taactaagaa tttgtgttgt ctgtttttac
    14641 aaaaccgcca cgattttcaa cttggtcgat agattgctga acgattaaag ttccaatggt
    14701 gatcaagttt tgcaattcac acaagtcggc attaatttga tatttattta ttttttcttc
    14761 gatttcagtt ttccaaaggg ttagttcttc tttggcttga agcaaatcgg cgtcattgcg
    14821 cacaattcca gcattttgac gcatcaaaag ttgtaattct gtttttcttt tggcaacata
    14881 atctggattg atggctggtt tttcaattaa tttccaatcg gtcacttttc cttcgaattg
    14941 gtttttttcg aaagaagtag tagccaaata attaaaaatt ttatccgaat aaactagggc
    15001 ttctaacaaa gaattggagg ctaatctgtt ggctccgtgc aagccagttc tggaacattc
    15061 gccacaggca tataaatttt cgaccgaagt tttacccatt ttatccacca caattccgcc
    15121 acaaagataa tgttgagctg gaaccaccgg aatccaatct ttagacaaat cgattccaat
    15181 gcttttgcaa cgctcataaa tcattgggaa atgtttgatg aaaccatcca tatccaaatg
    15241 agaacaatcg agataaacac agtcgtcacc cgatttttta agttcttttt cgatgcattg
    15301 agaaacaata tcacgcgaag caagatcgcc tcgagaatca taatcgggca taaaacgatg
    15361 accgtttttg gtacgcaaat aagccccaaa accacgaacg gcttccgaaa tcaaaaagga
    15421 tgttccagtc gaagcatcgt ataaagcagt ggggtgaaat tgtatgaatt ccatctcttt
    15481 gattaccgca ttagctcgat aagccattgc aatcccatcg ccagttgcaa tgacaggatt
    15541 ggtggtatga ccatacagat gcccaattcc gccagtagca agtagagtaa aatcggattt
    15601 aaatgtcagg atttcattgg ttttttcgtc taaaacataa gctcctaaac aacgattgtt
    15661 ttcggtaatt aaatcaatgg ctaaatggta atctaaaacg agaatatttt ctttttgatg
    15721 tacttgatag gatatggcac gctgaatttc ttggccagtt tgatccttgt gatgcaccac
    15781 tctattttct gaatggccgc cttcttttcc taaatttaat gttccagcag catttttgtc
    15841 gaactttgct ccccattcaa ttaattcttt taatcgtttt ggaccttcgg taatcaccat
    15901 tcttactact tcttcgtcac acaatccatc accacaaatt agggtgtctt cgatgtgttt
    15961 ttcataggaa tcgacaattt tgtccgtcac gattgcaatg ccaccttgag catatttagt
    16021 gttggattcg tctgcattgg ctttggttac aatcgtgact tttttgtctg gaaattgttc
    16081 tgcaattttc agtgcaaagg tcagtccggc aacgccagag cctataatca agtagttggt
    16141 ttggatcatt atttagataa ttctagcatt cgttcaatag gaattaatgc ttttttcata
    16201 acttcatcag gaacagaaat ttcgggtgtt tcgtttaaca agcaatcata tactttttgc
    16261 aaagtgttca ttttcatata cccacattcg ctgcaagcac aagtgttgtc ttcgtttgaa
    16321 ggtgcaggaa ttaatatttt atgaggaact tcttgtttca ttttgtgaag aattcccgct
    16381 tcagtggcaa caataaattt ctgacttgga tctttcttta caaattcaat cattccagca
    16441 gtcgaaccga tgtagttggc cgtttttaga atatgggttt ctgattcggg atgcgcaatg
    16501 atttttgcgt cgggattttc tttgtataga gaaattaatt tgtctaaaga aaaagcctcg
    16561 tgaaccacac aggagccatc ccaaagtagc atttctctgc ctgtttgttc tattacatat
    16621 tttcccaaat ttttatctgg agcaaatatg ataggtttgt cttttggaat agattttaca
    16681 attttaactg catttgaaga agttacaaca atgtcagtca gggctttaac ttcggcagaa
    16741 caatttacat aagtaatcac aatatgatca ggatgtgcct tagtgaattt ttcgaaagta
    16801 tccgcaggac aagattctgc aagagaacaa cctgctttca aatcgggaag gattactttt
    16861 ttggaaggat tcaaaatttt tgcggtttcg gccataaaat gaactcctgc aaaaaggata
    16921 atatcagcat ctactttcgt tgcttcttgt gataatccta agctatctcc cacataatca
    16981 gcaacatcct gaatatcggc ttcttgataa tagtgcgcta aaataaccgc atttttctct
    17041 ttcttaagtt ctaatattcg ttctttaaga tttttcattc ctaagtcaat tttgtaatcc
    17101 tgttttatgg atattatttt tacaaagata caattatttg aaagacaaaa aactcaattt
    17161 aagtacggta ttttctgcat tctcattgga tttaacaaat tttaggcata atgtattatt
    17221 tgagaatttc gcatatgata aatatcagtt tttgtgttaa ttacatttac gacatttgca
    17281 gcttaaacca tataacaaaa atagttaagc agatgatgac aaataaacag ttacatactt
    17341 ttcatattcc agttatggga ttggctttca ccattgatag cccaattcga gtagcaaaat
    17401 acggaatatc atcagttata gcacttgcag atgatgagct tattgaaaaa atgagagctt
    17461 tttacagcga aaaattcgac gttccttatc aagaaataac aaaaaaaatt cacgattatc
    17521 gtgccgaaag gattacttct tatttgaact tggttgataa aatcgtaaaa gaaaaattcg
    17581 agaatttcaa gacagaattg tcagaaagta aacttgcatt agaaaattac atagcaatgt
    17641 tgcccaacaa atccgaaatc aaacaagggt tagaacactt cttggaagat ggaatcgcct
    17701 tgaaagaaaa tgttctcaaa tatctggaag aaaatcttta tcctggagat attgatgtga
    17761 atattatgac caaattagat aaagataact ttatcaataa cgagcaattg ccagtagaat
    17821 tcaatgatgc gcatgctgct cttcgcgggt ttgctaatag taatttgtct tcttcaatcg
    17881 tattatcagc agggatgaat cctagattat ttgcttattt cgaaagtttt ccttgctttt
    17941 ttccagatgc caacaatcag ttgaaaaaga aaattattct gaaagtgagt gattatcgtt
    18001 cggcaatgat tcaagggaat ttcttggcca aaaaagggct ttgggtttca gaatatcgta
    18061 tagaatcagg gttgaattgt ggcggacacg cttttgcaac agatgggatt ttattagggc
    18121 caattatgga ggaatttaaa cataaaaaag accaattggt gcaatcggct cacgatatta
    18181 tggtgaaagc cttggcgcaa aaaggaatgc acactcctga aactcctttg gaattgaaaa
    18241 ttacagttca aggtggtgta ggaacttcag aagagcacga ctttatgttg gattattata
    18301 atgttgatgc tgtgggttgg ggaacaccgt ttttgctagt tccagaagcc acttcagtag
    18361 atgtagaaac aagagaatta ttgcgaaatg ccaaagagga tgatttgtat ttgagtcata
    18421 tttcgccttt gggagttcca ttcaatactg ttagaggaac ctcaaacgag atgttcaaac
    18481 aaaagcgtat tgacgatgat aaagctggaa gttcttgccc taaaaagttt ttggcgttaa
    18541 gcaaagaatt tgggcccgaa ggaatttgta cttcttcaaa gaaataccaa gacgtaaaat
    18601 tggatgaatt aaatgaaata aaagaaactt taactcaaga agattatgat aaaaagaagt
    18661 tcgatattac cgaaaaagct tgtctttgcg taggtcttgc caatgcttct tatttggaaa
    18721 atgatattga gataaaaggt caggctcagg gtgtgattgt ttgcccaggt cctaatatgg
    18781 cttattttga caaggaagtt tcacttttgg aaatggtgaa acatatttat ggcaatgcca
    18841 atgtaatgac cgatttaaat cgccccaatt tatttgtcaa agaattgaaa atgtatatcg
    18901 attatttgaa aaatgacatc aaaaacatat cgtctgagat taatgcttcg caagtgaaaa
    18961 agttaaaagc ttttaaaagt aatttgttag aaggtatagc tttttatcaa aacttttttg
    19021 caaacaccca ttatttcgaa aatagtatca aagaaattca aaaccaattg gagttttata
    19081 ccaatgaatt ggttggagta gaagtgccag aattagtggt tgcttaaaga ggtttcttta
    19141 tataaaatca aactcgctta tttataggcg agtttttttt gctttaattt cgtttataaa
    19201 cttgatttaa atcaaaaaag attcgcctga attcacatca agcacaagtt cttcgaggca
    19261 agtgctttcg agcattttgg ctaaatcatt tctaatactc ttgaattttt tgttaagagg
    19321 acaaggatgt tgatcacaac aatgatttac acttaaccca caacttttaa aaatagaatc
    19381 cccatcaatg gcaattacaa tttgagcaag attgattctt ttgatatttt cttttggaat
    19441 ttcaaaaccg ccaccaattc ctttgataga gtgaacaatt tcttttttgg ccaaaatctg
    19501 caagattttt gctgtgaaag actttggcga atagatattt tcggcaatgt cttttatcgt
    19561 aactcttttg tcttgatagg attgtgcggc tataaaaata gaagcacgaa tggcgtattc
    19621 acaggttttt gaaaacataa tttttgtatt aaattagttt aaatacttta ttctcttaga
    19681 acaatttgta tcgtattggt tgataatttt tcggcatcaa ttctgttgtt cgatcgttcc
    19741 aattttgaaa gtgtttcaac atcgtttttg aagtattgaa tataataatt gccagaatag
    19801 tttttgcttt ccaaaaaacg aatcatttcc tttaaatctt cctctaacag caattctgaa
    19861 tgataggttg ttctgacttc aaaaggaatt ttggcttgta gaattaagtc aaatgttttt
    19921 tcgaaagtat catacaaatt agatttggta atggctttaa attttgattt tggagctttg
    19981 taatctaatg cgatataatc gagaaggcct ttgtttagga gtttttgtac tatgtctggc
    20041 gaacttccat tggtgtcgat tttaaccaaa aaacccattt ttttgacttc tgtaataaag
    20101 gaaatgatgt ttttgtgtaa taaacattcg cctccgctaa aaacaacagc gtctaataaa
    20161 ttttttcgag tatgtaaaaa tttaagaatt tcttcgaaag aaagacttcc ttttccgagt
    20221 acaatttctg gattatagca atacaaacac cgcatattgc agcctgcaaa ccaaagaatg
    20281 caggccgatt tatgaggata atctaataag gtgaaagatg ttaagctgta aataggagta
    20341 ctaacactta ccttcggtaa aatgagttcg ttgtttgtgt tcaccttttt tgccaatatt
    20401 aaaactttct acgggtctgt gatagcccat tacgcgtgtg taaaccaaac atttggttct
    20461 caaatgctga ttttcttcta aaataggatt ggtttttgtt tctgttttca tatgtttttg
    20521 ggtttaatgt tgttttgatt tttctttttt taaggtgtca tattgaatta gggcatcatc
    20581 acattttggg caatattcgt gttctccatt caaatagccg tgaacaggac aaacgctaaa
    20641 tacaggagtc actgtgatat atggcaatct aaagcttgaa actacttttt tgacaaactg
    20701 tttacaagct tcgggcgaac tcattttttc gctcatatac aagtgcaaga cggttcctcc
    20761 tgtatatttg cattgtaatt catcttgcaa aagcaaagct tcaaaaggat cgtcagtatg
    20821 atcagctgga atttgtgaac tgttagtgta ataaatattt tcgttttgcc cagcttgaat
    20881 aatatttgga aaacgttttt tgtcttcttt ggcaaaacga taagttgttc cttcggcggg
    20941 tgtggcttca aggttgtata aattgcctgt ttcttcctga aattctttca ttcgattgcg
    21001 aatatgatcc aaaatttctg atgcaaaatc aattcctgtt gtcgatgtta cggtgtcttg
    21061 ttttccagta aaattgacaa tcatttcgtt gattccgtta acaccaatgg tagaaaagtg
    21121 atttctaaaa tgtttcaaat aacgtttggt ataaggatat agtccgcgat cgtacatttc
    21181 ttgaatgaac acacgttttt tctctaaagt cgatttcgaa atgtataata atttgtctaa
    21241 ttgcttgaat aattcttctt tgtttcctct gtataaataa cccaatcgtg ccatatttat
    21301 ggttacaacg ccaatacttc cagtcatttc ggcacttcca aaaagaccgt ttccacgttt
    21361 taataattca cgcaagtcga gttgcaaacg acaacacata ctgcgaacag cattgggttt
    21421 ataggcattt tcattttcaa ctttttcgcc attttcgtct aatttgtatt ggcttccaat
    21481 aaaattctgg aaataggacg aaccaatttt ggctgtattt tcgaaaagta agtcggtatt
    21541 ttctccgttc caatcgaatt cttcggtaat atttacggtt ggaatcggga aagtaaaagg
    21601 ttgtccgtta gcgtcacctt cggtcattac tgtgtaatag gctttgttga taaggttcat
    21661 t
//
LOCUS       DAOSNN010000013        21527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_2442_length_21527_cov_5.496973, whole genome shotgun sequence.
ACCESSION   DAOSNN010000013 DAOSNN010000000
VERSION     DAOSNN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 21527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21527
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2442_length_21527_cov_5.496973"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..3075)
                     /gene="gcvP"
                     /locus_tag="PLQ70_01440"
     CDS             complement(226..3075)
                     /gene="gcvP"
                     /locus_tag="PLQ70_01440"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HQE33308.1"
                     /translation="MKTDAFALRHIGPRENDIQQMLKTIGVKSIEQLIYETLPDDIRL
                     KEPLNLDPAMTEYEYSNHIQQLGNKNKMFQSYIGLGYNQAIVPAVIQRNIFENPGWYT
                     AYTPYQAEIAQGRLEAILNFQTMVIELTGMEIANASLLDEGTAAAEAMALLFDVRSRD
                     QKKNNINKFFVSEEILPQTLSVLETRSTPIGIELVVGNHETFDFSSEFFGAILQYPGK
                     FGQVHDYTNFIANAKNNEIKVAVAADILSLAKLTPPGEMGAAVVVGTTQRFGIPLGYG
                     GPHAAYFATKDEYKRSMPGRIIGVTIDTDGNRALRMALGTREQHIKREKATSNICTAQ
                     VLLAVMAGMYVVYHGPKGLRYIANKIHSLAATLSNELGKLGFEQTNSAFFDTIVVKAD
                     AKKVKTIAEQNEINFYYIDENTVSISLNETTSIPDLNKIISVFASANNTQATAIETLT
                     ETNHFPANLERTSTFLEYDIFNKHHSETDLMRYIKKLERKDLSLNHSMISLGSCTMKL
                     NAAAEMLPLSNANWNNIHPFAPLDQVQGYQEMLSKLEQQLNVITGFAGTTLQPNSGAQ
                     GEYAGLMVIRAYHQSRGDHHRNVALIPSSAHGTNPASAAMAGMKIIVTKTLENGNIDV
                     EDLRQKAIEHKDDLSCLMVTYPSTHGVFESAIQEITKIIHENGGQVYMDGANMNAQVG
                     LTNPATIGADVCHLNLHKTFAIPHGGGGPGVGPICVAKHLVPFLPTNPVVSTGGENAI
                     SAISAAPWGSALVCLISYGYISMLGAEGLKSSTEHAILNANYIKEKLNGHYDTLYSGE
                     MGRAAHEMILECRPFKQKGIEVTDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESENLE
                     ELDRFCDAMISIREEIEASTLENKNNVLKNAPHTLAMLTADVWNFPYTREQAAFPLDY
                     IAENKFWPTVRRADDAYGDRNLVCSCAPIEAYVEN"
     gene            3214..4038
                     /locus_tag="PLQ70_01445"
     CDS             3214..4038
                     /locus_tag="PLQ70_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33309.1"
                     /translation="MKVLKRVFDFYIQSSIHVALSAYSLVQMTHFMFPIPNIKPVANF
                     AFFGTIVGYNFVKYEALARAKSHRKRDKLKSIAVLSFLSFLAVGFYFFQLQRLTQMVS
                     VAVFILTLLYTLPFFPNRKNARNWAGVKIYIVSFCWVGVTLILPILDAGISITMDFYL
                     KCMQRFILIFVLILIFEIIDLANDDPHLQTVPQQIGVRRTKNLGYILLLIFCVLEFFN
                     SKFNFHFFSLQLLLVFAIAIIVALFLAFVNENRSKYYTSFWVESVPILWWWLVLVF"
     gene            complement(4043..4495)
                     /locus_tag="PLQ70_01450"
     CDS             complement(4043..4495)
                     /locus_tag="PLQ70_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQE33310.1"
                     /translation="MTTINLTTKIKATKQIVFDVSRNIDIHQQSASKSNEIAIAGVTS
                     GLINLNETVTWKGKHFGFYLTHKSRITAMDLYSYFVDEMEEGKFKTFKHQHFFEETNG
                     VTTMTDQLHYETPFGIFGKLFDYLFLKKHLTQFILERNKILKVIAENS"
     gene            complement(4492..5403)
                     /locus_tag="PLQ70_01455"
     CDS             complement(4492..5403)
                     /locus_tag="PLQ70_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HQE33311.1"
                     /translation="MNKLIIAAGTGFLGQSLLDHFKDKFDKIVILTRGKNRTEGSIKY
                     VNWNAKTLTGWETELENATVLVNLAGKSVDCRYTKENKKEILLSRIQSTKILNQAVLK
                     CLNPPKHWLNSSTSTIYRFSLDKEMDEVNGEIGNDFSMNVAQSWEKAFFKTETPKTLK
                     TALRTSIVLGKKGGAFIPLKALAKLGFGGKQGKGNQFISWIHEKDFARAVDFIIEKEM
                     TGVVNVVSPKPILNFNFMKKLRKAVGFPFGIPINTFLLKIGSFFIRTEAELVLKSRNV
                     IPKRLLENGFQFKFGNIDTAFQNLLKK"
     gene            complement(5459..5845)
                     /locus_tag="PLQ70_01460"
     CDS             complement(5459..5845)
                     /locus_tag="PLQ70_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33312.1"
                     /translation="METTKILIGYAIYLPIALLLTYYVSKTLFKNGKIFMLDIFKGRE
                     DIAQATNKLFETGFYLLNIGFALMILEIQLYNNNYQELIEKLSYKIGGFSIYLGLMLF
                     FNLYFFFRGKRKAKETQTTDRLVFKA"
     gene            complement(5862..6209)
                     /locus_tag="PLQ70_01465"
     CDS             complement(5862..6209)
                     /locus_tag="PLQ70_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33313.1"
                     /translation="MNLNIIGYFIYLAITVFIILKVGKICYTNGNVYVAELIPNHKEI
                     CQKINQVLLLAYYLLNIGYCAMTLISWEKIISINQLIETIAIKTAIIIFIISILHYGN
                     IIIITKYIQKLIK"
     gene            complement(6206..6914)
                     /locus_tag="PLQ70_01470"
                     /pseudo
     CDS             complement(6206..6914)
                     /locus_tag="PLQ70_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
     gene            complement(6975..7478)
                     /locus_tag="PLQ70_01475"
     CDS             complement(6975..7478)
                     /locus_tag="PLQ70_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQE33314.1"
                     /translation="MEFKEAKNKFVQTWGALGSQWGINKTMAQIHALLMVSNEAVSME
                     EVMEELQISRGNASMNLRALMDWGIVYKEYKAGERREFFTAEKDLDELAVKIARERSK
                     REIKPALKILKEVSSIQSNNTAEETQFIEQTTKLYDFVLKADNVLDKITEYKDNWLTK
                     LVVKFMK"
     gene            complement(7605..9512)
                     /locus_tag="PLQ70_01480"
     CDS             complement(7605..9512)
                     /locus_tag="PLQ70_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQE33315.1"
                     /translation="MTTILDFWWVILLVLSIVLYKFVLRVFFGMVIVPEDRIGLVTKK
                     FVLFGSDKSLPDGRIIATKGEAGFQAKTLAPGLYWAMWPWQYSVNMTPFTIIPEGNIG
                     LILSKDGVEIPTGRILAQKVNSDNFQDATLFLDNGGQKGRQSAFITTGSYRINTYLFE
                     VVVAPQIVISENMVGIVTAMDGEPIPIGQIAGKFAENHNNFQDFDQFLKNGGNRGLQP
                     QVMLAGSYYINSWAIQIEQTPMTDVPIGYVGVVISYIGEDGKDVTGDTFKHGNIVSKG
                     QRGVWMEPFGPGKYALNKYTTKLEPVPTTNLVLNWANARSESHDLDKNLSTITVRSKD
                     GFPFNLDVAQIIHVPANEAPKVIARFGSMTNLVSQVLEPTIGNYFRNSAQDSDVISFL
                     STRKERQESAKKHIKAVLDEYNVNAVDTLIGDIVPPDSLMKTLTDRKIAEEEQKTYQT
                     QRMAQEQRQGVEKETAIADMQKEIVKASQSVEIAQRTADATVKKAEGDATSLKLNVNA
                     EAEATKMRANAEAEATKARAGAQAEATKLNASAEAEKISKTGLAEAEKIMAIGKSTAE
                     AYQLQVSAMGGDNFTKYKITEEIGKGNIKVIPDVLISGNSGSDGGISGLLGMKLMEMM
                     DTKNEKQEPKK"
     gene            complement(9801..11828)
                     /locus_tag="PLQ70_01485"
     CDS             complement(9801..11828)
                     /locus_tag="PLQ70_01485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQE33316.1"
                     /translation="MSILTQKFNTKHNTAPFSQIKLADYQPAFIENIAAAKAEIDAII
                     NNPEAPTFKNTIEALEFSGNALDRLSSIFFNLNSAETSDEMQKIAQEVSPLLTEFSND
                     ITLNEDLFQRIKTVYEQKESLNLSPEQATLLDKKFKSFSRNGALLSEDKKLKLREIDT
                     ELAKLKLTFGENVLAETNNYQLHITNESDLAGLPEGTVEAAKSLAKSKELDGWIFTLD
                     FPSYIPFVTYADNRELRKEIAIAAGKKAFQGNEFDNKEITLKIVKLRFERANLLGYET
                     HSHFVLEERMAQNPDKVKSFLNDLLTKAKPAAEKEFAELTAFAKEIDGIDQLEKWDGA
                     YYSEKLKQKLFNLDDEKLKPYFQLENVLNGAFTIAGKLYGLTFTEVFDIEKYHEEVTT
                     YEVKDEFGELVSIFYADFFPRKGKRNGAWMTSFKSQSIKDGVNERPHISNVCNFTKPT
                     ETKPSLLTFNEVTTLFHEFGHGLHGMLANTTYPSLSGTSVYWDFVELPSQVMENWCYE
                     PEALALFAKHYQTGEIIPQEYVEKIKESASFQEGMATLRQLSFGLLDMAFHSNNPTEI
                     KDIKAFEKTAFEGTTLYPDVAENCMSVSFSHIFQGGYSSGYYSYKWAEVLDADAFEYF
                     QQEGIFNKKVATKFKDNILSKGGTELPMELYKRFRGQEPKPDALLKRAGLI"
     gene            complement(11957..12442)
                     /gene="purE"
                     /locus_tag="PLQ70_01490"
     CDS             complement(11957..12442)
                     /gene="purE"
                     /locus_tag="PLQ70_01490"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQE33317.1"
                     /translation="MSKVAIIMGSISDMPIMQEAIDILKGFDIETEVDIVSAHRTPEK
                     LFDFSKNAHTRGISVIIAGAGGAAHLPGMVASMSPLPVIGVPVKSSNSIDGWDSVLSI
                     LQMPGGVPVATVALNGSKNAGILAAQIIGSSDKCVLDKIIAYKEGLKMAVNKAAEGLK
                     K"
     gene            complement(12665..13810)
                     /locus_tag="PLQ70_01495"
     CDS             complement(12665..13810)
                     /locus_tag="PLQ70_01495"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQE33318.1"
                     /translation="MNYFSSDFKLGILGGGQLGKMLLSDTRKFDIQTYILDPSDEAPC
                     KIACNQFFKGDLMDFDTVYNFGKKVDILTFEIELVNLEALEKLENEGLPVYPSPKTLR
                     LIQNKGVQKEFYSQHNIPTAPFQKFSNLENLKSEFSNLKLPFVWKCTEFGYDGNGVKI
                     VRTLQDLDNLPNVECIAEEMVPFKNELAVIVCRNPSGEIKTYPVVEMEFHPEANQVEY
                     VICPARIDDKVALKAREIALDVSEKFNHVGLLAVEMFQTQDDEILVNEVAPRPHNSGH
                     YSIEASYTSQFENHLRAVLDLPLGNTDSKVAGIMVNLVGAEGFSGDVIYENIETILGW
                     NGVTPHIYGKKQTRPFRKMGHVTIVNEDITVARKIAEDVKNTIRVIA"
     gene            14020..14547
                     /gene="hpt"
                     /locus_tag="PLQ70_01500"
     CDS             14020..14547
                     /gene="hpt"
                     /locus_tag="PLQ70_01500"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HQE33319.1"
                     /translation="MIQLHDKQFVPFISAQEIDFAIAKIAALVEDDFADEVPVFVGVL
                     NGAFMVVSDFMKHYKSPCEVSFIKMASYEGTTSTNEVKQLIGLDRDLSGRSVVIIEDI
                     VDTGNTLVALKDLFKKQNVKHFKVATLFFKPEALKNDIKIDYVGIRIPNKFIVGFGLD
                     YDGLGRNLPEVYQLK"
     gene            14596..15168
                     /locus_tag="PLQ70_01505"
     CDS             14596..15168
                     /locus_tag="PLQ70_01505"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQE33320.1"
                     /translation="MINIVLFGKPGAGKGTQAEFLKGKYNLVHLSTGDIFRFNIKNET
                     ELGKLARTYMDKGDLVPDEVTIKMLQSEVEKNQDAAGFLFDGFPRTIAQAEALDAFLA
                     TKNDKITATVALEANDEILVARLLERGKTSGRPDDQDEEKIRNRYQEYNEKTAPLMDY
                     YTKQNKFYAVDGIGSIADITERLSKVIDNL"
     gene            15248..16519
                     /locus_tag="PLQ70_01510"
     CDS             15248..16519
                     /locus_tag="PLQ70_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQE33321.1"
                     /translation="MEILIILFLIILNGVFSMSEIALISARKNRLETAAKKGNKNAQV
                     ALDLANSPNKFLSTVQIGITLIGILTGIYSGDKITHDVQDFVGGFEVLKPFAASLGVG
                     IVVVVLTFFSLVLGELLPKRIGLNHPEAIAKAVALPMKVISIVTAPFIWLLTHSTEFL
                     LNVLHIKPTADGKVTEEEIKAIIKEGTEGGEVQEIEQDIVERVFHIGDRKINSLMTHR
                     KSVVILPVHANKEQVKEFMLKELHSIYPVFGENHDDILGVVNLKNIFATIDKADFNLG
                     DIMTEAPFMMEYTTAYKALEQFKDSGVHYAFVSDEYGFFQGIITLNDILEALVGDASD
                     FYKDDFQLIEREDGSWLVDGHYSLHDFLTYFELDDLISDYEVTTVSGLIMTELSHIPK
                     EGEKLVWQKFELEVLDMDGVKIDKVMVKAIK"
     gene            16594..17595
                     /gene="obgE"
                     /locus_tag="PLQ70_01515"
     CDS             16594..17595
                     /gene="obgE"
                     /locus_tag="PLQ70_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQE33322.1"
                     /translation="MTEGNFVDYVKIFVSSGKGGKGSTHLHREKFIQYGGPDGGDGGR
                     GGHVVLVGNKGLWTLFHLKFARHVKAGHGGDGGGDRSTGADGEDKYIEVPLGTVVKDK
                     ETGETLFEITEDGEKKILCQGGKGGLGNWHFRSSTNQTPRYAQPGLPGLEMDVILELK
                     VLADVGLVGFPNAGKSTLLSVLTSAKPKIADYPFTTLKPNLGIVAYRDFQSFVIADIP
                     GIIEGAAEGKGLGHYFLRHIERNSTLLFLVPVDTPNIKEEYDILVNELTKYNPEMLDK
                     ERLLVISKCDMLDDELKAELKIQLDKEFKDIPYMFISSVAQQGLQDLKDKLWKMLND"
     gene            17825..19987
                     /locus_tag="PLQ70_01520"
     CDS             17825..19987
                     /locus_tag="PLQ70_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQE33323.1"
                     /translation="MKKFIFALIAGILLLPNSVKADEGMWFLMFIERLNHRDMEKMGL
                     QLTSEEIYSINHSSLKDAIVQFNGGCTAEIVSKEGLVLTNHHCGYSAIAELSTAEQNY
                     LKDGFWAKDKSAEMKPKSLYVRFFVRMDDVSKRILLKVNDKMSETDRNKVIQQEIALI
                     EKENSEGGKYTVSVRPFFQGNEYYYFVYQDYKDVRLVGTPPESIGKFGGDTDNWEWPR
                     HTGDFSMFRVYADKNGNPAEYSKDNLPLKPKHYLPISLKGVEENDFAMILGYPGRTNR
                     WMPANGINQNVNFAYPAWVEGAKMGMDRMKVYMDKDETVSLQYASKYASTANYWKNRQ
                     GMIDALTKSGTVETKTIEESKFNDWANKSENKAKYGQVVATINDYYAKTNLKARHDNY
                     LSQLMRTATYGSAPANLGNSLLYYFNENEAKRAEVLPKINALIESVYGKFYAPLEKDV
                     LIAQLNLYTSKGAEFGLSPMISKLKEDNKGDFATVVNEAVFKSIFTSKETVLAFLNNP
                     KPEAISADPLYVLSNDLLTKVRAKSEDQQQMDDNFEKAYRNLVEGLRESKINSIQYPD
                     ANSTLRLSYGKVNALPKDPRNDAKVNNYTTMTGLVKKYKAGDEEFDLPARLLELNKAK
                     DFGKYADKDGYMPINFLTDNDITGGNSGSPVLNGKGELIGIAFDGNIEAMAGDVIFDK
                     KLQRTISVDIRYVLWLIDKYAGAKHIVDEMTIAGENLK"
     gene            complement(20168..21157)
                     /locus_tag="PLQ70_01525"
     CDS             complement(20168..21157)
                     /locus_tag="PLQ70_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HQE33324.1"
                     /translation="MKIFYAIQATGNGHISRATQLYPYLQQFGEVDFFLSGNNASLDI
                     NLPIKFRSAGCSLHYSKCGGLNYWDIVKNIRPHQIYKDATALPLKNYDIIINDFDSVT
                     ALACKMQKVHSVQFGHQASFVSEHTPRPEKRSLMGEMILKHYAPSPKHIGLHFENYDS
                     FIHPPVIKDEITQAEPKDLKHITVYLPSFQKDCLEKAFHKLSDVEFHWFLNDVQTKHK
                     VGNITYFPVNQKLFNESLINCHGIITGGGFETPAEALFLRKKVLSIPIRDHYEQECNA
                     AALKKMGVPVVYEVGNDFDLIIENWLNSVVIYPKMKANTINETLQFLFDTYRD"
     gene            complement(21256..>21527)
                     /locus_tag="PLQ70_01530"
     CDS             complement(21256..>21527)
                     /locus_tag="PLQ70_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQE33325.1"
                     /translation="FETTAAEIAIEKKYSYVVCGHIHMPQKKVVKNQHGEVLYLNSGD
                     WIENLTALEYKKEKWKIYQYKKSDFSQLEPKEEVVYNDIVSKILS"
BASE COUNT         6647 a         3664 c         3838 g         7378 t
ORIGIN      
        1 tacctctgtc aaaaaggatt tgttcccaaa aatcaaccag ttccaataaa tgtggtccta
       61 aatctatttt tgctgttttg ataaaaatat aactgatttt gctattggca agtaatttca
      121 aatagaattc actcatcaat aaatgcaaat cctcgcgtgt ttgaatgtct gtcatagttt
      181 tttaaataaa aatgtctcga tttctcgaga catttcagtt gtattttaat tttctacgta
      241 agcctcaatc ggcgcgcaac tacaaaccaa gtttctgtct ccataagcgt catcggctct
      301 acgaacggtt ggccagaatt tgttttctgc aatataatct aatgggaaag cggcttgttc
      361 tctggtgtat ggaaagttcc aaacatcagc tgtaagcatt gctaaagtat gcggagcgtt
      421 tttcaaaaca ttgtttttgt tttctaatgt tgaagcttca atttcttctc tgatagaaat
      481 cattgcatca caaaaacggt ctaattcttc tagattttcg ctttcagttg gttcaatcat
      541 caaggttcca gcaactggga aagataccgt tggtgcgtgg aaaccataat ccatcaaacg
      601 tttagcaata tcagttactt cgataccttt ttgtttgaaa gggcgacatt ctaagatcat
      661 ttcgtgagct gcacgaccca tttctccaga atatagagtg tcgtaatgtc cgtttaattt
      721 ttctttaata taattggcat tcaaaatggc gtgttcagta gagcttttta aaccttcggc
      781 acccaacatc gaaatataac cgtaagaaat taagcaaacc aaagccgaac cccaaggagc
      841 agcagaaata gcagaaatag cattttcgcc accagttgaa actactggat ttgttggtaa
      901 aaacggaact aaatgtttag ctacacagat tggtccaaca cctggtccac caccaccgtg
      961 aggaatggcg aatgttttat gtaaattcaa gtggcaaacg tcagctccaa tggttgctgg
     1021 atttgttaat ccaacttgtg cattcatatt ggctccgtcc atatagactt gaccaccgtt
     1081 ttcgtgaata atttttgtaa tttcttggat agcactttca aaaacaccgt gagtcgaagg
     1141 ataagtaacc attaaacacg acaaatcgtc tttgtgttcg atagcttttt gacgtaaatc
     1201 ttccacgtcg atatttccgt tttctagtgt tttagttaca attattttca ttcctgccat
     1261 tgctgccgaa gcaggattgg ttccgtgtgc tgatgaagga ataagagcaa catttctatg
     1321 atggtcacca cgagattgat gatatgcacg aattaccatc aatcctgcat attctccttg
     1381 tgctccagaa ttgggttgca aagtagttcc tgcaaaacca gtaattacgt ttaattgttg
     1441 ttcgagtttt gacaacattt cttgataccc ttgcacttga tctaatggag caaaagggtg
     1501 aatgttgttc caattggcat tgcttaatgg caacatttcg gctgcagcat tcaatttcat
     1561 ggtacaagaa cctaacgaaa tcatcgagtg atttagggat aaatctttgc gttctagttt
     1621 tttgatataa cgcatcaaat cggtttcgga atggtgtttg ttgaaaatat catattccaa
     1681 aaatgtagaa gttctttcta aattagcagg aaaatgattg gtttctgtta aagtttctat
     1741 tgctgttgcc tgagtattat ttgcagaagc gaaaacagaa ataattttat tcaaatcggg
     1801 aatacttgta gtttcgttca aagaaatgga aactgtattt tcgtcaatat agtagaaatt
     1861 aatttcgttt tgttctgcta ttgtttttac ttttttggca tcggctttta caacaatggt
     1921 gtcaaagaaa gcggagttgg tttgctcgaa acctaatttt cctaattcgt ttgacaaagt
     1981 tgcagccaat gaatggattt tattggcaat atatctcaag ccttttggtc cgtgatacac
     2041 tacatacatt ccagccatta cggctaataa aacttgggct gtacagatat ttgaagttgc
     2101 tttttctcgt tttatgtgtt gttcacgtgt tcctaaagcc attcgaagcg cacgatttcc
     2161 gtcggtatct attgttacgc caataattct tcctggcata cttcttttgt attcgtcttt
     2221 tgtggcaaaa taagcagcat gtggtccgcc ataacccaac ggaattccga aacgttgcgt
     2281 ggttcctact acaacagctg ctcccatttc tcctggagga gttagttttg ctagacttaa
     2341 aatatcggca gcaacggcta cttttatttc gttgtttttt gcattggcaa taaaattagt
     2401 atagtcgtga acttgtccaa atttgcccgg atattgaagg atggctccaa aaaactctga
     2461 agaaaaatca aaagtttcgt ggtttccaac aaccaattct attccgattg gagttgaacg
     2521 agtttctaaa accgataaag tttgtggtaa aatctcttct gatacgaaga atttattgat
     2581 gttatttttc ttttgatcac gagaacgaac gtcaaataac aacgccatag cttcggctgc
     2641 agctgttcct tcgtccaata aagaagcgtt ggcaatttcc attcctgtca attcaataac
     2701 catcgtttgg aaattcaaaa tagcttctaa tcgaccttgt gcaatttcgg cttgataggg
     2761 tgtgtaagcg gtgtaccaac ccggattttc aaaaatattt ctctgaataa cagcaggaac
     2821 aatagcctga ttgtagccta aaccaatata ggattggaac attttatttt tattgcccaa
     2881 ttgttgaata tgattggaat actcatattc ggtcattgct ggatctaaat ttaggggttc
     2941 ttttaaacga atatcatctg gaagtgtttc ataaatgagt tgttcgatgg attttactcc
     3001 aattgttttc aacatttgtt gaatgtcatt ttctcttggc ccaatatgtc ttaaagcaaa
     3061 agcatctgtt ttcatagaaa taaaaaaagg attgtttttt aaaggcgcaa atttacgcag
     3121 aatattcaaa agaaaaggac ttcaaggatt tgatttttag gaatctttca actaaagtgg
     3181 ctagttgtta acaacgttgt ttatttttgt gagatgaaag ttttaaaaag agtttttgat
     3241 ttttatattc agagtagtat tcacgttgct ttgtcggctt attctttggt acaaatgact
     3301 cattttatgt ttcctattcc aaatattaaa ccagtggcta attttgcttt ttttggaact
     3361 atcgtgggct ataattttgt aaaatatgaa gctttagcca gagccaagag ccatcgaaaa
     3421 agagacaagc ttaaatctat tgctgttttg agttttttga gttttttggc agttggtttc
     3481 tacttttttc agctgcaacg tcttactcaa atggtttctg ttgcggtttt tattctgaca
     3541 ttattataca cgcttccatt cttcccaaac cgaaaaaatg ctcgaaattg ggctggtgta
     3601 aaaatctata ttgtgtcttt ctgttgggtt ggagttactt tgatattacc aattttggat
     3661 gcaggcattt ctattacgat ggatttttac ttaaaatgta tgcagcgatt tatcttaatc
     3721 tttgtgctaa tattgatttt tgaaatcatt gatttagcta atgatgatcc tcatttgcaa
     3781 accgttccgc agcaaatagg agtaaggcga actaaaaatt taggctatat tttgttgcta
     3841 attttttgtg tattagaatt ttttaatagc aaatttaatt ttcatttttt ttcattacaa
     3901 ttgctattgg tttttgccat tgcgattatt gtggctttgt ttttggcttt tgtcaacgaa
     3961 aaccgatcga aatattacac ttctttttgg gtagaaagtg tgcctatttt gtggtggtgg
     4021 ttggttttag ttttttaaat tttcaactat tttccgctat aactttcaat attttatttc
     4081 tttccaaaat aaattgtgtc aaatgttttt tcaaaaacaa ataatcaaat aatttcccaa
     4141 aaataccaaa cggagtttca tagtgcagtt gatctgtcat tgtggtaact ccgtttgttt
     4201 cttcaaaaaa atgctgatgt ttgaaggttt tgaattttcc ttcttccatt tcatccacaa
     4261 aatagctgta taaatccatt gctgtaattc ggcttttatg agttaaataa aatccaaagt
     4321 gttttccttt ccaagttacg gtttcgttta gatttatcaa tccagaagtt acaccagcaa
     4381 tggcgatttc atttgatttg cttgcagatt gctgatgaat atcaatgttt ctggaaacat
     4441 caaaaacgat ttgttttgtg gcttttattt ttgtggttag gtttatggtt gtcatttttt
     4501 taataaattt tgaaaagcag tatcaatatt tccgaacttg aattggaatc cattttctaa
     4561 aagtcttttc ggaatcacat ttctactttt tagaaccaat tcggcttctg ttcgaatgaa
     4621 aaaagatcca attttcaaca aaaaagtatt aattggaata ccaaaaggaa aaccaactgc
     4681 ttttcgtagt tttttcataa aattaaaatt tagaattggt tttggagaaa ctacatttac
     4741 aactccagtc atttcttttt cgataataaa atcaactgct cttgcaaaat ccttttcgtg
     4801 aatccagctt ataaattgat tgccttttcc ttgtttgccg ccaaagccca actttgccaa
     4861 agcctttaac ggaataaaag cacctccttt tttccctaaa acgatagaag ttcgcaaagc
     4921 tgtttttaag gttttgggag tttcagtttt aaaaaaagct ttctcccaag attgagccac
     4981 attcatcgag aaatcattgc caatttcgcc attaacttca tccatttctt tgtctaatga
     5041 aaacctataa atggtagatg ttgatgaatt cagccaatgt tttgggggat ttaagcattt
     5101 taaaacggct tggtttaaaa ttttggtact ttgaattcga gataataaaa tctctttttt
     5161 attctctttg gtatagcgac aatcaactga ttttcctgca aggtttacta aaactgtcgc
     5221 attttctaat tcagtttccc agccggttag cgtttttgca ttccaattga cgtattttat
     5281 actgccctcc gttctgttct ttccacgagt aagaatgacg attttgtcaa atttatcttt
     5341 aaagtgatcc agcagacttt gacctaaaaa tccagttcct gctgcgataa tgagtttgtt
     5401 cattttgtcg gatgttaaac ctaacaggtt tttgaaacct gattggtttg ttaggcaatt
     5461 aggctttaaa tactaatcta tctgtggttt gagtttcttt ggcttttctt tttcctctaa
     5521 aaaaaaagta taaattaaag aacaacatca gtccaagata aatagaaaat ccgccaattt
     5581 tgtaacttaa tttttcgatt aattcctgat agttgttatt gtataattga atttcgagaa
     5641 tcatcaaagc aaacccaatg ttgagtaaat aaaacccagt ttcgaagagt ttgttggtgg
     5701 cttgtgctat atcttctctg cctttgaaaa tgtcgagcat aaatattttt ccgtttttga
     5761 aaagtgtttt agaaacataa taggttagta ataatgcaat tggtaaatag atggcgtagc
     5821 cgataagaat ttttgtagtt tccataatgt tatttttaat attatttgat taatttttga
     5881 atgtatttgg taatgattat aatgttcccg taatgtaata tcgaaatgat aaaaatgata
     5941 attgctgttt ttattgcaat ggtttcaatg agttgattaa tcgaaattat tttctcccac
     6001 gaaatcaaag tcattgcgca atacccaatg ttgagtagat aatacgccag taacaaaacc
     6061 tgatttattt tttggcagat ttctttgtga tttgggatta attcagctac ataaacattg
     6121 ccattagtgt agcagatttt acccactttt agaataatga aaaccgtgat ggctaggtaa
     6181 atgaaatagc cgatgatgtt gaggttcata ataatttctt tttattttca actgttacgg
     6241 tagagccttt tgcaaggatg acagaagttg gttccaggtt ttgaagaaaa acaaagtcat
     6301 taccgtacac attttcaaag tcaacatcaa ttttgtagct gacgacttgt aattgttgcc
     6361 atcttgggtg ttttacttca tattcaaaag tgatgttttt atttgttttt gtataaccaa
     6421 aatagtgctc tgtgatgaat tcagcttcag aattatgatc aatttcaatg ctgttttttt
     6481 ctgttgctac taaaattgaa ttccactcat tattgttttt ccattgataa acaaaatcaa
     6541 ttgaattttt attttcagtg gcagaatgtt tcataggcat cgtttgataa tgctctttgt
     6601 aaatagtatt tgctacaaaa gttaaggctg gcttcggcac aatttcttta ataaaagcaa
     6661 caccgcgttt ccattctcca ttctcgaagc gttttacata gaatctcaaa tttacttctt
     6721 caaaattgct gtgaaacgga attttaaacc cgattaattt cacattgtta aacataaaac
     6781 caactaaact tacataacat ttgttattcc ataaatcaat ttctgttcct tttgggaggt
     6841 gtttttaata gtttagcatc aatttcataa ttgattaagg ctaggttttt ccattcggcg
     6901 gttaaaaagc tcatagttct ttatgtttta aactttcaga aaaaaatgaa agttttggtt
     6961 aaattttttt aattctattt cataaactta accactaatt tggtaagcca attgtctttg
     7021 tattcggtaa ttttatccaa aacattatca gctttcaaaa caaaatcgta aagtttggta
     7081 gtttgctcaa taaactgagt ttcttcagca gtattattag actgaatcga tgaaacttct
     7141 tttaaaattt ttagcgctgg tttaatttct cttttgcttc tttctctggc gatttttacg
     7201 gccaattcgt ctaaatcttt ttcggctgta aaaaactctc ttctttcgcc tgctttatat
     7261 tctttataga caatgcccca atccatcaaa gctctcaaat tcatactggc attgccacgg
     7321 gaaatttgca attcttccat tacttcttcc atagaaacag cttcgttaga caccatcaac
     7381 aaagcgtgaa tttgcgccat agttttgtta atgccccatt gcgagcctaa tgctccccag
     7441 gtttgtacaa acttattttt tgcttctttg aattccatat agcaaatgta agtaaaagtt
     7501 tttaaacttt caaaaataaa tgaaagtttg ttttaaaaaa accgaagcat tgctacttcg
     7561 gttttacatc tgctcccgaa attcgggact aaattctaaa aaaattattt tttaggttct
     7621 tgtttttcgt ttttggtatc catcatttcc attagtttca tccctaaaag accactaatt
     7681 ccaccatcgg agcctgaatt tcctgaaatt aaaacatctg gaatcacttt aatgttgccc
     7741 tttccaattt cttctgtaat tttatatttg gtaaagttat cgccacccat tgcagaaact
     7801 tgcaactgat aagcttcggc agtagattta ccaattgcca taattttctc agcttcggcc
     7861 aaacctgttt ttgaaatttt ttcagcttct gcactggcat tcagtttggt tgcttcggct
     7921 tgggctcctg ctcgagcttt ggttgcttct gcctcggcat tggcacgcat tttagtagct
     7981 tcggcttctg catttacatt caattttaaa ctggttgcat ctccttcggc ttttttaacg
     8041 gttgcgtcgg cagttcgttg agcaatttct acactttgag aggcttttac aatttccttt
     8101 tgcatatcgg caatggcggt ttctttttca acaccttggc gttgttcttg tgccattctt
     8161 tgtgtttgat aggttttttg ttcttcctcg gcaatttttc tatcggtcaa tgtcttcatt
     8221 aaagaatctg gtggcacgat atcaccaatt aaggtgtcaa cagcattcac gttatattcg
     8281 tctaaaactg ctttgatgtg tttttttgct gattcttgac gttcttttcg agtgcttaag
     8341 aatgaaatta catcactatc ttgtgccgag tttctaaaat agttaccaat ggttggttcc
     8401 aaaacttgtg atactagatt ggtcatactt ccaaaacgag caattacttt tggagcctca
     8461 ttagctggga cgtgtataat ttgtgcaaca tctaaattaa aagggaaacc atctttagaa
     8521 cgaactgtaa tcgtggatag atttttatct aaatcgtgag attcgctgcg tgcgttagcc
     8581 caattcaaaa ccaagttcgt tgtaggaaca ggttctagtt ttgtagtgta tttattaagc
     8641 gcatattttc ctggaccgaa aggttccatc caaacgccac gttgcccttt tgaaacaata
     8701 tttccgtgtt tgaaagtatc tccagttaca tcttttccgt cttcgccaat ataagaaatc
     8761 acaactccta cataaccaat aggtacatcg gtcatagggg tttgctcgat ttgaattgcc
     8821 caagagttga tgtaatagga acctgccaac attacttgtg gctgcaatcc acgatttcct
     8881 ccatttttca aaaattggtc aaaatcttga aaattgttat gattttcagc aaatttccct
     8941 gcaatttgtc caattggaat aggttcgcca tccattgcgg ttacaattcc caccatattt
     9001 tctgagatga ctatttgtgg agctactaca acttcaaata aataagtatt aatacgataa
     9061 gatcccgttg tgataaaggc agattgtcgc cctttttgtc cgccattatc tagaaacaaa
     9121 gtggcatctt gaaaattgtc tgagtttact ttttgagcta aaattcgacc tgttggaatt
     9181 tcgactccat ctttgcttaa tatcaatccg atatttcctt ctggaataat tgtaaaaggg
     9241 gtcatattta cagaatattg ccaaggccac atagcccaat acaacccagg ggctagtgtt
     9301 ttagcttgga aaccagcttc ccctttggtc gctataatcc ttccgtcagg taaggattta
     9361 tctgatccaa aaagcacaaa ttttttggtt actaaaccaa ttctgtcttc aggaacaatt
     9421 accatcccaa agaaaactct taaaacaaat ttgtaaagaa caatggatag tacaagtaaa
     9481 attacccacc aaaaatctag aattgttgtc attattaaat agtttattga ttgttaatgt
     9541 gtttgatttt tataaataat gaccatagaa aaatagcgta atttcctttt gttaagttta
     9601 tgaattcaaa tttgtatatg aaattttaaa ttcctcctga agttacaaca gctttgtttt
     9661 attttagaat atcgttatgg tataaaatct cttattatac ggaaacactt attaattgtt
     9721 acaaaaaaat aagaaatatt ttagatataa aaaaaccgaa gcattgctac ttcggtttct
     9781 atcggcattc taaaatcttt ttatattaac ccagctcttt tcaataaagc atctggtttt
     9841 ggttcttgtc ctctaaaacg tttgtacaat tccatcggca attcagtccc tccttttgaa
     9901 aggatgttgt ctttgaattt ggtagccact tttttattga aaatgccttc ttgttgaaaa
     9961 tattcaaaag catcggcatc gagaacttca gcccatttgt agctgtaata gccagaagaa
    10021 taaccacctt gaaaaatatg cgaaaacgaa accgacatgc aattttcggc cacatcagga
    10081 tataaagttg ttccttcaaa agcggttttt tcaaacgctt tgatgtcttt tatttcggtt
    10141 ggattgttgc tatggaaagc catatccaat aagccaaaac tcaattgacg caaagtcgcc
    10201 attccttcct gaaaactggc actttctttg attttttcta catattcctg cggtataatt
    10261 tcacccgttt gataatgttt cgcaaataat gccaaagctt ccggttcgta gcaccagttt
    10321 tccattacct ggcttggcaa ttctacaaag tcccaataaa ccgatgttcc agataaactt
    10381 gggtaagtcg tattcgccaa cattccatgc aatccgtgac caaattcgtg gaacagcgtt
    10441 gttacttcgt taaaagtcag caaagacggt ttggtttcgg taggtttcgt aaaattacaa
    10501 acgttagaaa tatgcggtct ttcgtttaca ccatctttta tagattgcga tttgaaagaa
    10561 gtcatccaag cgccatttcg ttttcctttt cttggaaaga aatcggcata aaaaatagaa
    10621 accagttcgc caaattcgtc tttcacttca taagtagtta cttcttcgtg gtatttttca
    10681 atgtcaaaca cttcggtaaa agtcaatccg tataattttc cagcaatagt aaaagcacca
    10741 ttcaaaacgt tttctaactg aaagtaaggt ttcaactttt catcatccaa attgaataat
    10801 ttttgtttca atttttcaga ataataagcg ccatcccatt tttctagctg atcaattccg
    10861 tctatttctt tggcgaaagc cgttaattct gcaaattctt tctcagctgc aggtttggct
    10921 ttggttaata aatcattcaa aaacgatttt actttatcag ggttttgcgc cattctttct
    10981 tctaaaacaa aatgcgaatg ggtttcataa cctaataaat tggctctttc gaaacgcaat
    11041 ttcacaattt ttaaagtgat ttccttgttg tcgaattcgt ttccttgaaa tgctttttta
    11101 ccagctgcaa ttgctatttc ttttctcaat tcacgattat ctgcataagt cacaaacgga
    11161 atataacttg ggaaatccag agtaaaaatc cagccgtcca gctctttgct ttttgccaat
    11221 gatttagcgg cttcaaccgt tccttctggc aaacctgcca aatcactttc attcgtaata
    11281 tgcaattgat agttgttggt ttcagccaaa acattttcgc caaaagtcag tttcagtttg
    11341 gctaattctg tgtcaatttc acgcaatttt aattttttat cttccgaaag taaagcaccg
    11401 tttctggaaa aacttttgaa ttttttatcc aataatgtag cttgttctgg agataaattc
    11461 aggctttctt tttgttcgta aaccgttttt atcctttgaa ataaatcttc gttgagcgta
    11521 atgtcattac taaattcagt caataatggc gaaacttctt gagcaatttt ttgcatttcg
    11581 tcactcgttt cagccgaatt caaattaaag aaaatagacg acaaacgatc caaagcatta
    11641 cccgaaaatt ccaaagcttc gatggtgttt ttgaaagtcg gagcttcagg attattgatg
    11701 atagcatcaa tttctgcttt tgctgcagcg atgttttcga taaaagcagg ttggtaatcg
    11761 gctaatttta tctgcgaaaa gggtgcagta ttgtgtttgg tgttgaattt ttgagttaag
    11821 atgctcatat tatattgttt tgttacgcaa agattcacaa agaaaatcac agaggcacac
    11881 aaagtttttt taaataagtt ctattttatt ctttgcgaat cttggtgtgt tctttgtgaa
    11941 tctccgtgaa atgtttttat tttttcaatc cttcggctgc tttatttaca gccattttca
    12001 atccttcctt ataggcaatg attttgtcta aaacacattt gtccgagctg ccaataattt
    12061 gagctgctaa aatccctgca ttttttgagc cgttcaaagc cacagtggca acaggaacac
    12121 cacctggcat ttgcaaaata gacaaaaccg aatcccaacc atcaatagaa ttgcttgatt
    12181 ttacaggaac tccaatcaca ggtagtggcg acatcgatgc caccattcct ggtaaatgtg
    12241 cagccccacc agctccggca ataattaccg aaatgccacg agtgtgggca tttttgctaa
    12301 aatcaaataa tttttcgggt gttctgtgtg ccgaaacgat atcaacttct gtttctatgt
    12361 caaatccttt taggatgtcg atggcttctt gcattatggg catatccgat atgcttccca
    12421 ttatgatggc tactttgctc atttgttttt attgttttta tttgaaaaat aaataataga
    12481 ttatataaaa tgtaaatgtt attagaaata gataaagtgc aattaataaa atgaaagaaa
    12541 aaaagctaat tattctttct cctaaatatt catttttatc agagaaaata ttccaatttt
    12601 tttttacaag attttctgaa tcagtaagat tcaataaatt tataataaaa tctaatatat
    12661 ccatttatgc aatcactctt attgtattct ttacatcctc tgcaattttt ctcgctacag
    12721 taatgtcttc attcactatg gtaacgtgtc ccatttttct aaaaggacgt gtttgttttt
    12781 ttccatagat gtgaggtgta acaccgttcc atcctaaaat agtttctatg ttttcgtaaa
    12841 ttacatctcc cgaaaaacct tcagcaccta ctagatttac cataattcct gcgactttgc
    12901 tgtcggtgtt tcctaaaggt aaatcgagaa cagcacgcaa atggttttcg aattgcgaag
    12961 tataactggc ttctatagaa taatgacctg aattgtgtgg gcgaggagcg acctcgttta
    13021 ccaatatttc atcgtcttgg gtttggaaca tttcgactgc caaaagtccc acgtgattga
    13081 atttttcgga aacatcaaga gctatttctc tagctttcaa ggctactttg tcgtcaattc
    13141 gagcgggaca aattacatat tccacttgat tggcttcagg atgaaattcc atttctacca
    13201 caggataagt tttgatttcg cccgaaggat tgcgacaaac gataaccgcc aattcatttt
    13261 tgaaaggaac catttcttcg gcaatgcatt ctacgttcgg taaattatct aaatcttgca
    13321 gtgttcgaac aatttttaca ccattgccgt cataaccaaa ttcggtgcat ttccatacga
    13381 atggtaattt tagattgctg aattcagatt tcagattttc taaattagaa aatttttgaa
    13441 atggagcggt cggaatatta tgttgggagt aaaattcttt ttgaacacct ttgttctgaa
    13501 tcaaacggag cgtttttggt gatggatata caggcaaacc ttcgttttct aatttttcca
    13561 acgcttctaa attgaccaat tcaatttcaa aagtcaaaat atcgactttt ttaccaaaat
    13621 tataaacggt gtcaaaatcc atcaaatcgc ctttgaagaa ttgattgcag gcgattttgc
    13681 aaggcgcttc atcacttgga tccaaaatgt aggtttgaat atcgaatttt ctggtgtcag
    13741 acaaaagcat tttgcccaat tgcccgccac ctaatatgcc taatttaaaa tcggaagaaa
    13801 aataattcat tgagttgtag tttgatgtgg caaagatact ttataatgac cgaataggaa
    13861 aatgctattt tattttcttc aagatttact ttccaacgac tttctagaaa gtagaatgat
    13921 ttatttggtt tttttaattt tgattgaaaa gtagaaatca attgtcaatt ctcaatttcg
    13981 aaatccctat atttgcaaat tatttttaaa acgaaaaaag tgatacaact acacgataaa
    14041 caatttgttc catttatttc tgctcaggaa atagatttcg ctatagcaaa aattgctgct
    14101 ttagttgagg atgattttgc cgacgaagtt ccagtttttg taggcgtttt gaacggtgct
    14161 tttatggtag tttctgattt tatgaaacat tataaatcac cttgcgaagt aagtttcatt
    14221 aaaatggcat cctacgaagg aactacatca accaatgaag ttaagcaatt aataggttta
    14281 gaccgcgatt tgtctggaag aagcgtagtt attatagaag atattgtgga tacaggcaat
    14341 actttggtag ctctaaaaga cttgttcaaa aagcaaaatg taaagcattt caaagtagca
    14401 acccttttct tcaagcccga agctttaaaa aatgatatta aaattgatta tgttggtatc
    14461 agaataccta ataaatttat agtaggattc ggtttagact atgatggttt gggtagaaat
    14521 ttaccagaag tttatcaatt gaagtaataa tatagaaaaa aaacaataac ataactcaca
    14581 atttagaatt ttaaaatgat taacatagtt ttatttggga aaccaggagc aggaaaagga
    14641 actcaggccg agtttttaaa aggaaaatac aatttagtac atctttcaac aggagatatt
    14701 tttagattta acatcaaaaa tgaaaccgaa ttgggtaaat tagccagaac ttatatggac
    14761 aaaggcgatt tagttccaga tgaagtaacc atcaaaatgt tgcaaagcga agtggaaaaa
    14821 aatcaagatg cagcaggatt tttatttgat ggttttccaa gaacaattgc tcaagccgaa
    14881 gctttagatg cttttttggc taccaaaaac gacaaaatta ccgcaacagt tgcattagaa
    14941 gccaacgacg aaattttagt agctcgtttg ctagaaagag gcaaaactag tggaagacct
    15001 gatgatcaag atgaagaaaa aattagaaat cgttaccaag aatacaacga aaaaacagct
    15061 cctttgatgg actattacac taagcagaat aaattttatg ctgtagatgg aattggttct
    15121 attgccgata ttacggagcg attaagtaaa gtgatcgata atttataaaa agtttatagt
    15181 ttatagttta tagttggtaa tatccaatgt atcagcaaaa cataaacaac aaacaataaa
    15241 caaaccaatg gaaattttaa tcattctttt tttaatcatc ctcaacggcg ttttctcgat
    15301 gtcggaaatc gcattgattt cggcgcgaaa aaatcgacta gaaacggctg caaaaaaagg
    15361 aaataaaaat gcccaagttg cattagattt agccaattct cccaataaat ttttatccac
    15421 agttcaaata ggaatcacgc taataggcat tcttacgggt atttattcag gagataaaat
    15481 tacacacgat gttcaagatt ttgttggcgg ttttgaagtt ttgaaaccct ttgcagcttc
    15541 acttggagtc ggaattgtag tggttgtttt aacttttttc tctttggttt taggagagtt
    15601 attgcctaaa agaattggat taaatcaccc agaagctatt gcaaaagcag tagctctacc
    15661 aatgaaagta atttcgattg taaccgctcc atttatatgg ttgttaaccc attcgaccga
    15721 gtttttattg aacgttttgc atataaaacc tactgctgat ggcaaagtta ccgaagaaga
    15781 aattaaagca attatcaaag aaggaactga aggcggcgaa gttcaagaaa ttgaacaaga
    15841 catcgtggaa cgtgtttttc atattggtga ccgcaagata aattcattga tgacccacag
    15901 aaaatcagtg gttattctgc cggtgcatgc aaacaaagaa caggttaagg aatttatgct
    15961 taaagaattg catagtattt accccgtttt tggcgaaaat catgatgata ttctgggtgt
    16021 tgtcaatttg aaaaatatat ttgcaactat agacaaagcc gattttaatt tgggcgacat
    16081 tatgaccgaa gcgcctttta tgatggaata taccacggca tacaaggcat tagagcaatt
    16141 caaagactcg ggcgttcact atgcttttgt ttcggatgaa tatggttttt ttcaaggaat
    16201 tatcactttg aatgatattt tggaagcttt agtaggagat gcatccgatt tttataaaga
    16261 cgattttcag ttaatcgaaa gagaagatgg aagttggtta gttgatggac attattcctt
    16321 acacgatttc ttgacttatt tcgaattaga tgatctaatt agtgattatg aagtaaccac
    16381 tgtaagcgga ttaataatga ctgaactttc tcatatacca aaagaaggag aaaaattagt
    16441 ttggcaaaaa tttgaattag aagtcctaga tatggatggg gtgaagattg ataaagtaat
    16501 ggtaaaagcc ataaaataaa aaggttttgg gtcttgggtt ctgggtttta gtttgcccaa
    16561 tacctagaac ccaagaccaa aaacctaaaa aaaatgacag aggggaactt cgtagattac
    16621 gtaaaaatat ttgtttcttc cggtaaaggc ggaaaaggat ctacgcattt gcatagagaa
    16681 aagtttatcc agtatggagg tcctgatgga ggagatggag gtcgtggagg acacgttgtt
    16741 ttggttggaa acaaaggact gtggacctta tttcatttga aatttgcccg tcacgtcaag
    16801 gcaggtcatg gtggcgatgg aggaggagac agaagtacgg gtgccgatgg agaagacaaa
    16861 tacatcgaag tgccattagg aactgttgta aaagacaaag aaaccggaga aacattattc
    16921 gaaattaccg aagacggtga gaaaaagatt ctgtgtcaag gaggaaaagg aggtttagga
    16981 aactggcatt ttcgtagttc tactaatcaa acaccaagat atgctcagcc cggtttgccc
    17041 ggtttagaaa tggacgtgat tttagaactt aaagttttgg ctgacgtagg attagtaggt
    17101 ttcccaaatg cagggaaatc aactttattg tcggttttga cttcagcaaa acccaaaatt
    17161 gccgattatc cgtttaccac tttaaaaccc aatttgggga ttgtagctta ccgagatttt
    17221 caatcatttg taatcgccga tatccctgga attattgaag gtgcagccga aggaaaaggg
    17281 ttgggacatt atttcttgag acatatcgag cgtaattcga ctttgctatt tttggttcct
    17341 gttgatactc caaatatcaa agaagaatac gatattttgg tcaacgaatt gacaaaatac
    17401 aatcccgaaa tgttggataa agagcgtttg ttggtgattt caaaatgtga tatgctagac
    17461 gacgaactca aagccgaatt gaaaatacaa ttggacaaag agtttaaaga cattccatat
    17521 atgtttattt cctcagttgc tcaacaaggt ttacaagatt tgaaagacaa gctttggaaa
    17581 atgttgaatg attaaaggtt tatctttttt atataataag gtgttctaga aatagggtac
    17641 ctttttttat tttaaaaagc tgttttgtct taaatttttt ggaatatttc ttcaaatatt
    17701 ttatcaaatc cttaaacatc attacttttg cgatactatt tttagtgaat attctggttt
    17761 tgaataaaaa aacatttttt tataggttca gccagttttc aagtaaccct ttataaatta
    17821 aaaaatgaaa aaatttattt ttgccttaat cgctggaata ttgctgttac caaattcagt
    17881 taaagccgat gagggaatgt ggtttttaat gtttatcgaa cgtttgaacc accgtgatat
    17941 ggaaaaaatg ggattgcaat tgacatccga agaaatttac agcattaacc acagtagttt
    18001 gaaagacgct atagtacaat ttaatggcgg atgtacagcc gaaatcgttt cgaaagaagg
    18061 tttggttttg accaatcatc attgcggata tagtgcaatt gcagaacttt ctacagccga
    18121 acaaaactat ttgaaagacg gtttttgggc aaaagacaaa agtgcagaaa tgaaacccaa
    18181 gtccttatac gttcgttttt tcgttcgaat ggatgatgtt tcgaaaagaa ttttgttaaa
    18241 agtcaatgat aaaatgtctg aaacagatcg aaataaagta atccaacaag agatagcgct
    18301 gattgaaaaa gaaaacagcg aaggtggaaa atacaccgtt tcggttcgtc ctttctttca
    18361 aggaaatgaa tactattatt ttgtttacca agattataaa gatgtgcgat tagtaggaac
    18421 acctccagaa agcatcggaa agtttggtgg cgacactgac aattgggaat ggccacgtca
    18481 tactggcgat ttttcgatgt ttagagtgta tgccgataaa aacggaaatc cagccgaata
    18541 ttcaaaagac aatttgcctt tgaaacccaa acattattta ccaataagtt tgaaaggtgt
    18601 tgaagaaaat gatttcgcaa tgattttggg ttatccaggc agaactaatc gttggatgcc
    18661 agccaatgga attaatcaaa atgtgaattt tgcatatcct gcttgggttg aaggagcaaa
    18721 aatgggaatg gacaggatga aagtttatat ggataaggac gaaacagtaa gtttgcaata
    18781 tgcctcaaaa tatgcttcga ctgccaatta ttggaaaaat cgtcaaggaa tgattgatgc
    18841 tttgactaaa tcaggaacgg tagaaactaa aactatagag gaatctaaat ttaatgattg
    18901 ggccaataaa tcggagaata aagctaaata tggtcaagtg gtggcgacaa taaatgacta
    18961 ttatgccaaa accaatttga aagcacgtca cgataattac ctttcgcaat tgatgcgaac
    19021 agctacttat ggctctgctc cagctaattt aggaaactca ttactgtatt attttaatga
    19081 aaatgaagcc aaaagagctg aggtacttcc gaaaattaat gctttgattg agtcggttta
    19141 cggaaagttt tatgctccat tagagaaaga tgttttgata gctcaactga atttatacac
    19201 ttcgaaaggg gctgaattcg gactttcgcc aatgatttca aaattgaaag aagacaataa
    19261 aggcgatttt gccacagtgg ttaatgaggc agttttcaaa agtattttta catcaaaaga
    19321 aactgttttg gcttttttga ataacccaaa gccagaggca attagtgctg atccacttta
    19381 tgtgctctcc aatgatttac ttacaaaagt aagagcaaaa tcagaagatc agcagcaaat
    19441 ggatgataat ttcgagaaag cctaccgcaa cttggtagaa ggtttgcgag aatctaaaat
    19501 aaattctatt caatatcctg atgccaattc tactttgaga ttgtcatacg gaaaagtaaa
    19561 tgctttgcct aaagatccac gcaacgatgc caaggttaat aattatacaa ctatgacagg
    19621 attggtcaaa aagtataaag caggagacga agaatttgat ttgccagctc gattattaga
    19681 attaaataag gcaaaagatt ttgggaaata tgccgataaa gatggatata tgccaataaa
    19741 tttcttgacc gataatgaca ttacaggagg aaattcaggt tctccagtac tcaatggaaa
    19801 aggggaattg ataggaattg cctttgatgg aaacattgaa gctatggctg gagatgttat
    19861 ttttgacaaa aaattacaaa gaaccatcag tgttgatatt cggtatgtgc tttggctgat
    19921 agacaaatat gcaggtgcaa aacacatcgt tgacgaaatg accattgcgg gagaaaactt
    19981 gaaataaagt taaaaccaac atatatttta aactgccctc aaaaagtaag ctgctatttg
    20041 agggcatttt tttatggaaa ttaaattaat aagaaataag tttttttagt gggaatttaa
    20101 tcgcttttgg gttttttatc ttcctcggag aaagagtatt tatgagtgtt tttctccaaa
    20161 caccactcta atcccgataa gtatcaaaca aaaactgcaa ggtttcattg atagtattag
    20221 ctttcatttt tgggtaaata actacagaat tcaaccagtt ttctataatt aaatcaaaat
    20281 cattacccac ttcatatacc acaggaacgc ccattttctt taaggcagcc gcattgcatt
    20341 cttgttcata atgatctcga ataggaatgc tcaatacttt ttttcgaagg aataaagctt
    20401 cggcaggagt ttcaaatcca ccacccgtaa ttattccgtg acaattaatt aagctctcgt
    20461 taaacaactt ttgattgaca gggaaataag taatgtttcc cactttatgt ttggtttgaa
    20521 catcattcaa aaaccaatga aattccacat ccgagagttt atgaaacgct ttttccaaac
    20581 aatctttttg gaatgaaggc aaataaaccg tgatgtgttt taaatctttg ggttcggctt
    20641 gtgtgatttc atctttgata acaggcggat gaataaagga atcataattt tcgaaatgca
    20701 agccgatgtg tttgggagaa ggcgcataat gtttgagaat catttctccc attaggctac
    20761 gtttttcggg tcttggtgtg tgttctgaaa caaaactggc ttgatgacca aattgcaccg
    20821 aatgcacttt ttgcattttg cacgccaaag cagtaaccga atcaaagtcg ttgataatta
    20881 tatcataatt tttcaaaggc aaggcagtag catctttgta gatttggtga gggcgaatgt
    20941 tttttacaat atcccaataa ttcaaaccgc cacatttgct gtaatgcaag ctgcaacccg
    21001 cacttctgaa ttttatgggc aaattaatat ccaaactcgc attgttgccg ctcaaaaaga
    21061 aatctacttc accaaattgc tgtaaataag gatacaattg agtcgctctg ctaatatgtc
    21121 cgttgcctgt ggcttgtatg gcataaaata ttttcatatc atttagattt taccgcaaat
    21181 tcgcaaactt tttttcctgt ttttgttctt ttaatttgcg aatttgcggt ttacattttt
    21241 ataaacacta aacttttagg ataaaatttt ggaaacaata tcattataaa ccacttcttc
    21301 ttttggttcc aattgcgaga aatctgattt tttatactga taaattttcc atttttcttt
    21361 cttgtattcc aatgcggtca ggttttcgat ccaatcacca ctgttcaaat acaaaacttc
    21421 tccgtgctgg ttttttacca cttttttttg tggcatatga atgtggccac aaacgacata
    21481 gctgtatttt ttttcgatgg caatttcggc agcggtagtt tcaaaat
//
LOCUS       DAOSNN010000014        21144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_2502_length_21144_cov_6.327422, whole genome shotgun sequence.
ACCESSION   DAOSNN010000014 DAOSNN010000000
VERSION     DAOSNN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 21144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21144
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2502_length_21144_cov_6.327422"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLQ70_01535"
     CDS             <1..203
                     /locus_tag="PLQ70_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE33326.1"
                     /translation="IAGKHPTIPTGEALTAVSSVSFLGFLMGPPVIGHISELFGLQFS
                     FAFIGMFGILIAFLVKRVKAME"
     gene            312..1538
                     /gene="hflX"
                     /locus_tag="PLQ70_01540"
     CDS             312..1538
                     /gene="hflX"
                     /locus_tag="PLQ70_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQE33327.1"
                     /translation="MLEKEVINFEKTAIVGIVTQNQSEDKLNEYLDELEFLTFTAGGE
                     VVKRFWQKMDKPNPKTFLGTGKIDEINLFVKENGISTLIFDDELTPSQQKNISKIIDC
                     KILDRTNLILDIFAQRAETSYARTQVELAQCIYLLPRLSGLWTHLERQKGGIGMRGPG
                     ETEIETDRRIVRDRISLLKEKIKAIDKQMGTQRSNRGAMVRVALVGYTNVGKSTLMNA
                     VGKSDVFVENKLFATLDTTVRKVVIKNLPFLLSDTVGFIRKLPTQLVDSFKSTLDEVR
                     EADLLLHVVDISHPDFEDHIESVNQTLADIKAKDKPVLMVFNKIDAYKHLTIDKDDLM
                     TEKTPRHYTLEEWKTTWMHRVGEENALFISAANKENFEEFRERVYEAVRQIHITRFPY
                     NKFLYPDYKDATEKEE"
     gene            complement(1685..1930)
                     /locus_tag="PLQ70_01545"
     CDS             complement(1685..1930)
                     /locus_tag="PLQ70_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33328.1"
                     /translation="MDVVKFKITPKTIKVEVRNSNNYVFDFNTAVEMQKEDRDVLLKL
                     YNALDLLFRKENVIEVKNYISPNQTNILDQINEVGEL"
     gene            complement(2084..5449)
                     /gene="mfd"
                     /locus_tag="PLQ70_01550"
     CDS             complement(2084..5449)
                     /gene="mfd"
                     /locus_tag="PLQ70_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQE33329.1"
                     /translation="MSKKNLYSIYDNSPKVKQIVDSLQQANTKIQLNGLLGSSLSFVV
                     QSLFKKAEKPFLLILNDKEEAAYYLNDLEQMIGEEDVLFYPGSYRRPYQIEDTDNANV
                     LLRAEVLNRINSRKKPAIIVTYPEALFEKVVTRKELDKNTLKVAVGDQISIDFINEVL
                     FEYEFKRVDFITEPGEFSVRGGIVDVFSFSNDNPYRIEFFGNEVDSIRSFDVETQLSI
                     EKQKKITIIPNVENKFFQENRESFLEYINNQTVLFIQDAEGLFTKLDRLFAKATEIFD
                     NLKATVNHVAPNQLFLNQEQFIKKSLDFSVVELSSKAIYKTKKTFDFYIKPQPSFNKQ
                     FDLLLNNLNDNHFNGYKNYLFCSNEAQAKRFHDIFESLDEENSENIRKQYNTIVLPIY
                     QGFIDEENQLACYTDHQIFERYHKFSIKSANSKKQNITLKELTTLTVGDYVTHIDHGI
                     GKFGGLQKIQVEGKTQEAIKLVYADNDIVYVSIHSLYKISKYNGKDGTPPKIYKLGSN
                     AWKILKQKTKARVKHIAFNLIQLYAKRRLEKGFQFAPDSYLQNELESSFIYEDTPDQT
                     KSTAEVKADMESDRPMDRLVCGDVGFGKTEVAIRAAFKAVDNSKQVAVLVPTTILAYQ
                     HYRTFSERLKDMPVSVGYLNRFRTAKQKAETLKQLAEGKLDIVIGTHQLVNKNVVFKD
                     LGLLIVDEEQKFGVNVKDKLKTIAANVDTLTLTATPIPRTLQFSLMAARDLSVITTPP
                     PNRYPIETNVVGFSEELIRDAISYEIQRNGQVFFINNRIENIKEVAGMIQRLVPDARV
                     GIGHGQMDGAKLEELMLAFMNGEFDVLVATTIIESGLDVPNANTIFINNANNFGLSDL
                     HQMRGRVGRSNKKAFCYFICPPYSAMTDDARKRIQALEQFSELGSGFNIAMKDLEIRG
                     AGDLLGGEQSGFINEIGFDTYQKIMNEAIEELKENEFKELYENEDSGVEKEYVKDLQI
                     DTDFELLFSDEYINNVTERLSLYNELGDVKNEEELVVFQNKLIDRFGPMPPRALALMN
                     SIRIKWIASKVGIEKLVMKKGKMIGYFVSDQQSDYYTSNRFHKVIQFVQKNSNICVMK
                     EKQTPAGLRLLLTFDNAKTTRRCLELMEMLGGE"
     gene            complement(5442..6050)
                     /locus_tag="PLQ70_01555"
     CDS             complement(5442..6050)
                     /locus_tag="PLQ70_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33330.1"
                     /translation="MKLKYIVLFLFIFALFSCKNEDEKRQVESLKDIKKKELIFSIIE
                     KAWVFNAKPINTTAQINTNTWKEWRSFLEELDQKPKKTIGAFQKKAAELSKKVMALND
                     FTPVEYNNPQMKSRIAILITKVRMLDLYIHLTDIPEQKVIALIPEINTQLLALQNQMD
                     KISQKSKIPLEEGESELLQMIKDTARAIPNSPQFDPNTPRVE"
     gene            6274..7227
                     /locus_tag="PLQ70_01560"
     CDS             6274..7227
                     /locus_tag="PLQ70_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HQE33331.1"
                     /translation="MNPKILIIGACGQIGTELTHRLRELYGIENVVASDIRKLNNDIV
                     NSGIFEVINALDFNQIEHLVEVHHITDIYLMAALLSATAEKNPAFAWDLNMNSLFHVL
                     NLAKAKKIKKVFWPSSIAVFGPTTPRENTPQYTIMEPSTVYGISKQSGERWCEYYHTH
                     FGVDVRSVRYPGLISWSTPPGGGTTDYAVDIYHKALSDGKYECFLSSETKMPMMYMDD
                     AIDATIRIMQAPAENIKIRSSYNLSAMSFTPTEIAAEIKKHIPDFTISYEPDFRQIIA
                     DSWPASIDDSRARADWNWHHEFDLESMTVDMLEHLKQIDYH"
     gene            complement(7224..8366)
                     /locus_tag="PLQ70_01565"
     CDS             complement(7224..8366)
                     /locus_tag="PLQ70_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018942311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HQE33332.1"
                     /translation="MKSFDVAIIGSGPSGASAAFELAKSGLSTVIIEKEILPRYKTCG
                     GGLVFRGRKNMPFDISSVVEKEFFEVDTYFANTNLKFTTNRDQPIVSMIMRDAFDHLI
                     VEKAKENGVTLLQNHKVLAISFGEIQTIHTSEGDVQAKFIIAGDGALSPVAKITGWQE
                     TRNIIPALEYEVEVPAADFERLSKNVRFDVDVIPLGYGWCFPKKNHLSIGVGVFVKTK
                     QKIDLKKYYADYLKTLGITEIINEEAHGFVIPVSPRTDTFVQKNVFLIGDAAGFADPI
                     VAEGISNAILSGVLAAQSIIESKLDGVLASKLYHEKLENSILPEIRTGVVLSKFFYEK
                     RALRNLFLKKYGQLLANAMTEVFMGERTYPKDYKATIRRKIKEVIF"
     gene            complement(8450..10336)
                     /locus_tag="PLQ70_01570"
     CDS             complement(8450..10336)
                     /locus_tag="PLQ70_01570"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQE33333.1"
                     /translation="MKKILLISVFLLSFANGFAQSSEYWQQHVDYKMDVVMNVKNDQY
                     KGKQKLVYTNNSPDTLKRVYYHLFNNAFQPGSEMDARLQSIKDPDARMVNKVKHGDTE
                     IKESRIKTLKPDEIGYLNISNFKQDGITSTAKTVGTILEVTLAKPILPHSKTTFTLDF
                     DGQVPVQIRRSGRNNAEGVELSMAQWYPKIAEFDFEGWHADPYIGREFHGVWGDFDVK
                     ISIDKEYVLGGSGYLQNKNEIGHGYQDAGISVVYPKNTKTLTWHFYAPMVHDFTWAAD
                     KNYLHDIVKGPNNVDLHFLYKNNPEIIENWKALQPKMVQIMKFYNNFVGNYPYKQYSF
                     IQGGDGGMEYAMCTLMLGNGKFEGLLGTATHELGHSWFQHILASNESKHPWMDEGFTS
                     YIEDLALNEIAEKKTENPFLPTYKAYYKLVESGKEQPQTTHGDRYDENRPYSISSYYK
                     GEIFLAQLEYLIGKENVAKTLKKYFQDFKFKHPTPNDIKRTAERVSGANLDWYLTDWT
                     QTTNTIDYGIKEVRNDGNFEGLNTIIALERIGRMPMPIDILVEYTDGTKESFYIPLRM
                     MSFEKENPNPAIKRTVLSDWTWANPTYTFWVDSNTKTIKKITIDPSGLMADVKQADNT
                     YEIK"
     gene            complement(10401..12020)
                     /locus_tag="PLQ70_01575"
     CDS             complement(10401..12020)
                     /locus_tag="PLQ70_01575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HQE33334.1"
                     /translation="MINFKNSLLASFAVLMLAGCGASKNTSYSNLTMIQAPVNVQRKT
                     ALKEADLNRWSHLDIIKDTIPGMSVDRAYAELLKDKKGKKVIVGIVDSGVDIDHEDLK
                     AVVWTNKKEIAGNGIDDDKNGYIDDIHGWNFLGDITKENIEYERIVANKNLVDEATYQ
                     EAKAVNDKNIAKSTTGKARLEEMIADATNADAVLTKYFGKSTYTKEEVNAISSQDAEI
                     NKSKMMIQRVNSFEMTVPEFKTAVQKELDGYIKVVNGDNLKTNYRKVLGDNPEDITDT
                     KYGNNNVMGPDKEEILHGTHVAGIVAQVRNNGIGGDGIANNVEILTVRAVPDGDEYDK
                     DIALGIRYAVDNGAKVINGSFGKSFSPHKQWVYDAIKYAESKDVLIVHAAGNDAKDID
                     VANNYPNDSDDKINEFADNMITIGALNYEYGNKIVARFSNFGKLNVDVFAPGVKIYAS
                     TPNNTYKLLQGTSMASPNVAGVAALIRSYYPKLSAKQVKHILMDSGVAITTDVIVGGK
                     PNDVRPFSTLSKSGKMVNAYNALLMADKMSK"
     gene            complement(12096..12956)
                     /locus_tag="PLQ70_01580"
     CDS             complement(12096..12956)
                     /locus_tag="PLQ70_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQE33335.1"
                     /translation="MKLYPIETGNFKLDGGAMFGVVPKTIWNKTNPADANNLIDLAAR
                     CLLIEDGNRLILIDNGMGNKQSEKFFGYYFLWGNHSLDNSLAKYGFHRDDITDVFLTH
                     LHFDHCGGSVNWNKDKTGYEVAFKNAKFWTNENHWKWATKPNLREKASFLSENILPIQ
                     ESGQLHFLELPENDFGFSEELNFGIHYVNGHTEKQMIPQIKYQDKTICFMADLIPTVG
                     HLPLPYVMGYDTRPLLTMPEKSKFLNAAAENNYYLFLEHDAHNEIITVEQTERGIRLK
                     ETYSCDKIFK"
     gene            13126..13455
                     /locus_tag="PLQ70_01585"
     CDS             13126..13455
                     /locus_tag="PLQ70_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HQE33336.1"
                     /translation="MIKVSDTAKKKIIDLMKEDGFDAAVDYVRVGVKSGGCSGLSYDL
                     KFDKNKGDDDKIFVDNDITIAVEKKSFLYLAGTILEFSGGLNGKGFVFNNPNASRTCG
                     CGESFSL"
     gene            13474..14922
                     /gene="sufB"
                     /locus_tag="PLQ70_01590"
     CDS             13474..14922
                     /gene="sufB"
                     /locus_tag="PLQ70_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HQE33337.1"
                     /translation="MSKYTEDHLRVELETKEYEYGFYTDLESDTFPIGLNEDIVRAIS
                     QRKEEPEWMTDWRIEAFRAWEKMIEPEWANVHYNKPDFQAISYYSAPKAVDPNKTLDD
                     VDPELLEMYKKLGISIDEQKKMNNVAMDIVVDSVSVATTFKKTLGEKGIIFMSISEAI
                     KEHPELVKKYIGTVVPQKDNFYAALNSAVFSDGSFCYIPKGVHCPMELSTYFRINQAG
                     TGQFERTLLIADEGSYVSYLEGCTAPTRDENQLHAAVVELIALDGAEIKYSTVQNWYP
                     GNKEGKGGVFNFVTKRGLCEKDAKISWTQVETGSAVTWKYPSCILKGDNSVGEFYSIA
                     VTNNFQQADTGTKMIHLGKNTKSTIISKGISAGKSQNSYRGLVKIGPNADNARNFSQC
                     DSLLMGNNCGAHTFPYIESKNTSAKIEHEATTSKIGEDQVFYCNQRGIPTEKAIALIV
                     NGFSKDVLNKLPMEFAVEAQKLLEISLEGSVG"
     gene            15012..15764
                     /gene="sufC"
                     /locus_tag="PLQ70_01595"
     CDS             15012..15764
                     /gene="sufC"
                     /locus_tag="PLQ70_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HQE33338.1"
                     /translation="MLSIKNLHASIGDKEILKGINLEVKAGEVHAIMGPNGAGKSTLS
                     AIIAGNENYEVTEGEILLDNEDISELAPEERAHKGVFLSFQYPVEIPGVSVTNFMRTA
                     INETRKANKLEEMPANEMLKLIREKSELLEIDRKFLSRSLNEGFSGGEKKRNEIFQMA
                     MLEPKLAILDETDSGLDIDALRIVANGVNKLKSDKNSIVVITHYQRLLDYIVPDFVHV
                     LYNGKIVKSGTKELAYELEEKGYDWIKQEQEA"
     gene            15834..17150
                     /gene="sufD"
                     /locus_tag="PLQ70_01600"
     CDS             15834..17150
                     /gene="sufD"
                     /locus_tag="PLQ70_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HQE33339.1"
                     /translation="MELKEKLISSFMAFEEQIDVHSELHDLRTAAIKNFENKGFPTKK
                     EESWKYTSLNAILKNDFTVFPKSETSIEFKDVKKYFIHEIDTYKIVFIDGVFSSNLSS
                     TTHQGLDVCLMSSALNKPKYKMVIDAYFNQIASKDESLTSLNTAFANEGAYINIPKKT
                     VVQKPIEIMYFSTGNEAALLVQPRNLVIVGENSHVQIIERHQSLNENPVLTNSVTEIF
                     AQKRAIVDFYKIQNDHLGANLIDNTYVSQKQESHVSVQTFSFGGNLTRNNLNFYHFGE
                     RLTSTLNGITIIGDKQHVDHYTLVHHAAPNCESFQDYKGIFSDNSTGVFNGKVYVEKE
                     AQKTNAFQKSNNILLGEKATINAKPQLEIFADDVKCSHGCTVGQLDETAMFYMQQRGI
                     PKKEAKALLMYAFSNAVIENIKIPELKQRITKIIALKLGVKIGFDL"
     gene            complement(17253..18599)
                     /locus_tag="PLQ70_01605"
     CDS             complement(17253..18599)
                     /locus_tag="PLQ70_01605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQE33340.1"
                     /translation="MKKIFKFLGLAFLLFLIYLGYTTYPKLDLISGFSAKSMASGHFI
                     DHRSQEMIEKGDNDIKMITLSKNQIDETGKFATASVYGLKERKAIYREGLGATLINDN
                     FDISKPYEVPKRTKLNNNLPFPYGNNEPKDTIFDNIDYDKLNAAVANAFDAKGQKIKR
                     TRSVIVIYKDKIIAEKYDTGFDKNSKILGWSMTKSITATMYGILQKQGKLNINKLAPI
                     QEWANDERKNITINDLLHMNSGLEWEEDYTKICDATNMLFLAEDMTRVQLEKPAKFKP
                     NTHWNYSSGTTNLLSGILRKQFKTHQEYLNFWYSALIDKIGMNSMIIETDMAGNYVGS
                     SYSWATTRDWAKFGLLYLHKGNWNGEQLFDESWAKYVATPTNGSNGDYGGHFWLNAGG
                     FYPDAPRDLYSANGYQGQRVFILPSQDLVIVRMGLSEEVKFDFNGLLKGIVGSLKK"
     gene            18750..19964
                     /locus_tag="PLQ70_01610"
     CDS             18750..19964
                     /locus_tag="PLQ70_01610"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HQE33341.1"
                     /translation="MLDINKVRADFPILSRTVNGKPLVYFDNGATSQKPQIVIDAIAK
                     YYQEINANIHRGVHTLSQLATDAYEISRSKIQNHINAKFAHEVIFTSGTTHGINAIAN
                     GFISILKSGDEVLVSALEHHSNIVPWQMLCEKTGATLKVIPMDENGELIMAEYDKLLS
                     DKTKIVTVNHISNALGTVNPIKYMIDKAHEFGAAILIDGAQAVPHLKPDVQALDCDFY
                     VFSGHKMCGPTGVGILYGKEAWLNKLPPYQGGGEMIKEVTFEKTTYADLPHKFEAGTP
                     DIAGGIVLGTAVDYLNSVGFDNIQKQELELLEYATKQLLEIEGLKIYGTAKEKTSVVS
                     FNIEGIHPYDIGTIVDKLGIAVRTGHHCAQPIMNFFNIPGTIRASFSFYNTKEEIDVM
                     VEAVKKAQMMLS"
     gene            19976..20410
                     /locus_tag="PLQ70_01615"
     CDS             19976..20410
                     /locus_tag="PLQ70_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33342.1"
                     /translation="MKLYAMIFLSLFLGKSCTSQTKNDLKTAVLEYTANTRGFYQKIT
                     IQNQMVAVSKDRSGTEKAVETKISDADWKELISYFETINLDSLATLKAPTEKRFHDGA
                     AIANLEVSYKDKKYKTTSFDHGYPPEAIQKLVTKINSFAKKE"
     gene            20410..20829
                     /locus_tag="PLQ70_01620"
     CDS             20410..20829
                     /locus_tag="PLQ70_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HQE33343.1"
                     /translation="MSIKDIQEEIVDEFSMFDDWMQRYEYIIDLGKNLPLIQEEFKTD
                     DNIIKGCQSKVWLKGEQNDDKIVFTADSDAILTKGIIAILIRVFSNQKAADILNADMD
                     FIDQIGLKEHLSPTRANGLVSMIKNIKMYALAFDAKK"
     gene            20835..>21144
                     /locus_tag="PLQ70_01625"
     CDS             20835..>21144
                     /locus_tag="PLQ70_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HQE33344.1"
                     /translation="MEQTINTDQLGIDIVAKLKTIYDPEIPVDIYELGLIYDVMISTD
                     YEVKILMTLTSPNCPVAESLPREVEEKIKSIEHIKGAEVEITFDPPWNKDLMSEEAKL
                     E"
BASE COUNT         6655 a         3865 c         3826 g         6798 t
ORIGIN      
        1 gtattgcggg aaaacatcca accatcccaa ctggcgaagc tttgacagct gtttcgagtg
       61 tgagtttcct cgggttcttg atgggaccac ccgttattgg tcatatttcc gaattatttg
      121 gattgcaatt ttcattcgct ttcatcggaa tgttcggaat tttgattgcc tttttggtca
      181 aaagagtgaa ggcaatggaa tagatttcac aagacaaaca ctttaaaaaa agaaaaaaag
      241 gcagccacga atttcaatgc aatgttcata agtccctcgt aaatcattaa atttgcggct
      301 tattaaaagt tatgttagaa aaagaagtaa taaattttga aaaaacggcc attgttggta
      361 tcgttaccca aaaccaaagc gaagataaac tcaacgaata tctcgacgaa ttggagtttt
      421 tgacttttac ggccggcggt gaagtggtga aacgtttttg gcaaaaaatg gacaaaccca
      481 atccaaaaac atttttggga accggaaaaa tagacgaaat caatcttttt gtaaaagaaa
      541 atggcatttc gacacttatt tttgatgatg aattaacacc ttcacagcaa aaaaatattt
      601 ccaaaatcat agattgcaaa atcctcgaca gaactaatct tatcctagat atttttgctc
      661 aaagagccga aacttcttat gctagaaccc aagtagaatt ggcacaatgt atttatttat
      721 tgccaagatt atctggtttg tggacgcact tagaacgtca aaaaggggga atcgggatgc
      781 gtggaccggg agaaacagag attgaaaccg accgtcgtat cgttcgtgac cgaatttcgt
      841 tgttgaaaga aaaaataaaa gccattgaca aacaaatggg aacccaaaga agcaatcgtg
      901 gtgctatggt tcgagtagct ttagtaggtt ataccaatgt tggaaaatcg accctgatga
      961 atgctgttgg gaaaagcgat gtttttgtag agaacaaatt gtttgccacc ttggacacca
     1021 ccgtccgaaa agtggtgatt aagaatttac ctttcctgct ttcagacacc gttgggttta
     1081 ttcgaaaact gcctacacag ttagtagatt ctttcaaaag tacactggat gaagttcgcg
     1141 aagccgattt attattgcac gttgttgata tttcgcatcc tgattttgag gatcatatcg
     1201 agtctgtcaa ccaaactttg gccgacatca aggcgaaaga taaacccgtg ctgatggttt
     1261 tcaacaagat tgatgcctac aaacatttga ccattgacaa agatgatttg atgactgaaa
     1321 aaacgccaag acattatacg cttgaggaat ggaaaaccac ttggatgcac cgtgtaggcg
     1381 aagaaaacgc tttgtttatt tcggctgcca ataaggaaaa ttttgaggaa ttccgagaac
     1441 gagtttatga agccgtgaga caaattcaca tcacacgttt tccttacaac aagtttttgt
     1501 atcctgatta caaagatgct actgagaaag aagaataaaa aaatatcatt cccttttccg
     1561 caaaatctcc tactttaagg taatgatttt tggtttttaa ccaaggttat gattaatttt
     1621 tagtcaatca ttgacagaaa aacactttcg taaagtcgga gactttgcgg aaccgaacaa
     1681 ggttttacaa ctctccaacc tcattaattt ggtctaaaat attcgtttga ttgggtgaaa
     1741 tataattttt tacttcaatc acattttcct ttctaaacag taaatctaaa gcgttgtata
     1801 attttaataa tacatctcta tcttctttct gcatctcgac cgcagtatta aaatcaaata
     1861 cataattatt agaattacga acttctactt taatcgtttt aggcgtgatt ttaaatttaa
     1921 ctacatccat aaatttattc tattaattta ctccaatgct ccacaaagtc tccgactttg
     1981 aggtagtgat tctcggtttt taaccgacct catatttaat ttttatgtca gtcacagact
     2041 gacgaacaca ttcacaaagt cggaaacttt gcgaagctaa aaattattcc ccgcccagca
     2101 tctccatcaa ttccagacat cttcttgtgg ttttggcatt atcaaaagtc agtaataatc
     2161 ttaaaccagc aggtgtttgt ttttctttca ttacacaaat attgctgttt ttttgcacaa
     2221 actgaatcac tttatgaaaa cggttcgaag tataataatc cgattgttgg tcggacacaa
     2281 aatacccaat catctttcct tttttcatca ccaatttttc gattcctact ttcgatgcaa
     2341 tccatttaat gcggatgctg ttcattaaag ccagcgctct tggtggcatt ggtccaaaac
     2401 ggtcaatgag tttattttgg aaaacaacca actcttcttc gtttttcaca tcacctaatt
     2461 cgttgtacaa actcaaacgc tccgtaacat tattgatgta ttcatccgaa aacaacaact
     2521 caaaatcggt gtcaatttgc aggtctttga catattcttt ttcgacgccg ctatcttcgt
     2581 tttcatacaa ttccttgaat tcattttcct tcaattcttc gatggcttca ttcataattt
     2641 tttgataggt atcaaagcca atttcgttga tgaaaccact ttgctcgcct cccaataaat
     2701 ctccagctcc acgaatttcc aaatccttca tcgcaatgtt gaaaccgctt cccaattccg
     2761 agaattgttc caaagcctga attcgttttc ttgcatcatc cgtcatcgct gaatatggcg
     2821 gacaaataaa ataacaaaac gcttttttat tacttcgccc cacacgacca cgcatttgat
     2881 gcaaatcaga cagcccaaaa ttattggcat tattgatgaa aatcgtattt gcattcggaa
     2941 catccaatcc gctttcgatg atggttgtgg ctaccaaaac atcgaattcg ccgttcataa
     3001 acgccagcat caattcctct aatttagctc catccatttg tccgtgacca attcccactc
     3061 gggcatcggg aaccaaacgc tgaatcattc cggcaacttc cttgatgttt tctattcgat
     3121 tattgataaa gaaaacctgt ccgttacgct gaatttcata cgaaatcgca tcccgaatca
     3181 gttcttcgct aaaaccaact acattggttt cgataggata acgattgggc ggaggcgttg
     3241 tgattacgga caaatctcgt gccgccatca acgaaaactg taaagttctc ggaatgggcg
     3301 ttgcggtcaa ggtcagcgta tcgacattgg cagcgatggt tttgagtttg tcttttacgt
     3361 tgacaccgaa tttttgctcc tcgtcaacaa tcaataatcc gaggtctttg aaaactacat
     3421 ttttgttgac caattggtgg gttccaataa cgatatccag ttttccttcg gccaattgtt
     3481 taagggtttc ggctttttgt ttggcagtcc tgaaacggtt caaataaccc acagaaacgg
     3541 gcatatcttt caatcgttct gaaaaagttc tgtaatgttg gtaggccaaa atcgtggtag
     3601 gaaccagaac ggcaacttgt ttcgaattgt ccaccgcttt gaaagccgca cgaatggcga
     3661 cttcggtttt tccaaaacca acgtctccac aaaccaatcg atccatcggg cggtcacttt
     3721 ccatatcggc tttgacttct gcggttgatt tggtttggtc cggcgtatct tcgtaaatga
     3781 aggaactttc caattcgttt tgcaaataac tgtcgggcgc aaactggaat cctttttcca
     3841 atcgacgttt ggcgtacaat tgaatcaaat tgaacgcaat atgtttgact ctcgccttgg
     3901 ttttctgctt taaaatcttc caggcattcg aaccgagttt ataaatttta ggcggtgttc
     3961 cgtcttttcc gttgtatttc gaaattttat acagcgaatg aatgctcaca tacacaatgt
     4021 cattgtcggc ataaacgagc ttgatggctt cctgggtttt gccttccacc tgaatttttt
     4081 gcaggccgcc aaactttcca attccgtgat cgatgtgcgt tacataatca ccaaccgtca
     4141 aagtggtgag ttcttttaaa gtaatatttt gctttttcga attagcactt ttgatgctga
     4201 atttatggta acgctcaaaa atttggtgat cggtgtaaca ggccaattga ttttcttcgt
     4261 cgataaatcc ttgataaata ggcaagacaa tcgtgttgta ttgcttacgg atgttttcgg
     4321 agttttcttc atccaaagat tcaaaaatat cgtggaatcg tttggcttgg gcttcattgg
     4381 aacaaaacaa gtaatttttg taaccattga aatgattgtc gttcaaatta ttcaacaaca
     4441 aatcgaattg tttgttgaac gatggttgtg gtttgatata aaaatcgaat gttttcttcg
     4501 ttttataaat cgctttcgaa ctcaattcaa caaccgaaaa atcgagactt ttcttgatga
     4561 attgttcctg atttagaaac aattggttgg gtgccacgtg attgacagtc gcttttagat
     4621 tgtcgaaaat ctcggttgct ttcgcaaaaa gtcggtcgag tttggtaaac aaaccttcgg
     4681 catcttgaat aaacaatacc gtttgattgt tgatgtattc caggaaactt tcgcggtttt
     4741 cctgaaaaaa cttgttctcg acgttgggaa taatcgtgat tttcttttgc ttttcgattg
     4801 aaagctgagt ttccacgtca aagcttcgaa tgctatcgac ttcgttgcca aaaaattcta
     4861 ttcgatacgg attatcattc gaaaaagaaa atacatcgac aatgccgcca cgaacggaaa
     4921 attcgccggg ttctgtaata aaatcaaccc ttttgaattc atattcaaac aacacttcgt
     4981 tgatgaaatc aatggaaatc tggtcgccga cggccacttt caaagtattt ttatccagtt
     5041 cttttctagt gacgactttc tcgaaaagcg cttcgggata agtgacaata atagctggtt
     5101 ttttgcgaga attgatgcgg ttcaagactt cggcacgcaa taaaacattg gcattatcgg
     5161 tgtcttcaat ttggtacgga cgacgatagg aacctggata aaaaagtacg tcttcttcgc
     5221 caatcatttg ttctaaatcg ttcaaataat aggccgcttc ttctttgtca ttcaggatca
     5281 ataaaaacgg cttttctgct tttttaaaaa gcgattgcac cacaaacgaa agtgacgaac
     5341 ccagcaatcc attgagctga attttagtgt tggcttgttg caaactatcg acaatttgct
     5401 tgacttttgg cgaattgtcg taaattgaat atagattttt tttactcaac tcgaggcgta
     5461 tttgggtcaa actgtggtga atttggaatg gctcttgcgg tgtccttaat catttgcaat
     5521 aattcagact ctccttcttc gagcggaatt ttgctttttt gggaaatttt atccatttgg
     5581 ttttgcaaag ccaataattg agtgttgatt tcaggaatta aagcaatcac tttttgttcg
     5641 ggaatgtcgg tcaaatggat gtataaatct agcattcgca ctttggtaat taagatggca
     5701 atgcgacttt tcatctgggg attgttatat tctacaggag taaaatcatt caatgccatt
     5761 actttcttag aaagttcagc tgcttttttc tggaaagcac caatagtttt cttcggcttt
     5821 tggtctaatt cttccaaaaa cgaacgccat tctttccaag tattggtgtt aatttgggca
     5881 gtagtattaa taggcttagc attaaaaacc caagcttttt cgataatcga aaaaattaat
     5941 tctttctttt tgatgtcttt tagggattct acttgacgtt tttcgtcctc atttttacaa
     6001 gaaaataagg caaaaatgaa taaaaagaga actatatact ttaatttcat tagatacaat
     6061 ttgagtcgca aagttacgaa gtaaatttac aaatatgcat tcgaacattt acaataacaa
     6121 tctaatatct ttgattcctg tttcttgcat ttacagaata taaattaaga aattatacaa
     6181 gattaataag gctaattttc agaaaatcat gctttttatt agaaattttg atgttcaata
     6241 aaacagtata tttgtataag aaaaaccaac cagatgaatc ctaaaatact gattataggt
     6301 gcttgcgggc aaataggaac cgagttaaca catcgacttc gggaattata tggaattgaa
     6361 aatgtggtcg catcagatat tcgaaaattg aataatgata ttgtgaactc tggaattttt
     6421 gaagtgataa atgcacttga ttttaatcaa attgagcatt tggttgaggt gcatcacatt
     6481 actgatattt atttgatggc ggcattatta tcggcaacag ccgaaaaaaa ccctgctttt
     6541 gcttgggatt tgaatatgaa ttcattgttt catgttttga atttggccaa agccaaaaaa
     6601 ataaaaaaag tattctggcc atcgagtatt gctgtttttg gaccaacgac gccaagagaa
     6661 aacacaccac aatacaccat tatggaacct tcgaccgttt acggaattag caaacaatct
     6721 ggcgaaagat ggtgcgaata ttatcatact cattttggag ttgatgttcg aagtgtgcgt
     6781 tatccgggtt taatcagttg gtcaactcct cctggtggcg gaaccacaga ttatgcggtg
     6841 gatatttatc acaaagcttt gagtgatgga aaatatgagt gttttttgtc ttcagaaacc
     6901 aaaatgccca tgatgtatat ggacgatgct attgacgcaa ccatcagaat tatgcaagct
     6961 ccagccgaaa atattaaaat tcgttcttct tataatttgt cggccatgag ttttactcca
     7021 accgaaattg cggctgaaat aaaaaaacat attcctgatt ttaccatttc gtatgagcct
     7081 gattttcgtc aaataattgc tgatagttgg ccagctagta ttgatgattc tcgagctaga
     7141 gcagactgga actggcatca cgaatttgat ttagaaagta tgaccgtcga tatgttagaa
     7201 catttaaagc agattgatta tcactaaaaa ataacttcct tgatttttct gcggatggta
     7261 gctttataat ctttgggata ggttctttct cccataaaaa cttcggtcat tgcattggcc
     7321 aaaagctgac cgtatttttt aagaaaaagg ttgcgtaagg ctcttttttc gtagaaaaat
     7381 ttagataaaa caaccccagt tcgaatctct ggcaaaatgc tgttttccaa tttttcgtga
     7441 tacaatttcg atgctaaaac cccgtctagt ttactttcaa taatggattg tgctgccaaa
     7501 acgccgctta aaatagcatt cgaaattcct tcggcaacga ttggatcagc aaatccagca
     7561 gcatcgccaa ttaaaaacac attcttttga acaaaagtat cggttctggg agaaacagga
     7621 attacaaatc cgtgagcttc ttcatttata atttcggtga taccaagggt ttttagatag
     7681 tcggcatagt atttttttaa atctattttc tgcttggttt taacaaaaac accaactcca
     7741 atggataagt gatttttttt gggaaaacac cagccgtagc ccaatggaat aacatcaaca
     7801 tcgaaacgaa cattttttga taatcgttca aaatcggcag caggaacttc tacttcatat
     7861 tctaaagctg gaataatatt tcgggtttct tgccatcctg ttattttggc aaccggactt
     7921 aaggctccgt ctccagcgat gatgaatttt gcctgtacat cgccttcact cgtatgaatg
     7981 gtttgaattt cgccaaacga aatggctaaa actttatggt tttgaagaag agtaacgccg
     8041 ttttcttttg ctttttcgac tatcaaatgg tcaaaagcat cgcgcataat catacttaca
     8101 attggctggt ctctgttggt ggtaaatttt aaatttgtat tggcaaaata agtatctact
     8161 tcaaagaatt ctttttctac taccgaagaa atatcaaaag gcatattttt tctgccacga
     8221 aagaccaatc caccaccgca agttttatat ctgggtaaaa tttccttttc aattatcacg
     8281 gtcgaaagac cacttttggc taattcgaac gctgccgaag ctccagaagg accactccca
     8341 attatggcta catcaaatga tttcatacgt ttttttttca aaactaaagg aatagttgtg
     8401 aaaaacaaat ttttagtttc aaatagctgt tttatagtaa aagaaaattt tattttatct
     8461 cataggtatt atcagcttgc ttcacgtcgg ccattaatcc acttgggtcg attgttattt
     8521 ttttgatggt tttggtattt gaatctaccc aaaatgtgta agtcggattt gcccaagtcc
     8581 aatcacttaa aactgttctt tttattgctg gattcgggtt ttctttctca aaactcatca
     8641 tacgtagcgg aatataaaaa ctctcttttg tgccatcggt atattcaacc aaaatatcaa
     8701 ttggcattgg cattctacct attctttcca aggcaattat cgtatttaaa ccttcaaaat
     8761 tcccgtcatt tcttacttcc ttaattccat aatcaattgt gttggtggtt tgtgtccaat
     8821 cggttaaata ccaatcgagg ttggctcctg aaacacgctc ggcagttctt ttgatatcat
     8881 taggtgttgg atgtttgaac ttgaaatcct gaaaatattt tttcaaggtt ttggcaacat
     8941 tctcttttcc aataagatat tctaattgtg ccaagaaaat ttcgcctttg tagtatgaag
     9001 aaatactata gggtctgttc tcgtcatatc ggtcgccgtg agtggtttgc ggttgttctt
     9061 ttcccgattc aactaattta taataggctt tataggttgg taggaatggg ttttctgtct
     9121 ttttttcagc aatttcattt aatgctaaat cttcaatata ggaagtaaat ccttcgtcca
     9181 tccacgggtg tttgctttca tttgaggcta aaatgtgctg aaaccatgaa tgtcctaatt
     9241 cgtgagttgc agttcctaaa agtccttcaa atttaccgtt tccaagcatt aatgtacaca
     9301 tggcatattc cattccgcca tcgccacctt gaataaagga atattgttta taaggataat
     9361 taccaacaaa gttgttgtaa aacttcatta tttgaaccat tttgggttgc aaagctttcc
     9421 agttttcgat aatttctgga ttgtttttgt aaagaaaatg caaatcgaca ttgttgggac
     9481 ctttgacaat atcgtgaagg taatttttat cggcagccca agtaaagtcg tgaaccattg
     9541 gggcataaaa atgccaagtc aaggttttag tgttttttgg atatacaacc gatattcctg
     9601 catcttgata accgtgacct atttcgtttt tgttttgcaa ataacctgaa cctcctagaa
     9661 catattcttt gtcaatagaa atttttacat caaaatctcc ccaaactccg tgaaattctc
     9721 ttccaatgta gggatcggcg tgccaacctt cgaaatcaaa ttcggctatt tttggatacc
     9781 attgtgccat cgataattcg actccttcgg cattatttct gccagaacga cgtatttgaa
     9841 ctggaacttg cccatcaaaa tcgagtgtga acgtagtttt cgaatgaggt aaaatgggtt
     9901 tagccaaagt tacttcaaga atagttccaa cggtttttgc agtggaagta attccgtctt
     9961 gtttgaaatt tgaaatgttt aaatagccaa tttcatcggg tttcaaggtt ttaattcggc
    10021 tttctttaat ttcagtatca ccgtgtttta ctttatttac cattctagca tcgggatctt
    10081 tgatgctttg aagtctagcg tccatttcgc ttccaggttg aaaagcattg ttgaacaaat
    10141 gataataaac acgttttaaa gtatcgggcg aattattggt gtaaaccaac ttctgtttgc
    10201 ctttgtattg atcgtttttc acattcatca ccacgtccat tttataatcg acatgttgtt
    10261 gccaatattc tgaactttgg gcaaaaccat ttgcaaaact caataaaaac acagaaatta
    10321 aaagaatttt tttcataata ataaaacaga aaatggaagg atttaactcc ctccatttta
    10381 aaattagacc taaattattg ttattttgac attttatcag ccatcaatag tgcgttgtag
    10441 gcattgacca ttttccctga tttagacaaa gttgaaaacg gacgaacatc gtttggtttt
    10501 ccgccaacaa taacatcagt agttattgct acaccagaat ccatcaaaat gtgtttcact
    10561 tgtttggctg ataattttgg ataataggaa cgaattaaag cagcaactcc agcaacattt
    10621 ggcgaagcca ttgatgttcc ttgcaataat ttataggtat tgttgggtgt tgaggcataa
    10681 atttttactc ctggtgcaaa tacatctaca ttgagttttc cgaagttaga aaatcgggct
    10741 actattttat ttccatattc ataattcaaa gcgccaatcg taatcatatt atcagcaaat
    10801 tcgtttattt tatcatcaga atcgtttgga taattattgg caacatcaat atcttttgca
    10861 tcatttccgg cagcatgaac gattaatacg tctttgcttt cggcatattt tatggcatca
    10921 taaacccatt gtttgtgtgg tgaaaaactt tttccaaaac ttccgttaat tacttttgca
    10981 ccattatcta cagcatagcg aataccaagc gcaatatctt tgtcatactc atcgccatct
    11041 ggcacagcgc gaacagtaag aatttcgaca ttgttagcaa ttccatctcc gccaattccg
    11101 ttgtttctta cttgagctac aattcctgct acgtgagttc cgtgaagaat ttcttctttg
    11161 tctggtccca ttacattgtt gttaccgtat tttgtgtcgg taatatcttc tggattatca
    11221 cccaaaactt ttcggtaatt ggttttcagg ttgtctccgt ttactacttt gatatatcca
    11281 tcaagctctt tttgaacggc tgttttaaat tctggaacag tcatttcgaa agaattgaca
    11341 cgctgaatca tcattttgct tttattgatt tcagcatctt gcgaactaat ggcatttact
    11401 tcttctttag tgtaagttga ttttccaaaa tatttggtca aaacagcatc ggcatttgtt
    11461 gcgtctgcta tcatttcttc aagacgtgct ttgcctgtag tagattttgc aatgttttta
    11521 tcatttacgg cttttgcttc ttgataagtt gcttcatcca caaggttttt gttagccaca
    11581 attctttcgt attctatgtt ttctttggta atatctccaa gaaaattcca accgtgaata
    11641 tcatcgatat agccattttt atcatcgtca attccgtttc cagcaatctc ttttttattg
    11701 gtccaaacca ctgcttttaa atcttcgtga tcgatatcaa ctcctgaatc tacaattcct
    11761 acgattactt ttttgccttt tttatctttt aataattcgg cataagctcg gtcaacactc
    11821 attccaggaa tggtatcttt aataatatct aaatggctcc aacggtttaa atcagcttct
    11881 ttaagagctg tctttctctg aacatttact ggagcttgaa tcattgtcaa gttgctatag
    11941 gaagtgtttt ttgaggctcc acaacctgct aacatcaata ctgcaaaaga ggctagtaaa
    12001 ctatttttaa aattgatcat agattgtttt ttttaagaat tttaaatttc aggtaaaagt
    12061 atgcggtttt gttatatatt aagctaatat taacattatt taaagatttt atcacaacta
    12121 taggtctctt ttaagcgaat tcctctttct gtttgttcta cagttatgat ttcgttgtgt
    12181 gcatcgtgtt ctaaaaatag ataataatta ttttcggcag cggcattcaa gaattttgat
    12241 ttttctggca tggttaacaa aggtctggtg tcatacccca taacatatgg cagaggcaaa
    12301 tgacctacgg ttggaattaa atcggccata aaacaaatgg ttttgtcttg atattttatt
    12361 tgaggtatca tttgtttttc ggtatgacca tttacataat gaattccgaa attcaattct
    12421 tcagaaaatc caaaatcatt ttcaggtagt tccaaaaaat gcaattgacc gctttcttga
    12481 ataggcaaaa tattttccga aaggaaagaa gctttttctc gaagattggg ttttgtagcc
    12541 catttccaat gattttcatt agtccaaaat ttggcatttt taaacgccac ttcatagcct
    12601 gttttgtctt tgttccaatt gacgcttcca ccacaatgat caaaatgcaa atgcgtcaaa
    12661 aaaacgtcgg taatgtcatc gcgatgaaaa ccatattttg ccaaagaatt atctaaagaa
    12721 tggtttcccc aaaggaaata gtatccaaaa aacttctcag attgtttgtt tcccattccg
    12781 ttatcaatta aaataagtcg atttccatct tcaattaata aacatcttgc ggcaagatct
    12841 atcaaattgt tggcatcggc aggattggtt ttgttccaaa tggtttttgg cacaacacca
    12901 aacattgctc caccgtcaag cttaaaattg cctgtttcta tagggtataa tttcataaaa
    12961 tgattgcttt tttttagaag aaccaaattt aagtaataca tttgttttag tattgttaaa
    13021 tagacttttt ctatccaatt atagttataa aaatggtatc ttatttgact taaagcctaa
    13081 tttgtaatac ctttgccttt tatttagaca aaatcaaaat aagctatgat aaaagtttct
    13141 gatacagcca aaaagaaaat catcgattta atgaaagagg atggttttga tgccgcagtc
    13201 gattatgtga gagtcggtgt aaaaagcggc ggatgctcgg gtttgtctta tgatttaaaa
    13261 tttgacaaaa acaaaggcga tgacgacaag atattcgtgg ataatgatat aactattgct
    13321 gtcgaaaaaa aatctttcct ttatttagct ggaacaatcc ttgaattttc aggcggatta
    13381 aacggaaaag gctttgtttt taataatcca aatgcgagta gaacttgtgg ttgcggagag
    13441 agtttctctt tgtaaaacat ttaaacaaaa attatgtcaa aatacaccga agaccattta
    13501 agagtcgaac tcgaaaccaa agaatacgag tacggatttt atactgattt agaatcggat
    13561 acctttccta ttggcttgaa cgaagatatt gttcgtgcca tttcacaaag aaaagaagaa
    13621 ccagaatgga tgaccgattg gcgcatcgag gcttttcgtg cttgggaaaa aatgattgaa
    13681 ccagaatggg ccaatgtgca ttacaacaaa cccgattttc aagcgatttc ttattactca
    13741 gcaccaaaag cagttgatcc taataaaaca ttagacgatg tagatccgga gcttttggaa
    13801 atgtataaaa agttaggcat ttcgattgac gaacagaaaa aaatgaacaa cgttgcgatg
    13861 gatatcgtgg tagattctgt ttcggtagcc acaactttca aaaaaacttt gggcgaaaaa
    13921 ggaattatct ttatgagtat ttccgaagct atcaaagaac atcccgaatt agtaaaaaaa
    13981 tatataggca ccgttgttcc tcaaaaagat aatttttatg ccgccttgaa ctcggctgta
    14041 ttctctgacg gaagtttttg ctatatccca aaaggggtgc attgcccgat ggagttatca
    14101 acttatttcc gtatcaatca agcgggaaca ggtcaattcg aaagaacctt gctaattgcc
    14161 gacgaaggta gttatgtgtc ttatctagag ggctgtacag ctcccacacg tgatgaaaat
    14221 caattgcacg cagctgttgt tgaactaata gctttagacg gagccgaaat aaaatattcg
    14281 accgttcaaa actggtatcc gggaaataaa gaaggaaaag gcggagtatt caacttcgta
    14341 actaaaagag gattgtgtga gaaagatgca aaaatttctt ggacacaagt cgaaacaggt
    14401 tctgctgtta cttggaaata tccttcttgt attttgaagg gagacaattc tgtgggcgaa
    14461 ttttattcga ttgccgttac taataatttc cagcaagccg atacaggaac caagatgatt
    14521 caccttggaa aaaacaccaa atcgaccatt atttctaaag gaatttcggc aggaaaatca
    14581 caaaatagtt atagaggatt agtgaaaatt ggtccaaatg cagacaatgc caggaacttt
    14641 tcgcaatgtg attctttgtt aatgggtaat aattgtggag cacatacttt tccttatatc
    14701 gaaagtaaaa acacttcggc caaaatagaa cacgaagcca caacgagtaa aattggcgaa
    14761 gaccaagttt tctattgcaa ccaacgtgga attccaaccg aaaaagccat tgccttgatt
    14821 gtaaacggtt ttagtaaaga tgtattgaat aaattgccaa tggaatttgc cgttgaagca
    14881 cagaaattat tggaaatttc gttagaggga agcgtaggat aatttttcta ctaattaaaa
    14941 aacgtaatga ctcgagcata gcgaacagac gaagtaatct taatacctta atggtttaaa
    15001 atataaaact aatgttaagc ataaaaaatt tacacgcctc gattggcgac aaagaaatcc
    15061 ttaaaggcat caaccttgaa gtgaaagcag gagaagttca tgcgataatg ggtccaaatg
    15121 gtgctggaaa aagtacgctt tcggctatta ttgctggaaa tgaaaactac gaagtaactg
    15181 aaggtgaaat ccttttggac aacgaagata tctctgaatt agctcccgaa gaaagagcac
    15241 ataaaggcgt attcctttcg tttcaatatc ctgttgagat accaggagtt tcggtaacta
    15301 actttatgag aacggcgatt aatgaaactc gcaaagcaaa caaattagaa gaaatgccag
    15361 ctaacgaaat gctgaaattg attcgcgaaa aatcggaatt attggaaatt gaccgtaaat
    15421 ttttgtctcg atctttaaac gaaggatttt ctggaggaga gaaaaaacgt aacgaaattt
    15481 tccaaatggc catgttggag ccaaaactcg ccatccttga cgaaaccgat tctggattgg
    15541 acattgatgc cttgagaatt gttgccaatg gtgtaaacaa attgaaaagc gacaaaaact
    15601 ctatcgttgt aatcacgcac tatcaaagac ttttagatta tatcgttcca gattttgttc
    15661 acgttctata caacggaaaa atcgtgaaat cgggcactaa agaattggcc tacgagttgg
    15721 aagaaaaagg atacgattgg atcaaacaag agcaagaagc ataagagttg tcggttgtcg
    15781 gttgtcagtt attggtaacc atcaaccacc aactatcaac caccaactaa attatggaat
    15841 taaaagaaaa attaatatcg tcttttatgg cttttgaaga gcaaatcgat gttcattcag
    15901 aactacacga tttgagaact gccgccataa aaaactttga aaataaaggc ttcccaacca
    15961 aaaaagaaga atcttggaaa tatacttcgc taaacgccat cctaaaaaat gactttaccg
    16021 tttttccaaa aagcgaaact tcgatagaat tcaaggatgt aaaaaaatat ttcatccacg
    16081 aaatagacac ctacaaaatc gtatttattg atggtgtgtt tagttctaat ttgtcatcta
    16141 caacccacca aggattggat gtttgcttga tgtcatcggc attgaacaag cccaaataca
    16201 aaatggttat tgatgcttat tttaaccaaa ttgctagcaa agatgagagt ttaacttctt
    16261 tgaataccgc tttcgccaat gaaggagctt acatcaacat tccgaaaaag acggtggtgc
    16321 aaaaacctat tgagattatg tatttctcga ctggcaacga agcggcactt ttggttcagc
    16381 ctcgaaattt agtgattgtt ggagaaaatt cacacgtaca aatcattgaa agacaccaaa
    16441 gtttgaacga aaatccggtt ttgactaatt cggttaccga gatttttgca caaaaacgcg
    16501 ctattgtaga tttctataaa attcagaatg accatctcgg agctaattta atcgacaata
    16561 cttacgtttc tcaaaaacaa gaaagtcacg tttctgttca aactttctca ttcggaggaa
    16621 atttgactag aaacaacttg aatttctatc attttggaga acgattgacc agcaccttaa
    16681 acggaatcac aatcattggc gacaaacaac acgtagatca ttatacttta gtacatcacg
    16741 ctgcgccaaa ttgcgaaagc ttccaagatt acaaaggaat tttttcagac aattctacag
    16801 gagttttcaa cgggaaggtt tatgtggaaa aagaagccca aaaaaccaac gctttccaga
    16861 aaagcaacaa cattttgttg ggagaaaaag ccaccattaa tgccaagccg caattggaaa
    16921 tttttgccga cgacgtaaaa tgttcccacg gttgcacggt gggtcaactt gacgaaaccg
    16981 caatgttcta tatgcaacaa cgtggaattc caaaaaaaga agccaaagct ttattgatgt
    17041 acgctttctc gaatgccgta atcgaaaaca tcaaaattcc agaattaaaa caaagaatta
    17101 ccaaaatcat cgccttgaaa ttaggcgtga aaattggatt tgatttgtag tttttaacca
    17161 caaagctcac taaggtttac gcaaagggcg caaagtttgt tattcaactt tgcgcccttt
    17221 gtgattttta ctttttgtgc tttgtgttta gtttatttct tcaaactccc cacaatacct
    17281 ttcaacaatc cattaaaatc aaatttgact tcttcactca atcccattct tacaataacc
    17341 aaatcttgtg aaggtagaat aaatactctt tgtccttgat agccattggc agaatacaaa
    17401 tcacgtggtg catctggata aaatccgccg gcattgagcc aaaaatgtcc tccgtaatct
    17461 ccattagaac cattagttgg tgtggctaca tattttgccc aactttcgtc aaataattgc
    17521 tcaccgttcc agttgccttt gtgcaaatac aacaatccaa attttgccca atcccttgtc
    17581 gttgcccaac tgtaggacga acccacataa ttcccagcca tatccgtttc gattatcatc
    17641 gaattcatcc caattttatc aatcaaggct gaataccaaa aattaagata ctcttgatgc
    17701 gttttgaatt gttttcgcaa aattcccgac agtaaattgg tcgttcccga agaataattc
    17761 caatgcgtgt tcggtttgaa ttttgctggt ttttctaatt gtactcgagt catatcttct
    17821 gccagaaaga gcatatttgt ggcatcgcaa attttggtat aatcttcttc ccattctaat
    17881 cctgagttca tatgaagcaa atcgttgatg gtgatatttt ttcgttcatc gtttgcccat
    17941 tcttgaattg gagccagttt attaatattt agttttcctt gtttttgtaa aattccgtac
    18001 atcgtggccg taatactttt agtcatggac caacccaaaa ttttagaatt tttatcaaaa
    18061 ccagtatcat atttctcagc aataattttg tctttataaa tcacgataac cgagcgagtt
    18121 cgcttgattt tctgcccttt tgcatcaaaa gcattggcga cagccgcatt caatttgtca
    18181 taatcgatat tgtcaaaaat ggtgtctttg ggttcgttat ttccatacgg aaaaggtaaa
    18241 ttgttgttga gttttgttct tttaggaact tcatatggtt ttgaaatatc aaaattatcg
    18301 ttgattaatg tcgctcccaa accttcacga taaatggctt ttcgttcttt taatccataa
    18361 actgaagcgg ttgcgaattt tccagtttca tcaatttggt ttttggataa agtaatcatt
    18421 ttgatatcgt tgtcgccttt ttcaatcatt tcttgcgaac gatggtcaat aaaatggcct
    18481 gaagccatac ttttagccga aaatcctgaa attaagtcca gtttaggata agtggtgtaa
    18541 cccaaataaa tcaggaatag taaaaaggca agaccgagaa acttaaagat ttttttcatt
    18601 tgaagcgaaa ttttgaagca atttaacaaa aatagcatag agaaaacata ttttgctata
    18661 aattaatact gttaaaaatt gtaaaatctt gctgttgaaa cccacctata ttcattactt
    18721 ttgtatttag aatttttcta aataaagcaa tgttagatat caataaagtt agagccgatt
    18781 tcccgatact ttccagaaca gtcaacggaa aaccattggt atatttcgac aacggagcca
    18841 cttcgcaaaa accacaaatt gtgatcgatg cgattgctaa atattatcaa gaaattaatg
    18901 ccaacattca tcgtggtgtg catactttga gccaattggc aacagatgct tatgaaattt
    18961 cgcgtagtaa aatccaaaat catatcaatg caaaatttgc ccacgaagtg atttttactt
    19021 cgggaacaac tcacggaatt aatgctatcg cgaatggttt tatttcaatt ttaaaatcag
    19081 gcgatgaggt tttggtttcg gcattggagc atcatagtaa tattgttcct tggcaaatgt
    19141 tgtgcgaaaa aacaggagcc actttgaaag tgattccaat ggatgaaaac ggcgaattga
    19201 ttatggcaga atacgataaa ttactgtcgg ataaaaccaa aatcgtgacc gtaaatcata
    19261 tttcgaatgc tttgggaact gtaaatccta ttaaatatat gattgacaaa gcccacgaat
    19321 ttggagcagc gattttgatt gatggagcgc aagctgttcc gcatttgaaa cctgatgttc
    19381 aggcattgga ttgtgatttc tatgtttttt ctggacataa aatgtgtggg ccaacaggtg
    19441 ttggaatttt atatggaaaa gaagcttggc tcaataaatt accgccttat caaggtggcg
    19501 gcgaaatgat taaggaagta actttcgaga aaaccactta tgccgatttg ccacacaaat
    19561 ttgaagcggg aacgcctgat attgctggcg gaattgtttt aggaacagca gttgattatt
    19621 tgaattcggt aggttttgac aacattcaaa aacaggaatt agaattatta gaatatgcca
    19681 caaaacaatt attagaaatt gaaggcttga aaatttatgg tactgccaag gaaaaaactt
    19741 cggtggtttc gtttaatatc gaaggcattc acccttatga tattggaaca atagtggaca
    19801 aattaggaat tgcagttcga acaggacatc attgtgccca accgattatg aattttttca
    19861 atattccggg aaccattcgt gcttcgtttt cgttttataa taccaaagaa gaaattgacg
    19921 ttatggtcga agccgtgaaa aaagctcaaa tgatgttatc ctaaaaacat aaaaaatgaa
    19981 actatatgca atgatttttc ttagtctgtt tcttggtaaa agctgtacta gccaaaccaa
    20041 aaacgacttg aaaacggctg ttttagaata taccgccaat actagaggtt tctatcaaaa
    20101 aataacgatt caaaaccaaa tggttgctgt ttcgaaagac agaagtggaa ccgaaaaagc
    20161 agtagaaacc aaaatttcgg atgccgattg gaaagaattg attagttatt tcgaaacgat
    20221 taatttggat agtttggcaa ctttgaaagc cccaaccgaa aaacggtttc acgatggagc
    20281 agcaattgct aatttagaag ttagttataa agataaaaaa tacaaaacca cctcttttga
    20341 tcacggttat ccgcctgaag cgattcaaaa attggttaca aaaataaatt catttgcaaa
    20401 aaaagaataa tgagcataaa agatatacaa gaagaaatag ttgacgagtt ctccatgttt
    20461 gatgattgga tgcagcgcta cgaatacatt attgatttgg gtaaaaattt acctctaatc
    20521 caagaagaat tcaaaactga cgataatatt atcaaaggtt gtcaatcgaa agtgtggttg
    20581 aaaggagaac aaaatgacga caaaattgtt ttcactgccg atagcgatgc tattttaacc
    20641 aaaggaatta ttgccatctt aattcgtgtt ttttcgaacc aaaaagcggc tgatattttg
    20701 aatgccgata tggattttat tgaccaaatc ggactcaaag aacatttatc tccaacccga
    20761 gccaatggat tggtttcgat gattaaaaat ataaaaatgt acgcattggc attcgatgca
    20821 aaaaaataaa aattatggaa caaacaataa acaccgacca attagggata gatatcgtag
    20881 ccaaactaaa aaccatctac gacccagaaa ttcctgttga tatttatgaa ttaggattga
    20941 tttatgatgt gatgatcagt acggattatg aagtaaaaat cctaatgact ttaacttctc
    21001 caaactgccc tgtggcagaa agtttaccaa gagaagtaga agaaaaaata aaatcaatcg
    21061 aacacatcaa aggagccgaa gtagaaatta cttttgaccc gccttggaat aaagatttga
    21121 tgagcgaaga agccaaatta gaat
//
LOCUS       DAOSNN010000015        21038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_2523_length_21038_cov_5.933184, whole genome shotgun sequence.
ACCESSION   DAOSNN010000015 DAOSNN010000000
VERSION     DAOSNN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 21038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21038
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2523_length_21038_cov_5.933184"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1459
                     /locus_tag="PLQ70_01630"
     CDS             134..1459
                     /locus_tag="PLQ70_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQE33345.1"
                     /translation="MKKTIMALSASLLLGGIASAQKVDFEEYTLNNGLHVILHNDSSA
                     PVVVTSVMYHVGAKDENPERTGFAHFFEHLLFEGTQNIKRGEWMKIVTANGGVNNANT
                     TDDRTYYYEVFPSNNLELGLWMESERMLHPIINQIGVDTQNEVVKEEKRTNFDNRPYG
                     NILMAVKENLFKNHPYRWNTIGSMKDLDAATLEEFQAFNKKFYTPNNAVLVVAGQIDV
                     AQTKEWIQKYFGSIPKGEILKKQVFAEEPITKTIKATYQDPNIQIPMLVTAYRTPSMK
                     TRDAKVLDLISSYLSDGKSSKLYKKIVDQKKIALQIGAVGLSEEDYGMYIIYGLPMTP
                     ATSADLLKEIDEEVVKIQTNLISEKDFQKLQNTFENQFVNANANVEGIAENLATYHLL
                     YGDVKLINNQIDLYHSITREEIREVAKKYLNPNQRLILDYVPSTEQAQN"
     gene            1470..>1908
                     /locus_tag="PLQ70_01635"
     CDS             1470..>1908
                     /locus_tag="PLQ70_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQE33346.1"
                     /translation="MKKTSVLILIMFITGIMQAQDRPQPKPGKAPVVNIKKPQTFILS
                     NGLKVMVVENHKLPRVTFNLTLDNPPFAEGNKKGVDDLTSSLIGNGSKKIAKDIFNEE
                     IDFFGANVNFSSQGAYANTLSKYATQILELMADGALNPNFTQVE"
     assembly_gap    1909..1918
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1919..3520
                     /locus_tag="PLQ70_01640"
     CDS             <1919..3520
                     /locus_tag="PLQ70_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQE33347.1"
                     /translation="EKAKMIEGLKAEEKSVPTINNRVTDALAFGKNHPSGEFVTVETL
                     NNVTLADVQTNYQNYFVPENAYLVIIGDVKYNKIKPLVEKLFGSWTKKSTPKLAYTDP
                     KNVEFTQINFIDMPNSVQSEIALVNTVNLKMSDPDFFPAALATYILGGDFNSYLNMNL
                     REKHGWTYGANAIIGSGKYVTKLKSASAVRNVVTDSAVVEFIKEIKKIRIEKVSDELL
                     NNVKAGYVGRFVMQVQKPQAIARYALNIETEKLPADFYENYIRNINAVTPEQIMQAAN
                     KYFLIDNTRIVITGKGSDVLPGLEKLKIPIFYFDKYGNPIEKPVAKKEVPAGITAKTV
                     LDNYIKAIGGEKAVSTVKTIAMLGSTTIPQAPSPLTFTNKSDSRGKIMVEIAMGPMSL
                     MKQVVTEKGAYVVQQGQRKNIEGADLAEMQASAKPFEELQLAKKAEIAITGIETINGN
                     DAYAIQNGKTTLYYDTKSGLKVAETKTVEQAGNKISQTTNYGDYREVKGVKMPFNIIQ
                     NVGFELDIKMSEVKINEGVSDADFQ"
     gene            complement(3657..4523)
                     /locus_tag="PLQ70_01645"
     CDS             complement(3657..4523)
                     /locus_tag="PLQ70_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQE33348.1"
                     /translation="MKSKQLKWVYLIALAFVWGSSFILIKRGLVGLTPMQLGSLRIIF
                     AALFLLLIGFKSLSKIPLHQWKYIALTATLGTFIPAFLFSIAQTQIDSSVSAILNSLT
                     PLNTMILGALAFGLSFKRSQVFGVIIGLIGTFLLIINGAIHHPEQNYYYAILVIIASV
                     CYATNVNLIKKYLSDLSPLSITTGNFLVLLFPALIVLFFSGFFEVISVESVQQSVLYI
                     MILGVVGTGIANIIFFKTIQISSPVFATSVTYLIPVVAFSWGLLDNEMLTPVQFLGAF
                     IILVGVYLSAKK"
     gene            4625..4996
                     /locus_tag="PLQ70_01650"
     CDS             4625..4996
                     /locus_tag="PLQ70_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HQE33349.1"
                     /translation="MNFIKSISAILFASLLFVGCKDKTNETSSDTTTATPKVKKEIAA
                     ANLQTASFTIKGMTCAIGCAKTIQEELNGLDGVQKATVDFEKEFATVSFDKTILSPEK
                     ITKVVEAAADGKSYKVSNMKI"
     gene            complement(5047..5802)
                     /locus_tag="PLQ70_01655"
     CDS             complement(5047..5802)
                     /locus_tag="PLQ70_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33350.1"
                     /translation="MNKKPLMLTLILAFLGCVLIGLSVYFFFDGSFDANEKIETAEKE
                     LEVANKKVENLSQELRLMTKSNGEEKNNSIGAFSKLASNNIPIVQITSVDLGEDVGVE
                     GNEKKTNRLTYSHEIRFFILNIGKSALKEVIFSIKDIYNEPKWRKKNKKARVEYDCFG
                     QVINSAEFGSYNNIEINTLNLKSKKIAYSSNLPSSFGDGEYFFDIILEWEKGSYQIKV
                     VIVEAAGKLNFKYEFYDADGELLDLKKLPNNSK"
     gene            complement(6011..6092)
                     /locus_tag="PLQ70_01660"
     tRNA            complement(6011..6092)
                     /locus_tag="PLQ70_01660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6056..6058),aa:Leu,seq:tag)
     assembly_gap    6094..6193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6356..7744
                     /locus_tag="PLQ70_01665"
     CDS             6356..7744
                     /locus_tag="PLQ70_01665"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HQE33351.1"
                     /translation="METLKSYVQEHKNRFINELIELLKIPSVSADTAYHQHIITTANA
                     VKASLEKAGCDFVEICETPGNPIVYGEKIINKDLPTVLVYGHYDVQPADPIELWTSPP
                     FEPVIKKTEIHPDGAIFARGSCDDKGQMYMHVKALEYMIQSNTLPCNVKFMIEGEEEI
                     GSNSLAWFVERNQEKLKNDVILISDTGMISNQQPSITTGLRGLSYVEVEVTGPNRDLH
                     SGLYGGAVANPINVLAKMIASLHDENNHVTIPGFYDKVEELSLEERAEMAKAPFNLEN
                     YKKSLDLNDIYGEKGYVTNERNSIRPTLDVNGIWGGYTGEGAKTVIASQAFAKISMRL
                     VPNQDWEEITELFTQHFINIAPIGVKVKVKPHHGGQGYVTPTDSIGYQAANKAYTETF
                     GVPAIPVRSGGSIPIVALFEKELKSKTILMGFGLDSDAIHSPNEHFGIFNYLKGIETI
                     PLFYKYFVEMSK"
     gene            complement(7897..9252)
                     /locus_tag="PLQ70_01670"
     CDS             complement(7897..9252)
                     /locus_tag="PLQ70_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQE33352.1"
                     /translation="MRAILIIGAGRSSSYLIQYLLNKSVEEDLHLIIGDLSLESAQKK
                     TNNHPNAMAISLDIFDERSRKEAIQKADIVISMLPAHLHIEVARDCIVCKKNMVTASY
                     ISDAMQELDALVKENNLVFMNEVGLDPGIDHMSAMKVIHEIKNKGGKMLLFESFCGGL
                     VAPESDTNLWNYKFTWAPRNVVLAGQGGAAKFLQEGTYKYIPYCNLFRRTEFLEVEGY
                     GKFEAYSNRDSLKYLDVYGLNDVLTLYRGTIRRVGFSKAWNVFVQLGMTDDSYVMEDS
                     ENMSYRQFVNSFLPYHPTDSVEIKMRLILKIDQDDIMWDKLLELDLFNPDKKVGLKNA
                     SPAQILEKILKDRWTLEPNDKDMIVMYHKFGYEIDGIKQQIDSKMVCMGEDQTYTAMA
                     KTVGLPVAMATLLILNGKIKTTGVQLPIREEVYLPILKELEEYGVVFKEQIVPYLGYN
                     A"
     gene            9406..9792
                     /locus_tag="PLQ70_01675"
     CDS             9406..9792
                     /locus_tag="PLQ70_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HQE33353.1"
                     /translation="MDKKIISTGAIFGMLAIIFGAFGAHALKKALAVEELTTFETGVK
                     YQMYHALFLLFIGLTSQLSEKAKKTIYYLVVTGVIFFSGSIYGLATNKLSSFDFKTIG
                     FITPIGGLLLILAWGILLVNLLKKKS"
     gene            9969..11069
                     /locus_tag="PLQ70_01680"
     CDS             9969..11069
                     /locus_tag="PLQ70_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HQE33354.1"
                     /translation="MLKSFFILCSGADKTLLEGCSEGEQTKYVGIGATVFFTAVMAFI
                     ASSYALFTVFDNPFIAMGFGIVWALLIFNLDRFIVSTIRKRDRFKSEFLQASPRIILA
                     IIIAIVISKPLEIKIFEKEINTVLLKEKNAMALKNKKEVANYFKSDLDKNKAETDKLK
                     AEIALKEKEVNTLYETYIAEAEGTKGTMKLGKGPVFKEKIAKHNLASAELDSVRKTNL
                     AKIAVNDKKAITLQADLDKKVTETQPIIDGFDGLMARINALNKLPLIPSLFIMLLFLA
                     IETSPIIAKLLSQKGEYDYKLEDLETALKVTLLQDKYQRDLLVKTSAAMHDKVYEDIA
                     QDKKLYDLQRQKATELLELQAHNFVEKQKRTL"
     gene            complement(11130..11819)
                     /locus_tag="PLQ70_01685"
     CDS             complement(11130..11819)
                     /locus_tag="PLQ70_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQE33355.1"
                     /translation="MIDNIKKFLNEEQDPKAIEKITSKLNDLLMKNEEIGYIAVQKKP
                     AITVFPDSIVVTNKRIIICQPKNMGLSMNFTDFTWDEIEGTFMKENILGSEFSFSTKT
                     QIQVSIDYIPKIQARKISTYAKEQLDLLKNPVVAEEAKPQIPEYIPEEIVEEVETEEV
                     VNYAEIMPAVANYAEPIVENTAASTEKPTTELTQDELFAKLQNYKKLLDNGLILQGEY
                     DAFKKEILSLM"
     gene            complement(11869..12240)
                     /locus_tag="PLQ70_01690"
     CDS             complement(11869..12240)
                     /locus_tag="PLQ70_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HQE33356.1"
                     /translation="MNLETYYEYCLSKKGTTEHFPFDQDTLVFKVGGKMFALSSLNQW
                     EKGEPAVNLKCNPDYAQELRAQYSDVKPAFHMSKVHWNTLVINNEIPDKLVKEWIDDS
                     YDLVFKSLTKKIQTEILQLEN"
     gene            complement(12237..12638)
                     /locus_tag="PLQ70_01695"
     CDS             complement(12237..12638)
                     /locus_tag="PLQ70_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE33357.1"
                     /translation="MITVSTDKTKLNVPFIQDFLKDIYWAAGRTIDEVQTTIDSSVCF
                     GIYLDDIQIGFARVVTDYVVFAYLMDVFIAEEHRGNGYSSILIEAMMNEPVLKNIKIW
                     RLATSDAHFLYEKFGFKALAHPEKMMEKVTI"
     gene            complement(12709..13842)
                     /gene="hemW"
                     /locus_tag="PLQ70_01700"
     CDS             complement(12709..13842)
                     /gene="hemW"
                     /locus_tag="PLQ70_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQE33358.1"
                     /translation="MSGIYIHIPFCKQACHYCDFHFSTSMKKKDEMVLALVKEIQMRK
                     SEFKDEVVETIYFGGGTPSRLQIVDLKLQLDSIYKNYKVSENPEITLEANPDDLSEGY
                     LIELSKIGINRLSIGIQSFYEDDLQMMNRAHNSVEAKKSLELATKYFDNISLDLIYGI
                     PGMNNEKWKQNIETALSFGIPHISSYALTVEPKTALNKLIQTGKIAQPKDEVAEEHFQ
                     ILVETLENNGFIHYELSNFGKENYFSKNNSAYWLGKKYIGIGPSAHSYDGISRSWNVS
                     NNSIYLKSLEENKLPSEIEILSKSDRYNEYIMTSLRTIWGVSLDRILDEFGNEYLDYL
                     QKQAHKFINDDLLSIENKILKPTQKGKFLTDGIASDLFYLNLE"
     gene            complement(13852..14295)
                     /locus_tag="PLQ70_01705"
     CDS             complement(13852..14295)
                     /locus_tag="PLQ70_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33359.1"
                     /translation="MKKELRSSVQCSGFYKSIIMFKELSFIGFIFIPLIFTISRKVLR
                     ERKSFLIILSFSFLASILALCEMNSETSDSPNFILFWFCPLYSLLLYRLMLIPFWARK
                     KRDPKIPPRELFYSGNVNLFADKFFGFVFMLLSTLLPMILIINLS"
     gene            complement(14273..14827)
                     /gene="ruvC"
                     /locus_tag="PLQ70_01710"
     CDS             complement(14273..14827)
                     /gene="ruvC"
                     /locus_tag="PLQ70_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQE33360.1"
                     /translation="MTNERIILGIDPGTTIMGFGLIRIVNKKMEFLQLNELQLSKYDN
                     HYQKLKIIFERTIELIDTHHPDEIAIEAPFFGKNVQSMLKLGRAQGVAMAAGLSRDIP
                     ITEYEPKKIKMAITGNGNASKEQVAKMLQQLLGLKQLPKNLDSTDGLAAAVCHFFNSG
                     KVIGEKSYSGWDAFVKNNEERIKK"
     gene            complement(14973..16766)
                     /locus_tag="PLQ70_01715"
     CDS             complement(14973..16766)
                     /locus_tag="PLQ70_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQE33361.1"
                     /translation="MKNLSSSFVLILLFLAISLPVFSQNKEIPLWDKIPGVIEASDYK
                     QEPRLDAQGNITGIRKVTEPTLKIFLAANKNAKNAAVIICPGGGYALLSHEKEGDKVA
                     AWFQSIGISAFVLKYRLPSDIIMKDKTIGPLQDAQEAIRTLRRRADEWNLDPTKIGVI
                     GFSAGGHLAATVSTRYNDKVYDSKDNVSARPDFSILIYPVISMENGITHKGSQENLLG
                     KNASSELIAKYSNEKQVDANTPKTFLVHATDDKAVPVENSLNYYLALKNQKVPVEMHI
                     YENGGHGFGLGVQGTNKNWPAACEKWLISNGFIPKSDAYVFTYFKGNGEDGLHLAYSE
                     DGYKWEALKKDASFLTPEVGKDKLMRDPCVIKGGDGLYHMVWTVSWTDKGIGYASSKD
                     LIHWSKQEFLPVMEYEKKTRNTWAPEITYDEKSKNYMIYWASTIEGKFTETQSTEEKG
                     YNHRIYYTTTKDFKSFKKTKLLYEPGFNVIDASIVKDEKGYTMFLKDETKVPVQKNLK
                     IATSKNLEGPYTKASEPITGKYWAEGPTAIQINGQWLVYFDKYTQKKYGAVKKTAEGW
                     EDISEQVSFPQGTRHGTVLKVPNSVIAELKK"
     gene            complement(16818..17942)
                     /locus_tag="PLQ70_01720"
     CDS             complement(16818..17942)
                     /locus_tag="PLQ70_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HQE33362.1"
                     /translation="MKKLFLFFAICFLNFNNANAQQLFDKKQVLKQMMLANDYFMKKW
                     PEVSKTIVTNKERPSNIWTRGVYYEGLMALHEIYPKEEYYRYAYDWSEFHKWSFRNGS
                     ANRNADDYCAAQTYIDLYNLEPDSKKLKSTRATLNMLLNTPQNDDWSWIDAIQMGMPV
                     FAKMGVLEKDNRYFEKMYQMYMYSRNNHGDHGLYNPKDGLWWRDADFDPPYKEPNGED
                     CYWSRGNGWVIAALAKVLTIIPENAPHREQYVKDLKTMAEALVKVQRPDGFWNVSLHD
                     PTNFGEKETSGTALFVYGIAYGVNSGILKKETYLPVIQKAWNAMTKESLHENGFLGFL
                     QSTGKEPKDGQPLSYDKIPDFEDYGLGCFLLAGSEIYKMN"
     gene            complement(17967..20699)
                     /locus_tag="PLQ70_01725"
     CDS             complement(17967..20699)
                     /locus_tag="PLQ70_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQE33363.1"
                     /translation="MRLKKNYSLFIVAFLFALSAFAQDTAPSPWPKSSNVNQPWARWW
                     WMGSAVDKSNLKRSLEDFHNAGIGGVEITPIYGVKGEENNFIDYLSPKWMEMLDYSVQ
                     VADSLHMQVDMVLGTGWPYGGSHVTLPYAATKLIVEKYQLTKNETFDKAIEIDKAKEK
                     IPADLLYVMAYSSDGKCVNLTQELKGNVLQWKAKKTDYTIYAVFSGKTGQQVKRAAPG
                     GKGYTLDHYSEEALNAYVVPFNAAFKGREGKVRAIFNDSYEVYGTDFTPRFFEEFKTR
                     RGYDLKEKLPLLLSETDNAIGNRIKSDYRQTISDLLLNKFDKPWTTWANSKNFKTKLQ
                     AHGSPGNLIDLYASADIPECETFGSMPFDIPGFRREKEDIREGDADPVMLKFSSSAAH
                     ISGKPLVSSETFTWLRDHFKIALSQCKPEAEELLLNGINHIFLHGSTYSPDRAKWPGW
                     KFYASANFNNTDPTWEDAPALFSYISNCQAMLQQGQSDNETLVYWPIFDTWDKYQKGT
                     LFFEFKIHSLSQWLHDTSFYNTTKNLMGKGYGVDFISDNFIAQAQVKEGKIVLPGGTF
                     KSIVIPACEKMPLSTLQKLIDLQKAGGKIIFEGLPKSVPGYFEFENQNQKLAALLDEN
                     KTAIQPVSDIFKALEEAQTYPETLVSSGLKYTRRTVDGEKLYYLVNHSAKIIEGFIPL
                     EVGNKDVVIFDPLTKEYGNAVVTKTDKNTLVKLKIEPGQSLFLKTEKTASQKKWNYFE
                     KTANAITLNGKWQLTFDKGGPKLPATATVSNLESWTKLSPEAEAFSGTATYTLQFDNT
                     NNTVENWSLNLGDVRESAKVWLNDQFIGTAWSVPYQLNIGKLKKGKNTLKVQVTNLGA
                     NRVRDMELKGEEWKIFYEINMVDKDYNKFDATKWKPTPSGLLGPVTITPLKSQN"
BASE COUNT         6913 a         3885 c         3425 g         6705 t
ORIGIN      
        1 aagtaaattt tcatcaaatt gttaaacttt acataaagta agaaaaattt gtcattgaat
       61 attaaaaagt gttacatttg gtgtaacaat ataataagtt atcacaccta ttgaaaaaaa
      121 attattaatc cttatgaaaa aaacaataat ggctttaagt gcttcacttt tgctaggagg
      181 aattgcctca gctcaaaaag tagatttcga agaatacaca ttaaataacg gattacacgt
      241 tattttgcac aacgattctt ctgctccagt ggttgttact tcggtaatgt atcatgtagg
      301 tgccaaagat gaaaatcctg aaagaacagg tttcgctcac ttctttgagc atttgttgtt
      361 cgaaggaacc caaaacatca aacgtggaga atggatgaaa attgttaccg ctaatggcgg
      421 cgttaacaat gccaatacaa ccgatgacag aacctactac tacgaagtat tcccatctaa
      481 taatttagaa ttaggacttt ggatggaatc cgaaagaatg ttgcatccaa ttatcaatca
      541 aataggggtt gacacgcaaa atgaagttgt aaaagaagag aaaagaacca atttcgacaa
      601 tcgcccttac ggaaatatac taatggctgt aaaagaaaat cttttcaaaa accatcctta
      661 tcgctggaac actattggct caatgaaaga tttagatgcc gcgacattag aagagtttca
      721 agctttcaac aaaaaattct atactccaaa caatgcggtt ttagtagttg caggtcaaat
      781 agacgttgcc caaaccaaag aatggataca aaaatatttc ggttcaattc caaagggaga
      841 aatcttaaaa aagcaagtct ttgccgaaga accaataact aaaaccataa aagcaacata
      901 tcaagaccca aacattcaaa tcccgatgtt ggtgacagcc tacagaactc catcaatgaa
      961 aactagggat gctaaagttc tggatttaat ttcttcttat ttaagtgacg gaaaaagttc
     1021 taaattatac aagaaaatag tagatcagaa aaaaatagcc ttgcaaattg gcgctgttgg
     1081 tttgagcgag gaagactacg gaatgtatat tatttatggc ttgccaatga ctcccgcaac
     1141 ttcggccgat ttattaaaag aaatcgatga agaagtagtg aaaattcaaa ctaatttaat
     1201 ttctgaaaaa gactttcaaa aattacaaaa cactttcgaa aatcaatttg taaatgccaa
     1261 tgccaatgtg gagggaattg ccgaaaattt ggctacctac cacttattat atggagatgt
     1321 aaaactaata aataaccaaa ttgacttgta tcactccata acaagagaag aaattagaga
     1381 ggtagccaaa aaatatttaa acccaaatca aagattgatt ttagactatg ttccttcaac
     1441 tgagcaagct caaaactaag acatttatta tgaaaaaaac aagcgtccta atactcatta
     1501 tgttcataac cggaattatg caagcacaag accgtccgca accaaaacct ggaaaagctc
     1561 cagtagtaaa catcaaaaaa ccacaaactt ttatcttatc gaatggatta aaagtaatgg
     1621 tagtagaaaa ccataaattg ccccgagtaa catttaacct tactttagac aaccctcctt
     1681 ttgccgaagg aaacaaaaaa ggtgtagatg atctaacaag tagcttgata ggaaatgggt
     1741 caaaaaaaat cgcaaaagat atttttaacg aagaaataga tttctttgga gccaacgtca
     1801 actttagttc gcaaggtgct tatgccaata cgctctctaa atatgccacc caaatactcg
     1861 aattaatggc tgatggcgca ttgaatccaa actttaccca agtagaatnn nnnnnnnnga
     1921 aaaagccaaa atgatcgaag gcttaaaggc cgaagaaaaa agcgttccaa ccatcaacaa
     1981 cagagtaacc gatgctttgg ccttcgggaa aaatcatcct tcaggtgaat ttgtaacagt
     2041 agaaacctta aacaatgtta ctctagccga tgttcaaacc aattatcaga actattttgt
     2101 accagaaaac gcttatttag ttattattgg cgatgtcaaa tacaacaaaa taaaacctct
     2161 tgtcgaaaaa ttatttggtt cttggacaaa aaaaagcact cctaaattag cttataccga
     2221 cccaaaaaac gtggagttca cccaaattaa ttttatagac atgcctaatt ctgttcaatc
     2281 agaaattgca ttagtcaata ctgttaattt aaaaatgagc gatcctgact ttttccctgc
     2341 tgcccttgct acctatatat taggaggcga tttcaacagt tatttgaata tgaatctaag
     2401 agaaaaacac ggctggactt acggagccaa cgctattatt ggatctggaa aatatgtaac
     2461 taaattaaaa tcggcttcgg cagtaagaaa tgtggttact gatagcgctg tggtggaatt
     2521 tatcaaagaa attaaaaaaa ttagaatcga aaaagtaagc gatgaattgc ttaataatgt
     2581 caaagcaggt tacgtaggac gatttgtaat gcaggtccaa aaaccacaag ccatagctag
     2641 atatgcttta aatatcgaaa ccgaaaaact tccagcagac ttttacgaaa attacattcg
     2701 caatattaat gcggtaactc cagaacaaat aatgcaagct gccaacaaat atttccttat
     2761 cgacaatact cgaattgtga tcacaggaaa aggttcggac gtattgcccg gtttagaaaa
     2821 actaaaaatt ccaatatttt attttgataa atacggaaat ccgattgaaa aacctgttgc
     2881 caaaaaagaa gttccagcag gaataaccgc aaaaacagtt ttagataatt acatcaaagc
     2941 aattggtggc gaaaaagccg ttagcactgt aaaaacaatt gcaatgctag gctctacaac
     3001 tattccgcaa gcaccttctc ctttgacttt tacaaacaaa tccgattcaa gaggcaaaat
     3061 aatggtcgaa atagctatgg gaccaatgag tttaatgaaa caagtcgtta ctgaaaaagg
     3121 agcttatgta gtgcaacaag ggcaaagaaa aaacattgaa ggagcagatt tagccgaaat
     3181 gcaagccagt gcaaaacctt ttgaagaact acaattggca aaaaaagcag aaatagcaat
     3241 tacaggaata gaaaccatta atggcaatga tgcttatgcc atacaaaacg gtaaaacaac
     3301 tttgtattat gacaccaaat caggacttaa agttgctgaa actaaaaccg tagaacaagc
     3361 tggaaacaaa atatctcaaa ccactaatta tggagattat agagaagtga aaggagtgaa
     3421 aatgcctttt aacatcatcc aaaatgtagg ttttgaatta gacatcaaaa tgtctgaagt
     3481 aaaaattaac gaaggagttt cagatgcaga tttccaataa ttagctgtta gttataataa
     3541 ttacaaaaac ggaaacctcc tgagaaatca agaggttttc gtttttatac ctagaacaat
     3601 taatattcat caaaccagac actgaacacc actcactgaa cactgaatac tgaccactac
     3661 tttttagccg acaaatagac tccaaccaaa ataataaaag cacccaaaaa ctgaactgga
     3721 gtaagcattt cattatctaa caatccccaa gaaaaagcaa ctacaggaat caaataagtg
     3781 accgaagtag caaaaactgg cgacgatatt tgaatagttt taaagaaaat gatattggca
     3841 attccagttc ctacaactcc caaaatcata atatacaaaa ccgattgttg aacactttca
     3901 accgaaatta cctcgaaaaa accagagaaa aacaaaacaa tcaaagctgg aaaaagcaaa
     3961 accaaaaaat ttcctgtcgt aatgctcaac gggctcaaat cggataaata tttcttgatt
     4021 aaattgacat tcgttgcata acaaactgaa gctataataa ctaaaatggc gtaataataa
     4081 ttttgttcag gatgatgaat agcaccgttt ataattaaca aaaatgttcc tattaagcca
     4141 ataataactc caaaaacttg agacctttta aagcttaatc caaaagctaa agctcccaaa
     4201 atcatagtgt ttaatggcgt aagagaattt agaattgcac tcacagaact gtcgatttgg
     4261 gtttgtgcaa tcgaaaaaag aaatgccgga ataaaagtcc ctaaagtggc tgtcaaagcg
     4321 atatatttcc attgatgaag cggaattttt gacaaacttt tgaaaccaat tagcagtaaa
     4381 aatagggctg caaaaatgat acgtaacgac cctaattgca tcggcgtcaa accgaccaaa
     4441 cctctcttga ttaagataaa tgaactaccc caaacaaatg ctaaagctat caggtaaacc
     4501 catttcaatt gtttcgactt catacactta aattttggct caaaattgta attattttat
     4561 gaattaccgg cacttttttt attaaatttg tttattaatt tttaatcatt aatccccaac
     4621 aataatgaat ttcatcaaat ctatttcagc aatactgttt gcaagcctat tgtttgtggg
     4681 ttgtaaagat aaaacaaacg aaacctcttc agatactacc actgcaactc caaaagttaa
     4741 aaaggaaata gcagcagcta atttgcaaac agcaagtttc actatcaaag gaatgacttg
     4801 tgccattggc tgtgccaaaa caatccaaga agaattaaac ggacttgatg gcgtacaaaa
     4861 agcaaccgta gattttgaaa aagaatttgc aacggtttct tttgacaaaa ccatattatc
     4921 cccagaaaag ataacaaaag tggtcgaagc agctgcagac ggaaaatcgt ataaagtttc
     4981 taatatgaaa atttaatttt tttattggta ctaaaccaaa aagcttctcc aattgagaag
     5041 ctttttttat ttgctgttgt ttggcagttt tttcaaatca agaagttcac cgtctgcatc
     5101 ataaaactcg tatttgaagt ttaatttgcc tgctgcttct acaataacaa cctttatttg
     5161 atacgagcct ttttcccact ctagaattat atcaaaaaaa tattcgccat caccaaaact
     5221 actaggaaga ttgctagagt atgctatttt tttggattta aggttcaatg tattaatctc
     5281 aatattgttg taagaaccaa actcggcgct attgataact tgcccaaagc aatcatattc
     5341 aactctagct tttttatttt ttttcctcca ttttggttcg ttataaatat ccttaattga
     5401 aaaaataact tcctttaatg cacttttacc gatatttaaa ataaaaaatc taatctcgtg
     5461 actgtaagtt aatcgatttg ttttcttttc attgccctca actccaacat cttcaccaag
     5521 atctacagat gtaatctgta caataggaat attattactt gctaatttac taaaagctcc
     5581 aattgaatta tttttttctt ctccattgct tttagtcata agtcgcaatt cttgacttaa
     5641 attttcaact tttttatttg ctacttccaa ttctttttcg gctgtttcta ttttttcatt
     5701 ggcatcaaaa cttccatcaa agaagaaata gacagaaagt cctatcaaaa cacatcctag
     5761 aaatgccagt attaatgtta gcataagtgg ttttttattc attacaaaaa tcggtttgag
     5821 aattattata ttctacatta atctgagttt acaaatatat taaaatttgc tgtttaactg
     5881 attataaacc aatataatgt aagattttat aaataaaaaa taagtttaaa aaattagaaa
     5941 ctacattcta ttaagaaaat gatttaaaca taagcataaa aaaagcttct cgattgagaa
     6001 gcttttttcg tgcggataat aggactcgaa cctacacgcc ttgcggcacc agatcctaag
     6061 tctggcatgt ctaccaattt caccatatcc gctnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnccttgcg gcaccagatc ctaagtctgg catgtctacc aatttcacca
     6241 tatccgctaa aattgtgagt gcaaagataa tcataagttc caattttcaa aggaatttat
     6301 tcaaaaatat ttacgctctt ttttgtactt ttgaaacact taaatttttc aaataatgga
     6361 aaccttaaaa tcctacgttc aagagcacaa aaaccgtttc atcaatgaac taattgagct
     6421 tctaaaaatc ccttccgtta gtgccgatac cgcttatcat caacacatta tcacaactgc
     6481 caatgccgta aaagccagtt tagaaaaagc aggatgcgat tttgtagaaa tttgtgaaac
     6541 accaggaaac ccaattgtct atggcgaaaa aataataaat aaggacttac caaccgttct
     6601 agtttatgga cattatgacg ttcaacccgc cgatcccatc gaattatgga cttctccacc
     6661 attcgaaccc gtgatcaaaa aaaccgaaat tcatcccgat ggagccattt ttgcaagagg
     6721 ttcttgtgat gacaagggcc aaatgtatat gcacgtaaaa gcattggaat atatgattca
     6781 gtccaatact ttgccttgca acgtcaaatt tatgattgaa ggcgaagaag aaattggatc
     6841 taatagtttg gcttggtttg tagaacgcaa ccaagaaaaa ctaaaaaatg atgtgatttt
     6901 aatttccgac accggaatga tttccaacca acaaccttct atcaccactg gattgcgtgg
     6961 gttgagttat gtggaagtgg aagttacagg tccaaacaga gatttgcatt ctggacttta
     7021 tggtggcgca gttgctaatc ctattaatgt tttggcaaag atgattgctt cacttcacga
     7081 cgaaaacaat cacgtgacca ttcccggttt ttatgacaaa gtcgaagaat tgtcactgga
     7141 agaaagagcc gaaatggcca aagcaccttt caatttagaa aattataaaa agtcattaga
     7201 cttgaatgat atttatggcg aaaaaggata tgtaaccaac gaaagaaatt caatccgacc
     7261 aacacttgac gtgaacggaa tttggggcgg ttatactggc gaaggagcaa aaacggttat
     7321 tgcaagccaa gctttcgcca aaatttcaat gcgattggtt cccaatcaag attgggagga
     7381 aatcaccgaa ttgtttaccc aacattttat caacattgct ccaatcggag tgaaagtgaa
     7441 agtaaaacca catcacggtg gacaaggtta tgtaacacca acggacagca ttggctacca
     7501 agccgccaac aaagcctaca ccgaaacctt tggagttcca gcaattccag tacgttctgg
     7561 aggaagtatt cctattgttg cgttgttcga aaaagaacta aaaagcaaaa ccatcctaat
     7621 gggatttgga ttagacagcg atgccattca ctcacccaac gaacatttcg gaatttttaa
     7681 ttacctgaaa ggaattgaaa cgattccgtt gttttacaag tattttgtgg agatgtcgaa
     7741 gtagttgttt ttggacacag atgaaacaga ttcgccaaag cgaaaacgcg gataaaaacg
     7801 gattttattt ctagttaaat gcaattttag aaatcatttt ttatccgtgt ttttaggtgt
     7861 taaatctgtt ttatccgcgt ccaattttaa tatccatcaa gcattatacc ccaaataagg
     7921 cacaatttgc tccttaaaaa caactccata ttcttccaat tctttcaaaa tgggcagata
     7981 cacttcttct cgaataggaa gttgaactcc cgtagtttta atttttccgt tcaagattaa
     8041 taatgtagcc atagccactg gcaaaccaac ggtttttgcc attgctgtat aagtttgatc
     8101 ctcgcccata caaaccattt tggaatctat ttgctgtttt ataccatcta tttcataacc
     8161 aaatttatga tacatcacaa tcatatcttt gtcattgggt tccaatgtcc atctgtcttt
     8221 caatattttt tcgagaattt gagctggact ggcgtttttc aaacctactt ttttatccgg
     8281 attgaacaaa tccaattcca ataatttatc ccacattata tcgtcttgat cgattttaag
     8341 tatcaaacgc atcttgattt caacagaatc ggttggatga tacggcaaaa aagaattcac
     8401 gaattgacgg taactcatat tttcggaatc ttccattaca taactgtcat cagtcattcc
     8461 gagttgaacg aacacattcc aagctttaga aaaaccgact ctacgaatgg ttcctcggta
     8521 caaagtcagc acatcattca atccataaac atcaagatat ttcaaagaat cgcggttcga
     8581 ataggcttca aattttccat aaccttcaac ctctagaaat tcggttcggc ggaataaatt
     8641 acaatacgga atgtatttat aagttccttc ttgcagaaat tttgccgctc caccttgtcc
     8701 cgctaagacg acatttctag gagcccaagt gaatttataa ttccacaaat tcgtatccga
     8761 ttcaggagct acaagacctc cgcaaaaaga ttcaaacaaa agcattttac ctcctttatt
     8821 cttgatttcg tgaatgactt tcatcgcact catatgatca attccgggat cgagacccac
     8881 ttcgttcata aaaaccaaat tattttcttt gaccaaagca tccaattcct gcattgcatc
     8941 gctaatatag gaagcagtaa ccatattttt tttgcaaaca atacaatctc tagccacttc
     9001 aatatgcaaa tgggctggca acatcgaaat aacaatatcc gctttttgaa ttgcttcttt
     9061 tctggaacgt tcatcaaaaa tatctaatga aattgccata gcatttggat ggttgtttgt
     9121 ttttttttga gcggattcta atgacaaatc cccaataata agatgcaaat cttcttcaac
     9181 cgacttgttt aaaagatatt gaattaaata agaagatgat cttccagctc ctatgattaa
     9241 aattgctctc attgttgtaa aatatttaac acaaatatat gtaattgttg catttacaac
     9301 aaattcaaat gatttaaaat caaaaataat acaagtaaaa atcattattc taattgattt
     9361 gaatttgaca agaaagagat acttttgtac tgtaaataaa ttaaaatgga taaaaaaata
     9421 atttctacag gagcaatttt tggaatgttg gctatcattt ttggcgcatt tggcgcacac
     9481 gctttgaaaa aagcattagc cgttgaagaa cttacaacat tcgaaacggg agtaaaatat
     9541 caaatgtatc acgccttgtt tttattgttt atagggctga cttcccaact ttctgaaaaa
     9601 gcaaaaaaaa ccatttatta tttagttgtt actggagtca ttttcttttc gggttcgata
     9661 tacggattgg ctaccaataa actgagttcg tttgatttta aaaccattgg atttatcact
     9721 ccaattggcg gattgctgtt gattttggct tggggaattt tattggttaa tttactcaag
     9781 aaaaagtcat aactattctt attgtctatt taagaagttt ttaaaatata caaatacgtt
     9841 attcacttta aagaaagagt catgtttata gcatgactct ttttgtttta ccaaaaatag
     9901 ttctaacaac aaaatgtaac gttctccgtt aaaactttac taatcaatca tcaatcaaat
     9961 caatcacaat gttaaaatca ttttttattc tctgttctgg agccgacaaa accttgctag
    10021 aaggctgctc cgaaggcgaa caaaccaagt atgttggtat tggcgctact gttttcttta
    10081 ctgccgtgat ggctttcatt gccagttctt atgcactttt cacggttttc gacaatcctt
    10141 ttattgcaat gggatttgga atagtttggg ctttacttat ttttaatttg gatcgcttca
    10201 ttgtttccac tattcgaaaa agagaccgtt tcaagagtga attcttgcaa gctagtccac
    10261 gaattatttt agcgataatc attgctatcg tgatttctaa acctttggaa atcaaaattt
    10321 ttgaaaaaga aatcaatacc gttttattga aagagaaaaa cgcaatggct ttgaaaaaca
    10381 agaaggaagt cgccaattat ttcaaatcag atttagataa aaataaagcg gaaactgata
    10441 agttgaaagc agaaattgct ttaaaagaaa aagaagtaaa tacgctttat gaaacttaca
    10501 ttgccgaagc cgaaggaacc aaaggtacaa tgaaactggg gaaaggtcct gttttcaagg
    10561 aaaaaattgc caagcacaat ttggcttcag ccgaactaga ttccgtcaga aaaaccaact
    10621 tagcaaaaat tgctgtcaac gacaaaaaag caataacgtt gcaagctgat ttggacaaaa
    10681 aagttacaga aacccaaccc ataattgatg gatttgatgg tttgatggca agaatcaacg
    10741 ctttgaacaa actgccgttg attccttcgt tgtttattat gttgttgttt ttggccattg
    10801 aaacttcgcc aattatagcc aaattattgt cccaaaaagg cgaatacgac tataaactgg
    10861 aagacttgga aaccgctttg aaagtcactt tattgcaaga taaataccag cgtgatttgt
    10921 tggtaaaaac cagcgcagca atgcacgaca aagtctatga agatatcgcg caagacaaga
    10981 aattgtatga tttgcaacgt cagaaagcca ctgaattact ggaactccag gcgcataatt
    11041 ttgtggaaaa acaaaagaga actttataaa aaaacaaaac ccgacaaatc gaaacttgtc
    11101 gggtttgttg ttttaaaacg agatgattat tacatcaaac tcaaaatttc ttttttgaaa
    11161 gcgtcgtatt cgccttgtaa aatcaatccg ttgtcaagca attttttata gttctgaagt
    11221 ttagcaaaaa gctcgtcttg cgtcaattcg gttgttggtt tttcagttga agcagccgtg
    11281 ttttctacaa taggctctgc gtaattagca actgctggca taatttcggc ataatttacg
    11341 acttcttctg tttctacttc ttccacgatt tcttcgggaa tatattctgg aatttgtggt
    11401 tttgcttctt cagcaaccac gggatttttc agcaaatcca attgctcttt ggcgtaagta
    11461 gaaatttttc tggcctgaat ttttggaatg taatcaatag aaacttgaat ttgtgttttg
    11521 gtcgaaaacg aaaattcaga acccaaaatg ttttctttca taaaagttcc ttcaatttcg
    11581 tcccaagtaa aatcggtaaa attcattgag agtcccatat ttttgggttg gcaaatgatg
    11641 attcgtttgt tggttaccac gatactgtcc ggaaaaacgg taatggccgg ttttttttga
    11701 acagcgatat aaccaatttc ttcgtttttc atcaataaat cattcagttt tgaagtgatt
    11761 ttttcgatgg ctttagggtc ttgttcttcg ttcaagaatt tttttatatt gtcaatcatt
    11821 gtgtttttat ttgaatagtg ttctaagaat atttaacaaa agtaatgcct aattttctaa
    11881 ttgaagtatt tcggtttgaa tttttttggt caaacttttg aaaaccaaat cataggaatc
    11941 gtcaatccat tctttgacca atttatctgg aatttcgttg ttgatgacca aggtgttcca
    12001 atgcactttg ctcatatgaa atgctggttt gacatcgctg tattgggctc gcaattcttg
    12061 ggcataatcg ggattgcatt tcaaattgac agccggctct cctttttccc attgatttaa
    12121 agaggaaaga gcaaacattt ttcctcccac tttaaaaact aaagtatctt gatcgaacgg
    12181 aaaatgttcg gttgttcctt ttttagaaag acaatattcg tagtaagttt ctagattcat
    12241 atcgttactt tttccatcat tttttcggga tgtgccaatg ccttaaaacc gaatttctca
    12301 tacaaaaaat gcgcatcgga tgtggccaat cgccatattt taatattttt caaaactggt
    12361 tcgttcatca tcgcttcaat taaaatagag gaatagccgt ttcctcgatg ttcttcggca
    12421 ataaaaacat ccatcaaata ggcaaaaact acataatctg taaccactct ggcaaaacca
    12481 atttgaatat catccaaata aatcccgaaa caaaccgaac tgtcaatcgt ggtttgtact
    12541 tcgtcgatag ttcttcctgc cgcccaataa atatccttca aaaaatcttg aataaaagga
    12601 acattgagtt tagttttatc ggttgaaacg gtaatcataa atttatattt tatttaaaca
    12661 caaagccctg tgaactttgt gccttctttg tgctcttagt ggttaagact attccaaatt
    12721 taagtaaaac aaatcactgg caattccatc cgtcaaaaat tttccttttt gggttggttt
    12781 taaaatctta ttttcaatag aaagcaaatc atcatttata aatttatgtg cttgtttttg
    12841 caaataatct aaatattcat tgccgaattc atccaaaatt ctatccaagg atacacccca
    12901 aatggttcgt aaactcgtca taatatattc gttataacga tcggattttg ataaaatttc
    12961 aatttcgctg ggcaatttat tttcttccaa agatttcaaa taaatggaat tgttcgaaac
    13021 attccaactt cgggaaattc cgtcataact gtgtgccgaa ggcccaattc cgatgtattt
    13081 tttccctaac caatacgccg aattgttctt cgaaaaataa ttttccttgc caaaattcga
    13141 caattcataa tgaataaaac cattgttttc gagcgtttcg accaaaattt gaaaatgttc
    13201 ttcggccact tcgtctttgg gttgggcgat ttttccggtt tgaatgagtt tgttcaaagc
    13261 tgttttcggc tcgacagtca aagcataact cgaaatgtgt ggaatgccaa aactcaaagc
    13321 tgtttcgatg ttttgtttcc acttctcatt gttcattccc ggaattccat aaatcaaatc
    13381 tagggaaata ttgtcgaaat attttgttgc caattccaga gatttcttgg cttccaccga
    13441 attgtgtgcc cgattcatca tttgcaaatc atcttcgtaa aaagactgaa tcccgataga
    13501 aaggcgatta attccaattt ttgaaagttc gatcaaatac ccttccgaca aatcatcggg
    13561 attggcttcg agcgtgattt cggggttttc cgaaacttta taatttttat aaatcgaatc
    13621 caactgcaat ttcaaatcta caatctgcaa tctgctgggt gttcctccac cgaaataaat
    13681 cgtttctaca acctcatcct tgaactcgct tttacgcatt tgaatttctt tgaccaaagc
    13741 caaaaccatc tcgtctttct tcttcatcga agtcgaaaaa tgaaaatcgc agtaatgaca
    13801 cgcttgcttg cagaatggta tgtgtatgta gattccagac atattttgtt gttagcttaa
    13861 gttgattatt aaaatcattg gcaagagcgt actcaataac atgaaaacaa atccaaaaaa
    13921 tttatcagca aaaaggttca cattgcctga ataaaacaat tctcttggtg gaattttagg
    13981 atctcttttc tttcgtgccc aaaaaggaat taacattaat cgataaagca acaatgaata
    14041 taaaggacaa aaccaaaaca atataaaatt tggagaatca cttgtttcag aattcatttc
    14101 acataaagca agaattgaag ccaaaaatga gaagcttaat attattaaaa aagactttcg
    14161 ctctctcaat acttttctgc taattgtaaa aattaatgga ataaatatga aacctataaa
    14221 tgacaattct ttaaacatta taatcgattt ataaaaacct gaacactgaa cactacttct
    14281 taattctttc ttcattattc ttcacaaaag catcccaacc actataactt ttctcgccaa
    14341 taacttttcc ggaattgaaa aaatgacaaa ccgcagccgc caaaccatcg gtggaatcca
    14401 gattttttgg taattgtttc agacccaaaa gttgttggag cattttggct acttgttcct
    14461 tgctggcgtt tccgttgcca gtaatggcca tttttatttt ctttggttcg tattccgtga
    14521 tgggaatatc tctcgaaagt cccgctgcca ttgccacgcc ttgtgctctt ccgagtttca
    14581 acatcgattg gacgtttttg ccaaaaaaag gtgcttcaat cgcaatttca tcgggatggt
    14641 gtgtgtcgat taattctatg gtgcgttcaa aaatgatttt tagtttttgg taatggttgt
    14701 catatttgga caattgcaat tcattgagtt gcaaaaattc cattttcttg ttgacaattc
    14761 taatcaatcc aaaacccata atggtggttc cagggtcgat tcctaatatg atgcgttcgt
    14821 ttgtcaatgc gtaagtttaa aaatgttttg tcaagatttc tattccccaa caaagctaaa
    14881 caattccgtg tattttagtt caattataca gaaatgactt ttaccaaagt cgtaaaactt
    14941 tgataaaagt caaaaaagta tatggattaa aactattttt ttagctcagc aattactgag
    15001 tttggaactt tcaaaacagt accatggcgg gttccttgag gaaaactaac ttgttctgaa
    15061 atgtcttccc atccttcggc ggtttttttt acagcaccat attttttctg agtatattta
    15121 tcaaaataaa ctaaccattg accatttatt tggattgcgg ttggtccttc tgcccaatat
    15181 tttccagtga ttggttcact tgctttggtg tatggccctt ccaaattttt actggtggct
    15241 atttttaaat ttttctgaac cggaactttt gtttcatctt ttaaaaacat cgtatagcct
    15301 ttttcatctt ttacaataga agcatctatc acattaaaac ccggttcgta caataatttt
    15361 gtttttttga aacttttgaa atctttagtt gtggtataat aaattctatg attataccct
    15421 ttttcttctg ttgattgtgt ttcagtaaat ttcccttcaa tagtggaggc ccagtaaatc
    15481 atatagtttt tcgatttttc gtcataagta atttcgggag cccaagtgtt tcttgtcttt
    15541 ttttcatatt ccattactgg taaaaactct tgtttcgacc aatgaattaa atctttagaa
    15601 gaagcataac caatgccttt atcggtccaa ctaactgtcc aaaccatgtg gtacaaaccg
    15661 tctccgcctt ttataacaca aggatcacgc atcaatttgt ctttgcccac ttcgggagtc
    15721 aaaaaagaag cgtctttctt taaagcttcc catttataac cgtcttcact gtaagccaaa
    15781 tgcaatccgt cttctccgtt tcctttgaaa taagtaaaca catatgcatc agattttgga
    15841 ataaatccat tggaaatcaa ccatttttcg caggctgccg gccaattttt attggttcct
    15901 tgaacaccaa gaccaaaacc atgtccgcca ttttcataaa tgtgcatttc aactgggact
    15961 ttttggtttt ttagagccaa ataataattg agactatttt ctactggaac tgctttgtca
    16021 tcagtagcgt gaaccaaaaa tgtttttggt gtatttgcat cgacttgttt ttcgttcgaa
    16081 tatttagcta tcaattcact gctggcattt ttacccaaaa gattttcttg tgaacctttg
    16141 tgagtaattc cattttccat agaaattacg ggataaatca aaatagagaa atcaggacga
    16201 gcacttacat tatctttaga atcatacact ttgtcattat aacgtgttga cactgttgcg
    16261 gccaaatgtc ctcctgcgga aaagcctata acgcctattt ttgtaggatc aagattccat
    16321 tcatcggcac gtcggcgtag cgttcttatg gcttcttgcg catcttgaag tggaccaatg
    16381 gttttgtctt tcattatgat atcacttggc aaacggtatt taagaacaaa tgccgaaatt
    16441 ccaatagatt gaaaccatgc agccacttta tcaccttctt tttcgtgaga caaaagtgcg
    16501 tatcctccgc cagggcaaat aattaccgct gcatttttag catttttatt ggctgccaaa
    16561 aatattttca aagtgggttc ggtaactttt ctaattcctg ttatattacc ctgagcatcc
    16621 aatcgtggct cttgtttata atctgacgct tcgataactc ctggtatttt atcccataaa
    16681 ggaatttctt tgttttgaga aaaaacggga aggcttatcg ccaaaaataa aagaattaaa
    16741 acgaaactac tactgagatt tttcataaaa acatattcaa ttttatagta atctaaatga
    16801 ttttaaattt gactttatta gttcatttta taaatttcgg aacctgctag caagaaacat
    16861 cctaaaccat aatcctcgaa atcagggatt ttatcatacg atagcggttg tccatccttt
    16921 ggttctttcc ctgttgattg taaaaaacct aaaaatccat tttcgtgtaa actctctttt
    16981 gtcatggcgt tccaagcttt ttgaatcact ggcaaatagg tttctttttt caaaatacca
    17041 ctgtttacgc cataagctat tccgtaaacg aataaggctg ttcctgatgt ttctttttca
    17101 ccaaaattag ttgggtcgtg caaacttacg ttccaaaaac catctggacg ttgcacttta
    17161 actaatgctt ctgccatagt ttttaaatct ttaacatatt gctctctatg tggtgcattt
    17221 tctggaatta tagtcaaaac ttttgccaaa gcggcaatta cccagccatt tccacgactc
    17281 caataacagt cttcgccatt tggttctttg taaggaggat caaaatcggc atcgcgccac
    17341 cacaaaccat cttttgggtt gtaaagaccg tgatcgccgt gattatttct ggaatacata
    17401 tacatttggt acattttttc gaaatagcga ttgtcttttt ctaacactcc cattttggca
    17461 aaaacaggca ttcccatctg tatggcatca atccaagacc aatcgtcgtt ttgaggtgta
    17521 ttcaacaaca tattcaaagt agctcttgta ctttttaatt ttttagaatc gggttctaaa
    17581 ttgtacaaat cgatataggt ttgggctgca caataatcat cggcattgcg attggcactc
    17641 ccatttctaa aactccattt gtgaaactca gaccaatcat aagcatatct ataatattcc
    17701 tcttttggat agatttcgtg caaagccatt agcccttcat aataaacccc acgagtccaa
    17761 atattgctag gacgttcttt gttagtaact attgttttac tcacttcggg ccattttttc
    17821 ataaaatagt cattggcaag catcatttgt tttaacactt gttttttatc aaacaattgt
    17881 tgagcgtttg cgttattaaa attcaaaaag caaattgcaa aaaataaaaa cagttttttc
    17941 atcttataga atgattaatt agggatttaa ttttgtgatt ttaaaggagt gatagttact
    18001 ggtcctaata atccagaagg tgtaggtttc cattttgtgg cgtcaaattt attataatcc
    18061 ttgtcaacca tgttgatttc ataaaaaatc ttccactcct cacctttcag ttccatatct
    18121 cgaacccgat tagcgccaag attagtcact tgaactttta aagtgttttt tccttttttt
    18181 aattttccaa tgttcaattg gtaaggaacc gaccaagctg taccaataaa ttgatcattc
    18241 aaccacactt tggcgctttc gcgaacgtca ccaagattta aactccaatt ttctaccgta
    18301 ttgtttgtat tgtcaaattg caaagtataa gtggctgttc ctgaaaaagc ttcggcttcg
    18361 ggacttagtt ttgtccaaga ttctaaatta gaaacggttg ctgttgcagg caatttaggt
    18421 cctcctttat caaaagtaag ttgccatttt ccgtttaaag taattgcatt agcggttttt
    18481 tcaaaatagt tccatttttt ttgtgaagcg gttttttctg ttttcaagaa aagtgactga
    18541 cctggttcaa tttttagttt taccaaggtg tttttgtctg ttttggttac tacagcattg
    18601 ccatattctt tggtcaaagg gtcgaaaata actacatctt tattgccaac ttccaacgga
    18661 ataaaacctt caatgatttt ggcagaatga ttgactaaat aatacaattt ttcgccatca
    18721 actgttcgtc gagtatattt taagcccgaa ctaacaagag tttcaggata ggtttgcgct
    18781 tcttctaaag ctttgaaaat atccgaaaca ggctgaatag cagtcttatt ttcgtctaat
    18841 aatgccgcta atttttgatt ttgattttca aattcaaaat aacccggaac cgattttgga
    18901 agtccttcaa aaataatttt tcctcctgct ttttgcaaat cgattaattt ttgcaaagtt
    18961 gacaatggca ttttttcgca agcaggaata actattgatt tgaaagttcc gcctggtaaa
    19021 actatttttc cttctttgac ttgagcttga gcaatgaaat tatccgagat aaaatcgact
    19081 ccgtatccct tgcccatcaa attttttgtt gtgttataaa aagaggtgtc gtgtaaccat
    19141 tgtgaaaggg aatgtatttt gaattcaaaa aacaaagttc ctttttggta tttgtcccaa
    19201 gtatcaaaaa taggccaata gaccaaagtt tcgttgtcgg attgcccttg ttgcaacatc
    19261 gcttgacaat tggaaatata cgaaaacaaa gcaggcgcat cttcccaagt tggatctgta
    19321 ttattaaaat tggcggaagc ataaaatttc caccctggcc atttagctcg atctggcgaa
    19381 taggttgaac cgtgaagaaa aatatgattg ataccgttga gaagcaattc ttcggcttcg
    19441 ggtttgcatt gtgacaaagc gattttgaaa tggtcgcgca accaagtaaa agtttcagaa
    19501 gaaaccaaag gttttcctga aatatgtgcc gcagaagagg agaatttcaa cattactggg
    19561 tcggcatcgc cttcgcgaat atcttctttt tcacgtctaa aaccgggaat atcaaaaggc
    19621 atggaaccaa aagtttcgca ttcaggaata tccgccgaag cgtacaaatc gatcaaattt
    19681 ccgggtgaac cgtgggcctg aagtttggtt ttgaagtttt tcgaattggc ccaagttgtc
    19741 caaggtttgt caaacttatt caaaagtaaa tccgaaatag tttgtcggta atcgctttta
    19801 atgcggttgc caattgcatt atcggtttcg ctgagcaaaa gaggtaattt ttcttttaaa
    19861 tcataccctc ttctagtttt aaattcttcg aaaaaacgtg gggtaaaatc agttccatag
    19921 acttcataac tatcattaaa aatagctctt acttttcctt ctcgtccttt gaaagccgca
    19981 ttgaatggaa ctacataagc attaagtgct tcttctgaat aatgatctaa ggtatatcct
    20041 tttcctcctg gtgcagctct ttttacttgc tgacctgttt ttccgctaaa aaccgcataa
    20101 atagtataat cggttttttt agctttccat tgaagcacat ttccttttag ttcttgtgtc
    20161 aagtttacac acttaccatc actgctgtat gccattacat acaataggtc cgctggtatt
    20221 ttttctttgg ctttgtcaat ttctatagct ttgtcaaagg tttcgttttt cgtaagttgg
    20281 tatttttcta caatcaactt ggtggcggca taaggcaaag tcacgtgaga tcctccataa
    20341 ggccaacctg tacctaaaac catatcgact tgcatgtgca aactatccgc tacttgaacg
    20401 gaataatcca acatttccat ccattttgga gagaggtaat caataaaatt attttcttct
    20461 cctttcactc catatatagg agtgatttcg acaccgccaa ttccagcatt atgaaaatcc
    20521 tcaaggcttc ttttaagatt ggatttatca acggcactac ccatccacca ccaacgagcc
    20581 caaggctgat tgacattaga tgattttggc caaggcgaag gcgcagtatc ttgtgcaaat
    20641 gcacttaaag caaataaaaa tgcaacaata aataaggagt agtttttctt tagtctcatt
    20701 gtgtcttatg gtattctgtg attagttgta ataaagtgtt tctattaatc catctcagtc
    20761 aatatctaaa ttaatactta aatgtatcat ttatagaaat actagatact tttcttagaa
    20821 cgattgtaat cgagtaaaaa tataagaagt tcataaaaat agtatcctgt tgcgtcctgt
    20881 ttgttaaaat aaaaattaaa taactaatta ttagctggat ttatttcaga acagttaata
    20941 ctttcttatc aaaaaagttg ccataatcaa aatgaaagat ctaaaattag tgctggtgcg
    21001 ttaaaatatt attttaaatt atcaaaaata ttatattg
//
LOCUS       DAOSNN010000016        18107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3107_length_18107_cov_4.968314, whole genome shotgun sequence.
ACCESSION   DAOSNN010000016 DAOSNN010000000
VERSION     DAOSNN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3107_length_18107_cov_4.968314"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="PLQ70_01730"
     CDS             <1..1713
                     /locus_tag="PLQ70_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQE33364.1"
                     /translation="IMKNNTNKITILYIIVSIILAILYREFFKIIIPSNPLAFSFIND
                     LLFIVVSGILLRFVLHKNNIKNSEIYRKLKNTNNEIKESNERYDIVSKATSDIIWDWK
                     ILEDTMTWNKGIEAVFGYKQEEVGKSSKWWFDNIHPEDTIKMSIKLYSFIEQKTEKWQ
                     DQYRFKCADGTYKYVLDRGFILKDETGKSVRMIGAIQDITKQKEEEQRLKLLETVITH
                     TKDSIIITEANLNEGKIPDIVYINPAFSVISGYTSDEIIGKSPEIFIGPNSNPEDHNT
                     LIKAIKNKQECHIETLSYKKNNEPYWVNFNMLPVYNSDGELSHWVSIQRDVTEEKKQE
                     VEKEQLIKELTQNNKDLQQFSYITSHNLRAPLSNLTGLLNLIKDIPIENQELKEIIDG
                     FNKSTHLLNETINDLVRVIIIKDNPSIEKQSLLLKDIFEDVFSQLDFLIGLHKPIIKF
                     NFEEVTFLNTNKSYLESILLNLLTNSIKYKSETRKLKINISAHQIENSVILIFKDNGI
                     GIDLERNKDKIFGLYQRFHDYPDSKGLGLYLVKSQVETMGGTISIESKVNIGTTFTLT
                     FKNK"
     gene            1715..2119
                     /locus_tag="PLQ70_01735"
     CDS             1715..2119
                     /locus_tag="PLQ70_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQE33365.1"
                     /translation="MLNKILCIDDDQITLMLCKMVIAKATFSNEIATAKNGEDALNFF
                     DTLKTSDKTITKPELIFLDLNMPIMSGWEFLDAFNTEKYAEFHNTKIIILSSTIDPGD
                     LEKSKKYPLVIDFLPKPISKEMLEYLKDKIQF"
     gene            complement(2196..3218)
                     /locus_tag="PLQ70_01740"
     CDS             complement(2196..3218)
                     /locus_tag="PLQ70_01740"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HQE33366.1"
                     /translation="MYKQIIRPLFFCFDPEKVHYFTFSLIRNVSKIPGIPSLFRSLYV
                     VDDKRLETEVFGLKFKNPVGLAAGMDKGATLYNELSNLGFGFIEIGTITPKAQDGNPK
                     KRIFRLTEDNALINRMGFNNAGVQDAVERLKKNKGVLIGGNIGKNKVTPNEEATSDYL
                     ICFDALYDYVDYFVVNVSSPNTPNLRELQEKEPLKQLLQTLQNQNLTKSKQKPILLKI
                     APDLTDSQLLDIIDIVKETKIAGVIATNTTLSRVGLQSPNKVETGGMSGRPLRNRSTE
                     VIRFLSEKSNKAFPIIGVGGIHSADDAIEKLNAGASLIQLYTGFIYEGPALIKAINKK
                     ILEKMQ"
     gene            3379..4212
                     /gene="prmA"
                     /locus_tag="PLQ70_01745"
     CDS             3379..4212
                     /gene="prmA"
                     /locus_tag="PLQ70_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQE33367.1"
                     /translation="MSNIYLGYHFTVEPKELGSEILVAELGELPFESFIDTDFGIVAY
                     IQKDLWSEGILDDLFILKSPEFTISYEVQEIDQVNWNEEWEKNFEPIDVDGNCHVRAP
                     FHPKTKAEFDIVIEPKMSFGTGHHETTHMMIQHLLEIDVTGMKTLDMGCGTAILAILA
                     EMKGAQPIDAIDIDNWCYLNSIENSERNNCKHITVYEGDATLLKDKKYDLIIANINRN
                     ILLNDMQSYVDCLNPKGTLLLSGFYTEDIPFIDASCTEKGLTYVKKFERNNWVSLKFV
                     N"
     gene            4281..4556
                     /locus_tag="PLQ70_01750"
     CDS             4281..4556
                     /locus_tag="PLQ70_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HQE33368.1"
                     /translation="MSTIEKTRVKVSQKEATSINNEIIVYNDDVNTFDHVIETLIRVC
                     NHTPEQAEQCSLIVHYNGKCTVKTDVLEKLKPQCTQLLEAGLSAEII"
     gene            complement(4635..5048)
                     /locus_tag="PLQ70_01755"
     CDS             complement(4635..5048)
                     /locus_tag="PLQ70_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQE33369.1"
                     /translation="MKALKYIVVGFIFGIVLTKSEAVSWYRIYEMFMFQSFHMYGIIM
                     VAIVTGVIGIQIIKRKNIKDFKGFPIEIIPKEPGSTRFWVGGIFFGLGWALVGACPGP
                     IFILLGAGFLPLLLVLFGALFGTFLYGVIKDKLPH"
     gene            complement(5050..5607)
                     /locus_tag="PLQ70_01760"
     CDS             complement(5050..5607)
                     /locus_tag="PLQ70_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQE33370.1"
                     /translation="MEILFQTWPWYVSGFLIGMVMLSLIYFGKTFGMSSNLRSLCAMS
                     GLGKKVGFFDFDWKAQRWNLYVVLGAMLGGFVAVHFMSDASNVAINPKTIAQLTQLGI
                     DAPNGKLLPDALFSNAVFQSPKSIFILIIGGILIGFGTRYASGCTSGHAIYGLSSLQL
                     PSLKAVIGFFIGGLIMAHFILPLIF"
     gene            5767..6219
                     /locus_tag="PLQ70_01765"
     CDS             5767..6219
                     /locus_tag="PLQ70_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphooctonate aldolase"
                     /protein_id="HQE33371.1"
                     /translation="MKKTALLIALLISISSCTSTKSTLKNVDDNAPTPRLINNETFEI
                     KEYSKDKKYGYDKDYPINIFYGNTSNETINQQRFLNALAGPNGEQITYTKLESCCPFP
                     TKRSNMGAGFLDVYQLRWKGQKNPVTLYLNIYEKGVLMVPVGLSLKKK"
     gene            6274..7092
                     /gene="kdsA"
                     /locus_tag="PLQ70_01770"
     CDS             6274..7092
                     /gene="kdsA"
                     /locus_tag="PLQ70_01770"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQE33372.1"
                     /translation="MNILNIPQIKHTDSGNFFLLAGPCAIEGEEMAMKIAEKLVSITD
                     KLEIPFVFKGSFKKANRSRIDSFSGIGDEKALKILEKISKNFGVPTVTDIHTNEDAEI
                     AAQYVDVLQIPAFLVRQTDLVVAAANTGKVVNLKKGQFMSPESMKHAVQKVLDCNNQN
                     VMVTDRGTMFGYQDMIVDFRGIPTMQNYATTVLDVTHSLQQPNQTAGVTGGRPDMIET
                     IAKAGIAVGVDGIFIETHFDPANAKSDGANMLHLDYFEALMTKLVAIRKTINQF"
     gene            7104..7949
                     /locus_tag="PLQ70_01775"
     CDS             7104..7949
                     /locus_tag="PLQ70_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33373.1"
                     /translation="MKTSITFSALLFFCISLNSFAQNDTIAKERYTSHNKGKFTISWG
                     GNREKFTDSDIHFMGDDYNFVVQSATAHDKPKGWHVDYITPGRMTIPQTNFKLGYFFT
                     DKYSISIGVDHMKYVMTQNQTANITGIINGNPPFNGSYANTPTVMTEEFLMFEHTDGL
                     NYVYAEIARQDDISKWFGIKNTDKFQININGGFGFGALYPKTNTTLMGKERHDDFNVA
                     GYGTSIKAGINFTFFKYFTIEGDLKGGYINMPNIRTTKSTSDSAKQEFFFGETIIALG
                     GIFRL"
     gene            8089..8616
                     /gene="hemG"
                     /locus_tag="PLQ70_01780"
     CDS             8089..8616
                     /gene="hemG"
                     /locus_tag="PLQ70_01780"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HQE33374.1"
                     /translation="MKPKIGILYATVDGQTLKICRFIQDLLVEKGYTVALFTTENFDK
                     KVTDFDTFIIGASIRYGKHNPKIIEFINQNKSALEKVENAFFSVNLVARKPEKASPET
                     NPYFIKFLKEIDWIPKKSAVFAGKLDYQKYPFTDRLMIQLIMWMTKGPTNSKTKIEYT
                     DWGKVKEFANSIHIK"
     gene            8852..9556
                     /locus_tag="PLQ70_01785"
     CDS             8852..9556
                     /locus_tag="PLQ70_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HQE33375.1"
                     /translation="MDTINNFSYNDINKKNNIPKLFYQTNDLIFNEISEKLSDFEKAK
                     TIAIWLKKNIKGGPGLGKSSATALHKMIKGEGGVCSDFSQIFSNFCVINNIKVKEWGL
                     KSLSTDPKVSGGHSFNEVYCPEFQKWIMIDVAKSCLFYQSELDIPLSTIEYMQLKKEK
                     KEIIIRPLNGLDTTDNNLLNIFILSNSMPFIITNYDNKTVDYFLEKLDFFPVSIIHGL
                     LILLGKGYRFEFPENF"
     gene            9678..10925
                     /gene="pepT"
                     /locus_tag="PLQ70_01790"
     CDS             9678..10925
                     /gene="pepT"
                     /locus_tag="PLQ70_01790"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HQE33376.1"
                     /translation="MQHIIDRFISYVIIDTESDPNSETTPSTSKQWDLANKLVDELKT
                     IGMQDVTIDDKAYIMATLPSNVDHEVPTIGFIAHFDTTPDFTGAHVKPQIFPNYDGGD
                     ILLNADQNIILSPKYFKDLLQYKGQTLITTDGTTLLGADDKAGITEIVTAIEFLIKNP
                     EIKHGKIRVGFTPDEEIGRGAHHFDVEKFGCDWAYTMDGSQVGELEYENFNAAGAKIT
                     FKGKSVHPGYAKGKMINSMLIANDFINELPKGETPQETRGYEGFFHVHHLKGSIEETV
                     LELIIRDHNKKKFEKRKDLIEKITKKFNKKFAKQFGEDIVIAEVKDQYYNMKEKVLPV
                     KHIVDIAETAMKELKIKPIIKPIRGGTDGCQLSYKGLPCPNIFAGGHNFHGKYEYVPA
                     ESMQKAVDVIVRIAELTAKTIVK"
     gene            11088..11528
                     /locus_tag="PLQ70_01795"
     CDS             11088..11528
                     /locus_tag="PLQ70_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33377.1"
                     /translation="MEENKEEIVNETIKEVTTPKPKKPRTSRATKISQEVEKVEAVEE
                     ITQTVSDAEENQIEPFLEELDALIEKDEKKDKKKKKKMKLEKKKDKEKEKAKKAKAKE
                     KAKAKAKKAKKAKKEKAKKAKLKAKLKEKKAKAKAKKAKKNKKK"
     gene            complement(11600..11872)
                     /gene="yajC"
                     /locus_tag="PLQ70_01800"
     CDS             complement(11600..11872)
                     /gene="yajC"
                     /locus_tag="PLQ70_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQE33378.1"
                     /translation="MGQLTQFAPFLLMFVVIYFFMIRPQQKRAKQEKEFESALKVGDK
                     IITKSGLHGKIAEVAETTVVIETMAGKLKMERSAISMEMSAALAKK"
     gene            complement(11990..12901)
                     /gene="nusB"
                     /locus_tag="PLQ70_01805"
     CDS             complement(11990..12901)
                     /gene="nusB"
                     /locus_tag="PLQ70_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQE33379.1"
                     /translation="MQSIYAMHQNGSDNLEKEEKFLFYSIDAIQDLYLIMLSSLIEIC
                     KKESVFLHKSSQKHLATPQERKPNEKFIKNAIFQILAENNSLSIALETRKIDNWALND
                     DYILILLDAVKKSKLYANYMSNAVNTFEEDKEFIVNLFEQVIVPNEKLYDYLEDNKLT
                     WIDDIPVVNTEIIKQLRAIKAVEGGNFRVVKLFKDNEDKDFVRDLFRKTILNEKELAK
                     EYVDKTPNWDSERIAEIDTIILKMAICELLKFPSIPVKVTLNEYLELAKEYSTPKSSI
                     FINGILDNLVKELETNDRIKKIGRGLM"
     gene            13059..14816
                     /locus_tag="PLQ70_01810"
     CDS             13059..14816
                     /locus_tag="PLQ70_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE33380.1"
                     /translation="MKELQYLNKYFVKYKYSFLGIIITIVAQIFSLFTPKLISKSFTI
                     IESFAKDKTVTKAIIQHELVSNILLIIATTIIAGFLTFLMRQTLIVMSRHIEFDLKNE
                     VFKQYENLSQNFYKQNRTGDLMNRISEDVSKVRMYVGPAVMYTINTIIRFTIVIIYMI
                     NVSPRLTLYTLLPLPILSYAIFKISSEINKRSTIFQQYLSKVSSFTQEIFSGIRVIKA
                     YSLEDQHQNNMIDLSNESKHKSLSLAKVQALFGPLMMALIGISNLVVIYFGGLMYING
                     TIKSIGTIAEFILYVNMLTWPVASLGWVSSMVQEAEASQKRINEFLKIVPEIQNNTSK
                     KSIIEGAISFENVSYIYEDTNIKALQDISFTVHKGETLAILGKTGSGKSTIISLISRL
                     YDVSEGQITIDKKEISKVNLFDLRNSIGIVPQDAFLFSDTIKNNIKFGKEDATDDEIE
                     AVAKSAVVHDNIIGFSKQYDTILGERGITLSGGQKQRVSIARAIIKNPKILLFDDCLS
                     AVDTETEEAILNNLEEICRDKTTIIVSHRVSSAKNADRIIILDEGRIIEQGSHNQLLN
                     QEGYYAALYLKQLSEKELQ"
     gene            14887..15267
                     /locus_tag="PLQ70_01815"
     CDS             14887..15267
                     /locus_tag="PLQ70_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PUR family DNA/RNA-binding protein"
                     /protein_id="HQE33381.1"
                     /translation="MRENDMLEKEEIFSKVLRAGRRTYFFDVRATKADDYYITITESK
                     KFTETDGSFHFKKHKIYLYKEDFVAFAEILEEMTSYVLNHKGEEVISERHQKDYKKEY
                     DFDKIEEEKIPSISSFTNIEFEDI"
     gene            complement(15464..16396)
                     /locus_tag="PLQ70_01820"
     CDS             complement(15464..16396)
                     /locus_tag="PLQ70_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQE33382.1"
                     /translation="MENGIYAKFNTAKGSILVKLTHDLTPGTVGNFVALAEGNMENKI
                     KPQGQKFYDGLNFHRVIPNFMIQGGCPLGTGTGDPGYKFDDEFHSDLRHDAPGVLSMA
                     NSGPGSNGSQFFITHVATPWLDDKHTVFGHVIEGQDVVDAIAQGDKLETLEIIRVGAE
                     AKNWNAIEAYVGLKGARLKKLAALKAESEAKMEKLAAGFEKTESGLRYQFIQRGNGKQ
                     AEAGKTVSVHYEGSLENGKVFDSSYPRKKPIEFKLGIGQVIEGWDEGIALLKVGDKAR
                     FVIPSDLGYGPNGAGGVIPPNAILIFDVELMDVK"
     gene            complement(16483..16842)
                     /locus_tag="PLQ70_01825"
     CDS             complement(16483..16842)
                     /locus_tag="PLQ70_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010137028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQE33383.1"
                     /translation="MQSFGASKEFIKDDEFDWEAVGKGIKRKIMGYDDTIMMVNVLFE
                     KGAIGTPHEHYHSQVTNVAKGAFEVTINGVTKVMKQGDCFYIPPHIIHGVVCIEDGLL
                     IDVFSPIREDFMQKMTY"
     gene            17044..17718
                     /gene="trmD"
                     /locus_tag="PLQ70_01830"
     CDS             17044..17718
                     /gene="trmD"
                     /locus_tag="PLQ70_01830"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQE33384.1"
                     /translation="MRIDIITVLPELLRSPFEASIMKRAIDKGIVEVHFHNLRDYTTN
                     KQKSVDDYPYGGGAGMVMNIQPIDDCITHLKSERTYDEIIYMSPDGDTLNQKMANTMS
                     MYENIIILCGHYKGVDQRVRDHFITKEISIGDYVLSGGELGALVLCDSIIRLIPGVLS
                     DETSALTDSFQDNLLSGPIYTRPADYKGWKVPEVLTSGNFAKIDKWREDMAYEHTKNR
                     RPDLLE"
     gene            17905..>18107
                     /locus_tag="PLQ70_01835"
     CDS             17905..>18107
                     /locus_tag="PLQ70_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQE33385.1"
                     /translation="MADLLKFVQDEFVTRKDFPEFGAGDTITVFYEIKEGEKTRTQFF
                     KGVVIQRRGSGNTETFTIRKMSGA"
BASE COUNT         6682 a         3094 c         2754 g         5577 t
ORIGIN      
        1 ataatgaaaa ataacaccaa caaaataacc attctctata taatagtatc cattattctg
       61 gctattctat accgcgaatt ttttaaaata ataattccat caaatccact tgctttcagt
      121 tttataaatg accttctatt tatagtggtt tctggcatac ttcttaggtt tgttttgcat
      181 aaaaacaaca ttaaaaactc cgagatttac agaaaattaa aaaatacaaa taatgagatc
      241 aaagagtcta atgaaagata tgatatcgta tcaaaagcta ctagtgatat catttgggat
      301 tggaaaattt tagaagacac catgacctgg aacaaaggaa tcgaagctgt ctttggatac
      361 aaacaagaag aagtaggcaa aagttcaaaa tggtggtttg ataatataca cccagaagac
      421 accatcaaaa tgtctattaa attatattct tttattgaac aaaaaaccga aaaatggcaa
      481 gatcaatatc gctttaaatg tgcagatggc acttataagt acgttttaga tagaggcttt
      541 attttaaaag atgaaaccgg aaaatcagta cgaatgatag gagccattca agatattacc
      601 aaacaaaaag aagaagaaca gcgactaaag ctgttagaaa ccgttattac acatacaaaa
      661 gactctataa ttattaccga agccaatttg aatgaaggta aaatacccga cattgtttat
      721 ataaatcccg ctttttctgt catctcagga tacacttcag acgaaattat tggaaaatct
      781 cctgaaattt ttataggtcc aaattcaaat ccagaagacc ataatactct aataaaagct
      841 ataaaaaata agcaagaatg ccatattgaa acactaagtt acaagaaaaa caatgaaccc
      901 tattgggtta atttcaatat gctgccagta tataattctg atggcgaact ttcacactgg
      961 gtttcaatac aaagagatgt cacagaagaa aaaaaacagg aagtagaaaa agaacaatta
     1021 attaaggaat taacccagaa taataaagat ttacagcaat tttcatacat cacctcacac
     1081 aatcttaggg ctcctctgtc aaatttaact gggttactaa acttaattaa agatatccct
     1141 attgaaaatc aagagcttaa agaaattatt gacggcttta acaaatcaac tcatttatta
     1201 aacgaaacca taaatgattt agtaagagta attatcatca aagacaaccc ttcgatcgaa
     1261 aaacaatcgc tattgctaaa agacattttt gaagacgtat ttagtcaatt ggattttcta
     1321 attggattac ataaacctat tataaaattc aattttgaag aagtaacttt cctcaatacc
     1381 aataaatctt atttagaaag tattctatta aacctattga ccaattcaat taaatacaaa
     1441 tctgaaactc gaaaattaaa aataaacatt tctgcacatc aaatagaaaa tagtgttatt
     1501 ttaatcttca aggataatgg aataggtatt gatttagaaa gaaataaaga taaaattttc
     1561 ggactatatc aaagatttca tgattaccct gatagcaaag gattaggact ttacctagta
     1621 aaatcacaag ttgaaactat gggaggcaca attagtatag aaagcaaagt aaatattgga
     1681 actacattta cattaacttt taaaaacaaa taaaatgctc aataagattt tatgcattga
     1741 tgacgatcag attacgctaa tgctatgcaa aatggtaatt gcaaaagcta ctttttcaaa
     1801 tgaaatagcc acagcaaaaa atggagaaga tgcccttaat ttctttgaca cactcaaaac
     1861 atcagataaa acaattacaa aaccagaatt aatttttttg gatttgaaca tgcctattat
     1921 gagtggttgg gagtttttag atgcctttaa taccgaaaaa tatgcagagt ttcataacac
     1981 caaaataatt atcctctcct caacaataga tcctggagat ttagaaaaat caaaaaaata
     2041 cccactagta attgattttt tacccaaacc aatctcaaaa gaaatgcttg aatatttaaa
     2101 agataaaatt caattttaat tcatttcttg caaaaaaact atttttgaga ccacatttac
     2161 cgatttatta attggtcttt tgagacacct ttcgattact gcattttctc taaaatcttc
     2221 ttattgattg cttttattaa tgcagggcct tcataaataa atcctgtata aagttggatt
     2281 aaacttgctc ctgcattcaa tttttcgata gcatcatcgg cagaatgtat tcctcctact
     2341 ccaataatag gaaaggcttt attgcttttt tcggaaagaa aacgaatcac ttcagtcgaa
     2401 cgatttcgca atggtcttcc cgacattcct cctgtttcta ctttgtttgg tgattgcaat
     2461 cctacacgag ataaagtggt attagtagca attacacctg caattttggt ttctttaaca
     2521 atatcaataa tatctaacaa ttgcgaatcg gtcaaatcgg gagcaatttt caaaagaatt
     2581 ggtttttgct ttgactttgt taaattctga ttttgcaagg tttgcaacaa ttgtttcaac
     2641 ggctcttttt cctgcaattc tcgtagattg ggtgtgttgg gcgaactcac atttaccaca
     2701 aaataatcga cataatcata taaagcatca aagcaaatga ggtaatccga agtagcctct
     2761 tcattaggag ttactttatt tttgccaatg tttccaccaa taagaacgcc tttgtttttc
     2821 tttaatcttt caactgcatc ctgaacacct gcattattga aacccattct attaataagt
     2881 gcattgtctt ctgttaggcg aaaaatacgc tttttaggat ttccatcttg cgcttttggt
     2941 gtaatggttc caatttcaat aaatccaaaa cctagattag acaattcgtt atacaaagta
     3001 gcgcctttat ccattcctgc tgctaatcca acggggtttt taaactttaa gccaaagact
     3061 tcggtttcta atcgtttgtc atctacaaca tataaagatc taaaaagtga tggaattcct
     3121 ggtattttgg atacatttcg aattaatgaa aaagtaaaat aatgcacttt ttcgggatca
     3181 aaacaaaaaa atagaggacg gataatttgc ttgtacataa tataataagt tgtggtgcaa
     3241 aaatagaatt atcttatcga attataaata tttatacgaa attaattttc caacaaccta
     3301 tttattggtg accaaaactg caaactgacc tctgttcact gccaactttt ttattacctt
     3361 tgcaaaaaaa taaattttat gtcaaacatc tatttaggct accattttac agttgaaccc
     3421 aaagaactag gttcagaaat tttggttgcc gaacttggcg agttaccttt cgaaagtttt
     3481 atcgatactg attttggtat tgttgcttat attcaaaaag atctttggtc agaaggaatt
     3541 ctagacgatt tgttcatttt aaaatcgcca gaatttacta tttcttatga agtacaagaa
     3601 attgatcaag taaattggaa cgaagaatgg gaaaagaatt ttgagccgat tgatgtagat
     3661 ggaaactgcc acgttcgcgc accatttcat cccaaaacca aagccgaatt tgatattgtt
     3721 atagaaccca aaatgagttt cggaactggt catcacgaaa ccacacatat gatgattcag
     3781 catttattag aaattgatgt cactggaatg aagacgctgg atatgggttg cggaacagcc
     3841 attttagcca ttttagccga aatgaaaggc gctcaaccta ttgatgctat cgatattgac
     3901 aattggtgtt atttaaattc tatcgaaaat tcagaacgca ataattgcaa acacattact
     3961 gtttatgaag gtgatgctac cttgttgaaa gacaaaaaat acgatttaat tatagctaac
     4021 atcaaccgaa atattttgtt gaatgatatg caaagctatg tagattgtct aaatccaaaa
     4081 ggaactttgc tactgagtgg attttacacc gaagacattc cgtttataga cgcttcttgt
     4141 actgaaaaag gattaacata cgttaaaaaa tttgaaagaa acaattgggt atctttaaaa
     4201 tttgtaaatt agtgcactaa aatcgaaaaa attaaacaaa gccagcatta tttttattta
     4261 taacctacaa ttatttaaaa atgagtacta tagaaaaaac aagagtaaaa gtcagccaga
     4321 aagaagcaac ttcaataaac aacgaaatta tagtatataa tgacgatgta aacacttttg
     4381 atcacgttat cgaaacctta attagagttt gcaaccacac tcccgaacaa gcagaacaat
     4441 gctcattaat agttcattat aacggaaaat gtacggtaaa aacagatgtt ttagaaaaat
     4501 tgaaaccaca atgcacccaa ttactggaag caggtttgag tgctgaaata atttaattgt
     4561 ttagtttaat aatcataaaa aaaacaggat tttcaaaaag agaatcctgt ttttttttat
     4621 cacttttctt gagtttaatg aggtaattta tcttttatca ctccatataa aaacgttcca
     4681 aaaagtgctc caaagagaac cagcaacaaa ggcaaaaatc cagctcctag aagtataaat
     4741 ataggcccag gacaagctcc tactaatgcc cagccaagcc caaagaaaat tcctcctacc
     4801 caaaatcgag ttgaaccagg ttcttttggg ataatttcaa ttggaaaacc tttaaaatct
     4861 tttatatttt ttcttttaat aatttggatt ccaatgacac ctgtaacaat cgcaaccata
     4921 attattccat acatatgaaa agattgaaac atgaacattt cataaattcg ataccaagaa
     4981 accgcctctg attttgtcaa aacaattcca aaaataaatc caacaactat atattttaat
     5041 gctttcatac taaaaaatta aaggtaaaat aaaatgtgcc attattaacc ctccaataaa
     5101 gaatccaatc acagctttca aggacggtaa ttgcaaacta ctcaaaccat aaatagcgtg
     5161 tcccgaagtg catccgctag cataacgtgt accaaaacca attagaatac ctccaattat
     5221 taaaataaaa atgctttttg gagactgaaa aacagcattt gaaaacaagg catctggcaa
     5281 taattttccg tttggagcgt caattcctag ttgcgtcaat tgtgcaatcg ttttgggatt
     5341 aatagccaca ttagaagcat cactcataaa atgaaccgca acaaaaccac ctaacattgc
     5401 gcctaaaacc acatataaat tccaacgctg tgctttccaa tcaaagtcaa aaaaacccac
     5461 tttttttcct aaacccgaca ttgcacataa cgaacgtaag ttagaggaca tcccaaaagt
     5521 ctttccaaaa taaattaaac ttagcattac cattcctatc aaaaaccccg aaacatacca
     5581 aggccaagtt tgaaataaaa tctccataat attgtattta tttggctaaa ataataattc
     5641 taattttatt aaaatgaata attaaggcat aattgttgtt tatgctagca atacctcata
     5701 aatttgcaat taaattacac tgcatttatt tgacaacaaa atcctataaa tttctatagt
     5761 aagattatga aaaaaacagc tcttcttatc gctctactaa ttagcatcag ttcatgcaca
     5821 agtacaaaat cgacccttaa aaatgtagat gacaatgctc caacaccaag attaataaat
     5881 aatgaaactt ttgagattaa ggagtacagc aaagacaaaa aatacggata cgataaagat
     5941 tatcctatca atatttttta tggaaacact agcaacgaaa cgataaatca acaacgattc
     6001 ttgaatgcgt tggctggccc aaatggcgaa caaatcacct atacaaaact tgaaagttgc
     6061 tgcccttttc cgacaaaaag aagcaatatg ggtgctggtt ttcttgatgt gtaccaatta
     6121 agatggaaag gacaaaaaaa tcctgtaaca ttatatttaa atatttacga aaaaggagtg
     6181 ctaatggttc ctgtaggact tagtttgaaa aagaaataat ttgatttcaa aaccataaat
     6241 cataactatc tttgccttct gaaaaaccac actatgaaca tactcaatat tccacaaata
     6301 aaacatacag acagcggaaa cttctttcta ctagcaggac cttgcgccat tgaaggtgaa
     6361 gaaatggcga tgaaaattgc cgaaaaatta gtttcaatta ctgacaaact agaaattcct
     6421 tttgttttta aagggtcatt caaaaaagcc aatcgctcta gaattgatag cttttcggga
     6481 attggagacg aaaaagcact aaaaatactt gaaaaaatat ctaaaaattt tggtgttcct
     6541 actgtaactg atattcacac taatgaagat gccgaaatag cagcccaata tgttgatgta
     6601 ttgcaaattc cagctttctt agtgcgccaa accgatttgg ttgtggctgc tgccaatact
     6661 ggaaaagtgg tcaacctaaa aaaaggacaa tttatgagtc ctgaaagcat gaaacatgcg
     6721 gttcaaaaag tattggattg caacaaccaa aatgtgatgg taaccgatag aggaaccatg
     6781 tttggctacc aagatatgat tgtagatttc agaggaatac caaccatgca aaactatgct
     6841 actaccgttc tggatgtgac tcactccttg caacaaccca atcaaactgc tggagttact
     6901 ggcggaagac ccgatatgat tgaaaccatt gccaaagccg gaatcgcagt cggtgtagat
     6961 ggtattttta ttgaaactca ttttgaccct gctaatgcta aaagtgatgg tgccaatatg
     7021 cttcatctag actatttcga agctttaatg accaaactag tcgccatccg aaaaaccata
     7081 aatcaatttt aaattaatct attttgaaaa cttcaattac attttcagct ttattatttt
     7141 tttgtatttc cctgaactct ttcgctcaaa acgacaccat cgcaaaagaa agatatactt
     7201 ctcataacaa gggaaaattc actatttcct ggggtggaaa tagagaaaaa tttaccgact
     7261 ctgacattca cttcatgggc gatgattaca attttgtagt ccaaagtgca acagctcacg
     7321 acaaaccaaa aggatggcat gtggattaca tcacacctgg tcgaatgaca attcctcaga
     7381 ccaattttaa attaggctac ttttttaccg acaaatacag tatttctatt ggagttgacc
     7441 atatgaaata tgtaatgact caaaatcaga cggctaatat tacaggaata ataaacggca
     7501 atcctccttt caatgggagt tatgcaaaca caccaacggt gatgacagaa gagtttctaa
     7561 tgtttgaaca caccgatggt ttaaattatg tatatgcaga aattgcccgt caagatgaca
     7621 tctcaaaatg gtttggcata aaaaacaccg ataaatttca gataaacatc aatggtggct
     7681 ttggttttgg tgccttatat ccaaaaacca acacaacact tatgggcaaa gaaagacacg
     7741 atgattttaa tgtagctggt tatggcactt ctataaaagc gggaatcaac tttacatttt
     7801 tcaaatattt cacaatcgaa ggcgatttga aaggtggtta tattaatatg cccaatatta
     7861 gaacaactaa aagtacttct gatagtgcta agcaagaatt tttctttgga gaaaccatca
     7921 ttgctcttgg cggaattttt agattataaa tctatttcaa tacaaattaa aatctcctaa
     7981 ttaggagatt tttttgttta taccaacttt aaattcaaat aattagttgc attctaatca
     8041 gttttacaaa gattaattct taaattagta cacaaaaatt tcgactatat gaaaccgaaa
     8101 attggaattc tatacgctac cgtagatggt caaactctga aaatttgcag gtttattcaa
     8161 gatcttttag tcgaaaaagg atacactgta gcacttttta ctactgaaaa tttcgacaaa
     8221 aaagtaaccg atttcgatac gtttattatt ggcgctagca ttcgctatgg caaacacaat
     8281 ccaaaaataa tcgaattcat aaaccaaaat aaaagtgcac tcgaaaaagt agaaaatgcc
     8341 tttttttcag tcaatcttgt ggcaagaaaa cctgaaaaag ctagcccaga aaccaatccc
     8401 tattttatta aattcttgaa agaaatcgat tggattccaa aaaaatcggc tgtttttgct
     8461 ggaaaattag actatcaaaa atatcccttt acagacagat taatgataca attgataatg
     8521 tggatgacaa aaggccctac aaattcaaaa accaaaatcg aatacaccga ttggggaaaa
     8581 gtcaaggaat ttgcaaattc aattcatatc aaataacatt tggtcgatta ttttttcctt
     8641 ttaaagcttt tttagtagtt tgcagataat ttttgtatta ctttagaaaa ttataaaaca
     8701 ctataatttc aaaatcaatt atattgcctt tttcgataca aaaagcagta taaatttatc
     8761 ttctaatatg agaaaacctc taaattggta cttaaaaaga caccccttat tatataaaat
     8821 ccgttttaga ttaatatcta aaaatgtctc tttggatact atcaacaact tttcttataa
     8881 tgatatcaat aaaaaaaata atattcccaa actattttac caaacaaatg acttaatatt
     8941 taacgaaata agtgagaaat taagcgattt tgaaaaagcc aaaacaatag ctatttggct
     9001 aaaaaagaac ataaaaggtg gaccaggatt aggcaaatca tcggcaacag ctttacacaa
     9061 aatgataaaa ggtgaaggag gggtttgcag cgatttttcc caaatttttt caaatttttg
     9121 cgttataaac aatataaaag taaaagaatg gggattaaaa agcctatcga ctgacccaaa
     9181 agtctcggga ggacattcgt ttaatgaagt ttactgccct gaatttcaaa aatggattat
     9241 gatagacgta gcaaagtcct gcttatttta tcaatccgaa ctagacattc ctttgtcaac
     9301 aatagaatat atgcaactga aaaaagaaaa aaaagaaatt atcattagac ctttaaacgg
     9361 acttgacact accgacaaca accttttaaa tatattcata ctctccaatt caatgccttt
     9421 tatcattacc aattatgata ataaaaccgt cgattatttc cttgagaaac tagatttttt
     9481 tccagtatca atcattcatg gattacttat ccttttaggc aaaggatatc gatttgaatt
     9541 tcctgagaat ttttaaaccc atttatttta tcttataaaa cttgaatcgg tttcttttta
     9601 aaatcaaaaa ctcatctttg attaactttt cattgcacaa tcattcggat ttgtatcttt
     9661 gttgaaaata caatcttatg caacatatta tagatcgctt tataagctac gtcatcattg
     9721 ataccgaatc tgaccctaat tctgaaacta ctcctagcac ctcaaaacaa tgggatttag
     9781 ccaacaaatt ggtcgatgaa ctaaaaacaa ttggaatgca agacgttaca attgacgata
     9841 aagcctacat catggcaact ttaccatcaa atgtagatca cgaagtgcca actattggat
     9901 ttattgcaca ctttgacacc acacccgatt ttacaggagc ccatgtaaaa cctcaaattt
     9961 tccccaatta tgatggtggc gatattctat taaatgctga ccaaaacatt attttatcac
    10021 ccaaatattt caaggattta ttacaataca aaggacaaac cttaattact accgacggaa
    10081 ccacactcct tggagccgat gataaagctg gaattaccga aattgtaaca gcaatagaat
    10141 ttctaatcaa aaaccctgaa atcaaacacg gaaaaattag agttggattt actcctgacg
    10201 aagaaattgg acgtggagca catcattttg acgtagaaaa atttggctgc gattgggctt
    10261 acacaatgga cggaagccaa gtaggcgaac tggaatacga aaatttcaat gccgctggag
    10321 ctaaaattac tttcaaagga aaaagtgttc atccgggtta tgccaaagga aaaatgatca
    10381 attctatgct tatcgccaat gatttcatta acgaattacc aaaaggagaa acacctcaag
    10441 aaacaagagg gtatgaaggt ttttttcatg tgcatcacct aaaagggagc attgaagaaa
    10501 cggttttgga acttataatt cgagaccata acaagaaaaa atttgaaaaa cgaaaagatt
    10561 taattgaaaa aattaccaaa aaattcaata aaaaattcgc aaaacaattt ggcgaagaca
    10621 tagttattgc cgaagtaaaa gaccaatact acaatatgaa agaaaaggta ttgcctgtaa
    10681 aacatattgt agatattgcc gaaacagcaa tgaaagaatt gaagataaaa ccaataataa
    10741 aacccatccg tggcggaaca gatggctgcc aattatccta caaaggcttg ccttgcccaa
    10801 atatttttgc aggtggtcac aattttcacg gaaaatacga atatgttcct gctgaaagta
    10861 tgcaaaaagc agtagatgtg attgtaagaa ttgccgaatt gacagctaaa actattgtca
    10921 agtaatagta ttataaattc aactgaatta ccaaaaagcc atcaatttga aggctttttt
    10981 tgaataaata attaaggaaa aacttaacat taagaaattg atttaatgat tatctttgat
    11041 tacaattaac ttatccttaa aaaaatcaat cctaaaaaat ataaattatg gaagaaaaca
    11101 aagaagaaat tgtaaacgaa acaatcaaag aagtaactac tcccaaacct aaaaaaccta
    11161 gaactagcag agcaactaaa ataagtcaag aagttgaaaa agttgaagca gttgaagaaa
    11221 ttacacaaac tgtatctgat gcagaagaaa atcaaataga acccttttta gaagaattgg
    11281 atgcactaat tgaaaaagat gaaaaaaagg acaagaaaaa gaaaaaaaag atgaaactag
    11341 aaaagaaaaa agacaaggaa aaagaaaaag ctaaaaaagc taaagctaaa gaaaaagcaa
    11401 aggctaaagc taaaaaagcg aagaaggcta aaaaagagaa agccaaaaaa gccaaactca
    11461 aagctaaact caaagagaaa aaagccaaag ctaaagctaa aaaagcaaag aaaaataaaa
    11521 agaaataatt gttttaaaat aaaaaatccc aaattccaat tagaatcgga atttgggatt
    11581 tttttattgt agataaaaac tattttttag ccaaagctgc actcatttcc atagaaatgg
    11641 cagaacgctc catttttagt tttccagcca tcgtttcgat aacaactgta gtttcagcaa
    11701 cctcagcaat ttttccgtga agaccacttt tggtgatgat tttgtcgcct actttcaatg
    11761 cactttcaaa ttctttttct tgtttggctc ttttttgttg tggtcgaatc ataaagaaat
    11821 agattaccac aaacattaat aaaaacggcg caaattgagt taattgtccc ataattttaa
    11881 attatttttt tgtttgatta ttattgattt tgtttagatt taagcaattg aattatttat
    11941 tttgatgaaa tatttacaaa catcgtaatt cgtaattctc aattaatttt tacattaatc
    12001 cgcgtccgat ttttttgata cgatcgttag tttctaattc tttgaccaaa ttatctaaaa
    12061 ttccgttaat aaaaatacta ctttttggtg ttgaatattc tttggctaat tctaggtatt
    12121 cgttcaaagt aacttttaca ggaatcgaag ggaatttcaa caattcacaa atagccattt
    12181 taaggataat ggtatcaatt tcggcaattc tttcgctgtc ccaattggga gttttatcta
    12241 catattcttt tgccaattcc ttttcgttca aaattgtttt tcggaataaa tctctaacaa
    12301 aatccttgtc ttcgttgtct ttgaataatt ttacaactct aaaattaccg ccttcaacag
    12361 cttttattgc tcttaattgc ttaataattt cagtatttac aactggaata tcatcaatcc
    12421 aagttagttt attgtcttct agatagtcgt ataatttttc atttggaaca ataacctgct
    12481 caaacaaatt gacaataaat tccttatctt cttcgaatgt gttaacggca ttgctcatat
    12541 aatttgcata caacttgctc tttttaacag catccaaaag aattaaaata taatcatcat
    12601 tcaaagccca attgtctatt ttacgcgttt ctagagcaat actcaacgaa ttattttcgg
    12661 caagtatttg aaaaatagcg ttttttataa atttttcgtt gggtttgcgc tcttgcggag
    12721 tggcaagatg tttttggcta gacttatgca aaaaaacaga ttctttcttg caaatttcga
    12781 tcaatgagga aagcattata aggtataaat cctgaatagc gtctatacta taaaaaagga
    12841 atttttcttc tttttctaaa ttatctgacc cattttgatg catcgcatat atggattgca
    12901 taaccttaac gcgaatatgt cttctattta ccaccttgta agaacttttt taaattagtt
    12961 tgcaaaaata agaatttcat tttttaaaac agaattaaac ctgaaaaata tctaattttc
    13021 ctattacctt tgaaattcat aaaagaagaa aaatccagat gaaagaatta caatatttaa
    13081 acaaatattt tgtaaaatac aaatacagct ttttaggcat cattattact atagttgcac
    13141 aaattttttc tttgtttacg cctaaattaa taagcaaatc gtttaccata atcgaatcat
    13201 ttgcaaaaga taaaacagtt acaaaagcaa tcattcaaca cgaattagtt tctaatattt
    13261 tattgataat tgcgacaaca ataattgctg gtttccttac tttcctaatg cgtcaaacac
    13321 taattgtaat gtcgcgtcac atcgaatttg atttaaaaaa tgaagttttc aaacaatacg
    13381 aaaatctatc gcaaaatttc tacaaacaaa accgaactgg cgacttgatg aaccgaatta
    13441 gcgaagacgt ttcgaaagtg agaatgtatg ttggtccagc cgtaatgtac accataaata
    13501 ccattattcg ttttacaatt gtaatcattt atatgattaa tgtttcgcca cgattgactt
    13561 tgtacacctt gcttccattg ccaattttgt catatgctat attcaaaata agttctgaaa
    13621 tcaataaaag aagcactatt tttcaacaat atttatctaa agtttcgagt ttcactcaag
    13681 aaatattttc aggaattaga gtcataaaag cctattcatt agaagaccaa catcaaaata
    13741 acatgattga tttgtctaac gaaagtaaac acaaaagctt aagtttagca aaagtacaag
    13801 ccttatttgg cccattaatg atggctctga tcggaataag taacttagtt gtgatttatt
    13861 ttggtggatt aatgtacatc aacggaacca tcaaaagcat tggaaccatc gccgaattta
    13921 tcctgtatgt aaatatgttg acttggccag tggcttcatt aggttgggtt tcatcaatgg
    13981 ttcaagaagc cgaagcctcg caaaaacgca tcaacgaatt cctaaaaatc gttcctgaaa
    14041 tacaaaataa tacctctaaa aaatcgataa ttgaaggggc aatttctttc gaaaatgtta
    14101 gctatattta tgaagatacc aacataaaag ctttgcaaga catcagcttc acggttcata
    14161 aaggagaaac attggctatt ttaggaaaaa cgggttcagg aaaatcaact attatttcac
    14221 taatttctag actttatgac gtatcagaag gtcaaattac tattgacaaa aaagaaatta
    14281 gtaaagtcaa tttattcgat ttaagaaaca gcattggaat tgtgccacaa gatgcttttt
    14341 tgttttcaga caccatcaaa aacaacatca aatttggcaa agaagacgct accgacgatg
    14401 aaatagaagc tgttgcaaaa agtgcagtag ttcacgacaa tatcatcggt ttcagcaaac
    14461 aatatgatac cattctaggc gaaagaggta tcacactttc aggtggacaa aaacaacggg
    14521 tttcgattgc gagagccatc atcaaaaatc ctaaaatttt attattcgac gattgtctat
    14581 ctgcggttga taccgaaacc gaagaagcca ttttgaacaa tctcgaggaa atttgtagag
    14641 acaaaacgac cataattgtg agccatagag tatcttcggc caaaaatgca gaccgtatta
    14701 ttatccttga cgaaggacga attattgagc aaggctctca taatcaatta ctaaatcagg
    14761 aggggtatta tgccgcttta taccttaaac aactttctga aaaagaattg caataattgt
    14821 tgtgtaatac atatattttt tagatttttg agtagtactt aacaaccaca aattgaccga
    14881 aggattatga gagaaaatga tatgttagaa aaagaagaga ttttttctaa agttttaaga
    14941 gctggaagaa gaacctattt ctttgatgta agagctacaa aagcagacga ttattatatt
    15001 acaattaccg aaagcaagaa atttaccgaa accgatggtt catttcactt caaaaaacac
    15061 aaaatatatt tatacaaaga agattttgta gctttcgccg aaattttaga agaaatgact
    15121 tcttacgttt tgaaccacaa aggtgaagaa gtaatctctg aaagacacca aaaagattat
    15181 aaaaaagaat atgattttga caaaatcgaa gaagaaaaaa ttccttctat ttctagtttt
    15241 accaatatcg aatttgaaga tatttagatt ttaattcaat aaaccaaata ataactaacc
    15301 aaacccaatc accaagtcca aaagtcgcaa gatttttgga cttttttttt gcaatgtatc
    15361 tccaaaaaca tctaaataaa aaatcccaaa actctcgctt tgggattaga tatatttatg
    15421 caaaacagta cagacgttgc aatgcaatgt ctctatcgtt attttatttt acatccatta
    15481 attctacatc aaaaattaag attgcatttg gcggaataac tcctccagca ccatttggtc
    15541 catatcctaa atcagatgga ataacaaaac gagctttatc tcctactttc aacaaagcaa
    15601 ttccttcgtc ccaaccttca ataacttgac cgattcctaa tttgaattcg attggttttt
    15661 ttcttggata ggaagaatca aacacttttc cgttttctaa tgacccttcg tagtgaacag
    15721 atactgtttt tcctgcttca gcttgttttc catttcctct ttgaatgaat tggtaacgca
    15781 atcctgattc tgttttttcg aaacctgctg ctaatttttc cattttagct tcagattctg
    15841 ctttcaaagc agctaatttt ttcaatcgag ctcctttcaa accaacataa gcctcaatgg
    15901 cattccaatt tttcgcttcc gctcctactc taataatttc aagagtttct aatttatcgc
    15961 cttgagcaat agcatccact acatcttgac cttcgattac atgaccaaaa acagtgtgtt
    16021 tatcatctaa ccacggagtt gcaacgtgcg taataaaaaa ctgagaacca ttacttcctg
    16081 gccctgaatt tgccatagac aaaacccctg gcgcatcgtg acgcaaatct gaatgaaatt
    16141 catcatcaaa tttataccca ggatcaccag ttccagttcc aagaggacaa ccaccttgaa
    16201 tcataaaatt aggaatcact ctatgaaaat ttaaaccatc atagaatttt tgtccttgtg
    16261 gtttgatttt gttttccata tttccttctg caagtgctac aaaattccct acagttcctg
    16321 gtgttaaatc gtgtgttagt tttactaaaa tcgaaccttt tgcggtattg aatttagcgt
    16381 atattccgtt ttccattttt attgtttttt attgaggtgc aaatttacaa aaataattag
    16441 agttgacgat ttgcaaatac agtgattttt aaaattccct ttttaataag tcattttttg
    16501 cataaaatct tcgcgaattg gactaaaaac atctataagc aaaccatctt caatacaaac
    16561 cacaccgtga ataatatgcg gcggaatata gaaacaatcg ccttgtttca ttactttggt
    16621 cactccatta atagtcactt caaaagcacc tttagcaaca ttagttactt gcgaatgata
    16681 atgttcgtga ggcgttccga tagctccttt ttcgaaaaga acattgacca tcataatcgt
    16741 atcatcataa cccattattt ttcgtttgat gcctttgcca accgcttccc agtcgaattc
    16801 atcgtctttt ataaattctt tacttgctcc aaaactttgc atatgttatt gtttttgtgt
    16861 ctaaaattcg ttcttgtaaa agtaattttt ttcaattaag aaacaatgaa atttatcaca
    16921 ccaattccta gcccagatag cagtggaaat cctcccgatt tttcttcggg agattgtaac
    16981 gaatagctgg aaatagctcc tgaaaaaaaa ctttgtacat ttgcatccta gaaaagaaaa
    17041 aaaatgcgca tagatattat aaccgtatta cctgaattat tgcgaagtcc gtttgaggct
    17101 tcgataatga aacgtgctat tgacaaagga attgtggaag ttcattttca taatctgaga
    17161 gattatacga ccaataaaca aaaaagtgtg gacgattatc cgtatggtgg tggtgctgga
    17221 atggtaatga atatccagcc tattgacgat tgtattacgc acttgaaaag cgaaagaact
    17281 tacgacgaaa tcatttatat gtcgccagat ggtgatactt tgaaccaaaa aatggccaac
    17341 acaatgtcga tgtatgaaaa tattattatt ctttgtggtc attataaagg tgtggatcaa
    17401 cgcgttcgtg atcattttat caccaaagaa atttcgattg gcgattatgt tttgagtggt
    17461 ggagaattgg gtgctttggt gttatgcgat tctataattc gattgattcc aggtgttttg
    17521 agtgatgaaa cttcggcatt gacggatagt tttcaagata atttattgtc aggtccaata
    17581 tatacaagac ctgctgatta taaaggatgg aaagttcccg aagttttaac cagcggaaat
    17641 tttgccaaaa tagacaaatg gagagaagat atggcttatg aacatactaa aaatagacga
    17701 ccggatttgc tagaatagtc tgaaagtcca aagttcaaaa gtctgaaaat aacgttatgc
    17761 gaaaatatca ataaaaagat taaaaaaaat cgtaattcat aattcataat tcataattta
    17821 taattatttt tgcgccctca ttagaccaac ctctggcgaa atccgtgaat gttgctcttt
    17881 ttaaaaccat aaaataaaaa taaaatggca gatttattga aattcgttca agacgaattc
    17941 gtaacaagaa aagatttccc tgaatttgga gctggagaca caatcacagt tttctatgaa
    18001 attaaagagg gtgaaaaaac aagaacacag ttttttaaag gtgttgttat tcaaagaaga
    18061 ggttctggaa acacagaaac tttcacaatt cgtaaaatgt caggtgc
//
LOCUS       DAOSNN010000017        18033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3129_length_18033_cov_6.093225, whole genome shotgun sequence.
ACCESSION   DAOSNN010000017 DAOSNN010000000
VERSION     DAOSNN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 18033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18033
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3129_length_18033_cov_6.093225"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..617)
                     /gene="nadD"
                     /locus_tag="PLQ70_01840"
     CDS             complement(36..617)
                     /gene="nadD"
                     /locus_tag="PLQ70_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HQE33386.1"
                     /translation="MKIGLYFGTFNPIHIGHLIIANHFAEYSGLDQIWMVVTPHNPLK
                     NKQTLLDDYQRLQLVFLATEDYPKIKPSDIEFKLSQPNYTVNTLAHLQDKYPEHEFSL
                     IMGEDNLKSLHKWKNQEVILQNYEIYVYPRISSELENSVYKNNPKVHIIDAPIVEISS
                     TFIRENIKNKKNVRPLLPEKVWEYIDHNNLYKK"
     gene            complement(688..1257)
                     /gene="gmk"
                     /locus_tag="PLQ70_01845"
     CDS             complement(688..1257)
                     /gene="gmk"
                     /locus_tag="PLQ70_01845"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQE33387.1"
                     /translation="MKKGKLIVFSAPSGSGKTTIVRHLLKQEDLNLEFSISAATREAR
                     GEEVSGKDYYFMSLKDFKTHIKNEDFVEWEEVYRDNFYGTLKSEVERIWAQGKNVIFD
                     IDVAGGLRIKHKFPEETLAVFVKPPSVDELKRRLKERSTESEEKINMRIAKAHVELAT
                     APQFDVVIKNYDLEIALEEAHQLVKDFIK"
     gene            complement(1318..2178)
                     /locus_tag="PLQ70_01850"
     CDS             complement(1318..2178)
                     /locus_tag="PLQ70_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQE33388.1"
                     /translation="MIQSMTGFGKASLQLPTKKITVEVKSLNSKGLDLSVRMPSSYRE
                     MELGLRNQIALKLERGKVDFSIFIESTAEQTSTKVNVPIVKAYISQLREVYPDADETE
                     LMKMAVRMPDTLKTEREEIDENDWIEIQKVIAEALDYITNFRKDEGASLEKEFQLRIG
                     NIRQYMNDALALDPERVQAIKDRLQTAISELKVNVDENRFEQELIYYLEKLDITEEKV
                     RLTNHLDYFLETINGTEANGRKLGFITQEMGREINTMGSKSNHAQMQKLVVMMKDELE
                     KIKEQVLNVL"
     gene            complement(2279..3176)
                     /locus_tag="PLQ70_01855"
                     /pseudo
     CDS             complement(2279..3176)
                     /locus_tag="PLQ70_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(3169..3525)
                     /locus_tag="PLQ70_01860"
     CDS             complement(3169..3525)
                     /locus_tag="PLQ70_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HQE33389.1"
                     /translation="MNKIYYLASCDTCRKIIKSLPQNHNLTFHDIKQDPITIEELEKM
                     HQLSGSYEALFSKKAQLYKSMDLKNKSLTEADFKKYILEHYTFLSRPVFIIDSKIFIG
                     NSQQNILQVMKALSNV"
     gene            3622..4077
                     /locus_tag="PLQ70_01865"
     CDS             3622..4077
                     /locus_tag="PLQ70_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQE33390.1"
                     /translation="MTSAFNVNTTSRNMISKILEKHTLEQLNKIPEGFGNNLIWNIAH
                     VIVTQQLLVYKLSGLPIMVSDELVEKYKKGTKPEQAATQDEVDEIKSLLFSTIEKTKE
                     DYANGIFKNYTEYPTSTGFVLNSVQDALNFNNFHEGLHIGILMSIRKLV"
     gene            complement(4156..4959)
                     /locus_tag="PLQ70_01870"
     CDS             complement(4156..4959)
                     /locus_tag="PLQ70_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HQE33391.1"
                     /translation="MIQKFIAFFCLFLFGFSNVAIAQFGFSHEIGVIAGPVAFQSDYG
                     ERHDLSTNAGNSGFGIGLIHYMNFSYTAECNCYTPETYFNDHFKLRSELSYNKTKLNH
                     FGQWVEGKPSLGKEQLKAMSGSTAVTNVGMQLEFFPLSIRDFTETIGSFGPFISLGGQ
                     YSFYNTKALSSMGPLGTPLTTFPKYLTPTDDRSYGFSTESSSVWSVVSSVGTRYKLSP
                     LSDLMVDLRLQYYFSDWVDGLKPNPDLYKENKANDWLVWFNVGYIYYLQ"
     gene            5292..6635
                     /gene="gdhA"
                     /locus_tag="PLQ70_01875"
     CDS             5292..6635
                     /gene="gdhA"
                     /locus_tag="PLQ70_01875"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HQE33392.1"
                     /translation="MEEKINNFMAQVEAKNPNEPEFIQAVREFAETVIPFIASKRKYD
                     GKHILLRITEPERTITFRVVWVDDTGEIKVNRGFRIQMNSAIGPYKGGIRFHNSVNLS
                     VLKFLAFEQVFKNSLTTLPMGGGKGGSDFDPQGKSDGEIMRFCQSFMTELCRHIGPEI
                     DVPAGDIGVGAREIGYLFGQYKRIRNEFTGVLTGKGLAYGGSLIRPEATGYGVVYFTE
                     QMLHTIGQEIKGKTFVISGFGNVAWGVALKINELGGKLLTISGPDGYIYDADGISGEK
                     IDYMLEMRDSGTNRAEMYVEKYPNAVFHKGKRVWEVKADIAIPCATQNELNEEDAIKL
                     VENGVICVTEAANMPCTLDAIKYLLGKKILFAPGKAANAGGVAASGLEMTQNSIRLNW
                     SSEEVDNRLKEIMVGIHNQCKKYGTDETGYVDYVTGANIAGFVKVADAMLAQGIV"
     gene            6771..9065
                     /locus_tag="PLQ70_01880"
     CDS             6771..9065
                     /locus_tag="PLQ70_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33393.1"
                     /translation="MKNRFLYFVFLLFLQISFAQNNMVWQGYFSYNDIKDVSQSSTTL
                     FAASENALFSKNTSTGQLKTTNTIDGLSGQTITSLLHSSTANKTLVGYENGLMIVINE
                     ADGSMLNIVDIISKQLPANLKKINHFMEYNGIVYVSCDFGIVQFNLATMLFGDTYFIG
                     NNGSEISVKQTTVFNGFIYAATIDGIRKADSTNKNLVDFNQWSLIASGSWSSVEAFDT
                     QLIAINASGNVQKYDSATNFFNGLFQLSQAAVDMRATDNYLMITTPTTVSVYNKQMGV
                     VRQITSSQIVGTTVSFTCATNIDDTLYIGTNENGMYSTTISVSSAIENTMPSGPSRNN
                     IFAIQATTTSLWAVYGDYNLFYNPYPLDTYGISKFSETGWLSIPYDKVFGAKSISRIT
                     VNPSNEKEVYASSFFSGLLKIVDDVPKILYDQSNSGLESLILAPPNPSYIDIRINGAA
                     FDKNGILWVTNSLIKNGLKTLDTSGKWQSYSLDKILLASSNTNFARLSIDKNGTKWIA
                     TDRDGLIGFNEKNNVFKKITIGADTGNLPDANVRVAAADNNNQLWIGTTKGLRVLSNV
                     GSFSSENQLTTKPIIILEDGLAQELMYEQFITDIVVDGANNKWIGTVDSGVFLVSSNG
                     QETKYHFTETNSPLPSNTINDIDINSVTGEVFIATTKGMISFKGTATGANEDLNNVFV
                     YPNPVRPEYEGTVKISGLLNKATVKITDIEGNLVYETTSEGGTIEWDTTAFGKHKVAS
                     GVYMIFISAQDGIETKVKKVMIVR"
     gene            9069..10625
                     /locus_tag="PLQ70_01885"
     CDS             9069..10625
                     /locus_tag="PLQ70_01885"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQE33394.1"
                     /translation="MSNRVNQKALIIGGGPAGLTAAYELIKHSKVLPIVIEMSEFWGG
                     ISRTENHNGNRIDIGGHRFFSKSEIVMNWWQEILPICSDESSISISYQNDKKNIEIDG
                     KNISDKDDNILLVRKRKSRIFFNKKFFEYPISLSFQTILSLGLFNTFLIGISYTKAVL
                     FPIKKQTTLEHFFINRFGSRLYKTFFKDYTEKVWGVSCTEISSEWGAQRIKGLSVTKT
                     LTNAVTSIFRNNKKDIRQKSTETSLIEYFLYPKFGPGQMWETVAEKVKKEGAILKQNT
                     KVIAINIENNKVNSVVIENSLTKEIETIACDYCFSTMPIQELIKGFSCEVPMKVKEVA
                     KGLIYRDFVTVGLLLNNVNFQLEDNWIYIQESYVKVGRLQVFNNWSPFMVNDADKTWV
                     GLEYFCNQTDPIWHFSESEMVQLATDEMIQLGFVNSGENVLDAKVIKVPKTYPAYFGS
                     YSDFNEIRNFTDTIENLFLVGRNGMHKYNNQDHSMLTAMQAVQNIINNNTDKSNIWSI
                     NTEEEYHEQK"
     gene            11914..12627
                     /gene="recO"
                     /locus_tag="PLQ70_01890"
     CDS             11914..12627
                     /gene="recO"
                     /locus_tag="PLQ70_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQE33395.1"
                     /translation="MLVKTKAIVLSSLKYQEKSLIVKCFTQSHGLKTYFVRDAFSSRK
                     SNQKIAYFQPLTILEIEAVHKNKGTLENFKEIKIATPFQTIHSDIFKSTIVMFISEIL
                     HHSIHEEEKNEQLFTFLETALHWLDNHNQIANFHLILMLEITKYLGFYPDISEMDFPF
                     FDANEGVFTPFHGLGSLTEHETNLFKKLIALKFDNDQKIFHVIERQILLKTLIDYYTF
                     HLGGFRKPKSLEVLSAVFS"
     gene            12715..16116
                     /gene="ileS"
                     /locus_tag="PLQ70_01895"
     CDS             12715..16116
                     /gene="ileS"
                     /locus_tag="PLQ70_01895"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQE33396.1"
                     /translation="MSTKFTEYKGLNLPTAASEVLDFWKKENIFEKSVTTREGNTPFV
                     FFEGPPSANGLPGIHHVMARAIKDIFCRYKTQKGFQVKRKAGWDTHGLPVELGTEASL
                     GITKEDIGKTISVAEYNEACKKTVMRYTDVWNDLTEKMGYWVDMEDPYVTYKSKYMET
                     VWWILKQIYNKDLMYKGYTIQPYSPKAGTGLSSHEVNQPGSYRDVTDTTIVAQFKALN
                     ETLPSFLQGFGDIHILAWTTTPWTLPSNTALTVGPKIDYVLVETFNQYTFRPVKVILA
                     KNLVGKQFSKGFFPSTEAADFENFKEGDKKIPYQILTECKGADLVGIRYEQLMPLALP
                     YQNPENAFRVISGDFVTTEDGTGIVHTAPTFGADDAKVAKEATPEVPPMLVLDENGNP
                     VPLVNLQGKFIDGLGNYSGKYVKNEYYNEGEAPERSADVEIAIQLKEENKAFKVEKYV
                     HSYPHCWRTDTPILYYPLDSWFIKITEVRDRMFELNETINWKPKATGEGRFGNWLKNA
                     NDWNLSRSRFWGIPLPIWRNEEGTEEILIGSVEELYNEIEKSIEAGFQKENPFKGFEI
                     GNMDEANYDLIDLHKNVVDEITLVSASGKPMKRESDLIDVWFDSGSMPYAQWHYPFEN
                     KDKIDGNKDFPADFIAEGVDQTRGWFYTLHAIATLVFDKPSYKNVVSNGLVLDKNGHK
                     MSKRLGNATDPFETIAEYGPDATRWYMISNANPWDNLKFDIEGIAEVRRKFFGTLYNT
                     YSFFALYANIDNFTYAEADVPMNERPEIDRWIISELNTLVKVVDEAYADYEPTKAARA
                     ISEFVQENLSNWYVRLCRRRFWKGDYAQDKIAAYQTLYTCLLAISKLGAPIAPFYMDK
                     LYKDLTQATQSEKFDSVHLAEFPKFVENFVDKSLESKMQKAQTISSLVLSLRKKEMIK
                     VRQPLQKVMIPVLDENQRAEIEAVSDLIKAEVNVKEVTLLDDASGILVKQIKPNFKAL
                     GPRFGKDMGLIAKEIQSFSQVQISQLDKQGSIDIVISGNSITLSQEEVEISSQDIEGW
                     LVANSNGITVALDITISPELKEEGIARELVNRIQNIRKDSGFEVTDKIKVHLQKNAEL
                     ENAVMANEVYIKSETLTEVLVFEDTINNGIEIEFDDIKTKISITK"
     gene            16124..16504
                     /locus_tag="PLQ70_01900"
     CDS             16124..16504
                     /locus_tag="PLQ70_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HQE33397.1"
                     /translation="MIDEIARYSDADLAEFKELIIKKIQKAQADLDLIKSAYMNDLNN
                     GTDDTSPTFKAFEEGSETMSKEANSQLAIRQEKFIRDLKNALFRVENKTYGTCKVTGK
                     LISKERLLIVPHATMSIEAKNLQR"
     gene            16620..17228
                     /locus_tag="PLQ70_01905"
     CDS             16620..17228
                     /locus_tag="PLQ70_01905"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HQE33398.1"
                     /translation="MSLRKAYLIVFIILLVDQFSKIYIKTNFVYGESGQIDVTSWFKI
                     LLIENEGMAWGTVIPGIYGKLFLTVFRLVAVTGIGYWLWDSVERKHSSNYLIVAISLI
                     LAGAFGNIIDSVFYGVIFDDSYGHLATIFSEKPYGTWFHGKVVDMLYFPLFEGNWPEW
                     LPVLGGQHFKFFNAIFNVADAAISIGVGILLVFNKKAFHHQH"
     gene            complement(17319..17924)
                     /locus_tag="PLQ70_01910"
     CDS             complement(17319..17924)
                     /locus_tag="PLQ70_01910"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQE33399.1"
                     /translation="MAFELPQLPYAYDALEPHIDARTMEIHHTKHHNAYTTNLNAAIA
                     GTELEGKSIEEILGNLDLKNGAVRNNGGGFFNHNLFWTVMSPNGGGLPTGEVAAAIDA
                     AFGSFDAFKAAFSKAGATQFGSGWAWLCVKDGKLEVCGTPNQDNTLMPGIGCGGTPIL
                     GMDVWEHAYYLNYQNRRPDYIEAFFNVINWDEVAKRYAAAK"
BASE COUNT         6002 a         2995 c         3326 g         5710 t
ORIGIN      
        1 ttactattta ttaattgaat agattttctt caattctact ttttgtatag attattgtgg
       61 tcgatgtatt cccaaacttt ttcaggtaat agtggtcgaa cattcttctt gtttttgatg
      121 ttttcgcgaa taaatgtcga agagatttct acaatgggag catcaataat atgaaccttt
      181 ggattgtttt tataaaccga attttcaagt tccgatgaaa tgcgaggata gacataaatc
      241 tcgtaatttt gcaaaataac ttcttggttt ttccatttgt gcaatgactt caagttgtct
      301 tcgcccataa tcaacgaaaa ttcgtgttcc ggatatttgt cctgcaaatg cgccaaagta
      361 ttcacggtat aattcggttg cgacaatttg aattcgatat ccgaaggctt gatttttgga
      421 taatcttcgg tggccaaaaa aaccaattgc agtcgttggt aatcatccaa taaagtttgc
      481 ttgtttttca acggattgtg cggggttacg accatccaaa tttgatccaa acccgaatat
      541 tcagcaaagt ggttggcaat aatcaagtgc ccaatatgga tgggattgaa tgttccgaaa
      601 tagagaccga ttttcataat ttaggtttta ggttttaggt tttgggtttt ggattttact
      661 aatacccaat acctaaaacc taatgcctta ttttataaaa tccttcacca attgatgtgc
      721 ttcttctaaa gcaatttcca aatcatagtt tttaatcaca acatcaaatt gtggtgctgt
      781 cgccaattcg acgtgggctt tggcaatacg catattgatt ttttcttcac tttctgtaga
      841 acgttccttg agtcttcttt tgagttcgtc cacgcttggt ggtttcacga aaacagccaa
      901 ggtttcttca gggaatttat gcttgatgcg caaccctccg gcaacgtcaa tgtcgaaaat
      961 cacgtttttt ccttgtgccc aaatgcgttc gacttcgctt tttagcgttc cgtaaaaatt
     1021 atctctatag acttcttccc attccacaaa atcttcgttt ttgatgtggg ttttaaaatc
     1081 ttttaacgac ataaagtaat aatcttttcc gcttacttct tcgccacgag cttcacgcgt
     1141 tgccgccgaa atcgaaaact ccaaattcaa gtcttcttgc tttaataaat gcctaacgat
     1201 ggtggttttc cctgaacccg aaggtgctga gaatactatt aattttcctt ttttcattta
     1261 tattgttttt ggtagagacg cgattaatcg cgtctttacg gtatcaatta attgtgttta
     1321 caacacattc aaaacctgtt ccttaatttt ctccaattca tccttcatca tcacgaccag
     1381 tttttgcatt tgggcgtgat tcgatttaga acccattgtg ttgatttcac gacccatttc
     1441 ttgggtgatg aaacctagtt ttctaccatt agcttcggtt ccgttgatgg tttctagaaa
     1501 ataatccaag tgattggtca aacgtacttt ttcttcggtg atgtccagtt tttcaagata
     1561 ataaatcaat tcttgttcga aacggttttc gtccacattg acttttaatt cagaaatagc
     1621 agtttgcaaa cggtctttta tcgcctgaac tcgttctgga tcaagagcca aggcatcgtt
     1681 catatattga cggatgtttc caatgcgtaa ctggaattct ttctccaaag aagcaccttc
     1741 gtcttttcgg aaattggtga tataatccaa agcttccgca attacttttt gaatttcaat
     1801 ccagtcgttt tcgtcaattt cttctctttc ggtttttaat gtatcgggca ttcttacggc
     1861 cattttcatc aattcagttt catcggcatc aggatatact tcccgcaatt gagaaatgta
     1921 ggctttcacg atgggcacgt tcactttcgt ggaagtttgc tcggcggtac tttcgataaa
     1981 aatcgagaaa tccacttttc ctctttcgag tttcaaggcg atttggttgc gtaaacccag
     2041 ttccatttcg cggtaagacg aaggcattct cacgcttaaa tctaagcctt tgctgttcag
     2101 ggattttact tctactgtta tttttttggt tggcaattgc aagcttgctt taccaaatcc
     2161 agtcattgat tgtatcatat atttttctaa aagagttcaa agataagaaa agttcagagt
     2221 aattagtaat tagtgattag ttgctacaaa aagctattta ctaattacta atggctaatc
     2281 actttttttg cgttaccaaa tacacacctg caaatattaa aatagccgaa acgattttga
     2341 ccaaagtcag ttcgtctttt cctaaactaa tggcaaaaat ggtagcgaac aagggttgta
     2401 agtaaacaaa aacggcgatg gtggtgggtt ttaattctcg catcgaaagc aaattcaaca
     2461 gataagttcc gaaggtcgaa aacaccacca caaaacctat ttttaaataa atatccattg
     2521 gcatcgaagt ccaatttacg gcatcgaatt cgttccaacc aaaaggtaaa accattataa
     2581 aaccaattag ataaatccat ttggcaaagt tgaagggatt gtatttttgc atcaattttt
     2641 tgacaagaac caagtacaat ccgtaggaac ttgcattgat gaaaactaga aaattcccca
     2701 aggttggatt ggtggcattt cccgaagatt ttccgtaaag tatcagaaaa acagtgccga
     2761 tgagtcctaa aacaattcca aaaaccattc gtttttgcat tcgttccttg agaataatgg
     2821 ccgaaagtac gagtacaata ataggtgttg aaaccattag taccgaagcc gaaatgggcg
     2881 aagtcaaact caatcctttg aagaaagaca gcatattcag tgctacgcca aaaaaaagct
     2941 gcagcaataa ttcttgggta atcttttggg tctatttgct cttctcgaat tgctgaggat
     3001 ttcctgctaa aaaaccaagc caaccaaaac aaaatgctgg ctccacccac ccgcattatg
     3061 ataaacccgt atgcatcgac atattggggc atcacgtctt tggcaattgt gaaggtcatt
     3121 ccataaataa tggaaacgat ggtggcggta atgagggcaa gggttttttt agacattcga
     3181 taacgctttc attacttgaa gaatattttg ttgactgttg ccaatgaata ttttgctgtc
     3241 aataataaaa acggggcgac tcaaaaaagt gtaatgttcc agaatgtatt ttttgaaatc
     3301 ggcttcggtc aaggatttgt ttttcaaatc catcgatttg tagagttgtg cttttttgct
     3361 aaaaagtgct tcgtaacttc ccgaaagttg gtgcattttc tccaattctt caatcgtaat
     3421 ggggtcttgt ttgatgtcgt gaaaggtcaa attatgattt tgtggcaagg atttgatgat
     3481 ttttcgacaa gtatcgcagg aagcgaggta gtatattttg ttcataacgg taggtacaat
     3541 ttctttatgc aaagaaaatc catttacaac ggaaaatgat tatttttatc gaaaatttga
     3601 aaaacaataa aatcgaaaaa aatgacttca gcattcaacg tgaatacaac tagccgaaat
     3661 atgatttcta aaattttaga aaaacacaca ttggaacaac tcaataaaat tcctgaagga
     3721 tttggtaata atttgatttg gaatattgcc cacgttattg tgacacaaca acttttggtt
     3781 tataaacttt caggattgcc aataatggtt tcggatgaat tggtagaaaa atacaaaaaa
     3841 ggaaccaaac cagaacaagc agcaactcaa gacgaagtag atgaaatcaa gtctttgttg
     3901 ttctcaacca tcgaaaaaac aaaggaagat tacgccaatg gaattttcaa aaactataca
     3961 gagtatccaa cttcaacagg ttttgttttg aatagcgttc aagatgcatt gaatttcaat
     4021 aattttcacg aaggacttca catagggatt ttgatgagta ttcgaaagtt ggtttaaact
     4081 attttgaagt ataaaaaaat cccaattttg aaatagttga aaaattggga tttttttata
     4141 ttgtcttaat tttgactatt gtaagtaata aatgtaaccc acattgaacc aaaccagcca
     4201 gtcgttggct ttgttttctt tataaaggtc aggattaggt tttaatccat ctacccaatc
     4261 cgaaaaataa tattgcaatc ttaaatcgac catcaaatca gataatggag aaagtttgta
     4321 acgagttccc acacttgaaa ctaccgacca aactgaacta gattcagtag aaaaaccata
     4381 agagcggtca tcggttggcg ttaaatattt aggaaaagtg gtcaaaggag ttcccaatgg
     4441 tcccattgat gacaaagctt ttgtattata aaaactgtat tgtcctccaa ggctaataaa
     4501 aggaccaaaa cttccaatgg tttctgtaaa atcgcgaata cttaaaggga aaaattccaa
     4561 ttgcataccg acgttggtaa cagctgtact tccgctcatt gcttttaatt gttctttgcc
     4621 caatgagggt ttgccttcaa cccattgtcc gaaatgattt aattttgttt tgttatatga
     4681 caattcgctt cttaatttga aatggtcatt aaaataagtt tcaggagtat aacaattaca
     4741 ttctgctgtg taagagaaat tcatataatg aattaaacca attccaaatc ctgaattacc
     4801 agcatttgtt gataaatcgt gtctttctcc atagtcagat tgaaaggcta caggaccagc
     4861 aattactcca atttcgtgtg aaaatccaaa ttgtgcaatc gctacatttg aaaatccgaa
     4921 gaggaaaaga cagaaaaatg caatgaattt ttgaatcatt ctaaatgaat tactttttta
     4981 caaatatata aaaacatcaa tcaatcctta tgttttctat gtaaatatga taaaacgata
     5041 aacaatagaa aaattatata aataaactac acaaattttt gtgttttgtc atcaatagtt
     5101 ttggttttga ataaaatcat tccaacatac ataaaaagca ttcgcaatgt gttaagaatc
     5161 attaaatttg gatacaaata ttctcatata ctgtatattt gcaagactta cgaaaacgtt
     5221 ttcttaaaaa acagaaagtt ttggttaagt aaacgattaa tagtaaattt gatacactaa
     5281 tttatttcaa tatggaagaa aaaatcaata attttatggc tcaagttgag gccaaaaatc
     5341 caaatgagcc tgaatttatt caagccgtta gagaatttgc tgaaacagta attcctttta
     5401 tagcttcgaa acgaaaatat gatggtaaac atattcttct tagaattacc gaacccgaaa
     5461 gaacaataac ttttcgagta gtttgggttg atgatacagg agaaattaaa gtcaatagag
     5521 ggtttagaat tcagatgaat tcagctattg gaccttataa aggaggaatt cgttttcaca
     5581 attcagttaa tttatcggtg cttaaatttc ttgctttcga acaagtattc aaaaatagtt
     5641 taacaacact tccaatggga ggcggaaaag gaggttcgga ttttgatcct caaggcaaat
     5701 ctgatggcga aataatgcgt ttttgtcaat cttttatgac cgaattgtgt cgtcatattg
     5761 gaccagaaat tgatgttcct gctggagata ttggtgtagg agcaagagaa attggctatt
     5821 tgtttggtca gtacaaacgc attagaaatg aatttacagg agttttaact ggaaaagggc
     5881 tcgcttatgg cggttcactg attcgaccag aagcaacagg atatggcgtg gtttatttta
     5941 cagaacaaat gcttcatacc atcgggcaag aaataaaagg aaaaactttt gttatttctg
     6001 gatttggtaa tgtggcttgg ggagtagctt tgaaaataaa tgaattagga ggaaaacttt
     6061 tgaccatttc gggaccagat ggctacatat atgatgcaga cggaatttcg ggcgaaaaaa
     6121 ttgattatat gcttgagatg agagatagcg gaactaatag agccgaaatg tatgtagaaa
     6181 aatatccaaa tgccgttttt cacaaaggaa aacgagtttg ggaagtaaaa gccgatatcg
     6241 ccattccgtg tgctactcaa aacgaattga atgaagaaga cgccataaaa ctagttgaaa
     6301 atggagtaat ttgtgttacc gaagccgcta atatgccttg tacattagat gctatcaaat
     6361 atttgttagg taaaaaaatt ctttttgctc ccgggaaagc cgctaatgct ggaggagtgg
     6421 cagcttctgg tttagagatg actcaaaact cgattcgttt gaactggtca agcgaagaag
     6481 tagataatag actaaaagag attatggttg gaattcacaa tcaatgtaaa aaatatggaa
     6541 ccgatgaaac tggatatgta gattatgtaa caggagccaa tattgcagga tttgttaaag
     6601 ttgccgatgc tatgcttgct cagggtatcg tttagatttt aaatttgact tattaatagc
     6661 ctttcttcaa cttgaaaatt caagatttga tgaaaggctt ttttttgtgg gtaacaaaat
     6721 cctaatactt tcgtttgtat tatatttgtg aacgaaatat tcttcaaata atgaaaaaca
     6781 gatttctata ttttgtgttt ttgctttttt tgcaaattag ttttgcccaa aacaacatgg
     6841 tgtggcaagg ctatttttct tataatgaca ttaaggatgt gtctcaatct tcgaccaccc
     6901 tttttgctgc ttcagaaaac gccttgtttt caaaaaacac aagtacaggt caattaaaaa
     6961 caaccaatac tattgatgga ctttcggggc aaacgataac ttctttattg catagttcta
     7021 ctgccaataa aactcttgtg ggatatgaaa atgggcttat gatagtaatc aatgaagccg
     7081 atggaagtat gctaaacatt gtggatatta tcagtaaaca acttccagca aacttaaaaa
     7141 agattaacca ttttatggaa tacaatggga ttgtttatgt ttcctgcgat tttggaatcg
     7201 tacaattcaa tttagcaact atgctttttg gtgataccta ttttattggt aataatggct
     7261 ccgaaataag tgtaaaacaa accacagttt ttaatggatt tatatatgct gccacaattg
     7321 atggcattag aaaagccgat agtacgaata aaaacttagt agatttcaat cagtggagtt
     7381 taattgcatc aggaagttgg tctagtgttg aagctttcga cacccaactt atagctatta
     7441 atgcttctgg aaatgttcag aaatacgatt cggcaacaaa tttctttaac ggattatttc
     7501 aactttctca agccgctgtc gatatgaggg ctacggataa ttatttgatg ataactacac
     7561 caacgactgt ttctgtttat aataagcaaa tgggagttgt tcgtcaaatt actagcagtc
     7621 aaattgtagg aacaactgta agttttactt gtgcaacaaa tattgacgac acactatata
     7681 ttggaaccaa tgaaaacgga atgtacagta caacaatttc agtgtcttcg gctatagaaa
     7741 atacaatgcc ctcagggcca agtcgtaata atatttttgc aatccaagca acaacaactt
     7801 cactttgggc tgtttatgga gattataatc ttttttataa tccttatcca ttggatactt
     7861 atggaattag caagtttagc gaaacaggct ggttaagtat tccttatgat aaagtatttg
     7921 gagcaaaatc catatccaga attaccgtta atcctagtaa tgaaaaagaa gtatatgcca
     7981 gttctttttt ttcgggatta ctcaaaattg tagatgatgt tccaaaaatt ttatatgatc
     8041 aatccaatag cggattagag tcgctaatat tagcaccgcc caatccgagt tatattgata
     8101 ttagaataaa tggagctgca tttgataaaa acggaattct ttgggttact aatagtttaa
     8161 taaaaaatgg attaaaaaca ttagacacaa gcggaaaatg gcaaagttat tctttagata
     8221 aaattttact agccagttct aatactaatt ttgctagatt gtctatcgat aaaaatggca
     8281 caaaatggat tgcaacagat agagatggtc taataggatt taatgaaaaa aataatgttt
     8341 tcaaaaaaat cactattgga gctgatacag gaaatttgcc ggatgccaat gtaagagttg
     8401 ctgcagcaga caataataat cagctctgga ttggaaccac aaaaggattg agggtgttgt
     8461 caaacgttgg aagtttttca tcggaaaatc aattaacgac aaaacccatc attattctcg
     8521 aagatggtct tgctcaagaa ttgatgtatg aacaatttat tacagatatc gttgttgatg
     8581 gtgctaataa taaatggatt ggaactgttg attcgggtgt gtttttagtt tcttcaaatg
     8641 gtcaggaaac aaagtatcat tttacagaaa ccaattcgcc attgcccagt aataccatta
     8701 atgatattga tattaatagt gtaactgggg aagtttttat agccacaacc aagggaatga
     8761 tatcttttaa aggaactgct acaggtgcaa acgaagattt aaataatgtg ttcgtatatc
     8821 caaatcctgt tcgcccagaa tatgagggaa cggtaaaaat atcaggatta ttaaacaaag
     8881 ccaccgtaaa aattaccgat atcgaaggga atttggttta tgaaactact tccgaagggg
     8941 gaacaataga atgggatact actgcttttg gaaaacacaa agtagcttca ggagtgtata
     9001 tgatttttat ttcggcacaa gatgggattg aaacgaaagt caaaaaagta atgattgtaa
     9061 gatgataaat gtcaaataga gtaaaccaaa aagcgctaat aatcgggggt ggtccagcag
     9121 gattaaccgc tgcatacgaa cttataaaac attccaaggt tttgccaata gttattgaaa
     9181 tgagtgaatt ttggggagga atttctagaa ctgaaaatca taatggcaac agaattgaca
     9241 ttggaggaca tcggtttttt tcaaaatcag aaattgttat gaactggtgg caagaaattc
     9301 tacctatttg ttcagatgaa agttcaatta gcatttcata tcaaaatgat aaaaaaaata
     9361 ttgaaattga tggcaaaaac atttccgaca aggatgacaa tattttatta gttagaaaac
     9421 gaaaatctag aatttttttc aacaagaaat ttttcgaata tccaattagt ttgtcctttc
     9481 aaaccatttt aagtctgggc ttatttaata cttttttaat aggtataagt tatactaaag
     9541 cggtgttgtt tcccattaaa aaacaaacaa cactagagca tttttttatt aataggtttg
     9601 gaagccgctt gtacaaaacg tttttcaagg actataccga aaaagtttgg ggggtttctt
     9661 gtactgaaat tagttcagaa tggggcgcac aacgaataaa aggtttgtct gttactaaaa
     9721 ctctaaccaa tgcagttaca agtattttta gaaataacaa aaaagatatt aggcaaaaaa
     9781 gcactgaaac ttctttgatt gaatattttt tgtatccaaa atttggtcca ggtcaaatgt
     9841 gggaaaccgt tgctgaaaaa gttaaaaaag agggtgcgat tttaaagcaa aatacaaagg
     9901 taattgcaat aaatattgaa aacaacaagg ttaatagcgt tgttatagaa aatagtttaa
     9961 caaaagaaat agaaacaata gcttgtgatt actgcttttc tactatgcct atacaagaat
    10021 taataaaagg gtttagttgt gaagttccga tgaaagtaaa agaagtggca aaaggcttaa
    10081 tctatagaga ttttgtcact gttggtttgc ttttaaacaa tgtcaatttt caattagagg
    10141 ataattggat ttatattcag gaatcatatg taaaagtggg acgacttcag gtatttaata
    10201 attggagtcc atttatggtt aatgatgctg ataaaacctg ggttggttta gaatattttt
    10261 gcaaccaaac cgatcctatt tggcactttt ctgaaagcga aatggtacaa ttagctactg
    10321 acgaaatgat acaattgggg tttgtaaatt cgggtgaaaa tgtattagat gccaaagtga
    10381 ttaaagttcc aaaaacctat ccggcctatt ttgggagtta ttcagatttt aatgaaataa
    10441 gaaactttac agatactatt gaaaatcttt tcttggtagg aagaaatgga atgcataaat
    10501 ataacaatca agaccattcg atgcttactg caatgcaagc cgttcaaaat ataatcaaca
    10561 acaataccga taaatctaac atttggtcta ttaataccga agaagaatat cacgaacaaa
    10621 aataagaaca atgctatttg aaatgaaaga ttttactaaa tcagaaaaaa aaatatttct
    10681 aataattgcc gttatgtatt ttgttttttt gatggcaaga atggtttcta ctatttattt
    10741 tttgacagat tccgatgaat attttgaagt agcaaaaaca attagaaatt tcacttactt
    10801 taatcattca aacaatccag aattaatcac aagaagacct tttgtttatc cttttttctt
    10861 gtctcttttc gttaatttta agcccttttt tatagttgtt cttcaaacag ttttcggtct
    10921 ttttaatgtc tttgtactgt ttaaaatatt gaagcattat aaggttcaaa tagctaataa
    10981 tctattagtt ctatttttgt ttacaccctc aatttttatc tatactcatg ttctaatgtc
    11041 agagtggttg gtgatgttgt ttcttacatt gcttttttgg ttgcttttgc aaaaatggac
    11101 caaaaacaac tttgcttata tccaaataat tactgtttta ttagccttta caaagcccat
    11161 tttttaccct tttatttaca tcaattctat attcttctta gtgtatatgt tgaaaaataa
    11221 agtttttagt ttttggttat ttttgccaat aatttgtttg caattctatt taaatcacaa
    11281 tgaaaatcta acaggttaca gacatttttc gtctatagaa aataaaaatt taatagacta
    11341 taatctatat tattttaaat cttcaaccca gtccaaagaa aaagctgatt tgtggttgga
    11401 ttcagtgtat aaagataata gatacgttgg aaagaatttt gcagaacaga atatttacct
    11461 caagcaaatg gcaaccaatg aaataaagaa aaacttgttt cgatattcct tttaccattt
    11521 ctatacggct gtcagaggca tatttgaccc aggaagatat gatttaatta ccttttttga
    11581 aaaggaaaat ggcagacaag gatttttgga agtcttaaat agcgacaaat ctatttggtc
    11641 actatttaaa aataaagtag catttgttta tttactatta attccaattt ttttgataaa
    11701 ttgtatgaaa tggttttatg ttattaagtg tctaatattt aataaattga ccttaaatat
    11761 gtattattta attgtattat ttgcttatta tatattggtt tcgggtcccg tgaattgttc
    11821 ccgatatatg atgccttttc aaggaatagt gatcgttttt gccgttttgg gaatgcaata
    11881 taaatcgaag tctctaaatc aaaaagcttc aaagtgttag tcaaaaccaa agccatcgtt
    11941 ctttcttctc tcaaatacca agagaaaagc ttgattgtga aatgctttac gcaatcacac
    12001 ggtttaaaaa cctattttgt tcgcgacgct ttttcttccc gaaaatcaaa ccagaaaatt
    12061 gcctatttcc aaccgctgac cattctcgaa attgaagccg tccataaaaa taagggaaca
    12121 ttggaaaact tcaaggaaat caagattgcc actccatttc aaaccattca ttcggatatt
    12181 ttcaagagca ccatcgtgat gttcatttcc gaaattctgc accattccat tcacgaagaa
    12241 gagaaaaacg aacaactttt tactttcttg gaaaccgctt tgcattggct cgacaatcac
    12301 aaccaaattg ccaattttca cctgattcta atgctcgaaa taaccaaata tctaggtttt
    12361 tatcctgata tttccgaaat ggattttcct tttttcgatg ccaacgaagg tgtttttacg
    12421 ccatttcacg gtctgggttc actcacggaa cacgaaacca atcttttcaa aaaactcatt
    12481 gctttgaaat tcgacaacga ccagaaaatc ttccacgtta tcgaaagaca aatcctttta
    12541 aaaacactga tcgattatta cacttttcat ctcggcggtt tccgaaaacc aaaatctttg
    12601 gaggttttgt cggcagtttt ttcttgaaaa attcaaccac aaaaactcat aactcataac
    12661 tcataattca taactatttc ttactttcgc accgcgatta aagaaaagga taaaatgagc
    12721 acaaaattta ctgaatacaa aggacttaac ctgcctacag cggcatcgga agtacttgat
    12781 ttttggaaaa aagaaaacat attcgagaag agtgtaacca ctcgcgaagg caacacccca
    12841 ttcgtgtttt ttgaaggacc accttcggcc aatggactgc cgggaattca ccacgtgatg
    12901 gcacgtgcga ttaaagatat tttttgccgt tataaaactc aaaaaggatt ccaagtgaag
    12961 cgtaaagcgg gctgggatac ccacggtttg cctgtagaat taggtaccga agcttctttg
    13021 ggaattacta aggaagatat tgggaaaacc atttccgtag ccgaatacaa cgaagcctgt
    13081 aaaaaaacag taatgcgtta cactgatgtg tggaacgatt tgaccgaaaa aatgggttat
    13141 tgggtcgata tggaagatcc gtatgtgact tacaaatcca agtatatgga aactgtttgg
    13201 tggattttga aacaaatcta caacaaggat ttgatgtaca agggttatac tattcagccg
    13261 tattctccaa aagctggaac aggattgtct tcgcacgaag tgaaccagcc gggaagttac
    13321 cgtgatgtaa cggatacgac gatagttgcc caattcaaag cattaaacga aactttacct
    13381 tcatttctgc aaggatttgg agatattcac atcttggctt ggacgacaac gccttggact
    13441 ttgccatcaa acacggcttt gaccgttggt ccaaaaattg attatgtgtt ggttgaaact
    13501 ttcaatcaat atactttccg acctgtcaaa gtaattctgg ctaaaaacct ggttggaaaa
    13561 caattttcaa aaggattttt cccaagtacc gaagctgctg attttgaaaa tttcaaggaa
    13621 ggagacaaga aaatcccata ccaaatcctg acagaatgca aaggagccga tttagtagga
    13681 attcgttacg aacaattgat gccattggct ttaccatatc aaaatccaga aaacgctttc
    13741 cgagtaattt caggggattt tgtgactacc gaagacggaa caggaattgt gcacaccgct
    13801 ccaacatttg gtgctgatga tgctaaagta gccaaagaag ctactcccga agtgccacca
    13861 atgttggttt tagacgaaaa cggaaacccg gttccattgg taaatttgca aggaaaattc
    13921 attgacggtt taggaaatta ttcgggtaaa tacgttaaaa atgaatatta taacgaaggc
    13981 gaagcaccgg aacgttctgc cgatgtggaa attgccattc agttaaaaga agaaaacaag
    14041 gcgttcaaag tagagaaata cgtgcacagt tacccacatt gctggagaac tgacacgcca
    14101 attttatatt atccgctgga ttcttggttt atcaaaatca cggaagtgag agacagaatg
    14161 ttcgaattga acgaaaccat caactggaaa ccaaaagcaa ccggggaagg acgtttcggg
    14221 aattggttga aaaacgccaa cgactggaac ttgtcacgct ctcgtttttg gggaattcca
    14281 ttgccaattt ggagaaacga agaaggaaca gaagaaatat taatcggttc tgttgaagaa
    14341 ttatacaacg aaatagaaaa atctatcgaa gcaggtttcc aaaaagaaaa ccctttcaaa
    14401 ggttttgaaa ttggaaatat ggacgaagca aattatgatt tgattgactt gcacaaaaat
    14461 gtggttgatg aaatcacctt ggtttctgct tctggaaaac caatgaaacg cgaatccgat
    14521 ttgattgacg tttggtttga ctcgggttca atgccgtatg cacaatggca ttatcctttt
    14581 gaaaacaaag acaaaatcga cggtaacaaa gattttcctg ccgatttcat tgccgaagga
    14641 gtggatcaaa ctcgtggttg gttttatacc ctgcacgcca ttgcgacttt ggttttcgat
    14701 aagccttcgt ataaaaacgt agtttctaac ggattggttt tggacaaaaa cggacataaa
    14761 atgtccaaac gtttaggaaa cgctaccgat ccttttgaaa caattgcaga atacggccca
    14821 gatgccacac gctggtatat gatttcgaat gcgaatcctt gggacaattt gaaatttgac
    14881 attgaaggaa ttgccgaggt tcgtcgtaag ttttttggaa ctttatacaa cacttattct
    14941 ttctttgcgt tgtatgccaa tatcgacaat ttcacatatg ccgaagccga tgttccgatg
    15001 aacgaaagac cggaaatcga tagatggatt atttcggaat tgaatacttt ggtgaaagtg
    15061 gtggacgaag cttatgctga ttacgaacca acgaaagcgg ctcgtgctat ttcggaattt
    15121 gtacaggaaa acttgagtaa ctggtacgtt cgtttgtgtc gtcgtcgttt ttggaaaggt
    15181 gattatgcac aagataaaat tgcggcttac caaacgttat atacttgttt gttagccatt
    15241 agtaaattgg gtgcgccaat cgctccgttc tatatggaca aactttacaa agacttaaca
    15301 caggcaacac aatcagaaaa attcgactct gtacatttgg ccgaatttcc aaaattcgtt
    15361 gaaaactttg ttgataaatc gttggagagc aaaatgcaga aagcacagac gatttcttca
    15421 ctagttttat cgcttcgaaa aaaggaaatg attaaggtgc gtcaaccgtt gcaaaaggta
    15481 atgataccag tacttgacga gaatcagcgt gccgaaattg aagccgtttc cgacctgatt
    15541 aaagcggaag ttaacgtaaa agaagttaca cttttggacg atgcttcggg aattttggtc
    15601 aagcaaatta aaccgaattt caaggcgtta ggaccccgat ttggaaaaga tatgggattg
    15661 attgccaaag agatacagtc attttcgcaa gtgcaaatca gtcaattaga caagcaagga
    15721 agcatagata ttgttatttc gggaaatagt ataactttat cgcaagaaga agtggagatt
    15781 tcttcgcaag atattgaggg ttggttggtt gccaactcaa acggaataac cgttgcgctt
    15841 gacatcacaa tctctccaga attgaaagaa gaaggaattg ccagagaatt ggtcaacagg
    15901 attcagaaca tcagaaaaga ttctggtttt gaggttactg acaaaattaa agtacatttg
    15961 caaaaaaatg ccgaattaga aaacgccgta atggcaaatg aagtttatat aaaatcagaa
    16021 acattaacag aagtattagt ttttgaagac accataaaca acggaataga aatagaattc
    16081 gacgacataa aaaccaaaat atcaattaca aaataattag aagatgatag atgaaattgc
    16141 aagatactca gacgcagatt tagctgagtt taaagagctt attattaaaa aaatacaaaa
    16201 agcacaagca gatttagatt tgataaaaag tgcttatatg aacgatttaa ataacggaac
    16261 cgacgatact tcgccaacat tcaaagcatt tgaagaagga agcgaaacca tgtctaaaga
    16321 agcaaactcg cagttggcaa ttcgtcaaga gaagtttatc cgtgacttga aaaatgcact
    16381 tttccgtgta gaaaacaaaa cttatggaac ttgtaaagta acaggcaaat taatcagcaa
    16441 agaacgattg ttgattgttc ctcacgcaac aatgagcatc gaagcaaaaa atttgcaacg
    16501 ataagcaatt caaaaattca atatgaaaat tccaaattcc aacaatgatt tataaattat
    16561 tggaatttgg aatttaattt ttagaattta cctatttttg cgccattaaa atttacaaaa
    16621 tgtctttacg aaaagcttat ctgatagttt ttatcatcct actggttgac caattttcta
    16681 aaatatatat taaaaccaat tttgtctatg gcgaaagtgg tcaaattgac gttaccagtt
    16741 ggttcaaaat tctattgata gaaaatgaag gaatggcttg gggaaccgtt attccaggaa
    16801 tttacggaaa attattcctt accgtttttc gtttagttgc cgtaacggga attggctatt
    16861 ggctatggga ttctgtcgaa agaaaacaca gttccaacta tttgattgtg gccatttctt
    16921 taattcttgc tggagctttt ggcaacatca tcgactctgt tttttatggc gtgattttcg
    16981 acgatagcta cggtcattta gctactatat tttctgaaaa accatatgga acttggtttc
    17041 atggcaaagt ggtcgatatg ttgtactttc cattattcga aggaaattgg cccgaatggt
    17101 tgccagtttt gggcggtcaa cattttaaat tcttcaatgc cattttcaat gttgccgatg
    17161 ccgctatttc tatcggggtc ggaattttgt tggttttcaa caaaaaagct tttcatcacc
    17221 aacattagga ttaaagttat gaataaaaaa aatagacgaa taaacttttc gtctatccgt
    17281 ctattctcta tgttctttat tctattttct aaaagagact attttgcagc agcatatctt
    17341 ttagctactt catcccagtt aattacgttg aagaaagctt caatataatc aggtcttctg
    17401 ttttggtagt tcaagtaata agcgtgttcc caaacatcca ttcctaagat tggagttccg
    17461 ccacaaccaa ttcctggcat caaagtgttg tcttgatttg gagttccaca aacttctagt
    17521 tttccgtctt tcacgcacaa ccaagcccat cctgaaccaa attgtgtcgc tccagcttta
    17581 gaaaaagcag ctttaaaggc atcaaatgaa ccaaaagcag catcaatggc agcagcaact
    17641 tcaccagttg gcaatccgcc accgtttgga gacattactg tccaaaataa attgtggtta
    17701 aaaaaacctc caccgttgtt gcgaacagct ccgtttttca aatccaaatt tcctaaaatt
    17761 tcttcaatgc ttttaccttc caattcagtt cctgcaatag cagcattcaa gttggtcgtg
    17821 taagcattat gatgtttggt gtgatggatt tccatcgtgc gcgcatcaat atgaggttct
    17881 agtgcgtcat acgcataagg taattgtggt aattcaaaag ccataattat ttttgtttat
    17941 tggtttataa aatattttat tcaaaattaa acaattaact ttgaaaagga aattctattt
    18001 cgttatagtt ttattaaata aattgatagt ggt
//
LOCUS       DAOSNN010000018        18030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3130_length_18030_cov_5.016634, whole genome shotgun sequence.
ACCESSION   DAOSNN010000018 DAOSNN010000000
VERSION     DAOSNN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 18030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18030
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3130_length_18030_cov_5.016634"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLQ70_01915"
     CDS             complement(<1..378)
                     /locus_tag="PLQ70_01915"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467679.1"
                     /note="4-deoxy-L-threo-5-hexosulose-uronate
                     ketol-isomerase; catalyzes the interconversion of
                     4-deoxy-L-threo-5-hexosulose uronate to
                     3-deoxy-D-glycero-2,5-hexodiulosonate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HQE33400.1"
                     /translation="MTKYSSRYASSPQAVKNYDTKQLREEFLIDNLMEEGEITFTYSH
                     YDRYITGSAVPTTTPLQLETIDILKMPNFLDRREMGIINVGGNGSVVVEGTIYELGFK
                     DALYIGSGNSEVIFKSDDAKNPAK"
     gene            complement(664..2103)
                     /locus_tag="PLQ70_01920"
     CDS             complement(664..2103)
                     /locus_tag="PLQ70_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE33401.1"
                     /translation="MSNPKIGNYRWSICALLFFATTINYLDRQVLSLTWNDFIAPEFH
                     WTNNDYGNITALFSIFYAVSLLFAGKLVDLLDTKKGFLWAIGVWSIGACLHAFAGIAT
                     SGWITGNWFVGFEGAKEAIKTVNDTGLVINVSVTLFIFARFVLAVGEAGNFPAAIKTT
                     AEYFPKKDRAFATSIFNAGATVGALAAPISIPFIAEAFGWEMSFIIIGALGFVWMGFW
                     TFMYDKPENHTKVSAAELEYIQQDDVADSKLVGYVPETTTKVTFKDCFRYKQTWAFAF
                     GKFMTDGVWWFFLFWTPAYLSSVYEMDSTEAAFPLFILYAITLLSIIGGWLPTYFVDK
                     KGMNPYEGRMRAMLIFAFFPLLALIAQPLGHISYWLPVIIIGISGAAHQAWSANIFTT
                     VGDMFPKKAIATITGIGGLAGGLGSTLINKGSGVLFDYTKETDMAFMGFKGIEAGYFI
                     IFSICAVCYLIGWSVMKSLVPKYSPITDL"
     gene            complement(2450..3118)
                     /locus_tag="PLQ70_01925"
     CDS             complement(2450..3118)
                     /locus_tag="PLQ70_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQE33402.1"
                     /translation="MGFFTKIKNFIVELWGVSDTSLNEQILKDIDSTRFVVLDTETTG
                     FDYENDRILCIGAIVLQNYRIPIQESLEIYIQQEHYNKATAQIHGILRASVLDRPNEL
                     EALQQLLDFVADSIIVAHHTSFDITMINKALERNGLPRLKNKTFDTAILYKKTLLNSP
                     LLDRKDSYSLDDLADKFDISKKDRHTAMGDAYITAIAFLKILNKLKEKKGFNFNNYLN
                     IDKK"
     gene            complement(3118..5025)
                     /locus_tag="PLQ70_01930"
     CDS             complement(3118..5025)
                     /locus_tag="PLQ70_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HQE33403.1"
                     /translation="MKNTISQRVADFLKNFPPFSFLKQRDLELISEQITIIYKEKDSV
                     VFAENEETHNSFYVVHKGAVALRGNHENEILDMCDEGDIFGLRPLIANENYKMEARTY
                     EESILYAIPISVFKPYALENKAVGNFLIESFASNTLNPFSKSHRGKLYGEESLEADTK
                     LLDLQEVKYSKKIVSCIATTTAKEAAEIMRHKNVGAILVVEETFPIGIITDKDLRNKI
                     VTGEFQITTPVSEIMTSPVITYPTKMTVTQAQIAMMKSDISHLCLTIDGTSNSKAVGM
                     LSKHDVMVSIGNNPAVLLREIKRAKKFKKIKQIRASIIHLLQGYLDQNIPMTLITKII
                     SELNDACTKQVIAIVLKKTETSPPVKFSWLALGSQGRSEQLLLTDQDNALIYEDVPEE
                     LEAETKKYFLELSARINKGLFEIGYDYCPAEMMASNPNWCMSLSDYKTKIHHWITNTG
                     KNEVLLSFIFFDYSLSFGDTELVNNLSDFIFEDVKANPTFYIHLVSGALQSQSPTGFF
                     REFLLEQDGAHKDFFNIKNRALMPLADAARVLILSHSVKSISNTAERFEKLAELEPNN
                     SELYTACAYSYKALLKFRTKQGLLHNDSGQYIALNTLSKAEKIKLKSTFKTIKELQEI
                     IRVRFNPSNIL"
     gene            complement(5103..5876)
                     /locus_tag="PLQ70_01935"
     CDS             complement(5103..5876)
                     /locus_tag="PLQ70_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQE33404.1"
                     /translation="MEVFLNPDAWIALLTLTFLEIILGIDNIIFISIVTGKLPPEKRK
                     KATKVGMFLAMFMRIALLFGISLLIGMKKPWFYIDFSWFSAGVTGQSLILLLGGLFLI
                     YKSTTEIHEKVDEKGEEEKTIKKTASKSFQGVLLQIIMIDLVFSFDSILTAVGMTNGV
                     SGALYIMIAAVIISVLIMMQFAVPVGAFVNRNPSIQILGLSFLILIGFMLITESAHLS
                     NALIFGSHVTPVQKGYLYFAISFSLFVEFLNMKVDKKNK"
     gene            complement(5932..6306)
                     /locus_tag="PLQ70_01940"
     CDS             complement(5932..6306)
                     /locus_tag="PLQ70_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV"
                     /protein_id="HQE33405.1"
                     /translation="MKKIISLLTLLLLLSCYNVEHNCKDFKTGKFKFEYEVDGAKKVT
                     VFERKDSIEIETFEGKTDTSSIRWVSDCEYILQKIHPKNRAEKKAITMKILTTSKNSY
                     TFEFGIVGSDQKQRGTVEKMEN"
     gene            complement(6314..6889)
                     /locus_tag="PLQ70_01945"
     CDS             complement(6314..6889)
                     /locus_tag="PLQ70_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HQE33406.1"
                     /translation="MEATNSYLESVKKQFLYYKTLGEKAIDQLEPEQLFVSINDETNS
                     IATIVKHLSGNMLSRWTDFLTSDGEKEWRNRDAEFEDTIKTKEKLLVLWEKGWNCFFD
                     TLNALQTEQLSTIIYIRNEGHTVIEAINRQLAHYPYHIGQIVFYAKILKKSEWTSLSI
                     PKNKSNAYNSEKFSKEKSIRNFTDDELNKLN"
     gene            complement(6892..7464)
                     /locus_tag="PLQ70_01950"
     CDS             complement(6892..7464)
                     /locus_tag="PLQ70_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQE33407.1"
                     /translation="MENLIHTHKTFEKISFTDKKVNNREFEDCIFKNCDFSNSNFSNN
                     TFMDCEFIDCNLSMMQLSGTSLKTVHFTTCKLLGIQFNSCADFMFGVSFQDCLLDYSS
                     FANKKMPKTKFNTCSMKEVSFIGTNLTNSSFENCNLDNAIFNDTQLAGVDFRTAQNFK
                     IDPEFNPMRKAKFSTQGIVGLLDKYDIKIE"
     gene            complement(7464..7685)
                     /locus_tag="PLQ70_01955"
     CDS             complement(7464..7685)
                     /locus_tag="PLQ70_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6095 family protein"
                     /protein_id="HQE33408.1"
                     /translation="MATNKELLSKGIKYLAGALPLLFIGPSLIYNAFINKQNVWHYLV
                     LGIGILTCLGAVYLMILGLKTMMRSLFND"
     gene            complement(7688..8506)
                     /gene="murQ"
                     /locus_tag="PLQ70_01960"
     CDS             complement(7688..8506)
                     /gene="murQ"
                     /locus_tag="PLQ70_01960"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HQE33409.1"
                     /translation="MTFTKTTEQSSKYEHLEKMSIQELLSNINQEDKTVPFAVEKALP
                     QIETLVTEIVAKMKLGGRLFYIGAGTSGRLGVVDASECPPTFGVPFDMVNGIIAGGDK
                     AIRRAVENAEDNTTQAWLDLKAQNINEKDVVVGIAASGTTPYVIGGLQACNKNNILTG
                     SISCNAGSPMSQTAKFAIEVIVGPEFVTGSSRMKAGTAQKLVLNMISTATMIQLGKVK
                     GNKMVDMQLSNSKLVDRGVKMIMGEINVSYEEGAELLKTHGSVRKAVIFYKSKL"
     gene            complement(8697..10259)
                     /locus_tag="PLQ70_01965"
     CDS             complement(8697..10259)
                     /locus_tag="PLQ70_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQE33410.1"
                     /translation="MNFFNRLRVLFVLLIILIGPVSHSQNETFTMTQIGANNLLKVPW
                     DLQYGPDGYLWVTEKRVGVVRVNPVTAQRDELIQLSDLSSTATGQDGLLGMALHPDFL
                     TTSPYVYLSYTHLVSGQNKQKIVRYTYTINGNDGSLYSPVVLIDNLPSSNDHQSGRLI
                     FGPDQKLYYTIGDQAVKVCASNLAQFLPTQLQIDQKNWANYPGKTLRLNLDGSIPTDN
                     PVLNGVKSHIYSYGHRNAQGLVFGSNGILYSDEQGPSSDDEINIINSGKNYGWPFVAG
                     VKDNLMYDADGCLTTETSFTATNYQDPIMSLFLANTYKDSACTDSWMCRPNIAPSSLE
                     IYESDAIPAWKNSLLLTSLKKGRVYRVKLDATGTAVVGNAIQYFYTQNRYRDIVAAPD
                     GKSFYLITDDSGKTADASGMNTVSVMQNPAAILKFTLNESLSVASLEKEPAFKFWPNP
                     AKHTLFIEIKEPNGGNFKAELINSSGQVVKKIKELQLGINETNIANLPVGVYILKLYS
                     NDTTWQESIIHI"
     gene            complement(10450..10917)
                     /locus_tag="PLQ70_01970"
     CDS             complement(10450..10917)
                     /locus_tag="PLQ70_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQE33411.1"
                     /translation="MSFNHIFKAKLTWDSVSKETIATTKTYFKTHQITIEGKENLTVS
                     AAKAFKGDPSLYNPEDLLLSSLVSCHMMSYLYVCSQNGIDVLSYQDDAEATLEVSENG
                     SGRFIEVRLYPKVVIQQSEKIDEAINLHVKANELCFIANSCNFKIVHYPTVSS"
     gene            complement(11095..11730)
                     /locus_tag="PLQ70_01975"
     CDS             complement(11095..11730)
                     /locus_tag="PLQ70_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33412.1"
                     /translation="MKKIRGNKRRLNKIEQWRINSLEIDFNFLQKSNKDYIKFRIFPW
                     NPVAMSLNNFPKPKGKIRKKILESFFDIYESWNIQLKELGKPYYLKIWLFEPDIYNSQ
                     VVCAINEKIEYYENIFLEVEIAKKISKSSLDKISNRINNYDWEYRIEENPIFENEFLR
                     ENFISEAAYLEEQKWLKNQLNKPHRIMDQKRNGIAEKIYLFKSSDVWIGEK"
     gene            complement(11782..12450)
                     /locus_tag="PLQ70_01980"
     CDS             complement(11782..12450)
                     /locus_tag="PLQ70_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQE33413.1"
                     /translation="MNYLLPLFSVLLGYGIALFLKPKSKTKLKLLLAFSGSFLLSLTV
                     MHLLPEVYESHNTNIGLFIMAGILFQIILEFFSKGAEHGHVHGHETMSHIPWLLFVSL
                     CIHAFLEGFPVSHHHNLALGIAIHHLPIAIILTMFFINAQLDKKAIFFFMITFAVMTP
                     LGTILSDYLPILNNYYNEITAVVIGILFHISSTIIFESSEGHKFNIAKVSMIVIGIVL
                     AYFI"
     gene            complement(12534..13277)
                     /locus_tag="PLQ70_01985"
     CDS             complement(12534..13277)
                     /locus_tag="PLQ70_01985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQE33414.1"
                     /translation="MPNSLNPKTHNWFASWFDTPYYHILYKERNYREAQVFMDNLTHY
                     LNLPEKAKVLDLCCGKGRHAIYLNQLGFDVIGADLSENSIAEANKNQNDNLHFQVHDM
                     REKFEDKFDAIFNLFTSFGYFEKDEDNLTSLKAMQESLTEHGFAVIDFMNVNQVIANL
                     VPEEVKTVDGIEFHIKRYVNDGHIYKEIDFEDQGEKFHFTEKVKALTLKDFEAMMEEA
                     GIYLLDIFGDYKLKKFHKTESERLIMIFK"
     gene            13346..14515
                     /locus_tag="PLQ70_01990"
     CDS             13346..14515
                     /locus_tag="PLQ70_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HQE33415.1"
                     /translation="MENNYRMIAKTFFGFEEILAEELKKLGAQEVEQGVRMVSFKGDK
                     GFMYKANLSLRTALKILKPIYSFKAINEQALYKGISGVNWSKYLNANQTFVIDATVHS
                     TYFNHSEFVSQKCKDAIVDQFRERTGQRPSIEKIHPDLRINIHIDKDQVSVALDTSGN
                     SLHQRGYRLATNIAPINEVLAAGILLLSGWDGQTDFLDPMCGSGTFLAEAAMIACNIP
                     ANINRKEFAFEKWKDWDNDLFDQIMDSLLKKVREFHHTIKGYDKAPSAVMKAKDNIKN
                     ANLDEYITIEERNFFDTEKTLEGKLHIVFNPPYDERLDIHMEQFYKNIGDTLKKNYPG
                     TNAWFITGNLEALKFVGLKPSRKIKLFNASIEARLVKYEMYEGSKRTKFQITDGR"
     gene            14566..14802
                     /locus_tag="PLQ70_01995"
     CDS             14566..14802
                     /locus_tag="PLQ70_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33416.1"
                     /translation="MTKLQVRAFLFQLGCFAILFISFQYLIGKYTNLEGLWIQFTAFV
                     VGTILGPKFQAAKTKEGEKLFMSWLFIKGIKDIT"
     gene            complement(14795..15631)
                     /locus_tag="PLQ70_02000"
     CDS             complement(14795..15631)
                     /locus_tag="PLQ70_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HQE33417.1"
                     /translation="MKHMLKYFFSIGLLMTVFCIQAQETTPTTKSLDKTKTEGIIPAS
                     PKQAQAKPAIATKTDTVVPVKKDRYGVRVGLDLYKLTRGLYDSNYKGIELVGDYRFTK
                     NYYLAAEIGSEDKTTEDDRLNTTTKGTYIKFGFDYNAYENWLNMENIISIGLRGGFST
                     FNQELNSYKIYNTQHYWDEMPWIASGESFNGLTAGWIEVVAGVKVKVYNNIFVGFSLR
                     MNTLLFEKKPDENFENLYIPGFNRTYAGNFGAGFNYTVTYFIPLYKKTVKPKEVVKKE
                     IK"
     gene            complement(15603..16142)
                     /locus_tag="PLQ70_02005"
     CDS             complement(15603..16142)
                     /locus_tag="PLQ70_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HQE33418.1"
                     /translation="MKKILSILLLVIVTSSSCEKDDICDANTPTTPRLVIDFYNITTP
                     SVKKNVTNLKVVGDGMKEGIVFNSSATGDDQYLTNGTSISIPLKTDTNSTKFSFTLNS
                     GNANPTLIDIDEVTFNYTRENVFVSRACGFKTLFTFDPTNPYIHSAVPSTKTKWMRVI
                     SIEKSNIANENETHVKIFF"
     gene            complement(16219..17634)
                     /gene="rlmD"
                     /locus_tag="PLQ70_02010"
     CDS             complement(16219..17634)
                     /gene="rlmD"
                     /locus_tag="PLQ70_02010"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HQE33419.1"
                     /translation="MAKKNTDKIVFDHIKVLDAGAKGVSVAKAPDGKVIFIPNVVPGD
                     VVDVQTFKKRKAYYEGKAVKFHEYSEHRIEPICEHFGVCGGCKWQNMKYSQQLYYKQN
                     EVQNHLQRIGKIELPDFEPILGSEKQFFYRNKMEFSFSNSRWLTEKEIGSTEDLGNRN
                     ALGFHIPKMWDKILDINKCHLQEDPSNQIRNEIRAFANANNLTFFNPRNHEGLLRTLM
                     MRTASTGEIMVLIQFFENDKAGRELLLDHLYEKFPQITSLQYVINNKANDTLYDQNIK
                     LYKGRDYILEEMEGLKFSINAKSFYQTNSDQAFELYKITRDFAGLTGTETVYDLYTGT
                     GTIAQFVSKKAKKVIGVESVPEAILDAKANAERNNITNCEFYVGDMKVVFNESFIAQH
                     GKPDVIITDPPRDGMHKDVIEQILKIEPEKVVYVSCNSATQARDLALMDEKYKVTRVR
                     PVDMFPQTHHVENVVLLEKRK"
BASE COUNT         5708 a         3065 c         2956 g         6301 t
ORIGIN      
        1 tttagctgga ttttttgcat catcactttt aaagataact tctgaatttc cacttccaat
       61 gtaaagagca tctttgaaac ctaattcata aatagttcct tcaacaacaa cagaaccatt
      121 tccacccaca ttgatgatac ccatttctct tctatctaaa aagtttggca ttttcaaaat
      181 gtcgatagtt tcaagctgaa gtggagtagt ggttggtaca gccgaaccag taatatatct
      241 atcgtaatgc gaataagtaa aagtaatttc tccttcttcc attagattat caatcaagaa
      301 ttcttctctt aattgtttcg tatcataatt ttttactgct tgagggctag aagcgtatct
      361 agagctatat tttgtcataa taatataatt tgtgtaatcg attgcgcaaa attaatttta
      421 atttaataat aaacaatatt ttaaattgtt tattttaggg atttatgttt tttatattga
      481 atattaataa atacggctga taaagtgaca gatgaattta taaatagact tatttgaatt
      541 tgcataataa agcattcttc atctaactga aaacataagt ttaactaagt ttaagaacgt
      601 gattaaaaga ttgtaaaaga ataatgcttc aaaaaatatt ttttgaagca ttataaatta
      661 ctattacaaa tctgttatag ggctgtattt aggaactaag gatttcataa ctgaccatcc
      721 gattaagtag caaacggcac aaatagaaaa tatgataaaa tatccggctt ctattccttt
      781 aaatcccata aaagccatat cagtttcttt agtataatca aataaaactc ctgaaccttt
      841 gttaattaat gtagaaccta atcctccagc taaaccacca attcctgtta tggttgcaat
      901 ggctttttta ggaaacatat cgccaactgt ggtaaagatg ttggccgacc aagcttggtg
      961 agcagcacct gaaataccaa ttatgattac tggcaaccaa tagctaatgt gtcctaatgg
     1021 ttgtgcaatt aatgctaata aagggaaaaa tgcaaaaatt aacatcgctc tcattctacc
     1081 ttcataagga ttcattcctt ttttatctac aaaataagtt ggtaaccaac ctccaataat
     1141 agaaagtaaa gtaattgcat aaagaataaa caaaggaaag gcagcttcag tagaatccat
     1201 ttcataaaca gaacttaaat aagctggtgt ccagaataaa aagaaccacc aaacaccgtc
     1261 ggtcatgaat tttccaaagg caaaagccca agtttgtttg tatctaaagc aatctttgaa
     1321 cgttactttt gtagttgttt ctggaacata acctactaat ttactatcag caacatcatc
     1381 ttgttggata tattctaatt cagcagcact aacttttgta tgattttctg gtttgtcata
     1441 cataaacgtc caaaatccca tccaaacaaa acctaaagct ccaataataa taaatgacat
     1501 ttcccatccg aaggcttcgg cgataaatgg aatagaaatt ggcgctgcta atgctccaac
     1561 cgttgctcct gcattgaaaa tactagtagc aaaagctcta tcttttttcg ggaaatattc
     1621 tgctgtagtt ttaattgctg cagggaagtt tcctgcttct ccaacggcta aaacaaaacg
     1681 tgcaaaaata aacaaggtaa cactaacgtt aattaccaaa ccagtatcat ttacagtttt
     1741 aatagcttct tttgctcctt caaaaccaac aaaccagttt ccggttatcc atcctgaggt
     1801 agcaattcct gcaaaagcgt gcaaacaggc accaatagac catactccaa tagcccaaag
     1861 aaatcctttt ttggtgtcta ataaatcgac taattttcca gcaaacaata aagaaactgc
     1921 atagaaaata gagaacaatg ctgtaatatt tccataatca ttgttcgtcc agtggaattc
     1981 aggagcgata aaatcgttcc aagttaaaga tagtacttgt ctgtctaggt agttgatggt
     2041 tgtcgcaaaa aaaagtaaag cgcaaatgct ccatctataa ttgcctattt ttgggttgct
     2101 cataatggtt attttaattt attaggtttt tttatttatt ttttaaaagc acatgtaatc
     2161 gattacacaa aaataagtct tattttttaa attcctattt ttttgctggt ttttttcata
     2221 tctaaattat taaattgtaa attattgctt tatactcaaa tatctcccat tttattggga
     2281 ttacgagaat ataaattaat actcaattgg tttataaatg tatttttaac acttttcatc
     2341 ttcgaaaata aattattttt caaatttgta tctctaattt tgtaattatt ggacggaatt
     2401 tttgaaaaat gaaagccaaa tatagagttt ttttcaattt ttagtagatt tattttttat
     2461 caatatttaa ataattattg aagttaaatc cttttttttc tttcaattta ttcaagattt
     2521 tcaaaaaagc aattgcagtt atataggcat cacccattgc cgtatgtctg tccttttttg
     2581 aaatgtcaaa tttatcggca agatcatcta aactatagct gtcttttcga tctaggagtg
     2641 gagaatttaa taatgttttt ttataaagaa ttgcagtgtc aaaggttttg ttttttaatc
     2701 ttggcaaacc atttctttct aaagctttgt ttatcattgt gatgtcaaaa ctagtatgat
     2761 gagccacgat tatggagtcg gcaacaaaat ccaataactg ttgtagagcc tctaattcgt
     2821 tggggcgatc aagtactgat gctctcaaaa tcccgtgaat ttgtgctgtg gctttattat
     2881 aatgttcttg ctgaatgtat atttctaaac tttcctgaat tggaatcctg taattttgca
     2941 aaacaattgc gccaatacac aatattctgt cattttcata atcgaaacca gtcgtttctg
     3001 tatccaaaac aacaaatcgt gtcgagtcga tatcttttag aatttgctca tttaatgatg
     3061 tgtcagaaac gccccaaagt tctactataa agtttttaat tttagtgaag aatcccatta
     3121 taatatgttt gaaggattaa atcgaactcg aatgatttct tgcaattcct tgatggtttt
     3181 aaaagtactt tttaacttta ttttttctgc tttcgataat gtatttaagg ctatatattg
     3241 gcctgaatca ttatgcaaaa ggccttgctt ggttctaaat ttgagtaatg ctttatacga
     3301 gtaagcacaa gcggtatata actcgctatt attgggttct aattcggcca atttttcaaa
     3361 ccgttctgca gtattgctaa tagatttgac cgaatgagat aggattaaaa ctcttgctgc
     3421 atcggcaagt ggcattaacg ctctgttttt tatattgaaa aaatctttgt gagctccgtc
     3481 ttgctctaat aaaaattctc taaaaaaacc tgtaggagat tgactttgta gcgcaccact
     3541 taccaaatgt atataaaagg taggattggc tttgacatct tcaaagataa aatccgacaa
     3601 attattaact agttctgtat ctccaaagga taagctgtaa tcgaaaaaaa taaacgacaa
     3661 tagcacttca ttttttccag tattggttat ccaatgatga attttggttt tataatcact
     3721 aagactcata caccaatttg ggttagaagc catcatttcg gcaggacaat aatcataacc
     3781 aatttcaaaa agtcccttgt tgatgcgggc tgatagttct aaaaaatatt ttttagtttc
     3841 tgcttctaat tcttctggga catcttcata aattaaagca ttatcttgat cggttaataa
     3901 gagttgttcg cttcttcctt gacttcctaa tgccaaccac gaaaatttta caggaggaga
     3961 agtttctgtt tttttcaata caattgcaat aacttgtttg gtacaagcat cattcaattc
     4021 agaaataatt tttgtaatca atgtcatcgg tatattttga tccaaatacc cttgcaataa
     4081 atgtataata ctagctcgaa tttgttttat ttttttaaac ttcttagctc tttttatttc
     4141 ccttagcaaa acggcaggat tattaccaat agaaaccatt acatcatgtt tggataacat
     4201 tccaacggct ttcgaattgg aagttccatc tatggtaagg cataaatggc taatatcact
     4261 tttcatcatc gctatttgcg cttgcgttac ggtcattttt gtgggataag taatcacagg
     4321 cgaagtcatg atttcagaaa ctggagtggt aatttgaaat tcaccggtta caattttgtt
     4381 tcttaaatct ttgtcagtga tgatgccaat gggaaacgtt tcttccacaa caagaatggc
     4441 tcctacattt ttatgacgca ttatttcggc tgcttctttt gctgttgttg ttgcaataca
     4501 actaactatt ttttttgagt atttaacttc ttgcaaatcc aatagttttg tatcggcttc
     4561 aagagattct tcaccatata atttgcctct atggctttta gaaaaagggt ttaatgtgtt
     4621 tgaagcaaag ctttcgatta aaaaattccc aactgcttta ttttcaagcg catatggttt
     4681 aaaaacactt ataggaatag cataaagtat gctttcttca taagtccggg cttccatttt
     4741 gtagttttca ttggctatca aaggtcttaa tccaaagata tctccctcat cacacatatc
     4801 caaaatttca ttttcatggt ttcctctcag ggctacagct cctttatgaa ccacataaaa
     4861 actgttgtgt gtttcttcgt tttcggcaaa aactacactg tctttttctt tataaataat
     4921 agttatttgc tcggaaatga gttccaaatc tctttgtttt aaaaagctaa acggagggaa
     4981 gttttttaaa aagtcggcaa ctctttgtga gattgtgttt ttcatgaagt ttatttattg
     5041 caactaaatt acattttatt tggaataaaa aaagccccaa atggagcttc aattacatat
     5101 tcttatttat tttttttgtc cactttcata ttcaaaaatt ctacaaatag cgaaaaggaa
     5161 atggcaaaat acaaataccc tttttgaaca ggtgtaacat gactaccaaa gattaaggca
     5221 ttcgataaat gggcactttc agtaattaac ataaaaccaa ttaagattag aaaggacaat
     5281 cccagaattt gaatagaagg atttctgttg acaaatgcac ctactggaac ggcaaattgc
     5341 atcataatta ataccgaaat aattacggct gcaatcataa tataaagggc gcctgaaact
     5401 ccatttgtca ttcctaccgc cgtcaaaata ctgtcaaaag aaaaaaccaa atcaatcata
     5461 atgatttgaa gaagaactcc ttgaaaggat tttgaagcgg ttttttttat agttttttct
     5521 tcttctcctt tttcatccac tttttcgtga atttcagtag tgcttttgta aatcaagaac
     5581 aatccaccta ataataatat taaactttgt cctgtaactc cagctgaaaa ccaactaaaa
     5641 tcgatataaa accaaggttt tttcattccg attaacaaac tgattccaaa caacaatgca
     5701 attctcatga acatagctag aaacattccc acttttgtgg cttttttgcg tttttctggg
     5761 ggtaatttcc ctgtaactat cgatatgaaa ataatgttgt ctataccgag aatgatttcc
     5821 agaaaagtca atgttaataa ggcaatccaa gcatcaggat ttaaaaatac ttccattttg
     5881 ttttaatata agttataatt tttaggcaat ttaggcttct agcttcctgc ttcaattttc
     5941 cattttctcc acagttccgc gttgcttttg gtcactgccc acaattccga attcaaaagt
     6001 ataggaattt tttgaagtag tcaaaatttt catagtaatt gccttttttt ctgccctatt
     6061 cttcggatgg attttttgca aaatatattc gcaatcgctt acccatcgaa tactggaagt
     6121 gtcggtttta ccttcaaaag tttcaatttc gatactgtct tttcgttcaa aaacggttac
     6181 tttttttgct ccatcaactt catattcaaa tttgaatttc ccggttttga aatctttaca
     6241 attgtgttct acattataac acgacagtag taatagtagc gtgaggagtg agattatttt
     6301 tttcattgtt gttttagttc agtttattca actcatcatc cgtaaaattt cgaatacttt
     6361 tttctttaga aaatttttca gagttgtagg cgttggattt gttttttgga atggataaac
     6421 ttgtccattc gcttttcttc agtattttgg catagaaaac tatttgtcca atatgatacg
     6481 gataatgtgc taattgacga tttatagctt cgattacggt atgtccttcg tttctaatat
     6541 agataatcgt cgaaagttgt tcggtttgta aagcattcaa agtatcaaaa aaacaattcc
     6601 aacctttttc ccagaggact aataattttt ctttggtttt tatggtatct tcaaactctg
     6661 catcgcgatt tcgccattct ttctcgccat ccgaagttag aaaatcagtc caacgcgaaa
     6721 gcatattgcc cgaaagatgt tttacaatcg tagcaatact attggtttca tcatttatgg
     6781 aaacaaaaag ttgctcgggt tcaagttggt ctatcgcttt ttctcccaat gttttataat
     6841 atagaaattg cttttttaca ctttctaaat aagaatttgt tgcttccatt attactctat
     6901 tttaatgtca tatttatcca aaagtccaac gattccttga gtagaaaact tggcttttcg
     6961 cattggatta aattcgggat caattttaaa attttgagct gttctaaaat caactccagc
     7021 caattgagta tcgttaaaaa tagcattatc caaattacaa ttttcgaaac tcgaattcgt
     7081 gagatttgtt ccaataaaag aaacttcttt catcgaacaa gtattgaact tagtttttgg
     7141 cattttcttg ttggcaaatg acgaataatc caaaagacag tcttgaaaac tcaccccaaa
     7201 cataaaatca gcacaggaat taaattggat tcctaataat ttgcaggttg taaaatgcac
     7261 tgttttcaaa ctcgttccac ttaattgcat catcgagaga ttgcaatcaa tgaactcgca
     7321 atccataaag gtgttgtttg agaaattact attcgagaaa tcgcagttct tgaaaatgca
     7381 atcttcaaac tctcgattgt tgactttttt gtccgtaaaa gatatttttt cgaatgtttt
     7441 atgggtgtgt attaggtttt ccattagtca ttgaataaac ttcgcatcat tgtttttaac
     7501 cccaaaatca tcagataaac agcacccaaa caagtaagaa ttccaatccc taaaaccaaa
     7561 taatgccaaa cattttgctt gttgatgaac gcattgtata tcaaggaagg accgataaaa
     7621 agcaaaggca aagctcctgc taaatattta attcctttgg ataaaagttc tttattggtt
     7681 gccattttta tagtttgctt ttataaaaaa ttactgcttt gcgaacactt ccgtgagttt
     7741 ttagcaattc ggcaccttct tcataggaaa cattaatttc gcccataatc attttcacac
     7801 cacgatccac gagtttgcta ttgctcaatt gcatatcgac cattttgttg cctttcactt
     7861 ttccgagttg aatcatggtg gcagtcgaaa tcatattcaa gactaatttt tgggctgttc
     7921 ctgctttcat tctcgaactc ccggttacaa attctggtcc aacaattact tctattgcaa
     7981 attttgccgt ttgcgacatt ggacttccag cattacatga aatacttcct gtgagaatgt
     8041 tatttttgtt acaagcttgt aaacccccaa tcacataagg ggttgttccc gaagcggcaa
     8101 ttccaaccac aacatctttc tcgttgatgt tttgtgcttt caaatctagc caagcttgag
     8161 tggtgttgtc ttcggcattt tctacggcac gacgaatggc tttgtcgcca ccagcaatta
     8221 ttccgttgac catgtcgaaa ggaaccccaa atgttggcgg acattccgaa gcatcaacaa
     8281 cgcctaaacg gcctgaagtt cctgcaccaa tgtagaacaa tcgtcctccc aatttcattt
     8341 ttgcaacgat ttcggtaact aaagtttcga tttgcggcaa agctttttct acggcaaaag
     8401 gtacggtttt gtcttcttga ttgatattgg atagtaattc ctgaatagac attttttcta
     8461 aatgttcgta tttcgaggat tgttcagtag tttttgtaaa ggtcattttg atgcgattct
     8521 aatgtgtgaa atagacacgt aatttttttt tgcaaactta aataaattaa aggtagcaac
     8581 cgtgtataaa taaaaattta gaattgaatt ttcataagaa ctaatgttaa agcaaattgt
     8641 tttggcatca aatctgctcc aaaaaaaaga attagtaatt aaaaaaatgg tgcgttttaa
     8701 atgtgaataa tagactcttg ccaagtagta tcattagagt atagttttaa aatatagact
     8761 cctactggta aatttgcaat attggtttcg tttataccta attgtaattc cttaattttt
     8821 ttaacaactt gacctgatga attgattaat tcagctttga aattgcctcc attaggctct
     8881 ttaatttcga taaaaagagt atgttttgca ggatttggcc aaaatttgaa tgcaggctct
     8941 ttttcaaggc ttgccactga aagagattcg tttaaagtga atttaagaat agctgctgga
     9001 ttttgcataa cgcttacagt attcattcca gaagcatcag cggttttacc agaatcatca
     9061 gtaatcagat aaaaggactt gccgtctgga gcagcaacaa tatcacgata acgattttga
     9121 gtataaaaat actgtattgc attacctaca acagcggttc cggttgcatc caattttact
     9181 ctataaactc tgcctttttt taaacttgtt agcaataacg aatttttcca cgctggaata
     9241 gcgtcacttt catatatttc taaactagat ggagcaatat tggggcggca catccaagaa
     9301 tcagtgcagg cggagtcttt gtaggtgtta gctagaaaca gtgacataat tgggtcttga
     9361 tagtttgtag cggtgaatga agtttcggta gtcagacagc catcggcatc atacattaaa
     9421 ttgtctttta cacctgccac aaagggccag ccataatttt ttccagaatt gataatgtta
     9481 atttcatcat cggaactggg tccttgttca tcagaatata atattccgtt actaccgaaa
     9541 acaagccctt gtgcgtttct gtgtccataa gaataaatgt gactttttac accatttagt
     9601 acaggattat cagttgggat tgagccatca agattaagtc ttaaagtttt tccaggataa
     9661 tttgcccaat tcttctggtc tatttgtagt tgtgttggta aaaattgagc caaattagag
     9721 gcacacactt tcacggcttg atctcctatg gtgtaataca gtttttgatc aggaccaaaa
     9781 ataagtctgc ctgattgatg gtcattacta gaaggcagat tatcaataag taccacaggc
     9841 gaatataaac tgccatcgtt tccattaata gtatatgtat atcttactat tttttgtttg
     9901 ttttgtcccg atacaagatg cgtataggat aaataaacat aaggactagt ggttagaaaa
     9961 tcaggatgga gtgccattcc gagtaaaccg tcttgacccg tggctgttga acttaaatct
    10021 gaaagttgaa tcaattcatc tcgttgtgct gttactggat ttactctaac aacacccact
    10081 cttttttcag taacccagag atatccatcg ggtccatact gtaaatccca cggaactttc
    10141 aataaattat tggcgccaat ttgtgtcatc gtaaaagttt cattttgcga atgagaaaca
    10201 ggtcctatta aaataattaa taagacaaaa agaacacgca atcgattgaa aaagttcata
    10261 aaattaaaaa ttagaataga tgataaaatg tttgcgaaaa atacacaaag actgcagaat
    10321 gttaacagaa tttattaata ttctttcaaa ttcatttatt aattgtgtta attatatttt
    10381 tttttgggaa attaacaatt aaattttaat aaatagttga attggtatta aaaatttaaa
    10441 aactattatt taagaagaga cagtaggata atgaacaatt ttaaaattgc aagaattggc
    10501 tataaaacaa agttcgtttg ctttgacatg caaatttatg gcttcatcta ttttttctga
    10561 ctgctgaatc acgacttttg gatacaagcg tacttcaata aaacgtcccg aaccattttc
    10621 agaaacttcc agtgttgcct cggcatcatc ttgataggaa agcacatcaa tgccattttg
    10681 agaacaaaca tacaaatacg acatcatatg acaggaaacc aaactgctca acaacaaatc
    10741 ttcgggattg tataaactag gatcgccttt gaatgctttg gcagccgaaa ccgttaaatt
    10801 ttcttttcct tcaatagtaa tttgatgggt tttaaaatat gtttttgtgg ttgctattgt
    10861 ttctttggaa acagaatccc aagttaattt tgctttgaat atgtggttga aagacatatt
    10921 gtttggagtt ttttttggga atgatgtaga tttagtttag cgaatttggc tccgcaaagt
    10981 ttataacttt gaggatgtgt ttttaagtct gtgactgaaa taaatattat ataaaaaatc
    11041 ggttaaaaac cgagaatcac gaactcaaag ttataaactt tgcggagctt gattttattt
    11101 ttcaccaatc caaacatcac tacttttaaa taaatatatt ttttctgcaa ttccgtttct
    11161 tttttggtcc attattctgt gaggcttatt taattgattt ttcagccatt tttgttcttc
    11221 taaataagct gcttctgaaa taaaattttc tcttaaaaat tcattttcaa atattgggtt
    11281 ttcttcaatt ctatattccc aatcataatt atttattcta ttagagattt tgtccaaact
    11341 tgatttcgat attttttttg ctatttctac ttcaagaaaa atattttcat aatattcaat
    11401 tttttcattt attgcacaaa ctacttgtga attataaata tcaggctcaa ataaccatat
    11461 ttttaaataa tatggtttgc caagttcttt caattgtata ttccaacttt cataaatatc
    11521 aaaaaacgat tctaaaattt tttttctaat tttccctttg ggctttggaa aattattcaa
    11581 tgacattgca actggattcc aggggaatat tctaaatttg atataatctt tattggattt
    11641 ttgtaaaaaa ttaaaatcaa tttccagact attaattcgc cattgttcaa ttttatttaa
    11701 ccgtctttta tttcctctta tttttttcat taataaaata tgctctaaat attggttttt
    11761 attcttataa aaaaacaatt tttaaataaa atacgccaac acaatcccaa tcacaatcat
    11821 cgaaactttg gcaatattaa atttgtgtcc ttcgctactt tcgaaaataa tggtggacga
    11881 aatatgaaac aatattccaa taactacagc ggtaatttcg ttataatagt tatttaatat
    11941 gggcaaataa tccgataaaa tagtgcccaa aggtgtcatc actgcaaaag taatcatgaa
    12001 gaagaaaatg gcttttttgt cgagttgggc attaataaaa aacattgtca atatgatggc
    12061 aataggcaaa tgatgaatgg caattcctaa cgccaagttg tgatggtgac ttacgggaaa
    12121 accttccaga aaagcgtgaa tacacaaact gacaaacagc aaccacggaa tgtgggacat
    12181 cgtttcgtga ccgtgaacgt gaccgtgttc ggctcctttt gagaaaaatt ccaaaataat
    12241 ttggaacaaa attccagcca taataaaaag tccaatattg gtattgtgac tttcataaac
    12301 ctccggaagc aagtgcatca cggttagcga aagcaaaaaa gaaccactaa aagccaacaa
    12361 taatttaagc ttggttttgc ttttgggttt caaaaacaaa gcaattccat aacctaaaag
    12421 aactgaaaat aagggtaaga ggtaattcat tatttttggt ttatgggttt aaaagtttaa
    12481 gagtttaagg aatgtttgag agtttttgtt ttaattaatt ttaaatcctt aaattatttg
    12541 aatatcataa tcaatcgttc gctttcggtt ttgtggaatt ttttcagttt gtaatctcca
    12601 aaaatatcca acaaatagat gccagcttct tccatcattg cttcaaagtc ttttagagtc
    12661 aaagctttta ctttttcggt aaaatgaaat ttttcgcctt gatcttcaaa gtctatttct
    12721 ttgtaaatgt gtccatcatt gacataccgt ttgatgtgaa attcaattcc atcaaccgtt
    12781 tttacttctt cgggaactaa attagcaatg acttgattga cgttcataaa gtcgattaca
    12841 gcaaaaccgt gttcagtcaa actttcttgc attgctttta gcgaagtcaa attgtcttca
    12901 tccttttcga aatagccaaa actggtaaac aaattgaaga tagcatcaaa tttgtcttca
    12961 aatttttcac gcatatcgtg aacttggaaa tgcaagttgt cgttttggtt tttattggct
    13021 tcggcaatgc tgttttcaga caaatcagca ccaataacat caaatcctaa ttggtttaaa
    13081 taaatagcgt gtcggccttt gccacagcac aaatccagca cttttgcctt ttcgggaagg
    13141 ttgaggtaat gggtcaaatt gtccataaaa acctgagctt ctctgtaatt tctttcttta
    13201 taaagaatgt gataataagg agtgtcaaac caagaggcaa accaattatg ggttttggga
    13261 tttagtgaat tcggcattta ttctttttct attaattcaa gtctgcaaat ttagtgtatt
    13321 tttgtaccga atacttgatt ttacaatgga aaataattat agaatgattg ccaaaacctt
    13381 ttttggtttt gaagaaatac tagccgaaga actaaaaaag ctgggcgcac aagaggtaga
    13441 gcaaggcgtg agaatggtga gcttcaaagg agataagggt tttatgtaca aagccaattt
    13501 gtctttgaga actgccttga aaatcctgaa acctatttat tcttttaaag cgatcaatga
    13561 acaggctttg tataaaggca tttcaggtgt aaactggtcc aaatatttga atgccaatca
    13621 gacttttgtg attgatgcca cggtgcattc tacttatttt aatcattcgg aatttgtttc
    13681 ccaaaaatgt aaagatgcca tcgtagatca gtttcgcgaa agaacaggtc agcgtccgag
    13741 tattgagaaa attcatcccg atttgcgaat taatattcac attgacaaag accaagtttc
    13801 agtagcatta gacacttccg ggaattcgtt gcaccaaaga ggctatcgat tagcgactaa
    13861 tattgctcca atcaacgaag ttttggcagc gggaatcttg ttgctttcgg gttgggacgg
    13921 tcaaaccgat ttcttggatc caatgtgtgg ttcgggaact tttttggcgg aagccgcaat
    13981 gatagcttgc aatattccag ccaacattaa ccgtaaagaa tttgctttcg aaaaatggaa
    14041 agattgggat aacgatttgt ttgaccaaat tatggattct cttttgaaaa aagttaggga
    14101 atttcatcac accatcaaag gatatgacaa agcaccatcg gcagttatga aagccaaaga
    14161 caacatcaaa aatgccaatt tggacgaata tattactatc gaagaaagaa acttttttga
    14221 taccgaaaaa acattagaag ggaagttgca tattgtattc aatccgcctt atgacgagcg
    14281 attggatatt catatggagc aattctataa gaatattggc gacactttga agaaaaatta
    14341 cccgggaacc aacgcttggt ttattactgg aaatctggaa gcattaaaat ttgtgggact
    14401 gaaaccatcc cgaaaaatca aattattcaa cgccagtatc gaagcgagat tagtaaaata
    14461 tgaaatgtac gaaggaagca aacgaaccaa atttcaaata acagatggca ggtagagacg
    14521 caatgcattg cgcctctttt gcatataatt cataaattta aaaaaatgac aaaattacaa
    14581 gttcgcgctt ttcttttcca attgggatgt tttgcgatat tatttatttc tttccaatat
    14641 ttgattggaa aatataccaa tttggaggga ttgtggattc agtttaccgc ttttgtggtt
    14701 ggaaccattt taggaccaaa atttcaggct gcgaaaacta aagaaggcga aaaattgttt
    14761 atgtcttggt tgtttataaa aggaattaag gatattactt aatttctttt tttactactt
    14821 ctttcggttt tactgttttc ttatacaaag gaataaaata agtaacggta tagttaaatc
    14881 cagctccaaa atttccagca tatgttctgt tgaagcctgg tatataaagg ttttcgaagt
    14941 tttcgtctgg tttcttttcg aaaagtagcg tgttcattcg aagactaaag cccacaaata
    15001 tattgttata cacttttact ttcacaccag caacaacttc tatccaaccg gcagttaaac
    15061 cgttaaatga ttcaccagaa gcaatccaag gcatttcatc ccaataatgt tgtgtgttgt
    15121 agattttgta actgtttaat tcttggttaa aagtactgaa accgcctcgc aaaccgatgg
    15181 agataatgtt ttccatattc agccagtttt cataggcatt gtaatcaaat ccaaatttta
    15241 tgtaagttcc tttggtagtg gtgtttaatc ggtcgtcttc ggtggttttg tcttcgcttc
    15301 caatttcggc agccaaatag taatttttag taaaacgata atcacccacc aattcaattc
    15361 ctttgtagtt tgaatcatat agtcctcgag tcaatttata caaatccaat ccaactctaa
    15421 cgccataacg atcctttttt acagggacaa cagtgtcggt ttttgtagct atagctggtt
    15481 ttgcttgtgc ttgttttggc gatgcaggaa taattccttc ggttttcgtt ttatctagcg
    15541 acttagtagt tggagtagtt tcttgtgctt gaatacaaaa cacagtcatc aaaagtccaa
    15601 tactaaaaaa atattttaac atgtgtttca ttttcgttgg ctatgttact tttttcgatt
    15661 gaaatgaccc gcatccattt tgtttttgtt gatggtactg ctgaatgaat atagggattg
    15721 gttggatcaa aagtaaaaag ggttttaaaa ccgcaagctc ttgatacaaa aacattttct
    15781 ctagtataat taaaagttac ttcatcaata tcaatcaagg taggatttgc attgcccgaa
    15841 ttgagagtga aactaaattt ggttgaattt gtatctgttt ttagtgggat tgaaatggag
    15901 gttccattag tcagatattg gtcatcaccc gtagcacttg aattaaaaac aattccttct
    15961 ttcattccat ctccaacaac ttttagattg gtcacatttt tcttgacaga tggagtggta
    16021 atgttataaa aatcaatcac caatcgggga gttgtaggtg tattggcatc gcaaatatcg
    16081 tctttctcgc aactcgagga agtgacaatc acaagcaata aaatcgaaag tatttttttc
    16141 atattatttt aaatggcaat tattatccac taaattactg aaatctaaaa tctgctcccg
    16201 aaacttcggg actgtaatct attttctctt ttctaaaagt acaacatttt ctacgtgatg
    16261 cgtttgcgga aacatatcca caggtcgcac tcgggtaact ttgtattttt cgtccattaa
    16321 ggccaaatca cgagcttgag ttgccgaatt acaactgaca taaactactt tttcgggttc
    16381 gattttcagt atttgttcga tcacgtcttt gtgcattccg tctcttggtg ggtcggtaat
    16441 aataacatcg ggtttgccgt gttgagcgat gaaactttcg ttgaaaacca ctttcatatc
    16501 acccacatag aattcgcaat tcgttatatt gttgcgttca gcattggctt tggcatccaa
    16561 aattgcttcg ggaacgcttt ctacaccaat cacttttttg gcttttttag atacaaattg
    16621 agcaatagtt cctgttccag tatataaatc ataaactgtt tccgtgcctg ttaatccagc
    16681 aaaatcacga gtaattttat acaattcgaa tgcttggtca gaattggttt ggtaaaaaga
    16741 tttagcgtta atgctaaatt tcaaaccttc catttcttcc aaaatgtaat cacgaccttt
    16801 gtataatttg atgttttgat cgtataaagt atcgtttgct ttattgttga tcacatattg
    16861 caatgatgta atctgtggga atttctcgta gagatgatcc aaaagtaatt ctctaccagc
    16921 tttatcattt tcgaaaaact gaatcaaaac cataatttct ccagttgaag cagttcgcat
    16981 catcaaggtt ctcagtaagc cttcgtgatt tcttggattg aaaaaagtca ggttgtttgc
    17041 attggcgaaa gccctgattt cattacgaat ttggttcgat ggatcttcct gtaaatgaca
    17101 tttgttgatg tccaaaatct tatcccacat ttttggaata tgaaaaccaa gggcatttct
    17161 gttgcccaaa tcttcggtgc tgccaatttc tttttcggtt aaccaacggc tgttcgaaaa
    17221 cgaaaattcc attttatttc tgtaaaagaa ttgtttttcg gatcctaaaa taggttcaaa
    17281 atcggggagt tctatttttc cgatgcgttg taggtgattc tgaacttcat tttgtttgta
    17341 gtacaattgc tggctgtatt tcatattctg ccatttgcaa ccgccacaaa caccaaaatg
    17401 ctcgcaaata ggttcaattc tatgctcgga atattcgtga aatttcactg cttttccttc
    17461 ataatacgcc ttacgctttt tgaaagtttg cacatctact acgtcacccg gaactacatt
    17521 gggaataaaa attacttttc catctggggc ttttgccacc gaaacgcctt ttgcacctgc
    17581 atcaaggact tttatatgat caaaaacgat cttgtctgta tttttcttag ccatagcgca
    17641 aaaataaggc tttgaatggt tttataaatt caaattggga aaaatttttg aaaagatgtt
    17701 gatttaatga aattattctg tggtaaaagc tttttgcttg aagtttgaag aagttattgt
    17761 ttccatcttt tttgttgcag ttcattactt gagggtttag ttgtatttag agcagaggtt
    17821 ttaccgaatg aactataaat aaaaataaaa aagtgagctt aatcaacata aggatataga
    17881 aacaaatttt tacagttgtt tgtgtttttg ttagtagaat agagtttttt ttaaataaat
    17941 gactgtaaaa taggtgttct tttgtgaaaa accataaagc aatgaaagtg taattgttta
    18001 tatttgaaaa atattattct agtacaaatt
//
LOCUS       DAOSNN010000019        17798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3202_length_17798_cov_4.734600, whole genome shotgun sequence.
ACCESSION   DAOSNN010000019 DAOSNN010000000
VERSION     DAOSNN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17798
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3202_length_17798_cov_4.734600"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLQ70_02015"
     CDS             <1..205
                     /locus_tag="PLQ70_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQE33420.1"
                     /translation="SKNIALSSVVYLNSFLTIAMRNKIQKIIPFVAVIIGIMFIFRGL
                     GLGIPYLSPSNMSLFVQSSPNCH"
     gene            230..460
                     /locus_tag="PLQ70_02020"
     CDS             230..460
                     /locus_tag="PLQ70_02020"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HQE33421.1"
                     /translation="MENQDLLHVSPDVQEKIEKLITENDHFAALFEEYRKVKQEVASI
                     KSEVVVTTDEHLKELKVKILHLKDEIYSILRR"
     gene            complement(514..1878)
                     /gene="hemN"
                     /locus_tag="PLQ70_02025"
     CDS             complement(514..1878)
                     /gene="hemN"
                     /locus_tag="PLQ70_02025"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HQE33422.1"
                     /translation="MKNSILQKYNVPGPRYTSYPTVPYWDESDFTYQNWIDTLKRSFT
                     ESNTEEGLSLYIHLPFCESMCTFCGCNKRITKNHDVEHPYIEAILKEWAIYCNILEEK
                     PIIKEIHLGGGTPTFFSTKNLEDLINGIFCYAEKAKDHEFSFEGHPNNTTRAHLQKLY
                     DLGFRRVSFGVQDYSEKVQKAINRYQPFHNVAKVTFWAKEIGYTSIGHDIIFGLPFQE
                     IDDVIDTIEKTNSLQPDRLAFYSYAHVPWIKGNGQRGFNDEDIPKDEVKRMLYEVGKD
                     LLFENEYYEIGMDHFALKTDTLYESFEQKKLHRNFMGYSSSKTQLMIGLGVSSISDSW
                     YSFAQNVKELEEYYKLLKADKLPIFRGHLLTPEDLIIRRHILNLMCHFETSWKDKTQY
                     FDEVPEILIQIKEMEKDGLVLIEKNGIKVTNAGKPFVRNICMAFDLRLKRKAPDTKLF
                     SMTI"
     gene            complement(2020..4062)
                     /locus_tag="PLQ70_02030"
     CDS             complement(2020..4062)
                     /locus_tag="PLQ70_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HQE33423.1"
                     /translation="MLEEKNDNLLEADGNLENHSIETPLVEENAEIVSETIAEVEKET
                     VLTPEVSVEDTIEMAKPIEEESTEVAEAKASETETIIDAIAENNAEESEDETLKERHD
                     IPMLDYDALSLESLVDELKKLVTNEKVMSVKDHVEEIKKSFLAKYNHLLEEKKEEFLA
                     ENPDTTEEFAYHSPLKSQFDQYYSLFRDNKNAHFKSLQTNLKTNLENRLAIVEELKEL
                     INPQENIKDTLKHFNDLRERWKNAGSIPKDKYNHVWNNYHFHVENFYDYLHLDREARD
                     LDFKHNLEQKQKIVARVEELINEADVNKAFRELQDLHKIWKEDIGPVSREFRDEIWNK
                     FSDLTKQMHDKREVLFEKMRGTELENLEKKKEIIAKIEVLATEKVNAHSQWLQQIEKV
                     EALRTAFFSAGKVPAELNEETWATFKTAVRNFNSFKNSFYKDIKKDQNNNLNRKTALV
                     AKAKELQESTDFATTTPIMKQIQEEWKQIGHVPRKYSDKIWIEFKDACNHYFDKLKEQ
                     KNEENSEEIEAFDKKKAYLETLREFQLTGVHKTDLDAIKLHIETWKGFGKVPFTRRHI
                     EGKFNKVLDALFEKLSLSKKDSEMMRFSNRMDQLSENNDTRKLEGEKIFLMRKIDEIK
                     NEIFQLENNIQFFTNTRNAKKENSIVLEVRKNIEKHKEEMEILKDKLRQVRSLNQS"
     gene            4386..5240
                     /locus_tag="PLQ70_02035"
     CDS             4386..5240
                     /locus_tag="PLQ70_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQE33424.1"
                     /translation="MRPILPINKSEILKRYKPINPNTHKGIQGHALLIGGSYGKIGAI
                     SLASKACLKTGCGLVSVFVPRCGYKIIQTAVPEVMVLNDVSVKYITRIVFDFVPNAIG
                     IGPGMGQDIETHTALHRFVKTNKVPLVIDADGLNILSQNKQWLTFLPEKTIFTPHQKE
                     LERLIGQWDSFADKIEKTIAFSKQNNLIMVMKGAPTFIIDGDTIYENTTGNAALATAG
                     TGDVLTGMITSLLAQSYDPIDAAILGVYLHGLTADIALPETGYQSFIASDCIANIGKA
                     YLSLEKIE"
     gene            5366..6178
                     /locus_tag="PLQ70_02040"
     CDS             5366..6178
                     /locus_tag="PLQ70_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33425.1"
                     /translation="MNFISNRTIIIKSLIAAIAILGIKNFVFKNLSLYGQDFHDLIEL
                     SDITIIFTGAFFVFGLLLAATMTDFKESEKIPGEVASNLEAIKDWIYLAFKAPRTGTT
                     ELCKEELNTAFLRQEMVDITEGIIAWIYSSEKESNVIFPLIRKSNEIAYYFAERGVDK
                     EAIKGIQENTNAMRKQLTRAYSISRNNFIKPAYTLLQSILFIVMSLLLITKFKSPSAD
                     YLVTSAITFLFCYLYLLISGLDDPFDVFNGDTNVDLKPIDRFKQRLLSDFLV"
     gene            6619..9120
                     /locus_tag="PLQ70_02045"
     CDS             6619..9120
                     /locus_tag="PLQ70_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQE33426.1"
                     /translation="MTTTYTITGMSCNGCRTKVEKALNEIDGMEATVTLDPPVATIVS
                     EKPISVFKLQKAVYVVGNYIISESNASHENSLYSTPIPSEMKPVDLPQSASGKYYCPM
                     FCEGDKVYDEQVGCPVCGMDLVKAPELMAAKTQYTCPMHPEMVQDQPGNCSICGMDLV
                     AIQAAEEEDSAYLDLWRKMKISLIFTIPVFIISMLEMLHNNPLLELMETQKWNWVQFI
                     LALPVVFYAGWMFFVRAWKSILTWNLNMFTLIGIGTGVAFLFSVAGLLFPQFFPEEFK
                     HRGTVSLYFEATVVILTLVLLGQLMEAKAHGQTNKAIKELLKLAPTQATLVKNGEDKV
                     ISIHDIKKGDVLRVKPGEKIPVDGKITEGESTIDESMISGEPIPVDKTIEDAVMAGTI
                     NGNKSFVMLAEKVGSETLLSQIVQMVNDASRSRAPIQKLADSIAKYFVPTVVGIAILT
                     FIIWANFGPEPAMVYGFINAVAVLIIACPCALGLATPMSVMVGVGKGAQSGVLIKNAE
                     ALENMNQVNVLITDKTGTITEGKPSVERVFSSNNEEDNVLQNIASLNQYSEHPLAQAV
                     VNYAKTKNISLMEVKDFEAISGKGVIGTVANKKIALGNKKLMEQVNASVSDDLESKII
                     AEQKLGKTVSYVSVDGIAVGFVSISDKIKTTSADAIKELIRQGVEVIMLTGDNENTAK
                     SVAEQLHLTRFIANCLPEDKLNEIKRLQAEGKIVAMAGDGINDAPALAQANIGIAMGT
                     GTDVAIESAKITLVKGDLQGIVKAKKLSQAVMKNIKQNLFFAFIYNVLGISVASGMLY
                     PVFGLLLSPMIAAAAMSFSSVSVILNSLRLRSFKM"
     gene            complement(9255..9764)
                     /locus_tag="PLQ70_02050"
     CDS             complement(9255..9764)
                     /locus_tag="PLQ70_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HQE33427.1"
                     /translation="MNPYLDIILRSASVYLFMLIALRIFGKKELSQLNTADVILILLI
                     SNSVQNAMVGSDTSLLGGLAAAAVLFIINFTFKRLIFKFPKFSDFMQDKPEILIHEGK
                     LDFKTLSKLKITSEELQEAIREHGVEHFSDVKLAMLEIDGNISIISDQNNLRQTHYKR
                     LKRSKKTMG"
     gene            complement(9830..11200)
                     /gene="rimK"
                     /locus_tag="PLQ70_02055"
     CDS             complement(9830..11200)
                     /gene="rimK"
                     /locus_tag="PLQ70_02055"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HQE33428.1"
                     /translation="MLQSKIILGSEEWCSLPELGIPTIKARVDSGAKTSALHAINIAP
                     FKKESQNWVKFDINPIQNNVKTVIHCEAPLVDKRIVKSSSGFREERYVIQTNLEIGNS
                     KWVIEMTLTNRDSMGFRMLLGREAMSGRVMVDPEQQYLLGEPTSESLKEVYKNAKKAS
                     TGIRIALLASNPELYSNKRIMEAGEMRGHEMHFLNIKECYMKLDAKTPEIHYRGGLIL
                     NQFDAVIPRIRPSITFYGCALTRQFEAMNVFCLNSSTAITQSRDKLFSLQLLLQSGIE
                     IPTTGFAHSPLDTDDLIKMVGGTPLIVKLLEGTQGKGVVLAETKKAAESVINAFKSLN
                     ANILVQEFIKEANGKDLRLFVIDGKVVAAIQREALAGEFRANIHLGGTASVIKPTAEE
                     KKIAIRAAKAMDLKVAGVDIIRSSKGPLLLEVNSSPGLEGIESATNKDIAGEMILAIE
                     KNFKLN"
     gene            complement(11389..13935)
                     /locus_tag="PLQ70_02060"
     CDS             complement(11389..13935)
                     /locus_tag="PLQ70_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQE33429.1"
                     /translation="MDDNFSPRVKDVITYSKEEALRLGHDFIGTEHLMLGILRDGNGK
                     AINILNNLSIDLNLLRRKVEVLSPASPTMDSNNEKKNLHLTRQAERALKTTFLEAKVF
                     HSTSISTAHLLLCILRNENDPTTKLLNKLNIDYDVAKEQYLNMTPSEENFIENLPKND
                     AYNEDSGQDDSLKEGNFNNPANKSNKKSKTPVLDNFGRDLTEMAEEGKLDPVVGREKE
                     IERVSQILSRRKKNNPLLIGEPGVGKSAIAEGLALRIIQKKVSRILFNKRVVTLDLAS
                     LVAGTKYRGQFEERMKAVINELEKNDDIILFIDEIHTIVGAGGATGSLDASNMFKPAL
                     ARGEIQCIGATTLDEYRQYIEKDGALERRFQKVIIEPTSVEETITILNNIKDKYEDHH
                     NVTYSPEAIEACVKLTDRYMSERFLPDKAIDAMDEVGSRVHIINIEVPAQVLDLEKQL
                     EDVRELKNAVVKKQKYEEAAKLRDDEKRLEKDLAIAQEQWEEDSKNNRIQVTEDNVAD
                     VVSMMTGIPVNRIAQTESNKLAILPELIGNKVIGQNEAVKKIARAIQRNRAGLKDPNR
                     PIGSFIFLGQTGVGKTQLAKVLARELFDSEDALIRIDMSEYMEKFAISRLIGAPPGYV
                     GYEEGGQLTEKVRRKPYCVVLLDEIEKAHPDVFNMMLQVLDDGYLTDSLGRKIDFKNT
                     IIIMTSNVGARQLKDFGQGVGFGTAAKVAQADDNSKSIIENALKKTFAPEFLNRIDDV
                     IVFNALEKEDINLIIDIELKKLYARIKDLGYNLILSDKAKAFIADKGFDRQFGARPLK
                     RAIQKYVEDALAEEIITSKIGAGDEIFMDLEDETSQELSVKIQKAEEPTN"
     gene            14197..16851
                     /gene="gyrA"
                     /locus_tag="PLQ70_02065"
     CDS             14197..16851
                     /gene="gyrA"
                     /locus_tag="PLQ70_02065"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQE33430.1"
                     /translation="MSEGEKLIPINIEDEMKSAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLYGMYDLGVNSRSAHKKSARIVGEVLGKYHPHGDTSVYDAMVRMAQEWSMRYLLV
                     DGQGNFGSVDGDSPAAMRYTEARMKKISEEIMADIEKETVDFQLNFDDTLYEPKVMPT
                     RVPTLLINGATGIAVGMATNMPPHNLTEVINGTLAYLDNNDIEIDELMNHIKAPDFPT
                     GGVIYGYEGVREAFKTGRGRIVMRAKVGFEEVDGRECIIVTEIPYQVNKADMIKRTAD
                     LVNEKKIEGISNIRDESDRNGMRIVYILKRDATPNVVLNTLYKFTQLQSSFSVNNIAL
                     VKGRPQMLNLKDMIHYFIEHRHDVVVRRTQFELRKAEERAHILEGLIIASDNIDEVIA
                     LIRGSKNTEEAREKLIERFQLSDIQARAIVEMRLRQLTGLEQDKLRAEFEELMKLIEH
                     LKALLADVDLRTALIKEELEEIREKYGDARRSQIEYSGGDVSIEDLIADENVVITISH
                     AGYIKRTNLSEYKTQNRGGVGQKSAGTRDQDFLENMFVATNHQYMMFFTQKGKCYWMR
                     VYEIPEGSKTAKGRAIQNLINIESDDKVKAFICTQDLKDKDYINSHNLVMVTKQGQVK
                     KTSLEKYSKPRVNGVAAITIKEGDELLEAKLTNGESQIILAVKSGKLVRFEETKTRPM
                     GRTASGVRGISLKDESDEVIGMVTVDKEDITDTQILVVTENGYGKRTKLVDEDGEDVY
                     RITNRGGKGVKTLNITEKTGKLISINNVTDADDLMIINKSGLTIRMAVEDLRVMGRAT
                     QGVKLINLKGSDSIAAVTKVMKDDVAEVVVDEDGNVIETEAIERVKPVLEVLEDEGVE
                     DDDDDSDEEEIDDEAEDTEDEEQDDSDE"
     gene            16959..>17798
                     /locus_tag="PLQ70_02070"
     CDS             16959..>17798
                     /locus_tag="PLQ70_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33431.1"
                     /translation="MKSKNVILASALLISVVAFAQKDQIKAAEKALKGGNPQEAITIL
                     DGAQSLISNAVDTEKAQYYFVNGNAHLELANKKVEEGKNLTSAAKSFQELLAVEKASG
                     KSKYSSQATTSIIDIKGKLLNSAIADTKADRDIEGAQKLYDAYLLDKKDTINLYYAAS
                     TYVNAKDYPTALKMYSELKTLNYSGKGTSYLAVNKASGSEDVFNSVKERDLAVKLGTH
                     EKPKTEAIPSKRGEIYKNIALILVDAGKIEEAKKAISDARKANPEDGSLLLTEANLYL
                     ETKD"
BASE COUNT         5543 a         3040 c         3269 g         5946 t
ORIGIN      
        1 aagcaaaaat attgctttga gcagtgtggt ttatctcaac tcatttttaa caattgcgat
       61 gcgaaataaa attcaaaaaa tcattccatt tgttgcggtt attatcggaa taatgtttat
      121 ctttaggggg ttaggacttg gcattcctta tctatcccct tcaaacatga gtttgtttgt
      181 gcaatcaagt ccaaattgtc attaattatt aattaattct tatataatta tggaaaacca
      241 agatctgcta cacgtctctc ctgatgttca ggaaaaaatc gagaaattaa taactgagaa
      301 tgatcatttt gcagccttat tcgaagagta ccgtaaagta aagcaagaag tagcctcaat
      361 caaatcggaa gtagttgtaa ccactgatga gcatttgaaa gaattaaaag ttaaaatcct
      421 tcatttaaaa gacgaaatat attcaatctt aagaagataa aagcaaaaaa aatcacgctc
      481 tagggcgtga ttttttatga tatatatctt ttattaaatc gtcatcgaga ataatttagt
      541 atcaggtgct tttcgtttta aacgcaagtc aaaagccata caaatgttgc gcacaaaagg
      601 ttttccagca ttggtaactt taataccatt tttttcgatt aggactaatc catccttttc
      661 catttctttt atttgaatta gaatttcagg aacttcgtca aaatattggg ttttatcttt
      721 ccaagaagtt tcaaaatggc acattaaatt taaaatgtgt ctcctgatga ttaaatcttc
      781 tggggttaat aaatgacctc taaaaatagg caatttatcg gctttcaaaa gtttataata
      841 ctcttctaat tcttttacgt tttgagcaaa actgtaccaa ctatcgctaa tagaagaaac
      901 accaagtcca atcattagtt gggttttaga ggagctatag cccataaaat tacgatgcaa
      961 ttttttttgt tcgaaagatt catataacgt atcggttttc aaagcaaaat gatccatccc
     1021 tatttcgtag tattcattct cgaaaagcaa atctttaccc acttcgtaaa gcattcgttt
     1081 cacttcatct tttggaatat cttcgtcgtt aaaaccgcgt tgcccgtttc cttttatcca
     1141 cggaacgtga gcataactat aaaacgccaa tcgatcgggt tgcaaagaat tggttttttc
     1201 aatcgtatct attacatcgt caatttcctg aaaaggaagt ccaaaaataa tatcgtgacc
     1261 aatagaagtg taacctattt cctttgccca aaaagttact tttgccacat tatgaaaagg
     1321 ttggtagcga ttaatggctt tttgaacttt ttcggaataa tcttgaactc caaaactcac
     1381 tcttctgaaa cctaaatcat ataatttttg caaatgggca cgagtcgtat tattgggatg
     1441 tccttcaaag ctgaattcat gatctttggc tttttcggca tagcaaaaaa taccatttat
     1501 taaatcttct aagttttttg tagagaaaaa agttggcgtt cctccaccca aatgaatttc
     1561 ttttatgatg ggtttttctt ctagaatgtt gcaataaata gcccattcct ttagaatggc
     1621 ttcaatgtat ggatgttcga catcgtgatt ttttgtaatt cgtttattac aaccgcaaaa
     1681 agtacacata ctctcgcaga agggtaaatg aatgtacaaa ctaagacctt cttccgtatt
     1741 actttctgta aacgaccttt ttaaggtgtc aatccaattt tgataagtga aatcagactc
     1801 atcccaatat ggaactgttg gatagcttgt gtatcttggt ccaggtacat tgtatttttg
     1861 aaggattgag tttttcatag aaaatagtat tattcagtaa tcaaaattaa tgtgaaattg
     1921 ttaaaattaa aatgataatt atcatatttc attttgggta caaaaaaaac ctcattcaga
     1981 agataaagaa tgaggttttt cggatgctat taataggtgt taagattgat ttaaacttct
     2041 aacttgtctc agtttatcct tcaaaatctc catttcttct ttgtgttttt cgatgttttt
     2101 tcgaacttct aatacgatag aattttcttt tttagcattt cgagtgttgg taaaaaactg
     2161 aatattattt tccaattgaa aaatttcatt cttgatttca tcaattttgc gcattaagaa
     2221 aattttctct ccttcgagtt ttcgggtatc attgttttcc gataattgat ccattctatt
     2281 agagaaacgc atcatttcag aatctttttt actcaaactc aatttttcaa aaagagcatc
     2341 tagaacttta ttgaacttcc cttcaatgtg tcttcttgtg aaaggcactt ttccgaatcc
     2401 tttccaggtt tcgatatgta attttatggc gtctaaatcg gttttatgaa caccagtcaa
     2461 ctgaaattct ctcaaggttt ctagataggc tttctttttg tcaaaagctt ctatttcttc
     2521 gctgttttct tcgttttttt gctcttttag tttgtcaaaa taatgattgc aagcgtcttt
     2581 aaactcaatc caaattttat cagagtattt tctaggcacg tgacctattt gtttccactc
     2641 ttcttgaatt tgtttcatta ttggtgtagt tgtagcaaaa tcagtacttt cttgaagttc
     2701 tttagcttta gcaacaagag ctgtttttcg gtttaaattg ttgttttgat cttttttgat
     2761 gtctttgtaa aaagaatttt tgaaagaatt aaaatttctt acagctgttt taaaagttgc
     2821 ccaagtttcc tcattcaatt ctgcaggaac ttttccagcc gagaaaaaag cagttctcaa
     2881 agcttctact ttttctattt gctgcaacca ttgcgagtga gcatttactt tttcggtagc
     2941 caaaacttca attttggcaa taatttcttt tttcttttct aagttttcga gttcagttcc
     3001 tctcattttt tcaaacaaaa cttcacgttt atcgtgcatt tgcttggtta agtcgctaaa
     3061 tttattccaa atttcatcac gaaattctct tgaaacggga ccaatgtctt ccttccaaat
     3121 tttgtgtaaa tcttgtaatt ctctgaatgc tttgttgacg tctgcttcat taatcaattc
     3181 ttcaacacgg gctacaattt tttgtttttg ctctaaattg tgtttaaaat caaggtctcg
     3241 cgcttctctg tctaaatgca aatagtcata gaaattttca acatgaaaat gataattgtt
     3301 ccaaacgtgg ttgtatttat ctttcggaat ggatcctgca tttttccatc gttctctcaa
     3361 atcattaaaa tgtttcaagg tatctttgat gttttcctgc ggattgatga gttctttcaa
     3421 ttcttcgaca atggccaatc tgttttctaa gttggttttc aagttcgttt gcaaactttt
     3481 gaaatgggca tttttattgt ctctaaacaa cgaataatat tggtcaaatt gtgattttaa
     3541 aggcgaatga taggcaaact cctcagtagt atctgggttt tcagccagaa attcctcttt
     3601 tttctcttct aaaagatggt tgtatttggc taagaatgat ttttttattt cttcgacatg
     3661 atcctttacc gacatcactt tttcgttggt aaccaatttt ttcaattcgt caacaagtga
     3721 ttctagtgat agagcgtcat aatccagcat tggaatatcg tgacgttctt tcaaggtttc
     3781 gtcttcgctt tcttcagcat tattttcggc aatagcatcg ataatggttt ctgtttctga
     3841 tgcttttgct tcagctactt cagtcgattc ctcttcgata ggctttgcca tttcaattgt
     3901 atcttcaacc gaaacttctg gagttaaaac ggtttccttt tccacttctg caatagtttc
     3961 agaaacgatt tccgcatttt cttctaccaa aggagtttca atcgaatggt tttctaaatt
     4021 tccatctgct tcaagcaggt tatcattctt ttcttctaac attttttcaa tgtataaggg
     4081 ttcattattt tttcgaatgc gaaagatagt aaaggttgtt caaaattcaa aggaattcct
     4141 ttatttatag tggttttatg ttttaaaatt ggtttttaat ggttcaattc cgaagtttaa
     4201 aaatagaatt ttaaagtata attaatacca attcatagat tttacttctt ttaattgaac
     4261 gaaaacttca gtttgttagg aattttttaa ttaattggga gtcaatgttc ttttttaaaa
     4321 ctactttgtt tagctagcag tcatatctat ttatttttgt atttttatcc aacaaaatcg
     4381 attaaatgag accaatatta ccaattaaca aatccgaaat tctaaaaaga tacaaaccta
     4441 taaatcctaa tactcacaaa ggaattcaag gtcacgcatt gctaattggc ggaagctatg
     4501 gaaagattgg agccataagt cttgcttcta aagcttgttt gaaaacaggt tgtggcttgg
     4561 tttcggtttt tgtgcctaga tgtggttaca aaattatcca aactgcagtt cccgaagtaa
     4621 tggttttgaa cgatgtctcc gtaaaatata ttactcgaat tgttttcgat tttgtgccca
     4681 atgctatcgg aattggacct ggaatggggc aggatatcga aacgcatacc gccttgcatc
     4741 gatttgtgaa aaccaataaa gtccctttag ttattgatgc cgatggtttg aatattttgt
     4801 ctcaaaacaa acaatggctt acttttttgc cagagaaaac gatttttact ccccatcaaa
     4861 aagaacttga acgtctcatt ggacaatggg attcttttgc tgataaaata gagaaaacca
     4921 ttgcattttc aaaacagaat aaccttatta tggtaatgaa aggtgctcca acttttatta
     4981 tcgatggaga taccatttac gaaaacacta ccggaaacgc cgcattggct accgcaggaa
     5041 ctggcgatgt gctaaccgga atgattacga gtttgttagc acaatcctat gacccaattg
     5101 atgctgcaat tctaggcgtt tacctccacg gattgactgc tgatattgcc ttgcccgaaa
     5161 cgggttatca gtcctttatc gcttctgatt gtattgcgaa tattggaaaa gcctacttga
     5221 gtttggaaaa aatagagtag taattagaga aaagaaaata gataaaagac aaaaaaacaa
     5281 agatttaatt cgattaaatg tatcgttgaa tattgctaaa acttaatatc ttttttttct
     5341 tactttgctg aaaaaaaatc accttatgaa ttttatctcc aatcgaacta taattataaa
     5401 gtcgcttata gcagccattg ctattttggg aatcaaaaat tttgttttca aaaacctttc
     5461 tttatacgga caggattttc acgatttgat cgaactgtct gatataacta ttattttcac
     5521 tggagctttt ttcgtgtttg gattgctttt agcggcaacc atgaccgatt ttaaagagag
     5581 tgaaaaaatt ccaggcgaag ttgcttcaaa tctagaagct atcaaagact ggatttattt
     5641 agcttttaag gcaccaagga caggaacaac tgaactgtgt aaggaagaat tgaataccgc
     5701 ctttttgcgt caagaaatgg ttgatattac agaaggaatc attgcctgga tttattctag
     5761 tgaaaaagag tcaaatgtta tttttccatt aattcgaaaa tccaacgaaa tcgcctatta
     5821 ttttgccgaa agaggcgttg acaaagaagc cataaaaggc attcaagaaa acacaaacgc
     5881 catgcgaaaa cagcttacta gagcctattc tatttcaaga aacaacttca tcaagcctgc
     5941 ttacactttg ctgcaaagca tcttgtttat tgtaatgtcg cttttgttga ttaccaaatt
     6001 caagtcgcct tcagccgatt atttggtgac ttcggccatt acgtttttgt tttgctattt
     6061 gtatttattg attagcggtc tggacgatcc ttttgatgtt ttcaatggcg ataccaatgt
     6121 cgatttaaaa cccattgacc gattcaaaca acgattgttg tctgattttt tggtttaaaa
     6181 taaggggtgt tgttccagtt tgatgacatg aatttttact gacaaacggt ttgtggtttg
     6241 gcaaagaagc gggtttgtaa aagtataatt tattgatttt tatacttatt ttgatcccga
     6301 agcgtcggga tagtttttga atgaggttgt gcaacagcta cgctagttgg gcgaaatgcg
     6361 catttcactc caactcataa atatgcgaaa caaattttca cagtgctcca tataatctaa
     6421 gttgattctc tttacaattt atctcaaata aattattttt ttttcttaca aatcaatgat
     6481 tacctttttt attcgttgta atttcttata ttttattcaa aaacttcaac tcccataaac
     6541 ttttaaaagt taatagaatc ctaccgtttt ccctcaacaa aattccttaa atttatgacc
     6601 taaaattcaa tgtcaaaaat gacaactacc tatactatta ctggaatgtc ctgcaacggc
     6661 tgccgcacca aagtcgaaaa agccttgaac gaaatagacg gaatggaagc cacagttact
     6721 ttagatccgc cagtagcaac gattgtttcg gagaaaccaa tatccgtttt caaacttcaa
     6781 aaagcggttt acgtagtggg aaattatatc attagtgaaa gcaacgcttc tcacgaaaat
     6841 tcgctttaca gcacacccat tccaagcgaa atgaagcctg ttgacttgcc tcaaagtgct
     6901 tctggaaaat attattgccc aatgttttgc gaaggtgaca aagtctatga cgaacaagta
     6961 ggttgccctg tttgcggaat ggatttggtc aaagctcccg aattaatggc cgccaaaaca
     7021 caatatactt gcccgatgca tcccgaaatg gttcaagatc aacccggaaa ttgttctatt
     7081 tgcggaatgg atttggtcgc tatacaagct gccgaagaag aagattctgc ttatcttgat
     7141 ttgtggcgca aaatgaaaat ctcattaatc ttcaccattc ccgttttcat tatttcgatg
     7201 ctggaaatgc tacacaacaa tccgttattg gaattgatgg aaacccaaaa atggaattgg
     7261 gtacaattta ttttggcact tcccgtagtt ttttatgccg gttggatgtt ctttgttcga
     7321 gcgtggaaat ccatcctgac ttggaatctc aatatgttta cactcatcgg aattggaact
     7381 ggagttgctt ttttgtttag cgtagctgga ttgctattcc cccaattttt ccccgaagaa
     7441 ttcaaacatc gcggcacggt ttcgctgtat tttgaagcca cagtcgtgat attaaccttg
     7501 gttttattag gacaattaat ggaagccaaa gcccacggac aaacgaataa agccatcaaa
     7561 gaattgttga aactcgctcc aacccaagct actttggtta aaaatggaga ggacaaagtc
     7621 atttccattc acgatatcaa aaaaggagat gtattgcgag taaaacccgg agaaaaaatt
     7681 cccgttgacg gaaaaataac tgagggcgaa agtactattg acgaatcaat gatttcagga
     7741 gaacccattc ctgttgataa aaccattgaa gatgcggtta tggctggaac catcaacggc
     7801 aacaaatctt tcgtgatgct agccgaaaaa gtaggttcgg aaactttact ttcgcaaatt
     7861 gtacaaatgg tcaatgatgc cagtcgttcg cgagcaccca ttcaaaaatt ggcagatagt
     7921 attgccaaat attttgtacc aactgtggtg ggaattgcca tactaacgtt cattatttgg
     7981 gcaaattttg gtcctgaacc tgcaatggtt tacggtttta ttaatgcagt tgcggttttg
     8041 attatcgcct gtccgtgtgc tttgggattg gcaacgccta tgtcggttat ggttggagta
     8101 ggcaaaggag ctcaatcggg agttttaata aaaaatgcag aagctttgga gaatatgaac
     8161 caagtaaacg tcctaattac cgacaaaacg ggaacgatta cagaaggaaa accatcggta
     8221 gaaagagtat tttcttcgaa taatgaagaa gataatgtgt tgcaaaacat cgcttccttg
     8281 aaccaataca gcgaacatcc tttggcgcaa gccgttgtga attatgccaa aacaaaaaac
     8341 atttcgttga tggaagtcaa agatttcgaa gccatttctg gcaaaggcgt tatcggtacg
     8401 gttgctaata aaaaaatcgc tttaggaaat aaaaagttga tggaacaagt aaacgctagt
     8461 gtttctgacg atttagaaag caaaattatt gccgagcaaa agttaggcaa aaccgtttcc
     8521 tatgtttccg ttgatggaat tgctgttggt tttgtatcca tttccgataa aataaaaact
     8581 acgagcgccg atgccattaa ggaattgatt cgtcaaggtg tggaagtcat tatgctgacg
     8641 ggcgataatg aaaacacggc aaaatcagtc gccgaacaat tgcatttgac tcgttttatt
     8701 gccaattgtt tgcccgaaga caaactcaac gaaatcaaac gtctgcaagc cgaaggaaaa
     8761 atagtagcaa tggcaggcga cggaatcaac gatgctccag cattggcgca agccaacatc
     8821 ggaattgcaa tgggaacagg aaccgatgtg gcgatagaaa gtgccaaaat cacgttggta
     8881 aaaggcgatt tgcaaggcat tgtaaaagcc aaaaaactaa gtcaggcagt aatgaaaaac
     8941 atcaaacaga atttgttttt tgcgtttatc tacaatgtat tggggatttc ggtggcttct
     9001 ggaatgttgt atcccgtttt tgggttgttg ctttctccaa tgattgctgc ggctgcgatg
     9061 agttttagct cggtttcggt aatcttgaat tctttaagat tgaggagttt taaaatgtaa
     9121 aaaaaaagat tgtagatttt tgattaacga tttttgattg cagatttcaa gtccctaaaa
     9181 aatgtatttt atttactaac acaaaatctg aaatcaaaaa tcgttaatct gcattcaaaa
     9241 atcacttaat ttaactaacc catcgttttt ttacttcgtt tgagtctttt gtaatgtgtt
     9301 tgacgtagat tgttttggtc agaaatgata ctgatattgc catcaatttc tagcattgct
     9361 agttttacat ccgaaaaatg ttcgactccg tgttcgcgaa tagcttcttg tagttcttcg
     9421 gaagtgatct ttaatttgct caaagtcttg aaatctaatt tgccttcgtg gattaagatt
     9481 tctggtttgt cttgcataaa atcactgaat ttgggaaact taaatattag tcttttaaaa
     9541 gtaaaattga tgataaaaag tactgctgcc gcagctaaac ctcccagaag actggtgtcg
     9601 cttcctacca tcgcattttg aaccgagtta ctaattaaca atatcaaaat aacatcggca
     9661 gtgttgagtt gtgataattc ctttttgccg aaaatccgca aagcgatgag cataaataaa
     9721 tatacggaag cgctacggag gatgatgtct aaatagggat tcattgtttt ttttctttaa
     9781 aaggtttcaa gtttcttgaa ttcggctaac tgactactgc aactgaccac taatttagtt
     9841 taaaattctt ttcaattgcc agaatcatct cgcctgcaat gtctttgttg gtagcacttt
     9901 cgatgccttc gagtcccgga gaggaattca cttccagcaa caaaggccct ttagaggaac
     9961 gaataatatc gacgccagcg actttcaaat ccatagcttt ggcagcacga atggctattt
    10021 ttttctcttc ggcagtgggt ttgatgactg atgccgttcc gccaagatga atattagctc
    10081 ggaattcgcc tgctaaagct tctcgttgaa tggcggctac aactttgcca tcaattacga
    10141 ataatcgaag gtcttttccg ttggcttctt tgatgaattc ttgcaccaaa atattggcat
    10201 ttaaactctt gaacgcatta ataacgcttt ctgctgcttt tttggtttcg gcaagaacga
    10261 cgccttttcc ttgagttcct tccaataatt tgacgattaa aggagttcct cccaccattt
    10321 ttatcaaatc gtcggtgtct aaaggcgaat gggcaaaacc cgttgtgggt atttcgatac
    10381 cgctttgcaa gagcaattgc agtgaaaata atttatctcg agattgtgtg atcgcagtcg
    10441 atgaattcaa acaaaaaaca ttcatcgctt cgaattgtcg agtcaaagca caaccataaa
    10501 aagtaatact tggtcgaatt cttggaatta cagcatcaaa ttgattcaaa attaatccgc
    10561 ctcgataatg gatttcggga gttttggcat ccagtttcat atagcattcc ttgatgttca
    10621 gaaagtgcat ttcgtgacct ctcatttcgc ccgcttccat aatgcgtttg ttgctataca
    10681 attcgggatt gctggctaaa agtgcgattc gaatgccagt actggctttt ttagcatttt
    10741 tatagacttc tttcaaactt tcgcttgtag gttcgcctaa aagatattgt tgttcgggat
    10801 ctaccatcac tctgccactc atcgcttcac gacccagaag catacgaaaa cccatcgaat
    10861 ctcgattggt caaagtcatt tcgataaccc atttggaatt accaatttct aaattggttt
    10921 gaatgacata gcgttcttct cgaaaaccgc tagaactttt tacaattctt ttatctacca
    10981 aaggagcttc gcaatgaatc accgttttta cattgttttg gatgggatta atatcaaatt
    11041 tgacccagtt ttggctttct tttttgaagg gagcaatatt aatggcgtgc aaagccgaag
    11101 tttttgcgcc agaatcgacg cgtgctttga tggtaggaat tcctaattca ggtaaggagc
    11161 accattcttc gctgcccaat ataattttgc tttgtaacat aaatgagttt ggtggtatta
    11221 ggttgtaaat atatagggat aaaacaactt tggatgttaa attacggtta aatcaggcaa
    11281 tcgaacagac ctaaatgtag taaaaaaaag agccacgaat tcattaattt cacacattaa
    11341 ttagtgaaat tagtgaattc gtggctaaaa aagttctaaa aaatggaatt aattagttgg
    11401 ttcttctgct ttttgaattt taacagataa ctcttgtgaa gtttcgtctt ctaaatccat
    11461 aaaaatttcg tctccagctc ctatttttga cgtaatgatt tcttcggcga gagcatcttc
    11521 aacatatttt tggatggctc ttttcagtgg tctagcacca aattgtctgt caaaaccttt
    11581 gtcggcaata aaagcttttg ctttgtcaga aagaattaaa ttgtagccca aatctttgat
    11641 acgagcgtat aattttttca attcaatatc tataatcaaa ttgatgtctt ctttttctag
    11701 agcgttaaac acaattacat cgtcaattct gttcaagaat tcaggagcaa aagttttttt
    11761 caaagcattt tcgataatac ttttcgagtt atcatcggct tgggctactt tggcagcagt
    11821 tccgaaacca acaccttgac caaaatcctt caattgtctt gctccaacat tcgaagtcat
    11881 gataattata gtgtttttaa agtcaatttt tcgtcccaaa ctatctgtta aatagccatc
    11941 gtcaagaact tgaagcatca tattgaaaac atcaggatgt gctttttcaa tttcgtccaa
    12001 aagcactacg caatacggtt tgcgacgtac tttttcagtc aattgtccac cttcttcata
    12061 acccacatat cctggaggcg ctcctattaa tcttgaaatg gcgaattttt ccatatattc
    12121 actcatatca attcggatta aagcatcttc agaatcgaat aattctctgg ctaaaacttt
    12181 ggctaattgt gtttttccaa ctccagtttg tcccaagaaa atgaaggaac caataggtcg
    12241 atttgggtct ttcaatccag cacgatttct ttgaatggca cgagcaattt tctttacagc
    12301 ttcgttttgt ccaatgactt tgtttcctat aagttctggc aaaatagcta gtttattact
    12361 ttcggtttga gcaattcgat ttacaggaat gcccgtcatc attgaaacta cgtcggcaac
    12421 attatcttca gtaacttgaa ttcgattgtt tttggaatct tcttcccatt gttcttgggc
    12481 gattgccaaa tctttttcaa ggcgtttttc atcatcacga agtttagctg cttcttcata
    12541 tttttgtttt ttgacaacag catttttcaa ttcacgaaca tcttctaatt gtttttctaa
    12601 atccaaaacc tgtgcaggaa cttcaatatt tatgatgtga actctagatc ctacttcgtc
    12661 catagcatca atggctttgt ctggtaagaa acgttccgac atatagcgat cggttaattt
    12721 tacacaagct tctatagctt ctgggctata agttacattg tgatggtctt catatttgtc
    12781 tttgatattg tttagaattg taatggtttc ttcaaccgaa gttggttcaa taattacttt
    12841 ctgaaaacgt ctttctaaag cgccatcttt ttcgatatat tgacggtatt cgtctaatgt
    12901 tgttgcgcca atacattgaa tttctcctct tgctaaagcg ggtttaaaca tattggaagc
    12961 atcaagcgag cctgttgctc caccagctcc aacaatagtg tgtatttcgt caataaaaag
    13021 gatgatgtca tcatttttct ccaattcgtt tatcacggct ttcatacgtt cttcgaattg
    13081 tccacggtat tttgtgcctg caacaaggct ggccaaatcc aatgttacta ctcgtttgtt
    13141 gaataagata cgtgatactt tcttttggat aatacgtaaa gccaatcctt cggcaatggc
    13201 agattttcct actccaggtt ctccaattag tagtggatta ttctttttgc gacggctcaa
    13261 aatttgagaa acacgttcta tttctttttc acgtcctaca actgggtcta attttccttc
    13321 ttcggccatt tcggttaaat ctctcccaaa gttatctaaa acaggagttt ttgatttttt
    13381 gttcgatttg ttggctggat tgttgaaatt cccttctttt agactgtcat cttgtcctga
    13441 atcttcgtta taggcgtcat ttttgggtaa attctcgata aaattttctt cagatggtgt
    13501 catatttaga tattgttctt tggctacatc ataatcaata ttgagtttat tcagtagctt
    13561 ggttgtggga tcattttcat ttcttaagat gcacaataat aaatgtgctg tactaattga
    13621 ggtgctgtga aagacttttg cttctagaaa agtagttttc aatgcacgct ctgcctgtct
    13681 agttaggtga agattctttt tttcgttgtt gctatccatc gtgggactcg ctggacttag
    13741 aacttccact tttcttcgca agaggtttaa atctattgat aaattattca atatattaat
    13801 agctttgccg tttccgtctc tcaaaatccc aagcataaga tgttcggtac caatgaaatc
    13861 gtgacctaaa cgcaaggctt cttctttgct gtaagtaata acgtctttta ctctaggtga
    13921 aaaattatca tccatattgt cttgtatttc taattgtaaa attagtgaat tggtgtttga
    13981 aaaacaaaaa tcattcctat tatgaggttg tgtcagctaa ttgacaaaaa aatcaacaaa
    14041 taatcgttaa aacttacata atttattaac taaaaaacaa gtttattttg ttaataaatc
    14101 attgaaataa gtacagaata gtttgtataa aaacattgga atgatgtata ttagcacgtt
    14161 ttgaaattaa actaaattta ttaaatatta caacttatgt ctgaaggaga aaagttaatt
    14221 cctattaaca ttgaagatga aatgaaatca gcttacatcg attattcgat gtcggtaatt
    14281 gtgtcaagag cgcttccgga tgttagagat ggcttgaaac cagtacaccg tagagtactt
    14341 tatggaatgt atgatttggg agttaattca agatctgccc acaaaaaatc ggcgagaata
    14401 gtcggagaag ttttaggaaa gtatcaccct cacggagata cctcggttta tgatgccatg
    14461 gttcgtatgg ctcaggaatg gagtatgcgt tatttattgg tggacggtca aggtaacttt
    14521 ggttcagtag atggtgatag tccagctgca atgcgttata ctgaggctag aatgaaaaag
    14581 atttcggaag aaattatggc cgatatcgaa aaagaaacag ttgattttca actaaacttt
    14641 gacgatactt tgtatgaacc aaaagtaatg ccaacaagag ttcctacctt attaataaac
    14701 ggagcaacag gaattgccgt tggtatggcg actaatatgc ctcctcacaa tttaactgaa
    14761 gtgatcaacg gaacgcttgc gtaccttgat aataacgata ttgaaataga cgaattgatg
    14821 aatcatatca aagctccaga ttttccaact ggaggtgtga tatatggtta tgaaggtgtt
    14881 cgcgaagctt ttaaaacagg tcgtggacgt atcgttatgc gtgctaaagt tggttttgaa
    14941 gaagtagatg gtagagagtg tatcattgta actgaaattc cataccaagt caataaagcc
    15001 gatatgatta aacgtaccgc tgatttggtt aacgagaaaa agatagaagg tatttctaat
    15061 attcgagatg aatcggatag aaatggtatg cgaatcgttt atattttaaa acgggatgct
    15121 acgccaaacg tggttttaaa tacactttat aaattcactc aattacaatc ttctttcagt
    15181 gtaaataaca ttgcattagt aaaaggtcgc ccacaaatgt tgaatctaaa agatatgatt
    15241 cactatttta ttgagcaccg tcacgatgta gtagttcgta gaacccaatt tgaattgcgt
    15301 aaagccgagg aaagagcgca tattttagaa ggtttgatta tcgcttctga taatattgat
    15361 gaggtaattg ctttgataag aggttcgaaa aatactgaag aagctcgtga aaaattaatc
    15421 gaaagattcc aattgtcgga tattcaagca cgtgctattg ttgaaatgcg tttgcgtcag
    15481 ttaacaggtc tagaacaaga caagttaaga gcagagtttg aagaattgat gaaattaatt
    15541 gagcatttga aagcattatt agcagatgtt gatttaagaa cggctttaat caaagaagaa
    15601 ttagaagaaa ttcgtgagaa atatggtgac gctcgtcgtt cgcaaattga gtattcaggt
    15661 ggagatgtta gtatcgagga tttaattgcc gatgagaatg tggtaatcac catttcgcat
    15721 gcaggttata tcaaacgtac taatttatcg gaatacaaaa ctcaaaatag aggtggagtt
    15781 ggacaaaaaa gtgcaggaac cagagatcag gatttccttg aaaatatgtt tgtagctacc
    15841 aaccatcaat atatgatgtt ctttacccaa aaaggaaaat gttactggat gcgtgtttat
    15901 gaaattcccg aaggtagtaa aacggccaaa ggaagagcga ttcaaaactt aataaacatc
    15961 gaaagtgatg ataaagtaaa agcattcatt tgtacacaag atttgaaaga caaagactat
    16021 atcaatagtc ataatttggt tatggttact aaacaaggtc aagtgaagaa aacttcttta
    16081 gagaaatatt caaaaccaag ggtgaacgga gttgcggcaa ttacaatcaa agaaggagat
    16141 gaattattag aagctaaatt aaccaacgga gaaagtcaga ttattttggc tgtcaaatcg
    16201 ggtaaattag ttcgttttga agagaccaaa acacgtccga tgggaagaac agcttctggg
    16261 gttcgtggaa tttccttaaa agatgaatcc gacgaagtaa ttggtatggt tactgttgat
    16321 aaagaggata taactgatac tcaaatttta gttgtaactg aaaatggata cggaaaacgt
    16381 actaaattag ttgacgaaga tggcgaagat gtttatagaa ttaccaatcg tggtggaaaa
    16441 ggagtgaaaa ctttgaacat tactgaaaaa actggaaaac taatttcgat aaataatgta
    16501 accgatgctg atgatttgat gattatcaac aaatcgggat tgactatcag aatggctgtt
    16561 gaggatttga gagttatggg tcgagctacg caaggggtta agttaattaa tctaaaagga
    16621 tctgattcta tcgctgctgt aactaaagta atgaaagatg atgtagctga agtggtagtt
    16681 gacgaggatg gaaatgttat tgaaactgaa gctatcgaaa gagtgaaacc tgttcttgaa
    16741 gttcttgaag atgaaggcgt agaggacgac gacgacgatt cagatgaaga agaaattgat
    16801 gatgaagccg aagatacaga agatgaagag caagacgatt cagatgaata gaggtgaatc
    16861 ataaataaac taaaacattt atcagatatg atttttatca taattgataa atgtttttct
    16921 aactactttt gcaagtaaat taaattaaat caaaaattat gaaaagtaaa aatgtaatac
    16981 tagcgtcagc attattgata tcagttgttg cttttgctca aaaagatcaa attaaagctg
    17041 ctgaaaaagc gttaaaagga ggtaatccac aagaggctat aacaatttta gatggtgctc
    17101 agtctttaat atccaatgct gttgatactg aaaaagcaca gtattatttt gtaaatggga
    17161 atgctcattt agagttggcc aataaaaaag tggaagaagg gaaaaattta acttccgcag
    17221 ccaaatcatt ccaagaattg ttagcagtag aaaaagcttc tggaaaatct aaatattctt
    17281 ctcaagcaac cacttctatt attgatatca aaggtaaatt attgaatagt gccattgctg
    17341 atacaaaagc agatagagat attgaaggtg ctcaaaaatt atacgatgct tatttattag
    17401 ataaaaaaga caccatcaat ttgtactatg cagcctcaac ttatgtaaat gcaaaagatt
    17461 atccaacagc tctaaaaatg tattcagagt tgaaaacttt aaattattca ggtaaaggga
    17521 ctagctattt ggctgttaat aaagcttctg gtagtgagga tgtgttcaat tctgttaaag
    17581 agagagattt ggcagttaaa ctaggcactc acgaaaaacc aaaaacagaa gctattcctt
    17641 caaaaagagg cgaaatctat aaaaatattg ctttaatatt agtagatgca ggaaaaattg
    17701 aagaggctaa aaaagcaatt tcagacgcta gaaaagcaaa tccagaagac ggttcattgc
    17761 ttttgactga agctaatttg tatttagaaa caaaagat
//
LOCUS       DAOSNN010000020        17709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3223_length_17709_cov_5.542427, whole genome shotgun sequence.
ACCESSION   DAOSNN010000020 DAOSNN010000000
VERSION     DAOSNN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17709
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3223_length_17709_cov_5.542427"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="PLQ70_02075"
     CDS             <1..1432
                     /locus_tag="PLQ70_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33432.1"
                     /translation="VKYHFQKNWVGTSLKLEDNQEKDKVNNQFSALSQRFTEYGLFAG
                     RGDSTKVFVELGYFKRVNDSLQNGSIQRVNNSQTLALRSKLIQTSKSDLSLFVNYRVL
                     NFVDATRKNEPSLNSRMMYNDRFFNQLVQWTTVFETNSGSIPQQEFTYLEVPVGQGVY
                     TWNDYNSNGIQELEEFEVAPFPDQAKYIRVYLPNRIYIKTHQNKFSQSLILNPNQWQN
                     EAGFKKIVSYFYNQTSYLVDRKIENKGDNFDLNPFSKSEENVLGLSSSFRNSLFFNRG
                     KQWHSVTYTYVQNENKNLLSIGSQEAKNSSNQLQYNHLYNKSWLFGFFTKTIQTSLTS
                     ENYPDKNYEIKGYQLAPKISYLFSKSTSWDVFYELQNKENQIGNLETLLQNRFGTSFT
                     YAGKKNFTMNGEISFYQNKFVGNDLSSVGFQMLEGLQAGQNLTWRLLFQKNLTNFLDI
                     NFNYQGRKSETSQTIHTGSVQLRAYF"
     gene            1510..2043
                     /locus_tag="PLQ70_02080"
     CDS             1510..2043
                     /locus_tag="PLQ70_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33433.1"
                     /translation="MKKLILLCFLFVFSNTFYAQTTKSKSEPTSKETTKKKSSYNYDK
                     DVPVKSTKKEADSKTKSTKEKTKKEADSKVKSTKEATKKSGYDYDKDVSVKSKSNNAT
                     KTNKSDVNKIDAKIKDEKYTKGNSNKKTTYKETNEKAPKTPDRVTGEYKGKKVYTGSR
                     GGKYYINKNGNKTYIQD"
     gene            2187..3251
                     /locus_tag="PLQ70_02085"
     CDS             2187..3251
                     /locus_tag="PLQ70_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE33434.1"
                     /translation="MSFNTANKTILIAPLNWGLGHATRCIPIIKALQENNFIPIIASD
                     GIALELLQKEFPYVQTLKLPSYQIEYAKNGKNFKWKLLKNFPEMIKAIWKEEKKVRKW
                     IKKYDIDGIISDNRLGVFSKKIPSVYLTHQLNVMTGSTTWFTSILHQHIIKKHDECWV
                     PDFEGTSNLSYKLGHIENPDFNLKYIGALSRMQKKETPKLYDLMIILSGPEPQRELLE
                     DKLKVEILKYKGSVVFIKGIVEKDQKKEQIDHVTFYNFMNSRQLEQTFNESEIVLCRS
                     GYTTIMDLVKLEKKAYFIPTPGQYEQEYLAKKLKEEGLVPYSKQDNFSIEDLKEIENY
                     KGLPLLEIPINWKHLFEVFE"
     gene            complement(3286..4308)
                     /locus_tag="PLQ70_02090"
     CDS             complement(3286..4308)
                     /locus_tag="PLQ70_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQE33435.1"
                     /translation="MKAKATLKQIAKELNVSVSTVSKALNDSPEISEQTKTRIKEYAK
                     LKNYKPNVIGLNLKNRKTKTIGVIIPNILNSFFAKVFTGIEKVAEEKGYNVITCISNE
                     SLKKEISTMDMLSNGTIDGFILSPSEEAQKLHEFNHFKEIISEGTPIVMFDRVVDEVE
                     CDKVVVDDFDSALNATQYLIDIGCRNIALLSSADNLSVGKLRANGYLKALQNNNIKVN
                     PDLIIRTDSEEDLKEKAEILFDNKSIDGVFALDENDSVAALRISLKKGFKIPENLSII
                     GFADGILASRRLSPSLTTVSQHGPEIGEAAAELLIERLEKGENEAPYKTTVIKTELRF
                     RESTRT"
     gene            4780..5235
                     /gene="rplM"
                     /locus_tag="PLQ70_02095"
     CDS             4780..5235
                     /gene="rplM"
                     /locus_tag="PLQ70_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQE33436.1"
                     /translation="MNALSYKTVSTSKATASKEWIVVDADGHNLGRLATKVAMILRGK
                     YKPSYTPHVDCGDNVIVINSEKINLTGNKLDEKTYVRHTGYPGGQRTLTAKVLQAKNP
                     ALLVEKAVKGMLPKNKLGAELFRNLNVVVGPEHKQGAQKPRTVNLNDLK"
     gene            5235..5621
                     /gene="rpsI"
                     /locus_tag="PLQ70_02100"
     CDS             5235..5621
                     /gene="rpsI"
                     /locus_tag="PLQ70_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQE33437.1"
                     /translation="MGVIHKIGRRKTAVARVYVSEGTGVITVNKKPFATYFPTATLQY
                     KVLQPMSMTENATNFDVKVNVYGGGSTGQAEAVRMALARVMCEVGEGNRAILKPEGLL
                     TRDPRMVERKKFGQKKARKRFQFSKR"
     gene            5838..6620
                     /gene="rpsB"
                     /locus_tag="PLQ70_02105"
     CDS             5838..6620
                     /gene="rpsB"
                     /locus_tag="PLQ70_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQE33438.1"
                     /translation="MSNKVEVKELLEAGVHFGHMTRKWDPNMAPYIYMERNGIHIINL
                     YKTAAKIEEANEALKKIAASGRKILFVATKKQAKDIVAEKAAACNMPYITERWPGGML
                     TNFVTIRKAVKKMSSIDKMKKDGTFNTLSKKERLQVDRLRAKLEKNLGSIADMSRLPA
                     ALFVVDIKAEHIAIKEAQKLNIPVFAMVDTNSDPREVDYVIPANDDASKSIDKILSIV
                     TAAVVDGLSNRGTEADIQPEAATEEVDAEVATAEVEAPTTEE"
     gene            6757..7719
                     /gene="tsf"
                     /locus_tag="PLQ70_02110"
     CDS             6757..7719
                     /gene="tsf"
                     /locus_tag="PLQ70_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQE33439.1"
                     /translation="MATITAADVNKLRQTTGAGMMDCKKALVEADGDFDKAIQNLREK
                     GQKVAANRSDRESSEGAAVSFINADKTKGAIITLNCETDFVGKNEAFVTLAKELVEKA
                     INFSSKEEFLASDFNGITVAEKLIEQTGVIGEKIEIGGFEILEGAFVGSYVHVNKIAA
                     LTAISAPIANGDVLTKDISMQVASMGADTLSYKDFDPAFVESELAARIAIIEKENEEA
                     ARLGKTLKNVPKYISFSQLTPEVIKQAEEDAKAELKAEGKPEQIWDKIIPGKVQRFIS
                     DNTTLDQEKALLDQNFIKDDSKKVGDYVKGFNVEITGFKRVTLG"
     gene            7899..8582
                     /locus_tag="PLQ70_02115"
     CDS             7899..8582
                     /locus_tag="PLQ70_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33440.1"
                     /translation="MAHFKKTLLFLIIFSLISCGKNKESQEHELEQKKEPSYEQTIET
                     INAEVPRKDTTKTSKEDSIIAIKHSILREKKYTKKKANTPTEEKKVTETKTVTENTSE
                     TNTTKPEIKKVKAPSFVYIKKILNECETGVTMTQEDLEAKFKIPKEAMKLVKSVTKTA
                     PDELDIKWKSTWLVEKISDAKFKDGKLKMRFEDNKMYTSGGAISIKYEKKLYSDLILI
                     GRAAYIPLQ"
     gene            8623..9183
                     /locus_tag="PLQ70_02120"
     CDS             8623..9183
                     /locus_tag="PLQ70_02120"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQE33441.1"
                     /translation="MELQRCSWCLSSDLYKKYHDEEWGVPVYEDQKLFEFLLLETFQA
                     GLSWITILNKRENFRKAFDDFDYIKIANYSEAKIQELLLDAGIIRNKLKVYSAVSNAQ
                     AFIKIQEEFGSFSKYIWGFVDGKPIDNHRKSLKEVQAATPLSDTISKDLKKRGFKFVG
                     STVVYAHMQATGMVNDHVEDCWTRNQ"
     gene            complement(9227..10252)
                     /locus_tag="PLQ70_02125"
     CDS             complement(9227..10252)
                     /locus_tag="PLQ70_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQE33442.1"
                     /translation="MKETTLKEIAETLGISITTVSKALKNYSDVSEKTRKAVYALAEE
                     LNYTPNSFAVNLRTKESKTIGLIIPEVVHHFFSNVINGIIAEAEKNGYLVIILQSNES
                     LELEKKQVALLINKRVDGIIMSLSNESNNDFHIKEILRKEIPFVQFDKISKLIPSSKV
                     IINDQKAAMEAVQHLIDKGCKKIAHIRGLENPQNSIDRFIGYKKALEKNGIPFDPKLV
                     YACKSITFEQGVEFAKQIIEDDLGIDGIFVITDLVAIGVLAHFNEIGIQVPGQIAIIG
                     FSNWFVSQVISPKLSTVDQPNYEMGVAAFGLLLEEIVCRKNGKPFEPKIVELGTSVIV
                     RESTLKK"
     gene            10407..12827
                     /locus_tag="PLQ70_02130"
     CDS             10407..12827
                     /locus_tag="PLQ70_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE33443.1"
                     /translation="MKKNHSSFKKIGLIIALLLFNLGFSQNETNPVVDTTKTTKLEDI
                     VITGVANPKAKIKSSVSITTMDVKQVEQSAPRSTAEIFRSIPGIRSESSGGEGNSNIS
                     VRGVPISSGGSKYLQIQEDGLPVLLFGDIAFATADIFTRFDGNTAKIEAIRGGSASTL
                     SSNSPGGIINFISKTGKTEGGMVRTSFGLDYNNFRTDVDYGTKIGEGLYFHAGGFYRA
                     GEGVRKTGVTSNNGGQVKFNLTKEFENGKITVYAKFLNDRAAAYMPMPVKVTGTNSNP
                     SWGSVSGYDATTGTLQSIYLNHNVGLGPDGQLRRESVADGMNPVSKSIGVNASFDLEG
                     GWKITENGRYSSNSGGFIAPFPAEVGSAAAIAASFGAGSKLTYADSGTPFNNPNGLVS
                     RIHMFDTQLNDLSNFMNDLRITKKFDKIGITAGYFKSLQNISTSWLWNSYLQEVSDNN
                     PRLINVTNATGTLLSDNGLYAYGTPAWGNLARNYDTQYNVSAPYVNLSFDATENLSFE
                     GGLRYDLGKVSGSFAGGTSRAYDINNNGTISVPEQNVFAVNTANETAVNYDYDYVSYT
                     LGANYLLTAKQSLFARYSKGAAAKADRILFSGLDYLDGSKINALDFLTQAELGYKLKF
                     PKGYAYATAFHSKTDEQAGYEASTNSFIKNNYKSYGLEIESSYNVMDNFNLKGAFTYT
                     KAEITSGDNDGNEPRRQPKLMYNLMPTYNFSSNKNTVGLSFIGQTKAYAQDSNQLVMN
                     GFVIVNAFVEVGITKGLSVNLSGNNLFDTLAITESEEGNITDNTVNYVRARPMPGRSL
                     SMALSYKF"
     gene            12921..14234
                     /locus_tag="PLQ70_02135"
     CDS             12921..14234
                     /locus_tag="PLQ70_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007095039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE33444.1"
                     /translation="MAFSKPKLNFWQILSMNVGFFGIQYSFGLQQSAVNPIYDFLGAS
                     PDQIPLLNLAGPVTGLLVQPLIGALSDKTWHPKWGRRKPFFLIGALLCSVALLLFPFS
                     SSLWMAAGLLWILDAGNNTAMEPYRAFIADKLDEEQQPLGFQMQSFFTGLGQTLANLS
                     LFIFPMIIIGKTGSLPTWVYASFFLGAICSITTIWWSIKTTPEIPPTEEELAAIRAKK
                     GGLFEPLIEIIDAIKEMPKVMWQLALVYLFQWYALFCYWQNSSKSIALSVWDATPKSN
                     PSAYEEAVSWTGLVNGWYNIVTFLTAFALVGLAKKLGPKKVHTFCLLLAAVGFLLFPH
                     IQNKYLLFFAITGFGIGWASMMGIPYLMVVGDIPKERYGVYMGIINMMIVIPMIIQNL
                     SFGYVLKHFLNNDPRLAITFAGALLLLSAVATLLIKSKNSIQNKN"
     gene            14337..15263
                     /locus_tag="PLQ70_02140"
     CDS             14337..15263
                     /locus_tag="PLQ70_02140"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HQE33445.1"
                     /translation="MNTIDILCVGEVLVDFIGHQTGVLINNTRDYHRYIGGSPTNVAM
                     NAARLGLNATMVASVGNDGFGDYIFERLSNVGVDTTHIKSLDDKSTSVIFVSRSDGTP
                     DFIPYREADSYLYEEQISTDTLSKTKIFHTTCFALSKNPAQELILKKAEEAFHLGCKL
                     SIDLNYAKKLWNSQAEALQVIKTYCKFNPLIKISEDDMLRLFEKELPHNEIFNFFHNE
                     GVETVCLTLGSKGVKLSQKGQEVIEMPAIKIDEVVDTTGAGDAFWSGFLFAYIKKKNV
                     TECLDVALKLAALKLQNVGRLPDNINILSNLL"
     gene            15270..17003
                     /locus_tag="PLQ70_02145"
     CDS             15270..17003
                     /locus_tag="PLQ70_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQE33446.1"
                     /translation="MLQNDKIIANGVMLNGYPDSIGEKLSDTIKMLQMPEFDGVFSLY
                     YVLPTFFNSDLDRGFSIIDYNINQDLVNTEDLKTLNKLHIKLKFDIVLNHLSVASPQF
                     KDLLKNGNQSKYKDFFINWNEFWQGNGIENEEGIIIPNQDFLNKLFMRKSGLPTLKVR
                     FPDGTEQPYWNTFYQQITYAKITTSDLETLKLTPEQHEFICKKVNDAIEKNEDFSSID
                     FENYTNLKAEIIQIVEQHRSYLGQMDVNATSPLVWEFYEETLKKLSQYGCEILRLDAF
                     AYLHKQIGESNFFNKPGTWDYLERIKQIATKNNLMLLPEIHAEYGLHLHDEVANEGYP
                     IYDFFLPGLTIHTIENKTSKALLSWAKEIISKGYKTVNMLGCHDGIPVLDLKGKEVNG
                     QYNKGLLEDNEIEGIMNTIIERGGRVKNLYDPAGNKISYYQVNATFFSALGEDEQKLL
                     LARAIQLFMPGIPQVWYLDLFAGKNNYEAADNGGSAGHKEINRTTLTMEAIEEGLKTA
                     IVKNQLEIIRLRNTSNAFLGTVTINDAFEDRIDIKWTNDTTIAHLEANLKTYNFTIHH
                     IENSITKEMNF"
     gene            complement(17000..>17709)
                     /locus_tag="PLQ70_02150"
     CDS             complement(17000..>17709)
                     /locus_tag="PLQ70_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HQE33447.1"
                     /translation="EMGFENDRRWMLIDQNNELITQREHPYLSQFYPEINEGKITISH
                     QDSTHEFFMDEHIEKPIFSKVWDDDTQVVEISKLTSQWFSDALGFSCKLVKILNNGDR
                     KHSSSRLNQTLNVSLADAFPYLLIGSKSLEFLNERLEEKVSIKRFRPNIVISSLTAHE
                     EDFFDTFQIGNVQFKNAKPCGRCVMVNNNPATAKVAKEPLKTLSTYRTSNNNVYFGTN
                     IFCLNEGIIAVGDVVNF"
BASE COUNT         6401 a         2963 c         2988 g         5357 t
ORIGIN      
        1 agtaaaatat catttccaaa aaaattgggt tggaaccagc ttgaaactgg aagacaatca
       61 agaaaaagac aaagtcaata atcaattttc ggcattaagt caaagattta cggaatatgg
      121 tttatttgct ggacgtggcg acagcaccaa agtttttgtg gaattgggtt attttaaaag
      181 agtcaacgat agtttgcaaa acggaagtat tcaacgggta aataattcgc aaactttggc
      241 tttgagatca aaattgattc aaactagtaa aagtgatttg tcgctttttg tgaattatcg
      301 tgttttaaat tttgtagatg ctacccgaaa aaacgaacct tcattgaatt ccagaatgat
      361 gtacaacgac cgttttttca atcaattagt acaatggact actgtatttg aaaccaattc
      421 aggttctatc ccacaacagg aatttactta tcttgaagtg cccgttgggc aaggtgttta
      481 tacttggaat gactacaata gcaacggaat tcaggaatta gaagagtttg aagtggctcc
      541 ttttcctgac caagccaaat atattagagt gtatttgccc aaccgaattt atattaaaac
      601 acaccaaaat aaattttcac aatccttgat tttaaacccc aatcaatggc aaaacgaagc
      661 gggtttcaag aaaatagtct cttattttta caatcaaact tcctatctcg ttgatcgaaa
      721 aattgaaaac aaaggcgaca atttcgattt aaatccgttt agcaaatcgg aggaaaatgt
      781 tttgggattg agttcaagtt tcaggaatag tttgtttttt aatcgaggaa aacaatggca
      841 ttccgtgacc tacacctatg ttcaaaacga aaataaaaat ttactttcca ttggttctca
      901 agaagccaaa aatagctcca atcaattgca atacaatcat ttatataata aaagttggtt
      961 gtttggattc tttacaaaaa ccatccaaac ctctttgaca tctgaaaatt atccagacaa
     1021 gaattatgaa ataaaaggct atcaattggc gccaaaaatc agttatttat tttccaaaag
     1081 caccagttgg gatgtatttt atgaacttca aaacaaggaa aaccaaattg gaaatttaga
     1141 aactttactc caaaatagat ttggaacctc ctttacttat gctggcaaaa agaattttac
     1201 gatgaatgga gaaatctctt tttaccaaaa taaatttgtt ggcaacgacc tttcatcagt
     1261 tggatttcaa atgcttgaag gattgcaagc aggacaaaac ctgacttggc gattgctttt
     1321 tcaaaaaaat ctaaccaatt ttctggatat aaatttcaac tatcaagggc gaaaaagcga
     1381 aactagccaa accattcata cgggaagtgt acaactgaga gcttattttt aaaatattgt
     1441 tttagaaatt taaacccgta ctattttttt ttattaactt tactcttcaa accttaaaat
     1501 caaattatta tgaaaaaatt aattttgtta tgcttccttt ttgttttctc aaacactttt
     1561 tatgctcaaa ccacaaaatc aaaaagtgaa cctacaagta aagaaaccac caaaaaaaaa
     1621 tcaagttata attacgacaa agatgttcct gtaaaatcta ccaaaaaaga ggcagattcg
     1681 aaaacaaaat caacaaaaga aaagactaaa aaagaagccg attcaaaagt caaatcaact
     1741 aaagaagcta ctaaaaaatc gggttatgat tatgacaaag atgtttctgt aaaatcaaaa
     1801 tctaataatg ccaccaaaac taacaaatcg gatgtaaaca aaattgatgc taaaataaaa
     1861 gacgaaaaat acactaaggg aaattctaac aagaaaacta cttataaaga aaccaacgaa
     1921 aaagcaccaa aaactccaga tagagtaaca ggagaataca aaggcaaaaa agtatataca
     1981 ggttctagag gagggaaata ttacatcaat aaaaacggaa ataaaaccta tattcaagac
     2041 taactcttta gaaaaatata aaactgtcca aaattatttt tttttggaca gtttttttat
     2101 tccttaactt aacggtaaat taatactttc aaaataggat ttgctgttga attcatacct
     2161 ttcgctttta atttgatgta aaaaatatga gtttcaatac tgctaacaaa accattttga
     2221 ttgccccttt aaattggggt ttgggtcacg caacacgctg cattccaatt attaaagcct
     2281 tgcaagaaaa caactttatt cccattatag cttctgatgg aattgcactt gaattattgc
     2341 aaaaagaatt tccttatgtt caaacgctta aattgccttc ttatcaaatt gaatatgcta
     2401 aaaatggcaa aaattttaaa tggaaattat tgaaaaactt tcctgaaatg attaaggcga
     2461 tttggaaaga agagaaaaaa gtcaggaaat ggattaaaaa atatgatatt gacggcataa
     2521 tttctgacaa taggctaggt gtttttagca aaaaaattcc ctctgtttat ttgacccatc
     2581 aattgaatgt aatgactgga agcaccactt ggtttaccag tatattgcac caacatatca
     2641 tcaaaaaaca cgatgaatgt tgggttcccg actttgaagg aacgtcaaat ttatcataca
     2701 aattaggtca tattgaaaat cctgatttta atttgaaata tattggagca ttgagtcgaa
     2761 tgcaaaaaaa agaaacacca aaactatatg atttgatgat tattctttct ggccctgaac
     2821 cccaacgcga attattggaa gacaaactaa aagtagaaat tctgaaatac aaaggaagtg
     2881 ttgttttcat aaaaggaatt gtcgaaaaag accaaaaaaa ggaacaaatt gaccatgtta
     2941 ctttttacaa ttttatgaat agtaggcagc tggaacaaac gtttaacgaa agtgaaattg
     3001 tgttatgtcg ctcgggttat accacgatta tggacttggt gaaattggag aaaaaagctt
     3061 attttatccc aacgccaggt cagtatgagc aagaatattt agccaaaaaa ctaaaagaag
     3121 aaggtcttgt tccttattcg aaacaagaca attttagtat agaagatcta aaggaaatag
     3181 aaaattataa aggattacca ttattggaaa tccctattaa ttggaaacat ttatttgaag
     3241 tttttgaata ataaaaactt caaataaatc tctcaattcc aattattagg ttcttgttga
     3301 ctctcgaaat cttaattcgg ttttgattac agtagtttta tacggggctt cattttctcc
     3361 tttttctaat ctttcgatca gtaattctgc tgctgcttca ccaatttctg gtccgtgttg
     3421 actaactgta gttaaacttg gagataatct tcttgaagca agaataccat cggcaaaacc
     3481 aataattgat agattttctg gtattttaaa tccttttttt agactaattc tcaaggcagc
     3541 aaccgagtca ttttcatcta aagcaaaaac gccatcaatt gatttattgt cgaataaaat
     3601 ttcggccttt tcttttaaat cctcctcaga atcggtacgg ataattaaat ctggatttac
     3661 cttaatatta ttgttttgca aagcttttaa atacccattg gctcttaatt tcccaacact
     3721 taaattatct gccgaagaaa gtaaagctat atttctacat ccaatatcta tcaaatactg
     3781 agttgcattt agagctgaat caaaatcatc aacaaccact ttatcacatt ctacttcgtc
     3841 aacaactctg tcgaacataa caattggtgt tccttcgctt attatttctt tgaagtgatt
     3901 gaattcatga agtttttgag cttcttctga tggagacaaa ataaagccat cgattgttcc
     3961 attgctcaac atatccatgg tgctaatttc tttcttcaaa gactcgttag aaatacaagt
     4021 gattacatta tatcctttct cttcggcaac tttttcgatt ccagtaaaaa ctttggcgaa
     4081 aaatgaattt aaaatattag gaatgattac gccaatggtt ttggtctttc tgttttttaa
     4141 attcagacca attacattgg gtttataatt ttttagtttt gcgtattcct taattctggt
     4201 tttggtttgt tcactaattt caggactatc atttagagct tttgaaacag tagaaacgga
     4261 aacatttagt tctttagcga tttgttttaa agtagcttta gctttcatat ataaaattag
     4321 ttttaaattg ctaaggtaat aaaaaatacg aagaatgaat tgttgcctca atgataaacc
     4381 ttaataaaat actttatttg atagaaattg ggttgtgagt aaagtcctca aagaaaatta
     4441 aaccctgttt taatttaact taccaatttc ggatagcgaa aaacaaactt ttgtattgtt
     4501 gttcaaaact aaaaagacag ccattctcat tgctgcaaca actcttttag aataaaaaaa
     4561 ggacttgctt aatggtcaaa caaatcaaac aagcttcatc aaaaaaccaa cacaaagaga
     4621 ttcaaaacaa tttgaaaagc acagtccaaa aaaacaaacc gtttacagac tgattatcag
     4681 aataaaataa aaattactga gtatttgcat ttcaaaaaaa taattgtact tttgcactcc
     4741 ctttattggg aatggaatgt ttaattaaaa taaattattg tgaacgcatt aagctacaaa
     4801 acagtatcga caagcaaagc aaccgcatct aaagaatgga tcgtggtaga cgctgacggt
     4861 cataacttag gacgtcttgc aactaaggtc gctatgatcc ttagagggaa gtacaagcca
     4921 agttataccc cacacgtgga ctgtggagat aacgtaattg ttatcaactc agaaaaaatc
     4981 aaccttacag gtaacaaact agacgaaaaa acatatgttc gtcatacagg ttatccagga
     5041 ggacaaagaa ctttaactgc taaagtattg caagcaaaaa accctgcatt attagtagaa
     5101 aaagccgtaa aaggtatgtt gcctaaaaac aaattaggag cagaactttt tagaaattta
     5161 aatgttgttg taggaccaga gcacaaacaa ggagctcaaa aacctagaac tgttaaccta
     5221 aacgatctta agtaatggga gtaattcaca aaatcggtag aagaaaaacc gctgttgcac
     5281 gtgtatatgt ttcagaagga acaggagtta tcactgtaaa caaaaaacca ttcgcaactt
     5341 atttcccaac tgcaacttta caatacaaag tgttacaacc aatgtctatg acagaaaatg
     5401 caaccaactt tgatgtaaaa gtaaacgttt acggaggagg ttcaactggt caagcagaag
     5461 ctgtaagaat ggctttagca agagtaatgt gtgaagttgg cgaaggaaac agagctatct
     5521 taaaaccaga aggtttatta acaagagacc caagaatggt tgaacgtaaa aaattcggtc
     5581 agaagaaagc tcgtaagaga ttccaattct ctaaacgtta atattgcctg tcttgtgcaa
     5641 ttcgtacaag acaaattgat aattaaatta aaaaacaatg ttgttgttgt cccgatgcgt
     5701 tgggaaatta gtttagcatc taaatgggtt gaaaagttta tagtttatgg tttatagttt
     5761 cgagttgaaa caacaaacaa taaacaacaa accaaaaaaa cacattgcta atcaacagaa
     5821 cgtaaactaa ataaaaaatg tcaaacaaag tagaagtaaa agaattacta gaagcaggtg
     5881 ttcacttcgg acacatgact agaaaatggg atccaaatat ggctccatac atttatatgg
     5941 aacgtaatgg aattcacatt atcaatctat ataaaactgc agcaaaaatt gaagaagcta
     6001 acgaagcttt gaaaaaaatt gcagcatcag gtagaaaaat attatttgtt gctaccaaaa
     6061 aacaagcaaa agacatcgtt gccgaaaaag cagcagcttg taacatgcct tacattactg
     6121 aaagatggcc tggtggaatg ttgactaact ttgtaactat cagaaaggca gtaaaaaaaa
     6181 tgtcttctat tgataaaatg aagaaagacg gaacattcaa tacactttct aaaaaagaac
     6241 gtttgcaagt agatcgtctt cgtgctaaat tagagaaaaa cttaggttca atcgcagata
     6301 tgtctagact tcctgcagca ttgttcgtag tagatatcaa agctgaacac atcgcaatta
     6361 aagaagctca aaaattaaac attccagttt ttgcaatggt tgataccaat tctgacccaa
     6421 gagaggtaga ttatgttatc cctgcaaatg acgatgcttc taaatcaatt gataaaattt
     6481 tatctattgt aactgctgcg gtagttgacg gtttatcaaa cagaggtact gaagcagata
     6541 ttcaaccaga agctgctact gaagaagtag atgctgaagt tgcaactgct gaagttgaag
     6601 ctccaacaac tgaagaataa aacaaatttt aaattccaaa ttttaaattc caaattccaa
     6661 aaaagttagt aacgtttagt tagtaatttg ttaaaattgg aatttgggat ttgaaggttg
     6721 gaatttttac ttttataaca ttaaaaacat attattatgg caacaattac tgctgcagac
     6781 gtaaataaat taagacaaac tacaggtgcc ggaatgatgg actgtaaaaa agctttagtt
     6841 gaagctgatg gagatttcga caaagccata caaaacctta gagaaaaagg acaaaaagtg
     6901 gctgctaacc gttctgaccg tgagtctagc gaaggagctg ccgtttcttt tatcaatgct
     6961 gacaaaacca aaggagctat catcactttg aactgcgaaa ctgatttcgt aggtaaaaat
     7021 gaagctttcg taactttagc taaagaatta gttgaaaaag caatcaactt ttcttctaaa
     7081 gaagaatttt tagcttcaga tttcaacgga attactgttg ctgaaaaatt aattgagcaa
     7141 actggagtta ttggcgaaaa aatcgaaatc ggtggtttcg aaattttaga aggcgctttc
     7201 gttggatctt atgttcacgt taacaaaatt gctgcattaa ctgcaatttc tgcaccaatt
     7261 gctaatggtg atgttttaac taaagacatc tctatgcaag ttgcttctat gggagctgac
     7321 accttatctt acaaagattt tgatccagct ttcgttgaat ctgaacttgc tgctcgtatt
     7381 gctatcatcg aaaaagaaaa tgaagaagct gctcgtttag gaaaaacatt gaaaaatgtt
     7441 cctaaataca tttcattctc tcaattaact cctgaagtta ttaaacaagc tgaagaagat
     7501 gctaaagcag aattaaaagc tgaaggcaaa ccagaacaaa tttgggataa aattattcca
     7561 ggaaaagtgc aacgtttcat ctctgacaac actactttag accaagagaa agccttattg
     7621 gatcaaaact tcatcaaaga tgatagcaaa aaagtgggtg attatgttaa aggattcaat
     7681 gttgaaatca caggttttaa aagagttact ttaggataat tataattcat tattcaatac
     7741 caaaaaggga gacttattag taagtctccc tttttatttt acatcctttt cctttgaaaa
     7801 tactgacatc attctaattt tattactaaa aaacaaaata caaaatttac tatatttgct
     7861 tctataaaga cttattttaa aattcacaac tacaatcaat ggctcatttc aaaaaaacac
     7921 tcctgttttt aattattttc agtttaatta gttgcggaaa aaacaaagaa agccaggaac
     7981 acgaattaga acaaaaaaaa gaacctagct acgaacaaac tatcgaaact attaatgcag
     8041 aagtccctag aaaagacacc acaaaaactt caaaagaaga cagcataata gccatcaaac
     8101 actctatttt aagagaaaaa aaatacacaa aaaagaaagc aaatactcca actgaagaaa
     8161 aaaaagtaac tgaaactaaa acagtaactg aaaacacctc agaaacaaat accactaaac
     8221 cagaaataaa aaaagtaaaa gcaccttcat ttgtttacat caaaaaaata ttaaacgaat
     8281 gtgaaacagg agtaacaatg actcaagaag atttagaagc caaatttaag attccaaaag
     8341 aggcaatgaa attagttaaa agtgtaacca aaaccgctcc agacgagctg gatattaaat
     8401 ggaaatcaac ttggctcgta gaaaaaatat ccgatgctaa attcaaagac ggaaaactta
     8461 aaatgagatt cgaagacaac aaaatgtaca cttcaggcgg tgccatctcc atcaaatatg
     8521 aaaagaaatt atactctgat ttaattttga taggacgtgc tgcatacatt cccctacagt
     8581 aaaagggttt tattggcaaa ttggaaacgg aaaataataa atatggaact tcaaagatgc
     8641 agttggtgct tatccagcga tttgtataaa aaataccacg acgaagaatg gggtgttcct
     8701 gtctatgaag accaaaaatt attcgaattc ctattgctcg aaacttttca agcgggtttg
     8761 agttggatta cgatactcaa caaaagagaa aatttccgca aggcctttga tgatttcgat
     8821 tacatcaaaa ttgccaatta ttctgaggct aaaattcaag aattgctttt ggacgctggg
     8881 attattcgaa ataaacttaa agtatattcc gctgtatcta atgcacaggc ttttataaaa
     8941 atacaagaag aatttggcag tttttccaaa tatatctggg gttttgtaga tggaaaacca
     9001 atcgacaacc atcgtaaatc actaaaagaa gtacaagctg ctactccact ttctgatacc
     9061 atcagcaaag atttaaaaaa acgtggattt aaatttgtag gctcaacagt agtttatgca
     9121 cacatgcaag ctactggaat ggtcaatgat cacgttgaag attgttggac gagaaatcaa
     9181 taggcagtgt tcagctgtaa tcaaacatat tttcaaaaaa agaaaatcat ttcttcaaag
     9241 tagattcacg cacaataaca cttgttccca actcgactat tttgggttca aaaggcttac
     9301 cattctttct acaaactatt tcttcaagca acaagccaaa agcagcaacc cccatttcat
     9361 aattgggctg atctacagta ctcaactttg gcgagattac ttgagaaaca aaccaattac
     9421 taaaaccaat tatggcaatt tggccaggca cttgtattcc gatttcattg aaatgcgcca
     9481 aaactccgat agccactaaa tctgtaatga caaaaatacc gtcaataccc aaatcatcct
     9541 caataatttg ttttgcaaat tcaacacctt gctcaaaagt aatgcttttg caggcataca
     9601 ctaatttagg atcaaaagga attccgtttt tttctaaagc ttttttataa ccaataaaac
     9661 gatcaatgga gttttgagga ttttcaagac cccgaatatg ggcgattttt ttacaacctt
     9721 tgtcaattaa atgctgaaca gcctccatag ccgccttttg gtcattaata atcactttag
     9781 aactcggaat taatttagag attttatcaa attgaacaaa aggaatttct ttccgaagga
     9841 tttccttgat gtgaaaatca ttattagatt cattagaaag tgacataata atgccatcca
     9901 ctcttttatt aatcaaaagt gccacctgct ttttttccaa ctccaaagat tcatttgatt
     9961 gcagaatgat aacgaggtat ccattttttt cggcctcggc aataattccg ttaattacat
    10021 tggagaaaaa atgatgcacc acttcaggaa ttatcaaacc aatggtttta gattctttgg
    10081 ttcgcaaatt gacagcaaaa ctattgggcg tgtagttaag ttcctcagcc aaagcataaa
    10141 cagcctttcg agttttctca cttacatccg aataattttt taatgctttg gaaaccgtag
    10201 ttatggatat tcctaaagtt tcagcaattt cttttaaggt ggtctctttc atataacaaa
    10261 tatgagaaaa atttttttat gaaatcgaaa acgttttcgg taatttcgaa aacgttttcg
    10321 attatattta tctattttaa aatggttaaa aaagtagttt tgataaaaaa ttaccaaata
    10381 aattattaac taaacaaatt taaaccatga aaaaaaacca ttcatcattt aaaaaaatcg
    10441 ggctgattat agcattattg cttttcaatt tgggtttttc acaaaatgaa actaatccag
    10501 ttgttgacac cacaaaaaca acaaaactag aagatattgt aatcactgga gttgccaacc
    10561 caaaagcaaa aatcaaatcg agtgtttcta tcactactat ggacgtaaaa caagttgaac
    10621 aatcggcacc aagatcgaca gcagaaattt ttagatccat tcctggaatt cgttctgaat
    10681 cttctggtgg agaaggaaac tcaaacattt cggttcgtgg tgtgcctatt tcttctggtg
    10741 gttctaaata tctacaaatt caagaagatg gccttcctgt acttttgttt ggagatatag
    10801 cttttgcaac agccgatatt tttactagat ttgatggaaa cacagcaaaa atcgaagcca
    10861 ttcgcggtgg atcggcctct actttatcat caaattcccc tggtggaatc attaacttta
    10921 tcagcaaaac tggaaaaact gaaggcggaa tggtaagaac cagtttcgga ttggattaca
    10981 acaattttag aaccgatgtt gactacggaa caaaaattgg tgaaggactt tattttcacg
    11041 caggtggttt ttatagagct ggcgaaggag taagaaaaac aggtgttaca tctaataatg
    11101 gtggtcaagt aaaattcaat cttaccaaag aatttgaaaa cggaaaaatt acggtttatg
    11161 ctaaattctt aaacgacaga gctgctgctt atatgccaat gccggtaaaa gttacaggaa
    11221 caaactcgaa tccaagttgg ggaagcgtta gcggttatga tgctacgact ggtactttac
    11281 aatctatcta tttgaaccat aatgttggtc ttggccctga tggtcaatta agaagagaaa
    11341 gtgttgccga tggaatgaat cctgtttcaa aatcaattgg tgtaaacgct tcatttgatt
    11401 tagaaggcgg ttggaaaata acagaaaacg gtcgttattc ttctaatagc ggtggtttta
    11461 tcgctccatt tccagcagaa gtgggttcgg cagcagcaat tgcagcttct tttggagcag
    11521 gttctaaatt gacttatgcc gattcaggaa ctccgtttaa caacccaaat ggattggttt
    11581 ctagaattca tatgttcgac actcaattaa atgatttaag caatttcatg aatgacttga
    11641 gaataaccaa aaaatttgat aaaatcggta ttactgctgg ttacttcaaa tcacttcaaa
    11701 atatctcaac ttcgtggtta tggaattctt acttacaaga agtttcagat aacaatcctc
    11761 gtttaataaa cgtaacaaat gctacgggaa cattattatc agacaacggt ttgtatgctt
    11821 atggaactcc agcttgggga aatcttgcta gaaattatga cactcaatac aatgtttctg
    11881 ctccttatgt aaacttgtct tttgatgcta ccgaaaatct ttcttttgaa ggaggtttgc
    11941 gttatgattt aggaaaagtt tcaggttcat tcgctggcgg tacatcaaga gcttatgaca
    12001 tcaacaacaa cggaacaata tcagttccag aacagaatgt ttttgctgta aacactgcta
    12061 atgaaactgc tgtgaattac gattacgatt atgtttctta tactttggga gccaactatt
    12121 tattgactgc aaaacaatct ctttttgcaa gatatagtaa aggagctgct gcaaaagccg
    12181 atcgtatttt attttcagga ttggattatt tagatggaag caaaatcaac gctttagact
    12241 ttttgacaca agccgaatta ggatacaaac ttaaattccc taaagggtat gcttatgcaa
    12301 ccgcttttca ctcaaaaacg gatgagcaag caggttatga agcctctaca aacagcttta
    12361 ttaaaaacaa ttataaatca tatggtttag aaatagaatc gtcttataat gtgatggata
    12421 acttcaattt aaaaggagct ttcacttaca ccaaagccga aattacttct ggagataacg
    12481 atggaaatga accaagaaga caaccaaaat tgatgtacaa tttgatgcca acttataact
    12541 tttcatccaa taaaaacaca gttggattga gttttattgg tcaaaccaaa gcatatgctc
    12601 aagattcaaa tcaattagtg atgaatggtt ttgtaatcgt aaacgccttt gttgaagttg
    12661 gaatcacaaa aggattatct gtaaaccttt ctggaaacaa cctatttgac accttagcaa
    12721 ttaccgaatc agaagaagga aatatcacag acaataccgt aaattatgtt agagccagac
    12781 caatgccagg aagatctctt tcaatggctt tgtcttataa gttttaaata agaaagttaa
    12841 atgttagtta gtttatagtt gccaattcct gtaccctaaa aaagtacagg aattgatttt
    12901 aaaaattaaa aaaaatagat atggcatttt ctaaacctaa actgaatttt tggcaaatat
    12961 tgagtatgaa tgttggtttt tttggcattc aatatagttt tggtttacag caaagtgctg
    13021 ttaaccctat ttatgatttt cttggagcca gtccagatca gattccattg ctaaatcttg
    13081 ctgggccagt gacaggatta ttggtacaac ctttaattgg agccttaagc gataaaactt
    13141 ggcatcctaa atggggaaga agaaaaccgt tttttttaat tggagcacta ctttgtagtg
    13201 tggcactatt attatttcct tttagcagtt cactatggat ggcggcagga ttgctttgga
    13261 ttttagatgc cggaaataat acagcaatgg aaccctaccg agcctttatt gccgataaat
    13321 tagatgaaga acaacaacct ttaggctttc aaatgcaaag tttctttacg ggtttaggac
    13381 aaacattagc caatttatcg ctgtttattt ttccgatgat aatcatagga aaaacaggtt
    13441 cgttacccac ttgggtttac gcttcctttt ttctaggagc tatttgttcc ataaccacaa
    13501 tttggtggag cattaaaacc acacccgaaa tccctccaac agaagaagag ttggcagcaa
    13561 tacgagccaa aaaaggaggc ctattcgaac cgcttatcga aataattgat gccataaaag
    13621 aaatgccaaa agtaatgtgg caattggctt tagtatatct ttttcaatgg tatgctttgt
    13681 tttgctattg gcaaaactcc tcaaaaagta ttgctttatc agtttgggat gcgactccca
    13741 aaagcaaccc ttcagcttat gaagaagcgg taagttggac tggattggtc aatggatggt
    13801 acaatattgt tacttttttg actgcatttg ctttagtagg tctcgccaaa aaattaggtc
    13861 ccaaaaaagt tcacactttt tgcttactat tagctgcagt tggattttta ttgtttccac
    13921 acattcaaaa caaatactta ctattctttg ccatcacagg atttggaatt ggatgggcaa
    13981 gtatgatggg aataccttat ttaatggtgg ttggcgacat tccaaaagaa cgatatggcg
    14041 tttatatggg tattatcaat atgatgatag tcattccaat gataattcaa aatctatctt
    14101 ttggatatgt attgaaacat ttcttaaata atgatcctcg tttggctatc acttttgctg
    14161 gagcactttt actattgagt gccgttgcaa cgcttttaat caaaagtaaa aacagcattc
    14221 aaaacaaaaa ctaaattaaa aattacgaat tacgaattaa aaaacgttgc taacaaccat
    14281 tttacaagag gaaaatacag cgttaaatta tttgtaatta cttaaaaact aaaaaaatga
    14341 atactataga catcctttgt gttggcgagg ttcttgtaga ttttattgga catcaaacag
    14401 gagttctcat caacaacacc agagattacc atagatatat tggaggctct cctactaatg
    14461 ttgctatgaa tgctgctcga ttaggtttga atgccacaat ggttgcctcg gttggaaacg
    14521 atggttttgg agactatatt tttgaaagat tatcgaatgt tggcgtagat acaactcaca
    14581 taaaatcgtt agatgacaaa tcgactagtg tcatttttgt ttctagatcc gatggaacac
    14641 ccgatttcat tccgtatcgt gaagccgatt cgtatcttta tgaagagcaa atttcaacag
    14701 ataccctatc caaaactaaa atatttcaca ccacttgttt tgctttaagc aaaaatcctg
    14761 cacaagaact cattttaaaa aaggcagaag aagcctttca tttaggttgc aaattgagca
    14821 tcgatttgaa ttatgccaaa aaactttgga acagtcaagc cgaagcttta caagtaatta
    14881 aaacctattg caagtttaat cccttaatca aaattagcga agatgatatg ctgcgtcttt
    14941 ttgaaaaaga actgccacac aatgaaattt ttaatttctt ccataatgaa ggagtagaaa
    15001 cagtttgcct gacattagga agcaaaggcg ttaaattatc tcaaaaagga caagaagtga
    15061 ttgaaatgcc agccattaaa attgatgaag tggttgatac cactggggct ggagacgctt
    15121 tttggtcggg atttttattt gcctacatca agaaaaaaaa cgttacagaa tgcttggatg
    15181 tcgctttaaa attagcggct ttaaaacttc aaaatgtggg acgattgcca gataacatca
    15241 atattttatc taatctttta taaaatacta tgctacagaa cgacaaaata atcgcaaatg
    15301 gtgttatgct taacggctat cccgatagca ttggagaaaa attaagtgac acaataaaga
    15361 tgcttcaaat gcccgaattt gatggtgtgt tttctttgta ttatgtatta cccacctttt
    15421 ttaatagcga tttagaccga ggtttttcta ttattgatta taatattaac caagatttag
    15481 ttaacactga agatttaaag actttaaaca aactccatat aaagctgaag tttgacattg
    15541 ttttaaatca tctttcggta gcatctccac aatttaaaga cttgctcaaa aacggaaatc
    15601 aatccaagta taaagacttt ttcatcaatt ggaacgaatt ctggcaagga aatggaatcg
    15661 aaaatgaaga aggcatcatc attccgaatc aagattttct gaataaatta tttatgagaa
    15721 aatcgggttt acctacatta aaagtccgtt ttcctgacgg aacagaacaa ccgtattgga
    15781 atacctttta tcaacagata acctatgcaa aaatcaccac ttctgattta gaaactttaa
    15841 aattaacacc cgaacaacat gaatttattt gcaaaaaagt aaatgatgcc atagaaaaaa
    15901 atgaagattt tagcagtatt gattttgaaa actacactaa tttaaaagct gaaataatac
    15961 aaattgtaga acaacatcgt tcgtatctcg ggcaaatgga tgtgaacgcc acatcgccat
    16021 tggtttggga attttatgaa gaaaccctaa aaaagttaag tcaatatggt tgcgaaattc
    16081 ttcgtttaga tgcttttgct tatttgcaca aacaaattgg cgaatccaat tttttcaaca
    16141 aacccggtac ttgggactat ttagaacgca ttaaacaaat agctaccaaa aataatttaa
    16201 tgcttttgcc tgaaattcac gctgaatacg gattgcatct gcacgacgaa gtggccaatg
    16261 aaggctatcc catttatgat tttttcttac ccggattgac aatacacacc attgaaaaca
    16321 aaactagtaa agcattactt tcttgggcaa aagaaattat ttccaaagga tataaaacag
    16381 taaatatgct ggggtgccac gatggaattc ctgttttgga tttaaaagga aaagaagtta
    16441 atggtcaata caataaaggt ttattagaag ataatgaaat cgaaggtatt atgaatacca
    16501 tcatagaacg tggtggtcgt gtgaaaaatc tctatgatcc tgcagggaac aaaatttcat
    16561 attatcaagt aaacgctact ttttttagtg cattgggaga ggacgaacaa aagttgctat
    16621 tagccagagc catacaatta tttatgcctg gaattccaca agtttggtat ttggatcttt
    16681 ttgctggaaa aaataattat gaagctgccg ataacggcgg tagtgcaggt cataaagaaa
    16741 taaaccgcac cacattgact atggaagcca ttgaagaagg gctaaaaaca gcaattgtaa
    16801 aaaatcagct tgaaatcatt agactcagaa atacttcgaa tgcttttttg ggaacagtca
    16861 ccataaacga tgcttttgaa gacagaattg acattaaatg gacaaacgac acaaccatag
    16921 cccatcttga agcaaaccta aaaacctata atttcacgat tcatcatatt gaaaatagca
    16981 tcacaaaaga aatgaatttt tagaaattca caacatctcc aacagcgata atcccttcat
    17041 tcaaacagaa tatattggtt ccaaaataaa cattattatt cgaggttcga taggtgctta
    17101 aggtttttaa cggttcttta gcaacttttg ccgttgcagg attattgttg accatcacgc
    17161 atctgccgca aggtttggcg tttttaaatt gtacatttcc aatttgaaag gtatcaaaaa
    17221 aatcttcttc gtgagcggtt aaactactaa taacaatatt gggtctgaat cgtttaatgc
    17281 tcactttttc ttccaatctt tcgtttaaaa attccaaact tttagagcca attagcaaat
    17341 atggaaaagc gtcggcaagg ctcacattca aggtttgatt caatcgggaa ctgctgtgtt
    17401 ttcgatcacc attatttaaa attttaacca atttacaact aaaacccaaa gcatcgctaa
    17461 accattggga agtcaattta cttatttcaa caacttgagt atcatcgtcc caaactttcg
    17521 aaaaaatagg tttttctata tgttcatcca taaaaaattc gtgggttgaa tcctgatgcg
    17581 aaatggttat tttaccttcg ttaatctcgg gataaaactg acttaaataa gggtgttcgc
    17641 gctgagtaat caattcatta ttctgatcaa tcaacatcca tctgcggtca ttttcgaaac
    17701 ccatttctt
//
LOCUS       DAOSNN010000021        17214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3338_length_17214_cov_5.511452, whole genome shotgun sequence.
ACCESSION   DAOSNN010000021 DAOSNN010000000
VERSION     DAOSNN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 17214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17214
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3338_length_17214_cov_5.511452"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PLQ70_02155"
     CDS             complement(<1..1319)
                     /locus_tag="PLQ70_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQE33448.1"
                     /translation="MTPLKRFYNLLELDKKDVLQIFFYAIFAGLISLSLPLGIQAIIN
                     FIQSGRVSASWIVLIFLVVIGVALAGILSLMQLRITENLQQKIFVRSSFEFASRLPKI
                     KFEELYSHYAPELANRFFDTLTIQKGTSKLLIDFSAALLQIAFGIILLSLYHPFFIVF
                     GILLFFLLYFIFKFSYKSGLETSLKESKFKYKVATWLQEVARNNFSFRNELNYNYALQ
                     KNDELANDYLNYREKHFNVIKRQFIQLILFKVIITASLLSIGGYLVLSQEMNIGQFVA
                     AEIIILLVINSVEKIIIGLETFYDVLTAVEKIGQVTDLDLEEETEESYDNCYTNISLE
                     TENLTFKFPDTKQNTLKSIDLKMNQGERIFVDGINGSGKTTLIRILSGLLQPSSGSFY
                     INDDTFKKINLKQYRAQIGSIIYGETLFEGSILDNITFKDLSITQED"
     gene            complement(1340..2014)
                     /locus_tag="PLQ70_02160"
     CDS             complement(1340..2014)
                     /locus_tag="PLQ70_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQE33449.1"
                     /translation="MQLILSNIKIQVNKNLYVKDPETSDLGKKIIKNSILLIDEVGLE
                     HFTFKKLGEKIGSNESSIYRYFDNKHKLVMYLSAWYWSWIEYRLVFTTANISNTIEKL
                     EKAITIVTEKIKDDSSTEHIDEAVLNKIIISEFNKAIYTKEVDEDNKEGCFLAYKNVI
                     NRIVAIVEEVNPDYPFPKSLVSTIVEGSLHQHFLNDHLKTITNCNESISVTAFYLDLM
                     NRALKK"
     gene            complement(2139..3656)
                     /gene="gpmI"
                     /locus_tag="PLQ70_02165"
     CDS             complement(2139..3656)
                     /gene="gpmI"
                     /locus_tag="PLQ70_02165"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQE33450.1"
                     /translation="MNKKVILMILDGWGNSPDPKVSAIDNANIPFIKSLYTKYPVGQL
                     RTDGLNVGLPEGQMGNSEVGHMNLGAGRIVYQDLAKINLAVENNSMAQEQVLVDAFQY
                     AKDKNVNLHLLGLVSDGGVHSHTSHLRGLIDATQAFGLEKVFVHAFTDGRDVDPKSAK
                     TDIQNLENHIANTSTKIASVVGRYYAMDRDKRWERVKIAYDLVVNGTGTHSKNAVKSI
                     EESYENNVTDEFINPIIMVDENDKPLATIKEGDVVVFFNFRTDRGRELTETLSQRDFH
                     EQNMHKLDLYYVTLTNYDETYENVKVVYNKDNVTETLGEVLEKANKKQIRIAETEKYP
                     HVTFFFSGGRELPFEGESRILKNSPKVATYDLQPEMSAYELADALVPELNKGEVDFVC
                     LNFANGDMVGHTGVMSAAIQACEAVDKCVEKVITAALANDYTTLIIADHGNCETMINP
                     DGSPNTAHTTNPVPIILVDKELKSIQDGVLGDIAPTILDLMKIEQPAVMTRHSLL"
     gene            complement(3932..6358)
                     /locus_tag="PLQ70_02170"
     CDS             complement(3932..6358)
                     /locus_tag="PLQ70_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HQE33451.1"
                     /translation="MPKYCSIFILFLIFISFCTYGQKKTLRAKNITESITIDGKINEE
                     AWKTAPIATDFVMYEPDNGKPIGEDKKTDVKVLYDNNAVYISAVLYDNQPNKISRELT
                     NRDEYGVSDVFSVSINGNNDGQQDFRFFVTAAGVQLDCLATEDNEDFNWDAIWDSEVT
                     MTNFGWVVEMKIPYAALRFSKATKQTWGLNFRRDLKRDDKDQSYTWNFVDTKIGAVIT
                     QEGILEGIENIKTPTRLFLIPYTSAYYQQDDFASDKTLKAGMDIKYGINDAFTLDAIL
                     VPDFGQTKFDNAILNLEPFEQQFDENRSFFTEAADLFSKGNLFYSRRIGGAPTIEPTT
                     GDNEVVTEYPSSTDLLNAVKVSGRTEKGLGIGFLNAITEKTYAKIQDTISGATRKEIV
                     EPLVNYNVLVLDQRFNQNSSVTFVNTNTLRNGNFRDANVSALLFDLNTKVNTYKLSGD
                     FKYSAVKDSDINYGSIYGLENYDGFKTQLSFAKTSGKYRYMFSGKYLSEMYDVNDLGI
                     IFVNNYHSGYGEMSYRIINPTKTFNSLKFSQNINVEIQNKTGKAQEAWYQAKLAAITL
                     KNNYLEGKFQINPIDRYNFYEPRVYGRYVYVPKSVTGYFRYSTNQNNKFSLDIDGSIE
                     KFDEDGRVVYAISPGLKHRFNDKFSLSYNLIYTRKVNDRGWVDFDADDIIFAERNREI
                     VDHYFTGKYSISNKMSLNLIARYYWSYAENHEFFSLQDDGKLIANSTYSENRNRNFNS
                     WNFDFSYSWWFAPGSEISVLYRNYALESTNLVQKSISTNLNNVFDTNLTTIFSVSIRY
                     YIDYNSLKRK"
     gene            6549..7262
                     /locus_tag="PLQ70_02175"
     CDS             6549..7262
                     /locus_tag="PLQ70_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HQE33452.1"
                     /translation="MIYKIFPVSLFLLISIFLNNTNPVKTQKPVIASVLTPNADLDIE
                     TKYSALNANQFSLPNIESFKEALKGFYELKERGLVQKDILTLVDFSLSSNVKRLWVID
                     LDTNTILYHSLVAHGRNTGEEYANSFSNSNSSYKSSLGFYLTGEVYNGKHGMSLKLDG
                     LEKGINDNARERGVVMHSANYVSNSFIKSNKRLGRSQGCPAIPEELLKDIVNTIKNKS
                     CLFIYHPSRSLEYMKPLFS"
     gene            complement(7251..8834)
                     /locus_tag="PLQ70_02180"
     CDS             complement(7251..8834)
                     /locus_tag="PLQ70_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQE33453.1"
                     /translation="MNKIFILLLVFVAFGCKKELKIKEVKPFPAAISIIQVEGKSVFI
                     DSILLEPFRSKVLKEFYRATSYKSVWQSKENRKFILKSFLNSDGEGLNPERYNVNKLQ
                     NFEEKYAVLKDKERVSYDILLTHSFQKYISHLSNGQIHHKYLYKNWDLKENNVDVNET
                     IASMLKSDSLPFKIEQLKPNHIVYKSLKKALQIINSFPNDDFTTIEIANKIVLNDTNP
                     SLIAIKKRLIYWKDMKPKDSLSAIYDSETNEAMKKFQIRHGLSADGVIGKGTVDALNF
                     SKRKRKEQILTNLERWKWFPREMGEKYVIVNIPDYKLSLVKENDTLRTHKVVVGRDKR
                     PTPVLSSKINQIILNPTWTVPPTILKEDMIPAISRNRNYLAQTNIKIYDSSGNLVSAN
                     DWKPSKAKKYRYVQSPGKSNSLGMVKINFPNRFSVYLHDTNHRDFFVKQKRSLSSGCV
                     RVENPLKLAETLLNDTVNWNAEKITKILQSEKTKYIKINDDVNVHILYWTAWSEGNTL
                     IFRDDIYNLDADLYQKLGK"
     gene            complement(8837..9277)
                     /locus_tag="PLQ70_02185"
     CDS             complement(8837..9277)
                     /locus_tag="PLQ70_02185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE33454.1"
                     /translation="MTTIIKEIPYLETFSVRHPVLRAGKPIESCRFDGDDLIATHHFG
                     LYLNEELVGIISLFLTKNLVFKEQKQYQIRGMAVLEQYQKSGFGKMLILYSEAFLNTQ
                     KVDLIWFNARVEAVGFYEKMGYQKTGKIFEIPDVGEHIVMFKKN"
     gene            9399..9471
                     /locus_tag="PLQ70_02190"
     tRNA            9399..9471
                     /locus_tag="PLQ70_02190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9432..9434,aa:Gly,seq:tcc)
     gene            9582..9754
                     /locus_tag="PLQ70_02195"
     tRNA            9582..9754
                     /locus_tag="PLQ70_02195"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9615..9617,aa:Gly,seq:tcc)
     assembly_gap    9633..9732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9881..10588)
                     /gene="pepE"
                     /locus_tag="PLQ70_02200"
     CDS             complement(9881..10588)
                     /gene="pepE"
                     /locus_tag="PLQ70_02200"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HQE33455.1"
                     /translation="MKNLIIASTSTLHGGEYLEYLLPELEKHFQYCKTILFIPFARPS
                     GISHEEYAFKVASAFAKIGKEIKGIHEVDDYSDAIQKAEGIFTGGGNTFLLVTQLYKK
                     DCMIALAEAVKNGTPYLGTSAGSNICGLTMQTTNDMPIVYPPSFKTLGLIPFNLNPHY
                     LDADMQSKHMGETRETRIAEFHSFNTIPVLGLREGSWLEVKGEKVVLKGGLKARLFRQ
                     NQIPVEMDSESDLSFLK"
     gene            10734..11510
                     /locus_tag="PLQ70_02205"
     CDS             10734..11510
                     /locus_tag="PLQ70_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQE33456.1"
                     /translation="MIHPLMKLGICMFFVLIANTVLAQESPRKILNGKINANTMDLEG
                     IYVINLKTEKSTITEKEGYFSISTMPGDTLLFSAIQLKEKRIALESKDFKEELLQVKM
                     ETNITTLKEVVVKRYDNINAVSLGISPSGIKHLTQAERHLKTASDLDPTANAGSMTGG
                     SIGLDPLLNLISGRTAMLKKELVVEGKLSFINQMENMFNEDYFKKTLKIPAEYIKGFL
                     YYIVDNDRFTNILKSKNKTQIEFAMVSLAEKYNQILLNEK"
     gene            11500..12261
                     /locus_tag="PLQ70_02210"
     CDS             11500..12261
                     /locus_tag="PLQ70_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQE33457.1"
                     /translation="MKSKIPLLVMLLFSPIILGQTAAEKIIHGKIIIESHPISGVTII
                     NLVNEKSTVSDSNGEFYILAKADDLLVFTSVNLEYHRKQIEEEDLKQDIITIKMTAKI
                     TELDEVIINKHPEINAVALGISPAGIKKYTPAERRLKTAGEFKPIQLLGLLGGSMPLD
                     PLINSISGRTAMLKKELEVEKKELLLVQIDALYDNEYFVQKLKIPEDYINGFKHYIIE
                     NEKFTAVLKSKNKKMGGFLMIDLAREYNQIILNEK"
     gene            12333..12506
                     /gene="tatA"
                     /locus_tag="PLQ70_02215"
                     /pseudo
     CDS             12333..12506
                     /gene="tatA"
                     /locus_tag="PLQ70_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259714.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            12779..13354
                     /locus_tag="PLQ70_02220"
     CDS             12779..13354
                     /locus_tag="PLQ70_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQE33458.1"
                     /translation="MLDKIQTLDTKLFIYLNGLGSENFDGLWLIITKQIYWTPLFLLL
                     FYIIFKELGTKQSLYLLLFVAVLLVFTDQITNLFKNGFQRLRPCNNPEINTIIRIVQS
                     RKSFSFFSGHAANTMAVATFLYLIFKKDFKYFGLLFLWPLIFAYSRIYLGLHYPIDII
                     SGYFCGAILGYLMFKLYRIAQKRNFSVKLKK"
     gene            complement(13370..14011)
                     /locus_tag="PLQ70_02225"
     CDS             complement(13370..14011)
                     /locus_tag="PLQ70_02225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HQE33459.1"
                     /translation="MHFISQDLENYIEQHSEKEPELLAALNKETYQKVLLPRMLSGHF
                     QGRVLSMLSKLIRPVNILEIGTYTGYSALCLCEGMQENGTLHTIDIKEELVDFQRKHF
                     DKSPWGNQIVQHLGEALDIIPTLDMKFDLVFIDADKENYINYFELIVPKMNQGGIILS
                     DNVLWSGKVLEPLSPKDISTKILLEYNLLLKNDSRVETVLLPIRDGLTVSRVL"
     gene            14112..15155
                     /gene="rlmN"
                     /locus_tag="PLQ70_02230"
     CDS             14112..15155
                     /gene="rlmN"
                     /locus_tag="PLQ70_02230"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQE33460.1"
                     /translation="MQIEKKDIRALSKEQLREFFVANNDKAFRGNQVYEWLWSKGAHS
                     FEDMTNVSKTTREMLENHFVINHIKVDKMQRSEDGTVKNAVRLHDGLVVESVLIPTNT
                     RTTACVSSQVGCSLDCNFCATARLKRMRNLEPAEIYDQVIAIDKESRLYYNHPLSNIV
                     FMGMGEPLMNYNNVMKAIEMITSNEGLGMSPKRITVSTSGVPKMIKKMADDEVKFNLA
                     VSLHSAIDEIRSRIMPFSENFPLVDLREALEYWYRKTKSKVSYEYVIWKGINDNKASV
                     DALVKFCKYVPCKVNLIEYNPIDDGEFQQASEESINAYIKALEATGIVVKVRRSRGKD
                     IDAACGQLANKEA"
     gene            complement(15243..15461)
                     /locus_tag="PLQ70_02235"
     CDS             complement(15243..15461)
                     /locus_tag="PLQ70_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33461.1"
                     /translation="MKNNLLKYYITAFYLCSTFVMFAQPTADDTTGTLESGDTPAPID
                     DYVWVLALIGLFFIFMKFKAMLNNKIQE"
     gene            complement(15514..>17214)
                     /locus_tag="PLQ70_02240"
     CDS             complement(15514..>17214)
                     /locus_tag="PLQ70_02240"
                     /inference="COORDINATES: protein motif:HMM:NF033708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS sorting signal type C domain-containing
                     protein"
                     /protein_id="HQE33462.1"
                     /translation="GKIISNTFDAATNKTTVIAISPPLILITTWNGTSWSDGVPTAST
                     EAVIEGNYNTTTNGSFNAKKLTVNSGSLTINSGTIITVQNEVVNNAGTNGFVIQNNGN
                     LIQANNTTNTGNVVVNRNSNALYRLDYTIWSSPVAGQNLAAFSPLTSQTPSRFYNFNT
                     TYNTAGVYGAFSVIANPAVTNFAAGSGYLIRMPNTDLTSGYDAGTATLAFPGQFIGVP
                     NNGDITVPLVDGGAVGLRFNLVGNPYPSPINLYTFVTENTAKIQTTLYFWRKTNGVGT
                     AYCTYIPTSPTAGTYVTNGNSLSVNPASILQIGQGFFVEAKTGQTSILFKNSQRWGNV
                     AGQFFKTKQIAAADKVWLNATNAAGDFSQMAVTYFDGATPGVDAFDGKYINDSPLALT
                     SNINNEEYTIQGRPAFDASDVVSLNFKTAVAGDYTIAIDHSEGVFAAGQSVILTDAIT
                     GTETDLTTSSYTFTAATGSASARFSLKYQKTLKLDASVTNDNSVIVYKNNGVIYVNSA
                     AKAINNIKVYDIQGRLIAEQNNVKANAAVINNLKAIHQVLIVKVTSEDNKVVSKKVEN
                     "
BASE COUNT         5691 a         3013 c         2720 g         5690 t
ORIGIN      
        1 aaatcttctt gtgtaataga caaatctttg aaagtgatat tgtctaaaat actgccttca
       61 aaaagggttt cgccataaat gatgctacca atttgggcgc gatattgttt gagattaatt
      121 ttcttgaaag tatcatcatt aatataaaac gaaccagaac taggctgtaa aagtcccgaa
      181 agaattcgga ttaaggttgt ttttcccgag ccgttgattc catcaacaaa aatgcgttct
      241 ccttggttca tttttaaatc gatgctcttc aaggtgtttt gtttggtatc tggaaattta
      301 aaagttaaat tctcagtttc caaactgata ttagtatagc aattgtcata agactcttct
      361 gtttcttctt ctaaatccaa gtcggttact tgtccaattt tttcgacagc ggttagtacg
      421 tcgtagaaag tttctaaacc aatgatgatt ttttcgacag aatttataac gagcaaaata
      481 atgatttctg ctgccacaaa ttgtccaatg ttcatttctt gcgacaatac caaatagcct
      541 ccaatagaca acaaactggc agttataatg actttaaaaa gtattaattg aatgaattgt
      601 cttttgataa cattgaaatg tttttctctg taatttaaat agtcattggc caattcgtcg
      661 tttttttgca aggcataatt gtaattcaat tcgtttcgaa aactgaaatt atttctcgca
      721 acttcctgca accaagtcgc tactttgtat ttgaatttgg attcttttaa actggtttct
      781 aaacctgatt tgtatgagaa tttaaaaata aagtacaata gaaagaacaa caaaattcca
      841 aaaacgatga aaaaaggatg gtagagcgaa aggagtataa ttccgaaagc aatttgaagc
      901 aaggctgccg aaaaatcaat cagtaatttc gaagttcctt tttgaatggt taaagtgtcg
      961 aaaaaacggt tggccaattc gggcgcatag tgactataca attcctcgaa ttttatttta
     1021 ggcaatcggc ttgcaaattc aaaagacgaa cgaacaaata ttttttgttg taaattctct
     1081 gtgattcgaa gctgcattaa cgataagatt cccgcaagtg caacgcctat aacaactaga
     1141 aaaattaaaa caatccaaga agcactaact cgtcctgact gaataaaatt gataatcgct
     1201 tgaatgccta aaggcaaaga caagctgatg agtcctgcaa aaatagcata gaaaaaaatc
     1261 tgtagcacat cttttttatc taattcgagt aagttgtaaa aacgctttaa tggtgtcata
     1321 ataaatacga agtttgtttt tattttttta gtgctctatt cattagatct aagtaaaaag
     1381 cagttacgct aatgctttcg ttgcaattgg taatggtttt taaatgatca ttaagaaagt
     1441 gctggtgcaa actcccttca acaatggtcg aaactaagct tttgggaaaa ggatagtctg
     1501 gatttacttc ttctacaata gccacaatgc gattgatgac atttttgtag gccaaaaaac
     1561 agccttcttt attgtcttca tcaacctctt tggtataaat tgctttgttg aattccgaga
     1621 tgataatttt attcaaaaca gcttcgtcaa tatgttctgt gctgctatcg tcttttatct
     1681 tttcggtcac aatagttatg gctttttcta gcttttcaat agtgttagaa atgtttgctg
     1741 ttgtaaaaac caatcgatat tcaatccaac tccaatacca agccgacaaa tacattacca
     1801 gtttgtgctt gttgtcgaaa taacgataaa tcgaactttc gttggagcca attttttcgc
     1861 ctaatttttt aaaagtaaaa tgttctaatc caacttcgtc aattagaaga atactgtttt
     1921 taataatttt cttgcccaaa tcagaagtct cagggtcttt tacatacaag tttttgttga
     1981 cctgtatttt tatgtttgat aatattaatt gcataaataa tttatttaag cgcaaatata
     2041 atagtaaaac tattgatttt tgatttttta actttcattt aataaaaaaa actgcccgaa
     2101 tcggacagct ttcttaaaaa attttttttt gtaacttgtt acaaaagaga atgtcttgtc
     2161 ataactgctg gttgttctat tttcattaaa tcaagaatcg ttggagcaat atcgcctaat
     2221 actccatcct gaatagattt caattctttg tcaactaaaa taataggcac tggatttgtt
     2281 gtgtgtgctg tatttggaga cccatcagga ttaatcatcg tttcgcagtt tccgtgatcg
     2341 gcgatgatta atgttgtgta atcattggcc aaagctgcag taattacttt ttctacacat
     2401 ttatctacag cttcacaggc ttgaattgca gcactcatta ctcctgtatg tcctaccata
     2461 tcaccgttgg caaaattcaa acaaacaaaa tcaacctcgc ctttattcaa ttctggaacc
     2521 aatgcatcgg ctagttcata agcactcatt tcgggttgca aatcataagt agctactttt
     2581 ggagagtttt tcaagattcg tgattcgcct tcaaaaggca attctctacc accagaaaag
     2641 aagaaagtca cgtgaggata tttctcagtt tcggcaattc tgatttgttt tttgtttgct
     2701 ttttctaaaa cttcaccaag agtttctgta acattatctt tattgtaaac cacttttaca
     2761 ttctcataag tttcgtcata attggtaagc gtaacgtagt acaaatcaag tttgtgcatg
     2821 ttttgctcat ggaaatccct ttgcgaaagt gtttcggtca attcacgacc tctatcggtt
     2881 ctgaaattaa agaaaacaac aacatcacct tctttaattg tagccaatgg tttatcgttt
     2941 tcatccacca tgataatcgg gttgataaat tcgtcggtca cattattttc ataactttcc
     3001 tcgatgcttt tcacggcatt tttagaatgt gttcctgttc cgtttaccac taaatcgtag
     3061 gcaattttaa ctctctccca acgtttatca cgatccattg cataatatct tcccactacc
     3121 gaagcaattt ttgtagaagt attggcaata tgattttcta aattttgaat atctgttttt
     3181 gctgattttg gatctacatc tcgtccatca gtaaaagcgt gaacgaaaac tttttctaat
     3241 ccgaaagctt gagttgcatc aattaatcct ctcaaatgag aagtgtgtga atgcacacca
     3301 ccatcagaaa ctaggcccaa aaggtgtaaa tttacatttt tgtcttttgc atattgaaag
     3361 gcgtcaacaa gcacttgttc ttgtgccatt gaattatttt cgacagccaa attaatttta
     3421 gccaaatctt gatatacaat tctacccgca ccaagattca tgtgtcccac ttcggaattt
     3481 cccatttgac cttctggtaa acctacgttc aaaccatctg ttcgaagttg tccaacagga
     3541 tattttgtat ataaactttt gatgaaagga atgtttgcat tgtctattgc agacactttt
     3601 gggtcaggag aatttcccca accgtcaaga atcataagga ttactttttt gttcattttg
     3661 ataaattttt agacaaagat aaagctttcc aaaaaaatat aagtacgtca aaactactat
     3721 tatagataaa aaagagaact gcaacaaata cttaaaaatc tgttgtaaaa tatggttttt
     3781 tatacaatat atgcaacaaa aaatcttcga aaaccatgta gaaaaaagat ggtttttgag
     3841 gactattttg aaaaatttaa gggaattaaa tctctaacag cttgttttgg atattctact
     3901 atttgagaga ctatttttca agtaaaattt attattttct tttcaaggaa ttgtaatcaa
     3961 tataataacg gatactaaca gaaaaaatag tagtcaagtt ggtatcaaaa acattattaa
     4021 gattggttga tatacttttt tgcacaaggt ttgtactctc taaagcataa tttctatata
     4081 aaactgagat ttcgcttcca ggagcaaacc accaagaata ggaaaagtcg aaattccaag
     4141 agttaaaatt cctatttcta ttttctgaat aggtagaatt agcaattaat tttccatcgt
     4201 cttgaagact gaagaactcg tgattttcgg cataagacca ataatatctt gctattagat
     4261 ttaatgacat cttattagat atggaatatt ttcctgtaaa ataatgatct actatttcgc
     4321 gattgcgttc cgcaaaaatg atatcgtctg catcaaaatc tacccaacct ctatcattca
     4381 cttttcgggt atatataaga ttgtaagaca acgaaaattt atcgttgaat ctgtgtttta
     4441 aacctggcga aatggcatat acaactctac catcttcatc aaatttttcg attgaaccat
     4501 caatatccaa actaaattta ttgttttgat tcgttgaata gcgaaaataa cctgttacac
     4561 ttttaggaac ataaacgtat ctaccataaa cacgaggctc ataaaaattg tacctatcta
     4621 tgggattaat ttgaaatttt ccttccagat aattattttt taaggttata gctgccaatt
     4681 ttgcctgata ccaagcctct tgtgcttttc ctgttttgtt ttgaatttct acatttatat
     4741 tttgagaaaa tttcaaagag ttaaaagtct ttgtgggatt gatgattctg taactcattt
     4801 ctccataacc cgaatgataa ttattcacaa aaataatccc taaatcattg acatcataca
     4861 tctctgataa atatttccca gaaaacatat atctatattt tccacttgtt ttggcaaaac
     4921 ttaattgtgt tttgaaacca tcatagtttt ctaaaccgta aatagaaccg taattgatgt
     4981 cgctatcttt tactgcactg tatttaaaat ctcctgataa tttgtaagta tttacttttg
     5041 tattcaaatc gaataataaa gccgaaacat tggcatcccg aaaatttcca tttctaaggg
     5101 tattggtatt cacaaaagtc accgatgagt tttgattgaa tcgttggtct aaaaccaaaa
     5161 cattgtaatt aaccaatggt tcgacaattt cttttcgggt tgctcctgaa atagtatctt
     5221 gaattttagc gtatgttttt tcggtaatgg cattcaaaaa tcctattcca agtccttttt
     5281 ctgttctacc cgaaactttt actgcattca acaaatcggt cgaacttgga tattcagtaa
     5341 ctacttcatt gtctcctgtt gtaggttcta ttgttggtgc accaccaatt cttcgggaat
     5401 aaaaaaggtt cccctttgaa aacaaatcag cagcttcggt aaagaaagat ctattttcgt
     5461 caaattgttg ttcgaaaggt tcaagattca aaatggcatt atcaaatttg gtttgaccaa
     5521 aatcaggaac cagaatagca tcgagtgtaa aggcatcatt tattccatat ttaatatcca
     5581 ttcctgcttt taaagtttta tcagaagcaa aatcatcttg ctgataatac gctgaagtat
     5641 aagggattaa aaatagacgt gttggggttt ttatgttttc gattccttct aaaattcctt
     5701 cttgagtaat tacagcacca atttttgtat cgacaaaatt ccaagtataa ctttggtctt
     5761 tatcatctcg tttcaaatca cgtctaaaat ttaaacccca agtttgtttg gttgctttcg
     5821 aaaatcgcaa tgcggcataa ggtattttca tttctacaac ccaaccaaaa ttggtcattg
     5881 ttacttcgct atcccaaata gcatcccaat taaaatcttc attatcctca gttgccaaac
     5941 aatctaattg aactccagct gctgtaacaa aaaatcgaaa atcttgttgc ccatcattat
     6001 ttccattgat agaaaccgaa aaaacatcag aaactccata ttcgtctcta ttggttaatt
     6061 ctctagaaat tttattaggc tgattgtcat ataaaactgc cgaaatataa acagcattat
     6121 tatcatacaa aacttttaca tctgtctttt tgtcttcgcc aattggcttt ccgttatcag
     6181 gctcatacat aacaaaatca gtcgcaattg gagcagtttt ccatgcttct tcatttattt
     6241 ttccgtcaat agtaatgctt tccgtaatgt ttttagcccg aagtgtcttt ttttgtccat
     6301 aagtgcaaaa acttataaaa attaaaaaaa gaataaagat tgaacaatat ttaggcataa
     6361 tttgtgagaa tggttaggca aaaatagtaa aattaacaca aaaaacaagt tgtgcatagt
     6421 atttttcaac atcattttag aactaaaaag tagccaaatc atattttttt cataaaattc
     6481 taaaaaaaaa gctcaaacct gttgtatatt tgcaacccca agccttttgt ctaacctaaa
     6541 cgttatcaat gatttataag atatttcccg ttagtttatt tttgctgatt tctatatttc
     6601 taaataatac taatcccgta aaaactcaaa aaccagtaat tgcgagtgtt ctaactccaa
     6661 atgccgattt agacatcgaa acaaaatata gtgccctaaa tgctaaccaa ttctcattac
     6721 ccaatattga aagttttaaa gaagccctaa aaggttttta tgaacttaaa gagagaggat
     6781 tagtccaaaa agatattttg actttggtag atttcagttt atcttcaaac gtaaaaaggc
     6841 tttgggtaat cgatttggac accaacacta ttttatacca ttctttagtt gcacacggta
     6901 gaaatactgg ggaagaatat gccaacagtt tttcgaattc caatagctct tacaaaagta
     6961 gtttgggatt ttatttaact ggcgaggttt ataacggaaa acacggaatg tctcttaaac
     7021 ttgacggatt agaaaaagga attaatgaca atgcaagaga aagaggtgtg gtaatgcatt
     7081 cggccaatta tgtttcaaat tcttttataa aatccaacaa aagattaggc agaagtcaag
     7141 gttgccctgc tattccagaa gaattactta aagacattgt gaacacaatc aagaataaat
     7201 cttgcctttt tatttaccac ccatccagaa gtttagaata catgaaacca ttattttcct
     7261 agtttttgat ataaatcagc atctagatta taaatatcat ctctaaaaat aagtgtgttt
     7321 ccttcgctcc aagcagtcca atataaaata tgaacattga catcgtcatt tattttaatg
     7381 tacttagtct tttcgctttg aagaatttta gtaatttttt ctgcattcca atttaccgta
     7441 tcattcaata aggtttcggc caattttaac ggattttcaa ctctcacaca acccgaactc
     7501 aaagaacgtt tttgttttac aaaaaaatct ctatgattgg tatcgtgcaa ataaaccgaa
     7561 aaccgatttg gaaaattaat tttaaccatc cctaacgagt ttgatttccc tggactctga
     7621 acatagcgat actttttggc tttggatggt ttccaatcat tagcagaaac taaatttccg
     7681 ctactgtcat atattttgat attggtttgt gccaaatagt ttcgatttct tgaaatggca
     7741 ggaatcatat cttctttcaa aatagttggt ggtactgtcc aagtaggatt caaaataatc
     7801 tgatttattt ttgaagaaag aaccggagtg ggtcttttat cacgacctac aacaacttta
     7861 tgcgttcgta aagtatcgtt ttcttttact aaactcaact tataatctgg aatattgact
     7921 atcacatatt tctctcccat ctctcttgga aaccatttcc aacgttcgag attggttaga
     7981 atttgctctt ttcgttttcg ttttgaaaaa ttgagtgcat caactgttcc ttttccaata
     8041 acaccatcag ctgataatcc atgtcggatt tggaattttt tcatcgcttc attcgtttcg
     8101 ctgtcgtaaa tagcagacaa actatctttt ggcttcatat ctttccaata aattaatcgt
     8161 tttttaatgg ctattaaaga gggatttgta tcatttaaaa caattttatt ggctatttca
     8221 atggtagtaa aatcatcatt tggaaaagaa ttgatgattt ggagtgcttt tttcaaactt
     8281 ttatacacaa tgtgattggg cttaagttgt tcaattttaa atggcaaaga atcacttttt
     8341 aacattgaag caatagtttc gtttacatca acattattct ctttcaaatc ccaattttta
     8401 tacaaatact tatgatgaat ttgcccatta ctcaaatgcg aaatgtattt ttgaaagctg
     8461 tgcgttaata aaatatcata acttaccctt tctttatctt ttagaacggc atatttttct
     8521 tcaaaattct gcaatttatt tacattatac cgctctggat ttaagccctc tccatcagaa
     8581 ttcaagaaac tcttcaatat aaatttccta ttttctttgg attgccaaac cgatttatag
     8641 ctagtagccc gataaaactc ttttaatact ttactgcgaa aaggctctaa caaaatacta
     8701 tctataaaca ccgattttcc ttctacttga ataatagaaa ttgcagcggg aaatggcttt
     8761 acttccttta ttttcaattc ttttttgcaa ccaaaagcca caaaaaccaa taacaaaata
     8821 aaaattttat tcattttcag ttttttttaa acattacgat atgctcacca acatcgggta
     8881 tttcaaaaat cttgcctgtt ttctgatagc ccattttttc ataaaaccct actgcttcta
     8941 ccctagcgtt aaaccaaatt aaatcaacct tttgggtatt caaaaacgct tcactataaa
     9001 ggatgagcat tttgccaaaa cctgattttt gatactgttc caaaactgcc attcctcgaa
     9061 tttgatattg cttttgttcc ttaaaaacaa ggtttttcgt taggaacaat gatataatac
     9121 ctaccaattc ttcattcaaa taaagaccaa aatgatgagt agctattaaa tcatcaccat
     9181 caaagcggca actttcaata ggttttcctg ctcgaagaac aggatgtcta accgaaaaag
     9241 tttctaaata tggtatttct tttataatag tcgtcataat ataaaaataa tgaatttata
     9301 atgatttaat tttaaaatgg ttataaataa aatctgtttt tttcaaaaaa aaacttgcaa
     9361 tgaaacgaac taattgcata tatttgcacc gttgtaatgc gaaagtagct cagttggtag
     9421 agctccagcc ttccaagctg gttgtcgcga gttcgagcct cgtctttcgc tctataaaac
     9481 ctcaaacgaa agtttggggt tttttattca aagaagcaaa tttggttttt acaaaaattt
     9541 ggataaaccc tgattctttt ttacatttac accattctaa tgcgaaagta gctcagttgg
     9601 tagagctcca gccttccaag ctggttgtcg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnagttcgag cctcgtcttt cgctctaaaa acctcaaacg aaagtttggg
     9781 gttttttata tataagttat ggcgagaagt ttatcccgag cgaagcaatt cgggaagcct
     9841 cgtctttcgc tctaaaaacc tcaaacgaaa gtttcttttt ttatttcaaa aaactcaggt
     9901 cagattcgct atccatttca accggaattt ggttttgtct aaaaagtcga gctttcaaac
     9961 cacctttcaa aaccactttt tctcctttta cctctaacca gcttccttct ctcaatccta
    10021 aaactgggat agtattaaac gaatgaaact ctgcaatcct tgtttcacgg gtttcgccca
    10081 tatgtttaga ttgcatatca gcatccagat aatgaggatt caaattgaaa ggaatcaagc
    10141 ccaatgtttt aaaacttggc ggataaacaa ttggcatatc attggtagtt tgcattgtca
    10201 acccacaaat attgctacca gcactggttc ctaaataagg agttccgttt ttgacagctt
    10261 cggcaagtgc aatcatacaa tcctttttat acaattgagt gactaataaa aaagtattcc
    10321 cgccaccagt aaaaatccct tcagcctttt gaatagcatc cgaataatca tcaacctcgt
    10381 gaatgccttt tatttctttt ccaattttag cgaaagccga agcaacttta aacgcatatt
    10441 cctcatgtga aatgccactt ggacgagcaa aagggataaa caaaatcgtt ttacaatact
    10501 gaaaatgttt ttctaattcg ggtaataaat actctaaata ttcacctccg tggagtgttg
    10561 atgtactggc tataattaaa tttttcattg atgttttagc aaaaaatgtt tgggataaag
    10621 gtatgaaatc gcactactaa tattgcagtt caagaatttt aattaacatt ttcttaccaa
    10681 gtccttagta tttaatttgg aatacattag gataatttta catcattaaa taattgattc
    10741 acccattaat gaaattaggc atttgcatgt tttttgtttt gattgccaat actgttttgg
    10801 ctcaagaatc accaagaaaa atattaaatg gtaaaatcaa tgccaataca atggatcttg
    10861 aaggtattta tgtcatcaat ttaaaaactg aaaaatcaac cattaccgaa aaagagggtt
    10921 atttttcaat atcaaccatg ccgggcgaca cgctcttatt ttcggctatt caactaaaag
    10981 aaaagagaat tgcattggag tcgaaagatt ttaaagaaga attacttcaa gtaaaaatgg
    11041 aaaccaacat aactacatta aaagaagttg tagtaaaaag atacgacaat atcaatgctg
    11101 tttcattagg gatatcacca agtggaatta aacatttaac tcaagcagaa aggcatttga
    11161 aaactgcaag tgaccttgac ccaacagcaa atgcaggctc tatgacgggt ggttctatag
    11221 gactagaccc ccttctgaat ctaatatctg gacgcacagc aatgctaaaa aaagaacttg
    11281 tagtagaagg caaattatct tttattaatc aaatggaaaa catgtttaat gaagattatt
    11341 tcaaaaaaac attgaaaatt ccagcggaat acatcaaagg atttctatac tacattgttg
    11401 ataacgatag atttacaaat attctaaaat ccaaaaacaa aactcaaata gaatttgcca
    11461 tggtttcttt agcagaaaaa tacaaccaaa tcctattgaa tgaaaagtaa aatcccttta
    11521 cttgttatgc ttttattcag tccgattatt ttggggcaaa ctgctgccga aaaaataatt
    11581 cacggaaaaa taattatcga atcacaccct atttctggag ttactatcat taatttggta
    11641 aacgaaaaga gcacagtgag tgatagtaat ggcgaatttt atattttggc gaaagccgat
    11701 gatttattag ttttcacttc ggtgaattta gaataccacc gcaagcaaat cgaagaagaa
    11761 gatttgaaac aagatattat aacaataaaa atgactgcca aaatcaccga gcttgatgaa
    11821 gtaatcatca acaaacaccc agaaatcaat gcagttgcct tggggatttc gccagcagga
    11881 ataaaaaaat atactccagc tgaaagaaga ttgaaaactg cgggcgaatt caagcccatt
    11941 caattacttg gattactcgg cggatctatg ccattagacc ctttaattaa ctctatttca
    12001 ggaagaacag caatgctaaa aaaagaactt gaagtagaga aaaaagagtt gttattagtg
    12061 caaattgatg ctctttatga taatgaatat tttgtacaaa aactaaaaat cccagaggat
    12121 tacattaacg gattcaaaca ttatatcatt gaaaacgaaa aatttactgc agttttaaaa
    12181 tccaaaaata aaaaaatggg aggttttttg atgatagatt tggcaagaga atacaaccaa
    12241 attatattga atgaaaagta aaataggttt cagtttaatt aatttttaat tacactacat
    12301 ctttgtaact ttgcaactta aataaataga ttatgtttgg aataggcggc ggcgaattaa
    12361 tttttatact gtttatagtt ttaatgcttt ttggtgctga caaagtgccc gaaatggcac
    12421 gaaccatggg aaaagccatg gcgcaattaa aaaatgccac aaacgacatc aaaaatgaaa
    12481 ttcaaaaagg agccgaagaa aatggtttcg accaaaaaat gctagacaat ttgactggca
    12541 atatcacttc cgaaatcaat aaaacaaaag ccagtttact tggcgattca aatcctttaa
    12601 aaggaatttc ggataatttt tcatcagaag ttgacaaagt caaaagtagt atcgtaaatg
    12661 aaacttcgac tcagactgtg tcagaatctg aaaacacaaa agaaaattca atctctgaaa
    12721 caactccaaa aactaatgag attgaagaaa taactgggcc aataaaacgc accaaataat
    12781 gctggacaag atacaaactc tcgataccaa actatttatt tatctcaatg gtttaggctc
    12841 agaaaacttc gacggacttt ggctaattat caccaaacaa atctattgga ctccactttt
    12901 cttactcctg ttttatatta ttttcaagga attaggaacc aaacaaagcc tatatttgct
    12961 tttgtttgtg gcggttttac ttgttttcac cgatcaaatc accaatttgt tcaaaaacgg
    13021 atttcaaaga ctacgccctt gcaataatcc cgagataaat actataataa gaatcgttca
    13081 atcccgaaaa tcatttagtt ttttctctgg ccatgcagcc aacacaatgg cggttgctac
    13141 atttttgtac ctcattttca agaaagattt caaatacttt ggcttgctgt ttctatggcc
    13201 attgatcttc gcttatagcc gaatttatct cggattacat tatccaatag acataatttc
    13261 cggctatttc tgtggagcaa tattgggtta tttgatgttc aaattatata gaatagcaca
    13321 aaaaagaaat ttttcggtta aattaaagaa ataaaacagt ttcaagtaat tacaaaaccc
    13381 gagaaaccgt caaaccatca cgaatgggca ataaaactgt ttctactcta gaatcgtttt
    13441 tcaacaaaag attatattcc agcaatattt tagtactgat atcttttgga ctcaagggtt
    13501 caagcacttt tccgctccac aacacattat ccgaaagaat aatgccgcct tggttcattt
    13561 ttggcacaat caattcaaaa taattgatgt aattttcctt atcagcatca ataaaaacta
    13621 agtcaaattt catgtctaaa gttggaataa tatccaaagc ttctccaaga tgttgcacga
    13681 tttgatttcc ccaaggtgat ttgtcaaaat gtttgcgctg aaaatcgacc aattcttctt
    13741 tgatgtcaat cgtgtgaagc gtaccatttt cctgcattcc ttcgcacaaa cacaaagccg
    13801 aataacccgt ataagtacca atttcgagaa tatttacagg acgaattaat ttggacaaca
    13861 tactcaaaac gcgtccttga aaatgaccac tcaacattct tggcaacaac actttctgat
    13921 aggtttcttt gtttaaggct gctaataatt cgggttcttt ttcagaatgt tgctcgatat
    13981 aattttctaa atcttgggaa atgaagtgca ttattttaat ttttgtcaaa gttatcaaat
    14041 tatcaaaaca acacacgagc aattattcga attgatgaaa taaatcaaca aatagtcctt
    14101 aaatttgcag catgcaaatc gagaaaaaag acatacgagc attatcaaaa gaacaattac
    14161 gagaattttt tgttgccaat aatgataaag cgtttcgtgg aaatcaagta tatgaatggt
    14221 tgtggagcaa aggcgcacat agttttgagg atatgactaa tgtatcaaaa actactcgcg
    14281 aaatgctaga aaaccacttt gtaatcaatc acatcaaagt agataaaatg caacgaagtg
    14341 aagacggaac ggtcaaaaat gcggttcgtt tgcacgatgg tttggtagta gaaagtgttt
    14401 tgattcccac caatactaga acgacagctt gtgtttctag ccaagtgggt tgcagtttgg
    14461 attgtaattt ttgcgccaca gcaagactga aaagaatgcg aaatctagaa ccagccgaaa
    14521 tttacgacca agtaatagcc attgataaag aaagtcgttt gtattacaac catcctttat
    14581 cgaatattgt ttttatggga atgggcgaac cgcttatgaa ttacaacaat gtaatgaaag
    14641 ccatcgaaat gattacatcc aacgaaggat tgggaatgtc tccaaaacga attacagttt
    14701 cgacttctgg tgtgccaaaa atgattaaaa aaatggccga tgacgaggta aaattcaatt
    14761 tagccgtttc tttacattca gcaattgatg aaattcgttc tagaattatg cctttcagcg
    14821 aaaattttcc tttggtcgat ttaagagaag cattagaata ttggtaccga aaaaccaaaa
    14881 gcaaagtttc gtatgaatat gtgatttgga aagggattaa cgacaataaa gcctcggttg
    14941 acgcactagt aaaattctgc aaatatgtgc cttgcaaagt caatttaatc gaatacaacc
    15001 cgattgatga tggcgaattt caacaagctt cggaagagtc aatcaatgct tacatcaaag
    15061 cgctcgaagc tactggaatt gtggtaaaag tgcgaagaag tcgcggaaaa gacattgatg
    15121 cagcttgcgg acaattagct aataaagaag cataaaaaga gcttatattg tagatttttt
    15181 ttaaataaaa accctgttcc aaatttggaa cagggttttt attttaaaaa atactgtttc
    15241 tcttattctt gaattttatt gttcagcata gctttgaact tcatgaagat aaagaacaga
    15301 ccaattagtg ctaataccca tacataatca tcaatgggtg ctggggtgtc accactttct
    15361 aaagtgcctg ttgtatcatc agctgtaggc tgtgcaaaca ttacaaaggt ggaacacaag
    15421 taaaaagcag ttatataata ttttaataaa ttatttttca ttttttgtta tttttaaggt
    15481 ttacgatttc gtctattaga gtgtgttaga atactaattt tctactttct tacttactac
    15541 tttgttatct tctgaagtta cttttacaat cagaacttgg tgaattgctt tcaaattgtt
    15601 gattactgca gcatttgctt tcacattatt ttgctctgca attaatctac cttgaatatc
    15661 ataaaccttg atgttattga tagcttttgc agcagagttc acataaatca ctccattatt
    15721 tttgtacaca ataacactgt tgtcgtttgt aacagaagca tcaagtttta atgttttttg
    15781 gtatttcaaa ctaaatcttg cacttgcaga tccagttgct gctgtaaaag tgtaagatga
    15841 tgttgttaaa tcagtttctg ttcctgtaat agcatccgta agaataacac tttgacctgc
    15901 tgcaaaaaca ccttcggaat ggtcaatcgc aatggtataa tcgccagcaa cagctgtttt
    15961 gaagttcaaa gaaactacat cagaagcatc gaatgcagga cggccttgaa tggtatattc
    16021 ttcgttgttg atattggaag tcaaggcaag cggactgtca ttgatatatt ttccatcaaa
    16081 agcatcaaca cctggagtgg cgccatcaaa ataagttacc gccatttgag agaaatcacc
    16141 agccgcattg gtcgcattta accatacttt atcagctgct gcaatttgtt tcgttttaaa
    16201 gaattgacca gcaacattac cccatctttg agaattttta aacaatatac tagtctgtcc
    16261 agtttttgct tctacaaaga aaccttggcc tatttgaagg attgaagcag gatttactga
    16321 taatgaatta ccatttgtaa catatgttcc tgctgttgga gaagtaggaa tataagtgca
    16381 atatgctgtg ccaacaccat tggtttttct ccaaaaatac aaggttgttt gaattttagc
    16441 tgtattttca gttacgaaag tgtacaagtt tattggagaa ggataaggat tacctacaag
    16501 attaaaacgc aatcctacag ctccaccatc cactaatggg actgttatat cgccattgtt
    16561 tggaactcct ataaactgtc ctgggaaagc caaagtagca gttccagcat cataaccact
    16621 agttaaatct gtgttaggca tacgaattaa ataaccagaa cctgctgcaa aattagttac
    16681 tgcaggattc gcaattacac tgaaggcacc atatacacca gccgtattat aagtagtatt
    16741 aaagttgtaa aatcgacttg gagtttgtga agtcaaagga gaaaaagccg ccaagttctg
    16801 acctgccaca ggtgaagacc atatcgtgta atctaaacga tacaaggcat tgctattgcg
    16861 gtttaccacc acgtttcctg tatttgtcgt attgtttgcc tgaatcaggt tgccgttgtt
    16921 ttggataaca aaaccatttg tacccgcatt atttaccact tcattttgta cggttatgat
    16981 agttcctgaa tttattgtca gagaacctga atttaccgtt agttttttgg cgttaaatga
    17041 gccatttgtt gtagtattat aattaccttc gatgacagcc tctgttgaag cggttggaac
    17101 accatcagac caagaagttc cattccaagt cgttattaga attaatggtg gagaaattgc
    17161 gataacagtt gttttgtttg tagctgcatc aaacgtattt gaaattattt ttcc
//
LOCUS       DAOSNN010000022        16695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3479_length_16695_cov_4.295974, whole genome shotgun sequence.
ACCESSION   DAOSNN010000022 DAOSNN010000000
VERSION     DAOSNN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16695
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3479_length_16695_cov_4.295974"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1166
                     /locus_tag="PLQ70_02245"
     CDS             297..1166
                     /locus_tag="PLQ70_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HQE33463.1"
                     /translation="MASGFFALLDDIAAIMDDVAIMSKVAAKKTAGILGDDLAVNAEK
                     ASGFISSREIPVLLAIAKGSFINKLIILPIAFLLSAFFPLAISIILVLGGLFLAYEGA
                     EKIYEYFFPHKHISTEIKIQEYTEEEILALEKDKIKSAIVTDFILSVEIVIIALGTVI
                     GQPISSQIVVVSIIAILATIGVYGIVALIVRMDELGYKLIGLNSKERSINKFIGNILV
                     QALPKVIKSLAVIGTIALILVAGGIFAHNIDFLHHLFNQWPSTVREFSFGLVIGFLVF
                     GIVTLIKLLFGKK"
     gene            1349..2026
                     /locus_tag="PLQ70_02250"
     CDS             1349..2026
                     /locus_tag="PLQ70_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HQE33464.1"
                     /translation="MRTNSRLFALLFIGCSIILKGQSQELNNNSNKKTEWIIADLLRQ
                     KSNTIGISGNPKIVSSPFGDAVSFNGTDDAIFLNELPLQSLQEFTVEMLFKPELNGQF
                     EQRILHIGESKGDRMLLEIRALENNWYFDGYAASGTNKKTLIDEKLIHPLEKWYHVAF
                     VVTSKSRATFVDGKQELREDFPFSPIQTGQTSIGVRMSKINWFKGAIYKIRIIPKEVN
                     PKDFIQS"
     gene            2182..3870
                     /locus_tag="PLQ70_02255"
     CDS             2182..3870
                     /locus_tag="PLQ70_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HQE33465.1"
                     /translation="MKTNPINLLCIALTVVVTSWSNNSLAQNSKANETYQGIEFKMSK
                     VKEPIIPKNTVNIKDFGAVNGGTVLNTKAFADAIAAVSKKGGGKVVIPAGIWLTGPII
                     LKSNIELHAETGALIKFSTDKSLYPLVETSFEGLNTWRCISPIYGKNLENIAFTGKGV
                     WDGSGEVWRQVKKSKLTESQWKKFVASGGVLNDKKDSWYPSETFMKASIGADQNVRLD
                     LKTKEDFEAIHDFLRPVLVSIQNSKRVLFDGPVFQNSPAWNIHPLLIEDLIIRNLTVR
                     NPWFSQNGDGLDVESCKNVLVENSTFDVGDDAICIKSGKDKDGRDRGVPCENVIVRNN
                     IVYHGHGGVTVGSEMSGGVKNLHVSNCSFMGTDVGLRFKSNRGRGGVVENIFISDIFM
                     TDIPSQAISFDLYYGGKSIAETLAEGGNTVSTKFVPVDEKTPQFKNISIKNITILGAY
                     QAVFLQGLPEMNLENIEITNLIGTADKGFSIIDANGIKLSNIKLDIKESTIFDIYNGK
                     NVSLKNIDFNSTSPKAVNINGAACEKIELVSSPKLDFSQKTTIGDTVSKGAVKL"
     gene            3888..5072
                     /gene="pelA"
                     /locus_tag="PLQ70_02260"
     CDS             3888..5072
                     /gene="pelA"
                     /locus_tag="PLQ70_02260"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HQE33466.1"
                     /translation="MQSFSINYRILAFILAVFSSVLTNAQSNAIINTKPFQDGVNHWY
                     HIKDETNIVNPVPNQPQYPESEYQKIADNILYFQRDNGGWPKNYDMRAVLTQDQINDV
                     VKTKSVLHTTFDNSTTYTHIDYLAQVYTLTKNDKYKVGCLKGIDFILKAQFSNGGWPQ
                     YFPLEKGYSRHITFNDGAYIGVMNLLKKIINKNPNYEFIDAKTQRKVSEGYQKGIDCI
                     LKMQIVDQGKLTAWCQQHDEITLLPAWARKFEPPSICNGESAEIVLFLMDIKNPDEKI
                     IQSIQGAVKWFSDSKIYNTRVETFQAPEFESKYKKVTDDCRVVIDTTAPPIWTRYYEL
                     ETEKPLFCDRDSKYLYSLAEVSRERRSGYAWYTYNPAKVLKKYPEWQKKWTPNEDALK
                     QK"
     gene            5244..6131
                     /locus_tag="PLQ70_02265"
     CDS             5244..6131
                     /locus_tag="PLQ70_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HQE33467.1"
                     /translation="MPKNKYKIAVIQLNLNDVAENNLKKCLSWVRDAASQGAEVISLP
                     ELYSSHYFCQSEDVDNFALAEPLYSTSFIAFSALAKELGVVIIVPFFEKRMAGIYHNS
                     AYIIDTDGSEAGLYRKMHIPDDPAFYEKFYFTPGDLGFKNNPTKKGNIGTLICWDQWY
                     PEAARITALKGAEVLFYPTAIGWHPLEKEQYGENQHGAWMNVMKGHAVANGVYVAAAN
                     RIGLEQYIEGTAGIQFWGSSFIAGPQGEILAQASHDKEEILIAEVDLDLQENVRQNWP
                     FFRDRRIDAFGDITKRAID"
     gene            6135..7175
                     /locus_tag="PLQ70_02270"
     CDS             6135..7175
                     /locus_tag="PLQ70_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HQE33468.1"
                     /translation="MSASNRRFPAEWEKQSGILLCFPHNGNDWPGKYEAIQWTFVEFI
                     KKVATFEKVFLVVADEKLKLKVSEILETATVQMSNVSFIIHKTNRSWMRDSGPIIVQN
                     GTEREALNFNFNGWAKYKNINLDKHVPAKVSEFLNLPLTQVVYNGKPVIVEGGAIDSN
                     GKGTLLTSEECLMHPDIQVRNPGFTKADYEAVFKEYLGITNVIWLGDGVEGDDTHGHI
                     DDLCRFVNEDTIITIVEKDPKDNNYKPLKDNLKRLQNAKLENGKSPKIVELPMPKRID
                     FDGLRLPASYANFLILNNCVLVPTFNDSNDRIALNIIADCFPNREIIGINAIDLIWGF
                     GTLHCLSQQIPE"
     gene            complement(7255..9249)
                     /locus_tag="PLQ70_02275"
     CDS             complement(7255..9249)
                     /locus_tag="PLQ70_02275"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQE33469.1"
                     /translation="MKNISFKVLVQFVFLIAVSTVWSQQVSSAKQVSKYNYQDAFGPF
                     FYTKNGTNTRSASGQPGFEYWQNRADYQLSAKLDEANNEISGTSSISYTNNSPDKLSF
                     LWLTLDQNIFKDDSRGNAVIPITGSRNGAQGQIFDGGFKIKSVKIIVSNKGVATEKEI
                     KYLITDTRMQVFLPQELNSKGGNVKIKIDFSFIIPNEGSDRMGILGTSNGKIFSIAQW
                     YPRMCVYDDIVGWNTHPYQGASEFYLEYGDFDVNITVPSHHFVVCSGELINPKEVYSI
                     EQQKRWEQASNSDQTVIIRSADELKKNSNAVSTSTKTWHFKIKNSRDVAWASSASFIL
                     DAARINLPSGKKSIAISAYPIESNGKGAWSRSTEYTKTSIENYSKRWFEYPYPAAINV
                     AGIPGGMEYPGIVFCEYKDKGKDLWGVTDHEFGHIWFPMIVGSNERLFAWMDEGLNTF
                     INSISEEDFNQGEYDEKPLDMHVFSERFTNPNLETLMASPDNMKEINIGTLGYRKPGA
                     ALVILREHILGPERFDFALRTYVERWAYKHPTPDDFFRTIENAAGEDLSWFWRGWFVN
                     NWRLDQGINSVSYVNGNPQLGAFIMIENFEKMAMPVIMDIKTKSGKVSRLKLPVEIWQ
                     KNVNWTFKYNSTEEIESIVIDPDHVFPDSNESNNTWKSVK"
     gene            9412..10275
                     /locus_tag="PLQ70_02280"
     CDS             9412..10275
                     /locus_tag="PLQ70_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HQE33470.1"
                     /translation="MATILTTTISNANLTVQIKHMGAELFSLKNNQNTEYIWEGNPAF
                     WGKHSPILFPIVGTLKNNTYNYNQKQFQLSRHGFAREMEFELVEKSEESATFSLISTL
                     ETKKVYPFDFELQICYSLDENKLNIDYKVINKSETTLPFAIGAHPAFALPGNFEEYSL
                     EFQQAETLKYYLLAEGLISNNSNEIQLDNKKLGLNYQWFEKDALVFKTLESKSITILK
                     NEKSILKVNFNDFPNLGIWTVQNAPFLCIEPWFGYSDTLNEYDDFLKKEGIQLLRKNE
                     TFKSNYSVEIL"
     gene            10279..10830
                     /locus_tag="PLQ70_02285"
     CDS             10279..10830
                     /locus_tag="PLQ70_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE33471.1"
                     /translation="MINLIFNPFPNLETERLLLRRVVNKDVEQIFALRSDKEVMKYIP
                     RPLVKTKDDALAHITMIDEKIDTNEGINWAITLKNNDKLIGIIGLYRIQPEHFRAEIG
                     YMLLPEYHGKGIVSEAINEVVNYGFDAIKLHSIEAIIDPKNFASEKVLQKNGFVKEAH
                     LKENEYYEGRFLDTVIYSIINNM"
     gene            10911..11468
                     /locus_tag="PLQ70_02290"
     CDS             10911..11468
                     /locus_tag="PLQ70_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HQE33472.1"
                     /translation="MKKIFIFTLVFFSICSHSQTYIKANAVTALLLVPNIGVETSIGK
                     KFTFQADVMASFWESFNGHHPMKFYSLVTEVRYHFQEKYNGFYFGGHAGADRYQLQKW
                     NYWGTNEYEDGFGYKLGATIGYEKKLNDRWLLDFFVGGGWHQGYYKGYYNDGTPGRYE
                     NVKNWNRSGDWLPYRGGIMVSYRLN"
     gene            complement(11465..11695)
                     /locus_tag="PLQ70_02295"
     CDS             complement(11465..11695)
                     /locus_tag="PLQ70_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HQE33473.1"
                     /translation="MEKQQSKDPLHGITLQRILEQLIDYYGFDTLGELIKIKCFTDKP
                     SIKSSLTFLRKTDWARKKVEDLYVRTIPKFKI"
     gene            11766..12218
                     /gene="smpB"
                     /locus_tag="PLQ70_02300"
     CDS             11766..12218
                     /gene="smpB"
                     /locus_tag="PLQ70_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQE33474.1"
                     /translation="MLKTINILNKRARFDYEIIEKFTAGIVLAGTEIKSIRLGKANIT
                     ESFCEFNGTELFAINTYIEEYAFGNQFNHKARSERKLLLNKRELKGLARSVQAKGLTI
                     VPLKLFTNEKGMAKLEIGLCKGKKIYDKRESLKEQDTKRDLDRIKKSF"
     gene            12306..12434
                     /locus_tag="PLQ70_02305"
     CDS             12306..12434
                     /locus_tag="PLQ70_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33475.1"
                     /translation="MKKFLIGLSLIFLASCSSGTAIVSSWRDPDTSTANTEFKKYW"
     gene            12428..12946
                     /locus_tag="PLQ70_02310"
     CDS             12428..12946
                     /locus_tag="PLQ70_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33476.1"
                     /translation="MVVALVKNEATRRVTENRIASIGPKFHSSYAILNGTSLNLTKDQ
                     KLKILKDENYDAVVTMRLVDTVKETNYVPGTNTSMYYGGYGGMYGGYGAYGGFGGWYG
                     MYSPIYYDPGYYQESTSYLIETNVFSLKSDKLVWTGTTKSSNGSDVGLLVDSIIATVM
                     QEMKKDGFIPKK"
     gene            13064..14113
                     /locus_tag="PLQ70_02315"
     CDS             13064..14113
                     /locus_tag="PLQ70_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQE33477.1"
                     /translation="MKTLLKNAREQKGLKTREVAQILGIDQALISKFENGTRKPTKEQ
                     VLKLASLLGIDFETLMVAWLKEKILYEIGQDEFALKAMNMVREEIENSYTSAKKSLSK
                     NLSAILGEIDILKSKLDQFRKFDSYRIAQALELEYTFESNRIEGNTMTLRETDLVVNE
                     GLTISGKSMREHLEVINHQEAIAYIKHLMDKNTPLKEREVLSIHNLILRGINPEDAGR
                     YRQVQVMIKGSSFMPPQPFLVAKEMEDFFIWYETHKNTLHPIVLAAELHERLVTIHPF
                     IDGNGRTSRLVMNLILLQNGYVIANIKGDYDSRIQYYNALETAQTKNNKEDFLFFIAQ
                     IEKESLERYLEIIGQ"
     gene            complement(14149..>16695)
                     /locus_tag="PLQ70_02320"
     CDS             complement(14149..>16695)
                     /locus_tag="PLQ70_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HQE33478.1"
                     /translation="GDEAAKRQEILKNIENNDIDYYLNTFSKFTDTAWLTNTSVQELE
                     KYRDISKQYKQRYGELMFDFMLASRKADIDGMFKYLNDLNENSYNNEKFKILTANLYS
                     KLKNDKAKSITLLEEISKEKSINEVENLLINYYNDINRKEEAKNIITNRIAKFPCLNS
                     FRNDYISILTDENQYQKALELINENLSYFPYSFTNLEKKANIYSLMKKDKEAEKYIRL
                     SLEHNSENGNLRKQLYDITKTPDEIEEVATKNCYKLANERKNSKLKSDYGVVVLLDEY
                     IVNILPEGGRKSKCTYLYEITSESGIEEMKEYSLDLSNTILKSEIIKSDGTLVPAEKG
                     DDTLVFSNLKIGDVIYIQYEVQENTTGRFYKDFNISCYFDSKYPSVESIFGLIHEPNV
                     NYSTDYSNGLVESTTKKIGNKIGTVWKRNNIPAIPLLENYSINFNDLTNKVRVSTIKS
                     WKDISNWYADLVKKNLKVDKTTKTVFDQIFPEGSQIFSQEEIAKKIYSYIETNINYSS
                     LDFRQSGYVPQKPSKTITTRLGDCKDVSTLFVVLAQQAGLKANLVLVLTNDNSSNSLK
                     LPSRDFNHCIVRVTIDNKEVFLELTDKFLPFKALPITLYKAQALVISFDKAINENSSL
                     INIPFENAIQNSVKTYSVVTIDDKLKSFVNTHTIQGANKSYYNELFSSSTTDDVRSKD
                     FEKNFNNQLKKVVSLQSVKLVSNEVFDKEITFESKFSISESLQKVGSLEITEIPFIDN
                     VYTRDIIATDTRKFDIQYIDYENSNRYDSEVILNIPSDKTFSEIPENKSFNFINHNYS
                     ITYQLINNNSLKVNRKVTIPWDNISTTQYVEFKKFVEKIVETEEQIVGFK"
BASE COUNT         5738 a         2690 c         2755 g         5512 t
ORIGIN      
        1 gcccgaagaa atgatttttg ttggcgattc tggaattgat atgcaaacag caactaatgc
       61 taatatattg gcagttggcg ttttatgggg ctatagacca gaatcagaat tatttgccac
      121 tggagcaaaa tatattttaa accatccttt agatttaatt caaatattaa aataaggtaa
      181 aaaacggaaa atattaagca tttaaactac taaaattcca tactattttc caccaaaaaa
      241 atagtatttt cgcaccatca aaaattcaaa aatcaatcaa tttctaaaac aattatatgg
      301 catcaggttt tttcgcactt ttagatgata ttgcagccat tatggatgat gtggcaataa
      361 tgagtaaggt tgcagccaaa aaaacggctg gaattttggg tgatgatttg gctgtaaatg
      421 ccgaaaaagc atctggattt atttcctcaa gagaaattcc agtattgtta gccattgcca
      481 aaggttcatt tattaataaa ttaatcattt tgcccattgc cttccttctg agtgcttttt
      541 ttcctttggc aattagtatc attttagtat tgggtggact tttcttggcc tacgaaggag
      601 ccgaaaaaat atacgaatat ttctttcctc ataaacacat ttcaaccgaa ataaagattc
      661 aagaatacac cgaagaagaa attttggcac tcgaaaaaga caaaataaaa tcggctatag
      721 tgactgattt tattttatca gtagaaatag ttatcattgc tcttggcaca gtaattggac
      781 aacccattag ctcgcaaatc gtggttgttt ctattattgc catactcgca accattggtg
      841 tttacggtat tgtagctcta attgttagaa tggacgaatt gggatacaaa cttattggac
      901 tgaattctaa agagcgtagt ataaacaaat ttataggaaa catacttgtt caagccttac
      961 caaaagtaat caaaagttta gccgtaattg gcactattgc gctgatttta gtagctggtg
     1021 gtatctttgc acacaacatc gattttctgc atcatttatt caatcaatgg ccttcaacag
     1081 ttagagagtt tagctttgga cttgtgattg gattcctagt ttttggaatt gtaaccctaa
     1141 tcaaactgct ttttggaaaa aaataaatct acataaaaat cataatctac aactctaaat
     1201 acagcagttg actaagaatt ttacactatt ttgaatacta aatttactaa ttaataattt
     1261 atctaataat aattggtaaa tactcatttt ttaaatactt ttgagtaatc gattgcatta
     1321 tttaaaattc aaatggtatg gccaataaat gagaacaaat tcacgactat ttgcattgct
     1381 ttttataggt tgttccatca ttttgaaggg acaaagtcaa gaattaaata acaattcaaa
     1441 caaaaaaacc gaatggataa tcgctgattt gcttcgacaa aaatcaaata ctatcggaat
     1501 tagtggcaat cctaaaattg tttcttcccc ttttggagat gccgtttctt ttaacggaac
     1561 agacgatgcc atttttctga atgaacttcc gctgcaatca cttcaagaat ttacggtaga
     1621 aatgctattc aaacctgaac ttaacggtca atttgaacaa agaattctac atattggcga
     1681 aagcaaaggc gatcgaatgc tactcgaaat tagagcttta gaaaacaatt ggtattttga
     1741 tggttatgca gcttctggaa ctaataaaaa aacactaatc gacgaaaaat taattcatcc
     1801 cttagaaaaa tggtatcatg ttgcctttgt tgtaacctca aaaagtcgcg ccacttttgt
     1861 tgatggcaaa caagaattac gcgaagattt ccctttctca ccgattcaaa caggacaaac
     1921 ctctattgga gttcgaatga gtaaaataaa ctggtttaaa ggcgcaattt ataaaattcg
     1981 aattatccca aaagaagtaa atccaaaaga ttttattcaa tcttaaaaat gtaaaatatc
     2041 aaaagtaata acaaaatcaa gattcaatca acttgaagtt aaatatcaaa aacggtctgg
     2101 ttattgttaa ggaactaata aatttaaaat agcaacaaat tcaaattaaa agtaaattat
     2161 aatattaatc aaaaaataag aatgaaaaca aacccaatta acttattatg catcgccttg
     2221 acagtagttg taacaagctg gtctaataac agtttagctc aaaattcaaa ggctaacgaa
     2281 acttaccaag gcattgaatt taaaatgtct aaagttaaag aacccataat acccaaaaat
     2341 actgtcaata tcaaagattt tggtgctgta aatggtggaa cagttttaaa cacaaaagct
     2401 tttgccgatg ccatcgcagc cgtatctaaa aaaggtggtg gaaaagtagt aatccccgca
     2461 ggaatttggc ttacaggtcc cattattttg aaaagcaaca tcgaattgca cgctgaaact
     2521 ggtgctttaa taaaattttc aaccgataaa agtttgtatc cacttgttga aaccagtttt
     2581 gaaggactaa atacctggcg ttgcatctct cctatttacg ggaaaaatct agaaaatata
     2641 gcttttactg gaaaaggtgt ttgggacggt tcaggcgaag tttggagaca agtcaaaaaa
     2701 agcaaactta cagaaagcca atggaaaaaa tttgtggctt ctggaggagt gcttaatgac
     2761 aaaaaagaca gttggtatcc ctcagaaaca tttatgaaag cttctattgg tgcagaccaa
     2821 aatgtgcgtt tggatttaaa aaccaaagaa gattttgaag ccattcacga ttttcttcgt
     2881 ccagtattgg ttagcattca aaacagtaaa agagtcctgt ttgacgggcc agtttttcaa
     2941 aattctccag cttggaatat tcatcctctg ctaattgaag atttaattat tcgaaatcta
     3001 accgttcgta atccttggtt ttcacaaaat ggtgacggtc tagacgtaga atcttgcaaa
     3061 aatgtattgg tcgaaaattc tacttttgac gtgggtgacg atgctatttg tatcaaatca
     3121 ggaaaagaca aagacggtcg tgatcgtgga gttccttgcg aaaatgtaat tgttagaaat
     3181 aatattgttt accacggaca tggtggtgtt actgtaggga gtgaaatgtc tggaggtgta
     3241 aaaaatttac acgtttccaa ttgttctttt atgggaactg acgttggttt gcgtttcaaa
     3301 agtaaccgtg gacgcggtgg tgttgtagaa aatattttta tctctgacat ttttatgacc
     3361 gatattccat cgcaagccat ttctttcgat ctttattatg gaggaaaatc catagccgaa
     3421 actttggccg aaggtggaaa tactgtgagt actaaatttg tacctgtgga tgaaaaaaca
     3481 ccacaattca aaaatatttc tattaaaaac ataaccattt taggtgctta tcaagcggtg
     3541 tttttacaag gtctgcctga aatgaatcta gaaaacatag aaatcactaa cttgattgga
     3601 actgctgaca aaggattttc aattattgat gccaacggaa tcaaattgag caacatcaaa
     3661 ttggatatca aagagagcac tatttttgac atatataatg gtaaaaatgt atccttaaaa
     3721 aacattgatt ttaattcgac ttcgccaaaa gcagtgaaca ttaatggagc agcttgcgag
     3781 aaaattgaat tggtttcatc accaaaattg gatttttctc aaaaaactac cattggagac
     3841 actgtttcta aaggtgctgt aaaattataa cgcataaata aattataatg caaagttttt
     3901 caataaatta tagaattctt gcctttattc tagctgtttt ttcttcagtt ttgacaaatg
     3961 ctcagtctaa tgcaatcatt aacacaaagc catttcaaga cggtgttaat cattggtatc
     4021 acataaaaga cgaaaccaat attgtaaacc cggttcccaa ccagccacaa tatccagaat
     4081 ccgaatacca aaaaatagcg gataacattt tatattttca gcgtgacaat ggtggatggc
     4141 ccaaaaatta tgatatgcga gctgtattaa ctcaagatca aataaacgat gtcgttaaaa
     4201 caaaatcagt cttgcacact acttttgaca attcaaccac ctatacccat atcgattatt
     4261 tagctcaagt ttacacgcta actaaaaacg ataaatataa agtaggttgt ctaaaaggaa
     4321 tcgattttat tctcaaggca caattttcga atggcggatg gccacaatat tttcctttag
     4381 aaaaaggata tagtcgccac attaccttca acgatggagc gtatataggc gttatgaatt
     4441 tgctaaaaaa aataataaat aagaatccta attatgaatt catagatgcc aaaacccaaa
     4501 gaaaagtatc tgaagggtat caaaaaggaa ttgactgcat cctgaaaatg caaattgtcg
     4561 atcaaggaaa actgaccgct tggtgccaac aacatgacga aataacttta ttgccagctt
     4621 gggctagaaa atttgaaccg ccaagtattt gtaatggaga aagtgccgaa atcgttttat
     4681 ttttaatgga cattaaaaac ccagatgaaa aaataatcca atctattcaa ggggctgtaa
     4741 aatggttttc agattcaaaa atttacaata cccgagttga aacttttcaa gctcctgaat
     4801 ttgaatccaa atacaaaaaa gttaccgatg attgtagagt tgtaatcgat acaactgctc
     4861 ccccaatttg gacacgttat tacgaattag aaactgaaaa acctttgttt tgcgaccgcg
     4921 atagcaagta tttatattca ttggccgaag tgagtcgcga aagacgcagc ggatatgctt
     4981 ggtacaccta caaccctgca aaggttttga aaaaatatcc cgaatggcag aaaaaatgga
     5041 ctcctaatga agatgcttta aaacaaaaat agcatttata taaaagcgtg gattatacaa
     5101 aattcacgct ttttagtttt aattatcttc ctttgataac tctaacttta gtatggggct
     5161 atttgaagag acaaaaaatc caatcggtat tccttcttac ttctttataa aataactatt
     5221 tttgcagtca aaatttttct cttatgccaa agaataaata caaaatagcg gtcattcaat
     5281 tgaatctgaa tgatgttgcc gaaaataatt taaaaaaatg tttgtcgtgg gttcgcgatg
     5341 ctgccagtca aggtgccgaa gtgatttcgt tgcccgaatt atacagcagc cactattttt
     5401 gtcaaagtga agacgtagat aattttgcct tggcagaacc tttgtacagc acttcgttta
     5461 ttgcttttag tgctttggca aaagaattgg gcgtggtcat cattgttcct ttcttcgaaa
     5521 aaagaatggc gggaatttac cacaacagcg cctacatcat cgacaccgat ggttcggaag
     5581 cgggattgta tcgtaaaatg cacattccag atgacccggc tttctatgaa aaattctatt
     5641 tcacgccagg tgatttaggt tttaaaaaca atccaactaa aaaaggaaat attggaacac
     5701 tgatttgttg ggatcaatgg tatcccgaag cggctcgtat aactgctttg aaaggtgccg
     5761 aagtgttgtt ttacccaaca gccattggtt ggcatccttt agaaaaagaa caatatggcg
     5821 aaaaccaaca cggtgcctgg atgaacgtaa tgaaaggtca cgctgtcgcc aatggcgttt
     5881 atgtagctgc tgccaataga attggattgg aacaatatat tgaaggaact gcaggaattc
     5941 aattttgggg ttcttcgttt atagctggac cacaaggcga aatcttggca caagcctctc
     6001 acgataaaga agaaatcctg attgccgaag tggatttgga tttacaagaa aatgtgcgcc
     6061 aaaactggcc tttctttaga gacagacgca tcgatgcttt tggagatatt actaaaagag
     6121 caatcgacta atttatgagt gcatctaaca gacgttttcc tgctgaatgg gaaaaacaat
     6181 caggcatttt attgtgtttt ccgcataatg gcaacgattg gcccggaaaa tacgaagcca
     6241 ttcaatggac ttttgtggaa ttcatcaaaa aagtggccac attcgaaaaa gtttttttgg
     6301 ttgtagccga cgaaaaacta aaattgaaag tttcggaaat actcgaaact gcaactgttc
     6361 aaatgagcaa tgtttctttc atcattcata aaaccaatag aagttggatg cgcgattcgg
     6421 ggccaattat tgttcaaaac ggaacggaaa gagaagcctt gaatttcaat tttaacggtt
     6481 gggcaaaata taaaaacatc aatctggaca aacacgtgcc tgcgaaagtg agtgagtttc
     6541 taaatcttcc tcttacgcaa gtcgtgtata acggaaaacc cgtaattgta gaaggcggag
     6601 ccattgacag caacggaaaa ggaacgttac taacatccga agaatgcttg atgcatcctg
     6661 atattcaggt tcggaatcct ggttttacaa aagccgatta tgaagcagtt ttcaaagaat
     6721 atttgggaat taccaatgtg atttggctag gtgacggagt tgaaggcgat gatactcacg
     6781 gtcatatcga cgatttatgc cgatttgtaa atgaggacac catcattacc attgtagaaa
     6841 aagaccctaa agacaacaat tacaaaccct tgaaagacaa tttgaaacgt ttgcaaaacg
     6901 cgaagttgga aaacggaaaa tctcccaaaa ttgtggaatt gccaatgccg aaacgtatcg
     6961 attttgacgg tttgcgcttg ccggctagtt atgctaattt cctgattttg aataattgtg
     7021 ttttggtgcc tactttcaat gattcaaacg atagaattgc cttgaacatc atagccgatt
     7081 gtttccccaa tagagaaatt attggaatca acgccattga tttgatttgg ggttttggaa
     7141 ctttacattg tttgagtcaa caaattccag aataagagtt taacctttta taaaaaagac
     7201 cgcagattcg cagattaaaa tgataatctt cgaatctgcg gtttattttt agtgttattt
     7261 aactgatttc caagtattat tactttcgtt agaatctgga aaaacgtgat ctgggtcaat
     7321 aacaatactt tcaatttctt cggtagaatt gtatttaaaa gtccaattca cgtttttttg
     7381 ccatatttct acgggtaatt tgaggcgtga aacttttccg cttttggttt taatgtccat
     7441 aattacaggc atcgccattt tctcgaaatt ttcaatcata atgaaagcgc ccaattgggg
     7501 attgccgttt acataactta cagaattaat tccttgatcc aaacgccaat tattaacaaa
     7561 ccatcctctc caaaaccaac ttaaatcttc gccagcagca ttctcaatcg ttctaaaaaa
     7621 atcatcaggt gtaggatgtt tataagccca acgctctaca taagtgcgta gagcaaagtc
     7681 aaatcgttca ggccccaaaa tatgctcacg caaaataacc aaggccgcac caggttttct
     7741 atatccaaga gttccgatat taatttcttt catattatca ggtgaagcca tcaatgtttc
     7801 tagatttgga tttgtaaatc tttctgaaaa aacatgcata tccaaaggtt tttcatcgta
     7861 ttctccttgg ttaaaatctt cttcactaat tgaattgata aatgtattta aaccttcgtc
     7921 catccaagcg aataaacgtt cattagaccc cacaatcatt ggaaaccaaa tgtgcccaaa
     7981 ttcgtgatcg gtaacgcccc acaaatcttt ccctttgtct ttatattcac aaaaaactat
     8041 ccctggatac tccataccac caggaattcc tgctacattt atagctgctg gatatggata
     8101 ttcgaaccat ctcttggaat aattttcaat agaagtttta gtatattcag tagatcggct
     8161 ccaagcacct ttaccattac tttcgattgg gtaagcagaa attgctatcg atttttttcc
     8221 acttggtaaa ttaattctag cagcatctaa aataaatgaa gcagacgaag cccaagccac
     8281 atcacgagag tttttaattt taaaatgcca agttttagta gatgtagaaa cagcatttga
     8341 gttttttttt aattcatcag ccgatcgaat tataacagtt tgatcgctat tcgatgcttg
     8401 ttcccaacgc ttttgttgtt caatagaata tacctctttt gggttgatta attcacctga
     8461 acataccaca aaatgatggc ttggcacagt aatattcaca tcaaaatcgc cgtattccaa
     8521 ataaaattca gaggctcctt gatagggatg agtattccat cccacaatat cgtcataaac
     8581 acacattcta ggataccatt gggcaatgct aaaaatcttg ccattggaag ttcccaaaat
     8641 tcccatacga tctgaacctt cattgggaat tataaatgaa aaatcgattt tgattttgac
     8701 atttcctcct tttgaattta attcttgagg aagaaaaacc tgcattctgg tatctgtaat
     8761 taaatattta atttcttttt cagtagcaac acctttgttt gaaactataa tcttaacaga
     8821 tttaatttta aaaccaccat caaatatctg accttgagca ccatttctac tacctgttat
     8881 aggaattaca gcatttcctc gagaatcgtc tttgaaaata ttttgatcta aagtcaacca
     8941 caaaaaagac aatttgtctg gactattatt tgtataagat atactactcg ttcctgaaat
     9001 ttcattattt gcctcatcta attttgcaga taattgataa tcagctctgt tttgccaata
     9061 ttcaaatcca ggttgcccgc ttgctgaacg cgtgtttgta ccgttttttg tatagaaaaa
     9121 tggaccaaaa gcatcttgat aattatattt tgaaacttgc tttgctgaag aaacttgttg
     9181 tgaccataca gtggaaacag caatcaaaaa cacaaattga acgaggactt tgaaagaaat
     9241 atttttcatt tattaaattt tatagtgcaa attaatgaaa aataaacgta gcacttagaa
     9301 acaatactta aatattggtt aaaaatttca tctttagaat tgaatacttt ttattaccta
     9361 aatttaattt tatggaatat atttacagct cagtaaaaaa tattaatcta attggcaact
     9421 attttgacta caacaatttc taatgcgaat ctaacagttc aaataaaaca catgggagct
     9481 gaattatttt ctttaaaaaa caaccaaaat accgaatata tttgggaagg aaatccagcc
     9541 ttttggggaa aacattctcc gattttgttt ccaattgtgg gaactttaaa aaacaatact
     9601 tataactaca accaaaaaca atttcaattg agcagacacg gttttgctag agaaatggaa
     9661 tttgaattag ttgaaaaatc agaagaaagt gctacgtttt ctttaatttc aacattagaa
     9721 actaagaaag tatatccttt tgactttgaa ttgcaaattt gttattcatt ggatgaaaat
     9781 aaattaaaca ttgattataa ggtcattaac aaatctgaaa caacattgcc atttgccatt
     9841 ggagcgcatc ctgcatttgc attgcctgga aattttgaag aatatagtct tgaatttcaa
     9901 caagctgaaa cactaaaata ttatcttctg gcagaaggtt taatttcaaa caactcgaat
     9961 gaaattcagt tagataataa aaaacttggt ttaaattatc agtggttcga aaaagacgct
    10021 ttagtattta aaacattaga atccaaatca ataaccattc taaaaaacga aaaatcgata
    10081 cttaaagtca attttaatga ttttccaaat ttaggtattt ggacggttca aaatgctcct
    10141 tttttatgta tcgaaccgtg gtttggttat tctgatactt taaacgaata tgatgacttt
    10201 ttgaaaaaag aaggcattca gcttttgaga aaaaatgaaa cattcaaatc taattatagt
    10261 gttgaaattt tataaattat gataaaccta attttcaatc cttttccgaa tcttgaaacc
    10321 gaacgtttgc tgctaagacg tgttgtaaac aaagatgtag aacaaatttt tgcacttaga
    10381 tctgataagg aagtgatgaa atacattccg agaccattag taaaaaccaa agatgatgct
    10441 ttggcacata ttacaatgat tgatgaaaaa attgacacta atgaaggaat taattgggct
    10501 ataacattaa aaaacaacga taaactgatt ggaattattg ggctttacag aatacaacct
    10561 gaacattttc gagcagaaat tggctatatg ctacttccag aatatcacgg aaaagggatt
    10621 gtttcagagg cgataaatga agtcgttaat tatggttttg atgcaattaa attgcattca
    10681 attgaagcaa ttattgatcc caaaaatttt gcttcggaaa aagtattgca aaaaaatggg
    10741 tttgtaaagg aagctcattt aaaagaaaat gaatattatg aagggcgatt cttggatact
    10801 gtgatttact cgattataaa taatatgtaa ttttttaaaa cctttaaatc tatttctact
    10861 atatttgccg agactagttt gtaaatgata tactagtaaa ttttattttt atgaaaaaaa
    10921 tcttcatttt tacattagta ttcttttcaa tttgttctca ttctcaaacc tatataaaag
    10981 caaatgctgt aactgcttta ttattagtgc ccaatattgg agtagaaacc agtattggaa
    11041 aaaaattcac ttttcaggct gatgtcatgg cttctttttg ggaatccttt aatggacatc
    11101 acccaatgaa attttattct ttagtaactg aagttcgcta tcattttcag gaaaaataca
    11161 atggctttta ttttggggga cacgctggag ctgacagata tcaactccaa aaatggaact
    11221 attggggtac caatgaatat gaagatggat ttgggtacaa attgggagca acaattggtt
    11281 atgaaaaaaa attaaatgac agatggttgc tggatttctt tgttggagga ggttggcatc
    11341 aaggttatta taaaggttac tataacgacg gaactccagg taggtatgaa aatgtcaaaa
    11401 attggaatcg tagcggagat tggttgcctt accgaggtgg aattatggtt tcttatagat
    11461 taaattagat cttaaattta ggaattgttc ttacatacaa atcttctact ttttttcttg
    11521 cccaatctgt ttttcttaaa aaggttaaac tcgattttat acttggttta tcagtaaaac
    11581 atttaatttt tatcaattca cccaaagtat caaacccata ataatcgatg agttgttcca
    11641 aaattctttg taatgtaatt ccgtgaagcg gatcttttga ttgttgtttt tccatttggc
    11701 aaatttacgg catttttacc aatttgtttg gcgtttcgta catttgtatt tacatttgct
    11761 gacctatgct aaaaacgata aacatactca acaaaagagc ccgatttgat tatgaaataa
    11821 tcgaaaaatt tactgctgga attgttttgg caggtactga aataaaatct attcgattag
    11881 gaaaagccaa tattaccgaa agtttttgcg aatttaatgg tacagaactt tttgctatta
    11941 acacttacat cgaagaatat gcttttggaa atcaattcaa tcacaaagcc agaagtgaac
    12001 gcaaactatt gctaaacaaa cgtgaactca aaggattggc aagaagtgtt caagcaaagg
    12061 gacttacaat cgttcccttg aaattattta ccaatgaaaa agggatggca aaactagaaa
    12121 ttgggctttg caaaggaaag aaaatctatg acaaacgcga atctctaaaa gaacaagaca
    12181 ctaaaagaga tctagataga ataaaaaaat ctttctaaat taatttattg ataaattact
    12241 ttatttcgca atcattttct gatttaattc ctatttttac ctcgaactaa aaataaaaaa
    12301 ataatatgaa aaagttttta attggattat cattaatttt tcttgctagt tgtagcagtg
    12361 gaacagcaat tgttagcagt tggagagacc cagacacatc aacagctaat acagaattca
    12421 aaaaatattg gtagttgctt tagtaaaaaa tgaagctaca agaagagtta ccgaaaatag
    12481 aattgcttct attggaccaa aatttcattc ttcttatgcc attttgaatg gaactagctt
    12541 gaatttgacc aaagatcaaa aattaaaaat ccttaaagat gaaaattatg atgccgtagt
    12601 aacaatgcgt cttgttgaca ctgttaaaga aacgaattat gttcctggca caaatacttc
    12661 tatgtattat ggtggatacg gtggaatgta tggcggttat ggagcttatg gtggttttgg
    12721 cggttggtac ggaatgtatt ctccaatcta ttatgatcca ggatattacc aagaatctac
    12781 ttcgtattta attgaaacca atgtgttttc gttgaaatct gacaaattag tttggactgg
    12841 aacaacaaaa tcatctaatg gttcggatgt tggactttta gttgatagta ttattgcaac
    12901 tgttatgcaa gaaatgaaaa aagatggatt tattccaaaa aaataattaa atctttaaac
    12961 agatttatcc ccaaaaaata atacttaatc ttttttaggt gttatttttt ttatctaata
    13021 aattatgact aattttgtca agacaatttt taacaaattt aaaatgaaaa cgctactcaa
    13081 aaatgcaaga gaacaaaaag ggttaaaaac cagagaagtg gctcaaattt taggaatcga
    13141 tcaagcatta atcagtaagt tcgaaaatgg cacacgaaaa ccaaccaaag agcaagtgct
    13201 taaattagct tcattgttag gaatagattt tgaaacattg atggttgctt ggctcaaaga
    13261 gaaaattcta tatgaaatag gtcaagatga atttgctcta aaagccatga atatggttcg
    13321 tgaagaaata gagaacagct atacaagtgc caaaaaatca ctttctaaaa acttgtcagc
    13381 tattcttgga gaaattgaca ttttgaaatc aaaattagac caatttcgca aatttgatag
    13441 ttatagaata gctcaagcat tagaacttga atatactttt gaaagcaatc gcatcgaagg
    13501 aaacactatg acacttcgcg aaaccgattt agtcgtaaac gaaggtttga ccatttctgg
    13561 aaaaagtatg cgtgaacatc ttgaagttat caatcatcaa gaagctattg cttatatcaa
    13621 acatttgatg gacaaaaaca ctcctctgaa ggagcgcgaa gtattgtcta ttcacaattt
    13681 gattttgcga ggtataaatc cagaagatgc tggtcgttat cgtcaggttc aagtaatgat
    13741 aaaagggagc tcgtttatgc cgcctcaacc ttttttggtt gcaaaggaaa tggaagattt
    13801 ttttatttgg tatgaaactc ataaaaacac tttacatcct atagttttag ctgctgaact
    13861 tcacgaacgt ttggttacta ttcatccatt catagatggg aacggcagaa cttcgcgttt
    13921 ggttatgaat cttattttgc ttcaaaacgg ttatgtaatt gccaatatca aaggtgatta
    13981 tgactctaga atacaatatt ataatgcgct agaaactgct caaaccaaaa acaataaaga
    14041 agattttctt ttcttcattg ctcaaatcga gaaagaaagt ttagaacgtt atcttgaaat
    14101 tattggtcaa taaaaaaatc ccattcgtag taatgaatgg gatagttttt atttaaatcc
    14161 tactatttgt tcttcagttt caacgatttt ttcaacaaat tttttaaatt ctacatactg
    14221 agttgtagaa atattatccc aaggtattgt tactttcctg ttaactttta atgaattatt
    14281 atttattaat tgatatgtta ttgaataatt atgattaatg aaattgaatg atttgttttc
    14341 tggaatttca gagaatgttt tatctgaagg aatatttaaa atcacttctg aatcatatcg
    14401 attactattt tcataatcta tatattgtat atcaaatttt ctagtatcgg tggctattat
    14461 atctcttgtg tatacattat caataaatgg aatttcagta atttctaaac ttcccacttt
    14521 ttgtaagctt tcagaaattg aaaattttga ttcaaaagta atctccttat caaagacttc
    14581 attggagacc aatttaacag attgcaacga aactaccttc tttaattgat tattgaaatt
    14641 cttttcaaaa tcttttgaac gaacatcatc agtagtcgaa gatgaaaata attcgttata
    14701 gtacgattta tttgctcctt gaatagtatg cgtatttaca aacgatttca atttatcatc
    14761 tatggtaact acagaataag ttttaacact attttgaata gcattttcaa aaggaatatt
    14821 aatcaatgat gaattttcgt ttattgcttt gtcaaaagag attacaagag cctgagcttt
    14881 atataaagtt attggcaatg ctttgaaagg taaaaactta tctgtaagtt caagaaatac
    14941 ttctttatta tcgattgtta ctcttacgat acaatgatta aaatctcttg atggcaattt
    15001 taatgaattt gaactattgt cattggttaa aaccaaaact aaattagctt ttaatccagc
    15061 ttgctgtgct aagacaacaa acaaagttga aacatcctta caatctccta atcttgttgt
    15121 aattgttttg gaaggttttt gcggaacata accgctttgt ctaaaatcta aagaactata
    15181 attaatgttc gtttctatgt aggagtaaat ttttttggct atttcttctt gtgaaaaaat
    15241 ttgtgagcct tctggaaaaa tttgatcaaa tacagttttt gtagttttat caactttcaa
    15301 atttttctta accaaatcag cataccagtt tgatatatct ttccacgatt taatcgtact
    15361 tactcttact ttattagtta gatcattaaa atttatagag taattttcta acaaaggaat
    15421 tgctggtata ttatttcgtt tccaaactgt gcctatttta tttcctattt tttttgtagt
    15481 tgattctacc aaaccgttac tataatctgt actataattt acattcggct cgtgaataag
    15541 tccaaaaata gattctacag atggatattt actatcaaaa taacaggaaa tattaaaatc
    15601 tttgtaaaat ctcccagttg tattttcttg cacttcgtat tgaatataaa taacatctcc
    15661 tatttttaaa tttgaaaata caagtgtgtc atcgcccttt tcggctggga ctaatgtacc
    15721 atctgattta ataatttcgg attttaaaat tgtattactc aagtcaagtg aatattcttt
    15781 catttcttct attccgcttt cggatgttat ttcatataaa tatgtgcatt tagactttct
    15841 accaccctct ggcaaaatat tcacaatata ttcgtctaat aaaactacaa ctccataatc
    15901 gctttttagt tttgaatttt ttctttcatt tgctaatttg tagcaatttt tagttgcaac
    15961 ctcttctatt tcatccggtg tttttgtaat gtcgtatagt tgttttctta aatttccatt
    16021 ttctgaattg tgttctaaag acaatctgat atatttttca gcttctttat cttttttcat
    16081 caaactataa atattggcct ttttttctaa attagtaaat gaatagggaa aataacttag
    16141 attttcattt attaattcca gtgctttttg atattggttt tcatcagtta ggatggagat
    16201 ataatcattt ctaaatgaat taagacaagg gaatttggct attctattgg taatgatatt
    16261 tttagcttct tcttttctat ttatatcatt ataataatta atcaacaaat tttctacttc
    16321 gttaatactt ttttctttcg aaatctcttc taataaagtt attgatttcg ccttgtcatt
    16381 cttcaattta gaatacaaat tggctgtaag tatcttaaac ttttcattat tatacgaatt
    16441 ctcatttaaa tcattgagat acttgaacat cccatcaata tcagcttttc ttgaggccaa
    16501 cataaaatca aacatcaact ctccatatct ttgtttatat tgtttggata tatctctata
    16561 tttttctaac tcttgcacag aagtatttgt taaccaagca gtatcagtaa atttggaaaa
    16621 tgtatttaaa taataatcaa tatcgttgtt ttctatattt tttagtattt cttgtctttt
    16681 tgcagcttca tcccc
//
LOCUS       DAOSNN010000023        16517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3535_length_16517_cov_4.122099, whole genome shotgun sequence.
ACCESSION   DAOSNN010000023 DAOSNN010000000
VERSION     DAOSNN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16517
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3535_length_16517_cov_4.122099"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLQ70_02325"
     CDS             <1..278
                     /locus_tag="PLQ70_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQE33479.1"
                     /translation="EATLKGLNKLLLNESKFAEDLEKNWAVVAEAIQTILRREAYPNP
                     YEALKGLTRTNEAITKDSIHAFIQTLEVSAEIKAELMQITPSNFLGI"
     gene            309..1079
                     /locus_tag="PLQ70_02330"
     CDS             309..1079
                     /locus_tag="PLQ70_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQE33480.1"
                     /translation="MKKSFLKTLLFCAFYLLFSCNNETSKPTKKPYKAGVILSFDDAY
                     VNEWYATDQKLKKYGWKGTFFVCKINTLRHYQIQKLLKLQKEGHEIAGHTLNHINAVD
                     FLKIHSINKYMNQEIDPMLKLMKFYGLNVSSFAYPYGGRTQALDAALLKKFKIIRGRA
                     FCEKNASKQGCYFNNSNLVFSFSIDDTHNHFNIPHLLKLLEYAKKNHKILILNSHKTA
                     DIITGDYQTKNATLEFICQYIKRNNMNFYTISDLNKFE"
     gene            1247..3487
                     /locus_tag="PLQ70_02335"
     CDS             1247..3487
                     /locus_tag="PLQ70_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQE33481.1"
                     /translation="MTPTEKVTEVAQHLEPLISDLGLILMTAGIAVLIFKKLKQPLVL
                     GYLIAGFLAGNHFDFFPSVKEIKSVEVWAEIGVIFLLFSLGLEFSFKKLMKVGGTASI
                     TAGTQIIAMCVLGYSVGQWMDWSKMDSIFLGVIISISSTTIILKSFDELGVKTQKFAG
                     IVIGSLIVQDILAILMMVLLSTIAVSNQFSGVDLLMSVLKLMFFLVIWFLGGIFIIPT
                     VLKKTKNLLTDEMLLILSIALCLTMVGLAANVGFSPALGAFIMGSIIAETTQAEHIEH
                     LVKPVKDLFGAVFFVSVGMLIAPEVLYEHAIPVIILSFVTIFGQAISSTFGALLSGQP
                     LKDSIRTGMSLSQIGEFSFIIATLGVTLKVTNSFLYPIVVAVSAVTVFTTPFMIKYSL
                     PFSEYLEHKLPRKWTKRIERYSASTQAIKTVSLWQTVINAFLIQVIVLVVIILGIILI
                     SSRFILPLVEDYHIGNALGALITLGMVSPFLWALAFRRVATAEVEQLMLDRKSRGPLT
                     MLFFIRILLSIFFIGLLLNTFFSQRITWIALVFAIVIYLIFQKKLHIQYHKIENHFLA
                     NLNGREIDKAKRSRSDLTPWDGHMAVFDIVTESNIAGETLKNLQIREQLGVNIVSIKR
                     GEITIHIPQANERIFPGDEVCVIGTDKQVQEFKKYLDQHETDVSPEIKEPDIVLRQIE
                     LKNHTFLGKSIKDSKLREKTKGLIVGIEKRGNRTLNPESDVILERDDILWIVGDKKLL
                     ADLAHD"
     gene            complement(3510..4124)
                     /locus_tag="PLQ70_02340"
     CDS             complement(3510..4124)
                     /locus_tag="PLQ70_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQE33482.1"
                     /translation="MNKIAVVVLAAGNAKRLGRSKQLLPWGNSTLLEHIVKNVLTIDA
                     YKFFVVLGAYSNEIIEKTNLSEATLLINSKWQQGLGSSIASAITEIDNKYSELSAVLL
                     VLADQPFISSVHLNAMIKLHLKEKESIIITKKENYKGVPVLFPKKFFGELMSLSNDEG
                     AKQIVNRNKNCVKEIVSQDNIADIDTFEAYETLLKIFNNLVKNG"
     gene            complement(4143..5150)
                     /locus_tag="PLQ70_02345"
     CDS             complement(4143..5150)
                     /locus_tag="PLQ70_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HQE33483.1"
                     /translation="MMHELLKLKESYILAQAMGLKSVMATVVDIEGSSYRRPGVRMLI
                     FENDTMVGAVSGGCVENEILKQSQSVLLDNKAKIMVYDGRYRLGCEGVLYILLEPFFP
                     DAAVWDAFENTLQQRQSLKIESFYTKTAGSHSGLGSVFHFEGNQFTFSNTQLDKSLPN
                     FIQLLKPRFHLIIIGSEHDAVQLCHFASLTGWEVTVVASAKDYKTKANFPGATRLVHL
                     SAEEINTLSIDMDTAVVLMTHSYVNDLKFLVGLKNKQPAYLGLLGPVKRRNLLLNEFF
                     EYTESFNEGLLEVTYGPSGISIGAETPQEIAISIIAEILSVIRKKELMSLKYKNSAIH
                     S"
     gene            complement(5341..5928)
                     /locus_tag="PLQ70_02350"
     CDS             complement(5341..5928)
                     /locus_tag="PLQ70_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33484.1"
                     /translation="MGLTFISAFGLAFSVSLHRPTEKDYISISPLKNDSIASVEAFKQ
                     VYKVLMSPRCVNCHPKGDIPLQGDNSVIHSMQPKRGKDGKGLYAMKCANCHQQSNSPG
                     LNAPPGNPNWHLPSADMKMVFEGKTAHELAKQLLNPELNGHKDIKMLIAHADDGLVKA
                     GWNPGEGRTVPPLTYAQFKKAWITWLQTGAYAPAQ"
     gene            complement(6160..8250)
                     /locus_tag="PLQ70_02355"
     CDS             complement(6160..8250)
                     /locus_tag="PLQ70_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQE33485.1"
                     /translation="MNSRRNFLKVSALTGGGILFGLVFPSFETNDTSTALADYMPNIY
                     IKIKPDGKIVLMAPNPEIGQGVKTSLPIIIAEELGVDWKKIEVELAPLDNRYGRQTAG
                     GSGAIRGRYADLRKMGAAAREMFVNAAAQIWSVSAADCIADDGMVIHKSSGKKLSYGE
                     LASKAVTMQIPANPTLKDPKDFKYIGTRITDVDVHSIVTGKPLFGIDTRREGMLFAMI
                     ARSPAYGKTLGNVDDTKARKVNGVKNVIKLKNSVAVLATSTWAAKKGRDALVIQWNTT
                     GKLENSSDHFAGFKEILEKGPTTVGRNDGDVEIAKQKATKVLDVYYEIPTLSHCQMEP
                     LNFFADVRDGKVELYGPTQVPDQLRNDVAEKLGINKDNITVVVPRQGGGFGRKLMTDN
                     GVEAALVSAAAQSPVQVQWTREDDMQNDFYRPAEMYRYRAALTANELLAWHQSAVGIG
                     RGIRGDSYVAGAMVNYRSDSQGFNSNTPTGWWRAPGACTMAYVAESFFDEICLELKKD
                     PVAFRLDFFKKAKDAPVGRLGYDPIKYKTVVELVAKMSNWGTTPTGVYRGFATWFSFG
                     SYAAQVVEVKLEDGKPRVTKVYCAVYCGRVINLSGAENQIQGAIVDGINHAMFPKVTF
                     VDGAVQETNFNTYNFLRMYDAPLDIEVKFVESDEAPTGLGEPALPPIAAALGNAIFAA
                     TGKRLRRLPFAGQL"
     gene            complement(8262..8726)
                     /locus_tag="PLQ70_02360"
     CDS             complement(8262..8726)
                     /locus_tag="PLQ70_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQE33486.1"
                     /translation="MAKYSLSINGKIMSVEADDDTPLLWVLRDNLNLVGTKFGCGVAQ
                     CGACTVDLDGEALRSCVLPVSAVGKRKVVTIEGLSEDGDHPLQKAWEELDVSQCGYCQ
                     SGQLMAASTLLKSTPDPTDQQIDEAMTNICRCGTQVRIRKAIHLAAKKLNTK"
     gene            complement(8916..10388)
                     /gene="guaB"
                     /locus_tag="PLQ70_02365"
     CDS             complement(8916..10388)
                     /gene="guaB"
                     /locus_tag="PLQ70_02365"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQE33487.1"
                     /translation="MIAHNSKIVGEGLTYDDVLLVPNYSNVLPREVSIKSKFSRNISL
                     NVPIVSAAMDTVTESDMAIAMAQEGGIGVLHKNMTIEQQATKVRKVKRAESGMIIDPV
                     TLPLTSNVADAKAAMKEYGIGGIPIVDENRILKGIVTNRDLRFEKNNARPIIEIMTSE
                     NLVTVAEGTSLQEAEVVLQGYKIEKLPVINAKNELVGLITFRDITKLTQKPNANKDKF
                     GRLRVAAALGVTADAVERATALVNAGVDAVIIDTAHGHTKGVVDVLKAVKAKFPELDV
                     IVGNIATAEAAKYLVENGADGVKVGIGPGSICTTRIVAGVGFPQFSAVLEVAAAIKGT
                     GVPVIADGGIRYTGDIPKAIAAGADCVMLGSLLAGTMESPGETIIFEGRKFKSYRGMG
                     SVEAMQEGSKDRYFQDVEDDVKKLVPEGIVGRVPYKGELNESMQQFIGGLRAGMGYCG
                     SKDIATLQDTGRFVRITSSGITESHPHNVTITKEAPNYSR"
     gene            10555..13056
                     /locus_tag="PLQ70_02370"
     CDS             10555..13056
                     /locus_tag="PLQ70_02370"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HQE33488.1"
                     /translation="MAPSNYSLRGLSDAEVTQSREKNGFNSLDHQVKNHFITSLIEMI
                     KEPMFLLLLVATSIYFIMGDFADGIFMTVAIILVSTISLYQESKSRNAIEELKKLTQP
                     KSKVIRNGEIIEIPSEEIVLGDCIQTEEGTFVPADGFIIQSNDFSVNESILTGESLAV
                     FKNEKAEMNTVFQGTIVASGLAICKVTAIGNQTQLGKIGKSLESIAEEKTPLQLQIGN
                     FVKKMSIIGLVIFGIVWAINFYNSRVLLDSLLKALTLAMSIIPEEIPVAFSTFMALGA
                     WRLMKMGIIVKHTKTVETLGSATVICTDKTGTITENKMSLAQLYLFSTNQLITTKEHY
                     NEAEQELISLSMWASEPIPFDGMEIALHESYSKSTLNDERPHYKLIHEYPLSGKPPMM
                     THIFENKTGNRIIAAKGATEALIACSNLSKDQIQRINEANEAMSKEGFRVLAVGVSLY
                     SGKEFPKTQQEFAFEFKGLVAFYDPPKENIKTVFDTFYKAGIQVKIVTGDNATTTSTI
                     AKQIGFKNPENTLNGDELMTINEASLKEKVMETTIFTRMFPEAKLKIIQALKENNQIV
                     AMTGDGVNDGPALKSAHIGIAMGKKGTEIAKQAANLILIDDDLSKMTDAIAMGRKIYI
                     NLKKAIQYIISIHIPIVLIVFIPLVLGWIYPNIFSPVHIIFLEIIMGPTCSIIYENEP
                     MESNLMLEKPRPLTNTFFNLKEITISIIQGLAITLGLLFVYQHCVQENASESVTRTFV
                     FLTLIASNVFLTLANRSFYYSIFTTIRYKNNLVLVIIGATISITSLLLVVPIFSEFFM
                     FEALSIGQIGLSILVGFVSVMWLEIYKIFKRSIRE"
     gene            complement(13064..14452)
                     /locus_tag="PLQ70_02375"
     CDS             complement(13064..14452)
                     /locus_tag="PLQ70_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HQE33489.1"
                     /translation="MRKISLFIILISMSCFSQNKQLLYNFTSVPQSMLTNPGSDVNYN
                     WFVGIPLLSGFSANVSSSSFSVYDLFANNGVDFNTKLRNVVFSTSRNDKVTLNEQLEV
                     FSGGFKLGDWQESSYFSFGMYQEMDVLTYMPKDYAILALDGNKDYLGKVFDMGDLNAR
                     GELLSVFHLGYHKKVNENLILGVRGKIYSSIFNVSSTKNSGYFYTIQGTNNVYDQVIS
                     ANGLVNTSGLTNYLENYSGNAQSDIMKKTLLGGNLGLGLDLGFTYYPKENLQFTASLI
                     DIGFIKHTKEIESYRYKGYYNYVGLTPDFVLGNSNDILDEFNDAIPLDTLHTKYTTWR
                     PLKFNSSIQYSFGDGRSSDCNCSGGNKIKYANSVGAQLFAMTTPRTPLMALTAYYRRQ
                     IFESLEMKATYTVDSFSAKNIGLGLSTTIGKVNFYILADNLLEYKDISKARSLGFQMG
                     LNIIFPDKNTFD"
     gene            complement(14449..15030)
                     /locus_tag="PLQ70_02380"
     CDS             complement(14449..15030)
                     /locus_tag="PLQ70_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33490.1"
                     /translation="MNIFVENVIYQYITIMNKFLRITFLSVFFLSCSSDLDFEQVNDL
                     KLEPIAVANLAAFDIKANQFVVGGVEQSLVGDVMDFDVFSDVDFSKNLSRVDLFFEFN
                     NTINRAYNVNFYLLDDNNSKLYTIPFFVPANSGSPIVISKTEIFENTKLDILIKTKKV
                     AFVITMLPGTPLTDASVGNLKMRSSATIYFLGQ"
     gene            complement(15043..15906)
                     /locus_tag="PLQ70_02385"
     CDS             complement(15043..15906)
                     /locus_tag="PLQ70_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HQE33491.1"
                     /translation="METIKIIECPRDAMQGIKAFIPTDRKVAYIQSLLRVGFDTIDFG
                     SFVSPKAIPQMQDTAEVLARLDLSQTESKLLAIIANTQGAITASQHQPIQYLGFPFSI
                     SENFQMRNTHKTIAESLVTLEEILEIADKTNKEVVAYLSMGFGNPYGDPWNLEIVSEW
                     TEKLATMGVKILSLSDTVGSSTPEAIQYLFSHLIPKYTTIEFGAHLHTTPDKWFEKMD
                     AAYNSGCRRFDGAIQGFGGCPMARDVLTGNMPTEKLLSYCNSKKANTNLSAMSFESAY
                     NEATKIFKTYH"
     gene            complement(16067..16318)
                     /gene="rpsT"
                     /locus_tag="PLQ70_02390"
     CDS             complement(16067..16318)
                     /gene="rpsT"
                     /locus_tag="PLQ70_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQE33492.1"
                     /translation="MANHKSALKRIRSNEKKRVLNRYQHKTTRNAIKALRLSTDKSDA
                     ASKLSSVISMIDKLAKKNIIHDNKASNLKSKLTKHVAKL"
BASE COUNT         5326 a         3133 c         2683 g         5375 t
ORIGIN      
        1 tcgaagctac cttgaaaggg ttgaacaaat tgcttttgaa cgaatcaaaa tttgccgaag
       61 atttagaaaa aaactgggct gttgttgccg aagcgataca aactattttg cgtcgtgaag
      121 cctatccaaa tccttatgaa gccttgaaag gattgacaag aactaatgaa gcgattacca
      181 aagattcgat tcacgctttc atccaaactt tagaagtttc agcagaaata aaagcagaat
      241 taatgcaaat tacaccaagt aatttcttgg ggatttaata aaaaacacat ttaaagcaca
      301 aaataataat gaagaagtct tttctaaaaa cccttttatt ttgtgctttt tatcttttat
      361 tttcgtgcaa taatgaaacc tctaaaccta caaaaaaacc atataaagct ggggtcattt
      421 tgtcatttga tgatgcttat gttaatgaat ggtatgcaac cgatcaaaaa ttaaaaaagt
      481 atggttggaa aggcactttt tttgtttgta aaataaacac tttaaggcat taccaaatcc
      541 aaaaactgct caaattgcaa aaggaagggc acgaaattgc tggtcataca ctgaatcata
      601 ttaatgctgt tgattttctc aaaatccaca gcataaacaa atatatgaat caggaaattg
      661 acccaatgtt gaaattaatg aaattttatg gactaaatgt atcctctttt gcctatcctt
      721 atggtggacg aactcaagca ttggatgcag ctttactcaa aaaatttaaa atcataagag
      781 gaagagcttt ttgcgaaaaa aacgcatcta aacaaggatg ttatttcaat aactctaatt
      841 tagtttttag ctttagtatt gacgacacac acaatcactt caacatccct catctgctga
      901 aattattgga atatgccaag aaaaaccata aaattttgat tttgaatagt cacaaaacag
      961 cagatataat tacaggtgat tatcaaacta aaaatgctac attagaattc atttgccaat
     1021 atataaaacg taacaatatg aatttttaca ccatatcaga tttaaataaa ttcgaataaa
     1081 agcattttat aaaatttaat tccctttgaa aaaatattaa aaacatattt tcaaagggat
     1141 ttttttattt aaaatagaaa ctaaaatgct tttttaaaaa gattaacttt tgaaaaaagt
     1201 ttttttgtat cttaccaaaa atcaacagag aaataagtac aatcctatga ctccaacaga
     1261 aaaagtaacc gaagttgcac aacatttaga acctctaatc agcgatttag gactgattct
     1321 tatgactgct ggaatagccg ttttgatatt taaaaaatta aaacaaccat tagttttagg
     1381 ctatttgatt gctggttttt tggcaggaaa ccattttgat ttttttccat cggtaaaaga
     1441 aatcaaaagt gtagaagttt gggcggaaat tggagttatc ttcctattgt tcagtttggg
     1501 attagaattt agtttcaaaa aattaatgaa agtgggcgga actgcctcta tcactgcggg
     1561 cacacaaatt attgccatgt gtgttttggg ttattctgtt ggacaatgga tggattggtc
     1621 caaaatggat agtatttttt tgggtgtaat catttccatt tcttctacca caattatttt
     1681 aaaatccttt gatgaattag gcgtaaaaac tcaaaaattt gctggaatcg ttattggttc
     1741 gctaatcgtt caagacattc ttgccatttt gatgatggtt ctattatcaa caattgcggt
     1801 gagtaatcaa ttttctggag ttgacttatt aatgtcggtt ttgaaactta tgttttttct
     1861 tgtcatttgg tttttgggag gtatttttat cattccaact gtcctcaaaa aaaccaaaaa
     1921 tttattgaca gatgaaatgc tacttatcct atcgatagcc ttatgtctaa cgatggttgg
     1981 attggctgct aatgtcggtt tttcaccagc tttaggcgct tttataatgg gttccatcat
     2041 tgccgaaacc actcaagccg aacacattga acatttagta aagcccgtaa aagatttatt
     2101 tggagctgta ttctttgtat ctgttggaat gttaattgca cctgaggttt tgtacgaaca
     2161 cgccattcca gtaattatcc tttcgtttgt tactattttt gggcaagcca ttagttcaac
     2221 ttttggcgct ttattatcag gacaaccctt gaaagattct atcagaacag ggatgagttt
     2281 atcacaaatt ggggagtttt cttttatcat cgccacttta ggagtaacat tgaaagttac
     2341 gaattctttt ttatatccaa ttgtagtggc ggtttcggcc gttacagtat ttacgacgcc
     2401 gtttatgatt aagtactctc ttcctttttc ggaatatttg gagcataaat tacctcgaaa
     2461 atggacgaaa cgaattgaac gttattcggc gagtactcaa gccataaaaa ccgttagttt
     2521 atggcaaacg gtaatcaatg cgttcctaat tcaggttatt gtacttgtag taatcattct
     2581 tggaattatt ctgatttctt ctcgatttat attgcctttg gttgaggatt atcatatagg
     2641 aaatgcactt ggagccttga taacattggg aatggtatct ccgttcttat gggcactagc
     2701 tttccgaaga gttgccaccg cagaagtcga gcaattaatg ctagaccgca aatctcgtgg
     2761 tccactaacg atgctgtttt tcattcggat tttattatct attttcttta ttggactttt
     2821 attaaatacc tttttctccc aaagaattac ttggattgcc ttggtgtttg ccattgtaat
     2881 ttatttgatt tttcaaaaaa agctccacat ccaatatcac aaaattgaga atcactttct
     2941 agccaattta aatggaagag aaatcgataa agccaaaaga agtcgaagcg atttaactcc
     3001 ttgggatggg cacatggctg tatttgatat tgttacggaa tcgaatattg ctggtgaaac
     3061 tttaaaaaat ttacaaattc gagaacaatt gggcgtgaat attgtttcta tcaaaagagg
     3121 cgaaatcacg attcatattc cccaagcaaa cgaacgtatt tttcctggag acgaagtttg
     3181 cgttattgga acagacaaac aggtgcagga atttaaaaaa tatttagacc aacacgaaac
     3241 cgatgtttct cctgaaatca aggaaccaga tattgtttta cgccaaattg aattgaaaaa
     3301 tcacactttc ttgggaaaaa gcattaaaga ttctaaatta agagaaaaaa caaaaggatt
     3361 gattgtgggc atcgaaaaac gaggaaatag aaccttaaac cctgaatcgg atgtgatttt
     3421 ggaaagagac gatatcttat ggattgtagg tgataaaaaa ctattagcag atttggctca
     3481 cgattaagaa taaaacaaac tttatgcaat caaccatttt tgaccagatt attaaaaatt
     3541 ttaagtaaag tttcatacgc ttcaaaagta tctatatcgg ctatattgtc ctgactgaca
     3601 atctctttga cacaattctt attcctattg acaatctgtt ttgcaccttc atcattggat
     3661 aacgacataa gttccccgaa gaattttttt gggaataaaa ctggtacgcc tttgtaattt
     3721 tcttttttag taataataat cgattctttc tcttttaaat gaagtttaat cattgcattc
     3781 aaatgaacac tactgataaa aggttgatct gctaatacca acaatacagc acttagttct
     3841 gaatatttgt tgtctatttc tgtaattgca gatgctatag agcttcccaa tccctgctgc
     3901 catttgctgt taatcagtag ggttgcttct gataaatttg ttttttctat aatttcattg
     3961 ctgtaggctc ccaaaacaac aaagaattta taggcatcaa ttgtcaaaac attttttaca
     4021 atatgttcta ataaagttga attaccccaa ggcaataact gttttgatct tcctaatctt
     4081 ttggcattgc cagctgctaa aacaactaca gcaattttat tcatgatctt tgaatttaag
     4141 ttttaggaat ggatagcact attcttgtat tttaaagaca ttagttcctt ttttcgtata
     4201 actgaaagta tttcggcaat aatactaatg gctatttcct gaggagtttc tgctcctata
     4261 ctaatgcctg aaggaccata agttacttcc aacaatcctt cgttaaaaga ttccgtgtat
     4321 tcaaaaaatt cgtttaacaa tagatttctt ctcttgacag gaccaagtaa tccaaggtaa
     4381 gcaggctgct tattttttaa tccaacaaga aacttcaaat cgtttacata actatgtgtc
     4441 attaaaacta ctgcagtatc catatctatg gaaagagtat ttatttcttc agctgataaa
     4501 tggacaagcc ttgttgcgcc aggaaaatta gctttggttt tgtaatcttt tgcagatgcc
     4561 acaacggtca cttcccaacc cgttaaagag gcaaaatgac ataattgaac tgcatcatgc
     4621 tctgatccaa taataattaa atgaaaacga ggtttgagca attgtataaa attaggcaag
     4681 gatttatcca attgtgtatt tgaaaacgta aactgatttc cttcaaaatg aaacacggaa
     4741 cctaaaccag aatgactccc tgccgtcttt gtatagaaag attcaatttt taatgattgt
     4801 ctctgctgca aagtattttc aaaggcgtcc caaactgctg catcaggaaa aaaaggttcc
     4861 aaaagaatat acaaaacacc ctcacagccc agacgatatc tgccgtcata aaccataatt
     4921 tttgccttat tatccaataa taccgactgg gattgtttaa ggatttcatt ttcaacacag
     4981 cctccgctaa ccgctcccac cattgtatca ttttcaaata ttagcattcg aactcccggc
     5041 cttctatatg atgaaccttc aatgtcaaca acagttgcca taacgctttt taatcccata
     5101 gcttgggcta aaatgtatga ttctttcaat tttaataatt cgtgcatcat atttaatcac
     5161 ttttcaaaat aatttcacct tcaatattta tggattaaat tggctgcttc ttttcaaaaa
     5221 catttgttta aactaaaatg caaagtgctg tacctgctaa tcccgtatgt tatataaaat
     5281 ttcatttcga taattatcat ccgtattact ttttgttatc ggataatcgt taaaacaatt
     5341 ttattgagca ggcgcataag cacctgtttg caaccaagtg atccaggctt ttttaaattg
     5401 agcataagtc aaaggtggaa cagttcttcc ttcgcccgga ttccatccag cttttactaa
     5461 accatcatct gcatgagcaa taagcatttt tatgtcttta tgtccgttaa gttctgggtt
     5521 aagcaattgc ttggccaact cgtgagccgt tttcccttca aacaccattt tcatgtcggc
     5581 ggatggtaaa tgccaatttg gatttcccgg aggtgcattc aatcccggac tatttgattg
     5641 ctgatgacaa tttgcacatt tcattgcata caaacctttt ccatcttttc ctcttttagg
     5701 ctgcatacta tgaataacac tattgtctcc ctgaagcgga atatcgcctt ttggatgaca
     5761 attaacacag cgcggactca taagtacttt ataaacctgt ttgaaagcct ccacagaggc
     5821 tatactgtca ttttttaacg gactaatgct tatgtaatcc ttttctgttg gccgatgtag
     5881 cgaaacagag aatgctaatc caaaggcaga aatgaatgtt agacccacca atataaattt
     5941 agtttttgtc gacatggtcg tgtgtgtgtt ttgagaattt aaattttatt attttcttcc
     6001 attaatggct ataaccacat agtggtatat caattccctg cctgattttg tgctgggtaa
     6061 tcgttgtttt cagataatcc ttcaataatt tgaataaaaa tatgaaaagg ataattaggc
     6121 aataaaatgt gcttttcttt tgagtaaact aatcaattct caaagctgac ctgcaaatgg
     6181 caacctgcga agtcgcttac cagtagcagc aaaaatagca ttacccaagg cagcagctat
     6241 aggtggcaaa gctggctcac ccaagcctgt tggggcttca tcagattcta caaatttcac
     6301 ttcaatgtcc aaaggagcat catacatgcg gagaaagttg taagtattga aattggtttc
     6361 ttgtacagct ccatcgacaa aagtaacttt tggaaacatg gcgtggttga taccatctac
     6421 aatagcaccc tgaatttgat tttcggcacc acttaggttg ataaccctac cgcaataaac
     6481 tgcacaatat acttttgtga ctcttggttt tccatcttcc aattttactt caactacttg
     6541 agcggcatat gaaccaaagg aaaaccatgt ggcaaaccct ctataaaccc ctgtaggtgt
     6601 tgtaccccag ttgctcattt tagccaccag ctctaccaca gttttatatt ttattgggtc
     6661 ataacccaat cgtcctacag gcgcatcctt tgcttttttg aagaaatcca aacgaaatgc
     6721 cacagggtct ttttttaatt ccaggcatat ttcgtcaaaa aaactttcag ctacataagc
     6781 cattgtacaa gcacctggag cacgccacca acccgtagga gtgttgctat taaatccttg
     6841 actatctgaa cgataattta ccatagcgcc tgccacataa ctatcacccc taataccacg
     6901 gccaatccct acggccgact gatgccatgc caaaagttcg tttgccgtaa gtgctgcgcg
     6961 ataacggtac atttcggctg gtcgataaaa atcattttgc atatcatctt cacgagtcca
     7021 ctgaacctga acagggcttt gggctgctgc tgaaaccaaa gcggcttcta caccattatc
     7081 tgtcataagc ttgcgaccaa aaccgccacc ttgccttggc actactactg tgatattatc
     7141 tttgttgata cctaattttt cagctacatc attccttaat tggtctggaa cttgggtcgg
     7201 cccatacaat tctacttttc catctctcac atcagcaaaa aaattgagtg gttccatttg
     7261 gcaatgtgaa agggtaggta tttcataata tacatcaagt acttttgttg ctttttgttt
     7321 tgcaatttct acatctccat catttctgcc tacggtggtt ggtccctttt ctaaaatttc
     7381 tttaaaacca gcaaaatggt ctgatgaatt ctccagtttt ccggtagtgt tccattgaat
     7441 aaccaaagca tcacgtcctt tttttgctgc ccaagtagat gtagctaaaa cagctaccga
     7501 atttttgagt ttaatgacat ttttaacgcc atttactttc cgagccttag tatcatctac
     7561 attgcccaat gtttttccat aagctgggga gcgagctatc atggcaaaca acattccttc
     7621 tctacgggta tctatcccaa agaggggctt gccagtaact atactgtgaa catcaacatc
     7681 ggtaattcgt gtgcctatgt atttaaaatc ttttgggtct tttaaagtgg gattggctgg
     7741 tatttgcatt gtgactgctt ttgaagctaa ttctccatag ctcaattttt ttccagaact
     7801 tttatgaata accattccat catcggcgat acaatctgct gctgacacac tccatatttg
     7861 ggcagcagca tttacaaaca tttcacgagc agcagcaccc attttacgta aatctgcata
     7921 tcgccctcta atagcgccgc taccacctgc tgtctgcctt ccatacctat tgtccaaagg
     7981 cgccaactct acttctattt tcttccaatc tacacccaat tcttcggcaa ttataattgg
     8041 aagtgaagtt tttactcctt gtccgatttc tgggttagga gccatcagca caatcttgcc
     8101 gtcaggtttt atcttgatat aaatattggg catataatca gctaaagcgg tagaagtatc
     8161 atttgtctca aagctgggaa ataccaaacc gaaaagtatt ccaccacctg ttagggcgga
     8221 aacttttaaa aaatttctac gagaattcat aaaatccata actatttagt atttaatttt
     8281 ttagctgcta aatgaatagc tttgcgaatc cttacttgtg taccacaacg gcagatattt
     8341 gtcattgcct cgtctatttg ctgatcagta gggtctggag tgcttttaag caaagtagaa
     8401 gcagccatta attgtcccga ttgacaatag ccacattgcg aaacatctag ttcttcccaa
     8461 gccttttgaa gtgggtgatc gccatcttca ctaagtcctt ctatggtaac aacttttctt
     8521 ttacctactg cagaaacggg taaaacacaa gaacgtaaag cctcaccatc gagatctacg
     8581 gtacatgcgc cacattgtgc cacaccacat ccgaatttag tgcctaccaa atttagatta
     8641 tctcggagaa cccataataa aggggtatcg tcatcagctt ccaccgacat tattttaccg
     8701 ttgatagaaa gagagtattt tgccatattg tactatttta aaaatttatt ttttattgat
     8761 aataaaaaaa acattgctaa aattaaccaa ttattatcat tttacctcat tattcgcaaa
     8821 ttaataaatt tgcagaaata acattacatt acaataatca aaaaaggtgc aagaactaaa
     8881 tcttgcacct tttttatttt aaggatatac tatttttatc tcgaataatt cggagcttct
     8941 ttggtaatcg tcacattatg tggatgactt tcggtaattc cgctagaagt aatacgaaca
     9001 aagcgtccag tatcttgtaa agtagcaata tctttagagc cgcaatatcc cattccagca
     9061 cgaagaccgc caatgaattg ttgcatactt tcgttcaatt cacctttata aggcactcgg
     9121 cctacgattc cttctggaac taatttctta acatcatcct caacgtcctg aaaataacgg
     9181 tctttagatc cttcttgcat cgcctcaaca gaacccattc ctcggtaaga cttgaatttt
     9241 cttccttcga aaatgatggt ttctcctgga gattccattg ttcctgctaa aagtgaaccc
     9301 aacattacgc aatcagcacc ggcagcaatc gctttaggaa tatctccagt gtaacgaatt
     9361 ccaccatcag caataactgg aactcctgtc ccttttatag cagcagctac ttcaagaaca
     9421 gcagaaaatt gtggaaaacc aacaccagca acgattcttg tagtacaaat agaaccaggt
     9481 ccaattccta ctttcacacc atcggcacca ttttctacta aatatttagc agcttcggca
     9541 gtagcgatat ttccaacaat tacatctaat tctgggaatt tggctttcac ggccttcaat
     9601 acatctacca caccttttgt atgtccgtga gcagtatcga taattacggc atccactcct
     9661 gcgtttacta aagctgtagc tctttcgaca gcatcggcag taacacccaa agcggctgct
     9721 actctcaaac gaccaaattt atctttattg gcgtttggtt tttgagttaa tttagtaata
     9781 tctctaaacg tgattaaacc aaccaactca tttttagcat ttattacagg aagtttttca
     9841 attttatacc cttgcaaaac tacttcagct tcttgaagtg aagttccttc ggcaacagtt
     9901 accaagtttt cactggtcat tatttcgatg ataggtcttg cgttattttt ttcgaaacgc
     9961 aaatctctat tggttacaat tccttttagg attctattct catcaacaat tggaatacca
    10021 ccaataccat attctttcat agctgcttta gcatcagcaa cattagatgt caaaggcaaa
    10081 gtaacaggat cgataatcat tcccgattcg gcacgtttta ctttacgaac tttagtagct
    10141 tgttgttcga tagtcatatt cttgtgcaaa actccaattc ctccttcttg tgccatagca
    10201 attgccatat cactttccgt aacggtatcc atagctgcag atacaattgg aacattaagc
    10261 gaaatatttc tagaaaattt tgatttaata cttacttcgc gaggaagtac attagagtaa
    10321 tttggaacca gcaatacatc atcgtaagtt aaaccttcgc cgacaatttt tgaattatgt
    10381 gctatcattg caatttgaag ttgtagttaa attgcgtgca aatatagaca atcttaagga
    10441 aacaaacatc attcatttgc tataaatgtt gtaaattgca gtacaatact atttttaagc
    10501 ctattttgaa tattttcttt tataaatctt aaaaagattt ttgaaaaaca caccatggca
    10561 ccatcaaact attcattaag aggactttct gacgcagaag ttactcaatc ccgagaaaaa
    10621 aacggtttca attcattaga ccatcaagtt aaaaaccatt ttatcacttc tctaatcgaa
    10681 atgattaaag aacctatgtt tttgctgctt ttggtcgcca cgagtattta ttttataatg
    10741 ggtgattttg ccgatggaat ttttatgacc gttgctatta ttttggtttc aaccatttca
    10801 ctgtatcaag aatcgaaaag cagaaatgcc attgaagaac taaaaaaatt aacccaaccc
    10861 aaaagcaaag tcatcagaaa cggagaaatc atcgaaattc caagtgaaga aattgtcttg
    10921 ggcgattgca ttcaaaccga agaaggcacc tttgttcctg ccgatggttt catcattcag
    10981 tcgaatgatt tttcggtaaa tgaatctatc cttacaggtg aatctttggc tgtttttaaa
    11041 aatgaaaaag ccgaaatgaa taccgttttt caaggaacga ttgtagccag tggtttggca
    11101 atttgcaaag ttactgccat tggcaatcaa actcaactgg gtaaaattgg caaaagcctt
    11161 gaatctatcg ctgaagaaaa aactccttta cagttacaaa tcgggaattt tgtaaagaaa
    11221 atgtcaatta ttggcttagt gattttcgga attgtttggg caataaattt ctataattct
    11281 agagttcttt tagacagttt actaaaagct cttactctag cgatgagcat aattcccgag
    11341 gaaattccgg ttgcattttc gacttttatg gctttgggag cttggagatt aatgaaaatg
    11401 gggattattg taaaacacac aaaaaccgtc gaaacccttg gaagtgccac agtaatttgc
    11461 accgataaaa caggaactat aactgaaaac aaaatgagtt tggctcaatt atacctcttt
    11521 tcaaccaatc aattaataac aacaaaagaa cactataacg aagccgaaca agaactaata
    11581 agtctgtcga tgtgggctag cgaaccaatt ccgtttgatg gaatggaaat agctcttcac
    11641 gaaagttatt caaaatcgac actaaatgac gaaagacctc attataaatt aatacacgaa
    11701 tatcctttga gtggcaagcc gccaatgatg actcatattt ttgaaaataa aacgggaaat
    11761 agaattattg cagccaaagg agctacagaa gcattgatag cctgctctaa tctttcgaaa
    11821 gaccaaatac aacgcatcaa tgaagcaaat gaagcgatgt cgaaggaagg ttttagagtg
    11881 ttagcagttg gtgtttctct ctattcaggc aaagaatttc caaaaacaca acaggaattt
    11941 gcattcgaat ttaaaggatt ggttgctttt tacgatcctc caaaagaaaa cataaaaaca
    12001 gttttcgata ctttttataa agctggaatt caagtaaaaa ttgtcaccgg tgacaatgct
    12061 acaactactt caactattgc caaacaaatt ggatttaaaa atcccgaaaa cacactaaat
    12121 ggcgatgaat taatgacaat taacgaagct tctttgaagg aaaaagttat ggaaactacg
    12181 atttttacca ggatgtttcc cgaagccaaa ctaaaaatca ttcaagcatt aaaagaaaat
    12241 aatcaaattg tagccatgac cggcgatggt gtaaacgacg gtccagcctt aaaatcagct
    12301 catattggga ttgctatggg taaaaaaggt actgaaattg ccaaacaagc cgccaatctc
    12361 atcttgattg atgatgattt gtcaaaaatg actgacgcta ttgcaatggg tcgaaaaatc
    12421 tacattaacc ttaaaaaagc catacaatac atcatttcta ttcacattcc tattgtactg
    12481 attgtgttca tcccattggt tttaggatgg atttatccca atattttctc gccagtacac
    12541 attatttttc tggaaataat tatggggcca acttgttcta taatttatga aaatgaacca
    12601 atggaatcta atttgatgct tgaaaaacca cgtccgctaa ccaatacatt ttttaatcta
    12661 aaggaaataa cgattagtat tattcaagga cttgcaatca ctttgggact tttatttgtc
    12721 tatcaacact gtgttcaaga aaatgcttca gaaagcgtga ctagaacttt tgtttttctg
    12781 actttaattg cttcgaatgt attcttaaca ttagccaatc gctcctttta ttattcgata
    12841 tttacaacca ttcgatacaa aaacaacttg gttttagtaa tcattggagc tacaattagt
    12901 attacaagtt tattgttggt agtcccaatt ttttctgaat tctttatgtt tgaagcgcta
    12961 tcaattgggc aaataggctt gagtattttg gttggatttg tatcggtaat gtggttagaa
    13021 atctataaaa tattcaagcg tagcattcga gaataatcaa gatttaatcg aatgtatttt
    13081 tatcaggaaa aataatattc aatcccattt gaaatcccaa actacgggct ttgctaatgt
    13141 ctttgtattc taaaagattg tcggcaagga tataaaaatt gactttcccg atggttgttg
    13201 acaatcctaa gccaatattt ttggctgaaa atgaatcaac cgtataagta gctttcattt
    13261 ctaatgactc gaaaatttgt cttctataat aagcagtcaa agccataaga ggcgttctag
    13321 gcgttgtcat agcaaacaat tgggctccaa cagaattggc atacttaatt ttatttccac
    13381 ctgaacaatt gcaatccgaa cttctaccat ctccaaaaga atattgaatc gaagaattga
    13441 atttcagcgg tcgccaagta gtatacttag tatgcaaagt atctaatgga atggcgtcat
    13501 taaattcatc taagatatca tttgagttgc caagcacaaa atctggagtt aaacctacgt
    13561 aattataata tcctttatag cgataacttt cgatttcttt agtgtgtttt atgaaaccaa
    13621 tatctattaa acttgccgta aattgaagat tttctttggg ataataagta aatcctaaat
    13681 ctaaacccaa acctagattt cctcccagca gcgttttttt cataatatca ctttgagcat
    13741 ttcctgaata attttcgaga taattggtca aacctgaagt gtttactaat ccattagctg
    13801 agataacctg gtcgtaaaca ttatttgttc cttgaatggt ataaaaatag cctgaatttt
    13861 tagtagaaga tacattaaaa atactggaat atatctttcc gcgaacgcct aaaattaaat
    13921 tctcgtttac ttttttatga taacccaaat gaaaaaccga aagtaattcg cctcttgcat
    13981 tcaaatcgcc catgtcaaaa actttaccca aataatcttt gtttccgtct aaggctagaa
    14041 tagcataatc tttgggcata taagtcaaaa cgtccatttc ttgatacatt ccgaaggaga
    14101 aataagagct ctcctgccaa tcacccaatt taaaaccgcc actaaaaact tctagttgct
    14161 cattcaaagt aaccttatca ttccttgaag ttgaaaacac aacatttctt aacttggtat
    14221 taaaatcaac tccattatta gcaaacaaat cataaactga aaaactgctt gaactcacat
    14281 ttgcagaaaa tccagacaac aaaggaatac caacaaacca attgtaattc acatccgaac
    14341 ccggatttgt caacatagat tgaggcacag aagtgaaatt atataaaagt tgcttgtttt
    14401 gcgaaaaaca actcatagaa attaatataa taaataagct gatttttctc attgccccaa
    14461 aaagtaaatt gtagcacttg aacgcatttt caaattcccc acactagcat cagtaagcgg
    14521 cgttccaggt aacatcgtaa taacaaatgc cactttttta gtctttatta atatgtctag
    14581 tttggtattc tcaaatattt ctgtctttga tattacaatt ggcgaaccac tattagcagg
    14641 tacaaaaaac gggatagtat aaagcttact attattatca tcaagcaaat aaaagttaac
    14701 attataagct ctatttatag tgttattaaa ttcaaaaaac agatcaactc tacttaaatt
    14761 tttagaaaaa tcaacatcgc taaaaacatc aaaatccatc acatctccaa ccagagattg
    14821 ctctactcca ccaacaacaa attgattagc cttaatatca aaagcagcca aatttgctac
    14881 tgcaatgggt tctaatttca aatcattaac ttgctcaaaa tctaaatcgc ttgaacagga
    14941 taagaaaaaa acagataaga aagttattct taaaaattta ttcataatag taatgtattg
    15001 gtaaattaca ttttcaacga aaatattcat tttttattat tttcaatgat aggtcttgaa
    15061 tatttttgta gcttcattat aagcactttc aaagctcata gcgcttaaat tggtgttggc
    15121 ttttttggaa ttacaataag acaacaattt ctcagtaggc atattacccg ttaaaacatc
    15181 tcttgccatc gggcaacctc caaaaccttg gatagcaccg tcaaaacgac ggcaacccga
    15241 attataagca gcatccattt tttcaaacca tttgtcgggc gtggtgtgca aatgagctcc
    15301 aaattcaata gtcgtatatt tcgggattaa atgcgaaaat aaatattgaa tggcttcagg
    15361 agtcgagctg ccaacagtat cggaaagcga aagaattttt acccccatag tagctaactt
    15421 ttcagtccat tcacttacga tttccaaatt ccatggatcg ccataaggat taccaaatcc
    15481 catcgaaaga taggcaacaa cttctttatt ggtcttatcc gcaatctcta aaatttcttc
    15541 taaggttact aatgattcag caatggtttt gtgtgtattc cgcatttgaa aattctctga
    15601 aatcgagaat ggaaacccca aatattgaat tggttggtgc tgagaagcgg taattgctcc
    15661 ttgagtatta gcaatgatgg ccaataattt actttctgtt tgggataaat ctaatcgtgc
    15721 caaaacctcg gcagtatctt gcatttgcgg aattgctttg ggcgaaacaa aacttccaaa
    15781 atctatggta tcaaaaccca ctcggagcaa cgattgaatg taagccactt ttcgatccgt
    15841 aggaataaat gcttttatgc cttgcatagc atctcttgga cattcgatga tttttattgt
    15901 ttccatagaa atccaaagat aaggcaaaat cagatttcag gaaatcgttt ttcattaatt
    15961 ttttgaatgc tacaataatc tcaattttca gaatagaagc attatatttt cataaaaaaa
    16021 gccccgaact aatcggggct ttctatatat taactaattg caaatattac aatttagcta
    16081 catgtttcgt taattttgat ttcaaattag aagctttgtt atcgtgaatg atattctttt
    16141 tagctaattt atcgatcata gaaataacac tagacaattt agatgcagca tctgatttgt
    16201 cagtagataa tcttaatgct ttgattgcat tacgagtagt cttgtgctga tatctgttta
    16261 atactctttt tttctcgttg cttctaattc tttttaaagc tgacttgtga tttgccattt
    16321 tttcttaatt tttaattgtt ataaactatc agttaaaaaa gaaaaacctc ccacaattga
    16381 tttctcaatc acttcagttt taactaataa aattaaaata agagacactt atctttattt
    16441 tataaaactt atttacaact aattgtagtc cgtgggggaa tcgaaccccc cttaccagga
    16501 tgaaaacctg gcgtcct
//
LOCUS       DAOSNN010000024        16281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3601_length_16281_cov_4.584063, whole genome shotgun sequence.
ACCESSION   DAOSNN010000024 DAOSNN010000000
VERSION     DAOSNN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16281
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3601_length_16281_cov_4.584063"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PLQ70_02395"
     CDS             <1..1326
                     /locus_tag="PLQ70_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQE33493.1"
                     /translation="TNNEGTPPVLPKGHLHLCSKCGGSGLISSANFPIPDTENYPHVA
                     IIGGGIGGVALAVACLHRRIPFTLFERDTNFEARSQGYGLTLQQASKAIEGFGIVALD
                     EGVISTRHLVHATDGKVIGEWGTRKWIQSEVKTSSKRSNIHIARQSLRLALLEQLGGN
                     DAVKWGHQLIDFKKSDSEGVELHFQVDGNIKTAKSDLVVGADGIRSLVRKLIIGEDST
                     PLRYLGCIVILGICPLSALEDHDNPLLDSATVFQTANGNERIYIMPYTSDSVMWQLSF
                     PMSEKDAKALSTKGPQALKEEACRRTQWHDPIPQILSTTLEAQVSGYPVYDRELLKPE
                     LLEQSEQVTLIGDAAHPMSPFKGQGANQALLDALALARGITRGCRPSSQWRKIGIRKS
                     VLSEFESEMLARSAVKVKDSAEAADFLHSEIVLYESDAPRRRFIKKKEA"
     gene            complement(1366..2157)
                     /locus_tag="PLQ70_02400"
     CDS             complement(1366..2157)
                     /locus_tag="PLQ70_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQE33494.1"
                     /translation="MESLIIKYRSLGIADVLHYEKFNLISISHHSTKIEGSTLTALET
                     QILLDDGLTPKGKSVSDTLMVTDHFKALHFIIDKARNKTAVTTELIKDINSYVMKSTG
                     GIYNTILGEIDASKGMFRKRNVTAGSSYFPNYDKVERLTNELVSFIKTKMNDNLSIDD
                     QLNLSFDVHFNLVSIHPFYDGNGRTSRLLMNYIQAFYGIPLAIVDSEDKQDYIQALID
                     TREKEDIAIFRNFMKNQYEKYLLQEIKKFEEGLSSKSNKGFSFLF"
     gene            complement(2314..2387)
                     /locus_tag="PLQ70_02405"
     tRNA            complement(2314..2387)
                     /locus_tag="PLQ70_02405"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2351..2353),aa:Arg,seq:tct)
     gene            complement(2416..2493)
                     /locus_tag="PLQ70_02410"
     tRNA            complement(2416..2493)
                     /locus_tag="PLQ70_02410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2456..2458),aa:Pro,seq:tgg)
     gene            complement(2508..2594)
                     /locus_tag="PLQ70_02415"
     tRNA            complement(2508..2594)
                     /locus_tag="PLQ70_02415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2558..2560),aa:Ser,seq:gct)
     gene            2900..4078
                     /locus_tag="PLQ70_02420"
     CDS             2900..4078
                     /locus_tag="PLQ70_02420"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HQE33495.1"
                     /translation="MNKKVVIVAAVRTPIGSFMGGLSTVSAPKLGAIAIKGALDRIQL
                     DASLVDEVFMGNVIQAGVGQAPARQAAIFAGLPNSVVCTTVNKVCASGMKSVMLAAQA
                     ILCGDAEIVVAGGMENMSLIPHYLHLRNGYKFGPASMIDGMQKDGLTDAYDNNAMGVC
                     ADLCADEYKITREEQDAFAIQSYERSAKAWEDGKFENEIIPVAVAQRKGEPIIVSKDE
                     EFTDVKLDKIPSLNAVFTKDGTVTAANASTINDGAAAMILMSEEKAISFGLKPLAYIK
                     SYADAAQEPKWFTTTPAIAINKALKKVDLPIDAVDYFEFNEAFAVVGLANAKILGLED
                     SKVNVNGGAVSLGHPLGCSGARIIVTLLNVLEQNNGKIGAAAICNGGGGASAIVIERI
                     "
     gene            4098..4859
                     /locus_tag="PLQ70_02425"
     CDS             4098..4859
                     /locus_tag="PLQ70_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HQE33496.1"
                     /translation="MFGICNLAIIPLRIEPSDRSEIVSQVLFGEHFEILEQFKQWSKI
                     KLQFDNYEGWVDSKQYQIISESEFHQLSNDVIVLNADLLEYITGPNNLLLPIPLGASL
                     SFLTHSDINTANFNFEGTKISGLKSKKSLLNTAFMYLNAPYLWGGKTPFGVDCSGFTQ
                     MVYKLNGYKLLRDASQQALQGEALSFIEESEPGDLAFFDNEEGNIIHVGIIMQDNYII
                     HASGKVRIDRLDHLGIYNAETNRHTHKLRVIKKII"
     gene            complement(4896..6917)
                     /locus_tag="PLQ70_02430"
     CDS             complement(4896..6917)
                     /locus_tag="PLQ70_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQE33497.1"
                     /translation="MKKVFRFILLSICVFFFASFHKADKETVRKTIALNGNWNFSVDS
                     LSIGESNHWEINGLPKNTSRIVSVPHTWNVEKGLEKYAGNGWYEREFEVTESDLQKTV
                     RVQFDAVYHDAVVYINGKKAGEHIGSGYNRFFITISPFVKKGINKLTVRVNNAFSSNN
                     IPFLKSYDWANDGGIYRSVSLVITEPQAVKNIKVIGTPNGLKGKADIDISFIDNSKID
                     FSKLKLMVEITEENQNTNQVIFNSELKGTVEKSAFKTSLHFDKINPWHFDAPNLYKLK
                     IQLFAEGKLKDEYTTVFGFRSIKVEKNRYVLNGEPIRLMGVEWMPGSNMERGMAETTA
                     DFERNLKLMKNANCIFTRFHWQQDEAIFDWCDRNGILVQEEIPYWGGATMINDSLLAL
                     GKKHLEEMIENHYNHPSIISWGIGNELESHNPENVNRLKTLYNEAKTIDASRLVNYVS
                     NKLQKGKPIQKDYVPDASGEFDMMMFNEYYSTWFGKSLDVIGGELDRISAEYPNKAMT
                     ISEWGLCEPAHKGGDLRRIKEMTEQLKIYGSKDYVAGAIYFCLNDYRTHMGEDFTYAY
                     PQRVHGVCDIFLNPKPSYYTLKKESSPVIIKSVGVKNGTATITLLGKTAIPSYNLKNY
                     SIVSGNEKVTIDKLEPGQEKTFKIKINENHFSIIRPTGFEVADYQFQEK"
     gene            complement(6930..9722)
                     /locus_tag="PLQ70_02435"
     CDS             complement(6930..9722)
                     /locus_tag="PLQ70_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQE33498.1"
                     /translation="MKNILLLIVVSFSICSWSQSLEKKVIDISGQWAVQLDSTDIGLK
                     NGWQNMGFNQMENLPGTTDDFGMGVPNKLKPALSKPQLSHLTRKNSYIGAAWYTREVL
                     IPNDWKGKELLLKLERVIWKTNVWIDGKEVAKGQNSLVSPHYFDLTHYLIPGKKQRIT
                     VRVDNRKQFDTSLDNMAHSYTNETQIIWNGIIGEMNIEAIDAVHIANLQVQPNIDKSK
                     IKVSVSFNNDGKKSQKGVLTVVAIDKESKKSIAAFTQKVQLEKGQSVVDFDYEMGKDV
                     QFWSEFTPELYELKVQLAAGKYNSEKSVDFGMRKFSRNETVLTNNNKAVFLRGTLECN
                     IFPLTGHPPMDKKGWEKVFKTAKEWGLNHLRFHSWCPPKAAFEVADEMGFYLQVELPV
                     WVLTIGKDKNVTDFLYAEAQRMMDEYGNHPSFCLWSMGNELQGDMALLTEMVKNIKAK
                     DNRHLYTSTSFTFEKGHGALPEAEDDFFITQWTNKGWVRGQGVFNSESPTFDKDYKAV
                     LKGFTVPLITHEIGQYSVYPKIDEIAKYTGVLEPLNFISIKEDLERKSLINKAADFTK
                     SSGKLAVILYKEEIERAMKTAGISGFQLLDLHDFPGQGTALVGLLDAFWDSKGLITAA
                     EFRQFSAPVVPLLRFPKATYTNNESFVASLDVSNFSGDALNDKVIEWSIVDNNILIAS
                     GSVTKTINSGYNHELFKVAESLQKITKATKLTFKVSIKDTDYKNQWNIWVYPEQQKID
                     YKDVVYTRSLEEAYKSLQQGRKVLLNPDWKKLSGIEGKFVPVFWSPVHFPKQAGTMGV
                     LCNPKHKVFADFPTDMNTDWQWWDLNVNSTTLIVDSIKGGSPLVEMVDNFANNRKLAS
                     LIEGSIGSGKLVIASFDLSKDLDKRPVAKQMLVSILDYMNGPSFKPYTIENPEVLKLI
                     LKEEKEKAKESAKSIY"
     gene            complement(9766..13137)
                     /locus_tag="PLQ70_02440"
     CDS             complement(9766..13137)
                     /locus_tag="PLQ70_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HQE33499.1"
                     /translation="MEKLISVQSDIITEIADEPITAYLIQSTVNEDGVVRAWEEDIML
                     PTYEIGEEEKNPIFLEKRVYQGSSGSVYPYPVVEKISDVKKDKLYHALFVENKYIKIM
                     ILPELGGRIHMAYDKVKQRHFVYYNQVIKPALVGLTGPWISGGIEFNWPQHHRPSTFL
                     PTDFSIEENADGSKTVWCNEVERMFRTKGMQGFTLHPDKAYIEIKVKIYNRTAFPQTF
                     LWWANPAVVVNDEYKSVFPPDVNFVFDHGKRDVSNFPIATGEYYKQDYSAGVDISKYK
                     NIPVPTSYMAVHSKYNFVGGYEDGVQAGLLHVANHHVSPGKKQWTWGNGDFGLAWDRN
                     LTDKDGPYIELMTGVFTDNQPDFSWLQAYEEKSWVQYFMPYAEVGYVKNATKDAIINI
                     EIDGDRADLILFTTSLFENLKLELKDIDGTILHQEIVSISPYNPYKKNVVIGNIASEN
                     LIFTIYTEDGKVLVEYQEEKPEIKPAPDPAKAALDPKEIASIEQLFLTGLHLEQYRHA
                     TYNPLDYYEEALSRDPKDVRCNNAVGLLMLRRGQFKKSEAYFRTAVEVLTQRSPNPYD
                     GEPHYNLGFCLKIQGKLDEAYTALFKSTWNAAWQDAAFFALAQIDCIRGEWKEALECI
                     DSCLIRNWHNHKARQLKTSILRKLGRTDESLALANESIKMDRFNMGCRFEKYLLTNDE
                     AELNEMNAIMRKWSHGYIEYALDFAGAGLYEEAIQFLKECTQDVTAVYPMVFYTIGYF
                     ESRKGNKNEAIEWYKKASTLSPEKCFPNRIEEVNILQDAILLNTNDAKAPFYLGNFWY
                     AFRQYDNAIECWESSAKIDNQFPTVLRNLALAYYNKLENKVRSQEVLEQAFALDTTDS
                     RIFMELDQLYKKTGKSPEERLAFLEKYSSLVEERDDLCIERIGLYCQIGKYETAKNLI
                     ASRKFHPWEGGEGKVTGLYTLCRVELAKIAITEKRFVDAIQLLKETEVYPHNLGEGKL
                     KNAEENEVYYYKGMAYKGLGDEQNAKANFMEATLGSPEPVQAFFYNDQQPDKIFYQGL
                     AWRELGDEDKARSRFNKLVAHGEKYLFEKSRIDYFAVSLPDLAIWEDDLNIRNQVHCN
                     YVMALGHSGLGNEELSESFYQKVAALDINKYTFRK"
     gene            complement(13159..14535)
                     /locus_tag="PLQ70_02445"
     CDS             complement(13159..14535)
                     /locus_tag="PLQ70_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HQE33500.1"
                     /translation="MNKSNSSYLLFIAIVSALGGLLFGYDWVVIGGAKPFYEVYFHIT
                     DSPAMQGWVMGSAILGCLLGVMASGSMSDKYGRKPLMVLAAILFTLAAVGTGLANSIN
                     MFIVWRIIGGVGIGIASNLSPMYIAEIAPSESRGKFVSINQLTIVVGILAAQIVNMLL
                     ADPIVDGGIAMDSWNVQWGWRYMFWAGGIPAIVFFALILIIPESPRWLASQYKYEKAQ
                     SIFTKIGGLQFATEQIEELKTTVSIVKEAASYKMLFEGKMPKILLLGIVIAAFQQWCG
                     INVVFNYAQEIFSAAGYGVSDILFNIVITGLTNLIFTYVGMVLVDKLGRRALMLFGSI
                     GLACIYALLGICYYFAVTGSAVLILVILAIACYAMTLAPVTWVVLSEIFPVKIRAMAM
                     AVSTFSLWTACFVLTYTFPLLNTGLGAYGTFWLYGIICFLGYLFLRIYLPETKGKSLE
                     QIESELTK"
     gene            14876..15055
                     /locus_tag="PLQ70_02450"
     CDS             14876..15055
                     /locus_tag="PLQ70_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33501.1"
                     /translation="MEHIADGFKGERAIVLPYSIRNFQTNNELTKSLYVTHIGYYPNA
                     KNHFRKRSKELLKTF"
     gene            15052..15753
                     /locus_tag="PLQ70_02455"
     CDS             15052..15753
                     /locus_tag="PLQ70_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQE33502.1"
                     /translation="MIYCEKGKGTITLENEKFNLTENQIFIIPANKTHSYESDPLKPW
                     SIYWIHFKGTKALYYAPNKGKIITLNDSGISNRFELFEEIYQNLEMGFYADNLEYSSI
                     CLKHFLSSIKYIDQFNRIKSLNTSDLDTIPKSIIFMRDNLTNKISLSQIAQYVGYSDS
                     YFRSLFLEKTSFTPIEYLNKLKIQKSCSLLQFSTMKINEIAYHLGYFDPFHFSKSFKK
                     EMEISPREYRKKHKN"
     gene            complement(15818..>16281)
                     /locus_tag="PLQ70_02460"
     CDS             complement(15818..>16281)
                     /locus_tag="PLQ70_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE33503.1"
                     /translation="TKDYETYKKLVAEALEKNPNDADLVFNLGVLSANAKNIADAEKY
                     YKRVMEINPKYANAYINYAALKLEDEKVIIDEMNKLGTSAKDMKRYDVLKVKREDLFK
                     GIIPYLQKAVELEPTNVDVSKTLLNVYSALEMMPEYKALKVKVKELEANKK"
BASE COUNT         5226 a         3214 c         2519 g         5322 t
ORIGIN      
        1 acaaacaacg aaggaacacc tcctgtttta cctaaaggcc atttgcattt atgttcaaaa
       61 tgtggtggtt ctggattgat ttcttccgct aatttcccta tacctgacac agagaattat
      121 cctcacgttg ctattattgg aggcggtatc ggaggcgtgg ctcttgcggt tgcctgctta
      181 caccgcagaa ttcctttcac tctttttgaa cgtgacacta acttcgaggc tcgatctcaa
      241 ggctacggac tcactttgca acaagccagt aaagcaatcg aaggattcgg tattgttgca
      301 ttagacgaag gagtgatttc aacaagacat ttggtgcatg ccactgatgg aaaagtaatc
      361 ggtgaatggg gaacccgaaa atggattcaa tcggaggtaa aaacttcttc gaaacgttcc
      421 aacatacata tcgcacggca atccttacga ttggcactat tggagcaact cggcggaaat
      481 gatgcagtaa aatggggaca ccagttaatt gattttaaga aaagtgatag cgaaggtgtt
      541 gaactccact ttcaagttga tggcaatatc aaaacggcca agtcagatct tgtcgtagga
      601 gccgatggca ttcgcagctt ggtacgaaaa ctaataattg gtgaagacag taccccttta
      661 cggtacttgg gttgtatcgt tattttaggc atttgtcctt tgagtgcgct cgaagaccac
      721 gacaatcctt tactggattc ggccaccgta tttcaaactg ccaatggtaa tgagcgaatc
      781 tacattatgc cttacacctc cgattcggtt atgtggcaac ttagttttcc aatgtctgaa
      841 aaagatgcca aggcactaag caccaaagga ccgcaagcac tcaaggaaga agcctgccgc
      901 agaacgcagt ggcacgatcc cattccccaa attctatcga caaccttgga agcccaagtt
      961 tcaggctatc ctgtgtatga tcgtgaatta ctcaaaccag aattgttgga gcaatccgaa
     1021 caagtcaccc tgattggaga tgccgcacat ccaatgagtc cattcaaagg acagggagcc
     1081 aatcaagcct tgctagatgc actcgcactc gcacgaggaa tcacgagagg atgcaggcct
     1141 tcatcacaat ggcgaaaaat cggaatcaga aaaagtgtgc tatccgagtt tgaatccgaa
     1201 atgttggcgc gtagtgctgt caaagtgaaa gattcagccg aagccgcaga cttcctgcac
     1261 tccgaaattg tgctttatga aagtgatgca cctagaagaa ggtttataaa gaaaaaagag
     1321 gcttgaaaag ttattaagtc aacacaaaaa catccttaaa attgattaaa atagaaaact
     1381 aaatccttta ttggatttag acgaaagacc ttcttcaaat ttttttattt cctgtaaaag
     1441 atatttttca tattgatttt tcataaaatt tctaaaaata gctatatctt ctttctctct
     1501 tgtatcaatc aaagcttgaa tataatcctg cttatcttca ctgtcaacaa ttgccaatgg
     1561 aattccataa aaagcctgaa tatagttcat caacaaacga gaagttcttc cgttaccgtc
     1621 ataaaatgga tgaatactga caaggttaaa atgaacatca aatgataaat tcaattgatc
     1681 atcaatcgac aaattatcat tcatctttgt tttgataaat gacaccaatt cattggttaa
     1741 tctttccact ttatcataat tcggaaaata agaactccca gctgttacgt tccttttacg
     1801 aaacattcct ttgcttgcat cgatttctcc aagaatagta ttgtagatac ctcccgtact
     1861 tttcatcaca taagaattga tatcttttat taactctgtt gtaacggctg ttttatttct
     1921 tgccttatct ataataaaat gcaatgcttt aaaatgatct gtaaccatta aggtatcgct
     1981 gacagatttc ccttttggag ttaatccgtc atcaagtaga atttgagttt ctaatgccgt
     2041 taaagttgaa ccttcgattt tggtagaatg atgacttatc gaaattaaat taaacttttc
     2101 ataatgcaat acatcagcaa ttcctaaact tctatatttt attattagag attccattct
     2161 tatgattttt gaaatattag cataaaaata caactctaaa atcaataaaa tgaataagtt
     2221 aatgaaattt taaacagaag aacatcaatg aaattgactt attaggttgt ataaaacaaa
     2281 aaaacccttg aatatcaagg gttttaaagg tagtgaacgc agaaggattc gaaccttcga
     2341 ccgcctgctt agaaggcagg tgctctatcc agctgagcta tgcgtccatt tgttttaaaa
     2401 cttatggaaa agttttggtc ggggtggcag gattcgaacc tgcggcctcc tgctcccaaa
     2461 gcaggcgcga taaccgagct acgctacacc ccgaagcgca aaaaaagcgg agagtaaggg
     2521 attcgaaccc ttggtacagt tccccatacg cttgtttagc aaacaagtcc tttcggccac
     2581 tcaggcaact ctccaatata agaacttata tttcttttaa gcggttgcaa atgtaagttt
     2641 ctattctata tttcacaaat aattaccctc tttttttaaa tgttttttca atagctattt
     2701 aaaatcatta acaatcaaac gcatagccaa ataaaaaata tacattttga atacatttta
     2761 attattctta ccaaataaca acctaaaaaa tcaataataa atatttaata ggcaaaagaa
     2821 tatattatta tcaaattgta atttagattt tactcaaatt gattaaattc gcatctcaaa
     2881 caatgcttat tcacaactca tgaacaaaaa agtcgttatt gttgctgctg tcagaactcc
     2941 aataggaagt tttatgggag gattatctac agtttctgct ccaaaactag gggctattgc
     3001 cataaaaggc gctttagatc gcatacaatt ggatgctagt ttagtcgatg aagtatttat
     3061 gggcaatgtc attcaagctg gagtaggtca agcaccagcc agacaagctg caatttttgc
     3121 tggcttaccc aattctgttg tttgcactac tgtcaataaa gtgtgcgcct ctggaatgaa
     3181 atcagtaatg ctggctgcac aagccattct ttgtggtgat gccgaaattg tggttgctgg
     3241 cggaatggaa aacatgagtt tgattccaca ttatttgcat ttaagaaatg gctataaatt
     3301 tggccctgct tcaatgattg acggaatgca gaaagacgga cttacagatg cctacgacaa
     3361 caatgccatg ggggtttgtg ctgatttatg tgctgacgaa tacaaaataa ctcgcgaaga
     3421 acaagacgcc ttcgccattc aatcctatga gcgttctgcg aaagcgtggg aagacggaaa
     3481 atttgaaaat gaaataattc cagttgccgt tgcgcaaaga aaaggagaac ctataatcgt
     3541 ttccaaagac gaagaattta ctgatgtaaa attagacaaa ataccttctt tgaatgccgt
     3601 ttttacaaaa gatggaactg taaccgctgc gaatgcttcg actataaatg atggagctgc
     3661 tgcaatgata ttaatgagtg aagaaaaagc aatttcattt ggtttaaaac cattagctta
     3721 cataaaaagt tatgctgatg ctgctcagga acccaaatgg ttcactacaa ctcctgctat
     3781 cgcgattaac aaagctttaa aaaaagtaga tttacctatt gatgctgtag attatttcga
     3841 atttaacgaa gcctttgctg tcgttggttt ggccaatgca aaaatccttg gtttagaaga
     3901 ttcaaaagtg aatgtaaatg gtggagctgt ttctttaggt catccgctgg gatgctctgg
     3961 tgctcgaatt atcgttacac tattgaatgt tttggagcaa aataatggta aaattggggc
     4021 tgccgcaatt tgtaatggcg gtggcggtgc ttcagcaata gttattgaaa gaatctaaaa
     4081 aaacaagtac aaaatatatg tttggaattt gcaatctagc cataatccct cttagaatcg
     4141 aacctagtga tcgtagtgaa attgtttcac aagttttgtt tggtgaacat tttgaaattt
     4201 tagaacagtt taaacaatgg tcgaaaataa aattacaatt cgataattat gaaggttggg
     4261 tcgattctaa acaataccaa attatttcag aatcagaatt tcatcaatta tcaaacgatg
     4321 tcattgttct aaatgctgat ttattggaat atattacagg gccaaacaat ttgttacttc
     4381 ctattccttt aggagcttcc ctttctttct tgactcattc agatataaat actgcaaatt
     4441 ttaactttga aggaacgaaa attagtggac taaaatctaa aaaaagttta ctaaatacgg
     4501 cctttatgta cctcaacgct ccttatcttt ggggtggtaa aactcctttt ggtgttgatt
     4561 gttcaggatt tacccaaatg gtttacaaac tcaatggcta taaattattg cgagatgctt
     4621 ctcagcaagc cttgcaagga gaagcgctga gttttataga agaaagcgag ccaggggatt
     4681 tagctttttt tgacaatgaa gaaggaaata ttattcacgt gggcattatt atgcaagaca
     4741 attatatcat tcacgccagc gggaaagtta gaattgaccg tttagaccat ctaggaattt
     4801 acaacgccga aaccaacaga catactcaca aacttcgtgt tatcaaaaaa attatataag
     4861 gtttaatagt caaaaatggt ttttaaaaat tgttattatt tttcttgaaa ttgataatct
     4921 gctacttcaa aacctgtggg tctaatgatt gaaaaatgat tttcattaat ctttatttta
     4981 aaggtttttt cctgacctgg ttctaattta tctatagtta ctttttcgtt tccagataca
     5041 attgaataat tttttaaatt ataacttggg atggcagttt tacctaatag cgtaatagtt
     5101 gcagtaccat tttttacccc aaccgattta ataataacag gggaactttc tttttttaac
     5161 gtgtaataag aaggctttgg atttaaaaaa atatcacaaa cgccatgaac tctttgcggg
     5221 taagcataag taaaatcttc tcccatatga gtacgataat cattaaggca gaaataaata
     5281 gcaccagcca catagtcttt tgagccataa atctttaatt gttctgtcat ttcttttatt
     5341 cgacgcagat ctcctccttt atgagcaggc tcacacaatc cccattctga aatagtcatc
     5401 gctttattgg gatattcagc cgaaatacga tctaattctc cgcctattac atccagactt
     5461 tttccaaacc aggtgctgta atattcattg aacatcatca tatcaaattc gcccgaagca
     5521 tccggaacat agtctttttg aattggtttt cctttctgta atttattgga aacataatta
     5581 accagccttg aagcatctat tgttttagct tcattgtaga gtgttttcaa cctatttaca
     5641 ttctcgggat tatggctttc taattcatta cctattcccc agcttattat ggatggatgg
     5701 ttgtaatgat tctcgatcat ttcttccaaa tgtttttttc ccaatgctag taaactgtca
     5761 ttaatcattg tagcaccacc ccaatatgga atctcttcct gaaccaaaat tccgttgcgg
     5821 tcacaccaat caaaaatggc ttcatcttgc tgccaatgaa aacgtgtaaa aatacaattg
     5881 gcattcttca tcagcttgag gtttctttca aaatcggcag ttgtttcagc cattccacgc
     5941 tccatattgg aacctggcat ccattctact cccattaagc ggattggttc tccgtttaaa
     6001 acatatctgt ttttttcaac tttaatactt ctaaaaccaa ataccgttgt gtattcatct
     6061 tttaacttgc cctctgcaaa tagctgaatc ttcaatttat acaaattagg agcgtcaaaa
     6121 tgccacggat taattttatc aaagtgtaag gaagttttaa acgcactttt ttctacagtt
     6181 ccttttaatt ctgaattaaa aataacctga ttagtgtttt ggttttcctc agtaatttca
     6241 accattaatt tcagcttcga aaaatctatt ttactgttat caataaaact aatatcaatg
     6301 tctgctttac ctttaagacc atttggtgtc ccaatcactt ttatgttttt tacggcttga
     6361 ggttccgtaa ttactaaaga tacacttctg taaattcctc catcattagc ccaatcgtag
     6421 ctctttaaaa aaggaatatt attgcttgaa aatgcattat tgacacgaac cgtaagcttg
     6481 ttaattcctt ttttgacaaa aggcgaaata gttataaaaa aacgattgta accggaacca
     6541 atatgctcac ctgctttctt gccattgata tacacaacag catcgtgata aacggcatca
     6601 aactgaacac gaaccgtttt ttgtaaatcg ctttcagtaa cttcaaattc acgctcatac
     6661 cagccgtttc cagcatactt ttctaatcct ttttcaacat tccaagtatg aggaacgcta
     6721 acaattcttg aggtattttt gggtaatcca tttatttccc aatgattcga ttctcctatt
     6781 gacaaagagt caacagaaaa gttccaattt ccatttaaag caattgtttt tcgtactgtt
     6841 tctttatcag ctttatgaaa agaagcaaaa aagaacacac aaattgataa cagaatgaac
     6901 cgaaatactt ttttcatatt gaagtttttt taataaatac ttttagccga ctctttggct
     6961 ttctcttttt cttctttcaa aatcaatttc aaaacctctg gattttcaat cgtatagggt
     7021 ttaaaggaag gaccgttcat ataatcaaga atcgaaacca acatctgctt agcaaccgga
     7081 cgtttatcca aatctttaga taaatcaaat gaagcgatta ccagttttcc tgaaccgatg
     7141 ctgccttcaa taagcgatgc cagtttacgg ttattggcaa aattatcgac catttcgacc
     7201 aaaggacttc cgcctttgat actgtcaaca attaaagtcg tggagtttac attcaaatcc
     7261 caccattgcc aatcggtatt catatcagta gggaaatcag caaaaacttt atgcttcgga
     7321 ttgcaaagta ctcccatagt tcctgcttgt tttggaaaat gaacaggact ccaaaaaacg
     7381 ggaacaaact tcccttcaat gccgcttaat tttttccaat ccgggtttaa caaaactttg
     7441 cgtccttgct gcaaggactt gtaggcttct tcaaggcttc gtgtataaac aacgtcttta
     7501 taatctattt tttgctgttc aggataaacc caaatattcc attggttttt ataatccgta
     7561 tcttttatgc ttactttaaa agttagttta gtcgcttttg taattttttg aagactttca
     7621 gcaaccttaa ataattcgtg attatagcct gaattgatag ttttagtcac tgatccagat
     7681 gcaattagaa tattattatc cacaatactc cactctatca ctttatcatt caaagcatca
     7741 cccgaaaaat tactcacatc caaacttgcc acaaaagatt cattgttcgt ataagtcgct
     7801 ttcggaaaac gcagcaaagg aacaaccggt gctgaaaatt gtctaaattc tgctgccgta
     7861 atcaaacctt tgctatccca aaaagcatcc agcaatccaa ccaatgccgt tccctgaccc
     7921 gggaaatcgt gtaaatctaa taactgaaat ccgctgattc cagccgtttt cattgctctt
     7981 tctatttctt ctttatagag aattacggct aattttcctg atgattttgt aaaatcggca
     8041 gccttattta ttaagctttt acgttctaaa tcctctttta tggaaataaa atttaatggt
     8101 tctaaaacac ccgtgtattt agcaatttca tcaattttag gataaacaga atactgacca
     8161 atttcgtggg taattaaagg taccgtgaaa ccttttaata cggctttgta atctttatcg
     8221 aatgttggag attcactgtt aaaaactcct tgtcctcgca cccagccttt gtttgtccat
     8281 tgtgtaataa aaaaatcatc ttctgcttca ggtaaagctc cgtgtccttt ttcaaatgta
     8341 aatgaagtac tggtatataa atgtctgttg tctttggctt taatattttt aaccatttcg
     8401 gtcaaaagtg ccatatcgcc ttgcaattca ttccccatcg accacaaaca aaacgaagga
     8461 tgattaccgt actcatccat catacgttgt gcttcagcat ataaaaagtc ggttacattt
     8521 ttatcttttc caatagttaa aacccaaaca gggagttcta cctgcaaata aaaacccatt
     8581 tcgtctgcca cttcaaaagc cgctttggga ggacaccagg aatgaaaacg aagatggttc
     8641 aatccccatt ccttcgccgt tttaaatact ttttcccagc cttttttgtc catcggagga
     8701 tgaccagtta acggaaaaat attgcattct aaagttcctc tcaaaaaaac agctttattg
     8761 ttatttgtta aaacagtttc gtttctggaa aactttcgca ttccaaaatc aactgacttt
     8821 tctgaattgt attttccggc agctaattga acttttaatt catacaattc aggagtaaat
     8881 tcactccaaa attgtacatc tttgcccatt tcataatcaa aatcgacaac agattgtcct
     8941 ttttccagtt gcactttttg ggtaaaagct gctattgatt ttttactttc tttatcaatt
     9001 gcaacgactg ttaaaactcc tttttgagat ttctttccat cattgttaaa actaacagat
     9061 accttaattt tgctcttatc aatattaggt tgcacttgta aattagcaat atgaaccgca
     9121 tcaatagctt caatattcat ttcgccaatg attccattcc aaataatttg agtttcattg
     9181 gtataagaat gcgccatatt gtctaagcta gtatcaaact gctttctgtt atcaacacgc
     9241 accgtaattc tttgtttttt acccggaatt aaataatgag ttaaatcaaa ataatgaggc
     9301 gaaaccaaac tgttttgtcc ttttgcaact tcttttccat ctatccaaac attggttttc
     9361 cagattacac gttctaattt caacagcaat tcttttcctt tccaatcatt cggaattaaa
     9421 acttcgcgag tgtaccaagc tgcgccgata taactattct ttctggtcaa atggctcaac
     9481 tgtggtttcg acaaagctgg cttcaatttg ttcggaactc ccataccaaa atcatcggtt
     9541 gttcccggca gattctccat ttgattaaaa cccatattct gccagccatt tttcaagcca
     9601 atatccgtac tgtccaattg tactgcccat tgaccagaaa tatcaattac ttttttttcc
     9661 aaggattgac tccacgaaca aattgaaaat gagacaacga ttaacaataa aatatttttc
     9721 atttgaatgt tttgataatt aaattttgaa ttgccggcag aataattatt ttctaaacgt
     9781 gtatttattg atgtctaagg ctgccacttt ttgataaaaa ctttctgaaa gctcctcatt
     9841 tcctaaaccg ctatgcccta atgccatcac gtaattgcaa tgtacctggt tgcgaatgtt
     9901 taaatcatct tcccaaatag ccaaatccgg aagtgacaca gcaaaataat caatacggct
     9961 tttttcaaat aaatactttt caccgtgagc cactaacttg ttaaaacgac tgcgggcttt
    10021 atcttcatca cctaattcgc gccaagccaa tccttgataa aaaatcttgt ccggctgttg
    10081 atcgttatag aaaaatgcct gaaccggttc tggagatcca agggttgctt ccataaaatt
    10141 ggctttggca ttttgctcat cgcctagccc tttataagcc attcctttgt aataatagac
    10201 ttcgttttct tcggcatttt ttagtttccc ttctcctaaa ttatgaggat aaacttctgt
    10261 ttctttcagt aattgaatgg catcaacaaa acgtttttct gtaattgcta ttttagctaa
    10321 ttcaactctg cataaagtat ataaacctgt tactttacct tcaccacctt cccaaggatg
    10381 gaattttcgg gaagctataa gatttttggc agtttcatac ttcccaattt gacaatacaa
    10441 accgatacgc tcaatacaca aatcgtctct ttcttctacc agactgctgt acttttctaa
    10501 aaacgctaat cgttcctctg gagatttccc cgttttttta tacaattgat ccaattccat
    10561 aaatattctg gaatcagttg tatccaatgc aaatgcctgc tccagaactt cctgtgagcg
    10621 gactttattt tctaatttat tataataagc aagggccagg tttcttaaaa ccgttggaaa
    10681 ctggttgtca attttcgcag aactttccca acattctatt gcattatcat actgacgaaa
    10741 tgcataccaa aaattcccta aataaaatgg tgctttggca tcattagtat tcaacaaaat
    10801 agcatcttgc agaatattta cttcttcaat tctatttgga aagcattttt ccggagataa
    10861 tgttgaagct tttttgtacc attcaatagc ttcgttttta tttcctttcc tggattcgaa
    10921 ataacctatt gtatagaaaa ccattggata aacagctgta acatcctgcg tacattcttt
    10981 taaaaactga atagcttctt catataatcc tgctccagca aaatcaagcg catattctat
    11041 atatccgtgc gaccatttgc gcataatggc attcatttca ttcagttcgg cttcatcatt
    11101 tgtcaacaaa tacttctcaa aacgacatcc catattaaaa cgatccatct taattgattc
    11161 attagctaaa gccaaagatt catcagttct tcctaatttt cttaaaatag aagtttttaa
    11221 ttggcgggct ttgtgattgt gccagttgcg tatcaaacaa gaatcgatgc attctaaagc
    11281 ttctttccat tctccacgga tacagtcaat ttgtgccaaa gcaaaaaagg cagcatcttg
    11341 ccaagcggca ttccaagtcg atttaaaaag tgctgtataa gcctcgtcca atttcccttg
    11401 aattttcaag caaaatccta aattataatg cggttctcca tcataaggat tcggactacg
    11461 ctgggttaaa acttctacag ccgttctaaa ataagcttcc gattttttaa attgtccgcg
    11521 tcttagcatc aataaaccaa ccgcattatt acaacgtaca tccttaggat ctctgcttaa
    11581 tgcttcttca taataatcta aagggttata agtagcgtga cgatattgct ccaagtgcaa
    11641 acctgttaaa aacaattgct caatcgaagc aatttcttta ggatcgagag ccgcttttgc
    11701 cggatctgga gctggtttaa tctccggttt ttcttcttga tattcaacaa gaacttttcc
    11761 gtcttcagtg taaatagtga aaatcaaatt ttcagaagct atattcccta taactacatt
    11821 ctttttataa ggattatatg gagaaatact aacaatttcc tgatgtaaaa tagttccgtc
    11881 aatgtctttt aattctaatt ttaaattttc gaaaagactg gtggtaaaca aaattaaatc
    11941 ggctctatca ccatctattt caatgttgat tatagcatct ttggtagcat ttttaacata
    12001 acccacttcg gcataaggca taaaatactg cacccaagat ttctcttcat aggcttgaag
    12061 ccaagaaaaa tcaggttgat tatccgtaaa aaccccggtc attaattcaa tgtaagggcc
    12121 gtctttatcc gtcaaatttc tgtcccaggc cagaccaaaa tcgccattac cccaagtcca
    12181 ttgtttttta cccggtgaaa cgtgatgatt ggcaacgtgc agcaaacctg cttgcacccc
    12241 atcttcataa ccacccacaa aattgtattt agaatgcacc gccatataag aggttggcac
    12301 aggaatattt ttatatttcg aaatatctac tcctgctgaa taatcttgct tgtaatattc
    12361 cccagttgct atcgggaaat tagaaacatc ccgttttccg tggtcgaata cgaaatttac
    12421 atctggtgga aaaaccgact tgtattcgtc attaaccact acggctggat ttgcccacca
    12481 aagaaaagtt tgcggaaaag ctgttctgtt ataaattttg acttttattt caatataagc
    12541 cttatcagga tgcagcgtga atccctgcat tcctttggtt ctaaacattc tttctacttc
    12601 attacaccaa acggttttac taccatcggc attttcttcg atgctaaaat cggtaggtaa
    12661 aaaagtactt ggacgatggt gctgaggcca gttgaactca attcctcctg aaatccaagg
    12721 tcctgtcaat ccaaccaagg ctggctttat tacctgattg taatacacaa aatgacgctg
    12781 ttttaccttg tcataagcca tatgaatacg accgcctaac tctggcaaaa tcattatttt
    12841 gatgtattta ttttctacaa acaaagcgtg gtataattta tctttcttta catccgatat
    12901 tttttcgaca acaggataag gataaacgga acctgaactt ccctgataca ctcttttctc
    12961 caaaaaaata ggattttttt cttcttcacc aatttcataa gtcggcaaca taatatcctc
    13021 ttcccatgca cgaactactc catcctcatt tactgtagat tgaatcaaat aagccgtaat
    13081 aggctcgtct gctatttctg taattatatc tgattggact gaaattaatt tttccatctt
    13141 aattatgtta tgcttttatt attttgttaa ttctgattct atttgttcta atgatttccc
    13201 ttttgtttcc ggcagataaa ttcgtaaaaa aagatacccc agaaaacaaa ttataccgta
    13261 aagccaaaag gttccataag ctcctaaacc agtatttaat aaaggaaaag tataggtaag
    13321 cacaaagcaa gctgtccata atgaaaaggt acttacagcc attgccattg ctctaatttt
    13381 tacaggaaat atttcagata aaacaaccca ggtcactgga gccaatgtca ttgcatagca
    13441 agcaatggca agaattacaa gaattaaaac tgccgaacct gttacagcaa aataataaca
    13501 gatacccaaa agggcgtaaa tacaagccag tccgatggaa ccaaaaagca tcaaagctct
    13561 tctgcctaat ttatctacca gcaccattcc aacataagta aatataagat tggtcaaacc
    13621 tgtaataacg atattgaata gaatatcaga aacaccataa ccagcagcag aaaatatttc
    13681 ctgcgcataa ttaaaaacca cattaatacc gcaccattgc tgaaaagcag cgataacaat
    13741 acccagcaaa agaattttag gcattttgcc ttcgaataac attttataac tagctgcttc
    13801 ttttacaata ctaactgtag tttttaattc ttcgatttgt tccgtagcaa attgaagccc
    13861 tcctattttt gtaaaaatag attgtgcttt ttcgtatttg tattgactgg ctaaccatcg
    13921 tggactttct ggaattatta atattaaagc aaagaaaact atcgcaggaa ttcctccagc
    13981 ccaaaacata tacctccagc cccattgtac attccaggaa tccattgcaa tacctccgtc
    14041 aactatagga tctgctaaaa gcatattcac aatttgtgca gccaaaatac caacaactat
    14101 agtcaattga ttaattgaaa caaatttgcc tcttgattca ctcggcgcta tttcggctat
    14161 atacattggt gaaagattag aggctatacc gatacccact cctcctatta ttctccaaac
    14221 aataaacata ttgatactat ttgctaatcc tgttcccaca gctgccaatg tgaataaaat
    14281 agcagccaaa accattaatg gttttctacc atatttatca gacatacttc ctgaagccat
    14341 tactcctaat aaacatccta aaatagcact tcccatcacc cagccttgca tagcgggaga
    14401 atccgttata tggaaataaa cttcataaaa tggctttgca ccaccaataa ctacccagtc
    14461 atacccaaac aagagaccgc ccaaagcaga aacaatggca ataaacaaca aataggaaga
    14521 attactttta ttcataattt tagaatttac attttaataa tgacaaattt acatcgagaa
    14581 aggatgttga aaaatgcaaa aaacaaatga aaagatgtat ataaaaacta ttttacacaa
    14641 caaatttcta tattttaatt taaaatacta ttttgattat tccaaaataa tatttttcat
    14701 agtgtttttt tttaatatta aattccacat aaataactaa ttttgaataa taataatttt
    14761 aaaaaacaca ttttattata ttcaacctat taaaaaggat aattaaatac caatgattca
    14821 aattcaaaaa cctgtatttt ttaacttatt tattgcaaat ataaattttc aataaatgga
    14881 acatatcgct gatggtttta aaggagaaag agcaattgta ttgccttaca gcatccgaaa
    14941 ttttcaaacc aataacgaac taactaaatc actttatgtt acccacattg gttattaccc
    15001 caatgccaaa aatcattttc gcaaacgttc caaggagctc ttgaagacat tttgatttat
    15061 tgcgaaaagg gaaaaggaac tataacctta gaaaatgaaa aattcaatct gacagaaaat
    15121 caaattttca ttattcctgc aaacaaaacg cactcctatg aatcagatcc tttaaaaccg
    15181 tggagtatct attggatcca ttttaaaggc acgaaagctt tatattatgc tccgaataaa
    15241 ggaaaaataa taaccctaaa tgattcagga atatcaaatc gatttgaact ttttgaagaa
    15301 atttatcaaa atttagaaat gggattttat gcagataatc tagagtactc aagcatttgt
    15361 ttgaaacatt ttctttctag catcaaatac attgaccagt ttaaccgaat aaaatcttta
    15421 aacacctctg atttagatac aattcctaag agtattattt ttatgagaga caacttgaca
    15481 aacaaaatat ctttaagcca aattgcccaa tatgtgggtt attcggattc ttattttaga
    15541 tccttatttt tagaaaaaac atcttttact cctattgaat atttaaacaa actaaaaatc
    15601 caaaagtctt gttcattatt gcaatttagt actatgaaaa taaatgaaat tgcctatcat
    15661 ttagggtatt ttgatccatt tcatttttca aaatctttta aaaaggaaat ggaaatatcg
    15721 ccccgtgagt atcgaaaaaa acataaaaat tagacaaaaa aaatcccgat ttcaccaatc
    15781 gggattttca tttaagaatt aacctttacc tatattctta ttttttatta gcttctaatt
    15841 cttttacttt tacttttaga gctttatatt caggcatcat ttctaatgcg ctataaacat
    15901 ttaaaagtgt tttagacaca tcaacatttg ttggttctaa ctcaactgct ttttgaagat
    15961 acggaatgat tcctttaaac aaatcttctc ttttaacttt caacacatca taacgtttca
    16021 tgtctttggc agaagttcct aatttgttca tttcatcaat aattaccttc tcatcttcta
    16081 atttcaaagc agcataattg atataagcat tggcatattt aggattaatt tccattactc
    16141 ttttataata cttttcagcg tctgcaatat ttttcgcatt tgcacttaac acacctaaat
    16201 taaatactaa atcggcatca tttggatttt tttctaaagc ctcagcaact aattttttat
    16261 aagtctcata atcttttgtt t
//
LOCUS       DAOSNN010000025        16110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3655_length_16110_cov_5.184678, whole genome shotgun sequence.
ACCESSION   DAOSNN010000025 DAOSNN010000000
VERSION     DAOSNN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 16110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16110
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3655_length_16110_cov_5.184678"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLQ70_02465"
     CDS             complement(<1..397)
                     /locus_tag="PLQ70_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQE33504.1"
                     /translation="MERTDYKQEINMTEALMTRINELISHYPEGKQKSALLPVLHEVQ
                     DAHENWLSIELQDKVAEILSIKPVEVYEVVSFYTMFNRRPIGKYMFEFCQTSPCCLNG
                     VEDLMDYTCEKLGVGIGETTPDGLFEVRGV"
     gene            complement(471..1709)
                     /locus_tag="PLQ70_02470"
     CDS             complement(471..1709)
                     /locus_tag="PLQ70_02470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQE33505.1"
                     /translation="MSELLLPPEHRYAKIIQERHNEDGSELSILNLGPTHPATHGIFQ
                     NILLMDGEKIIEAEPTVGYIHRAFEKIAENRPFYQITPLTDRMNYCSSPINNMGWWMT
                     LEKLLDIEVPKRVQYLRVIVMELARITDHLICNSILGVDTGAYTGFLYVFQFREKVYE
                     IYEEICGACLTTNMGRLGGFERDWSPEAFRKLDVFLKDFPVAWKEFENLFERNRIFID
                     RTVNVGPITAEMAMAYGFTGPNLRAAGVDYDVRVAHPYSSYEDFDFIVPVGKSGDTYD
                     RFCVRNAEIWESLSIIRQALDKMPEGNVYHADVPEYYLPPKEDVYHDMESLIYHFKIV
                     MGEIPVPVAEVYHAVEGGNGEVGFYLVTDGSRTPYRLHFRRPCFIYYQAYPEMIKGAM
                     LSDAIVILSSLNVIAGELDA"
     gene            complement(1760..2281)
                     /locus_tag="PLQ70_02475"
     CDS             complement(1760..2281)
                     /locus_tag="PLQ70_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQE33506.1"
                     /translation="MSLESTTVQDKLVETFGESVFQFNQEKDVFSFEVAADKITAVVL
                     FLKNDPTLRFHFLTDICGVHYPDNAVERQFAVVYHMHNWYENKRIKIKAFINGETPEI
                     KSLTTIFPGANWMERETYDFYGIIFTGHPQLKRILNMDEMVSHPMRKEFPMEDGGRTD
                     KDDRFFGRTAFNG"
     gene            complement(2286..2834)
                     /locus_tag="PLQ70_02480"
     CDS             complement(2286..2834)
                     /locus_tag="PLQ70_02480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HQE33507.1"
                     /translation="MSDSNIKMVAPPEGVSGEGFFATTLNDVVGLARANSLWPLPFAT
                     SCCGIEFMATMASHYDLARFGSERVSFSPRQADMLLVMGTISKKMGPILRQVYEQMAE
                     PRWVIAVGACASSGGVFDTYSVLQGIDKVIPVDVYVPGCPPRPEQIVDGVMRLQELVK
                     SESVRRRSSPEYQELLASYNIK"
     gene            complement(2954..3319)
                     /locus_tag="PLQ70_02485"
     CDS             complement(2954..3319)
                     /locus_tag="PLQ70_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQE33508.1"
                     /translation="MQSSQLDYLPILMQMLLAVGFVVAIIIISGKLGPKRSSKIKDTN
                     FECGIESIGNARIPFSVKFFVVAILFVLFDIEVVFLYPWAVNFKELGLEGMIKMVVFM
                     ALLLIGFFYIIKKKALDWE"
     gene            complement(3529..3723)
                     /locus_tag="PLQ70_02490"
     CDS             complement(3529..3723)
                     /locus_tag="PLQ70_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HQE33509.1"
                     /translation="MRTGTVKFFNESKGYGFITDEETGKDIFVHASGINAEELREGDR
                     VSYEEEEGRKGKVAAKVAVI"
     gene            complement(3852..5600)
                     /gene="aspS"
                     /locus_tag="PLQ70_02495"
     CDS             complement(3852..5600)
                     /gene="aspS"
                     /locus_tag="PLQ70_02495"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQE33510.1"
                     /translation="MYRSHNCGELNASNINQEVTLAGWVQKSRDKGFMNWVDLRDRYG
                     VTQLIFDESRSQKEVFELAKTLGREFVIQAKGIVIEREAKNPNMSTGDIEILVTELKI
                     LNSSITPPFTIEDETDGGEDIRMKYRYLDIRRNPVKNNLLFRHKVAMEVRKYLSDLDF
                     CEVETPYLIKSTPEGARDFVVPSRMNEGQFYALPQSPQTFKQLLMVGGMDKYFQIVKC
                     FRDEDLRADRQPEFTQIDCEMAFVEQEDILNVFEGLTRHLLKEIKGIEVEKFPRITYE
                     YAMKTYGNDKPDIRFGMEFGELNEFAQHKDFSVFNSAELVVGIAVPGAGNYTRKEIDG
                     LIDWIKRPQIGASGMVYVKCNEDGTFKSSVDKFYDQDDLTNWSKATGAQSGDMIFVLS
                     GPADKTRTQLSALRMELAARLGLRKSDEFAPLWVVDFPLLELDEESGRYHAMHHPFTS
                     PKPEDMSLLETEPGKVRANAYDMVLNGNEIGGGSIRIHDKATQQLMFKYLGFTPEQAE
                     DQFGFLMNAFQYGAPPHGGLAFGLDRLVSILGGQETIRDFIAFPKNNSGRDVMIDAPS
                     VIDELQLNELHIKLDL"
     gene            5756..6448
                     /locus_tag="PLQ70_02500"
     CDS             5756..6448
                     /locus_tag="PLQ70_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33511.1"
                     /translation="MKNSILLTFLVCFQIGIAQEKWFTTYSDTISLVNDAQLITKSFT
                     KDIIKINKTIKYDIKTVLHTTPYLIYFYKDAANIPFWDQVIPQQKQFFYEIAGSEEEG
                     KKIFGLFFNGFYLPHELGHAFEFVLKGQIVNSYQSEYFANTVAILWWKKHHRDTELKQ
                     CYEAAKKIWAKLPNPVPAGSTIEEYFSKNYEQASQNPYVYGYMQFKQFIEIYENQNLP
                     DFDTFIKQQSKK"
     gene            complement(6860..7993)
                     /locus_tag="PLQ70_02505"
     CDS             complement(6860..7993)
                     /locus_tag="PLQ70_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HQE33512.1"
                     /translation="MNKKVFVSGCYDMLHSGHIAFFEEAAQQGDLYVGIGSDKTINEL
                     KARKTINSDAERLYMVNSLKVVKDAWINRGSGLLDFLEELHTLKPDIFFVNTDGHTPQ
                     KEALCKEMNIEYIVSKRIPHGNLPTRSTTALRQECRIPYRIDIAGGWLDQPYVSKYVS
                     GSVITICVEPDYEFNDRSGMSTSSRKKAIELWHTDIPEGEKEKLAKTLFCFENPPGTK
                     YVSGSQDALGITMPGLNKYYYDGEYWPSDIQSVMVSELLDWIEQRIWMIPLYPREMDY
                     DVLANTQIDEKGARWLSEATDACWKALQQQDAPAVGKAMTQSFEAQISMFPNMVTPDI
                     MAQIDSHKKDVLGWKVSGAGGGGYLIFFSEKSIENAIQIRIRR"
     gene            complement(8364..9272)
                     /gene="lepB"
                     /locus_tag="PLQ70_02510"
     CDS             complement(8364..9272)
                     /gene="lepB"
                     /locus_tag="PLQ70_02510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQE33513.1"
                     /translation="MNSEINSEKIKPRNPKLAFLLALFGPGFGQIYNGQIKKGIIFLT
                     IELLLPVFYGLTKLGVFFAGFISILVIGFLYRIYLIYDATTNAKKLKNFKPKSYNTWY
                     YFLGIIVGIYTILWFYDTNSIVGVKSFKIPTTSNEPAIKVGDRIIADLNAFDNIKPNY
                     GDIVIFQKKDSLHPWIYRIVGLPNDRLEIQNNFLIINDKKCKTSFIKETKSEEFDVKE
                     YEEELPNGHKHKIYTFKKPFEDDENAEHEIIIPKNSYFLMGDNRDNAMDSRYIGVLKE
                     DEIKGKVVFSYWGKTNDRINIDFRNK"
     gene            complement(9396..9800)
                     /locus_tag="PLQ70_02515"
     CDS             complement(9396..9800)
                     /locus_tag="PLQ70_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQE33514.1"
                     /translation="MQYLLDTNICVFFLRGKLNLDEIIRDKNPENCFVSEITVFELRY
                     GAENSENPTKSHKAVDLFINGITVIPIYSSIKKYAKEKVRLRKIGKPMHDEFDLLIGV
                     TAIENKLTLVTDNEKDFVNIDGIEIENWFVRK"
     gene            complement(9800..10039)
                     /locus_tag="PLQ70_02520"
     CDS             complement(9800..10039)
                     /locus_tag="PLQ70_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33515.1"
                     /translation="MSYADKHIVETYSMLFDGLSSLSKIELIEKLTKSLKREKTAKKD
                     TFFEAFGAFSDEKSAEEIIADLKLSRKFRNKEIKF"
     gene            complement(10248..13418)
                     /locus_tag="PLQ70_02525"
     CDS             complement(10248..13418)
                     /locus_tag="PLQ70_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQE33516.1"
                     /translation="MKIVDISIKRPSIVIVLFTILLLGGLFSYQQLSYELIPKFEVNV
                     VTVSTVYPGASPGEVENTVTKKIEDAISTMENIKKIESKSYESLSVVMITLTTEADAD
                     YALNDAQRKINAVLKDLPDDVDPPSLSKFSLSDLPIMTIGATGKMDEIAFYDLLDKKI
                     QPILSRVPGVAQVNLVGGQEREIKVSLDSKKLQGYGLSIPQVQQAVLSSNLDFPTGNI
                     KTRENKTQIRLSGKYKSVEEMRNLVISSNKGVQIRLGDIADVQDAQKDVEKISRVNQK
                     SSIILQVIKQSDANAVEVSAKVKKAIVQIEKDYTKSNLKLAVANDSSEFTLTAADSVM
                     HDLFIAIILVAFVMLFFLHSIRNALIVMVSIPASLIATFIGIYLMGYTLNLMSLLGLS
                     LVVGILVDDAIVVLENIHRHMEMGKNKVRAAYDGAAEIGFTVMAITLVIIVVFLPIAM
                     STGLVSNIITQFCVTVIIATLFSLLASFTIVPWLFSRYGKLEHITKKTFFGRVILGFE
                     SYLDVFTHKVTDILRWSLVYKKTTLAIVSILFLASTMGLVGGGFIGGEFFAKTDKGEF
                     LVQIEMPKDASVEQTNFMTQKAEEFLRSDKNVVDLITTVGQTSEGMGATQATSYKAEI
                     LVSLVEKKEREDNSFIFAAKIKRELEKVLVGAKVKTVPMGLLGADQAPIKLTVTGPNF
                     EDAMAFATKAAAELKKIPGSSEVKLTSEAGNPEIKVVVNRDKMAALGLTLQTVGMTMQ
                     TAFSGNTDGKFRAGEYEYDINIRFNEYDRSNAKDVNDLIFLNSTGQQIKLSQFADVIE
                     SSGPSMLERRDKSTAVSIEAQTVGRPSGTVATEWETVFSKMERPAGVNYVWGGDMENQ
                     TEGFGTLGIALLAAIILVYLVMVALYDDFVTPFVVLFSIPLSFIGALLALALTNNSLN
                     IFTILGIIMLIGLVCKNAILLVDFANHRKDAGESTFDALVQANHARLRPILMTTIAMV
                     IGMIPIALAKGAAAEMNNGLAWVVIGGLMSSLFLTLIVVPVVYAIFDSIRVRLGKDQK
                     VDYAELMTAEYKHRKLVDGFNPKHKM"
     gene            complement(13437..14504)
                     /locus_tag="PLQ70_02530"
     CDS             complement(13437..14504)
                     /locus_tag="PLQ70_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQE33517.1"
                     /translation="MKKPIYIIIAVVLIGGAAFTLINNKKKNAEDTAIVAQKNSSVAV
                     RVATVTTGKLDDAFKANGNFEPIQELTFSAEHSGKVIKVLAKEGDYVNVGQPLLIVRG
                     DVINISAQAAKAAYDNSKADYARYENAYKTGGVTKQQLDQARLALTNAESNWKQANIN
                     VGDTKVKAPIKGYINKKYVEPGSILTGMPATALFDIVNVSKLKLKVTVNESQVAGLKL
                     GNNVSISASVFPDKSFTGKITFIASKADESLNFPIEIEVANNPNNEIKAGMYGTASFE
                     SQAAKQTNVKSIPRTAFVGSVSSNQVFVINNNMATLKKIVAGRIFGDRVEILDGLNDG
                     EVVVTSGQINLSDNTKVNIVK"
     gene            complement(14515..15849)
                     /locus_tag="PLQ70_02535"
     CDS             complement(14515..15849)
                     /locus_tag="PLQ70_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE33518.1"
                     /translation="MKKLFILPLLILGLTASAQVQTLTLKEAITYALKNKADAQKSRL
                     DMENSEYKIQEVRSRALPQISANGSLTYNPILQTNVLDGAMFGQPGQSVQVSFGQKWT
                     SGAGVSLSQAIFDQSVFTGLRAANSTREFYQINNQLTEEQVIERVANNYYSVYVQRER
                     LMLLDSNYVNTTKVRDIVQGQFDNGLAKKIDLDRIIVKMSNIDTDRQQIKNQIELQEN
                     SLKFYMGMPIETQISMPKTEFEVTPIALTEAPSTEKRTEYLLLKKQEELLEFQKTSIK
                     AEYYPTLSLVAGYNYLGQGPEMPLFAKPADGVYWSDYSSIGLNLKVPIFTGFGTRSRV
                     RQADNAIKSLQVDIKDTQLALDLDFRNAKAQIENNLVTIKNQKENMRLAGDILKNTKN
                     NYLQGLASLTDLLDSENASLEAQNNYTRAILAYKIAEIALIKSKGELKNLLN"
     gene            complement(15849..>16110)
                     /locus_tag="PLQ70_02540"
     CDS             complement(15849..>16110)
                     /locus_tag="PLQ70_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQE33519.1"
                     /translation="RELNECNAVFKQNIVKGIEQGLYRKETPIDTYVEFYYTLIFSIN
                     GKISSEKEANEKELLALEYHTRAMATPEGIIELEKQLINYHL"
BASE COUNT         4852 a         3061 c         2610 g         5587 t
ORIGIN      
        1 ctacgcctct tacttcaaaa agtccgtctg gagtagtttc gccaattcca acgcctaatt
       61 tttcgcaggt atagtccatt aaatcttcaa caccattcaa acaacaaggc gaagtttgac
      121 aaaattcaaa catatattta ccaattggtc gtctgttgaa cattgtataa aaggaaacta
      181 cctcatagac ttcgacaggt tttatcgaaa gaatttcggc tactttgtct tgcaattcaa
      241 tgcttaacca gttctcgtgt gcatcctgaa cttcgtgtaa aacaggcaat aaagccgatt
      301 tttgtttccc ttcgggataa tgactgatta attcattgat gcgagtcatc aatgcttcag
      361 tcatatttat ttcttgtttg taatctgttc tttccattct atattttaga tttagattta
      421 gatttagatt tttcaatctg cattctaaaa tctgtattct gcaatctttt ttaagcgtct
      481 aattctcctg caataacatt taaactggat aaaatcacaa tcgcatccga aagcattgcg
      541 ccttttatca tttcaggata agcttggtaa taaataaaac aaggtctgcg gaagtgtaat
      601 ctataaggag ttctacttcc atcggttact agataaaaac ctacttctcc atttccgcct
      661 tccacagcgt gatagacttc ggcaactgga acaggaattt ctcccattac aatcttgaag
      721 tggtaaatca aagattccat atcgtgataa acgtcttctt ttggaggcaa ataatattca
      781 ggaacatcag cgtgatatac atttccttca ggcattttgt ctaatgcttg acgaatgatg
      841 ctcaagcttt cccaaatttc ggcattacga acacaaaaac ggtcgtaagt atctcctgat
      901 ttcccaacag gcactatgaa atcgaaatct tcataagaag agtaaggatg cgccacacga
      961 acatcataat ctacaccggc agcacgtaaa tttggacctg taaatccgta agccattgcc
     1021 atttcggctg taattggacc tacatttacg gttctgtcaa taaaaattct atttctttcg
     1081 aataagtttt caaattcttt ccaagccact ggaaaatctt tcaaaaacac atctaattta
     1141 cggaaagctt ctggtgacca atctctttcg aaaccgccta atcttcccat attagtagtt
     1201 aaacaagctc cacaaatttc ttcgtagatt tcatagactt tctctctgaa ctgaaaaaca
     1261 tacaagaaac cagtataagc tcctgtatct acaccaagaa tcgaattaca aatcaagtgg
     1321 tctgtaattc tagccaattc catcacaata actctcaggt attgtactct tttcggaact
     1381 tcaatatcca ataatttttc caatgtcatc caccatccca tattattgat aggagacgaa
     1441 caatagttca ttcggtctgt tagtggagtg atttgataaa aaggacggtt ttcggcgatt
     1501 ttctcaaaag ctctgtggat atatccaacg gttggctctg cttctataat tttctcgcca
     1561 tccatcaaca agatattttg aaaaataccg tgagtcgctg ggtgcgttgg tcctaaattc
     1621 agaattgaaa gttcgcttcc gtcttcgtta tgacgctctt gaattatttt agcatatcga
     1681 tgctctggtg gtaataatag ttctgacata aaaaattgat aattgataat tgatttaatt
     1741 atcaattgtt aattgttaat tatccattaa aagctgttct tccgaagaaa cggtcgtctt
     1801 tatcagttct tcctccatct tccatcggga attcttttcg cattggatgc gataccattt
     1861 catccatatt taaaatacgt ttcaactgcg gatgacccgt aaaaatgatt ccataaaaat
     1921 cataagtttc tctttccatc caattggcac ctggaaatat ggttgtcaaa gattttattt
     1981 ctggtgtttc tccattgata aaagctttta ttttaatacg tttgttttca taccagttat
     2041 gcatatgata gactactgcg aattgtcttt cgacagcatt atccggataa tgaacaccgc
     2101 aaatatcggt taggaaatga aaacgcaatg tagggtcatt tttcaagaaa agaactactg
     2161 cagtaatttt gtctgctgct acttcaaatg aaaaaacatc tttttcctga ttgaattgga
     2221 aaacactttc tccaaatgtt tccactaatt tatcttgaac ggttgtcgat tctaaactca
     2281 ttttcttatt ttatgttata agaagctagt aattcctgat attcaggaga acttcttcgt
     2341 cttaccgatt cactttttac taattcttgc aatctcatta caccgtccac aatttgttct
     2401 ggtcttggcg gacaacccgg aacgtaaacg tcaactggga ttactttatc tattccttgc
     2461 aaaacagaat aggtatcaaa aacaccacca gatgaagcac aagctccaac agcaattacc
     2521 catcttggtt ctgccatttg ttcgtaaact tgacgcaaga ttggccccat ttttttggaa
     2581 atggttccca taaccaacaa catatcggct tgacgtggag aaaaactcac acgctcagaa
     2641 ccaaaacgcg ctaaatcata atgagatgcc attgtagcca taaattcgat accacaacaa
     2701 gaagtggcaa aaggcaatgg ccaaagcgaa ttagcacgag ccaatcctac aacatcattt
     2761 agcgtagtag caaaaaaacc ttcgccagaa acgccttcag gaggggcaac catttttata
     2821 tttgaatcac tcattttttt aaaattatga attatgaatt atgaattatg agttcacaat
     2881 ttaaaatcta agatgttttt agtaaatata tttagaaaat agaattcgta atttctaaat
     2941 ttcaaattga tttttattcc cagtctaatg ctttcttttt gatgatatag aaaaatccaa
     3001 tcaacaacaa agccataaaa acgaccatct taatcattcc ttctaatcct aattctttga
     3061 aattgacagc ccagggataa agaaaaacga cttcaatatc aaatagtaca aaaagaatgg
     3121 ctaccacaaa aaatttaaca gaaaagggaa ttctagcatt cccgatagat tcaatcccac
     3181 attcaaaatt ggtatctttg atttttgagg atctttttgg gcctaatttt ccagaaataa
     3241 taattattgc cacaacaaat cctacagcta atagcatttg cattagtata ggtaaataat
     3301 ctaattgact tgattgcata aataatattg gtttagtcta aataagccac aaagataggc
     3361 ttcgtgtctt taaaacacaa gttaaatgca aaaatttagc aaaaggaaat acaatatcgt
     3421 tttcggaaaa acattccaat attcaagtag gctataaaac aaaaaagcgt cccgaattac
     3481 ttcgggacgc tttaatcctt cttagccaat aaaaatgaat ttttatgctt agattactgc
     3541 tactttcgca gcaacttttc cttttcttcc ttcttcttct tcatagctca ctctgtcacc
     3601 ttcacgaagt tcttccgcgt tgattcctga tgcatgaaca aaaatgtctt ttcctgtttc
     3661 ttcgtctgta atgaatccgt aacctttaga ttcattgaaa aatttaactg tacctgtacg
     3721 cattgtaata gtaataataa tttataatgt ggcaaatgta atatttaatc taatacgaaa
     3781 gccgatattg caaaaatttt attaaaatat ttgatattca acgatttcta acctatttta
     3841 tcgataaacg gctataaatc taatttaatg tgcaattcgt tcagttgcaa ttcatcaatt
     3901 accgagggag catcaatcat cacatctctt cctgaattat ttttcggaaa agcaataaaa
     3961 tcacggatgg tttcctgacc tccaaggatt gaaactaatc tatccaaacc aaaagctaag
     4021 cctccgtgag gtggtgcacc atattgaaaa gcattcatca agaatccaaa ctgatcttcg
     4081 gcttgttcag gtgtgaatcc taaatattta aacatcaatt gttgtgttgc cttatcgtga
     4141 atacgaatcg aaccaccacc aatttcgttt ccattcagga ccatatcata agcattggca
     4201 cgaacttttc cgggttcagt ttctaacaaa ctcatatctt ctggttttgg cgatgtaaac
     4261 ggatgatgca tagcatgata acgaccgctt tcttcatcca attctaataa cggaaaatca
     4321 accacccaaa gtggcgcaaa ttcatctgat tttctcaaac ctaaacgagc agctaattcc
     4381 attcgtaaag ccgaaagttg ggttctagtt ttgtctgctg gtcccgaaag tacaaaaatc
     4441 atatctccag attgtgctcc tgttgctttt gaccaattag ttaaatcatc ttgatcatag
     4501 aatttatcta ccgatgattt aaaagtgcca tcttcgttgc attttacata aaccattccg
     4561 ctggcgccaa tttgtggacg tttgatccaa tcaattaagc cgtcgatttc tttacgagtg
     4621 taatttcctg ctcctggaac agcgattcca accaccaatt cagccgaatt gaaaacagaa
     4681 aaatctttgt gttgtgcaaa ttcattcaac tcgccaaatt ccattccgaa acgaatatct
     4741 ggtttgtcgt ttccataagt tttcatggca tattcgtagg taattcttgg gaatttttct
     4801 acttcaattc ctttgatctc tttcaataaa tgacgggtca atccttcgaa aacattcaga
     4861 atatcttctt gttctacaaa agccatttcg caatcgattt gtgtaaactc tggctgacgg
     4921 tcggcacgca aatcttcgtc acggaaacat tttacgattt ggaaatattt atccattccg
     4981 cccaccatca ataattgttt gaacgtttgt ggcgattgtg gcaatgcata aaattgccct
     5041 tcgttcattc ttgatggcac cacaaaatct ctagcgcctt caggagtcga tttgattaaa
     5101 taaggggttt ctacttcaca aaaatctaaa tccgaaaggt attttcgaac ttccatggcc
     5161 actttatgac gaaaaagtaa attgtttttt acaggattac gacgaatatc gaggtaacgg
     5221 tatttcattc gaatatcttc gccgccatcc gtttcatctt cgattgtaaa aggtggcgtt
     5281 attgatgaat ttaatatttt gagttcagtt actagaattt ctatgtctcc tgtggacata
     5341 tttgggtttt ttgcttcgcg ttcgataaca atccctttgg cttgaattac aaattcacgt
     5401 cctaaggttt tggccaattc aaaaacttct ttttgagagc ggctttcgtc aaaaattaat
     5461 tgtgtaactc cgtaacggtc acgcaaatcg acccaattca taaatccttt atctcttgat
     5521 ttttgaaccc atccagcaag tgtaacttct tgattaatat ttgaggcgtt gagttctccg
     5581 cagttatgac ttctatacat attctaaatt ttagattgca aaagtaaaaa caaaaaacaa
     5641 cttaatctct aaaactgtga acttcattaa agattattct tcaaattatt atcattagta
     5701 cagagtttca taaaataaat tatatttgac tttgtgaaat ttaaaatcta accctatgaa
     5761 aaatagtatt cttctaactt ttttagtttg ttttcaaata ggaattgccc aagaaaaatg
     5821 gtttaccact tatagcgata ctatttcact tgtaaatgat gctcaattaa taaccaaatc
     5881 tttcactaag gatataatta aaataaacaa aaccattaaa tacgatataa aaacagtatt
     5941 gcatactact ccttatctta tttattttta taaagatgct gccaacattc ctttttggga
     6001 tcaggtgatt ccacagcaaa aacaattttt ttatgaaatt gccggaagtg aagaagaagg
     6061 aaaaaaaatc tttgggttat tttttaacgg attttatttg cctcacgaat tgggtcacgc
     6121 atttgagttt gtattgaagg gacaaattgt aaattcttat caaagcgaat attttgccaa
     6181 tactgttgcc attttgtggt ggaaaaaaca ccatcgtgac acagaattaa aacaatgtta
     6241 tgaagctgcc aaaaaaatat gggccaaatt accaaatcca gttcctgcag gctcaaccat
     6301 tgaggaatat tttagtaaaa attatgagca ggcttcacaa aatccttatg tttatggcta
     6361 catgcagttc aaacaattta tcgaaattta tgaaaaccaa aacctacccg attttgacac
     6421 attcattaag cagcaatcca aaaaataatt tactttaaaa gttttcttaa cgaaaattgt
     6481 aagtaataaa acttgaaatt tattgactag cgaatgaagt ttttgaagat gtaatgatga
     6541 ttcaaaacag tgaattcact tttttgaaag taggttgtaa gacaaatatt gacaaaaaat
     6601 taatattgct ttatttggga ttgaatccgt caatttcttt gtaaaatatt ttttttgtaa
     6661 taagtatttt tttaaattta ctacatagaa agacgttcat gatttcagaa attcgagata
     6721 aaaatttaga caataaattt ctctttctgt gtattaaatc aatacttata gcgtctataa
     6781 aaaaatcggc tgtcacaaaa atttgtaaca gccgattttg attttataaa tggttaattt
     6841 ttcttgattt caaaaaaaat taccttctaa ttcgtatttg aattgcattt tcaatggatt
     6901 tttcactaaa gaaaatcaaa taacctcctc caccagcgcc actaactttc caacccaaaa
     6961 catctttttt gtgagaatca atttgtgcca taatatcggg tgtcaccata tttggaaaca
     7021 tcgaaatctg agcttcgaaa ctttgagtca ttgcttttcc aacggctggc gcatcttgtt
     7081 gttgcaaagc tttccaacaa gcatctgtgg cttcacttaa ccaacgagct cctttttcgt
     7141 ctatttgagt atttgctaaa acatcataat ccatttcacg aggataaagc ggaatcatcc
     7201 aaatgcgttg ttcaatccaa tccagcaatt ccgaaaccat aaccgactgt atatcagatg
     7261 gccaatattc accgtcgtag taatatttat ttaaacctgg cattgttata ccaagtgcat
     7321 cttgagaacc actcacatat tttgttccgg gaggattttc aaaacagaat aaggttttag
     7381 ccaatttttc tttttctcct tcaggaatat cagtatgcca aagttcaatg gctttttttc
     7441 tactgctggt ggacattcca ctgcggtcat taaattcgta atcgggctct acacaaatgg
     7501 taatcactga accacttaca tatttgctaa cataaggctg atcgagccaa ccaccagcaa
     7561 tatcaatacg atatggaatt ctacattcct gacgcaaggc agttgtggaa cgagtaggta
     7621 aattgccgtg agggattcgt ttgctcacga tgtattcgat attcatttct ttgcacaagg
     7681 cttctttttg aggcgtatga ccgtcggtgt ttacaaagaa aatatcaggt tttaaagtgt
     7741 gtagttcttc cagaaaatcg agcaatccgc taccacggtt gatccaagca tcttttacca
     7801 ctttcaaaga attgaccata tacaagcgtt ccgcatctga attgatggtt ttacgtgctt
     7861 tcaattcgtt gattgttttg tctgaaccaa tgccaacata taaatcacct tgttgcgctg
     7921 cttcttcaaa aaatgctatg tgtccgctat gtagcatgtc ataacaacca cttacaaata
     7981 cttttttatt catttttatg ctttatttct attagattta aattctggaa acacaccaaa
     8041 ttttgcatta gtgatacaaa acgacctaca acaattgtaa tgtgtgcaca cacgccagaa
     8101 tttttaaaaa ccaaaagtac aaatttagtg tttagaattt gagttatgag aagttatttt
     8161 tcttaaagga aaaagggttt tggaagatgg gttttttgat agcttcatag agcttctaga
     8221 catttgggaa cctgtttttt tcagggaaga cttcgcaaaa gtgtgattgc ctgttggaat
     8281 gggtactgag taggaaaact tagcacagcg tctagggttt atgaaatact tcgaggagcg
     8341 ggttgcagtt atagggcgtt tttttattta tttctaaaat caatattaat tctatcattg
     8401 gttttccccc aataactaaa cactaccttt cctttaattt cgtcttcttt caaaacacca
     8461 atatatcttg aatccattgc gttatcacga ttgtcaccca tcaaaaaata gctgttttta
     8521 gggattatta tttcgtgttc tgcattttcg tcatcttcaa aaggtttttt aaatgtgtaa
     8581 attttatgct tatggccatt tggtagttct tcttcgtatt ctttaacatc aaattcttca
     8641 cttttagttt ctttaataaa tgaggtttta cattttttat cgttaataat taaaaaattg
     8701 ttctgaattt caagtctatc atttggtaat ccaactattc tataaatcca aggatgtaag
     8761 ctatcttttt tttgaaaaat cacaatgtct ccataatttg gtttaatatt atcgaaagca
     8821 tttaaatctg cgataattct gtctcctact tttatagctg gttcatttga agttgttggg
     8881 attttaaaac ttttaactcc tacaattgaa ttagtatcat aaaaccaaag tattgtataa
     8941 attccaacta taattccaag aaaataatac caagtgttat aagatttagg cttaaaattt
     9001 tttaattttt ttgcatttgt agttgcatca taaataagat aaattctata taaaaaacca
     9061 attactaaaa tagaaatgaa tccagcaaaa aacactccta attttgttaa gccataaaaa
     9121 acaggtaaaa gaagttctat ggttagaaat ataattcctt ttttaatttg accattatat
     9181 atttgaccaa atcctggtcc aaatagtgct aaaagaaaag ctaattttgg atttctaggt
     9241 ttgatttttt cgctattgat ttcagaattc ataatgcttg ttttctcaat atttttccct
     9301 caaatatact caatccattc ctacaaaaaa taaaaaaacc acccaaaatt aagtcttggg
     9361 tggtttgtta aatctgtgag gtaaactatt tagaactatt ttctcacgaa ccaattttca
     9421 atttcaattc catcaatgtt gacaaaatct ttttcattgt ccgttactaa agtcagtttg
     9481 ttttcgatgg ctgttactcc aatcaaaagg tcaaattcgt cgtgcattgg ttttccaatt
     9541 ttgcgaagtc ttactttctc ttttgcatat ttttttattg aactataaat tggaattact
     9601 gtaattccgt ttataaataa atcaactgct ttatgtgatt tagttggatt ctcactgttt
     9661 tcggcaccat aacgaagttc aaaaacagtt atttctgaca caaagcaatt ttctgggttt
     9721 ttatctctga ttatctcgtc taaattcaac tttccccgaa ggaaaaaaac gcaaatattt
     9781 gtgtcaagta aatattgcat tagaatttaa tttctttgtt tctgaatttt ctgcttaatt
     9841 ttaagtctgc aataatttct tctgctgatt tttcatcaga aaaggctcca aacgcctcaa
     9901 aaaaagtatc tttttttgcc gttttttctc tttttagcga cttcgtgagt ttttcaatta
     9961 attctatttt actcaatgaa ctcaatccat caaatagcat tgaataggtt tctacaatat
    10021 gtttgtcagc ataactcatt ttattttttt tgtaaattta aggaatattt ttcatttacc
    10081 gttttttgta aaaatctata tttcagtagc tctggcgtct cttctatctc gaaaccagta
    10141 ttttgttctt cttccctcaa atatactcaa tccattccta taaaaaataa aaaaaccacc
    10201 caaaattaag tcttgggtgg ttgtttagaa ttaccttttt ttatttatta cattttgtgt
    10261 ttaggattga aaccgtctac taattttcgg tgtttgtatt cggcggtcat caattcggca
    10321 taatctactt tttggtcttt gcctaaacgc actcggatac tgtcgaaaat agcatacact
    10381 acaggcacaa caatcaaggt taggaaaagc gaagacatca aacctccgat aactacccaa
    10441 gccaatccgt tgttcatttc agcagcggct cctttggcca aggcgatagg aatcatacca
    10501 attaccatcg caattgtagt catcaaaata ggacgaagtc gagcgtgatt ggcttgaact
    10561 agcgcatcga atgtactttc tcctgcatct ttcctgtggt tggcaaagtc aaccagcaaa
    10621 atcgcattct tacataccaa accaattaac ataatgatac ccaaaatggt aaaaatattc
    10681 aacgagttgt tcgtcaatgc caaggctaac aaggctccaa tgaaagataa tggaatagaa
    10741 aacaatacca caaatggagt tacaaagtca tcatataaag caaccattac aaggtaaacc
    10801 aaaataatcg ctgccaatag ggcaattccc aatgtaccaa aaccttcggt ttggttttcc
    10861 atatcaccac cccaaacata gtttacacct gctggtcttt ccattttaga gaatacagtt
    10921 tcccattcgg tagctactgt tcctgaaggg cgacccacag tttgtgcttc tatcgatacg
    10981 gcagtacttt tgtctctacg ttctagcata cttggaccag aactttcgat aacatcggca
    11041 aattgagata acttgatttg ttgtccggta ctatttagga aaattaaatc gtttacatct
    11101 ttggcattcg aacgatcgta ttcattaaaa cgaatgttga tgtcatactc gtattctcct
    11161 gcacggaatt ttccatcagt atttccgctg aaagccgttt gcatagtcat tcccacagtt
    11221 tgtagtgtca atcctaaagc ggccatttta tcacggttta ccacgacttt aatctctgga
    11281 tttcctgctt ctgaagttaa tttaacctca gatgatcctg gtattttttt caattcggca
    11341 gctgctttgg ttgcaaaagc cattgcgtct tcaaaattcg gaccagtcac agtcagtttg
    11401 ataggggctt ggtcggctcc taataatccc ataggaacgg ttttaacttt ggctccaact
    11461 aatacttttt ctaattcgcg tttgattttt gcagcaaata taaaggagtt gtcttcgcgt
    11521 tcttttttct ccaccaatga aaccaaaatt tctgctttgt aagaggtagc ttgtgtagct
    11581 cccattcctt cgctagtttg tccaacggtg gtaatcaaat cgacaacatt tttatcactt
    11641 cgtaaaaatt cttcggcttt ttgggtcata aaattggttt gctctaccga agcatctttt
    11701 ggcatttcaa tttgcaccaa gaattcacct ttgtcggttt tggcaaagaa ctctccacca
    11761 ataaatccac cgccaactaa tcccattgtc gaggccaaga atagtattga aacaatggcc
    11821 aaggtggttt ttttataaac taaagaccat cttaaaatat cggttacttt gtgagtgaag
    11881 acatctaaat acgattcgaa acctaaaatt actctaccaa aaaatgtctt tttggtgatg
    11941 tgctctaatt ttccataacg agagaacaac caaggcacaa tggtaaatga agccaataat
    12001 gagaataagg tagctataat tactgttaca caaaattgtg taataatatt agaaaccagt
    12061 cccgtactca tcgcaatggg aaggaataca acaatgatta ccaatgtaat tgccattacg
    12121 gtaaaaccaa tttcggcagc tccgtcatag gcagcacgta ctttgttttt acccatttcc
    12181 atatgtcggt gaatgttttc tagaaccaca atcgcatcat ccaccaagat tcctaccact
    12241 aatgaaagtc ctaataaact catcaggtta agcgtgtaac ccattaaata gattccgata
    12301 aaagttgcaa ttaacgaagc cggaatcgaa accattacga taagcgcatt acgaatactg
    12361 tgcaagaaga acagcattac aaaagctacc aagataatgg caataaataa atcgtgcatt
    12421 accgaatcgg cagctgttag ggtaaattcg ctactgtcgt tggctacagc cagtttcaag
    12481 ttcgatttag tataatcttt ctcgatttga acaattgctt tttttacttt tgcgcttact
    12541 tcaaccgcat tggcatccga ttgtttgata acttgaagga tgatggagct tttttgattt
    12601 actctcgaaa ttttctcgac atctttttgg gcatcttgaa catcggcaat gtcgcccaaa
    12661 cgaatttgga cacctttgtt ggaagaaata accaaattac gcatttcttc aaccgatttg
    12721 tattttccgg ataaacgaat ttgtgttttg ttttctctgg ttttgatgtt tcctgttggg
    12781 aaatcaagat tagaagacaa aaccgcttgc tgtacttgtg gaatcgaaag tccgtatcct
    12841 tgcagttttt tagaatctaa acttacttta atttctcttt cttgtccacc tactaagttt
    12901 acttgagcca caccaggaac acgtgacaat attggctgaa tttttttgtc caataaatcg
    12961 tagaacgcaa tttcgtccat ttttccggtg gcaccaatgg tcataatcgg caagtcactt
    13021 agcgaaaatt tgctcaaaga aggcggatcg acatcatcgg gcaggtcttt taataccgca
    13081 ttgatttttc gttgcgcatc attcaaggca taatcggcat cggcttcggt agttaaggtg
    13141 atcattacca ccgaaagact ttcataggat ttggattcga ttttcttgat gttttccatt
    13201 gtggaaatag catcttcaat ttttttggtt acggtgtttt caacctctcc cggcgaagct
    13261 cctggataca cggttgaaac cgtaactacg tttacttcaa attttggaat taattcgtag
    13321 ctcagttgtt ggtaactgaa caaaccaccc aatagtaata tggtaaataa tacaatgact
    13381 attgatgggc gttttatgga tatatcgact attttcattt gttgtcttta aggaaattat
    13441 ttaacgatat ttactttggt gttgtcactc aagttgattt gtccgctagt aacgactacc
    13501 tcgccatcgt tcaatccgtc caggatttct actctgtcgc caaagattct tccagccacg
    13561 atttttttca aagttgccat attgttgttt attacaaata cttggttact actaacacta
    13621 cctacgaaag ctgttcttgg tattgattta acattggttt gttttgcggc ctgggattca
    13681 aaagaggcag ttccatacat tcctgctttg atttcgttgt taggattgtt ggctacttca
    13741 atttcaattg ggaaattcaa actttcatct gcttttgaag caatgaatgt aatttttcct
    13801 gtaaatgatt tgtcgggaaa tacacttgca gaaatactta cattgtttcc taattttaag
    13861 ccagctacct gactttcgtt aactgttact tttaatttta atttagaaac attcacgatg
    13921 tcaaaaagtg ctgttgcagg cattccagtt aaaattgatc ctggttctac atatttttta
    13981 ttgatgtaac ctttaatcgg cgcttttact ttggtgtcgc ccacgttgat gttggcttgt
    14041 ttccaattcg attcggcatt ggtcaaggcc aatctggctt ggtctaattg ttgtttggta
    14101 acaccgcctg ttttgtatgc gttttcatat cgggcataat cggctttaga gttgtcatag
    14161 gctgctttgg ccgcttgtgc gctgatgttg atgacgtcac ctcttacgat tagcaagggt
    14221 tgtcccacat tgacataatc accttctttt gctaaaactt taattacttt tccagaatgt
    14281 tcagccgaaa aggttaattc ttgaatgggt tcaaaatttc cgttggcttt gaacgcatcg
    14341 tctaattttc cggtggttac cgtggcaact cttaccgcaa ccgaactgtt tttttgagct
    14401 acaatggctg tgtcttctgc gtttttcttc ttgttgttga ttaatgtgaa tgctgctcca
    14461 cctattaata cgacagcaat tatgatgtaa attggttttt tcattttgag atatttagtt
    14521 taataagttt tttaattcgc cttttgattt gattaatgct atttcggcaa ttttgtatgc
    14581 tagaatagct cttgtgtaat tgttttgtgc ttcaagcgat gcgttttcag aatccaataa
    14641 atcagttaat gaagctagtc cttgaaggta attgtttttg gtgtttttta aaatatctcc
    14701 agccaagcgc atattttctt tttgattttt gatagttacc aagttgtttt caatttgtgc
    14761 tttagcgttt ctaaaatcca aatcaagtgc aagttgggta tctttaatgt caacttggag
    14821 tgatttaatg gcattgtcag cttgtctcac tctagaacga gttccaaatc ctgtaaaaat
    14881 gggcactttc aaattcaatc caatggatga ataatcggac caatacacgc catcggctgg
    14941 ttttgcaaaa agaggcattt ctggtccttg acctaaataa ttatagcctg caactaacga
    15001 aagtgttggg taatattcgg ctttgatcga tgttttttga aactccaata attcttcttg
    15061 ttttttcaag agcaaatatt cggttctttt ttctgtgctt ggcgcttcgg tcaatgctat
    15121 tggtgttact tcaaattcgg ttttgggcat actgatttga gtttctatag gcatccccat
    15181 ataaaacttc aaggagtttt cttgcaattc gatttgattt ttgatttgct gacggtcagt
    15241 gtcaatgtta gacatcttta caataattcg gtctaaatct attttctttg ccagaccatt
    15301 gtcaaattgt ccttgtacga tgtcacgaac tttggtggtg ttgacataat tgctatctaa
    15361 tagcatcaat ctttcgcgtt gtacgtaaac agagtaataa ttatttgcta ctctttctat
    15421 tacttgctct tcggttaatt gattgtttat ttgatagaat tctcgtgtag aatttgcggc
    15481 tctcaatcct gtgaaaaccg attggtcaaa aatggcttga cttaatgaaa ctcctgctcc
    15541 agaagtccat ttttgtccaa aagacacttg tactgattga cctggttgtc caaacatggc
    15601 tccgtcaaga acgttagttt gcaaaatagg attgtaagtt aaacttccgt tggccgatat
    15661 ttgcggcaag gctcttgagc gcacttcttg aattttatat tcgctgtttt ccatgtcgag
    15721 tctcgatttt tgagcatcgg ctttgttttt tagcgcatag gttatggctt ctttcaaagt
    15781 caatgtttgc acttgtgcac ttgcagttaa gcccaaaatg agtaagggta gtataaatag
    15841 tttcttcatt atagatggta gtttattaat tgtttttcta gttctattat tccttcggga
    15901 gttgccattg ctcgggtatg gtattctaag gccaacaatt ccttttcatt tgcttctttt
    15961 tcggagctta tctttccatt aatactaaaa attagcgtgt aataaaattc aacataagta
    16021 tcaattggtg tttcttttcg atacaaacct tgttcaattc cttttacaat gttttgttta
    16081 aaaactgcat tgcattcatt taattctcga
//
LOCUS       DAOSNN010000026        15721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3751_length_15721_cov_4.817439, whole genome shotgun sequence.
ACCESSION   DAOSNN010000026 DAOSNN010000000
VERSION     DAOSNN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15721
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3751_length_15721_cov_4.817439"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..440
                     /locus_tag="PLQ70_02545"
     CDS             3..440
                     /locus_tag="PLQ70_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQE33520.1"
                     /translation="MAILFLLLKLFEYKLLIIDHSFEIYVGSIALLFTLLGIWLSRKL
                     FSPKKITTIIEKEKIVEKEVFIASPHHFVLNQKAIEETDLSARELEVLSLIAKGLSNQ
                     EIASSLFVSVNTIKTHVTNLFYKLEVTRRTQAVEKAKKLSIIP"
     gene            451..582
                     /locus_tag="PLQ70_02550"
     CDS             451..582
                     /locus_tag="PLQ70_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33521.1"
                     /translation="MAGKTALALGNKEEALKYFTDIKDNYESTPEGATIDVLIGLAQ"
     gene            668..1237
                     /gene="ribH"
                     /locus_tag="PLQ70_02555"
     CDS             668..1237
                     /gene="ribH"
                     /locus_tag="PLQ70_02555"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQE33522.1"
                     /translation="MATINKNLSEYDKNAIPDAKNFRFGIVVSEWNDTITEGLCFGAV
                     TGLLENEVPSEHIIRWNVPGSFELVYGAKKMLQTQNVDAVIAIGCVIQGETKHFDFVC
                     EAVSQGIKDLNVETDIPVIFCVLTDNTMQQSIDRSGGKHGNKGTEAAIAAIKMAYLRH
                     QASFNHQIKNQQLLSSGPLQIEGLKMIEE"
     gene            1399..3255
                     /gene="mutL"
                     /locus_tag="PLQ70_02560"
     CDS             1399..3255
                     /gene="mutL"
                     /locus_tag="PLQ70_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQE33523.1"
                     /translation="MSSIIQLLPDHVANQIAAGEVVQRPASVVKELLENAVDAGANDI
                     KLIIKDAGKSLVQVIDNGKGMSVTDARLCFERHATSKIRQAEDLFSLHTKGFRGEALA
                     SIAAIAHVEMKTKQDQEELGTHIVIEGSKFVSQDVAVLPKGTSFAVKNLFFNIPARRN
                     FLKSDIVEYRHIIDEFQRVALAHPKIYFTFFHNGSEMFNLPASSLRQRIVAVFSGKTN
                     EKLVPVNEETEIVSIQGFVCKPEFAKKSRGEQFFFVNDRFIKSGYLHHAVMAAYEGLL
                     KDGVQPSYFIYLQVPANTIDINIHPTKTEIKFDDESALYAILRASIKHSLGQFNVAPV
                     LDFDRDANLDTPYHYKDLEGATPTIQVDGNFNPFSEEEKPNKHYANSNSSYKRTESVS
                     NWESLYVGLKQDFEENDTLTFENDTVTSSLFNADEVEQVVHKTYQIHKKYIVNPIKSG
                     MIIVDQQRAHQRILYEQFLTNITVQLASSQQLLFPLHLYFSKDEMQLLSELMPSLTNT
                     GFVFEETNDESVVISGLPVNISESEVTIVFEQLLSDLQNGIPESSFSQNDTIAKSMAR
                     SLAVKTGAYLTEKEQENLVNGLFACKDPNVSPFQKPTFITMRVEDLDKKFAL"
     gene            3252..4034
                     /locus_tag="PLQ70_02565"
     CDS             3252..4034
                     /locus_tag="PLQ70_02565"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQE33524.1"
                     /translation="MMNITPVVKQLLIINILFFIGTFVLGGFSMESFNPYNPAFRILS
                     LYFPENPDFHIWQLITHMFMHGGLMHIAFNMFALYSFGSTLEHFWGGKKFLFFYISCG
                     LGAALLHSGINFYTYQIGLNHLTDLGLENQIQNFLKTGGMSFSVRPPDLDQQILHDMF
                     FAYNMPVVGASGAIYGLLAAFAFMFPNAELALMFIPVPIKAKYFVPGILAIDLFLGFK
                     GQSIFGAGSTGIAHFAHVGGALTGFLMMWYWKKNQFNNNRWN"
     gene            4101..4958
                     /locus_tag="PLQ70_02570"
     CDS             4101..4958
                     /locus_tag="PLQ70_02570"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006267028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQE33525.1"
                     /translation="MSSIIDDLKQQYKFGGITQRLIYWNIACFIVSYVIFGLLQIDFL
                     SFVSLSSNPADLLWKPWSILSYSFFHSDFIHIFFNLIVLHFSGQLFLTFFTQKQLLGL
                     YLLSALFSGAVFVVAFYLMEVVSPIVGASGAIMAILVATTVYQPLMNLRLMLIGNVKL
                     WHVTVVVLLIDVMQLRLENSGGHIAHLAGAFFGFVFIKFLQNGTDLSKIVSNLFDFFV
                     NLFTKTESKPFKKVHRNYQKPVSKPASRIVSKDKTQQQIDEILDKISKSGYDCLTKEE
                     KEFLFKAGK"
     gene            5033..6082
                     /locus_tag="PLQ70_02575"
     CDS             5033..6082
                     /locus_tag="PLQ70_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQE33526.1"
                     /translation="MKNLSWFNKIMFFLNIVLTVSTIVAYVLPFLAPKLFPLLSVFTL
                     FMPLFFVLNGLFFLYWGFQFKKRMILSGLVLLMGITFFNKFYKFSAHEYPEDENDFTV
                     MSYNVRLLNVFKWLDRDDVPQDILTFINEKNPDILCIQEFSNSANIDLKVYSHKYVLM
                     EGNQIKTAQAIFSKFPIINQGNIAFPNSNNNVVFVDIKRGKDIIRVYNMHLQSIKISP
                     DVSELDEKNIDAINQGKSQKLFNRISKAFKQQQQQAELIKTHKKDCPYPIIICGDMNN
                     SAFSYVYRNIKGALKDSFEEAGVGFGATYKFRYYPARIDYIFADDKMKVKKFESFSDF
                     ENSDHYPIMAKLSME"
     gene            complement(6092..6715)
                     /locus_tag="PLQ70_02580"
     CDS             complement(6092..6715)
                     /locus_tag="PLQ70_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HQE33527.1"
                     /translation="MDTLLHPSYFPSISHFVAMAQSDKMVFEMEDNFQKQTNRNRMYI
                     YSPNGIQLLNIPVKHSKQAHQKTKEIKIENDFNWQKQHFKSLEAAYRTSPFFEFFEDE
                     ILPIFEKKHHFLMDLNFETMEFAAKCLRMKLDYETTTEYFHEIDSSSILDFRFLVDGK
                     KDASQFESYTQVFGDKHGFLNNLSILDVLFNEGKYALDYLKNQTLIK"
     gene            complement(6781..8484)
                     /gene="lepB"
                     /locus_tag="PLQ70_02585"
     CDS             complement(6781..8484)
                     /gene="lepB"
                     /locus_tag="PLQ70_02585"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQE33528.1"
                     /translation="MTLYQWFVFFLIVQIIHFLGTWKLYEAAGRKRWEAAIPIYNAIV
                     LMKIIGRPTWWTVLLFIPIINLIMFPVIWVETLRSFGKRSSLDTFLGIVTFGFYIYYI
                     NYTQQLNYISDRSLNPENKAADTVSSLLFAIIVATMVHTYLIQPFTIPTSSLEKSLLI
                     GDFLFVSKMNYGARVPMTTVALPMVHDSIPLTKNKSYLSWPQLPYFRLPSFQKIAKND
                     IVVFNWPADTVYQFFDRSGRKAVLKPIDKKSNYVKRCQGTPGDVLEIKDGQVYINGKP
                     LVLPERAKPQYEHTVYSAKGVSNEVLMTTGSTEFNRVYIIKPNSEEQINAVQPYVLSA
                     NQNKDKSFNVITGFKGIPLKVIESSGIYAQEVYDAKADVNLTLKAAAELRKNTTIDSV
                     IRHIAKKTTAFDTSIFPHNTNWTIDNFGPITIPEEGKTVELNQQNLPLYKRIIVNYEG
                     NSLENNGSTIKINGEVTKSYTFKQNYYWMMGDNRHRSEDSRYWGFVPEDHIVGKPVFI
                     WMSWDTNGSGLGKIRWERLFTTVGGDGQPQSYFQFFLIALVAFFVGEYFWKKRKDKKN
                     I"
     gene            complement(8566..9267)
                     /gene="dapB"
                     /locus_tag="PLQ70_02590"
     CDS             complement(8566..9267)
                     /gene="dapB"
                     /locus_tag="PLQ70_02590"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HQE33529.1"
                     /translation="MKIALLGYGKMGQTIERIALERGHEIVLKKDEFNTYEGLSDADV
                     AIDFSIPAVAVENISSCFYTNVPVISGTTGWLDRYDEMVALCEEKKGAFISSSNFSLG
                     VNLFFGLNEYLAKMMSKFDSYKVDMEEIHHTQKLDAPSGTAISLAKGVIENSNYTNWT
                     MENPNSNEIHIEALRIGDVPGTHTVTYNSGIDSIEIKHTAHNREGFALGAVIAAEWIV
                     GKKGVFSMKDVLEMN"
     gene            complement(9279..9800)
                     /locus_tag="PLQ70_02595"
     CDS             complement(9279..9800)
                     /locus_tag="PLQ70_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HQE33530.1"
                     /translation="MGNTSVFSQKKTDATLVANDTLKSNDIDPLTPAKAAFYSAIVPG
                     LGQAYNKKYWKIPIVYGAIGVSMYYYIDSSQKYHQYRDAYKRRLEGYTDDEFSYLDND
                     RLISAQKFYQRNKDFSALFVVGFYVLNIIDANVDAALKQFNINENLSVKPDVHLNDVT
                     FKANVGLTLNYNF"
     gene            complement(9839..10738)
                     /locus_tag="PLQ70_02600"
     CDS             complement(9839..10738)
                     /locus_tag="PLQ70_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQE33531.1"
                     /translation="MAQALKKQALGRGLSALLKDPENDIKSVNDKNADKVVGNIIELE
                     IESIEINPFQPRSNFNEDSLRELAISIKELGVIQPITVRKLDFNKYQLISGERRLRAS
                     TLVGLTTVPAYIRIANDNESLIMALVENIQRHDLDPIEIALSYQRLMDEIQLTQEQMS
                     ERVGKKRSTIANYLRLLKLDPIIQTGIRDGFITMGHGRAIINIENQDVQTDIYQKIVS
                     QNLSVRDTEALVKNYQESLKPKPEGKPKSASFELADAQKNKFNTYFGTKVEVKIAGNG
                     KGKITIPFHSEEDFNRILKLIKD"
     gene            complement(10744..11511)
                     /locus_tag="PLQ70_02605"
     CDS             complement(10744..11511)
                     /locus_tag="PLQ70_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQE33532.1"
                     /translation="MGKIIAIANQKGGVGKTTTSVNLAASLGVLEKKVLLIDADPQAN
                     ASSGLGIDVNNVEIGTYQIIEHSHTPKEALMACTAPNVDVIPSHIDLVAIEIELVDKA
                     EREYMLKKALESIKEEYDYIIIDCAPSLGLLTLNALTASDSVIIPIQCEYFALEGLGK
                     LLNTIKSIQKIHNPNLDIEGLLLTMFDSRLRLSNQVVEEVQKHFNDMVFETIIQRNVK
                     LSEAPSFGESIINYDATSKGANNYLQLAHEIIKKNSK"
     gene            11763..12881
                     /locus_tag="PLQ70_02610"
     CDS             11763..12881
                     /locus_tag="PLQ70_02610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE33533.1"
                     /translation="MTFAIITHVSHIVEQNQYFAYAPYVREMNIWTKYVDELIIVAPK
                     LESKKTPIDIEYEHKNIDFVAIKSFDVLSFKAILGAVLKLPKISWQLYSAMQKADHIH
                     LRCPGNIGLLGCFIQILFPNKTKTAKYAGNWDPKSRQPWTYRFQKWILNNTFLTRNMQ
                     VLVYGEWNEISRNIKPFFTATYYEADKVEIMQRDFQSQFIFVFIGALVSGKNPLYAIQ
                     LVESLYKKGFDVALSLYGEGLERKSLEEYICQNDLSTIVSLKGNQTQETVKIAYESSH
                     FVLLPSESEGWPKAVAEGMFWGCVPVATKVSCVPYMLDYGNRGVLLEMILDEDVHQLE
                     MLLQNENEFNLKSQKAINWSRKYTLDVFEDEIKKMLTS"
     gene            12878..13957
                     /locus_tag="PLQ70_02615"
     CDS             12878..13957
                     /locus_tag="PLQ70_02615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE33534.1"
                     /translation="MRILQIIDSLEAGGAERMAVNYANALAKEIEFSGLVATRKEGAL
                     LNQLEDTVSYLFLNRKGAFDLTALSKLRKFVVENTIEVVHAHSTSFFLAFLLKITYPK
                     IKLIWHDHYGDSEYLSSRPSFSLKLTLPFFIGIIAVNQQLKLWAEQKMHFKNVIYLPN
                     FPAEDDKILEQTILLGIPEKRIVSLANLRAQKDHLLLIEVAQKIKNSHPEWTFHLVGK
                     DFDDDYSKKIKELIIEFNLENTVIVYGSKPDIKNILEQSTIGILTSKSEGLPVALLEY
                     GLCQKPVVVTQVGEIPLLIRDGVNGFLVAQGEIQLFYNSLVKLIEDNNIREDFGKALQ
                     DTIATKYSKNTVLQQYLNWIETRIK"
     gene            13954..15306
                     /locus_tag="PLQ70_02620"
     CDS             13954..15306
                     /locus_tag="PLQ70_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQE33535.1"
                     /translation="MKKDEALYLNIILFHVGIGFLVFLFPFVSKIYGYAIFILGPLYV
                     IKKQNKNNEALMAAAYVVGSEVFLRMTGGNPLYEISKYGVMLFIFIGMYFSGFSKGAT
                     PYWIFLLLLIPSVVLTTFVLNHETNVKNSIAFNISGPVCLAVSSLYTFRRKIPIDQIN
                     TILLFMGLPIVTCMTYLTFYTPNIRDVITSTQSSYETSGGFGPNQVATILGLGMFIFF
                     SRIILESKTKYLLIINLVVALNISYRGMVTFSRGGMITGFLMIILLLLFLYAKSNFRG
                     RVKLNYLIMLVFIAMVTTWGYTSFQTGGLIDKRYANQDASGRKKESKFTGREDVAKGE
                     INVFLDNPFFGVGVGKGVEVRFAETGDGTLSHDEITRMLAEHGTLGIIGLLILFFTPL
                     FLYIENKFNMYLLCFLAFWFLTINHAAMRTAAPAFVYSLSLLNVYFVATPKKSKVVNI
                     "
     gene            15513..>15721
                     /locus_tag="PLQ70_02625"
     CDS             15513..>15721
                     /locus_tag="PLQ70_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQE33536.1"
                     /translation="MKKNKKIHFEVSERKVLLKIFDVVFVLVALYAVGQLLGLQYLID
                     STKYYYYLIAFALYLNSIGTVFEMY"
BASE COUNT         4877 a         2413 c         2850 g         5581 t
ORIGIN      
        1 cactggcaat actttttttg ctgcttaaac tttttgaata caagttgctg attattgatc
       61 acagttttga aatttatgtt ggcagcattg ctttgctctt tactttgttg ggcatctggt
      121 tgtcccgaaa actttttagc cctaaaaaaa taacaacgat catagaaaaa gaaaaaatag
      181 ttgagaaaga agtttttatt gctagccctc atcattttgt acttaaccaa aaagcaattg
      241 aagaaactga tttaagcgca agggaactgg aagtattatc tttaatagca aaagggttga
      301 gcaaccagga aattgcatcc tctctgtttg tttctgtaaa cactataaaa acgcatgtaa
      361 caaatttgtt ttataaactg gaagtaacgc ggcgcacgca ggcagttgaa aaagcaaaaa
      421 aactttctat cattccataa tcatcatact ttggcaggaa aaactgcttt agctttagga
      481 aataaagaag aggcgttgaa atattttacc gatattaaag acaattatga aagtactcca
      541 gaaggtgcaa caatcgatgt attgattgga ttggcacaat aaaatatttt aagaatgcaa
      601 attttagatt ttagattcgt aaattagcaa tttaaaattt aaactttaaa atcttaattt
      661 attaaagatg gcaactataa ataaaaactt atccgaatac gataaaaatg caatcccaga
      721 cgcgaaaaat tttcggtttg ggattgttgt ttcagaatgg aatgatacga taaccgaagg
      781 gctttgtttc ggtgctgtta cgggactttt ggaaaatgaa gtgccatcag agcatatcat
      841 tcgttggaat gttccaggga gttttgaact tgtttacgga gctaaaaaaa tgttgcaaac
      901 tcaaaatgta gatgctgtaa ttgctattgg gtgtgtcatt caaggcgaaa ctaaacattt
      961 tgattttgtt tgcgaagccg tttcacaagg aattaaagac ttgaatgtag aaactgatat
     1021 tccagttatc ttttgtgttt tgacagacaa tacaatgcaa caatccattg acagaagtgg
     1081 aggaaaacac ggaaataaag gaacagaagc agctattgca gctattaaaa tggcatattt
     1141 gagacatcaa gcctctttta atcaccaaat taaaaaccaa caattattat cctcaggacc
     1201 tttgcaaatt gaaggactga agatgattga agaataacac aaattttata attcaaaaaa
     1261 cctatactat tcatttgatg gtataggttt tttgtttttt atgattattt tgggtagtaa
     1321 tcccaataga aaatccttaa atttgctcac ttcggatttt gaattcgaaa tctgcaatct
     1381 aacatctgaa atctaaaaat gtcgagtatt atccaattgc ttcctgatca tgttgctaat
     1441 caaattgctg ctggagaagt cgttcagcga ccggcttcgg tagtcaaaga attgcttgaa
     1501 aatgcagtag atgctggagc gaatgacatc aaattaatca ttaaagatgc cgggaaatca
     1561 ttggttcaag tcattgataa cggaaaagga atgagtgtta ccgatgctcg tttgtgtttt
     1621 gagcgtcatg ctacttccaa aattcgccaa gccgaagatt tgttttcctt gcacaccaaa
     1681 gggtttcgtg gcgaagcatt ggcatcgatt gctgcgattg ctcatgtaga aatgaaaacc
     1741 aagcaagatc aagaagaatt aggaactcat attgttattg aagggagtaa gtttgtatcg
     1801 caagatgtag cggttttacc aaaaggaact tcatttgctg tcaaaaactt gttttttaat
     1861 attcctgctc gccgcaattt cttgaagtca gacatcgtag aataccgcca tatcattgat
     1921 gaatttcagc gagtagcatt ggcacatcct aaaatatatt ttactttttt tcacaacgga
     1981 agcgaaatgt tcaatttgcc agcctcaagt ttgagacaac gaattgtggc tgttttttcg
     2041 ggtaagacga atgagaaatt agttcctgtt aatgaagaaa ctgaaattgt atccattcaa
     2101 gggtttgttt gtaaacctga atttgctaaa aaaagtcgtg gcgagcagtt tttctttgtc
     2161 aatgaccgat tcataaaaag tggttatttg caccacgcag ttatggctgc ttatgaagga
     2221 ctgttgaaag atggtgtaca gcctagttat tttatttacc ttcaagtgcc tgccaatact
     2281 atagatatta atattcatcc caccaaaacg gagatcaagt ttgatgatga atcggctttg
     2341 tatgccatat tgcgggcttc gataaaacat agtttaggac aatttaatgt ggcacctgtt
     2401 ttggatttcg atcgtgatgc caatttagat acaccatatc attataaaga tttagaagga
     2461 gcaactccaa ctattcaggt tgatggcaat ttcaatcctt tttcagaaga ggagaaacct
     2521 aataaacatt atgcaaattc caattctagt tacaaaagaa ctgaatcagt atcaaattgg
     2581 gagagtttgt atgtgggttt gaaacaggat tttgaggaaa atgacacgct aacttttgag
     2641 aatgacacag taacttcctc tttgtttaat gctgatgaag tcgagcaagt agttcataaa
     2701 acctatcaaa ttcataagaa gtatattgta aacccgatta agtcaggaat gataattgtg
     2761 gatcaacagc gagcacacca acgtattttg tatgaacaat ttttgaccaa tattacagtt
     2821 caattggctt caagccagca attattgttt ccgttacatt tgtatttttc gaaagatgaa
     2881 atgcaattat tatctgaatt gatgccgtct ttgaccaata ccggtttcgt ttttgaagag
     2941 acaaacgacg aaagcgttgt aatttctggt ttgcctgtaa acatatcaga aagcgaagtt
     3001 acaatcgttt ttgagcaact tttgagtgat ttacaaaacg gaattccaga gagtagcttt
     3061 agtcagaatg acaccattgc caaatcgatg gctcgaagtt tggccgtaaa aacaggagcc
     3121 tatttaaccg aaaaagaaca agaaaacttg gtcaacggac tttttgcttg caaagatcct
     3181 aatgtttcgc cttttcaaaa acctactttc atcacgatgc gtgtggaaga tttagataaa
     3241 aaatttgcac tatgatgaac atcacaccag ttgttaagca actattgatt attaatattc
     3301 tttttttcat tggaactttt gtattaggag gtttttcaat ggaaagtttt aatccctata
     3361 atccagcttt tagaattctg tccttatatt ttccagagaa tcccgatttt catatttggc
     3421 aattaattac ccatatgttc atgcatggag gattaatgca tatcgctttt aatatgtttg
     3481 ctttatattc ttttggctct actttggaac atttttgggg tggaaaaaaa ttcttgtttt
     3541 tctacatttc ttgcggatta ggggcggcat tgttgcattc aggaattaat ttttatactt
     3601 atcaaattgg tttgaaccat ttgactgatt tgggtttgga aaatcaaatt caaaattttc
     3661 ttaaaactgg cggaatgagt ttttcagtta gacctccaga tttagaccaa caaattttac
     3721 atgacatgtt ttttgcctat aatatgcctg ttgttggagc ttctggagct atttatggat
     3781 tattagccgc ttttgctttt atgtttccta atgctgaatt ggctttgatg tttattccag
     3841 ttccgattaa ggcgaaatat tttgtacctg gaattttagc tattgatttg tttttgggat
     3901 tcaaaggaca atccattttt ggcgcaggaa gtacagggat agctcatttt gcccacgttg
     3961 gtggtgcctt gacaggtttc ttaatgatgt ggtattggaa gaaaaatcag tttaacaaca
     4021 atcgttggaa ttaattttag taatcagtgt tcagaatgca ggcttactaa tgactcataa
     4081 ctcataattc ataactcaat atgagcagta taattgacga tttaaaacag cagtacaaat
     4141 ttggtggaat aactcaaagg ttaatctact ggaatattgc ttgtttcata gtctcgtatg
     4201 tgatttttgg attattgcaa atcgatttcc tttcgtttgt tagtttgtct tcaaatccag
     4261 ccgatttgtt gtggaaacct tggtcaatac ttagctattc tttttttcat tccgatttca
     4321 ttcacatttt tttcaatctg atagtgctgc atttttcggg ccaattgttt ttgacttttt
     4381 tcactcagaa acagttattg ggattgtacc ttttatcggc tctattttcg ggtgctgttt
     4441 ttgttgttgc attctattta atggaggttg tttcacctat tgttggggct tctggtgcca
     4501 taatggccat tttggttgca actaccgttt atcaaccttt gatgaactta cgcttgatgc
     4561 ttattggcaa tgtcaagctt tggcatgtta cagttgttgt acttctaatt gatgtgatgc
     4621 aactccgttt agaaaattca ggcggacata ttgcgcattt ggctggtgct ttttttgggt
     4681 ttgtatttat taaattcctt caaaacggaa ccgatttgag taaaattgtt tcgaacctat
     4741 tcgatttttt tgttaatttg tttacgaaaa ccgaatctaa gcctttcaaa aaagtgcata
     4801 gaaattatca aaaaccagtt tctaaacccg cttctaggat tgtatccaaa gataaaacgc
     4861 aacagcaaat agatgagatt ttggataaaa tcagtaaatc gggatacgat tgtttgacaa
     4921 aagaagaaaa agaatttttg tttaaagcag ggaaataagc gttcagtaat tggtgttcag
     4981 taatcagtgt ttagtgttca gtcgcagttt gcagtaatga aattaatatg aaatgaaaaa
     5041 cctttcgtgg tttaataaaa taatgttttt tttgaatata gtgctaactg tatccactat
     5101 agttgcttat gttttgcctt ttttagcgcc aaaacttttt ccgcttttgt ctgtttttac
     5161 cttgtttatg ccgttgtttt ttgttttgaa tggcttgttt ttcttgtatt gggggtttca
     5221 gttcaagaag cgaatgattc tgtcgggttt ggttcttttg atgggaatca ctttttttaa
     5281 taaattctat aagttttcag ctcacgaata tcccgaagat gagaatgatt ttacggtaat
     5341 gagttataat gttcgattgt taaatgtttt taaatggctg gatagagatg atgtaccaca
     5401 agatattttg acatttatca acgaaaaaaa tcctgatatt ttgtgcattc aggagttttc
     5461 aaattctgcg aatatcgatt tgaaagtata tagtcataaa tatgttttga tggaaggaaa
     5521 tcaaatcaaa acggctcagg ctattttttc taaatttcct attataaatc aaggaaatat
     5581 tgcgtttccc aattcgaata ataatgttgt ttttgttgac atcaaaagag gaaaagacat
     5641 tattagggtt tataatatgc atcttcaatc cataaaaatt tctcctgatg tttctgaatt
     5701 ggacgaaaaa aatattgatg ccatcaatca aggtaaatca caaaaacttt ttaatagaat
     5761 aagcaaagct ttcaagcaac aacagcaaca agcagaactg attaaaaccc acaaaaaaga
     5821 ttgtccctat cctataatca tttgtggcga tatgaataat agtgcttttt cttatgtgta
     5881 tcgaaatatc aaaggtgctt tgaaagatag ttttgaagaa gcaggagtag gttttggagc
     5941 cacctataaa ttcagatatt atcctgccag aatcgattat atttttgcag atgataaaat
     6001 gaaagtcaag aaatttgaaa gtttttctga ttttgaaaac tcagatcatt atcccattat
     6061 ggcaaaattg tcgatggaat aaaagagaaa attatttgat taatgtttga tttttcaaat
     6121 aatccaaagc atattttcct tcgttgaaca atacatctaa aatgctcaaa ttgttcaaaa
     6181 atccgtgttt atcaccaaaa acctgagtgt aactttcgaa ttgtgatgcg tctttttttc
     6241 catcaaccaa aaatctgaaa tctaaaatac tactcgaatc tatttcatga aaatattcgg
     6301 ttgtagtttc ataatccaat ttcattcgca aacacttggc ggcaaattcc atagtttcaa
     6361 aattcaaatc catcaaaaaa tggtgttttt tttcgaaaat gggaaggatc tcatcttcaa
     6421 aaaactcaaa aaaaggcgaa gttctataag cagcttctag tgatttgaaa tgttgttttt
     6481 gccaattgaa atcattttca attttgattt ctttggtttt ttgatgggct tgttttgaat
     6541 gttttacggg aatattcaag agttgtattc catttggact gtaaatatac atccggttgc
     6601 gattggtttg tttttggaaa ttatcctcca tttcaaacac cattttgtca gattgagcca
     6661 tagctacaaa gtgactaatt gaagggaaat aggagggatg taataaagtg tccatctgtt
     6721 tgtttttttg tttaaagttt caggttatgg aacttgaaac tttaaacctg aaacttataa
     6781 ctaaatattt tttttatctt ttcttttttt ccaaaaatac tcgcccacaa aaaaggcaac
     6841 tagggcaatc aagaaaaatt gaaaatacga ttgtggttga ccatcaccac caacggttgt
     6901 gaataagcgt tcccaacgaa ttttgcccaa tcctgaccca tttgtgtccc aactcatcca
     6961 aatgaaaacg ggtttcccta cgatatgatc ttctggaaca aaaccccaat aacggctgtc
     7021 ttcagagcga tggcgattgt ctcccatcat ccaataataa ttttgtttga aagtgtatga
     7081 tttggtaact tcaccattta ttttgattgt gctgccatta ttttcaagag aattaccttc
     7141 ataatttaca ataattcttt tgtaaagagg taggttttgt tggtttaatt ctactgtttt
     7201 tccttcttca ggaattgtta ttgggccaaa attatcaata gtccaattgg tattatgtgg
     7261 aaatatactg gtgtcaaatg cggttgtttt ttttgcaata tgcctaatta cagagtcaat
     7321 tgtagtattc ttacgtaatt cggcagcagc ttttagagta aggttcacat cggcttttgc
     7381 atcataaacc tcttgggcat aaattcctga actttcaatc acttttaacg gaattccttt
     7441 gaatccagtt atcacattaa atgatttgtc tttattttga tttgcactta aaacataagg
     7501 ttgaactgca tttatttgtt cctcgctatt tggttttatg atgtaaactc tattaaactc
     7561 tgttgagcca gttgtcatta atacttcgtt tgaaacgcct tttgcagagt aaacagtatg
     7621 ttcgtattgt ggttttgctc tttcaggaag aaccaaaggt ttaccattta tatatacttg
     7681 accatctttt atttctaaaa catcacctgg tgttccttgg catcttttta cataatttga
     7741 ttttttgtca atgggtttca gaactgcttt tctgcctgat ctatcaaaaa attgatacac
     7801 tgtatcggca ggccagttga aaactacaat gtcgttttta gcaatttttt gaaaagaagg
     7861 taatctaaaa taaggcagtt gtggccaact caaataggat ttgttttttg ttaaaggaat
     7921 agagtcgtga accatcggta aagctactgt ggtcatcgga actcttgctc cataattcat
     7981 tttactaaca aataaaaaat caccaataag taatgatttt tctaatgacg aagttggaat
     8041 tgtaaagggt tgtatcaaat aagtgtgtac cattgtagcc acaatgatgg caaaaagcaa
     8101 agaacttacg gtgtctgcag ccttgttttc tggatttaaa cttcggtctg aaatgtaatt
     8161 taattgttgg gtataattaa tatagtaaat gtagaaaccg aaagttacaa ttcctaaaaa
     8221 ggtatctaat gaagaacgtt ttccaaagct tctcaatgtt tctacccaaa taactggaaa
     8281 cataatcagg ttgataatag gtatgaaaag taagactgtc caccaagttg gacgaccaat
     8341 aattttcatt aaaacgatgg cgttatagat tggaattgca gcttcccagc gttttcttcc
     8401 tgctgcttca tataatttcc aagttcctag aaagtgaatt atttgtacaa ttaggaaaaa
     8461 tacaaaccat tgatatagtg tcataattat tagattttag attttagatt ttagatttcg
     8521 gttgaaattt aaaatctaaa tatttttatt atgtttagaa cttttttagt tcatttctag
     8581 aacatctttc atagagaaaa cccctttttt tcctacaatc cactcggctg caattacagc
     8641 accaagtgca aaaccttcgc gattgtgagc ggtgtgtttt atttctatag aatcgatgcc
     8701 agaattgtaa gttacagtat gtgttccagg aacatcacct attcttaggg cttcaatatg
     8761 aatctcattt gaattaggat tttccattgt ccaattagtg taattgctat tttcgataac
     8821 accttttgcc aatgaaatgg cggttccact aggagcatct agtttttgag tatggtgaat
     8881 ttcttccata tctactttat aggaatcaaa ttttgacatc atttttgcca aatattcatt
     8941 gagtccaaag aaaagattta cgcctaagct aaaattagaa ctcgagataa aagctccttt
     9001 tttctcttcg caaagtgcta ccatctcgtc ataacggtct aaccagcctg ttgttcctga
     9061 aattacaggc acgttggtat aaaaacaact cgatatgttt tctacagcta cggctggaat
     9121 actaaaatcg atagcaacat cggcatctga aagtccttca taggtgttga attcgtcttt
     9181 tttcaaaaca atttcgtgtc ctctttctag tgcaattctt tcgatggttt gacccatttt
     9241 cccgtatcct aaaagtgcaa ttttcatttt ttttatttct aaaaattata atttaatgtt
     9301 agtccaacat tggccttaaa tgttacatca ttcagatgaa catcgggctt aacagacaag
     9361 ttttcattga tattgaattg ttttaaagct gcatcaacat tggcatcgat gatgtttaaa
     9421 acataaaaac ctacaacaaa taatgctgag aaatctttgt ttctttggta gaatttttga
     9481 gcagaaatta atcggtcatt gtctaaatag gaaaactcat catcggtata tccttctaaa
     9541 cgtcgcttgt aggcatctct gtattgatgg tatttttggc tactgtcaat ataataatac
     9601 atactaacgc ctattgctcc ataaacaatt ggaattttcc agtatttctt gttgtatgct
     9661 tgtcctaaac cgggtacaat agcggaataa aaagccgctt ttgctggtgt caaagggtct
     9721 atgtcgttcg atttcaaagt atcatttgca accaaggtag cgtctgtttt cttttgtgaa
     9781 aaaacagagg tgtttcctat agcaaaaaag catagaacta tgaaaaaaaa agtacgcact
     9841 agtcttttat tagtttaagt atcctgttga agtcttcttc tgaatggaat gggatggtta
     9901 tttttccttt accatttcct gctattttta cttctacttt tgttccaaaa taagtgttga
     9961 atttattttt ttgggcatcg gcaagttcaa aagaagctga ttttggtttt ccttcaggtt
    10021 ttggtttcaa actttcttga taatttttta ccaaagcttc agtgtctctg actgatagat
    10081 tttgactaac aattttttgg taaatatcag tttgaacatc ttgattttca atattaatga
    10141 tagcacgtcc gtgccccata gtaatgaaac catctctgat tccagtttgg ataattgggt
    10201 ctaattttaa aagtcgtagg tagttggcaa ttgtcgaacg ttttttacca acacgttcgc
    10261 tcatttgctc ttgtgtgagt tgaatttcgt ccattaatct ttgataagaa agtgcaattt
    10321 ctattgggtc taaatcatgg cgttgaatat tttcgaccaa tgccatgatc aaagattcgt
    10381 tgtcatttgc aattcgaatg taggcaggaa ctgtagtcaa tccaactaaa gtagaagcac
    10441 gaagacgtcg ttcacccgaa attaattggt atttattgaa gtctaatttt cgaacagtaa
    10501 tgggttgaat aactcctaat tccttgatgg aaatcgctaa ttctctcaat gaatcttcgt
    10561 tgaaattgct tctaggctga aacggattga tttcgataga ttcaatttct agctcaataa
    10621 tatttcctac aactttatct gcatttttat cattaaccga ttttatgtcg ttttctgggt
    10681 ctttcaataa tgccgataat cctcttccta acgcttgttt ttttagtgct tgtgccataa
    10741 aaattatttg ctgtttttct ttataatttc gtgagctaat tgtaggtagt tgttggctcc
    10801 tttgctcgtt gcgtcatagt ttataatact ttcgccaaaa cttggtgctt cactcaattt
    10861 tacatttcgt tgaataatag tttcaaaaac catatcgtta aaatgttttt gaacctcttc
    10921 gactacttga ttggacaaac gcaatctaga atcaaacatg gtcaacaaca agccttcgat
    10981 atccaaattt ggattatgaa ttttttgaat gcttttgatg gtattcaata attttcccaa
    11041 accttcgagc gcaaaatatt cgcattgaat aggaataata actgaatcgg atgcggtcaa
    11101 agcattcaaa gtaagcaatc ctaacgatgg agcgcaatca ataatgatat agtcatactc
    11161 ctctttgata ctttccaatg cttttttgag catatattct cgctcagctt tatctactaa
    11221 ttcaatttcg atggcaacaa ggtcaatatg cgatggaatc acatcaacat ttggagctgt
    11281 acacgccatt aaagcctctt ttggcgtatg gctatgttct ataatttgat aggttccaat
    11341 ttctacatta ttaacgtcga ttcccaatcc tgaactcgca tttgcttgag gatcagcatc
    11401 tattaataac actttttttt ctaaaacacc tagtgatgct gccagattta cggatgttgt
    11461 tgtctttcca acgccacctt tttgattagc aatcgcaatg attttgccca tttatttttc
    11521 taaattttga acggtaaaaa tacaattatt tatggtttct aaaaatctat ttttgttaac
    11581 atttgttaag gtttgattag cagtagtatt ttttgcaaaa ggttcttagg gattcaataa
    11641 ttatctgggt ttagtttgaa tgtaattgtc tgccataaaa tttaaattct cgtattgata
    11701 ttgaatagat ttagcaatac atttaccgtc aaaaaattat attttcaacc caatactttt
    11761 taatgacttt tgcaatcata actcatgtgt ctcatattgt tgagcaaaat cagtattttg
    11821 cttatgcccc atatgttcgt gaaatgaata tttggacaaa atatgtagat gagttgatta
    11881 ttgtagctcc aaaattagaa tccaaaaaga caccaatcga catagaatat gaacacaaaa
    11941 acattgattt tgttgcgata aaaagttttg atgtattgag tttcaaagct attttgggcg
    12001 cggttttgaa gttgccaaaa atcagttggc agctttattc cgccatgcaa aaagccgatc
    12061 acattcattt gcgttgtcct ggaaacatag gtttattggg atgttttatt cagattttat
    12121 ttcccaataa aacaaaaact gctaaatatg ctgggaattg ggatccaaaa tcacgacaac
    12181 cttggaccta tcgttttcaa aaatggattt tgaataacac ttttctaact cgtaatatgc
    12241 aggttttggt ttatggagaa tggaatgaaa tttctagaaa tataaaaccc ttttttacag
    12301 cgacctatta tgaagccgat aaagttgaaa tcatgcaaag agattttcaa agccaattta
    12361 tttttgtatt tattggtgct ttggtttcgg gaaaaaatcc gttatatgct attcaactgg
    12421 ttgaaagttt gtataaaaaa gggtttgatg tcgcattatc tttatatgga gaaggtttag
    12481 aaagaaaaag tctcgaagaa tatatttgtc aaaatgattt aagtactatt gtttcattaa
    12541 aaggaaatca aactcaggaa acggttaaaa ttgcctacga atcaagtcat tttgttcttt
    12601 tgccttctga aagtgaaggc tggccaaaag cagttgccga aggtatgttt tggggctgtg
    12661 ttcctgtagc cacaaaagtt tcttgtgttc cgtatatgtt agattatgga aatcgaggtg
    12721 tattattaga aatgatttta gatgaagatg ttcatcaatt ggaaatgctt ttgcaaaacg
    12781 aaaacgaatt taatttgaaa agtcaaaagg cgattaattg gtccagaaaa tatacgttgg
    12841 atgtttttga agatgaaatt aaaaaaatgt tgacatcatg aggatactac aaattataga
    12901 ttctcttgag gctggtggag ctgaacgaat ggcggtgaat tatgccaatg cgcttgccaa
    12961 agaaatcgag ttttcgggat tagtggcaac tcgaaaagaa ggagctcttt tgaatcaatt
    13021 agaagatacg gtttcttatc tttttttgaa tagaaaaggc gcatttgatt taacagctct
    13081 atctaaattg cgaaaatttg tggttgagaa cactattgaa gttgttcacg ctcatagcac
    13141 gtcttttttt cttgcttttc ttttgaagat tacctatcct aaaattaaat tgatttggca
    13201 cgatcattat ggtgatagtg aatatttgtc gagccgacct tcttttagtt taaaattaac
    13261 tcttcctttt tttattggaa ttatagcagt aaatcagcaa ctcaaattat gggccgaaca
    13321 gaaaatgcat tttaaaaatg ttatttattt gcctaatttt cctgctgagg atgataaaat
    13381 tttggaacaa actattttac ttggcattcc agaaaaacga atagtatctt tggccaattt
    13441 aagggcgcaa aaggatcatt tattattgat tgaggtggcc caaaaaatta aaaattcaca
    13501 ccctgaatgg acatttcatt tggtaggtaa agattttgat gatgattatt cgaaaaaaat
    13561 taaggagtta attattgaat ttaatttaga aaataccgta attgtatatg gttctaaacc
    13621 cgatataaag aacatacttg agcagtcaac cattgggata ttaacttcta aatcagaagg
    13681 attgcctgtc gctttgcttg aatatggttt gtgtcagaaa cctgtagtgg taactcaagt
    13741 aggcgaaatt cctttattga ttcgagatgg ggttaatggt tttttggttg cccaaggcga
    13801 gattcaatta ttttacaact cattggttaa attgatagag gacaataaca ttagagaaga
    13861 ttttggtaag gcacttcaag atacaattgc aacaaaatat tctaaaaata ctgttttgca
    13921 gcagtattta aattggattg aaacaagaat aaaatgaaaa aagacgaagc tttatatctt
    13981 aatataattc ttttccatgt tggaattgga tttttagtgt ttctatttcc gtttgtttca
    14041 aaaatatatg ggtatgctat ttttatttta ggccctttgt atgtaataaa aaagcaaaat
    14101 aaaaataatg aagccttaat ggctgctgct tatgtggtag gaagtgaggt tttcttgcga
    14161 atgacaggag gaaatccgtt atacgaaatt tccaaatatg gtgtgatgct atttatattt
    14221 attgggatgt attttagtgg attttcaaaa ggagctacgc cctattggat ttttttattg
    14281 ttactaattc caagtgtggt gttgactact tttgtgttga atcatgaaac taatgtcaaa
    14341 aacagtattg cttttaatat ttcaggtcca gtatgtttag ctgtgtcttc tctttatact
    14401 tttaggcgca agattccaat agatcagatc aatacaattc ttctttttat gggtttgcct
    14461 atcgtgactt gtatgaccta tttaactttt tatacaccca atattcgaga tgtaattacg
    14521 agtacccaat ctagttatga gacctctgga gggtttggtc ccaatcaggt tgctacaata
    14581 ttaggattgg gaatgtttat ctttttttct aggattattc ttgaatctaa aaccaaatat
    14641 ttgctcatca ttaacttggt tgtagctctt aatatcagtt atcgagggat ggtaactttt
    14701 tctagaggag gaatgattac tggttttttg atgattatat tgttattgtt gtttctatat
    14761 gcaaaatcta attttagagg tagagttaaa ctgaattatc taattatgtt ggtattcata
    14821 gctatggtta ctacttgggg atacacttct tttcagacag gaggactaat tgataagcga
    14881 tatgcaaatc aggatgcgtc aggaagaaaa aaggaaagca aatttacagg aagagaggat
    14941 gtggcaaaag gagaaatcaa tgtctttttg gataatccat tttttggagt tggagtagga
    15001 aaaggggtag aggttcgatt tgccgaaaca ggtgatggta ctttgtctca tgacgaaatt
    15061 actagaatgc ttgctgaaca tgggactttg ggaattatag gcttgttaat tctgtttttt
    15121 actcccttgt ttttatatat tgagaataag tttaatatgt atttactctg ttttctggct
    15181 ttctggtttt taacaataaa tcacgcagca atgcgaactg cagcaccagc atttgtttat
    15241 tctttatcat tacttaatgt ttattttgtg gcaaccccta aaaaaagtaa agtggtaaat
    15301 atataatgca ggcttattta attaggattt gcactttact tgttttttct agatgctaaa
    15361 tatttaaaat ttgcccaata aaatatgtaa ttgataaaac atttgctctt tgagtgagtt
    15421 aagatgatta tagttgactt taatcgattt tttattggtt tcattgtagg attatgtaga
    15481 tttgttgtgc aaaaaatata agagaaatag taatgaaaaa gaataagaaa atacattttg
    15541 aagtctccga gcgaaaagta ttgctcaaga tttttgatgt agtttttgtt ttggtggcgc
    15601 tgtatgctgt aggacaattg ttagggttgc aatatctcat agattcaacg aaatactatt
    15661 attacttaat tgcatttgcg ctttatctca atagcatcgg gacggttttt gaaatgtata
    15721 a
//
LOCUS       DAOSNN010000027        15601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3788_length_15601_cov_5.081436, whole genome shotgun sequence.
ACCESSION   DAOSNN010000027 DAOSNN010000000
VERSION     DAOSNN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15601
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3788_length_15601_cov_5.081436"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLQ70_02630"
     CDS             complement(<1..774)
                     /locus_tag="PLQ70_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HQE33537.1"
                     /translation="MKWIGRRQSDNVDDRRGMSGGGKTLIGGGVLGIVILLLNAFGGE
                     NGQMIGNILQQTQGQQGAPTEERALTPEEVKEGEFVKTLLADNEDVWTKIFAENNLKF
                     EAPKLVLFSGQVQTACGGASSASGPFYCPGDRIIYMDMTFFEELRTKFGAEGGDFAVA
                     YVLAHEYGHHIQTLLGTSAKMRQAQEGKSEAEANKLSVALELQADFYAGLWTHYNEKQ
                     NAMLEPGDIEEALSAANAVGDDAIQKKMQGQVVPDSFTHG"
     gene            complement(814..1620)
                     /locus_tag="PLQ70_02635"
     CDS             complement(814..1620)
                     /locus_tag="PLQ70_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33538.1"
                     /translation="MQNTNTQKDQIENLCANGYQLDFGIVFESAFENYKKIALYAGLM
                     ILIFWILISIVFGVSLVAFIGVEHMEEFGNKMKQISENKVMPLDIAIPINMGLLLFSA
                     LINPFMAGFLKMADCGEKGEEFHVSTMFSYYKYPYFYAIFLSTLLAGFISTGAALLLE
                     YAGLSFVGTVISLLVSFLTFLTIPLIVFAKLNAIDAIKSSILLISKQPLVLLGLIIVA
                     GIGVALGIFGLCIGIFFTWPFIYSMNYAIYKNIIGIDETSEIDKITGTEN"
     gene            complement(1651..2979)
                     /locus_tag="PLQ70_02640"
     CDS             complement(1651..2979)
                     /locus_tag="PLQ70_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HQE33539.1"
                     /translation="MESKTNINFKKETLTKLLNAILIHENEIIDALYFDFKKPAFEAV
                     LTETGYVISELKETIKNINKWAKPRRVFPSLLNFPSKDYIYKEPYGKVLIISPWNYPF
                     QLALCPLISAFAAGNEVVLKPSELTPKTSEIITKIISKVFDKKDITVIQGGIEVSQQL
                     LSERWDYIFFTGSVAVGKIVAKAAAENLTPVTLELGGKNPCIIDETANLKLAAKRIVW
                     GKFMNAGQTCIAPDYILIQKNMKSHFVDYMKEEIIKAYGDNPSDSPDFARIINTKNCQ
                     RLINMIDQKKVIFGGQNDIKNNYIAPTLIDENSIDSELMKDEIFGPILPIITYEKEKQ
                     IDAVISKYEKPLAFYLFSENRTFTKHLIQKYSFGGGCVNDTMVHFSNKRLPFGGVGHS
                     GIGAYHGILSFDTFSHKKSILIKSNLLDLPFRYAPYKGKLATIKKILDWI"
     gene            complement(3025..3714)
                     /locus_tag="PLQ70_02645"
     CDS             complement(3025..3714)
                     /locus_tag="PLQ70_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HQE33540.1"
                     /translation="MKILSDKNNLQILHEDNHIIVVNKRVGDIVQGDKTGDKPLSEVV
                     KEYIKDKYNKPGEVFLGVVHRLDRPTTGIVVFARTSKALSRMNELFSNRETQKTYWAV
                     VKNKPENTQDKLVHYLKRNEKNNTSKAHSREVPDSKLASLDYTIIKELNNYFALEINL
                     HTGRHHQIRAQLSAIGCPIKGDLKYGFDRSNPDGGIHLHARKLVFIHPVSKENLSIVA
                     PVPNDVIWKAI"
     gene            complement(3732..5216)
                     /locus_tag="PLQ70_02650"
     CDS             complement(3732..5216)
                     /locus_tag="PLQ70_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQE33541.1"
                     /translation="MISKKLKDTNFILCFSVITILIFRLILTFAIPLLDKTEARYAEI
                     ARLMYETKDWIVVQIDYGFPFWAKPPLSTWLSAGSFEIFGANEFSARFPSYLLSVLLL
                     FIAGKLAKKEGKSFYLPGFILLTMPEFLIHTGVVSTDSALFVCITIMMLSFWKVVNNT
                     EKTIWNYLFFIALGLGFLAKGPLIMVLTFPPLFVWCLLDLNRLKAVFSRLPIILGILI
                     VVAIALPWYYLIEQKSPGFTNYFFVGEHYKRFLVPGWNGDLYGNPKYVPMGIVWVYMI
                     AFAFPWFPIVVYKLWKNRTTILKNPWVSFLVLWLLWLPFFFTFSKNILHTYILPTTFP
                     MMLLMVHYWNEFDSKKTLIRIGLFFPTAIFVIASVAILLPKDLFTKLSGYPDVSYIMN
                     SDKYLLEKLKVNTENKQIPLYYFVKVENDSVVNKQKNYSGEFYSNGKEQVLINQKEVD
                     SVLSLNKKLFFVIPTRKLEQVSKESLEKMNLIDHNNNTSVFESK"
     gene            complement(5220..5990)
                     /locus_tag="PLQ70_02655"
     CDS             complement(5220..5990)
                     /locus_tag="PLQ70_02655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE33542.1"
                     /translation="MTIIPNTTVVIPCYNEEKGISNAEYSNFLNNNPEVLICFVNDGS
                     KDNTLAVLTVLKEKHPTQIQILSLEKNAGKAEAVRAGINHCNLHFQHRYIGYLDADLA
                     TTLEEFIELRNYLQGEIVFSFGSRIRKIGSIIERENSRFLIGRVVATFISNILDIKVY
                     DTQCGSKLFTREISEKVFEKEFISRWLFDVEIFYRMIHLFGKEKAIKKMIEVPLKLWV
                     EKGDSKVKFTYGFKLWFDLFEIQRKYKKIAKNLSNPNA"
     gene            complement(6032..6850)
                     /gene="panB"
                     /locus_tag="PLQ70_02660"
     CDS             complement(6032..6850)
                     /gene="panB"
                     /locus_tag="PLQ70_02660"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQE33543.1"
                     /translation="MSTAKKDYKRITTKSLIEMKADGQKISMLTAYDFTMAKIVDAAG
                     VDVILVGDSASNVMAGHETTLPITLDQMIYHASSVVRAIYRALVVVDLPFGSYQSDPK
                     EALRSSIRIMKESGGHAVKLEGGSEIKESIKRILNAGIPVMGHLGLTPQSIYKFGTYT
                     VRAKEEEEAEKLIEDAKLLEKLGCFAIVIEKVPAELAQKVAKSISIPVIGIGAGGGVD
                     GQVLVIHDMLGMNNEFSPRFLRRYMNLYEGMTSAIGQYVADVKSQDFPNEKEQY"
     gene            complement(6985..7356)
                     /locus_tag="PLQ70_02665"
     CDS             complement(6985..7356)
                     /locus_tag="PLQ70_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HQE33544.1"
                     /translation="MLKNNQLYYFTLTIIVMIMGVISRKIDNVPTFIGDGLYACMIYF
                     GMRILFVNSNTKKAAILALLFCFVIELQQLYRADWILAIRNTTLGHYVLGQGFLWSDL
                     CFYTFGIAIAFAIDLRSLNRN"
     gene            complement(7372..8337)
                     /locus_tag="PLQ70_02670"
     CDS             complement(7372..8337)
                     /locus_tag="PLQ70_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HQE33545.1"
                     /translation="MKNENQTKKSILDKTITTREVIFMFFVVLCLSPLISPPIALLMG
                     LIIAQFIGHPYLNLNHKATHILLQVSVVGLGFGMNVNSALKAGKEGIVLTIASIIGTI
                     LIGIFLGKILKIEEKISYLIATGTAICGGSAIAAISPVIKANEKQISVALGTIFILNS
                     IALVAFPYIGHYLNLSETQFGMWCAIAIHDTSSVVGAAGKYGSQALEVATTVKLARAL
                     WIIPIAFLSTFVFKNKDTKIKIPYFIGLFILVMMANTYLPFVQNFSHYITGFAKATLT
                     LTLFLIGCGLSRKVLVSVGYKPLIEGIVLWIIISFSTLWAVLTWV"
     gene            complement(8443..9870)
                     /locus_tag="PLQ70_02675"
     CDS             complement(8443..9870)
                     /locus_tag="PLQ70_02675"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HQE33546.1"
                     /translation="MEECISVFDMLKIGVGPSSSHTLGPWRAAERFLGELRANTIFET
                     VHHIKVDLFGSLSLTGKGHATDLAIMLGLSGQDPEYIPVQNIAEIITEIKAKKILYLG
                     NEKNISFSLEDDIIFNKNFLPFHANGMKFTAYLVDNTEYSSTYYSIGGGFVVVEERVN
                     AKKKLEIKCAFPFPIQNAEELLNYTEVHNKSISDIVYENEKSMRPEAEIHSELMRIWN
                     TMLECMYIGCHSEGILPGGLHVRRRAFDMHQHLIGLAHYNSPQTWLEQIRLTEVKFRQ
                     ILKWVSCFALAVNEVNAALGRVVTAPTNGSAGVIPAVLMYYLVIENHNAGEKEIKQFL
                     MVTGEIGSIFKKGSTISAAMGGCQAEIGVSSAMAAAALCELMGGTPAQVLMAAEIAME
                     HHLGLTCDPIGGLVQIPCIERNTMGALKAINAAELALETDPKNAKVPLDKVVDTMWQT
                     AKDMNNKYKETSEGGLAVAVNMADC"
     gene            9969..11039
                     /locus_tag="PLQ70_02680"
     CDS             9969..11039
                     /locus_tag="PLQ70_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQE33547.1"
                     /translation="MEPIKTNSSSYNFEKIVDIIIRLGVLAVLLMWCFDILKPFVLIL
                     IWAVVIAIAIFPVYSFLSKIFRGKKIIAVLVLILLMLSIIMVPSGLIVYSLYDGVNHF
                     QELFNTGEHLIPPPGGTTANWPIITKPIVDIWQMASDNLQDVVLKYSDQIKEYGAIIL
                     IAFASVSKGIVSFIISIIIAGVLLVYSDSSADVSQKIFRKLVGSNSDHFAEISVVTIR
                     NVVKGILGVAFIQATMASLGFFIAGVPFAGLWTILCLILAIVQIGVGPIVIPVIIYMF
                     SVSDTTTAVILAIWLGITLLIDNILKPILLGRNAPAPMLVIFLGSIGGFLYNGFIGLF
                     LGAIILTIGYKLFMTWLETDNL"
     gene            11399..12181
                     /locus_tag="PLQ70_02685"
     CDS             11399..12181
                     /locus_tag="PLQ70_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQE33548.1"
                     /translation="MDLVGLIVAGLVVGFIVGMTGVGGGSLMTPILLWFGIPPSTAVG
                     TDLLYAAITKSGGVYVHNKKKNINWVITGWLTLGSVPAALLTLWILHSLKADLEVMNK
                     LIKYSLGWALLFTSVAIVFKKKLLVFSQKHAGDKFHTESKTQNLLTVAIGVMLGATVT
                     LTSIGAGALGTVTLFFLYPLLPTPKLVGTEIAHAVPLTLVAGLGHASMGNLDFNILGH
                     LLMGSLPGIYLGSLLSGKLPDFYLRNAIAIMLFYVGFNLVVK"
     gene            complement(12203..13753)
                     /locus_tag="PLQ70_02690"
     CDS             complement(12203..13753)
                     /locus_tag="PLQ70_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQE33549.1"
                     /translation="MKRAIKYYRIIWFKHDLPAGLSVFLVALPLCLGIALASGAPLYS
                     GILSGIIGGLVVSVVSGSQLAVSGPAAGLSTMVSAAILSLGDYRLFLLSVIIAGLFQI
                     VLGLLKLGAIANYFPSSVIKGMLAAIGIILVSKQIPLALGYDKPDFWTSGFIQLFSAN
                     NFFGNIENFNNHITRGAILITTFSLVVLLTLQQPFAKKLKIIPAPLLVVVIGVITNIV
                     FTHLTSEFSLKPTQLVSIPSNFLESIAFPNFSKLFSTIEIWKNGILIGLLASLETLLC
                     IEAVDKLDNRNRITPVNRELIAQGIGNMTCGLLGAIPITAVVVRGSANVDAGARTKLS
                     AFTHGLFLLFAVLMIPFLINKIPYASLAAILLVTGFNLTKPSLYSNMWQLGRKQFIPF
                     LITIVVILSTDLLIGVSIGLLISIYYIIQSNFKIEYHLNQTKFQGIETQYIKLNHNVT
                     FLNKVKLRKTLDEVPEYCVLTIDGSESRQIDYDILEIISEFQRKAHDRHIELHLINIE
                     SVKIMAVH"
     gene            complement(13847..14236)
                     /locus_tag="PLQ70_02695"
     CDS             complement(13847..14236)
                     /locus_tag="PLQ70_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HQE33550.1"
                     /translation="MFTIEQIKEAHSKVKSGADFPAYIQDLIDLGVLSYDTFVNDGHV
                     EYLGSHTFRVAQTDTYDSITIAPIANKERFIEFLVMHQDGQTDYLTFCHHAAQCGIAK
                     WNVNIIEMTCTYFDSTDAPILIEKIPS"
     gene            complement(14247..14681)
                     /locus_tag="PLQ70_02700"
     CDS             complement(14247..14681)
                     /locus_tag="PLQ70_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQE33551.1"
                     /translation="MATFSKQLSFRWSDLDPNFHLRHSAYYDFGAQHRIEILEELGLT
                     LRVMQKEHIGPVLFREECVFRKEINLSDVIIMQTKMAKMNADASRWSIVHEFYREEVL
                     CAIITVDGAWLDTKLRKLASPTPQIVNDALSVFPKTTTFTTL"
     gene            complement(14725..14937)
                     /locus_tag="PLQ70_02705"
     CDS             complement(14725..14937)
                     /locus_tag="PLQ70_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQE33552.1"
                     /translation="MAIVVNLDVMMAKRKISLNDLSAKVDLTLSNLSILKTGKAKAIR
                     FSTLEAICKALDCQPGDILEYNNPEK"
     gene            complement(14941..15477)
                     /locus_tag="PLQ70_02710"
     CDS             complement(14941..15477)
                     /locus_tag="PLQ70_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HQE33553.1"
                     /translation="MKTKKNVVFIILNIITWIIFVGLSIEAGALIVNFVFSIFKPEMV
                     QNLYNKMDLSELYNQSWMVFYGMYGFVLFISILKCILFYIVIMLILKLNLEKPFSTFV
                     SAQITKMSYLTLSIGLISYIASEIAKKMQQTGYEIEPLNQFWVDSEAFILMAAIVYVI
                     ALIFKKGVELQSENELTI"
BASE COUNT         5010 a         2601 c         2681 g         5309 t
ORIGIN      
        1 tccgtgggta aaggaatcgg gaactacttg tccttgcatt tttttctgga tggcgtcgtc
       61 accaaccgca tttgctgcac tcaacgcttc ttcgatatcg ccaggttcta gcattgcgtt
      121 ttgtttttcg ttgtaatgtg tccaaagtcc agcgtagaaa tcggcttgca attctagagc
      181 aactgataat ttattggctt cggcttcact ttttccttct tgagcttggc gcattttggc
      241 cgaagttccc aacaaggttt gaatgtggtg tccgtattcg tgtgccaaaa cataggctac
      301 agcaaaatcg ccaccttcgg ctccaaattt tgttcttaat tcttcaaaga aagtcatgtc
      361 catataaata attcgatctc caggacaata gaacggacca gaagctgaac tagctccacc
      421 gcaagccgtt tgcacttgtc cgctgaataa aactaattta ggagcttcaa atttcaggtt
      481 gttttctgca aatatttttg tccaaacatc ttcattatct gccaaaagcg tttttacaaa
      541 ttcgccttct ttgacttctt ctggagttaa ggctctttcc tcagttggtg cgccttgttg
      601 tccttgggtt tgttgtaaaa tattgcctat catttgacca ttttcgcctc caaaagcatt
      661 taacaacaag attacaattc ctaaaactcc accgccaatt aaagtttttc caccgcctga
      721 cattcctctt cggtcgtcaa cattatcact ttgtcttctt ccaatccatt tcataaataa
      781 agagtttttt agttcaacaa taatagttgt tatttaattt tcagttccag taattttatc
      841 aatttcactg gtttcatcaa ttcctatgat gtttttataa atggcataat tcatcgaata
      901 tataaatggc caagtgaaaa atattccgat gcacagacca aaaattccaa gcgcaactcc
      961 tatccctgct acaattatta atccaagaag cactaaaggt tgtttagaaa tcagtaatat
     1021 acttgatttt atagcatcga ttgcgtttaa ttttgcaaaa acaattaatg gaattgtcaa
     1081 aaaggtaaga aacgatacca acaaagaaat tacagttcca acaaaactta atcctgcata
     1141 ttctagaagc aatgctgctc cagtactaat gaatccagcc aaaagggttg ataagaaaat
     1201 agcataaaaa taggggtatt tgtaataaga aaacatggtc gaaacatgaa attcttcgcc
     1261 tttttcgccg caatcggcca tttttagaaa gcctgccatg aatggattta tgagtgctga
     1321 aaataaaagt aagcccatgt ttattgggat agcaatatcc agaggcatta ccttgttttc
     1381 tgaaatttgt ttcattttat tgccaaattc ttccatatgt tcaaccccaa taaaggcaac
     1441 taaactaacc ccaaaaacga ttgaaattaa aatccaaaaa atcaaaatca ttaatccagc
     1501 atacaaagca atttttttat aattctcgaa agcactttcg aaaacgatac caaaatctag
     1561 ttggtatcca ttagcgcata aattttctat ttgatctttt tgtgtatttg tattttgcat
     1621 tttttgtggt ttaaaaagtt gttttttggg ttaaatccag tctaatattt tttttatcgt
     1681 tgctaatttt cctttgtaag gagcataacg aaaaggtaaa tctaacaaat tagattttat
     1741 tagaatactt tttttgtgag aaaatgtgtc aaaacttaaa attccgtgat aagcgccaat
     1801 tccgctgtgg ccaacaccac caaatggtaa tcttttattc gaaaagtgaa ccattgtatc
     1861 attgacacaa ccgccgccaa aagaatattt ttgtatcaaa tgttttgtaa aagttctatt
     1921 ttcagaaaaa aggtaaaaag ccaaaggttt ctcatatttt gaaattacgg catcaatttg
     1981 tttttccttt tcataggtga taatgggtaa aatggggcca aatatttcgt ctttcattag
     2041 ttcactgtca attgaatttt cgtcaattag tgtaggagcg atatagttgt ttttgatgtc
     2101 attttgtcct ccaaaaatca cttttttttg gtcgatcata ttaattaatc gttggcagtt
     2161 ttttgtattt atgattcttg caaaatcagg agaatcacta ggattatcgc cataagcttt
     2221 gatgatttcc tctttcatat agtcaacaaa atggcttttc atgttttttt gaatcaaaat
     2281 ataatcgggt gctatgcaag tttgtccagc attcataaat tttccccaaa cgattctttt
     2341 ggcagcgagt ttcaaattgg cagtttcatc aataatacaa ggattttttc cgcccaattc
     2401 taatgttaca ggagtaagat tttcggctgc agctttggca actatcttcc caacggctac
     2461 acttcctgta aaaaagatgt aatcccatcg ttctgaaagt aattgttgcg acacctcaat
     2521 tcctccttgg ataacagtaa tatccttctt atcaaatact ttcgagatta tttttgtaat
     2581 aatttcagaa gttttggggg tgagttctga gggttttaga acgacttcat ttcctgctgc
     2641 aaaggccgaa attaaaggac aaagcgctaa ttgaaaagga taattccaag gcgaaataat
     2701 taaaactttg ccataaggtt ctttataaat ataatctttg gaagggaaat tcaaaagcga
     2761 aggaaatact cttctgggtt tggcccattt atttatgttt tttatagtct cttttagttc
     2821 tgagattaca taacctgttt cagtgagaac tgcttcaaat gcaggttttt taaaatcgaa
     2881 gtataaggca tcaatgattt cattttcgtg gatgagtatg gcattgagca actttgttaa
     2941 agtttctttt ttgaaattaa tgttggtttt agattccata atttaaaact taaatgaagt
     3001 tttaaagata catttttttt caaattatat ggctttccaa atcacatcat ttggtacagg
     3061 agcaacaata cttaaatttt ctttggaaac gggatgaata aaaaccaatt ttcgagcgtg
     3121 aagatgaatt cctccatcgg gattagaacg gtcaaatccg tatttcaaat cgccttttat
     3181 aggacatcca atagccgaaa gttgtgctcg aatttgatga tgtcttccgg tatgaagatt
     3241 gatttctaat gcaaaataat tgttgagttc cttgatgatt gtataatcta aactagccaa
     3301 tttactatcg ggaacttctc tagaatgtgc ttttgaagta ttgttttttt cgtttctttt
     3361 gagataatga acaagtttgt cttgagtatt ttcaggtttg ttttttacaa cagcccaata
     3421 ggttttctgg gtttcgcgat tgctgaacaa ttcattcatt cgagacaaag ccttgctggt
     3481 tctcgcaaaa acgacgattc ctgttgtggg tctatccaaa cgatgaacca ctccaagaaa
     3541 aacttcaccg ggtttgttgt atttgtcttt gatgtattct tttacgactt cggataaggg
     3601 tttatctccc gttttatctc cttgcacaat gtcgcctaca cgtttgttaa ccacaataat
     3661 atgattgtct tcgtgcagaa tttgcaagtt gtttttatca gaaagtattt tcattggttt
     3721 ggaagaattc cttatttcga ttcgaaaaca gaggtgttgt tattatggtc gattaggttc
     3781 attttttcaa gactttcttt tgagacttgt tctaattttc gggttgggat tacaaaaaat
     3841 aattttttgt tcaaactcaa aaccgaatcc acttcttttt gattgataag tacttgctct
     3901 tttccgttgg aataaaattc tcccgagtaa tttttttgtt tatttacaac agaatcgttt
     3961 tcgactttaa cgaaataata caaaggaatt tgtttgtttt cagtattaac tttcagtttt
     4021 tctaagaggt atttgtcgga attcatgata taagaaacat cgggatatcc actcagtttt
     4081 gtgaataaat cttttggcaa tagtatggca acgcttgcaa taacaaaaat tgctgtaggg
     4141 aaaaacaatc cgattcgaat gagtgttttt ttactgtcaa attcattcca ataatgaacc
     4201 atcaatagca tcatcgggaa agtcgtaggt aaaatgtagg tgtgtagaat attttttgaa
     4261 aaagtaaaaa agaaaggcaa ccacaaaagc cataaaacca gaaatgaaac ccaaggattt
     4321 tttaaaatag tagttctgtt tttccataat ttgtaaacaa caattggaaa ccaaggaaaa
     4381 gcaaatgcta tcatatatac ccaaacaatt cccattggaa catattttgg attgccgtat
     4441 aaatccccat tccaacctgg aactaagaag cgtttgtaat gttcgccaac aaagaaataa
     4501 ttggtaaatc caggtgattt ttgctcaatt aaataatacc aaggtaatgc aattgccaca
     4561 acaataagta ttcctaaaat aatgggcaaa cgagaaaaaa cagcttttaa acggtttaaa
     4621 tctagcaagc accaaacaaa aagaggaggg aaggtaagta ccattatcag cggtcctttg
     4681 gctaaaaaac caaggcctaa agcaataaaa aatagataat tccaaatggt tttttcggta
     4741 ttgtttacca ctttccagaa agaaagcatc attattgtga tgcatacaaa caaagcacta
     4801 tcggtagaaa caacaccagt gtgaattaaa aattctggca ttgttagtaa tataaaacca
     4861 ggaagataaa aagattttcc ttcttttttg gctaattttc cagcaataaa tagaagcaat
     4921 acactcaata gatacgatgg aaatcttgcg gaaaattcgt ttgcaccaaa aatttcaaaa
     4981 cttcctgccg aaagccaagt tgacaaaggt ggttttgccc aaaacgggaa accataatca
     5041 atttgaacta caatccagtc tttggtttca tacattaatc ttgctatttc ggcatatcgc
     5101 gcttcggttt tgtctagaag tgggatggca aaagtgagta ttaatcggaa gattaaaatg
     5161 gttataacag agaagcaaag tataaaatta gtgtctttta atttttttga aatcatttgc
     5221 tatgcgtttg gattggataa attctttgca atttttttat attttctttg aatttcaaat
     5281 aagtcaaacc aaagtttgaa accgtaagtg aattttactt tagaatcgcc tttttcgacc
     5341 cataatttca aaggaacttc tatcattttt ttgatagctt tttctttgcc gaatagatga
     5401 atcattcggt aaaaaatctc tacatcaaac agccatctcg agataaattc tttttcgaat
     5461 actttttctg aaatttctct tgtaaaaagt ttcgaaccac attgggtgtc ataaaccttg
     5521 atgtctagga tgttcgagat aaatgttgcc acaactcttc ctatcaaaaa acgactgttt
     5581 tcgcgttcaa taatagagcc aatttttcga attcgagaac caaagctaaa cacaatttcg
     5641 ccttgcaaat aatttctaag ttcaataaat tcttcgagag ttgttgctaa atcggcatcg
     5701 agataaccaa tatagcgatg ttggaaatga aggttgcaat gattaattcc ggctcgaaca
     5761 gcttcggctt ttccagcatt tttttcgagt gaaaggattt gaatttgagt aggatgcttt
     5821 tctttcaaaa cagtcaaaac agcaagagta ttgtcttttg atccatcgtt tacaaaacaa
     5881 attaatacct ctggattgtt attcaaaaag ttggaatatt cggcatttga aattcctttt
     5941 tcctcattat aacagggaat aacaacggtt gtattgggta taattgtcat ataaaaaata
     6001 ttttttctac aaatcttttc tgttttctaa tctagtattg ttctttttca tttggaaaat
     6061 cttgcgattt cacatcggct acatattgtc caattgccga ggtcattcct tcgtataaat
     6121 tcatataacg acgcaaaaat cttggactaa attcattgtt cattcccaac atatcgtgaa
     6181 tgactagtac ttgtccgtca acaccgccac cagcaccaat tcctataact ggaatcgaaa
     6241 tacttttggc cactttctgg gctaattcag caggtacttt ttcgataact atagcgaaac
     6301 agccaagttt ttctaagagt tttgcatctt caattaattt ttcggcttct tcttcttctt
     6361 tagctcgaac ggtgtaagtg ccaaatttat agatagattg tggtgttaaa cccaaatgtc
     6421 ccataacagg aattcctgcg tttaaaattc ttttgatgga ttctttgatt tcgctaccac
     6481 cttctagttt tacagcgtgt ccgccacttt ctttcataat tcggatggaa gagcgcaaag
     6541 cttcttttgg atcggattga tagcttccaa aaggcaaatc gaccactacc aaggcacgat
     6601 aaatagcacg aaccacacta gatgcgtgat aaatcatttg gtctaaagtg atgggtaaag
     6661 tcgtttcgtg ccccgccatt acattcgacg cagaatctcc taccaaaatt acgtcaacac
     6721 ctgcggcatc aacaattttt gccattgtaa aatcataagc cgtaagcata gaaatttttt
     6781 gcccatcggc tttcatttct attaatgact ttgttgtgat tcgtttgtaa tcttttttag
     6841 cggttgacat tatttttagg aattatagat ttgaggtgca aaattagtca atttatctaa
     6901 aatctattgg aattacagtt tttaaatgat taaatcctaa ataataccct tttgcaatta
     6961 gtgtttaatc gcaatttact gttgttaatt tctgtttaaa cttcttagat ctatcgcaaa
     7021 agcaattgct attccaaagg tataaaagca caaatcgctc caaagaaaac cttgccctaa
     7081 aacataatgt cccaaggttg tatttcgaat agctaaaatc caatcggctc tataaagttg
     7141 ttgaagctcg atgacaaagc aaaataataa cgcaagaatt gcagcttttt tggtatttga
     7201 attgacaaaa agaatgcgca tcccaaagta aatcatgcaa gcatataaac catcaccaat
     7261 aaaggttgga acgttatcaa ttttccttga aattacaccc ataatcatta caattatagt
     7321 taaggtaaaa tagtataatt gattgttttt tagcatgatg aaatttaaat cttaaaccca
     7381 agttaaaacc gcccataaag tcgaaaacga aataataatc caaagaacaa ttccttctat
     7441 taaaggttta taacccacag aaactaatac ttttctcgac aaaccacagc caataaggaa
     7501 caatgttagg gttaaagttg cttttgcaaa tccagtgatg taatggctaa aattttgtac
     7561 aaaaggaaga taagtattgg ccatcattac taaaataaat aacccaatga aatacggaat
     7621 tttgattttg gtatctttat ttttgaatac aaaagttgat aaaaacgcaa taggtataat
     7681 ccacaatgct ctagctaatt tgactgttgt tgctacttca agtgcttgag aaccatattt
     7741 tccagcagct cctacaaccg aactcgtgtc gtgaatggcg atggcgcacc acattccaaa
     7801 ttgggtttcg gataaattca aatagtgacc gatatacgga aatgcgacta aagcaattga
     7861 attaagaatg aaaatcgttc ccaaagctac cgaaatttgt ttttcattgg ctttgattac
     7921 aggcgaaatg gcagcaatag cgcttccgcc acaaatggcg gtccctgtgg caatcaagta
     7981 ggatattttt tcttctattt ttagaatttt acctaagaaa atcccaatta agatggttcc
     8041 aataattgaa gcgattgtca aaacaattcc ttctttacct gcttttaaag cactatttac
     8101 attcattcca aaaccaagtc caacaactga aacttgtaat aagatgtggg tggctttgtg
     8161 atttaaattc aaatagggat gcccgataaa ttgagcaata atcaatccca ttagcaatgc
     8221 gattggtgga gaaattaaag gagataagca taaaaccaca aaaaacataa aaatgacctc
     8281 acgcgtggtg atggttttgt ctaagatgct ttttttggtt tgattttcat ttttcattct
     8341 gattttggtt taagagtgca aatgacagca aaaatagctt agaaatgaaa ttgaatttca
     8401 caaaaattag tctatttgta attgtttttg ccaaatattg ctctagcaat ccgccatatt
     8461 taccgcaaca gctaagccgc cttctgaggt ttctttgtat ttattgttca tatccttggc
     8521 cgtttgccac atcgtgtcaa ctactttatc caaaggtact ttggcgtttt ttgggtcggt
     8581 ttcaagagct aattcggcgg cattaatggc ttttaaagcg cccattgtat ttctttcgat
     8641 gcaaggaatt tggaccaaac caccaatagg gtcacaagtc aatcctagat gatgttccat
     8701 ggcaatttcg gcagccatta aaacttgtgc aggagttccg cccatcaatt cgcataaagc
     8761 tgctgctgcc attgctgatg aaacaccaat ttcagcctgg catccaccca tagcagccga
     8821 aatggtagaa ccttttttga aaatgctacc aatttctcca gtaaccatca agaattgttt
     8881 gatttctttt tcgccagcat tatgattttc gataaccaaa taatacatta aaactgccgg
     8941 aattacgcca gcacttccgt ttgttggagc ggttacaaca cgacctaaag cagcgttgac
     9001 ttcgtttaca gccaaagcaa aacacgaaac ccatttcagt atttgtctga atttcacttc
     9061 tgtcaggcga atttgttcta accaagtttg aggtgaatta taatgagcta aaccaatgag
     9121 gtgttggtgc atatcaaaag ctcttcggcg cacgtgcaat cctccgggta gaatgccttc
     9181 ggaatgacaa ccaatgtaca tacattcgag catggtgttc caaattcgca ttaattcaga
     9241 gtgaatttca gcttcgggac gcattgattt ttcgttttca taaacaatgt ctgagatgct
     9301 tttgttgtga acttcggtat agtttaataa ttcttcagca ttttgaatgg gaaacggaaa
     9361 agcgcatttt atttccagtt tctttttagc attgacacgt tcttccacaa ctacaaatcc
     9421 acctccaata gaataataag tcgatgaata ttcagtgttg tccactaaat aggctgtaaa
     9481 tttcatgcca ttggcgtgaa aaggcaggaa gtttttattg aaaataatgt catcttctaa
     9541 cgaaaaagag atgttttttt catttcctaa atagagtatt tttttagctt taatttcagt
     9601 gataatttcg gctatatttt gaacaggaat atattcagga tcctgaccag aaagtcctag
     9661 cataatggct aaatcagttg cgtgaccttt tccagttaaa gaaagagagc cgaataaatc
     9721 tactttgatg tgatgtacag tttcaaaaat tgtgttggca cgaagttctc ccaaaaatcg
     9781 ttcggcagct cgccaagggc caagtgtgtg agaactagat ggtccaacac caatcttgag
     9841 catatcaaaa accgaaatgc attcttccat aagagaaaat ttagtagaac aaatatagaa
     9901 gattgcgctc tattttattg ctgtactttt atatatttac agaatgttaa tttatcaatt
     9961 tttaaaaaat ggaaccgata aaaacgaatt cttcaagtta taatttcgaa aaaattgttg
    10021 atataatcat acgtctagga gtattagcag tattattgat gtggtgtttt gatatattaa
    10081 aaccatttgt attaattctg atttgggctg tagttattgc tattgccatt tttcctgttt
    10141 atagtttttt gtcaaagatt tttagaggaa aaaaaattat agcagtttta gttttgattt
    10201 tgttgatgtt aagtattata atggttccaa gcggacttat cgtttattca ctttatgatg
    10261 gggtaaatca ctttcaagaa ttatttaata ctggagaaca tttgattcca ccgccaggag
    10321 ggactactgc aaactggcca attattacaa aaccaattgt tgatatttgg caaatggctt
    10381 ctgataattt gcaggatgta gttttgaaat attcagatca aataaaagaa tatggtgcca
    10441 taattttgat agcttttgct agtgttagca aaggaattgt atcttttata atttcaataa
    10501 ttattgctgg agtcttatta gtctattccg attcatctgc agatgtttct caaaaaattt
    10561 ttagaaaatt agtgggttct aatagtgatc attttgcaga aatttcggtt gtaaccattc
    10621 gaaatgtggt taaaggtatt ttaggagttg cgtttattca agctactatg gctagtcttg
    10681 gttttttcat tgcaggagtt ccttttgctg gtttgtggac tattttgtgt cttattttgg
    10741 caattgtgca aataggagtg gggccaattg ttattccggt tatcatttat atgttttctg
    10801 tttctgatac tactacagca gtgattttag ctatttggtt gggaattaca ctattgattg
    10861 ataatatttt aaaacctatt ttattaggga gaaatgctcc agctccaatg ctagtgattt
    10921 ttctaggttc gataggtgga tttctttata atggatttat aggcttgttc ttaggagcta
    10981 tcattcttac tattggttat aaacttttta tgacttggtt ggaaactgat aatttgtgaa
    11041 acaattgtgt ttgaatttcc ttgaatttta tctttttatt agattaattt aatttttttt
    11101 tcgagtgaaa aaagctactt tttaaaatta aagttatttt ttttcaaata gtaaggacta
    11161 ttttaattct acatattcta tagggttatt tatgaatttc tactaaaaca cctaaaaaaa
    11221 ttaattttct tcggatttta tcaattttta aagcagtatt agtggtaaat tcgattttaa
    11281 attaaatgaa ttattttttg tttgagtcta aaatcattat atacagtatg aagaatttac
    11341 aaactatttg gattatatag tgtttttact atttttgcca aaaaataata taatatctat
    11401 ggatttagta ggattaatag ttgcaggatt agttgttggt tttattgttg ggatgacagg
    11461 cgttggtggg ggatcattga tgacaccaat tttattgtgg tttggaatac caccttctac
    11521 tgctgtaggt actgatttgt tatatgcagc tattactaag tcgggtggtg tttatgttca
    11581 taacaagaaa aaaaacatca attgggttat aactggttgg ttgactttgg gtagtgttcc
    11641 tgcagctttg ttgactttgt ggatacttca tagtttgaaa gcagatcttg aagttatgaa
    11701 taagcttata aaatatagtt tgggatgggc attgcttttt acatcagtag ctatagtttt
    11761 taaaaagaaa ttgttggttt tttcacaaaa gcacgctgga gataaatttc atactgagag
    11821 taaaactcaa aatttattga ctgttgccat tggagtaatg ttaggagcaa cagttactct
    11881 aacttcaatt ggagctggag cattaggaac agtaactttg ttttttctat atcctctttt
    11941 gccaacgcca aaattagtag gaaccgagat tgcacatgca gtaccactaa cactagttgc
    12001 aggattggga catgcttcaa tgggaaattt agattttaat atattagggc atttacttat
    12061 ggggtcattg ccaggaatat atttaggaag tttattgagt ggaaaattac ctgattttta
    12121 tttaagaaat gctatagcta taatgttgtt ttatgttggt ttcaatctag tagtcaaata
    12181 aagagtagat tatttttttt gactaatgaa ctgccattat ttttacactt tcaatattaa
    12241 tcaagtgtaa ttctatatgt cggtcgtgag cttttctttg aaattcgctt atgatttcta
    12301 aaatatcata atctatttgg cgactttcac taccatcaat ggttagaacg caatattcag
    12361 gaacttcgtc taatgttttt ctcaatttaa ctttgttcag aaaagtaaca ttatgattga
    12421 gtttgatgta ttgcgtttcg attccttgaa atttagtttg attgagatgg tattctattt
    12481 taaaattact ttgaatgata taatagatag aaatcaaaag tccgatgctt actccaatca
    12541 gcaaatcggt agataaaatg accacaatgg taatcaaaaa aggaataaac tgtttgcgtc
    12601 ccaactgcca catattgctg taaagacttg gttttgtcaa attgaatcct gtgactaaaa
    12661 gtatggctgc caatgaggca taaggaattt tattgattaa aaaaggaatc attaaaacag
    12721 caaataacaa aaataatccg tgagtaaatg ccgataattt agttctagcc ccagcatcaa
    12781 cattggcaga acctcttacc acaaccgcag ttatcggaat tgctccaaga agtccacaag
    12841 tcatattgcc aattccttga gcaataagtt cccgattcac aggtgtaatt cggttgcgat
    12901 tgtctaattt atctaccgct tcaatacaaa gtagggtttc taaactagct agcaagccga
    12961 taagaattcc gtttttccaa atttcgatag ttgaaaacag ctttgaaaaa tttggaaatg
    13021 caatactttc taaaaaatta gaaggtatgg aaaccaattg agttggtttt agcgaaaatt
    13081 cggaagtcaa atgggtaaaa acaatattgg taatgactcc aattactacc actaataaag
    13141 gggctggaat aatctttagt tttttggcaa aaggttgctg aagagtaagt aaaacaacca
    13201 aagaaaaagt ggtaatcaga atggctcctc gggtaatatg attattgaaa ttttctatat
    13261 ttccaaaaaa gttattagcc gaaaacaatt gaataaaacc actggtccaa aaatcgggtt
    13321 tatcatagcc taaagccaaa ggaatttgtt tagaaactaa aattattcca atcgcagcaa
    13381 gcattccttt gataaccgat gaaggaaaat aattagcaat tgcgcccaat tttagcaaac
    13441 caagtacaat ttgaaataaa ccagcaatta ttactgataa caagaagagt cgataatcgc
    13501 caagcgaaag aatggcagcc gaaaccatag ttgacaatcc tgctgcaggg ccagaaacgg
    13561 ctagttgcga accactaaca actgaaacca ccaatccacc aataatgcca gagagtatgc
    13621 cagaatatag aggcgctccc gatgctaaag caattcccaa acacaatggt aaagccacta
    13681 gaaatacaga aagtccagct ggtaaatcgt gtttgaacca aatgattcta taatatttta
    13741 ttgctcgttt catagcttaa aaaagttacc caaaggtaag atttttgtgt aaacagcata
    13801 attataaatc gattgatttg caatacagaa agtaaatact aattttttaa ctcggaattt
    13861 tttcaattaa aatgggtgca tcggttgagt caaaataagt gcaagtcatt tcgataatat
    13921 tgacgttcca ttttgcaatg ccacattggg ctgcgtgatg gcaaaatgtg aggtaatcag
    13981 tttgtccatc ttgatgcatg accagaaatt ctataaaacg ttctttgtta gctataggag
    14041 caatggtaat gctatcgtaa gtatcagttt gtgcaactct aaaagtatgg ctgcccaaat
    14101 attcgacgtg gccgtcattt acaaaagtgt catagcttaa aacgcccaaa tcaattaaat
    14161 cttgaatgta agcaggaaag tcggcaccag attttacttt ggaatgagct tcttttattt
    14221 gttctatggt aaacataatt caataattat aaagtggtaa aagtagttgt ttttggaaaa
    14281 acactcaatg catcatttac gatttgtgga gtaggagaag ctaattttcg aagtttggtg
    14341 tccagccaag ccccatcaac agtgataatg gcacataaaa cttcttctct ataaaattcg
    14401 tgaacgatgg accagcgcga agcatcggca ttcattttcg ccattttggt ttgcattata
    14461 atcacatcag aaaggttgat ttcttttcta aaaacacatt cttctcgaaa caatactgga
    14521 ccaatatgct ctttttgcat tactctcaag gtcaatccta attcttccag tatttcaata
    14581 cggtgttgcg ctccaaaatc ataatatgca ctgtggcgta agtgaaaatt aggatctaaa
    14641 tccgaccatc gaaaagaaag ttgtttgcta aatgttgcca tcgttttttt tgctaaaatt
    14701 atggataaaa gttgaaaaga tattttattt ttcaggatta ttatattcta aaatatcgcc
    14761 gggttggcaa tccaaggctt tgcaaatggc ttctaaagtg ctaaatcgaa tggcttttgc
    14821 tttgcctgtt tttaaaatcg aaagattaga taatgtcaaa tcaacctttg cagaaagatc
    14881 gtttagcgaa attttgcgtt tcgccatcat tacatctagg tttactacta tcgccataat
    14941 ttagattgtt aattcgtttt ccgattgcaa ttcgactcct tttttgaaaa taagggcaat
    15001 aacataaact attgctgcca tcaaaataaa ggcttcacta tctacccaaa attgatttaa
    15061 gggttcaatt tcgtaaccag tttgttgcat tttttttgct atttcactag caatataact
    15121 tattagtcca atagaaagag tgaggtagct catttttgta atttgagccg aaacgaaagt
    15181 actgaatggt ttttctaaat tgagttttag aatgagcata atcacgatat aaaataaaat
    15241 gcatttgagg atcgaaatga ataaaacaaa accatacata ccgtaaaaaa ccatccaact
    15301 ttgattgtac aattcgctta aatccatttt attgtataaa ttttgtacca tttcaggttt
    15361 gaaaatactg aatacaaaat tgacaatcaa tgcgccagct tcaatagaca agcctacaaa
    15421 aataatccaa gtaataatgt ttaggattat gaacactaca ttttttttgg ttttcataat
    15481 gagttgattt ttaaaattat tggacaaata taatatatat ttattgaata acaataaaaa
    15541 tatattaata ttaaataatt ttttttttgg ataaaaaaat ggtagtgaaa gtttggatta
    15601 t
//
LOCUS       DAOSNN010000028        15328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_3881_length_15328_cov_5.011524, whole genome shotgun sequence.
ACCESSION   DAOSNN010000028 DAOSNN010000000
VERSION     DAOSNN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 15328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15328
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3881_length_15328_cov_5.011524"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..721
                     /locus_tag="PLQ70_02715"
     CDS             89..721
                     /locus_tag="PLQ70_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQE33554.1"
                     /translation="MNEIKLIIADDHKLFREGLEGLLKKHDNIKIIKSVADGDEFIDI
                     VKNQLVADIVLLDITMPNRDGFQVLKELKTLNSTIKPIVISMYDDGNYIAKCAKLGAY
                     GYLLKNTDESELILAIKTVSKGKKYFSPEISEKMINFMSTQSISENVLSNKETEVLGL
                     LSKGLTTNEIAAKLYVSSRTIETHRANILKKLEVKNTAELIKKAAEMNLI"
     gene            837..1160
                     /locus_tag="PLQ70_02720"
     CDS             837..1160
                     /locus_tag="PLQ70_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010521866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33555.1"
                     /translation="MKTIALIFAVLGFSFSSIAQEESNVKKNDLKGPAYKNYKVWMDK
                     SEPLKLYSENNKKPLTGPAYKNQQVSNDSSKKDLVEVKISGGERQKLTGPAYKNYGPW
                     SRSTK"
     gene            1424..2554
                     /gene="chrA"
                     /locus_tag="PLQ70_02725"
     CDS             1424..2554
                     /gene="chrA"
                     /locus_tag="PLQ70_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HQE33556.1"
                     /translation="MENKAAIKDIAKLFLKLGIIGFGGPAAHIAMMQDEVVSKRKWLT
                     EQHFLDLIGATNLIPGPNSTEMAIHIGYEKGGWKGLIVAGLCFILPAVFITGFFAWGY
                     KQYGQLPEVQPFVYGIKPAIIAIILGAIFPLAKKSLKSTELILIGITVLAGSLLHFSE
                     IYLMFGAGFFALLLAYLRDKEQKNLSSFLPLTFIPISNTTLISVTNANLFWIFLKIGA
                     ILYGSGYVLFAFLDTELVATGLLTRHQLIDAIAVGQFTPGPVFSSVTFIGYQINGLTG
                     AIVSTIAIFLPSFVFVALLNPIVKKMRNSKLFSAFLDAVNVASVAIILAICFDMGKET
                     ITDWRTISIAILSIATAFGYQKINSAVIVLGGSLMGYLLTLL"
     gene            complement(2574..4142)
                     /locus_tag="PLQ70_02730"
     CDS             complement(2574..4142)
                     /locus_tag="PLQ70_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQE33557.1"
                     /translation="MPQELLFQTSPETATNELLLKQHIAKLIRVDVKEIQHISILKRS
                     IDARQKAVKFNLKVTIYLVGETIQEAKIELPEYKNVANALEVIVVGAGPAGLFAALQL
                     IELGLKPIVIERGKDVRGRRRDLKAINRDHIVDEDSNYCFGEGGAGTYSDGKLYTRSK
                     KRGDVTRILELFVAYGASPDILVDAHPHIGTNKLPQIIQDMREKIIEMGGMVLFETRL
                     TDILIKNNEVQGIVTKNGDTILANKIILATGHSARDIFELLDRKQIFIEAKAFALGVR
                     AEHPQSLIDSIQYSCDYRGQHLPPSPYSIVKQVGGRGMYSFCMCPGGVIAPCATSPGE
                     VVTNGWSPSKRDQATANSGIVVELKLEDFKPFAKFGALAGMEFQKSIEQKAWHLAGET
                     QKVPAQKMVDFTQKKVSSNIPKTSYVPGTTSVEMGEVFPGFLTQILREGFVEFGKSMR
                     GFLTNEAVLHAPESRTSSPVRIPRDAETLEHLQIKGLYPCGEGAGYAGGIISAAIDGE
                     KCALKIGEVLKATL"
     gene            4266..4505
                     /locus_tag="PLQ70_02735"
     CDS             4266..4505
                     /locus_tag="PLQ70_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33558.1"
                     /translation="MSRQIKLIWDFRGPTSAKTAEHHEIHLKEYIIAEKLSPNITGFQ
                     IINEMYATAFMVVTDETMIQVRDALKPHRGEVFEG"
     gene            complement(4578..6773)
                     /locus_tag="PLQ70_02740"
     CDS             complement(4578..6773)
                     /locus_tag="PLQ70_02740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HQE33559.1"
                     /translation="MNSNEIDIHRELKKYFGFSQFKGLQEQVIKSILNKENTFVIMPT
                     GGGKSLCYQLPALVQEGTAIVVSPLIALMKNQVDAIRSLSSENGVAHVLNSSLTKTEI
                     AQVKTDITSGLTKLLYVAPESLTKEEYVAFLQNVPISFVAIDEAHCISEWGHDFRPEY
                     RNLKHIIKQLGNVPIIGLTATATPKVQEDILKNLDTVDATTYKASFNRPNLYYEVRTK
                     TKNIEADIIRFIKLHKGKSGIIYCLSRKKVEAIAEVLQVNGISAVPYHAGLDAKTRAK
                     HQDMFLMEDVDVVVATIAFGMGIDKPDVRFVIHHDIPKSLESYYQETGRAGRDGGEGH
                     CLAYYSYKDVEKLEKFLSGKPVAEQEIGFALLQEVVAYAETSMSRRKFLLHYFGEEFD
                     TETGEGGDMDDNVRNPKVKVEAKDQVVKLLEVVRDTKHIYKSKEVVFTLIGRVNAIIK
                     AHRTDVQSFFGSGTDHDEKYWMALLRQVLVDGLLSKDIETYGVIKITQKGLDFIKKPE
                     SFMMSEDHEYNESEDEAIVTAAKGSGVADEVLMGMLRDLRKKVAKKLGVPPFVVFQDP
                     SLEDMALKYPISMDELINTHGVGEGKAKKYGKEFIALISQYVDDNDIIRPEDLVVKST
                     GANSINKLYIIQNIDRKLPLNDIASAKGLTMEALITEMEQIVNSGTKLNIKYWIDEML
                     DDDQQEEIHDYFMESHSDNIEEALKEFDGDYDIDELRLMRIKFMSEVAN"
     gene            6859..7824
                     /locus_tag="PLQ70_02745"
     CDS             6859..7824
                     /locus_tag="PLQ70_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQE33560.1"
                     /translation="MNTKENILILAKKTILSESESIAKLIDFIDFNFVEATQILFQSK
                     GRLIVTGIGKSAIIAQKMVATFNSTGTPSLFLHASEAIHGDLGMVQPDDIVLCISKSG
                     NSPEIKILVPLLKRFGNTLIAMTGNISSFLAKESQFVLNTTVDAESCPHNLAPTNSTT
                     AQLVMGDALAMCLMNLRNFSREDFAKYHPGGSLGKKLLLQVKDMLENSLKPMVTPDAP
                     IKKVILEISEKRLGATAVVENDKVIGVITDGDIRRMLNENDTFTHLTAKDIMTKTPKM
                     IQSTTMVVQALNILEDFSITQLIVVDHEQYKGIIHLHDILKEGIV"
     gene            7824..8645
                     /gene="tatC"
                     /locus_tag="PLQ70_02750"
     CDS             7824..8645
                     /gene="tatC"
                     /locus_tag="PLQ70_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQE33561.1"
                     /translation="MANKQLKEMSFLDHLEELRWLLVRSTIAIIIMAFFTYFVSDYLF
                     DTIIFGPTRPSFYTYRWFCELSHYLNFGESICIEELPFIIQNTEMEGQVNMFVWMCLL
                     AGFILAFPYILFQIWGFISPALYANEKKNAKFFIFISSLLFFLGVLFGYFIIIPMSVN
                     FVATFTVSEMVKNQFTLDSYIGMFKTSVIAGGLFFELPIIIYFLTKLGLVTPEFLRKY
                     RKYAIVIVLIVAAIVTPPDVVSQTIVAIPMLIIFELSVIISSIVYKRKIKNEQLS"
     gene            8629..8973
                     /locus_tag="PLQ70_02755"
     CDS             8629..8973
                     /locus_tag="PLQ70_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQE33562.1"
                     /translation="MSNLVKEFNDYRSKMNEKLLADNNKIVKRIFNLDTNAYADGALD
                     VRTKELLGLVASTVLRCDDCVKYHLENCYKEGISKEEMMEAMGIATLVGGTIVIPHLR
                     RAYEFWEVLENN"
     gene            9054..9794
                     /gene="lptB"
                     /locus_tag="PLQ70_02760"
     CDS             9054..9794
                     /gene="lptB"
                     /locus_tag="PLQ70_02760"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQE33563.1"
                     /translation="MKLRAENLVKTYKGRSVVKGVSVEVNQGEIIGLLGPNGAGKTTS
                     FYMIVGLVKPNMGNIYLDDLNITDFPMYKRAQQGIGYLAQEASVFRKLSVEDNILSVL
                     QLTNLSKEAQEEKMESLIEEFSLGHIRTNRGDLLSGGERRRTEIARCLATDPKFILLD
                     EPFAGVDPVAVEDIQRIVAQLKNKNIGILITDHNVQETLAITDKTYLMFEGGILKAGV
                     PEELVEDEMVRRVYLGQNFELRKKKIEF"
     gene            9837..10085
                     /locus_tag="PLQ70_02765"
     CDS             9837..10085
                     /locus_tag="PLQ70_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HQE33564.1"
                     /translation="MNNSNNWRDNDPDKQTAENWRKDPKNWIWNIFYYNKEDQRLFVS
                     KKIEWMGTTLNFANPKSVLVLVAMGSFLGMVLYFILKN"
     gene            complement(10089..10952)
                     /locus_tag="PLQ70_02770"
     CDS             complement(10089..10952)
                     /locus_tag="PLQ70_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HQE33565.1"
                     /translation="MRRKTTRSKHVIRRKPRKKGSFFSGKLLRFSIIGFFILLFIGLG
                     YHYRNGFLYYLGFKSYKLKYEQEQSKRISDIRNYQVLDNHKGKAIGIDVSEYQGEIDW
                     DLVQNVEEEYPVSFVLVRATAGNDGEDQQFEQNWKAVKKQKLIRGAYHYYRPNENSLE
                     QAKLFIKTVRLRKGDLPPVLDIEKLPKDQSLDSLKIGLRRWLKAVEAHYKVKPIIYTG
                     EKYYDDFLKKDFSDYLFWIANYNFYREKIEEDWLFWQFTEKASVSGIKGNVDVNIFNG
                     DVEQLRFITIE"
     gene            complement(10960..11661)
                     /locus_tag="PLQ70_02775"
     CDS             complement(10960..11661)
                     /locus_tag="PLQ70_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQE33566.1"
                     /translation="MKIKKHIPNIITLLNLFCGCIAVFFVAELNFEMAFFFVSLGIFL
                     DFFDGFFARLFKVAGPLGLQLDSLADMVTSGVVPGFVMFSMMENSSNPNHYFSFLGFI
                     IALGSCYRLANFNIDTRQTDSFIGLPTPANALFILSLPLVLKYSDSLVLLEILTNYWV
                     LLGITLFSAFILNAEIPLFSLKIKDFTFKKNALQIVFLSLCVLLLIFFQYLAIPLVII
                     TYVLLSVINNKFLKK"
     gene            11938..12924
                     /locus_tag="PLQ70_02780"
     CDS             11938..12924
                     /locus_tag="PLQ70_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HQE33567.1"
                     /translation="MNIGVDAAALGMSNAVTSSTNDVNSGYWNPAGLVNLEDHQLSLM
                     HANYFANIAKYDYIAYANTIDDESAWGISLIRFGVDDIMNTTELIDSQGNIDYNRISL
                     FSTADYGVTFSYARKLQVPGFQFGVNAKIIRRIIGDFASSWGFGFDAGLQFEKNDWKF
                     GLMLRDITTTYNVWSIDEAEYKKIADAIPGQNQELPESTEITAPKAQLGLSKKFIVRY
                     DYSILAAANLNMQFAKTNDILSTDFVSVDPALGFEFGYTDLVFLRAGVGNFQNIQQID
                     TTEKVGFQPNIGLGFKYKGIQIDYALTNLGNQSAALYSNIFSVKVDLGLFRN"
     gene            12944..14080
                     /locus_tag="PLQ70_02785"
     CDS             12944..14080
                     /locus_tag="PLQ70_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HQE33568.1"
                     /translation="MNSLSLKKIVLILFLIGTLSGFGQNIILSKSARTSVITCDTGNE
                     SYSLFGHTAIRISDPENNLDVVYNYGAFDFSTPNFVVKFAKGDLQYFAIANTFSDFMN
                     QYTYEQRSVFEQELLIPLAYKQKLFDNLTTVLSTSESYYTYKFIDKNCTSMVVDMLNK
                     TLGVKIIDKKFDTDKTYRSILFPYFDNFFYEKLGTSIIFGTKVDNYSDHIFLPFELKK
                     SLQETQFKNKRLCAQSKTLLEYKKQAPNSWWNNVYSYLLFLVFILILNKKIIDNFYLL
                     IMGLLGIFFIVMGFYSFHQELAMNYNVLLFNPALLVLLYFIVKKNKKWIVNLAVLNGI
                     FLGVYLIMMINKAHFLVVIPLVLTTLIIVSKIAYKNSKRIPVVI"
     gene            complement(14081..15238)
                     /locus_tag="PLQ70_02790"
     CDS             complement(14081..15238)
                     /locus_tag="PLQ70_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HQE33569.1"
                     /translation="MRSTILTASTAVLFYLLTPVFSAELPSNRFEIVVFYLTVLFTLI
                     FWRMFYVKLLASNRFVQNVLLVCHRDQVEGLISDLENSDPHYRIVAYVNYSYAGKKDE
                     FEDHYVQHVEIDDLVLFLNENRLFEIVVASQNTDDITSNLYMQLLHLLESGTTIREYT
                     QVYEDKTHRIPLQYITRDFYKFFPFNRTNNNQLYLLNVRIMEILISVIGLIIGTLLIP
                     FIAIGNAIGNRGKLFYTQERVGKNGEIFQILKFRTMINNAEKGGAVFSGSNDTRVTPF
                     GKFMRKTRIDEFPQFVNILKGEMAVIGPRPERPFFVKEIAEIMPFYETRHVIKPGLTG
                     WAQVNYSYGESMEDSLIKLQYDLYYIKHRSIFLDLDIVIKTFSTVLFYRGQ"
BASE COUNT         5115 a         2681 c         2614 g         4918 t
ORIGIN      
        1 aagaacgtgt agcgatttgt aatggcaaag tcacaatcaa tgcagcagcc ggaaatacaa
       61 cctttgaaat tgaattaccc atagaatcat gaacgaaatc aaattaataa tagctgatga
      121 ccataagctt tttagagaag gacttgaagg actactaaaa aaacatgata acataaaaat
      181 aattaaaagt gttgccgatg gtgatgagtt tattgatatt gtaaaaaacc aattggtggc
      241 agatattgta ttactagaca ttactatgcc caatagagat ggttttcaag ttttgaaaga
      301 attaaaaacc ttgaattcta ctataaaacc tattgtaatt tctatgtacg atgacggaaa
      361 ttatatcgca aaatgcgcca agttgggagc ctacggctac ttattaaaaa acacagacga
      421 atccgaatta atccttgcaa taaaaacggt tagtaaagga aagaaatatt ttagtcctga
      481 aatttctgaa aaaatgatca actttatgtc aacgcaaagc atcagtgaaa atgttttatc
      541 aaataaggaa accgaagttt taggattact atcgaaagga ttgactacta atgaaattgc
      601 tgcaaaacta tatgttagtt cacgaacaat agaaacccat cgagccaata tcctaaaaaa
      661 gttagaagtg aaaaataccg ccgaacttat taaaaaagca gcagaaatga atttgattta
      721 atgctctcaa aacaaaaagc atccgtattt atacggatac aaatagcgta gattcacata
      781 atcctaattt tctattaccg tatgtaattt tgtaaaaaat cataaaagta atagtcatga
      841 aaacaatagc cttaattttc gcagttttag gattttcatt tagctcaatt gcgcaagaag
      901 aaagtaatgt aaaaaagaat gatttaaagg ggccagccta taaaaattat aaagtttgga
      961 tggataaatc tgagccgtta aaattatatt ctgagaacaa taaaaaacct ttaactggac
     1021 cagcttataa aaatcagcaa gtttcgaatg attcttccaa aaaagaccta gtcgaggtta
     1081 aaatttctgg tggtgaaaga caaaaactaa caggtccagc ctataaaaac tacggacctt
     1141 ggagtagaag tacaaagtaa tcattaaaag ttttatgagg tgatgaaaca gccaacatat
     1201 cttaaatatc aaaatatttt agtcctgctt cggtgggact tttttttgtt ttaaagaaaa
     1261 actaaaatag tttctgatag ttactgaaaa ttaataattt tatcgaatcg atttccaatc
     1321 aaagaaaacc attgtgtggt caaatgcaat tctctactaa aaatcttaaa aaataattca
     1381 acggaagtag caatttggtc tgtggacaaa aacttaataa acaatggaga ataaagcggc
     1441 cattaaagac atcgcaaaac tttttctaaa attaggaatt attggttttg gtggtcctgc
     1501 tgcccatatt gccatgatgc aagacgaagt ggtaagcaaa agaaaatggc taaccgaaca
     1561 acattttctg gacttaatag gtgctacaaa tctaattcca ggtcccaata gtaccgaaat
     1621 ggcgattcat attgggtacg aaaaaggcgg atggaaaggg ctaattgtcg ctgggctttg
     1681 cttcattctt ccagcggttt ttattacggg attttttgct tggggttaca aacaatacgg
     1741 acaacttcca gaagtacaac cctttgttta cggaataaaa ccagcgatta ttgccattat
     1801 attgggcgct atttttcctt tggctaaaaa atctttaaaa tcaaccgaac ttatacttat
     1861 cggaataacc gttttagcgg gatccttact ccacttcagc gaaatctact tgatgtttgg
     1921 tgctggattt ttcgctttgc tattggccta tttgagagac aaggaacaaa aaaacttaag
     1981 tagttttcta cccttgactt ttataccaat aagcaataca actctaattt cggtaaccaa
     2041 tgctaatctg ttttggattt ttctgaaaat tggtgctatt ctttacggaa gcggttatgt
     2101 tttatttgcc ttcttagaca ccgaattagt tgcaacagga cttttaacac gacaccaact
     2161 gatagacgct attgcggttg ggcagttcac acccggccca gttttttcgt cagtaacatt
     2221 tattggctat caaattaatg gtttgacggg agccatcgtt tcaacaattg ccatttttct
     2281 accttctttt gtatttgttg ccttgctcaa ccccattgta aagaaaatgc gaaattctaa
     2341 actattctca gcatttttgg atgcagtaaa tgtagcttcg gtagccatta ttctagctat
     2401 ttgtttcgat atgggtaaag aaaccataac agattggcga accattagca tcgcgatttt
     2461 aagcattgca acagcctttg gataccagaa aataaatagt gctgttattg ttttgggcgg
     2521 ttcacttatg ggttatttat tgactctttt ataaccaaac acgataaaga aatctacaaa
     2581 gtagccttca aaacctcccc aattttcaaa gcacatttct ccccatcaat ggctgccgag
     2641 ataattccgc cagcataacc ggctccttct ccgcaaggat acaagccttt tatttgaagg
     2701 tgttccaatg tttctgcatc tctgggaatg cgcactggcg aagaagttct ggattcagga
     2761 gcgtgaagta cggcttcgtt ggtcaaaaaa ccacgcattg attttccgaa ttcaacaaaa
     2821 ccttctctta atatttgggt aagaaatcca gggaaaactt cgcccatttc taccgaagtg
     2881 gttccgggaa cataggaagt ttttggaata ttagaagata ctttcttttg ggtaaaatcg
     2941 accatttttt gggctggtac tttttgggtt tctccagcca aatgccaagc tttctgttcg
     3001 atgctttttt ggaattccat tccagccaaa gcaccaaatt ttgcaaatgg cttgaaatct
     3061 tcgagtttca attcaaccac aattccagaa ttggcggtag cttgatctct tttggatggc
     3121 gaccaaccgt ttgtaacgac ttcgccagga ctggtagcgc aaggtgcaat cacaccacct
     3181 ggacacatac aaaaagaata cattcccctt cctcctactt gcttcacaat agaataaggc
     3241 gatggcggca aatgctgtcc tcgataatcg caactgtatt ggatgctatc aattaacgac
     3301 tgcggatgtt cggctcgaac gcccaaggca aaagctttag cctctataaa aatttgtttt
     3361 ctgtccaata attcaaaaat atcacgagca gaatgtcctg ttgccaaaat tattttgttg
     3421 gcaagaatcg tgtcgccgtt tttggtcacg atgccttgca cttcattatt tttaatgaga
     3481 atatcggtca atcgagtttc gaaaagaacc attcctccca tttcgatgat tttctcccgc
     3541 atatcttgga tgatttgcgg taatttattg gttccaatgt gtggatgggc atcgaccaaa
     3601 atatcaggtg aagccccgta agcgacaaac aattccaaaa ttcgagtaac atcgccacgt
     3661 tttttggaac gggtatataa ttttccgtcc gaataggttc ctgctccacc ttcgccaaaa
     3721 caataattgg aatcttcatc tacaatatga tctctattga tggctttcaa atcgcgacga
     3781 cgacctcgaa catcttttcc acgctcaatt actatcggtt tcaaacctaa ctctatcaat
     3841 tgcaaagcgg caaacaatcc cgcaggacca gctcctacaa caattacttc tagtgcatta
     3901 gcaacattct tatattccgg taactcaatt ttagcttctt gaatagtttc gccaactaaa
     3961 taaatagtga ctttcaaatt gaatttcacc gctttttggc gagcatcaat cgaacgtttt
     4021 aaaatggaaa tatgttggat ttctttaaca tccacccgaa ttaatttggc tatgtgttgt
     4081 ttgagcaaca attcgtttgt agctgtttct ggcgatgttt gaaagagaag ttcttgtggc
     4141 attgtaaatt aatagttatt gaatagcaat aacgaaatgc aaaaatacta ctttgatgtg
     4201 aactttactt attgaattta aatccatttt caatttgtat ttttacagtc taaaaacatc
     4261 caaaaatgtc cagacaaata aaactaatat gggattttcg tggcccaact tcggcaaaaa
     4321 ctgctgaaca tcacgaaatt catttgaagg aatatataat tgccgagaaa ttatcgccaa
     4381 atattaccgg ttttcaaatc ataaatgaaa tgtatgcaac agcttttatg gtcgttactg
     4441 atgaaacgat gattcaagta cgcgatgcct tgaaaccaca tcgtggagag gtttttgaag
     4501 gttaaaatcc aataaaaaaa ttccaaattc caacttagaa gattggtatt tggaatttaa
     4561 aaaattggaa tttatttcta attcgctact tcgctcataa atttgatacg catcagacgt
     4621 aattcgtcaa tatcataatc gccgtcaaat tcttttagag cttcctctat attatcggaa
     4681 tgggattcca taaaataatc gtgaatttct tcttgttgat catcgtccag catttcatca
     4741 atccaatact tgatatttaa tttggttcct gaattgacta tttgctccat ttcagtaatc
     4801 aaggcttcca ttgtcaaccc tttggcagaa gcaatatcat tgagcggtaa ttttctatca
     4861 atattttgaa taatatataa tttattgatc gaattggctc ctgtggattt tacaaccaaa
     4921 tcttcgggac gaataatatc attgtcatcc acatattgac tgattaaagc aatgaattcc
     4981 ttgccatatt tcttagcttt tccttcacca actccgtgtg tgttgatcaa ttcatccatt
     5041 gaaatgggat atttcaaagc catatcttct agtgaagggt cttgaaaaac cacaaatgga
     5101 ggaactccta gttttttggc tactttttta cgtaagtcac gcaacattcc cattaaaact
     5161 tcatcggcaa ctccagaacc ttttgcagca gttacgatag cttcatcctc agattcgttg
     5221 tattcatgat cttcagacat catgaaggac tcaggttttt tgataaaatc caatcctttt
     5281 tgagtgattt ttatcactcc gtatgtctca atatctttag ataataatcc atcaaccaaa
     5341 acttgacgca ataaggccat ccagtatttt tcgtcgtgat ctgtcccaga gccaaaaaag
     5401 gattgaacat cagttctatg cgcttttata atagcattta cgcgtccgat gagggtaaaa
     5461 acaacttctt tagatttata aatgtgtttg gtatcacgaa ctacttcgag caatttgacc
     5521 acttgatctt tagcttcgac cttcactttt ggattgcgaa cattgtcgtc catatcgcca
     5581 ccttctccag tttcagtatc aaactcttca ccaaaataat gaagtaaaaa ctttcttcga
     5641 gacatagaag tttcggcata agctacaact tcctgcaata aagcaaaacc aatttcttgt
     5701 tcggcaacag gttttcctga caagaatttt tctaatttct cgacatcttt ataagagtaa
     5761 tatgccaagc aatgcccttc gccaccatca cgtccagcac gacctgtttc ttgataataa
     5821 ctttctaatg attttggaat atcgtggtga attacaaaac gcacatcggg tttgtcaatt
     5881 cccatcccga aagcgatggt tgccaccaca acatctacat cttccataag aaacatatct
     5941 tgatgtttgg ctcttgtttt agcatctaat ccagcgtgat aaggaacggc acttattcca
     6001 tttacttgca aaacttcggc aatagcttct acttttttgc gacttaagca ataaataatt
     6061 cctgatttcc ctttgtgtaa tttgataaac cgaatgatat ccgcttcaat atttttggtt
     6121 ttggtacgaa cttcgtaata taaattaggt ctattgaaag aagccttata agttgttgca
     6181 tctaccgtat ctaaattttt caagatatct tcttgaactt ttggagttgc tgttgcagta
     6241 agacctataa taggaacgtt acccaattgc ttgataatat gttttagatt tcggtattca
     6301 ggtctaaaat catgtcccca ttcagagatg caatgtgcct cgtcaattgc aacaaatgaa
     6361 attggcacat tttgtaaaaa agctacatat tcttcctttg tcaaggattc gggagctaca
     6421 tacaaaagct tggtcaatcc tgaagtaata tcggttttga cttgagcaat ttcggtttta
     6481 gtgagtgagg aatttaatac atgagcaacg ccattttctg aagataaact tctaatagca
     6541 tctacttgat tcttcattaa ggcaattaaa ggagaaacaa cgattgctgt tccttcttga
     6601 actaaagcag gcaattgata acaaagcgat ttgcctccgc ccgtaggcat aatcacgaaa
     6661 gtgttttctt tattgagaat acttttgata acttgctcct gtaaaccttt gaattggctg
     6721 aagccaaaat acttctttaa ttctctgtgg atgtcaattt cgtttgaatt cattctttat
     6781 taatggtatt ttatctaaat ttgcgtttca taaagatact aatttctttc atacatacaa
     6841 attttataaa attctatttt gaatacaaaa gaaaatatat tgattttggc aaaaaaaact
     6901 attctctctg agagcgaatc tattgctaaa ctcattgatt ttattgactt taacttcgta
     6961 gaagccacac aaattctttt tcaatcaaaa ggaagattga ttgtaacagg cattggaaaa
     7021 agtgccatta ttgcacaaaa aatggtggca acatttaatt ctacaggaac accatcgctg
     7081 tttttgcatg cttccgaggc cattcatggc gatttgggaa tggttcagcc tgacgatatt
     7141 gtgctttgta tttcaaaaag cggtaacagt cctgaaatta aaattttagt tccactccta
     7201 aaacgttttg gaaatacgct aattgctatg accggaaata tcagttcgtt tttggcaaaa
     7261 gagtctcaat ttgttctaaa caccactgtt gatgcggaat cttgtcctca taatttggcc
     7321 ccaacaaata gtactaccgc acaactcgtt atgggtgatg ctcttgctat gtgtttgatg
     7381 aatttaagaa atttcagtcg tgaggatttt gcaaaatacc atcctggcgg ttcattgggt
     7441 aaaaaattat tacttcaagt gaaggatatg cttgaaaact ccttgaaacc aatggtaacg
     7501 cccgatgcac ctattaaaaa agtaatttta gaaatttctg aaaaaagact tggcgcaaca
     7561 gctgttgtcg aaaatgacaa agtaattgga gttattactg atggtgatat tagaaggatg
     7621 ctcaatgaaa atgatacttt tacgcatttg actgccaaag atataatgac aaaaacgcca
     7681 aaaatgattc aatcgaccac aatggtggtg caagcactga atattcttga agatttttcg
     7741 atcactcaat taatagttgt tgatcacgaa caatacaaag gaattattca tttgcatgat
     7801 attttaaaag aaggaatagt ataatggcga ataaacaact taaagaaatg tcttttttag
     7861 accatctcga ggaattaaga tggttattag ttcgaagtac gatagccatt atcattatgg
     7921 cattctttac ttattttgta agcgattatt tgtttgacac catcatattt ggaccaacaa
     7981 gaccttcatt ttacacctat cgctggtttt gtgaattatc acattattta aattttggcg
     8041 aaagtatttg tattgaagaa ttgcctttta tcattcagaa tactgaaatg gaaggacaag
     8101 taaatatgtt tgtttggatg tgtctattgg ctggttttat acttgctttt ccttatatat
     8161 tattccaaat ttggggcttt atcagtcccg cattgtatgc gaatgaaaag aaaaacgcta
     8221 aatttttcat ctttatttcc tctttattgt tctttttagg agtccttttt ggctatttca
     8281 ttattattcc aatgtcggtc aattttgttg ccacctttac tgtgagcgaa atggttaaaa
     8341 atcaatttac cttagattcc tatatcggaa tgtttaaaac atcggttata gcgggaggtt
     8401 tattttttga attacccata attatttatt tcttgacaaa actaggatta gtaactcccg
     8461 aatttctgag gaaatatcga aaatatgcta tagtcatagt attaatagtt gccgcaattg
     8521 ttactcctcc agatgttgta agccaaacaa tagttgctat tccgatgttg ataatttttg
     8581 aattaagtgt aataatttct tctattgttt ataaacgaaa aataaaaaat gagcaactta
     8641 gttaaagaat tcaacgacta tcgttctaaa atgaacgaaa aattattggc cgataataat
     8701 aaaattgtaa agcgaatttt caatcttgac accaatgcct atgcagatgg agcgcttgat
     8761 gttagaacca aagaattatt aggtttagtc gcttcgactg ttttgagatg cgatgattgc
     8821 gtaaaatacc acttagaaaa ttgctataaa gaaggaattt caaaagaaga aatgatggaa
     8881 gcaatgggca tcgccacatt agttggcgga actattgtta ttccacacct gagaagagct
     8941 tatgaatttt gggaagtgtt ggaaaataat taaacgattt aacaatttgt caatgtatca
     9001 attaaaaata agattgctac attgtcacat tatataattt tcacattaaa aatatgaagt
     9061 taagagccga aaatctagta aagacatata aagggcgaag cgttgtaaaa ggcgtttcgg
     9121 tagaagtaaa ccaaggcgaa attataggac ttctggggcc caatggtgcc ggaaaaacaa
     9181 cttcatttta tatgattgtt ggattagtaa aacccaatat gggcaacatc tatcttgacg
     9241 atctaaacat cactgatttc ccgatgtaca aaagagcgca acaaggtatt gggtatttgg
     9301 cacaagaagc ttctgttttt agaaaactaa gtgttgaaga taatattttg agcgtgttgc
     9361 aactcacaaa cctttccaaa gaagctcaag aagagaaaat ggaaagtttg atagaagagt
     9421 ttagtttggg gcacatacgt accaacagag gcgatttact ttcgggtggc gaacgtcgtc
     9481 gtactgaaat tgctcgttgt ttggctaccg accctaaatt tattcttctt gatgagcctt
     9541 tcgcaggagt tgacccagtt gctgtagaag acattcaacg aattgtagcg caactaaaaa
     9601 ataaaaacat tggaatcctg attactgacc ataatgtgca agaaactttg gccattacag
     9661 acaaaaccta tcttatgttt gaaggcggaa tcctaaaagc tggagtccca gaagaattgg
     9721 tagaagacga aatggtacgt cgtgtatatt taggtcaaaa tttcgagttg agaaaaaaga
     9781 aaatagagtt ttaatgatag tgttccgttt gaaaaaaaag ataattataa aaaactatga
     9841 ataactctaa taattggaga gacaacgatc ccgataaaca aacagcggaa aattggcgca
     9901 aagaccctaa gaattggatt tggaatatct tttattacaa caaagaagat caacgtcttt
     9961 ttgtttcaaa aaaaatagaa tggatgggaa caacacttaa tttcgcaaac ccaaaatcgg
    10021 ttttggtttt agttgcgatg ggttcttttt tggggatggt tctctatttt atcttaaaaa
    10081 actaaaaact actcgattgt aataaaccgc aactgctcta catcaccgtt aaaaatattg
    10141 acatctacat tgccttttat tcctgaaact gaagcttttt cggtgaattg ccaaaaaagc
    10201 caatcctctt ctattttttc tcgataaaag ttataattgg caatccaaaa aaggtaatct
    10261 gaaaagtctt tcttcaaaaa atcatcataa tatttctcgc ctgtataaat aataggtttc
    10321 actttataat gcgcctcaac agctttcaac caacgtctca atccaatttt caaactatct
    10381 aaagattgat ctttaggcaa tttttcaata tctaaaacag gaggcaaatc accttttctc
    10441 aaacgaactg tttttataaa aagtttagct tgctctaatg aattttcgtt tggacgataa
    10501 taatgatagg ctccacgaat gagtttttgc tttttgactg ctttccaatt ctgctcaaat
    10561 tgctggtctt cgccatcatt tccagccgtg gcacgaacaa gaacaaacga aactggatat
    10621 tcttcctcta cattttgaac caaatcccaa tcaatttctc cttgatattc tgaaacgtct
    10681 ataccaatgg ctttgccttt atgattatct aaaacctgat aattccgaat atccgaaatt
    10741 cgcttggatt gctcttgttc gtattttaat ttataggatt taaaccccaa ataatacaaa
    10801 aaaccatttc gataatgata ccctagaccg ataaatagca aaataaaaaa tccaattata
    10861 gaaaaacgca acagttttcc cgagaaaaag gaaccttttt ttctaggttt tcgtctaatt
    10921 acatgtttgc ttcgtgtagt tttccgcctc attgaaaaac tactttttca aaaatttgtt
    10981 attgataaca gacaacaaaa cataagtaat aatgactaaa ggaatggcta ggtattgaaa
    11041 gaaaatcaac agcaaaacac acaaactcaa aaagacaatt tgaagtgcat ttttcttaaa
    11101 agtaaaatct ttaattttca aagaaaacaa aggaatttcg gcatttagaa tgaaagcgct
    11161 aaataaagta atccctaaaa gaacccaata attggtcaga atttcaagca aaactaatga
    11221 atctgaatat tttaaaacca atggcaaact caaaataaac aaagcatttg ctggagttgg
    11281 caagccaata aaagaatcgg tttgacgtgt gtcaatatta aaattcgcca aacgataaca
    11341 agaaccaagt gcgataataa accctaaaaa agaaaaataa tgattgggat tggaactatt
    11401 ttccatcatt gaaaacatca caaaaccagg aacaactccg cttgtaacca tatcagctaa
    11461 ggaatctaat tgcaaaccaa gaggacctgc aaccttaaac aatcgggcaa aaaaaccatc
    11521 aaaaaaatct agaaaaattc cgagactaac aaaaaagaaa gccatttcaa aattcaattc
    11581 ggctacaaaa aacacggcaa tacaaccgca aaaaagatta agcaacgtga tgatatttgg
    11641 gatgtgtttt tttattttca tggttataaa aatttagatt gtcaaagttc acaaatttag
    11701 tataataagt caatgagaag tgcgtttttt agaagagatt taccttccga agcttttatg
    11761 tttggaatgg gttgttaaaa aagagattag ctcagtaaaa aattatattt ttggaaaaaa
    11821 ttaaacagcc tttgggttgc aaacgaattc atgattaaaa aaaatcaatt cttcctactc
    11881 cttttgcttt gcgttagttc gtatgctcaa accgttagaa agtattcaaa cgagtttatg
    11941 aatattggtg tagatgcggc agctttagga atgtcaaacg ctgtgacttc aagcaccaac
    12001 gatgttaatt cgggctattg gaatcccgca ggattggtaa atctcgaaga ccatcaactg
    12061 tctttgatgc acgccaatta ttttgccaat attgccaaat acgattatat cgcctacgcc
    12121 aatactattg atgacgaaag tgcttgggga atttctttaa tccgttttgg tgttgacgac
    12181 atcatgaaca caaccgaatt gattgacagc caaggaaata ttgactacaa tcgcatcagt
    12241 cttttttcta cagccgatta tggcgttact ttttcatacg ctcgaaaact gcaagttcct
    12301 ggttttcaat ttggtgttaa cgccaaaatt attcggagaa tcataggcga ttttgcaagt
    12361 tcatggggtt ttgggtttga tgcaggtttg caattcgaaa aaaatgattg gaaatttgga
    12421 ttgatgcttc gcgacatcac aacaacctat aatgtatgga gcattgacga agccgaatat
    12481 aaaaagattg cagatgctat tccaggacaa aatcaggaat tgcccgaaag cacagagatt
    12541 acagctccaa aagcacaatt aggattatcc aaaaaattta ttgtacgata tgactatagc
    12601 attttggcag cagccaatct caatatgcag tttgccaaaa caaatgatat tctttcgact
    12661 gatttcgtga gtgtagatcc tgctttgggt tttgaatttg gatacactga cttagttttc
    12721 ttgagagctg gcgtggggaa ttttcaaaac attcaacaaa ttgatactac cgaaaaagta
    12781 ggttttcaac ccaatattgg tttgggtttt aaatacaaag gcatccaaat tgattatgct
    12841 ttgaccaatt taggaaatca aagtgctgct ttatattcga atatattttc ggtaaaagta
    12901 gatttggggc tttttagaaa ttaattctta aataaattca aaaatgaatt cattgtcatt
    12961 aaaaaaaata gtgctaatcc tttttttaat tggaacgctg agtggttttg gacaaaatat
    13021 aatactgtca aaaagtgctc gaacaagcgt tattacttgt gacactggca atgaatccta
    13081 ttcacttttt ggacacacag ccatcagaat ttccgaccca gaaaataatt tagatgtggt
    13141 ttataattac ggtgcttttg atttttcgac acctaatttt gttgtaaaat ttgccaaagg
    13201 cgatttgcaa tactttgcta ttgccaatac tttttcggat tttatgaacc aatacactta
    13261 tgagcaaaga agtgtttttg agcaagaatt attgattcca ttggcataca aacaaaaatt
    13321 gtttgataat ttaacaactg tattatctac aagcgaaagt tattatacct ataaatttat
    13381 agataaaaac tgtacttcga tggtggtgga tatgctgaac aaaacattag gtgtaaaaat
    13441 aatagataag aaattcgaca ctgacaagac ctacagaagt attttatttc cttattttga
    13501 taatttcttt tatgaaaaac taggaaccag catcattttt ggaacaaaag tagataacta
    13561 tagcgatcat atctttttgc catttgaact taaaaaaagt ttgcaagaaa ctcagttcaa
    13621 aaacaaacgt ctttgtgcgc aaagcaaaac tttattagaa tacaaaaaac aagcgcctaa
    13681 ttcttggtgg aacaatgtgt attcgtattt gttgtttctt gttttcatcc ttattttgaa
    13741 caaaaaaatt atcgataatt tttatcttct aataatggga ttattgggga ttttctttat
    13801 tgtaatggga ttttattctt ttcaccaaga attggcaatg aattacaatg tgttgttgtt
    13861 caatcctgcc ttgctcgttt tgctttactt tatagtcaag aaaaacaaaa aatggattgt
    13921 aaatcttgct gtcctaaacg gcatttttct tggagtttac ctgataatga tgattaataa
    13981 agcccatttt ttagtcgtta ttcctttggt actaactact ctaattatag tatcaaaaat
    14041 agcttataaa aacagtaaac gcattccagt agttatttga ttactgacct cggtaaaaca
    14101 atacggtgct gaatgtttta ataactatgt ccaaatccaa gaaaatacta cggtgcttaa
    14161 tgtaatataa atcatattgc agttttatta aactatcttc catcgattcg ccataggaat
    14221 aattaacttg tgcccaacct gttagtcctg gttttataac atgtcgagtt tcatagaaag
    14281 gcattatttc ggcaatttcc ttaacaaaga aaggtctttc aggtcgtggt cctataacgg
    14341 ccatttcacc tttcaaaata ttgacaaatt gtggaaattc atcgattctt gttttccgca
    14401 taaatttccc aaaaggagta actcgagtat cattcgaacc tgagaaaacg gctcctcctt
    14461 tttcggcatt atttatcatt gtcctgaact tgagtatttg aaatatttca ccatttttgc
    14521 ccactcgttc ttgtgtataa aataacttac ctcgatttcc tatggcattt cctattgcaa
    14581 taaaaggaat taatagagtg ccaataatta gtccaattac agaaattaat atttccatta
    14641 ttcgaacatt taacaaatac aattggttgt tattcgttct attaaatggg aagaatttat
    14701 aaaaatctcg agtaatatat tgaagaggaa ttcgatgtgt tttatcttca tagacttgag
    14761 tatattcgcg aattgttgtt cctgattcta acaaatgaag caactgcata tacaaatttg
    14821 aagtaatatc atctgtattt tgtgaggcta caacaatttc aaataatcta ttttcattca
    14881 aaaacaaaac caaatcatca atttcaacgt gttgaacata atggtcctcg aattcatcct
    14941 tttttccggc ataggagtag tttacataag ctacaattct gtaatgagga tcagagtttt
    15001 ctaagtcaga aattaatcct tcaacttgat ctctatgaca aaccaacaat acattctgca
    15061 caaatcgatt ggaagccaac agtttaacat aaaacattct ccaaaaaatc agagtaaaaa
    15121 gtaccgtaag ataaaacact actatttcaa atcgattgga aggtaattct gctgaaaaaa
    15181 caggggtcaa taaataaaac aaaacggcag ttgaagcagt gagaattgta cttcgcatta
    15241 cctgaaattg gttgcttgat ccaacattaa atttaggtgg aggttatact ttcttaggaa
    15301 aagacagctt cggtcacgtt aatttagg
//
LOCUS       DAOSNN010000029        14928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_4029_length_14928_cov_6.498958, whole genome shotgun sequence.
ACCESSION   DAOSNN010000029 DAOSNN010000000
VERSION     DAOSNN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4029_length_14928_cov_6.498958"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="PLQ70_02795"
     CDS             <1..168
                     /locus_tag="PLQ70_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013186562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HQE33570.1"
                     /translation="IIPLKLLEMGCLPGNTVQLLQIAPFGDPLYLNVNGSHLAIRIET
                     AREIEVDCITK"
     gene            175..2277
                     /gene="feoB"
                     /locus_tag="PLQ70_02800"
     CDS             175..2277
                     /gene="feoB"
                     /locus_tag="PLQ70_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQE33571.1"
                     /translation="MTKQNINVALIGNPNTGKTSVFNQLTGLNQQVGNYPGITVEKKV
                     GFCKLAHNINANVIDLPGTYSLNASSADESVVIELLLNKNDKLFPDVIVVVSEVENLK
                     RNLLLFTQIKDLEIPTILVINMIDRMKLKGISLDIPFLEEHLKTKIVLVSSRKNIGIE
                     ELKTAITTYKTITTEACLNASSIDDDYFNTLRKTFPNQSLYKLWLVITQDVNFADLNR
                     NTLENESFTKSKSELKKLQQKETIKRYQFINDTLKTGQNIDSSIATDFRSKLDRILTH
                     KVWGYLIFFVVLFGIFQSIFEWSKIPMDFIDSSFASLSTAASENMPPGAFTNLIAQGI
                     IPGIGGILMFIPQITFLFLFISILEESGYMSRVVFLMDKIMRRFGLSGKSVVPLISGT
                     ACAIPAIMATRNIENWKERLITILVTPLTTCSARLPVYAIIIGLVIPDKYLLGILNLQ
                     GLTLMLLYLIGFGMAILSAYILNKVLQLNCKTFFVVEMPNYKLPMFKNVMITVVEKTK
                     AFVFGAGKIILAISVVLWFLASNGPSKDFRNAETIITSKTENATLSKVEIENKIAAYK
                     LEKSYIGLMGKTIEPAISPLGYDWKIGVAIISSFAAREVFVGTLATIYSVGGSDNDNT
                     IRNRMKEEINPETDQKIFNFASGISLLLFYAFAMQCISTLAVTKKETNSWKWPLGQLV
                     FMSSFAYIVALIAYQLLK"
     gene            2283..2405
                     /locus_tag="PLQ70_02805"
     CDS             2283..2405
                     /locus_tag="PLQ70_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HQE33572.1"
                     /translation="MIQDILTYLTLAFAIVFLVKKFFFKKKKTNKNCGEDCNCQ"
     gene            complement(2399..3058)
                     /locus_tag="PLQ70_02810"
     CDS             complement(2399..3058)
                     /locus_tag="PLQ70_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HQE33573.1"
                     /translation="MTLSEENYLKAIYHLTTVTDADVSTNAIAEMMETKASSVTDMLK
                     KLAEKDLVNYKKYQGVSLTNKGLLSAKMIVRKHRLWEVFLVDKLNFAWDEVHDIAEQL
                     EHIKSEQLINKLDDFLGNPTEDPHGDPIPDAQGRMAKMDKQLLSDLAINQIGICVGVK
                     DSSAEFLKYLDKQAIALGSKIEILAKESFDLSTKMRVNETTELTISNKIANNLFVKVD
                     Y"
     gene            complement(3130..5001)
                     /locus_tag="PLQ70_02815"
     CDS             complement(3130..5001)
                     /locus_tag="PLQ70_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HQE33574.1"
                     /translation="MSKSLEEVNQSVQTQNKTSIFRKILAFFGPAYLVSVGYMDPGNW
                     ATDIAGGSQFGYSLLWVLLMSNMMALLLQSLSARLGIVTQRDLAQASRETYSKPINYI
                     LYFLAEIAIAACDLAEVLGMAIGINLLFDIPLIEGVLITVLDTFLLLFLINKGIRKME
                     AFIIVLVAIIGFSFVFEMIFAQPELDKVVYGLIPSIPTETALYIAIGIIGATVMPHNL
                     YLHSSLVQTRKFDRTPDGIKQALKYNFIDSTIALNLAFFVNAAILILAAATFYKNGYF
                     EVAEIQDAHQFLQPLLGTKWAPILFAVALIAAGQSSTITGTLAGQIVMEGYLNFRMQP
                     WVRRIITRLIAIVPAVVVILIYGESVTGKLLILSQVILSLQLGFAIIPLIHFVSDKTK
                     MKGFHISKITQVAAWIIATIIVSLNAKLVFNEIVGWLETSENPIVLWLTVVPLAFGFL
                     ILLLYIVFKPFITKAKQEFQNHSPHNLNLQFSKIGTYNTKKIAIAVDFSSADTIAINS
                     AFELGGIEAKYTLIHVVETVGAMVYGKNIEDHETLIDAKLLAEYKAMLSHKGFTVKTK
                     LGYGKPNKVIPEIINKGNFDVLVMGTHGHTGFKDLIFGTTVDKLRHKISIPLFIVKN"
     gene            5094..7175
                     /locus_tag="PLQ70_02820"
     CDS             5094..7175
                     /locus_tag="PLQ70_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE33575.1"
                     /translation="MKYIFLLISFFSINLLFSQNTIPKTENDSIEKKDNQNELNEVVV
                     SGTLKPIRRLESAVPVEVYSPVFFKKNPTASIYEALQNVNGVRPQLNCGVCNTGDIHI
                     NGLEGPYTSVMIDGMPIVSSLSTVYGLSGIPNSLVERIEIVKGPASSLYGSEAVGGLI
                     NIITKNPNNAPLFSADVFTTSWLETNADIGGKFNIGKKATSILGLNYYNYNQTIDNDN
                     DSFTDVTAQNRISVFNKLNFSRNQNRLFTIAVRGMYEDRWGGDVRWEKKYRGGNEIYG
                     ESIYTKRGELIGSYQLPTTEKLMLSFSGTMHFQDSRYGTTSYIANQKIGFAQLTWDKK
                     IQNNDLLAGIASRYTYYDDNTTATAILTENNPEKTWLPGIFLQDEITFSEKHKVLLGI
                     RYDYNSIHGNIFTPRFAYKVKFNNNNILRLNAGTGFRVVNLFTEDHAALTGSREVVIA
                     ENLDPEKSINANLNYIKKIYLDNGTFLGIETTAFYTRFSNKIVSDYEIDPNKIIYDNI
                     DGYALSQGISCNTDINFTNGLKFILGATYMDVSNVENGIKTRPLLTENFTGTWTVSYK
                     IDKIKTIIDYTGNVYSPMKLPLLSETDPRNPNSPWYSLQNIQFTYSGWKNFELYAGIK
                     NLLNFTPKRNNPFLISRTEDPFDKNVQYDSNGKVLVTPDNPYGLTFDTTYVYGPNQGI
                     RGFFGLRYNFY"
     gene            7256..7720
                     /locus_tag="PLQ70_02825"
     CDS             7256..7720
                     /locus_tag="PLQ70_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HQE33576.1"
                     /translation="MKKSLYILLIFFWTMPSGFAQLKTHTFEEAEKLSKENPKPFVIF
                     INTSWCNYCKMMENSTFKNPEIISLLNSDFYFISLDAESKSDIHFNNHTFKFQPKGQN
                     TGIHELATQLATIDSQVMYPTLTVLQSDFSIVFQKHSFLKAKELIITLEKLK"
     gene            7829..8239
                     /locus_tag="PLQ70_02830"
     CDS             7829..8239
                     /locus_tag="PLQ70_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HQE33577.1"
                     /translation="MYPQEMVLPMQAELTSVGFQDLHNADAVNDAIKAEGTTLVVVNS
                     VCGCAARNARPGAAMSLEGAKKPDHLVTVFAGVDKEAVNAAREHMFPFPPSSPSIALF
                     KNGELVHMLERHHIEGRPAELIADNLKDAFNEYC"
     gene            8419..9786
                     /locus_tag="PLQ70_02835"
     CDS             8419..9786
                     /locus_tag="PLQ70_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HQE33578.1"
                     /translation="MQLYNTLSAEERTVMIDDAGKQRLTLSFYAYAQIQNPTQFRNDL
                     FLAWNPLEVLGRIYVAHEGINAQLSLPADNFYAFKDTVEAYDFMKGMRLNIAVEHDDH
                     SFLKLTIKVRDKIVADGLNDATFDVTNIGIHLKAKEFNEILENPDTIVVDFRNHYESE
                     IGYFKGAITPDVDTFRESLPIINEQLKDFKEDKNLVMYCTGGIRCEKASAYFKHQGFK
                     NVYQLEGGIINYAKQIKEENLESKFIGKNFVFDHRLGERITDDIVSQCHQCGAPCDVH
                     TNCLNEGCHLLFIQCESCKEKMHGCCSTECVTVIHLPEEEQKAIRRGVKNGNKIFKKG
                     KSDVLTFKNNQDPLANVPNLSELIKSKPLLKKEPKIKKQYLGKGTHFFPKTNIAQFLI
                     EENQINIGDSILIKGATTGEEMLIVEKMLVNDVESQKAVSGEMVTFKVPFRIRLSDKL
                     YKILN"
     gene            9855..11096
                     /locus_tag="PLQ70_02840"
     CDS             9855..11096
                     /locus_tag="PLQ70_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HQE33579.1"
                     /translation="MTTNNKIELMAPAGNFESLQAGLDNGADSVYFGVEQLNMRARAT
                     VNFTMDDLKEIARRCEEKNVRSYLTLNTIIYDHDLSVVKTLLNKAKEANITAVIASDQ
                     AVIAMARGIGMEVHISTQLNITNIETIKFYSLFADTMVLSRELSLRQVKNITEQIIKE
                     DIKGPNGKLVEIEIFGHGALCMAVSGKCYLSLHSDNSSANRGACKQNCRKKYTVIDQE
                     TGFEIEVDNEYLMSPKDLCTLDFLDQVIDSGIQVLKIEGRGRAPEYVATVVKTYREAI
                     DSYYEGSFSKEKIAVWMEALSTVYNRGFWSGYYLGQELGEWSDVSGSMATQKKVYVGK
                     GTHFFPKAEIGQFKIEAYDIQIGDKILVTGPSTGAQEMVIEEMFVNDVTAEKATKGDD
                     CTLKLPFRIRMSDKLYKIVEA"
     gene            11096..11326
                     /locus_tag="PLQ70_02845"
     CDS             11096..11326
                     /locus_tag="PLQ70_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HQE33580.1"
                     /translation="MVIVTLQRAKCIGCNYCVEMAPVQFQMSKKDGKSVLIKSVNAKG
                     FFTLKSPDHTITESCELAAKACPVKIISVKET"
     gene            11500..12855
                     /gene="ricT"
                     /locus_tag="PLQ70_02850"
     CDS             11500..12855
                     /gene="ricT"
                     /locus_tag="PLQ70_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HQE33581.1"
                     /translation="MACTSCSTSDGGAPKGCKNNGTCGTDSCNKLTVFDWLSNMSLPN
                     GEAPFDCVEVRFKNGRKEFFRNTEKLTLSIGDIVATEASPGHDIGIVTLTGELVRIQM
                     KKKGVNSSNTDVPKVYRKASQRDIDIWSIARDREEPMKVRARELAISQKLEMKISDIE
                     FQGDGSKATFYYTANDRVDFRLLIKDFAKEFSTRVEMKQVGLRQEAARLGGIGSCGRE
                     LCCSTWLTDFRSVNTSAARYQQLSLNPQKLAGQCGKLKCCLNYELDTYMDALKGFPDF
                     DTKLVTEKGDAICQKQDIFKGLMWFAYTNDFANWHVLKIDQVKEIVAENKLKNKVASL
                     EDFAIVIVQEPEKHFNNAMGQESLTRFDQPKKKKKPNKKRKNGENLIVANNNNVNANT
                     NSNTTSKPNNNNKPNNNRPNNNNRPNNNKPKPNNNNNTNEKKPVVARKPIIITKNENK
                     E"
     gene            12839..13318
                     /locus_tag="PLQ70_02855"
     CDS             12839..13318
                     /locus_tag="PLQ70_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HQE33582.1"
                     /translation="MRIRNSAILLLVVILFFSCDKKRVFDEYKSVGTAWHKDSIVSFN
                     LPELDSTKRYNLFVTLRDNNNYPFNNLFLIVGLEMPNGFTKVDTLEYEMANPDGSLMG
                     EGFSDIKENKLFYKEKVRFRGKYKVNIKQAVRENGKVPGVTALEGITEVGFRIEKID"
     gene            13325..>14928
                     /locus_tag="PLQ70_02860"
     CDS             13325..>14928
                     /locus_tag="PLQ70_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQE33583.1"
                     /translation="MATQKKNIQTKNIEKDITYYKKKFWKVFFYGLGGLLLFFLFASW
                     GLFGSMPSFEDLENPDSNLATEIISSDGVILGKYFEKNRSQLKYSDLPKNLVDALVAT
                     EDERFYEHSGIDGRGTLRAIASLGTSGGASTLTQQLAKQLFHGEGSKFLPFRIVQKIK
                     EWIIAIRLERQYTKNEIIAMYCNVYDFGNYSVGVSSAAQTYFSKAPKDLTIDESAILV
                     GMFKNSGLYNPVKNPQGVKNRRNVVLSQMEKGGIITQGQKEKLQALPITLHFKLESHR
                     EGTATYFREYLREYMKKWMETNKKADGSDYNIYKDGLKIYTTIDSRMQLHAEEAVAKH
                     MENLQEQFFEEAKTNKNAPFVNITEAETQRILKQAMKASYRWTVMSEMEKSEEDIIAS
                     FSKKTKMKVFTWKGERDTIMTPLDSIRYYKHFLQSGLMAMEPQTGNIKAWVGGINYKY
                     FQYDHVGQGARQVGSTFKPFVYATAIEQLGMSPCDTILDGPFMIRKGRHHVTEDWEPR
                     NSNNQYRGMVTLKQGLANSINTISAK"
BASE COUNT         5180 a         2706 c         2629 g         4413 t
ORIGIN      
        1 attattccgt taaaattatt agaaatgggt tgtttgcctg gaaacacggt tcaattactt
       61 caaattgctc cttttggcga tcctttgtat ttaaatgtca atggctcaca tcttgccatt
      121 cgcatcgaaa ctgctcgtga aattgaagta gattgtatta caaaataact ttaaatgacc
      181 aagcaaaata tcaatgtcgc tttaattgga aacccaaata ctggaaaaac ttcggttttt
      241 aaccaactta ctggcctgaa tcaacaagta ggaaattatc ctggaattac ggtcgaaaag
      301 aaagttggtt tttgcaaatt agctcataat atcaatgcaa atgtaatcga tttgccagga
      361 acctatagtc ttaatgccag ctcagctgac gaaagtgtgg tgattgaact tttgctcaac
      421 aaaaacgaca aactctttcc cgatgtgatt gtggtagttt cggaagtaga gaatttgaaa
      481 cgaaatttac tgctttttac ccaaataaaa gaccttgaga ttccaactat tctagtcatt
      541 aatatgatag atcgtatgaa actcaaagga atttcgctag acattccatt tcttgaagaa
      601 catctaaaaa ccaaaattgt attagtaagt tctcgaaaaa atattggaat tgaagaacta
      661 aaaactgcaa tcacaaccta caaaaccatt acaacagaag cttgtttgaa tgcttcttct
      721 attgatgatg actatttcaa tacacttcga aaaacgtttc caaatcagtc tttgtacaaa
      781 ctttggctag tgattacaca agatgtaaat tttgcagatt taaacagaaa tacacttgaa
      841 aacgaatcgt ttacaaaaag caaatccgaa ctcaaaaaat tacagcaaaa agaaaccatc
      901 aagcgttacc aatttataaa tgacacttta aaaacaggtc aaaatataga ttcttcaata
      961 gccaccgatt tcagaagcaa actagatcgc attctcaccc ataaagtttg gggttatctg
     1021 atattctttg tcgttttatt cggaattttt caatccatct tcgaatggtc aaaaattcca
     1081 atggatttca ttgatagtag ttttgccagt ttaagcactg cagcaagcga aaatatgcct
     1141 ccaggagctt ttactaattt aattgctcaa ggaattatcc ctggaattgg cggaattttg
     1201 atgtttattc cacaaattac ctttctgttt ttgttcatat ccatcctcga agaaagtggc
     1261 tatatgagcc gagtagtgtt tttgatggac aagattatgc gccgatttgg actttcaggc
     1321 aaaagtgttg ttcccttaat ttcaggaact gcctgtgcca ttccagccat tatggcaacc
     1381 cgaaatatcg aaaactggaa agaacgatta atcacaattt tagtaacacc actaactact
     1441 tgttcggcac gattacctgt ttatgctata ataattggat tggttattcc cgataaatat
     1501 ctattaggca tattgaattt acaaggattg accttaatgt tgttatatct tattggtttt
     1561 ggaatggcaa ttctttcggc ttatattctt aataaagtac tacaactcaa ttgcaaaact
     1621 ttttttgtgg tcgaaatgcc caattacaaa ttgcccatgt tcaaaaacgt gatgataacc
     1681 gtggtcgaaa aaaccaaagc ttttgtattt ggagcaggga aaattatctt ggcaatatca
     1741 gtagttttat ggtttttggc ttccaatggg ccaagtaaag atttcagaaa tgccgaaaca
     1801 attattactt caaaaaccga aaatgcaacc ctttctaaag ttgaaataga aaacaaaatt
     1861 gccgcttaca aactcgaaaa atcatacatt ggattaatgg gaaaaaccat cgaaccagct
     1921 atttcgcctt tgggttacga ttggaaaatt ggtgttgcta tcattagttc atttgctgca
     1981 agagaagttt ttgtgggaac attagccacg atttatagtg ttggtggtag cgacaatgac
     2041 aacacgattc gaaaccgaat gaaagaagaa attaatcctg aaacggatca gaaaatattt
     2101 aattttgcct caggaatttc gttattattg ttttatgcct ttgcgatgca atgtattagt
     2161 actttagccg ttacaaaaaa agaaaccaac agttggaaat ggcctttagg acaattggtt
     2221 tttatgagta gttttgccta tattgtagcc ttaattgctt atcaattatt aaaataatcg
     2281 caatgattca agatatttta acatacctca ccttagcatt tgcaattgtt tttttggtta
     2341 aaaaattctt tttcaaaaag aaaaaaacca acaaaaactg tggcgaagac tgtaattgtc
     2401 agtaatctac tttcacaaaa agattattag ctattttatt cgaaatcgtt aattctgttg
     2461 tttcgtttac tctcattttt gtagataaat cgaagctttc tttggccaga atttcaattt
     2521 ttgaacccaa agcaatagct tgtttgtcta aatatttcaa aaattcggcc gaagaatctt
     2581 ttactcccac acaaattccg atttgattta tagccaaatc cgaaagcaat tgtttatcca
     2641 ttttggccat tcttccctga gcatctggaa tgggatcgcc gtgtggatct tcggttggat
     2701 tgcccaaaaa atcatccaat ttattgatga gttgctcaga tttgatgtgt tctaattgtt
     2761 cggcaatatc atgaacctca tcccaagcaa aatttaattt atctactaaa aaaacttccc
     2821 acaaacgatg tttccttaca atcattttgg ccgaaagtaa tcctttattg gtcaaagaaa
     2881 cgccttggta ttttttgtaa ttcactaaat ctttttcggc caattttttg agcatatctg
     2941 tcactgagga agctttggtt tccatcattt cggcaatagc attagtgctc acatcggcat
     3001 cagtaaccgt tgtaagatga taaatagctt ttaaataatt ttcttctgat aaagtcattt
     3061 gattttagat tacagatttt agatttttaa aatcttaata cttgattctt ttttacttaa
     3121 tatttctttc taatttttta caataaacaa aggaatcgaa attttgtgtc ttaatttatc
     3181 tacggtggtt ccaaaaatca aatccttgaa tccagtgtga ccgtgagttc ccattactaa
     3241 aacatcaaaa ttgcctttgt tgatgatttc aggaatcact ttattgggct tgccataccc
     3301 taattttgtc tttactgtaa agcctttatg cgaaagcatt gccttgtatt ctgccaataa
     3361 ttttgcatca attagggttt cgtgatcttc aatattttta ccataaacca ttgcccccac
     3421 tgtttctacc acgtgaatca aggtatattt tgcctcaatt ccgcccaatt caaaagcact
     3481 gtttatggca atagtatcgg ccgaggagaa atctacagca attgcgattt tctttgtatt
     3541 gtaggttcca attttagaaa attgaagatt taaattgtgt ggcgaatgat tttgaaattc
     3601 ctgttttgct ttggttataa acggtttgaa aacgatatac aataacaaaa tcagaaatcc
     3661 aaaagctaat ggaacgaccg taagccacaa cacgattggg ttttctgaag tttcgagcca
     3721 gccaacaatt tcgttaaaaa ccaatttggc attcaaagaa acaataatgg tggcaataat
     3781 ccaagcagca acttgagtaa ttttagaaat atgaaatcct ttcattttgg ttttatcact
     3841 cacaaaatga atgagtggaa ttatggcaaa acccaattgc aaactcaaaa tcacctgact
     3901 caaaatcaat aatttccccg ttacgctttc gccataaatt aaaatcacaa ctaccgcagg
     3961 cacaatggca atcaatcggg taataattct tcgcacccaa ggttgcattc taaagttcaa
     4021 atagccttcc ataacgattt gtcctgccaa agttcctgta atggtagaac tttgtcccgc
     4081 agcaatcaag gcaactgcga ataaaatggg tgcccatttt gttcctaaaa gtggttgcaa
     4141 gaactgatgt gcatcttgaa tttcggctac ttcaaaataa ccgtttttat aaaatgtggc
     4201 tgccgccaaa attaaaatag cagcgttcac aaaaaatgcc aaattcaaag caatagtcga
     4261 atcgatgaaa ttgtatttca aagcttgttt aattccatct ggagttctgt caaattttcg
     4321 ggtttgtacc aatgaagaat gcaaatataa attgtgaggc ataaccgttg cacctatgat
     4381 tccgatggca atatacaaag cagtttccgt tggaatggat ggaatcaaac cataaacgac
     4441 tttgtctaat tcgggctggg caaaaatcat ttcgaaaacg aaagaaaacc caataatagc
     4501 gaccaataca ataataaaag cttccatttt tcggatgcct ttattgatta aaaaaagtag
     4561 taaaaatgta tctaaaacag tgattaaaac cccttcgata agcggaatat caaacaataa
     4621 attaattccg attgccattc ctagaacttc agccagatcg caggcagcaa tagcaatttc
     4681 ggccaaaaaa tataaaatat aattaatagg tttcgaatag gtttctcttg aagcttgcgc
     4741 caaatcgcgc tgagtcacga ttcctaatct ggcgcttaaa ctttgcaaaa gcaatgccat
     4801 catattactc atcaacaaaa cccaaagcaa agaatagcca aactgacttc ctcccgcaat
     4861 atcagtagcc cagtttccgg gatccatata gcctacactg accaaataag caggcccaaa
     4921 aaaggccaat atttttctga aaatggaagt tttattttgt gtctgaaccg attgattgac
     4981 ctcttctaat gatttgctca tagctgaaat tgaattgtta aggcaaatat atacaaataa
     5041 attttgtttg ggtaattatt tttttagctt tgtctaaaat ttaatttact caaatgaaat
     5101 atatattttt acttatatca ttcttttcaa ttaacctatt gttttcacaa aacacaattc
     5161 caaaaacaga aaatgattct attgaaaaga aagacaatca aaatgaacta aacgaagttg
     5221 tggtttcggg gactttaaaa cccataagaa gactcgaaag tgccgttcct gttgaagttt
     5281 attctcctgt tttcttcaaa aaaaatccaa ccgccagtat ttatgaagct ttgcaaaatg
     5341 taaacggagt acgacctcag ctcaactgtg gcgtttgcaa cacaggcgac attcacatca
     5401 acggattgga agggccttat acatcggtaa tgatcgatgg aatgccaatt gtgagcagtt
     5461 tatcaactgt ttatggattg tctggaattc cgaattcttt ggtcgaacga atcgaaattg
     5521 tgaaaggccc agcttcctct ttatacggaa gtgaagctgt gggcggatta atcaacatta
     5581 tcaccaaaaa tccaaataat gctcccctat tttctgccga tgtttttaca acttcttggc
     5641 tagaaaccaa tgctgatatt ggcggaaaat ttaatattgg caaaaaagcc acttcaatat
     5701 tggggttgaa ttattacaat tacaatcaaa ctattgacaa cgacaatgat agttttaccg
     5761 atgtaacggc tcaaaacagg atttcggttt ttaataaatt gaatttttct cgaaaccaaa
     5821 accgattatt tacgattgcc gttcgcggaa tgtacgaaga tcgttggggt ggcgatgtac
     5881 gttgggagaa aaaataccgt ggtggcaatg aaatttatgg cgaaagtatt tataccaaac
     5941 gaggcgagct gataggaagt tatcaattgc ccaccaccga aaaactaatg ctttcttttt
     6001 cgggaacaat gcattttcag gacagccgct atggtacaac ttcttatatt gccaatcaaa
     6061 aaattggttt tgcccaacta acttgggaca agaaaattca aaacaacgat ttattggctg
     6121 gaattgcttc tcgctatacc tattatgacg acaacacaac agccaccgct attttaaccg
     6181 aaaacaatcc tgaaaaaacc tggcttcctg ggatatttct tcaggacgaa attactttta
     6241 gcgaaaaaca caaagtcctt ttaggcattc gatacgatta caattccatt cacggaaaca
     6301 ttttcacgcc aagatttgcc tataaagtaa aattcaacaa caataatatc cttcgattga
     6361 atgctggaac aggttttaga gtagtcaatc tgtttactga agatcacgct gccttaactg
     6421 gttcgcgtga agtggtaatt gccgaaaatt tagaccccga aaaatccatc aatgccaatc
     6481 tgaattacat caagaaaatc tatttggaca atggcacatt tttaggcatt gaaactactg
     6541 ctttctacac ccgattcagc aacaaaattg tatccgatta tgaaattgat cccaataaaa
     6601 tcatttatga caacattgat ggttatgctt tgagccaagg gattagctgt aatacggata
     6661 ttaatttcac gaatggatta aaatttattt tgggagcgac ttatatggat gtttccaatg
     6721 tggaaaacgg cattaaaaca agaccgcttt tgaccgaaaa tttcacagga acttggacag
     6781 tttcttataa aatagacaaa ataaaaacca taattgatta tactggaaat gtgtacagcc
     6841 cgatgaaatt gcctttacta agcgaaaccg atccaagaaa tccaaattcg ccttggtaca
     6901 gtctgcagaa cattcaattt acctattcag gctggaagaa tttcgaactc tacgctggca
     6961 tcaaaaacct gctaaatttt acgcccaaac gaaataatcc gttcttgatt tctagaaccg
     7021 aagatccatt cgacaaaaat gtacaatatg attcgaatgg aaaagtattg gtaactcctg
     7081 ataatccgta tggtttaacc tttgacacga cttatgttta tggtcctaat caggggattc
     7141 gaggtttttt tgggttgcgg tataattttt attaaactac ttaaaagcat agccgctgat
     7201 tcacagattt tcaaaaatta tttttaatct gcaaatctgt ggctaaaaaa attaaatgaa
     7261 aaaaagtctt tatatattat taattttctt ctggacaatg ccatctggtt ttgcccaatt
     7321 gaaaacccat acttttgaag aagccgaaaa actttctaaa gaaaatccaa aaccatttgt
     7381 gatttttatt aatacctctt ggtgtaatta ctgcaaaatg atggagaatt ccactttcaa
     7441 aaaccctgaa atcattagtc ttttaaattc agatttctac ttcatttcat tggatgccga
     7501 aagtaaatca gacattcatt tcaataatca tacttttaaa tttcaaccca aaggacaaaa
     7561 tactggaatc cacgaactcg ctacacagtt agcaactatc gattcgcaag taatgtatcc
     7621 aacacttact gttttacaat cggatttttc gatagttttt caaaagcatt cttttttgaa
     7681 ggcgaaagag ttaatcataa ctctggaaaa attaaaataa ctgtatcctt atcaaactat
     7741 aacaaaactt tataattcaa acttatatct tgatttgaaa cttcgtaact ttgcaactta
     7801 tcaaacaata atcttaaaat taaaaaatat gtatccacaa gaaatggtat tgccaatgca
     7861 agctgaatta acttcagtag gttttcaaga tttacataat gccgatgcag taaatgatgc
     7921 aataaaagcc gaaggaacaa cattggtggt ggtgaattca gtttgcggtt gtgctgctag
     7981 aaatgcacgt ccaggagcag caatgagttt agaaggtgcc aaaaaaccag atcatttagt
     8041 gactgttttt gcaggagttg acaaagaagc ggttaatgct gcaagagaac atatgtttcc
     8101 ttttcctcca tcttcaccaa gtattgcctt gttcaaaaac ggagaattgg ttcacatgct
     8161 agaacgtcat catatcgagg gtcgtcctgc cgaattaatt gcagacaatt tgaaagacgc
     8221 tttcaacgag tattgctaaa cttttttggc tcaaaattta tcaaaagcac tatttattta
     8281 gtgctttttg cgtttaaata attaccatcc atcaatagcc cctattgaac tcattctgag
     8341 ctcttcttgt tcgttgcaat ttctagataa attagctact tttgcaaaaa tatttctcat
     8401 cttaaacctg tttatagcat gcaactgtac aacactttaa gcgcagagga aaggactgtt
     8461 atgattgacg atgctggaaa gcaacgtctc accttgtctt tttatgccta tgcgcaaatt
     8521 caaaatccaa cccaatttcg taacgattta tttctagctt ggaatccgct tgaggtttta
     8581 ggtcgaattt atgttgccca cgaaggcatt aatgctcaat tatcattgcc cgcagataat
     8641 ttctatgcgt tcaaagacac tgtcgaagct tatgatttta tgaagggaat gcgattgaat
     8701 attgccgtag agcacgatga tcattcgttt ttgaaattga ccataaaagt gcgtgacaag
     8761 attgttgccg acggtttaaa cgacgctact tttgacgtaa ccaatatcgg aattcattta
     8821 aaagccaaag aatttaatga aattttagaa aatccagaca ctatcgtagt cgattttaga
     8881 aatcactatg aaagcgaaat tggctatttc aaaggagcta tcacacccga tgtagatact
     8941 tttagagaat ctttgcccat cataaacgag cagctaaagg atttcaaaga agataaaaac
     9001 ttagtgatgt attgcacagg aggaatccgt tgtgagaaag cttcggccta ttttaaacac
     9061 cagggtttta aaaatgtgta ccaacttgaa ggcggcatca tcaattatgc caaacaaatc
     9121 aaggaagaaa atctggaaag caaattcatt ggtaaaaact tcgttttcga tcaccgtctg
     9181 ggcgaaagaa ttacagacga tatcgtttcg caatgccatc aatgtggtgc accttgcgat
     9241 gttcatacca attgtttgaa cgaaggctgt catttacttt ttattcaatg tgagtcttgc
     9301 aaagaaaaaa tgcacggctg ctgttcaacc gaatgtgtga ctgtaattca tctcccagaa
     9361 gaggaacaaa aagccattcg aagaggcgtt aaaaacggaa acaaaatctt caaaaaagga
     9421 aaatcggatg ttttgacttt caaaaacaac caagatcctt tggcgaatgt tccaaattta
     9481 agcgaactca taaaatcaaa accgctcctt aaaaaagagc caaaaataaa aaaacaatac
     9541 ctcggaaaag gcactcactt ttttccaaaa actaatattg ctcaattttt aatcgaagaa
     9601 aaccaaatta atattggcga ttccattcta atcaaaggtg ctacaactgg cgaagaaatg
     9661 ttgattgtcg aaaaaatgtt ggtcaatgat gtcgaatctc aaaaagctgt ttctggtgaa
     9721 atggtgactt ttaaagttcc ttttagaatt cgattatcgg ataaattata taaaatttta
     9781 aactagtttt taaagaaatt cacccatcat ctttgtgaaa ttttgaactt tgacaaagat
     9841 ttacatccta taaaatgaca acaaataaca aaatagaatt aatggctccg gccggaaact
     9901 tcgaatcctt gcaagctggt ttagacaatg gtgccgattc cgtgtatttt ggagtagagc
     9961 aattgaatat gcgcgcccga gcaacagtaa atttcacaat ggatgatttg aaggaaattg
    10021 ctcgtcgctg tgaagaaaaa aacgttcgaa gttatttgac tttaaacacc attatttacg
    10081 atcacgattt gtcggttgta aaaacattgt taaacaaagc caaagaagcc aatattacgg
    10141 cagtaattgc ttcagaccaa gccgtgattg caatggcaag aggcattgga atggaagttc
    10201 atatttcgac ccaattgaat attaccaata tcgaaaccat aaaattctac agcctttttg
    10261 cagatacaat ggttttgagt cgagaattga gccttcgcca agtgaaaaac atcaccgaac
    10321 aaatcataaa agaagacata aaaggcccta acggaaaatt agtagaaata gaaatttttg
    10381 gtcacggtgc cttgtgtatg gccgtttctg gaaaatgcta tttgagttta cattccgaca
    10441 attcatcggc caatcgtgga gcttgcaagc aaaattgccg caaaaaatac accgttatcg
    10501 accaagaaac gggtttcgaa attgaagtag ataacgaata tttgatgtcg cccaaagatt
    10561 tatgcacttt agattttctg gaccaagtaa ttgattctgg cattcaagtt ttgaaaattg
    10621 aaggtcgtgg tcgtgcacca gaatatgtgg caaccgtagt aaaaacctac cgcgaagcga
    10681 ttgacagtta ttacgaaggc agtttttcga aagaaaaaat agcggtttgg atggaagctt
    10741 taagcacagt ttacaatcgt ggattttggt ctggctatta tttaggccaa gaattgggcg
    10801 aatggagcga tgtttcagga tcaatggcaa cacaaaagaa agtgtatgtg ggcaaaggaa
    10861 ctcacttttt cccaaaagca gaaattggac agtttaaaat tgaagcctat gacattcaaa
    10921 ttggagacaa gatattagtc acagggccaa gcacaggagc acaagaaatg gttatcgagg
    10981 aaatgtttgt aaatgatgtt acggcagaga aagccactaa aggcgacgat tgcaccttaa
    11041 aattaccttt cagaatccga atgtctgaca aattatataa aatcgtggaa gcctaatggt
    11101 tattgtaaca ctgcaaagag ccaaatgtat tgggtgcaat tattgcgtag aaatggcacc
    11161 cgttcaattt caaatgtcta aaaaagacgg gaaatcggta ttgataaaaa gtgtcaatgc
    11221 caaaggtttt tttaccttaa aatcgcccga ccacactata accgaaagct gtgaattagc
    11281 tgccaaagct tgtcctgtga agataatttc ggtaaaggaa acctaatcta atttattgta
    11341 taaaaaaaac ctctaattct gtaaaaaaga aattagaggt tttttgtttt caaaaacttg
    11401 aaaccttaaa ctttaaactt taaactaaaa ataatatctt taccgattaa aacgtttaag
    11461 aacttaaatt gcgtctatcg caatactaca tataaaatta tggcatgtac aagttgttca
    11521 acctctgatg gcggtgcacc aaagggttgt aaaaataatg ggacttgcgg caccgatagc
    11581 tgcaacaaat tgacggtttt tgactggctt tctaatatga gtttacctaa cggagaagcg
    11641 ccttttgact gtgtagaagt ccgttttaaa aacggaagaa aagaattttt tcgcaataca
    11701 gaaaaattaa cgctaagcat aggagacatt gtcgctaccg aagcatctcc tggacatgac
    11761 atcggaattg ttaccctaac aggcgaattg gttcgcattc aaatgaagaa aaaaggagtc
    11821 aattcttcta atacagatgt tcctaaagta tatagaaaag catcacaaag agacatcgat
    11881 atttggtcta ttgctcgcga tagagaagaa ccgatgaaag ttcgtgctcg tgaattggcc
    11941 atttctcaaa aactagaaat gaaaatttct gatatcgaat ttcaaggtga tggttctaaa
    12001 gctacttttt attacacagc caacgacaga gtcgatttca gacttttaat caaagacttt
    12061 gcgaaagaat ttagcaccag agtcgagatg aaacaagtag gtttgcgtca agaagcagct
    12121 cgtttgggcg gcattggttc ttgcggaaga gaattgtgtt gttctacttg gctaaccgat
    12181 tttagaagcg taaacacctc tgcggcacgc taccaacaat tgtccttgaa tccacaaaaa
    12241 ctggctggac aatgcggaaa attaaaatgc tgcttgaatt acgaactcga cacttacatg
    12301 gatgctctaa aagggtttcc ggactttgac actaaattag taactgaaaa aggcgatgcc
    12361 atttgccaaa aacaagatat tttcaaagga ttgatgtggt ttgcttatac caatgatttt
    12421 gccaattggc atgttttaaa aatagatcaa gtaaaagaaa ttgttgccga aaacaaactc
    12481 aaaaacaaag tagcttcact cgaagacttt gccatagtaa ttgttcaaga gcctgaaaaa
    12541 cattttaata acgcaatggg tcaggaaagt ttaacccgtt ttgatcagcc taagaaaaag
    12601 aaaaaaccaa ataaaaaacg taaaaacggc gaaaatttaa ttgttgctaa taataacaat
    12661 gttaatgcta atacaaatag caatactact agcaaaccta acaacaataa taaacctaat
    12721 aacaacaggc ctaataacaa caacagaccc aataacaaca agcctaaacc caataacaac
    12781 aataacacta acgagaaaaa acctgtagtt gcgagaaaac ctataattat tactaaaaat
    12841 gagaataagg aatagcgcaa tattgctttt agttgtaatt ctcttttttt cgtgcgacaa
    12901 aaaaagagtt ttcgacgaat acaaatccgt aggaacagct tggcacaaag acagtattgt
    12961 ttcatttaat ttacccgaat tagattcgac aaaacgatac aatctttttg taactttgag
    13021 agacaacaac aactatccgt tcaacaatct gtttttgatt gtgggtctcg aaatgcccaa
    13081 tggatttaca aaagtagata cacttgagta cgaaatggcc aatccagacg gatctcttat
    13141 gggagaagga ttttcagaca tcaaggaaaa caaacttttt tataaagaaa aagttcgctt
    13201 tagaggaaaa tacaaagtca acatcaaaca agcagttcgc gaaaacggaa aagttccagg
    13261 tgtaaccgct ttagaaggaa tcacagaagt aggttttaga atagaaaaaa tagattaaaa
    13321 aaatatggct actcaaaaga aaaatattca gacaaaaaat attgaaaaag acatcaccta
    13381 ctataaaaag aaattttgga aagttttctt ttatggcctt ggaggattat tattattttt
    13441 cctatttgcc tcttggggac tttttgggtc tatgccttcg tttgaggatt tagaaaatcc
    13501 cgattctaat ttggccaccg aaatcatctc gtctgatgga gtgattttag gtaaatattt
    13561 cgaaaaaaac aggtcgcaat taaaatattc tgacttacct aaaaatctgg tagatgcact
    13621 ggtagcaact gaagacgaac gtttttatga acattcagga attgatggac gaggaacatt
    13681 aagagccatt gccagtttag gaacaagtgg cggagcgagt acgctaaccc aacaattagc
    13741 caaacaatta ttccacggcg aaggttcaaa attcttgcct tttcgaatcg ttcaaaaaat
    13801 caaagaatgg attatcgcca tcagattaga aagacaatac accaaaaacg aaatcattgc
    13861 catgtattgc aatgtctatg attttggaaa ctattctgtt ggagtaagtt cggcagccca
    13921 aacctatttt tcaaaagcac ccaaagattt aaccatagac gaatcggcaa ttttggtcgg
    13981 aatgttcaaa aattcaggtt tatacaaccc tgtaaaaaat ccacaaggag ttaaaaaccg
    14041 aagaaatgta gtgctttcgc aaatggaaaa aggaggtatc attactcaag gtcaaaaaga
    14101 aaaacttcaa gcattaccaa tcactctgca tttcaaacta gaaagccacc gcgaaggaac
    14161 agctacttat ttcagagaat atttgcgtga atacatgaaa aaatggatgg aaaccaacaa
    14221 aaaagccgat ggatccgatt ataacatcta caaagacggt ttaaaaatct acaccaccat
    14281 cgattctaga atgcaattac atgccgaaga agccgttgcc aaacatatgg aaaacctgca
    14341 agaacagttt tttgaagaag ctaaaaccaa taagaatgca ccattcgtaa acattaccga
    14401 ggccgaaacc caacgcatac tcaaacaagc catgaaagct tcctaccgat ggacagtgat
    14461 gtccgaaatg gaaaaatccg aagaagatat aatagcctcg ttcagcaaaa aaacaaaaat
    14521 gaaagtcttc acttggaaag gcgaaagaga caccattatg actcctttag actccatccg
    14581 atattacaaa cactttttgc aatcgggttt aatggctatg gaaccacaaa cgggtaacat
    14641 caaagcttgg gtgggtggaa tcaattacaa atattttcaa tacgatcacg taggtcaagg
    14701 tgcacgacaa gtaggctcta cattcaaacc atttgtgtat gcaaccgcaa tcgaacaact
    14761 aggaatgtct ccctgtgata ccatcctcga tggaccgttt atgattcgaa aaggacgtca
    14821 tcacgtaacc gaagattggg aaccaagaaa ttctaacaat caataccgtg gcatggttac
    14881 tttgaaacaa ggattagcca attctatcaa caccatttcg gccaaatt
//
LOCUS       DAOSNN010000030        14925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_4030_length_14925_cov_6.196907, whole genome shotgun sequence.
ACCESSION   DAOSNN010000030 DAOSNN010000000
VERSION     DAOSNN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_14925_cov_6.196907"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1567
                     /gene="lpdA"
                     /locus_tag="PLQ70_02865"
     CDS             179..1567
                     /gene="lpdA"
                     /locus_tag="PLQ70_02865"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQE33584.1"
                     /translation="MKYDVIVLGSGPGGYVTAIRASQLGFKVAVIEKENLGGICLNWG
                     CIPTKALLKSAQVFDYLKHASDYGLTVSSFDKDFPAVIQRSRNVAAGMSKGVTFLMKK
                     NKIDVINGFGKVKPGKKIDVTDAAGKVTEYSADHIIIATGARSRELPNLPQDGVKVIG
                     YRQAMSLPTQPKKMIVVGSGAIGVEFAHFYNSMGTDVTIVEFMPNVVPVEDEDISKEF
                     EKSLKKAGINVMTNSSVEKIDTSGAGVKAFVKTAKGEEILEADILLSAVGIKTNIENI
                     GLEEVGIAVDRDKILVNAYNQTNIPGYYAIGDVTPGQALAHVASAEGINCVEKIAGLH
                     VDPIDYGNVPGCTYATPEIASVGLTEKAAKEKGYELKIGKFPFSASGKAKAAGAADGF
                     VKVIFDAKYGEWLGCHMIGAGVTDMIAEAVVARKLETTGHEILKSIHPHPTMSEAVME
                     AVAAAYGEVIHL"
     gene            1855..2349
                     /locus_tag="PLQ70_02870"
     CDS             1855..2349
                     /locus_tag="PLQ70_02870"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQE33585.1"
                     /translation="MKKMNFIKIILAALILISFSCKKAAESIETAKTTFDLEAAKSAI
                     ESAGENFVIAFNKGDSIGLANCYTTDAKMMGPNEKSIIGRPAIQKVFSGWIKSGTPTF
                     TMKTIEVWGNEDLMAAEEEWTFSDKNGKILDSGKSIELFKMEDGKWRLYRDCYNSDMP
                     IPKN"
     gene            complement(2418..2723)
                     /locus_tag="PLQ70_02875"
     CDS             complement(2418..2723)
                     /locus_tag="PLQ70_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33586.1"
                     /translation="MIVQYQGKEKEIPTKFSIRIIGNNLSKSSSNYQLELLVGDKQIP
                     TFTTLIHQSLYNCLKEIYLFRKYNQMVFDDASEKIISEYVPYDLVLYSHEKKVLFMA"
     gene            complement(3137..4909)
                     /locus_tag="PLQ70_02880"
     CDS             complement(3137..4909)
                     /locus_tag="PLQ70_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein"
                     /protein_id="HQE33587.1"
                     /translation="MNLYQNKITHYSQALEKINTKYNTISILRILSVFASLFFLFNYI
                     KNSEIFYAIAAFLSFVCFVFLMRIHSRLSFQKQLTSTLLTINKNEISYLKREKIPFEN
                     GIEFNDFHHPYAYDLDIFGEHSLFQNLNRTATFIGKKTLANQLLILNSNEAILENQEA
                     INELKTKLDWRQEFFALATISNDSKNVYESLLHWNSFKNNSLSKFILVLSFALPTLFL
                     GLSVGYYLTSKTILLSFLAYVFIANLMVVGKLLKRIQSEIAKADRVDKVIKQYSLLLK
                     KIEGETFQSKKLNDLRRQLISNHTTASTHLKKLSELFSSMDTIANIVVATLFNGIFLY
                     HLHVLKSILNWKINYSTQLEQWVIVIGEFEMLNSLANLAHNNPDFVFPEINSEYKIGF
                     KDLGHPLLNPATRVGNDTYFYPQSFVILTGSNMSGKSTFLRSLGINMVLSGMGSVVCA
                     SKATVHPLPVLVSMRLSDSLSDSESYFFAEIKRLKQIMDVLEGKPAFVLLDEILRGTN
                     SDDKRNGTIEVVKKIIAQKAIGAIATHDIEVCLTTNSYPEILTNQCFEVEIRDNELFF
                     DYKLRNGICKNKSATFLMQKMGVI"
     gene            5090..6040
                     /locus_tag="PLQ70_02885"
     CDS             5090..6040
                     /locus_tag="PLQ70_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HQE33588.1"
                     /translation="MQNIPSVDLRDFLSDDPKRKQKFVNEIGSAFEEIGFVALKGHFL
                     NDQLVEELYGEIRNFFALPLETKHGYEIPGIGGQRGYVSFGKEHAKGRKEGDLKEFWH
                     FGQYVEKDSKYASEYPDNVEVKELPRFNIVGKEAYQMLEKTGVYVLRALALHLGLDEF
                     YFDEYAKEGNSILRPIHYPPITTEPENAIRAAAHGDINLITLLMGAQGRGLQVQNHDG
                     DWIDAIAEPDELVINVGDMLSRHTNNKLKSTIHQVVNPPRELWGTSRYSIPFFMHPVS
                     DMKLDCLENCIDAENPKQYEDITAGEFLYERLVDLGLIKK"
     gene            6207..6536
                     /locus_tag="PLQ70_02890"
     CDS             6207..6536
                     /locus_tag="PLQ70_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HQE33589.1"
                     /translation="MDLQEQLKNLFPDHIESEPEEIQEEEHTLFIQKEPMICKFEKRK
                     GKATTIIEGYEGTDEDFKILAKEIKTKLSVGGTFKDDSIIIQGDYRDKIMQILKEKGF
                     KVKRVGG"
     gene            6622..7488
                     /locus_tag="PLQ70_02895"
     CDS             6622..7488
                     /locus_tag="PLQ70_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HQE33590.1"
                     /translation="MIQSSELILNPDGSVYHLNLLPENIAHDIIFVGDQNRVEKITQF
                     FDSIEFSTQKREFKTQTGLFKGKRITVMSTGIGPDNIDIVMNELDALVNIDLKTRTPK
                     ENLTSLNIIRIGTSGSLQADIPVDSFVMGKFGLGLDNMLRSYLIDEISEIAMEDAFIQ
                     HTNWDLRKGRPYIIACSKKLEKRMESNRIFKGITGTAGGFYGPQGRVLRLNIQDENLN
                     SKMDSFNYEGTRMTNLEMETSAIYGLGKLLGHNCLSLNAIIANRANGTFSDDPYKAVD
                     ELITYALGKLAE"
     gene            7759..8289
                     /locus_tag="PLQ70_02900"
     CDS             7759..8289
                     /locus_tag="PLQ70_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE33591.1"
                     /translation="MKLILETDRLILREFLLSDAEAFFAMDSNPNVHQYLWNKPVQKM
                     EETIEIIEFVRKQYTENGIGRFAMILKETNEFIGWAGLKFNTEIVNNKTNFYDIGYRL
                     DEKFWGKGYASEATFAWLKYAFETMNIKTIEAAAHTENGASNRILQKIGMQMTEQYLE
                     DGISWNWYQLENNNSF"
     gene            complement(8311..8571)
                     /locus_tag="PLQ70_02905"
     CDS             complement(8311..8571)
                     /locus_tag="PLQ70_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33592.1"
                     /translation="MFGDTKDTIPTNIKIYNKVGDAYGTMIDCAYIVDAENKVEFMVS
                     ATLLVNKDGVFNDDKYEYDTIGLPFLGELGRQLYAKNLKLKK"
     gene            complement(8532..9449)
                     /locus_tag="PLQ70_02910"
     CDS             complement(8532..9449)
                     /locus_tag="PLQ70_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQE33593.1"
                     /translation="MKSIRVFILIALVFLEFGCSTTKKNILQETLASDNLKIKNVMSN
                     PEAFELQIIYTQIIRDKKNKVSFKDFTYRLNAKNYFYPASTIKFPIAILALEKLNTIE
                     NTSKDSQYTIEGNPEKLQFSKEISKVFAVSDNEASTNLLEFLGFDFVNESMKAKGLQP
                     FRLSHRVSGSDPRSLATKPILLYKNDGSKLQIPAVSSQPSIPLNLKNIKKGIGYIKND
                     SLVNGSFDFSSKNYYPLETLHNTMKRIVFPETFKESERFKLTEKDRQFILFSMQNLPK
                     NAGYNSTEYYDGYCKFLCLVIPKILFQPI"
     gene            complement(9460..10131)
                     /gene="ung"
                     /locus_tag="PLQ70_02915"
     CDS             complement(9460..10131)
                     /gene="ung"
                     /locus_tag="PLQ70_02915"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQE33594.1"
                     /translation="MQPNLNPSWQTILSDEIQKSYFQDLMNSVDEEYQNYTCFPPKEL
                     LFAAFDYCSFQDLKVVIIGQDPYHGVNEANGLCFSVNDGIKIPPSLRNIFREMNNDLG
                     TIFFPNSGNLEPWAKQGILLLNAALSVRMDAPNSHKHLKWSAFTDAIIQKISDEKENV
                     VFLLWGAFAQKKGAKIDRAKHLVLESGHPSFANVHKKWFGNKHFSLTNLYLEEKGIAP
                     IEWNF"
     gene            10303..12468
                     /locus_tag="PLQ70_02920"
     CDS             10303..12468
                     /locus_tag="PLQ70_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQE33595.1"
                     /translation="MISITQKTLQDLQFPTVLETISEICNTDIGKQKALEITPFRDKE
                     TLINALMQTSEYVSSFQNNNAIPNHGFDAITYEIKFLAIEDSFLEVGGFRKIATISDT
                     TNFLLNFLKKFDDYYPYLNRKASEIQITKEISSLIDVVVDKYGEIRDNASPQLSEIRK
                     SMNLVRGKVNQSFGVALTQYNSLGYLDDIKESFVQNRRVLAVLAMYRRKVKGSILGSS
                     KTGSIAYIEPEATLRYSRELSNLEYEEKEEITRILKQLSNQIRPFLPLLIQYQDFLSD
                     IDVVAAKAKYANRINGILPTITENRRLFFRDAFHPILYLNNKQKNEITHPQTIELSQE
                     NRIIVISGPNAGGKTISLKTVGLLQLMLQSGMLIPVHERSETFLFDRILTDIGDNQSI
                     ENHLSTYSYRLKNMNYFLKKCNKKTMFLIDEFGTGSDPELGGALAEIFLEEFYHREAF
                     GIITTHYSNLKILANELPFATNANMLFDEKTLEPMYKLVLGQAGSSFTFEVALKNGIP
                     YGLINRAKKKIEVGKVRFDKTIATLQKERSKLEKTSINLKEEETRAREEGKKMENINL
                     KIKQKLESYQELYDSNQKTIYIGQKIEDIAEKYFNNKNKKDLIGEFLKLVEIENSKRK
                     KTTPKVAKAIIEKKKEIIKEVEIKVEEIRIEKKEKKLKPVIEKPKPILKVGDRVRMMD
                     GKAVGSIDKIEKNKAVVNYGLFTSKVSLEELELVEAVKK"
     gene            12642..13055
                     /locus_tag="PLQ70_02925"
     CDS             12642..13055
                     /locus_tag="PLQ70_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HQE33596.1"
                     /translation="MQNLPKNKKIILFDGVCNLCNSAVQFVIRHDKKDVFLFVALQSE
                     LGQDILNHIGINPKNIDSIILYEPGIAYYYKSQAAIEIARNLGGFWHFGTIFRIIPTP
                     IANQLYDYVAKNRYKWYGKKESCMMPTPELKSKFL"
     gene            complement(13337..>14925)
                     /gene="ettA"
                     /locus_tag="PLQ70_02930"
     CDS             complement(13337..>14925)
                     /gene="ettA"
                     /locus_tag="PLQ70_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HQE33597.1"
                     /translation="AKIGILGLNGTGKSSLLKIIAGVDKNYQGDVVFQPGYTVGYLEQ
                     DPQLDDSKTVLEIVQEGVAETMAVLDEYNKINDLFGLEENYSDPDKMDKLMDRQAALQ
                     DKIDALGAWEIDTKLEIAMDALRTPDGDTPIKNLSGGERRRVALCRLLLQQPDVLLLD
                     EPTNHLDAESVLWLEQHLAQYSGTVIAVTHDRYFLDNVAGWILELDRGEGIPWKGNYS
                     SWLDQKSNRMAQEEKVASKRRKTLERELEWVRQGAKGRQTKQKARLQNYDKLLNEDQK
                     QLDENLEIYIPNGPRLGTNVIEAKGVAKAFGDKLLYDNLNFTLPQAGIVGIIGPNGAG
                     KSTIFKMIMGEEKPDGGEFLIGDTVKIAYVDQAHSNIDPNKSIWENFADGQELVMMGG
                     KQVNSRAYLSRFNFGGGEQNKKVSMLSGGERNRLHLAMTLKEEGNVLLLDEPTNDLDV
                     NTLRALEEGLENFAGCAVVISHDRWFLDRICTHILAFEGNSEVYYFEGGFSDYEENKK
                     KRLGGDLTPKRLKYRKLIRS"
BASE COUNT         5149 a         2604 c         2564 g         4608 t
ORIGIN      
        1 cctatttaaa aatgaaaccg taagtttcta aataattaac aaacttatgc aagagtggaa
       61 gtttttttta aaataaatcc ttaattttgc caactgaaat taattttgat agctgatcaa
      121 aaatggcacg agtttttgcg actgcatttt taccataatt taaataataa gaaaaaaaat
      181 gaaatacgat gttatagttt taggaagtgg gccaggcgga tatgtaactg ccattagagc
      241 ttctcaatta ggctttaaag ttgccgtaat cgaaaaagaa aatttgggtg gaatttgttt
      301 gaactggggt tgtatcccta caaaagcatt gttgaaatcg gctcaggttt ttgattactt
      361 aaaacacgct tcggattatg gattgactgt ttcatcattc gataaagatt tccctgctgt
      421 aattcaacgt agccgtaatg tggctgctgg aatgagcaaa ggcgttactt tcttgatgaa
      481 aaaaaataaa attgacgtta ttaatggttt tggaaaagta aaaccaggta aaaaaattga
      541 cgttaccgat gcagctggaa aagtaacaga atattctgca gaccatatta ttattgcaac
      601 tggagcacgt tctcgtgagt tacctaactt accacaagat ggtgtaaaag taattggata
      661 ccgtcaagca atgagtttac ctacacaacc taaaaagatg attgttgtag gttcgggagc
      721 cattggagta gaatttgcac atttttacaa ctcaatggga acagatgtta ctattgtgga
      781 gtttatgcct aatgtagtcc cggttgaaga cgaagatatt tcaaaagaat ttgaaaaatc
      841 cttgaaaaaa gcaggaatca atgtaatgac aaattcctct gtagaaaaaa ttgacacttc
      901 tggagcaggt gtgaaagcat tcgttaaaac cgctaaaggt gaagaaattt tagaagctga
      961 tattttatta tctgctgttg gaatcaaaac caatatcgaa aacattggat tggaagaagt
     1021 aggaatagct gttgatagag ataaaatctt ggtaaacgcc tacaaccaaa ccaatattcc
     1081 gggttattat gccattggtg acgtaactcc aggtcaagct ttggcacacg ttgcttctgc
     1141 tgaaggaatt aactgtgtgg agaaaattgc tggccttcat gtggatccaa tcgattacgg
     1201 aaacgttccg ggttgtactt atgctacacc agaaattgct tctgttggtt tgactgaaaa
     1261 agcagcaaaa gaaaaaggat atgaattgaa aattggaaaa ttcccattct cagcctctgg
     1321 aaaagctaaa gctgccggtg ctgccgacgg atttgtaaaa gtaattttcg atgctaaata
     1381 cggagaatgg ttaggttgcc atatgattgg tgctggtgtt acggatatga ttgcagaagc
     1441 agttgtagct cgtaaactag aaactacagg tcacgaaatc ttaaaatcga ttcaccctca
     1501 cccaacaatg agtgaagcgg ttatggaagc cgttgctgct gcttatggag aagtaattca
     1561 cttgtaatat cagattactt aaaatattga aatccgtttt gtgaaaacag agcggatttt
     1621 ttttgcggca aattttcttg ttattaaata taattcagta aatttgatag accctaatca
     1681 cttaattaaa acaatatata ccaataagtt atcaaatccc ccactctttt gattgtattc
     1741 ctattaattg atttcgtatt gcatttttag tttcataaaa aaaataaatc attatttgag
     1801 agtcatcaaa ttctcaaact attaaaataa tttattaacc caaaaaactt tattatgaaa
     1861 aaaatgaatt ttatcaaaat cattttagct gcactgattt taattagttt cagttgtaaa
     1921 aaagcagcag aatctattga aactgcaaaa accacctttg atttggaagc agcaaaatca
     1981 gcgattgaat ctgcaggcga aaactttgtt attgctttta acaaaggaga ttctattggg
     2041 cttgcaaatt gttataccac cgatgccaaa atgatggggc caaacgaaaa atcaataatt
     2101 ggaagaccag ccattcaaaa ggtattcagt ggttggatta aatcaggaac gcctactttc
     2161 accatgaaaa caattgaagt ttggggcaat gaagacctaa tggctgccga agaagaatgg
     2221 actttttcag ataaaaatgg aaaaattctt gactctggaa aatcaattga actttttaaa
     2281 atggaagatg gaaaatggcg tctctaccgt gattgctata attctgacat gccaattcct
     2341 aaaaactaag aaactttgac ataaaaaaat ccgttttgtt tggttacaaa acggattttt
     2401 tttgaaatac gaaatgatta agccataaac aaaactttct tctcatggct gtataaaacc
     2461 aaatcataag gaacatattc agaaattatt ttttctgaag catcatcaaa taccatttga
     2521 ttgtattttc taaacaagta aatttctttt agacaattat acaaactctg atgaattaat
     2581 gttgtaaaag ttggtatttg cttgtcacct accaataatt ctaattgata atttgaacta
     2641 cttttcgaaa gattgttccc gataattctt atcgaaaatt ttgttggaat ttctttttcc
     2701 ttcccttgat actgtactat cataattaaa taaattttaa tgttattaaa ttaaaaaacc
     2761 aaattttatt ggttgaatgc tactcaaact actcaaaaaa aaatataaga aaatcaaaaa
     2821 aaagagctcc ttaaataatt catttgaaac aaaactacta aataaatcat ttcaaaactc
     2881 acataaaaga ataaaacttt cacaaataat ttcgaacttt ataaataatc tagactacta
     2941 attctactaa aaatacttta taattatttt tgaattaatt gatttcaaaa aacgcaattt
     3001 atcatttcaa tttttaggac tattgtggat ttataccgtt aaatcgctat tttttttgaa
     3061 tgccaaataa aatttaataa ctcatgaaaa ttttatgttt taacattgat tttcgattct
     3121 atttttaacc aaaacattaa atcaccccca ttttttgcat caaaaaagta gcacttttat
     3181 ttttacaaat accgtttctt agtttatagt caaaaaacaa ttcattgtct ctaatttcga
     3241 cttcaaagca ttgattggtc aaaatttcgg gataagaatt ggtggtcaaa cacacttcaa
     3301 tatcgtgggt ggcaatggct ccaatggctt tttgggcaat tatctttttg accacttcaa
     3361 tcgttccgtt gcgcttgtcg tcggaattgg tgccacgcaa aatttcatcc aacaggacaa
     3421 aagccggttt tccttccaag acgtccataa tttgttttaa gcgtttaatt tccgcaaaaa
     3481 aataggattc gctgtcggac aatgaatcgg ataatcgcat tgaaactaac actggcaaag
     3541 gatgaaccgt agctttcgaa gcacaaacta ccgaacccat tccgctcaaa accatattga
     3601 tgcccaaact cctcaaaaaa gtacttttac cagacatatt cgaacccgtc aaaatcacaa
     3661 acgattgagg ataaaaatac gtatcattgc ctactctagt tgctggattc aacagtggat
     3721 gtcccaaatc tttaaaccct attttatact ctgaattgat ttcaggaaaa acaaaatcgg
     3781 gattgttgtg cgccaaattc gccaaactat tgagcatctc gaattcacca attacaataa
     3841 cccattgttc caattgagtt gaataattga ttttccaatt caaaattgat ttcaaaacat
     3901 gtaagtgata caaaaaaatt ccattgaaca aagtagctac aacaatattt gcgatagtgt
     3961 ccatactcga aaacagctct gacaactttt ttaaatgtgt acttgctgta gtatgattgc
     4021 ttattaactg ccttcttaga tcgttcagtt tttttgactg aaacgtttcg ccttcaattt
     4081 tctttagcaa taaactatac tgtttaatga ctttatccac cctatctgct tttgcaattt
     4141 cagattgaat tcgtttaagt agctttccaa caaccattaa attagcaata aagacataag
     4201 caagaaaaga cagcaaaatg gttttcgatg tcaggtaata acctactgaa agtcctaaaa
     4261 acaaagtggg caacgcaaac gaaagtacta gtataaactt agacaagcta ttgtttttaa
     4321 acgaattcca atgcaataaa gattcataaa cattttttga atcattgctt attgttgcca
     4381 aagcaaaaaa ttcctgacgc caatccaatt tggttttgag ttcgtttatg gcttcctgat
     4441 tttcgagaat agcttcatta ctattcaaaa tcaataattg attggccaat gtttttttac
     4501 caataaaagt agccgtccta ttcaagtttt gaaacaagga atgttcccca aaaatatcca
     4561 aatcataagc atatggatga tgaaaatcat tgaattctat tccgttttca aatgggattt
     4621 tttctctttt cagatacgaa atttcatttt tattgatggt taaaagagtc gaagtcaatt
     4681 gtttttgaaa agacaaacgg ctatgaattc gcatcaaaaa aacaaagcaa acaaaagata
     4741 aaaacgcagc aatagcatag aaaatctcgc tatttttgat ataattaaac aagaaaaaca
     4801 aactagcaaa aacacttaaa atccgtaaaa tgctaatggt attgtatttg gtattaattt
     4861 tttcgagagc ttgagaataa tgggtaattt tattttgata gagattcatt ttcttgattt
     4921 caatggaata caaatatagg tctcattctg cttaaatcag ttacatattt tcaaatattc
     4981 gtttttaaac ttatgcctaa tcatcatata aaacgaatgt ttttattaaa attattttct
     5041 acatttgtat ggaaaggcca atgcttttcg tgtttaaaaa aataaaacta tgcaaaacat
     5101 tcccagtgtt gacttgcgtg atttcctttc ggatgacccg aaacgtaaac aaaaatttgt
     5161 aaatgaaatc ggaagtgcct ttgaagaaat tggctttgta gccttaaaag gccatttttt
     5221 aaacgaccaa ttggtcgaag agttgtatgg cgaaatcaga aacttttttg ccttgccatt
     5281 agaaaccaaa cacggttatg aaatccccgg tattggcggt caaagaggct atgtttcttt
     5341 tggtaaagaa cacgccaaag gccgaaaaga aggtgatttg aaagagtttt ggcactttgg
     5401 acaatatgta gaaaaagatt ctaaatacgc ttcggaatat cctgataatg ttgaagtaaa
     5461 ggaattacct cgttttaaca tcgttggtaa agaagcctac caaatgttgg agaaaacagg
     5521 cgtttatgtc ttgagagcat tggcattaca ccttggtttg gacgaatttt attttgatga
     5581 atatgccaaa gaagggaatt ctattttacg tcccattcat tatcctccca ttacgacgga
     5641 acctgaaaat gcaattcgtg cagcagccca cggcgacatc aacctgataa cccttttgat
     5701 gggtgcacaa ggtcgaggtt tgcaagtgca aaatcacgat ggtgattgga ttgacgccat
     5761 tgcggaacct gatgaattgg taattaatgt aggtgatatg ctatctcgcc acaccaacaa
     5821 caaattgaaa tctacgattc atcaagtggt caatccgccg agggaattgt ggggaacgtc
     5881 ccgttattcc attccgtttt ttatgcatcc cgtgagcgat atgaaattag attgtttaga
     5941 aaattgcatc gatgccgaaa atccaaagca atacgaagac atcacagcag gagaattcct
     6001 gtatgaaaga ttggtcgatt tgggattaat caaaaaataa agattgtaac gcagatgacg
     6061 cggattcgct aaagcgaaaa cacagatata agcggatttt tatttgattt ctatttgaaa
     6121 atcagttttt atccgtgtct ttacgaagta aatccgtgcc atctgcgtac caaaactatg
     6181 ttgagtttta cattaaaaat aaaaaaatgg acttacaaga acaattaaaa aacttatttc
     6241 cagatcacat cgaatcggaa cccgaagaaa ttcaggaaga agagcacacg cttttcattc
     6301 aaaaagaacc tatgatttgc aaattcgaaa agcgaaaagg caaagccacc accatcatcg
     6361 aaggttatga aggaaccgac gaagatttta aaattctggc taaagaaatc aaaaccaaat
     6421 tgagcgttgg cgggactttc aaagatgatt ccatcattat tcaaggcgat tatcgcgaca
     6481 aaattatgca aattctcaaa gaaaaaggat tcaaagtaaa acgcgttgga ggatagaaat
     6541 agagtgttta gtattcagct tttagtgttc agttaaacaa cattaaactt ttaaacctta
     6601 aatattttaa actacctacc aatgatacaa tcctcagaac taatactcaa tccagacgga
     6661 agcgtctatc atttgaacct gttgccggaa aatattgccc acgacattat ttttgtgggt
     6721 gaccaaaacc gtgtcgaaaa aatcacgcaa ttctttgaca gcatcgaatt ttcgacccaa
     6781 aaaagagaat tcaaaaccca aaccggactt ttcaaaggca aacgaattac tgtaatgtcc
     6841 accggaattg gtcccgacaa tatcgacatc gtgatgaacg aattggatgc tttggtcaac
     6901 atcgatttga aaaccagaac gccaaaagaa aatctaactt cattaaatat tatccgaatt
     6961 ggaacttctg gctcattgca agccgatatt cccgtggata gttttgtgat ggggaaattc
     7021 ggattgggat tagacaatat gctccgctcg tacttgattg acgaaatctc ggaaattgca
     7081 atggaagatg cctttatcca acacaccaac tgggatttaa gaaaaggacg tccttacatc
     7141 atcgcttgtt ccaaaaaact ggaaaaacgt atggaaagca accgcatttt caagggaatt
     7201 acgggaactg ccggcggatt ctatggacct caaggacgtg ttttacgatt aaacatccaa
     7261 gacgaaaact tgaattccaa aatggacagc ttcaattatg aaggcacccg aatgaccaat
     7321 ctcgaaatgg aaacctcggc catttatgga cttggaaaat tactcggcca caattgtttg
     7381 tctttaaacg ccatcattgc caatcgtgcc aacggaactt tcagcgatga cccctacaaa
     7441 gctgttgatg aattgataac ttatgctttg ggaaaattag cggagtaaaa atattaaata
     7501 tgattagtat aatagttcat ttatttgttg gaataataca gcgttttttt taggaattgg
     7561 aaggattaca cgctggttta tttttcaaat ctataatgaa tgttttacag aaaaatttcc
     7621 cagaaatata gattattata tagacaacga gagtactaaa aaagataaaa atggtttttc
     7681 agtacaaaat aaaaactttt tttcaggaat aattgttatt gtattaataa taatattgct
     7741 tgataaattt gaaaacaaat gaaattaatc ctcgaaaccg acagactcat tcttcgtgaa
     7801 tttctacttt cagatgctga agcttttttt gctatggata gtaatccaaa tgttcatcaa
     7861 tatttatgga ataagcctgt tcagaaaatg gaagaaacga ttgaaattat tgagttcgtc
     7921 aggaaacaat atacagaaaa tggcattgga agatttgcaa tgattttgaa agaaaccaat
     7981 gagtttatag gttgggcagg attaaaattc aataccgaaa tagtaaataa taaaactaat
     8041 ttctatgata ttggctatcg attggacgaa aaattttggg ggaaagggta tgcaagcgaa
     8101 gcaacatttg cttggttgaa atatgctttc gaaactatga atatcaaaac tatagaagct
     8161 gctgcacata ctgaaaatgg agcatccaat agaattcttc aaaaaattgg aatgcaaatg
     8221 accgaacaat atctagaaga tggtatttct tggaattggt atcaattaga gaataacaat
     8281 agtttctaaa aaccattctt actccaattt tcattttttt aacttcaaat ttttcgcata
     8341 aagttgtctt cccaattctc ctaaaaaagg caaaccaatg gtgtcatact catatttatc
     8401 atcattgaaa acaccgtctt tgttgactaa aagcgttgcc gaaaccataa attcaacttt
     8461 gttttcggca tccacaatat aggcacaatc gatcattgtt ccgtaagcat cgcctacttt
     8521 attataaatt tttatattgg ttggaatagt atctttggta tcaccaaaca taaaaattta
     8581 caatacccat cgtaatattc ggttgaatta tagcctgcat ttttagggag attttgcatc
     8641 gaaaacagaa taaattgtct atccttttca gtcaatttaa accgttccga ttctttgaaa
     8701 gtttcaggaa atacaattct tttcattgta ttgtgaagtg tttccaaagg ataataattc
     8761 ttactcgaaa aatcaaaaga accattcacc aacgaatcgt ttttaatata accaattcct
     8821 tttttgatgt tttttaaatt caaaggaatg gaaggttggc ttgaaacagc tggaatttgg
     8881 agctttgatc catcattttt ataaagtagt attggttttg ttgccaaact ccttggatca
     8941 gaacccgaaa cgcggtggga aagtctaaac ggttgcaatc ccttggcttt catactttcg
     9001 ttcacaaaat cgaaacctaa aaactccaat aaattagtac tggcttcgtt gtcactaacg
     9061 gcaaaaactt tcgaaatttc tttcgaaaac tgaagttttt cgggatttcc ttcaatcgta
     9121 tattgcgagt cttttgaagt attttcaatc gtattcaact tttctaaagc caaaatcgca
     9181 attggaaatt tgatggtact cgctggataa aagtaattct tagcattcag acgataagtg
     9241 aaatctttga aactcacttt atttttttta tctcgaataa tctgagtata aataatttga
     9301 agttcgaaag cttcaggatt tgacatcacg tttttaatct tcagattatc cgaagccaaa
     9361 gtttcttgca ggatattttt ctttgttgta gaacatccga actccaaaaa aactaatgca
     9421 attaggataa aaactcgaat acttttcatc tttaattggt taaaaattcc attcaatagg
     9481 tgctattcct ttttcttcca aatataaatt tgttaggcta aaatgcttgt tgccaaacca
     9541 ttttttgtga acatttgcaa atgaaggatg tccagattcc aaaacgaggt gtttggctcg
     9601 gtcaatttta gctccttttt tttgggcaaa agcaccccaa agtaaaaaaa caacattctc
     9661 tttttcgtct gaaattttct ggattatagc atctgtaaaa gcactccatt tcaaatgttt
     9721 gtgactgttg ggagcatcca ttcgaactga aagagctgcg ttcaaaagta aaattccttg
     9781 ttttgcccaa ggttctaaat ttcctgaatt aggaaagaaa atagtaccca aatcattgtt
     9841 catttccctg aaaatattgc gtaaagatgg cggaattttg attccatcat tcaccgaaaa
     9901 acacaaacca ttcgcctcat taactccgtg atacggatct tgaccaataa tgacgacttt
     9961 caaatcttgg aacgaacaat aatcaaaagc ggcaaatagc agttcttttg gaggaaaaca
    10021 agtgtagttt tggtattctt catcaaccga attcatcaaa tcctggaaat aagatttctg
    10081 gatttcgtcc gataaaatag tctgccaaga aggattgagg ttgggttgca tattcaaaag
    10141 ttatttcaca aagatacacg aagaacacac agagatacac aaaaaaagtt tgaaagtttt
    10201 gtgtgacttt gagtttttgc ctttgtgaat ctctgtgaaa taaacattat gtttctcttt
    10261 gaaataatcc tatttttgta actaaaagaa ttcaagaatt aaatgatatc cattacccaa
    10321 aaaacgcttc aagatttaca atttccaacc gttctcgaaa ccatttcgga gatttgtaat
    10381 actgatattg gcaagcaaaa agcactcgaa atcactcctt tccgggacaa ggaaaccttg
    10441 ataaatgcct tgatgcaaac ctcggaatat gtttcgtctt tccaaaacaa caatgcgatt
    10501 cccaatcacg gatttgatgc aattacgtac gaaatcaagt ttttggccat tgaagacagc
    10561 tttctggaag taggtggatt ccgaaaaatt gccaccattt cggataccac gaatttcttg
    10621 ttgaatttcc taaaaaaatt cgatgattat tatccttatt taaatagaaa agcctccgaa
    10681 attcaaatca ccaaggaaat tagcagtttg attgatgttg tggtcgataa atatggagag
    10741 attcgagaca atgcttctcc gcaattatca gaaattcgca aaagtatgaa tctcgttcgc
    10801 ggcaaagtca atcagagttt tggagtggct ttgacacaat acaattcttt gggttatttg
    10861 gacgatatca aggaaagttt tgttcaaaat cgtagggttt tagccgtttt ggcaatgtat
    10921 cgccgaaaag taaaaggctc tattttgggt agttcaaaaa caggaagtat cgcttatatc
    10981 gaaccggaag ccaccctgag atattcccga gaattgagca atttagaata cgaagagaaa
    11041 gaagaaatca ctcgaattct gaagcaatta tcgaatcaga ttcgtccgtt tttgcctttg
    11101 ctaatccaat accaagattt cttgagtgat attgacgtgg tggctgccaa agcaaaatat
    11161 gccaatcgaa tcaatggaat tttgccaaca attaccgaaa atagacgatt gttttttagg
    11221 gatgctttcc atcctatttt gtatttgaac aacaaacaga aaaacgaaat cacgcatccg
    11281 caaaccatcg aattgagtca agaaaaccgc atcatcgtaa tttctggacc taatgctggg
    11341 ggaaaaacca tatctctaaa aactgttgga ttattgcaat tgatgttgca atctgggatg
    11401 ttgattcccg tgcacgaacg cagtgaaacc tttttattcg atagaatcct aaccgatatt
    11461 ggcgacaatc aatctattga aaatcatttg agtacctaca gttaccgatt gaagaatatg
    11521 aactacttct tgaagaaatg caacaagaaa acaatgttct tgattgatga attcggaacg
    11581 ggttctgatc cagaattagg tggtgctttg gctgaaattt tcttggaaga attctaccat
    11641 cgggaagctt ttggaattat taccacgcat tattcgaacc taaaaattct ggcgaacgaa
    11701 ttgccttttg ccaccaatgc caatatgtta ttcgacgaaa aaacgttaga accaatgtat
    11761 aaattggttt tgggacaagc cggaagttct ttcacctttg aagtcgcctt gaaaaacgga
    11821 attccgtatg gattaatcaa tcgggcgaaa aagaaaatcg aagtgggaaa agtgcgattt
    11881 gacaaaacca ttgccacgct ccaaaaagaa cgttccaaat tggaaaaaac ttccatcaat
    11941 ttgaaagaag aagaaactcg agctcgtgag gaaggcaaga aaatggaaaa catcaacctg
    12001 aaaataaagc aaaaactgga aagctatcag gaattgtatg acagcaacca aaaaaccatt
    12061 tacatcggcc agaaaataga agatattgcc gagaaatatt tcaacaacaa aaacaagaaa
    12121 gacttgattg gcgaatttct aaaattggtc gaaatcgaaa attcgaaacg caaaaaaacg
    12181 acgccaaaag tggccaaagc cattatcgaa aagaaaaaag aaatcatcaa ggaagtcgaa
    12241 atcaaggtcg aagaaattag aatcgagaaa aaggaaaaga aactcaaacc tgttatcgaa
    12301 aaaccaaaac ccatcctaaa agtaggcgac cgagtgagaa tgatggacgg aaaagcggtg
    12361 ggaagcattg acaaaatcga gaaaaacaaa gctgtggtta attatggctt attcacttcg
    12421 aaagtgagtt tggaggaatt ggaattggtg gaagcggtga aaaaataaga gtcattgagt
    12481 taaaaaatgg aacacggatg acacagatgc aacggataaa atacggattt tataaatcta
    12541 tttttatata aaaacctgtg taaatcagtc caatccgtaa aatctgtgag ctaatttttt
    12601 attttactag gctcctagca ataacataat taacaaacag aatgcaaaac ttacccaaaa
    12661 acaaaaaaat aattctcttt gacggcgttt gcaatttgtg caattcggcg gtgcaattcg
    12721 tgattcggca cgacaagaaa gacgtgtttc tattcgtggc tttgcaatcg gaattgggtc
    12781 aagatatttt gaatcatatt ggaattaatc caaaaaacat cgacagcatt atcctgtatg
    12841 aaccaggaat tgcttattat tataaatcac aagctgctat tgaaattgcc cgaaatttgg
    12901 gtggattttg gcattttgga actattttca gaattattcc aaccccaatt gccaatcagc
    12961 tttatgatta cgttgccaag aatagatata aatggtacgg caaaaaagaa agttgtatga
    13021 tgccaactcc ggaactaaaa tcaaaatttt tataaaaaat ggccaactat gatatttatc
    13081 atattaaatg attgacaata cctgtaaatt tgaccaatct taataataaa taattatggg
    13141 aaatatacca ctatattcat ggggaaatgg gtcatttata atgatttttg tttttggttt
    13201 agtaataatt ggtttaattg cagctgtttt ctttatgatg aattccgata agaaaaaaaa
    13261 ataagattat aggattatta aaaaaagagg ataactaatt agttatcctc tttttatttt
    13321 caaaaaaaac cagttcttaa cttcgaatta attttctgta tttcaaacgt tttggagtta
    13381 aatcaccacc taaacgtttc tttttgtttt cttcataatc agaaaaacca ccttcaaaat
    13441 aatacacttc tgaattacct tcgaaggcta gaatatgagt acaaattcga tccaagaacc
    13501 atctgtcgtg cgaaatcact acagcacatc cagcaaaatt ttctaaacct tcttctaatg
    13561 ctcgaagggt gttcacgtcc aaatcatttg ttggctcatc taataaaaga acgtttcctt
    13621 cttcttttaa tgtcattgct aagtgcaaac ggtttctttc tccacccgaa agcatcgata
    13681 ctttcttgtt ttgttctccg ccaccgaagt taaaccttga caaataagcg cgagaattta
    13741 cttgttttcc gcccatcatc accaattcct gtccgtcagc aaagttttcc caaatggatt
    13801 tgtttggatc aatattagaa tgcgcttgat ctacgtaagc aattttcaca gtatcaccaa
    13861 ttaaaaattc acctccatct ggtttttcct cacccataat cattttgaaa atggtagatt
    13921 taccagctcc gtttggtcca ataattccaa caattccagc ctgtggcaag gtaaaattta
    13981 agttatcata caataatttg tctccaaaag ctttggctac acctttagcc tcaataacat
    14041 tagttcccaa tcttggcccg tttggaatgt agatttccaa gttttcgtcc aattgttttt
    14101 ggtcttcatt caacaattta tcgtaatttt gtaaacgcgc tttttgctta gtttgacgcc
    14161 ctttcgctcc ttgacgaacc cattctaact cgcgttccag agtttttcta cgtttagaag
    14221 ctactttttc ttcctgagcc atacggtttg atttttggtc tagccaagac gaataatttc
    14281 ctttccaagg aataccttct cctctatcca attccaaaat ccaaccggca acattatcca
    14341 agaagtacct atcgtgagtt actgcaatta cagttccaga atattgtgcc aaatgttgtt
    14401 ctaaccaaag cacgctttct gcatccaagt gattcgttgg ttcatccaaa agtaaaacat
    14461 caggttgttg caataacaaa cgacataaag ctacacgacg acgctcacca ccagataaat
    14521 tcttgattgg ggtatcaccg tcaggagtac gcaacgcatc cattgctatt tctagtttgg
    14581 tatcgatttc ccaagcacca agtgcgtcga ttttgtcttg taaagcggct tgacgatcca
    14641 tcaatttatc cattttgtct ggatcagaat aattttcttc aagaccaaat aaatcgttga
    14701 ttttattgta ttcatccaaa acagccatcg tttcggcaac tccttcctga acaatttcga
    14761 ggacagtttt tgaatcgtct aactgtggat cttgttctaa ataacccacg gtataacctg
    14821 gttgaaaaac aacatcgcct tgataatttt tatcaactcc cgcaataatt ttcaaaaggg
    14881 aagattttcc ggtaccatta agtcccaaaa ttccaatttt agctc
//
LOCUS       DAOSNN010000031        14785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_4086_length_14785_cov_5.412627, whole genome shotgun sequence.
ACCESSION   DAOSNN010000031 DAOSNN010000000
VERSION     DAOSNN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14785
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4086_length_14785_cov_5.412627"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PLQ70_02935"
     CDS             complement(<1..892)
                     /locus_tag="PLQ70_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HQE33598.1"
                     /translation="MFSIFSCKQTESESITPEPEAHQRTEQFGFNFNDFNVLHDTIKK
                     GDTFGSIIEHQNLGGKEVYDIIQKVKDSFDVRTVRIGKPYTFLRSKDRYKKLQYAIYQ
                     PDRASYFIIDFRDSVSVSKVTRPIRIKKRTIAGELKGSFSEELHRQKVDPALANKLIK
                     VYAWSIDFFKLKKGDQFGVIFTERYIDDTIYDGVDSLKAAFFEYKGKMVYAFPFAQNK
                     GGKIDYYDEDGKALKNFFLKSPLKYANITSRFSRSRFHPVQMIWKAHKGTDYAAPTGT
                     PIMTTASGIVEQTGYTAGNGN"
     gene            complement(992..1774)
                     /locus_tag="PLQ70_02940"
     CDS             complement(992..1774)
                     /locus_tag="PLQ70_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQE33599.1"
                     /translation="MKKIILVLIGFAVLGFTPQKESAFDVGEQFVFRVHYQFVQAGYA
                     TLEIKDATINKKKVFKVIGKGYTTGVSRFFFKVDDTFESYIDKETGSPYQYVRKTDEG
                     GYTKNQEGFFNPSENKVLVKDYKHNTEKTFSIPKNTQDILSSFYYLRNYPNIDKMKVG
                     EFVAIDMFFDDKTTKFRLKFIGREDIRTKFGIVSTMIFRPLVQTGRVFKEEESLTVWI
                     SDDDNKLPVRIKASLLVGSIKADLDSYKGLKFPLKVKKNKRS"
     gene            complement(2107..4371)
                     /locus_tag="PLQ70_02945"
     CDS             complement(2107..4371)
                     /locus_tag="PLQ70_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQE33600.1"
                     /translation="MRIKAIGNRQKPRSKKSTILPIAYCLLPFIFCLLPIASQAQDTI
                     KRPKIGLVLSGGGAKGFAHVGVLKVLEQAGVKIDFIGGTSMGAVIGGLYASGYNATQL
                     DSIVKVTNFDNLLIDYIPRSSKSFYEKRNDELYALILPFNKFKIGAPQSLSKGMFNYN
                     LFNRLTLHARHVRDFNQLPIPFLCMATDIELGKQVVLDKGVLAQALFASSALPSVFSP
                     VILDGKLLVDGGVTNNYPIDEIRKLGADIIIGVDVQSGLRGKEELQDGTKILFQITNF
                     QMIEKMNANSKNTNIYIKPDITNIGIVSFEKAKEIILRGEEAAFTVYEDLDKLADKSN
                     PYLKPKLKIASDSLQIVNIICHNLVHYSKAYIEGKLKFKSGAKINYKDLETGINNIDA
                     THNFGTVTYSLEPNGSGDDLVLNLIENPIITYLKFGLHYDGLFKSAVLANITNKNTFF
                     KNDVASLDLIIGDNVRYNLDYYIDNGYNLSIGFKSQLNQFNKNVTKQISSLSIDPSVN
                     SINVNFIDLTNQLYFQSIFAQKFLIGLGLEHKYLNINSETIVNSNSVIDRSKYGSVYA
                     YMKYDSFNNQYFPTKGWYLMGDIHNYLFSTDYSGKFNPFSIAKADAGIAVKVFNKTTF
                     KLQTEGGFSIGEKSVPYFDFVLGGYGFIPVNNFKPFYGYDFLSVAGDSYLKSSLAIDY
                     EVFKKNHLNFSANYANIGTNLFDSLDWISLPKYSGYAIGYGLETIIGPIEIKYTWSPE
                     ITKGFTWFSIGFGF"
     gene            complement(4373..6169)
                     /gene="uvrC"
                     /locus_tag="PLQ70_02950"
     CDS             complement(4373..6169)
                     /gene="uvrC"
                     /locus_tag="PLQ70_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQE33601.1"
                     /translation="MNNPTLELQIQTLPDGPGVYQYYDKEGKILYVGKAKNLKKRVSS
                     YFNKIHDTAKTNVLVKKIVTIKHIVVPTETDALLLENNLIKTLQPRYNVLLRDDKSYP
                     WICIKKEPFSRIFSTRRMVKDGSEYFGPYTSFKTVSTILELIKELYPIRTCNYDLSRS
                     NIENGKFKVCLEYHIGNCKGPCEGYESLEEYQKQVDAIREILKGNFKESMKDFKKLMT
                     DLAQNLHFEEAQKIKEKIEILENYQSRSTIINPKITNIDVFSIISDESAAFVNFLQIS
                     HGSIIRSHTMEIKKKLDETDEELLELAIIELRERFQLLSKEIIVPFEVDLGEKIKVSV
                     PQLGDKKQILDLSIRNAKFYRIEQLKQLQIVDPDRHANRIMAQMQKDLRLPVEPRHIE
                     CFDNSNIQGTNPVSACVVFKDGKPSKKDYRHFNIKTVEGPDDFASMTEVVYRRYKRLL
                     DENEPLPQLIIIDGGKGQLSSALKSIDELGLRGKIAIIGIAKRLEELFYPGDSIPLYL
                     DKKSETLKVIQQLRNEAHRFGITHHRDKRSKSALNSSIESIPGIGEKTMLALIQHFKS
                     VKRLKLATEKEISDVVGVSKAKKITDFYSKNS"
     gene            6265..7503
                     /locus_tag="PLQ70_02955"
     CDS             6265..7503
                     /locus_tag="PLQ70_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HQE33602.1"
                     /translation="MIYWKKLSQEERKDRIQSALKENVNFSNDASLGYPASKLDGKVF
                     YDDAPFLKDAPTLQTYVANPNHIGCHTLGTSEKAFKGTQEIEREVLNVIAVDIFKAEP
                     NSFDGYISPGGTEANIQAIWMYRNFFMYRKKAKLDEIVILASEDTHYSIPKGANLLML
                     DLIKIPVAFENREIDKIALENEIIKAKQNGKKYFIAIANMGTTMFGSVDNTDDYTSVL
                     EKHNAEYKLHIDGAYGGFVYPFSNQKSTMNFSNPKISSITIDAHKMLQSPYGTGVFVC
                     RKGLIENVLTKEAQYVEGMDLTLCGSRSGANAVAVWMILFTYGPFGWFEKVSILQMRT
                     QFLCHELDELNIQYFREPFMNIVTIHSQYISEEIAKKYDLVPEQHNQDNKWYKVVLMD
                     HVEVQHLTSFINDLKTMIHA"
     gene            7496..8059
                     /locus_tag="PLQ70_02960"
     CDS             7496..8059
                     /locus_tag="PLQ70_02960"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HQE33603.1"
                     /translation="MLKKELRQKYKALRNQLSEEDLEEMSLAIANKILKLPIWEKTYF
                     HIFLPITEHKEVNTEYILHLLSGKDKEIIISKSDFETRVMTHFLLTDNTKIKKNQYNI
                     PEPVDGLEVPSKKIEVVFVPLLAFDKTGHRAGYGKGFYDKFLSECKVETIKIGLSFFE
                     AEETIDDVFENDIKLDYCVSPNEVFKF"
     gene            8351..9346
                     /locus_tag="PLQ70_02965"
     CDS             8351..9346
                     /locus_tag="PLQ70_02965"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQE33604.1"
                     /translation="MTENLKFAVIGGGSWATAIAKMLCVNLPEIAWYMRNEDAIEHIK
                     KHHHNPNYLSSVEFDIKKLKLTSDINEAVGYADYIIFAIPSAFLNGELEKLTESLEGK
                     VVFSAIKGIVPETSLIVGEHFNTTYNIPFDNIGVITGPCHAEEVALERLSYLTIACGD
                     AKKAKIMAKNLSGNYIKTKISDDIIGTEYAAMLKNIYAIAAGIAHGLGYGDNFQSVLM
                     SNGIREMKKFIKKVHKMKRNINDSAYLGDLLVTGYSIFSRNRMFGNMIGKGYTVKSAM
                     MEMSMVAEGYYAAKSAYNLNLDYGAQTPIINAVYSILYEGKSAKSVFKKLTDKLD"
     gene            complement(9343..11688)
                     /locus_tag="PLQ70_02970"
     CDS             complement(9343..11688)
                     /locus_tag="PLQ70_02970"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HQE33605.1"
                     /translation="MKKSNSRYFYISALRNYKHVWVSLFILLVGLSLTFTVYKYVRQE
                     EETQSKKEFDQVCNEIKSKIITQINMHSEFLISGSSLFEASESVTREEWKNFNKFSSE
                     NVKFMGVQSLGFATIIKKNQLKAHISTIRKEGFSEYNVFPNQARDLFTGIIYLEPFTK
                     KNASVLGYDMFSESVRREAMQMARDNDKPTLTGKVKLRIETKKNWQAGTILFVPVYKN
                     LLTNTVEARRKAIIGWVFCAFRMDDLMNSFLASKYFNEQTHIRLQIYDEGIISQKALL
                     FDSQKNLTRNNANEESRTRILPVVFNNKKWTLVFSRPTEGMFFFSSQTLFILIGGTTI
                     SLLMFALSYSLLITKKKAKIMAEQLSADLLNKNLEYELMNKSLNKNYKRLITSKEKLK
                     AINIELQKAKIKAEESDKLKSAFLANMSHEIRTPMNGIMGFAELLKQGNLSLEEQKKY
                     IEIIEKSGLRLLSIINDIVDVSKIEAGQMNVSLSTTNIDEQLQYINTFFNPEAQDKGI
                     QLILKKNTGVKASTITTDREKLYAILINLVKNAIKYTIKGTIEFGYEIQGENILFFVK
                     DTGIGISKDRQKAVFERFIQADFNDKMARQGAGLGLAIAKAYVDLMGGKIWVESEPDK
                     GSTFYFTLPYTISNQEIQNSTNGLEVNSAPIEVKNLKILVTEDDNISRILILKVLEEF
                     SSEILTARTGLEAVEICRNNPDIDLILMDMQMPLMNGYEATKEIRKFNKEVIILAQTA
                     FALEGDKSKTIDAGCNDYISKPIKKQELSVLLQKYFDHISY"
     gene            12009..12680
                     /locus_tag="PLQ70_02975"
     CDS             12009..12680
                     /locus_tag="PLQ70_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQE33606.1"
                     /translation="MKAFLYQYRKFFGVLLVFSIIVISLFYSALKPKKSLPIYNPSDV
                     NPELVDTTVQYISKYHKIADFSFTNQNGKTITQKDYEGKIYVADFFFTTCKSICPKMT
                     TNLVDVQKAFLDNPKVKLLSFTVMPDIDSVSVLKDYAQINGVIDTKWNLVTGDKKAIY
                     TMARKSYLAVKQGKPEELYDMVHTENFVLVDSKKRVRGFYDGTKKEEIQKLIEDINWL
                     LEDEN"
     gene            12884..13117
                     /locus_tag="PLQ70_02980"
     CDS             12884..13117
                     /locus_tag="PLQ70_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HQE33607.1"
                     /translation="MRTTIANLKKGEKAIIKDFDADVIPLKLLEMGCLPGNTVELLQI
                     APFGDPLYLNINGSHLAIRIETAREIEIDLIKK"
     gene            13124..>14785
                     /gene="feoB"
                     /locus_tag="PLQ70_02985"
     CDS             13124..>14785
                     /gene="feoB"
                     /locus_tag="PLQ70_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQE33608.1"
                     /translation="MSKQNINVALIGNPNTGKTSVFNQLTGLNQQVGNYPGITVEKKV
                     GFCKLPNNINANVIDLPGTYSLNASSADENVVIELLLNKKDKLFPDVIVVVSEVENLK
                     RNLLLFTQIKDLEIPTILVINMIDRMQLKGISLDIPFLEEQLKTKIALVSSRKNIGIS
                     ELKTLITTYKTISTEPCLNASSIDDEYFNSLRKTFPNQSLYKLWLVITQDVNFADLNR
                     KTLEIHSFTKTKSELKKLQQQETIKRYQFINNTLKIGRKVDSASATDFRSQLDRVLTH
                     KFWGYLIFFVVLFGIFQSIFEWSKIPMDLIDSSFAFLSTAASENLPPGAFTNLIAQGI
                     IPGIGGILMFIPQITFLFLFISILEESGYMSRVVFLMDKIMRRFGLSGKSVVPLISGT
                     ACAIPAIMATRNIENWKERLITILVTPFTTCSARLPVYAIIIGLVIPDKRVFGVVNMQ
                     GLTLMILYLIGFGMAIISAFILNKVLKLQCKTFFVVEMPNYKLPMFKNVVINVVEKTK
                     AFVFGAGKIILAISVILWFLASYGPGQKFNNAETILAAQNKNTTFS"
BASE COUNT         4799 a         2574 c         2377 g         5035 t
ORIGIN      
        1 aatttccgtt tccggcggta tagcccgttt gttccacaat tcctgatgcc gtggtcataa
       61 ttggagttcc tgttggcgca gcataatcgg ttcctttgtg ggctttccag atcatttgta
      121 cgggatgaaa tctacttctc gaaaaacgcg aagtaatgtt agcgtatttg agtggcgatt
      181 tgaggaaaaa attcttgagc gcctttccgt cttcgtcata ataatcaatt tttcctcctt
      241 tattttgggc aaatggaaag gcataaacca tcttgccttt atattcaaaa aaagctgctt
      301 tcaaactatc aacgccatca taaatagtgt cgtctatata tctttcagta aaaataactc
      361 caaattgatc accctttttt aacttgaaaa aatcgattga ccaagcatat acttttatta
      421 atttattggc caatgctgga tctacttttt gcctatgtaa ttcttctgaa aacgaacctt
      481 ttaattctcc agcaatggtt cgttttttaa ttcgaatggg tcttgttact ttagaaacac
      541 taaccgaatc tctaaaatca ataataaaat aactagctct gtcaggctga taaattgcat
      601 attgtagttt tttgtaacgg tcttttgagc gcaaaaaagt ataaggtttc ccgattctaa
      661 cggttctaac atcaaaacta tcttttactt tctgaatgat gtcataaact tctttaccgc
      721 caagattttg gtgctcgatg atactcccaa aagtgtcccc tttttttatg gtgtcgtgaa
      781 gaacattaaa atcgttgaaa ttaaaaccaa attgttctgt tctttgatgt gcctcaggct
      841 ctggagtgat gctctcagat tctgtttgtt tacaagaaaa tattgaaaac aaaacaataa
      901 taattagaaa tacttttttc aaattaacta taattattca tttatttatc aagcggtctt
      961 tttcgtaatc aattaaaatt tgagtcaaac atcaactacg cttgtttttt ttaactttta
     1021 agggaaattt taaccctttg taactatcta aatctgcttt tatagagcct acaagaaggc
     1081 ttgcttttat tcgaacaggc agtttattgt cgtcgtctga tatccaaacg gttaaacttt
     1141 cttcttcttt aaatacgcgt ccggtttgaa ctaatggtct aaatatcatt gtcgaaacga
     1201 ttccaaattt agttctaata tcctcacgtc ctataaactt taacctaaat tttgtagttt
     1261 tatcgtcaaa aaacatatcg attgccacaa attctcctac tttcatttta tcgatattgg
     1321 gataatttcg cagataataa aaagacgata aaatatcttg ggtgtttttg ggaatagaaa
     1381 aggtcttctc ggtattgtgt ttgtaatctt ttactaaaac cttattctca gatgggttga
     1441 agaatccttc ttgatttttg gtataaccac cttcatcagt ttttcgaaca tattgatacg
     1501 ggctacctgt ttctttatca atataacttt cgaaggtgtc atctacttta aaaaaaaatc
     1561 gagaaactcc agtcgtatag ccttttccaa taactttaaa aacttttttt ttatttattg
     1621 tggcatcttt aatttctaaa gtggcgtaac ctgcttgtac aaactggtaa tggactctaa
     1681 aaacaaattg ttctcctaca tcaaaagccg attccttttg aggtgtaaat cctaagacag
     1741 caaaacctat aagtactaga ataatttttt tcatcctttt ttaaactatt gttttactgc
     1801 taaacaaatt ttgttccaaa tgtattaaaa caaaaaaact cagtcaaata atgactgaga
     1861 attcatgcta ttaactaacc aaaaaaatat aaattgtgaa aatttatatg aaatgcgaaa
     1921 ggaaaccacc cctttcgttt tttcaggtgc aaaggtacaa ctggttttag ataaaattta
     1981 ataaaaataa agtttaacat attgaatgca aacacttaaa ttgatttcaa cttatttttt
     2041 tacattatat aaaaaaaagt gggtttttct gttgtctcta taaattttat tttggctgtt
     2101 tgcgatttaa aacccaaatc caatgctaaa ccaagtaaat cctttggtga tttctggcga
     2161 ccaagtgtat tttatttcta ttggaccaat tatggtttct aatccataac ctattgcata
     2221 tcccgaatat tttggcaaag atatccagtc taacgaatca aaaagattgg ttccgatatt
     2281 ggcataatta gctgaaaaat tcaaatggtt tttcttgaaa acctcataat caattgctaa
     2341 agaagatttt agataactat ctcctgcaac actcaagaaa tcatatccat aaaaaggctt
     2401 gaaattatta accggaataa aaccgtagcc tcccaaaaca aaatcaaaat aaggaacgct
     2461 tttttctcct atagaaaaac ctccttctgt ttgcaatttg aaggtggttt tattaaaaac
     2521 ttttactgca ataccagcat cggctttggc tatcgaaaat ggattgaatt ttcctgaata
     2581 atctgtagaa aataaatagt tatgaatatc acccataaga taccatcctt ttgttggaaa
     2641 atattgattg ttaaacgaat catatttcat ataagcataa acgctaccat atttacttcg
     2701 gtctataacg gaatttgagt tgacaattgt ttcagaattt atatttaaat atttgtgttc
     2761 taaacctaag cctataagga atttttgagc aaatatggat tgaaaataca actgattagt
     2821 taaatcaata aaattaacat ttatagaatt taccgatgga tcaatagaca aactactgat
     2881 ttgctttgta acattcttgt tgaattgatt caattgcgat ttaaatccaa tactcaaatt
     2941 gtatccatta tcgatataat agtctaaatt atagcgcacg ttgtcgccta taattaaatc
     3001 gagtgatgct acatcgtttt taaaaaaagt gtttttatta gtaatattgg ccaaaacggc
     3061 acttttaaag agtccgtcat aatgcaatcc aaatttaaga taggtaataa ttggattttc
     3121 aattaaatta agaactaaat cgtctcctga accattaggt tctagggaat aagttaccgt
     3181 tccaaaattg tgagtggcat caatattatt gattcctgtt tctagatctt tataattgat
     3241 ttttgcccca gacttaaatt tcaactttcc ttcgatataa gctttggaat aatgaactaa
     3301 attatgacat attatattta caatttgcaa actatcggat gctattttta atttgggttt
     3361 tagataagga tttgatttat ctgctaattt atctaaatct tcataaaccg taaaagcagc
     3421 ttcttctcct ctaagaataa tttcttttgc tttttcaaaa gagacaattc caatgtttgt
     3481 aatgtcaggt ttaatataaa tattcgtgtt ttttgaattg gcattcattt tttcaatcat
     3541 ctgaaaatta gtgatttgaa ataaaatttt agtaccatcc tgaagctctt ctttgcctct
     3601 taacccactt tgcacatcta cacctataat gatgtcggct cctagttttc taatttcatc
     3661 tattggataa ttattagtaa cccctccatc taccaagagt tttccgtcaa gaattacggg
     3721 cgaaaaaacc gatggcaatg ctgaactggc aaataaagct tgtgccaaaa ctcctttgtc
     3781 taaaaccact tgttttccta gttcaatatc agttgccata cacaaaaaag gaattggcaa
     3841 ttgattaaaa tccctcacat gtctggcatg taaagtaagt cggttaaaca aattgtaatt
     3901 aaacattccc tttgaaagag attgaggcgc tcctattttg aatttattaa acgggagaat
     3961 tagggcatac aactcatcat tccttttttc gtaaaaactt ttagaggaac gtggaatata
     4021 atcaattagc aaattatcga aattggttac cttaacaatt gaatctaatt gtgttgcgtt
     4081 ataacccgaa gcataaagtc ctccaattac ggctcccata ctggttccgc caatgaaatc
     4141 tatttttacg cccgcttgtt ctagcacttt caaaactcct acgtgagcaa atcctttggc
     4201 tccaccgccg ctcaaaacta atccaatttt tggtcttttg atggtatctt gagcttgcga
     4261 ggcaataggc aataggcaaa agataaaagg caataggcaa taggcaatag gcaaaatcgt
     4321 actctttttg cttcttggct tttgcctatt gcctattgcc ttaattctca tattaactat
     4381 ttttactgta aaagtcggta atttttttgg cttttgatac acctacaacg tcagagattt
     4441 ctttttctgt tgctaatttc aatcttttaa cacttttgaa atgttgaatc aaggctaaca
     4501 tggtcttttc gccaattcct ggaatgcttt ctatagaaga attcaatgcc gatttgcttc
     4561 ttttgtcccg atgatgggtg attccaaaac ggtgtgcttc gttgcgcaat tgctgaatta
     4621 ctttcaacgt ttccgatttt ttatccaaat acaacggaat ggaatcacct ggataaaaca
     4681 attcttccaa tcgtttggca ataccaataa tcgcaatttt tccacgcaat cccaattcgt
     4741 caatactttt taacgccgaa gacaattgcc cttttcctcc gtcaatgatg atgagttgtg
     4801 gcaaaggttc gttttcgtcc aacaatcttt tgtaccttct ataaactact tcggtcatcg
     4861 aagcaaaatc atctgggcct tcgacggttt ttatattgaa atgacgataa tcttttttgc
     4921 taggttttcc atctttaaaa actacacaag ccgacacagg attcgttcct tgaatattcg
     4981 aattatcaaa acattcgata tgtcttggct caactggcaa tcgcaaatcc ttttgcattt
     5041 gtgccataat tcgattggcg tgacggtctg gatctacgat ttgaagttgc ttcaattgtt
     5101 cgatgcggta aaacttggca ttgcgaatgg acaaatccaa aatctgtttt ttatcaccca
     5161 attgcggaac gctcactttt attttttcgc ccaaatctac ttcaaacgga acaataattt
     5221 ctttggataa taactgaaaa cgctcccgaa gttcgataat tgccaattct aataattctt
     5281 cgtcggtttc gtccaatttc tttttgattt ccattgtatg cgaacgaata atcgacccgt
     5341 gtgaaatttg caagaagttg acaaaagcag cactttcgtc cgaaattatc gaaaacacat
     5401 caatattggt gattttcgga ttgataatcg tggatcggga ttggtaattt tcgagaattt
     5461 caattttttc ttttattttt tgggcttctt caaaatgaag attttgagcc aaatcagtca
     5521 tcagtttttt gaaatccttc attgattctt tgaaatttcc tttcaggatt tcccgaatgg
     5581 catcgacttg tttttggtac tcttccaaag attcatagcc ttcgcaaggc cctttgcaat
     5641 tgccaatatg atactccaaa caaactttaa acttgccgtt ttcgatatta gaacgagaca
     5701 aatcataatt gcaagttcgg attggataca attcttttat caattctaaa atagtgctta
     5761 ctgtcttgaa actcgtgtaa ggtccaaaat attctgaacc atccttgacc attcttctgg
     5821 tagaaaatat tctagagaaa ggttcttttt tgatgcaaat ccaaggataa cttttatcat
     5881 cacgcaacaa cacattatag cggggttgca aggtttttat aagattgttt tctaataaaa
     5941 gtgcatcagt ttcagtagga accacaatgt gtttgatggt tacaattttc ttgaccaaga
     6001 cattggtttt ggccgtgtcg tgaattttat tgaaatagga agaaactctt tttttaaggt
     6061 ttttggcttt gcccacatac aaaatcttcc cttccttatc ataatattga taaactccag
     6121 ggccatcagg caaggtttgg atttgaagtt cgagagttgg gttgttcata taacaaagtt
     6181 agcctgaaaa atatgaagtt ccaaattaga acaactagct aatattctga aaagttatag
     6241 taactttatc aaaaatattt agcaatgatt tactggaaga aactctctca agaagaaaga
     6301 aaagacagaa tacaaagcgc tttgaaagaa aatgtaaatt tttctaacga tgcttcttta
     6361 ggttatcccg cttcaaaact ggatggaaaa gtattttatg acgatgcccc tttcttgaaa
     6421 gacgcaccaa ctttgcaaac ctatgtggcc aatccgaatc acattggctg tcatactttg
     6481 ggaacttccg agaaagcttt taaaggaaca caggaaatag aacgcgaagt tttaaacgta
     6541 attgccgtcg atatttttaa agcagaacca aattcgtttg acggatatat ttctcccgga
     6601 ggaaccgaag ctaacattca ggccatttgg atgtatcgca acttttttat gtatcgaaaa
     6661 aaggcaaaat tggacgaaat tgtcatttta gcttcagaag acacccatta ttccattcca
     6721 aaaggagcca atcttttgat gctagattta atcaaaattc cagttgcttt cgaaaacaga
     6781 gaaattgaca aaatagcttt ggaaaatgaa atcatcaaag ccaaacaaaa cggtaaaaaa
     6841 tattttatcg ccattgccaa tatgggaaca acaatgtttg gttctgttga caataccgac
     6901 gattatactt cggttttaga gaaacacaat gccgaataca aactccatat tgacggggct
     6961 tatggcggtt ttgtatatcc gtttagcaat caaaaatcga ctatgaattt tagcaatccg
     7021 aagattagtt ccattaccat tgacgctcac aaaatgctgc aatcaccata tggaactggt
     7081 gtttttgttt gtcgaaaagg gctaatagaa aatgttttga ccaaagaagc ccaatatgtc
     7141 gaaggaatgg atttgaccct ttgcggaagt cgttcaggtg ctaatgcggt ggcagtttgg
     7201 atgattttat ttacttacgg cccgtttggt tggttcgaaa aagtaagtat tttgcaaatg
     7261 cgaacccagt ttttgtgtca cgaattagac gaattgaaca tccaatattt cagagagcct
     7321 tttatgaata ttgtcactat tcattcgcaa tatattagtg aggaaattgc caaaaaatat
     7381 gatttggtgc ccgaacaaca caatcaagat aataaatggt acaaagttgt actaatggat
     7441 cacgtagaag tgcaacattt gacttccttt ataaacgatt tgaaaacgat gattcatgct
     7501 taaaaaagaa ctgcgacaaa aatacaaagc cttacgcaac caactttcag aagaagattt
     7561 ggaggaaatg agtttggcta tagccaataa aatactaaag ttgcctattt gggagaaaac
     7621 ctatttccat attttcttgc ccatcacaga acacaaagaa gtcaacaccg aatatatttt
     7681 gcatttactt tcaggaaaag acaaagaaat tatcatttcg aaaagtgatt ttgaaacaag
     7741 agtaatgact cattttcttt tgacggataa taccaaaatc aagaaaaacc aatacaacat
     7801 tccagaaccc gttgacggcc tagaagttcc ttcaaaaaaa atagaggtgg tttttgtacc
     7861 gcttttagct tttgacaaaa ccggacatcg agcgggttat ggcaaaggat tttatgataa
     7921 attcttgagc gaatgcaaag tagaaaccat taaaattggg ctatcctttt ttgaagccga
     7981 agaaactatt gatgatgttt ttgagaatga tataaagttg gattattgtg tatcgccaaa
     8041 tgaagttttc aaattttaat cgaaaataag cacaatttaa aatttttgaa ttagaacaaa
     8101 ataaaaaggc gccctcaaaa cctttaagaa cgccttttgc aaaatactaa aacaaaacta
     8161 actaattcaa cctcaataat ttaaaaataa ccaattttta aacaaccata acgcataaaa
     8221 attttaaata ttgtgttgaa ataaaaaaaa gttgaaaatt atttttttga ttttaaaatt
     8281 ccttttctct ggcttacttt tgcttcataa gtatattttt aagtactttt gattaaattt
     8341 aataaataaa atgaccgaaa atttaaaatt tgccgtaatt ggtggaggaa gttgggcaac
     8401 agcaattgct aaaatgttat gcgtaaacct acctgaaata gcttggtata tgcgaaatga
     8461 agatgcaatt gaacacatca aaaaacacca tcacaaccca aattatctaa gctcagtcga
     8521 atttgacatt aaaaaactaa aactaaccag cgacatcaat gaggccgttg gctatgctga
     8581 ttatattatt tttgccattc cctcggcttt tttgaatgga gaactagaaa aacttaccga
     8641 aagtttggaa ggcaaagttg ttttttcggc catcaaagga attgttcctg aaacgagttt
     8701 aattgttggc gaacatttta atacaaccta caatattcca ttcgataata ttggtgtaat
     8761 tacagggcct tgccacgccg aggaagttgc gctagaacgt ttgtcctatc ttacaatagc
     8821 ttgtggcgat gccaaaaaag ccaaaataat ggccaagaat ttatctggaa attacatcaa
     8881 aaccaaaatc tctgacgata ttattggcac cgaatatgcc gcaatgctca agaatattta
     8941 tgctattgca gcaggaattg cccacggatt gggctatggc gacaattttc aatcggtttt
     9001 gatgagtaac ggtattcgtg agatgaaaaa attcatcaaa aaagtacaca aaatgaaacg
     9061 aaacatcaat gattcggctt atttgggcga tttattggta accggatatt ccattttctc
     9121 tagaaataga atgttcggaa atatgattgg aaaaggctat accgtaaaat cagctatgat
     9181 ggaaatgagt atggttgccg aaggctatta tgcagccaaa agtgcctaca atctcaattt
     9241 agattatggt gctcaaacac ctattatcaa tgccgtttac agcattttat acgaaggaaa
     9301 atcggcaaaa tcagttttta aaaaactaac tgataagtta gattagtaac taatgtggtc
     9361 aaaatacttt tgaagtaaaa cagacaattc ttgtttttta attggtttag aaatatagtc
     9421 gttgcagccc gcatcaattg tttttgattt atctccttct aaagcaaatg cggtttgtgc
     9481 caaaataatt acctctttat taaattttct aatttcttta gtggcttcat atccattcat
     9541 aagtggcatt tgcatgtcca ttaaaatcaa atcgatgtct ggattgttgc gacaaatttc
     9601 taccgcttct agacctgttc gtgccgttag tatttcgcta ctaaattctt caagtacttt
     9661 caaaattaat attctggaaa tattgtcgtc ttcagtaact aatattttta aattttttac
     9721 ttcaattgga gctgaattta cttctagccc attagtagaa ttctggattt cttgattact
     9781 gatggtatag ggaagcgtaa aataaaatgt agagccttta tctggttcac tttctaccca
     9841 aattttgccg cccattaaat ctacataagc tttggctatg gccaaaccta atccagcacc
     9901 ttgacgagcc attttatcat taaaatcggc ttgaataaag cgttcgaata cggctttttg
     9961 tctgtctttc gatattccga ttccggtatc ttttacaaaa aacagaatat tttcaccttg
    10021 tatctcatac ccaaattcta ttgttccttt gatggtatat ttgatggcgt ttttaactaa
    10081 attgattaga atggcataga gtttttctcg atcggtagta attgttgatg cttttacgcc
    10141 agtatttttt ttcaaaataa gttgtattcc tttgtcttgg gcttcgggat tgaaaaacgt
    10201 gttgatgtac tgcaattgtt cgtcgatatt ggtagtcgaa aggctaacgt tcatttgtcc
    10261 agcttcaatt tttgaaacat caactatgtc attgataata ctaagaagtc ggagaccact
    10321 tttttcaatg atttcgatat actttttttg ctcctctaaa cttagatttc cttgtttcag
    10381 caattcggca aagcccatta ttccattcat tggtgttcta atttcgtggc tcatattggc
    10441 caaaaaagcc gattttagtt tgtcgctttc ttctgctttt atctttgctt tttgtaattc
    10501 tatattgatt gcttttaatt tttcttttga tgtaattagc cttttatagt ttttattcag
    10561 cgatttattc attagctcat actccaaatt tttatttaac aaatctgcac ttaattgctc
    10621 ggccataatt tttgcctttt ttttggtaat aagtaaagaa taggacaatg caaacatcaa
    10681 aaggcttatt gttgtaccgc caattaatat aaacagtgtt tgagatgaaa aaaagaacat
    10741 tccttctgta ggtcttgaaa acactaaagt ccatttttta ttattaaaaa ctacaggaag
    10801 aattcgagtt ctactttctt cattagcgtt gtttcttgtc aagttttttt gactgtcaaa
    10861 caagagtgct ttttgtgaaa ttatgccttc atcataaatt tgtaaacgaa tatgggtttg
    10921 ctcattaaaa tacttggatg ccaaaaaact attcattaaa tcatccattc taaaagcaca
    10981 aaaaacccag ccaataatag ctttccttcg agcttcgaca gtatttgtga gcagattttt
    11041 gtaaacggga acaaatagta ttgttcctgc ctgccaattt ttcttagttt ctatgcgaag
    11101 cttgactttt ccggttagtg ttggtttatc attatctcta gccatttgca ttgcttctcg
    11161 tctaacggac tcggaaaaca tatcgtatcc caaaacagag gcattttttt tggtaaaagg
    11221 ctctaagtaa attatacccg taaaaagatc tcttgcttga ttaggaaaaa cattatattc
    11281 tgaaaatcct tctttccgaa tagttgaaat gtgtgctttg agctggtttt ttttaattat
    11341 tgtagcaaat cctaaacttt gaactcccat aaatttcaca ttttcactag aaaatttatt
    11401 gaaattcttc cactcttctc gagttacact ttcagaagct tcaaacagtg acgaaccact
    11461 aattaagaac tctgaatgca tattgatttg ggtaattatt tttgatttaa tctcattaca
    11521 tacttgatca aattcctttt ttgactgagt ttcctcttct tgtctaacat atttataaac
    11581 agtaaaagtg agcgaaagtc caacaagtaa aatgaataga gatacccaaa catgcttata
    11641 atttcttaag gcacttatat aaaaatatct tgaatttgac ttcttcatta tttgctgaat
    11701 tgtaaaacct cttgaagatg cggttttttt atgagataaa tttatgtttt tttttaacac
    11761 ttcattaaat gtgaaagcca ataaaacagc acaaattaag aaaaaaaagc caataaaaca
    11821 atctattaga tgactttatc ttatttgcaa aaagtattta taagcctcta aaattaaaaa
    11881 tcttagtttt ttgattaatt catgatgcct ataacttgaa ggtgccaaat acataagcat
    11941 caaaattcaa caacaaacga agtcaaatat aaaactccat tttcgtattt ttgcgccaaa
    12001 ttattcttat gaaagccttt ctttatcaat atcgaaaatt ctttggagta ttgttggtct
    12061 tttccatcat tgtgatttcc ttgttttatt cagcattgaa acccaaaaaa tcattaccta
    12121 tatacaatcc atcggatgta aatccagaat tggtagatac aactgttcaa tacattagca
    12181 aataccacaa aattgccgat ttttcgttta ccaaccaaaa cggcaaaacc attacccaaa
    12241 aagattatga aggcaagatt tatgtggccg attttttctt caccacctgc aaatctattt
    12301 gcccgaaaat gacaactaat ttggtcgatg ttcaaaaagc atttttagac aatccgaaag
    12361 taaaattgct gtcgtttacg gtaatgccgg acattgacag cgtttcggtt ttgaaagact
    12421 atgcacaaat aaatggtgtg attgacacaa aatggaactt agtaactggc gacaaaaaag
    12481 cgatttatac catggctcga aaatcctatt tggccgtaaa acaaggaaaa cctgaagagc
    12541 tttatgatat ggttcatacc gaaaattttg tgcttgttga ctccaaaaaa cgagttcgtg
    12601 gcttttatga cggaaccaaa aaagaggaaa ttcaaaaact aattgaagat ataaattggc
    12661 ttttggaaga tgaaaattaa ttttttgtta tagattttta ctgaattaca agtgggggtt
    12721 ttaattctta aaccattgta ttcaattgat tttaaaatgg ttaatttcaa aaaatcacta
    12781 caaaaacact taaaataatt cgaaaaattc aaacctgaca attatcatta aatattcttt
    12841 tttttactgt acttttgtaa tctaaaatca atctaaataa gaattgcgaa ctacaatagc
    12901 caacctaaaa aaaggagaga aagccatcat caaagatttt gatgcagatg ttattccgtt
    12961 aaaattatta gaaatgggtt gtttgccagg aaacaccgtg gaattactcc aaattgctcc
    13021 ttttggcgat cctttgtatt taaatatcaa tggttcacac cttgctattc gtatcgaaac
    13081 ggctcgtgaa atcgagattg acctcatcaa aaaataaccc aaaatgagca aacaaaatat
    13141 caatgtcgct ttaattggga atccaaatac aggaaaaact tcggttttca accaacttac
    13201 gggcttgaac caacaagtag gaaattatcc tggaattacg gttgaaaaaa aggttggttt
    13261 ttgcaaatta cccaacaata tcaatgccaa cgtaatcgat ttgccaggaa cctatagttt
    13321 gaatgctagt tctgccgacg aaaatgtggt gattgaacta ttgctcaaca aaaaggacaa
    13381 actctttccc gatgtgattg ttgtcgtttc tgaagtggag aatttgaaac gtaatttgct
    13441 gctttttaca caaattaaag atttagaaat tccaaccatt ttagtcatca atatgattga
    13501 ccgaatgcaa ctcaaaggca tttcgctcga cattcctttt cttgaagaac aactaaaaac
    13561 caaaattgcg ctggtaagtt cccgaaaaaa tattggaatt agtgaactaa aaacgttaat
    13621 cactacttat aaaactattt cgacagagcc ttgtttgaat gcgtcttcta tagatgatga
    13681 atatttcaat tcgcttcgaa aaacgtttcc caatcagtcg ttgtacaaac tttggttggt
    13741 cattactcag gatgtgaatt ttgcggattt aaataggaaa acgctcgaaa ttcattcgtt
    13801 taccaaaacc aaatcggaac tcaaaaaatt acaacaacaa gaaaccatca aacgctacca
    13861 attcatcaat aacacattga aaattggaag aaaagtagat tcggcttcag caacggattt
    13921 tcgaagccaa ctcgatcgcg tgctcacgca caaattttgg ggttatctga ttttctttgt
    13981 ggttttattc ggtatttttc aatctatttt tgaatggtct aaaattccta tggatttaat
    14041 cgatagtagt tttgcctttt tgagtacagc agcaagcgaa aatttacccc caggtgcttt
    14101 tactaatttg attgcgcaag gaattattcc tggaattggc ggaattttaa tgtttattcc
    14161 tcaaatcact tttttattct tgttcatttc aatccttgaa gaaagcggtt atatgagccg
    14221 agtggttttt ttgatggaca aaattatgcg ccgtttcggt ctttcgggca aaagtgttgt
    14281 gcctctaatt tcaggtactg catgtgctat tccggctatc atggcgaccc gaaatatcga
    14341 aaactggaaa gagcgattaa tcaccatttt ggtcactccg tttaccacat gttcggctag
    14401 attacctgtt tatgccatta ttattggact agtaattcca gacaaacgtg tttttggagt
    14461 tgttaatatg caaggactta cgctgatgat tctttaccta ataggttttg gaatggcaat
    14521 tatttctgct tttattttga acaaagtttt gaaacttcaa tgcaaaactt tttttgtagt
    14581 ggaaatgccc aattacaaat taccaatgtt caagaatgtt gtaataaacg tagtagaaaa
    14641 aaccaaagct tttgtctttg gagcggggaa aattattctt gcaatatctg ttattttatg
    14701 gtttttagct tcttacggtc cgggtcaaaa attcaacaat gccgaaacca ttttagctgc
    14761 tcaaaacaaa aatactactt tctca
//
LOCUS       DAOSNN010000032        14690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_4119_length_14690_cov_5.164400, whole genome shotgun sequence.
ACCESSION   DAOSNN010000032 DAOSNN010000000
VERSION     DAOSNN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14690
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4119_length_14690_cov_5.164400"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLQ70_02990"
     CDS             <1..739
                     /locus_tag="PLQ70_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQE33609.1"
                     /translation="LSYNVINDKSDNFQVGAALTYSLSDHKNTFNFQVLNARTKTFQE
                     QYGNTAPPGITQTKTPLSVVANWKGHLFDGKLETTYSFAHFIDAEKANRNYFGLGNKF
                     KTGKLILFYDFQYSKEDLDQKCVITNAVKTKYSYAAQDVSYIDNWIRAEYKIQPKVDI
                     LLSLMTNNSYWNGNPDPNKTNRLLTSYGIIPTIEYTPFLDLNLKFYVGYIAKRYDYSS
                     YAESNLNAKDISTGQLSFGIIAPLLVL"
     gene            complement(763..1887)
                     /locus_tag="PLQ70_02995"
     CDS             complement(763..1887)
                     /locus_tag="PLQ70_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE33610.1"
                     /translation="MKTILFIIQKEFKQIFRNKGMLPVIFVLPLVQLLILSNAASFEI
                     KNIKFSYVDNDHSTASRELISKFEASKYFNVIESFSSKKEADSQMQTGTVDMILEIPN
                     HFERDLITEKATNLSVSINAIDGAAAGVESVYILQIINGFNQKIQTQLSQYNDANEVQ
                     PLNIVTIPSFWYNNTLNYKTFMVPGILVLLVTMLTLFLSSMNIVREKEIGTLEQINVT
                     PIQKYQFIIGKLFPFWVLGLLILTVGLTIAKVVFNVPILGNIGLIYFFTSVYLLVILG
                     IGLIISNHSDTQQQAMFVAWFFTVIFILMSGLFTPIESMPQWAQNITLLNPIRYFVEI
                     IRMVMLKGATFSDISKPFFMIVGYAFALNGIAIWSYKKVN"
     gene            complement(1976..3082)
                     /locus_tag="PLQ70_03000"
     CDS             complement(1976..3082)
                     /locus_tag="PLQ70_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE33611.1"
                     /translation="MKRFIGFVTKEFYHIFRDKRTMFILFGMPIAQIMLFGFAITNEI
                     NNVNIAILDQSKDSETQKIINKISASKYFIINDAISSENQIENVFQKGKVKAVLVFET
                     DFIKNLQTHKQAKVQVITDATDPNMANTIANYVNAILQNYTQEINKNNKPSHLIQTQT
                     QLYYNPELKSVFNFVPGVMTIILMLVSAMMTSISITREKELGTMEVLLVSPIKPFQVI
                     VGKVFPYIFLSIINATIILLLGFFVFKMPIEGNLLLLAFESVLFIITALSLGILISTV
                     SNSQQTAMMLSLMGLMLPVIILSGFIFPISSMPMPLQIISNIVPAKWFIIIIKAILLK
                     GATISVIWKETLILVGMTTFFLAVSIKKYKIRLE"
     gene            complement(3123..3857)
                     /locus_tag="PLQ70_03005"
     CDS             complement(3123..3857)
                     /locus_tag="PLQ70_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE33612.1"
                     /translation="MTKEKIITVQNLIKQFGNFTAVNNISFEVYKGEIFGFLGANGAG
                     KTTAMKMLIGISKPTSGAALVAGFDVKTNTEMVKRSIGYMSQKFSMYDDLTLKENITF
                     FGGIYGLSRSQIKEKTARLVAELGLEDTIDKLVGSLPLGWKQKLSFSVALLHEPKIVF
                     LDEPTGGVDPITRRQFWEMIYAEANKGTTIFVTTHYMDEAEYCDRVSIMVEGSIEALD
                     TPKNLKAKYNVDSMNDVFLKLARNVE"
     gene            complement(4089..4985)
                     /locus_tag="PLQ70_03010"
     CDS             complement(4089..4985)
                     /locus_tag="PLQ70_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE33613.1"
                     /translation="MSITVQNISKSYNKTKAVEAISFEVKEGELFGLIGPDGAGKTTI
                     FRILTTLLLANEGSATVAGFDVVKDYKAIRDSVGYMPGKFSLYQDLSVEENLTFFATI
                     FGTIIEENYDLIRDIYVQIEPFKTRRAGALSGGMKQKLALCCALIHKPKVLFLDEPTT
                     GVDPVSRKEFWEMLKRLQQKGITILVSTPYMNEAAMCDRIALIQKGKILKIDSPENII
                     QKYDKTIYAIRTEKMHQLILDLKHYPSRYSVFAFGEFIHYIDKDENFNTKDLETYLAN
                     KNHQEITIQPAITTIEDVFMDL"
     gene            complement(5074..5952)
                     /locus_tag="PLQ70_03015"
     CDS             complement(5074..5952)
                     /locus_tag="PLQ70_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQE33614.1"
                     /translation="MKNIIPFLILASLFSCNKNNDKADGYGNFEATEVTISAESSGKI
                     DYLKLEEGDVLEPKIQVGLIDTTQLYFNKQQLMASKSTVYSKSANVLSQIKVLQEQLK
                     TAQIEKERIQNMFAENAATKRQVDEIDGKVSVILEQIKSVQTQNAPIVNEVKSIDVQI
                     EKINDQIQKSKIINPIKGTVLSKYAEPNEVTAFGKPIYKIADISEMTLRVYVSETQLA
                     QIKVGQKVNVKIDADAGMKTYPGIISWIASSAEFTPKIVQTKEERVNLVYAVKVKVKN
                     DGILKIGMPAEMWVNE"
     gene            complement(6011..7255)
                     /locus_tag="PLQ70_03020"
     CDS             complement(6011..7255)
                     /locus_tag="PLQ70_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE33615.1"
                     /translation="MKRILITFLLLVVFCVNAQQSLTLEDCYALANTNYPIAKQMDLL
                     QQKSNLEVDALQTGKLPKIDLVAQGSYQNQVTTIPNPILEPLNKDQYRANLDVNQLLY
                     NGGLIDANVQLKEAQTKTQQQQVEVNLYQLKTRINQLYFSILLLQERKELLISKQEQL
                     ISRIKEVKSGIRFGAILPASEKVLEAENLKIKQQLSEILFDKKRLFENLSSLTFTVVP
                     ETTILQKPIILAEENTQNNRPELKYFELQNQQIEASKTVIAKNNLPKINAFGLVGYGN
                     PGLNMVDNSFQPILMVGLRANWNVFDWNKTKAEKDALTVSSKIVETEKETFLLNNSLQ
                     LQEMNNEIKKTEEIIKTDTEIIGLREYVEKSANAQLKNGVITTSDYLVEFTNLYEART
                     NQKVHEIQLALAKANYQVVKGN"
     gene            complement(7252..7845)
                     /locus_tag="PLQ70_03025"
     CDS             complement(7252..7845)
                     /locus_tag="PLQ70_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQE33616.1"
                     /translation="MTTEEKIFNAARIVFQKKGFAGARMQEIADEAGINKAMLHYCFK
                     NKELLFQAVFKNAFGQLAPQINEIFSSNDCVFDKIRKFTHSYISFVIQNPYLPQFVIQ
                     EMNNNPEFVMSFLKNANRPNPTLLIAQIEKEIADGIIKPINPKQLLMNMISMTVFPFA
                     AQMMVKGMLQISDSEFNQMMEERKTSIAEQIINSIKR"
     gene            complement(7928..8521)
                     /locus_tag="PLQ70_03030"
     CDS             complement(7928..8521)
                     /locus_tag="PLQ70_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQE33617.1"
                     /translation="MRNSEKSLDQEYWENQYKTKATGWDLGKVSPPLKEHFEVIENKD
                     SRILIPGCGNSYEAEYLLSQGFTDITVIDIAPTLIESLQGKFENNPSIKIILGDFFEH
                     QGEYDLIIEQTFFCALPPKMRENYVSKIHQLLSSKGKLVGLLFNKTFESGPPFGGSQT
                     EYEQLFVTKFEILRMDLCLNSIIPRANSELFIEFQKK"
     gene            complement(8702..8905)
                     /locus_tag="PLQ70_03035"
     CDS             complement(8702..8905)
                     /locus_tag="PLQ70_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008610618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQE33618.1"
                     /translation="MKKNMGSADRISRILFAIVIGVLYFTKAIEGTAALVLGAFAIIF
                     LLTSLISFCPLYVPLGISTCKKK"
     gene            complement(8932..9129)
                     /locus_tag="PLQ70_03040"
     CDS             complement(8932..9129)
                     /locus_tag="PLQ70_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HQE33619.1"
                     /translation="MTKKALIITGIGVVVGAIAGYLYYFYVGCSSGTCAITSKPLNSS
                     LYGAVVGGLLFNMFVKSSKKS"
     gene            complement(9131..9433)
                     /gene="trxA"
                     /locus_tag="PLQ70_03045"
     CDS             complement(9131..9433)
                     /gene="trxA"
                     /locus_tag="PLQ70_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQE33620.1"
                     /translation="MSTSFDTIIQSDKPVLIDFFATWCGPCKTLAPILKQVKDNLGER
                     ITIIKIDVDKNQQLASKYQVRGVPTMILFQNGKQLWRQSGVMSKEEIVKTIVEKSN"
     gene            complement(9430..9633)
                     /locus_tag="PLQ70_03050"
     CDS             complement(9430..9633)
                     /locus_tag="PLQ70_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33621.1"
                     /translation="MKQLLFSNWHLMRWARLAFALFLFIQAYTTREWFFIAFGLFFLF
                     QAIFNLGCGPNGCTVPKNKYSKK"
     gene            complement(9638..11056)
                     /locus_tag="PLQ70_03055"
     CDS             complement(9638..11056)
                     /locus_tag="PLQ70_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQE33622.1"
                     /translation="MKIKQIYTGCLAQGAYYIESKGEVAIIDPLREVQPYIDKANKNN
                     AKIKYIFETHFHADFVSGHVTLAEKTGAPIIYGPTAETKFKSHIAADGDVFKLGEITL
                     TVLHTPGHTLESVCYLLKDKDGKDYALFSGDTLFLGDVGRPDLAQKFANLNKEQLAGI
                     LYDSLRTKIMNLADDVIVYPAHGAGSACGKNLSTETVGTIGEQKRVNYALRADMTKEE
                     FIQEVTDGLLPPPLYFPLNAQLNREGYENVEDIIRDAIAYDVNTFEMVANKIDTVILD
                     VRHQDEYAKGHVPRSIFIGIDGDFAPWVGALIPDIKQPILLITPLGREEESILRLARV
                     GYDNTLGYLKGGFDTWKNEGLEYDTVNSVSAVMLEEIVEEAKEENEKVIIFDVRKDYE
                     YEKEHIEDVSSTPLDNINDHLAEFPKEEEFYLHCAGGYRSMIAASILKARGYHNLINV
                     AGGYSAIKKTAIKTIYGIHHSL"
     gene            complement(11137..11940)
                     /locus_tag="PLQ70_03060"
     CDS             complement(11137..11940)
                     /locus_tag="PLQ70_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQE33623.1"
                     /translation="MEISQLIGYVLAVFVGMILGMLGSGGSILSVPILVYVMGIEPTL
                     ATAYSLFIIGTTSLVGGIHKAKEKLVDFNKVFLFGVPAVISVFVSRKIIIPNIPDILF
                     STDDFTLSKSILIMVVFAIVMILASGSMIKPFKEKVIFENEKLNYYKLTFMGIFIGLL
                     SGFVGAGGGFLIVPTLLLFAKTPIKMAVGTSLFIVSSQSLIGFTGDVMGNQIIDWELL
                     LFFTAASIVGIFMGNFISKKIKEEKLKIGFGWFVLAMGIFIITRELFWS"
     gene            complement(12004..12633)
                     /locus_tag="PLQ70_03065"
     CDS             complement(12004..12633)
                     /locus_tag="PLQ70_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQE33624.1"
                     /translation="MKELLQQTYGYLFEDKLIEEIVATSLLRDFKEGDILIDFGDSIR
                     KMPLLISGAIKILREDFDEGELLLYFIEKGDTCAMTMACCLGETKSEIRAVAETKGQL
                     IMIPVNKMEEWLGKYSSWRNYVFNSYNNRLKEMLSAIDNLAFMNMDERLLNYLSEKSK
                     VLNSNEIHSTHQEIAYDLHTSRVVVSRLLKALENKGKISLNRASIVMLK"
     gene            12842..14482
                     /locus_tag="PLQ70_03070"
     CDS             12842..14482
                     /locus_tag="PLQ70_03070"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF010037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HQE33625.1"
                     /translation="MKILIIGGVAGGATAAARLRRLSEENEIIIFEKGEHVSFANCGL
                     PYHIGGVIEERSKLLLHTPTSLKTRFNLDVRVFNEVLKINKEAKTVEVRNLITKETYT
                     ESYDKLLLSPGAEPFRPNIPGINSNKIMTLRNVADMDRIIAKTKDLKNIAVVGGGFIG
                     LEVAENLVEKGLNVTVIELGNQVMAPVDYEFAKMVQHHAAAKNLNILVNTGVEAFEEK
                     GNEIELKLNNGTTILADAVVFAIGVKPENALAKSAGLDLGVTGGITVDDFMQTSDSNI
                     YAVGDAVEVSHYINQAKVLIPLAWPANRQGRIVADNITRGNKISYKGTLGTSIIKFFD
                     LTVAATGLNEKLLKRYNIPYRTVTVTRGNHASYYPGATNIVLKMNFGEDGTIYGAQAL
                     GQEGVDKRIDVIATAIKGNLTIYDLQEIEIAYAPPYNSAKDPVNILGYVAENMLNDDV
                     RFINYDQLDDYLLNENAVLIDVRSKTEFENGAIPNAINMDVDTLRDHLDFFDKNKKYV
                     IYCQIGLRGYLAQRILRNNGIYSVNLNGGYGLWKPLNS"
     gene            complement(14555..>14690)
                     /gene="rocD"
                     /locus_tag="PLQ70_03075"
     CDS             complement(14555..>14690)
                     /gene="rocD"
                     /locus_tag="PLQ70_03075"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HQE33626.1"
                     /translation="FSEYGLLAKPTHGNKIRLAPPLVITETQIQECLVIIEKALNDFK
                     "
BASE COUNT         4548 a         2796 c         2542 g         4804 t
ORIGIN      
        1 tttgtcttat aacgttatca acgataaatc agataatttc caggttggtg ctgccctaac
       61 ctattcgttg tccgaccata aaaacacttt caatttccaa gttttaaatg ctagaaccaa
      121 gacttttcaa gaacaatacg gaaataccgc gcctcctgga attactcaaa caaaaacacc
      181 tttgagtgtg gttgcgaatt ggaaagggca tctttttgat ggaaaacttg aaactaccta
      241 cagttttgct catttcattg atgccgaaaa ggccaataga aattattttg gactaggaaa
      301 taaatttaaa accggaaaac tgattctttt ttatgacttt caatatagta aagaagattt
      361 agaccaaaaa tgtgtaatta cgaatgctgt aaaaacgaaa tacagctatg cagctcaaga
      421 cgtttcctat attgataact ggatacgagc tgaatataaa atccaaccca aagtcgatat
      481 attactttct ctaatgacta acaattccta ttggaatgga aatccagatc ctaacaaaac
      541 caatcgtttg ctaacttcat acggtattat tccaacaatt gaatacactc cttttttgga
      601 tttgaattta aagttctatg ttggttatat tgcaaaaagg tatgattatt ctagttatgc
      661 agaaagcaat ttaaatgcaa aagacatttc tacaggtcaa ttgagttttg gtattattgc
      721 tccgctccta gttttgtaac gcaacgactt ttattaattt ttttaattca cttttttata
      781 actccaaatc gcaattccgt tcaatgcaaa agcatagccg acaatcataa aaaacggctt
      841 ggaaatatca gaaaacgtgg ctcccttgag catcaccatt cggataattt ccacaaaata
      901 ccgaatcgga ttgagcaaag taatattttg tgcccattga ggcatacttt caattggggt
      961 gaataatcca ctcattaata tgaagataac cgtaaaaaac caagctacaa acatcgcctg
     1021 ttgttgggta tccgaatgat tggaaatgat tagtccaata ccaagaatga ccaacaaata
     1081 aaccgaagta aagaaataaa tcaagccaat atttcccaat atcggaacat tgaaaacgac
     1141 tttggcaata gtcaacccaa cggtcaaaat tagcaatccc aatacccaaa acggaaacaa
     1201 tttcccgatg atgaactggt atttttgaat gggcgtcacg ttgatttgtt ccaaagtgcc
     1261 gatttctttc tcccgaacaa tattcatcga agacagaaaa agtgtgagca tcgtcaccaa
     1321 caagaccaaa attcccggca ccataaaggt tttgtagttc agtgtattgt tgtaccaaaa
     1381 cgaaggaatt gtgacgatat tcaagggttg cacctcattg gcatcgttgt attgagacaa
     1441 ttgggtttga atcttttggt taaaaccgtt gatgatttgc aaaatataaa cactctccac
     1501 tcctgccgcc gctccatcaa tggcgttgat gcttaccgaa agattcgtgg ctttttcggt
     1561 gattaaatcc cgttcaaaat gattgggaat ttcgagaatc atatcgactg ttccggtttg
     1621 catttgggaa tcggcttctt tcttggaaga aaaactttcg atgacattga aatatttcga
     1681 agcttcaaat ttcgaaatca attctctcga agctgtggaa tgatcgttgt caacgtaaga
     1741 gaatttgatg tttttgattt caaaactcgc tgcattggac aaaatcagca attgtaccaa
     1801 gggcaaaacg aagataactg gaagcattcc tttgtttcta aagatttgct tgaactcctt
     1861 ttgaatgatg aatagtattg ttttcataaa tgggacacgg atgacacgga tttaactgat
     1921 gttcacggat tttttaatct tttctctatc atcaattttc tattctctat tttctctact
     1981 ccaacctaat tttatatttc ttgatactta ccgcaagaaa aaacgtcgtc attcccacaa
     2041 gaatcaaggt ttctttccaa atcacgctta tggtggctcc tttgagcaaa atcgccttga
     2101 tgataatgat aaaccatttg gcaggcacaa tattactgat gatttgcaac ggcattggca
     2161 tactcgaaat ggggaaaata aagcccgaaa ggatgatgac gggcaacatc aatcccatca
     2221 aagacagcat catagcggtt tgttgcgagt tggaaaccgt cgagattaaa atccccaacg
     2281 aaagtgccgt gatgataaac aaaacacttt caaaagccaa cagaagcagg ttgccttcga
     2341 taggcatttt gaacacaaaa aagcccaaaa gcagaataat tgtcgcatta ataatcgata
     2401 gaaaaatata cggaaacacc ttgccaacaa tcacttgaaa gggttttata ggcgaaacca
     2461 aaagcacttc catcgttccc aattcctttt cgcgagtgat ggaaatggag gtcatcattg
     2521 ccgaaaccaa catcaaaata atggtcatca cgccaggcac aaagttgaaa acgctcttca
     2581 attcgggatt gtaatagagt tgagtttgtg tttgaatcag gtggctgggt ttgttgtttt
     2641 tattgatttc ttgggtgtaa ttctgcaaaa tcgcattgac ataattcgca atcgtgttgg
     2701 ccatatttgg gtcggtggca tcggtaatga cttgcacttt ggcttgtttg tgggtttgca
     2761 ggttttttat aaaatcggtt tcgaagacca agacggcttt tactttccct ttttgaaaaa
     2821 cgttttcaat ttggttttcg ctactaatgg catcattgat tataaaatac ttcgaagcac
     2881 tgattttgtt gattattttc tgggtttcac tgtcttttga ttggtccaaa atggcaatat
     2941 tcacgttgtt gatttcgttg gtaatggcaa aaccaaacaa cataatttgg gcaatgggca
     3001 tcccaaaaag aatgaacata gtccgtttgt cacgaaaaat gtgatagaat tcttttgtta
     3061 caaaaccgat aaatcttttc attttgtatt gagtaattag taactagtga atagtaatta
     3121 gcttattcca catttcgcgc caattttaaa aaaacatcgt tcattgaatc cacattgtac
     3181 ttcgctttta ggtttttagg cgtgtcaagt gcttcgatac ttccttcgac cataatcgaa
     3241 actcggtcgc aatattcggc ttcatccata taatgcgtag tcacgaaaat cgtggttcct
     3301 ttgttggctt cggcataaat catttcccag aattgtcttc tggtaatcgg atccactcct
     3361 cctgtcggtt catcaagaaa gacaattttc ggttcgtgca acaaggccac ggagaaggac
     3421 aacttctgtt tccaacccaa aggcaaagag ccaaccagtt tgtcaatggt gtcttctaga
     3481 cccaattcgg caaccaatcg agcggttttt tccttgattt gactcctgct caaaccataa
     3541 attccaccaa aaaaagtaat gttttctttc agcgtcaaat cgtcatacat cgaaaacttc
     3601 tggctcatat aaccaatgct tcttttgacc atttcggtat tggttttcac gtcaaaacct
     3661 gccaccaaag ctgctccgga agtaggtttc gaaatcccga taagcatttt catcgccgtg
     3721 gttttcccgg cgccgttcgc accaaggaaa ccaaaaatct ctcctttata cacctcaaaa
     3781 gaaatattgt tgaccgccgt gaagttgcca aattgcttga tcaaattttg aactgttatg
     3841 attttctctt ttgtcatatt ttttaaacca caaattgcac acccgagcga tagcgaacgg
     3901 acgaagtaaa ttagcacaaa tttatttagt cacagattac acgaattgaa cggatttatt
     3961 gttgttgttt taaccacaaa ttgcacaaat tatcacaaat ttattgaaca ctgattacaa
     4021 gatttttgaa attttaaatc aagtaaatct gtgtaaatcc gcttgatccg tttcatccgt
     4081 gtgccatttt ataaatccat aaaaacgtcc tcaatggttg ttattgctgg ttgaattgtt
     4141 atttcctgat gatttttatt cgctaaataa gtttccaaat ctttggtatt aaaattctcg
     4201 tctttatcaa tataatgaat gaattctcca aaggcaaaaa cgctgtacct actaggataa
     4261 tgctttaaat ccagaatcaa ttggtgcatc ttttcggttc gaatggcata aatcgtcttg
     4321 tcatattttt ggatgatatt ctccggagaa tcgattttca ggattttccc tttttggatt
     4381 aaagcgattc ggtcgcacat agccgcttcg ttcatataag gtgtggaaac caaaatcgtg
     4441 atgccttttt gctgcaatct tttgagcatt tcccaaaatt cttttctcga aacgggatcc
     4501 actcccgtgg tgggttcgtc tagaaacaag actttgggtt tgtgaatcaa ggcgcaacac
     4561 aaggccagtt tctgtttcat tccgcccgaa agcgctcctg cccttctggt tttgaacggt
     4621 tcaatctgaa cataaatatc cctgattaaa tcgtaatttt cctcgatgat agttccaaaa
     4681 atcgtggcga aaaaagtcag gttttcttcc acggacaaat cttggtacaa agaaaatttc
     4741 cccggcatat accccaccga atctcgaatg gctttataat cctttaccac atcaaaaccg
     4801 gccaccgtcg ccgaaccttc attggccaat aaaagcgtgg tcaaaatcct gaaaatagtg
     4861 gtcttccctg ctccatcagg accaatcaat ccaaacaatt ccccttcttt tacctcaaaa
     4921 gaaatggctt caaccgcttt ggttttgttg taggatttgg aaatattttg gacggttata
     4981 ctcataattc aatgccaaaa atcaatttca atttcaatga taatttcaaa aatcaatttg
     5041 tcttttagct tttttagatt tctttagatt ttttcattca ttaacccaca tctcggcggg
     5101 cattccaatt ttcaaaatgc cgtcgttttt tacttttact ttcacggcat acaccaagtt
     5161 tacccgttct tctttggttt ggacgatttt tggcgtgaat tctgccgaag atgcaatcca
     5221 agaaatgatt ccgggatagg ttttcattcc tgcatcagca tcaattttta cgtttacttt
     5281 ttgacccact tttatttgcg ccaattgggt ttcgctgaca taaacccgca aggtcatttc
     5341 ggaaatatcg gcaattttat aaataggttt tccaaaagcg gtcacttcgt taggttcggc
     5401 atatttggac aaaaccgttc ctttgatcgg attgatgatt ttgctttttt ggatttggtc
     5461 gttaattttt tcgatctgga catcgatgga tttgacttcg ttcacgatgg gtgcattttg
     5521 ggtttgcaca ctttttattt gttccagaat gacgctcact ttgccgtcaa tttcatccac
     5581 ttgacgcttg gtggcggcat tttcggcaaa catattttgg attctttctt tctctatttg
     5641 tgccgttttc aattgttcct gcaagacttt aatttgcgac aaaacattgg ccgatttcga
     5701 ataaaccgta cttttcgacg ccatcaattg ttgcttgttg aaatacaatt gagtggtatc
     5761 aatcaatccc acttgaattt tgggttccaa aacgtcacct tcttccagtt ttaaatagtc
     5821 tatttttcca ctggattcag ccgaaatagt gacttccgtg gcttcaaaat tgccgtaacc
     5881 atcggctttg tcgttgtttt tgttgcaaga aaacaaactt gccagtatca agaatgggat
     5941 tatatttttc attatcattt tgtttatagt tagcccttcg actgcgctca gggtgacaca
     6001 agaattacgt ttaatttcct tttactactt gataattcgc tttggccaaa gccaattgaa
     6061 tttcgtgtac tttttgattc gttctggctt cgtataaatt cgtgaattcg accaaatagt
     6121 ccgaagtggt aatcacgcca tttttcaatt gggcattggc cgatttttcg acatattccc
     6181 tcaaaccaat aatctcggta tcggttttga tgatttcttc cgttttcttg atttcgttgt
     6241 tcatttcctg taattgcagg gaattgttga gcaaaaaggt ttctttttcg gtttcgacaa
     6301 tttttgatga aacggtcaaa gcgtcttttt cggctttggt tttgttccaa tcaaaaacat
     6361 tccaattggc tctcaatcca accatcaaaa tgggttgaaa cgaattgtca accatattca
     6421 agcccggatt tccgtaacct accagtccaa aagcgttgat tttaggcaaa ttatttttgg
     6481 caataacggt ttttgaggct tcaatttgct ggttttgcaa ttcgaaatat ttcaactctg
     6541 gacgattgtt ttgggtgttt tcttcggcaa gaataatcgg tttttgcaaa attgtcgttt
     6601 ctggaacaac cgtaaaagtc aaggacgaaa gattttcgaa caggcgtttt ttgtcgaata
     6661 gaatttccga aagttgttgc ttgattttca agttttcggc ttccaaaact ttttcagaag
     6721 cgggaagaat cgctccaaat cgaatccccg atttgacttc tttgatcctc gaaatcaatt
     6781 gttcttgttt ggaaatcaac agttctttgc gttcctgcaa aagcaaaata gaaaaataca
     6841 actggttgat tcgggttttg agttggtaca aattaacttc aacttgttgc tgttgggttt
     6901 tggtttgcgc ttccttgagt tggacattag catcaatcaa accaccgttg taaaggagtt
     6961 ggttgacatc caaattggct cggtattggt ctttattcaa aggttctaaa attggattcg
     7021 gaatcgtggt cacctgattt tggtagcttc cttgggcaac caaatcaatt ttggggagtt
     7081 tgcctgtttg caaagcatcg acttccaaat tggatttttg ttgtagcaaa tccatctgtt
     7141 tggcaatggg ataattggtg ttggccaaag cataacaatc ttccaaagtc aatgattgtt
     7201 gggcgttgac acaaaagaca accaacaaaa gaaatgtaat taatattcgt ttcatctttt
     7261 tatggaattt atgatttgtt cggcaatgct ggtttttcgt tcttccatca tttgattgaa
     7321 ttcggaatcg gaaatttgga gcattccctt gaccatcatt tgggcagcaa aaggaaaaac
     7381 cgtcatcgaa atcatattca tcaaaagttg tttgggatta atgggtttga tgattccatc
     7441 ggcaatttcc ttttcaattt gggcaattaa taatgttgga ttgggtctgt ttgcattctt
     7501 taaaaacgac atcacaaact cgggattgtt gttcatttct tgaatcacga actggggcaa
     7561 atagggattt tgaattacaa acgagatata actatgggta aactttctga ttttgtcaaa
     7621 aacgcagtca tttgaactga atatctcgtt gatttgtggt gccaattgac cgaaagcatt
     7681 cttaaatacg gcttgaaaca gcaattcttt gttcttaaaa caataatgta gcatcgcttt
     7741 attgatacct gcttcatctg caatttcctg cattcgagcg cctgcaaaac ccttcttttg
     7801 gaaaacgatt cgggctgcat tgaatatttt ttcttcggtg gtcatcgtgt tttaactata
     7861 tagtttaacc atttggttaa caaagataaa taaaaaaagt attccgtgat gaaatacttt
     7921 aaaatattta tttcttttga aattcaatga acaactctga attagctcta ggtataattg
     7981 aatttagaca caaatccatt ctcaaaattt cgaacttagt cacaaaaagt tgctcatatt
     8041 cggtttggct tccgccaaaa ggtggtccag attcaaatgt tttgttgaac aatagtccca
     8101 caagttttcc tttggaagat aaaagctggt gaattttcga aacataattt tcacgcattt
     8161 tgggcggtaa tgcgcaaaaa aaagtttgtt ctataatcaa atcatattcg ccttgatgtt
     8221 cgaaaaagtc gcccaatata attttaatac taggattgtt ttcaaattta ccttgaaggc
     8281 tttcaattaa agttggagca atatcaataa cagttatatc agtaaacccc tggcttagca
     8341 gatattctgc ctcataggaa ttgccacaac caggaattaa aattctagaa tctttatttt
     8401 ctataacttc gaaatgttct tttaggggcg gtgaaacttt gcctaaatcc caaccagtag
     8461 ctttggtttt atattgattt tcccaatatt cttggtctaa acttttttca ctgtttctca
     8521 taatccattt tataattact aatatttttt tcgctatcgt ggatcatttt tttatgtttg
     8581 aaataattga aaacgccaat acaaaaagta gaaacgaact gatgaaaaag aacatttcaa
     8641 ttataaaatg aaatcgttct cttaatatca ggcctttatt tatcttgttt tcaattattt
     8701 attatttctt tttgcaagta gaaattccca acggaacata caaaggacaa aaactaatca
     8761 aactggtcag tagaaaaata attgcaaatg cacctaaaac caacgcagct gtaccttcta
     8821 tagcttttgt aaaatacaaa acaccaataa cgattgcaaa aagaatacga gaaattctgt
     8881 ctgccgaacc catgtttttt ttcatactat ttgaatttaa ttgattacaa attaagattt
     8941 tttggatgat ttaacgaaca tattaaaaag caatccgccc acaacagcac catacaaact
     9001 actatttaat ggttttgaag ttatagcgca agttcctgat gaacagccta cataaaaata
     9061 atagagataa ccagcaatag ccccaacaac gactccaatt cccgtaataa ttagtgcttt
     9121 tttagtcatt ttaattgctt ttttctacga tggtttttac aatttcttcc tttgacataa
     9181 cacccgattg acgccacaat tgctttccgt tttgaaacaa aatcattgtt ggaacgccgc
     9241 gaacttgata tttcgaagcc aattgttggt ttttatcgac atctattttt ataatagtga
     9301 ttctttctcc taagttatct tttacttgtt tcaaaatggg tgcaagtgtt ttgcaaggcc
     9361 cacaccaagt agcaaaaaaa tcaatcaaaa caggtttgtc agattgaata attgtatcga
     9421 aactagtgct catttttttg aatatttatt ctttggaacc gtacagccat taggaccaca
     9481 gcctaaatta aaaatcgctt gaaacaaaaa gaacaaacca aaagcgataa aaaaccactc
     9541 tcgggttgtg taagcttgaa tgaacaaaaa cagcgcaaaa gccaatctag cccatcgcat
     9601 tagatgccaa tttgaaaaca ataattgttt cattttttta aagagaatga tgaattccgt
     9661 atattgtttt gatggctgtt tttttgatgg cactgtatcc accagccaca ttaattaaat
     9721 tgtgataacc acgagctttt agtatagaag ccgctatcat agaacgataa ccgccagcac
     9781 aatgcaaata aaattcttct tcctttggaa attcggctaa atgatcgttg atattgtcta
     9841 atggcgttga agaaacatct tcaatatgtt ctttttcata ctcataatct tttctaacat
     9901 caaaaatgat tactttttcg ttttcttcct tggcttcttc tacaatttct tctaacatca
     9961 cagccgaaac cgaattgact gtatcgtatt ctaaaccttc atttttccaa gtatcgaaac
    10021 ctccttttag atagcctaat gtattgtcat agcctactct tgctagtctc aagatacttt
    10081 cttcttccct gcccaaaggt gttatcagta atattggttg tttgatatcg ggaatcaaag
    10141 ctccaaccca aggggcaaaa tctccatcaa ttccaataaa aatagatcta ggaacgtgac
    10201 ctttggcata ttcatcttga tggcgaacat ccaagattac cgtgtcaatt ttattggcca
    10261 ccatttcaaa agtgttcaca tcataggcaa tcgcatctcg aataatatct tctacatttt
    10321 catagccttc tcggttaagc tgagcattta aagggaaata aagcggtgga ggcaataaac
    10381 catcggtcac ttcttgaata aattcttctt tggtcatatc ggctcgaagc gcataattga
    10441 ctcgtttttg ctcgccaata gttcccacag tttcggtgct caaatttttt ccacatgcac
    10501 ttcctgctcc gtgtgctgga taaacaatca catcgtcagc caagttcatt atcttggttc
    10561 gtaaactatc atataaaatt cctgctaatt gttctttgtt caaattagca aatttctggg
    10621 ctaaatcggg acgtcctaca tcacctaaaa ataaggtgtc accgctaaaa agagcataat
    10681 cttttccgtc tttatctttc aaaagatagc aaacactttc tagagtatgt cctggagtgt
    10741 gtaaaacggt taaagtgatt tctcccaatt taaatacatc gccatcagca gcaatatggg
    10801 acttaaattt ggtttctgct gtagggccgt aaatgattgg agctccagtt ttctctgcca
    10861 aagtaacatg accacttaca aaatcggcat gaaaatgcgt ttcaaaaata tatttgattt
    10921 ttgcgttatt tttattcgct ttatcaatat aaggctgcac ttctcgcaat ggatctataa
    10981 tggcgacttc tcctttactt tcgatataat aagcgccttg ggccaaacaa cccgtataaa
    11041 tttgttttat tttcatcttc caaagtgtat ttatactgta aaattatctt tttttttgat
    11101 tgaagctaat ttcaacttca acattttagg aaattattat gaccagaaaa gttctctggt
    11161 tatgataaag attcccattg ccaaaacaaa ccaaccaaaa cctattttaa gtttttcttc
    11221 ttttatcttt ttggaaataa aattccccat aaaaatacca acaattgatg ccgcagtaaa
    11281 gaataacaac aactcccaat caatgatttg atttcccata acatcgcctg taaagccaat
    11341 taatgactgg gatgaaacta taaaaagtga agttccaaca gccattttta ttggtgtttt
    11401 tgcgaacaac aacaaggttg gaacaatcaa aaaaccaccg ccagccccaa caaatccaga
    11461 aagcaatccg ataaaaattc ccataaatgt tagtttataa taattgagtt tttcattctc
    11521 aaaaatcact ttttctttaa aaggttttat catactacct gaagctagaa tcatcacaat
    11581 agcaaaaaca accataatca gaatcgattt gctcaaagta aaatcatcgg tcgaaaacaa
    11641 aatatcagga atgtttggaa taattatttt tcgactaaca aaaaccgaaa ttacggcagg
    11701 aacaccaaac aaaaacactt tattgaaatc tactaatttt tctttggctt tatgaattcc
    11761 accaactaat gaggttgtgc ctataataaa aagggaatag gctgttgcca atgtaggttc
    11821 gattcccatt acatagacca aaattggaac cgaaagtata gaaccaccac tacctaacat
    11881 tccgagtatc atcccaacaa atactgctaa aacatatcca ataagttgag atatttccat
    11941 ttataaaaat ttataaaccc aaaattaaag tattaatttt tccctttttg caacaaatgt
    12001 tacttatttc agcattacga tggaagctct attcaaacta attttaccct tattttctaa
    12061 tgcttttagt aaacgagaaa ccacaactct tgacgtatgc aagtcataag caatctcttg
    12121 atgagtagaa tgaatttcat tagaattaag cacttttgat ttttctgaaa ggtaattcaa
    12181 taaccgttcg tccatattca taaacgccag attatcaata gctgacagca tttcttttaa
    12241 acgattgtta taactattaa aaacataatt tctccaactg ctatacttgc ctagccactc
    12301 ttccattttg ttgacaggaa tcataattaa ctgtcctttg gtttcggcaa cggctctaat
    12361 ttcgcttttg gtttcaccca aacagcaagc cattgtcatt gcacaagtat cacctttttc
    12421 aataaaatac aacaacaatt ctccttcatc aaaatcctca cgcaatattt tgatagctcc
    12481 tgaaataagc aaaggcatct ttctaatcga atcgccaaaa tctattaaaa tatctccttc
    12541 tttgaaatct ctcaataaag aagtagccac aatttcctca attaatttat cttcaaataa
    12601 gtacccataa gtttgttgga gtaactcttt cattattgtt tttgattttt attggacgta
    12661 aaattcgtta aaaatttcca ctttaaccaa taaaacacca aacactttgt ctcggctcat
    12721 actatttata tcttttggtt gatggaatcg ttttatttaa aatcatttcg ctttatgtaa
    12781 caaaagctac aaatcaaaag aatttgattg atgaaatttg cattataatt taaacacaaa
    12841 catgaaaata ctaattatag gtggagttgc cggaggagca acagccgcag caagattaag
    12901 aagactgagt gaagaaaatg aaatcatcat ttttgaaaaa ggagaacacg tgagttttgc
    12961 caactgtgga cttccctatc atattggtgg cgttatcgaa gaacgctcta aattattgtt
    13021 gcacacgcct acttccctga aaacaagatt taacttggat gttcgtgttt ttaatgaggt
    13081 tttaaaaatc aataaagaag caaaaacggt agaagttaga aatttaatta ccaaagaaac
    13141 atacaccgaa tcctacgata aattgttgtt gtctccaggg gcagaacctt tcagacccaa
    13201 cattcccgga attaattcga ataaaataat gacattgcga aacgtggccg atatggatcg
    13261 aattattgcc aaaaccaagg atttgaaaaa tattgctgtg gttggtggtg gtttcattgg
    13321 cttggaagtg gcagaaaatt tggtcgaaaa aggcttgaat gtcaccgtta tcgaattggg
    13381 aaatcaggtg atggctcccg tggattatga atttgccaaa atggtgcaac atcacgctgc
    13441 tgccaaaaac ctgaatattc tcgtaaatac tggtgttgaa gcttttgagg aaaagggaaa
    13501 tgaaatcgaa ttgaaattaa ataatggcac aactattttg gctgatgctg ttgtgtttgc
    13561 cattggcgtg aagcccgaaa atgctttggc gaaatccgca ggattggatt tgggtgttac
    13621 tggtggaatt actgtagatg attttatgca aacttccgat tcgaatattt atgcagtagg
    13681 cgatgctgtc gaggtttcgc attacatcaa tcaagccaaa gtattgattc cactagcttg
    13741 gcctgccaat cgtcaagggc ggattgtggc ggacaatatt acgagaggaa acaaaatttc
    13801 gtataaaggc actttaggaa cgtctattat caaatttttt gatttgacgg ttgccgctac
    13861 gggactcaac gaaaaactat tgaaacgcta caatattccg taccgaaccg tgaccgttac
    13921 ccgtggcaat cacgccagtt attatcccgg agccacgaac attgtcttga aaatgaattt
    13981 tggcgaagac ggaaccattt atggtgcaca agctttagga caagaaggcg ttgacaaacg
    14041 catcgatgtg attgcgactg ccatcaaagg gaatttgacg atttatgatt tgcaagaaat
    14101 agaaatagcc tatgctccac cctacaattc cgccaaagat cctgtgaata ttctaggtta
    14161 tgtagccgaa aatatgctga acgatgatgt aagattcatc aattacgacc aattagatga
    14221 ttatctgttg aatgaaaatg cggttttaat tgacgttaga agcaaaaccg aattcgaaaa
    14281 tggagcgatt ccaaacgcca tcaatatgga tgtggatact ttgagagatc atctggattt
    14341 tttcgacaag aacaagaaat atgtgattta ttgccaaatt ggtttaagag gttatttggc
    14401 gcaacgcatc ttgaggaata acggtattta ttccgtgaat ttgaatggcg gttatggact
    14461 ttggaaaccg ttgaatagtt agggaaaata ttatgactag tcataaataa tctgtaacga
    14521 ttattcagga ctagtcaaat ttgaatattt aaaattattt aaaatcattc aacgctttct
    14581 caataataac caaacattcc tgaatttgag tttcggtaat gaccaatggc ggtgctaatc
    14641 gaattttatt gccgtgagtg ggtttggcta ataatccgta ttcgctgaat
//
LOCUS       DAOSNN010000033        14545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_4171_length_14545_cov_5.111456, whole genome shotgun sequence.
ACCESSION   DAOSNN010000033 DAOSNN010000000
VERSION     DAOSNN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14545
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4171_length_14545_cov_5.111456"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLQ70_03080"
     CDS             <1..192
                     /locus_tag="PLQ70_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQE33627.1"
                     /translation="MKRRGGMGLSLAIGIALAFSFVFFDKIFGTLAEKSSFPPLLAVW
                     IPNLVFGILAVFLLRNAKR"
     gene            179..1063
                     /locus_tag="PLQ70_03085"
     CDS             179..1063
                     /locus_tag="PLQ70_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQE33628.1"
                     /translation="MQKGNLKSYLNLHLIVFIWGFTAILGALISIQADALVWYRMLFA
                     SVFLFLFILFKKESFQIPIKEFFKLIFVGLLIALHWIFFFEAIHVSNVSVTLAVFSLG
                     AFLASILEPIFYGRKVLWYEVFFGLIIIAALGMILQVEINYFEGIMYALVSIIIGVLF
                     TLMNGKLIEKHESSVITFYEFLAGVFFITIYFFFTSKFNVDFFILTTNDWIYLLILAS
                     VCTAYAFTASVKVMRVLSPYTVMLTTSLEPVYGIILAYFILGGKEKMSVEFYIGALLI
                     VLTVILNGIVKHYLSNKE"
     gene            1179..2132
                     /locus_tag="PLQ70_03090"
     CDS             1179..2132
                     /locus_tag="PLQ70_03090"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQE33629.1"
                     /translation="MEYLDFELPIKELEDQLDKCLVIGQESEVDVTNTCKQITKKLEE
                     TKRSIYKNLTAWQRVQLSRHPNRPYTLDHIKALCGETFLELHGDRGFKDDKAMIGGLG
                     KIGGQSFMLIGQQKGFNTKTRQYRNFGMPNPEGYRKALRLMKMAEKFGIPVVTLIDTP
                     GAFPGIEAEERGQGEAIARNIFEMVRLKVPIICVIVGEGASGGALGIGVGDRVYMMEN
                     TWYSVISPESCSSILWKSWDYKEQAAEALKLTSADMKKQKLVDDIIPEPLGGAHFDRE
                     SAFKTVEQYIIKGFNELKDLSTDELVAQRMDKYCKMGDFKE"
     gene            2273..3814
                     /gene="dnaB"
                     /locus_tag="PLQ70_03095"
     CDS             2273..3814
                     /gene="dnaB"
                     /locus_tag="PLQ70_03095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQE33630.1"
                     /translation="MENVKNINPIRIDKTTIINLEKGKLPPQVVDLEEAVLGAMMIDK
                     KGVDEVIDILQPDAFYKEGHKHIFEAIVQLFTDTQPIDLLTVSAQLRKNAKLDLAGGD
                     LYLIQLTQKISSSAHIEFHSRIILQKFIQRSLIRISTDIIEDSYDETTDVFDLLDKAE
                     SKLYEVTQGNIKRSSETAQSLVLQAKKRIEEISKQEGLSGVETGFTNLDKLTSGWQPS
                     DLIIIAARPAMGKTAFVLSMARNIAIDYGHPVALFSLEMASVQLITRLISSETGLSSE
                     KLRTGKLEPHEWTMLSTKVKNLEKAPLFIDDTPSLSIFDLRAKSRRLVSQHGIKIIIV
                     DYLQLMTAGGNGKGGGNREQEISTISRNLKALAKELNVPVIALSQLSRAVETRGSSKR
                     PLLSDLRESGAIEQDADIVSFLYRPEYYKIDEWDDDEASPTAGQAEIMIAKHRNGGIE
                     NVRLKFIGHLGKFDNLDDYNSSYDDLPSSMNQDENPFQTKNLPSPNEAFGSNLNEEDD
                     DLVPF"
     gene            4107..5369
                     /locus_tag="PLQ70_03100"
     CDS             4107..5369
                     /locus_tag="PLQ70_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HQE33631.1"
                     /translation="MSSKKIFFIVVLLTSFAAKASFILLPMDETTQKNHLKAYGITYW
                     CIEKKYKASWLLNYRGGAFLLADASEIRKECQIRGVSFEVLSDDETNQILAEIASPSQ
                     NMEAVVLEKAPKIAVYTPKGKQPWDDAVTLVLTYAEIPFTPIYDEEVLSDQLILYDWL
                     HLHHEDFTGQYGKFFGSNRNSPWYIDQKKEAEILATKLGYKKVSEAKGAVAKKIRDFV
                     IGGGFMFAMCSATDSFDIALSADGVDICEPMFDGDPSEFNYQSKIQYGNTFAFKDFTL
                     DRKPEHYEFSDIDMTEKRRIPMEKDYFTLMEFSAKWDPIPSMLCQNHTQLVKGFMGQT
                     TAFDQNLIKSNVLIMGSCELNGEARYIHGEKGKGMFTFFSGHDPEDYQHRVGDPVTVL
                     DLHPNSPGYRLILNNVLFPAARKKKLKT"
     gene            complement(5819..6025)
                     /locus_tag="PLQ70_03105"
     CDS             complement(5819..6025)
                     /locus_tag="PLQ70_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33632.1"
                     /translation="MKNLNLIILLILGYQLFSCNPKTEKKQPTIYNNYNQNYKFDDDE
                     MQKRNTKKVKDTKQTPKYQTKKKA"
     gene            complement(6040..6363)
                     /locus_tag="PLQ70_03110"
     CDS             complement(6040..6363)
                     /locus_tag="PLQ70_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33633.1"
                     /translation="MEKSPKINLDEQKKAVQTNCPKKISTKKWDKRLADFKNYTKEYL
                     KQYKKSLKGNFISFCKYPYLKAKSEALYEELNEAQSQGILTEKQLIQKSKTEIKIGSS
                     PSTQV"
     gene            complement(6442..6651)
                     /locus_tag="PLQ70_03115"
     CDS             complement(6442..6651)
                     /locus_tag="PLQ70_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HQE33634.1"
                     /translation="MNRYTVNDRNIISVGYDEVHKTLEIEFKLQVIHQYFGVTLDEFV
                     ALMKAPEIETHYFNNVYCHYYYDVD"
     gene            complement(6851..7519)
                     /locus_tag="PLQ70_03120"
     CDS             complement(6851..7519)
                     /locus_tag="PLQ70_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQE33635.1"
                     /translation="MFQKVLVAEDLDSISIAVVQAIEDLGITEIHHAKYCDDALLKIK
                     KGLHDNSPYDLLISDLSFKLDHRDNQLGSGDELIEAVKKIQPHIKTIVFSIEDKSFRI
                     KSLFENLEINAYVSKGRNSIPELQKAIQRVYNTDEKIVSTELSYALRDKSLFEIENYD
                     ILLLKSLSKGLTVDEIGLDFKNSGIIPNGGSSLEKRISKLKIFFKANNNVHLIAIAKD
                     LGLV"
     gene            complement(7531..9255)
                     /locus_tag="PLQ70_03125"
     CDS             complement(7531..9255)
                     /locus_tag="PLQ70_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQE33636.1"
                     /translation="MIHKKLHSPILVLATLFLLLQSCDKKPIINPKKEKNLAEIDRLI
                     DLGDQYLEKVNYDSAYYYHNKAKSLCDVKTDNTRIIYSLARMSEIQQNQGDYSGSETT
                     AIEALSFVNETMDPSYESSIYNLLGVVYLKLFDYDNSLYYYKKGLNLKIDKTRKLKIQ
                     HNIAVVYMAKHDYNQAIQILLPLTLKKEVINDPAPYSKALDNLGFSYFKVGNPKGIDY
                     LNQALKIKEKLKDDWGMAASYLHLTQYYKPTNPKLANVYARLAFEKATKVNNIDDRLE
                     ALALLIQSSSGNQSKFYSEKYIHLNDSINRVRQNARNQFAKIKYDSKKEKEENLLLKN
                     QKSENLLELEIQKNKNLLLRFLIAIGILSTIFLYYFLKAINKKEKIQTSYETETRIAK
                     KLHDELANDVFQTIAFAETQDLASAQNKETLLNNLDTIYTRTRNISKENSAIETGENF
                     VPNLKEMMARFNTSEINILINGLDDFDWTLLENTKKITIYRVLQELLVNMKKHSKCSL
                     VAISLKKEENFLHINYSDNGLGASLNEINFKNGLQNVENRILAQNGTITFDTKSNKGF
                     KVSFSIPI"
     gene            complement(9433..11565)
                     /locus_tag="PLQ70_03130"
     CDS             complement(9433..11565)
                     /locus_tag="PLQ70_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQE33637.1"
                     /translation="MKNFSILALSCFITIATFSQTKKLTLEQGVLEQNRLFRADKLIG
                     FQWIPNTTNYTYYTDNYTKMVAASATDSNTIETVTLADINSALGTKLKNFFGIQWIDS
                     NALLVSESGKQYAYLTTSKKGTLMQEPKSSCQNLTYDIKKRNLAFTENNNLYYYNVNK
                     EIVQVTNETNEAIVSGQFFARSEFGIRDGIFWSPKSNFLAFYQKDQTEVADYPILDIN
                     ETPGKLVNIKYPMIGQKSEKPRVGIYNLASKKTVFISPKGNLDDYLTNLSWSPDEKYV
                     LIAELNRAQNDMSLNVYDAQTGNFVRTILNEKNDNWVEPEHPAFFPSNANNNFVWISE
                     KSGFNNLYYYSIEGRLIKQLTQNKFPVREIIGSNTAGNEIYFKATGENPTNMLAYKVD
                     LNGKQTLITKDQGVHNCIPNFDGSYFFDEYSNHSTPSKSLLYSKNGSAKTLLESSNKY
                     ADFQMGTAEIKTIKSADGTTDLYTRLIKPSNFDPTKKYPVLVYVYGGPHAQLITNSYL
                     DGANLWMYWMAEQGYLIFTVDNRGSDNRGFAFESVIHGRLGVNEIEDQIKGVDYLKSL
                     PYVDENRLAIHGWSFGGFMTTSIMLRKPDVFKVGIAGGPVTDWNYYEVMYGERYMDTP
                     AENQKGFDEASTLNYVKNLKGKLLLIHGTSDDTVVMQNNFVLLKKFIEAEKQVDFFAY
                     PMHEHNVIGKDRVHLMTKVLNYVIENNK"
     gene            complement(11674..12474)
                     /locus_tag="PLQ70_03135"
     CDS             complement(11674..12474)
                     /locus_tag="PLQ70_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQE33638.1"
                     /translation="MKQIILFLFLFTTTLFSQPKLLSWNLENFGSSKSNTEITFIANT
                     LREYDIVAIQEVVAGDGGAQTVAKLADELNRKGAKWDYTISEPTSSSAYKTERYAFLW
                     KTSKIKKIGRAWLEKKYHLEIDREPYFCTFQYENKQFTIVNFHAITKKMQPETEIKYF
                     KFLPEEYPHLNLIFVGDFNCPQSHTVFNPLKKMGYQSILVNQKTSLKQKCEYDTCLAS
                     EFDNMFYNSSKIARLNSGVLPFYKNFNSLKEARVISDHIPIWFEFFLN"
     gene            13028..13522
                     /locus_tag="PLQ70_03140"
     CDS             13028..13522
                     /locus_tag="PLQ70_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33639.1"
                     /translation="MTASGKNGLLLNVVLQPNESGFIVTSNEALFTLPTKLNGIDEQI
                     TAIEGEWKVFFDPKWGGPGEVFFTNLTDWTKNVDDRINYYSGTSVYRKSIKLDQPISG
                     TQVLLRFPQLGSIARVLINGKEVSTVWCSPWEADITLYIQKGQRIPSIHDLTGNLLLE
                     TKKN"
     gene            13519..14019
                     /locus_tag="PLQ70_03145"
     CDS             13519..14019
                     /locus_tag="PLQ70_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33640.1"
                     /translation="MIEDVAIKEFVKKEERVKKEDKKEEIVFQELNKKSVSKKIAFAV
                     IPLFVFGAAGFGIKDIFFRDKNCMVWVKNHYEAAECDAIKDTAEVCPFNQGILDNFKK
                     ISVCDTTTFFKNGDTDNPLVWYGKSPDKKEYEYFNQPGLHPETGKTLKPISKYIIGKY
                     ILKKNE"
     gene            complement(14231..>14545)
                     /gene="rplT"
                     /locus_tag="PLQ70_03150"
     CDS             complement(14231..>14545)
                     /gene="rplT"
                     /locus_tag="PLQ70_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQE33641.1"
                     /translation="RARRKKIMKQAKGFFGRRKNVWTVAKNAVEKAMCYAYRDRKANK
                     RNFRALWIQRINAGARLEGMSYSQFIGKCKANNIELNRKVLADLAMNHPVAFKAILNK
                     VK"
BASE COUNT         4519 a         2323 c         2625 g         5078 t
ORIGIN      
        1 atgaagcgaa gaggagggat gggattgagt ctagccatcg gaattgcatt ggcattttct
       61 tttgtgtttt tcgataaaat attcggaacc ttggccgaaa aatctagttt cccaccatta
      121 ttggccgttt ggattcctaa tctagttttt ggaattttgg ctgttttctt attacgcaat
      181 gcaaaaaggt aatttaaaga gttatttaaa tcttcattta attgtattta tatggggttt
      241 taccgccata ttaggagctt taatttcaat tcaggccgat gctttggttt ggtatagaat
      301 gctatttgca tcggtatttt tgtttctatt tattctcttt aaaaaagaat cttttcaaat
      361 tcctataaaa gaatttttca aattaatttt tgtcggatta ctgattgctc ttcattggat
      421 tttctttttt gaggctattc atgtttctaa tgtctctgtg acattggccg ttttttcttt
      481 aggtgctttt ttagcttcta ttttagaacc tattttttac ggtcgaaaag tgctttggta
      541 tgaagtgttt tttggactta taattattgc agcattagga atgattttgc aagttgaaat
      601 caattatttc gaagggatta tgtatgcttt ggtttcaata attatcggag tattattcac
      661 cttaatgaac ggaaagctaa tcgaaaaaca cgaatcttcc gttattacct tttatgaatt
      721 tctcgccggc gtgtttttta taacgattta cttttttttt actagtaaat tcaatgtcga
      781 tttttttatt ctgactacca atgattggat ttatctcctc attttagctt cggtttgtac
      841 tgcttatgct tttaccgctt ctgtaaaggt gatgcgagta ctttcgcctt atacagtgat
      901 gctaaccacc agtttagaac ccgtttatgg aattattttg gcgtatttta tccttggtgg
      961 aaaagaaaaa atgagcgtag agttttatat cggagcctta ttgattgttt taacggtaat
     1021 tctgaacggt attgtcaaac attacctgtc taataaggaa taattttttt caaggatttt
     1081 aactgtcaat ttatttgtaa ttagatgcta attatacgtt tttgataatt ttaaatttta
     1141 tatttgcatt tcaaatcaaa caaaaactac tataatttat ggaatattta gattttgaac
     1201 ttccaataaa agagcttgaa gatcagttag ataaatgtct agtaatcggt caggaatcag
     1261 aggttgatgt gacaaatacc tgcaagcaaa ttaccaaaaa actagaagaa actaaaagaa
     1321 gcatatataa aaatcttacc gcttggcaac gagtacaatt gtcaagacac cccaatagac
     1381 cttatacttt agatcatata aaagcacttt gtggagaaac tttccttgaa cttcacggcg
     1441 acagagggtt caaagatgat aaagcaatga ttggtggttt aggtaaaatt ggaggacaat
     1501 cgtttatgct aattggtcaa caaaaaggat tcaatacaaa gacacgccaa tatagaaatt
     1561 ttggaatgcc aaatcccgaa ggctatcgaa aagctttgcg actaatgaaa atggcagaga
     1621 aatttggaat tccagtggta actttgattg atactccagg agcttttcca ggaattgaag
     1681 ccgaagaacg cggacaagga gaagctattg ccagaaatat ttttgaaatg gttcgattga
     1741 aagtgccaat catctgtgtt attgttggag aaggagcatc aggtggagca ttaggaattg
     1801 gcgttggaga tagagtttat atgatggaaa acacttggta ttcggtgatt tctcctgaat
     1861 cttgttcgtc tattttatgg aaaagttggg attataaaga acaagctgcc gaagccttga
     1921 aattgacttc tgccgatatg aaaaaacaaa aattagtcga tgatattatt cctgaacctc
     1981 ttggaggagc tcactttgac agagaatcag ccttcaaaac agtagagcaa tatattatca
     2041 aaggcttcaa cgaattgaaa gacttatcaa cagacgaatt agttgcccaa aggatggata
     2101 aatactgtaa aatgggggat ttcaaagagt aaaaacttac aaaattgttc taaatccgaa
     2161 gccttatcgt ttcggatttt tttatgctta ttaacaaaaa aaaattgctt attaacaata
     2221 gtttctaata actcaattgg cttctttttt taattttgct tactttcgct ctatggaaaa
     2281 tgtgaagaat ataaacccaa ttcgaatcga taaaacaaca ataatcaatc tcgaaaaagg
     2341 aaaattaccg ccccaagttg tcgatctaga agaggctgtg cttggtgcaa tgatgattga
     2401 caaaaaaggg gttgatgagg taatcgatat tttgcaaccc gatgcatttt acaaagaggg
     2461 gcataaacat atttttgagg ctatcgttca gttgtttaca gatacacagc ctattgactt
     2521 attaacagtc tcggctcagt tgcgaaaaaa tgcaaaacta gatttggcag gaggcgattt
     2581 gtacttgatt caacttactc aaaaaatatc ttcttcagca cacatcgaat ttcactcgag
     2641 aattattctt caaaaattta ttcaacgcag tttgattcga atttctacag atattatcga
     2701 agattcatat gacgaaacta ccgatgtttt cgatttatta gataaagccg aatcgaaatt
     2761 gtatgaagtg acccaaggaa acatcaagcg aagttccgag acagcacaaa gtttggtctt
     2821 gcaagccaaa aaacgaatcg aagaaattag caaacaagaa ggattaagtg gtgtcgaaac
     2881 gggttttacc aatctagaca aactgacttc gggttggcaa cccagcgatt taattatcat
     2941 tgcggctaga ccagcgatgg gaaaaaccgc attcgtactt tcaatggcga gaaatattgc
     3001 aatcgattac gggcatccag tggcgctgtt ctctttggag atggcctccg tgcagttaat
     3061 tacccgtttg atttcgtctg aaacaggttt gtcttccgag aaattgcgta ccggaaaatt
     3121 agagccacac gaatggacaa tgttaagtac caaagtgaaa aatctagaaa aagcaccttt
     3181 gtttatagac gacacgcctt cactttctat ttttgattta agagccaaat ccagacgttt
     3241 ggtttcgcaa cacggcatca aaattatcat tgtcgattat ttgcaattga tgacagctgg
     3301 aggtaatgga aaaggcggtg gcaatagaga gcaagaaata tcaaccattt ctcgaaattt
     3361 gaaagctttg gccaaggaat tgaatgttcc tgtaattgca ctttcgcagt tatcgcgtgc
     3421 ggttgaaact cgtggttcta gcaaaagacc tttgctttct gaccttcgtg aatcgggagc
     3481 gattgagcaa gatgccgata ttgtttcgtt tttataccgt cctgaatatt acaaaattga
     3541 cgaatgggat gatgatgaag cttcgccaac cgcaggacaa gctgagatta tgatcgccaa
     3601 acaccgtaat ggaggtatcg aaaacgttcg attaaaattt attggtcacc ttggtaaatt
     3661 tgacaatctt gatgattaca acagcagtta tgatgatttg ccttcgagta tgaatcagga
     3721 cgagaatcct tttcaaacta aaaatctccc gtctccaaat gaggcttttg gaagtaattt
     3781 gaatgaggaa gatgatgatt tagttccatt ttaaaataat tgattaattg acaattggta
     3841 attaacaatt cataaatttc aatataacta tccgctttat agtccaagct ccattttcag
     3901 ccacgaattc acgaattgct cttttatata ttattcttag attacactaa tattgtcaaa
     3961 ttcaattaga atttgtgaaa tttcagaaca tttaattcat ctataattag tgaattcgtg
     4021 gctaaaaaga gattaataca aatagcggac agtcataaat ttcattatca attgaaatca
     4081 gtacatttga ttcataaatt tttaaaatga gttcgaaaaa gatatttttt attgttgtac
     4141 tgctgacttc ctttgcggca aaagcttcgt ttattttatt gccaatggac gaaactaccc
     4201 aaaaaaatca tcttaaagcc tacggaatca cttattggtg tatcgaaaaa aaatacaaag
     4261 ccagttggtt actcaattat cgtggggggg cttttttatt agccgatgcg tccgaaatca
     4321 gaaaagaatg ccaaattcga ggcgtgagtt ttgaagtctt aagtgatgat gaaaccaatc
     4381 aaattttagc cgaaatagcc agtccttctc aaaatatgga agccgttgtg cttgaaaaag
     4441 cgcctaaaat tgcggtttat actccaaaag gaaaacagcc ttgggatgat gctgttacct
     4501 tagttttgac ctatgccgaa attccattta cacctattta tgacgaagaa gtcttgagcg
     4561 atcaactgat tctttatgat tggttgcact tgcaccacga agattttacg ggtcaatatg
     4621 gaaaattttt tggatctaat agaaattcgc cttggtatat cgatcaaaag aaagaagctg
     4681 aaattttggc aacaaaatta ggctacaaaa aagtgtctga agccaaagga gctgtggcca
     4741 aaaaaatccg tgattttgtt attggaggag gatttatgtt tgccatgtgt tcggcaaccg
     4801 atagtttcga tattgcttta tcggccgatg gtgttgatat ttgcgaacct atgtttgatg
     4861 gtgacccgag tgaattcaat tatcaatcaa aaatacagta cggaaatact tttgctttca
     4921 aagattttac attagaccga aagccagaac attatgaatt ttcggatatt gatatgaccg
     4981 aaaaaagaag gattcccatg gagaaagact attttacctt aatggaattc tcggccaaat
     5041 gggatcccat tcccagtatg ttgtgtcaaa atcatacgca attggtaaaa ggatttatgg
     5101 gacaaacaac tgcttttgac caaaatttaa tcaaatcaaa tgttttgatt atgggaagtt
     5161 gtgaactcaa tggcgaagct cgttacattc acggcgaaaa aggcaaagga atgtttactt
     5221 tttttagcgg tcacgatccc gaagattacc agcatagagt gggcgaccca gttacggtgc
     5281 ttgacttgca tcccaattcg ccaggttatc gtttgatttt gaataatgtt ttgtttcctg
     5341 ctgcaaggaa aaagaaactg aaaacctaat tctggaagta ttaattcaaa gaaaaagtag
     5401 cccaaatagg aatgtggtcc gaaatgctac gcgcttcttt caaggaatta aaatccttat
     5461 aaaattcaat tacacccgct tgcaatgact tgcattttga agaattaaag tacatattat
     5521 caactaacta ttaaaaaacg acttttacaa tttgtaaaat aatcatattg gttgtctatt
     5581 ttgactttaa ttaagaagta taaaattgaa atagtttctc tccctcctca cttctgcact
     5641 ctcttataaa ctaaataaat caaccacaca gttgctagta caaccattgt taattggaat
     5701 aataatttga agtctatgct gtttaaattt agaatattca tgtttttatt gtttgttttg
     5761 gtaaacctac tgcgattaaa aatcacttcc ttagggtttt cctaatttgg agcatttttc
     5821 acgctttctt tttagtttgg tattttggtg tttgttttgt atccttgact ttctttgtgt
     5881 ttcttttttg catttcgtca tcatcaaatt tgtaattttg attgtagttg ttgtagatag
     5941 ttggttgttt tttttctgtt tttgggttac aggaaaacaa ttggtagcca agaattagta
     6001 aaataattag gttaagattt ttcatgtttt gagcataatt tatacttgtg tactggggct
     6061 agatcctatt tttatttcag tttttgattt ttgtatgagt tgtttttctg tcaaaatgcc
     6121 ttggctttgg gcttcattta gttcttcata cagggcttct gatttggctt tcaaataggg
     6181 atatttgcaa aatgaaatga aatttccttt tagcgatttt ttgtattgtt tgagatattc
     6241 tttggtataa tttttaaaat cggctaagcg tttgtcccat tttttagttg aaattttttt
     6301 aggacaattg gtttggaccg cctttttttg ctcatccaaa tttatttttg ggctcttttc
     6361 catagtataa ttgtatttgg tttcaaaatt aaatccattc taaatgaatt ccttacggaa
     6421 aaccgtaaac tgaaaaataa attaatctac atcataataa taatggcaat acacattatt
     6481 aaaataatga gtttcaattt ctggagcttt cattaatgct acaaattcgt ctagggttac
     6541 gccaaaatac tggtgaatta cttgaagttt aaattctatt tcaagggttt tatgaacttc
     6601 gtcatagcct acagagatga tgttgcggtc gtttacggta tatcgattca ttgtttttat
     6661 attttttttc aaatgtaaat ccaataaaaa agacattttt acggaaaacc gtaaaacgag
     6721 aaaataattt aaaaaaaaat tgaaaatgga atgaacctag gaaagtaaaa aaactaaact
     6781 tctgtattaa cttaggtgtt tttggaaatt acccccttaa acttttaacc ttaaacctta
     6841 tactccacaa tcacaccaat cctaaatctt tagcaatagc aatgagatgt acattattat
     6901 tggctttgaa gaatattttg agcttgctga ttcgtttctc cagactgcta ccaccattgg
     6961 gaattattcc tgagtttttg aaatctaatc cgatttcatc tacggttaat cctttagaca
     7021 atgactttag cagtaaaata tcatagtttt cgatttcaaa aagcgatttg tctctcaagg
     7081 cataggacaa ttcagttgaa acaatttttt cgtcagtatt ataaacccgt tgaatggctt
     7141 tttgaagttc gggaatgcta tttcgacctt tagaaacata ggcatttatt tctaaatttt
     7201 cgaacaagga tttgattcta aaggatttgt cttctataga aaacactatg gtttttatat
     7261 ggggttgtat ttttttgact gcttcaataa gttcatcacc cgaaccgagt tggttgtctc
     7321 gatggtctaa tttgaaagat aaatcgctta tcagtaagtc gtaagggcta ttgtcatgca
     7381 aacctttttt gatttttagt aaggcatcat cacaatattt ggcgtggtgt atttctgtaa
     7441 tgccaaggtc ttcgatggct tgaacaacag caatactgat ggagtctaaa tcttcggcaa
     7501 ctaacacttt ttgaaacata aaaaaacgga ttatattgga attgaaaaac tgactttgaa
     7561 tcctttattg gatttagtgt caaaagtaat cgttccgttt tgagctagaa tacggttttc
     7621 cacattttgg agtccatttt tgaaatttat ttcgtttaac gacgctccaa gcccgttatc
     7681 actgtaattg atgtgtaaaa aattttcttc tttttttaaa gaaatggcaa ctaaactgca
     7741 tttgctatgc tttttcatat tcactaatag ctcttgtagc actctataaa ttgttatttt
     7801 tttagtgttt tctaataaag tccaatcaaa gtcatccaat ccgtttatta atatattgat
     7861 ttccgaagta ttaaaacgag ccatcatttc tttgagattg ggtacaaagt tttcgcctgt
     7921 ttcgatagca ctattttctt tagaaatatt tcgagttcgg gtataaatag tatcgagatt
     7981 gttcaataag gtttctttgt tttgagcaga tgccaaatct tgtgtttcgg caaaagctat
     8041 agtttgaaag acatcgttag ccagctcgtc gtggagtttt ttggcaattc gagtttcggt
     8101 ctcgtaggag gtttggattt tttctttttt gttaatcgct tttaaaaaat aatataaaaa
     8161 tattgttgac aaaataccta ttgcgataag aaaacgcaaa agtagatttt tgtttttttg
     8221 tatttctaat tcaagtaaat tttcggattt ctgatttttt aataacaaat tttcttcctt
     8281 ttctttcttg gaatcatatt ttattttggc aaactggttt ctggcatttt gtcttactct
     8341 gttgatgctg tcattgagat gtatgtattt ttctgaatag aatttcgatt gatttccaga
     8401 gctggattgg attaataatg ctaaagcttc caaacggtca tcaatattat tcacttttgt
     8461 agctttttcg aatgccaatc gagcataaac attcgctaat ttggggttgg ttggtttgta
     8521 atattgggtg aggtgaagat agcttgccgc cattccccag tcatctttta atttttcttt
     8581 tattttcaat gcctgattta aataatcaat tcctttggga ttccccactt taaagtaaga
     8641 aaaacctaaa ttatccagtg cttttgaata gggagcagga tcatttataa cttctttttt
     8701 taatgtcaat ggaagcagaa tttgtatggc ctgattgtaa tcgtgcttag ccatataaac
     8761 cacagcaata ttgtgctgga tttttaattt tcgggtttta tcaattttta aattcaatcc
     8821 ctttttgtaa taataaaggg agttatcgta atcgaaaagt ttcaaataga ctactcctag
     8881 taaattataa atagaactct cgtaggaagg atccatggtt tcgtttacaa aagagagagc
     8941 ttcaatagct gttgtttcac tacctgaata atctccttga ttttgttgaa tttctgacat
     9001 tcttgccaaa gaatagataa ttcgggtatt gtcagtcttt acatcacaga gcgatttggc
     9061 tttgttgtga taatagtaag cactgtcata attcactttt tcaagatatt ggtcgccaag
     9121 gtcaataaga cgatcaattt ctgctaaatt tttctctttt ttgggattga ttattggttt
     9181 cttgtcgcaa gattgcagaa gaagaaatag ggttgctaaa accaaaatcg gggaatgtaa
     9241 ctttttgtga atcatccccc gaaattacta aaattattca aaacagataa ttaatctgac
     9301 taataaatta cttttagttt aggcagtgta ataggatttt gtcttagttt aattttcact
     9361 gttttatata tcagcttggt tttagtttat ttccaatcaa atacaatgaa aatggttttt
     9421 gtgtttccaa cattatttgt tattttcaat cacataattc aaaaccttag tcatcaaatg
     9481 cactctgtct ttgccaataa cattgtgctc gtgcattgga taagcaaaga aatctacttg
     9541 tttttcggct tcaataaatt ttttcagtaa tacaaaattg ttttgcataa ccacagtatc
     9601 atcactagtt ccgtgaatca gaagtaattt tcctttcagg ttttttacat aattcaaagt
     9661 acttgcttcg tcaaaacctt tttggttttc ggcgggggtg tccatatagc gttcaccata
     9721 catcacttca taatagttcc aatcggttac tggtccacca gcgattccta ctttaaaaac
     9781 atctggtttt cgaagcatga tagaagtagt catgaatcca ccaaaactcc aaccgtgaat
     9841 agccaaacgg ttttcatcta cataaggcaa ggatttcaaa taatcaacac cttttatttg
     9901 gtcttcgatt tcatttacac ccaatcttcc gtgaatcaca ctttcgaatg caaaacctcg
     9961 attatctgaa cctcgattat caaccgtaaa tattaaatag ccttgctctg ccatccaata
    10021 catccaaaga ttggcaccat ctaaatagga attagttatc aattgagcgt gaggaccacc
    10081 gtaaacataa acaagtacgg ggtatttttt tgtcggatca aaattgctgg gtttgattaa
    10141 tctagtatat aaatcagtag ttccatcggc cgattttatg gttttgattt cggcagttcc
    10201 catttggaaa tcggcatatt tgttgctgct ttctaaaagt gttttggcgc taccgttttt
    10261 gctatacagc agcgattttg atggggttga atgattggaa tattcatcaa aaaaataact
    10321 tccatcaaaa tttggaatgc aattatgaac gccttggtct ttggtaatta aagtttgctt
    10381 gccattcaaa tctactttgt aggccaacat attggtaggg ttttcgccag ttgctttaaa
    10441 gtaaatttca tttcctgcag tattgcttcc aattatttct ctcacaggaa atttgttttg
    10501 agtcaattgt ttgattaatc tcccttcaat cgaataataa tacaagttgt tgaaaccgct
    10561 tttttcgctt atccaaacaa aattattgtt ggcattgctc ggaaaaaaag caggatgttc
    10621 tggttcgacc caattgtcat ttttttcatt aagaatagtt cgaacaaaat ttcccgtttg
    10681 ggcatcataa acgtttaatg acatatcatt ttgcgctcga ttgagttcag caatcaaaac
    10741 atatttttcg tctggcgacc aagaaagatt ggttagataa tcatcaaggt ttccttttgg
    10801 tgaaataaaa actgttttct tactagcgag attgtaaatt cctactcttg gtttttcgct
    10861 cttttgacca atcattggat atttgatatt gactaatttt cctggagttt cgttaatgtc
    10921 taatatagga taatcagcaa cttcagtttg gtctttttgg taaaaagcta aaaagttaga
    10981 ttttggcgac caaaaaattc cgtctcttat tccaaattca cttctagcaa aaaattgacc
    11041 agaaacaata gcctcgttgg tttcattggt gacttgaact atttctttat tgacgttgta
    11101 gtaataaaga ttgttgtttt ctgtaaaggc aagatttctc ttttttatat cataagttag
    11161 attttggcaa ctgcttttcg gttcttgcat caacgtccct ttttttgaag tcgttaaata
    11221 ggcgtattgt tttccgcttt cagaaacgag tagggcattt gaatcaatcc attgtatgcc
    11281 aaaaaagttt ttcaattttg ttcctaaggc ggaattaata tcagctaaag ttaccgtttc
    11341 aatagtattt gaatctgtgg cagaggcagc aaccattttg gtgtaattat ctgtataata
    11401 ggtataatta gtggtgttcg gaatccactg aaatccaatt aatttatcgg ctctgaagag
    11461 tctgttttgt tctaaaaccc cttgttctag agtcagtttt ttggtttgag aaaaggtagc
    11521 aattgttata aagcaactta gagccagaat agaaaaattc ttcatatatg taggtttttt
    11581 gatagggaat aaaagtacaa aaatgatttt accaaataat tgtatcagtc taaaattaaa
    11641 aaattttgtt acagaagata ccttttttca atattaattc aggaaaaatt caaaccaaat
    11701 aggaatatgg tctgaaatca ctctcgcttc ctttagagaa ttgaaatttt tgtaaaaagg
    11761 aagaactcct gaattgagtc tagcaatttt cgaagaatta taaaacatat tgtcaaattc
    11821 cgaagcgaga caagtatcat attcacattt ttgtttcaaa gacgttttct gattgacaag
    11881 aatagattga tatcccatct ttttcaaagg attaaaaacc gtgtgtgatt gcggacagtt
    11941 gaaatcgccc acaaagatca aattcagatg gggatattcc tcaggcagga atttaaaata
    12001 tttaatctcc gtttcgggtt gcattttctt agtgatggca tgaaagttga ctatcgtaaa
    12061 ttgtttgttc tcatattgaa acgtacagaa ataaggttct cggtcaatct ccagatgata
    12121 ttttttttcg agccacgctc ttccaatttt tttaatttta ctcgttttcc aaagaaaagc
    12181 ataccgttcg gttttgtaag cactactact cgttggttcg ctgatggtgt aatcccattt
    12241 tgctcctttc cggttcagtt catccgcaag tttggctaca gtttgcgctc caccatcgcc
    12301 cgccacgact tcctgaatgg caacaatatc atattctctc aaagtattgg caataaaagt
    12361 gatttcagtg tttgatttcg agctaccaaa gttttctaga ttccatgaaa ggagtttggg
    12421 ttgggagaat agggtggtgg tgaataggaa aaggaatagg attatttgtt tcatttacta
    12481 ttttttgatg tctttgcgaa tggctaaaga tagtaataaa ccaactccaa atattacatc
    12541 gacaacattc cggaggtttc tgcctaaggc aatttttaaa aaaggttgaa attaaatggc
    12601 taaagtaatg tataggaata cttcgttttt gttttttgta ctgttaagag aaaatgctaa
    12661 atatgcgaaa cctagcatta cagtatatct tacaaattgg taatatccat atggcgttta
    12721 aatagacata tttataagtc tacataatac ttcatagtct tttaatttaa aaattattta
    12781 tcctccaaac ctacaaccct aaaaattctc ttctttacgg gaaacctcat tttgagtatt
    12841 ttttttacta ttcatcacat taaatgttct ggaggatgaa ttattttgta tgaaacagta
    12901 ctatttacca gcaaattttt gaaatccgta ttggattaaa tattcattgt agttattaac
    12961 tgtttttttg ctatcgtcat tcaattgatt ttggataaaa ggaatagcct tatcagaaat
    13021 agattcattg acggcatccg gcaaaaacgg actcttgcta aatgttgttt tgcagcccaa
    13081 cgaatctgga ttcattgtta catctaacga agctttattc actcttccaa caaagttgaa
    13141 tggaatcgac gaacaaatta ctgctatcga aggagaatgg aaagtctttt ttgatccaaa
    13201 atggggagga cctggtgaag ttttttttac taacttaacg gactggacca agaatgtgga
    13261 tgaccgaata aattactatt ctggaacatc ggtatatcga aagtcaatta aattggatca
    13321 acccatttcg ggtacgcagg tattacttcg attcccacaa ttaggttcta ttgcgcgcgt
    13381 gttgattaat ggaaaggaag tttcgaccgt ttggtgtagt ccatgggaag cagatataac
    13441 gctttacata caaaaagggc aaagaatacc ttcaattcat gaccttacag gaaatttatt
    13501 gctggaaaca aagaagaatt gatagaggat gttgctataa aagagtttgt aaaaaaagag
    13561 gaaagggtaa aaaaagagga taaaaaggag gagattgttt ttcaagaatt gaataagaag
    13621 agtgtttcaa agaaaatagc ttttgcggtg attcctttgt ttgtttttgg tgctgcgggt
    13681 tttggcatca aagacatttt ttttcgagac aagaattgta tggtttgggt caaaaaccat
    13741 tatgaagcag ctgaatgtga tgcaataaaa gatacggccg aagtttgtcc gttcaatcaa
    13801 gggatattgg acaatttcaa aaaaatatcg gtttgtgata cgactacttt tttcaaaaat
    13861 ggagatactg acaatcctct tgtttggtat ggaaaatctc cggataaaaa agaatatgaa
    13921 tatttcaatc aaccaggatt gcatcccgaa acgggtaaaa cattgaaacc aatatcgaaa
    13981 tatattattg gaaaatacat tttaaaaaag aacgaatagc aattagaaat agtaaagtct
    14041 agttgttttt ttgtcacatc gagcgcagtc gagatgctct aatggttatc gactgaattc
    14101 gaattgacaa taagaccaat ttataaatgg tatagaatct attttcatga gtcctacaaa
    14161 aacaaaaaac ccaatctttc gactgggttt ttctataagt aagtaatctt aaattgagtt
    14221 gataaaacgt ttattttact ttattaagta tggctttgaa agctactgga tgattcatcg
    14281 ctaaatcggc aagaacttta cggttcaatt cgatgttatt agctttgcat tttccaatga
    14341 attgcgaata agacattcct tccaatctgg ctccagcgtt gatacgttga atccacaaag
    14401 cgcggaagtt tcttttgttc gcttttctgt cacggtaagc atagcacatt gctttctcaa
    14461 ccgcgttttt cgcaactgtc caaacgtttt tacgacgacc aaagaaacct ttggcttgct
    14521 tcattatctt ttttcttctt gctct
//
LOCUS       DAOSNN010000034        14430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_4214_length_14430_cov_5.581217, whole genome shotgun sequence.
ACCESSION   DAOSNN010000034 DAOSNN010000000
VERSION     DAOSNN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14430
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4214_length_14430_cov_5.581217"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..225
                     /gene="atpE"
                     /locus_tag="PLQ70_03155"
     CDS             31..225
                     /gene="atpE"
                     /locus_tag="PLQ70_03155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HQE33642.1"
                     /translation="MEIPKIIGAGLVVIGAGIGIGRIGGSAMDAIARQPEATGKIQTA
                     MLIAAALIEGIGFAALFAVN"
     gene            316..816
                     /locus_tag="PLQ70_03160"
     CDS             316..816
                     /locus_tag="PLQ70_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HQE33643.1"
                     /translation="MEKLWEDFGFGLFFWNAIIFVGLIFLLKKFAWKPILDAVNEREE
                     GIKNALLSADNARKEMQNLQSDNQRILQEARLERDALLKDARDMKEKMVADAKNEAQA
                     QGQKMIEQAKAAIESEKNAAVAELKLQVSTLSLQIAEKLLKDELSNKEAQVKLVEKML
                     GDAKLN"
     gene            818..1354
                     /gene="atpH"
                     /locus_tag="PLQ70_03165"
     CDS             818..1354
                     /gene="atpH"
                     /locus_tag="PLQ70_03165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HQE33644.1"
                     /translation="MASTRAAIRYAKAILEIANSKNATSEVSADMALIASTIQSNSEL
                     NSFIQNPLVRTENKKDVVSEVFASVNPVTQSIFQLLLENKRFGILDSIAVEYNKLFDI
                     MNGVETAKVTTAVPMDAALEAKVLAKIATISSSKKITIENIVDPAIIGGFILRIGDKQ
                     YNASIADRLQVLKRELSN"
     gene            1371..2948
                     /gene="atpA"
                     /locus_tag="PLQ70_03170"
     CDS             1371..2948
                     /gene="atpA"
                     /locus_tag="PLQ70_03170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQE33645.1"
                     /translation="MAEIKPAEISAILRKQVEGFESGATLEEVGSVLQVGDGIALVYG
                     LSNVQYGELVEFENGLEAIVLNLEEDNVGVVLLGPSTGIKEGSTVKRTQRIASLKTGE
                     GMVGRVVNTLGQPIDGKGPIGGELFEMPLERKAPGVIFRQPVTEPLQTGIKAVDAMIP
                     VGRGQRELVIGDRQTGKSTVCIDTILNQKEFYDAGKPVFCIYVAIGQKASTVAGIAKM
                     LEEKGAMAYTVIVAANASDPAPMQVYAPMAGAAIGEYFRDSGRPALIVYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERACKVIADDSIAKDMNDLPDSLKGIVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLDGDLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQFRELEAFAKFGSDLDAVTLNVIEKGKRNVEILKQNLN
                     DPYTVENQTAIIYAGSKNLLRNVPVDKVKEFEKDYLEFLNNKNRATLDALKAGKLTDE
                     ITDVLEAAAKEVSAKYN"
     gene            3034..3894
                     /gene="atpG"
                     /locus_tag="PLQ70_03175"
     CDS             3034..3894
                     /gene="atpG"
                     /locus_tag="PLQ70_03175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HQE33646.1"
                     /translation="MANLKEIRNRITSISSTMQITAAMKMVSAAKLKKAQDAITAMRP
                     YAEKLTELLQNLSATLDGDAGGEFTKQRELKKILVVAITSNRGLCGAFNTNVIKEVKN
                     RSDFYAGKQVDVFAIGKKGNDLLSKTHTVVDNQSQVFDALTFENVTVIAEVLTQKFVS
                     GEYDKIELVYNQFKNAATQIVQVEQFLPLAPIQSDKPASTGDYIFEPSKEEIVLTLIP
                     KSLKTQLYKGVRDSFASEHGARMTAMHKATDNATELRDQLKLTYNKARQAAITNEILE
                     IVGGAEALKG"
     gene            4139..7144
                     /locus_tag="PLQ70_03180"
     CDS             4139..7144
                     /locus_tag="PLQ70_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE33647.1"
                     /translation="MKLSNLIGFLFLATSVYSQNAIVAELSDNLVADATIHTNIDDAS
                     IIKGTVIEESTGKPLPGVLVVIKDTKFSTITDAEGKFYFRKMPAGKYNLEFSFFSFST
                     KIVSDVEVIDKEATVLTVSLAEMNGTLDEVIVKTTVKAKAESVQSLLTMQKTSVRVSD
                     GISAESIKRTPDKTASDALRRISGASIQNNKFIVIRGLNDRYNTTYLNGSPLPSTEPD
                     RKAFSFDIFPTNMLDNLIINKTASPDLPGEFAGGVIEINTKMAPDKDFQTLSIGGGYN
                     TVTTGKTQIKANDAKVGLPSYFPSSAVMFELQKPPKTESNILAIDDLSKKYQTDWGTK
                     EDQFDPNTSFQLSLGRYFKFNGDQSLGLLASVTNNVSNVYADRTQKFYEVANDLREDF
                     SDKSYTTQRLSGAILNLTLKMNTNNKFSFKNLYSVNTETRFTDRFGTKNVTDVEPIML
                     ISTNRFFSRNNIYTGQLIGEHFLPDSKIKINWVGSYSNIKRDIPSERRNTYEYIQFSD
                     GTQSVPTANFIIGVVGKDSPGSIFTSLNKEFIFSTKIDVSKKINFSDDFSADFKIGGI
                     TQSRNRKFEARQLGYIPFNGTVGGVRYETVSPSITVLPSETMFNSSNMGILSTSPKVS
                     GLTLYDGTKGSDTYDAESNLDAGYLMIDNVFKKLRVVWGVRVENYYQFLNSKTIENTP
                     ITVEDKQFDVLPSINFIYKLTKNQNIRLSGSKTLNRPEFRELAPFLFFDNETRFNTSG
                     NPELKITEIYNADLRYEFFPGKGQLFSVSAFYKDFKNPIELQALANNSNIYKNAKSGT
                     DYGVEFEFRSLLSTMTGMKERKFFDDLTLYANLAVIRSKVDISNLFETPYAIEDTPMQ
                     GQSPFVLNAGILYTNTEAGWSFSANLNRIGDRISIHANQTLGNPVAGFWEKARTLLDL
                     QLAKTLFNKKLEFKINLQNALAQDLILYQNNYELSSTANQVKGFKAFTNKMILGDSQN
                     KNGYDSSYDDLNARIKFGSTYSFTLTYSF"
     gene            7297..8925
                     /locus_tag="PLQ70_03185"
     CDS             7297..8925
                     /locus_tag="PLQ70_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33648.1"
                     /translation="MKKNYLFSMLLLVTFTGFSQIITTTYRGAFAPAPAAMWTDIWTE
                     YDPQNKVYPTPTVTISTDITTNTTWTAGNTYSLGGQIYVKNNATLTIEPGVIIRSTAP
                     GAGLFITKGAKLMAEGTAASPIVFTSGYSEGNRSRGDWGGVILMGKGSYNINGGVNNI
                     EGIAPTTDTQYGGGATPDDNDNSGSLKYVRIEFAGYTYGAAGSNTEINGLTMGAVGKG
                     TTIDYVQVSYANDDSFEWFGGAVNCSHLVAYRGLDDDFDTDNGFSGSVQFCLAIKEPA
                     IADSSDSNCFESDNNSTSTAGTSFTRAIFSNCTLIGPSYRATLPGGGALAIRHRRGAH
                     LRRNTQLQVYNSIFMDFVDGLVVDGTTTITNATNNTLKFKSNIIAGTAAAKVAVKVES
                     PATTFDVATWFTNNNNTSQTASAGLLTLPYNTTDGSLYTGLDYRPAVGSIALSGADFT
                     NLGTDKFVSNAEFSLEVYPNPVVNSFKLNYSSSSNEDVKVATYDITGKLVESINVKYA
                     DINDQEMGNNYNAGVYILVLKQGDINKSVKVIKK"
     gene            9117..10580
                     /locus_tag="PLQ70_03190"
     CDS             9117..10580
                     /locus_tag="PLQ70_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQE33649.1"
                     /translation="MGLYKNLFKQTAIYGLATVLPRMLSFLLVRLYTGILPTTDYGEV
                     SILLSWMVFFNVILSYGMETGFFRFYNSETDKQNVIATSTISIFWSTIFFLFGALIFR
                     NSLASFANVDVQYITYSIWILALDALVIIPFSKLRANQRPIMYAVIKIGNVIVNLILN
                     IFFLVFLPQLAADNPHSFFDNLYVEHSEIGYIFIANLLASLLTLVVLSPNYFSLNRKF
                     DKELWKKMMKYGLPILVAGIAFAVNEHFDKILLGYLLPENIAKSEVGAYSACYKLGLF
                     MVLFATAFRLGIEPFFFSHSSSENAPQTYAVITKYFVILGSLILLGVIVFADVLKYLL
                     LDNKSYWEAMKVVPLIILANFCLGIYNNLSVWYKLTDKTHIGAYISIVGAILTLVLNY
                     LLIPKYSYYGSAIATIAAYGSMMIISYVLGNKYYPIPYDMEKIGGYLGLSILFSVIYF
                     YGFRENYFVGIPLLLAFMYFVYHNEKATILGILKRKK"
     gene            10589..11023
                     /gene="dut"
                     /locus_tag="PLQ70_03195"
     CDS             10589..11023
                     /gene="dut"
                     /locus_tag="PLQ70_03195"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQE33650.1"
                     /translation="MKINIINKSQHDLPSYETIASAGMDLRANLTESITLKPLERTIV
                     KTGLFIELPIGYEAQVRPRSGLAAKKGITVLNSPGTVDADYRGEIGVILVNLSNEVFV
                     VENGERIAQLIIAKHERAEWIEVQELSETSRGEGGFGSTGVK"
     gene            11131..12141
                     /locus_tag="PLQ70_03200"
     CDS             11131..12141
                     /locus_tag="PLQ70_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQE33651.1"
                     /translation="MKIIVPMAGRGSRLRPHTLTIPKPLIPIAGKPIVHRLVEDIAGV
                     LNQDIEEIAFIIHESFGKKVEEDLIAIAEKLGSKGTIYYQNEALGTGHAIMCAKDSLS
                     GPAVIAYADTLIRADFDLDQNADSVIWVKKVDQPEAFGVVNLNEKNQIVELVEKPKEF
                     VSDLAVIGIYYFKDVAVLKNELQLVLDNNIINGGEYQINDGIKQMMAKGMIFVPGEVA
                     EWMDCGNKDVTVETNSRMLGFLNQDGEELVAKNVKLDNSTIIPPCYIGENVILINATV
                     GPNVSLGNACHVSNSSIKNSLVQTHSHIKNANLDNAMIGSHASFDGNFTSISIGDYSV
                     LE"
     gene            12228..13577
                     /locus_tag="PLQ70_03205"
     CDS             12228..13577
                     /locus_tag="PLQ70_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE33652.1"
                     /translation="MKKSVSIFLFLVLLCNSASLLAQTEPEEIKTETDQFEEHFYESL
                     KQKGIENYDKAITELGYCLKLKPNEASVHSEMGRNYLASKQYENAYSSFDKATQIDAK
                     NKWFWIGMYDVCYQTKNYNQCIVVVNKLIEFDPIYKDDLVSLYMNTQQFDKALVLINE
                     LNEKVGKSDMRDLYKAQILSQGKFQNAEIDNLLAQIKLNPKVESNYVALIKLYSEKNE
                     AQKAVEITKKLEAEIPESEWTQVGLFKFYLDKKEGPNAIKAMNIVLASSKIDSKIKHR
                     VLNEFLNFVNTNPQYSPDLEKAISYFDKDSDVKVAKEIGKYYHAKKQWDKAITYYELS
                     LKNSSNEDVETNLLLLQAYVETKQFEIVKKKASSLVESYPTQPQFYYFSGLANNQLKE
                     YKKAATMLEVGIDFVVEDVILEANFNLQLGKAYNGLGDFKKKEMYFSKANQLLRAKK"
     gene            13586..14383
                     /locus_tag="PLQ70_03210"
     CDS             13586..14383
                     /locus_tag="PLQ70_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HQE33653.1"
                     /translation="MNKFFKNCKTFFLVSFISSLTLVSCKSKTAAVDVSKTENKKSIN
                     KIIENYYNNKTDFSTLYIKASVQYASEKQNQNVTAEIKLKKDEQILISIRFLGITMAK
                     ASITPTKVSYYEKVKSTYYEGDFSALSQLLGTDLDFSKVQNMLLGRAIDNLKEGKYTE
                     SLENQVYRLDETADDNLKKSFYIDANSFLMNKQEITQTKEERMIQVVYSNPKEFKEMT
                     LPLNINIDTYQKKGKAEINLEYNTVSFNEELSFPYSVPNDYKRIIIK"
BASE COUNT         5013 a         2298 c         2707 g         4412 t
ORIGIN      
        1 ttgaatgttt aatttttaat acatattttt atggaaattc ctaaaatcat tggagcagga
       61 ttagtagtta ttggagcagg tattggtatt ggtagaatcg gtggttctgc aatggatgct
      121 atcgctcgtc aaccagaagc tactggaaaa attcaaacag ctatgcttat tgcagctgcg
      181 cttattgaag gtattggttt cgcagctttg tttgcagtaa actaaaaaag acaaaaagct
      241 gtaacggttg gttgcagctt ttgttttaat tttaaaaaca cacattgtta caatataaat
      301 tatacttaaa ttaagatgga aaaattgtgg gaagatttcg ggtttggatt gtttttttgg
      361 aacgccatta ttttcgtagg attaattttc ttattgaaaa aatttgcttg gaaaccaatt
      421 ttggatgctg ttaacgaaag agaagaagga ataaaaaatg ccttgctttc tgccgacaac
      481 gctagaaaag aaatgcaaaa tcttcaatca gacaatcaac gtattttgca agaagcaaga
      541 ttagagcgtg atgccttatt gaaagatgct cgcgatatga aagaaaaaat ggttgccgat
      601 gctaaaaatg aagctcaagc tcaaggtcaa aaaatgatcg aacaagccaa agctgctatc
      661 gaaagcgaga aaaatgctgc cgtggccgaa ttgaaacttc aagtttctac tttgtcactt
      721 caaatagccg aaaaattatt aaaagatgaa ttatctaaca aagaagctca agtaaaattg
      781 gtggaaaaaa tgttaggcga tgctaaatta aactaatatg gcaagtacaa gagcagcaat
      841 tcgttatgca aaagccattt tagaaatagc aaattcaaaa aatgctactt ctgaggtaag
      901 tgccgatatg gcattgattg catcaacaat acaatcgaat tcagaattga attcttttat
      961 ccaaaatccg cttgtaagaa cggagaataa aaaggatgtt gtttcagaag tttttgcttc
     1021 tgttaatcca gttacccaaa gtatttttca attgttattg gaaaacaaaa gattcggaat
     1081 tttggatagc attgcagtag aatacaacaa attgtttgat attatgaatg gtgttgaaac
     1141 agcaaaagtt actacagccg ttccgatgga tgccgcatta gaagcaaaag ttttggctaa
     1201 aattgcaacg atttcatcaa gcaaaaaaat tacaattgaa aacatagtag atcctgctat
     1261 tattggagga tttattttaa gaataggcga taagcaatat aatgcttcta ttgccgacag
     1321 attacaagta ttaaaaagag aattaagtaa ctagttattt ataaataatt atggcggaaa
     1381 ttaaacctgc tgagatttca gcaatattaa gaaagcaagt agaaggtttt gaatctggtg
     1441 ctacattaga agaagtaggt tctgtacttc aagttggaga tggtattgct cttgtttatg
     1501 ggctatcaaa cgttcaatac ggtgaattag ttgaattcga aaacggattg gaagcaatcg
     1561 ttttgaatct tgaagaagat aatgttggtg tggtactttt aggaccatca acaggaatca
     1621 aagaaggttc aactgtaaaa agaacacaac gtatcgcctc tcttaaaact ggagaaggaa
     1681 tggtaggacg tgtagtaaac actttaggtc aaccaattga tggtaaagga ccaatcggtg
     1741 gtgaattatt cgaaatgcca ttagaaagaa aagctcctgg agttatcttc cgtcagccag
     1801 ttaccgagcc attgcaaaca ggtatcaaag cagtagatgc gatgatccca gtaggtcgtg
     1861 gacaacgtga gctagttatt ggtgaccgtc aaacaggtaa atctaccgtt tgtatcgata
     1921 ctatcttgaa tcaaaaagaa ttttatgatg caggaaaacc tgtattttgt atctatgttg
     1981 ccattggaca aaaagcttca acggtagcag gaatcgcaaa aatgttggaa gaaaaaggtg
     2041 caatggctta taccgttatt gttgcagcta atgcttctga tccagctcca atgcaagtat
     2101 atgctcctat ggcaggtgct gcaattggag aatattttag agattcaggt cgtccagctt
     2161 tgattgttta tgatgattta tctaaacaag ccgttgctta ccgtgaggtt tctcttttat
     2221 taagaagacc accgggacgt gaggcatatc ctggagacgt tttctactta cactctcgtt
     2281 tattggaaag agcttgtaaa gtaattgctg atgacagtat cgcaaaagat atgaacgatt
     2341 taccagattc tttaaaaggt atcgtaaaag gtggtggttc attgactgct ttgccaatta
     2401 tcgaaactca ggctggtgac gtttctgcat acatcccaac taacgtaatt tctattactg
     2461 atggtcagat tttcttggat ggagatttgt ttaactcagg ggttcgtcca gcgattaacg
     2521 taggtatttc tgtatctcgt gttggaggta atgctcaaat taaatcaatg aaaaaagtag
     2581 ctggtacatt aaaattagac caagcacaat tccgtgaatt ggaagcgttt gctaaatttg
     2641 gttctgattt ggatgccgtt actttgaacg taattgaaaa aggaaaaaga aacgtagaga
     2701 ttttgaaaca aaatctaaac gacccttata cagttgaaaa ccaaactgcc atcatctatg
     2761 caggttctaa aaacttgttg agaaacgttc ctgttgataa agtaaaagaa ttcgaaaaag
     2821 attacttaga attcttgaac aacaaaaaca gagctactct tgatgcatta aaagcaggta
     2881 aactgactga cgaaatcaca gacgtgttag aagcagcagc taaagaagtt tcagcgaaat
     2941 ataactaatt aggttctagg ctttaggttt tgggttatag ttaattctaa taaaccaaga
     3001 cctaaaacca aacaacccaa tacccataat aaaatggcaa atttaaagga aatccgtaat
     3061 agaattactt ccatttcatc gacgatgcag attactgctg cgatgaaaat ggtttctgca
     3121 gcaaagttga agaaggctca agacgccatc acagccatgc gaccttatgc cgaaaaacta
     3181 acggaattat tgcaaaatct ttcggcaact cttgatggag atgcaggagg agaattcacc
     3241 aaacaacgtg aacttaaaaa aatattggtt gtagcgatca cttctaatag aggtttgtgt
     3301 ggcgctttta ataccaatgt aatcaaagaa gttaaaaacc gttccgattt ttatgctgga
     3361 aaacaagttg atgtttttgc cattggtaaa aaaggaaatg atttattgag caaaacacac
     3421 actgttgttg acaatcaaag tcaggttttt gatgcattaa ctttcgaaaa tgttaccgtt
     3481 attgctgaag tattgactca aaaatttgtt tctggagagt atgacaaaat cgaattggtt
     3541 tacaatcaat ttaaaaatgc agcgactcaa atcgttcaag ttgaacaatt tttaccgtta
     3601 gctccaatac aatcagataa gccagcttca acaggtgatt atatttttga accatcaaaa
     3661 gaagaaatcg tgttgacctt gattccaaaa tcattgaaaa cacaattata caaaggagtt
     3721 agagattcat ttgcatctga acacggagcg cgtatgactg ccatgcacaa agcaacagac
     3781 aacgcaaccg aattgagaga tcaattgaaa ttgacttaca acaaagcccg tcaagcggcg
     3841 attactaacg aaatcttaga gattgttggt ggagcagaag ctttgaaagg ataattattt
     3901 atttaatata catttaaaag tcccgcaatt tgtgggactt tttttttgat ttaaagacag
     3961 aaaatttaca taatatttaa tttagaaaga aatcaacata tacttatcgt ttatctaacc
     4021 ataacttaac gttggatgaa ttttagagaa atttgttaat ctatcagtaa cattcaaaat
     4081 ttagtttcaa agtatttttg ccgtgaaatt ctagcaattt ttaatcaaaa atagttcaat
     4141 gaaattatca aaccttatcg gatttttatt tttagccact tctgtttatt cacaaaatgc
     4201 tatcgtagct gaattatcag acaatcttgt agcagatgcg acaattcaca ccaatataga
     4261 tgatgcttct attataaaag gaaccgtaat cgaagaatct acaggaaaac ctttgccagg
     4321 cgttctggtt gtaataaaag acactaaatt ttctactatt accgatgctg aaggtaaatt
     4381 ttattttaga aaaatgccag ctggtaaata caatttagag ttttcgtttt tctcattttc
     4441 aaccaaaatt gtttccgatg ttgaagtgat agataaagaa gccacagtat taacggtttc
     4501 tttggctgaa atgaacggta cattagacga agttattgta aaaacaaccg ttaaagcaaa
     4561 ggcagaatca gtacagtcgc ttttaacaat gcaaaaaacc agtgttcgag tttctgacgg
     4621 aatttcggcc gaaagcatca aaaggacacc agacaaaaca gcctctgatg cattaagacg
     4681 tattagtggg gccagtattc aaaacaataa attcattgta atacgaggtt taaatgaccg
     4741 atacaacacc acttatttga acggttcgcc attgcctagt acagaacccg ataggaaagc
     4801 tttttctttt gatatttttc caaccaatat gctcgataat ttaattatta ataaaacggc
     4861 atctcccgat ttgcctggag agtttgcagg aggagttatt gagattaaca ctaaaatggc
     4921 accagataaa gattttcaaa ctctgtctat cggtggggga tacaatacag tgaccaccgg
     4981 taaaacacaa attaaagcaa atgatgccaa agtgggatta ccttcttatt ttccgagttc
     5041 agcagttatg tttgaattac aaaaacctcc taaaacagag agcaatattt tggccattga
     5101 tgatttgtct aaaaaatacc aaacggattg gggaactaaa gaagatcaat tcgacccaaa
     5161 tactagtttt cagttaagtc ttggtcgtta ttttaaattc aatggagacc aaagtctagg
     5221 tttattagct tctgtaacga acaatgtatc taatgtttat gcagatagaa ctcagaaatt
     5281 ttatgaagtt gcaaatgact taagagaaga tttttcagat aaaagttata ctacacaaag
     5341 acttagtggt gctattttga atcttacttt aaaaatgaat actaacaata aatttagttt
     5401 caaaaattta tatagtgtca ataccgaaac tagatttacg gatagatttg gtactaaaaa
     5461 cgtaacagat gttgagccaa taatgttgat ttcgaccaac cgattttttt ctaggaacaa
     5521 tatttatact ggtcaactta taggagagca ttttttacct gacagcaaaa ttaaaattaa
     5581 ctgggtaggt tcctatagca atataaaaag ggatattcca agcgaaagaa gaaatactta
     5641 tgaatatatt caattttcag atggaaccca atcagtacca acagccaact ttattattgg
     5701 tgttgtaggt aaggatagtc caggttctat ttttacatca ttaaacaaag aatttatctt
     5761 tagtactaaa attgatgtga gtaagaagat aaatttttca gatgattttt cagccgattt
     5821 taaaataggg ggtattactc aatctcgaaa ccgaaaattt gaagctcgcc aattgggtta
     5881 cattcctttt aatggaacag ttggaggagt aagatatgaa actgtttcgc catcaattac
     5941 ggtattgcca agtgaaacta tgtttaattc ttcgaatatg gggattttgt caacttcacc
     6001 aaaagtaagt ggattgactc tttatgacgg tacaaaagga agtgacactt atgatgcaga
     6061 gtcaaatttg gatgccggtt atttgatgat tgataatgtg tttaaaaaac taagagtggt
     6121 ttggggtgtt cgtgtagaga attattatca atttttgaat tctaaaacta ttgaaaacac
     6181 accaataact gttgaagaca aacaatttga tgtgttgcct tctataaact ttatttataa
     6241 actcactaaa aatcaaaata taagattaag tggttcaaaa acattgaata gacctgagtt
     6301 tagagaatta gctccttttt tattctttga taacgaaacc agattcaata catctggtaa
     6361 tccagaactg aaaatcaccg aaatatacaa tgccgatttg agatacgagt tttttccagg
     6421 aaaagggcag ctgttttcag tgtctgcgtt ttataaagac tttaagaatc caatcgaatt
     6481 acaagccttg gccaataact cgaatatata taaaaatgcc aaaagcggaa ccgattatgg
     6541 tgtagagttt gaatttagat ccttattaag tacaatgaca gggatgaagg aacgcaaatt
     6601 ttttgatgat ttgactcttt atgctaatct tgctgtaatt cgttcaaagg tagatatttc
     6661 taatttgttt gaaacaccat atgcaattga agatactcca atgcaaggcc agtcaccctt
     6721 tgttctaaat gcgggaatac tatacacaaa tactgaggct ggctggtcgt tttcagccaa
     6781 tttgaaccga ataggagata gaatttctat tcacgcgaat caaacattag gtaatccggt
     6841 ggcagggttt tgggaaaaag ccagaacttt attagacctg cagttagcta aaacattatt
     6901 taataaaaag cttgaattca aaataaacct tcaaaacgca ttggcccaag atttgatttt
     6961 gtatcaaaac aattatgaat tatcttccac agcaaatcag gtcaaaggat ttaaagcgtt
     7021 taccaacaaa atgattttag gagattctca aaataagaat ggctatgata gtagttatga
     7081 tgatttgaat gcaagaatca aatttggttc cacctattct ttcactttaa cgtactcttt
     7141 ctaagaactt acatacttaa aactctaaga gcctgttttt tataaaacag gctcttaatg
     7201 tttacataac ataatagtgt cttttttata acttttagtt tacccaatat ttacatcacc
     7261 atcttaatat tgctacataa tttaaacaaa caaaaaatga aaaaaaatta cttattttcg
     7321 atgttattac tagtaacatt tactggtttt tctcaaatta ttactactac ttacagaggg
     7381 gcatttgcac cagctccagc tgctatgtgg acagatattt ggacagaata tgaccctcaa
     7441 aacaaagtgt atccaacacc aaccgtaact atttctactg atattactac caatactact
     7501 tggacagctg gaaacactta ttctttagga ggacaaattt atgtcaaaaa taatgctact
     7561 ttaactattg agccaggagt aattattcgt agtactgcac ctggagcagg tttgtttatc
     7621 actaaaggag caaaactaat ggctgaaggg accgctgcca gcccaattgt ttttacgtct
     7681 ggttattctg aagggaatag aagcagaggt gattggggag gtgttatttt gatgggtaaa
     7741 ggatcttaca atatcaatgg cggagtaaat aatattgaag gtatcgcacc aacaaccgac
     7801 acacaatatg gtggaggtgc tacgccagat gataatgaca attcaggttc tttgaaatat
     7861 gtaagaatag agtttgctgg ttatacttat ggagctgcag gaagtaatac agagattaat
     7921 ggtttaacaa tgggagctgt tggtaaagga acaacaattg attatgttca agtttcttat
     7981 gccaatgatg attctttcga atggtttggt ggagctgtaa attgttctca cttagttgct
     8041 tatagaggct tagacgatga ttttgatacc gataatggtt ttagcggaag cgtacaattt
     8101 tgtttggcta tcaaagaacc agctattgca gattcttcag actctaactg ttttgagtcg
     8161 gataacaatt caacaagcac ggctggaact agttttacaa gagccatttt ctctaactgt
     8221 actttgatag gaccttcata tagagccact ttgccaggag gaggagcttt ggccattaga
     8281 catagaagag gagcacactt aagaagaaat acacaacttc aagtttacaa ttctattttt
     8341 atggattttg ttgatggact tgttgtggat ggaactacta ccataacaaa tgctacgaac
     8401 aatactttaa aatttaaaag caatattatt gcaggtacag cagcagcaaa agtagctgta
     8461 aaagtagaat ctccagcaac tactttcgac gtggctactt ggtttacaaa taacaacaac
     8521 acaagtcaaa cggctagcgc tggattattg acattgccat acaacacaac agacggaagt
     8581 ctttacacag gattagatta cagaccagca gtaggttcta tcgctttatc aggtgctgat
     8641 tttactaatt tgggaacaga taaatttgtt tcgaacgctg aattttcttt ggaagtttat
     8701 ccaaatccag tagtaaacag tttcaaacta aattacagtt cttcaagcaa cgaagatgta
     8761 aaagtagcta cttatgacat tacagggaaa ttagtagaat ctattaatgt taaatatgca
     8821 gacattaacg accaagaaat gggtaacaat tataatgcag gtgtttacat cctggttttg
     8881 aaacaaggag acatcaataa atcagttaag gttatcaaaa aataattcaa accgttttat
     8941 aaatctaaaa agtctcgcat ttgcgagact tttttagtta agcaaagaaa ctcaatttca
     9001 aaacttgggc atttgacgac aagttcaaaa taattcctaa atatattgaa gaaacaaaat
     9061 cagcattaaa ccaaagaaat ggttattttt gtctcataaa aattaaaagc cacaccttgg
     9121 gattatataa aaaccttttc aaacaaacag ccatttacgg acttgccaca gtattgccgc
     9181 gaatgctcag ctttctgtta gttcgattat atactggcat tttacccaca accgattatg
     9241 gtgaagtttc cattcttttg tcttggatgg tattcttcaa cgtgattttg tcctacggaa
     9301 tggaaaccgg attttttcgt ttttataatt ccgaaaccga taagcaaaat gtaattgcaa
     9361 cttctaccat ttctatattt tggtccacta ttttcttcct ttttggagcc ttgattttta
     9421 ggaattcctt ggcttcattt gccaatgtcg atgtacaata cattacctat tccatttgga
     9481 ttttggcatt ggatgccttg gtgattattc cgttttcaaa attgcgagcc aatcaacgtc
     9541 cgataatgta tgctgttatt aagataggaa acgtgatagt caacttgatt ctcaacattt
     9601 tctttttagt gtttttgcca caattagcag cagacaatcc tcattctttt ttcgataatt
     9661 tatatgtaga gcattccgaa atagggtata tattcattgc taatttatta gctagtttgc
     9721 taacattggt ggtgctttcg cccaattatt tttcgttgaa ccgaaaattc gacaaggaac
     9781 tttggaaaaa aatgatgaaa tacggactgc ccattcttgt agcaggaatc gcttttgcgg
     9841 tcaacgaaca tttcgataaa attctattgg gatatttact ccccgaaaat attgcaaaat
     9901 ccgaagttgg ggcttattcg gcctgttaca aactcggatt gtttatggtt ttgtttgcca
     9961 ccgctttccg tttaggaatc gaacccttct tttttagtca ttcgtcaagc gaaaatgccc
    10021 cacaaacgta tgccgtgatt accaaatatt ttgtcatttt aggctcatta attctattag
    10081 gagttatcgt ttttgccgat gttttaaaat atctattgct cgacaacaaa tcctattggg
    10141 aagccatgaa agtcgtgccg ttaattattt tagctaactt ttgtctaggc atttacaaca
    10201 acctttctgt ttggtataaa ctgactgata aaacacatat tggtgcttat atttctatag
    10261 ttggagcgat attaacttta gttttaaact atcttttaat tccaaaatac agctattatg
    10321 gttcggcaat tgccaccatt gcggcctacg gaagtatgat gattatctcc tacgttctag
    10381 ggaataaata ttatcccatt ccgtatgata tggaaaaaat tggtggttat ctgggcttgt
    10441 ccatcttgtt ttctgtgatt tatttctacg gatttcgtga aaattatttc gttggaattc
    10501 cgttattact agcgtttatg tattttgtgt atcacaacga gaaagcaaca attttgggta
    10561 tcctaaaacg aaaaaaataa aaattagaat gaaaatcaac atcatcaaca aatcgcaaca
    10621 cgatttacct agttacgaaa ccatagcctc ggcaggaatg gatttgcgtg ctaatctaac
    10681 cgaatcgata accttgaaac ctttggaaag aaccatcgta aaaacaggtc ttttcatcga
    10741 attgcctatt ggttacgaag ctcaagtgcg accaagaagc ggcttggcag ccaaaaaagg
    10801 aattaccgtt ctcaattcac caggaactgt cgatgccgat tatcgtggcg aaattggcgt
    10861 gattttggtg aatttatcca atgaagtttt cgtggtcgaa aatggcgaac gcattgccca
    10921 actaatcatc gccaaacacg aaagagccga atggattgaa gtccaggaat tatccgaaac
    10981 ttcaagagga gaaggcggtt ttggcagtac gggagtgaag taagtgttta gtgttcagtc
    11041 gcagttagca gttttaaaca taaaattaat agttgaaagt atttagttta gtcttaatac
    11101 ttgatactaa aatcttaata caattaaaaa atgaaaataa tagtaccaat ggctggacga
    11161 ggatccagat taagacccca cacattaacc attcctaaac ctttaattcc tattgctgga
    11221 aaaccaatcg tgcatcgatt agtagaagac attgctggag ttttgaatca agatatagaa
    11281 gaaattgcct ttatcattca cgaaagtttt ggcaaaaaag tagaggaaga cttaatcgcc
    11341 attgccgaaa aactgggttc gaaaggaacc atttattacc aaaacgaagc cttgggaaca
    11401 ggtcacgcca ttatgtgtgc caaagattct ttgagcggac cagccgttat tgcctatgct
    11461 gacaccttga ttcgtgccga ttttgattta gatcaaaatg ccgacagcgt gatttgggta
    11521 aaaaaagtag atcaacccga agcttttggt gtggtcaatt tgaacgaaaa aaatcaaatc
    11581 gttgaattgg tcgaaaaacc gaaggaattt gtatctgacc ttgctgttat cgggatttat
    11641 tatttcaaag atgttgcggt tttgaaaaac gaattgcaat tggttttgga caacaatatc
    11701 atcaacggtg gcgaatacca aattaacgac ggaatcaagc aaatgatggc aaaaggtatg
    11761 atttttgtac ccggagaagt ggccgagtgg atggattgcg gcaacaaaga tgttactgtt
    11821 gaaaccaatt ctagaatgtt aggatttttg aatcaagatg gcgaagaatt agtggccaaa
    11881 aacgtaaaac tagacaactc aactatcatt ccgccttgtt atattggtga aaatgtgatt
    11941 ttaatcaatg ctaccgttgg accaaatgta tctttaggga atgcctgtca cgtgagcaac
    12001 agttctataa aaaacagttt ggtacaaacg cattcgcata taaaaaatgc taatttagac
    12061 aacgcaatga taggaagcca cgctagtttt gatggtaatt ttaccagcat tagcattggc
    12121 gattattcgg tgttggagta ataaaaaggt atttattcca tttgaaaaca tataaaaaaa
    12181 tcaacagatt ttaatttgtt gattgttgct atatttgaaa aacagcaatg aaaaaatcgg
    12241 tttccatctt tttgttcttg gttttgcttt gcaattctgc ttcgttattg gcgcaaactg
    12301 aacccgagga aatcaagact gaaaccgacc aattcgagga acatttttat gaatctttga
    12361 agcaaaaagg aatagagaat tacgacaaag ctattacaga attgggatat tgcttgaaat
    12421 tgaaacccaa tgaagccagt gttcattctg agatgggaag aaattattta gcttcaaaac
    12481 aatatgaaaa cgcctattcc tcattcgata aggcaactca aattgatgct aaaaacaaat
    12541 ggttttggat tggaatgtat gatgtttgtt atcaaaccaa gaattacaat cagtgcattg
    12601 ttgttgtcaa taagttaatt gagtttgacc caatttacaa agacgatttg gtttcgctgt
    12661 atatgaacac acaacaattt gacaaagcac tcgttctaat caatgagttg aatgagaaag
    12721 ttggaaaatc ggacatgcgc gacttgtata aagctcaaat tttatcacaa ggaaaatttc
    12781 aaaatgctga aatagataat ttattggctc aaattaagtt gaatccaaag gtggagtcca
    12841 attatgtggc tttaattaag ttatattcag agaagaatga agctcaaaaa gcagtagaaa
    12901 tcactaaaaa attggaagct gaaattccag aatcggagtg gacacaagtg ggtttgttta
    12961 aattttattt agataaaaag gaaggcccaa atgcgattaa ggcaatgaat atagttttgg
    13021 ctagttccaa gatagattct aaaataaaac atcgagtttt gaatgaattt ttgaattttg
    13081 tcaacacaaa tcctcaatat tcgccagatt tagagaaagc aatttcctat tttgataaag
    13141 attcagatgt aaaagttgcc aaagaaattg ggaaatatta tcacgctaaa aagcaatggg
    13201 acaaagccat aacctattat gaattgtctt taaaaaacag ctctaatgaa gatgttgaaa
    13261 ccaatttact tttgcttcag gcctatgtcg aaacgaaaca atttgagatt gtaaaaaaga
    13321 aagcatcttc tttggtcgaa agttatccaa cgcaacccca attttattat ttttcgggtt
    13381 tggcaaacaa ccaattaaaa gaatacaaaa aagccgcaac aatgcttgaa gtaggaatag
    13441 attttgtggt agaagatgta attttggaag ctaatttcaa tttacaatta ggcaaggcct
    13501 acaacggatt aggagatttt aagaaaaaag aaatgtattt ttctaaagcc aatcaattat
    13561 taagagccaa aaaataagat agcgaatgaa taaatttttc aaaaattgta aaaccttttt
    13621 tttggttagc ttcatcagct cattaacatt ggtgtcgtgt aaatcgaaaa cagcagctgt
    13681 tgatgtgtcg aaaactgaaa acaaaaaaag catcaataaa attatagaaa actattacaa
    13741 caataaaact gatttttcga cattatatat aaaagcgagt gtacaatatg ctagtgagaa
    13801 acaaaatcag aatgtaaccg ccgaaattaa actcaaaaaa gacgagcaaa ttctaattag
    13861 catacggttt ttgggaatta caatggcaaa agcttcgatt acgccaacca aagtgagcta
    13921 ttacgaaaaa gtgaaaagca cgtattatga aggtgatttc agcgctttga gtcaattgtt
    13981 gggcactgat ttagacttta gtaaggttca aaatatgttg ttgggaagag caatcgataa
    14041 tttgaaagaa ggaaaatata cagaatcatt agaaaatcaa gtctatcgat tagacgaaac
    14101 ggccgatgat aaccttaaga aatcttttta tattgacgcc aatagtttct tgatgaacaa
    14161 acaagaaatc acgcaaacca aagaagaaag aatgattcag gttgtgtatt ccaatccaaa
    14221 agaattcaaa gaaatgactt tgccgttgaa cataaatatt gatacttatc aaaagaaagg
    14281 aaaagccgaa atcaatttag aatacaacac cgtaagtttc aatgaagaac tttcttttcc
    14341 ttatagcgtt ccaaatgatt ataaaagaat tataattaag taaatttgca caaacatatt
    14401 tgtactatgc caaaatttct cctaagcctg
//
LOCUS       DAOSNN010000035        14192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_4314_length_14192_cov_6.242767, whole genome shotgun sequence.
ACCESSION   DAOSNN010000035 DAOSNN010000000
VERSION     DAOSNN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4314_length_14192_cov_6.242767"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLQ70_03215"
     CDS             complement(<1..780)
                     /locus_tag="PLQ70_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HQE33654.1"
                     /translation="MILLIIIGVILLIVSFAVKSNASNPFFKFSNTLKLVGFVVIALG
                     IFSSMFKQIDAGKVGVKSLYGSVQPDILESGLHLINPLLDVTDFDIQTQNYTMSAVHG
                     EGAQEGDDAIRVLSNDGLEVVIDLTVLYRITPTDAPKIFKQIGVNYSDKIVRPITRTR
                     IRDNAVYYDAVALYSTKRNEFQQRIRKSIEDDFKARGLILEQLLIRNINLPESVKATI
                     ESKINAEQEAQKMTFVLQKEKQEAERKRVEAQGIADYQRIIS"
     gene            complement(867..2240)
                     /locus_tag="PLQ70_03220"
     CDS             complement(867..2240)
                     /locus_tag="PLQ70_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQE33655.1"
                     /translation="MNISKYTKEFSYSLKLAFPVILGMLGHTLIGIVDNFMVGKLGST
                     ELAAVSLGNSFIFIGMSLGIGFSTAITPLIAEADAEKDDKKIRTTFHHGLLLCTFLGI
                     VIFVLIILAKPIMQLMNQPEAVVELAAPYINWVAFSLIPVIIFQGYKQFADGLSQTKY
                     SMYAIYLANVVHIFFNYVLIYGVFGFPKLGILGAALGTVISRIMMVVFMHFILRKNSA
                     FKHYFKNFTFKEIKKSILKKIINLGLPSAMQMLFEVALFTAAIWLSGSLGKNSQAANQ
                     IALTLASSTFMVAMGFSVAAMIRVSNAKGAKDYKQLITVARSIFLLAIMVETFFGLVF
                     VIFHNFLPHLFLNMSDVTQELDNAEIIFIASQLLLVAAVFQISDGIQVVVLGALRGLQ
                     DVKIPMYITFVAYWIVGFPISYFLGTHTNLAATGIWIGLLAGLTAAALFLYIRFARLT
                     KRLALEN"
     gene            2366..3196
                     /locus_tag="PLQ70_03225"
     CDS             2366..3196
                     /locus_tag="PLQ70_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33656.1"
                     /translation="MEKFTLEALFKIIGLGSASGLVYKDNSLFIISDNSTFLYQYHIQ
                     EKQLSKIQLLEKGEENIPKKDKYDFESIVLKGNKLHLLGSGSTSKREKRITYNLETKE
                     IIEKDLSKLYKNLKQIAPISDEELNIEGVVFDNEKWLLFQRGNGVNSKNGIAILNGKL
                     NKNSKIEFISIPLPKIKHIETSFTDAIFVDEKIYFLATAEDTTSTYDDGEILGSLIGR
                     MDSKTFEIEFTQKIPDTQKFEGLTVYQISETQIEFLLCEDNDSDVLETNIYKISVPKF
                     "
     gene            3305..3805
                     /locus_tag="PLQ70_03230"
     CDS             3305..3805
                     /locus_tag="PLQ70_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQE33657.1"
                     /translation="MHRDAQRQVYELMAPKLYRTCKRYLKKEEEIEEALADAFYTIFT
                     KLDQLKELGAFEAWSRKITVNHCLATIKKKTNFNLYLEDVKTISQPFTDEVTELEEED
                     LLKLLNHIPEGCKTIFNLFVIEGYGHKEIAAMLNISEGTSKSQLNASKTKLRELVNNL
                     YYQKAK"
     gene            3808..4743
                     /locus_tag="PLQ70_03235"
     CDS             3808..4743
                     /locus_tag="PLQ70_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33658.1"
                     /translation="MDNQDKIFDKIKNAAHKAEEKDFPGMERIWQRVDAKLEQKELQS
                     KNKNWKKIAIAASVILLVSLGYQFLKPTEITSIDNEVVVKKVPSKNILKKAIVSSQID
                     TAKVINVLNETKTTLVEKENQSPVVASSAMASAEKQSEMADGIIASDYTKSEKETILR
                     NKTNTFRNQVFDAIVVRSAYSELEKKKQKDSIAPQKAAETNGPLVVVNGNLAQTKFKS
                     VQEAKKEMPKKLDNEEIESLIVLKEPLYIINGVEYTEESLFGTHPTSPYAPLNKQKIE
                     TLTILQDDEAIEKYGEKGSKGVVIITTKNRVPIQK"
     gene            4784..6718
                     /locus_tag="PLQ70_03240"
     CDS             4784..6718
                     /locus_tag="PLQ70_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HQE33659.1"
                     /translation="MKTTKRISLALLMLFSVLSFGQKTTITGIVTEDTGAPLPGVSIY
                     IKGTKNATSTNIDGKYSILAEKGDKLVFSYIGFETKYATVGKSTIINITLKSSSNQLN
                     EVVVVGYGTSENADYSGSVAMISANKLNKQKSANVNDALQGRVAGVQISSKSEEKKRI
                     KNRKIKDTIITAKPIKITDEDYGTFIENPFESPKFAPLSTFSIDVDNASYTNVRRFIN
                     NGQTVPKDAVRVEEMVNFFKYKYPQPQDEHPFSINTEYSECPWNSNHKVLKIGLQGKN
                     IPTESLPASNLVFLIDVSGSMSSDNKLPLLKQSLKILVNELRKKDKVAIVVYAGAAGM
                     VLPPTTGDEKQTIIDALEKLNAGGSTAGGAGIELAYKLAQENFVKGGNNRVILATDGD
                     FNVGASSNTDMQTLIEEKRKSGVFLTCLGYGMGNYKDSKMETLADKGNGNYAYIDNIQ
                     EANRFLGKEFKGSMFAIAKDVKIQIEFNPKQVQAYRLIGYENRKLRPEDFTNDAIDAG
                     ELGSNHTVTALYEVIPTRVKSEFLNNQPELKYTKTTASEDNYSDELATIKFRYKKPDG
                     DKSIEMVKVIENQSIPLKNASDDFKFSTAVAWFGLKLRDSNLVPNKSSEEIKKLSKEG
                     LSNDEDGYKAEFIRLVETVK"
     gene            complement(6773..8101)
                     /gene="mtaB"
                     /locus_tag="PLQ70_03245"
     CDS             complement(6773..8101)
                     /gene="mtaB"
                     /locus_tag="PLQ70_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HQE33660.1"
                     /translation="MENKKKVAFYTLGCKLNFSETSTIARSFQDEGFERVDFEEVADM
                     YVINTCSVTENADKQFKQVVRKAMKLNNKAFVAAIGCYAQLKPEELANVDGVDLVLGA
                     TEKFKITDYINDLSKNDFGEVHSCEIEEADFYVGSYSIGDRTRAFLKVQDGCDYKCTY
                     CTIPLARGISRSDELQNVLKNAKEISEQGIKEIVLTGVNIGDYGKGEFGNKKHEHTFL
                     ELVQALDQVEGIERLRISSIEPNLLKNETIEFVSKSRTFVPHFHIPLQSGSNEILKLM
                     KRRYLREVYTERVNKIREVMPDACIGVDVIVGFPGETDEHFLETYHFLNDLDISYLHV
                     FTYSERDNTEAAEMEGVVPNNVRAKRSKMLRGLSVKKRRAFYESQIGTNRTVLFEAEN
                     KEGYIHGFTENYVRVKTPWNPELANTLHEINLTKIDEDGSVRLEFLNVEV"
     gene            complement(8262..9464)
                     /locus_tag="PLQ70_03250"
     CDS             complement(8262..9464)
                     /locus_tag="PLQ70_03250"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQE33661.1"
                     /translation="MVQRKKYIEIVKHLSKKEFTILIGARQIGKSTLLKQLFEDLNQK
                     GETVYFLNLDRKEILDELDESPENLFKICPLQTDKKIIVFIDEIQYLKDATTFVKLLF
                     DEYSDRLKIVATGSSAFYIDKQFNDSLVGRKKIFQMGTLDFEEFLIFKGRNDLVEEVL
                     KLKNKKKQKSIQENVLWAFMDEFVNYGGYPAVVLENDIAQKIELLKDIRDSFIKRDIL
                     ESGITDETKFYRMLMLLASQSGNLLNVNEISNTLRMTNATVENYLYVLQKCFHITLVK
                     PFYNNLRKELTKMPKVYINDLGLRNVLINYFSPLEQRADKGMVLENLCFRLLFERFDQ
                     DQVKYWRTADGNEVDFVVETSYLKGFAVEVKFGEQEVKSSKYNKFIQNYPDFPLEFWW
                     WKKMELLF"
     gene            complement(9627..10802)
                     /locus_tag="PLQ70_03255"
     CDS             complement(9627..10802)
                     /locus_tag="PLQ70_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HQE33662.1"
                     /translation="MNYILFDGPARNALLPFTFTRPVADILVGIMTIRQKWEMRLGST
                     TTTITEEYLSEKFPMVEMEENVMINASFLPNSDLVEMVTNLEINQAIFQGDDVIAFHT
                     HENQQEVDFETYEIIEFQGECIRIEHTCDIFSKNAAAIKEDFELLTEERKSQPIPRSV
                     NTIAPENIFIEEGAKLEFVTLNATNGPIYIGKNTEIMEGSVIRGPFALCEGAIVKMMA
                     KIYGATTIGPYSKIGGEVNNAVLFAHSNKGHDGFLGNSVLGEWCNIGADSNTSNLKNN
                     YEEVKLWNYETESFEKTGLQFCGLMMGDHSKCGINTMFNTGTVVGVSANIFGAGFPRN
                     FVPSFSWGGAAGITVYMTRKAFETARLVMSRRNIEFDEKEAAILQHVFDETKIWRKE"
     gene            complement(10945..11196)
                     /locus_tag="PLQ70_03260"
     CDS             complement(10945..11196)
                     /locus_tag="PLQ70_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HQE33663.1"
                     /translation="MKKGIHPENYRLVAFKDMSNEDVFITKSTAETRETITVDGVEYP
                     VVKMEISRTSHPFYTGKSKLIDTAGRIDKFKTKYAKHGK"
     gene            11443..11970
                     /locus_tag="PLQ70_03265"
     CDS             11443..11970
                     /locus_tag="PLQ70_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HQE33664.1"
                     /translation="MNEIIKKNGISFGIITGVVSALLTTAIYSIDLNLFTKWWLGILM
                     IIVYIIIGIVLLSKTKKELKGIFSFKEAFTTYFISAVIGILISVAFNILLFNVIDPSV
                     KDTLNEIVIKYTAETMQKFGAPSAAINEAIKKMQENNPYSTIELLKGCVFSIVGSSLF
                     GLLLALIFKSKPSQE"
     gene            11978..12931
                     /locus_tag="PLQ70_03270"
     CDS             11978..12931
                     /locus_tag="PLQ70_03270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQE33665.1"
                     /translation="MNLSILIPLLNEDESIKELYTWIISVMKSNNYSYEILFIDDGST
                     DKSWTIIENLAAENPNVKGIRFMKNFGKSQALHAGFAKAQGDVIITMDADLQDSPEEI
                     PGLYEMITAQNFDLVSGWKKKRYDSVVAKNLPSKLFNWAARKTSGVQLNDFNCGLKAY
                     KNVVVKNVEVSGEMHRYIPVLAKNAGFNKIGEKVVQHQARKYGETKFGMDRFINGFLD
                     LITIWFLSRFGKRPMHLFGALGSMMFMIGFVAAGYIGISKLYHMYSGMKYTLVTNNPW
                     FFIALTTMVLGTQLFLAGFLGEIILRTKNNEERYKVWKEIN"
     gene            13003..>14192
                     /locus_tag="PLQ70_03275"
     CDS             13003..>14192
                     /locus_tag="PLQ70_03275"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HQE33666.1"
                     /translation="MEIKQNILDAVNEWLTPAFDAQTQEAVIEMMTSSPKDLEESFYK
                     NLEFGTGGMRGIMGVGNNRINKYTLGKSTQGLSNYLKIAFPNRPLKAVIAFDCRHNSK
                     TLAKVVADVFSANGIQVYLFSDLRPTPELSFALKYLGCQCGIVLTASHNPPEYNGYKV
                     YWEDGGQIVPPQDGAIIDTIEKLNYNQIKFDANEDLIQYIDAEIDQAFIKSTIENASF
                     GTSQAAKDNLNIVFTSLHGTSITLVPDTFAQAGYKNVNIVEEQRVPNGDFPTVKSPNP
                     EEPEALTLALALADKTNADIVVGTDPDCDRLGVAVRNNEGKMILLNGNQTMILMTAFL
                     LEEWKKAGKINGKQFVGSTIVSTPMIMELASAYEVGFKVGLTGFKWIAKMIKDFPELE
                     FIGGG"
BASE COUNT         4892 a         2657 c         2329 g         4314 t
ORIGIN      
        1 agaaataatc ctttgataat cagcgatacc ttgtgcctca actctttttc tttcggcttc
       61 ttgtttttct ttttgtagca cgaaagtcat tttttgagcc tcttgctctg cattaatctt
      121 gctttcgata gttgctttta cagattcggg aagatttata tttcgaatca atagttgttc
      181 taaaatcaaa cctctagctt taaaatcgtc ttctatactt tttctaattc tttgttgaaa
      241 ttcatttctt ttggtcgaat acaaagccac ggcatcataa taaacagcat tatctcgaat
      301 acgagttcta gtaattggac gcacaatttt atcgctgtaa tttactccaa tttgcttgaa
      361 aatctttgga gcatcagtag gagttattcg atacaaaacg gtcaaatcaa tcaccacttc
      421 gagtccatca ttcgacagaa ctcgaatagc atcatcgcct tcctgagcgc cttcgccgtg
      481 aacggctgac atagtgtagt tctgggtttg aatgtcaaaa tcggttacat ccaaaagtgg
      541 gtttatcaaa tgcaaaccac tttctaaaat atcgggttgt acactaccat aaagtgattt
      601 tacacctact tttcccgcat caatttgttt gaacatcgac gagaaaattc ccaaagcaat
      661 tacaacaaat ccaactaatt taagcgtatt cgaaaacttg aaaaacggat tgctggcatt
      721 actttttaca gcaaaactca caatcaataa aataacccca ataataatta atagtatcat
      781 tttttttcaa aattaatttg ttcgatgatt atacgataaa tcagattata agttacaatg
      841 ttttaagaaa aaacctaaaa attttattag ttttccaatg ctaatctttt ggtcaatctc
      901 gcaaagcgaa tgtacaaaaa taaagctgcc gctgttaatc ctgccaaaag tccaatccag
      961 attccagttg cggccaaatt ggtgtgtgtt cctaaaaaat acgaaatcgg gaaacccaca
     1021 atccaatatg ccacaaaggt aatgtacatc ggtattttta cgtcttgcaa ccctcttaaa
     1081 gcccccaaaa ctaccacttg gattccgtca gaaatttgaa aaacagctgc caccaaaagc
     1141 aattgggatg ctatgaatat tatttcggca ttatccaatt cttgtgtgac atcggacata
     1201 ttcaagaaca aatggggcaa gaaattatga aaaattacga aaaccaaccc gaagaaagtt
     1261 tcgaccataa tcgccaacaa gaaaattgag cgagccacgg taattaattg cttgtaatct
     1321 tttgcccctt tggcattgct cactcgaatc atcgctgcca cgctaaaccc cattgccacc
     1381 ataaaagtgg acgaagccaa agtcaacgca atttgattgg ctgcctgact gttttttcca
     1441 agcgaacccg aaagccaaat cgctgcagta aacaaagcta cttcaaacaa catttgcata
     1501 gccgaaggta aacccaaatt aatgattttc tttaaaattg atttcttaat ttctttgaaa
     1561 gtaaaattct tgaaataatg tttgaatgct gaatttttcc tcaaaataaa gtgcataaaa
     1621 accaccatca taattctaga aattacagtt cccaatgctg cgcccaaaat acccaattta
     1681 ggaaaaccaa aaaccccata aatcaacaca taattgaaga aaatatgtac cacattggcc
     1741 agataaatgg catacatcga atatttagtt tgcgacaaac catcggcaaa ttgcttataa
     1801 ccttggaaaa taattacggg aattaaggaa aaagccaccc aattaatata aggcgcggcc
     1861 aattcgacaa ccgcttctgg ttggttcatt aattgcatta tgggctttgc taaaataatc
     1921 aaaacaaata tgacgattcc caaaaaagtg cacaataaca ggccgtgatg aaatgtagtt
     1981 ctgatttttt tgtcgtcttt ctcggcatca gcttccgcaa tcaagggcgt gatggcggta
     2041 gaaaaaccaa ttcccaacga cattccaata aagataaaac tattgcccaa tgaaacggct
     2101 gccaattcgg ttgaacccaa ctttccaacc ataaaattat ctacaatacc aattaatgta
     2161 tgccctaaca ttccaagaat aactggaaaa gctagtttca aactataaga gaactctttt
     2221 gtatatttgg aaatattcac attaatttat tttggttggc aaagatatag caaaaacaca
     2281 acacaaattc gattgatttt tactaatttc gaaaagttta caattgtcct ccattagaaa
     2341 acccaaactg tctcttcatt attatatgga aaaatttaca ctcgaagcct tatttaaaat
     2401 catcggtctt ggctcagctt ctgggctagt ttacaaagac aattccttgt ttatcatttc
     2461 tgataacagt acttttctat atcaatatca cattcaggaa aagcaacttt ctaaaataca
     2521 attattagaa aaaggcgaag aaaacattcc aaaaaaagac aaatacgatt ttgaatccat
     2581 tgttttaaaa ggaaataaac ttcatctttt gggttccggt tccacttcca aaagagaaaa
     2641 aagaataacc tataatcttg aaaccaaaga aataattgaa aaagacttat caaaattata
     2701 taaaaatcta aaacaaatcg ctcctatttc tgatgaagaa ctcaatattg aaggagtcgt
     2761 tttcgataac gaaaaatggc ttttattcca aagaggcaat ggtgtcaatt caaaaaacgg
     2821 aatcgcaatc ctaaatggca aactcaataa aaatagtaag attgagttta tttcaatacc
     2881 attaccaaaa atcaaacaca tagaaacttc ttttacagat gccatttttg tcgatgaaaa
     2941 aatttacttt ttggcaacag ccgaagatac tacttccact tacgatgatg gcgaaatatt
     3001 gggaagccta attggacgaa tggacagcaa aacattcgaa atagagttta cccagaaaat
     3061 acccgatacc caaaaatttg aaggattaac agtttatcaa ataagtgaaa cccaaattga
     3121 gttcctactt tgtgaagaca atgattcgga tgttttggaa accaatattt ataaaatctc
     3181 ggttccaaaa ttttaaataa caaaaatatt tcaaccgctt cccaaccttt tacccttaat
     3241 catcctcttt agtagaaaga caacaattgt gataaacgaa aaactaattt ctgattgcaa
     3301 aaaaatgcac cgcgatgccc agcgtcaggt gtatgagctg atggctccca aactgtatcg
     3361 tacttgcaag cgctacctaa agaaagaaga agaaatagaa gaagccttgg ccgacgcttt
     3421 ctatacaatt ttcacaaaac tcgatcagct caaagagttg ggagctttcg aagcttggag
     3481 cagaaaaatt actgtcaatc attgcttggc aaccatcaaa aagaaaacaa atttcaattt
     3541 gtacctcgaa gatgtcaaaa ctatttctca accttttacc gatgaagtca ccgaactcga
     3601 agaagaagat ttgctgaaat tgctcaacca cattcccgaa ggttgcaaaa ctatatttaa
     3661 tcttttcgtt atcgaaggtt atggtcacaa agaaatcgca gcaatgctca acatttccga
     3721 aggcacatcc aaatcacaat tgaatgcttc gaaaactaaa ttaagggaat tagtaaacaa
     3781 tttgtattac caaaaagcga aatagtcatg gacaatcaag ataaaatatt cgacaaaata
     3841 aagaacgctg ctcacaaagc tgaagaaaaa gactttcccg gaatggaaag gatttggcaa
     3901 cgagtggatg ccaaattgga acaaaaagaa ctacaatcca aaaacaaaaa ttggaaaaaa
     3961 attgccattg cagcatcggt tatcctcttg gtttccttgg gatatcaatt cttaaaacca
     4021 actgaaatca cttcaataga caatgaagtt gtagtaaaaa aagtgccttc caaaaacatt
     4081 cttaaaaaag cgattgttag ttcgcaaatt gacaccgcca aagtaataaa tgtccttaat
     4141 gaaacaaaaa caactcttgt tgaaaaggaa aatcaaagcc cagttgttgc atcaagcgca
     4201 atggcttcag cagaaaaaca gagtgaaatg gcagatggaa ttatagcatc ggattataca
     4261 aaaagtgaaa aggaaactat tttaaggaat aaaaccaata ctttccgaaa ccaagttttt
     4321 gatgcgatcg tggtgcgatc ggcttattcc gaattagaaa aaaagaagca aaaagatagt
     4381 attgcacccc agaaagcagc cgaaaccaat gggcctttgg ttgttgtgaa tggaaattta
     4441 gcacaaacaa aattcaaatc agttcaagaa gcaaaaaaag aaatgcccaa aaaactagat
     4501 aacgaagaaa ttgaatcgct cattgttctc aaagaacctt tgtatatcat caatggtgtc
     4561 gaatatactg aagaatcgct tttcggaaca catccaacaa gtccttatgc tcctttgaat
     4621 aaacaaaaaa ttgaaactct aaccatttta caagacgacg aagccataga aaaatatgga
     4681 gaaaaaggta gcaaaggagt cgtaattatt accaccaaaa acagagtacc catccaaaaa
     4741 taattcgaaa aaaatcattt ctaacatact aaaaacaact attatgaaaa ctacaaaacg
     4801 tatttcatta gccctactta tgcttttttc tgtcctaagt tttggccaaa aaacgactat
     4861 aacaggtatc gtaaccgaag acacgggcgc gccacttcct ggtgtatcta tttacattaa
     4921 aggaactaaa aatgcaacta gcacgaacat cgatgggaaa tacagtattc tcgcagaaaa
     4981 gggtgataaa ctcgttttct cttacattgg gtttgagaca aaatatgcta cagttggaaa
     5041 atcaaccatt ataaacatta ctctaaaaag cagttcgaat caattgaatg aggtggttgt
     5101 tgtgggttat ggaacatccg aaaatgctga ttattcaggc tcggttgcaa tgatttctgc
     5161 taataaatta aataaacaaa aatcagctaa tgttaatgat gcgcttcaag gaagagtagc
     5221 tggagttcaa ataagttcta aatcagaaga aaaaaaacga ataaaaaata gaaaaattaa
     5281 agacactata attactgcaa aacctataaa aattactgac gaagattacg gaacattcat
     5341 tgaaaaccct ttcgaaagtc caaaattcgc tccactttct actttttcta ttgatgtgga
     5401 caatgcttct tatacgaatg taagacgttt tatcaacaac gggcaaaccg ttcctaaaga
     5461 tgccgtaaga gtcgaagaaa tggtgaattt tttcaaatac aaatacccgc aaccacaaga
     5521 cgagcatccg ttttcgatta ataccgaata cagcgaatgt ccttggaatt cgaaccacaa
     5581 agtattgaaa attggcttgc aaggcaaaaa tattccaacc gaaagtttac ccgcttccaa
     5641 cttggttttc ttgattgatg tttcgggttc gatgagttct gataataaat tgcctttact
     5701 gaaacaatcg ctaaaaattt tggtcaacga actccgaaaa aaagacaaag tggccattgt
     5761 ggtttatgcg ggagcagcag gaatggtgtt gcctccaacc actggagacg aaaaacaaac
     5821 tatcatcgat gcgttagaaa aactcaacgc tggcggaagc actgctggcg gtgcaggaat
     5881 tgaattggct tataaattgg ctcaagaaaa tttcgtcaaa ggtggaaata accgagtaat
     5941 attggctact gacggcgatt ttaatgtggg agcatcttcg aatactgata tgcaaacctt
     6001 gattgaagag aaaagaaaat caggtgtttt cttgacttgc ctaggttatg gaatgggaaa
     6061 ttacaaagat agcaaaatgg aaactttagc cgacaaaggc aatggaaatt atgcttacat
     6121 cgacaacatt caagaagcca atcgtttctt gggcaaagaa ttcaaaggtt ccatgtttgc
     6181 cattgccaaa gacgtgaaaa tccaaattga attcaatcct aaacaagtgc aagcctaccg
     6241 attaattggt tacgaaaacc gaaaattacg ccccgaagat tttacaaacg acgctattga
     6301 tgcgggagaa ttaggaagca atcatacggt taccgctttg tatgaagtga ttccaaccag
     6361 agtaaaaagc gaattcctga acaaccaacc cgaattgaaa tacaccaaaa caactgcatc
     6421 cgaagataat tattccgatg aattggctac catcaaattc cgttacaaaa aacccgatgg
     6481 agataaaagt atagaaatgg taaaagtgat tgaaaaccaa tccattccat tgaaaaatgc
     6541 ttctgatgat ttcaaattta gcactgctgt cgcttggttt ggattgaaat taagagattc
     6601 caatctggta ccaaataaat cttcagaaga aataaaaaag ctatccaaag aaggattgtc
     6661 caatgatgag gatggttata aagcagaatt tatccgcttg gttgaaactg tgaaataaga
     6721 gattctataa aaaaaacacc cgacaggttt caaaaatctg tcgggtgttt ttttaaactt
     6781 caacattcaa gaattccaat ctcacacttc catcttcatc tattttagtc aaattgattt
     6841 cgtgtaaagt gttggccaat tctggattcc aaggcgtttt tactctcacg taattttcgg
     6901 taaatccgtg aatgtaacct tctttatttt cagcttcaaa caaaacggtt ctgttggttc
     6961 caatttgact ttcataaaaa gctctacgtt ttttaactga taatccacgc aacattttgc
     7021 ttcttttggc acgaacgtta ttaggaacaa caccttccat ttctgcagct tctgtattat
     7081 ctcgctctga ataagtaaaa acgtgtaaat acgaaatatc taaatcgttc aaaaagtgat
     7141 acgtttcaag aaaatgttcg tcagtttcgc ccggaaaacc aacaatcacg tccacaccaa
     7201 tgcaagcatc tggcatcact tctcgaattt tatttactcg ttccgtataa acctcccgaa
     7261 ggtaacggcg tttcatcaac ttcagaattt cattgcttcc agattgcaac ggaatgtgaa
     7321 aatgcggcac gaaagttcgg cttttcgaaa caaattctat tgtttcgttt ttcaataaat
     7381 ttggttcgat ggaggatatt cgcaatcttt cgattccttc tacttggtct aaagcctgaa
     7441 ctaattccaa gaaagtatgc tcgtgttttt tatttccaaa ttctcctttg ccgtaatcac
     7501 caatgtttac accggtcaaa acgatttctt tgattccttg ttccgaaatt tctttggcat
     7561 ttttgagtac attttgcaat tcgtcacttc gggaaattcc tcttgccaat ggaatcgtgc
     7621 aataagtaca tttgtaatcg caaccgtcct gcactttcaa gaaagcacgg gttctatcgc
     7681 caatagaata actgcccacg taaaaatcgg cttcctcgat ttcgcaagaa tgcacttctc
     7741 caaaatcatt tttggataaa tcgttgatgt aatcagtaat tttaaacttt tctgtagcac
     7801 ccaaaaccaa atccactcca tcgacattgg ctaattcttc gggttttaat tgggcataac
     7861 aaccaatagc ggcaacaaaa gccttgttgt tgagtttcat cgcttttcga acgacctgtt
     7921 tgaattgttt atcggcattt tccgttaccg aacaggtgtt gatgacatac atgtcggcca
     7981 cttcttcaaa atcaacgcgt tcaaaacctt cgtcctgaaa acttctggca atggttgaag
     8041 tttccgaaaa attcaattta cagcctaaag tataaaaggc tacttttttc ttgttttcca
     8101 taaaatagct caattaatga aatcgttgtc aaaaaattga ggttgcaaat ttacaaacaa
     8161 tatttctata attgacaaaa taaaaatccc gaaaataaaa ccgctgattc gcaaatgaaa
     8221 taattaattt gcgaatttgc ggcaatcaag ataacgaatc tctaaaacag caactccatc
     8281 tttttccacc accaaaactc caaaggaaaa tcaggataat tttgtataaa tttattgtat
     8341 ttgcttgact ttacttcctg ctcgccgaac tttacttcta ctgcaaaacc ttttaaataa
     8401 gaggtttcta ccacaaaatc gacttcattt ccatcggcgg tgcgccaata ttttacttga
     8461 tcttgatcaa acctttcaaa aagtaatcta aagcacaaat tttccaaaac cattccttta
     8521 tcagctcttt gttctaaagg cgaaaaataa ttaatcagca catttcgcaa gcccaaatcg
     8581 ttgatatata ctttgggcat cttggtcaat tccttgcgta aattgttgta aaatggtttt
     8641 accagcgtaa tatgaaaaca tttttgcaac acatacaaat aattttcgac agtggcattg
     8701 gtcattcgca acgtattcga aatttcgttt acattcaaca aattccctga ttgacttgcc
     8761 aaaagcatca acattctgta aaacttggtt tcatccgtaa tacccgattc tagaatatct
     8821 cgcttgataa aggaatctcg aatatctttc aataattcta ttttttgagc aatatcattt
     8881 tctaaaacca cggcaggata accaccataa ttcacaaatt catccataaa agcccaaagt
     8941 acattctctt gaatggattt ttgttttttt ttgtttttga gtttcaaaac ttcttctacc
     9001 aaatcatttc ttcccttaaa gatcaaaaac tcctcaaaat caagtgttcc catctgaaaa
     9061 attttctttc tccctacaag cgaatcatta aattgcttgt cgatataaaa agcagaactt
     9121 cctgtggcaa caatcttcaa tcgatcggaa tattcgtcga aaagtaattt tacaaaagta
     9181 gtcgcatctt ttaggtattg gatttcgtca ataaaaacaa tgattttttt atcggtttgc
     9241 aacggacaaa ttttaaacaa attttcagga ctttcgtcca attcgtctaa aatttctttt
     9301 cgatctaaat tcaagaaata aaccgtttct cctttttggt tcaaatcttc gaacaattgc
     9361 ttcaatagcg tggactttcc tatttgccta gcgccaataa gaatagtaaa ctcttttttc
     9421 gaaagatgtt ttacaatttc gatatatttt ttccgttgta ccatactaca aatgtaaaca
     9481 taaaacacaa aatatcggat aattttaaaa aatattttaa aattatccga tattttttac
     9541 tatacacaat aaaaaatatc cgataattta cattgtgtag tatgtaaatt atcggatatt
     9601 tttagtttta aactcttaaa agaaacttat tctttcctcc aaattttggt ttcgtcaaaa
     9661 acgtgttgca agatggctgc ttccttttcg tcaaattcaa tatttctgcg actcattact
     9721 agtcgagccg tttcaaaggc tttgcgagtc atataaaccg ttataccagc tgctcctccc
     9781 caagaaaaac tcggcacaaa gtttcgtgga aatccagcac caaaaatatt agcactcact
     9841 ccaaccacag ttcctgtatt gaacatcgtg ttgataccgc atttactatg atctcccatc
     9901 attaatccac aaaattgaag tcctgttttt tcaaaacttt cagtttcata gttccatagt
     9961 tttacttctt cataattatt ttttaagttg gaagtatttg aatcggcacc aatattacac
    10021 cattcaccaa gcactgaatt gcctaaaaat ccatcgtgac ccttattaga atgggcaaat
    10081 aaaacagcat tattcacttc accaccaatt ttcgaataag gtccaatggt agttgctcca
    10141 taaattttcg ccatcatctt aacgatagca ccttcacaca gcgcaaacgg ccctcgaata
    10201 acagaacctt ccattatttc ggtatttttc ccaatataaa ttggtccatt agtagcattc
    10261 agtgtaacga attccaattt tgctccttct tctatgaata tattttcagg tgcaatggta
    10321 ttgacacttc taggaatagg ttgtgatttt ctttcttcgg tcaaaagctc aaaatcttct
    10381 ttaatggcag ctgcattttt agaaaaaata tcacaagtat gttcaattcg gatacattcg
    10441 ccttgaaact ctataatttc ataagtttca aaatcaactt cttgctgatt ttcgtgcgta
    10501 tgaaaggcaa ttacatcatc gccttgaaaa atagcttgat tgatttcaag attagttacc
    10561 atttccacca aatctgaatt gggcagaaac gatgcgttta tcatcacatt ctcctccatt
    10621 tccaccatcg gaaatttttc ggataaatat tcctcagtaa tagtagtcgt ggttgaacct
    10681 aaccgcattt cccatttttg acgaatcgtc ataataccca caagaatatc agcaacaggt
    10741 cgcgtgaaag taaaaggtaa taatgcattt ctggctggtc cgtcgaaaag gatataattc
    10801 attttgtttt tttttatttt gttagagaca ttgcactgca acgtctatac tttagaaatt
    10861 caaagttaca aagttttgga tgcaataaaa ataaaaaaag tcccgataaa atcgagactt
    10921 tctatattat taaactgata attattattt accgtgttta gcgtatttag ttttgaattt
    10981 atcaatacga cctgcagtat cgataagttt agatttacct gtgtaaaaag ggtgagaagt
    11041 tctagaaatc tccattttta caactggata ttcaacacca tcaactgtga tagtttctct
    11101 agtttctgca gtagatttag tgataaaaac gtcttcattg gacatatctt tgaaggcaac
    11161 taatctgtaa ttttctgggt gaattccttt tttcatcttt atttttttta tttttttgag
    11221 cgttttaatt ttgaagcttt ctcgactttc gaaaaaggtg taaatgcaac tactcttttt
    11281 gtttaaaatc cttttctcat tttgagtttg caaatttaca cttatttttt aataaacaaa
    11341 cctttagaat acttttttta tataattcta aaaaccgatt tttactcgtc aatatattgg
    11401 gaattactat atttgtagat taataaataa atactttcaa cgatgaatga aattataaag
    11461 aaaaacggaa tttcctttgg gattattact ggtgttgtga gcgcattact aaccacagcc
    11521 atatattcaa ttgatttaaa ccttttcact aaatggtggc taggcatttt aatgattata
    11581 gtttacatta ttattggaat agttctatta tcaaaaacta aaaaagagct aaaaggcatc
    11641 ttctccttta aagaagcgtt taccacgtat tttatttctg ctgtaattgg aatattaatt
    11701 tcagttgcat tcaatatttt attattcaat gttatcgacc cttcagtaaa ggacacatta
    11761 aatgaaattg tcataaaata tacagctgaa acaatgcaaa aatttggcgc accatctgct
    11821 gcaattaatg aagcaataaa gaaaatgcaa gaaaacaatc cttattctac aattgaacta
    11881 ttaaaaggtt gtgtttttag catagttggg agttctcttt ttggattact attggcttta
    11941 atctttaaaa gcaaaccttc tcaagaataa aaaataaatg aatttatcca tacttattcc
    12001 gcttctcaac gaagacgaat cgatcaaaga attatatact tggattatct ctgtaatgaa
    12061 atccaacaat tattcgtacg aaatcctttt tattgatgat ggaagcaccg acaaatcttg
    12121 gactatcatc gaaaatttgg ctgccgaaaa tccgaatgta aaaggcattc gttttatgaa
    12181 aaactttgga aaatcgcagg ctttgcacgc tggttttgcc aaagctcaag gcgatgttat
    12241 tatcacgatg gatgcagatt tacaagacag tccagaagaa attccaggtt tgtacgaaat
    12301 gattacagcc caaaactttg acttggtttc aggttggaaa aagaaacgct acgattcggt
    12361 tgttgccaaa aatcttcctt caaaattatt caattgggca gcgagaaaaa cttcgggcgt
    12421 acaattgaac gattttaact gcggattgaa agcctacaaa aacgtggtcg tgaaaaacgt
    12481 ggaagtttcg ggcgaaatgc accgttacat tccagttttg gcaaaaaacg ccggtttcaa
    12541 taaaattggc gaaaaagtag tgcaacacca agctcgaaaa tatggcgaaa ccaaatttgg
    12601 aatggatcgt ttcatcaatg gttttctaga cttgattacc atttggtttt tgtctcgttt
    12661 tggaaaaaga ccgatgcact tgtttggcgc tttgggttcg atgatgttta tgattgggtt
    12721 tgttgcggct ggttacatcg gaatttcaaa attatatcat atgtatagcg gaatgaaata
    12781 tactttggtt accaacaatc cgtggttttt tatagcttta acgacaatgg tattgggaac
    12841 acaattgttc ttggctggat ttctgggcga aattattttg agaaccaaga ataatgagga
    12901 aagatacaag gtttggaaag aaattaatta gcaatttgag gtcacaattt gttacctcaa
    12961 aagacaataa aaaagatatt caaaatttaa aaactaaaat aaatggaaat caaacaaaac
    13021 attttagacg cagtaaacga atggttgaca ccagcattcg acgcacaaac acaagaagcc
    13081 gttatcgaaa tgatgacttc atctccaaaa gatttagaag agagttttta taaaaattta
    13141 gagtttggaa caggcggaat gcgagggata atgggtgttg gaaacaatcg catcaacaaa
    13201 tacaccttag gaaaaagcac gcaaggattg tcgaattatt tgaaaattgc gtttccaaac
    13261 cgacccttaa aagctgttat cgcttttgac tgtcgtcata atagtaagac tttagcaaaa
    13321 gtggttgccg atgttttctc tgccaacggt attcaagtgt atttgttttc agatttgaga
    13381 cctactccag agttgtcttt tgccttgaaa tatttaggtt gccaatgcgg aattgtattg
    13441 acagcctccc acaatccacc agaatacaac ggttacaaag tgtattggga agatggtgga
    13501 caaattgttc ctccgcagga tggtgccatt attgacacta tcgaaaaatt gaattacaac
    13561 caaatcaaat ttgatgctaa cgaagatttg attcaatata tagatgccga aattgaccaa
    13621 gccttcatca aatcgactat cgagaatgct agttttggca cttcgcaagc agccaaagac
    13681 aatttgaata ttgtttttac ttctttgcac ggaacttcta tcactttggt tccagacact
    13741 tttgcgcaag caggatacaa aaacgtgaac attgttgaag aacaaagagt accaaatggt
    13801 gatttcccaa cggtaaaatc tccaaatcct gaagagccag aagctttgac attagcattg
    13861 gcattggcag acaaaaccaa cgctgatatt gtggttggaa ccgatcctga ttgtgaccgt
    13921 ttgggtgttg cagttcgaaa taacgaaggc aaaatgattt tgttgaacgg aaatcaaaca
    13981 atgattttga tgactgcctt tttattggaa gaatggaaaa aagcaggaaa aatcaacgga
    14041 aaacaatttg taggttcgac tatcgtttct actccgatga ttatggaatt ggcttcggct
    14101 tatgaagtag gatttaaagt tggtttgact ggtttcaaat ggattgccaa aatgattaag
    14161 gatttcccag aattggaatt cattggtggt gg
//
LOCUS       DAOSNN010000036        14022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_4371_length_14022_cov_5.202907, whole genome shotgun sequence.
ACCESSION   DAOSNN010000036 DAOSNN010000000
VERSION     DAOSNN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 14022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14022
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4371_length_14022_cov_5.202907"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PLQ70_03280"
     CDS             <1..1105
                     /locus_tag="PLQ70_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility protein RemB"
                     /protein_id="HQE33667.1"
                     /translation="FKTDAYDIPSADANLTFAPAKFLDLQLGYGRNFIGDGYRSIFET
                     DGASPYPYFKINTNFWKIKYTNTYMWLKDVRPEVTLEKTYATKYMANHYLSWNISNRF
                     NLGLFESVIWTDTNNRGFDMHFVNPIIFYRSVEFASSARTGNAVLGLSSKYKWNNNVT
                     LYGQFLLDEFSLNEVKNEKNSWKNKFAYQLGVKYFNAFKVDNLLLQLEYNHVRPYVYS
                     HSDPITNYGHNNQSVGHPWGGNFEELLAIARYHKGRLYADAKITFGTKGFDFVNSGAK
                     SNYGGNIYRDYELDRYADSGVTVGQGNKTNIFITDLQAGYLVNPMTNLKLFGSIIYRN
                     FNPNQNTATVFNESTTWFSFGIRSDVFNWYFDY"
     gene            1256..2158
                     /gene="cyoE"
                     /locus_tag="PLQ70_03285"
     CDS             1256..2158
                     /gene="cyoE"
                     /locus_tag="PLQ70_03285"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044047593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HQE33668.1"
                     /translation="MSSTPNTISIKSIYTDFKAITKAGLAISVVFSSIAGFLLGVSDF
                     HSLHWIILLKLAVGGYCMVGASNAYNQVIEKDLDALMDRTKNRPVASGRMKSNVALVV
                     ASILTVVGIVLLYTINPKTAMFGAISIFLYTSIYTPLKTVTSLSVFVGAFPGAIPFML
                     GWVAATGNFGIEAGTLFLIQFFWQFPHFWAIGWFLYEDYEKAGFFMLPTGKKDKGTAL
                     QIILYTIWLIVASLLPSLGFTGQLFISPVAAVLVFLLGLWMLFYSVKLYQLRTAKAAR
                     TLMLVSVSYITLLQLIYIFDKILR"
     gene            2161..2748
                     /locus_tag="PLQ70_03290"
     CDS             2161..2748
                     /locus_tag="PLQ70_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQE33669.1"
                     /translation="MATTMTAEEHKSRTARSYKLLLLFAMISMIMMFAGLTSAFVVSK
                     SRADWLKDFQLPTAFYFSTAVIIGCSITFHLAKKAIQKDNQKATTSLLLATLALGILF
                     VVLQFMGFGQIIDSGYYFTGAESSITTTFLYIVTVVHLLHLAGGLIALFIIIYKQLKH
                     KYNSNQTLGIELGAMYWHFLDLLWIYLFLFLFFFE"
     gene            2791..3768
                     /locus_tag="PLQ70_03295"
     CDS             2791..3768
                     /locus_tag="PLQ70_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQE33670.1"
                     /translation="MDTVTTANSGDKVWGGGDNQPMGASYGKMMMWFFILSDALTFSG
                     FLAAYGFSRFKFIETWPLADEVFTHFPFMHGNPQPMLYVAFMTFVLIFSSVTMVLAVD
                     AGHQLKKDKVVLYLFLTIIGGLIFVGSQAWEWKNFIKGEYGAVETKGGSLLQFVDTEG
                     HRVALADFAATLPEEREQLTRNKGRWFMDEASLPAYSVAEVQAGFKAHPNILVRTEHI
                     DANKKKTVLSRSESELRLKDASLVVEGANLTRNEYGSKLFADFFFFITGFHGFHVFSG
                     VVINIIIFFNVLLGTYEKRKSYEMVEKVGLYWHFVDLVWVFVFTVFYLI"
     gene            3789..4139
                     /locus_tag="PLQ70_03300"
     CDS             3789..4139
                     /locus_tag="PLQ70_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HQE33671.1"
                     /translation="MSHAHVSNTKRIWTVFGLLSIITIVEVALGIAKPGFLHNNNLVG
                     VNLLNWIFIILTLVKAYYIVYAFMHMEGERKSLQYAVIFPLIFLVIYLLFILLTEGHY
                     IFEVFKNSTIKWNF"
     gene            4215..4856
                     /locus_tag="PLQ70_03305"
     CDS             4215..4856
                     /locus_tag="PLQ70_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33672.1"
                     /translation="MKKNIVLFILFVLPIVAYLFFASGVNSFTKLPVITPKVADFGNW
                     KSLKNEKVSLNGKITILGFSGTNLLKNRGNYFNLNEKIYQRYHTFKDLQFVVVCPLGT
                     EIQAAKLMDNLDDFTDVSQWHFIFTSPEEITKYYNQLKLVGKLDADLGTPNVYILDKE
                     RNLRGRKEKKGYKEGYSTFHPADLSNEMLDDFKVILYEYRAALKKNHNATKQL"
     assembly_gap    4892..4991
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5051..5599
                     /locus_tag="PLQ70_03310"
     CDS             5051..5599
                     /locus_tag="PLQ70_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HQE33673.1"
                     /translation="MEQKSKSIEQKFSTFIILVSILIPVAVAVLLSVRLKDFGINVEP
                     LHFLPPIYAAINGLTAIVLVAGVWAIKNGNRQLHQRLMTTAIALSVVFLVMYIAYHLT
                     TDSTKFGGEGLVRPIYFFILISHIVLSIAVIPLVMITYVRALAEKFDAHKKIAKITFP
                     IWLYVAVTGVVVYLMISPYYVQ"
     gene            5589..5846
                     /locus_tag="PLQ70_03315"
     CDS             5589..5846
                     /locus_tag="PLQ70_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33674.1"
                     /translation="MFSKVRSEKRGAGIGKLVLFGICFLFFVMSSYAQCAMCRAALAG
                     EDNKVKAQAVNDGIVYLMIIPYVLVAGIGYYIYRMRKKKNN"
     gene            complement(5894..7291)
                     /locus_tag="PLQ70_03320"
     CDS             complement(5894..7291)
                     /locus_tag="PLQ70_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HQE33675.1"
                     /translation="MLKFLPFLFAILIVSMIASCSTSNKIAALKPEPNDASPLVYETA
                     PSFINLPISVKLKDIENQTNTILNGLIYEDNNIEDDNIEMKIWKLAPISITNENGKLK
                     TILPLKAAIKYRIGTKTMGVELYNYKEFNLNGVVTLTSDVALTNWKLNTKTELKSLDW
                     NESPTMVVFGKNMPVTYLINPTVKLFRSKIESKIDAAIEKSMDFKPNVLAALEKICSP
                     FQMSETYESWLRITPIEIYSTDAVLKNDTFLMDMGMKCTMTTLVGTKPESKFDASKII
                     LKPVTKIPNQISANIAAVSTFAEAAKIMNKNFAGQEFGSGSKKVTVQKVDIWHKNDKM
                     VIALDVIGTVNGTIYLTGFPQYNEQTKEIFFDKLDYALDTKNKLMRTANWLAQGLVLR
                     KIQESCRYSIVPNLEEGKQTMANYLKNYSPMPGVFVNGKMDNLQFQKVQLTNKAIIAF
                     IKVNGTVNVAVDGLK"
     gene            7465..8166
                     /locus_tag="PLQ70_03325"
     CDS             7465..8166
                     /locus_tag="PLQ70_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE33676.1"
                     /translation="MIEIKDLHKSYKMGHSELHVLKGINFNIKEGELVAIMGSSGSGK
                     STLLNILGILDEADSGDYVLDNVPIKKLNETIASKYRNQFLGFVFQSFNLINYKSALD
                     NVAMPLYYKGVKRKERNEIAMKYLEKVGLVSHSHHLPSELSGGQKQRVAIARALASNP
                     KVLLADEPTGALDTLTSYEVMELIQGINDEGKTILIVTHEPDIAAMCKRNVVLKDGVI
                     IDDKYVEQVRASSYV"
     gene            8159..9406
                     /locus_tag="PLQ70_03330"
     CDS             8159..9406
                     /locus_tag="PLQ70_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE33677.1"
                     /translation="MFNIERWQEIFEAIAKNKLRTFLTGTSVASGIFILVILLGAGKG
                     LENGIAKQFENDADGVIWIWAGTTTKEYKGLNPGRRIQLRNSDFEISKQKFEDQIDKK
                     SSKFSIWNGNIVYGKETGNYDCQGVSPDHQAIEKVTITEGRFVNLLDIKNNRKVAVIG
                     QKLKNDLFKEKNAIGEQIAISNVKFEVVGVFTDPGGEREETRTYLPVTTVQQVFGSGS
                     DKINDMSFTLHKKDTYEEALAESKKFTDGVSQLLKGKNTVAPDDTSAIGINSTVENIK
                     QFYDLNLYIRLFFWWVGICTIIAGVVGVSNIMLIIVKERTKEIGIRKALGATPLSIVG
                     MILHESIFITTIAGFVGLLSSLALLELVGPQIKSDYFLNPEVDFTIALSTLILLVIAG
                     ALAGFFPAYRAAKIKPIVALRDE"
     gene            9409..10677
                     /locus_tag="PLQ70_03335"
     CDS             9409..10677
                     /locus_tag="PLQ70_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE33678.1"
                     /translation="MLDRDKWAEILEALTANTFRTLLTAFGVFWGIFILVILLAAGNG
                     LENGVKQGFSGMATNTMFMWTQTTSKPYKGLPKSRQFSFQNSDVPALKEKFPDLLYVS
                     PRNQLGGFRGGNNVVRGTKTAAFSVYGDYPEYVKQESMDIVKGRFVNYQDIELSRKVA
                     VIGQGVIDGLYDKTEEVIGSYIKVNGVNFMVVGIYKKKANGNDGNAEEQQKNIFTPFT
                     AFQQAFNFGDKVGWMAITAKDEASITELKPQILEFMKSRHSISPDDERAIGNFDLYEQ
                     FAKILGLFSILRFIAYFVGTLVLLSGVIGISNIMLIVVKERTKEIGIRRALGATPMAI
                     RSQILLEAIFLTISSGMLGIAAATGVLILVNNILDSMPSEGSMFANPSVDLGVVFFAL
                     VILVGSGLLAGLIPAQTAIKVKPVDALRTE"
     gene            10717..11832
                     /locus_tag="PLQ70_03340"
     CDS             10717..11832
                     /locus_tag="PLQ70_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQE33679.1"
                     /translation="MKKGVTITILIFIAIVFFGALYYLYAKNQESPVVFQTDKTEVKT
                     IVKNTIATGNIVPDEEVLIKPNISGIIEEIYIEAGQTIKAGDLIAKIKVVANVSSLNN
                     TQNQVQTAKINLDNQEKLFQRQKTLFDKGVISANDFDAAQVAYKQAKQNYVAAQKSND
                     IVKTGTTSGMGNYANTLIRSTVNGMVLEVPVKVGNQVIESNNFNEGTTIASVADVGKM
                     IFVGKIDESEVGKIKVNLPIEITVGAIENKKFDAVLTDIAPKGKTENGAIQFEIKASL
                     SNRDDTFIRAGLSANASIILEKADKVMAVKEALIQFDAKTQKPFVEVETANQKFVRKD
                     LVLGVSDGIYVEIKSGLKSTDKIKIWNQGLNTEEPKP"
     gene            11915..13273
                     /locus_tag="PLQ70_03345"
     CDS             11915..13273
                     /locus_tag="PLQ70_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE33680.1"
                     /translation="MKKIKSISLVIFLLFGLSIQAQTKTWTLEECVKYAIDNNISIKQ
                     TELDTKTADIDKKGAIGNFIPSLNSNASHSWNVGLNQDPTTGLLRNQTTQYSSMGVGV
                     GVDIYKGMQNQNTLRRANLSIIAAKYQLTKMQEDVALNVANAFLQVLFNKENLKVQKE
                     QKANNEKQLSRSEELVKAGSVPKGDLLDIKATVASDGQKVVAAENALLISKLSLAQLL
                     QLKEFYDFDVTDETTVKDENNILAQTPLAIYDKAKEQRTELKIAKTNLEIAEKNVAIA
                     KGGYQPTLQGFYNINTRISYADSYNFQTGLSSPAPPFSQQFADNRGQAFGAQLNIPIF
                     NGWSAKNNVERSKVNLEKSKIAVEQEELALQRNVYTAFTDAKGGLNAYESALSALEAR
                     QEAYNYAKEKYAVGMMNSFDFNQSQTLFSAAQSEVLRTKYDYIFKIKILEFYFGIPII
                     KN"
     gene            13280..>14022
                     /locus_tag="PLQ70_03350"
     CDS             13280..>14022
                     /locus_tag="PLQ70_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQE33681.1"
                     /translation="MSKKTIYLLIGGAVALIALLIALSKTGVIGNKDKGKSIEIAKVN
                     SSTIVETVSATGKIQPEIEVKISSEVSGEIIALNVIEGQVVKKGDLLVKINPDLYTSG
                     YNRTLSNLSGTKANLSQADASFKEAKANYDRNKTLFDKGIISKSDWDKSVASFEVAKA
                     NKQSAYFNVQSASATVNEAKDNLGRTTIYAPADGTISVLNVELGERVLGTQQMAGTEI
                     LRVANLNNMEVEVDVNENDIVKIKVGNEA"
BASE COUNT         4636 a         2160 c         2641 g         4485 t
ORIGIN      
        1 ttttaaaact gatgcttatg atattccttc ggctgatgcc aatttgactt ttgctcctgc
       61 taaatttcta gatttacaat tgggttatgg tcgcaatttt attggtgacg gttaccgttc
      121 tatcttcgaa actgacggag caagtccgta tccttatttc aaaatcaaca ccaatttttg
      181 gaaaataaaa tacaccaata cctatatgtg gctcaaagat gtgcgtccag aagtcacttt
      241 agaaaaaaca tatgctacca agtatatggc caatcattat ttgagttgga atatttctaa
      301 tcgctttaat ctaggtcttt ttgaatcggt gatttggaca gatactaata atcgaggttt
      361 tgatatgcat tttgtaaacc caattatttt ttatcgttca gtcgaatttg cttcatcggc
      421 aagaactgga aatgctgttt tgggcttgag ttctaaatac aaatggaata ataatgtaac
      481 cctttatggg caattcctgt tagacgagtt ttctctaaat gaagttaaaa acgaaaaaaa
      541 cagctggaaa aataaatttg cttatcaatt gggtgtgaaa tatttcaatg cattcaaagt
      601 agataattta ttattgcaat tagaatacaa tcatgtgcgt ccttatgtgt attcgcatag
      661 cgacccgatt accaattacg gacacaataa tcaaagtgta gggcatcctt ggggaggaaa
      721 ttttgaagaa cttcttgcca ttgctcgcta tcacaaaggg agattgtatg ccgatgcaaa
      781 aattactttt ggtactaaag ggtttgattt tgtcaattca ggagccaaat ccaattacgg
      841 aggtaatatt tacagagatt atgagctaga tcgttatgct gacagtggag taacagttgg
      901 tcaaggaaac aaaaccaata ttttcatcac cgatttacaa gcgggttatt tggtcaatcc
      961 aatgacgaat ttgaaacttt ttggtagtat tatttacaga aacttcaacc caaatcaaaa
     1021 tacagccaca gtctttaacg aaagcaccac ttggttttct ttcggaattc gctcagatgt
     1081 gtttaattgg tattttgatt attaaaattt cgggaatatt tcgggtaaaa aggtataagc
     1141 aaactttttc aattataaaa tgataaaact cctgttttaa gaattatttt tatttgccaa
     1201 atcccttttg ataaactact tttgcaaaag ttttaaaaaa caaacaaaac aaacattgag
     1261 tagtacgccc aataccattt ctatcaaatc aatttatact gatttcaaag caattactaa
     1321 agctggttta gccattagtg ttgttttctc atcaatagca ggatttttgt tgggtgtttc
     1381 tgattttcat tctttgcatt ggattatttt attaaagcta gctgttggtg gttattgtat
     1441 ggtgggtgcg tcaaatgcct ataatcaagt gatcgaaaaa gatttggatg ctttgatgga
     1501 tagaactaaa aaccgtcctg tagcctcggg aagaatgaag tcgaatgtgg cattggttgt
     1561 tgccagtatt cttacggttg ttggaattgt tttgctttat acaatcaatc ctaaaacagc
     1621 gatgtttggt gcgatttcaa tatttttata tacaagtatt tatacgcctt tgaaaacggt
     1681 gacttcgttg tcggtttttg tgggtgcttt tccgggagcg attcctttta tgttaggttg
     1741 ggttgccgca acgggcaatt ttggcattga agctggaacc ttatttctga ttcagttttt
     1801 ttggcagttt cctcattttt gggcaatcgg ttggttttta tatgaagatt acgaaaaagc
     1861 agggttcttt atgttgccta cagggaaaaa agataaagga acggccttgc aaatcatttt
     1921 atacaccatt tggcttattg tggcgtcgct tttgccatct ttaggtttta caggacaatt
     1981 gtttatctcg ccagtggcag cagttttagt ctttttgctt ggtctttgga tgctttttta
     2041 ttcggtaaaa ttgtatcaat taagaacagc aaaagcagca agaacattga tgttagtaag
     2101 tgtttcttac attacattat tgcaattaat ttatatattt gacaaaattt taagatagat
     2161 atggcaacga caatgaccgc cgaagagcat aaatcaagaa cagcaagatc gtacaaattg
     2221 ctgctattgt tcgctatgat cagtatgatt atgatgtttg caggacttac aagtgctttt
     2281 gtggtaagta aatcaagagc cgattggttg aaagattttc aattgccaac tgcattttat
     2341 ttcagtacag cagttattat tggttgtagc atcacttttc atttggccaa gaaagccatt
     2401 caaaaagaca atcaaaaagc aacaacatcc cttcttttgg cgactttagc tttagggatt
     2461 ttatttgtag tattgcaatt tatgggcttt ggacaaatta tcgatagcgg atattacttc
     2521 acaggtgccg aaagttcgat tacgactaca tttttataca ttgttacagt agttcacttg
     2581 cttcatcttg ctggcggatt aattgcgctt tttattatca tttataaaca actaaaacac
     2641 aaatacaatt caaatcaaac actaggaata gaactaggtg ccatgtactg gcactttcta
     2701 gatttgttgt ggatttattt atttttattt ttatttttct ttgaataaga aaaaattgta
     2761 gatttgaaaa cttttaacta atattttttt atggacacag ttacaactgc gaatagcgga
     2821 gacaaagttt gggggggtgg agacaaccaa ccaatgggag ccagttatgg taaaatgatg
     2881 atgtggtttt ttatcctatc agatgcttta accttttcgg gatttcttgc cgcttatgga
     2941 ttttcaagat ttaaatttat cgaaacttgg cctttagccg atgaggtatt tacacacttt
     3001 ccgtttatgc acggaaatcc tcagcccatg ttatatgtgg cattcatgac atttgttttg
     3061 attttttcat cagttacaat ggtgcttgct gttgatgctg gtcatcaatt aaaaaaagat
     3121 aaagtagtcc tttatctgtt tttaacgatt attggtggtt taatattcgt tggctcacaa
     3181 gcttgggagt ggaaaaactt tattaaaggc gaatatggtg cagtagaaac caaaggagga
     3241 agtttacttc agtttgttga cacagaaggt caccgcgttg cacttgctga ttttgctgct
     3301 actttacctg aagaaagaga gcaattgaca cgaaataaag ggcgttggtt tatggatgaa
     3361 gcttctttgc cagcctattc agttgccgaa gttcaagctg gtttcaaagc gcatccaaat
     3421 attttggtaa gaaccgaaca tattgatgcc aataagaaaa agacagttct ttctagaagt
     3481 gaatcagaat tacgtttgaa agatgcgagt ctagttgttg aaggtgcaaa ccttactaga
     3541 aacgaatatg gtagcaagtt atttgctgat ttctttttct ttatcacagg tttccacggt
     3601 ttccacgtat tttctggagt agttatcaat atcattatct tctttaatgt attacttgga
     3661 acttacgaga aaagaaaaag ttatgaaatg gtagaaaaag taggactata ttggcacttc
     3721 gttgatttag tttgggtttt tgtattcaca gttttctatc taatttaatt cacaaaaaag
     3781 atattattat gtcacacgca cacgtttcta acacaaaaag aatttggacc gtttttggtt
     3841 tattatccat aataacaatt gttgaagtag ctttggggat tgcaaaacct ggttttttgc
     3901 ataacaataa tttggtaggt gtaaatttgt taaactggat attcatcatt ttgacattag
     3961 taaaagctta ttatattgta tatgctttta tgcatatgga aggggagaga aaatcattgc
     4021 aatatgcagt tatttttccg cttattttcc tagtgattta tttgcttttc atccttttga
     4081 cagaaggaca ttatattttc gaggtattta aaaactctac cattaagtgg aatttttaac
     4141 aaaatattaa ttcaataaaa aggtgcgctt tgcgtgcctt tttttatttt tgcacactat
     4201 acttcggcac aattatgaaa aaaaatattg ttctcttcat tctttttgtt ttgccaattg
     4261 ttgcctatct tttttttgca tcaggtgtaa acagtttcac aaagctaccc gtcattactc
     4321 ccaaagttgc tgattttggc aattggaaat ctttgaaaaa tgaaaaagtt agtttgaatg
     4381 gcaagattac aattcttggc ttttcgggaa ctaatttgtt aaaaaataga ggaaactatt
     4441 ttaacttaaa cgaaaaaatt tatcagcgat atcacacttt caaagatttg caatttgtgg
     4501 tcgtttgtcc tttaggaacc gaaattcaag ctgcgaaatt aatggataat ttggatgatt
     4561 ttacagatgt tagtcaatgg cattttattt ttacatcgcc agaagaaata actaaatatt
     4621 acaaccaatt aaaacttgtt gggaaattgg atgcagattt gggaacacct aatgtttata
     4681 ttcttgacaa agaaagaaat cttagaggac gtaaagagaa aaaaggatac aaagaagggt
     4741 acagtacttt tcatccagca gatttgagca acgaaatgct ggacgatttc aaagttattc
     4801 tctacgaata tcgtgctgca ctcaagaaga atcataatgc aacaaaacaa ctttaatctt
     4861 ttgaaataat gcttaaaaac aaatcataca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn ngataaaaaa ggagttagag acattcaaca agatatagca attttactaa
     5041 aagaataaaa atggaacaga aatcaaaatc aattgaacaa aaattcagca catttataat
     5101 actagtttct attttaattc cggttgcagt tgcggttctt cttagtgtta ggctaaaaga
     5161 ttttgggata aatgttgaac cacttcattt tctacctccc atttatgcgg ccataaacgg
     5221 tttaactgct atagttttag tagctggagt ttgggcaatc aaaaacggaa atagacaatt
     5281 acatcaacga ctaatgacaa cggctattgc gctctcggtt gtttttttag tgatgtatat
     5341 tgcctatcat ttaactactg attcaactaa atttggaggc gaaggattag taaggccaat
     5401 ttatttcttt attttaatat cgcatattgt tttgtccatt gcggttattc cattagtaat
     5461 gattacttat gttcgcgctt tggcagaaaa attcgatgca cataaaaaaa ttgcaaaaat
     5521 cacattccca atttggttgt atgttgctgt aactggagta gttgtttatt taatgatttc
     5581 gccttattat gttcagtaaa gttagaagtg agaagcgggg agcggggatc gggaagctgg
     5641 ttctttttgg aatttgcttt ttgttttttg taatgtcatc ctatgctcaa tgcgctatgt
     5701 gccgagcagc tttggcaggt gaagataata aagttaaagc ccaagctgta aatgatggta
     5761 tcgtttattt gatgataatt ccttatgtgc ttgtggctgg aattgggtat tacatttatc
     5821 gaatgaggaa aaagaagaat aattaatttt tcagagtcct actttccaac acctaagacc
     5881 ctgaacccaa tgcctatttc aacccatcaa cagcaacatt caccgtgcca tttactttta
     5941 taaaagcaat aatggctttg ttggtcaatt gcactttttg aaattgtaaa ttgtccattt
     6001 ttccattgac aaaaacacct ggcattggcg aataattttt taaatagttt gccattgttt
     6061 gttttccttc ttcaagattt ggaacgatag aatagcgaca gctttcctga atttttctta
     6121 aaactaatcc ttgtgcaagc caattggcag ttcgcattaa tttatttttg gtatccaaag
     6181 cataatctaa tttatcgaaa aaaatttctt tggtttgctc attatattgt gggaaaccag
     6241 ttaaataaat agttccgtta acagttccta taacgtccaa agcaataacc attttgtcgt
     6301 ttttatgcca gatatcaact ttttgaacag ttactttttt acttccagaa ccaaattctt
     6361 gacctgcaaa attcttgttc ataatttttg cagcttcggc aaaagttgaa acggctgcaa
     6421 tattagctga aatttgattt ggaattttag ttacaggttt cagtattatt ttggacgcat
     6481 caaattttga ttctggtttc gtgccaacca aagtggtcat ggtgcatttc attcccatat
     6541 ccatcaaaaa agtatcgttt ttgagtactg catctgtcga gtaaatttca attggagtaa
     6601 ttcgcagcca actttcgtat gtttcgctca tttgaaaagg cgaacaaatt ttttctaaag
     6661 ctgccaaaac attaggtttg aaatccatcg atttttcaat tgctgcatca attttacttt
     6721 caattttaga tctaaaaagc ttcacagtag gattgatgag ataggttaca ggcatatttt
     6781 tgccaaaaac caccatcgtt ggactttcat tccaatccaa agattttagt tctgttttgg
     6841 tattgagttt ccagtttgtt aaagccacat cacttgttaa agtaactacg ccatttaagt
     6901 taaattcttt gtagttgtag agttcaactc ccatagtttt tgtgccaatt ctgtatttta
     6961 tagctgcttt taatggtaaa atggttttta attttccgtt ttcatttgta attgaaatag
     7021 gggccaattt ccagattttc atctcgatat tatcatcttc gatgttgttg tcttcataaa
     7081 ttaaaccgtt caagatagta ttggtttgat tttcgatgtc ttttaatttg acgcttatgg
     7141 gcaaattaat aaaagaagga gcggtttcat acaccaaagg actggcatca tttggttctg
     7201 gttttagtgc cgcaatttta ttggaggtag agcaactcgc aatcatggaa acaattagaa
     7261 ttgcaaatag aaaaggaagg aattttagca ttatattttt tgtttgaaga aacaaaaata
     7321 ataaaacaaa tatatttttt ggctttgcgt gtaacatatt aatagaaatt aggtcttata
     7381 tcaaaataga atgaaacctt taacgttttc ttgttaaaac tacatgtaaa gaatttctaa
     7441 atttgtttaa aaaaataatc taaaatgatt gaaattaaag acttacataa atcatacaaa
     7501 atggggcatt ccgaattgca tgtgctaaaa ggaatcaatt tcaacatcaa agaaggtgaa
     7561 ttagtagcca ttatgggttc gtcaggttca ggaaaatcaa ccttattaaa tattcttgga
     7621 attcttgacg aagctgattc tggcgattat gttttggata atgttccaat aaaaaaactc
     7681 aacgaaacta tagcttctaa ataccgaaac cagtttttgg gttttgtttt ccaatctttc
     7741 aatttaatca attataaatc ggctttagac aatgtggcta tgcctttgta ttataaagga
     7801 gtaaaacgaa aagaacgcaa cgaaattgca atgaaatatc tagagaaagt aggtttggtt
     7861 tcgcattcac atcacttgcc aagcgaactt tctggtggac aaaaacaacg tgtggcgatt
     7921 gcaagagcat tagcttcaaa tccaaaagtt ttattggccg atgagccaac aggagcttta
     7981 gataccttga cttcttatga agtaatggaa ctcattcagg gaatcaacga tgaaggaaaa
     8041 accattttga ttgtaaccca cgaacccgat attgccgcaa tgtgcaaaag gaacgtggtt
     8101 ttgaaagatg gagtaatcat cgatgataaa tatgtagaac aagtaagagc ctcttcttat
     8161 gtttaatatt gaacgctggc aggaaatatt tgaggccatt gccaaaaata aactgagaac
     8221 ctttctcacg ggaacttctg tggcctcggg gatttttatt ttggtcattc ttctcggagc
     8281 aggaaaagga ctcgaaaacg gaatcgccaa acaatttgaa aacgatgccg atggcgtaat
     8341 ttggatttgg gcaggaacca caaccaaaga atacaaagga ctaaatcctg gaagacgcat
     8401 tcaattgcga aacagtgatt ttgaaatatc gaaacaaaaa tttgaagatc aaattgataa
     8461 aaaaagttcg aaattcagta tttggaatgg caatatcgtt tacggaaaag aaaccggaaa
     8521 ctatgactgt cagggggttt ctccagatca tcaagccatc gaaaaggtaa ccattacaga
     8581 aggaaggttt gttaatttac ttgatataaa gaataataga aaagtagctg ttattgggca
     8641 gaaattaaaa aatgatttgt ttaaagaaaa aaatgccatt ggagaacaaa tcgccattag
     8701 taatgtcaaa tttgaagtag ttggcgtttt tacagaccca ggtggtgagc gagaagaaac
     8761 tagaacctat ttgcctgtca caacagtgca acaagttttt ggaagcggaa gtgataaaat
     8821 aaacgatatg tcttttacat tacacaaaaa agacacttat gaagaagctc ttgctgaatc
     8881 gaagaaattt actgacggag ttagtcaatt gcttaaaggc aaaaataccg tcgctcctga
     8941 tgatacaagt gctataggaa ttaatagtac agtcgaaaat atcaaacaat tttatgattt
     9001 gaatttgtat ataagattgt ttttttggtg ggtgggaatt tgcaccatca tagccggagt
     9061 tgtgggagta agtaatatta tgttgattat tgtaaaagaa cgaaccaagg aaattggaat
     9121 tagaaaagcc ctcggagcaa caccactttc cattgtgggg atgattttgc acgaatctat
     9181 ttttattacc acaatagctg gatttgttgg acttttgtca agcttggcgc tattggaatt
     9241 agttggacca caaataaaaa gcgactattt tttaaatcca gaagtcgatt ttaccatagc
     9301 attaagcaca ttgatattgt tagtaattgc aggagctttg gctggttttt ttccagcata
     9361 tcgagcagcc aaaattaaac ctattgtagc acttagagac gaataattat gttggataga
     9421 gataaatggg ctgagatttt agaagcattg acagcaaata cttttcggac attgcttact
     9481 gcttttggtg ttttctgggg gatattcatc ttggtaattt tacttgccgc aggaaatggg
     9541 ctggaaaatg gagtgaaaca aggattttcg ggtatggcaa ccaatacgat gtttatgtgg
     9601 acacaaacca cttcaaaacc gtataaaggg ctaccaaaat cgagacagtt tagttttcaa
     9661 aatagtgatg ttcctgcttt aaaagaaaaa tttccagacc ttttgtatgt ttcgcctaga
     9721 aatcaattag gaggttttag aggtggaaat aatgtggtta gaggaacaaa aacagctgct
     9781 tttagtgttt atggagacta tcctgaatat gtgaagcagg aatctatgga tattgtaaaa
     9841 ggacgttttg taaattatca agacattgag ttgagtcgca aagtggccgt aatcggtcag
     9901 ggtgtaattg acggattgta tgataaaaca gaggaggtta taggttctta catcaaagtc
     9961 aatggtgtga attttatggt tgttggcatt tataagaaaa aagcaaatgg taatgatggc
    10021 aatgccgagg aacagcaaaa aaatattttc acgcctttca ccgcttttca acaggctttt
    10081 aattttggtg acaaagtggg ttggatggca attaccgcca aagatgaagc ttcaataacc
    10141 gaattaaaac cccaaattct agagtttatg aaatccagac attccataag tccagatgat
    10201 gaaagagcca taggaaattt tgatttgtac gagcaatttg caaagatttt gggattgttt
    10261 tccatactta gatttattgc ttatttcgtt ggaaccttgg ttttgctttc gggagttatt
    10321 gggatttcga atattatgct cattgtggtg aaagaacgca ccaaagaaat aggaattaga
    10381 agagctttgg gggcaacacc tatggctatt cgatcccaaa tattattaga agctattttc
    10441 ctgaccatat cttctggaat gttaggcatt gccgccgcaa caggagtttt gatacttgtc
    10501 aataatattt tagattcaat gccaagcgag gggtcaatgt ttgccaatcc aagtgtcgat
    10561 ttgggagtag tgttttttgc cttagtaata ttagtgggtt ctggattatt agccggactt
    10621 attcccgctc aaaccgctat aaaagtaaaa ccagttgacg cattacgaac cgaataaaac
    10681 agcaatcaat ataaatcgaa ttaaacaatc atcaatatga aaaaaggagt aaccataacc
    10741 attttaatct ttatagccat tgtatttttc ggtgcgctct attatttgta tgccaaaaac
    10801 caggaatctc ctgttgtttt tcagacagac aaaaccgaag ttaaaactat tgttaaaaac
    10861 acaatcgcaa caggaaacat tgttccagac gaagaagtgc tcataaaacc aaatatttca
    10921 ggaattatcg aagaaattta tatcgaagct ggacaaacga ttaaagctgg cgatttaatt
    10981 gcaaaaataa aagtggttgc taatgtttct agtttaaaca atacacaaaa ccaagttcaa
    11041 acggctaaaa taaatttgga taaccaagaa aaattgttcc aaagacaaaa aaccttgttt
    11101 gataaaggag tgatttcagc caatgatttt gatgcagctc aagttgccta caaacaagcc
    11161 aagcaaaatt atgtggcagc ccaaaaaagc aacgatattg taaaaacagg tacgacttca
    11221 ggaatgggta attatgccaa cacattaatt cgttctaccg taaacggaat ggtgcttgaa
    11281 gtacctgtaa aagtaggaaa tcaagtaatc gaaagtaata actttaacga aggaacgact
    11341 attgctagtg ttgccgatgt tggaaaaatg atattcgttg gaaaaattga cgaatccgaa
    11401 gttggaaaaa taaaagtaaa cttgcctatc gaaattactg ttggagccat cgaaaacaaa
    11461 aagtttgatg ccgttttaac cgatattgct ccaaaaggaa aaaccgaaaa cggagccata
    11521 caattcgaaa taaaagcttc gctatccaac agagacgata cttttatcag ggcaggtctg
    11581 agtgccaatg cctcgattat tctagaaaaa gccgataaag tgatggctgt aaaagaagct
    11641 ttaatacagt ttgatgccaa aacacaaaaa ccgtttgtag aagttgaaac tgcgaatcaa
    11701 aaatttgtta gaaaagatct tgttttaggt gtaagcgatg ggatttatgt agaaataaaa
    11761 agcggtttga aatctaccga caaaataaaa atatggaatc aaggcttgaa tacagaagag
    11821 ccaaaaccgt aagtttagct tttaaaaaga atttcgattt taaaaaaaat gtaaaatttg
    11881 tgtagtacct ttgctatgct ttcattccaa aaatatgaaa aaaataaaaa gtatcagtct
    11941 cgttatattc ttgctgtttg gcttgtcgat tcaggctcaa actaaaactt ggacattaga
    12001 agaatgcgtt aaatacgcca tagacaacaa tatttctatc aagcaaaccg aattagatac
    12061 taaaacggcc gatattgaca aaaaaggagc catcgggaat tttattcctt cgttaaactc
    12121 taatgcttcg cattcatgga acgtgggttt aaatcaagac ccaactactg gacttttaag
    12181 aaatcaaacc actcaatact cctcaatggg agttggtgtt ggtgttgata tttacaaagg
    12241 aatgcaaaat caaaatacac ttcgaagagc taatctttcc ataattgcgg ctaagtatca
    12301 gttgacaaaa atgcaggaag atgtcgcttt aaatgttgct aatgcctttt tgcaagtgct
    12361 ttttaataaa gaaaatttaa aagtacaaaa agaacaaaaa gccaataacg aaaagcaatt
    12421 gtctcgttca gaagaattgg taaaagcagg ttctgttcca aaaggagatt tattagacat
    12481 aaaagcaact gtagcatccg atggccaaaa agtagtggct gctgaaaatg cgttgttgat
    12541 ttcaaaatta tctttggctc aattattaca gttgaaagaa ttttatgatt ttgatgtaac
    12601 agatgagact acggttaaag atgaaaataa tatcttggct caaactccat tggctattta
    12661 tgataaggca aaagagcaaa gaactgaatt gaaaattgct aaaactaatc ttgaaattgc
    12721 cgagaaaaat gtagctattg ccaaaggagg ttatcaacct acattgcaag gtttttacaa
    12781 tattaatact cgaatttctt atgctgattc gtataatttt caaacaggtt taagttctcc
    12841 agcacctcca ttttcacagc aatttgctga caatagaggt caagcttttg gggcacaact
    12901 aaatatccct atttttaatg gttggtctgc aaaaaataat gtagaacgat ctaaagtaaa
    12961 tttagaaaaa tcaaaaattg cggttgaaca agaagaattg gctttgcaaa gaaatgtcta
    13021 tactgcattt acggatgcta aaggaggttt aaatgcctat gaatcggctc tttcagcttt
    13081 agaagcaaga caagaggcct acaattatgc taaagaaaaa tacgcagttg gaatgatgaa
    13141 ttctttcgat tttaaccaat cgcaaacttt attttcagca gcacaatctg aagtgttgag
    13201 aactaaatac gattacattt tcaaaataaa aatactcgaa ttctattttg gaattccaat
    13261 cataaaaaac taattcatta tgtcaaaaaa aaccatttat ttattaatag gaggagcagt
    13321 tgcactaatt gctttattaa tagcactttc taaaacagga gttattggaa ataaagacaa
    13381 aggaaaatct attgaaatcg caaaagtcaa ttcttcaaca atcgtcgaaa ccgtttctgc
    13441 tacggggaaa atccaacccg aaatcgaagt gaaaatttcc tcggaagtgt cgggcgaaat
    13501 tattgctttg aatgtaattg aaggccaagt agttaaaaaa ggcgatttgt tagtaaagat
    13561 aaatcccgat ttgtacacat caggatataa tcgaacactt tcgaatttgt cgggaactaa
    13621 agcaaattta agccaagccg atgcttcgtt taaagaagcc aaagccaatt atgaccgaaa
    13681 taaaacatta ttcgacaaag gaattatttc taaatcggat tgggataaat cagtagcttc
    13741 ttttgaagtg gccaaagcca acaaacaatc ggcttatttc aatgtgcaat ctgcttcggc
    13801 aacagtaaat gaagccaaag acaatctagg acgaaccacc atttatgcac ctgccgatgg
    13861 aacaatttct gtacttaacg tggaattggg cgaaagagtt ttgggaaccc aacaaatggc
    13921 aggaaccgaa attcttcgag tagccaattt aaataatatg gaagttgaag tagatgtaaa
    13981 tgaaaacgac atcgtaaaaa taaaagtagg aaacgaagcc aa
//
LOCUS       DAOSNN010000037        13882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_4439_length_13882_cov_5.717148, whole genome shotgun sequence.
ACCESSION   DAOSNN010000037 DAOSNN010000000
VERSION     DAOSNN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4439_length_13882_cov_5.717148"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="rsmG"
                     /locus_tag="PLQ70_03355"
     CDS             <1..540
                     /gene="rsmG"
                     /locus_tag="PLQ70_03355"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQE33682.1"
                     /translation="AKINVISRKDIDELYTKHILHSLGIAKVVKFEPGTYVLDVGTGG
                     GFPGIPLAILFPETRFYLIDVIAKKIKVVQAVVDALELKNVKAEQKRAELVKGDFDFI
                     VSRAVTNMPDFVSWVKTKIKKQHKHELKNGILYLKGGDLTEELKDFPKATEYNLSDFF
                     ENEFFETKKVVYLPLKFQA"
     gene            564..1058
                     /locus_tag="PLQ70_03360"
     CDS             564..1058
                     /locus_tag="PLQ70_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33683.1"
                     /translation="MKKFTDIFITELNIEKKCFVDKFSNSIESSRIDFMSEFLNSLSL
                     KKSNKKLIGKLKNDSFELKINSTMNILNYSTAVAKGIITDSKNGIKIETRIKGFDYRF
                     IPLYIILSIVFIVSLIGLFTDFKFIFGLIISSGIFLFTMNNMLDDIQKLKIELTNEMK
                     SISE"
     gene            1211..2332
                     /locus_tag="PLQ70_03365"
     CDS             1211..2332
                     /locus_tag="PLQ70_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQE33684.1"
                     /translation="MKKISRRKFVHNFGLGVGSTAVIASLPSFMTLSENSKNLYDGKK
                     LNIALCGLGRYAGYLAEGLEISKYCRLAGVVTGTPSKALDWGKKYNIPEKNIYNYQNF
                     DEIIHNKDIDLVYVVLPNAMHKEFAIRAAKAGKHVIVEKPMAITASDCQEMIDACNKA
                     GVQLAVGYRLHYEPYNLEMKRLGQEKVFGQVRLIETSLGYNTGDTIDWHSKKALSGGG
                     PLINLGIYCVQASRYILGEEPISVTAQFGPVTNKIKFAEVEESITWQLNFPSGAVCNS
                     ASSYNCGVDRLFASAEDGFFELSPAISYGPFKGRTSNGDLNFPEINQQQTQLDEIGKL
                     ILENKKLPSHITGEEGLKDVTILQAIYEAARTGKKVSLV"
     gene            complement(2421..4049)
                     /gene="pruA"
                     /locus_tag="PLQ70_03370"
     CDS             complement(2421..4049)
                     /gene="pruA"
                     /locus_tag="PLQ70_03370"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HQE33685.1"
                     /translation="MPKGFFNVPKAVNEPVKSYAPNSPEKAAVLAAYKKMWDSKIDVP
                     LYIGSEEIRTGNTRNMTAPHDHKHVVGTYHLAEKSHIEKAIANALEAKTKWANMAWEQ
                     RAAIFLKAAELIAGPYRAKINAATMIAQSKNIFQAEIDAACEFIDFLRYNVEFMTQIY
                     ADQPKSTSDVWNRLEYRPLEGFVYAITPFNFTAIAGNLPASAAMMGNVVVWKPSDSQV
                     FSAKIIIDIFKEAGLPDGVINVVFGDALMITDTVLASRDFAGVHFTGSTHVFKDIWAK
                     IGANIHHYKTYPRIVGETGGKDFVLAHPSANVKQVATGIVRGAFEFQGQKCSAASRAY
                     IPKSMWPALKDQLITDVKSMKMGSPEDFGNFITAVIHEGSFDKLASYIDQAKKDADAE
                     IIVGGNYDKSVGYFIEPTIIVTTNPKYTTMETELFGPVITLYVYEDDKWEETLKLVDS
                     TSEYALTGAIFSQNRYAIEEATIALQNAAGNFYINDKPTGAVVGMQPFGGARASGTND
                     KAGSALNLLRWASPRTIKETFVTPEDYRYPFLGE"
     gene            complement(4159..4545)
                     /gene="apaG"
                     /locus_tag="PLQ70_03375"
     CDS             complement(4159..4545)
                     /gene="apaG"
                     /locus_tag="PLQ70_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HQE33686.1"
                     /translation="MVSQITRGIKITVLTSFEGTYFKNYKIHFAFSYEITIENHSKDS
                     VQLISRHWDIFDSLNNAEVVDGEGVIGKKPVLKPGELHTYNSGCLLASPYGAMKGYFN
                     MVNFTSTKTFKVIVPTFRLCAPFALN"
     gene            complement(4628..5881)
                     /locus_tag="PLQ70_03380"
     CDS             complement(4628..5881)
                     /locus_tag="PLQ70_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HQE33687.1"
                     /translation="MFKDFFCKIVLLFVATSTLAQTQTEIIPPYNIKTISFIQSNENV
                     VPIFKLGDGFQFQFDDLFGNEANYYFEIVHCDYNWIPTDIPKTDYMKGFDGQRIQEYE
                     NSVNTLQMYSHYKLPIPNQYMQLRISGNYILKILNESRDVILSRKFIVYEDLVTVPMQ
                     IKRARTANYLDYKHNVEFSIKSQAINFQNPLKNIKVCLMQNGQLNTAIQNIVPQYTIG
                     NDLIYKYDTQTQFWAGNEFLYFDNSDIRSAGNNISRVDSSSGIYNTNLYTNNARANYP
                     YSLTPDVNGNFVVRNIGGTKNEIEADYAWVYFSLSAPSFMKNKGIYITGMFNNYSLSP
                     EYKMDFNKEKNTYEKAILIKQGFTNFQYQIADDKGNIDAENAIDGNFWQTENEYILLV
                     YYRENNDRYERVIGKASANSRDAIN"
     gene            complement(5956..6399)
                     /locus_tag="PLQ70_03385"
     CDS             complement(5956..6399)
                     /locus_tag="PLQ70_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase F system-associated protein"
                     /protein_id="HQE33688.1"
                     /translation="MLQKLLNHKARIALATLFAIGLVVIRAYEDSLFYDPFLNYFKTD
                     YYNLPIPEIDNFKLFLGLFFRYFMNTVLSLAIIYVLFKDIDAIKFASFLYSLFFVILI
                     IALFFILLNTGETNKMGLFYIRRFLIQPLFLLLFLPAFYYQKQKK"
     gene            complement(6380..6931)
                     /gene="xrtF"
                     /locus_tag="PLQ70_03390"
     CDS             complement(6380..6931)
                     /gene="xrtF"
                     /locus_tag="PLQ70_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase family protein XrtF"
                     /protein_id="HQE33689.1"
                     /translation="MKKYFILYKPFLLFLGKFLLTYLLLTFIYQSYLGQFDVKKLELD
                     GFTKTVAQQTKDLMLFFDCDVKSSPNIKEPAVNLFYNQKHIVRIIEGCNALSVIILFV
                     SFIIAFSGKLKSTVLYIIGGSILIHILNVSRIALLCVLIYYYPNQQHLLHGVLFPLFI
                     YGVVFLLWIIWVNKFSKYATKTA"
     gene            7040..7495
                     /locus_tag="PLQ70_03395"
     CDS             7040..7495
                     /locus_tag="PLQ70_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQE33690.1"
                     /translation="MTFEELKPKVTEIISNSTLSREEKLLKICELLSNSIEYYNWVGF
                     YFANHDTKTLHLGPYVGAETDHTVIPFGKGICGQVAVSNSNFVVPDVAAQDNYIACSF
                     TVKSEIVVPLFVNGENIGQIDIDSHVLNPFTEEDERFLEFVNQQVATLF"
     gene            7630..7920
                     /locus_tag="PLQ70_03400"
     CDS             7630..7920
                     /locus_tag="PLQ70_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HQE33691.1"
                     /translation="MSTLSKTDLDAIAINRIIEMAWEDRTPFDAIKFQFGLAEADVKA
                     LMKKELKFSSYKLWRKRVEDCKTKHLAKRIEGIDRFKCNRQRAISNNKITKR"
     gene            7920..8333
                     /locus_tag="PLQ70_03405"
     CDS             7920..8333
                     /locus_tag="PLQ70_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2452 domain-containing protein"
                     /protein_id="HQE33692.1"
                     /translation="MQKEVPDNVVFSKEQGYNAHILPYATNVGAPIIKTDDVVSWKNR
                     GIQNVNKEFESKFNELRLQYQKLMEEFEWNELIYNAKFSFEPVVGEIYHLYIAADSTY
                     FLSIISPQEWNKEHIGSFLLNSDKKWIFINQNTKQ"
     gene            8333..8971
                     /locus_tag="PLQ70_03410"
     CDS             8333..8971
                     /locus_tag="PLQ70_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HQE33693.1"
                     /translation="MKKHFSFEAIMNMEKQERVHFINSLGGFKSVSLLGTVNKQGQTN
                     LGIFSSIFHIGANPSLIALIFRPSPPERDTLRNILETGYYTINHINENIYQQAHQTSA
                     RYDSDISEFDITGLQPEFKNNFKAPFVAESNIQFGVEFKERIDISINNTIMVIGEIKE
                     VYVPENCLGEDGFINLEKANTITCSGLDSYHKTVQLDRLSYAKPDKELTSIA"
     gene            8975..10483
                     /locus_tag="PLQ70_03415"
     CDS             8975..10483
                     /locus_tag="PLQ70_03415"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQE33694.1"
                     /translation="MNKKAINIVWFKRDLRFFDNEALFHAHQSGIPLLLVYFFEPSVM
                     NYDDADLRHWRFIYESIQNLQRKLEATNSKLYFFQKEVATVFTELIKKFDVQTVFSHQ
                     EIGNKLTFDRDIAMQKFFKDNNIVWKEFQLHGVIRKLKSRSDWDKRWENVMRSTPKIL
                     KESDLNLIALDTDFYDTIKGEKLSEAITTPHKNFQQGGEDLAWRYLDSFVKERYINYS
                     KHISKPALSRTGCSRLSPYLAYGNISMRAIYQYTNQFYETAKNKRAVLNFVSRLHWHC
                     HFMQKFEDECRMEFENVNRAYDSLIKPKNETYIKAWQEGKTGVPIVDACMRCLVATGY
                     INFRMRAMVVSFFTFNLWQDWRELHFLARQFLDYEPGIHYPQIQMQSGTTGINTIRIY
                     NPIKNSEEHDSEGIFIKKWIPELAEVPVDLLHEPWKMNEMEQQFYKCKIGLDYPEPIV
                     NIEETRKYASDIVWSFRKKDEVKQEGKRILKKHISNPNKPKNEKRKKTKPAP"
     gene            10449..10589
                     /locus_tag="PLQ70_03420"
     CDS             10449..10589
                     /locus_tag="PLQ70_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010135906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HQE33695.1"
                     /translation="MKSVKKQNLPHKTCLVCQKPFAWRKKWEKVWEEVKYCSDKCRMN
                     KK"
     gene            10686..12221
                     /locus_tag="PLQ70_03425"
     CDS             10686..12221
                     /locus_tag="PLQ70_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HQE33696.1"
                     /translation="MSKTLRLILGDQLNSNHSWFQTTDDSVTYVMMEVRSETDYTSHH
                     IQKIVGFFSAMQNFAKELQSKNHQVIYFHLSDANNFQSFDKNLNYLIEKENFSHFEYQ
                     LPDEYRLDELLKNFCQTLVISYSTVDSEHFMSSRKELGDFFEGKKNFLMESFYHFMRK
                     KHNVLMQGDKPLTGKWNYDDDNRKKLPKNHKPIPPLVFDNNVSEIVTEINKTNIKTIG
                     TIDAKQFVWPTTRAQSLALLDFFVTECLSLFGSYQDAMTPNEWSLYHSRISFSMNVKL
                     ISPLEVINRAIEEWKNRPDEIAYHQLEGFVRQIIGWREYVRGIYWHKMPEFATMNYFD
                     NQEKLPDWFWTGKTKMNCLKDSINQSLNFAYAHHIQRLMITGNFALLAGVHPDEVDAW
                     YLGIYIDAIEWVEITNTRGMSQFADGGIVATKPYVSSAAYIDKMSHYCGSCFYKKAVK
                     TGDKACPFNSLYWNFYDKNEDKLGKNPRIGMMYNVWHKMKPEDKVALLQQADYYLKNI
                     NDL"
     gene            12408..13697
                     /locus_tag="PLQ70_03430"
     CDS             12408..13697
                     /locus_tag="PLQ70_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASH family cryptochrome"
                     /protein_id="HQE33697.1"
                     /translation="MKTTIVWFKTDLRIADNETLTKAITQSEQIVPVYCLDDAHFETT
                     VYGFKKTGSFRAQFLLESLTDLDKNLRALGSGLVVVKGKPEVEIPKLVQQFRAQKVFA
                     KREVAFEEHKTEDLVREALFKLRCELETVSTSTLYHAEGLPFLIKNIPDVFTNFRKKT
                     EQDATIRKPFDSPNKINSPTLPELKLPSLKELGLKKTKIDSRAAIVFKGGETEALKRL
                     KHYFFDTQCLSSYKETRNKMVGTDYSSKFSAWLAMGCISPRFIYAEIKKYESQFGANE
                     STYWLVFELLWRDFFRFMFKKYQTKFFLYEGIKSEKTNSKSLNEKLLSQWINGTTNSD
                     FINANMIELKQTGFMSNRGRQNVASYFCNELNMDWRWGAAYFEEQLIDYDVCSNWGNW
                     AYLAGVGNDPRGHRYFNIEKQASDYDKKKVFRNLWLQ"
BASE COUNT         4727 a         2233 c         2577 g         4345 t
ORIGIN      
        1 gccaaaatca atgtcatttc ccgaaaagat attgatgaat tatacaccaa acacattttg
       61 cattccttgg ggattgcgaa agtcgtaaaa tttgaaccag gaacttacgt tttggatgta
      121 ggaactggtg ggggatttcc gggaattcct ttggcaattc tttttccaga aactcgtttt
      181 tatttgattg atgtcattgc caaaaaaata aaagtggttc aggcggtagt cgatgctttg
      241 gaactcaaaa acgtgaaagc cgaacaaaag agagccgaat tagtaaaagg tgatttcgat
      301 tttatcgtga gtcgtgccgt gaccaatatg cccgattttg tttcttgggt aaaaaccaaa
      361 ataaagaaac aacacaaaca tgaactcaaa aacggaattc tctacctaaa aggtggcgat
      421 ttgaccgaag aattaaaaga tttccccaaa gcaaccgaat ataatttgtc agacttcttt
      481 gaaaatgagt tttttgaaac gaagaaagtg gtgtatttgc cgttgaagtt tcaggcgtaa
      541 ggcaattgaa aactaaataa aaaatgaaaa aatttactga tatatttata acggaattga
      601 acattgaaaa aaaatgtttt gtagacaagt ttagcaatag tatagaaagt tctagaatag
      661 attttatgtc agaatttttg aactctttaa gtttaaaaaa gagtaataaa aaattaattg
      721 gaaaacttaa aaatgattct tttgaactga aaataaattc tactatgaac attttaaact
      781 attcaacagc ggtagctaaa ggtattatta ctgattcaaa aaatggaatt aaaattgaga
      841 ctagaattaa aggctttgat tacagattta ttcctttata tataatttta agcattgtgt
      901 ttattgtatc tctaatagga ctgtttactg attttaaatt tatatttggt ttaataataa
      961 gttctggaat tttcctcttt acaatgaata atatgttgga tgatattcaa aaattaaaaa
     1021 tagaattgac aaacgaaatg aaatcgataa gtgaataaaa atatcttcaa aaacaagatt
     1081 aaagccaaaa acccgcaaga actcaaataa cttgaattct tgtgggtttt gattttttaa
     1141 acgatttttt ttgtaacatt ggtaatatgt aatggactta ctttaaggtt ataatgtaaa
     1201 actaaaaact atgaaaaaaa tttctagacg taaatttgta cataattttg gtcttggtgt
     1261 tggatcaact gctgtaattg catcgcttcc atcatttatg actttgtcag aaaacagtaa
     1321 aaatttgtat gacggtaaaa aactaaatat tgccctttgt ggtttagggc gttatgcggg
     1381 atatttggct gagggtttag aaatttctaa atattgtcgt ttagcaggcg ttgtaacagg
     1441 aacaccgtca aaggctttag attggggaaa aaaatataat attcctgaga aaaatattta
     1501 caattatcag aatttcgatg aaatcataca taataaagac attgatttag tctatgttgt
     1561 tttgccaaat gctatgcaca aagagtttgc aattagagca gcaaaggcag gaaaacacgt
     1621 tattgttgaa aaaccaatgg caattacggc aagcgattgt caagaaatga ttgatgcttg
     1681 taataaagcc ggagtacaat tagcagttgg ttatcgtttg cattatgaac cctataattt
     1741 agaaatgaaa cgattggggc aagaaaaagt gtttggtcag gttcgtttga tagagacttc
     1801 tttagggtac aatacaggtg atacaattga ttggcattcg aagaaagcat tatcaggagg
     1861 aggaccgctt ataaatcttg ggatttattg tgttcaggca agtcgttata ttttgggtga
     1921 agagccaatt tcagtaacag cacaatttgg accagttacc aataaaataa aatttgcaga
     1981 agtagaagaa tcaattactt ggcaattgaa ttttcctagc ggagcagttt gtaattctgc
     2041 aagttcatat aattgtggag ttgacagatt atttgcttca gcagaagacg gtttttttga
     2101 gctaagtcca gctataagtt atggcccatt taaaggaagg acaagcaatg gagatttaaa
     2161 ttttcctgaa attaatcaac aacaaacaca acttgatgaa ataggaaaat tgattttaga
     2221 aaataaaaaa ttaccaagtc atattacagg cgaagaagga cttaaagacg ttacaatttt
     2281 acaagctatt tatgaggcag caaggacagg aaaaaaagtc tcattggttt aaaaatcaaa
     2341 acccacaaga actcaattac ttgattcttg tgggttttta aatttcgtac ctcgtatttc
     2401 taacttcgta ccttcaaaaa ttattctccc aaaaacggat atctgtaatc ttctggagtt
     2461 acaaaagttt ctttgatggt tctaggcgaa gcccaacgca acaagtttaa ggcagaaccc
     2521 gctttgtcat tcgttccaga agctcttgct ccaccaaaag gttgcattcc cacaacggct
     2581 ccagttggtt tgtcgttgat gtagaaattt cctgctgcgt tttgcaaagc gatggttgct
     2641 tcttcaatcg cataacgatt ttggctgaaa atagctccag tcaaagcata ttctgaagta
     2701 gaatcaacca gttttagagt ttcttcccat ttgtcgtctt catatacata aagggttatt
     2761 accggtccga ataattcggt ttccattgtg gtgtattttg gattggttgt tacgattatc
     2821 gtcggttcaa taaaatatcc cacggattta tcatagtttc caccaacaat aatttcggca
     2881 tcggcatctt ttttagcttg gtcaatataa ctcgctaatt tgtcgaaaga accctcgtgg
     2941 attactgcgg taatgaagtt gccaaaatct tctggcgatc ccattttcat tgattttaca
     3001 tcagtaatca attggtcttt aagggctggc cacatacttt ttggaatata ggctcttgaa
     3061 gctgctgaac atttttgtcc ttgaaattcg aatgctccac gaacaattcc tgtggcaact
     3121 tgtttcacat tagcacttgg atgcgctaga acaaaatctt ttccaccagt ttcgcctacg
     3181 attcttgggt aggttttata atggtgaatg ttggctccaa tttttgccca aatatctttg
     3241 aacacgtgag tggaacctgt gaaatgtact ccagcaaaat cgcgacttgc caaaacggta
     3301 tcggtaatca tcaaggcatc accaaaaacc acgttgataa cgccatcagg aagtcctgct
     3361 tctttgaaaa tatcgataat gattttagca gaaaatactt gactgtcgct gggtttccaa
     3421 actactacat ttcccatcat agcggcactt gctggaagat ttcctgcaat ggctgtaaag
     3481 ttgaaaggcg taatagcata aacaaaacct tctagcggac ggtattccaa tctgttccaa
     3541 acatcagaag ttgatttggg ttggtcggca taaatttgag tcataaattc aacgttgtaa
     3601 cgcaagaaat ctatgaattc acaagctgca tcaatttcgg cttgaaagat atttttggat
     3661 tgcgcaatca ttgttgcggc gtttattttg gctctgtaag gaccagcaat caattcggca
     3721 gcttttagaa aaatggctgc gcgttgttcc caagccatat tcgcccattt ggttttggct
     3781 tcaagggcat tggcaatcgc tttttcgata tgtgattttt cggcaagatg ataggttcca
     3841 actacgtgtt tatgatcgtg aggtgcggtc atatttcgag tattgccagt tctaatttct
     3901 tcacttccaa tgtaaagtgg aacatcgatt ttggagtccc acattttttt ataagcagct
     3961 agaacggcag ctttttctgg ggaattgggt gcatacgatt ttacaggttc gtttacggct
     4021 tttggtacat tgaaaaatcc tttaggcata tgttttttat ttgagaatta gaaaattttc
     4081 acaaaagtac aaaggattgt ttgaaaaata taaaatttaa ctaaaagata atagatgtga
     4141 ttaattgaaa ttgaactatt agtttaaggc aaaaggagca cacaaacgga atgttggcac
     4201 aattactttg aaggtttttg ttgaggtgaa attaaccata ttgaaatacc ctttcattgc
     4261 gccatatggt gaagctagta ggcaaccaga gttataagta tgtaattctc ctggttttaa
     4321 aactggtttt ttcccaatta cgccttcgcc atcaactact tcggcattat ttagggaatc
     4381 aaaaatgtcc caatggcgtg atattaattg aacggaatct ttgctgtggt tttcgatggt
     4441 gatttcataa ctaaaggcaa aatggatttt gtagtttttg aagtaagtgc cttcaaaact
     4501 agtcaaaacg gttattttta tacctcttgt aatttgagaa accatagata gcaaagtgtt
     4561 acaaatgagt ttgataatcg caaatttaca aaaaaaagag ttaattgtgc ttttataaga
     4621 aattttttta attgatggca tctctagaat tggctgaggc ttttccgata actctttcgt
     4681 atctatcgtt gttttctcga taataaacca gtaggatata ttcattttcg gtttgccaaa
     4741 aattaccatc tatagcattt tctgcgtcga tatttccttt gtcgtcggcg atttgatact
     4801 gaaaattagt aaatccttgt ttgatgagaa tggctttttc gtaagtgttt ttttctttgt
     4861 tgaaatccat tttgtactct ggcgacaggc tgtaattatt gaacattcca gtaatataaa
     4921 tgcctttgtt tttcataaaa ctaggcgccg atagactgaa ataaacccaa gcataatcag
     4981 cttcaatctc gtttttggtg ccaccaatgt tgcgaaccac aaaatttcca ttcacgtctg
     5041 gagttaaaga ataagggtaa tttgctctgg cattattcgt gtataaattt gtattataaa
     5101 ttccgctgct tgaatctact cgagaaatgt tattgccagc agatcggatg tcgctattgt
     5161 caaaatacaa aaactcgttg ccagcccaaa attgagtttg tgtgtcatat ttataaatta
     5221 aatcattgcc aatggtgtat tgcgggacta tgttttggat ggcagtattc aattgtccgt
     5281 tttgcattag gcaaactttt atgtttttca aggggttttg aaaattaata gcttgcgact
     5341 tgattgaaaa ttcaacattg tgtttgtaat ccaaataatt ggctgttcga gctcttttaa
     5401 tttgcatcgg aacggttaca agatcttcat aaacgatgaa ttttcgagat aaaataacat
     5461 ctcggctttc gtttaagatt ttcaagatat aattgccact tatgcgcaat tgcatatact
     5521 gattgggaat gggtaatttg taatgcgaat acatttgtaa agtattaacg gagttttcgt
     5581 attcctgaat tctttgtcca tcgaaacctt tcatgtaatc ggtttttgga atgtctgtgg
     5641 gaatccaatt gtaatcacaa tgcacaattt cgaagtaata attggcttcg tttccgaata
     5701 aatcatcaaa ttgaaattgg aatccatcac ctaatttgaa tattggaact acattttcgt
     5761 tgctttgtat gaatgatatc gttttgatat tataaggtgg aataatctcg gtttgggttt
     5821 gtgcaagtgt cgaagtagct acgaaaagca atactatttt acaaaaaaaa tctttaaaca
     5881 tagctatgta attggtgatt ggtaaatata acgaaatata tttcaatatt atttgacgtt
     5941 tagacactca aaggcctatt tcttttgttt ttgataataa aaagcaggaa gaaataacaa
     6001 caaaaatagc ggctgaatta ggaatcgtcg aatgtaaaac aagcccattt tgttcgtttc
     6061 tccagtattt aaaagaataa aaaagagagc aataatgagg attacaaaaa acagagaata
     6121 caaaaacgaa gcaaatttta tagcgtcaat atctttaaat agaacataaa ttatggctaa
     6181 tgacagcaca gtattcataa aatatctgaa aaataaaccc aaaaacaatt tgaaattgtc
     6241 tatttctgga atgggcaaat tgtaataatc agttttgaaa taattcaaaa aagggtcgta
     6301 aaacaagctg tcttcgtagg ctcgtattac aaccaatcca attgcaaaaa gcgttgccaa
     6361 tgcaattctc gctttgtgat taagcagttt ttgtagcata ttttgaaaat ttattgaccc
     6421 aaataatcca taacaaaaaa acgactccgt aaataaataa aggaaataaa actccgtgca
     6481 aaagatgttg ctggtttgga taataataga tcaaaacaca caaaagcgca attctagaaa
     6541 cgttcagaat gtgaatgaga atgcttccgc caatgatgta cagaacagtc gattttagtt
     6601 ttccagaaaa ggcaatgata aatgaaacaa acaagatgat tacgctcaat gcgttgcaac
     6661 cttcaataat tcgaacgata tgcttttgat tgtagaacaa attcactgcg ggttctttga
     6721 tgttgggtga ggattttaca tcacaatcaa agaaaagcat taggtcttta gtttgttggg
     6781 ctactgtttt agtaaatcca tctagttcta atttcttgac atcaaattga ccaagatagc
     6841 tttggtagat gaaggtcaac agtaaatagg ttagtaaaaa cttgcctaaa aacagcagaa
     6901 agggtttgta gagaataaaa tattttttca aaataaaatt tttaacaaat ttatataaaa
     6961 atagaagaca attttcactt caaactttct acttcaaact tcccactttt ttaaatactt
     7021 ttacaaaaaa taactgaata tgactttcga agaattaaaa ccaaaagtaa ccgaaattat
     7081 ctcaaattcc actctttcaa gagaagaaaa actattgaaa atttgtgaat tgctgagtaa
     7141 ttctattgaa tattacaatt gggttggatt ttacttcgcc aatcacgaca cgaaaactct
     7201 gcatcttggt ccgtatgttg gcgcagaaac cgatcatacc gttatccctt ttgggaaagg
     7261 catttgtgga caagtggcgg tttctaactc taattttgta gttcctgatg tcgctgcgca
     7321 agacaattat atagcctgca gttttaccgt aaagtccgaa attgttgtgc cactttttgt
     7381 aaatggcgaa aatattggac aaatagacat cgatagtcat gtgctgaatc cctttaccga
     7441 agaagatgaa agatttttgg aatttgtgaa ccagcaagtg gcgactcttt tttaataaaa
     7501 ctactccatt atttatttaa tccgttgctt aaatttaagc aacggatttt ttatttgtaa
     7561 ttataatggg atttgaaatt taattattta attttgttta actttattta attttaatta
     7621 aacaaaataa tatcaacatt atcaaaaaca gatttagatg ctattgcaat taatcgaatt
     7681 atcgaaatgg cttgggaaga ccgcacccca tttgatgcca ttaaatttca attcggattg
     7741 gcagaagccg atgtgaaagc cttgatgaaa aaagaactca aattttcgag ttacaaattg
     7801 tggcgaaaac gtgttgaaga ttgcaaaacc aagcatctag ccaagcgtat cgaaggcatt
     7861 gaccgattta agtgcaacag acaaagagct atttcaaaca acaaaataac caaacgatga
     7921 tacaaaaaga agttccagac aatgttgttt tctcgaaaga acaaggatat aacgcccata
     7981 ttttgcctta tgctaccaat gttggcgcac caattatcaa aacagatgat gttgtttctt
     8041 ggaaaaatag aggcattcaa aatgtaaaca aagagtttga aagcaaattc aacgaattga
     8101 ggcttcaata tcagaaatta atggaagaat ttgaatggaa tgaactgatt tacaatgcaa
     8161 aattctcgtt tgaacccgtt gttggtgaaa tatatcatct ttatatcgct gccgacagca
     8221 cttatttttt gtctatcatt tctccgcaag aatggaacaa agaacatatt ggttccttct
     8281 tattgaatag tgacaaaaaa tggattttta tcaatcaaaa tacaaaacaa taatgaaaaa
     8341 acacttttct tttgaggcaa taatgaatat ggaaaaacaa gaacgtgttc attttattaa
     8401 tagtttgggc ggattcaaga gtgtttcctt gctaggaact gtcaataaac aagggcaaac
     8461 caacttgggg atttttagtt caattttcca tattggggct aatccttcgt tgattgcttt
     8521 gatatttaga ccaagtcctc cagagcgaga tacgttacgc aacatcttag aaacgggtta
     8581 ttacaccatc aatcacatta acgaaaatat ataccaacaa gcacatcaaa cttcggcacg
     8641 atacgactcg gatatttctg agtttgacat aacgggtttg caaccagagt tcaaaaacaa
     8701 ttttaaagcc ccatttgttg ccgaaagtaa cattcaattt ggagttgaat tcaaagagcg
     8761 aatcgatatt agcatcaaca ataccataat ggttattggc gaaattaaag aagtttatgt
     8821 tcctgaaaac tgccttggtg aagatggttt tatcaacctc gaaaaagcga ataccattac
     8881 ttgttcggga ttagatagct atcataaaac cgtgcaatta gaccgattga gttatgctaa
     8941 acccgacaaa gaattaactt ctattgccta aaaattgaac aaaaaagcca tcaatattgt
     9001 ttggttcaaa cgcgacctcc gtttctttga taacgaagca ttgtttcacg cccatcaatc
     9061 gggaatacct ttgttattgg tgtatttttt cgaaccatcg gttatgaatt atgacgatgc
     9121 cgatctgagg cattggcgtt ttatttacga atcgattcaa aatttgcaac gaaaattaga
     9181 agcaacaaac tctaaattat atttttttca aaaggaagtt gctactgttt ttactgaatt
     9241 aattaaaaaa ttcgacgttc aaacggtttt ttcacatcaa gaaattggca ataaattgac
     9301 ttttgacaga gatattgcaa tgcaaaaatt ctttaaagac aataacatcg tttggaaaga
     9361 atttcaactc cacggtgtaa ttcgaaaatt aaaatcgaga agcgactggg acaaacgctg
     9421 ggaaaacgta atgcgatcca caccaaaaat cctaaaagaa tccgatttga acttaattgc
     9481 tttagacacg gatttttatg atactataaa aggagaaaaa ctatccgaag caattacaac
     9541 tccccataaa aatttccaac agggtggcga agatttggct tggaggtatt tggatagttt
     9601 tgtcaaagaa cgttatatca attacagcaa acacatctca aaacctgcct tgagccgaac
     9661 aggttgtagc cgattgtctc cttatttagc gtatggaaat atcagtatgc gagctattta
     9721 tcaatatacg aatcagtttt atgaaaccgc caaaaacaaa cgagcggtac tgaattttgt
     9781 ttcccgtttg cactggcatt gtcattttat gcaaaaattt gaagacgaat gccgaatgga
     9841 atttgaaaac gtcaatcggg cgtatgacag cttgataaaa cccaaaaacg aaacctatat
     9901 caaagcttgg caagaaggca aaacaggcgt tcccatagtg gatgcgtgta tgcgatgctt
     9961 ggtggcaacg ggctatatca acttcagaat gcgtgcgatg gtcgtttctt tttttacctt
    10021 caatttatgg caagattggc gggaattgca ttttttagca cgccaatttt tagactacga
    10081 acccggaatt cattatccgc aaattcagat gcaatcgggt acaacaggta tcaataccat
    10141 tcgaatatac aatcccatta aaaactctga agagcacgat tcggaaggga tttttatcaa
    10201 aaaatggatt cctgaattag ccgaagttcc cgttgatttg ttacacgaac cttggaaaat
    10261 gaacgaaatg gagcaacaat tttacaaatg caaaatcgga ttggattatc cagaacctat
    10321 tgtaaatatt gaagaaactc gaaaatatgc cagcgacata gtgtggagtt ttcgcaaaaa
    10381 agacgaggta aaacaagaag gcaaacggat tttgaagaaa cacatttcaa accccaataa
    10441 acctaaaaat gaaaagcgta aaaaaacaaa acctgcccca taaaacctgt ttggtgtgtc
    10501 aaaaaccttt tgcttggcgc aaaaaatggg aaaaagtttg ggaggaagtg aaatattgta
    10561 gcgataaatg ccgaatgaat aaaaaataat ttagccacag attaaaatga ttttcacaga
    10621 tttttttgct gtgagatttt taaaattctt ttaatctttt gaatctgtgg caaaaaaata
    10681 acaaaatgtc aaaaaccctc aggttaattc tcggcgatca attgaacagc aatcattctt
    10741 ggtttcaaac tacggatgat tcggtgactt atgtaatgat ggaagttaga tccgaaactg
    10801 attatacctc acatcatatt caaaaaattg ttggtttttt ttcggcgatg caaaattttg
    10861 ccaaagaatt acaatcaaaa aatcatcagg taatatattt tcatttgagt gatgcaaata
    10921 actttcaatc tttcgataaa aacctaaact atcttattga aaaagaaaat ttcagccatt
    10981 tcgaatacca attgcccgat gaataccgac tggatgaatt gttgaaaaac ttttgtcaaa
    11041 cgcttgtcat ctcctattcg actgtcgatt ctgaacattt tatgagttcg agaaaggagt
    11101 tgggcgattt tttcgaaggc aaaaaaaact ttttaatgga aagtttctat cattttatgc
    11161 gaaaaaagca caatgtatta atgcaaggcg acaaaccttt gactggaaaa tggaattatg
    11221 atgacgacaa tcgcaaaaaa ttacccaaga atcacaaacc aattccgccg ttagttttcg
    11281 ataataacgt ttctgaaatt gtaacggaaa tcaataaaac caatatcaaa accattggaa
    11341 ctattgatgc aaaacaattc gtttggccaa caacaagagc acaatcttta gcattattgg
    11401 atttttttgt caccgagtgt ttgtcgcttt ttggcagtta tcaagatgcg atgacgccta
    11461 acgaatggtc gttgtatcat tctcgaattt cgttttcgat gaatgtaaaa ctgatttcac
    11521 cgttggaagt catcaacaga gccatcgaag aatggaaaaa tcgccctgat gaaattgcct
    11581 atcatcaatt ggaaggtttt gtgcgtcaaa ttattggctg gcgagaatat gtgcgaggca
    11641 tttattggca caaaatgccc gaatttgcta cgatgaatta ttttgacaac caagaaaaac
    11701 tacccgattg gttctggact ggaaaaacca aaatgaattg tctgaaagat tccattaatc
    11761 aatctttgaa ttttgcttac gcacatcata ttcaacgttt gatgattaca gggaattttg
    11821 cgcttttggc aggagttcat cccgacgagg ttgatgcttg gtatttgggc atttatattg
    11881 atgccatcga atgggtcgaa attaccaata cccgaggaat gagtcagttt gccgatggtg
    11941 gcattgtagc tacaaaaccc tatgtgagtt cggcggccta tatcgataaa atgagtcatt
    12001 attgcggttc ttgtttttat aaaaaagccg tcaaaacagg cgataaagcc tgtcctttca
    12061 acagtttgta ttggaatttt tatgataaaa acgaagataa attgggcaaa aatccccgca
    12121 ttggaatgat gtacaatgtt tggcacaaaa tgaaacccga agataaagtc gcattattac
    12181 aacaagccga ttattatttg aaaaacataa atgatttata aaaaaatagt ttcaagttta
    12241 aggtttcaag ttgtggaaca ttaaacggcc tctgggttta gccactaagg agttagcaaa
    12301 ttcaaattac acatatttat ataaataaat ctgtggaaat ctgtggcaaa aaaacaaatc
    12361 gaatcaaaac ttcgaggaat taaacttgaa gcctgaaact caaaaaaatg aaaacaacta
    12421 tagtttggtt caaaactgat ttaagaatag ccgataacga aaccctgact aaggccataa
    12481 cacaaagcga gcaaattgtt ccggtgtatt gtttggacga tgctcatttt gaaactacgg
    12541 tttatggatt caagaaaaca ggttctttta gagcacaatt tttactcgaa tcgttaaccg
    12601 atttagataa aaacctaaga gctttggggt cgggattggt ggttgtaaaa ggcaaacccg
    12661 aagttgaaat tcctaaattg gttcaacaat ttagagcgca aaaagttttt gccaaacgag
    12721 aagtcgcttt tgaagagcat aaaaccgagg atttggttcg tgaagccttg ttcaaattgc
    12781 gatgcgaatt agaaacggtt agcacgagca ccctttatca cgccgaaggc ttgccgtttt
    12841 taataaaaaa tattcccgat gtttttacta attttagaaa gaaaaccgaa caagatgcta
    12901 cgattcgaaa accatttgac agcccaaata aaatcaactc accaacactg cctgaattga
    12961 aattacctag tttaaaggag ttgggtttaa agaaaaccaa aatcgattca agagccgcaa
    13021 tagttttcaa aggtggtgaa acagaggcgt taaaaagact aaaacattat ttttttgaca
    13081 cccaatgctt gtctagttac aaagaaaccc gaaacaaaat ggtgggtacc gattattcgt
    13141 ctaaattttc ggcttggctg gctatgggct gtatttcgcc tcgattcatt tatgctgaaa
    13201 ttaaaaaata cgaatcacaa tttggtgcca acgaatctac ttattggttg gttttcgaat
    13261 tgttgtggag ggattttttc agatttatgt tcaaaaaata ccaaaccaaa ttctttttgt
    13321 atgaaggtat caaatccgaa aaaacaaact caaaatcctt gaacgaaaag ttgctttctc
    13381 aatggataaa cggaaccact aactctgatt ttatcaatgc caatatgatc gaattaaagc
    13441 aaactggttt tatgagtaac cgtggacgtc aaaatgtggc gagttatttc tgtaatgaat
    13501 tgaatatgga ttggcgttgg ggagccgctt attttgaaga acaattgatt gattatgatg
    13561 tgtgcagcaa ttggggcaat tgggcttatt tggctggagt gggcaatgat cctcgtggac
    13621 atcggtattt taatatcgaa aaacaagcga gtgattatga taaaaaaaag gtttttagaa
    13681 atttgtggtt gcaataaatc acaactataa gtgctaaaat taatgctttt ttacaattaa
    13741 cccgttttgt tagccatttt ataaaatact tgatgtataa aaaacctatc acttagaaag
    13801 ttttttatat caataggaga agatttacag ccacgtccta aattctttta ccgtagaaga
    13861 ggaaagattt ttggaatttg tg
//
LOCUS       DAOSNN010000038        13858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_4445_length_13858_cov_5.800840, whole genome shotgun sequence.
ACCESSION   DAOSNN010000038 DAOSNN010000000
VERSION     DAOSNN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 13858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13858
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4445_length_13858_cov_5.800840"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLQ70_03435"
     CDS             <1..342
                     /locus_tag="PLQ70_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HQE33698.1"
                     /translation="RCDVRWMALTTPQKNKGFAIVNDTKVLSMSAWPYTQENLSAATH
                     TYDLKDPGFLTVNIDLVQMGVGGNDSWTLVAQPLEQYQIKSGNYQYSFYLVPFMGSKN
                     ELESSLKKFKY"
     gene            375..3767
                     /locus_tag="PLQ70_03440"
     CDS             375..3767
                     /locus_tag="PLQ70_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQE33699.1"
                     /translation="MLLLAGFASAQENPKKENSLFQPTLESSRPWVYWYWMQSAYSKA
                     GITADLEAMKQAGIGGAYLMTIKGPANPPLMDPPVLQLSPEFWNLVHWALTEADRLGV
                     KIAFHPADGFAVAGGPWITPEMSMQKVVWADTFVNGGSKIQTKLAVPNHYKDYYQDIA
                     VFAIPVKESCITSNQNRPKITSTIADFDASFLSDSNKDDQFRLKEKGWIQYEFDKPFL
                     CKSIQIETKGNNYQAHRLLIEVSDDGVNFKSLGRLTTPRHGWQDDDAFYTHSIVPIKA
                     KYFRFVYNPESSEPGAEDLDPAKWNQGLKVSKMLLSNESLIDNYQGKSGAVWRVSPQT
                     TEAQIPNSDCVSKSGIINISKFVDANGTLSWLSPKGKWRIIRMGHTSTGHENATAGAA
                     RGLEVDKFNPEAVRFQLDHWFGEMLRIAGPELASKVVKILHIDSWECGSQNWSPVFQA
                     EFKNRRGYDLVDYLPVMAGIPIESAATSEKVLYDVRKTIAELVADNFFGTLANIAQKA
                     NVKFSSENVAPTMMSDGLLHFKHIDYPSGEFWLKSPTHDKPNDMLDAISGGHIYGKDI
                     IQAEAFTELKMDWDEHPGNLKTLADRNYAIGINRFFYHVFAHNPWTDRKPGMTLDGVG
                     SYFQRDQTWWKPGKAWVDYCQRVQFQLQKGKPVIDLAVFIGEDLPSRSVLPDRLVPFI
                     PNIFGKERLESEAIRLKNEGQPSIKMPKEVTSTKNSTDLSQWVNAMNGYQYDSFNADV
                     LLNNAKVENGKVIFSNSIQYGALLFPGSHKMAPNKMISLAVAKKILELVKDGATVFID
                     EKPNLQPGIYSEADHKNWQTTIDEIWNNTSNATSWKIGQGTIIELPYLGNDFAEIGIT
                     QDVYFPKLNRADSETLAWTHRRSDEEDVYFLSNQKAEKRQFEASFRVVGKIPVWYNPV
                     TDKTSALANWKIENGRTIVALALDANESGFVIFKEETKEVQAKGYQKGLEFENVQTLD
                     ENWELQFDSEYKGPKEVVKTNKLFDWSTSENNQIKYYSGTVIYKKDFVWNGKDTNNLW
                     LDLGTIANMAEVSINGKDCGTLWTFPYKVDVSNALKKGKNTIIIKITNTWANRLIGDQ
                     KLPKEERLTWTTAPFRLEGNPLLKAGLLGPVTIVKEKSKRNE"
     gene            3760..5193
                     /locus_tag="PLQ70_03445"
     CDS             3760..5193
                     /locus_tag="PLQ70_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQE33700.1"
                     /translation="MNKFRNHIIAIVLLLTSFQINAKIKLPALFSDNMMLQQKSNAPI
                     WGWAEKNANITIKTSWNAKIYKVKADNSGKWKTELQTPSFGGPFTIEVSEGAEKVVIK
                     NVLVGEVWLCSGQSNMEMPLKGFSGQPVRNGNEIIVKSTNATIRLITIPRATVLQPLD
                     NFEGKWEVASPKSTANFSATAWYFGSLLQEVLQVPVGLIHVSYGGSSMEAWMNQEMLK
                     DFASAKIPTTKEELAKDPNRVPTTLFNGMLSPVIGYGIKGCIWYQGESNYERASEYTV
                     LMKKMVSSWRAMWKQGDFPFYFCQIAPFNYASFHPKDYLEKYNSAYLREAQLKASKEI
                     PNTGLAVLMDVGEENNIHPTDKEKGGNRLAFQALSKTYGIEGFEFESPKYKAMEIKDN
                     SVTVSFDDVPNGITSYGKEVNGFEIAGEDKVFYPAKAEVRRKSVVLTSDKVAKPVAVR
                     YLWKDFVKAELFSNGGLPMSSFRTDEW"
     gene            5292..7619
                     /locus_tag="PLQ70_03450"
     CDS             5292..7619
                     /locus_tag="PLQ70_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 domain-containing protein"
                     /protein_id="HQE33701.1"
                     /translation="MKYIKYILLFTTFLVKAQIPVLDNYNQIWTTQSAYSFESMPLGG
                     GDIGLNVWVEKGDLYFYFSRSGTFDEHNTLLKLGRVKVSLSPNPFTGEEGFRQELKLK
                     DGCILIGQNDVKIKLWVDVFKPIIHLDLESENPLKMTASYESWRYKNLDSKGKANNAN
                     SYKWAPQGDIVTYKDSIAFENNGIKFYHRNREQTVFDVAVKQQKMESVKDQMMNPIAN
                     LTFGGFMVGENLKPDGTYLGKYQETDCKGFNLSSIKPSKKHSLEIYLNTSQSDFSTWN
                     NGLKNQISEYKTKGKQAEKNTQIWWNNFWKRSFIFTQKNQSTAKDSVYQIGQNYQLFR
                     YMLGCNAYGKYPTKFNGGLFTVDPVFTNPDLNFTPDFRNWGGGTMTAQNQRLVYYPMT
                     KSGDFDMMKSQLDYYVSLQKNAELRSKVYWKHGGAAFTEQLENFGLPNPAEYEWKRPT
                     DYDRGMEYNAWLEYEWDTVFEFCKMMLEQKEYANEDIQKYNPFIVSCLRFFDEHYQYL
                     AKQRGRKALDGDGHLILFPGSGAETYKMANNANSTISALTVITEKILTLSDKELSKED
                     VDYLKGFQTRIPPLNFREFDGNKTLAPAKSWERINNSEVPQLYSVFPWGIYGIGKLDL
                     ETALNTWKYDTDAIKFRSHVGWKQDNIFAARLGLTKEAAKYNLLKMANSERRFPAFWG
                     PGYDWVPDHNWGGSGMIGMQEMLLQEANGKIYLFPAWPKDWNVHFKLHATQNTTIEAE
                     LINGELKILKVIPEERKKDVINLLDKSEAEKIKLN"
     gene            7621..10068
                     /locus_tag="PLQ70_03455"
     CDS             7621..10068
                     /locus_tag="PLQ70_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HQE33702.1"
                     /translation="MIKKMMGAAALAIILATNGQAQNTEKQIVKVDFDFSQRRIDEVN
                     DVTYSSWPISEQKQDEKSFNGLTFKLKGNFNSKWYKVGMNAPFYSKLASDGLATAENL
                     ELTISGLKAGQHSLLTFHNTFDKIEGKTFSPVKIYVNDKLAETVLQSNRSNSKIETAT
                     AYILFNAEANKDVVIRFELDAKATDLIQQMIINGFEIDTPNLKKQSHTPKPENANEHV
                     VIDKNLVLNWESAKDAVAHQIYFGTDSNAVSNANDKSPEFKGKLTENKFDLGKLYSGT
                     TYYWRVDELDAKGIVTLGTVWSFKPAQLAFPEAEGYGRFAVGGRGGKVVEVTNLNDSG
                     PGSLRDAVDQQIGRKTIVFNVSGNIKLASRLVVNQPYVTIAGQTAPGEGITITRAPIG
                     LTGNDGVVRFLKVRIGGGTTYDGMGLTGANHSIIDHCSISWTIDESFSSRGAHNITLQ
                     RTLISEALNVAGHDKYPAGKMHGYAATIGGDIGSFHHNLLAHNEGRNWSIGGGLNGDG
                     YYGGKIDIRNNVVYNWGHRATDGGANQVNFVNNFYKPGASTKIFVALNAQIEGVGKGK
                     QQYYFDGNTMPGYFNEKNQEKGRKFTLSNKAIVDYEVFLNKPFFESYVNTQTADAAYK
                     NVLSDVGANAPFFDKHDNRIIDETLKGTYTYKGSKSGLGGLIDNEADQGGWPNFATEN
                     RPADWDTDHDGLPNWWEKAKRLNENSKNGDFSDAGLDADKDGFTQLDDYLDWMAQPHF
                     FVKTGEKLTLDAAIYFKGYEKKPVYTFSEVKNGKAVLKGSTIKFTAESKDFASFKITV
                     TDKDGDTMTRTINVFVK"
     gene            10164..12182
                     /locus_tag="PLQ70_03460"
     CDS             10164..12182
                     /locus_tag="PLQ70_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HQE33703.1"
                     /translation="MKKLILTALLFSNAFVFSQNKGLVANSDSPYSKLQSVAMQDVKW
                     TNGFWKEQFDVETKNTLPYMWDLYHNDEVSHAYANFKIAAGESKGTFKGPSFHDGDFY
                     KIFEGMAATYAVTKDKKLDAEMDKAIALFAKVQRKDGYLHTPVLIDERWGTLGPEEVK
                     KQLGFEKYNMGHLMTAACIHYRATGKTNFLTIAKGVADFLYDFYKKASPELARNAICP
                     SHYMGIVEMYRTTKNPKYLELANNLIDIRGTTNDGTDDNQDRIPFRQQTTAMGHAVRA
                     NYLYAGVADLYAETGEKKLLDNLESIWDDVTYRKMYITGACGSLYDGVSPDGTSYNPT
                     DVQKIHQAYGRPFQLPNATAHTETCANIGNVLWNWRMLQITGDAKYADIVELALYNSV
                     LSGISLEGKEFFYNNPLNVSKNLPFKQRWSKEREGYIALSNCCAPNVTRTIAEVNNYA
                     YSLSKEGLYVNLYGSNNLNSTTLNGEKIEIEQQTNYPWDGKVSLKIVKAPKDIYAFFL
                     RIPGWSQGTTISINGKNINDAIASGSYQKIAQKWKKGDVIELNIPMPVELMQANPLVE
                     EVKNQVAVKRGPIVYCLESNELPANTKVNDVILDVNSKFTTDFIKIDNRQLLSITASS
                     SIIPDNSWDKKLYKPISIKDNKEYTVKLIPYFAWGNQGKGEMSVWLSH"
     gene            12383..13291
                     /locus_tag="PLQ70_03465"
     CDS             12383..13291
                     /locus_tag="PLQ70_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQE33704.1"
                     /translation="MKKTITFLLLLTIVQIQAQEFMPLWTKGKMPNSKGLVVKDSINN
                     ERVMQVGMPGIHVFFTSIQENKGAAVLIIPGGGYHHLSYNISGFQLAKWFNTMGINAF
                     VLTHRLPISPDLKEREKAPLQDAERAMRIIRKNAVNWKIDTNKIGVMGCSAGGHLAAS
                     LGTIKDDFAKIGDDFDSFSFRPDFMILVSPVIDMGKETHKGSLENLLGKNPSSELINK
                     YSLQFQVSNYTPPTFITHAFNDKVVSPKNSLLFYNALLENKIPSSLHIFNQGAHAIAL
                     RNNPGSTNIWTAICEQWLMEINILKN"
     gene            13318..>13858
                     /locus_tag="PLQ70_03470"
     CDS             13318..>13858
                     /locus_tag="PLQ70_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HQE33705.1"
                     /translation="MKHFLTTLLFLTTFFLTAQTTDNLFALTVAQDGSGDFKTIQEAI
                     NKVRDHAEMRVVITIKAGTYNEKVVIPSFKRNITLRGTDREKTIISYDDYSGKPFRGI
                     DVTGNEKYSTYTSYTLLVQGNDCSVENLTIQNTAGRVGQAVALHTEGDRVAVKNCSIL
                     GNQDTLYLAKGGTRNYFENC"
BASE COUNT         4587 a         2396 c         3083 g         3792 t
ORIGIN      
        1 cgatgtgatg tgagatggat ggcacttaca actccgcaaa aaaacaaggg ttttgcaatt
       61 gtgaacgata caaaagtgtt gagtatgagt gcttggcctt acactcagga aaatttgagc
      121 gccgcaaccc atacttatga tttaaaagat cctggatttt tgacggtaaa tattgatttg
      181 gttcaaatgg gtgttggagg aaacgacagc tggactttgg tagcacaacc tttggaacaa
      241 tatcaaatta agtctggaaa ttatcaatac agtttttatt tggttccttt tatgggttca
      301 aaaaatgaat tggaaagcag tctgaaaaag tttaaatatt agttccaatg ttcaacaaaa
      361 aacacttctt ttttatgtta ctccttgcgg gatttgcttc cgcacaggaa aatcctaaaa
      421 aagaaaacag cttgtttcaa cctacattag aatcctcaag accttgggtg tattggtatt
      481 ggatgcagag cgcctattct aaagcaggca tcaccgctga tttggaagcg atgaaacaag
      541 ccggaattgg aggtgcttat ttgatgacga ttaaaggccc ggcaaatccg ccgttgatgg
      601 atccgccagt gttgcaatta agcccggaat tctggaattt ggtgcattgg gcgttgaccg
      661 aggcagaccg attgggcgtg aaaattgcgt ttcatcccgc cgatggtttt gccgttgccg
      721 gagggccttg gattacaccc gaaatgtcga tgcaaaaagt cgtttgggca gacactttcg
      781 tgaatggagg atccaagatt caaacgaaat tagcggttcc aaatcattac aaagactatt
      841 atcaagatat cgccgtgttt gccattccgg taaaggaaag ttgcatcaca tccaatcaga
      901 atcgtcccaa aattacttct acaatagccg attttgacgc ttccttttta agcgattcca
      961 ataaggacga tcaatttaga ttaaaggaaa aaggatggat tcaatatgaa tttgacaagc
     1021 cgtttttatg caaatcgatt caaatagaaa ccaagggaaa taattatcag gcacatcgtt
     1081 tactaattga agtcagtgac gatggtgtca atttcaaaag tttgggtcga ttaacaacgc
     1141 cacgccacgg ttggcaagat gatgatgcgt tttacacaca cagtatcgtt cccataaaag
     1201 ccaaatattt ccgatttgtt tacaatcccg aaagctcaga accgggcgcg gaagatttgg
     1261 atccagccaa atggaaccaa ggattgaaag tgtcaaaaat gcttttgtcc aacgaatcct
     1321 tgattgataa ttatcaagga aaatcgggtg ctgtttggcg cgtgagtcca caaactaccg
     1381 aagctcaaat tccgaattcc gactgcgttt ccaaatcagg aattatcaat atttcaaaat
     1441 ttgtcgatgc aaacggtact ctttcttggc tttcgccaaa aggaaaatgg aggattattc
     1501 gaatgggaca cacttccaca gggcacgaaa atgccaccgc tggagcagca agaggattgg
     1561 aagtggacaa gtttaatccc gaagcggttc gttttcagct ggatcattgg tttggagaaa
     1621 tgctgcgaat agccgggcca gaattggcat ccaaagtggt taaaattttg cacattgaca
     1681 gttgggaatg tggcagccaa aactggtcgc ctgtttttca agccgaattt aaaaaccgca
     1741 gaggttatga tttggtcgat tatttgccgg ttatggcggg aattcccatc gaaagtgccg
     1801 caacttctga aaaggttttg tacgatgttc gaaaaactat tgctgaatta gtggccgata
     1861 atttttttgg aaccttggcc aatatcgccc aaaaagccaa cgtgaaattc agttccgaaa
     1921 acgtggctcc aacaatgatg agtgacggac ttttgcattt caaacatatc gattatccaa
     1981 gtggggaatt ttggctgaaa agtccaaccc acgacaaacc caatgatatg cttgatgcga
     2041 tttcaggtgg gcatatttat ggaaaagata ttattcaggc agaagctttc accgagttga
     2101 aaatggattg ggacgaacat cccggaaatt tgaaaacttt ggcagaccga aattatgcca
     2161 tcggaatcaa ccgatttttc taccacgttt ttgcgcataa tccgtggacg gacagaaagc
     2221 ccggaatgac attggatggc gtgggttctt attttcaaag agaccaaact tggtggaaac
     2281 ccggaaaagc ctgggtggat tattgtcaga gagtacaatt tcagttgcaa aaaggaaaac
     2341 cggttataga tttggctgtt tttattggcg aagatttgcc gtcgcgttcg gtacttccgg
     2401 atcgattggt gccttttatt ccgaatattt tcgggaagga acgtctggaa agcgaagcca
     2461 ttcgattgaa aaacgaaggt cagccaagca ttaaaatgcc gaaagaagta acttcgacta
     2521 aaaactcaac cgatttgtcg caatgggtaa acgcgatgaa cggttaccaa tacgattcgt
     2581 tcaatgccga tgttttactg aataatgcca aagtcgaaaa cggaaaagtg attttcagca
     2641 atagtattca gtatggtgct ttgctgtttc cgggaagcca caaaatggcg ccgaacaaaa
     2701 tgatttcttt ggctgttgcc aaaaaaatat tggaattggt caaagatgga gcaactgttt
     2761 tcattgacga aaaaccaaat ttacaaccgg gaatatattc tgaagcggat cataaaaatt
     2821 ggcaaactac aatcgatgaa atttggaaca atacttcgaa tgcaacttct tggaaaatcg
     2881 gtcaaggaac aattattgaa ttgccgtatt taggaaatga ttttgctgaa atcggaatta
     2941 ctcaggatgt ttattttcca aaattaaacc gtgccgattc tgaaacttta gcctggacac
     3001 accgcaggtc tgatgaggaa gatgtttatt ttctatccaa tcaaaaagca gaaaaaagac
     3061 aatttgaagc ttcgtttcgt gtagtgggaa aaattccggt ctggtacaat ccggttaccg
     3121 ataaaacttc ggcattggcc aattggaaaa ttgaaaacgg tagaacaatt gttgctttgg
     3181 ctttagatgc caatgaatcg ggtttcgtga tttttaaaga agaaactaaa gaagttcagg
     3241 caaaaggcta ccaaaaaggt ttggagtttg aaaacgttca gactttggat gaaaattggg
     3301 aattacagtt tgattccgaa tataaaggcc cgaaagaagt tgtaaaaacc aataaattgt
     3361 ttgattggtc aacatcagaa aataaccaaa ttaaatacta ttcgggtacc gttatttata
     3421 aaaaagattt tgtttggaac ggaaaagata cgaataatct ttggttggat ttagggacaa
     3481 ttgccaatat ggctgaggtt agcatcaacg gaaaagattg cggaacactt tggacttttc
     3541 cttataaagt ggatgtttca aatgcattga aaaaaggtaa aaacaccatc atcatcaaaa
     3601 tcacgaatac ttgggcaaac cgattaatcg gcgaccaaaa attgcccaaa gaagaaagat
     3661 taacttggac aacggctcct tttagattgg aaggaaatcc attgttgaaa gcaggattgt
     3721 tgggaccagt aacgattgta aaagaaaaaa gtaaaagaaa tgaataaatt tagaaatcat
     3781 attattgcca ttgtattgct tttaacaagc tttcaaatca acgcaaaaat caaattgccg
     3841 gctttgtttt cggacaatat gatgttgcag caaaagtcga atgcgccaat ctggggctgg
     3901 gctgaaaaga acgcgaacat tacgataaaa acatcttgga atgcaaaaat atacaaagta
     3961 aaagcagata attctggaaa atggaaaaca gaattgcaaa ccccatcttt tggagggcca
     4021 tttacaattg aagtatcgga aggagctgaa aaagtggtta tcaaaaacgt tttggttgga
     4081 gaagtttggt tgtgttcggg acagtcgaat atggaaatgc ccttgaaagg gttttctggt
     4141 cagcctgtga gaaacggtaa cgaaatcatc gtgaaatcaa ccaatgcaac cattcgtttg
     4201 attacgattc cgcgcgctac agttttgcaa cctttggata attttgaagg aaaatgggaa
     4261 gtggcttcgc caaaatctac agctaatttt agcgcaacgg cttggtattt tggttcgctt
     4321 ttgcaggaag tattgcaggt tcccgtggga ttgattcacg tttcgtatgg aggttcaagt
     4381 atggaagcct ggatgaatca ggaaatgctg aaagattttg caagtgccaa aatcccaacg
     4441 acaaaagaag aattggccaa agacccaaac cgtgttccta cgaccttgtt taacggaatg
     4501 ctttcgccgg ttatcggtta cggaatcaag ggttgtattt ggtatcaagg cgaatcgaat
     4561 tacgaacgag cttcggaata taccgttttg atgaaaaaga tggtttcgag ctggagagct
     4621 atgtggaagc aaggagattt tccgttttat ttctgccaaa ttgcaccatt taattacgcc
     4681 tcttttcatc caaaagatta tctggaaaaa tacaattcgg cttatttgag agaagcacaa
     4741 ttgaaagctt caaaagaaat tccaaataca ggattggctg ttttgatgga cgttggcgaa
     4801 gaaaataata ttcacccaac cgacaaagaa aaaggaggaa accgtttggc ttttcaggct
     4861 ttgagtaaaa catatggaat tgaaggcttc gagtttgaaa gcccgaaata taaggcaatg
     4921 gaaataaaag acaattcggt tacagtttct tttgacgatg tcccaaatgg gattacgtct
     4981 tacggaaaag aggtgaacgg ttttgaaatc gcgggagaag acaaggtttt ttatcctgcc
     5041 aaagcggaag ttcgtagaaa atcggtggtt ttgacttcgg acaaagtagc gaaaccggtt
     5101 gccgttcgtt atttgtggaa ggattttgta aaagcggaat tattcagtaa tggcggattg
     5161 ccgatgtcgt cgtttaggac ggatgagtgg tagtttgcag ttatgagtta agggttatga
     5221 gttatgagtt ttcgtttcgt ttgtcattcc tcgttcctcg gaatgacaaa aacacaaaaa
     5281 aacaaaggaa attgaaatac ataaaataca tacttctctt cactacgttt ttagtcaaag
     5341 cgcaaattcc cgtgcttgat aattacaacc aaatatggac aacccaaagc gcctattcct
     5401 tcgagtcgat gcctttggga ggtggcgata ttggcttgaa tgtttgggta gaaaaagggg
     5461 atttatactt ctatttttcg cgaagcggaa cttttgacga acataatacg ttgctgaaat
     5521 taggtcgcgt aaaagttagc ttgtcgccta atccatttac cggagaagaa ggttttcgtc
     5581 aagaactgaa actcaaagat ggttgtattt tgattggaca aaatgatgtt aaaatcaaac
     5641 tttgggtcga tgttttcaag ccgataatcc atttggattt agaaagtgaa aatccgctga
     5701 aaatgacggc ttcctacgaa agttggcgtt acaaaaacct agattccaaa gggaaagcca
     5761 ataatgccaa ttcctataaa tgggctccgc agggagatat tgtcacttac aaagattcga
     5821 ttgcttttga aaataatggg atcaagtttt accaccgaaa ccgtgaacaa accgtttttg
     5881 acgttgcggt aaaacaacaa aaaatggaat cggtaaaaga ccaaatgatg aacccgattg
     5941 cgaatttgac tttcggggga tttatggtgg gcgaaaattt gaaaccggac ggaacatatc
     6001 ttggaaaata tcaggaaacc gattgtaaag gttttaattt atcaagcata aaaccttcca
     6061 agaaacactc tttggaaatt tatttgaata caagtcaatc tgatttttct acttggaata
     6121 acggattgaa aaatcaaatt tcggagtata aaaccaaagg aaaacaagcg gagaaaaata
     6181 cccaaatttg gtggaataat ttctggaaac gcagctttat ttttacccaa aaaaatcaat
     6241 caacagccaa agattcggtg tatcaaatcg ggcagaatta tcaattgttc cgctatatgt
     6301 tgggttgcaa tgcctacgga aaatatccga ccaaattcaa cggaggattg tttacagttg
     6361 atcccgtttt taccaatccc gatttgaatt tcacgcccga tttcagaaat tggggtggcg
     6421 gaacgatgac cgcccaaaat caacgcttgg tttattatcc aatgacaaaa agcggtgatt
     6481 ttgatatgat gaaatcgcag ttggattatt atgtaagcct tcagaaaaat gccgaattgc
     6541 gttcgaaagt ctattggaaa cacggtggag cggcatttac ggagcagttg gaaaattttg
     6601 gcttgccaaa tcctgccgaa tacgaatgga aacgtcccac agattacgat cggggaatgg
     6661 aatacaacgc ttggttggag tacgaatggg acaccgtttt tgagttttgc aaaatgatgt
     6721 tggaacagaa agaatatgcc aatgaagata ttcaaaaata caatccgttc atcgtcagtt
     6781 gccttcgttt tttcgatgaa cattaccaat atttggcgaa acaaagaggt cgaaaagcct
     6841 tggatggcga tggacatttg attctttttc cgggttcggg agcagaaacc tataagatgg
     6901 caaataacgc caacagcacc atttcggctt tgaccgtaat aaccgaaaag attttaaccc
     6961 tttcggataa agaattgtcc aaggaagacg tagattattt gaaaggcttt caaactcgaa
     7021 ttccaccttt gaatttcaga gaatttgacg gaaataaaac attggcgccg gccaaatctt
     7081 gggaacgcat caacaattcc gaagtgccgc agttgtattc tgtttttccg tggggaattt
     7141 acggcattgg aaaactcgat ttggaaaccg ctttaaatac ctggaaatac gatacggatg
     7201 caattaaatt cagaagtcac gtaggttgga aacaggataa tatttttgcg gcacgattgg
     7261 gattgaccaa ggaagcagcc aaatataatt tgctaaaaat ggcaaattcc gaaagacgtt
     7321 ttccagcttt ttggggacca ggatacgatt gggttccgga tcataattgg ggaggaagcg
     7381 gaatgattgg aatgcaagaa atgcttttgc aggaagcgaa tgggaaaatt tatctttttc
     7441 cggcttggcc aaaagactgg aatgtacatt tcaaattgca cgctactcaa aacacaacta
     7501 ttgaagcgga acttataaat ggtgaattga aaattttgaa agtaattccg gaagaaagaa
     7561 aaaaagatgt aattaatctg cttgacaaat cagaggcaga aaaaataaaa ttgaattaag
     7621 atgataaaga aaatgatggg tgctgcggct ttggcaatta ttttggcaac aaacggacag
     7681 gcacaaaaca ccgaaaaaca aatcgtgaaa gtagatttcg atttttcgca acgaagaatc
     7741 gacgaagtga atgatgtcac ttatagttcc tggcccataa gcgagcagaa acaagacgag
     7801 aaaagtttta acggtttgac ttttaagttg aaaggaaatt tcaattcgaa atggtacaaa
     7861 gtgggaatga atgcgccatt ttacagcaaa ttggcgagcg atggtttggc tacagccgaa
     7921 aacttggaat taaccatcag tggactgaaa gcgggacaac actcgttgct gacttttcac
     7981 aacactttcg ataaaatcga aggcaaaacc ttttctcccg taaaaattta cgtgaatgat
     8041 aaattggcag aaacagtttt gcaaagcaat cgttctaatt ccaaaattga aaccgcaaca
     8101 gcttatattt tgtttaatgc tgaagcaaac aaggatgtgg tgattcgttt cgaattggat
     8161 gcaaaagcaa ccgacttgat ccaacaaatg atcatcaacg gatttgaaat agacactcca
     8221 aatttgaaaa agcaatcgca tacgccaaag cctgaaaatg ctaatgagca cgtggtgatt
     8281 gacaaaaatt tggttttgaa ttgggaatcg gcaaaagatg cggtggcgca ccaaatctat
     8341 tttggaacgg atagcaatgc cgtttcaaat gccaatgata aatctcctga atttaaagga
     8401 aaattaaccg agaacaaatt tgatcttgga aaattgtaca gcggaacaac ttattactgg
     8461 cgtgtggatg aattggatgc caaaggaatt gtgaccttgg gaacggtttg gtcattcaag
     8521 ccagcgcaat tggctttccc tgaagcggaa ggttacggac gttttgccgt tggaggaaga
     8581 ggagggaaag tggttgaagt aacgaacttg aatgatagtg ggccgggaag tttgcgtgat
     8641 gctgttgatc aacaaatagg gcggaaaacc attgtgttta atgtttcagg aaatatcaaa
     8701 ttagcctcaa ggttggtggt gaatcaacct tatgttacta ttgcgggaca aactgctcca
     8761 ggcgaaggaa ttacaattac aagagcacca attggattga ctggaaatga tggtgttgtg
     8821 cggtttttga aagtgagaat tggtggcgga accacttatg acggaatggg acttaccggt
     8881 gccaatcaca gtattataga ccactgttcc atcagttgga ctattgacga atcgttcagt
     8941 tctcgtggag cacacaatat taccttgcaa cgtaccttga tctcggaagc attgaatgtg
     9001 gcgggtcacg acaaatatcc agcaggaaaa atgcacggat acgctgcaac cattgggggc
     9061 gatatcggaa gttttcacca caatttattg gcgcacaatg aaggtagaaa ctggagtatt
     9121 ggtggtggtt taaacggtga tggctattac gggggaaaaa tagacatcag gaataatgtg
     9181 gtctataatt ggggacatcg tgccacggat ggcggtgcca atcaggtaaa tttcgtgaat
     9241 aatttttaca aaccgggagc ttccaccaaa atattcgtgg ctttgaatgc ccaaattgaa
     9301 ggagttggca aaggaaaaca acaatattat tttgacggaa atacaatgcc tgggtatttc
     9361 aacgaaaaga atcaagagaa aggacgaaaa ttcactttgt cgaataaagc cattgtagat
     9421 tatgaggtgt ttttgaataa accatttttt gaatcctatg taaacaccca aactgcagat
     9481 gccgcttata aaaacgtgct ctcggatgtg ggagccaatg cgccgttttt tgataaacac
     9541 gacaatcgta tcattgatga aacgcttaaa ggaacttaca cttataaagg aagcaaaagt
     9601 ggattgggtg gattgattga caacgaagcc gatcaaggag gttggccaaa ttttgcaact
     9661 gaaaatcgtc cagctgattg ggataccgac cacgatggat taccaaattg gtgggaaaaa
     9721 gcaaaaaggt tgaatgagaa ttcaaaaaat ggagattttt cagatgctgg tttagatgca
     9781 gataaagacg ggtttaccca attggatgac tatttagatt ggatggcaca gccgcacttt
     9841 tttgtaaaaa cgggtgaaaa attaaccttg gatgctgcaa tttatttcaa aggatatgag
     9901 aaaaaaccag tttatacttt ttcggaagtt aaaaatggaa aagcagtttt aaaaggttca
     9961 actattaaat ttacagctga atccaaagat tttgcatcat tcaaaataac cgtaactgat
    10021 aaagacggag acacgatgac tagaactatc aatgtatttg taaaataaaa ggctaccgca
    10081 aatccgtaaa tttctatagc aaagtagttt tcacaatttg cgaatttgcg gtagattttt
    10141 tttaagattt aaataaaaca cagatgaaaa aacttattct aaccgctcta cttttttcca
    10201 acgctttcgt tttttctcaa aacaaaggtt tggtagccaa ttcggatagc ccatattcca
    10261 aattacaaag cgtggcaatg caggatgtca aatggacgaa cggtttttgg aaagaacaat
    10321 ttgatgtgga aaccaaaaat accttgccgt atatgtggga tttgtatcat aacgatgaag
    10381 tgtctcacgc ttatgccaat tttaaaattg ctgctggcga aagcaagggc actttcaagg
    10441 gaccttcgtt tcacgacgga gatttctaca agattttcga aggaatggcg gccacttatg
    10501 ccgtaaccaa agacaaaaaa ttggatgccg aaatggacaa agccattgcg ctttttgcca
    10561 aagtgcaacg caaagacggc tatttgcaca cgccggtttt aatcgacgaa cgttggggaa
    10621 cattggggcc ggaagaagtg aaaaaacaat tgggtttcga gaaatacaat atgggacatt
    10681 tgatgaccgc cgcctgtatt cattatcgtg cgaccggaaa aaccaatttt ctgactattg
    10741 ccaaaggcgt tgccgatttc ttgtatgatt tctacaaaaa agcttcgcca gagttggctc
    10801 gaaacgccat ttgtccttct cattatatgg gaattgtcga aatgtatcgc acgaccaaaa
    10861 acccgaaata tctggaattg gccaataatc taatcgatat cagaggaacg accaacgacg
    10921 gaaccgacga caatcaagac cgaattccgt tcaggcaaca aaccaccgca atgggtcacg
    10981 ccgtgagagc caactatctg tacgccggcg ttgccgattt gtatgccgaa acgggagaga
    11041 aaaaattatt ggacaatctg gaatcgattt gggatgacgt tacttatcga aaaatgtaca
    11101 ttacaggagc ttgcggttcg ttgtatgacg gagtttcacc tgacggaact tcgtataatc
    11161 ctactgatgt gcaaaaaatt catcaggctt acggaagacc ttttcaattg ccgaatgcca
    11221 ctgcccatac cgaaacctgt gccaatattg gaaatgtgtt gtggaactgg agaatgctcc
    11281 aaattactgg cgatgccaag tatgccgaca ttgtggagtt ggctttgtac aacagtgttt
    11341 tgtcaggaat cagtctggaa ggaaaagagt ttttctacaa caatccgttg aatgtttcca
    11401 agaatttgcc tttcaaacaa agatggagca aagagcgtga aggttatatc gctttgtcca
    11461 attgctgtgc gcctaacgtg accagaacca ttgccgaggt gaacaattac gcttacagtc
    11521 tttcgaaaga aggattgtat gtgaatttgt atggaagtaa taatttgaat tcaacgactt
    11581 taaacggaga aaaaattgaa atagaacaac aaacaaatta tccttgggac ggaaaagtta
    11641 gtctgaaaat cgtgaaagct ccgaaagata tttatgcttt tttcttacga attccaggtt
    11701 ggagtcaagg aacaacgatt tccattaatg gtaaaaacat aaatgatgca attgcttcgg
    11761 gttcgtacca aaaaatagcg caaaaatgga aaaaaggaga tgtcatcgaa ttgaatatcc
    11821 cgatgccggt ggagttgatg caagccaatc cattggtaga agaagtcaaa aatcaggttg
    11881 ctgtaaaacg tggaccaata gtgtattgct tggaatcgaa tgaattgcct gcgaacacaa
    11941 aagtgaacga tgtgattttg gatgtcaatt ctaaatttac taccgatttt atcaaaattg
    12001 ataaccgaca attgttgagc attaccgcca gttcatccat tattccagat aattcttggg
    12061 acaaaaaatt atacaaaccc atttctatca aagacaacaa ggaatatacg gtaaaattga
    12121 ttccttattt tgcttgggga aatcaaggca agggagaaat gtcggtttgg ttgtcgcatt
    12181 aatgtttttt gcacactaaa tttttctctg cattttcaaa ctgcaataat ggtaaaacaa
    12241 ggtgtggtca tcaaaaatta gaagtaaatg accaaaattt gaaagatgtt attactctaa
    12301 taaaatattt ttgatgcaaa ataggattat taatcgaatt taggttacga gaaaatattt
    12361 taaaacaaag cactaaccaa aaatgaaaaa aacaataacg ttcctattgt tgttgacaat
    12421 agttcaaatt caagcacagg aatttatgcc actttggaca aaaggaaaga tgcccaattc
    12481 caaaggattg gtcgtaaaag acagcatcaa caatgagcga gtgatgcagg tgggaatgcc
    12541 gggcattcac gtttttttta cttctattca agagaacaaa ggagcagcgg ttttgattat
    12601 tccgggaggt ggttaccatc atttgtcgta taatatcagc ggatttcagt tggcaaaatg
    12661 gttcaacaca atgggaatta acgcttttgt attaacacat cgattgccta tttcgcctga
    12721 cctcaaagaa agagaaaaag cacccttgca ggatgctgaa agagcgatgc gcatcattcg
    12781 aaaaaatgcc gtgaattgga aaattgacac taacaaaatt ggtgttatgg gttgttcggc
    12841 aggagggcat ttggcggcta gcctcgggac aatcaaagat gattttgcta aaattggaga
    12901 tgattttgac agtttttcgt ttagacctga ttttatgata ttggtttcac cagtaattga
    12961 tatgggaaaa gaaactcata aaggaagtct tgaaaatttg ttaggtaaaa atccatcgtc
    13021 tgaattaata aacaaatatt cattgcaatt tcaagtgagc aattatacgc caccaacgtt
    13081 tattacccac gctttcaatg ataaagtcgt gtcgccaaag aacagtttgc tattttataa
    13141 tgcattgtta gaaaacaaaa tcccttcgag tttgcatatt ttcaatcaag gcgctcacgc
    13201 cattgctttg cgaaataatc ccggctcgac taatatttgg acagctattt gcgaacaatg
    13261 gttgatggaa ataaatattc ttaaaaacta aaatttaaaa ctaactacaa atcatttatg
    13321 aaacattttt taaccacatt gctatttttg accacgtttt ttttaacggc acaaaccacc
    13381 gataatctat ttgcattaac tgttgcccaa gatggttccg gcgatttcaa aacgattcag
    13441 gaagccatca ataaagtgag agaccacgcc gaaatgagag tggttattac cattaaggcg
    13501 ggaacttaca acgaaaaagt ggtcattcca tccttcaaga gaaacattac tttgagagga
    13561 actgacagag aaaaaacgat tatttcttat gacgattatt ccggaaaacc gttccgaggc
    13621 attgatgtaa cgggtaatga aaagtacagt acttatacat cttatacgct attggttcaa
    13681 ggaaatgatt gttcggtaga aaatttgact atccaaaaca cggcaggtag agtaggtcaa
    13741 gctgtggctt tgcatacgga aggcgataga gtggctgtta aaaattgttc cattttaggc
    13801 aatcaagata ccttgtattt agccaaaggc ggaaccagaa attatttcga aaactgtt
//
LOCUS       DAOSNN010000039        12541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5017_length_12541_cov_5.289604, whole genome shotgun sequence.
ACCESSION   DAOSNN010000039 DAOSNN010000000
VERSION     DAOSNN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5017_length_12541_cov_5.289604"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLQ70_03475"
     CDS             complement(<1..748)
                     /locus_tag="PLQ70_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33706.1"
                     /translation="MKKKMLFMSLLLLLFATYSCAIEELQPETTPISSEILNNVNPEF
                     LEHDLSVYKIKPIWKSAIVFKSSSAANKKTKFNEAVEVNFTQDNKTTIPISKNGKIRG
                     RQRLLLTFEDGKIRETIIEYIPSDSFMGDIKTINSGNFKSKQFDGKITFQNPKAQAKI
                     VWYLTKGKLVRKARSSTKKTKTSKTARWVSVFECHTNWVCVGEGADEKCEEKIYCGWE
                     EVWEEDDPGNGLPPDDPYDCEVDPSWPWCQD"
     gene            complement(761..1537)
                     /locus_tag="PLQ70_03480"
     CDS             complement(761..1537)
                     /locus_tag="PLQ70_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33707.1"
                     /translation="MNHKNIIPQYIWLFSFLLFFTNCKNETDSKNPIQIKQSSIVMSV
                     SDKKTIEYYRNEIDNAVSLLAKQKKIIDVNLNKNEKVEIIAIAFPEILRYNAFSDLIE
                     TTSNRLLYINRGKTASDFSIGLFQMKPSFVEDLENYVANSEKLEKYKNIQIQNKTEKA
                     TREERVNRLENFHWQLYYLKIFWAVMDDKYSQVTFENKKDKLRFYATAYNYGFMRPIQ
                     EILSFQYQKIFPYGSDKKNALRFADFSIDFCNNYNSLFNQ"
     gene            complement(1839..2657)
                     /locus_tag="PLQ70_03485"
     CDS             complement(1839..2657)
                     /locus_tag="PLQ70_03485"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HQE33708.1"
                     /translation="MKNQIFSAILFGIISLPLFSQTKDETIFIEKNKIELASTPSESI
                     IKIAKSFLETPYVASTLEINENEQLVCNLSELDCFTFDENVLALYLTKKKNSNSYADF
                     QEILTQLRYRNNKIDGYASRLHYFTEWVRQAEKNGYIKDITLEIGGEISTKEVRFMSD
                     NQKLYPKINDGKTLKTIVKNEEKINKKPLSQIEKSKVASIESKIKEGDFIAMTSTLEG
                     MDCNHVGIAILVNNRIHLLHASSSLKKVVISEKPLAEYLEAIKKDAGIMVLRVL"
     gene            2728..3222
                     /locus_tag="PLQ70_03490"
     CDS             2728..3222
                     /locus_tag="PLQ70_03490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQE33709.1"
                     /translation="MKAIEKMTIEKTTYQDDFDFCALMMAKSDPWITLEIDFNLCVQA
                     FAGASKEVYVIRINSVIAGFVILQIDGTFKGYIQTICINENYRGKGLGKKLLTFCEER
                     ILKISPTIFICVSSFNTRALQLYTDFGFKIIGELPNFIREGFTEILLWKTFGPKIGYS
                     NSEK"
     gene            complement(3315..4940)
                     /gene="groL"
                     /locus_tag="PLQ70_03495"
     CDS             complement(3315..4940)
                     /gene="groL"
                     /locus_tag="PLQ70_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989998.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQE33710.1"
                     /translation="MAKDIKFDIEARDGLKRGVDALANAVKVTLGPKGRNVIIGKSFG
                     GPNVTKDGVTVAKEIELKDPLENMGAQMVKEVASKTNDLAGDGTTTATVLAQAIVKEG
                     LKNVAAGANPMDLKRGIDKAVEAIVADLAKQAKVVGSDSDKIKQIASISANNDDVIGE
                     LIANAFAKVGKEGVITVEEAKGTDTYVDVVEGMQFDRGYLSPYFVTNAEKMEAELENP
                     YILLYDKKVSSLKELLPVLEPVAQSGKPLLIIAEDVDGEALSTLVVNKLRGALKIAAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEERGYTLENTTIEMLGTAKRVTIDKDNTTIVS
                     GAGDAEMIKNRVNQIKGQMDATTSDYDKEKLQERLAKLAGGVAVLYVGAASEVEMKEK
                     KDRVDDALHATRAAVEEGIVAGGGVALLRAKNALADLKADNADEATGIKIISRAVEAP
                     LRTIVENAGLEGSVVVAKVAEGSGDFGYNAKTDEYVDMLKAGIIDPKKVTRVALENAA
                     SVSGMILTTECALIDIKEESAPHPMGGGMPGMM"
     gene            complement(5023..5298)
                     /locus_tag="PLQ70_03500"
     CDS             complement(5023..5298)
                     /locus_tag="PLQ70_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQE33711.1"
                     /translation="MALNIKPLSDRVLIEPVAAETKTASGIFIPDTAKEKPQKGTVVA
                     VGNGTKDHEMTVKIGDTVLYGKYAGTELKLEGKDYLIMREDDILAII"
     gene            complement(5440..5790)
                     /gene="secG"
                     /locus_tag="PLQ70_03505"
     CDS             complement(5440..5790)
                     /gene="secG"
                     /locus_tag="PLQ70_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQE33712.1"
                     /translation="MSTFSIFLALITIVCFLLVVVIMVQNPKGGGLSSSLGGSTQIGG
                     VQKTTDFLDKSTWTLGTILILLILLSSLSFGGSLGTSESKIIEKTEAITPKAATPAPA
                     TPATAPAPATEPAK"
     gene            complement(5793..6671)
                     /locus_tag="PLQ70_03510"
     CDS             complement(5793..6671)
                     /locus_tag="PLQ70_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE33713.1"
                     /translation="MNVTDYTYLINKPDTISEKQTDALGIVLDEFPYFQSARALRLKG
                     LFNQNSFRYNYALKVTASHTTDRTVLFDFITSDTFVAIQKGLYDKKVLELLDISVVDC
                     EIIKPEIPLEPKINTIEQSILTTIKEATGIEEESNTKIAEENLEIGKPLGFARDEKHS
                     FQEWLQLSRIQPINREPETNKINELDQKSVAKTVPVEDEKKKKAELIDKFIEASPKIS
                     PIKQSTETTFHIDLNKSDNSYLMTETLARVYLEQKKYLKAIQAYEILILKYPEKSSFF
                     ADRISDIKSLQQNNNN"
     gene            complement(6738..7241)
                     /locus_tag="PLQ70_03515"
     CDS             complement(6738..7241)
                     /locus_tag="PLQ70_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HQE33714.1"
                     /translation="MKHLKYIIALSILFTFGGCNVYNFTGTGKIDAKTFQVNFFPNTA
                     ELVQPGIDRTFTLALQDLIQNQTNLNLVKNGGDLTYEGEIVDYRITPMMATADIQAAQ
                     NRLTIRVSVQFTNKNKEADNFDKTFEFFKDYPAKEQLSGSTLDAAVKEIFERITQDIF
                     NESLAKW"
     gene            complement(7301..8581)
                     /locus_tag="PLQ70_03520"
     CDS             complement(7301..8581)
                     /locus_tag="PLQ70_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQE33715.1"
                     /translation="METVQSIKQRFEIIGNDPKLNRAIEKAIQVAPTDISVLVVGESG
                     VGKESIPKIIHSLSHRKHGKYIAVNCGAIPEGTIDSELFGHEKGAFTGATSTREGYFE
                     VASGGTIFLDEVGELPLTTQVRLLRILENGEFLKVGSSQVQKTDVRIVAATNVNLFDA
                     IEKGKFREDLYYRLSTVDITLPPLRDRKDDIHLLFRKFAADFAHKYKMPPIKLDENAI
                     QLLQKFRWGGNIRQLRNVAEQISVLETNRDISAITLQSYLPSESSNLPSVIKDKKAEN
                     DFSTERDILYKVLFDMKSDLHDLKKLTLELMKNGSAKAQDINANLIQKIYGSKETESE
                     ISFEEQPVSTIFSAESRSLSTPQIQENNFVENEEDFQFAETVEEEEILKLEQREIEMI
                     KKSLEKNKGKRKAAADELGISERTLYRKIKQFDL"
     gene            complement(8681..8827)
                     /locus_tag="PLQ70_03525"
     CDS             complement(8681..8827)
                     /locus_tag="PLQ70_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33716.1"
                     /translation="MTKKNIIIAILTFIIGFGSTFYIIRTYFPKHKVEKTVPAKDTLT
                     KTKN"
     gene            complement(8931..9302)
                     /locus_tag="PLQ70_03530"
     CDS             complement(8931..9302)
                     /locus_tag="PLQ70_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013686509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HQE33717.1"
                     /translation="MEHYREDETYKIIGICMEVHRILGPGLLEIVYKDALEIEFRENN
                     IPYEREKEFAIEYKGKILPHRFYADFIVNGDIILEVKAVKEFSNEHIAQVINYLKLAD
                     SEIGLMVNFQTKSLQYKRYVL"
     gene            complement(9377..10822)
                     /gene="miaB"
                     /locus_tag="PLQ70_03535"
     CDS             complement(9377..10822)
                     /gene="miaB"
                     /locus_tag="PLQ70_03535"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQE33718.1"
                     /translation="MEKIIDENKQGESLILDYKPENTKKLFIESYGCAMNFSDSEIVA
                     SILSSNGYNTTQILEEADLVLVNTCSIRDKAEQTIRKRLEKYNAVKRTNPKMKVGVLG
                     CMAERLKSQFLEEEKIVDLVVGPDAYKDLPNLLSEVEEGRDAINVILSKDETYGDISP
                     VRLNSNGVSAFVSITRGCDNMCTFCVVPFTRGRERSREPQSIMKEIQDLWDKGFKEIT
                     LLGQNVDSYLWYGGGLKKDFTNASEMQKATSVDFDQLLEMVAVGYPKMRIRFSTSNPQ
                     DMHESVLHVIAKHSNICKHIHLPVQSGSDRILKEMNRLHTREEYMTLIDKIRTIIPDC
                     SVTQDLISGFPTETEEDHQDTLSLMEHVKYSFGYMYAYSERPGTLAERKMEDDVPEPT
                     KLRRLQEIVDLQRNHSAFRTQEFVGQTVEVLIEKASKKSEKDWSGRNSQSIVVVFPKE
                     NYKIGDFVNVKISSCTSGTLIGEAVGLSEMN"
     gene            complement(11115..>12541)
                     /locus_tag="PLQ70_03540"
     CDS             complement(11115..>12541)
                     /locus_tag="PLQ70_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HQE33719.1"
                     /translation="RIELKNHPKLTQLASDGNYFTQEEIKNIVKYADERGIMVIPEID
                     VPGHGTAILTAYPEVGSKLNFDKNTYSLERNSGIFTPTLDPSNPKTYQLLSEIFDEVC
                     PLFSGNYFHIGGDENEGKDWDSNPKIQEFKKKNNFANNHELQTYFTMQLIPMLKKHNK
                     TLMGWEEIMTKNLSKDAIIHSWKGSNEGIAAGQSLVNAVKNGYKTVLSNGYYIDLMLG
                     VENHYLNDPMPKNITLTAEEKARILGGEAPIWSELVTPTTIDSRIWPRTAAIAERLWS
                     NENITDMASLRKRMNVISLQLEELGITHIRNKEVLLRNISNNQTNTALEDFSNICEPL
                     KLYTRNSEGTEYKTYSPFTLFADACTPDATDALDFDEAVNNYLKNKSTENQILVTNYL
                     KKWISVNTSLIELSNNAPLIQPLLPLSKSLSELSQQLLLTLENKQVVDPGKLKNLLEQ
                     CNSKEHADVELAVYNSLKKLISAE"
BASE COUNT         3636 a         2212 c         2061 g         4632 t
ORIGIN      
        1 catcttgaca ccaaggccaa gaaggatcaa cttcacaatc ataagggtcg tctggtggga
       61 gtccgtttcc tggatcgtct tcttcccata cttcttccca tccacaataa attttttctt
      121 cacatttttc gtcggcacct tcgccaacac atacccaatt agtgtggcat tcaaagacag
      181 aaacccatct ggccgttttt gatgttttgg tttttttggt tgagcttctt gcttttctca
      241 ctagtttccc ttttgttaaa taccaaacta ttttagcttg tgccttaggg ttttggaacg
      301 taatttttcc atcaaattgc tttgatttaa aattgccact attgatagtt tttatatcgc
      361 ccataaaact gtctgaagga atatattcta taatggtttc tcgaattttc ccatcttcaa
      421 atgtaagtaa taatctttgt cttcctcgaa tttttccgtt ttttgatatt ggaatagttg
      481 ttttattgtc ttgggtaaaa ttgacttcaa cagcttcgtt aaattttgtt ttcttattgg
      541 cggctgagga ggacttaaat actattgccg atttccaaat aggcttgatt ttataaacac
      601 ttaaatcgtg ttcaagaaat tcagggttta cattattcaa tatttcactt gaaattggtg
      661 tggtctcagg ctgtagttct tcaattgcgc aagaataagt tgcaaacagt aacaacagca
      721 aactcataaa taacattttt ttcttcataa ttataatggt ttattggtta aatagggaat
      781 tgtaattgtt gcagaaatct atagagaaat cggcaaatcg caaagcgttt ttcttgtcac
      841 ttccataagg aaatattttt tgatattgaa aagacaatat ctcttgtata ggtcgcataa
      901 agccataatt atatgctgtg gcataaaatc gcaatttgtc tttcttgttt tcaaacgtta
      961 cttggctata tttatcatcc atgaccgccc aaaaaatctt gagataatac agttgccaat
     1021 gaaaattttc taatctattt actctttctt ctcttgttgc cttttctgtt ttgttttgaa
     1081 tttggatgtt tttgtatttt tctaattttt cagaatttgc tacataattt tctaaatctt
     1141 ctacaaatga aggtttcatt tggaagagtc ctattgaaaa atcagaggct gtttttcctc
     1201 tattgatata caataatctg ttagatgtag tttctatcaa atcactaaaa gcattgtatc
     1261 ttagaatctc tggaaaagca attgcaatta tttcgacttt ttcattttta tttaaattaa
     1321 catcaataat tttcttttgc tttgccaaaa gagaaactgc attatcgatt tcgtttctat
     1381 aatattcgat agttttttta tcgctaacgc tcataacaat gcttgattgc tttatttgaa
     1441 tcgggttttt tgaatctgtt tcgtttttgc aattggtaaa aaacaaaaga aaagaaaaca
     1501 gccagatata ttgtggaata atatttttat gattcatacg gttaatatct aattattgcc
     1561 caagcataat ttatttgcta ttgataattt aattctatcc cgaaagtatg aagaagaaat
     1621 agtatgtggt gttttgaaat ttaaacttta acaagatgtg cagtttaact gcatattatg
     1681 ttaaaatttg agaaataatc ttgaggagaa taaaaataac cttaaaaaaa gtccgtttac
     1741 attagtaaac gaactttaaa gtgtaaaata taattttgat gctagctagc ctagtatgtc
     1801 gaaggaaaga gtttatatat aaacagaatt tacaatttct ataaaacccg caaaaccata
     1861 attcctgcat ctttcttaat ggcttcaaga tattctgcaa gcggtttttc tgaaattacc
     1921 acttttttta gtgaagaaga ggcgtgaagc aagtggattc ggttgtttac aagaattgca
     1981 attcctacgt gattgcaatc cattccttcc aaagttgagg tcattgcaat aaaatcgcct
     2041 tcttttatct tgctttctat agaggctact ttagactttt ctatttgtga aagcggtttt
     2101 ttgttgattt tttcctcgtt tttgacaatc gttttcaagg tttttccatc attaatttta
     2161 ggatacaatt tttgattgtc actcataaaa cgaacttctt tagtgctgat ttctccaccg
     2221 atttctaaag taatatcttt gatataacca tttttctcgg cttgtcttac ccattctgta
     2281 aaataatgca aacgcgaagc ataaccgtct attttgttat ttcggtaacg caattgagtt
     2341 aaaatctcct gaaaatcagc ataagaattg gagtttttct ttttggtaag atacaatgcc
     2401 aacacatttt catcaaaagt aaagcagtct aattcgctta aattacaaac caattgctcg
     2461 ttctcattta tttctaatgt tgaagcaaca taaggagttt ctagaaaact tttggcaatt
     2521 ttaataatac tttctgatgg ggttgatgct aattctattt tatttttttc aataaaaata
     2581 gtttcgtctt tagtctgaga aaagagaggc aaactgataa tgccgaataa aatagctgag
     2641 aaaatttgat ttttcataga aggattgtat aggatttcaa agctacaaaa aatgattata
     2701 tttgagcgaa ttctagttta aagacttatg aaagctattg aaaaaatgac gattgagaaa
     2761 actacatatc aagatgattt tgatttttgt gccttgatga tggcaaaatc tgatccgtgg
     2821 attacgctag aaatagactt taatttatgt gttcaagcct ttgcaggagc gtcaaaagag
     2881 gtttatgtta ttagaattaa ttctgtaatt gctggatttg ttatccttca aatcgatgga
     2941 acttttaagg gatatattca aacaatttgt atcaatgaga actatagagg aaaaggactt
     3001 ggtaaaaaac ttttgacttt ttgcgaagaa cgtattttga aaatttcgcc tactattttt
     3061 atttgtgttt cttcatttaa cactagagca cttcagcttt atacagattt tggatttaaa
     3121 atcattgggg aattacccaa ttttattaga gaaggattta cagagatttt gctttggaag
     3181 acttttggtc ctaaaattgg atattcaaat tctgaaaaat aatttttaca acttgaaatt
     3241 ttttaaacaa aaaactccga agctttgaaa cttcggagtt tttttatttt gaacttcgta
     3301 cttcgtactt ggaattacat cattcctggc attccaccac ccattggatg tggagcactt
     3361 tcttctttaa tgtcaatcaa agcacattca gtagttaaga tcattccaga cacagaagca
     3421 gcattttcaa gagccacacg agttactttt ttaggatcga taattcctgc ttttagcata
     3481 tctacatatt cgtcagtttt ggcattgtaa ccaaaatctc cagaaccttc ggccactttt
     3541 gcaacaacaa cagagccttc aaggcctgcg ttttcaacaa tagttctcaa aggagcttca
     3601 actgcacgag aaataatttt gattcctgta gcttcatcag cgttgtctgc tttcaagtca
     3661 gcaagggcgt ttttagctct taataaggca acaccaccac cagcaacaat tccttcttcg
     3721 acagcagcac gagtagcgtg aagtgcatcg tcaacgcggt cttttttctc tttcatttct
     3781 acttcagaag cagcgccaac ataaagaacg gcaactccgc cagctaattt agccaaacgt
     3841 tcttgcaatt tttctttgtc ataatcagat gttgtagcat ccatttgacc tttgatttgg
     3901 ttcacacgat ttttaatcat ttcggcatct ccagcaccac taacaattgt agtgttgtct
     3961 ttgtcaatgg ttactctttt tgcagttccc aacatttcga tggttgtgtt ttctaaagta
     4021 taacctcttt cttcagaaat tacagttcca ccagttaaga tggcaatatc ttctaacatt
     4081 gcttttcttc tgtcgccaaa acctggagct tttacagcgg caattttcaa agcaccacgc
     4141 aatttgttta caaccaaagt tgataaagct tctccgtcaa catcttctgc aataatcaac
     4201 aatggttttc ctgattgtgc tactggttct aaaacgggta acaattcttt taaagaagat
     4261 acttttttgt catacaaaag aatgtaagga ttctctaatt ctgcttccat tttctcagcg
     4321 ttggtcacaa aataaggaga aagatagcct ctgtcaaatt gcattccttc cacaacatcc
     4381 acataagtgt cagttccttt ggcttcttca acagtgatta caccttcttt acctactttt
     4441 gcgaaagcat tggcaattaa ttcaccaata acatcatcat tatttgcaga aattgaggcg
     4501 atttgtttga ttttatcgga gtcacttcct actactttag cttgtttggc aaggtcagca
     4561 acgatagctt caactgcttt gtcaattcca cgttttaaat ccattgggtt tgctccagca
     4621 gcaacgtttt tcaaaccttc ttttacaatt gcttgtgcta aaactgttgc agttgtagtt
     4681 ccatctccag ctaaatcatt ggttttagaa gctacttctt taaccatttg cgctcccata
     4741 ttttctaatg ggtctttcaa ttcgatttct tttgcaacgg taacaccatc tttggtaacg
     4801 tttggtccgc caaaagattt tccaataatt acattacgac cttttggtcc aagagttact
     4861 tttactgcat ttgctaatgc gtcaacacca cgttttagtc cgtcacgtgc ttcaatatca
     4921 aattttatat cttttgccat ttcttctttt agtttaattt agtttaaagt ttaaagttta
     4981 aggttgcgtt tcgaacttga aacgttaaac ctgaaacaaa atttagatta tcgcaagaat
     5041 atcatcctca cgcatgatta aataatcttt tccttctaat tttaattcgg ttccagcata
     5101 ttttccgtaa agaacggtgt ctccaatttt tacagtcatt tcgtggtctt ttgtgccatt
     5161 tccaacagca acaacagttc ctttttgtgg tttttctttg gcagtgtctg gaataaaaat
     5221 tcctgaagca gtttttgttt cagcagctac aggttcaata agaacacggt ctgaaagcgg
     5281 tttaatattt aaagccataa tgatataata aatgttagta ttaatttgat ggatatttag
     5341 atttcagaaa ttgtgccaac ccctaaaaac tgacattttt tcctaaaaaa aatgccagct
     5401 ttgacaggct ggcatttact gtatttttat agattactat tattttgctg gctcagttgc
     5461 cggagccgga gccgtagctg gagttgcagg agctggagtt gcagcttttg gtgtaatagc
     5521 ttctgttttt tcaatgattt ttgattcaga agttcctaaa gaacctccaa aactcaaact
     5581 tgaaagtaaa ataagtagaa ttaatatggt tcctaaagtc caagtgcttt tgtctaaaaa
     5641 gtcggtagtc ttttgtacgc caccaatttg tgttgatcca cctaatgagg atgaaagtcc
     5701 accaccttta gggttttgaa ccattataac taccactaat aggaagcaaa ctattgttat
     5761 taatgctaaa aaaatcgaaa atgtgctcat tgttaattat tgttattttg ttgtaaagac
     5821 ttgatatccg agattcggtc tgcaaagaaa ctactttttt ctggatattt caaaattaat
     5881 atctcataag cttgaattgc ttttagatat tttttttgtt ccaaatatac tcttgctaat
     5941 gtttcggtca ttaagtaaga attgtcgctt ttatttaggt caatgtgaaa ggtggtttcg
     6001 gtactttgtt ttatgggtga aatcttcgga ctggcttcaa taaatttatc aattaattca
     6061 gcttttttct ttttctcatc ttcaacagga actgttttag ctactgattt ttgatctaat
     6121 tcattgattt tattagtttc aggttctcta ttgatgggct gtattcttga aagttgaagc
     6181 cactcttgaa atgaatgttt ttcgtctcta gcaaatccta agggttttcc aatttccaga
     6241 ttttcttcgg cgattttcgt atttgattct tcctcaatac ctgtagcttc ttttatagtg
     6301 gtaagaatag attgctctat ggtatttatt tttggctcaa gaggtatttc gggtttaatt
     6361 atttcgcaat caactacact aatatcaaga agttcaagga cttttttgtc gtacaatcct
     6421 ttttggatag caacaaaagt atctgaagtg ataaaatcaa acaaaacggt tctatcagtg
     6481 gtatgtgaag cggtaacttt tagagcgtaa ttatatcgga aactattttg attaaaaagt
     6541 cctttcaaac gaagcgctct agcactttga aaatacggga attcatccag cacgattccc
     6601 aaagcatcag tttgcttttc gctgatagta tctggtttgt taattaaata agtataatcg
     6661 gttacgttca tattagtgtt cattgtttgg cgtttagtgt tccgcgttcg tctgaaaact
     6721 gaacattgcc aactatttta ccattttgct aaagattcgt tgaatatgtc ttgcgtaatt
     6781 ctttcgaaaa tttcttttac ggcggcatct aaagttgatc cacttagttg ttcctttgcc
     6841 ggataatctt taaaaaattc aaatgtttta tcgaaattgt cagcttcttt atttttattg
     6901 gtaaactgaa cactcactct aattgtcaat ctgttttgag ctgcctgaat atcagctgta
     6961 gccatcatgg gggtgattct gtaatctaca atttctcctt cataagttaa atctccaccg
     7021 tttttaacca agtttaagtt ggtttgattt tggataagat cttgcaacgc caaggtaaac
     7081 gttcggtcaa ttccaggttg cactaactcg gctgtattgg ggaaaaaatt gacttgaaat
     7141 gttttagcat caatttttcc tgttcctgtg aaattgtaaa cgttgcatcc gccgaaagtg
     7201 aaaagtatcg aaagagcaat aatgtatttt aggtgcttca ttttgtgttt aaaaaagtgc
     7261 aggtcagttt actaaagttt ggttcaaaga tattcaattt ttaaagatca aattgtttta
     7321 tttttcgata caaagttctt tcagaaattc ccaattcatc agctgcagct tttcgttttc
     7381 ctttgttttt ttctaatgat tttttaatca tttctatttc tctttgctct aattttagaa
     7441 tttcttcttc ctctacagtt tcagcaaatt gaaaatcttc ctcgttttct acaaaattat
     7501 tttcttgaat ttgtggtgtg ctcaaacttc tggattcagc cgaaaagata gtgcttacag
     7561 gttgttcttc gaatgaaatt tcgctttctg tttccttaga tccgtatatt ttttgaatta
     7621 aattagcatt aatgtcttgt gcttttgcag agccgttttt cattaattcc aaagttagct
     7681 tctttaaatc gtgcaaatca cttttcatat caaaaagcac tttgtataga atatctcttt
     7741 cggtcgaaaa atcattttca gcttttttat ccttaattac agaaggcaaa ttgctcgatt
     7801 ctgatggcaa ataagattgc aatgttatgg cagaaatgtc tctattggtt tctaaaactg
     7861 aaatttgctc ggctacattt cgcagttgac gaatgtttcc tccccaacgg aatttttgca
     7921 ataattgaat cgcattttcg tctaatttga taggaggcat tttgtattta tgggcaaaat
     7981 cggcagcaaa ttttcggaat aataaatgaa tatcatcttt tctatcacgt aatggaggca
     8041 atgtaatgtc cacggtgctc aatctataat acaaatcctc acggaatttc cctttttcga
     8101 tggcatcaaa taaattgaca ttggtggcag ccacaattcg cacatcggtt ttttgaactt
     8161 gggatgaacc tacttttaaa aactctccat tttcaagaat tctaagcaaa cgaacttgag
     8221 ttgttagcgg taattcacct acttcgtcaa ggaaaatggt tccaccgctg gctacttcaa
     8281 aatagccttc acgagtggaa gttgcgccgg taaaagcgcc tttttcgtgg ccaaaaagct
     8341 cgctatcaat cgttccttct ggaatggcac cacagtttac ggctatgtat tttccgtgtt
     8401 ttctgtgtga aagcgagtga ataatttttg gaatactttc ttttccaacg ccactttcac
     8461 ccaccaccaa taccgaaatg tcagttggag ctacctgaat ggctttttct atagcacgat
     8521 tgagtttcgg gtcattcccg ataatctcaa atcgttgttt tattgattga actgtttcca
     8581 ttttattttg tttaaaggtt taattgttta aaggtttaaa agttgtttgg gttgaatcct
     8641 attaaacaat cttaaactat tttaaactat ttaaactttt ttaattcttg gttttggtca
     8701 atgtatcctt tgcaggaact gttttttcta ctttgtgttt tgggaaatag gttctaataa
     8761 tataaaaagt ggaaccaaat ccaatgatga aagtaagtat ggcaataatt atattttttt
     8821 tggtcattat gttttagttt tttaaactct tttttgaata agaattagtg tgttagaatg
     8881 tttttttatt ttgccacaga ttaaatgatt ttcacagatt ttctatttta ttaaagaaca
     8941 tatcttttgt attgcaaaga ttttgtttga aaattaacca ttaaaccaat ctcggaatct
     9001 gctaatttta aatagtttat aacttgagcg atatgttcat tagaaaattc ttttacagct
     9061 ttaacttcta aaataatatc cccatttaca ataaagtcag cataaaatct atgaggtaaa
     9121 attttgcctt tatattcaat tgcaaactct ttttctcttt cgtaaggaat attattttct
     9181 cgaaattcaa tttctaaagc atctttgtaa actatttcta acaatcctgg cccaagaatt
     9241 ctatgaacct ccatacaaat tcctataatt ttataagttt catcttctcg atagtgttcc
     9301 atattgatgt ttactctgtc atagttaaat tgattaaaaa atctgtgaaa atcatttaat
     9361 ctgtggcgaa cttttcttaa ttcatctcac tcaaaccaac tgcttcccca atcaaggttc
     9421 cgctggtgca acttgaaatt tttacattta caaaatcacc aatcttgtaa ttttcttttg
     9481 gaaacaccac aacaatactt tgtgaatttc ttccagacca atctttctcc gattttttgg
     9541 acgctttttc tattaaaact tctacggttt gtccaacgaa ttcctgtgtt ctaaaagcac
     9601 tgtgatttct ttgcaaatcg acaatttctt gcaatcgcct caattttgtt ggttcaggaa
     9661 catcgtcttc cattttgcgt tcggccaatg ttcctggacg ttcggagtag gcatacatat
     9721 aaccaaaact gtattttacg tgttccatta aactcaaagt atcttgatga tcttcttcgg
     9781 tttccgttgg aaaaccagaa atcaagtctt gggttacgga acaatccgga atgatagttc
     9841 tgattttatc aatcaaagtc atgtattctt cgcgagtgtg caaacgattc atttccttta
     9901 gaattctgtc gcttccagat tgaactggta agtgaatgtg tttgcaaata ttcgaatgtt
     9961 tggcaatcac gtgcaaaaca ctttcgtgca tatcctgagg attggaagtc gaaaaacgga
    10021 tgcgcatttt tggataacca acagctacca tttcgagtaa ttgatcgaag tcaactgaag
    10081 ttgctttttg catttcggaa gcgttggtaa aatctttttt caatccgcca ccataccaaa
    10141 gatagctgtc cacattttgt ccgagaagtg taatttcctt aaaacctttg tcccacaaat
    10201 cctgaatttc cttcataata ctttgtggct cacggctacg ttcgcgacct cgggtaaaag
    10261 gcactacgca aaacgtacac atattgtcgc aacccctggt gatggaaaca aaagccgaaa
    10321 cgccattcga attcaatcga acgggagaga tatcgccata agtttcgtct ttggacaaaa
    10381 tcacgtttat ggcatcgcga ccttcttcga cttcggacaa taaattaggc aaatctttat
    10441 aggcatctgg gccaacaacg aggtcaacaa ttttttcttc ttcaagaaat tggcttttca
    10501 aacgttcagc catacagccc aaaacgccca ctttcatctt tggattagtg cgtttcacgg
    10561 cattgtattt ttctaaacgt tttcggatag tttgctctgc tttgtcgcga atagaacaag
    10621 tgtttaccaa aaccaaatcg gcttcttcaa ggatttgtgt agtgttgtat ccgtttgagg
    10681 ataggatgga agctacgatt tcgctatccg aaaaattcat cgcacagccg taactttcta
    10741 taaagagttt cttggtattt tcaggtttgt aatctaaaat aagactttcg ccttgcttgt
    10801 tttcgtcaat aatcttttcc atagtcactt tcaaaagtgt gattttatta agaatgcaaa
    10861 gttacaatta tttgaatgaa tatgacaaga tgtcagccga agaattaaca gaattttagg
    10921 gtaaattggg aatgtttaat tttgaatttt ttttcaaaat tttcaagcaa aaaaaaacct
    10981 agcaaaacta attgctaggt tcataatatt atttttgaat ctaacagtta ccgaatatgc
    11041 tagaaaaata atatttcaat ctaaaatcaa accaaactat aaaccaatta actaaactca
    11101 atttgaaatt atttttattc agccgaaatt aattttttca ggctattgta aacggctaac
    11161 tccacatcag catgttcttt ggaattgcat tgttctaata aatttttcaa tttgccaggg
    11221 tcaacgactt gtttgttctc aagggttaaa agcagttgtt gcgataattc gcttaaactt
    11281 tttgataaag gcaatagagg ttgaatgagc ggagcattat tactcaattc aattaaactt
    11341 gtatttacgc taatccattt tttcaaataa ttggtaacta aaatctggtt ttctgtgctt
    11401 ttgtttttca gataattatt cacggcttcg tcaaaatcta aagcatcggt ggcatctggt
    11461 gtgcaagcat cggcaaatag ggtgaaagga gaataagttt tatattcggt accttcgctg
    11521 tttcgggtat atagttttag aggttcacaa atattcgaaa aatcctccaa agcagtattc
    11581 gtttgattgt tgctgatgtt tctcaatagc acttctttat ttctgatatg tgtgattcct
    11641 agttcttcta attgcaaaga aataacgttc attcttttgc gtaagctagc catatcagta
    11701 atgttttcat tagaccataa tctttcggct atggcagcag ttcttggcca gattctggaa
    11761 tctatagttg tcggagtgac aagttcactc catattgggg cttctccacc caaaattctt
    11821 gctttttctt cggctgtaag agtgatgttt tttggcattg gatcattcaa ataatgattt
    11881 tcgacaccca acattaaatc aatataatag ccgttagaca aaaccgtttt gtatccattt
    11941 ttcacagcat ttacaagaga ttgaccagct gcgattcctt cgtttgaacc tttccaagaa
    12001 tgaattatgg catctttaga taggtttttg gtcattattt cttcccaacc catcaatgtt
    12061 ttgttatgtt tttttaacat tggaatcaat tgcatagtga aataggtttg cagttcgtga
    12121 ttgtttgcaa aattattttt ctttttaaat tcctgaattt ttggattcga atcccaatct
    12181 tttccttcgt tttcgtctcc gccaatatga aaataattcc ctgaaaacaa cggacaaacc
    12241 tcgtcgaaaa tttcactcaa caattgatac gtttttggat tggatgggtc taatgttgga
    12301 gtgaaaattc ctgaatttct ctctagagaa taggtgtttt tgtcaaaatt gagtttgctt
    12361 cccacttcag gataagcagt caggatggct gttccgtgac cagggacatc aatttctggg
    12421 atcaccataa tgcctctttc atcggcgtat tttactatat tttttatttc ttcttgtgta
    12481 aaataattcc catcagatgc taattgggtt agcttgggat gatttttcaa ttcgattctc
    12541 c
//
LOCUS       DAOSNN010000040        12396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5091_length_12396_cov_4.880399, whole genome shotgun sequence.
ACCESSION   DAOSNN010000040 DAOSNN010000000
VERSION     DAOSNN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12396
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5091_length_12396_cov_4.880399"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLQ70_03545"
     CDS             complement(<1..223)
                     /locus_tag="PLQ70_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQE33720.1"
                     /translation="MKNCLLRQECCGKSTKFKKIDDSIHKEHYNRMHQKLTQNPYYAK
                     KMVRVRSKTVEPVIGTLVNFTNMKRVNTRG"
     gene            complement(282..2354)
                     /locus_tag="PLQ70_03550"
     CDS             complement(282..2354)
                     /locus_tag="PLQ70_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33721.1"
                     /translation="MTNHILIENQYQRTSLFEKENVNYLVRVLKRFNTVPKINNINII
                     TSISQPDIFQIVPNKSIIIGSSFLNKPVLALVYLRYGIEWQLWYKALNSEKKDITLCD
                     IAALEVTRIFYNLLPKDDKEKLENLDYFLITLIKNYKEFNAESLSKQEELQTFHGLNN
                     SNKSFKKSWKSIIENLAKPTEYLLMAGGDLRLNIDEIELLNKYGCRPFPRPDAFTFAS
                     STATSVSNFAFDKTDKTRSILIRNSLKNGFKNATTEFLELLKNNLRKNFKLNDECEII
                     FSPSGTDSSLQIAAITQIISNKEITHVLVASDETGSGVPAALKGCHFENTTALNHPVK
                     KGDPIGGFRTVDLIKIPFRDKNGKLKSAQELDEEVFNAISKTNELGRHVVLHTMDQSK
                     LGYQSPSDELIQKLNKLKKISMQIIVDAAQLRLDPKDLQNYLHKGYIVSITGSKYFTG
                     PPYSGALIIPKSVSKSIQSAKITLPKGLTKYYHHSDWPTSWFCANELSEGFNYGSYMR
                     WNAAIVEMDRYYKTPILYRNMGVEMFCNFVEDSIKDAPFLESISADETKTNSYKSKEF
                     GIRNIRTIFPFFILKNNEVLSVENVKKLYSLLNSDLSNQFEDAPMDIIRLAAQKCHIG
                     QAVEVKYGNSTESAVLRISLGARVISESWVNRDISLFFRNIEAQMNQITIIIKKIELI
                     LSNPELLD"
     gene            complement(2633..3343)
                     /locus_tag="PLQ70_03555"
     CDS             complement(2633..3343)
                     /locus_tag="PLQ70_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQE33722.1"
                     /translation="MGRAFEFRKGRKMKRWSAMAKAFTRIGKDIVMAVKEGGPNPDAN
                     SRLRAVIQNAKAANMPKENVERAIKKATDKDTANYKEVLFEGYAPHGIALLIETATDN
                     NNRTVANVRSYFNKCNGTMGTQGSVEFMFDHTCNFRIPATGIDPEELELELIDFGAEE
                     VFEDEDGILIYAPFNSFGTIQKELESRKIEILSSGFERIPQITKKLTEAEMADVEKLI
                     EKMEEDDDVMNVYHTMEE"
     gene            complement(3406..4440)
                     /locus_tag="PLQ70_03560"
     CDS             complement(3406..4440)
                     /locus_tag="PLQ70_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33723.1"
                     /translation="MKTFLTAFVFFLIATLSNAQEFKTPVDYLNYIGKETDIISKSTW
                     KYTTTVAHTKNARRIDANRKSLIKSIQNATKKIEALKDGYKGDVEYRDQLLAYLSISE
                     KQINQEYEKIIDMQEVAEQSYDEMEAYIMTRDLVNKKINEEIEKLNANQKIFANKYNI
                     QIGEDTSELGKKMKLSNEVFENQTQLYLIFFKVNYTESVLSKAIESNDISAIQQNSNA
                     LEQYSNEGMEKLKTFKPFKNDMSLVLATKKVLEFTKKEALELAPTTISFMMLNQKFQE
                     SKKVMDNKSASSRTKEEVDNFNKLVNEVNNEVGNYNKTINKFNVDRSNAINNWNITSD
                     NFIAKYVPID"
     gene            complement(4447..6489)
                     /locus_tag="PLQ70_03565"
     CDS             complement(4447..6489)
                     /locus_tag="PLQ70_03565"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HQE33724.1"
                     /translation="MKKISLLLLLTTFSLLGQNSEKTCEMLSKINFLIQNEHYHPKPV
                     DDSLSVYVFDTFIDALDDNRNLFTKSEYEKLCKHRLKIDNYILQKDCSFMNDFVSIYQ
                     LALERKKKVLQKIQIEVFNYNGKDTVKFSKKNFPFDLTITDFDKVWKKRMKYDILEDI
                     SKLSSNLDSLKLNFTSLEKTTKTKIFESNLCKINNILGDKKGMEYDLQNTFLNIFCTY
                     FDPHTNFFSQDAKSSFMSSLSTSGLSLGLNVTLNEKEEIIVDEIVPGGPAARTNKFEK
                     GDVVLKISNNKGEDYWVSCTSLDKIGELIFSDSNEVIELTIQKKNGNIFDVLLKKQVM
                     KATENAVSSFIVEKGNTKIGYIEIPNFYSDFDGFSPQGCADDVAKEIIKLKQDNIQGL
                     AINLQDNGGGSMEEAIKLAGMFIDYGPVSVLVNSKNKQTILKDYNRGSAYNGPIVILI
                     NGNSASASEFFSGAMQDYNRALIVGSKSLGKASMQTILPLDNKQQDFVKLTVEKFYRI
                     TGDSNQIKGIIPDIALPVLYDSIVAREISYKTALKYDAIQSKTRFSPFRKDYYPELIE
                     QSNLRSKKNQLFNEITAVNDEVNALYSSSKKPLLVTFEEVFKDVHEIDSLYKKVKKIA
                     STENNCIVSNNSYDSEKLQFDAHQQEINAYKIKALKTNPYIEEVISIVNDFNNFKK"
     gene            complement(6608..7690)
                     /gene="gcvT"
                     /locus_tag="PLQ70_03570"
     CDS             complement(6608..7690)
                     /gene="gcvT"
                     /locus_tag="PLQ70_03570"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQE33725.1"
                     /translation="MKNTALTHIHESLGAKILPFAGYNMPILYEGVNAEHEVVRNGVG
                     VFDVSHMGEFLLTGPNALALIQKVTSNDASTLTIGRVQYSCLPNNDGGIVDDLLVYKM
                     KDEQYLLVVNASNIEKDWNWISSHNDLGVDMKNLSDDYSLLAIQGPKAVEALQSLTSV
                     DLSSIAYYHFEVGDFAGIEHAIISATGYTGSGGFEIYCKNEEVEQVWNKVFEAGATFG
                     IKPIGLAARDTLRLEMGFCLYGNDINDTTSPLEAGLGWITKFNKEFTNSENLKKQKEA
                     GVSKKLVAFEMQERAVPRHDYEIVNASGEVIGIVTSGTMSPSMNVGIGMGYVTTENSK
                     IDSEIFIRIRKNDVPAKVVKLPFYKK"
     gene            complement(7788..8060)
                     /locus_tag="PLQ70_03575"
     CDS             complement(7788..8060)
                     /locus_tag="PLQ70_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33726.1"
                     /translation="MKTDSSLLLYNKYYSLTNKEIEVELKNKTKWRGKFLGYFRGEKK
                     YISKWQLVDIDVLFGSDNFGFLMGRIIVHKDIVKIFFFQDNSIMML"
     gene            complement(8066..10039)
                     /locus_tag="PLQ70_03580"
     CDS             complement(8066..10039)
                     /locus_tag="PLQ70_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HQE33727.1"
                     /translation="MENSKNGIKSKVTIASLLVALGIIYGDIGTSPLYVFKAIIGTRE
                     INMMLVYGGVSCVFWTLVFQTTIKYIWLTLRADNHGEGGIFSLYALVRRYGKKLVIPT
                     ILGATTLLADGIITPPISVSSAIEGLSMVKGLEDIIIPGNLLTIGIVVGILSLLFFFQ
                     RFGTQVIGKTFGPIMSIWFTMLVVFGVRQILIHPNILLALNPYFAYDLLANYPNGFWL
                     LGAVFLCTTGAEALYSDLGHCGIKNIRITWIYVKISLITAYLGQAAWLMHQGQPLLNG
                     RNPFFEIIPNWFLLPSVIIATSATIIASQALISGSFTLISEAMNLNFWPRVTVKQPSD
                     SKGQIYIPSINSLLWFGCVLMMVYFKESTHMEAAYGFSITITMMMTSVLLGYFLYFRL
                     KWNKVYVVLFLTVFGAIEIAFFIANVAKIKERWMFLFFELFIFIVMYVWYFARKINNR
                     YTKFIELGKYSEQLTELSQDFSIPKFATHLVYLTKADRRHEIEEKIIQSIFSKKPKRG
                     DVYWFLHINRTEDPFTLSYNVSELVDDKVIKVNINIGFRIQPRTELYFKKIVQDLVAN
                     KELNLHIRPDGSTKYNNEPDFRFVVFKKFLSVENEFTIRDGLLLKGYFMLKLLGLSDE
                     RAFGLDKSDVEVEQIPLVYQPITNIQLERELKN"
     gene            complement(10128..10814)
                     /locus_tag="PLQ70_03585"
     CDS             complement(10128..10814)
                     /locus_tag="PLQ70_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQE33728.1"
                     /translation="MNKAEILIIDDEAPIRKLLEIALESNDYKVWQAETGKAGVLMAV
                     NHPPELILLDIGLPDKSGHEILKELRSWYTKAIIILSVQNSEEDIVTALDNGATDYLT
                     KPFRTAELLARIRSVIKRNQRNEESTVSNFGNLQIDFSARMVKRNNEVLKLTSTEFNL
                     LSLFMKNEGRVLTHQYILKEIWGVGYQTETQYLRVFVGTLRKKIEDNPNQPKYIVTES
                     GVGYRFSSSL"
     gene            complement(10807..11889)
                     /locus_tag="PLQ70_03590"
     CDS             complement(10807..11889)
                     /locus_tag="PLQ70_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQE33729.1"
                     /translation="MTNLETKKYSIKQQCGISIVAVLLIATVCFYASKFIDYRITALI
                     LLMTVSIVAIVFDIIPVLITAILSGLLLNFFFIQPLFTFHITDTEDILLFLMYLIIAL
                     VNAVLTFKIRQVEKKARDKEEKEKTIKLYNTLLNSLSHELRTPIATLIGAVDILKENN
                     QTLSKKNQEDLLSEIDKASIRLNRQVENLLNMSRLETGMLLIKQDWCDVNELTNGVVQ
                     KFSSLNTNHIIDFQLNESLPLFKLDSGLIAEVLRNLIHNAVLYTPEKTTIKIEVLQES
                     SNCIIVISDNGNGFPESEIQFAFDKFYRLPHTKTGGSGLGLSIVKGFVEAHNGTITLE
                     NNSISGAKFTITIPTETSYLKNLKNE"
     gene            complement(12016..>12396)
                     /locus_tag="PLQ70_03595"
     CDS             complement(12016..>12396)
                     /locus_tag="PLQ70_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQE33730.1"
                     /translation="VGNPSNFIRIQELYNNDLELFKKDFSSFSYTDEETLEAMKSIYK
                     TDGYIAEPHGAVGYLGLKKELINHPNAIGIFLETAHPIKFLDVVEPALNITLPIPTQI
                     ESVLNKEKVSTKIKTYEELKAFLG"
BASE COUNT         3816 a         2160 c         1917 g         4503 t
ORIGIN      
        1 ttcctctagt atttactcgc ttcatattgg taaaattgac caatgttcca attacaggtt
       61 ctactgtttt actccttact cgtaccattt tcttggcata gtatgggttt tgagtgagtt
      121 tttggtgcat tctgttgtag tgttctttat ggatgctgtc gtctatcttt ttaaacttag
      181 tgctttttcc gcagcattct tgccgtaaga gacagttttt catcatagaa ataatctcca
      241 aaagaaaatg gaactattta atacaactgc gaacttctat tttaatctaa caattcagga
      301 ttactgagaa ttaattctat ctttttaata ataatcgtaa tctgattcat ttgtgcttca
      361 atattcctaa aaaacaagct aatatcacgg ttaacccaac tttcagaaat aactcttgca
      421 cctaaactaa ttcttaaaac agcactttca gttgaattgc cgtattttac ttcaacagct
      481 tgtcctatgt gacatttctg agcagctagt ctaataatat ccataggagc atcttcaaat
      541 tgattggata aatcagaatt caacaatgaa tacagttttt tcacattttc aacagataat
      601 acttcattat ttttaaggat aaagaaaggg aaaatggttc taatatttct tattccaaac
      661 tctttacttt tataactatt tgtttttgtt tcatcagcag aaattgactc taaaaatggg
      721 gcatctttta ttgaatcttc aacaaaattg cagaacattt cgacacccat atttcggtat
      781 aaaatggggg ttttataata gcgatccatt tcaacaatag ccgcattcca acgcatatag
      841 gaaccataat taaatccttc tgacaattca tttgcacaaa accaggaagt gggccaatca
      901 gaatgatggt aatatttagt caaccccttt ggcaaagtga tttttgcaga ttgtattgat
      961 ttgctaacgc ttttgggtat aattaaagct cctgaatatg gaggtcctgt gaaatatttg
     1021 cttcctgtta tagaaactat atatccttta tgcaaataat tttgtaggtc tttggggtct
     1081 aatcgtagtt gtgctgcatc aacaatgatt tgcattgata tttttttgag cttgttcagt
     1141 ttttgaatca attcgtcact tggggattga tagcctaatt tggattgatc cattgtatgc
     1201 aaaaccacgt gccttcctaa ttcattcgtt tttgaaatgg cattaaatac ttcttcgtct
     1261 aattcttgag ctgattttaa tttaccattt ttatctctga aaggaatttt aatcaaatca
     1321 acggttctaa accctcctat tggatctcct ttttttacgg gatggtttaa tgctgttgtg
     1381 ttttcgaaat ggcagccttt tagcgctgca ggtactccgc ttcctgtttc gtcagaggca
     1441 actaaaacat gggtaatttc tttatttgaa ataatttggg ttatagctgc aatttgaagt
     1501 gaggaatcgg ttcctgatgg cgaaaaaatt atttcacatt cgtcgttcag cttaaaattt
     1561 ttcctgagat tgttttttag taattccaaa aactcagtag tggcattttt aaagccattt
     1621 ttcaggctgt ttctaatcaa tatactccta gtcttgtcgg ttttatcaaa agcaaaatta
     1681 gaaacacttg tagccgttga ggacgcaaaa gtaaaggcat ctggtctagg aaatggtcgg
     1741 caaccgtatt tgtttaacaa ttcgatttcg tcaatattta atctcaaatc accgcctgcc
     1801 atcaacaaat attctgttgg ttttgccaga ttttcaataa tggatttcca agatttctta
     1861 aagcttttat tagaattatt caatccgtga aaagtttgca attcttcttg ttttgacaaa
     1921 gattcagcat taaactcttt atagttttta atcaaagtaa ttaaaaaata gtccaaattt
     1981 tctaattttt ctttatcatc tttgggtaat aaattataaa atattcgagt cacttcaagt
     2041 gctgcaatat cacacaatgt aatgtctttt ttctcgctat ttaaggcttt ataccatagt
     2101 tgccattcga taccatatct taaataaacc aaggctaaaa caggtttatt caaaaacgaa
     2161 gaccctataa taattgattt attaggtacg atttgaaata tatcaggctg actaattgaa
     2221 gttattatat tgatgttgtt aattttcgga actgtattaa accgctttaa aacacgaacc
     2281 agataattca cattttcttt ttcaaataaa ctggttcttt ggtattgatt ttcaataagt
     2341 atatggtttg tcatagatgt atttattttg aaatagccaa caattcctag aaaaaacaaa
     2401 tgaggacttt tgttttttct acattttagt atgttgcaat tgtaaaattt aaaacaaata
     2461 taatgaactc gtgttcataa taaaagaatt gttttattta aaaaacggcc tttcctaagt
     2521 agagaatagt tgcaaggcct ctattgttta gaatcaaatg attttttatc atttgaaaat
     2581 ttagaaataa aaaaatccct ttctaaaagg gattttaatg ataaattctt aattattctt
     2641 ccatagtatg atagacgttc atcacatcat catcttcttc cattttttcg attaatttct
     2701 cgacgtctgc catttcggct tcggtcaatt tttttgtgat ttgaggaatg cgctcaaaac
     2761 ctgaagaaag gatttctatt tttctgctct ctaattcttt ttgaatggtt ccaaaactat
     2821 tgaaaggcgc atagattaag attccgtctt catcttcaaa aacttcctcg gcaccaaaat
     2881 ctattaattc taactctaat tcttcgggat caattccggt tgctggaatt ctaaagttac
     2941 acgtatggtc aaacataaat tcaaccgaac cttgtgttcc catcgttccg ttgcatttgt
     3001 taaaatagct acgaacattg gcaacagttc tattattgtt atcggttgcg gtttcgatta
     3061 acaaagcgat accgtggggt gcatatcctt cgaataaaac ctctttataa ttggcagtat
     3121 ctttatcggt tgcttttttg atggcacgtt ctacattttc tttaggcata ttggcagcct
     3181 tggcattttg tatcactgct cttaatctcg agttggcgtc aggatttggt ccaccttcct
     3241 taacggccat cacaatatct tttccaattc tggtaaatgc tttggccata gctgaccaac
     3301 gtttcatttt tcttcctttt ctgaactcaa acgctcttcc catttctaat ttttattttg
     3361 aactgcaaaa gtacagttat tagttatttt ttcaaatatt tttttttaat caattggcac
     3421 atatttagca ataaaattat cgcttgtaat gttccaatta ttaattgcat ttgaacggtc
     3481 aacgttgaat ttattgatgg ttttattgta gtttcctact tcattattca cttcgtttac
     3541 caatttatta aaattatcaa cttcttcttt agtcctgctg ctggccgatt tgttgtccat
     3601 tactttttta ctttcttgaa atttttgatt caacatcata aacgaaatag ttgtcggagc
     3661 caattcaagg gcttcttttt ttgtaaattc taaaactttt tttgtggcta aaaccaatga
     3721 catatcgttt ttaaaaggct tgaaagtttt gagtttttcc attccttcat ttgaatattg
     3781 ttctaatgca ttgctgtttt gctgaatggc actaatatca ttgctttcaa tagctttgga
     3841 caaaaccgat tctgtataat ttactttaaa aaagataaga tacaattggg tttgattttc
     3901 gaaaacttca ttggataatt tcattttttt gcccaattcg ctagtatctt cgcctatttg
     3961 gatgttgtat ttattggcaa atattttttg gttcgcattc agtttttcaa tttcttcatt
     4021 aattttttta ttaactaaat ctctcgtcat aatataagct tccatttcat cataggattg
     4081 ctcggctact tcctgcatat ctataatttt ttcatattct tgattgattt gtttttcgga
     4141 aatagataaa taagcaagca gttggtctct atactccaca tcacccttat atccgtcttt
     4201 caatgcttct atttttttag tagcattttg aatacttttt atcaaagatt ttcgattggc
     4261 atctattctt cttgcatttt ttgtatgagc tacagttgtg gtatatttcc aagtcgattt
     4321 agaaataata tcggtttctt tgcctatata gttcaaataa tcaacagggg ttttaaattc
     4381 ttgggcattt gaaagggttg cgattaagaa aaatacaaat gcagttagga acgttttcat
     4441 taaaggttat tttttaaaat tattgaaatc atttactata gaaatgactt cttcgatata
     4501 gggattggtt tttagagctt ttattttata ggcattaatt tcctgctgat gagcatcaaa
     4561 ttgtagcttt tctgaatcgt aagaattgtt cgaaacgata caattatttt cggtcgaagc
     4621 aatttttttc acttttttgt aaagagaatc tatttcgtga acatctttaa atacctcttc
     4681 aaaagtaacc aagaggggct ttttcgaact actatacaat gcgtttactt catcgtttac
     4741 tgcagtaatt tcattgaata gctgattttt tttcgacctt aaattacttt gttcgatcaa
     4801 ttctggataa taatctttgc gaaagggaga aaatcttgtt tttgattgaa ttgcatcata
     4861 tttcaaagcg gttttgtaac ttatttcacg agctacaatg ctgtcgtata aaacaggtaa
     4921 agcaatgtca ggaataattc ccttgatttg attactatca ccagtaattc ggtaaaactt
     4981 ctccacggtt agtttaacaa agtcctgttg cttattatcc aaaggcaaaa tagtttgcat
     5041 cgaggctttg cccaatgatt tacttccaac aattaatgct cgattataat cttgcatagc
     5101 acctgaaaag aactcacttg ccgaagccga atttccgttg attaaaatga ctattggccc
     5161 attataggca cttcctcgat tgtaatcttt tagaatagtt tgcttgtttt tactatttac
     5221 caaaaccgaa acgggaccat aatcgataaa cattccagcc agttttatag cttcttccat
     5281 agaacctccc ccattatctt gaaggttaat ggctaatccc tgaatgttgt cttgtttgag
     5341 tttgataatt tctttagcca catcatctgc acagccttga gggctgaatc catcaaaatc
     5401 ggaataaaaa ttagggattt cgatatagcc aattttagta ttcccttttt cgacaataaa
     5461 gctggaaacc gcattttcag tggctttcat tacttgtttt ttgagtaaaa catcaaaaat
     5521 atttccgttt ttcttctgga tagtcaattc aataacttca ttggaatctg aaaaaattaa
     5581 ttctcctatt ttatctaatg aagtgcagga aacccaataa tcttctcctt tattgttaga
     5641 aatttttaaa acgacatcgc ctttttcaaa tttgtttgtt cttgcagcgg gacctcctgg
     5701 aacaatttcg tcaacaataa tttcttcttt ttcgtttaga gttacattga gccctaaaga
     5761 caaaccgctt gtggataggc ttgacataaa actggattta gcatcctgtg aaaagaaatt
     5821 ggtgtgcggg tcaaaatagg tgcaaaaaat atttaagaat gtattttgaa ggtcatattc
     5881 catgcctttt ttgtccccca aaatattatt tattttgcat aaattagact caaatatttt
     5941 agtttttgtt gttttttcga gtgaagtgaa attcagttta agtgaatcta aattagagct
     6001 taatttagaa atgtcttcca gaatatcgta tttcattctt tttttccaaa ccttgtcgaa
     6061 atcggttatg gttaaatcaa acggaaagtt tttctttgaa aatttaacag tgtctttgcc
     6121 attgtaattg aatacttcaa tttgtatttt ttggagtact tttttctttc tttctaaggc
     6181 taattgatag atcgaaacaa agtcattcat aaaagaacaa tccttttgca aaatgtaatt
     6241 gtctattttt aatcggtgtt tacagagttt ttcgtattct gatttggtaa ataaatttct
     6301 attatcatcc aaagcatcga taaaagtatc aaaaacataa accgataaac tatcatcaac
     6361 tggttttgga tgataatgtt cgttttgaat taagaaatta attttggaaa gcatttcaca
     6421 tgtcttctca ctattttgac ccaatagcga aaatgttgtc aataatagta ggagtgaaat
     6481 tttcttcatt tgtgagttta taggtgcaat ttaaaataaa attaccgcaa attcacaaat
     6541 tatttcacat ttttgaaatg cttaatttgt gaatttgcgg ttttagcttt ttgaaaaatc
     6601 ggtactctta ttttttataa aaaggcaatt ttactacttt ggcaggaaca tcattttttc
     6661 taattctaat gaaaatttca ctgtctattt tactattttc agtggttaca tatcccattc
     6721 caattccaac attcattgaa ggtgacatcg ttccagaagt tacaattcca ataacctcgc
     6781 ccgatgcatt tacaatttca taatcgtgtc ttggtacagc gcgttcttgc atttcgaaag
     6841 ctactaattt cttagaaact cccgcttctt tttgtttttt caggttttca gaattagtaa
     6901 attctttatt gaatttggta atccaaccca aaccagcttc taatggtgaa gtcgtgtcat
     6961 tgatgtcgtt tccgtacaaa cagaatccca tttccaatcg caacgtatca cgagcagcca
     7021 aaccaatagg tttgattcca aaggtagcac cagcttcaaa aactttgttc caaacttgtt
     7081 ctacctcttc atttttacaa taaatctcga aacctcctga acctgtatag cctgttgccg
     7141 aaataattgc gtgttcaatt cctgcaaaat cgcctacttc aaaatgataa taagctatgg
     7201 aagacaaatc gaccgaagtc aaagattgta gtgcttctac cgcttttggc ccttgaatgg
     7261 ccaatagtga ataatcgtca gaaaggtttt tcatatccac acccaaatcg ttgtgagacg
     7321 aaatccaatt ccaatctttc tcaatatttg aagcattcac aaccaataaa tactgctcgt
     7381 ctttcatttt gtacaccaat aaatcatcta caattccacc gtcattgttc ggtaaacaag
     7441 aatattgcac acgtccgatg gttaaagtcg atgcatcatt cgaagttact ttttgtatta
     7501 atgccaaagc attgggtcct gtcaacaaga attcgcccat atgcgaaacg tcaaaaaccc
     7561 ccacaccatt tcgaaccacc tcgtgttccg cattcactcc ttcgtataaa ataggcatat
     7621 tgtaaccagc aaacggaagt attttggctc ccaaactctc gtgtatgtgc gtaagcgcag
     7681 tatttttcat cgtagattta tatataattt gaagcggcta atttattcat tttttatgga
     7741 gtgacaaagc gttttaaaag gcattttgac actccttttg tacaagttta aagcatcatt
     7801 atagagttgt cttggaaaaa aaatattttt acaatatctt tatgaactat tattcttccc
     7861 attaaaaatc caaaattatc agaaccaaat aacacatcaa tatcgactaa ttgccatttt
     7921 gaaatatatt ttttttctcc tctaaaataa cccaaaaatt tacctctcca tttggttttg
     7981 ttttttagtt ctacttcaat ttctttattg gttaaactat aatatttgtt gtaaagcaat
     8041 agagacgaat ctgttttcat tgctattaat tttttaattc ccgttctaat tgaatgttgg
     8101 tgatgggttg atagaccaaa ggaatttgtt ctacttcaac gtcgcttttg tccaatccga
     8161 aggctctttc gtcactcaaa ccaagaagtt ttagcataaa atagcctttc aataacaagc
     8221 catcacgaat agtaaattca ttttcgacag acaagaattt tttgaaaact acaaatctaa
     8281 aatcgggttc attgttgtat tttgtggaac catctggcct gatgtgtaaa ttaagttctt
     8341 tattggctac caaatcttgc acgatttttt tgaaatacaa ttcggttcta ggttgaattc
     8401 taaaaccaat attgatgttc acttttatta ctttatcgtc cactaattcc gagacattat
     8461 acgaaagtgt aaaaggatct tcggttcgat tgatgtgcaa aaaccaatag acatcgccac
     8521 gtttaggctt tttagaaaaa atagattgaa tgattttttc ttcgatttcg tgtcttctat
     8581 cggctttggt taaatacacc aaatgtgtag caaattttgg aatagaaaaa tcttgactta
     8641 attcggtcaa ttgttcgctg tattttccaa gctcaataaa tttagtatat ctattgttga
     8701 tttttctagc aaaataccaa acatacatta ctataaaaat gaacaactcg aaaaacaaaa
     8761 acatccatcg ctcttttatt ttagccacat tagcaatgaa aaatgctatt tcaattgcac
     8821 caaaaacagt caaaaacaaa acaacataaa ctttattcca tttcaaccta aagtaaagga
     8881 aatagcctaa caaaacggat gtcatcatca ttgtgattgt aatcgaaaaa ccataagcag
     8941 cttccatatg agtgctttct ttaaagtaaa ccatcatcaa aacgcaacca aaccaaagca
     9001 gtgagttaat gctcggaata taaatttgcc ccttactatc gctgggttgt ttaacggtaa
     9061 ctcttggcca aaaattaagg ttcatagctt cgctaataag ggtaaaacta ccactaatta
     9121 aagcttgtga ggcaataatc gtggctgatg ttgctatgat tacactaggt agtaaaaacc
     9181 aattgggaat aatctcaaaa aaaggatttc ttccattcaa tagcggttgt ccctgatgca
     9241 ttaaccaagc tgcttgacct aaataggccg ttattaaact tatttttaca taaatccaag
     9301 tgatgcgaat attcttgatg ccgcagtgtc ctaaatctga atacaaagct tcggctccag
     9361 ttgtacaaag aaaaacggct cccaaaagcc aaaaaccatt agggtaattg gctaataaat
     9421 cataggcaaa ataaggattt aaagctaaaa gaatattagg atgaattaga atttgtcgaa
     9481 ctccaaaaac cactaacatc gtaaaccaaa tggacattat aggaccaaat gtttttccaa
     9541 ttacttgtgt gccaaaacgt tgaaagaaaa ataataaaga caagatacca accacaattc
     9601 caatggttag tagatttcct ggaataataa tatcttccaa tcctttgacc atactcaaac
     9661 cttcaatcgc cgaagaaact gatatgggag gtgtaattat tccatcggcc aacagggttg
     9721 ttgctccaag aattgtaggg attaccagtt ttttgccata cctacgaact aaagcataaa
     9781 gtgaaaaaat gccgccttcg ccgtgattat ctgcccgaag tgtgagccaa atatacttga
     9841 ttgtggtttg aaatactaaa gtccaaaaca cacaggaaac cccgccataa accaacatca
     9901 tattgatttc tcgtgttcca attattgctt taaaaacata gagtggactc gttccaatat
     9961 ctccataaat aattccaaga gccactagaa gtgaagcaat tgttacttta cttttgattc
    10021 cattttttga attttccatt ttatttattt gctaaatttt aatggtacaa agttcaatca
    10081 aatggtattg gtgaaatata aagatgtatt tggataatat aaagatttta taaagacgaa
    10141 ctaaaacgat aacctacgcc actttcggtg acaatatatt tgggttggtt gggattgtct
    10201 tctatctttt ttcgtaaggt tcccacaaaa actctcaagt attgggtttc ggtttggtag
    10261 cctactcccc aaatttcttt taaaatgtat tgatgggtca acactcttcc ttcatttttc
    10321 ataaaaagag aaagtaagtt gaattcggtt gaagtaagtt ttaaaacttc gttattgcgt
    10381 ttgaccattc gagcgctaaa atcgatttgt aaattaccaa aatttgaaac tgtactttct
    10441 tcatttcttt ggttgcgttt tatcaccgat ctaattcttg ccaataattc ggcagttctg
    10501 aaaggttttg tcaaataatc ggttgctccg ttatctaagg cggttacaat atcttcttca
    10561 ctattttgaa ccgaaagaat gataatggct ttggtgtacc aagatcgtaa ttcttttaaa
    10621 atttcgtgac cacttttgtc gggtaaacca atatcaagca agattaattc gggtggatga
    10681 ttgactgcca taagaacgcc agcttttcca gtttcggctt gccagacttt gtaatcgttg
    10741 ctttccaagg caatttctag caattttcga attggtgctt catcgtcgat aattagtatt
    10801 tcagctttat tcattcttta aattttttaa ataagaagtt tcagttggaa tcgttatggt
    10861 aaatttagct ccgctgattg agttgttttc taaagttatg gttccattat gagcctcgac
    10921 aaatcctttt acaatggata aacccagtcc gctgccgcct gttttggtat gcggtaatcg
    10981 gtaaaattta tcaaacgcaa actgtatttc gctttctgga aaaccatttc cgttatctga
    11041 aataacaata atacaattgg aagattcctg taaaacttct attttaattg tcgttttttc
    11101 gggcgtataa agcaccgcat tatgtattaa attgcgcaac acttcagcta tcaaacctga
    11161 atctaactta aaaagaggca agctctcatt gagttgaaaa tcaataatat gattggtatt
    11221 caaagaggag aatttttgaa ccacgccatt cgtcagttca ttaacatcgc accaatcttg
    11281 ttttataagt agcattccag tttctaaccg actcatattt aataagtttt ccacttgtcg
    11341 attaagtcta atgctggctt tgtcgatttc agaaagtaaa tcttcttgat tttttttaga
    11401 aagagtttga ttgttttctt ttaaaatatc aaccgcccca attagggttg caatgggagt
    11461 tcttaattcg tgcgaaagcg aatttaatag cgtgttgtag agttttatgg tcttttcttt
    11521 ttcttctttg tctcttgctt ttttttcgac ttgtctgatt ttgaacgtta aaaccgcatt
    11581 gactaaggca ataatcaagt acatcaaaaa caacaaaata tcttcggtat cggtgatgtg
    11641 aaaggtgaag agtggttgaa taaaaaagaa attcaatagc aaaccactta aaatagctgt
    11701 aattaatacg ggtataatat caaaaactat tgccacaatt gaaactgtca ttaataaaat
    11761 taaagcagtt attcggtaat caataaattt tgaagcataa aaacaaactg tcgcaataag
    11821 tagaacagcc acgatactaa taccgcactg ttgttttatt gagtatttct ttgtttcaag
    11881 attggtcaca atacatttat ttttattttg aacaaatgta attcaattct atatattttt
    11941 gatgtacaaa aaaaccgcag aatcattaga tataaatctg tgaatctgcg gcttatttat
    12001 ttaagtgaaa agtgtttacc ccaaaaacgc tttcaattcc tcgtaagtct tgatttttgt
    12061 gcttactttt tctttgttca gcacactttc tatttgtgtt ggaattggaa gtgttatatt
    12121 taatgctggt tcaaccacat ctaagaattt aataggatga gccgtttcca agaaaatacc
    12181 aatagcgttt ggatggttta tcaactcctt tttcaaacct aaatatccaa cggctccgtg
    12241 aggttctgca atgtagccat cggttttata gatggatttc attgcttcta aagtttcttc
    12301 atcggtataa gaaaacgaag aaaaatcttt tttgaatagt tccaaatcgt tgttgtacaa
    12361 ttcctgaata cgaataaaat tacttggatt tcctac
//
LOCUS       DAOSNN010000041        12382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5098_length_12382_cov_3.844082, whole genome shotgun sequence.
ACCESSION   DAOSNN010000041 DAOSNN010000000
VERSION     DAOSNN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12382
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5098_length_12382_cov_3.844082"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="PLQ70_03600"
     CDS             complement(<1..1218)
                     /locus_tag="PLQ70_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HQE33731.1"
                     /translation="MGFLEEIQRRRTFGIISHPDAGKTTLTEKLLLFGGAIQEAGAVK
                     NNKIKKGATSDFMEIERQRGISVSTSVLAFNYQDKKINILDTPGHKDFAEDTFRTLTA
                     VDSVIVVIDVAKGVEEQTEKLVAVCRLRNIPIIVFINKLDREGKDAFDLMDEVEQKLG
                     LTVTPLSFPIGMGYDFQGIYNLWEENINLFSGDSRKNIEETIAFSDVKSPELEKIIGE
                     KPAHKLREELELIDEVYPKFDRQEYLDGKLQPVFFGSALNNFGVRELLDCFVQIAPSP
                     RAKDSETRLVDPKEEKMSGFVFKIHANMDPKHRDRLAFIKIVSGTFERNKPYYHVRQK
                     KNLKFSSPNAFFAEKKEIVDISYPGDIVGLHDTGNFKIGDTLTEGEIMSFKGIPSFSP
                     EHFRYINNADPMKA"
     gene            1515..2807
                     /gene="eno"
                     /locus_tag="PLQ70_03605"
     CDS             1515..2807
                     /gene="eno"
                     /locus_tag="PLQ70_03605"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQE33732.1"
                     /translation="MSIIVKIHARQIFDSRGNPTVEVDVVTDNGILGRAAVPSGASTG
                     KFEAVELRDGGKNFLGRGVLKAVENVNTIIAEELVGTSVFEQNLIDQTMIELDGTPNK
                     AKLGANAILGVSLAVAKAAANELGMPLYRYVGGVSANTLPVPMMNIINGGSHSDAPIA
                     FQEFMIFPVKATSFTHALQMGTEIFHNLKKVLHDRGLSTAVGDEGGFAPNLAGGTEDA
                     LDTIKLAVEKAGYTFGDEVMIALDCASSEFYENGVYDYTKFEGPTGKIRTSAEQVEYM
                     AELVAKYPIISIEDGMDENDWDGWKMLTEKIGDKVQLVGDDLFVTNVERLSTGIEKGI
                     ANSILVKVNQIGSLTETIAAVNMAKNAGYTSVMSHRSGETEDNTIADLAVALNCGQIK
                     TGSASRSDRMAKYNQLIRIEEELANTAYFPGKNAFKVK"
     gene            2957..4240
                     /locus_tag="PLQ70_03610"
     CDS             2957..4240
                     /locus_tag="PLQ70_03610"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HQE33733.1"
                     /translation="MSKTAKLEIDGKIYELPIFVGTENEMSIDISKLRDQSGAVTLDQ
                     GYKNTGACKSEITFLDGEEGVLHYRGYSIEELAEKAHFLEVCYLLIFGELPTAAQLLE
                     FETHIRRYTLVNEEMKSIIDGFPKTAHPMGVLSALTSALIAFNPSAVNPEHPDELYEA
                     VCKLMGKFLVIASWTYRKSQGFPLNYYDNTIGYVENFMQLMFKLPTEPYKANPVIVQA
                     LDQLFILHADHEQNCSTSTVRMVGSSHAGLFASISAGVSALWGPLHGGANQAVLEMLE
                     EINAHGGDADKYLAKAKDKDDPFRLMGFGHRVYKNFDPRAKIIKEAADEVLKTIGFED
                     PLLEIAKKLEAAALTDEYFIARKLYPNVDFYSGIIYRALGIPTDMFTVMFAIGRLPGW
                     IAQWKEMRLNKEPIGRPRQVYTGSPLRSFVEMDKR"
     gene            4451..5365
                     /locus_tag="PLQ70_03615"
     CDS             4451..5365
                     /locus_tag="PLQ70_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HQE33734.1"
                     /translation="MLKLSINDETSRLRTVVLGVANSNGPTPSIEEAYDPKSLEHILA
                     GTYPKEEDMVVEMEAFNQIFEKYEVTVFRPEIIENYNQIFTRDIGFVIGDVFIKSNIL
                     PERVRELDAIQYVIDQIQPEKVVTPPEEVHIEGGDVMLWDDYVFIGTYKGSDYKDYIT
                     ARTNREGVQFIKELFPNKKVKEFDLIKSKIEARDNALHLDCCFQPVGKDKAIIYKRGF
                     REEADYLFLVQLFGEENLFHITRKEMYSMCSNVFSIDTNVVVSEKKFSRLNKWLKKQG
                     FIVEKIPYAEISKQEGLLRCSTLPLMRD"
     gene            5663..6598
                     /locus_tag="PLQ70_03620"
     CDS             5663..6598
                     /locus_tag="PLQ70_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HQE33735.1"
                     /translation="MNQTTNSILMIRPVAFRMNEQTAVNNYYQKVLDGLSKETVNAKA
                     QEEFDGLVQKLKMVGVDVIVVEDTLNPDTPDSIFPNNWISFHESGDVVLYPMFAKNRR
                     EERRDDILDIVEDQGFVINEIMDYTSAEEDGFYLEGTGSVVLDRENGKAYCALSPRAD
                     EELFIEFCEDFEYSPVIFEAFQTVDGERKLIYHTNVMMCIGDTFAVICADCIDDKKER
                     KMVLDSLRGDDKEVILITEAQVNSFAGNMLEVKGADDRRYLVMSSSAYQSLTKKQIAQ
                     IEEHVTILSSNLDTIEACGGGSARCMMAEIFLPRG"
     gene            complement(6666..7295)
                     /locus_tag="PLQ70_03625"
     CDS             complement(6666..7295)
                     /locus_tag="PLQ70_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HQE33736.1"
                     /translation="MDLFTYLFAALFSVLNPIGTVPIFVGLTHSDSKKERSRISLWTA
                     INVFIILIVSFFIGQYVLTFFGISIEALRIAGGIIIVSSGFSLLSGKFTKKRGINKKA
                     ESEAQKRNDIALTPLAIPMLAGPGSISLLIAFYQEHHKPNEIMISCLAIFAIAIAIFV
                     ILRSAHYLSKILGASGIVAISRIVGFIVIAIGIQYIVSALLTIIKGNLL"
     gene            7634..7999
                     /locus_tag="PLQ70_03630"
     CDS             7634..7999
                     /locus_tag="PLQ70_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQE33737.1"
                     /translation="MKNKKTLVLGASAKPEKYSFKAITLLVQKGHSVLAIGQNAGEVA
                     GIKIQTKAIPLKNIDTISLYLNPIRQRDYYNYIVESKPKRVIFNPGTENPEFYQLLEL
                     NGIKAEVACTLVLLTINQY"
     gene            8534..9484
                     /locus_tag="PLQ70_03635"
     CDS             8534..9484
                     /locus_tag="PLQ70_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HQE33738.1"
                     /translation="MKKDAKIYIAGHRGMVGSAICRTLSAKGYTNLIGASSANLDLRN
                     QDAVRDFISKEKPDVVIDAAAKVGGILANNNYPYQFIMENMQIQNNLIDSALQSGIEK
                     FIFLGSSCIYPRLAPQPLKEDYLLTSDLEPTNEWYAIAKITGVKACQAIRKQFGKDYV
                     SLMPTNLYGTYDNFDLNTSHVLPAMMRKFHEAKENNHAPVTLWGSGTPLREFLFVDDM
                     AQAVVFALENTLPDYLYNIGTGVDLTIKELAETIQKITGHQGEIIWDSSKPDGTPRKL
                     MDISKMHDLGWKHKVDLEEGIQLTYDWFLEHVNDFKQVKM"
     gene            9618..10709
                     /gene="gmd"
                     /locus_tag="PLQ70_03640"
     CDS             9618..10709
                     /gene="gmd"
                     /locus_tag="PLQ70_03640"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQE33739.1"
                     /translation="MDTKQKVALITGITGQDGSYLAELLLEKGYHVHGVKRRASSFNT
                     QRIDHIYQDQHENHVNFKLHYGDLTDSTNIIRIIQEVQPDEIYNLGAMSHVKVSFDSP
                     EYVANVDGIGTLRILEAVRILGLEKKTRIYQASTSELYGGLAENKNAAGFYDENSPFY
                     PRSPYGVAKIYGFWITKNYREAYDIYACNGILFNHESPRRGETFVTRKITMATAAIAL
                     GKQDCLYLGNLNSQRDWGHAKDYVEAMWRILQQDVAEDYVIATGVTTYIRDFVRFSFA
                     EVGIELAFEGENENETAKIVACNHPEYQLPIGTVVVRVDPQYYRPTEVDLLIGDPSKS
                     KKLGWKPQYDLAGLVKEMVASDLEIAKRV"
     gene            10793..12259
                     /locus_tag="PLQ70_03645"
     CDS             10793..12259
                     /locus_tag="PLQ70_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HQE33740.1"
                     /translation="MKKIGVFISSVQSEFAEERRILCDYMTSDALLGRFFEPFIFENV
                     PAVNNSAISVYSKEVEQCDIYLGIFGKHYGFEDSKGVSPTEREFDLAGLYSKTRLVFL
                     SSDADTDRDSKERILIEKAQQLVVRKSFASPLELKTAVYISLIRYLEEKEYIRTTPFD
                     ATLHSRAVIADLDSQKIAAFVGVAHTKRGFPFSSGSSTETILTHLNLIVQQRITNAAI
                     LLFAKNPQQFFISSEIKCAHFHGTTITKPIPSYHVYKGDVFELVNQAVDFVLSRIDVS
                     VGTREESIQVPIRYELPPAAITEAIVNAVAHRDYSSNGSVQVMLFKDRLEIWNPGSLP
                     YGLTTAKLRNPHSSIPANPLLAEPMYLAGYIERMGTGTGDIIRLCEEAGLEKAPEFLQ
                     EEIFKTILWRKNTTVIENQIVDNVVDNVVDNVVDNVVEKEKQLLELILANKKISAQEM
                     AVHLQLTQRTIQRYLKGLQDENRLERVGSAKGGFWKIK"
BASE COUNT         4126 a         1913 c         2417 g         3926 t
ORIGIN      
        1 ggctttcatc gggtcggcat tgttaatgta tctgaaatgt tctggcgaga aactcggaat
       61 tcctttgaaa ctcattattt cgccttcggt caaagtgtcg ccaatcttga aatttccagt
      121 atcgtgcaat cctacaatat ctcctggata tgaaatatca acgatttctt ttttctcagc
      181 aaagaacgca tttgggctag agaactttaa attctttttc tggcgaacgt gataataagg
      241 tttgtttctt tcgaaagtac ccgaaacaat tttgataaat gccaaacggt ctctgtgttt
      301 tggatccata ttggcgtgga ttttaaatac aaatccagac attttttctt cttttggatc
      361 aactaatctc gtttcagaat ctttagctct tggcgatgga gcaatttgaa caaaacaatc
      421 caaaagttcg cgcacaccaa aattatttaa ggctgaacca aaaaacacag gttgcagttt
      481 tccgtctaaa tattcctgac gatcaaattt aggatagact tcatcaatta attctaattc
      541 ttctcgtagt ttgtgtgcgg gtttctcgcc aataatcttc tctaattctg gactttttac
      601 atccgagaaa gcaatggttt cttcaatgtt tttgcgactg tcaccactaa ataaattgat
      661 gttttcttcc cataagttgt aaattccttg aaaatcatat cccattccaa taggaaaact
      721 caaaggagta actgttaaac ccagtttttg ttccacttcg tccattaggt cgaaagcatc
      781 ttttccttcg cggtctaatt tgttgataaa aacaatgata ggaatatttc tcagtcggca
      841 cacagcgact agtttttcgg tttgttcttc gacacctttg gctacgtcaa tcactacaat
      901 cacactatca actgcagtta gtgttctaaa agtgtcttcg gcaaaatctt tgtgtcccgg
      961 agtatcgaga atgtttattt ttttgtcttg ataattgaat gctaagaccg aggtcgaaac
     1021 cgaaattcct ctctgacgtt caatttccat aaagtcactc gtagctcctt ttttgatttt
     1081 gttgtttttt actgcacccg cttcttgaat cgcacctcca aaaaggagta acttttcggt
     1141 taaggtggtt tttcccgcat cgggatggga aataattcca aaagttcttc ttcgctgtat
     1201 ttcttctaaa aagcccatat ttatttaaat agtttgcaaa agtactattt ataaatgcga
     1261 attcaaaaag caaataaatt tgttagaaat actttatgcg aaaatagata gagaatcaag
     1321 aaaaaggagt ggtgtttatt gactaaacaa ataatatgtt aaacaaaaaa ataacaaaat
     1381 agttcaacct ccttatattt aaaatattat atcgttttcg ttaataacat tacgattttt
     1441 tcatttagtg ttaattgtgg atagtgtata tttgtatcta aataaattat atttcaacat
     1501 taaaattaaa caaaatgagt attattgtca aaattcacgc aagacaaatt ttcgattcaa
     1561 gaggaaatcc tactgtagaa gtagatgtag ttacagataa cggaatatta ggaagagcag
     1621 cagttccatc tggagcttca actggaaagt tcgaagctgt ggaattacgc gacggaggaa
     1681 aaaactttct tggaagaggc gttcttaaag ctgttgaaaa tgtaaatact ataattgctg
     1741 aagaattagt tgggacatca gtttttgaac aaaacttgat tgaccaaact atgattgagt
     1801 tagatggaac tcctaataaa gctaagctag gtgcaaatgc tatacttggt gtttcgcttg
     1861 cagtggctaa agcagcagca aacgaattag gaatgccttt atacagatat gttggtggag
     1921 tttctgcaaa tacattgcca gtgccaatga tgaatatcat caatggtggt tcacactcag
     1981 atgcgccaat cgcatttcaa gagtttatga ttttcccagt aaaagcaact tcttttactc
     2041 acgcattgca aatgggaact gagattttcc ataatcttaa aaaagtattg cacgatagag
     2101 gtttgagtac tgctgtaggt gacgaaggtg gatttgctcc aaacttagct ggtggaactg
     2161 aagatgcttt agatactatt aaattggcag ttgaaaaagc aggttatact tttggagatg
     2221 aagtaatgat tgctttagat tgtgcttctt ctgaattcta cgaaaacggt gtttacgact
     2281 atactaaatt tgaaggacca actgggaaaa tcagaacatc agcagagcaa gtagaatata
     2341 tggctgaatt agtagctaaa tatccaatta tttctattga agatggtatg gatgaaaatg
     2401 actgggatgg ttggaaaatg cttactgaga aaattggaga caaagttcaa ttagtaggtg
     2461 atgatttatt cgttactaat gtggagcgtc tttctactgg aattgaaaaa ggtatagcta
     2521 attcaatttt agtaaaagta aatcaaattg gatctttaac agaaactatt gcagctgtaa
     2581 atatggctaa aaacgcagga tatacttctg taatgtctca ccgttcagga gaaacagaag
     2641 acaatactat tgctgattta gcagtagctt tgaactgtgg acaaataaaa acaggttcgg
     2701 cttcacgttc agaccgtatg gcaaaataca atcagttaat tcgcatcgaa gaagaattag
     2761 cgaatactgc atattttccg ggtaaaaatg cttttaaagt aaaataaagg tgctgatttt
     2821 attaaaaagc catttacgca aagtaaatgg cttttttttt gaattttaac atattcctta
     2881 ggaatttcat tttttagttt gcttgaaatt acctattttt ggaaaataat tttattaaga
     2941 tttattccaa aatactatgt caaaaacagc aaaattagaa attgacggca aaatatatga
     3001 gttacccata tttgttggga ctgaaaatga gatgtccatt gatattagca aattacgtga
     3061 tcaatctgga gcagttactc tagatcaagg gtacaagaat actggtgctt gtaagagtga
     3121 aatcacattt cttgatggag aagaaggagt tttgcattac agaggttatt ccatcgaaga
     3181 attggctgaa aaagctcatt ttcttgaagt atgctatttg ttaatttttg gagaattgcc
     3241 aacggcagcg caattattag aatttgaaac tcacattaga agatatacct tggttaacga
     3301 ggagatgaaa agtatcattg atggatttcc aaaaacggct caccccatgg gagttttgtc
     3361 ggctttgacc agtgctttga ttgcttttaa tccttcggct gtaaatccag aacacccaga
     3421 cgaattgtat gaagcggttt gtaaacttat ggggaaattc cttgtaattg cttcatggac
     3481 ttacagaaaa agtcaagggt ttcctttgaa ttattacgat aatactatag gatacgttga
     3541 aaattttatg caattgatgt tcaaattgcc aacagaacct tataaagcga atccagtaat
     3601 tgttcaagcc ttagatcaat tgtttattct tcacgcggat cacgaacaaa actgttctac
     3661 ttctactgta cgaatggtag gttcctctca cgctggttta tttgcttcca tttccgcagg
     3721 agtttctgcg ctttggggtc cattacacgg tggggcgaat caggccgttc ttgaaatgtt
     3781 ggaagaaatt aacgcccacg gaggagatgc tgataaatat ttggctaaag ctaaagacaa
     3841 agatgatcct ttccgattaa tgggttttgg tcacagagta tataaaaatt tcgatccaag
     3901 agccaaaatc atcaaagaag cggctgacga agttttgaaa actataggtt ttgaggatcc
     3961 acttttggaa attgcaaaaa aattggaagc ggcagcctta actgacgaat attttatcgc
     4021 tagaaaatta tatccaaacg tagatttcta ttcgggtatt atttatcgtg ccttagggat
     4081 tcctactgat atgtttaccg ttatgtttgc catcggtaga ttgccaggtt ggattgcaca
     4141 atggaaagaa atgagactta acaaagagcc tatcggaaga ccaagacaag tttatacagg
     4201 ttcacctttg agatcttttg ttgaaatgga taaaagataa taaaaaccaa aataatattt
     4261 aaaaccactt caatcggagt ggtttttttt tgttagttgt ttaggcgata aatccactaa
     4321 acaaacagtt gcttctatat acttaggttt tgttgctaca ggatttttga gataaattga
     4381 aaatagattt caataaagct tctcttaata gaggagcttt tttatctttg atgctactaa
     4441 aaaatcaact atgttgaaat taagcataaa tgacgaaact tcaagactaa gaactgtcgt
     4501 tcttggtgta gccaatagta atggaccaac accatcaatt gaagaggcgt atgaccctaa
     4561 gtcgttagag catattctgg ctggaactta tccaaaagaa gaagatatgg tggttgaaat
     4621 ggaagctttt aatcagattt ttgagaaata tgaagttacc gttttccgcc ctgaaataat
     4681 agagaactac aatcaaatat ttactagaga tattggtttt gtaattgggg atgtttttat
     4741 taaatcgaat atattgccag aacgagttcg ggaattggat gctattcaat atgtgattga
     4801 tcaaatacaa cccgagaaag tggtaactcc acccgaagaa gttcatatcg aaggtggaga
     4861 tgttatgttg tgggatgatt atgtttttat aggtacctac aaagggagtg attataaaga
     4921 ttatattact gcaagaacca atagagaagg cgtgcagttt ataaaagagt tatttcctaa
     4981 caaaaaagtc aaagaatttg atttgattaa gtcaaaaatt gaagctcgag ataatgcttt
     5041 gcatttggat tgctgttttc aacctgttgg aaaagataaa gcaattattt ataaacgtgg
     5101 gttcagagaa gaagccgatt atctgttcct agttcaactc tttggcgaag aaaatttatt
     5161 tcatattacc cgaaaagaaa tgtattcgat gtgttcgaat gttttttcta ttgatactaa
     5221 tgttgtggtt tctgaaaaga agttttccag actcaacaaa tggttgaaaa aacagggttt
     5281 tattgtagaa aaaatccctt atgccgaaat ttccaaacag gaaggattat tgagatgttc
     5341 tactttgcct ttgatgagag actaaaaaaa gggtttaaaa gaagtttaag gtttaaaaag
     5401 tttaaaagta tatgcttgaa accctaaact tttaaagtaa ctattcaata cctagtaaat
     5461 ttctaacgta ctaagagcat agcggacaat cggtaaaaaa aaaacttgtc ttttagctca
     5521 aagagttttg attgaccaaa agctctaaaa tataaaaatg gaaaaaaccc taaaacagaa
     5581 tatgttttgt acatatatgt acaaaatata tccccttagc cccgtaaact tttaaactat
     5641 taacctcttt taaatccaaa acatgaacca aactacaaat tcaattctaa tgattcgccc
     5701 agtagcgttt cgaatgaacg aacaaacggc tgtaaataat tattatcaaa aagttttgga
     5761 tggattatcc aaagaaacag taaatgctaa agcgcaagaa gagtttgatg gcttggtgca
     5821 aaaactaaaa atggtaggag tcgatgttat cgtagttgaa gatacattga atccagatac
     5881 tcctgatagt atttttccaa acaactggat ttcatttcac gaaagtggcg atgtggtttt
     5941 gtatcctatg tttgctaaaa acaggagaga agagcgtcgg gacgacatac tggatattgt
     6001 agaggaccaa ggttttgtca tcaacgaaat tatggattac acttcggccg aagaagatgg
     6061 tttttatctt gaaggtacag ggagtgtggt tttagacaga gaaaatggaa aggcctattg
     6121 tgctttatcg cctcgggctg acgaagagtt gtttattgaa ttctgtgaag attttgaata
     6181 ttctcctgtg attttcgaag cttttcaaac cgtagatggc gagcgaaaat tgatttatca
     6241 taccaatgtg atgatgtgca ttggcgatac ttttgcagtg atttgcgccg actgtatcga
     6301 tgataaaaaa gaacgcaaaa tggttttaga cagtttgaga ggcgatgata aagaagttat
     6361 tctgattaca gaagctcaag tcaattcctt tgcgggtaat atgctagaag taaaaggcgc
     6421 cgatgataga cgctatttag taatgagtag ctcagcatac caaagtttga caaaaaaaca
     6481 aatcgctcaa atcgaggagc acgttactat cttgagttct aatttggata ctatcgaagc
     6541 ttgtggaggt ggaagcgcac gctgtatgat ggctgagatt ttcttgccga gagggtaaaa
     6601 taatcaatcg tttttcgatg ttcgtttacc gtttattgaa aaacgaacat cgaaaaacga
     6661 ttaatctaaa gtaaatttcc ttttatgatt gttagtagtg cgctaacaat atactgaatt
     6721 cctatagcaa taactataaa tccaacgatt ctagaaatag ctacaattcc agatgctcca
     6781 agaattttag aaagataatg ggcacttctt agaatcacaa aaatagcgat ggcaatggca
     6841 aaaatggcca aacatgaaat catgatttcg tttggcttgt ggtgttcttg atagaatgca
     6901 attaacaaag atatagagcc aggtcctgca agcatcggta tggctagagg ggtcaaagca
     6961 atgtcgtttc ttttttgggc ttccgactct gcttttttgt taattcctct ctttttagta
     7021 aactttccag aaagtaatga aaaacctgaa ctaacaatga tgattcctcc cgcaattctt
     7081 agagcttcta tgctaattcc aaaaaaagtc agcacatatt gaccaataaa aaaggaaaca
     7141 ataagaatta taaagacgtt tattgcggtc cataatgaga ttctagagcg ctctttttta
     7201 gaatcggaat gggtgagccc gacaaaaatt ggtactgttc ctatggggtt tagtacagaa
     7261 aatagagcag caaataaata ggtaaaaaga tccatactgt tttgaattgt ggatgtaaaa
     7321 gtagtgtttt ttattattgt gagattttcg atgcttgatg tttcagagca atcgcattta
     7381 aaaattagtt tttcttttta ccacaaatta cgcaaatttt cgcaaatctg agttagccca
     7441 acgctaattt acctattaat caaaacgaaa cagatgtctt aatttgtgaa aatttgcgta
     7501 atttgtggtt gagataatat ttaattgtaa agggaaataa tttaaaagta aaaagtgatt
     7561 gccctattag cttgctgaaa agcctatttt ttgactcttt tttgcctact tttgcagtcc
     7621 tataagtata gaaatgaaaa ataaaaaaac attggttctc ggcgctagcg cgaagccaga
     7681 aaaatattcc tttaaagcca ttactttatt agtgcaaaaa ggacattccg tattagcaat
     7741 tggtcagaat gctggcgaag tagcaggaat aaaaatccag actaaagcta ttcccttaaa
     7801 aaatatagat actattagtt tgtatctcaa cccaattcgt caacgtgatt attacaatta
     7861 tatagttgaa tctaaaccca agcgtgttat ttttaatcct gggaccgaga atccagagtt
     7921 ctatcaatta ttggaactga atggtattaa ggcagaagtg gcttgtactt tggtattgtt
     7981 gactattaat cagtattgaa ttggttaatt atgttttgct taccatttag tatgtttttt
     8041 acgatgtttt tatcttgcta ggattggatg tttatatgta tttgttaatg aggttgtatt
     8101 tggatttatt ttaaaaaaaa ctttttgtgt gtaaaatgag gaaaaccgta atcgaatgaa
     8161 aatttatgga agtaagtttg tagaatcgac tcaaagtgtg tatttcgaca attatatttg
     8221 cagatagacg gttttttatt ttgaaattat agcgaatata ctttgtaatt tgttgattta
     8281 taacaattaa aaattgatta taagaaagag atagtgtttg ttttttaaaa tgtgagaaat
     8341 ccaagaaaaa aatactatta ggttttgtgt atatttgcct ttaaaataat tttttgagtt
     8401 tgattcactt ttgtgagtca gaaaggcgaa gccatgaaat caaggaaaga aaaagtgtaa
     8461 tcctactcta taaaatctta ggattagttg ttttggtttt tagaaataaa tgtttttcaa
     8521 ttagaattgt attatgaaaa aagacgctaa aatttatatt gctggacatc gaggtatggt
     8581 gggctctgcg atttgtagga cgctttcagc aaaaggatat accaatttga taggggcttc
     8641 aagtgccaac ctagatttgc gaaatcaaga tgccgttcga gattttattt cgaaagaaaa
     8701 accagatgtg gttattgatg ctgctgcaaa agtaggaggg attttggcca acaataatta
     8761 tccttaccag tttattatgg agaatatgca aatccagaac aatttgattg attcggcttt
     8821 gcaatcgggg attgaaaaat ttattttctt agggagttct tgtatttacc ctcgactggc
     8881 tccacagcca ttgaaggaag actatttgct aactagcgat ttagaaccca ctaacgaatg
     8941 gtatgctatt gctaaaatta cgggtgtaaa agcctgtcaa gctattcgca agcaatttgg
     9001 aaaggattac gttagtttaa tgcctaccaa tttgtatggc acctatgata attttgattt
     9061 gaatacttct catgtacttc ctgcaatgat gcggaaattt cacgaagcca aagagaataa
     9121 tcatgctcct gtaacactat ggggaagcgg aactcctttg cgcgaatttt tatttgtaga
     9181 tgatatggct caagcagtcg tttttgcgtt agaaaatact ttgccagatt atttatataa
     9241 tataggaaca ggagttgatt tgaccatcaa agaattagca gaaaccattc agaaaattac
     9301 tgggcatcaa ggtgaaatca tctgggattc cagcaagcct gatggaacgc cacgaaaatt
     9361 aatggatatt tctaaaatgc atgatttggg ttggaaacat aaggtcgatt tagaagaggg
     9421 aattcaattg acttatgatt ggtttttgga gcatgtaaat gattttaaac aagtgaaaat
     9481 gtaaaactat aatctttttt cgataatcgt taaccgcttt tctaacttca aacaacgaat
     9541 taagaattaa gaattacgaa catcgaacaa caaacatcga aaaacaaaca tcgaataacg
     9601 aaaaacgaac aacgacaatg gatacaaaac aaaaagtagc actaataaca ggaattacag
     9661 gacaagatgg atcttatctt gcagaattat tattagaaaa aggatatcat gttcacggag
     9721 taaaaagaag agcttcttct tttaacaccc aacggattga tcatatctat caggatcaac
     9781 atgagaatca tgtgaatttt aaattgcatt atggcgattt gaccgattcg actaatatta
     9841 ttcgaatcat tcaggaagta caacccgatg aaatctataa ccttggagca atgtcccatg
     9901 taaaagtttc gtttgattct cctgaatatg tagctaatgt agatggaatt gggacattaa
     9961 gaatattaga agcagtacga atattgggtc ttgaaaagaa aactagaatc tatcaagcct
    10021 ctacttcaga attgtatggc ggtttggctg aaaataaaaa tgcagcagga ttttatgatg
    10081 aaaactctcc tttttaccca cgttctcctt atggtgtagc taaaatttat ggtttttgga
    10141 ttacgaaaaa ctaccgcgaa gcttatgata tctatgcttg taacggaatc ttattcaacc
    10201 acgaatcgcc aagaagaggg gagacttttg taactcgtaa gattactatg gctacagcag
    10261 ctattgcctt aggcaaacaa gattgtttgt atttaggaaa cctgaactca caaagagact
    10321 ggggacacgc caaagattat gtagaggcga tgtggagaat cttgcaacaa gatgtagccg
    10381 aagactatgt aattgccact ggagttacta cttatatccg tgattttgtg cgtttttctt
    10441 ttgctgaagt gggtatcgaa ttagcctttg aaggagaaaa tgaaaatgaa actgccaaaa
    10501 ttgtagcttg taaccatcct gaatatcaat tgccaatagg tacagtcgtg gtgcgagtag
    10561 atccacaata ctatcgccct acagaagttg atttgttgat tggtgatcca agcaaatcta
    10621 aaaagttggg ttggaaacca caatatgacc ttgctggatt ggttaaggaa atggtggcaa
    10681 gtgatctaga gattgcaaag agagtttaac tgtttataga aaatttggtt ggtcaatcca
    10741 aataaaaaat attttcgatt gtaatttaat tatttcagtt taggaatttt taatgaaaaa
    10801 aataggggta tttattagta gtgttcaatc agaatttgct gaagagcggc gtattctgtg
    10861 tgattacatg acttctgacg cacttctagg gaggtttttt gaacctttta ttttcgagaa
    10921 tgtacctgca gtaaataact cagccatttc agtttatagt aaggaagtag aacaatgtga
    10981 tatttatttg ggtatttttg gcaaacatta cggttttgag gattctaaag gagtttcacc
    11041 caccgaaaga gaatttgatc ttgcaggact ttattcaaaa acaaggttgg tttttttgag
    11101 ttccgatgct gatacggata gagattctaa agaaaggatt ttgattgaaa aagcccagca
    11161 attggtggtt agaaaaagtt ttgcttctcc tctcgaatta aaaacagccg tctatatttc
    11221 attgattcgg tatcttgaag aaaaagaata tattaggact actccttttg atgctacact
    11281 ccattctcgt gctgttattg ccgatttaga tagtcaaaaa attgcagcat ttgttggcgt
    11341 tgcccatacc aaaagaggat ttcctttttc gtctggttct tccaccgaaa cgattcttac
    11401 tcatcttaat ttaatagtac aacaacgaat tacgaatgcc gctattttat tgtttgccaa
    11461 aaatccgcag caatttttta ttagttcaga gattaaatgt gcgcattttc acggaacaac
    11521 aataaccaaa ccgattcctt cttatcatgt ttataaagga gacgtattcg aattagtgaa
    11581 tcaagctgtt gattttgtac tgtcaagaat agatgtttct gttggtacgc gtgaagaaag
    11641 tattcaagtt cctatacgtt atgaattgcc ccctgccgca attacggaag ctatcgttaa
    11701 cgctgtagcg catcgcgatt attccagtaa cggtagtgtt caggtaatgc tttttaaaga
    11761 tcggctcgaa atttggaatc ccggtagttt gccttatgga ttaacaacag ccaaactcag
    11821 aaatccccac agttccattc ctgccaatcc attactggca gagccgatgt accttgcagg
    11881 ttatatcgaa aggatgggaa cgggaacagg cgacattatt cggctttgcg aagaagctgg
    11941 cttagaaaaa gctccagagt ttctccaaga agaaattttt aaaaccatac tttggcgcaa
    12001 aaacacaaca gtaatcgaaa atcaaatcgt agataatgtc gtagataatg tcgtagataa
    12061 tgtcgtagat aatgtcgtag aaaaagaaaa acagttgcta gaattaatac tagccaacaa
    12121 aaaaatatcg gctcaagaaa tggctgttca tttgcaacta acgcaaagaa ccatacaacg
    12181 ctatttgaag ggattacaag atgaaaatcg tttggagaga gtaggctctg ctaaaggtgg
    12241 gttttggaaa ataaagtaaa agactatttt ttaggattaa tcatttaaaa cgttgaatca
    12301 gttagaaaac aggtcaccaa tgaatcaatt gaccttaaac aatttacccc aaaaaacaaa
    12361 cataaatatg caagcagctc at
//
LOCUS       DAOSNN010000042        12373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5105_length_12373_cov_5.205309, whole genome shotgun sequence.
ACCESSION   DAOSNN010000042 DAOSNN010000000
VERSION     DAOSNN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5105_length_12373_cov_5.205309"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1079)
                     /locus_tag="PLQ70_03650"
     CDS             complement(99..1079)
                     /locus_tag="PLQ70_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33741.1"
                     /translation="MKRVFSLLVFVLLLNGCDDGNLTLETIDFEDATTQTCSVNNIIY
                     KLKEKEALLLEIPKTTFVNEPSNIDSATGDYIPTVIDIDNSTYKVVYRFYDGTVAADN
                     ICSTIPPAKPYVSDQWTAISGKIEITTTTITSAGSIPGSTVITGYNHRIIFKNITFDK
                     GNGTQVYETFVFGDYTTSATPLPFGFDKTVEKCTTTKDLYNYISGESLTLENIDDALI
                     VNTETPLNTPRTALIGTVKNKLTYRLFNGLLTPSYFCNTITPVIPTVSEEWNAVSGVA
                     NVSGIIEVTTIKSGTTAFKHTIVIKNATLKKGNSSFKLGDAYIYGELITS"
     gene            1261..2385
                     /locus_tag="PLQ70_03655"
     CDS             1261..2385
                     /locus_tag="PLQ70_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQE33742.1"
                     /translation="MKKIYLDNASTTQLRPEVIQEMTKVLTEDFGNPSSTHSFGRNAK
                     SIVELSRKTIAKSLNVSASEIIFTSCGTEANNWILRSAVKDLNVERIILSKIEHHAVL
                     HTVELLQKEFEIQVDFVSLKSNGEIDLTDLVDLLSQEIKTLVSLMHVNNEIGTLLDVT
                     KVGRICEQHNALFHSDMVQSVGKIEIDLQHSPIDFMVASAHKFHGPKGIGFAFVKKNS
                     GLQPLFYGGEQEKGLRPGTEAVHQIAGMAKALELSYAHLETERNYIFELKKYLISRLE
                     IEFPDFQINGNPDGIYTILNVLLPISETKTAMILFNLDMKGIAVSRGSACQSGSIKPS
                     HVLAEMLSNEDLKKPSLRISFSHFNTKAEIDLLVEALKNI"
     gene            complement(2394..3359)
                     /locus_tag="PLQ70_03660"
     CDS             complement(2394..3359)
                     /locus_tag="PLQ70_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQE33743.1"
                     /translation="MEKSASQKIYLGLFVIFGLLILILAVYFIGDKQKMFGKTNHLEA
                     VFNNVNGLQLGNNVRYSGISVGTVRGIEMINDTTVRVDMIIDKAIFPYIKKDAIATIS
                     SDGLVGNMIINIIPGKENQPSIEPGETIKTFNRIRTEDLLNTLSITNKNAATLTANLL
                     KITDKIIEGKGTIGSLLNDTLISRDLGETMRYLKLTTKSASETVSKIDKIVTSLDNKD
                     NVLGVIKDTAVAHNLKITMANLAQSTSEINKVVTNLNATILNIKDGKGSINYLSNNPD
                     LVRKIDSTMTNLNAASIKLNEDLEAMKHNFLLRGYFKKQEKENKK"
     gene            complement(3367..4119)
                     /locus_tag="PLQ70_03665"
     CDS             complement(3367..4119)
                     /locus_tag="PLQ70_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQE33744.1"
                     /translation="METFQYNTESIIEIKNLKKSYGENHVLNGFNMNLVEGENLVIMG
                     KSGSGKSVMIKCLIGLEDPDSGTICVMGKDISQLKHDELDELRTEVGFLFQGSALYDS
                     MTVRENLEFPLRRHTKKFGVIKDTTPLVMEALENVGLANTINLMPEELSGGMKRRIAL
                     ARTLILQPKIILYDEPTTGLDPITAKEIILLMISIQKKYNTSSIIITHDVDCARAISN
                     RMILLVDGINYVEGTFEEIIALNDPKIQAFFK"
     gene            complement(4123..4905)
                     /locus_tag="PLQ70_03670"
     CDS             complement(4123..4905)
                     /locus_tag="PLQ70_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE33745.1"
                     /translation="METILKIIRIFKSFLLDIGELSLFAGRFFKEVLKRPFEFKEFIR
                     QCYNMGNRSLLLVAVTGFIIGLVFTLQSRPTLQEFGAVSWMPAMVSISIIREIGPIIT
                     ALICAGRIGSGIGAELGSMKVTEQIDAMEVSGTNPFKYLVVTRILATTFMLPILVLFG
                     DAIALYGSYLVENTKGNVSFLLYFNKVAEALEFGDLIPATIKSFFFGFAIGLVGCFKG
                     YNCKKGTAGVGLAANSAVVFTSMLLFIIDFIAVFVTDIFYDL"
     gene            complement(5031..6194)
                     /locus_tag="PLQ70_03675"
     CDS             complement(5031..6194)
                     /locus_tag="PLQ70_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQE33746.1"
                     /translation="MLESNNSIFSAQNITLVHSGEDYFARLEQMILESELEIHIQTYI
                     FEYDATGKKIIAALEKAAAKNVKINILIDGFGSLNFPTEVIHNLSKIGINFRIFSPLF
                     SANSLYIGRRLHHKVIVSDAKKVLIGGINIADKYHGTPKEKPWLDYAVEITDETIAKS
                     LQELCRDMYFKNRIIRRKKIKSFIENSQENCVTILQNDWLKRKNEVFKSYINKISTAE
                     KEIVIVNSYFLPGKKLIKTLKISAQNNVTIKLILSGISDVPMARRASCHLYSKLLRYN
                     IELYEWKKTILHGKTAIIDGNWTTIGSFNLNNLSSFASIEMNVAIESDRFAKEYLVHL
                     SEIINQCERITAETIDTKHNWTSKFTNWASYWLSRIIEIIITFIPHKRFRKFY"
     gene            complement(6322..7074)
                     /gene="tpiA"
                     /locus_tag="PLQ70_03680"
     CDS             complement(6322..7074)
                     /gene="tpiA"
                     /locus_tag="PLQ70_03680"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQE33747.1"
                     /translation="MRKKIVAGNWKMHKNATQTAELLNELKAQVSTKNNAQVIVAPTF
                     VNLALATSLLEGTSIQVSAQNMHQAASGAYTGEIAADMLKSVGVNTVILGHSERRAIF
                     HETDALIASKVDAALQNDMTVIFCFGEELKDRQSKNHFNIVENQLKDGLFHLQAKDWE
                     KIVLAYEPVWAIGTGETASPEQAQEMHEFIRETVRKAYGSDIAEDVSILYGGSVKPDN
                     AKEIFSKPDVDGGLIGGAALNSKDFLAIVNAI"
     gene            complement(7135..7632)
                     /locus_tag="PLQ70_03685"
     CDS             complement(7135..7632)
                     /locus_tag="PLQ70_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQE33748.1"
                     /translation="MNKILLLVFAVISFSCSQAQKNSFSKEALSETLLATDGKQVTFK
                     NILKNHKGKTLVIEIWASWCGDCVKAMPKIKELQTNNPNTAYVFISMDKTADKWKEGI
                     IKHELKGEHYMANDQMKGVFGKAIDLDWIPRYIIIDKTGKIVIYRAIETDFEKINATL
                     KELNK"
     gene            complement(7763..8035)
                     /locus_tag="PLQ70_03690"
     CDS             complement(7763..8035)
                     /locus_tag="PLQ70_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33749.1"
                     /translation="MSSRTDRRKKKWLYIFVSSIIFGVGYYFLNEVSTWFEVPLGVTF
                     HVIFGCTLMAISGIYIGYSIKKMYFTKRRKRSKRIYLEDNKDEKSK"
     gene            complement(8049..9149)
                     /locus_tag="PLQ70_03695"
     CDS             complement(8049..9149)
                     /locus_tag="PLQ70_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQE33750.1"
                     /translation="MKNFIAQFSRLFVGILFIISGLIKLNDPLGFSYKLDEYFSEPVF
                     NMPFFVPYTLAIALFLVILEVVLGVMLLLGYKSKTTIWSLLLLVILFTFLTFYSAYFD
                     VVKDCGCFGDALHLTPWQSFSKDLLLLFFILLLLYNQKYIKSIFSNKTQNILVIASII
                     LCSFMGYWVLNHLPLKDFRPYKVGTNIRKSMEIPDNAPKSVVEMIFIYKVNGVDKEFT
                     EKDLMSLPAGAEFVDRKDKVIVQGYLPPIHDFKLDLNGEDQTNMILAKEKSLLIVAYD
                     LEKANPEGLKKVNELYKNATAKGYYVVLLTGSSADVIENVKKKYNFSFDCLFCDATAL
                     KTIERANPSFVILNYGTVTQKVHYNDITDLNL"
     gene            complement(9178..9726)
                     /locus_tag="PLQ70_03700"
     CDS             complement(9178..9726)
                     /locus_tag="PLQ70_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HQE33751.1"
                     /translation="MNITSQQYDSIVAICRSLFINKMKDYGSAWRILRLPSLTDQIFI
                     KAQRIRSLQENEVRKVDEDETGEFIGIINYSIMALIQLELGVVDQPDLNVEEATELYD
                     AKVKLTKDLMEAKNHDYGEAWREMRVSSLTDLILQKLLRVKQIEDNKGKTLVSEGIDA
                     NYQDMINYAVFALILSNKASND"
     gene            9805..10629
                     /gene="folP"
                     /locus_tag="PLQ70_03705"
     CDS             9805..10629
                     /gene="folP"
                     /locus_tag="PLQ70_03705"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQE33752.1"
                     /translation="MTINCKGQLIDLTSPKVMGILNVTPNSFFDGGKYKDGSQMLSQV
                     AKMLSDGATFIDIGAYSSKPSAEFVSEEEELQRIVSIVNLILEYYPETLISIDTFRSE
                     VARICIENGAAIVNDISAGNLDEKMMETVAKYNVPYIMMHMRGTPQTMQAMTSYSNIV
                     KEMLFYFSEKVSLARSFGINDLIVDPGFGFAKTLDQNYEVLQQLELFKMLELPLLSGV
                     SRKSMIYNLFQQTPEEALNGTTIINTIALNKGAKILRVHDVKEAVECVTIFNKINS"
     gene            10632..11243
                     /locus_tag="PLQ70_03710"
     CDS             10632..11243
                     /locus_tag="PLQ70_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33753.1"
                     /translation="MKYFSLILLFVVFSCGNKEDILLPKSNVTVVSNVVDHSPIYIFF
                     RTKDKDTLAEVNKKNEIISTSWIFNIDKRLPLRLVIPEVMKLQQKKREEVAHKNEKAE
                     NYFSYADSIGKNMAFLPFTKVYYKMGKPKSSFIVFFSKKNKIYVDGFSGSREELKHFL
                     TSFKNKTKMIRFGFDENMSYGTYIENEIFIQSLKIQNKEEFVY"
     gene            complement(11253..11885)
                     /locus_tag="PLQ70_03715"
     CDS             complement(11253..11885)
                     /locus_tag="PLQ70_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33754.1"
                     /translation="MKSKLSLLIILLISNISFSQDKKELFYDYNLTEISKEKFITLKN
                     KDNYALSYENENSIKTYLAEIKTSGKLNFHLLETIKKYLVDLDPKTSFAQTNFIVINY
                     IDNDPSIHTEGYQVPWNVFTKDISKSFKKSEKVTHFYIINPDVKDLYYYHGNKLNWKT
                     DKDHFIKDKFFPFKLNGGFLIIDKNGNYISYKGEYSKKDVLEKLKEMKEK"
     gene            complement(11985..>12373)
                     /gene="rlmH"
                     /locus_tag="PLQ70_03720"
     CDS             complement(11985..>12373)
                     /gene="rlmH"
                     /locus_tag="PLQ70_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQE33755.1"
                     /translation="YIKFDLEIIPDIKNVKNLSESQQKEKEGELILAKITPTDQLILL
                     DENGKTFSSVAFSEELQKKMNSGIKTLVFVIGGPYGFSETVYAKAQGKISLSLMTFSH
                     QMVRLFFIEQLYRGFTILKNEPYHHQ"
BASE COUNT         3947 a         1975 c         2060 g         4391 t
ORIGIN      
        1 aatcaaaagc aaataaaccg aaaagccaca aattaatacc caaaacgatg ggattaattt
       61 gtggcttttc ggtcaaatat aataagatta cttctgaatt aggaggtgat taattcccca
      121 taaatataag catctccaag tttgaagctg ctatttcctt tctttaaagt agcattttta
      181 ataactatag tatgtttaaa agctgttgtt ccacttttta ttgtagtaac ttctataatt
      241 ccgctcacgt tcgccactcc acttacagca ttccattctt cgcttactgt tggaattact
      301 ggtgttattg tgttacaaaa ataacttgga gtaagtaatc cattgaataa acgataggtg
      361 agtttgtttt tgacggttcc aatcaaagca gttcttggtg tgttcaaagg tgtttcggta
      421 ttgacaatta gtgcatcatc aatattttca agcgttaatg attcgccgct tatgtaattg
      481 taaagatctt tagttgtagt acacttttcg actgttttgt caaaaccgaa aggtaaagga
      541 gtggctgagg tggtgtagtc accaaaaaca aatgtctcat aaacttgagt gccattgcct
      601 ttgtcaaaag tgatgttttt aaaaataata cgatgattgt aacctgtaat tacggtgctt
      661 ccaggaatgc ttcctgctga tgttattgta gttgtagtta tttctatttt tcctgatatt
      721 gcagtccatt gatcagatac gtaaggtttt gctggcggaa tggtgctgca aatattatca
      781 gctgctacag ttccgtcata aaaacgatat acaactttgt aggttgaatt gtcaatatcg
      841 ataacagttg gaatgtaatc tccagttgcg gaatcaatat ttgatggttc atttacaaac
      901 gtagtttttg gaatttcaag caataaagct tctttttctt ttagtttgta tataatatta
      961 ttaacgctgc aggtttgtgt agttgcatct tcaaaatcaa tggtttctaa agttaaattc
     1021 ccgtcatcac atccgtttaa taacagaaca aaaaccaata ggctaaaaac tcttttcatt
     1081 ttcaatatat ttctggcaaa aatagtgaaa aaaataccaa ttgatcgtaa tgaaatcata
     1141 attgatgcat catatttggc tatttatttt tcatttggta ttaggatatt gtattttcta
     1201 tcgtgatgtt ttttctgtaa ataaacctgt tgttagtagc tatttcataa ctttgtgcca
     1261 atgaaaaaaa tatatctcga taacgcctcg acgacacaac ttcgtccaga agtcattcaa
     1321 gaaatgacaa aagtattgac agaagatttt ggaaatcctt cgtcaacaca tagttttgga
     1381 cgaaatgcta aaagtattgt agaactttct agaaagacaa tagctaagtc tttgaatgtt
     1441 tctgcttccg aaattatctt tacatcatgc ggaactgaag ctaataattg gattcttcgc
     1501 tcggcagtta aggatttaaa tgttgaaaga ataattttaa gtaaaataga gcatcacgct
     1561 gttttacata ctgttgagtt attgcaaaaa gaatttgaaa ttcaagtaga ttttgtaagt
     1621 cttaaatcta atggtgaaat tgatttgacc gatttagtgg atttgctctc gcaggaaatc
     1681 aaaactttgg taagcttaat gcatgtgaat aacgaaattg gaactctttt ggacgtgaca
     1741 aaagtaggac gaatttgcga acaacataat gccctttttc attcagatat ggtgcaatct
     1801 gttggcaaaa tcgagattga tttgcagcat tcaccaatcg attttatggt agcaagtgct
     1861 cataaatttc acggaccaaa aggaattggt tttgcatttg tgaagaaaaa ttcaggatta
     1921 caacctcttt tttatggtgg cgaacaagaa aaaggcttgc gtcccggaac cgaagccgtt
     1981 catcaaattg caggaatggc aaaagctttg gaactttcgt atgcccattt agaaaccgaa
     2041 agaaattaca tttttgaatt aaaaaaatac ttgatttcca gattagaaat tgaattcccc
     2101 gattttcaaa tcaatggaaa tcccgatgga atttatacca ttttgaatgt tttattaccc
     2161 atttctgaaa ctaaaacagc gatgatatta tttaatttgg atatgaaagg gattgctgtt
     2221 tcgcgtggca gtgcttgcca gtcaggtagt ataaaacctt ctcatgtttt ggctgaaatg
     2281 ctttcgaatg aagatttgaa aaaaccaagt ttacgcattt cttttagtca tttcaatacc
     2341 aaagcagaaa tcgatttatt ggttgaagcg ttgaaaaata tatagttaat cctttatttt
     2401 ttattttcct tttcttgttt tttgaaatag cctcgaagta aaaaattgtg cttcatcgct
     2461 tctaagtctt cgtttagttt gatgcttgca gcattaagat tggtcatagt cgaatcaatt
     2521 tttcgaacta aatcgggatt gttcgaaaga tagttgatgc ttccttttcc gtctttaata
     2581 ttgagaattg ttgcgttgag gttagtaacc actttgttaa tttcggaagt ggattgagct
     2641 aaatttgcca ttgtaatttt taaattatgg gctacagctg tatcttttat aactcccaaa
     2701 acattgtctt tattatctag tgaagttacg attttgtcta tttttgatac cgtttcagaa
     2761 gcgcttttgg tggttagttt caaatagcgc atcgtttcgc ctaagtctct cgagattaga
     2821 gtgtcattca acaaagaacc tattgttcct ttgccttcaa tgattttatc ggtaattttg
     2881 agtaaattgg ctgttagtgt tgctgcattt ttattggtta tactaagtgt gtttaaaagg
     2941 tcttcggttc gaatgcgatt gaaagtctta atggtttcgc cgggttcaat tgaaggttga
     3001 ttttctttac ctggaataat atttataatc atatttccta ctaaaccatc cgagctgatt
     3061 gtggcaatag catctttttt tatataaggg aaaatggctt tgtctataat catatcgacc
     3121 cgaacggtgg tgtcgttgat catttcgatt cctctaacag ttccgacgct aattcctgaa
     3181 tagcgaacat tgtttcctaa ttgtaaaccg tttacgttgt taaaaacggc ttctaagtga
     3241 tttgttttgc caaacatttt ttgcttgtcg ccaataaaat aaacggcaag tattaaaatc
     3301 aataatccga agatgacaaa tagacctagg tatatttttt gtgatgctga tttttccatt
     3361 ttgtatttat ttaaaaaagg cttgaatttt agggtcattt aaagcaatta tctcttcaaa
     3421 agttccttca acataattta tcccatctac taataaaatc attcgattgg aaatggctct
     3481 ggcacaatct acatcgtgag ttataatgat ggaagaagta ttgtattttt tttgaataga
     3541 aatcattagc aaaattattt ctttggcagt aattgggtct aatcccgttg ttggttcgtc
     3601 atacaaaata attttgggtt gcaaaatcaa ggttcttgct aatgcaattc gacgtttcat
     3661 tcctccagaa agctcttctg gcattaaatt gatggtgtta gccaagccaa cattttctaa
     3721 ggcttccatt actaaaggcg tggtgtcttt tatgactcca aactttttgg tatgacgtct
     3781 cagcggaaac tctagatttt ctcgaactgt catcgaatca taaagcgcac ttccttgaaa
     3841 aagaaatcca acttcggttc gaagttcatc gagttcgtcg tgtttcaatt ggctaatgtc
     3901 ttttcccatc acgcaaattg tgccgctatc agggtcttcg agtccaatca aacatttaat
     3961 cattacagat tttcctgaac cagattttcc cataataact agattttcac cttcgactaa
     4021 gttcatattg aagccattta aaacatgatt ttctccataa ctttttttca ggttcttaat
     4081 ctctatgatt gattcagtat tgtattgaaa ggtttccata atttataaat cataaaaaat
     4141 gtcggttaca aaaacagcaa taaaatcgat tataaaaagc aacatcgagg tgaaaactac
     4201 agcagaatta gcggcaagac ctacgccagc agtgcctttt ttacagttat accctttaaa
     4261 acaacctact aatccgatgg caaaaccaaa gaaaaatgac ttgatggttg cgggaattaa
     4321 gtcaccaaat tctaaggctt cggcgacttt attaaaatac aataaaaacg aaacatttcc
     4381 tttggtgttt tctaccagat aggaaccata aagagcgatg gcatcaccaa aaagcaccaa
     4441 aataggaagc ataaaggtag tggccaaaat tcgggtcacg actaaatatt tgaacggatt
     4501 ggttcctgaa acttccattg catcgatttg ttcggtgact ttcatggatc ctaattctgc
     4561 tccaatacca gaaccaattc ttcctgcaca aatcaaagca gtaatgatag ggccaatttc
     4621 tcgaataatc gaaatgctga ccatagctgg catccaagaa acggcgccga attcttgaag
     4681 tgtagggcgt gattgtagcg taaatactaa tcctatgata aatcctgtaa cggctactaa
     4741 aagaagagag cggtttccca tattgtagca ttgtcggata aattctttga attcgaaagg
     4801 acgttttaaa acttctttga aaaaacgccc agcaaaaaga gatagttctc caatatcaag
     4861 taggaaggac ttaaaaatgc gtattatttt taaaatagtt tccatttcaa aaagggatta
     4921 tttttaaaaa gcgcaactta tttataagtg tataagatat aaacgaattg gagtaaatat
     4981 acgaataatc tgttgaatat cagtaaattt agtgatgtat tttttattct ttaatagaat
     5041 tttctaaatc ttttatgcgg aatgaatgta attattattt cgattattct ggacagccaa
     5101 tacgaagccc aattagtaaa ttttgaagtc caattatgtt ttgtatcaat ggtttcagcc
     5161 gtaattcttt cgcattgatt gatgatttca gaaagatgta ctaaatattc tttggcaaat
     5221 ctatccgatt cgatggctac attcatttcg atgctggcaa acgaactcaa attgttgaga
     5281 ttgaaagagc caattgttgt ccaattgcca tcaataattg ctgtttttcc gtgcaaaata
     5341 gtttttttcc attcgtataa ttcaatattg tatcttaaaa gtttagaata caaatgacaa
     5401 ctagctcttc tcgccatcgg tacgtccgag attcccgaaa gaattaattt gatggttaca
     5461 ttattttgag ccgatatttt taaggtttta atcagctttt taccaggcaa aaaataacta
     5521 tttacaatta caatctcttt ttcggcagtg ctaattttgt tgatatagct tttgaaaacc
     5581 tcgttttttc gttttagcca atcattttga aggatagtaa cacaattttc ttgggagttt
     5641 tctatgaaag atttgatttt ttttcttcga ataattctat tcttgaaata catatctcta
     5701 cacaattctt ggagagattt tgcaatagtt tcgtctgtaa tttcaaccgc atagtcaagc
     5761 caaggttttt cttttggagt tccgtggtat ttatcggcaa tattaattcc gccaattaaa
     5821 actttttttg catcagaaac gattacttta tgatgcaatc ttcgtccgat atacaatgaa
     5881 ttggccgaaa ataaagggga gaagattcta aaattgattc ctattttaga taagttatga
     5941 atgacttctg ttgggaaatt taatgaacca aaaccatcaa tgagaatgtt gatttttaca
     6001 ttttttgcag cggctttttc taaagcagca attatttttt ttcctgtagc atcatattca
     6061 aaaatataag tttggatgtg aatttctaat tctgattcta aaatcatctg ttcaagtctg
     6121 gcaaaatagt cttcgccact atgaacaagt gtgatatttt gagcagaaaa aatggagtta
     6181 ttggattcta gcattacaat ttttgaaaat gatgcaactc aaatatacga aaatagtgct
     6241 aaaatcagtt gaatataaaa ttaaaaaatc cccaactatt ttcataattg aggattccca
     6301 tataaagttt aatctaaatt cttagatagc gtttacaatg gccaaaaagt cttttgaatt
     6361 aagtgctgct ccaccaatta agccgccatc aacatctggt ttagagaaaa tttctttggc
     6421 attgtcaggt ttaacacttc caccataaag gatagaaaca tcttcggcaa tatcacttcc
     6481 ataagctttg cgaaccgttt ctctgatgaa ttcgtgcatt tcttgtgctt gttctggact
     6541 agctgtttca cctgttccga ttgcccaaac gggttcgtaa gccaatacaa ttttttccca
     6601 atctttagct tgaagatgaa acaaaccatc tttcaattgg ttttcaacaa tattgaaatg
     6661 attttttgat tgacggtctt tcaattcttc gccaaagcaa aaaataacgg tcatatcatt
     6721 ttgtaaagca gcgtccactt tgcttgcgat caaagcatca gtttcgtgaa aaatagctct
     6781 acgctcagaa tgtccaagga ttacagtgtt tacaccaaca ctttttagca tatcggctgc
     6841 aatttctcct gtatatgctc cactagctgc ttggtgcatg ttttgagccg aaacttgaat
     6901 tgaggttcct tctaataagc ttgttgctaa agctaaatta acaaaagtag gtgctacaat
     6961 tacttgtgca ttatttttag ttgaaacttg agcttttaat tcatttaata attctgctgt
     7021 ttgagtagca tttttatgca ttttccagtt tcctgctaca atcttctttc tcattttaat
     7081 tgtgttttat tgtttattgt ttgtttttta ttgttttgaa attgacattg ttttttattt
     7141 attcaactct ttcaaagtgg cattaatttt ttcgaaatcg gtttcaattg ctctgtaaat
     7201 tacaatttta ccagttttgt caatgatgat atatcttgga atccaatcta agtcgatggc
     7261 ttttccgaac acgcctttca tttggtcatt agccatataa tgctcgcctt tgagttcgtg
     7321 ctttataatt ccttctttcc atttatcagc tgttttatcc attgaaataa aaacataagc
     7381 tgtgtttgga ttattggttt gtagttcttt tatttttggc attgctttga cacaatcgcc
     7441 acaccaagat gcccaaattt cgatgacaag cgttttgcct ttgtggtttt ttaaaatatt
     7501 tttaaaagtg acttgttttc cgtcggttgc tagtaaagtt tcagataaag cttctttcga
     7561 aaaggagttt ttttgggctt gagaacaaga aaaactaatt acagcaaaaa ctaatagaag
     7621 tattttattc atttttaaaa attttgccca aagttacatt ttaaaaatag aataacgaag
     7681 ggtaaagtgc attatttcag ggatttgtac tatttcgatt tcgtaatttg ttagtgtatt
     7741 caattacaga tgagtattgt ttttatttcg atttttcatc tttattatcc tctaaataga
     7801 ttcgtttgct tctttttctc cttttagtaa aatacatttt tttaatggaa tagccaatat
     7861 aaattccaga aattgccatt aaagtacatc caaaaatcac atgaaatgtg actcccaaag
     7921 ggacttcaaa ccaagttgaa acctcgttta gaaaataata tcctactcca aaaataatag
     7981 aactcacaaa aatgtatagc cattttttct ttcttctatc tgttcgacta ctcataatta
     8041 actgtaaatt aaaggttcaa atctgtgatg tcattgtagt gaactttttg agtaacagtt
     8101 ccgtaattca gtataacaaa acttggatta gctctttcga ttgtttttag ggctgtggca
     8161 tcacagaaga gacaatcaaa actaaagttg tatttctttt ttacattttc aataacatca
     8221 gccgaagagc ctgttagtaa tacaacataa tatccttttg cagtagcatt tttatacaat
     8281 tcgtttactt ttttcaaacc ttctggattt gctttttcta agtcataagc cacaatcagg
     8341 agtgattttt ctttagctaa aatcatattg gtttgatctt cgccgtttaa atctaattta
     8401 aaatcgtgaa taggaggtaa gtagccttga acgataactt tgtcttttct atccacaaat
     8461 tcagctcctg ccggaagaga catcaaatct ttttcggtaa attctttgtc aacgccattc
     8521 actttataaa taaaaatcat ttcaaccaca ctttttggag cattgtccgg aatttccata
     8581 gattttcgga tgttagttcc tactttatag ggtctaaaat cttttaatgg caaatgattt
     8641 aaaacccaat atcccataaa tgagcacaaa ataatgcttg ctataaccaa aatattttga
     8701 gttttattcg aaaaaattga tttaatatat ttttgattgt acagcaatag tagaataaaa
     8761 aacaataata gcaggtcttt tgaaaaagat tgccaaggcg ttaaatgtaa agcatcgcca
     8821 aaacaaccac agtctttaac tacatcaaaa taggccgaat aaaaagtcaa aaacgtgaat
     8881 aaaataacca ataaaagcaa actccaaatg gtggtttttg atttataacc caaaagaagc
     8941 attactccca aaaccacttc taaaatcact aagaacaaag caattgccaa tgtgtaaggt
     9001 acgaaaaaag gcatgttaaa tacaggttcg ctaaaatatt cgtccagctt ataggagaat
     9061 cctagtgggt cattgagttt gattaagccc gaaataatga ataaaatccc aacaaaaagt
     9121 cttgaaaatt gcgcaatgaa atttttcatt ttttatataa tttgttacaa aaattggtta
     9181 gtcatttgaa gctttattgc ttaaaatcaa agcaaaaaca gcataattaa tcatgtcttg
     9241 ataattagca tcaatccctt ccgaaactaa tgtttttcct ttattgtctt caatttgttt
     9301 cactcgaagt agtttttgca aaatcaaatc ggttaaagaa ctcacacgca tttcgcgcca
     9361 agcttcgcca tagtcatgat ttttggcttc catcaaatct ttggtcaatt ttactttggc
     9421 atcatacaat tcggttgcct cttctacatt caaatcgggt tgatctacaa cgcccaattc
     9481 gagttgaatc aatgccataa tcgaataatt aatgattccg atgaattctc cagtttcgtc
     9541 ttcatcgact tttcgaactt cgttttcttg taaacttcta attctttggg ctttgataaa
     9601 gatttggtct gttagcgaag gcagtcttaa aatgcgccaa gcactgccat aatctttcat
     9661 tttattgata aatagtgagc gacaaatggc aactatgcta tcgtattgtt gtgaagtaat
     9721 gttcatttaa tgatgtatat ttgtcacaaa aataagtata attattttgt tttaagaatg
     9781 atgacaaacg aaatttgatt taagatgaca ataaactgca aaggacaact tatcgatttg
     9841 acatcgccta aagtaatggg gattttgaac gtgactccaa attccttttt tgatggagga
     9901 aagtataaag acggaagtca aatgctgtcg caagtagcga aaatgttgag cgatggagca
     9961 acatttattg atattggtgc ctattccagt aagccgagcg ccgaatttgt ttcggaagag
    10021 gaagaattgc aaagaatagt ttccatcgtt aatttgattt tggaatatta tcccgaaact
    10081 ttgatttcga ttgacacttt tagaagcgaa gtggcacgaa tttgtatcga aaacggtgcg
    10141 gcaattgtta acgatatttc ggcaggaaat ctggacgaaa agatgatgga aactgttgca
    10201 aaatacaatg ttccatacat aatgatgcat atgcgaggca cgccacaaac gatgcaagcc
    10261 atgacaagtt atagcaatat agttaaggaa atgcttttct atttttctga aaaagtgtct
    10321 cttgctagaa gttttggtat caacgatttg attgttgatc ctggatttgg tttcgccaaa
    10381 actttagatc aaaattatga ggttttgcag caattagagt tgtttaaaat gttagaattg
    10441 cctttgctat cgggtgtttc tagaaaatct atgatttata atttgtttca acaaactccc
    10501 gaagaagctt taaacggaac cacaataatc aatacaattg ctttaaacaa aggggcaaaa
    10561 attctacgag ttcacgatgt gaaagaagca gttgaatgcg tgactatttt taataaaatt
    10621 aattcctaaa aatgaaatat tttagtttaa tactcctttt tgttgtgttt tcttgcggaa
    10681 ataaagaaga tattttattg cccaaatcga atgttactgt cgtttctaat gttgtagatc
    10741 attcgccgat ttatattttt tttagaacca aggacaaaga cactttagcg gaagtcaaca
    10801 agaaaaacga gattatttcg accagttgga ttttcaatat tgacaaacga ttgccgttgc
    10861 gattggtcat tcccgaagtg atgaaattgc agcaaaaaaa gagagaagaa gtggctcaca
    10921 aaaatgaaaa agccgagaat tatttttcct atgccgatag tattggcaag aatatggcgt
    10981 ttctgccttt tacgaaagtg tattataaaa tggggaagcc gaagtctagt tttattgtgt
    11041 ttttttcaaa aaaaaataaa atctatgttg atggtttttc aggaagtcgt gaagaattaa
    11101 aacatttttt aactagtttt aaaaacaaga ccaaaatgat tagattcggt tttgatgaaa
    11161 acatgagtta tggaacttat attgagaatg aaattttcat ccaaagttta aaaattcaaa
    11221 ataaagaaga atttgtttat taaaagaata ttttactttt ccttcatttc tttcaatttc
    11281 tctaaaacat cttttttaga gtattcacct ttgtatgaaa tgtaatttcc gttcttatct
    11341 attattaaaa aaccaccatt taatttgaaa gggaaaaatt tgtcttttat gaaatggtct
    11401 ttatctgttt tccagttaag tttattaccg tggtagtaat acaaatcttt aacatctgga
    11461 tttatgatat aaaaatgagt aactttttct gattttttaa aggattttga aatgtccttt
    11521 gtaaaaacat tccaaggtac ttgataacct tcagtatgga tgcttgggtc attgtcaata
    11581 taattaatca caataaaatt tgtttgagca aaagatgttt tcggatccag atcaacaaga
    11641 tattttttaa tagtttctag taaatgaaaa tttaattttc cagacgtttt aatttctgct
    11701 aaatatgttt ttattgaatt ttcgttttca taagacaaag cataattgtc tttgtttttt
    11761 aaagttataa atttctcttt tgaaatttca gttagattat aatcatagaa gagttctttt
    11821 ttgtcttgac tgaatgaaat atttgagatt agaagaataa ttaatagtga aagttttgat
    11881 ttcatgtttt tacaattcaa ttttaaatac gaatcaaata taagaattta atgcttttgt
    11941 ttttggtttt aaattaattg tatgttaaat attttaaacc aaaatcactg atgatgataa
    12001 ggttcattct tcaaaatggt aaatccacga tacaattgtt cgataaaaaa caaacgcacc
    12061 atttgatgag agaatgtcat tagcgaaagc gatattttgc cttgtgcttt ggcataaacc
    12121 gtttccgaaa aaccataagg ccctccaatg acaaaaacca aagtctttat acctgaattc
    12181 attttctttt gcaattcttc tgaaaaagct acgctcgaaa aagtttttcc gttttcgtct
    12241 aataaaatga gttggtcggt tggcgttatt tttgctaaaa tcaattcgcc ttccttttct
    12301 ttttgctgac tttccgacaa gttttttaca ttcttaatat cgggaattat ttctaaatcg
    12361 aacttaatat aaa
//
LOCUS       DAOSNN010000043        12230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5187_length_12230_cov_5.043532, whole genome shotgun sequence.
ACCESSION   DAOSNN010000043 DAOSNN010000000
VERSION     DAOSNN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12230
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5187_length_12230_cov_5.043532"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..573)
                     /locus_tag="PLQ70_03725"
     CDS             complement(13..573)
                     /locus_tag="PLQ70_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQE33756.1"
                     /translation="MAELNTGDSGGGKDKKVRSKKQNSKVDLTAMVDLAFLLITFFML
                     TTTLSKPQAMELGLPDKEKPNEEDQNIKVDENRTMTIMLGDNDKLVRYVGLLATPVAG
                     GAPKDFAYGKEGIRKELLSRKEAVLKYTGDPKKGMIVIIKPGKKSNYRNLVDILDEMA
                     IVDVPTYAIVNDFSPEEIKLLEGNKK"
     gene            complement(593..1219)
                     /locus_tag="PLQ70_03730"
     CDS             complement(593..1219)
                     /locus_tag="PLQ70_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQE33757.1"
                     /translation="MAGKSSKKAASIDMTAMCDVAFLLLTFFILTATAKIPEALPVDT
                     PSSTVQTKLPESNLATLTIGGKGKVFFDLKGREVRIRTLELMSQKYGVEFTDEEKAKF
                     SLMEGIGVPIGNLKQLIGMKTAERSKAGQPGIPKDSLDNQLTDWVKNARIANNEANNA
                     NGSKEELHIAIKGDAKEEYPSIRKVMDILQDQGINSFSLVTGLRGKNF"
     gene            complement(1290..2108)
                     /locus_tag="PLQ70_03735"
     CDS             complement(1290..2108)
                     /locus_tag="PLQ70_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQE33758.1"
                     /translation="MANVKKEKGSGLGGSISLIIIAACIFAGWIIWNFIMGDGANFEG
                     GVNTGHPLPGNYLAMVYKGGPIVPVLMGLLLMVIVFSFERFAVISKAAGKGNLDKFMA
                     DVQKSIKAGDIDGAIVACDKQQGSVANPIRSALVKYQEVKKEGLDSETAAETLHKEIE
                     ESTSLEMPMLEKHMTILSTLVSLGTLAGLLGTVTGMIKAFGALASSGTPDQAMLANGI
                     SEALINTATGISTSALAIIVYNLFTSKIDTLTYSIDEAGNTIVNTYRHFRGTQK"
     gene            2485..4005
                     /locus_tag="PLQ70_03740"
     CDS             2485..4005
                     /locus_tag="PLQ70_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQE33759.1"
                     /translation="MSKKEEFIQHINDGYLSKGESIILGGAILDGEALADAHIKIPLK
                     TLNRHGLIAGATGTGKTKTIQVLSEQLSSFGIPVLMMDIKGDFSGIAKEGEEKSFITE
                     RHTKINIPYKVSSFPVELMTLSEQSGVRLRSTVSEFGPVLFSRILDLNDVQAGVISVI
                     FKYCDDNKMPLLDLKDIKKVINYITEEGKDEIEESYGKISTSTTGTILRKIIELEQQG
                     ADLFFGELSFEIDDLMRIDENGKGYVNIIRLTDIQDKPKLFSTFMLSLLAEIYNQMPE
                     KGDADQPELVIFIDEAHLIFKEASKTLLEQIETIVKLIRSKGVGIYFITQNPMDVPSG
                     VLAQLGLKIQHALRAFTANDRQAIKQTADNYPSSDYYKTDEILTSLGIGEALVTALSE
                     KGTPTPLAATMLRAPMSRMDILTESEIQEINSKSKLVKKYSELIDRESAYEMLTKKIV
                     AAEQEVTQQQEEKAAEKGDKKSSETTGTVTKSVLKVLTSATFIRGAFSVLSKLLRK"
     gene            4032..4400
                     /locus_tag="PLQ70_03745"
     CDS             4032..4400
                     /locus_tag="PLQ70_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HQE33760.1"
                     /translation="MKGYATIGLLILSNVFMTLAWYGHLKFKELKWFENAGLITIVLI
                     SWGLALFEYFFQVPANRIGYEGNGGPFSLVQLKVIQEVITLVIFVGFSLLFFKNETFR
                     WNHFVGFVFLVLAVYFIFKK"
     gene            4397..4762
                     /locus_tag="PLQ70_03750"
     CDS             4397..4762
                     /locus_tag="PLQ70_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQE33761.1"
                     /translation="MKKYLVQKTPFVVPTTDGKLIEEHHGIPTTNNPAISIAHMIAPP
                     HWKEPFQTPEFEEYTYVIKGKKQFIIEGETVVLEAGQSIKIDKNTRVQYSNPFEEPCE
                     YIAICTPAFDFTKVHREEE"
     gene            complement(4961..7348)
                     /locus_tag="PLQ70_03755"
     CDS             complement(4961..7348)
                     /locus_tag="PLQ70_03755"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQE33762.1"
                     /translation="MKTLKKILLSIIAMFVILVLVSIGFYFYSKPTYEGEQKLKNIKN
                     ETTVYFDDFGVPHIYANSQKDAMTALGYVHAQDRLWQMELLRRIAPGRLSEMFGSKLL
                     KTDRFFAGLGIDENSEKAIANLDKNSEAYQLAMAYLDGINQYMEEEKTPIEFQILGLK
                     KDKFTLRDVYNIFGYMSFSFAMAQKTDPLLTDIRDKLGSEYLKDFGLDGSLGITQIKN
                     FDGKAIEYAKISKSVASLLDNSPVPAFIGSNSWVIAPQKTKNGKVLFANDPHIAFSQP
                     CTWYEAHIVVPDYEMYGYYLAGTPFPLLAHNRNYAFGLTMFENDDLDLFQEEDNPKNK
                     NQYKTANGFQNYTIRKETIKIKDSSDVVLEVRETQHGPIMNDLLDGIKGTKAVAMSWI
                     YTQQKNQILEAVYSLSHSENLNDFRKGVSLIAAPGLNIMYGDAKGNIAWNTSGKLYKL
                     DKSINPNFILNGTNGIDDKKEFLDFSKNPHAINPSWNYIYSANNQPEAVDGHLYPGYY
                     LPRDRAKRISTLLQPKNNWTKEDVGKMLNDNTSSITPSITANLIATLDAKSLSNSEKQ
                     AISILKKWKGSNELKDVAPTIYQKWIYNYLKNTFQDELGETDFEMLLSTHIIKQIIEP
                     QSRNENSIWWDDVTTKNQKETRVEILNKSFHEAITALEKQLGKEVNSWTWNRVHTLEH
                     QHPLGKVAVLRPVFNVGVFKVPGSTEVINNLFFSFSYSGEYQVKGGPSTRRVIDFSDI
                     ENSMSIMPTGQSGNPFSKHYNDQAEMYNSGKFRKMKMNKEEIEKTSTKLVFVPIK"
     gene            complement(7527..8576)
                     /gene="queA"
                     /locus_tag="PLQ70_03760"
     CDS             complement(7527..8576)
                     /gene="queA"
                     /locus_tag="PLQ70_03760"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQE33763.1"
                     /translation="MKLSHFQFNLPKELLAEYPAENRDESRLMVIDRKKGTIEHKMFK
                     DVINYFDEGDVMILNNTKVFPARLYGNKEKTGARIEVFLLRELNAEQRLWDVLVDPAR
                     KIRIGNKLYFGDDDSLVAEVIDNTTSRGRTLRFLYDGSYDEFRNKLTELGETPIPKYI
                     NRDVTPEDADRYQTIYAKEEGAVAAPTAGLHFSKHLLKRLEIKGVKFAEVTLHVGLGT
                     FNPVEVEDLSKHKMDSEELKITQEACDVVNEAKGKRKRICAVGTTSMRAIESSVSSQR
                     TLNPYDGWTNKFIFPPHDFSVATCMITNFHTPKSTLLMMISAFCGHDLMRKAYDEAIK
                     EGYKFYSYGDAMLII"
     gene            complement(8727..9977)
                     /gene="aroA"
                     /locus_tag="PLQ70_03765"
     CDS             complement(8727..9977)
                     /gene="aroA"
                     /locus_tag="PLQ70_03765"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQE33764.1"
                     /translation="MNLLLKTNSPFTIHNSQLNITGSKSETNRLLLLQALFPNITLEN
                     TSNSDDSEVMQKALDNSQFTIDNSQLVDIHHAGTAMRFLTAFFAVNEGREVVLTGSQR
                     MTERPIKVLVEALQQLGAQISYENEEGYPPIRIKGQKIIANKVSIPANVSSQYISALL
                     LVAPKLQNGIELTLVGEITSIPYIKMTLALLNDLDIQTSFEGNIIKVLPKQEVASKVM
                     TVESDWSSASYFFSLAALSEPETAITLSSYKQNSLQGDSALVELYGKMGVESKFENNR
                     LTLTRKSNFAQENLTFDLNNTPDIAQTIVVTCLGLGIGCHLTGLHTLKIKETDRLEAL
                     RIELTKLGANISVTNDSLTLVATKEILHNVKIATYNDHRMAMAFAPLALKVPINIENA
                     EVVSKSYPDFWTDLRNIGFQISEI"
     gene            complement(10043..10369)
                     /locus_tag="PLQ70_03770"
     CDS             complement(10043..10369)
                     /locus_tag="PLQ70_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HQE33765.1"
                     /translation="MNLKNSQLEVDTWIKEHGVRYFNELTNMAQLTEEVGEVARIIAR
                     RYGEQSEKESDKNKDLGEELADVVFVVLCLANQTGIDLQAAFDKKMDLKSVRDKDRHK
                     NNDKLK"
     gene            complement(10420..12144)
                     /locus_tag="PLQ70_03775"
     CDS             complement(10420..12144)
                     /locus_tag="PLQ70_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33766.1"
                     /translation="MPYRIGYEKLNDDRVEFSDCYTSNLENGKVVKTKLASEGNFKTS
                     INKYWKEESITMPNVKVGSIVEFKYVLKSENIVEFPDFNFQKDIFVNYSEYTTQIPGF
                     FVYKAIAKRSSELIFESKLERGSLNYTLNDGTMRSENVNFEQAKNTYSAYNIPALKQE
                     PFVDNIENYRLSVTHELEKTQFYQAPVKNYSTTWEGVAKTIYEDKDFGKELQERDYIK
                     QDLPLILKNIESPDKRLDVIFKFVQDKMNWNNKKGYYVDKGVKQAYIDATGNTAEINF
                     ILISMLNFAGIKASPVLLSTLDNGVPVYPNRTIFNYVIAAVEIDGKQVLLDASNKNTT
                     QNILPLYAINWTGRLINQDGTSQEIKLMPSFPSKKSTNMMFAIDEKGKISGKYTVYRS
                     DYEAFEFREKFLGMNQQNYLEKVENDLKGIQISNYGIENSKDLSKPIIENFTFTADNE
                     CEIIGDKMYINPKLFFTQTKNPFVQEERECPVYFGCPKQEKFNFNIDIPQGYELESLP
                     KPIKIQTRENVCTFSFNCLASGSRIQIVITEDINKGMVSSNFYDELKSFFKQMIDKQN
                     EKIVLKKI"
BASE COUNT         3770 a         2206 c         1952 g         4302 t
ORIGIN      
        1 aggttaataa gattatttct tatttccttc taacaatttt atttcttcgg gactaaaatc
       61 attaacaatt gcataagtag gtacatctac aatcgccatt tcgtctaata tatcaactaa
      121 attacgataa ttcgattttt tacctggttt gataattaca atcattcctt ttttaggatc
      181 tcctgtatat ttcaaaaccg cttcttttct tgataataat tctttacgaa tcccttcttt
      241 tccataagca aaatctttgg gagctccacc agcaacagga gttgctaata aacctacgta
      301 acgaactaat ttatcattat ctcccaacat tatggtcatc gtacgatttt catccacttt
      361 gatattctga tcttcttcgt taggtttttc cttgtctggc aaccccaact ccattgcttg
      421 tggtttagac aaagttgtag taagcataaa gaaggtaatc aacaagaagg ctaaatccac
      481 catagcagtt aaatctactt ttgaattttg ctttttactt cttactttct tatccttacc
      541 gcctccactg tcgcctgtat ttaattcagc cattttagtg tgtatttttt aattaaaaat
      601 tctttcctct taaaccagta actaagctaa aactattaat tccctggtct tgtaaaatat
      661 ccattacttt acgaattgat ggatactctt cttttgcatc tcctttaata gcaatatgca
      721 actcttcttt gctaccattt gcattattgg cttcattatt tgcaatacgg gcatttttta
      781 cccagtctgt caattgatta tccaaagaat ctttgggaat tcccggttga cctgctttac
      841 tacgttcagc agttttcatt ccgataagtt gttttaagtt tcctatcggc acaccaatac
      901 cttccattaa agaaaatttg gctttctcct catcggtaaa ctctacacca tatttttgac
      961 tcatcaattc taaagttctt atacgaactt ctcttccttt caaatcaaaa aacacttttc
     1021 ctttacctcc aatagttaaa gtagctaaat ttgattcagg taattttgtt tgtacagttg
     1081 atgatggcgt atctacaggc aatgcttctg gtattttggc cgtagcggtc aaaataaaaa
     1141 aagtaagcaa aagaaaagcc acatcacaca tagccgtcat atcaatagac gctgcttttt
     1201 tggacgattt accagccatt aattttatat ttaaattagt aaggtattag cgcttatata
     1261 aaacgctaat ccttaaaaat tattattaat tatttttgtg tacctctgaa atgtctgtaa
     1321 gtattcacga ttgtattacc agcctcatca atagagtaag tcaaagtatc aatttttgaa
     1381 gtaaacaagt tgtaaacgat aattgctaat gcagaagtag agatacctgt agcagtattg
     1441 ataagtgctt cagaaatacc atttgctaac atcgcttgat caggagttcc tgaacttgct
     1501 aacgcaccaa acgctttaat catacctgtt accgttccta ataatcctgc aagagttcct
     1561 aaagaaacta atgtagaaag aatagtcata tgtttttcta acattggcat ttcaagagaa
     1621 gtagattctt ctatttcttt gtgcaatgtt tctgcagcag tttcactatc taaaccttct
     1681 tttttaactt cttgatattt cactaaagct gatctaattg gatttgcaac agaaccttgt
     1741 tgtttatcac aagcaacaat agctccgtca atatctcctg ctttgatgct tttttgaaca
     1801 tctgccataa atttatccaa atttcctttt ccagccgctt tagaaataac agcaaaacgc
     1861 tcaaatgaga aaacaattac catcaaaagc aatcccatca aaactggtac aattggacct
     1921 cctttataaa ccattgctaa ataatttcct ggtagtggat gtccagtatt tactccaccc
     1981 tcaaagttag ctccatcacc cataataaaa ttccaaataa tccatccagc aaaaatacat
     2041 gctgcaataa tgatcaatga aatacttcct cctaaacctg aacctttttc ttttttaacg
     2101 tttgccattt tttttaattt taatagtttt aataatttat tttattgtta taataaactt
     2161 tgttgaggag caaatttata tttttagttg atataaaaaa acttttttta ctaattcttt
     2221 aataatttaa cttagacttt aaaaagtgac atttatcatc ttttacatta atttcaacaa
     2281 gtacttataa aatccaaatt ttgtagataa aataaggcat taacgactaa atttaagtgt
     2341 atttttaaca ctttttaaaa aatacttcaa cactttttta atccaaaatc tcaaatgaag
     2401 tttctttttt tttattacaa ttatctatca taaataaaac aaaaaaaata ataccttgca
     2461 attgatttta attcctatcc aaatatgagc aaaaaagaag aatttattca gcatataaat
     2521 gacggttatc tatcaaaagg agaaagtata attctaggtg gtgcaatttt agacggagaa
     2581 gctttggctg atgcgcacat taaaatccca ctaaaaacct taaaccgtca cggattgatt
     2641 gcgggtgcaa ccggaactgg aaaaacaaaa accattcaag tcttgtccga acaattatca
     2701 tcgtttggaa ttccggtttt gatgatggat atcaaaggtg attttagtgg tattgccaaa
     2761 gaaggcgaag aaaaatcatt tatcactgaa cgtcacacca aaatcaacat tccttataaa
     2821 gtatcgagtt ttccggtaga gttaatgact ttgtctgaac aaagcggtgt gcgactacgt
     2881 tctaccgttt ctgaatttgg tcctgttttg ttttctagaa tccttgattt gaatgacgtt
     2941 caagctggag taatttcggt gattttcaaa tattgcgatg acaacaaaat gcctcttttg
     3001 gatttgaaag acattaaaaa agtaatcaat tatattaccg aagaaggaaa agacgaaatc
     3061 gaagaaagct atggcaagat ttcaacttct actacaggaa caattctcag aaaaataata
     3121 gaattagaac aacaaggagc tgatttgttt tttggagagc tatcttttga aattgatgat
     3181 ttaatgcgca tcgacgaaaa tggaaaaggc tatgtaaaca tcatccgctt gacggatatt
     3241 caagacaaac cgaaactttt ttcgaccttt atgttaagtt tattggctga aatttacaac
     3301 caaatgcccg aaaaaggcga tgcagaccaa cccgaattgg ttatttttat tgacgaagcg
     3361 catttgattt tcaaagaagc cagcaaaacc ttgttggaac aaatagaaac tattgtaaaa
     3421 ctgattcgtt ctaaaggtgt tggaatttat tttataaccc aaaacccaat ggatgtacca
     3481 agtggtgttt tggcacaatt gggattaaaa atccaacacg ctttaagggc ttttacggct
     3541 aatgaccgcc aagccatcaa acaaactgct gacaattacc cttcatccga ttattacaaa
     3601 actgacgaaa tattgaccag tcttggcatt ggagaagctt tagtcaccgc tttgagcgaa
     3661 aaaggtacac ctactccact agcagcaact atgctgcgtg cacctatgag cagaatggac
     3721 attttgaccg aaagcgaaat tcaagaaatc aattctaaat cgaaattagt aaaaaaatac
     3781 agtgaactta ttgatcgaga aagcgcatac gaaatgctta ccaaaaaaat cgtagccgcc
     3841 gaacaagaag tgactcaaca acaggaagaa aaagcagccg aaaaaggaga taaaaaatct
     3901 agtgaaacga ctggaacagt aaccaaatcg gttctaaaag tactgacaag tgctactttt
     3961 attcgaggag cttttagtgt tttgagtaaa ttattgagga aataaaatca atttcatatt
     4021 attcgcttta aatgaaagga tacgcaacaa taggcttact catattatca aatgttttta
     4081 tgactttggc gtggtatgga catttgaaat tcaaggaact caaatggttt gaaaacgcag
     4141 gtttgataac cattgttctc attagctggg gattggcctt gttcgaatat ttttttcaag
     4201 ttcctgcaaa ccggataggt tatgaaggca atggcggccc attttcttta gttcaattaa
     4261 aggtaataca ggaggtgata actttagtta tttttgtggg tttctcattg ctttttttca
     4321 aaaacgaaac ctttagatgg aatcattttg tagggtttgt atttttagtt ttggctgttt
     4381 actttatttt caaaaaatga aaaaatatct tgtacaaaaa acgccttttg tagttccaac
     4441 taccgatgga aaacttattg aagaacatca cggaattcca accacaaaca atcctgcaat
     4501 ttctattgca catatgattg caccgccaca ttggaaagaa ccttttcaaa cgcctgaatt
     4561 tgaagaatac acctatgtca taaaaggaaa aaagcaattc attatcgaag gagaaactgt
     4621 cgttcttgaa gctggacaat cgatcaaaat tgataaaaat acccgagttc aatattcaaa
     4681 tccgtttgaa gaaccttgcg agtatattgc catttgcact cccgcttttg atttcacgaa
     4741 agtgcatcgg gaggaagagt aaattcaatt tctttaaacc gcagttacta tcttctataa
     4801 aattgtcatt ccgacgaagg aggaatctag tgttagttgc tcacgtttgt tgagattctt
     4861 ccttcgtcag aatgacaaaa tcatcgacaa aatcccttaa cttaatgaca ttgcactatc
     4921 gctcgattcc ttcttagtcg gaattgacaa atagacgtag ttatttaata ggcacaaaaa
     4981 ccaattttgt cgaagtcttt tcaatttctt ccttattcat tttcattttt ctgaatttcc
     5041 ctgagttgta catttctgct tgatcgttat aatgcttgct gaacggattt ccagattgtc
     5101 ctgttggcat aatgctcata ctgttttcaa tatccgaaaa atctattact cttcgggttg
     5161 atggcccacc ttttacttga tattcaccag aatatgaaaa actaaaaaac agattgttta
     5221 tcacttctgt agaacctgga actttaaata caccaacatt gaaaacgggt ctcaaaacag
     5281 caactttccc caatggatgc tgatgttcta aggtgtgcac tctgttccaa gtccaagaat
     5341 tgacttcttt tcccaattgt ttttcgagtg ctgttatcgc ttcgtgaaat gatttattta
     5401 gaatttctac tctagtttct ttttggtttt ttgtggtaac atcatcccac caaatggagt
     5461 tttcatttct gctttgcggc tcaataattt gtttaataat atgagtgctc aaaagcattt
     5521 caaaatcggt ctcgcccaac tcatcttgaa acgtattttt caaataatta taaatccatt
     5581 tttggtaaat agtaggagca acatccttta attcattcga acctttccat ttttttagaa
     5641 ttgaaatagc ttgtttttca ctgttagaaa gtgatttagc atctaatgtt gcaattaaat
     5701 tagcagtaat acttggcgta atcgaagaag tattatcatt caacattttt ccaacatctt
     5761 cttttgtcca attgtttttg ggttgcaata aagtcgaaat ccttttagct ctatctcgtg
     5821 gcaaataata acccggatac aaatgcccat caaccgcttc gggctgatta ttggcggaat
     5881 aaatataatt ccaagatgga tttatagcat gtgggttttt ggagaaatcc agaaattctt
     5941 ttttatcatc aatgccattt gtaccattta aaataaaatt tggattgata gatttgtcta
     6001 atttatagag ctttcccgaa gtattccaag caatatttcc tttggcatca ccatacataa
     6061 tatttaatcc tggcgcagca attagtgaaa ctcctttcct aaaatcattc aaattttcgg
     6121 aatgcgaaag cgaataaact gcttcgagaa tttggttttt ttgttgggta taaatccacg
     6181 acattgctac tgcttttgtt cctttgattc catccaataa atcgttcatt attgggccgt
     6241 gttgggtttc tcgaacttct aaaaccacat ccgagctgtc tttgattttg atggtttctt
     6301 tacgaattgt ataattctgg aaaccgttgg cagttttgta ttggttttta ttctttggat
     6361 tgtcttcttc ctgaaacaaa tcgagatcat cattttcgaa catcgttagt ccaaaagcat
     6421 aattccgatt gtgtgccaaa agtggaaaag gtgttcctgc caaataatag ccatacattt
     6481 cgtaatcggg taccacaata tgtgcttcgt accaagtgca aggttgcgaa aaagcaatat
     6541 gcggatcatt ggcaaacagc actttcccgt tcttggtttt ttggggagca ataacccaac
     6601 tgttacttcc tatgaaagct ggaacaggcg aattatcgag caaactggca actgatttgg
     6661 aaattttggc atactcgatg gcttttccat cgaaattttt gatttgagta attcccagcg
     6721 aaccgtctaa accaaaatct tttagatatt ccgagcctaa tttatctcga atatcagtca
     6781 ataacggatc tgttttttgt gccattgcaa aactaaaaga catatatcca aagatgttgt
     6841 aaacgtctct cagggtaaat ttatcttttt tcaatcctaa tatttgaaat tcgataggtg
     6901 ttttttcttc ttccatatat tgattgattc catcaagata agccatagcc aattgataag
     6961 cctcggagtt tttatccaag ttggcgattg ccttttcgga attttcatcg atgcctaatc
     7021 cagcgaaaaa tcgatcggtt ttcaataatt tcgaaccaaa catttctgat aatcgtccag
     7081 gagcaatgcg tcgcaataat tccatttgcc acaaacgatc ttgagcgtgt acataaccca
     7141 aagcggtcat tgcgtctttc tgggaattgg cataaatgtg tggaacgcca aaatcgtcaa
     7201 aataaaccgt tgtttcattc ttaatattct ttaatttttg ttctccttca taagttggtt
     7261 ttgagtaaaa ataaaaccca attgatacca ataccaaaat aacgaacatg gcaattatac
     7321 taagaagaat cttttttagc gttttcattt aaattaggaa tatcttataa ttattaggac
     7381 tggaaattta acgatttttt agttcatcgc ctacattcct aaaattattt attttacgaa
     7441 gatttattta aaagtactgt aaaagttcaa aaaaaaaatc tcgccattgc tgacgagatt
     7501 tatggagaat ctgaaataaa ttcagattaa ataattaaca tcgcatcacc ataagaatag
     7561 aatttgtatc cttctttgat ggcttcgtcg taggctttac gcattaaatc gtgtccacag
     7621 aaagcagaaa tcatcatcaa taaagttgat tttggggtat ggaaattggt aatcatacag
     7681 gttgccacgc taaaatcgtg tggaggaaaa ataaatttat tagtccaacc gtcataaggg
     7741 tttaaagttc tttgtgaaga aaccgagctt tctatagcac gcattgaggt agttcctacg
     7801 gcacaaatac gttttctttt cccttttgct tcattcacaa catcacaggc ttcttgagta
     7861 atttttaact cctcagaatc cattttgtgt ttagacaaat cttctacctc gactgggttg
     7921 aaagttccta aacctacatg aagtgtcact tcagcaaact tcactccttt tatttctaat
     7981 cttttcaaca aatgcttaga aaagtgcaaa ccagcagttg gtgctgcaac agctccttct
     8041 tctttggcat aaatggtttg gtaacgatct gcatcttctg gagttacatc tctgttaata
     8101 tattttggaa ttggagtttc tcccaattca gttaatttat ttctgaattc atcataagaa
     8161 ccgtcgtata ggaaacgtaa agttcttcca cgagaagtgg tattatctat tacttcggca
     8221 actaatgaat cgtcatcgcc aaaatacaat ttgtttccaa ttctgatttt acgtgctgga
     8281 tctactagta catcccaaag cctttgttct gcatttagtt ctctcaacaa aaaaacttcg
     8341 attcttgcac cagttttttc tttatttcca tacaaacgag caggaaaaac tttagtatta
     8401 ttcaaaatca taacgtcacc ttcatcaaaa taattgataa cgtctttgaa cattttgtgt
     8461 tctatagttc cttttttgcg atcaattacc atcaaacgag attcgtctct gttttcggct
     8521 ggatattctg caagaagttc ttttggtaaa ttgaattgaa aatgtgataa tttcatttga
     8581 aaaagttatg agttatgaat tatgaattat gagttttgag ttcataatct taaatcgtgt
     8641 gcaaatatac gattgcaaaa taggcgttgt caagtgtttc gaggtttatt tttattttca
     8701 ccgcaaagac gctaggtcgc aaagttttat atttcagaaa tttgaaaacc aatgtttctc
     8761 aaatcggtcc aaaaatcagg ataggatttt gaaactactt cggcattttc gatattaatt
     8821 ggcactttta aagccaaagg agcgaatgcc attgccattc tatggtcgtt ataagtagca
     8881 attttcacat tatgaagaat ctcttttgtt gcaactaaag tcaagctatc gttagttact
     8941 gaaatgttgg ctcctagttt tgtcaattca attcgaagtg cttctagcct gtcggtttct
     9001 ttgattttca aggtgtgaag tcccgtcaaa tgacaaccaa ttcctaaacc aagacaagtt
     9061 accacaatag tttgggcaat atcgggcgta ttattcaaat cgaaagtaag attttcctgg
     9121 gcgaaattcg attttctagt caatgtcaat cgattgtttt cgaacttcga ttcaacaccc
     9181 atttttccgt ataattctac taaagcagaa tcaccttgca aactattttg tttgtaactc
     9241 gacaatgtaa ttgctgtttc tggttcggac aaagcagcca aactaaaaaa gtaagatgca
     9301 gaactccaat cggattccac agtcatcact ttcgatgcta cttcttgctt gggaagcact
     9361 ttgatgatat tgccttcaaa actggtttga atatccaagt catttagcaa tgctaatgtc
     9421 attttgatat aaggaatgga ggtgatttcg cccaccaaag tcaattcgat tccgttttgt
     9481 aattttggag caactaacaa taaagccgaa atatactgac tgcttacatt agctggaata
     9541 ctaactttat ttgctataat tttttgtccc ttgattcgaa taggcggata accttcttcg
     9601 ttttcatacg agatttgtgc tcctaattgc tgcaaagctt cgaccaagac ttttattggt
     9661 cgctccgtca ttctttgcga acctgtcaaa accacttctc gaccttcatt tacagcaaaa
     9721 aaagcagtaa gaaaacgcat tgccgttcct gcatgatgaa tatcaactaa ttgtgaattg
     9781 tcaattgtga attgtgaatt atctaatgct ttttgcatca cttcgctatc atcagaattg
     9841 gaagtgtttt ccaaagtaat attcgggaac aaggcttgca ataacaaaag tcggttggtt
     9901 tctgatttgg aacctgttat atttaactgt gaattatgaa ttgtaaatgg tgaatttgtt
     9961 tttaatagta aattcattgt atcgagaata agttatattt agtaagggtg cgaatttaca
    10021 attcataatt cacaattcac aattatttta atttgtcgtt gtttttgtgg cggtctttgt
    10081 ctctaacgga tttcaaatcc atttttttgt cgaaagcagc ttgcaaatcg attccggttt
    10141 ggtttgctag gcataaaacc acgaaaacca catcggctaa ttcttcgcct aggtctttat
    10201 ttttatcgct ttctttttcg gattgttcgc cataacgacg ggcgattatt cgggctactt
    10261 cgcctacttc ttcagtaagt tgtgccatat tggttagttc gttgaagtag cgaacgccgt
    10321 gttctttgat ccaagtgtca acttcgagtt gggagttttt taggttcatt ggttttggtt
    10381 tttgaaaccc caaaggtatt tattctaaaa attgattgct tatattttct tcaaaactat
    10441 tttttcgttt tgtttgtcta tcatttgttt gaaaaagctt tttagttcat cataaaaatt
    10501 agaggaaacc attcctttgt ttatatcttc tgtaatcaca atttgaatcc tactgcccga
    10561 agcgagacaa ttgaaactaa acgtgcacac attttctctt gtttgaattt ttattggttt
    10621 gggcagggat tccaactcat atccttgagg gatatcaata ttgaaattga atttttcttg
    10681 ttttggacaa ccaaaatata caggacattc tctttcctct tgaacaaaag gatttttggt
    10741 ttgtgtgaaa aataattttg ggttgatgta cattttatcg ccaattatct cacattcgtt
    10801 atcggcggta aaagtaaaat tctcaataat tggtttcgat aaatctttgc tattttctat
    10861 tccatagttg gaaatttgaa tcccttttaa atcgttttct actttttcga gataattttg
    10921 ctggttcatt cctaaaaatt tttctctaaa ttcaaaagct tcataatctg acctatagac
    10981 tgtatatttc ccagatattt ttcctttttc atcaattgca aacatcatgt ttgtcgattt
    11041 ttttgatgga aagctaggca ttaatttaat ttcttgagaa gtaccatctt gatttattaa
    11101 tcttccagtc cagtttatgg catataatgg taaaatgttt tgagttgtat ttttattaga
    11161 cgcgtcgagc aaaacttgtt ttccatctat ttcaacggca gcaattacat aattaaaaat
    11221 ggttcggttg ggataaacgg ggacaccatt atccagagta ctcaataaca ctggagaagc
    11281 tttgattcct gcaaaattta gcatagaaat caaaataaag ttaatttcgg ccgtatttcc
    11341 tgttgcatca atataggctt gtttcacacc tttgtccaca taataccctt ttttattatt
    11401 ccagttcatt ttgtcctgca caaatttgaa tataacatcc aatcgcttat ctggtgattc
    11461 tatattttta agaatcaagg gcaaatcttg cttgatataa tctctttctt gtaattcttt
    11521 tccaaaatct ttgtcttcat agattgtttt ggccacacct tcccaagttg tagaataatt
    11581 ctttactggt gcttggtaaa attgagtttt ctctaactca tgagtaacag acaaacgata
    11641 attttcaata ttatctacga aaggttcttg ttttagagca ggaatattgt aagcactata
    11701 ggtgtttttg gcttgttcga aatttacatt ttctgatctc attgtcccgt cgtttaatgt
    11761 ataatttagg gaacctcgct caagttttga ttcaaatatt aattctgatg atcgctttgc
    11821 aattgcttta taaacaaaaa aaccaggtat ttgagttgtg tattctgaat aattcacaaa
    11881 gatgtctttt tggaaattaa aatctggaaa ttcaactatg ttttctgatt tcaaaacgta
    11941 cttaaactct actattgaac ccacttttac attaggcatt gtaatggatt cttctttcca
    12001 atacttattt attgaagttt taaaattgcc ttcgctagcc aattttgttt taacaacctt
    12061 tccgttttcg agatttgaag tataacaatc tgaaaactct actctatcat cattaagctt
    12121 ttcataacct attcgatacg gcactttgta attcgcccat tttaatcctt cctttttagt
    12181 gctggtgcgt taaaatatta ttttaaatta tcaaaaatat tatattgttg
//
LOCUS       DAOSNN010000044        12154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5215_length_12154_cov_4.477395, whole genome shotgun sequence.
ACCESSION   DAOSNN010000044 DAOSNN010000000
VERSION     DAOSNN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5215_length_12154_cov_4.477395"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..1116
                     /locus_tag="PLQ70_03780"
     CDS             553..1116
                     /locus_tag="PLQ70_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQE33767.1"
                     /translation="MKIIKLFFISTILFTISASSQITKGNWMVGGSGSFINYKNTYSD
                     NNTETTQTGFAFELSPNVGYFVVDNFSIGSIVGLSFINPSGSDNNSQSFGLAPFVRYY
                     FRKSDKIINPFLQASYSFNRSKIQLEGGHNKSSEYRLKGGSAFFLNSSVALEITIEYS
                     SSKNNVNNNQYDRFTTGIGLQIHLEKE"
     gene            1138..2442
                     /locus_tag="PLQ70_03785"
     CDS             1138..2442
                     /locus_tag="PLQ70_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33768.1"
                     /translation="MINKIHFIKYVAVLLFSLSLSAQQVSVTPQNVEYTNNGQPKSFV
                     SGCGNIDLASSTSTLISMIVNIDTYTSVINNSESKLYIYTKKSFSDVKTQRYLKKIPS
                     TNWTPGAAPTTLSYAPTVSFSINAADFNATGGTLLLVFESPSGTQYTSPCTFTITKTP
                     PPSFSLSPDSLSLGCGEVTGRIFTITPANIPAGVTPSYSWSFPGWTEVGSTATSKNLQ
                     PSSGLVLPSTITVTQNINGVPTEVKTCTVSRSSIAPTGYISGSATVCTTRTFTFEGLL
                     VGQSVTWNLSNATAGTLSTANGTSTTFTATAGGAVKLIATVTNSCGQSYVKELDLFAG
                     LPPAFSLVRASNEFCDDIKYHYVPYEITNRNPAFTYTYNVTTYPWVKVTQTSQTYNGV
                     TQDVLVFPKTYSGTIDIYVTTSNSCALLPFLLKKTRLVIAVV"
     gene            2385..2717
                     /locus_tag="PLQ70_03790"
     CDS             2385..2717
                     /locus_tag="PLQ70_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE33769.1"
                     /translation="MCTTPFFVEEDPISNCSGLGLRLSSTAEQFTVFPNPADDIVTIE
                     LKDSEKQFEKDVTISGELFDMMGQSKSKIKISDNKATFSVKGLKKGIYVLKILINDQV
                     ENHRIAVE"
     gene            3216..3788
                     /locus_tag="PLQ70_03795"
     CDS             3216..3788
                     /locus_tag="PLQ70_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33770.1"
                     /translation="MKKIKLFFISTILFTLSANSQITKGNWMVGGSGSFSNKEFYNNS
                     FKNEKLKTSELSIKANIGYFIIDKLQAGVRIGYDDYKVRNVGSKDGDRYWVKYGAYTR
                     YYFLKPEKLINIYLDGEYFFGNNAFSNGQYKERLDGYSVSLGPTIFFNSSVAMELGIN
                     YSSAKFRVLNDATENNLQFRVGFQIFLEKN"
     gene            4309..5922
                     /locus_tag="PLQ70_03800"
     CDS             4309..5922
                     /locus_tag="PLQ70_03800"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQE33771.1"
                     /translation="MNQTKYIFVTGGVTSSLGKGIIAASLAKLLQGRGYRTTIQKFDP
                     YLNVDPGTLNPYEHGECYVTDDGAETDLDLGHYERFLNVPTSQANNVTTGRIYLSVIE
                     KERRGEFLGKTVQVVPHITNEIKERMQLLGKSGDFDIVITEIGGTVGDIESLPYIESV
                     RQLVWDLGENNAIVIHLTLIPYLAAAGELKTKPTQHSVKTLMESGIKADILVCRTEHQ
                     ISQEIRNKLALFCNVKKEAVIQSIDASTIYEVPNLMLEEGLDVVALKKLDLPKKAAPD
                     LKNWNTFLRRLKNPKHTINIGLIGKYVEMQDCYKSILEAFIHAGAANETKVNVISIHS
                     EYIDLENIKEKLQDLDGILVAPGFGERGIEGKIEAIHYARENKVPFLGICLGMQMATI
                     EYSRNVLGYADANSTEMNPDTTNPVVNLMEDQKTVTDKGGTMRLGAWKCEIKPDTLAH
                     RIYGKTTISERHRHRYEFNSHYLAELEKAGLKASGVNPETGLVEIVELEDHPFYIGVQ
                     YHPEYKSTVENPHPLFVNFVAAVVKAKKK"
     gene            6100..7998
                     /gene="yidC"
                     /locus_tag="PLQ70_03805"
     CDS             6100..7998
                     /gene="yidC"
                     /locus_tag="PLQ70_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HQE33772.1"
                     /translation="MEEKKLDLNSIIGFVLIFGILIWIMYQNKPSEAQIAAEKAKKEL
                     VATQKAKKAIQAAVAPVAVVAGDSTQLVQLQKTLGGFAYSATLPSAKEEVTTIENEKV
                     ILKIANKGGYIVEATLKNLEKFKKGSGQLVELIKNNNANLNIQLQTNDNRTLNTKDLY
                     FEPTITKVGADQILSMKLKAGANEFLEYKYILKPNDYMVGFDLRSQGLNKVLNTSKPL
                     DLEWSMKTYRNEKSVSYEDRYTDIRYEYEDGKDNSTGLGKDTEDTPVKVSYIAFKQHF
                     FSSILLTDKPFETSKLHSTKLVNDETIDTVFTKQLKANVPLAFSNGELDYKMSWYFGP
                     SDYKTLKQYDKNLEKIIPLGWGIFGWINKFIFIPLFGFLSSTLGLSLGIAIIIFTILI
                     KVAMSPITFKSFLSQAKMKVLRPEITELGEKFKKDPMKKQQETMKLYNKAGVNPMAGC
                     IPALIQLPFMYASFQFFPSAYELRQKGFLWADDLSSFDAVVKLPFHIPLYGDHISLFP
                     VLASIAIFFYMKMTSGDQQMAAPQQEGMPDMAKMMKMMIYISPIMMLFFFNSYGAGLS
                     LYNFVSNLITIGIMLVIKRYFIDSDKIHAQIQENKLKEPKKQSKFQRKLQEVMEQAEA
                     EKAKKAKK"
     gene            complement(8080..8772)
                     /locus_tag="PLQ70_03810"
     CDS             complement(8080..8772)
                     /locus_tag="PLQ70_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQE33773.1"
                     /translation="MKKIIVLLLIGNFGVLNAQNSIEIPPGTIQLNDSLFIDVSPVTN
                     STFTEYLTAKKVLKNKGYDSFSEFTKATNEKGFPIEMKVIFPPLLIDFYSNNNYLTEL
                     GYWSGYQFQYHPVLNVSKEQAINFCKWRTEMVSHLWKYDEKHALNKNQSDKINYRLAT
                     ENELILAKTFFSNSNTIIEFKEKLLDIKLKKNATQFTIFPVKELTTSEKLFNFNSNYG
                     FTGFRCICEIEK"
     gene            complement(9574..10632)
                     /locus_tag="PLQ70_03815"
     CDS             complement(9574..10632)
                     /locus_tag="PLQ70_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HQE33774.1"
                     /translation="MINLFNTHIETLSIHRVGNKSRNEAIFLSEQPFNLNDEIVPLIK
                     EYFFKPFREKEENYFQFAHEVDLEYNDMFKYATQIFENPSSIHEVSKQITTHLFEQSN
                     HPHIKNGEVYVTHLTNLNIDNNVVDAIGIFKSELQSDFLQFEEKESNLEMVLQHGINL
                     SKLDKGCIIFNYKKEEGYKILTVDSNRYDARYWLEHFLSVDAFEDENFITKKYLKFCQ
                     GFAKDVVFPAEDKKEEVMFMNRSVNYFAKNDQFEESNFLNEVLDNPDLIPEFKNYKVD
                     KGEKYSIEDVTTFPIANAAVSDARKSIKNVINLDTQIQIKMDFINPESAEKFVEKGWD
                     EEKQMYYYLVYFNKEVKS"
     gene            complement(10724..11266)
                     /locus_tag="PLQ70_03820"
     CDS             complement(10724..11266)
                     /locus_tag="PLQ70_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006263712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33775.1"
                     /translation="MVYKFRVILDAEEDIFRDIAILAEDTLEDLHNAIFNSFGFDGME
                     VASFYTCDDTWNQEDEIPMFDTGDIPGEQKTMSDYQLSDILDQENTKIIYVYDFINMW
                     TFLVELASVEEITVGAIYPETLFSVGEIPAEALEKHFIAEDDENESYNEFEDDLDEED
                     LDMFSGDDSFEDYGFEENWN"
     gene            complement(11370..11975)
                     /locus_tag="PLQ70_03825"
     CDS             complement(11370..11975)
                     /locus_tag="PLQ70_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HQE33776.1"
                     /translation="MPIEKIKRNYRKTKYILYKETLVDWKEHFWAFLGSFVAIGILAY
                     IQSIHFTGNDAVYLIGSFGASSVLVYGIIQSPFSQPRNLIGGHLISAFIGVSIHKLVP
                     DIIWIAAPLAVSISIVLMQITKTLHPPGGATALIAIIGSDKIKALGYLYLFSPVLIGV
                     LILLIIALIFNNMTSNRSYPSHKQYHDIRKRLIRKIKKNKY"
     gene            complement(11982..>12154)
                     /locus_tag="PLQ70_03830"
     CDS             complement(11982..>12154)
                     /locus_tag="PLQ70_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HQE33777.1"
                     /translation="LSASIHAPFTAIFLVCGLTNNYTLFIPLLAVSLISKYTAKMIYP
                     FTVYTFSPSIAN"
BASE COUNT         4267 a         1834 c         2085 g         3968 t
ORIGIN      
        1 cgaatctcgt tttacgagag ttgtgaaaga aaatgctgaa ttaggagctg cgttattgac
       61 tcaagcccaa gaagaagtag attctaaatg ggaaagatta gaactgtaca gagatttgta
      121 ataggattta ttaatttaaa actgccctaa caaagcagca aagattgtaa attgaaaaac
      181 ctactcggga tttaaaatct tgggtaggtt tttttatgaa ttttaagtgc aaaaatgaaa
      241 aaaagttgtg ccaaatgtgg aaaaccgtaa aatagaatat tgtggacttt gtaattttga
      301 aactataatt aaaaacgtac aaaaaaagaa tgtttttgct tatataaaaa aagaaaaaac
      361 ttatattttg gcaagaaatt tgtaagtaaa tatttagtga tattattgca aatagttaat
      421 taacaaaacg ctaaaaaaca ctaacgttcg ctatatgaaa atacaaaaaa agaggctgtt
      481 gcaagaccac agcccacccc tcaaaaaaca gattaatcat tattaacttt tataaatctt
      541 aaatatataa atatgaaaat aattaaatta ttttttatct ctacaatttt atttacaatt
      601 tctgctagtt cccaaatcac aaaaggaaat tggatggtcg gaggaagtgg aagttttata
      661 aactacaaaa acacatattc agacaataat actgaaacaa ctcaaactgg ttttgccttt
      721 gaattatcgc caaatgtagg gtattttgtg gttgacaatt tttcaatagg aagtattgtc
      781 ggtctttctt ttataaaccc ctctgggtct gataataata gccagagttt cggattagcg
      841 ccttttgtta gatactattt tagaaaatct gataagatta ttaacccttt tcttcaagca
      901 agctatagtt ttaatagaag taagattcaa ttagaaggtg gtcataataa atcgtctgaa
      961 tatagactta aaggaggttc ggcctttttt ttaaacagta gtgttgcact tgaaataaca
     1021 attgaatata gctcttcaaa aaacaatgtt aataataatc aatatgatag gttcacaact
     1081 ggaatcggac tacaaataca tttagaaaaa gaataaaaaa attaaaaaac aaacaaaatg
     1141 ataaacaaaa tacattttat aaaatatgta gcagtattgt tattctcttt gagtctatct
     1201 gctcagcaag tatcagtgac gccacaaaac gtagaataca ctaataatgg acagcctaaa
     1261 tcttttgtga gtggctgtgg aaatatagat ttagcctcaa gtactagcac cttgataagt
     1321 atgatagtca atatcgatac atatacatct gtaattaata atagcgaaag taaattgtat
     1381 atttacacta aaaagtcctt ctctgatgta aaaacacaaa ggtaccttaa aaaaattcct
     1441 tcaacgaatt ggacaccagg agctgcgcct actactttat catacgcccc tactgttagt
     1501 ttttccataa atgccgctga ttttaatgcg acaggcggaa ctttactttt ggtatttgag
     1561 tcaccctcag gcacacaata cacttcacct tgtactttta caataactaa aacacctcca
     1621 ccgagttttt ccctatctcc cgacagtctc agtttaggtt gtggagaagt tacaggaaga
     1681 atatttacaa ttacaccagc caatattcct gctggcgtaa caccatcata cagttggagt
     1741 tttcctggtt ggacggaggt tggatcaact gctacttcca aaaatttgca gccaagttca
     1801 ggtctagtat tgccatcaac aattactgtt actcaaaata ttaatggtgt tcctacagaa
     1861 gtaaagactt gtactgtttc tcgctcatct attgctccta ctggatatat ttctggaagt
     1921 gcaacagtat gtacgactag aacatttact tttgaaggat tattagttgg tcaatcggta
     1981 acttggaatt tgtcaaatgc aacagctgga actttaagta cagcaaatgg tacatcaaca
     2041 acatttacag caacagctgg tggtgcagta aaattaatag ctacagtgac taacagttgt
     2101 ggacaatctt atgttaaaga actagattta tttgcaggtt tgccaccagc ttttagttta
     2161 gttcgggcaa gcaacgaatt ttgcgatgat attaaatacc attatgtgcc atatgaaata
     2221 actaacagaa acccagcatt cacctatacc tataatgtta ctacatatcc ttgggttaaa
     2281 gtgacacaaa ccagccaaac ttataatggt gtaacacaag atgttttagt ttttccaaaa
     2341 acctattcgg gaacaattga tatttatgtt acaacatcaa atagttgtgc actactccct
     2401 tttttgttga agaagacccg attagtaatt gcagtggttt aggtcttcga ttaagcagta
     2461 ctgcagaaca atttacagta tttccaaatc cagcagatga catcgtgaca atcgaattaa
     2521 aagattcaga aaaacaattc gaaaaagacg taacaatttc gggagagctt tttgatatga
     2581 tgggacaatc aaaatcaaaa attaaaattt ctgataacaa ggcgactttt tctgtaaaag
     2641 gattaaaaaa aggtatttat gttttaaaaa ttctcataaa tgatcaagta gaaaaccatc
     2701 gaatagcagt tgaataaaaa attgaattag ttagttgaaa aacctactcg ggatgtataa
     2761 tcttgggtag gtttttttta tggattttga gtgcaaaaat gaaaaaaaat gaaaaaagtt
     2821 gtgccaattg aggaaaaccg taaaatataa tgttgtggac tttgtaattt tgaaactata
     2881 attaaaaacg tacaaaaata taatattttt aatttctata aaaaaaagaa aaaactgatg
     2941 ttttggcaag aaatttgtaa gtaaatattc agtgttatta ttgcaaatag ttaattaaca
     3001 aaacgctaaa aaatactaag attcgctata tgaaaataca aaaaaagagg ctgttgcaag
     3061 accacagccc tcccctcaaa aaacagatta atcattatta acttttataa atcttagata
     3121 tacatatgaa atgagcataa ctgataattg gtcgcaaata tcaatgatga tatgcgaatt
     3181 acatatgacc aataaaaaac aataaatatc aacatatgaa aaaaattaaa ttatttttta
     3241 tctctacaat tttatttact ctttctgcca attctcaaat cacaaaagga aattggatgg
     3301 tggggggtag tggaagtttt tccaacaaag aattttataa taatagcttc aaaaacgaga
     3361 agctaaaaac gtccgaattg tctataaaag cgaatatagg ttattttata attgataaac
     3421 tgcaagcagg tgtaagaatt ggctatgacg attataaagt tagaaatgta ggcagtaagg
     3481 atggagatcg ttattgggta aaatatggtg catatacaag gtattatttt ttgaaacctg
     3541 aaaaactaat taatatttac ctagatggcg aatatttttt tggaaacaat gctttttcaa
     3601 acggtcagta taaagaacgt ctagatggat attctgttag tttaggtcct accattttct
     3661 ttaatagttc tgtcgcaatg gaattgggta tcaattattc atctgctaaa ttcagagttc
     3721 taaatgatgc tacagagaat aatcttcaat ttagagtagg ttttcaaata tttttagaaa
     3781 aaaattaaaa tcaaaaatta aattattatg aacaaattaa aattatttat tgtgttgctt
     3841 cttttggcaa taacaaattc aaatgctcaa gtagtaacat tagattatag ttctctcgca
     3901 tcgtctagtt gtgatgtttt tgagaaaccc gatggcgcgg atatgatatg attgcaaaac
     3961 attctcgtta gcgatagcgc ggatttgcaa tccgtgccca caaacgagag tgaaaaataa
     4021 aaaaataatt tgacgagttt tggaaataat aataagcacg gattgcaaat ccgcgctatc
     4081 gaaattggtt aaacgaaaaa tctactcgag atgtataatc ttgtgtaggt tttttttatt
     4141 tatgattttt gactaaaaaa ctgtaaccaa gtcatacttg tcaataaaat caaaattatc
     4201 tcaatttcaa attagttttt tctcgattta taattgtatt tttgcagcgt ttttgcaaac
     4261 tattcgcttg caattttacc aaacaacaac aactacaaca ccaataaaat gaatcaaaca
     4321 aaatatattt ttgttacagg cggagttacg tcttctttag ggaaggggat tattgcggct
     4381 tcattggcaa aattattaca gggaagagga tatagaacaa caattcaaaa gtttgacccg
     4441 tatctaaacg ttgatcccgg gacattaaac ccttacgaac acggagaatg ttatgtaacc
     4501 gatgatggtg cagaaactga tttggacttg ggacattacg agcgtttctt gaacgttcct
     4561 acatctcagg cgaataatgt taccacggga agaatttacc tttcagttat cgaaaaagaa
     4621 cgaagaggcg aattcctggg gaaaacggtt caagttgtac ctcatattac caacgaaatc
     4681 aaggaaagaa tgcaattgtt ggggaaatcc ggcgattttg acattgtaat tactgaaatc
     4741 ggtggaactg tgggtgatat cgaatcgtta ccatatatcg aatccgttcg tcaattggtt
     4801 tgggatttgg gcgaaaacaa tgccattgta attcacttga ccttaattcc atatttggcc
     4861 gcagccggag aattaaaaac aaaaccaacg caacactctg ttaaaacctt gatggaaagc
     4921 ggtatcaaag ccgatatttt agtttgtaga acagagcatc aaatttcgca agaaatccgc
     4981 aataaattag ccttgttttg caatgtcaaa aaagaagccg taatccaatc tatcgatgct
     5041 tcaaccattt atgaagtgcc taatttgatg ctagaagaag gacttgatgt tgtagcatta
     5101 aaaaaattgg acttacccaa aaaagcagct ccagatttga aaaattggaa tactttctta
     5161 cgcagattga aaaatccaaa acatacgata aatatcggtt tgattggaaa atatgtagaa
     5221 atgcaagatt gctacaagtc aatcttagag gcttttattc acgcaggtgc tgccaatgaa
     5281 accaaagtaa atgtaatctc gatacattct gaatatattg atttagaaaa tattaaagaa
     5341 aaacttcaag atttagacgg aattcttgtt gctccaggtt ttggagaaag aggtattgag
     5401 ggaaaaatag aagctattca ttatgctcgt gaaaataaag tgcccttttt aggaatttgt
     5461 ttgggtatgc aaatggcaac catcgaatat tcaagaaatg ttttgggtta tgccgatgcc
     5521 aattcgaccg aaatgaatcc tgatacgaca aatccagttg tgaacttgat ggaagatcaa
     5581 aaaacagtta ccgacaaagg aggaactatg cgtcttggag cttggaaatg cgaaatcaaa
     5641 ccagacacat tagcacatag aatttacggg aaaacaacta tttctgagcg tcatcgccat
     5701 cgttatgaat tcaatagtca ttatttggcc gaattagaaa aagcgggatt aaaagcttcg
     5761 ggagtcaatc cagaaacagg tttagtagaa attgtagaat tagaagatca ccctttttac
     5821 attggagtac aataccatcc agaatataaa agtacggttg aaaacccaca tccacttttt
     5881 gtgaattttg tagctgcagt ggttaaagca aagaagaaat agtgttcagt gttcagtgaa
     5941 attgtgctaa caaaaaatag aattcagtaa caatttttta taaaattgga ctaacaataa
     6001 ttaatttaaa agtaactaat aaataaacac attcaagact cagtttcctc ctgacattcc
     6061 gtaggaatct agagggctga ggaaaggaaa aaagtagaaa tggaagaaaa aaaattagac
     6121 ctcaattcga ttattggttt cgtattgatt tttggaatct tgatttggat tatgtatcaa
     6181 aacaagcctt ctgaagcaca aatagcagcc gaaaaagcta aaaaagaact tgttgcaaca
     6241 caaaaagcaa aaaaggcaat tcaagcggca gttgcgccag tagcagtagt ggcaggcgat
     6301 tcaactcaat tagttcaatt acaaaaaact ttaggcggtt ttgcttattc tgcaactctt
     6361 ccatcggcaa aagaagaggt taccactatc gaaaacgaaa aagtaattct gaaaattgcc
     6421 aataaaggag gttatattgt tgaagcgact ttaaaaaatt tggagaaatt caaaaaagga
     6481 tcaggacaat tggtagaatt gattaaaaac aataatgcca atttgaatat tcaattgcaa
     6541 accaatgata atcgtacttt aaacaccaaa gacctttatt ttgaacctac aattaccaaa
     6601 gtgggagcag atcaaatcct ttcaatgaaa ttgaaggctg gtgccaatga atttttggaa
     6661 tacaaataca ttttgaaacc aaacgattat atggttggtt ttgatttgcg ttcacaaggg
     6721 ttaaataaag ttttgaatac tagcaaaccg ttagatttag aatggagcat gaaaacctac
     6781 agaaacgaga agagtgtttc ttatgaggat agatataccg acattcgtta tgaatatgaa
     6841 gatggaaaag ataatagtac tggattagga aaagatactg aggatactcc tgtcaaagtg
     6901 agttatatcg ctttcaaaca gcattttttc tcttctatat tattaacaga taagcctttt
     6961 gagacttcaa aactgcattc aactaaattg gtgaatgatg aaacaatcga tactgttttt
     7021 accaaacaat taaaagcaaa tgttcctttg gctttcagca atggcgaact agattataaa
     7081 atgagttggt attttggccc atccgattat aaaacattaa agcagtatga taaaaattta
     7141 gagaaaataa ttcctttggg ttggggaatt ttcgggtgga ttaataaatt tatttttatt
     7201 cccctatttg gatttttgag ctcaacatta ggactttctt tgggaattgc catcattatt
     7261 tttactattt taatcaaggt ggctatgtcg ccaattacct tcaaatcgtt cttgtcacaa
     7321 gccaaaatga aagtattgcg tcctgaaatc actgaattgg gagaaaaatt caaaaaagac
     7381 ccaatgaaaa aacagcagga aactatgaag ttatacaaca aggcgggagt aaacccaatg
     7441 gcaggttgta ttccagcttt gatacagttg ccctttatgt atgcttcgtt tcagtttttt
     7501 ccatcggctt atgaattgag acaaaaaggc tttctttggg cagacgattt gtcttctttt
     7561 gatgcggttg ttaaacttcc gtttcatatt ccgttatacg gagatcatat tagtttgttc
     7621 cccgttttgg cttcgattgc gattttcttt tatatgaaaa tgacttctgg agatcagcaa
     7681 atggctgctc cgcaacaaga aggaatgcca gatatggcca aaatgatgaa aatgatgatt
     7741 tacatctcgc caataatgat gttgttcttc tttaatagtt acggtgctgg tttgagtttg
     7801 tataactttg tttcgaattt gattactatt ggaattatgc ttgttataaa aagatatttt
     7861 attgatagtg ataaaattca cgctcaaatt caagaaaaca aattgaaaga gcctaaaaaa
     7921 caaagtaagt tccaacgaaa actgcaagaa gtaatggaac aagccgaagc tgaaaaggct
     7981 aagaaagcta agaaataatc aaacaaaaaa tcccgagcaa tcgggatttt ttgtttgata
     8041 caaaaaaaga agaatgttgg actgaattat attggcgtat tatttttcta tttcgcaaat
     8101 acatctaaat ccagtaaatc cataatttga attaaagttg aataattttt cggaagttgt
     8161 caactccttt acaggaaaaa tagtaaactg agtggcattt tttttcagtt ttatgtccaa
     8221 gagtttttct ttaaattcaa tgattgtatt tgaatttgaa aaaaatgttt ttgcaagaat
     8281 aagttcattt tcagttgcta acctgtaatt aattttgtca gattgatttt tatttaacgc
     8341 atgtttttca tcgtatttcc ataaatgact taccatttca gttctccatt tacaaaagtt
     8401 aatggcttgt tcttttgaaa cattcaatac tggatgatat tggaattggt atcccgacca
     8461 ataccctaat tctgttaaat agttgttatt tgaataaaag tcaatcaata agggagggaa
     8521 tataactttc atttcaatgg ggaagccttt ttcatttgtt gctttggtaa attcactaaa
     8581 tgaatcgtat cctttatttt tcaaaacttt ttttgcagtc aaatattctg taaacgtcga
     8641 gtttgtaacg ggtgatacgt caatgaataa actatcattt aattgaattg taccaggtgg
     8701 aatttctata ctattttgag cattcaatac cccaaagttg cctattagaa gtaagactat
     8761 tatttttttc atgatttttc taaattgtat atatgtttaa cacattagtc ttatttgtag
     8821 gttaaatata taattaattt tttacaaatt gaaaaataac gagaagtttg tattgatgta
     8881 atttttagga aaaaaggcaa aaaaaaaatc ccgaaaaatt cgggatcata ttgaataatc
     8941 tatttttgaa tatttgaaat caaagtatcg gctattacta ttttgccaag ttttgaatag
     9001 tatatttttt ccttttgttt ccaatgtagt ggttgattaa ggaatcaaat ctgtaaagtt
     9061 gcagatatga tgtaaactat cagctaatca ataatttaaa ataaattaat gaatttttag
     9121 ctcttcatgt ctataacagc tactcgaact ttatactttt tattaggttt tggcattaac
     9181 aacattatgc tttttagtat gaggataagt agtgtcattt ttagcaattt ttgaatgaat
     9241 aataagttta aaaatgtctt ttttgatgta atttaattta tatcttttaa ctctggtggt
     9301 aaaaaaagtt ttatctcttt taaatcagaa gtcggttcta aaccacaaca agaatatttg
     9361 tttaaatata atacagtaaa gctactgtat aagcgattaa aaacaaaata aaatcgataa
     9421 actacatatt tttacataaa aaataattta aaacttacaa aataatatgt attgatgttg
     9481 ttttaacttg ttctagaaca gaacatataa aaaaaatcct caattatttt actaattgag
     9541 gatttaaaca ggtgttattt taagaattta gtactagctt tttacttctt tattgaaata
     9601 aaccaagtag taatacattt gtttttcttc gtcccaacct ttttcgacaa atttttcagc
     9661 actttctgga tttatgaaat ccattttgat ttggatttga gtatccaagt tgattacgtt
     9721 tttaattgat tttctagcat ctgaaacggc tgcattggca ataggaaaag tagtcacgtc
     9781 ttcaatactg tatttttcgc ctttatctac tttgtagttt ttgaattctg gaatcaaatc
     9841 cggattgtcc aaaacttcat ttaagaaatt gctttcttcg aattggtcgt ttttggcaaa
     9901 ataatttacc gaacgattca taaacatcac ttcttctttt ttgtcttcgg caggaaaaac
     9961 tacgtcttta gcaaaacctt gacagaattt caagtatttt ttggtgatga aattttcgtc
    10021 ttcaaaagca tcaaccgaaa ggaaatgttc caaccaataa cgggcatcat aacgattgct
    10081 gtcaacagtc aagattttat atccttcttc ttttttataa ttgaaaatga tacagccttt
    10141 gtccagtttg ctcaggttaa taccgtgttg caaaaccatt tcaaggttag attctttttc
    10201 ttcaaactgc aaaaaatccg attgtaattc gcttttgaaa attccaatag catcaaccac
    10261 attattgtcg atgtttagat tcgtcaaatg ggttacataa acttccccat ttttaatgtg
    10321 tggatgattc gattgttcaa ataaatgagt ggttatttgc ttggatactt cgtgaattga
    10381 actcggattt tcaaaaattt gagtagcata tttaaacata tcattgtatt ccaaatcgac
    10441 ttcgtgtgca aactgaaagt agttttcctc tttttcccga aaaggtttaa agaaatattc
    10501 tttgattaaa ggaacaattt catcgtttaa attaaaaggt tgctcagata agaaaatggc
    10561 ttcgttgcgg cttttatttc ccactcggtg aatggaaagt gtttcgatgt gagtgttgaa
    10621 taaatttatc attgtttttt tgttttttta gacaaaagaa ccaagatgta agacagagta
    10681 gaagtctttc atcttggttc ttgattctta atttcttctc tttttaattc cagttttctt
    10741 caaatccgta gtcttcaaaa ctatcgtctc cgctaaacat atctagatct tcttcgtcaa
    10801 gatcatcttc aaattcattg taactttcat tttcatcatc ctcagcaata aagtgttttt
    10861 ctaaggcttc ggcaggaatt tcaccaacag aaaatagggt ttcaggataa atggctccaa
    10921 cagttatttc ttcaactgaa gccagttcaa caagaaatgt ccacatattg atgaaatcgt
    10981 aaacataaat tattttggta ttttcttgat ccaaaatatc cgaaagttga taatcactca
    11041 tcgttttttg ttcacctgga atatctccgg tgtcaaacat cggaatttcg tcttcttgat
    11101 tccaagtgtc gtcacaagtg tagaaagagg ctacttccat tccgtcaaac ccaaaagaat
    11161 tgaagatggc gttgtgtaaa tcttctaaag tatcttcggc aagtattgca atgtctctaa
    11221 aaatgtcctc ttcggcatcg agaattactc taaatttata aaccataatc tttgttttta
    11281 ttgagaggtc aaaggtaaaa tttaatttta gattttagat ttcagatttc ggatttgttg
    11341 ataagatttc ttttaaccac attctaagct tagtatttgt ttttttttat ttttcggatt
    11401 agtctttttc ggatgtcgtg atattgtttg tggctaggat agcttctgtt tgaagtcata
    11461 ttgttaaaaa ttagagctat aatcagtaaa attaacactc ctatgagtac tggagaaaat
    11521 aggtataaat accctagagc ttttattttg tcagaaccaa taatggcaat aagggctgtt
    11581 gctcctcctg gcggatgcag ggttttggta atttgcatca aaacaataga aattgaaacg
    11641 gccaaaggtg ctgcaatcca aatgatatca ggaaccaatt tgtgtatgct aacgcctatg
    11701 aatgctgaaa ttaaatgtcc tccaatcaag tttcgaggtt gtgaaaatgg actttggata
    11761 atgccataaa ccaaaacact tgaagctcca aaagaaccaa ttaaatagac cgcatcgttt
    11821 cctgtaaaat gaatggattg aatgtaggcc aaaattccga tggcgacaaa cgaacccaaa
    11881 aaagcccaaa aatgttcttt ccagtcgact aaagtttctt tgtaaaggat gtatttggtc
    11941 ttgcggtaat tccgttttat tttttcaatt ggcataagtg gttaatttgc gatacttggt
    12001 gaaaaagtat agactgtaaa aggataaatc attttggccg tatattttga aatcaaggaa
    12061 actgccaaaa ggggaatgaa taaggtataa ttgttggtca aaccgcaaac caagaagatg
    12121 gccgtgaaag gcgcgtggat actggcgctc aaaa
//
LOCUS       DAOSNN010000045        12054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5260_length_12054_cov_4.495458, whole genome shotgun sequence.
ACCESSION   DAOSNN010000045 DAOSNN010000000
VERSION     DAOSNN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12054
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5260_length_12054_cov_4.495458"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLQ70_03835"
     CDS             <1..672
                     /locus_tag="PLQ70_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HQE33778.1"
                     /translation="LFVFFFSNYAFYEHEQYITTGIHQRIGFYLHYISLFITLFFGYK
                     LYQINKENPVFNLFKNKFAVWFAAFLIIYLASTELMLHGLVLSNSPITAQQVQSNEYY
                     KNYNATELPFLKAQIADDAIQSTRTQIIKTGFPILWGILAFAFLIIGIKKRIKAIRII
                     ALSLLGITILKLFLFDISNASETGKIIAFILLGVLILIISFVYQKIKVLVQDDNKPNK
                     TDETE"
     gene            707..1894
                     /locus_tag="PLQ70_03840"
     CDS             707..1894
                     /locus_tag="PLQ70_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33779.1"
                     /translation="MAQHTTTAKIEIPKENGLHKMVLPAEIRSFSKEELGDFRIVDSK
                     EIEVPYYVIPQEYIDKTPHFFSECKIISTNVLPKNKTTLIFENPKTSIDSIVLCITNS
                     DVTKTYSISGSNDQKEWFGLVNNSKLSDLVNTEETVVFKTISFPLTSYRYIKFDFNDK
                     KTLPINILKIGFFASKTTSNILEEIHPKNIKFQQIPAEKKTRIYISFDHPQIVNQIHF
                     KISSPNLFLRNTQIYVNKKKRINQKAVAFPENFSSFELNSNTKNTFTIPQFFGKECFI
                     EIENQDNPPLTFSEIRFFQKPISVIADLKANQKYTVETGNSKLNSPDYDLENFKNRMG
                     SNLPEAKIYDIKHDISQKTNALKKSFWQQSWFMWICIGLGGIVIVFFTMSLVKDMKNN
                     STI"
     gene            1944..2552
                     /gene="udk"
                     /locus_tag="PLQ70_03845"
     CDS             1944..2552
                     /gene="udk"
                     /locus_tag="PLQ70_03845"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HQE33780.1"
                     /translation="MLIIGIAGGTGSGKTTVVHQIMNELPHTEVGIIAQDSYYKASTV
                     ESYEERSKTNFDHPRAIDFDLLVSHLKELKEGKTIHQPVYSFAIHNRTDDTIVTHPRK
                     VMIVEGILILSNPELRDLFDIKIFVHADSDERLIRRLKRDIAERGRDMEEVLNRYQTT
                     LKPMHQQFIENTKAYADIIIPNDKYNTVAIDVVRAVINQRIL"
     gene            2689..3021
                     /locus_tag="PLQ70_03850"
     CDS             2689..3021
                     /locus_tag="PLQ70_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQE33781.1"
                     /translation="MANPYKDKSWFKFLSNKYVWVLLLFSIWMIFLDNYSYFGHRVLD
                     KQIDELEQNKEYYQQEIKKDQENIKKLKNPDQTEKYAREKYYMKKDSEDVYIIEFEGD
                     TIEKDVKK"
     gene            3074..4438
                     /locus_tag="PLQ70_03855"
     CDS             3074..4438
                     /locus_tag="PLQ70_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HQE33782.1"
                     /translation="MKNLFDDFDPVSSKQWKQKIQFELNGADYNQTLIWNSPEDIKVK
                     PFYHSDDYTPTSGINTKATQFLICQNIFVHDLAKSNARAIDSINRGAESIRFTIEDEK
                     IEVAKLLQNLPLDTITIYFNLSFISIPFIKKIEAVAKENKAKIYCNLDPIGQLAKEGN
                     WFETKEKNNFETLNLLSESISNISLLSIDGGLYQNAGANMVQQIAYSLAHANEYFNQI
                     KTINQPIVFQISVGTNYFFEIAKLRALRLIFNLIAKEYNHKFDCHILVSPTKRNKTIY
                     DYNVNMLRTTTECMSAILGGADAIANLPYDALFHKDNEFGNRIARNQLLVLKHESHFD
                     KVNNPADGSYYIESLTTQLAEKALTLFKAIEANGGFIKQLNEGIIKRKIQESADKEQE
                     LFDSGKETLVGTNKYPNKKDRMKDDLELFPFVKIKPRKTLISPIIEKRLSEKMEQERL
                     KEEI"
     gene            4446..4901
                     /locus_tag="PLQ70_03860"
     CDS             4446..4901
                     /locus_tag="PLQ70_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33783.1"
                     /translation="MKNILYTLALLLSLTVFSQQKTIEITNMESGKTVVFKENQRVKI
                     RTLDLKKWVGNLKFSDSLHIIVNNHKLAIDSLQSIKNQPKVLGTVKTVVLISGLAIVG
                     TSLIAASGGSESALLIFMIGSGTTISAGIMEGLNKNYTKRKWMYKVVEK"
     gene            4901..7030
                     /gene="scpA"
                     /locus_tag="PLQ70_03865"
     CDS             4901..7030
                     /gene="scpA"
                     /locus_tag="PLQ70_03865"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HQE33784.1"
                     /translation="MRKNLQHIKLEKSEVRSQKSEVSRKDNFLTAEGIELKPTYSEKD
                     IENLEHLDFGAGFSPNLRGPYATMYVQRPWTIRQYAGFSTAQESNAFYRRNLAAGQKG
                     LSIAFDLPTHRGYDSDHERVVGDVGKAGVAIDSVEDMKLLFDQISLDEMSVSMTMNGA
                     VLPIMAFYIVAAEEQGVNPEQLSGTIQNDILKEFMVRNTYIYPPTPSMKIIADIFEFT
                     SKKMPKFNSISISGYHMQEAGATADIELAYTLADGLEYIRTGLAAGMKIDDFTPRLSF
                     FWAIGMNHFMEIAKMRAGRMIWAKIVKQFNPKDEKSLALRTHCQTSGWSLTMQDPFNN
                     VARTTIEAAAAAFGGTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETKITKTVDP
                     WAGSFYVESLTNEIAQNAWKLIEEIEELGGMTKAIESGIPKLRIEEAAAKKQARIDSN
                     QDLIVGINKYRLKKEDPLQILDVDNQMVRQQQIEQLEKIKSRRNTEKVQLSLKKLTLC
                     AQTGEGNLLEIAIEAARNRATLGEISNALETVFGRYKAQIKSFSGVYSKEIKEDKSFE
                     KAKKMADEFAQKEGRRPRIMIAKMGQDGHDRGAKVVATGYADLGFDVDIGPLFQTPAE
                     AAKQAVENDVHILGVSSLAAGHKTLVPQVIDELKKYGRQDIMVIVGGVIPTQDYQFLF
                     DAGAVAVFGPGTKISESAIKMLEILLI"
     gene            7086..8213
                     /locus_tag="PLQ70_03870"
     CDS             7086..8213
                     /locus_tag="PLQ70_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HQE33785.1"
                     /translation="MSKLPNVTTSIFATMSKMATENNAINLAQGFPNFEVDDKLAQIV
                     AALAQKEVHQYLPIAGYPPLLTKIAKLIFDSYQRKVLPETEILVTTGATQALFTTILA
                     LIKTDDEVIILDPSFDNYEAPTLLCNAKTIRVELNDDYSPNWELIEKAITPKTRMLII
                     NSPHNPTGKIWKENDFQSLENLFEKYPNLLLLSDEVYEFISFEQKHSSIHSRKKIANR
                     SIVVSSFGKSFHITGWRIGYLVAPEYLTKEIKKVHQYLVFCVNSLAQAAISDYLEVAD
                     VTKLGNFYQEKRDHFRALLQNSRFELLPCEGTYFQVVSYASISNENDVNFCKRLITEY
                     GVAAIPISAFYADGKDQKLIRFCFAKDNKTLEEAARRLCKV"
     gene            8286..9167
                     /locus_tag="PLQ70_03875"
     CDS             8286..9167
                     /locus_tag="PLQ70_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE33786.1"
                     /translation="MDFSAKMLRDDALKGKVIVVTGGGSGLGKAMTRYFMELGAKVAI
                     SSRDLEKLQNTAKELETETGGTCLAVQCDVRHYEEVENMLEEVLKTYGKIDVLLNNAA
                     GNFISPTERLSANAFDTVIDIVLKGSKNCTLAFGKHWIDSKQTHATILNIVTTYAFTG
                     SAYVVPSAAAKAGVLAMTRSLAVEWAKYGIRTNAIAPGPFPTKGAWDRLLPGDLAEKF
                     DMAKKVPLKRVGNHQELANLAAYLVSDFSAYINGEVIVIDGGEWLKGAGEFNILEAIP
                     EELWDQLEMMIKAKKRS"
     gene            complement(9205..10494)
                     /locus_tag="PLQ70_03880"
     CDS             complement(9205..10494)
                     /locus_tag="PLQ70_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33787.1"
                     /translation="MKRVIDFVAKYPVLITALVCVVFYFLPFKPKPFGDGEYHEGTIQ
                     LIQYILNGFQGTVRIDKGLFTLFYYLVPYSLGYVFHNDTLFYLLGVAYNSIITCWAIQ
                     YIFASFDLMSFPTRTKFWTIIVLNLFPIHVYYAMGILAESAAFFGVSLFIYAWLKIIA
                     NSNAVKYYLMLSFSLVIVSGTRPNLLPFLVLFTLYFLTIKSEWKNKVIFISSLGLMLL
                     FLALVEKKINNTDGAFKKDIFRRHLLWSRYELRDDPFNWLPQDGQKEFATSDYLHNLA
                     KRDELDSICRVNHFDKTDYYIKWVTNDIIQNPGLTLRQYSLKFFQSQTFVISPIMKYN
                     KSNFVKWSIHIYINAINYLLIFFSIAAIVVMNKQKKYQLMMPFLLFWGWSLVYVFIFH
                     SEQRYMFPVRPIMVFLFGYYLNYYFNGNTPSKNSQVE"
     gene            complement(10503..11504)
                     /locus_tag="PLQ70_03885"
     CDS             complement(10503..11504)
                     /locus_tag="PLQ70_03885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE33788.1"
                     /translation="MKFSLIICTYMRPQSVLQLLQSVQGQTLYPDEILIIDGSTNQET
                     ELALKEHPFQNLHYFAVPPEYRGLTRQRNFGVQKAGSSMEVIAFLDDDTILEADYFKE
                     LIQTFEENPAISGVAGVAINENGWQLAEPNKTYNPNRYYPLDGFVYKEGQRNVVRNYL
                     GLQSNLGPGRMPDYSHGRTCGFPLNGKTYEVDLLIGMSFSFRKKVLDTIRFSSYFEGY
                     GLYEDADFSIRALQFGKNAINTKAQLSHFHHPSGRPNQYKYGKMVVRNGWYVWRAKNP
                     TPSLKAKLKWHSITILLILIRFSNTFTTAKKKEAFTETMGRMIGWWSLWVSKPKIKN"
     gene            complement(11494..>12054)
                     /locus_tag="PLQ70_03890"
     CDS             complement(11494..>12054)
                     /locus_tag="PLQ70_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQE33789.1"
                     /translation="NRFDGSLLSNIFSKIEQYGNPVMTTAYPQKSEIKKHQKVVQWST
                     FGAGNSFVRRDALENIRFNKAYEFGYGEDGDFGMQLRNQGFDVLYLPHPEILHLKAPM
                     GGFRTKPVLQWQNDKIQPKPSPTIMLYQLLHHSAAQRNSYKTTLFFKYYRLQSIKNPF
                     KYFAHFQKQWDRSVFWANELRKQNEI"
BASE COUNT         4269 a         2362 c         2054 g         3369 t
ORIGIN      
        1 ctctttgtgt ttttcttttc gaattatgct ttttatgagc acgagcaata cattacaact
       61 ggaattcatc aaagaattgg gttttatttg cattatattt ctctttttat aaccttgttt
      121 ttcggatata aattatacca aatcaacaag gaaaatccag ttttcaacct attcaaaaac
      181 aaattcgcag tttggtttgc agccttttta atcatttatc tggctagtac cgaattgatg
      241 ctgcacggat tggtcttatc gaattcacca ataacagctc aacaagtaca atcgaatgaa
      301 tattacaaaa attacaatgc aacagaattg ccctttctta aagcacaaat agcggacgat
      361 gccatacaat ctactcgaac ccaaatcata aaaactggtt ttccaattct atggggaatt
      421 ctcgcttttg ctttcttgat tattggaatt aaaaaacgaa ttaaagccat tagaatcata
      481 gccttatctt tattgggaat taccattctg aaattatttt tgttcgacat cagcaatgct
      541 tctgaaacag gcaaaatcat cgcttttatt ttgctaggag ttttaattct aatcatttca
      601 tttgtctatc aaaaaattaa agtcttggtg caagacgaca acaaacccaa taaaaccgat
      661 gagactgaat aattttttaa tatttctact agccgcaaat ggcctgttgg cacaacacac
      721 aacgacggca aaaatcgaaa ttccgaaaga aaacggcttg cacaaaatgg tgttacctgc
      781 cgaaattcgc tcgttttcaa aagaagaatt gggcgatttc agaatagtag attctaaaga
      841 aatagaagtg ccttattatg ttattcccca agaatatata gacaagacac ctcatttttt
      901 ttcggaatgc aaaataattt ctacaaacgt gcttcctaaa aataaaacaa cccttatttt
      961 cgaaaatccc aaaacgtcta tcgattcaat tgtactttgt ataactaatt ccgatgttac
     1021 taaaacctac tccattagcg gtagcaacga ccaaaaagaa tggtttggat tggtcaataa
     1081 ttcaaaatta tccgatctcg taaacacaga agaaacagtt gttttcaaaa caataagctt
     1141 tccattaact tcctatcgtt atatcaaatt tgattttaat gacaaaaaaa ctttgcccat
     1201 aaacattttg aaaatcggat tttttgccag caaaacaaca tcgaatattt tagaggaaat
     1261 tcatccaaaa aacatcaaat tccaacaaat tccagccgag aagaaaaccc gaatttacat
     1321 tagttttgat catccacaaa ttgtaaacca aatacatttc aaaatctcta gtcccaattt
     1381 atttcttcga aatacccaaa tatacgtgaa caaaaaaaaa cgcatcaacc aaaaagcagt
     1441 tgcgtttccg gaaaatttct cctcttttga attgaattcg aataccaaaa acacctttac
     1501 tattccacaa ttttttggaa aagaatgctt tatcgaaata gaaaatcagg acaatcctcc
     1561 cttgactttt tctgaaattc gcttttttca aaaaccaatt tcagtaattg ccgatttgaa
     1621 agccaatcaa aaatacactg tcgaaactgg aaattcaaaa ctgaatagtc ctgattatga
     1681 cttggaaaat ttcaagaaca ggatgggtag taacctgcct gaagccaaaa tatatgacat
     1741 caaacacgat atttctcaaa aaacaaatgc tctaaaaaaa tcattctggc aacaatcttg
     1801 gtttatgtgg atttgcatcg gtttaggtgg aattgtcatt gtctttttca caatgagttt
     1861 agtcaaggat atgaagaata attcaaccat ctaaactaat aattccctat ttttgtccaa
     1921 acattaacat aaaaacagga tatatgctca tcataggaat tgcgggtgga acaggctcag
     1981 gaaaaacaac agttgtccat cagataatga atgaattacc tcataccgaa gtgggaatta
     2041 tcgcccaaga ctcctactac aaagcctcta cagtcgaaag ctacgaagaa cgttccaaaa
     2101 ccaattttga ccatcccaga gccatcgatt tcgatttatt ggtcagccat ctcaaggaac
     2161 tcaaagaagg aaaaaccatc catcagcctg tttattcttt tgctattcac aacagaaccg
     2221 acgacaccat tgtcacacat cctcgtaaag taatgattgt agaaggaatt ttaatcctat
     2281 caaatcctga acttcgcgat ttattcgaca tcaaaatatt cgttcacgcc gattcagacg
     2341 aacgtttaat tcgaagatta aaacgggaca ttgccgagcg aggtcgcgat atggaagaag
     2401 tattaaacag ataccaaacc acactaaaac ctatgcacca gcagtttata gaaaacacca
     2461 aagcctatgc cgacatcatc attcccaacg acaaatacaa tacagtcgcc attgatgtgg
     2521 ttcgagctgt aataaatcaa cgaatcttat aatttttttt gtatttttat aactaaattt
     2581 tcaagaagct atttcccgct atccgctata tctcttgtgg cgaacaccgc caaaagaggt
     2641 aattgcgaaa gcaattgaat atcggggcta aaaaaaacag tatcacaaat ggcaaaccca
     2701 tataaagaca aatcttggtt caaattttta agcaacaaat acgtttgggt gttactacta
     2761 ttttctattt ggatgatatt tttggataac tattcctatt ttggacatcg ggttttagac
     2821 aaacaaattg atgaactcga acaaaacaaa gaatactacc aacaagaaat caaaaaagac
     2881 caagaaaata taaaaaaact aaaaaaccca gaccaaaccg aaaaatacgc ccgcgaaaaa
     2941 tactatatga aaaaagacag cgaagatgtc tatatcattg aatttgaagg cgacaccata
     3001 gaaaaagacg tcaaaaaata gcccctaaaa aaaaacaaag tgaatttcat ttgaagatat
     3061 aaccacactc gtaatgaaaa atttattcga cgatttcgat ccagtttctt ccaaacaatg
     3121 gaaacaaaaa atccagttcg aactcaatgg tgccgattac aaccaaactt taatctggaa
     3181 ttctcccgaa gacatcaaag taaaaccttt ttatcacagt gacgattaca ctccaacttc
     3241 gggcataaat acaaaagcaa cgcagttcct aatttgccaa aatatcttcg tacacgatct
     3301 tgcaaaatca aatgctagag ctattgacag catcaatcgt ggtgccgaaa gtattcgatt
     3361 taccatcgaa gacgaaaaaa tagaggtagc aaaactattg caaaaccttc cattagatac
     3421 aatcactatt tatttcaacc tcagttttat ttccatccct tttattaaga aaattgaagc
     3481 tgtagccaaa gaaaataagg caaaaatata ctgcaacctc gaccctattg ggcaattagc
     3541 caaagaagga aattggtttg aaaccaaaga aaaaaacaat ttcgagacct taaatctact
     3601 ttcagaatcc atttcaaata tttcattact tagcattgac ggaggtttat accaaaatgc
     3661 tggagccaat atggtgcaac aaattgccta tagtttagct catgccaatg aatatttcaa
     3721 ccaaataaaa accataaatc aacctatagt ttttcagatt tcagttggaa ctaactactt
     3781 tttcgaaatt gcaaaactca gagccttgcg gttgattttc aatttgattg ccaaagaata
     3841 caaccacaaa tttgattgtc acatactagt ttcacccaca aaacgcaaca aaacaatcta
     3901 tgattacaac gtcaatatgt tgcgaaccac aaccgaatgt atgagtgcaa ttttgggtgg
     3961 tgccgatgcc attgccaatt taccttatga cgccttgttt cacaaagaca acgaatttgg
     4021 caacagaata gcccgaaatc agttattagt gctcaaacac gaaagccatt ttgacaaagt
     4081 caacaatcca gccgatggaa gttattacat agaaagtctt accacacaat tagccgaaaa
     4141 agctttaact ttattcaaag ctattgaagc caatggcggt tttatcaaac aacttaacga
     4201 aggaattatc aaaagaaaaa tacaagaaag tgccgacaaa gaacaggaat tatttgattc
     4261 tggaaaagaa accttagttg gaaccaacaa atatcccaac aaaaaagacc gaatgaagga
     4321 cgatttagaa ttgtttcctt tcgtaaaaat aaaaccccga aaaacattga tttcgcccat
     4381 aatcgaaaaa cgattgtccg aaaaaatgga acaagaacga ctcaaagaag aaatataaaa
     4441 acaacatgaa aaacatactc tatacattag ccctactcct ttctctaacc gttttttctc
     4501 agcaaaaaac gatagaaata accaatatgg agtccggaaa aacagttgtt tttaaagaaa
     4561 accaacgcgt caaaatcaga acattagacc tcaaaaaatg ggtgggtaat cttaaatttt
     4621 cagacagtct ccatattatt gtaaacaatc ataaacttgc aattgacagc cttcaaagca
     4681 taaaaaacca gcccaaagta ctgggaacag tcaaaaccgt tgtcttaatt tcaggactag
     4741 caattgtagg cacttctcta attgcggcat caggcggaag tgaatctgca ttactaattt
     4801 ttatgattgg ttctggcaca accattagcg ctggaattat ggaaggtcta aataagaact
     4861 ataccaaaag aaaatggatg tataaagttg ttgaaaagtg atgcgtaaaa atctccaaca
     4921 tataaaatta gaaaagtcag aagttagaag tcagaagtca gaagtttcaa gaaaagacaa
     4981 cttcctcaca gccgaaggca tcgaactaaa accaacctat tctgaaaaag acatcgaaaa
     5041 cctcgaacat ctagattttg gagcgggttt ttcacccaat ttacgaggtc cttacgccac
     5101 gatgtatgtt caacgtccct ggaccattcg gcaatatgca ggtttttcaa cagcccaaga
     5161 aagcaatgct ttttatcgta gaaacttggc tgcaggacaa aaagggcttt ctatagcctt
     5221 tgatttaccc acccatcgag gttatgattc tgaccacgaa cgcgtggtag gcgatgttgg
     5281 aaaagcgggt gttgccatcg attcggtcga agatatgaaa ctattatttg accaaatttc
     5341 actagacgaa atgtccgttt ctatgacgat gaacggagcc gttttgccta ttatggcttt
     5401 ttatatcgtc gctgccgaag aacagggagt caacccagaa caactttcgg gaaccatcca
     5461 gaatgacatt ctaaaagagt ttatggtgcg aaatacctac atctatccac caacaccttc
     5521 gatgaaaatt attgccgata tatttgaatt cactagcaag aaaatgccca aattcaattc
     5581 aatttccatc tctggctatc atatgcaaga agctggagcc actgctgata ttgagttggc
     5641 ctacacatta gccgatggtt tagaatacat tcgaacaggg ttagctgctg gaatgaaaat
     5701 cgatgatttt acacctcgcc tttccttttt ctgggctatc gggatgaacc attttatgga
     5761 aattgccaaa atgagagctg gacgaatgat ttgggcaaaa atagtcaaac aattcaaccc
     5821 taaagacgaa aaatctctag ccttgagaac ccattgccaa acttcaggtt ggagtttgac
     5881 tatgcaagac ccctttaaca atgtagctcg aaccactatc gaagctgccg ctgccgcttt
     5941 tggcggaacc caatctttgc acaccaatgc cttggacgaa gccatcgctt tgccaaccga
     6001 tttctcggcc agaattgccc gaaataccca aatttatttg caggaagaaa ccaaaattac
     6061 caaaacagtc gatccttggg caggcagttt ttatgttgaa agcctaacga acgaaattgc
     6121 tcaaaacgct tggaaactaa tcgaagaaat agaagaattg ggcggaatga ccaaagctat
     6181 tgaatcgggt attcctaaat tgcgcatcga agaagccgca gccaaaaaac aagcgcgaat
     6241 tgatagcaat caagacctaa ttgtaggtat caacaaatac cgcttgaaaa aagaagaccc
     6301 actgcaaatt ctggatgtcg ataaccaaat ggtgcgccaa cagcaaatcg aacaattaga
     6361 aaaaattaaa tccagacgca ataccgaaaa agtacaactt tcgctcaaaa aactaaccct
     6421 ttgtgctcaa actggagaag gtaatttact agaaatcgct atcgaagcag cgcgaaatcg
     6481 agccacttta ggcgaaatta gtaatgcctt agaaacggtt ttcggaagat acaaagcaca
     6541 aattaaatcc tttagtggcg tgtatagcaa agaaataaaa gaagacaaaa gctttgagaa
     6601 agccaaaaaa atggccgacg aatttgccca aaaagaaggc cgtcgcccaa gaatcatgat
     6661 tgctaaaatg ggacaagatg gacacgaccg aggcgccaaa gttgttgcca caggttatgc
     6721 cgatttaggc tttgatgtcg atataggccc cttgtttcaa accccagccg aagccgccaa
     6781 acaagctgta gaaaatgatg tgcatatcct tggcgtttcc tctcttgcgg caggtcacaa
     6841 aacattagta ccgcaagtta tcgatgaact caaaaaatac ggtcgccaag acattatggt
     6901 aattgtaggc ggtgtaatcc ctactcaaga ttatcaattt ctgtttgatg ctggagcagt
     6961 tgccgtattt ggacctggaa ccaaaataag tgagagcgca attaaaatgt tggagatatt
     7021 attaatctaa agattaaact tcaaagactt taaaaacctt tgaggtttaa atacaaacac
     7081 aaaatatgtc caaactcccc aacgtaacca ccagcatttt tgccacaatg tctaaaatgg
     7141 caacagaaaa caatgcgata aatttggctc aaggatttcc aaattttgaa gtagatgata
     7201 aactggctca aattgtagca gcattggccc aaaaagaggt tcatcaatat ttgccaattg
     7261 ccggttatcc gcctctttta accaaaatcg ctaaactaat atttgattct tatcagcgaa
     7321 aagtcttgcc tgaaaccgaa atcctagtta caactggagc cactcaagca ctttttacaa
     7381 caattttggc attaataaaa acggatgacg aagttatcat tcttgatcca agtttcgata
     7441 attacgaagc acctactcta ttgtgcaatg ccaaaacgat tcgggtggaa ttgaatgacg
     7501 attattcgcc aaattgggag ctaattgaaa aggcaattac acccaagacc cgaatgttga
     7561 tcatcaatag cccacacaac ccaacaggga aaatttggaa agaaaatgat tttcaatctt
     7621 tagaaaatct attcgaaaaa tatccaaacc tattattgtt atctgatgaa gtctatgaat
     7681 tcatttcatt tgaacaaaaa cacagttcca tacattcaag aaaaaaaata gcaaacagga
     7741 gtattgttgt ttcctctttt ggaaaatctt tccatattac gggctggaga attggctatc
     7801 tggtagcacc agaatactta acaaaggaaa ttaaaaaagt acatcaatac ttagtctttt
     7861 gtgtaaatag tcttgcccaa gcggcaataa gcgactatct agaagtggcc gatgtaacaa
     7921 aactggggaa cttctaccaa gaaaaacgag accattttag agcattactc caaaacagta
     7981 gatttgaact gttgccctgc gaaggaacct atttccaagt ggtgtcttat gcttccattt
     8041 caaatgaaaa tgatgtgaat ttttgcaaga gattaattac tgaatatggt gttgcagcca
     8101 ttcctatttc agctttttat gccgacggaa aagaccaaaa gctcattcgg ttttgctttg
     8161 ccaaagacaa taaaacattg gaagaagcgg ctagacgatt gtgcaaagtg tgatgctaaa
     8221 ccagtcaggt tatagaaaca agtcaggttt aattccttat atttacatta ctaaaaaata
     8281 aaactatgga tttctcagca aaaatgcttc gtgacgatgc tctaaaagga aaagtgattg
     8341 tagtaacggg tggcggaagc ggattaggca aagccatgac ccgctatttt atggaattgg
     8401 gagccaaagt agccatcagt tcccgtgatt tagaaaaact gcaaaacaca gccaaggaat
     8461 tggaaactga aactggtgga acttgtcttg ctgtgcaatg tgatgttcgt cattatgaag
     8521 aagtcgaaaa tatgctggaa gaagtcttga aaacgtatgg aaaaatagat gtattactca
     8581 acaatgccgc gggaaatttc atttcaccaa ccgaaagatt atcggccaac gcttttgaca
     8641 ccgtgattga cattgttttg aaagggtcta aaaactgcac tcttgctttt ggaaaacatt
     8701 ggattgactc caaacaaacc cacgcaacta tattaaatat tgtgaccact tatgctttta
     8761 caggttcggc ttatgtcgtt ccaagtgctg cagcaaaagc tggtgttttg gctatgactc
     8821 gaagtcttgc tgtagaatgg gcgaaatatg gcatccgaac caatgccatt gctccaggcc
     8881 ctttccctac taaaggcgct tgggatagat tgttacctgg cgacttggca gaaaaatttg
     8941 atatggctaa aaaagtacct ttgaaacgag tgggtaatca tcaggaatta gcgaacctag
     9001 cagcttattt agtttctgat ttctcagcct acattaatgg cgaagtgatt gtgattgatg
     9061 gtggcgaatg gctgaaaggg gcaggtgaat tcaatattct cgaagccatt cccgaagaac
     9121 tttgggatca attggaaatg atgattaagg ccaagaaacg aagttagcag tgttcggttt
     9181 tcagtactcc taaatctggg aaaatcattc aacttgggag tttttacttg gcgtatttcc
     9241 attaaaataa tagttcaaat aatacccaaa caagaacacc attatgggac gcaccggaaa
     9301 catataacgc tgctcagaat gaaagataaa aacatacact aaagaccagc cccaaaacaa
     9361 tagaaaaggc atcattaatt ggtacttttt ttgtttgttc ataaccacaa tagctgcgat
     9421 agaaaagaaa attaagagat aattaatggc gttgatgtaa atgtgaatgc tccattttac
     9481 aaaattactc ttattgtatt tcattatggg cgaaatgaca aaagtctgag attgaaagaa
     9541 tttcaagctg tactgcctca aagtcaatcc cggattttga ataatatcat tggttaccca
     9601 cttgatgtaa taatctgttt tatcaaaatg attgacccga caaatagaat ccaactcatc
     9661 cctctttgcc aagttatgga ggtaatcgga agtagcaaac tctttttgcc cgtcttgcgg
     9721 caaccaattg aaaggatcat ctcttaattc ataacgagac caaagaagat gccttctgaa
     9781 aatatctttt ttgaaagcac catcggtatt attgattttt ttttcgacca aagccaaaaa
     9841 gagcaacatt aaccctagtg acgaaataaa aatcactttg tttttccatt ctgattttat
     9901 ggttaaaaaa tacaaagtaa acaatactaa aaacggtaat aaattgggtc tagtaccaga
     9961 gacgattacc aaagaaaaag aaagcatcaa ataatattta accgcattcg aattagctat
    10021 aatttttaac caagcataaa taaagagtga aaccccaaaa aaagcagcac tttcagccag
    10081 aattcccatg gcataataaa catgtatcgg gaacaaattt aaaacgatga tagtccaaaa
    10141 cttagttcgg gtaggaaacg acattaggtc aaaagaagca aatatatatt ggattgccca
    10201 acaagtaata atcgaattat aagcaactcc caaaagataa aatagagtat cattatggaa
    10261 aacatacccc aaagaataag gcaccaaata ataaaacaac gtaaacaatc ctttgtcaat
    10321 acgtacagtt ccttgaaatc cgtttaatat gtattggatt agttgaatgg ttccttcgtg
    10381 gtattctcca tcgccaaaag gtttgggttt aaaaggtaaa aaataaaaca ccacacacac
    10441 taaggcagta attaaaacag gatatttagc aacaaaatca attactcttt tcataatgaa
    10501 attcaattct ttattttagg tttacttacc cacaaactcc accaacctat cattctcccc
    10561 attgtttcgg taaaagcttc cttctttttg gcggtggtaa atgtattaga aaatcgtatc
    10621 aaaatcaaca atatcgtgat ggaatgccat ttcagttttg cttttaaaga gggggttgga
    10681 ttcttagctc tccaaacata ccagccattt ctaaccacca ttttaccata tttatactga
    10741 ttaggtcttc ccgaaggatg atgaaaatga ctcaattggg ctttggtatt gatggcattt
    10801 tttccaaatt gcaaagctcg gatactaaaa tcggcatctt catacaagcc atagccttca
    10861 aaataggatg aaaatctaat agtatctaga accttttttc gaaaagaaaa agacattcca
    10921 atcaacaaat ctacctcata ggtttttcca ttcaagggaa atccacaagt tcgaccatga
    10981 gaataatcag gcattcgccc tggtcctaaa tttgattgca atcctaaata attccgaacc
    11041 acattgcgct gcccttcttt gtaaacaaat ccatctaatg gataatagcg gtttggattg
    11101 tatgttttat tcggctctgc caattgccaa ccgttttcat tgatggcgac tccagccaca
    11161 cccgaaatag ctggattttc ttcaaaggtt tgaattaatt ccttgaaata atccgcttct
    11221 aaaatcgtat catcatccaa aaaagcaatg acttccattg agcttcccgc tttttgaact
    11281 ccaaaatttc tttgtctagt caaacctcta tattctggtg gaacagcaaa ataatgcaaa
    11341 ttctgaaatg gatgttcttt taaagccaat tctgtttctt gattggtcga accatcgata
    11401 atcaggattt cgtcaggata taaagtttgc ccctgaaccg attgcagcaa ttgcaacacc
    11461 gattgaggac gcatataagt gcaaatgatg agactaaatt tcattttgtt tccttaattc
    11521 gttagcccaa aacacacttc tgtcccattg tttttgaaaa tgagcgaaat atttaaatgg
    11581 attttttatc gattgcaatc tataatactt aaaaaacaag gtggttttat aactatttct
    11641 ttgggctgcc gaatgatgca aaagttgata cagcattatc gtcggagagg gcttcggttg
    11701 aatcttgtcg ttttgccatt gcaagacagg tttagttcta aaaccgccca ttggtgcttt
    11761 caaatgcaag atttcagggt ggggtaaata caaaacatca aaaccttgat tgcgcaattg
    11821 cattccaaaa tcgccgtctt cgccataccc aaattcatat gctttattga atcgtatgtt
    11881 ttccaaagca tcccttctaa caaaactatt tcctgcccca aaagtggacc attgtactac
    11941 tttttgatgt tttttaattt cgcttttttg aggatatgct gttgtcatta ctggatttcc
    12001 atattgttca attttactaa aaatattaga taataaagaa ccatcaaaac gatt
//
LOCUS       DAOSNN010000046        11986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5289_length_11986_cov_5.564915, whole genome shotgun sequence.
ACCESSION   DAOSNN010000046 DAOSNN010000000
VERSION     DAOSNN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11986
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5289_length_11986_cov_5.564915"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..770)
                     /locus_tag="PLQ70_03895"
     CDS             complement(48..770)
                     /locus_tag="PLQ70_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HQE33790.1"
                     /translation="MKLFNWANIRLILMFSLVIFLYSFTSNRNNSRKLTKSTVVFVGD
                     TAPYITSETVNKLLIENNKDVKSIGKDKLDLNKLERELNANKMIEKSDVFVSIDGELK
                     AVVKQKTPIARIFDGEKSFYIDYKGNTMPLSSNYTARVPLVSGEINKKNSKKLAELFR
                     IIYDDEFLKKNIIGVQIMPNGSIKMMNRNYDYQIDFGGTLRMKAKFNNYKAFFQKAVL
                     DSSLNKYKTIDLRFAQQVVCTK"
     gene            complement(757..2106)
                     /gene="murC"
                     /locus_tag="PLQ70_03900"
     CDS             complement(757..2106)
                     /gene="murC"
                     /locus_tag="PLQ70_03900"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQE33791.1"
                     /translation="MNLNQIHNVYFIGIGGIGMSALARYFKAIGKNVSGYDKTETELT
                     KDLSQLGIDIHFEDRIDLIPTDYYAENTLVIITPAVPKSHSEWNYFLERNYELKKRAE
                     VLGIITKDTFCFAVAGTHGKTTTSSILGHILFESGVDVTAFVGGIVENYNSNLIGNGK
                     TITVVEADEFDRSFLHLHPDIACVTSMDADHLDIYGDKTAIEASFTEFADKIEDKSKL
                     FITTELPIKGLTCAVNEEANFTAFNIRIINSQYVFDVKTPTETLYNIEFGLPGKHNLM
                     NALMALAMAKTFGLPTEDIASALRSFKGIKRRFSYQIKNERLVYIDDYAHHPTEINAV
                     HQAVRELYPNQKVLAVFQPHLFSRTKDFADEFAKSLSAFDEVILLDIYPARELPMEGI
                     TSSWLIDKVENLNKKLTTKENLIPSILESNATVIVTIGAGDIGEMVTTIKNSLNETV"
     gene            complement(2302..3390)
                     /gene="murG"
                     /locus_tag="PLQ70_03905"
     CDS             complement(2302..3390)
                     /gene="murG"
                     /locus_tag="PLQ70_03905"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQE33792.1"
                     /translation="MKKLKFILSGGGTGGHIYPAVAIANELKSRFPTAEFLFVGAKDK
                     MEMQKVPQAGYEIKGLWIAGLQRKLTLQNAMFPFKLIDSLWKSRTIIKNFKPDVVIGT
                     GGFASGPLLQMANMLHIPTLIQEQNSYPGITNKLLSKKAHKICVAYENLEHFFPKEKM
                     ILTGNPVRQDLIDIESKKNQAIQHFNLDANKKTVLILGGSLGARRINQLIEKELANFK
                     TLDVQVIWQCGKLYFEDYKKYKSNTVQVLDFIDRMDLVYAAADIVISRAGASSVSELC
                     IVGKPVIFIPSPNVAEDHQTKNAKSIVDKKGALMLKESELESQFSIVFEALLKDSGKQ
                     ELLSKNIKQLALPNATKQIVDEIVKLIK"
     gene            complement(3425..4741)
                     /locus_tag="PLQ70_03910"
     CDS             complement(3425..4741)
                     /locus_tag="PLQ70_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQE33793.1"
                     /translation="MKELINNLKGDKVIWTFVALLALFSFMPVFSASSNLAYIGQGTG
                     NTISYLLKHLAHIFIGLLIIFGIHKVPFHYYRGISLFLLPVVWILLAITMIKGTVIAG
                     ANASRWIQIPFIGVSFQTSTVAAIVLFIFVARYLSKKREEEIDFKTSLIELWLPVFIT
                     LALILPANFSTASLIFLMVLMLTFVGQYPLKYLAYIIGFGIAALLLFILLAKAFPESR
                     LFSRVETWKSRVENFSKNKPGEDDYQIEKAKIAIASGGIYGLGPGKSVQKHFLPQSSS
                     DFIYAIIVEEYGLIGGFGVLLLYLLLFFRFVIAAHKANTVFGKLLVVGLGFPMIFQAM
                     INMCVAVELLPVTGQTLPLISSGGSSIWMTCIALGIIINVTKKEEEIAQEISDKAKRD
                     AALQKLIDKQLEDENNEENSISTDAETENYSIEDNSANPMNAVLNK"
     gene            complement(4818..6155)
                     /gene="murD"
                     /locus_tag="PLQ70_03915"
     CDS             complement(4818..6155)
                     /gene="murD"
                     /locus_tag="PLQ70_03915"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQE33794.1"
                     /translation="MRLVVLGGGESGVGTAILGKKKGYDVFVSDFGKIKDKYKKVLID
                     NDIAWEQEKHTEELILNANVVMKSPGIPEKKSAIVKKLIEKGIPILSEIEFAAPFTKA
                     ITIGITGSNGKTTTTMLTYHLLKSAGLNVALGGNIGKSFAWQVAEDKFDSYVLELSSF
                     QLDGIIDYKPHIAIITNISPDHLDRYDYKYEKYIDSKFRITMNQTEEDYLIYDADDEA
                     IAEWLKNNKTRAQLIPFSLTKTFEIGAFIKEKTMEVNIINEEEFTMETETMALEGKHN
                     MKNAMAATSVAKLMKIRNATIRESLSNFQGVEHRLEKVLKIQNVQYINDSKATNVNAT
                     FFALDSMNTPTVWIVGGVDKGNDYNELMSLVREKVKAIICLGVDNRKIIDVFGNVVDM
                     MIEVNNMEDAVKMAQRLSEKGDTVLLSPACASFDLFENYEDRGNQFKQAVKHL"
     gene            complement(6155..7384)
                     /gene="mraY"
                     /locus_tag="PLQ70_03920"
     CDS             complement(6155..7384)
                     /gene="mraY"
                     /locus_tag="PLQ70_03920"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQE33795.1"
                     /translation="MLYYLFEYLDKTIDFPGAGVFQYITFRSALAFMLSLLLSTIYGK
                     RVVGFLRKQQIGESVRELGLAGQNEKAGTPTMGGLIIIFATLIPVFLFAKLQNIYIVL
                     LVVTTLWMGTIGFIDDYIKIFKKDKEGLKGIFKVFGQVGLGLIVGSILYFNPMVTIRT
                     DTPKSEISRTVSENVIKQAPVYEKSTATTIPFFKNNSFDYAEILAWTGDGYEKWAWLI
                     FIPMVIFIITAVSNGANLTDGIDGLAAGTSAVSVLALGIFTFVSGNMIFSNYLNIMYI
                     PYSGEMTVFISAFVGALIGFLWYNSYPATVFMGDTGSLTIGGVIAVLAIAVRKELLIP
                     LLCGIFLVENFSVVLQVAYFKYTKKRFGEGRRVFLMAPLHHHYQKKGYHESKIVTRFW
                     IVAIMLAILSIVTLKLR"
     gene            complement(7391..8854)
                     /locus_tag="PLQ70_03925"
     CDS             complement(7391..8854)
                     /locus_tag="PLQ70_03925"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQE33796.1"
                     /translation="MIVLKEIIYKVAIEAVKGSTDIAINKLDFDSRKMEANDVFVAIR
                     GSISDGHDYIEKAIHLGAIAVVCDTFPENPISGITYIQVKDTNKALAFMAANYFGNPS
                     SNLKLVGITGTNGKTTIASLLYQLFKKAGFKVGLLSTVKILVDTIEYKATHTTPDSIT
                     INHYLKEMTEAGVEYCFMEVSSHGIHQKRTEALHFAGGIFTNLSHDHLDYHPTFAEYR
                     DVKKSFFDNLPKTAFALSNIDDKNGAVMLQNTAAKKLTYALKSYADYKAQILENQLSG
                     LLLKINGNEVWVKLIGTFNAYNLLAIYGTAIELGLENLEVLRLLSDLESVSGRFQFIV
                     SNSNITAIVDYAHTPDALENVLKTINDIRTKNEQLITVVGCGGNRDKAKRPIMANIAT
                     ELSDKTILTSDNPRNEIPEEIINEMEQGVAAQNYKKSLSITDRKQAIKTACQLAQPND
                     IILIAGKGHETYQEINGVRHHFDDMETVTELLEKLNK"
     gene            complement(8851..10857)
                     /locus_tag="PLQ70_03930"
     CDS             complement(8851..10857)
                     /locus_tag="PLQ70_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HQE33797.1"
                     /translation="MAVEDKKISYRIYIIATIIFMLALAIAVKLTNIQWVEGDYYRKL
                     AKDRTVKNFVLPANKGNIYSADGSLLATSIPNYEIRFDAVAPKAEAFDKNVKSLADSL
                     SLLLSKPSYFYLNELRKARANKNRYYLIARDLSYTEFMKIKGFPLFKLGPFKGGIIVE
                     QETVREHPIGEIAERTIGYERKNPNGMPDGKGIEWAYRKYLNGKDGKVLKQKIAKGQW
                     KPISDANQIDPQDGYDVISTIDVYIQDIAHHALLKQLELYQADHGCVVVMETKTGKIK
                     AISNLGRHDDGSYFETTNYAYTESHEPGSTFKLVDLMILLEDKKIDTSAVFNTYGGII
                     KYSGKSVRDSHEGGYGKISLARGFEVSSNTVMVQAVYNNYKNNPSQFVNHINSFGLNK
                     KLGLEFKGEGKPFIPQPSDKNWSNLSLPWMAFGYGVSVTPLQTLTFYNAVANNGEMVK
                     PQIVSEIKQWDTSIKKYDKVVMNPKICSKETILKLKAVLQNVVKKGTGKGLYSKDFSM
                     AGKTGTAQVNYAKNGGSEKYYASSFVGYFPAESPKYSCIVVVHKPSTINNNYYGADVA
                     GPVFKRIAQKIFTDAPSTNEIKNIDRKIQKQEKEYDAFYTKSQKQQPSKIPNVKGMSG
                     MDAVALFGNLGMKVKIIGIGKVKKQSIQAGEPLEKNTTITLELS"
     gene            complement(10848..11198)
                     /locus_tag="PLQ70_03935"
     CDS             complement(10848..11198)
                     /locus_tag="PLQ70_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HQE33798.1"
                     /translation="MKNGVYSILKAKFLIEDDSMKNWRFIIFLIVLAIIMIANTQRFE
                     QKIFKIAELNNKVKELRSEFVDRRSELMKLKMESTVSAKVAEKQIFPSTVPPTKIKVK
                     KVIEDKNFFQKIWQ"
     gene            complement(11268..>11986)
                     /gene="rsmH"
                     /locus_tag="PLQ70_03940"
     CDS             complement(11268..>11986)
                     /gene="rsmH"
                     /locus_tag="PLQ70_03940"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQE33799.1"
                     /translation="ALADERFTLINENFRFIKRFLRFYGVKSVDGILADLGVSSHQFD
                     VAERGFSTRFDAELDMRMSQKNDLNAYRIVNEYDDINLKRVFLDYGELKNAPALARTI
                     VEAREHHPIKTTDELKDVLAKYLPERVRNKILAQIYQAIRIEVNQEMDVLKEFLEQSL
                     EILNPGGRLSVISYHSLEDRLVKRFMKNGMFEGEPERDFFGNFSVPFKTIGKLIVPDD
                     AEIKTNNRARSAKLRIAEKI"
BASE COUNT         3524 a         2161 c         1891 g         4410 t
ORIGIN      
        1 gtcccaatat ctagacctac tgcaatattc tctttttcca ttatctatta tttagtacat
       61 actacttgct gagcaaatct taggtcgatt gttttgtatt tatttaatga gctatctaaa
      121 actgctttct gaaaaaaagc tttgtaatta ttaaattttg ctttcattct taatgtccca
      181 ccaaaatcaa tctgatagtc gtaattcctg ttcatcattt taatgcttcc atttggcata
      241 atctgaacac caatgatgtt ttttttcaaa aactcatcgt catatataat ccgaaataat
      301 tcggctaatt ttttgctatt ttttttattt atttcccctg aaacaagcgg aactctagcc
      361 gtataatttg atgataaagg cattgtattt cctttataat caatatagaa tgacttttct
      421 ccgtcaaaaa tcctggctat tggagttttt tgtttcacaa ctgcttttaa ctcgccatcg
      481 atgctcacaa acacatcaga tttttcaatc attttgtttg catttagctc tctttccagt
      541 ttattcaaat ctaatttatc tttgcctata ctttttacgt ctttattatt ttctattaac
      601 aatttattaa ccgtttctga agtaatatat ggagcggtat ctcctacaaa aacaacggtt
      661 gattttgtca attttctgct attatttcga tttgaagtaa acgaatacag aaaaatcacc
      721 aatgaaaaca tcagtattaa tcgaatattt gcccaattaa acagtttcat ttaacgaatt
      781 tttaattgtt gttaccattt ctccaatatc tcctgcacca attgttacta taacggttgc
      841 gttactttct aaaattgatg gaatcaaatt ttcttttgta gttaatttct tgttcaaatt
      901 ttctacttta tcgattagcc aactggatgt aattccttcc attggcagtt ctcgtgctgg
      961 ataaatatcc aataaaatca cttcatcaaa agctgacaaa cttttggcaa attcatcggc
     1021 aaaatctttg gttctgctaa acaaatgagg ttgaaaaact gctaaaactt tttggtttgg
     1081 atacaattct ctaacggctt gatgaaccgc atttatttcg gttggatgat gagcataatc
     1141 atcgatataa actaatcttt cgtttttaat ttgataggag aatcttcttt ttattccttt
     1201 aaaagaacgc aaagcactag caatgtcttc ggttgggagg ccaaaggttt ttgccattgc
     1261 caatgccatt agcgcgttca ttaaattgtg ttttcctggc aaaccaaatt caatattgta
     1321 taatgtttct gttggtgttt ttacatcaaa aacatattga ctgtttataa ttctgatatt
     1381 gaaagcggtg aaatttgcct cttcatttac tgcacaagta agacctttta ttggcaactc
     1441 ggttgttatg aaaagtttgg atttatcttc aattttatct gcaaattctg taaacgaagc
     1501 ttcgattgct gttttgtcgc catatatatc taaatgatct gcatccattg aggttacaca
     1561 agcaatatcg ggatgtaggt gcaaaaagga gcggtcaaat tcgtcggctt ctacaacagt
     1621 tattgttttt ccgtttccaa ttaaattaga attataattt tcgacaattc caccaacaaa
     1681 agccgttaca tcgacaccac tttcgaacaa tatatgtcca agaatgcttg atgtagtggt
     1741 ttttccgtga gttccagcca ctgcaaaaca aaaggtgtct tttgtaatga taccaagaac
     1801 ttcggctctt tttttcaatt cataatttcg ttctaagaaa taattccatt ctgaatgtga
     1861 ttttggaacc gctggtgtga taatcacaag ggtgttttct gcgtaataat cagttggaat
     1921 taaatcaatt ctatcttcaa aatgaatgtc aattcctagt tgagaaaggt cttttgtaag
     1981 ttcggtttct gttttgtcat aaccagatac atttttccca atggctttga aataacgcgc
     2041 caaagcactc attccaattc ctccaattcc tataaaatag acgttatgta tttgatttag
     2101 attcatattt aatttaaaat atagttataa cattttttta ataaatcatc aaacaatatg
     2161 attccaataa ttattacaat taataaaatt atatttagta ttgatttctt aaaatatttt
     2221 ttatcctttt tataatcaaa atagtaagca attccaattg caattaatga caagaaaaat
     2281 aaaatcaata ttacattcat cttattttat caatttcact atttcgtcca caatttgttt
     2341 tgttgcgttt ggcaatgcta attgtttgat gtttttactc aacaattctt gtttcccact
     2401 gtctttcaaa agggcttcaa aaacgatgct gaattgcgat tctaattctg attcctttag
     2461 cattaatgct ccttttttat ccacaattga ttttgcgttt ttggtttgat gatcctcggc
     2521 aacattgggt gatggaataa aaatcacagg ttttccaacg atgcacaatt ctgaaaccga
     2581 tgaggctcca gctcgagaaa ttacaatatc ggcagcggca taaaccaaat ccattcgatc
     2641 aatgaaatct aaaacttgaa cggtatttga tttgtatttc ttataatctt caaagtataa
     2701 ttttccgcat tgccagatta cttgaacatc taaggttttg aaattagcta attctttttc
     2761 aattaattgg ttaattcttc ttgcgccaag gcttccaccg agaatcaaaa ccgttttttt
     2821 attggcatct aagttgaaat gttgtatggc ttgatttttt ttgctttcaa tgtcaattaa
     2881 atcttgacga actggatttc ctgttaaaat cattttctct tttggaaaaa aatgttctaa
     2941 gttttcgtaa gccacacata ttttatgggc tttcttgctc aataatttat tggttattcc
     3001 ggggtatgaa ttttgttcct gaattagtgt tggaatatgc aacatatttg ccatttgcaa
     3061 aagtgggcca gaagcaaagc ctccggttcc aattactaca tcgggtttaa agtttttgat
     3121 tattgttcta gatttccata aactgtctat taatttgaat ggaaacatag cgttttgcaa
     3181 ggtcaatttt cgttgtaaac ctgcaatcca aagacctttt atttcataac cagcttgagg
     3241 aactttttgc atttccattt tgtctttggc acctacaaaa agaaattcgg cagtaggaaa
     3301 tcgtgatttt agttcattgg caatcgcaac agcagggtag atgtgtccac cggttccgcc
     3361 accacttaat atgaatttta attttttcat ttttcctaaa atttaatggg aatacccaaa
     3421 atgattattt attcaaaacg gcattcattg gatttgctga attatcctcg atagaataat
     3481 tttcagtttc tgcatcagtg ctaatggagt tttcctcgtt attttcgtct tctaattgtt
     3541 tgtctattaa tttttgcaag gcagcatctc tttttgcttt gtcgcttatt tcttgtgcaa
     3601 tttcttcttc ttttttggtt acattgatga tgattcctaa ggcaatacac gtcatccaaa
     3661 tagaacttcc accactacta attaggggta atgtttgacc tgttacgggc aataattcta
     3721 ctgcaacaca catgttaatc atggcttgaa aaatcattgg aaatccgagt ccaactacta
     3781 aaagttttcc gaatacagta ttggctttgt gagcagcaat gacgaatcta aaaaacaata
     3841 ataaatacaa aagcaagacg ccaaaaccac ctattaaacc gtattcttcg acgattatgg
     3901 cataaataaa atcggaagag gattgtggta aaaagtgttt ttgtacactt ttcccaggac
     3961 caagaccgta aattccacca gatgcaattg ctattttagc cttttctatt tgataatcat
     4021 cttcgccggg tttgtttttg ctgaaatttt ctaccctact tttccatgtt tccactctac
     4081 taaataatcg ggattctgga aatgcttttg ctagcaaaat gaaaagtaat aaggctgcaa
     4141 ttccaaaacc gattatatat gctaaatact ttaaaggata ttggccaaca aaagttagca
     4201 tcaaaaccat taggaatatc aaggacgcag tcgaaaaatt ggcaggtaga attaaggcta
     4261 atgttatgaa aacgggtagc caaagttcga ttaatgatgt tttaaaatcg atttcttctt
     4321 ctcgtttttt tgataaatag cgtgcaacaa atataaataa tacaattgct gctacagttg
     4381 aagtctggaa tgatacacca ataaaaggta tttgaatcca tctactggcg ttagcaccag
     4441 cgattactgt tccttttatc atagtaatgg ctaacaaaat ccaaactact ggaaggagaa
     4501 ataaagagat tcctctgtaa taatgaaacg gtactttgtg aattccaaag ataattagta
     4561 atccaataaa tatgtgggct aaatgtttta gtaaataact aatagtgttc cctgtacctt
     4621 gtccgatgta ggccaaatta ctactcgcac taaaaacagg cataaacgaa aacaaagcta
     4681 ataaagccac gaatgtccat attactttgt ctccttttaa attgtttatt agttctttca
     4741 tgttttgggt tttgggtttt gggttttagg tcttggggtt tttagaattg tccaacacct
     4801 aatgcctaaa acctatttta caaatgctta actgcttgtt taaattgatt gcctcggtct
     4861 tcgtagtttt cgaataaatc gaagctggcg caagctgggg ataacaatac cgtatctcct
     4921 ttttcagaca atctttgagc cattttcacg gcatcttcca tattgttaac ttcaatcatc
     4981 atatctacaa cgtttccaaa aacgtctata atttttctgt tgtcaacacc caaacagata
     5041 attgctttta ctttttcgcg aactaatgac attaattcat tgtaatcatt tcctttgtca
     5101 acacccccta caatccaaac cgttggggta ttcatactgt ctaaagcaaa gaaagttgcg
     5161 tttacattag tagcttttga atcgttgatg tattgtacat tttgaatttt caatactttt
     5221 tctaaacgat gttcaacccc ttgaaaattg gataaacttt ctctaatggt tgcatttcta
     5281 attttcatca attttgctac agaagttgct gccattgcat ttttcatatt atgctttcct
     5341 tcaagcgcca tagtttcggt ttccattgtg aattcttcct cgttgatgat gtttacttcc
     5401 attgtttttt cttttataaa ggctcctatt tcgaatgttt ttgttagcga aaaaggaatt
     5461 aattgagctc ttgttttatt gttttttaac cattctgcga ttgcttcgtc atctgcgtca
     5521 taaatgagat aatcttcttc agtttggttc attgtgattc taaatttaga atcaatatat
     5581 ttttcatatt tgtaatcata tcggtctaaa tgatctggac tgatattggt aattatagcg
     5641 atgtgtggtt tgtaatctat gattccgtcc aactgaaaac tacttaattc cagcacataa
     5701 ctatcaaatt tatcttcggc aacttgccaa gcaaaactct ttccgatatt tcctcctaaa
     5761 gcgacattca atcccgccga ttttagcaag tgataggtta gcattgttgt tgttgtcttg
     5821 ccgttacttc cagttattcc tattgtgatt gcttttgtaa agggtgctgc aaactcaatt
     5881 tctgaaagta ttgggatacc tttttcgatc aattttttta caattgccga ttttttttct
     5941 ggaataccag ggcttttcat caccacatta gcattcaaaa tcagttcttc tgtgtgcttt
     6001 tcttgctccc aagcaatatc gttatcgata agaacttttt tgtatttgtc ttttattttt
     6061 ccaaaatcag atacaaaaac gtcatatcct tttttcttac caagaattgc agttccaaca
     6121 ccactctcgc ctcctcctaa aactactaat ctcattatct taactttaag gttacgatgg
     6181 ataaaatggc tagcattatg gctacaatcc aaaaacgggt tacgatttta ctttcgtgat
     6241 agcctttctt ttgataatga tgatgcagtg gcgccatcaa aaacactctt cgtccttcgc
     6301 caaagcgttt tttggtgtat ttaaaatagg caacctgcaa aaccactgaa aaattttcga
     6361 ctaaaaatat tccacacaat agcggaatta gtaattcttt tcgaactgca atggctaaaa
     6421 cagcaataac accacctatg gttaagctcc ctgtgtcacc cataaaaact gttgctggat
     6481 aggaattgta ccaaagaaaa ccaattaatg ctccaacaaa tgctgaaata aatactgtca
     6541 tttcaccaga ataggggata tacataatat ttaagtaatt cgaaaaaatc atattacctg
     6601 aaacaaaagt gaaaataccc aaggctagca ccgaaactgc cgaggttcca gcagcaaggc
     6661 catctattcc gtcggttaga ttggctccgt tagataccgc tgtgataata aaaatgacca
     6721 tcggaataaa tattaaccaa gcccattttt cataaccatc tcctgtccag gctaatattt
     6781 cggcatagtc aaaagagttg ttcttgaaaa atggaatagt tgtagcagtt gatttttcgt
     6841 aaactggggc ttgttttata acattttctg aaactgttct tgagatttca ctttttggag
     6901 tatcagttct tatggtaacc atagggttga aatacagtat cgaaccaaca attaaaccta
     6961 aaccaacttg tccaaaaact ttgaaaatgc cttttaatcc ttctttgtct tttttgaata
     7021 ttttgatata gtcatctatg aaaccaattg ttcccatcca caaagtggtt acaactagca
     7081 acactatgta aatgttttgg agtttagcaa aaagaaaaac aggtattaaa gtggcaaaaa
     7141 taataatcaa tccacccatt gttggggttc ctgctttttc gttttgacct gctaaaccca
     7201 attcgcggac cgactccccg atttgttgtt ttcttaaaaa cccaaccact cttttaccat
     7261 aaatagtaga tagaagcaag gaaagcataa atgctaaagc cgatctaaag gtgatgtatt
     7321 gaaaaactcc cgctcctgga aagtcgattg ttttgtctaa atattcaaat aagtagtata
     7381 gcataattag ttatttgttg agtttttcta aaagttccgt tactgtttcc atatcgtcaa
     7441 aatgatgacg aactccatta atttcttgat aggtttcgtg cccttttcct gcaattaaga
     7501 taatgtcatt gggctgagcc aattgacaag ctgtttttat cgcttgtttt ctgtcggtta
     7561 ttgataatga ttttttgtaa ttttgagcag caaccccttg ttccatttcg ttgataattt
     7621 cctccggaat ttcatttctg ggattgtctg aagttaggat ggttttatca ctaagctcag
     7681 tagcgatgtt ggccattatt gggcgtttgg ccttgtctct gttgcctcca cagcctacaa
     7741 ctgttattaa ttgttcgttt ttagtacgaa tgtcattaat cgtttttagc acattttcca
     7801 aggcatcagg tgtatgagca taatctacaa tagcggtaat attcgaatta gaaactataa
     7861 attgaaaacg tcccgaaaca ctttctaaat cggataataa tcgaagtact tctaaatttt
     7921 ccaatcctaa ttcaatggct gttccgtaga ttgccaaaag attgtaggca ttgaatgtcc
     7981 caattaattt cacccaaact tcgtttccgt ttatttttaa taacaaaccc gacaattggt
     8041 tttctaagat ttgagcttta tagtcagcat atgattttag agcgtatgtt agtttttttg
     8101 cagcggtgtt ttgcaacatt actgctccat ttttgtcgtc gatattggat aaagcgaaag
     8161 ctgtttttgg taaattgtcg aaaaaagatt ttttcacatc acgatattca gcaaaagtgg
     8221 ggtggtaatc caaatgatcg tgtgataaat tggtgaatat tccacctgca aaatgcaagg
     8281 cctcggtgcg tttttgatga attccgtgcg aactcacttc cataaagcaa tattcaacac
     8341 cagcctctgt catttctttc aaataatgat tgattgtgat agaatcggga gtagtgtgag
     8401 ttgctttata ttcaatagta tcaaccaaaa tttttacggt tgaaagcaat cctactttga
     8461 aacctgcttt tttaaatagt tgatacaata atgaagcaat agttgttttg ccattagttc
     8521 cagtaatgcc tactaatttc aagtttgaag acggattgcc aaaataatta gctgccatga
     8581 aagccaatgc tttattagtg tcttttactt gaatataggt tatgccactt atggggtttt
     8641 ctggaaaagt atcacaaact acagcaatag cccccaaatg gattgctttt tctatatagt
     8701 cgtgaccgtc tgaaatgcta cctctaatgg ctacaaatac atcattcgct tccatttttc
     8761 tggaatcaaa atccaattta ttgatagcga tatctgtcga accttttaca gcttcgatag
     8821 cgactttata tattatttct tttaaaacaa tcacgataat tctagtgtta tagttgtgtt
     8881 tttttctaag ggttctccgg cttgaataga ttgttttttt actttaccaa tgccaattat
     8941 ttttactttc attcctaaat ttccaaataa agcaacagca tccattcctg acattccttt
     9001 tacatttgga atttttgaag gttgttgttt ttgagatttt gtataaaaag catcatattc
     9061 tttctcttgt ttttgaattt tcctgtcaat attttttatt tcgtttgtag aaggagcatc
     9121 tgtaaatatt ttttgtgcaa ttcttttaaa aacgggtcca gcaacatcgg caccataata
     9181 attgttgttt attgtgcttg gtttatgaac caccactata caagaatatt ttgggctttc
     9241 tgctggaaaa tagcctacaa atgatgaagc ataatatttt tcagaacctc catttttggc
     9301 ataatttact tgtgctgttc ctgtttttcc tgccatcgaa aagtctttgg aataaagtcc
     9361 ttttccagtt ccttttttta caacattttg taaaacggct tttaatttca agatggtttc
     9421 tttagaacaa attttaggat tcatcaccac tttgtcatat tttttgatgg atgtatccca
     9481 ttgtttaatt tcggaaacaa tttgaggctt aaccatttcg ccgttattgg caacagcatt
     9541 atagaaagtg agtgtttgca aaggggttac agaaactcca tagccaaaag ccatccaagg
     9601 aagtgaaagg ttagaccagt ttttgtcact aggttgtggt ataaatggtt tgccttcgcc
     9661 tttgaattct aatcctaatt ttttattcaa tccaaaggag ttaatgtgat ttacaaattg
     9721 tgatggattg tttttgtaat tattataaac cgcttgaacc attacggtat tcgatgacac
     9781 ttcaaatccg cgagctaatg aaattttgcc atatccaccc tcatgagaat ctcttaccga
     9841 ttttccagaa tatttgataa taccaccgta agtattgaaa acggcacttg tgtcaatttt
     9901 tttatcttct aaaagaatca ttagatccac aagtttgaat gtagaaccag gttcgtgtga
     9961 ttcggtgtag gcataattcg tggtttcaaa ataggatcca tcatcatgtc ttcctagatt
    10021 tgaaatagct ttaattttcc cagttttagt ttccataaca actacacaac catggtctgc
    10081 ttggtacaat tctaattgct ttagcaaggc atgatgggca atgtcttgaa tataaacgtc
    10141 tattgttgat attacatcat agccatcttg tgggtctatt tggttcgcat cacttatagg
    10201 tttccattgc ccttttgcaa ttttttgttt taaaaccttt ccgtcttttc cgtttaagta
    10261 ttttcggtat gcccattcaa ttccttttcc atcgggcatt ccgttgggat tttttctttc
    10321 gtaaccaata gttctttctg ctatttcacc aataggatgt tctcttacgg tttcttgttc
    10381 tacaataatt ccacctttga aaggtcccaa tttaaataga ggaaaacctt taattttcat
    10441 aaattcggta taactcaagt cgcgagctat taaataatat ctgtttttgt tggctctggc
    10501 ttttcgtaat tcgtttaaat agaaatagct aggtttactc aataggagag ataatgaatc
    10561 tgccaatgat tttacatttt tatcaaaagc ttctgccttt ggagcaacag catcaaatcg
    10621 tatttcgtaa tttggaattg aagttgccaa aaggcttccg tctgcagaat agatgtttcc
    10681 tttattggct ggaagtacaa aatttttaac cgttctgtct ttggctaatt ttctgtaata
    10741 atctccttca acccattgga tattagtcaa ttttacggca atagctagtg ccaacataaa
    10801 aatgatggtt gctattatat aaattctgta ggatattttt ttatcttcta ctgccatatt
    10861 ttttgaaaga aatttttatc ttctattact tttttaacct ttattttggt aggcggaact
    10921 gtcgatggaa aaatttgttt ttcggctact ttagctgaaa ctgtcgattc catttttagt
    10981 ttcattaatt ctgaacggcg atctacaaat tccgaacgaa gttcttttac cttgttgttt
    11041 agttcagcaa tcttgaatat tttttgttca aatcgttgcg tgttggcaat cataataatg
    11101 gcaagaacaa tcaaaaagat gatgaaacgc cagtttttca tggagtcatc ttctataaga
    11161 aactttgcct tcaatatgct gtaaaccccg tttttcattg aaaatttgaa ccctgaaagg
    11221 gaatttgttt ttgttattta attttttctt gaatcttaaa ttgactttta tattttttcg
    11281 gcaattctta atttggcact tcttgctcta ttattggttt tgatttcggc atcatctgga
    11341 acaatcaatt ttccaatagt tttgaatgga accgaaaaat ttccaaaaaa atcacgctca
    11401 ggttctcctt caaacattcc gtttttcata aatcttttta ccaatcggtc ttctaatgag
    11461 tggtaagaaa tcacgcttag tcttccgcca gggtttaata tttctaggga ttgttctaga
    11521 aattctttta aaacatccat ttcttgattg acttcaattc gaatggcttg atagatttgt
    11581 gccagtattt tatttcgaac tctttcgggt aaatattttg ctaaaacgtc ttttagttcg
    11641 tctgtagttt taattgggtg atgttctcta gcttctacaa ttgttcttgc caaagcagga
    11701 gcatttttta attctccata atccaaaaac actcttttca aattaatgtc gtcatattcg
    11761 ttgacaattc tataggcatt caggtcgttt ttttgactca ttctcatgtc cagttcggca
    11821 tcaaaacgag tagaaaaacc tctttctgca acatcaaatt gatgagatga aacacctaaa
    11881 tcggccaaaa ttccgtccac actttttaca ccataaaagc gcaaaaacct ttttataaac
    11941 ctgaaattct cattaataag cgtaaatctt tcgtctgcca aagcat
//
LOCUS       DAOSNN010000047        11966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5296_length_11966_cov_4.819243, whole genome shotgun sequence.
ACCESSION   DAOSNN010000047 DAOSNN010000000
VERSION     DAOSNN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5296_length_11966_cov_4.819243"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLQ70_03945"
     CDS             <1..867
                     /locus_tag="PLQ70_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQE33800.1"
                     /translation="ILILLFLIARFIAGRSIKPIKIITQTSSQITKDNLKERIVLPQN
                     KDELYVLSKTINDLLDRIENAVEREKQFTSDASHELRTPLTVLKGTLEVLIRKPRTQS
                     EYEEKISYGISEVNRLNNLVDQLLLLARFENQIQSLKTEKVYLNALLLDILTRYSLKA
                     NEKKISINHQFFEEYYVQSDSYLVSIILSNIISNAIKYSNENGAISITLSQNETTTIC
                     TISDNGIGISKEDLGKIANPFYRSNPTNHPEIKGSGLGLSIVKRLAELLNIQFKIQSE
                     INKGTSVILSFK"
     gene            994..2934
                     /locus_tag="PLQ70_03950"
     CDS             994..2934
                     /locus_tag="PLQ70_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HQE33801.1"
                     /translation="MKKIDFLKPIFNFLFIGLFITTLSRIFLFFLFQERVSQTPNYWH
                     IFPIGLRMDLILLCYILFLPTLLICLFPDALLKKIRVFFTIYFVLFLSLILFMELATP
                     NFIGQYDTRPNQLFIEYLIYPKEVIQLLYKGYLLSIIATFIIITAFIYVLIKRRKKLF
                     GIVPSAYKTKLILFPLIAFLLFFGARSSLTSKRPINSSNAIFSTDQLTNCMGLNSFYT
                     VAFALYSIKNEGNSDKVYGKMNPDEAIARVKKYMNVSDNDFIDKEYPLLHNQYSDTLV
                     AKPYNVVIILEESLGAEYVGCLGGLPLTPEFDKLSKEGLLFTNLYCTGTRSVRGIEAV
                     VTGFLPSPSESVVKLSNSQSDFYTIASLLKSKGYDTSFIYGGMDNFDNMGSFFNGNGF
                     DNLIDEEHFDPKKSVFHATWGWSDEDVMKKANDYFRSLKTKPFVSLIFSSSNHEPFEY
                     PEGRIKLYDKQKNTVNNAMKYADYSIGKFFEMAKKEGYYKNTIFIVIADHNTRTYGKH
                     LVPIHKFHIPALIIGPGFSKGEKYSKLCSQIDMQPTLLSKIGMNVITPMPGRNLFLLP
                     ENTKGRAIMQFNDINAFRVDNQVIIMQPNKDPLQFRVEKDTIFTPVTLNKELAKDALA
                     HVITASYLYKERKYNFKENKKK"
     gene            3087..7427
                     /locus_tag="PLQ70_03955"
     CDS             3087..7427
                     /locus_tag="PLQ70_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HQE33802.1"
                     /translation="MLEKIIAFSLRNKLIILLFTLLVIGYGLFSITQISIGAVPDVTN
                     NQVQVITTSRNLSTQDIEQFITYPVEIEMANLPGVKEIRSISKFGLSVVTIVFDDDLG
                     TYLPRQLIAEKIKSASEKIPQGFGTPEMGPITTGLGEIYQYTLEVKPEFKSHYSVTDL
                     RTIQDWVVKRQLSGIKGVVEINTWGGYLKQYEIAIVPSRLKAMNITTLDVFNALEKNN
                     SIAGGAYIEKTNQSYFIRAEGKVKSIQDIENIVVKNTNGLPVYVKNVAEVRYGNANRF
                     GAITGNSEGEKVLGQVMMLKGGNSKQVITDVKNRVAEIQKRLPKGVYINGFLERSELV
                     GKTTFTVAENLILGCLIVIFVVVLLLGNWRSGLVVASVIPLCLLFAISLMNIFGIDAN
                     LMSLGAIDFGIIIDGAVIIVEFIAFQIAHKSKGLENLSDQDLQIEIDQITYKSASKMM
                     NSAIFGQLIILIVFIPILSLTGIEGKMFKPMGMTFSFALLGAMLFCFTYVPVVSSLFL
                     KPTEENPKSISNRLIQLMQSWYIPVITWALANTKKIMYAAIGLLLGAIALFTTMGGEF
                     IPTLDEGDFVIQPVLKTGTSLSKTIATTTKIEQIILKNFPEVEQVVSRIGAAEVPTDP
                     MSMEESDIIVKLKPKSEWVSASSKDELADKIKTAIAKQIPNMDIEFTQPIEMRFNELI
                     SGTRSDVAVKIFGEDLEVLAEKAKEIKKAIQNIEGASDVIIEKTEGLPQMAVSYDRSK
                     IARYGLNIADLNEIIALGFAGKAVGNVFEGEKRFDMVIRLDKTNRRDIEDLRNLYVSV
                     PNGEQIPLSELASIEYTEGPAKISRDNTNRRIVVGINVRNRDLQSVVLDIQNIVENKI
                     KLPVGYSVSYGGQFENLQSAKARLAIAVPIALFLIFILLYFAFSSVKEALMVYSAIPL
                     SAVGGVLFLWLRDLPFSISAGVGFIALFGIAVLNGIVLIEHFKELKHSGMEDMDELIL
                     KGTTDRLRPVILTAAAAALGFLPMAISSSAGAEVQRPLATVVIGGLITATILTMVVLP
                     ILYKVFDGKEFKKAKFKIHKKKTFLLIGLLFTSFAFSQNVSSNSELDSLIAKALENSK
                     GIKAAQLQVDKTKANINNAYSFDKTNIYYSYDQNNLAQNNEPLKVFGIQQRFAFPTVY
                     GAQKNVFTAEYEKEKASYEIQKNRLTLNVSNAYYHIVYLQHQEKLYRYLDSLYQNFSK
                     ASDRKFELGETNYLEKITAQAKFRKISTTLSQIENDKKAQYEILQSLVQSEEKVVISS
                     HKIQPIEKITNETSKLLYSSYLESVTKNYKNQISLQKQHWLPDLNLDYFQGKNSGLSQ
                     SIYGFQVGVGIPLFYFGNRAKSKVAQLELQSWEQEKQNEEQKIDKYINQKMNELAKFQ
                     EAINYYDQYGKKLSEEIIKVGNRSYQQGEIDFFQYILSLENATAIQVDYLDNVLQFNK
                     TQLDLQYLNF"
     gene            7438..8556
                     /locus_tag="PLQ70_03960"
     CDS             7438..8556
                     /locus_tag="PLQ70_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQE33803.1"
                     /translation="MRRIIYNFTIISLLFSCKDAKTEEPLTKDGGLTTVTTAQFQSSG
                     MEIANPVEQDFDVVINTSGRIDVPPQNRAKVTSFIGGYVKSTTLLVGDKVTKGQALLT
                     LENTEYLDIQKDYLEVAEQIKYLKSEFERQKTLYDEKISSQKNYLKAESDYRKTLGIY
                     QSLRSKLQLLNINPSNVEKGKLTSIVTIYAPITGDIVIMNANVGMPVAPSDVILEIVD
                     TNHLHLELAVFEKDILKVKERQKITFTVPEATKEVFDAEVHLVGKSIEGNDRTINVHG
                     HLDDKIKQKLLTGMFVEAKIKINSKQGLAIPVDALIIEGNKNFVLLLDNDKNNIYSFK
                     KVAVTVGEKSEKTIEIIPDDQINTFSKILVKGVFDLAN"
     gene            8615..9127
                     /locus_tag="PLQ70_03965"
     CDS             8615..9127
                     /locus_tag="PLQ70_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33804.1"
                     /translation="MMNDAHLHMVVNHFPIIGTILGLGILIASIVFKNNSVKNTAYVL
                     FIVAALFAAFSMATGEGAEEMVEDMPNIGKHIIHEHEEMAEKMALVLYVLGIISLAGL
                     YLNYKNHAKAKLVSFIALTVAFIGVFLAQQTGTTGGEIRHTEIRANSVSADVKQKSVS
                     GEEGESEDKE"
     gene            complement(9224..11014)
                     /locus_tag="PLQ70_03970"
     CDS             complement(9224..11014)
                     /locus_tag="PLQ70_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQE33805.1"
                     /translation="MNDKTRGGQFIVKETKCEDVFTPEDFNEEQIMMRDSVKEFVDKE
                     IWPNKNRFESKDYAFTEETMKKAGEMGFLSVAVPESYGGMGMGFVNTVLVCDYISGAT
                     GSFSTAFGAHTGIGTMPITLYGTEEQKQKYVPKLASGEWFGAYCLTEPGAGSDANSGK
                     TKAVLSEDGTYYKITGQKMWISNAGFCSLFIVFARIENDKNITGFILENTTDNGISFG
                     EEEHKLGIRASSTRQVFFNETKIPVENMLSERGNGFKIAMNALNVGRIKLAAACLDAQ
                     RRVTSGAVKYANERIQFNTPIAQFGAIRLKLAEMATSCYAGESATYRAAKDIEDRIAV
                     REAEGTSHQESELKGVEEFAIECSILKVAVSEDVQNCADEGIQIFGGMGFSEDTPMES
                     AWRDARIARIYEGTNEINRMLSVGMLIKKAFKGHVDLLGPATKVQEELMGIPSFDAPD
                     YSELFAEEKEMLAKLKKAFLMVAGGAVQKYGPDLEGHQQLLMAAADILIEIYMAESTI
                     LRTEKLAKKEGETNVQEQIAMAKLYLYKAVDVITQKGKESIISFAEGDEQRMMLMGLR
                     RFTKYTNMPNIIGLRETITTKLVTENEYCF"
     gene            complement(11056..11421)
                     /locus_tag="PLQ70_03975"
     CDS             complement(11056..11421)
                     /locus_tag="PLQ70_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQE33806.1"
                     /translation="MRHNFKNLKIWILAMDIANDIYKLTSTFPKSEVYSLSSQMNRCS
                     VSMPSNIAEGSNRGNKHFQYYLNISLGSSFELQTQLLIAFQNNYLSEEKTTKIESKII
                     EFQKMTTGFINKLDNNLSS"
     gene            complement(11468..>11966)
                     /locus_tag="PLQ70_03980"
     CDS             complement(11468..>11966)
                     /locus_tag="PLQ70_03980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HQE33807.1"
                     /translation="LSKLKPVFAADGSVTAGTSSQMSDGAAFVLIMNEEMVKELNLNP
                     IARLVSYASAGVEPRIMGIGPVKAIPKALKQAGLELKDIDLVELNEAFASQSLAVIRE
                     LNINPDIVNVNGGAIALGHPLGCTGAKLSVQLFDEMKRRNNKYGIVSMCVGTGQGSAG
                     IYELL"
BASE COUNT         3997 a         1955 c         2099 g         3915 t
ORIGIN      
        1 attttaattc tattgttctt aattgcgaga ttcattgcgg gtagaagcat aaaacccatc
       61 aaaatcatta cccaaacttc aagccaaatt accaaagata atttgaaaga acgcatcgtt
      121 ttgcctcaaa acaaagacga attgtatgtt ctttcgaaaa ctattaatga tttattggat
      181 cgcatcgaaa atgctgtaga acgtgaaaaa caatttactt cggatgcttc acacgaatta
      241 agaactccac tgaccgtttt gaaaggaacc ttagaagtct tgattaggaa acccagaact
      301 cagtcagaat atgaagagaa aattagttat ggaatttcgg aagtcaatag attaaataat
      361 ttggttgatc aattgctgtt attggcaaga ttcgaaaatc agattcaaag tttgaaaacc
      421 gaaaaagtat atttgaatgc cttattatta gacattttaa caagatattc attgaaggct
      481 aatgaaaaaa agattagtat aaatcatcaa ttttttgaag aatattatgt tcaatcagat
      541 agttatttag tgtcaattat tttaagtaat atcattagca atgccatcaa atattcgaat
      601 gaaaatgggg cgatttcaat aacgctttcg caaaatgaaa cgactacgat ttgcaccatt
      661 tcagacaatg gaatcggtat ttcgaaagaa gatttaggta aaattgcaaa tcctttttat
      721 cgttcgaacc caaccaatca tcctgaaata aaaggttcag gcctaggact ttccatcgta
      781 aagcgattgg cagaattatt aaacattcaa tttaaaattc aaagcgaaat caataaagga
      841 acttcggtaa ttttaagttt taaataatat aaaataagca ttatctaagt gttagtagaa
      901 tattaattaa atattaaggt tgtttgtatt tttgatttga tttaaatacc tatttttata
      961 aattattagt aaactccagt tttatattat ataatgaaaa aaatagactt ccttaaaccc
     1021 atatttaact tcttgtttat tgggttattc attaccactt tatcccgaat ttttttattc
     1081 tttctatttc aagaaagagt atcacaaact ccaaattatt ggcatatttt tcctattggt
     1141 ttgcgaatgg atttgatttt attgtgttac attctttttc ttccaacgct attaatttgc
     1201 ctatttccgg atgctttatt gaaaaaaata agggtgtttt ttactattta ttttgtttta
     1261 ttcttgtcct tgattttgtt tatggagttg gcaactccca attttattgg acaatatgat
     1321 accagaccta atcaattatt tattgaatat ctaatttatc ccaaagaagt tattcagttg
     1381 ctttacaaag gatatctact atctattatt gctactttta taatcattac ggcatttatt
     1441 tatgtcttga taaaacgtag aaaaaaactt tttgggatag ttccatctgc atacaaaacc
     1501 aagttgattt tatttccttt gattgctttt ttattgtttt ttggtgctcg ttctagtcta
     1561 acttcaaaaa gacccattaa ttccagtaat gccatatttt cgaccgatca attgacgaat
     1621 tgcatgggat tgaattcctt ttatacggtt gcttttgctt tgtattcaat taaaaacgaa
     1681 ggtaatagtg ataaagttta tgggaaaatg aatcctgatg aagctattgc tagggtcaaa
     1741 aaatatatga atgtttctga taatgatttt attgataaag aatatccttt gctacacaat
     1801 caatattcgg atactttggt tgcaaaaccg tataatgttg ttattattct cgaagagagt
     1861 ttaggtgcag aatatgttgg ctgtttgggt ggattgcctt taactcctga atttgataaa
     1921 ttgtcaaaag aaggtttatt gtttactaat ttatattgta caggaacccg aagtgtacgt
     1981 ggaattgaag ctgttgttac aggtttttta ccttcgccat cagaaagtgt tgtgaaatta
     2041 agtaattcac aaagcgattt ttatactatt gccagcttgt taaagtcaaa aggttatgat
     2101 actagtttta tttatggagg tatggataat tttgacaata tggggtcatt ttttaacgga
     2161 aacggattcg ataatttaat tgatgaagaa cattttgatc caaaaaaatc agtttttcat
     2221 gctacttggg gatggtctga tgaagatgta atgaaaaagg ctaatgatta ttttaggtca
     2281 ttaaaaacca aaccttttgt ttcattaatt ttttcttcct ctaatcacga accttttgaa
     2341 tatccagaag gaagaattaa attgtatgac aaacaaaaaa atacggttaa caacgcaatg
     2401 aaatatgccg attattctat tggaaagttt tttgaaatgg ccaaaaaaga agggtattat
     2461 aaaaacacta ttttcattgt aattgctgac cacaatacta gaacgtatgg aaagcatttg
     2521 gttccgattc acaaatttca tattccagca ttaatcattg gaccaggttt ttcaaaaggg
     2581 gaaaaataca gcaaattatg cagtcaaatt gatatgcaac ccacactttt aagcaagatt
     2641 ggaatgaatg ttattacacc aatgcctgga agaaatttgt ttctccttcc tgaaaatact
     2701 aaaggacggg caataatgca attcaatgac atcaatgctt ttagagtaga taaccaagta
     2761 attattatgc aacccaacaa agatcctttg caatttaggg ttgaaaaaga cactattttt
     2821 actccagtaa ctttgaataa agaattggca aaagatgctt tggcacacgt gattaccgct
     2881 tcttatttgt ataaagaaag aaaatacaat ttcaaagaaa ataagaaaaa ataggcagat
     2941 tgctgtatct ttataatgta ttcgaaatca aaaaaccgtt gaatgaaact acaatatttt
     3001 aagtttttcg taagcatatc ttaagttgtt tttaagcatt ttgtcttcta tttctcaata
     3061 cttttgtgac ataaaattag aattatatgt tggagaaaat tatcgcattt agcttacgaa
     3121 ataaactaat cattttacta tttacacttt tggttattgg atatgggctt ttttccatta
     3181 cccaaatttc cattggcgca gttccagatg tgaccaacaa tcaagtgcaa gtaatcacga
     3241 cttctcgcaa tctttcgacc caagatatag agcaatttat tacctatcct gtcgaaattg
     3301 aaatggctaa tttgcctggt gtcaaagaaa tccgttccat atcaaaattt gggttatcgg
     3361 tagtaaccat cgtttttgat gatgatttgg ggacctatct tccacgccaa cttattgccg
     3421 aaaaaataaa atcggcttcc gagaaaatac ctcaaggttt tggaaccccc gaaatgggtc
     3481 ctattactac tggtttgggc gaaatatatc aatatacgct ggaagtaaaa ccagaattca
     3541 aatctcacta ttccgttact gatttgagga ccattcaaga ctgggtagtg aaaaggcaat
     3601 tatctggaat caaaggtgtt gtagaaatca atacttgggg tggttatctc aaacaatatg
     3661 aaattgccat cgttccctca cgtttaaaag caatgaatat cacaactttg gatgtattta
     3721 atgctttaga aaaaaacaac agtattgcag gtggtgctta tatcgaaaaa acgaatcaaa
     3781 gttatttcat tcgtgccgaa ggaaaggtaa aatccattca agacatcgaa aatatcgtgg
     3841 tcaaaaacac aaatgggttg ccagtttatg tcaaaaatgt ggccgaagtt cgttatggaa
     3901 atgccaatcg ttttggtgcc ataacgggta atagcgaagg cgaaaaagtt ttaggtcaag
     3961 tgatgatgct caaaggagga aactcgaaac aagtaatcac agatgtcaag aatagggttg
     4021 ccgaaattca aaaacgttta ccaaaaggcg tttacattaa cggttttttg gaacgaagcg
     4081 aattggttgg aaaaactact tttaccgtag ccgagaactt gattttaggt tgtttgattg
     4141 ttatttttgt agtggtttta ttgttaggca attggcgatc gggtttggtc gttgcatcgg
     4201 tgattccgtt gtgtctgctt ttcgctattt cattgatgaa tatttttgga attgatgcta
     4261 atttgatgag tttaggcgcc atcgattttg gaattatcat tgacggagcc gtgattatag
     4321 tcgagtttat tgcattccaa attgctcaca aatccaaagg tttagaaaat ttatcggatc
     4381 aagatctcca aatcgagatt gaccaaatca cgtataaaag tgcttccaaa atgatgaatt
     4441 cagccatttt tgggcaatta attatcctga ttgtttttat accaattcta tctttgacgg
     4501 gcatcgaagg taaaatgttt aaaccaatgg gaatgacttt tagttttgcc ttattgggag
     4561 cgatgttgtt ttgttttacc tatgttcccg ttgtttcctc actattttta aaaccaaccg
     4621 aagaaaatcc aaagtcaata tccaatagat taattcaact aatgcaatct tggtatattc
     4681 ctgtaattac ttgggctttg gccaatacga aaaagataat gtatgctgca ataggtttat
     4741 tactaggagc tatagctttg tttacaacga tgggcggaga gtttattcca acattagacg
     4801 aaggtgattt tgtgattcaa cccgttttaa aaacaggaac ttcactatca aaaactattg
     4861 ctacaactac aaaaattgag caaattattc tgaaaaactt tcccgaagtg gagcaagtcg
     4921 tgagcagaat tggtgctgcc gaagtgccaa ccgatccaat gagtatggaa gaaagtgata
     4981 ttattgtcaa attaaaaccc aaatcagaat gggtttccgc ttctagcaaa gacgagttgg
     5041 ccgataaaat aaaaacggct attgccaaac aaattccaaa tatggatatc gaatttacgc
     5101 aacctattga aatgcgattc aacgaattga tttcaggaac acgttctgat gtggcagtga
     5161 agatttttgg cgaggattta gaagtattgg ctgaaaaagc aaaagaaatt aaaaaagcca
     5221 ttcaaaatat cgaaggagca tcggatgtaa ttatcgaaaa gaccgaaggt ttacctcaaa
     5281 tggcagttag ttatgaccgt tctaaaattg ctcgttacgg tttgaatatc gccgatttga
     5341 acgaaataat tgcacttggt tttgctggaa aagcagttgg aaatgtgttc gaaggcgaaa
     5401 aacgtttcga tatggtcatt cgtttggaca aaaccaatcg tcgagatatt gaagatttac
     5461 gaaatttata tgtttcggtt ccaaatgggg aacaaattcc cttgagcgaa ttggcaagta
     5521 tcgaatatac tgaaggtcca gcaaaaatat ctagagacaa taccaatcga agaattgtgg
     5581 tgggaattaa cgtgagaaat cgagatttac aaagtgttgt tctcgatatt cagaatattg
     5641 tggaaaataa aattaaactc cctgttgggt attctgtcag ttatggaggt caatttgaaa
     5701 accttcaaag tgccaaagct cgtttggcta tcgccgttcc aatagctttg ttcttgattt
     5761 ttattttgtt gtattttgcc tttagcagcg tcaaagaagc cttgatggtg tattcagcaa
     5821 ttccgctttc ggcggtaggt ggtgtgttgt ttttatggtt gcgagattta ccgtttagta
     5881 tctcggcagg tgttggtttt attgcacttt tcggaattgc agtgctcaac ggaattgtac
     5941 ttattgaaca ttttaaagaa ttaaaacaca gcggaatgga agatatggac gaattaattt
     6001 taaaaggaac aaccgataga ttgcgacccg taattttgac agcggcagca gcagcattgg
     6061 gatttctgcc aatggcgatt tcatcatcgg ctggtgcaga agtgcaacgt ccgctggcta
     6121 cggtggttat aggaggtttg attacagcaa caattttaac gatggtggtt ttacctattt
     6181 tgtacaaagt ttttgatgga aaagaattca agaaagcaaa atttaaaatt cataaaaaga
     6241 aaacctttct tcttatcgga ttgttgttta cttcatttgc tttttctcaa aatgtaagtt
     6301 caaatagtga actcgactct ttgattgcca aggcattgga aaatagtaaa ggaataaaag
     6361 cggctcaatt gcaagtcgat aaaacaaaag cgaacatcaa taatgcctat tcgtttgata
     6421 aaaccaatat ttattacagt tatgaccaaa ataatttggc tcaaaataac gaacctttga
     6481 aggtttttgg aatccaacaa cgttttgctt ttccaacggt ttatggagcg caaaaaaatg
     6541 tctttactgc cgagtacgag aaagaaaagg ctagttatga aatccagaaa aacagattga
     6601 ctcttaatgt ttccaatgca tattatcata ttgtgtattt gcaacatcaa gaaaaattgt
     6661 atcgttattt ggatagtttg taccaaaatt tctccaaagc tagcgatcgt aaatttgaat
     6721 tgggagaaac caattatctt gaaaaaatta cggctcaagc caagtttaga aaaattagca
     6781 ccacgctttc acaaattgaa aatgacaaaa aagcgcagta tgaaatctta caatcactag
     6841 tacaatcaga agaaaaagta gtcatttcaa gtcataaaat tcaaccaatt gaaaagataa
     6901 ctaatgaaac aagtaagttg ttgtattctt cttatttaga aagtgttact aaaaattata
     6961 aaaaccaaat cagtttgcaa aaacagcatt ggttacccga tttaaaccta gattattttc
     7021 aaggaaaaaa tagtggttta tcccaatcta tatatggctt tcaggttgga gttggaattc
     7081 cactgtttta ttttggtaac agagcaaaat cgaaagttgc ccaattggaa ttgcaatctt
     7141 gggagcaaga aaagcaaaat gaagagcaaa aaatagacaa atacattaat caaaaaatga
     7201 atgaattggc caagttccaa gaagcgatta attattacga tcaatatgga aaaaaactct
     7261 cggaagaaat tatcaaagtg ggaaatagaa gttaccaaca aggtgaaata gatttctttc
     7321 aatacatttt aagcttagaa aacgcgacag ccattcaggt ggattattta gacaatgtgc
     7381 tgcaattcaa caaaacccaa ctggatttac aataccttaa tttttaatat gttacctatg
     7441 agaagaatca tatataactt cacaataata tcacttttat tttcttgtaa agacgctaaa
     7501 acagaagaac cattaacaaa agatggtgga ctaaccactg ttacaaccgc tcaatttcaa
     7561 tcttcgggta tggaaattgc aaatcccgtt gaacaggatt ttgatgttgt tatcaacact
     7621 tcaggaagaa ttgatgtgcc acctcaaaat agggcaaaag tgacttcgtt tattggtgga
     7681 tatgtcaaat ccactacgct tttggttgga gataaagtga ctaaaggtca ggcactttta
     7741 acgttagaaa atactgaata tcttgatatt caaaaagatt atctagaagt agccgagcaa
     7801 attaaatatt taaaatcgga attcgaaaga caaaaaacac tttatgacga aaaaataagt
     7861 tcccaaaaaa attacctcaa agcggaaagt gattatagaa aaactttggg aatatatcaa
     7921 agtttaagat ctaaactgca attgttgaac atcaatccca gcaatgttga gaaaggaaaa
     7981 ttaacatcaa ttgttactat ttatgcccca attacgggag atattgtcat tatgaatgcc
     8041 aatgttggaa tgccagttgc tccttcggat gtaattttgg aaattgtaga taccaatcat
     8101 ttacatttgg aattagccgt atttgaaaag gacattttaa aggtaaaaga gaggcaaaag
     8161 attactttta ccgttcctga agctaccaaa gaagtttttg atgcagaagt acatttggtt
     8221 ggaaaatcaa ttgaaggaaa tgatagaacc atcaatgttc acggacattt agatgataaa
     8281 ataaaacaaa aattacttac aggaatgttt gttgaagcca agataaaaat caattcaaaa
     8341 caaggattgg cgattcctgt agatgcatta attattgagg gcaataaaaa cttcgtactt
     8401 ttattggata atgacaaaaa caatatttat tcctttaaaa aagtggctgt aactgttggt
     8461 gaaaaatctg aaaaaacaat agaaattatt cctgacgatc aaattaatac tttttcaaaa
     8521 atactagtaa aaggcgtttt tgatttggca aactaaaaat tattcttttg aattaaagcg
     8581 ttataaacag taaaaaaata actttaaata aaaaatgatg aacgacgcac atttacacat
     8641 ggtagtaaac cattttccaa ttatcgggac gattttggga ttaggaattt taatagcaag
     8701 tattgttttt aaaaacaatt cggtcaaaaa cacggcttat gttttgttta ttgtagcagc
     8761 actttttgcc gcatttagta tggcaacagg tgaaggagca gaagaaatgg tagaagatat
     8821 gccaaatatt ggaaaacata ttattcacga acacgaagaa atggcagaaa aaatggcact
     8881 tgtattgtat gttttgggaa taatttcact cgctggatta tacttgaact ataaaaatca
     8941 tgctaaggca aaattggttt ccttcatagc tttgaccgtt gcttttattg gagttttttt
     9001 agcgcaacaa acaggaacaa ctggtggaga aattcgtcac actgaaataa gagccaattc
     9061 agtttcagca gatgttaaac aaaaatcggt tagtggagaa gaaggagaaa gcgaagacaa
     9121 ggagtaagaa attatttttg tcaagcaata aattatatgc aaaaagagag gataatatta
     9181 actatcctct cttttttatt tgaattagtt agtttttttg atattagaaa caatattcgt
     9241 tttcagttac taatttggtt gtaatggttt ctcttaagcc aatgatatta ggcatattgg
     9301 tgtattttgt aaaacgtctt aatcccatca gcatcattcg ttgttcatcg ccttcggcaa
     9361 aagaaataat gctttctttt cctttttgag ttattacgtc aacagcttta tataaataca
     9421 atttagccat agctatttgc tcttggacat tagtttcacc ttctttttta gctaattttt
     9481 cggttctcaa aatggtcgat tctgccatat aaatttcgat caaaatatcg gcagcagcca
     9541 tcaataattg ttggtgtccc tctaaatcgg gtccgtattt ttgaacggct ccaccagcaa
     9601 ccattaggaa cgcttttttc aatttagcca acatttcttt ttcttcggca aataattccg
     9661 aataatcagg agcgtcaaaa gacggaattc ccattaattc ttcttgaact ttagtagctg
     9721 gccctaataa atcaacgtga cctttgaatg cctttttaat caacattcct actgaaagca
     9781 ttcggttaat ctcgtttgta ccttcataaa ttcgggcaat tctggcgtca cgccaagcac
     9841 tttccattgg ggtatcttca gaaaatccca ttccaccaaa aatttgaatt ccttcgtcag
     9901 cacaattctg aacatcttca gaaaccgcta cttttagaat tgaacattcg atagcaaatt
     9961 cttcaacacc tttcaattcc gattcttgat gcgaagtgcc ttcagcttct ctaacggcaa
    10021 ttcgatcttc aatatctttg gcagctctgt aagtggcact ttcacccgca taacaagaag
    10081 ttgccatttc ggctaatttc aaccgaatcg ctccaaattg agcaatgggc gtgttgaatt
    10141 gaattctctc gttggcatat ttcacagcac ccgaagttac tcttcgttga gcgtcaagac
    10201 aagcagctgc taatttaata cgacctacat tcaacgcgtt cattgcaatc ttgaacccat
    10261 ttcctctttc agaaagcata ttttcaacag ggattttggt ttcattaaag aaaacttgac
    10321 gagtcgaaga ggcacgaatt cctaatttgt gttcttcttc tccaaatgaa attccgttat
    10381 ccgtggtgtt ttctagaata aatcctgtaa tatttttatc attttcaatt cgtgcaaaaa
    10441 cgataaataa agaacaaaat ccagcattgg aaatccacat tttttgcccc gtaattttat
    10501 aatatgttcc atcttcggac aaaacagctt tggtttttcc agaattggca tcgcttccag
    10561 ctccaggttc agtcaagcaa taggctccaa accattctcc agaagccagt ttaggaacgt
    10621 atttttgctt ttgttcttcg gttccataca aagtaatggg cattgttcca attccggtat
    10681 gagctccaaa agcagttgaa aacgaacctg tagcacctga aatatagtca cataccaaaa
    10741 cggtatttac aaatcccatt cccattccgc cataagattc aggtactgcc acactcagaa
    10801 agcccatttc tccagctttt ttcatagttt cttcagtaaa agcataatct ttgctttcga
    10861 aacgattttt gttaggccaa atttctttgt ctacaaattc ttttaccgaa tcacgcatca
    10921 taatttgctc ttcattgaaa tcttctggag tgaagacatc ttcgcatttt gtttctttta
    10981 cgatgaattg accaccgcga gttttatcgt tcatattttt agatttaatg gttgtttaga
    11041 taaaaggtga aagaatcaag aagaaagatt gttgtctaac ttatttatga aacctgttgt
    11101 catcttttga aattcaatta ttttgctttc tattttggtt gttttttctt ctgatagata
    11161 attattttga aaagcaatca acaattgagt ttgcaattca aatgatgagc ctaaactaat
    11221 attcaaataa tattgaaaat gcttatttcc tctatttgaa ccttctgcaa tattggaagg
    11281 cattgaaacc gaacaacggt tcatttgact tgacagacta tatacttcag attttggaaa
    11341 agttgaagtt agtttgtaaa tatcattggc aatatccata gctaaaatcc aaattttcaa
    11401 gttcttaaaa ttatgcctca tttaaactct tgtttcttta ttcttgtttc tattttcttg
    11461 actcttttta caacaattca taaatccccg cactgccttg tccagttcct acgcacatgc
    11521 tcactattcc gtatttatta tttctgcgtt tcatttcgtc gaatagttga acagataatt
    11581 ttgcacccgt acagcccaaa ggatgaccta aggcaattgc accaccatta acgttcacaa
    11641 tatcaggatt gatgttcaac tcccgaatta ctgctaatga ttgcgaggcg aatgcttcgt
    11701 tcaattccac caaatcaatg tctttcaatt ctaatccagc ttgttttaag gctttcggaa
    11761 tggcttttac tggaccaatt cccataattc tgggttcaac tcctgcggac gcatagctaa
    11821 ccaaacgggc aataggattg aggttcaatt ctttgaccat ttcttcgttc ataatcaaaa
    11881 caaaagcggc tccatcgctc atttgggatg aagttcctgc tgttacactt ccgtcagccg
    11941 caaaaacagg ttttagtttt gataat
//
LOCUS       DAOSNN010000048        11934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5316_length_11934_cov_5.728681, whole genome shotgun sequence.
ACCESSION   DAOSNN010000048 DAOSNN010000000
VERSION     DAOSNN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11934
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5316_length_11934_cov_5.728681"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..515)
                     /gene="mraZ"
                     /locus_tag="PLQ70_03985"
     CDS             complement(45..515)
                     /gene="mraZ"
                     /locus_tag="PLQ70_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQE33808.1"
                     /translation="MNTIVGTYECKVDAKGRILMPAPLKKQLATSLQNGFVLKRSVFQ
                     PCLELYPMEEWDLMMQKINKLNRFVKKNNDFIRRFTAGVKMVEIDALGRLLVPKDLVV
                     FANISKDVVFSSAVNIVEIWDKELYEKSISGEDVDFADLAEEVMGNTNDDHGIS"
     gene            866..1639
                     /locus_tag="PLQ70_03990"
     CDS             866..1639
                     /locus_tag="PLQ70_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQE33809.1"
                     /translation="MEKYFKKEGRYSYYEAGEGTPIVVLHGLMGGLSNFDAVASYFSE
                     KGYKIVIPDLPIYTQSILKTNVKAFARYVKDFITFKGFDRVILLGNSLGGHIALYHTK
                     MYPEKVAGLVLTGSSGLYESAMGDSYPKRGDYEYIKKKAEAVFYDPAMATKELVDEVY
                     ASVNDRIKLIKTLTIAKSAIRHNMAKDLPKMHVQTCLIWGKNDSVTPPNVAEEFNELL
                     PNATLYWIDKCGHAAMMEHPDEFNRLLEDWLTHTHLKAH"
     gene            1658..2278
                     /gene="yihA"
                     /locus_tag="PLQ70_03995"
     CDS             1658..2278
                     /gene="yihA"
                     /locus_tag="PLQ70_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQE33810.1"
                     /translation="MKINTAEFIVSNSEVDKCPKDFLPEYAFIGRSNVGKSSLINMLT
                     NHKNLAKTSGRPGKTQLINHFKINNNWFLVDLPGYGYAKVSKKTKSVFQQFITDYFEK
                     REQLVCAFVLIDIRHEAQNIDIEFMEYMGESEIPFCIIFTKADKISKVKIDSHIAAYK
                     KKMFANNWAEMPQYFVTSATEMTGKDEVLTFIDEVNQEVFKNNSEF"
     gene            complement(2351..2689)
                     /gene="gldC"
                     /locus_tag="PLQ70_04000"
     CDS             complement(2351..2689)
                     /gene="gldC"
                     /locus_tag="PLQ70_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HQE33811.1"
                     /translation="MANKNTSEIKINVELDENRVPEKLSWAAVEGGVELESAKAFMLS
                     VWNSDERATKCIELWTKDMSVDEMKIFFHQTLLSMTETFQRATGDEKMSATMQDFCDY
                     FAEKLELKAK"
     gene            complement(2679..3644)
                     /gene="gldB"
                     /locus_tag="PLQ70_04005"
     CDS             complement(2679..3644)
                     /gene="gldB"
                     /locus_tag="PLQ70_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldB"
                     /protein_id="HQE33812.1"
                     /translation="MKKLIIVIALTFAFVCCDKKTKIEKTVAEIPVEIKVERFDKLFF
                     ETEPKNLGQLKKQYPFFFPPGNDDSVWLEKMQNPLWRELYTEVQKKYSDFGPVTKELN
                     SLFKHIKYYFPENKTPKVITVISEMDYNNKVIYTDSLVIISLELYLGKDHKFYQFPNY
                     LKQNFEPKQILPDVVSSFSIQKIAPITNKNLLSQMVYFGKQLYLKDILLPEYSDADKI
                     GYTLEQNKWCVENEGYMWRYFLENQMLYSDDQKLNNRFINPAPFSKFYLEIDNESPGR
                     VGTWIGWQMVRSFMKNNDVPLEKMLQMNAKELFEKSKYKPKKDGE"
     gene            3788..4594
                     /gene="nadE"
                     /locus_tag="PLQ70_04010"
     CDS             3788..4594
                     /gene="nadE"
                     /locus_tag="PLQ70_04010"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQE33813.1"
                     /translation="MSKISTLQVENANAYIVNWLKTYAENAKVNGFVIGISGGVDSAV
                     TSTLCAQTGLTTLCVEMPIHQAPSQVSRGREHIEQLKKRFSNVSNVEADLTAVFDTFK
                     TIVPQVDNLDIVNLSLANTRARIRMTTLYYFAGIHGLLVAGTGNKVEDFGVGFYTKYG
                     DGGVDLSPIADLMKSDVYALGSYLKIPNSILQASPTDGLFGDDRTDEDQLGASYDELE
                     WAMLEDDKGNVSDNFSGREKEVFDIYKKLNTINQHKMTEIPVCCIPKDFK"
     gene            4681..5310
                     /locus_tag="PLQ70_04015"
     CDS             4681..5310
                     /locus_tag="PLQ70_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQE33814.1"
                     /translation="MIKVCLANNHPVVHFGVKSYFKDHDDISIVANVGNFLMVRDILL
                     TKEIDVLILDLELEGLASIFEVKNILKNFPKTKIIIFSDLSEQIYAPNAIKAGVSGYV
                     SQKEKLETLGQAIVKVNQGKIIIDETVKKNLALIAKQNKSERLYRKLSNREVEVLRYL
                     SDGKKNNEISKILGLNEKTISTYKLRLLQKLNVTNLVDLVNKAKTLEIV"
     gene            complement(5307..7286)
                     /gene="dnaG"
                     /locus_tag="PLQ70_04020"
     CDS             complement(5307..7286)
                     /gene="dnaG"
                     /locus_tag="PLQ70_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQE33815.1"
                     /translation="MISKATIDTVFETARVEEVIGDFVQLKRAGSNFKGLSPFSDERS
                     PSFMVSPAKGIWKDFSSGKGGNAIAFLMEHSHFTYPEAIRYLAKKYNIEIEETEQTDE
                     EKANTDVRESMYLVSEFAKDYFHNTLLHSEEGKAIGLSYFKERGFTGETIKKFVLGYS
                     PETWDALTKEALGKGYKLEFLESTGLTIPRDDRPFDRFKGRVMFPIQSMSGRILGFGG
                     RILTNDKKAAKYLNSPESEIYHKSKVLYGIFQAKQAIAKENNCYLVEGYTDVIQFHQA
                     GIENVVASSGTALTPDQIRLINRLTKNITVLFDGDAAGLRASIRGIDLILEEGMNVKV
                     CAFPDGEDPDSFSKKTPYEELVKYLDENSKDFIQFKASMLMNEAKNDPIKKADLIRDM
                     VVSISKIPDRIQREIYIQECSRIMDISEQVLVSTLAQLVQKDVTELGKKQKQEQKAFE
                     VVKNEAPVEVRKVDILYGLERKIIEILLLYGSKTEEFEDVLLKTNEAGDIENVTEKKE
                     YKVFQRIYLSLQEDEVELANPLFRDIYNNLINYFHQNENFSIEQYLMHLHPDFAQEVT
                     DILMADERVVLHNWEGQNIFPKTKNETISQYVSETILSLRWYLVDKIIEEIKNSISSE
                     PDSNNIEPLSMAMDYSKLINSFSKKLGRVMSRYSN"
     gene            complement(7463..8527)
                     /locus_tag="PLQ70_04025"
     CDS             complement(7463..8527)
                     /locus_tag="PLQ70_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33816.1"
                     /translation="MTKIIFYITVFMLLCISKIQAQDSIHKGDQENRTFEKQVKLIAT
                     KIETITKEEKSALKGEVEAVNIQLENGLITKEKAAIEKQKLAENRAKNIEARVAVEQE
                     NLKNLVQEKVDGNITSLDSSGRKTFVIPSMKIINSNNKIEKRSTSQFVLAFGANNLVT
                     DEAVANSDFYYWQSHFFEWGFTYNTRIFRNYNLLHLKYGFSVMYNNICPTDNRYFVPT
                     TNNQTNLEVFPYKLEDSRFKNVYLVAPLHLEFDFSGNKFKNGSTGFSTHKGVRFGIGG
                     YGGFRIKSKQKMYYNIDDDKIRIKTKGDFNANDFIYGVSTYLGYKETSLYLKYDLNPL
                     FKNNPVDQNNISLGIRFDFN"
     gene            complement(8569..9246)
                     /locus_tag="PLQ70_04030"
     CDS             complement(8569..9246)
                     /locus_tag="PLQ70_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33817.1"
                     /translation="MERNKVEKQFKEKLNSREIKPTDMAWDRLNAMLSVAEEKKSKPK
                     YSWVYIAASFIGLSLIATVFLNQKDYPIDTKINTAIIEKSSPKENSKNGIKSTKQESD
                     FPKTILNSETKPLVQTQRSNNPKVENLELKTQGNVLAETHPKNESLLIVEHKNDIQTN
                     IDSLLASVEEKSNSNFKKSSIKINSNTLLNQVDGELQLSFREKALQSITKKYKEAKEA
                     LVNRNNQ"
     gene            complement(9233..9793)
                     /locus_tag="PLQ70_04035"
     CDS             complement(9233..9793)
                     /locus_tag="PLQ70_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQE33818.1"
                     /translation="MKVIHFNQEENEIIRLAIENNRAAQQKIYAKFSPKMLSVCRQYI
                     KDIHQAEDIMITAFMKVFTNLKNFEQKGSFEGWIRRIMVNECISNIRVQKKVSFVEDE
                     NYFEESFNNIESQFSVEDIQFLIDNLPDGYRMIFNLYAIEGYKHQEIASMLGINEGTS
                     KSQLSHARKILKEQILTLKNYSNGTE"
     gene            complement(9933..10910)
                     /locus_tag="PLQ70_04040"
     CDS             complement(9933..10910)
                     /locus_tag="PLQ70_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQE33819.1"
                     /translation="MNITSQIKQPIFNEMELFEKKFYESMTSKVALLNRITYYIVNRK
                     GKQMRPMFVFLTAKMVSAGIVNERTYRGASVIELIHTATLVHDDVVDDSNRRRGFFSI
                     NALWKNKIAVLVGDYLLSKGLLLSIDNGDFDLLRIISVAVREMSEGELLQIEKARRLD
                     ITEDVYYEIIRKKTATLIAACCALGAKSVIEDEIQVENMRKFGELIGMAFQIKDDLFD
                     YTEDAIGKPTGIDIKEQKMTLPLIHVLNNCTPKEKSWVINSIKNYNKDKKRVKEVIAF
                     VKNHNGLFYAEQKMAEFQQEALSLLDNYAQSEFKDALILMVNYVIERKK"
     gene            11253..>11934
                     /locus_tag="PLQ70_04045"
     CDS             11253..>11934
                     /locus_tag="PLQ70_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33820.1"
                     /translation="MKAKILLFVTMLSIVTFVGCNSNETVDGSTTTAAITADETVVNS
                     EIDATVDDVSIIAEDQYDVQKSASSTAKTTAGMVSMLPACATITKVLTNDTWTRTIDF
                     GTQGCALPNGNIVKGKIIISFSKNFTTPIRTISYTLEGFYHNDKLIEGSKTITHELKT
                     SDLLATIHPVTTHSVDVKITYPDGKIYTRIGTRVREMVEGFATITNWEDNVFKVWGYN
                     ITTFPNGSK"
BASE COUNT         3744 a         1931 c         2013 g         4246 t
ORIGIN      
        1 gtttgatatt caaaccatca accgttggat gaagtaaaac aggattatga tattccatgg
       61 tcgtcatttg tgtttcccat tacttcttcg gctaaatctg caaaatctac atcttcgccg
      121 cttattgatt tttcatataa ctctttgtcc caaatctcaa cgatatttac tgcggacgaa
      181 aacacaacgt ctttcgaaat attggcgaaa accaccaagt ctttcggtac caataatctt
      241 cctaaagcat cgatttcgac catttttaca ccagcggtaa aacggcggat aaagtcgttg
      301 tttttcttta cgaaacggtt gagtttgttg attttttgca tcatcaaatc ccattcttcc
      361 ataggatata attctaaaca aggttgaaaa acagaacgct tcaaaacaaa accgttttga
      421 agtgaagtag ccaattgctt tttcaaaggt gcaggcatta aaatccttcc tttggcatcg
      481 actttacatt cgtatgttcc tacaattgtg ttcaattgat ttgtttataa tgatgatgag
      541 caaaaatata aaaataatta ccacaattta ccactatctc ccacattgtt aataagtttt
      601 accacttcaa gccgagaatc cttagttttt agtcaatcaa gatgttagct gtggttttat
      661 ctctgattat gaatgataga tttatgtgta aattttataa gtaaaaactt taaaatagta
      721 tttaattttc aattctgttc tgagttattt tagaagaact gggtgatgag tgggcagaaa
      781 aaaatgataa aatttgattc caagaattct ttttaatttt ttaaaaccta tctttgtcgc
      841 aattgaaaaa tttgcgataa aacgtatgga aaaatacttt aagaaagaag gtcgatacag
      901 ctattatgaa gctggagaag gaaccccaat tgtggtctta cacggtttaa tgggaggttt
      961 gagcaacttt gacgctgttg caagctattt ttctgaaaag ggatacaaaa ttgtaattcc
     1021 agatttgcct atttataccc aaagtatttt gaaaaccaat gtcaaagctt ttgcaagata
     1081 tgtaaaggat tttatcactt tcaaaggatt tgatagagtg attttgttgg gaaattcgct
     1141 aggaggtcat atcgcattat atcacaccaa gatgtatcct gaaaaagtag ctggacttgt
     1201 attgactggt agttcaggac tttatgaaag tgctatgggc gatagttatc caaaaagagg
     1261 ggattatgaa tacatcaaaa agaaagccga agcggttttt tatgatcctg caatggctac
     1321 caaagaattg gtggacgagg tttatgcatc agtaaatgat agaataaaat tgattaaaac
     1381 attgacaatt gccaaaagtg ccattcgaca taacatggca aaagatttgc caaaaatgca
     1441 tgttcaaacc tgtttgattt gggggaaaaa tgacagtgta acaccgccaa atgttgccga
     1501 agaatttaac gaattattgc caaacgctac tttgtattgg atagacaaat gtggacatgc
     1561 tgctatgatg gaacatcctg atgagtttaa tcgtttattg gaagattggt tgacacacac
     1621 ccatttgaaa gcgcattaaa aatagtacct ccaaattatg aaaattaaca cagccgaatt
     1681 tattgtaagc aactctgaag ttgacaaatg tccgaaggat tttttgccag aatatgcttt
     1741 tataggtcga tccaatgttg ggaaatcatc attgattaat atgctgacca atcataaaaa
     1801 tttggccaaa acatctggaa gacctggaaa aactcaattg ataaatcatt tcaaaatcaa
     1861 taacaattgg tttttggtcg atttgccagg ttatggttat gctaaggttt cgaagaaaac
     1921 aaaatctgtt tttcagcaat ttattaccga ttatttcgag aaaagagaac aattagtttg
     1981 tgcttttgtc ttaattgata ttcgtcacga agcccaaaat attgatattg aatttatgga
     2041 atatatgggc gaaagcgaga ttccgttttg cattattttt accaaagccg acaagattag
     2101 taaggtgaaa atagattcgc atattgctgc ttacaaaaag aaaatgtttg ccaacaattg
     2161 ggccgaaatg ccacaatatt ttgttacttc agcaacagaa atgaccggaa aagatgaggt
     2221 tttgactttt attgatgaag taaatcaaga ggttttcaag aataatagtg agttttaaaa
     2281 gataaattcc aaaagatatg aatcccaaat tccaattaca ataaattgaa atttgggatt
     2341 ttttattttt ttatttcgcc ttcaactcca atttctctgc aaaataatcg cagaaatctt
     2401 gcattgtagc cgacattttt tcatcgccag ttgctcgctg aaacgtttcg gtcatagaaa
     2461 gcaaggtttg atgaaagaat attttcattt catcgaccga catgtctttg gtccacaatt
     2521 cgatacattt tgtagctctt tcatcgctgt tccaaaccga aagcataaag gcttttgcac
     2581 tttctaattc tacaccacct tcaacggcag cccatgataa tttttcagga acacgatttt
     2641 cgtctaattc gacatttatt ttaatttcgg aggtgttttt attcgccatc ttttttgggt
     2701 ttgtatttag atttttcaaa taattctttg gcattcattt gcaacatttt ttctaacgga
     2761 acatcattgt ttttcataaa agaacgcacc atttgccagc ctatccaagt gccaactctt
     2821 cctggagatt cgttgtcaat ttctagataa aacttagaaa agggagcagg atttatgaag
     2881 cgattattta gtttttgatc atcgctatac agcatttggt tttctagaaa ataccgccac
     2941 atataccctt cgttttctac acaccatttg ttttgttcga gcgtgtatcc tattttgtcg
     3001 gcatcgctat attctggcaa aagtatgtct tttagataca attgtttccc gaaataaacc
     3061 atttgactca acaaattttt gttggtaatt ggtgcgattt tttgtatcga aaaactcgaa
     3121 acaacatcag gaagaatttg ttttggttcg aaattttgtt tcaaataatt ggggaattgg
     3181 taaaatttat ggtcttttcc caaatataat tctagtgaaa tgatgactaa actatcagtg
     3241 taaatcactt tgttattata atccatttca gagattacag tgattacttt tggcgttttg
     3301 ttctcaggga aataatattt aatatgtttg aaaagcgaat ttagctcttt tgtaactggg
     3361 ccaaaatcag aatatttttt ttgaacctcg gtgtataatt ctctccacaa aggattttgc
     3421 attttttcta gccaaacact atcatcattc cctggcggaa aaaagaaagg gtattgtttt
     3481 tttaattggc ccaaattttt gggttctgtc tcgaaaaata atttgtcaaa acgttctacc
     3541 ttaatttcta ctggaatttc tgcgaccgtt ttttcgattt ttgttttctt atcgcaacaa
     3601 acgaaagcga atgttagtgc aattactata attaattttt tcatttttat tttttttgaa
     3661 actatatcga tgtaaaaggg ttacttttca caaaaaagaa aagaataaaa aggataacga
     3721 taatagctcc tgaaaattat tatttttgca aatatacaat acctacattt taaaaaccca
     3781 aactattatg tctaaaataa gtacccttca ggttgaaaat gcaaacgcat atattgtgaa
     3841 ttggttaaaa acctatgctg aaaatgcaaa agtaaatggt tttgttattg gaatttctgg
     3901 cggagttgat tcggctgtga catctacact ttgtgcacag acgggtttga ctactttgtg
     3961 tgtagaaatg cctattcatc aagcgccaag tcaagtgagt cgggggcgag aacatattga
     4021 gcaattgaaa aaaagatttt caaatgtgag taatgtagag gctgatttga ctgctgtttt
     4081 tgatactttt aaaactattg ttcctcaagt tgataatttg gatatagtaa atttgtcttt
     4141 ggctaacaca cgtgcccgaa tccgaatgac aactttgtat tattttgctg gaattcacgg
     4201 acttttggtg gctggaacag gaaataaagt agaagatttt ggagttggtt tttataccaa
     4261 atatggagat ggcggagtcg atttgagtcc aattgccgat ttaatgaaat cggatgttta
     4321 tgctttggga agttatttaa aaattccaaa ttcaattttg caagcttcac caaccgatgg
     4381 attgtttgga gatgatcgaa cagatgaaga tcagcttgga gctagttatg acgaactaga
     4441 gtgggccatg ctcgaagacg ataaaggaaa tgtttctgat aatttttctg gacgagaaaa
     4501 agaagtattt gatatttata agaaattgaa tacaataaat caacataaaa tgactgaaat
     4561 accagtttgt tgcatcccaa aagattttaa gtagtatttt tctattgatt agtaaaaaag
     4621 taaagaattt tttattaact ttactcattc gtaagtactg caattctaaa atttaatatt
     4681 atgataaaag tttgtttagc aaataatcat cccgtagtgc attttggggt aaaatcgtac
     4741 tttaaagatc acgatgatat ttcgattgta gccaacgttg ggaatttttt aatggtaaga
     4801 gacattctcc ttacaaagga gattgatgtg ttgatcttag atctagaatt agagggtcta
     4861 gcaagtattt ttgaggtcaa aaatatttta aagaattttc ccaaaaccaa gattatcatt
     4921 tttagcgatt tatcagaaca aatatatgcg ccaaatgcaa taaaagcagg ggtttcagga
     4981 tatgtttctc agaaagagaa attagaaact ttgggtcaag caattgtgaa ggtcaatcaa
     5041 ggaaagataa ttattgatga aacggttaag aaaaatcttg ctctaattgc taagcaaaac
     5101 aaaagcgagc gcttgtatcg taaactgtct aatcgtgagg tagaagtttt acgctatttg
     5161 agcgatggta agaaaaacaa tgagatttct aaaattttgg gacttaatga aaaaacaata
     5221 agtacttata aattaagatt attgcaaaaa ttgaatgtta ccaatctagt ggatttggta
     5281 aacaaagcca aaacattaga aatcgtttaa ttgctataac gggacattac tctacctaat
     5341 tttttcgaaa atgaattaat taattttgag taatccattg ccattgacaa aggctcaata
     5401 ttattagaat ctggttctga tgatattgag ttttttattt cttcgatgat tttatctact
     5461 aaataccatc ttagggagag aatggtttca gaaacatatt gacttattgt ttcatttttc
     5521 gtttttggaa aaatgttttg tccttcccaa ttgtgtaaaa ctactctttc gtctgccatc
     5581 aaaatatcgg tcacttcttg tgcaaagtca ggatgcaagt gcattaaata ctgttcaatg
     5641 ctaaagtttt cgttttgatg aaaatagttt atcaagttgt tgtaaatgtc tctaaataaa
     5701 ggatttgcaa gttctacttc gtcttcttgt aaactcaaat agatgcgttg aaatactttg
     5761 tactctttct tttctgtcac attttcgata tctccagctt catttgtttt caaaagtacg
     5821 tcttcgaatt cctcagtttt actgccgtac aacaaaagaa tttcgataat tttccgttct
     5881 aatccataga gaatatctac ttttcggact tctactggcg cttcattttt tacaacttca
     5941 aatgcttttt gttcttgttt ttgttttttt ccaagttcag tgacgtcttt ttgaactaat
     6001 tgtgccaaag tactcaccaa gacttgctcg gaaatatcca taattcgaga acattcttga
     6061 atataaattt cgcgttgaat tctatccgga atttttgaaa tactcaccac catatctcga
     6121 attaaatcgg cttttttgat aggatcgttt ttggcttcat tcatcaacat tgatgccttg
     6181 aattgaatga aatctttaga attttcgtct aaatatttca ctaattcttc ataaggtgtt
     6241 ttcttagaaa aactatctgg atcttctccg tctggaaaag cgcacacttt tacattcatt
     6301 ccttcttcca aaatcaaatc gattcctcga atagaagcgc gtaatcctgc agcatctcca
     6361 tcaaaaagga ccgtaatgtt tttggtcaat cggttgatta atcgaatttg atcgggcgtt
     6421 aaagccgttc ctgaggaagc cactacattt tcgattccag cttgatgaaa ctgaatgaca
     6481 tcagtataac cttcaactaa atagcaattg ttttctttgg caatggcttg tttggcttgg
     6541 aaaattccgt acaaaacttt gctcttatgg taaatctcac tttcgggcga attgagatat
     6601 tttgccgctt ttttatcatt agtcaaaatt cgacctccaa aacctaaaat ccttcctgac
     6661 atactttgga tggggaacat cactcgtcct ttgaaacggt cgaatggcct gtcatctcta
     6721 ggaattgtca atccagtact ttcaagaaat tctaatttat aaccttttcc caaggcttct
     6781 ttggtcaatg cgtcccaggt ttcgggcgaa taaccaagca caaatttctt tatagtttcg
     6841 ccagtaaaac ctctttcttt gaagtaggaa agaccaatgg ctttgccttc ttctgaatgt
     6901 aaaagggtgt tgtgaaaata atctttggca aattcagaaa ccaaatacat actttcgcga
     6961 acatcggtat tggctttttc ttcgtcggtt tgttcggttt cttcgatttc gatattgtat
     7021 tttttagcca aatatcgaat ggcttcagga taagtaaaat gggagtgttc cattaggaaa
     7081 gctatggcat ttcctccttt tccagagctg aaatctttcc aaattccttt ggctggcgac
     7141 accataaacg atggtgaacg ttcgtctgaa aacggactca atcctttgaa attacttcca
     7201 gcacgtttta attgtacaaa atcgccaata acttcctcaa ctcgagcggt ttcgaaaaca
     7261 gtgtctatgg tggcttttga aatcaatttt tatgatgtta aatgttggaa agcttcaaag
     7321 atacaaagta aatcaatttc aatttcaatg gcaatgtcaa aattcaattg taatagcata
     7381 tctattgata ttgcaattga attttgacat tgaaattgat gtttatctta attttttcaa
     7441 agaaataaat caagatgaac tattaattaa aatcaaatcg aatacctaat gaaatattat
     7501 tctgatctac tggattattc ttgaataatg gatttaaatc gtatttcaaa taaaggctag
     7561 tttctttata accaagataa gtactcacac cataaataaa atcattggca ttgaaatcgc
     7621 ctttggtttt tattcttatt ttatcatcat caatgttata atacattttt tgtttggatt
     7681 taattcggaa acctccgtag cctccaattc cgaagcgtac tcctttatgg gtcgaaaagc
     7741 cagtacttcc atttttgaat ttatttcctg aaaaatcaaa ctctaggtgc aaaggagcaa
     7801 ccagatagac atttttaaaa cgagaatcct ctaatttata ggggaaaacc tcaagattag
     7861 tttggttatt tgtagttgga acaaaataac gattatcagt agggcaaatg ttattgtaca
     7921 ttaccgaaaa accgtatttt aagtgtaata aattataatt tcggaatatt cgggtgttgt
     7981 aggtaaaccc ccattcaaaa aaatgtgatt gccaataata gaaatcagag ttggcgactg
     8041 cttcatccgt aactagatta ttagctccaa aagcaagaac aaattgtgac gtacttcttt
     8101 tttcgatttt gttattggaa tttattattt tcattgatgg aataacgaaa gtttttctac
     8161 ctgatgaatc taacgaagta atatttccat caactttctc ttgaactaaa tttttaaggt
     8221 tttcctgttc gacagctact ctagcttcaa tgtttttagc tctattttca gctaattttt
     8281 gtttctcgat agcagctttt tctttagtaa taagtccatt ttctaattga atatttaccg
     8341 cttcgacttc accttttaaa gctgattttt cttctttcgt tatggtttca atctttgtgg
     8401 caattaattt cacttgtttt tcaaaagttc gattctcctg atctccttta tgaatcgagt
     8461 cttgagcttg tattttgctt atgcaaagca acataaatac tgtaatgtaa aagataattt
     8521 ttgtcatgat gaaagggttt taattgttcg atttttgatt gatgatgatt attgattgtt
     8581 tctattgacc aaagcttctt tggcttcttt gtattttttt gtgattgatt gcaatgcttt
     8641 ttctcgaaac gaaagttgta attctccgtc aacttgattt agtaatgtgt ttgaatttat
     8701 tttaatagac gattttttaa aattcgaatt tgatttttcc tctactgaag ctaataaaga
     8761 atcaatattt gtttgaatat catttttgtg ttctactatt agtaagcttt cattttttgg
     8821 atgagtttca gctaaaacat taccttgagt tttaagttct agattttcga ctttggggtt
     8881 gttgctcctt tgagtttgaa ccaaaggttt tgtttctgaa tttaaaatgg ttttaggaaa
     8941 atccgactct tgttttgtcg acttaattcc gttttttgaa ttctcttttg ggctagattt
     9001 ttcaattata gcggtattaa ttttggtgtc gattgggtaa tctttttgat ttaaaaaaac
     9061 agttgctatc aaagataaac ctataaaact tgccgctata taaacccagc tgtatttagg
     9121 ttttgatttt ttttcttcgg caacagataa cattgcgttc aatcgatccc aagccatatc
     9181 tgttggtttg atttcacgag aattcaattt ttctttgaac tgtttttcta ctttattccg
     9241 ttccattgct gtaatttttt agcgtcaata tttgctcttt caaaattttt cgggcgtgag
     9301 acaattgcga tttagaagtt ccttcgttaa tgcccaacat actcgcaatt tcttggtgtt
     9361 tgtagccttc aatcgcatac aaattgaaaa tcattcggta accatcgggc aagttgtcta
     9421 tcaaaaactg aatgtcttct accgaaaatt ggctttctat attattgaaa ctttcttcaa
     9481 aataattctc atcctcaaca aaacttactt ttttttgaac tcggatattc gaaatacatt
     9541 cgttaaccat aattcttcga atccaacctt caaaacttcc tttttgctca aaatttttca
     9601 gattagtaaa taccttcatg aaagccgtaa tcataatgtc ttcggcttgg tgaatgtcct
     9661 ttatatactg tcggcacaca cttaacattt ttggagaaaa tttggcgtaa attttttgct
     9721 gggcagctcg attgttctcg attgccaaac gaatgatttc attttcttct tggttaaagt
     9781 gaatgacttt caattttgtt ttataaagtg ttgctataag taagacgaga ctgcttgcaa
     9841 aaaggttgca tcagaaataa aaaaaaacag tatgaaaaat aaaaacctca ataaaatcaa
     9901 ggtttcagag ggcgtgtttt attaaaaaaa aattattttt ttctttcgat aacatagttt
     9961 accatcaaaa ttaaagcgtc tttaaactcg gattgtgcat aattatccag aagtgacaat
    10021 gcttcctgtt gaaattctgc cattttttgt tcggcataga acaagccgtt atggtttttt
    10081 acaaaagcaa ttacttcttt cactcgtttt ttgtctttgt tgtagttttt gatggaattg
    10141 ataacccaag atttttcttt tggagtacaa ttattcaaaa catgaatcaa tggcaaagtc
    10201 attttttgtt ccttgatgtc aattcctgta ggtttgccaa tggcatcctc agtataatca
    10261 aacaaatcgt ccttgatttg aaaagccatc ccgatgagtt cgccaaattt tcgcatattc
    10321 tctacctgaa tttcatcttc aatcaccgat ttcgctccca aagcacaaca agcagcaata
    10381 agtgttgctg tttttttgcg aatgatttcg tagtaaacat cctcggtgat atcgagtctt
    10441 ctggcttttt ctatttgcaa taattcgcct tcactcattt cgcgaacagc taccgaaatg
    10501 attcgcaata aatcgaaatc gccgttatca atggaaagca acaatccttt tgaaagtaaa
    10561 taatcaccaa ctagtacagc aattttgttc ttccaaagcg cattgatgga gaaaaaacct
    10621 cttcggcgat tgctgtcatc tactacatcg tcatggacta aagttgcggt gtgaattaac
    10681 tctataaccg aagctccacg ataggttctt tcgtttacaa ttccagccga aaccattttg
    10741 gctgtcaaaa aaacaaacat cggacgcatt tgttttccct ttcgattcac tatataatag
    10801 gtgattcggt tcagcaaagc cacttttgaa gtcatcgatt catagaactt tttttcgaaa
    10861 agttccatct cgttaaaaat aggctgcttt atttgagaag tgatgttcat ttaggattca
    10921 aatatactat tttcgaataa tataatggca tcaaattttt gttattgaat gtgttttttg
    10981 aaacatttta ttttacctaa aatgaaatta gattagagtt tcttttcggt ctaaatgtgt
    11041 tatgatttca gaatttgtgg ccttttattg agagattatt attcttacat aattgatatt
    11101 tagtcaaata aaaaaatctt ttgagagtgt agttcatcga atattggctt aaatgttgtc
    11161 cttttttgca tttttattta taaatttaga atcccaaatt aaacctttta ttgatttcat
    11221 aatcaaatta ttaaacctaa aaaatgaatt ttatgaaagc caaaatttta ttattcgtta
    11281 ccatgttgtc cattgtaact tttgttggtt gtaattccaa tgaaactgta gatggatcaa
    11341 cgactacagc tgcaattacg gctgatgaga ctgttgtaaa ttcagaaatt gatgctacgg
    11401 tagatgacgt ttctattatt gcagaagatc agtatgacgt tcaaaaaagc gcgtcttcaa
    11461 ctgcaaaaac aactgctgga atggtaagta tgttgccagc ttgcgcaaca attacaaaag
    11521 tattgacaaa tgacacttgg accagaacta ttgattttgg aactcaaggt tgtgctttgc
    11581 caaacgggaa tatcgtaaaa ggaaaaatta tcattagttt ttctaagaat tttacaactc
    11641 caataagaac gatttcctat actttagaag ggttttatca taacgataaa ttaattgaag
    11701 gaagtaaaac cattactcat gaacttaaaa cttcagattt attggcaact attcatcctg
    11761 ttacaacaca ctcagttgat gtgaaaatta cttatcctga tggtaaaatt tatactagaa
    11821 ttggaacacg agttagagaa atggttgaag gatttgcaac tattactaat tgggaagata
    11881 atgttttcaa agtttgggga tacaatatca ctacttttcc gaatggttca aaat
//
LOCUS       DAOSNN010000049        11784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5405_length_11784_cov_4.491261, whole genome shotgun sequence.
ACCESSION   DAOSNN010000049 DAOSNN010000000
VERSION     DAOSNN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5405_length_11784_cov_4.491261"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLQ70_04050"
     CDS             complement(<1..261)
                     /locus_tag="PLQ70_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQE33821.1"
                     /translation="MSTILEVNKVVKKYGDFVALNEVSLTVPKGSIYGLLGPNGAGKT
                     SLIRIINQITMPDSGEIILDGENLSPKHIQNIGYLPEERGLYK"
     gene            complement(405..1517)
                     /gene="dnaJ"
                     /locus_tag="PLQ70_04055"
     CDS             complement(405..1517)
                     /gene="dnaJ"
                     /locus_tag="PLQ70_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQE33822.1"
                     /translation="MKKDFYEILGISKNADAAEIKKAYRKKALEFHPDKNPGNAAAEE
                     SFKAAAEAYEVLSDPQKKAKYDQYGHQAFDGSGGFGGGGHGSMNMDDIFSQFGDIFGG
                     GFGGGFGGGGGVRRAKGTNLRIKVKLTLEEIANGVEKKVKVKRKVQAKGVSYKTCSTC
                     NGQGQVMRVTNTILGRMQSASTCPTCGGSGQILDKKPSEADSQGMVVEDETVTIKIPA
                     GVVDGMQLKVSNKGNDAPGNSVPGDLIVAIEEIEHEFLKREGENLHFDLYISFSEAVL
                     GISKDISAVNGKVRIKLDEGIQSGKILRLKGKGIPNINGYGSGDLLVHVNVWTPKTLN
                     KEQKQFFESNLENENFIPNPEKSDKSFFEKVKDMFS"
     gene            complement(1593..2192)
                     /locus_tag="PLQ70_04060"
     CDS             complement(1593..2192)
                     /locus_tag="PLQ70_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQE33823.1"
                     /translation="MKFKNIFKNNTRMATENKVNDEIVDQAPIENNENNTDALDASIE
                     ERVEEISREEQLSQDLASEKDKFLRLFAEFENYKRRTAKERIELFKTANQDVLLAMLP
                     VLDDFDRALAEIKKTDDNILIQGVELIQEKLKNTLVSKGLEQVELQAGDAFDADFAEA
                     ITQIPAPSPKLKGKIVDVIEKGYKLGDKIIRFPKVVIGN"
     gene            complement(2508..2960)
                     /locus_tag="PLQ70_04065"
     CDS             complement(2508..2960)
                     /locus_tag="PLQ70_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HQE33824.1"
                     /translation="MKFTVSNLNKFLFFKLPSAYISGVRVKQIEANKCVVTVKHRWIN
                     QNPFNSMYFAVQAMAAELSTGALVMNHVQNSGKKISMLVANNKSNFTKKATGRITFEC
                     LDGDLAEKSIQDAIVSGEGKTFWMKSIGTNENGEQVSEMDFEWSVKSK"
     gene            3195..3650
                     /locus_tag="PLQ70_04070"
     CDS             3195..3650
                     /locus_tag="PLQ70_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HQE33825.1"
                     /translation="METTNNKNLAAFTHLSTLTQYCIPFGNYIFPILIWSSNKSKSEF
                     IDFNGKQVLNFQLSMFLYSLILAVIAIPILIITFFNNVPLHTLIDEEVFMRNHFSVEN
                     ITGIAIVAIVAILLFVGLKVAEFFLIIYASVKASNGEKFEYPLTIPFLK"
     gene            3712..4044
                     /locus_tag="PLQ70_04075"
     CDS             3712..4044
                     /locus_tag="PLQ70_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQE33826.1"
                     /translation="MNIENTKAQMRKGVLEFCILSVLKDKEAYTSEILDTLKNAKLLV
                     VEGTVYPLLTRLKNDGLLSYRWEESLSGPPRKYYGLTEIGHTFLNELNGTWTELSDAV
                     NLITTKKQ"
     gene            4047..5771
                     /locus_tag="PLQ70_04080"
     CDS             4047..5771
                     /locus_tag="PLQ70_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HQE33827.1"
                     /translation="MNKTVNINLGGMFFHIDEDAYQKLTRYFDAIKRSLSNSSGQDEI
                     IKDIEMRVSELLTEKQKTEKHVVGLKDVDEVIAVMGQPEDYRIDEEGIGNTTTNYSSS
                     SSTGNRKLYRDKDNGMIGGVLAGLGHYFGIDKVWLRIFFLIMFFAWGTGVLAYIILWI
                     VMPEAKTTAEKLEMTGEPVTISNIEKKVREEFENVSEKIKNADYDEMGNRVKTGAEKI
                     GSSFGDFIIAVFRVFSKFLGVILIIAGLFILAALFIGIFVLGSTSFFHYPWSNFIEAG
                     NFTEYPVWIFGAIMFLAVGIPFFFLSLLGFKLLSPNLKSIGNIAKYTLLAVWLIAVSL
                     AIAIGLKQASAFAIDGRVVVKENIILKPTDTLLLKFKHNDYFAKNLYDNDNFKITLDS
                     TGNNILYSNNVSFKIAQSDTQSAYIQIEKEAKGENLFEAKQRAEKIKYSYKVIGNQII
                     FDNYLLTDVKEKFRDQEIEITLFLPLGTLLKMDESMKNYDQTDGDYFLWNPENKNEVY
                     KVESDKIKCQNCFNEGDENVTIDTKQEADSVVTTTVTVNGEVVKVKETTTKKGLSVDE
                     DGVIVKNK"
     gene            5774..6511
                     /locus_tag="PLQ70_04085"
     CDS             5774..6511
                     /locus_tag="PLQ70_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HQE33828.1"
                     /translation="MIKIITIITKFIIAALMALLFASCNHSFNLESGIKGSGNLTTET
                     RVADQDFKNIEVSSGIKVFVEQADIKSITVETDDNLQEHIITKIENGVLKIEANKGYN
                     STTPMVRVKMPIINGLSASSGSKINSSYTLTSNNIKANSSSGSLINIDIEADAITLES
                     SSGSEIEARGKALKLETNSSSGSHINAKALMANEVFSQSSSGSSTSVYPILKLDAKVS
                     SGSSIDYYKVPKTLSKDISSGGSIDEK"
     gene            complement(6742..7689)
                     /gene="trxB"
                     /locus_tag="PLQ70_04090"
     CDS             complement(6742..7689)
                     /gene="trxB"
                     /locus_tag="PLQ70_04090"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQE33829.1"
                     /translation="MSDTIEKIKCLIIGSGPAGYTAAIYAARANMNPVLYQGMQPGGQ
                     LTTTNEVENFPGYVDGVTGPEMMIQLQAQAQRFGADIRDGWATKVDFSDGIHKVWIND
                     TIELHCQTVIISTGASAKYLGLESEQHYLKMGGGVSACAVCDGFFYRNQEVVIVGAGD
                     SACEEAHYLSKLCSKVTMLVRSDKFRASKIMEERVRKTENISILMNHDTVEILGDGQV
                     VTGVKAKNKTTGDIFDIPATGFFAAIGHKPNTDIFANYLTLDETGYIVNIPGTSKTNV
                     AGVFVAGDAADHVYRQAITAAGTGCMAALDAERYLATLE"
     gene            complement(7816..7893)
                     /locus_tag="PLQ70_04095"
     tRNA            complement(7816..7893)
                     /locus_tag="PLQ70_04095"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7856..7858),aa:Pro,seq:tgg)
     gene            7966..8598
                     /locus_tag="PLQ70_04100"
     CDS             7966..8598
                     /locus_tag="PLQ70_04100"
                     /inference="COORDINATES: protein motif:HMM:NF033708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS sorting signal type C domain-containing
                     protein"
                     /protein_id="HQE33830.1"
                     /translation="MVKIECNYKAITFNIQIGVDAFDGKYYNDSEFALNTLLDNEEYV
                     IQGRPLPFDATDKVPLVFKTTNAGNYTIAIDHTEGLFSANQDIILVDHLTGIETNLKS
                     SEYTFAAVAGVDNSRFSLKYQKTLGNHQQVLDENNILVYKSKGAIHIKSGKTNIDNLK
                     LFDISGRFIFEKKNIKSQETSIENAKLGNQILVVKITLENQKVVSKKIEN"
     gene            9655..>11784
                     /locus_tag="PLQ70_04105"
     CDS             9655..>11784
                     /locus_tag="PLQ70_04105"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HQE33831.1"
                     /translation="MRKPETFIKEKLLYAFLFLFTISILASEKPIKTEKPVKTEKPAK
                     AAKTAATTTLTFVSETILPTTILEPSDLAFDKVNNVLYTVNDNNGKVYKLSTSGAILQ
                     TLSFAGADLEGVCMYKNNKILIGVEGTKQLIEYDYVTDTQGATHNMAYVHESTDTNSR
                     LEGVSYDAVHDVMYMTNEKNPTYLIVANGSFGITNEIALPDGGGTIDYSACHYVEETG
                     DLWMASDKASSIYRCNTIGTKLETFAVKNKLSGGATLNKLEGITIDYPNQILYGITDG
                     SHAFYIYKIDDGQPTRTWTGAIDSDWSKPGNWFPASVPYTAAQITIPVTANQPIINAD
                     FIVSSLTINSGATLTINAGKTLTNKGTISNLGTINNLGTINNTAGSLVLKSNATGTGS
                     LLSDTSVTNVSQQHYLTSNQRGWRLLSNPLATITFGTLASGSTTPITLGTNATGEYFP
                     ATNSWTFSASGDANSMTSQKAYEVFIRGRATEVTGLNYSVNPPSNVTLSITGTAANTV
                     PSSISTTAGRYYLVANPYTAPISVANLLSASTGLSNSVSYYNPTQGSNGTTDLIIKKG
                     GYNAVTVSGTAGAATDVVLPPMGAIFVQATTSGSINIPKTTIYTGTVTSPAGNFAHKT
                     ANTSKIIQEALTINVSSSEADFDKLQLKFKAVGSEGSNIDMGKLPNTILDFYSIAEDN
                     QNKAISELELKPQTIPLGINSSALQNFK"
BASE COUNT         4028 a         2089 c         1858 g         3809 t
ORIGIN      
        1 tttatacaaa ccacgttctt cgggcaaata gccaatattt tgaatgtgtt ttggacttag
       61 attctcgcca tctagtataa tttcgccact atcaggcatt gtaatttggt taataatccg
      121 aataagtgat gttttcccag caccatttgg tcctaaaaga ccgtaaatac ttcccttggg
      181 aacggttaaa gaaacttcat tcagggcaac gaaatcgcca tattttttaa caactttgtt
      241 tacttcaagt atagtactca taaaggtatt ttgattattt tagctattta accctttggc
      301 gttgggtgtg taaaagtaga aaaatagtgc taataaggtg ttaaataaaa caaaaaaacc
      361 cacccttgtc atacaaggat gggaaaaatt gacataatta aaaattatga aaacatatct
      421 tttacctttt caaaaaagga tttatcactt ttttcaggat ttggaataaa attttcattt
      481 tcgagattgc tttcaaaaaa ctgtttttgt tccttattga gtgtttttgg cgtccataca
      541 ttaacgtgaa ctaacaaatc tcctgaacca taaccattga tattcgggat tccttttcct
      601 ttcaatcgca agattttacc agattgaatg ccttcgtcta attttattct cacttttccg
      661 tttacagccg aaatatcttt tgaaattcca agaactgctt ctgaaaaact gatgtataaa
      721 tcaaaatgca aattctctcc ttcacgtttt aggaattcgt gctcaatttc ttcgattgcc
      781 acgattaaat ctcctggaac gctgtttcct ggtgcatcat ttcctttgtt tgaaactttt
      841 agttgcatgc catcaacaac tccagcagga atttttatag taaccgtttc gtcttcaaca
      901 accattcctt gagaatcagc ttcggatggt tttttgtcta aaatttgacc tgaaccacca
      961 caagttgggc aagtagatgc tgattgcatt cttcccaaaa tagtgttggt cacacgcatt
     1021 acttgacctt gaccgttaca agtagaacag gttttataag aaactccttt tgcctgaact
     1081 tttcgtttta cttttacttt tttctcaact ccgtttgcaa tttcttcaag agttagcttt
     1141 acttttatac gaaggttagt tccttttgca cgacgaacac cgcctccacc tccgaaacca
     1201 cctccgaaac caccaccaaa aatatctcca aattgactga aaatatcatc catattcata
     1261 ctgccgtgac ctccgccacc aaaacctcct gagccatcaa aagcttgatg accatattga
     1321 tcgtatttgg cttttttctg aggatcactt agtacttcat atgcttcggc agctgctttg
     1381 aaactttctt ctgcagcagc atttcctgga tttttgtcag ggtgaaattc gagtgctttt
     1441 tttctatatg cctttttaat ttcggcagca tcagcatttt tggaaatgcc tagtatttcg
     1501 taaaaatctt ttttcataat taaaataagt tccaaatttt aaaattctaa atgccaattg
     1561 cttagtcgat ttagatttat gatttgattt ttttagttgc caattactac ttttggaaaa
     1621 cgaataattt tgtctcccaa tttgtatcct ttttctatta catctacaat ttttcctttc
     1681 aacttaggcg atggtgcagg aatttgagtg atagcctcag caaaatcggc atcaaaagca
     1741 tcgccagctt gaagctctac ttgctctaat cctttagaaa ccaatgtgtt ttttaatttt
     1801 tcttgaatta attcaacacc ttgaatcaaa atattatcgt ccgttttttt gatttcggcc
     1861 aatgctctat caaaatcgtc taaaacaggt aacatagcaa gtaatacatc ttgattggca
     1921 gttttaaaca actctatacg ctcttttgct gttctgcgtt tgtagttttc aaattcagca
     1981 aataagcgca agaatttatc tttttcagat gctaaatctt gtgacaattg ttcttctctg
     2041 gatatttcct ctactctttc ttcaattgaa gcatctaaag catctgtatt attttcattg
     2101 ttttcaattg gagcttgatc aacaatttca tcgtttacct tattttcagt cgccattctt
     2161 gtattgtttt taaatatatt tttaaacttc attttttatt cttttctgtt gattgcaaaa
     2221 gtactgccaa atcttctaaa atgtcaaatt gtcattaatt aattattata tgatgaatgg
     2281 ctatataatt ataggctgaa aatgattctt ttgattgcca aaaaatttaa tataatttta
     2341 agactatcac gatttagttt ctttaaattt gtgaagattc taaataaaaa caaacaaagg
     2401 ttagctcctt tttgggcttt aaattattat taattcacta gttattatag ttaaaaaaag
     2461 cttcgtcccg cagacgaagc ttttttttat gcaatcaaaa aaagaattta tttagacttt
     2521 acactccatt caaaatccat ttctgagacc tgctctccat tttcgtttgt tcctatagat
     2581 ttcatccaaa acgttttccc ttctccagat actattgcat cttgaatact tttttctgcc
     2641 aaatctccat caagacattc aaaagtgatt cttccggtag cttttttagt aaagttgctt
     2701 ttgttattgg ccaccaacat cgatattttt tttccactat tttgaacatg attcattacc
     2761 aaagctcctg tagaaagttc tgcagccata gcttgtacag caaaatacat tgaattaaaa
     2821 ggattttgat ttatccaacg gtgttttaca gtaactacac acttatttgc ttctatttgc
     2881 ttgactcgaa ctccagaaat gtatgccgat ggcaacttga aaaacagaaa tttattaaga
     2941 tttgataccg tgaatttcat tttatttttt ttagttcttg taaaaataac aaaaattgac
     3001 tacacattta ggttcttcct ttattttata aaaatccgta ttagcacccg tattttaaga
     3061 agttaaactt taattgattt aattttttct aatcccaata tgttaatatt ttgttaatat
     3121 acactactat gtgatgcaag atactgtctt ttggatatat atttgcatta gaaattacta
     3181 tatactttta cattatggaa acaaccaata ataaaaacct agcagcattt actcatttga
     3241 gtactttgac ccaatattgt attccttttg gaaattacat ttttccgata ttaatttgga
     3301 gttccaataa aagcaaatct gaattcatag acttcaatgg caaacaagtt cttaactttc
     3361 aactgagtat gtttttgtat tctttaattc ttgccgtcat tgctattcca attttaatca
     3421 ttacattttt caacaatgtt cctttacaca cccttatcga cgaagaggtg tttatgagaa
     3481 atcatttcag cgtagaaaac attacaggaa tcgcaatcgt agccattgtt gccattcttt
     3541 tgtttgtagg cttgaaagtc gcagagtttt tcttaattat ttatgcttcg gtcaaagcat
     3601 cgaatggaga aaaatttgag tatccgctca caataccctt tctaaaataa tcatcaataa
     3661 tcaatcaaaa aatgaacagt tcaatccaat caaaaaacaa aaaagagagt tatgaacatt
     3721 gaaaacacaa aagcccaaat gcgcaaaggt gttcttgagt tttgcatctt gtcagtatta
     3781 aaagacaaag aagcatatac ctcagaaata ttagacactt tgaaaaacgc aaaattactg
     3841 gttgtggagg gaacggttta tccgttgctt acccgattga aaaacgacgg tttacttagc
     3901 tatcgttggg aagaatcgtt gtcaggtcca ccaagaaaat attatggact gaccgaaatt
     3961 ggacacactt ttttaaacga attgaatggc acctggactg aattgtctga tgcagtaaac
     4021 ctaatcacaa ccaaaaaaca atagccatga acaaaactgt aaacataaat ctaggcggaa
     4081 tgttcttcca tattgatgaa gacgcatacc aaaaactaac gcgatatttt gatgccataa
     4141 aacgctcttt atcgaattca tctggtcagg acgaaatcat aaaagacatc gaaatgcgtg
     4201 tttcggaatt attgaccgaa aaacaaaaaa cggagaaaca tgttgtaggt cttaaagatg
     4261 ttgatgaagt tattgcggta atgggtcaac ctgaagatta tagaattgac gaagaaggta
     4321 ttggcaatac aacaactaat tattcttcaa gttcctcaac aggaaatcga aaactatatc
     4381 gcgacaaaga caacggtatg ataggcggtg ttttagctgg tttaggacac tattttggaa
     4441 tcgataaagt ttggttgcga atcttctttt taatcatgtt ttttgcttgg ggaactggag
     4501 ttttagccta tatcatcctt tggattgtaa tgccagaagc aaaaacaaca gccgaaaaac
     4561 tagaaatgac tggtgagcca gtaacaattt cgaatattga aaaaaaagta cgtgaagaat
     4621 tcgaaaatgt ttcagaaaaa atcaaaaacg ctgattatga tgagatggga aaccgtgtaa
     4681 aaacgggagc cgaaaaaatt ggtagctcgt ttggggattt tataatagcc gtatttagag
     4741 ttttctcaaa attcctaggt gtgattttga ttattgcagg tctttttata ttggcagctt
     4801 tatttattgg aatttttgtc ttgggtagca cttcattttt ccattatcct tggtcaaatt
     4861 tcattgaagc tggcaacttt acagaatacc ccgtttggat atttggtgca attatgtttt
     4921 tggcagttgg aattccattc tttttcttgt cattattagg tttcaaatta ttatcaccca
     4981 acttgaaatc tattggaaat attgcaaaat ataccttgct tgccgtgtgg ctaatagcag
     5041 tttcattagc aatcgccatt ggattaaaac aagcttctgc ttttgcaatt gacggacgag
     5101 ttgtagtaaa agaaaacatt attctgaaac caacagatac acttttgcta aaattcaaac
     5161 acaatgatta ttttgccaaa aatctgtatg acaatgataa ttttaaaatc actttagatt
     5221 caactggcaa caatatcttg tattctaata acgtaagttt caaaattgcg caatcagata
     5281 cacaatctgc ctacatccaa attgaaaaag aagccaaagg cgagaactta ttcgaagcca
     5341 aacaaagagc cgaaaaaata aaatacagtt acaaagtcat tggtaatcaa ataatttttg
     5401 acaactattt attaaccgat gtaaaagaaa agttccgtga tcaagaaata gaaataactt
     5461 tattcttacc tttaggcact ttattgaaaa tggatgaatc tatgaagaat tatgaccaaa
     5521 cggatggcga ttatttttta tggaatccag aaaataaaaa tgaagtttac aaagtagaaa
     5581 gtgataaaat aaagtgtcaa aactgcttta atgagggcga cgaaaatgtc acaatagata
     5641 cgaaacaaga agctgatagc gttgttacta caacagtaac tgtaaacgga gaagttgtta
     5701 aagttaaaga aacaaccaca aaaaaagggt tgtctgttga tgaagacggt gtaatagtta
     5761 aaaacaaata gtcatgatta aaattatcac gataattaca aaattcataa ttgccgcttt
     5821 aatggcctta ttatttgctt cctgcaatca ctccttcaat ttggaaagtg gaataaaagg
     5881 aagtggtaat ttaaccaccg aaacaagagt agctgaccaa gatttcaaaa atattgaagt
     5941 aagtagtggc attaaagttt ttgtggagca agccgacatc aaatcaatta cggttgaaac
     6001 cgacgataac ttgcaagaac acattatcac caaaatagaa aatggagttt tgaaaattga
     6061 agccaacaaa ggatacaatt caacaacacc tatggttcga gtaaaaatgc ccattatcaa
     6121 tggattaagt gctagtagcg gttcaaaaat aaatagttct tataccctaa catccaacaa
     6181 tataaaagca aattcaagca gtggaagcct cataaatatt gatattgaag ctgacgcaat
     6241 cacactagaa agctccagcg gaagcgagat tgaggctcga ggcaaagcat taaaactaga
     6301 aacgaattcc tcaagcggaa gtcatattaa cgcaaaagct cttatggcaa acgaagtctt
     6361 ttcgcaatcc tcaagcggaa gtagcacctc ggtctatcct attctcaaac tagacgcaaa
     6421 ggtatctagc gggagttcta ttgattacta taaagttcca aaaacgcttt caaaagacat
     6481 ctcttctggc ggaagcattg acgaaaaata aatccttttt acctaaaaat catccatcaa
     6541 aaatgggtga ttttttttgt ttaatagtca aaaaagttgg taaaaatact gtaagacact
     6601 ataaaatata atgttcccga aagtttatta aaattcgttt ggcaacaatt tagttccaaa
     6661 aaaaataccc ccaaatagtc ttaaactttt tggaggtaat tcaatttttt agcactattc
     6721 aattcaaaac attgcgttaa attattctaa agttgccaaa tatctttcgg catccaaagc
     6781 agccatacat cctgttcccg cagcagtaat tgcttgacga tatacgtgat cggcagcatc
     6841 tccggcaaca aaaactcccg caacattcgt ttttgatgtt cctggaatgt taacgatata
     6901 acctgtttca tcgagcgtta gatagtttgc aaaaatatca gtattgggtt tgtgaccaat
     6961 ggcagcaaag aaccctgtag caggaatatc aaaaatatca cccgtagttt tattttttgc
     7021 ttttacacca gttaccactt gaccatctcc caaaatttcg acagtatcat ggttcattaa
     7081 aatagaaata ttttcggttt tacgaacacg ttcttccata atttttgaag ctctaaattt
     7141 atcacttctc accaacatag tcactttcga acacaatttt gacaaataat gggcttcttc
     7201 gcaagcagaa tcacctgctc caacaataac tacttcttga tttctataga aaaagccatc
     7261 acaaactgca caagccgaaa caccaccacc catttttaga taatgttgct cggattctaa
     7321 acctaaatat ttagcagaag ctcctgttga aataattacg gtttgacaat gtaattcgat
     7381 tgtatcgttg atccaaactt tatgaatacc atcactaaaa tcaacttttg tggcccaacc
     7441 atcacgaata tcagcaccaa aacgttgtgc ttgtgcttgc aattgaatca tcatttcagg
     7501 accagttact ccatcaacat aacctggaaa attttccact tcgttagttg tagtcaattg
     7561 tcctccgggt tgcattcctt ggtacaaaac tggattcata ttggctcttg cagcataaat
     7621 agctgctgta taacccgctg ggccagaacc tataataagg catttaattt tttcgatagt
     7681 atcagacata attaggattg atttttttta acgacacaaa aataagttat aaaagctaga
     7741 ttatggaaat agaataatca ttttttgcta ttcaacaata aaaaaatccc ttctgaaaga
     7801 cagaaaggat ttaagtggtc ggggtggcag gattcgaacc tgcggcctcc tgctcccaaa
     7861 gcaggcgcga taaccgagct acgctacacc ccgaaagcgg tggcaaatat ataactattt
     7921 ttggcttctg caaaacaatt tccaaaataa gaaataaatt taattttggt taaaatcgaa
     7981 tgtaattata aagctataac ttttaatatc caaattggag tcgatgcttt tgacggaaaa
     8041 tattataatg atagcgagtt tgctttgaac accctacttg acaacgaaga atatgtgatt
     8101 caaggacgtc cgttaccttt tgatgctact gacaaagttc cattagtatt caaaactaca
     8161 aatgcgggta attacaccat tgccattgat cacacagaag gattattttc ggcgaaccaa
     8221 gacattattt tagtagacca tttaactgga atcgaaacta acttaaaatc aagcgaatat
     8281 actttcgcag cagtagctgg agtcgacaat tcaagatttt ctttgaaata ccaaaaaact
     8341 ttgggtaacc atcagcaagt acttgacgaa aataatattt tggtgtacaa aagcaaagga
     8401 gccattcaca tcaaatcggg caaaacaaac attgacaacc taaaattatt tgacattagt
     8461 ggccgtttta ttttcgaaaa aaagaatata aaatcccaag aaaccagtat tgaaaacgca
     8521 aaattgggca accaaatctt agtcgtaaaa ataacattgg aaaaccaaaa agttgtcagc
     8581 aaaaaaattg aaaattaaaa aaacagtaat tatctaaaca aatgcagagc attcgccttt
     8641 aatctgggta agattaataa ttataatttt gaagtaaatt gtattcttgc taattgtcat
     8701 gctcgaagcg gctaatttat cttgctctct tgctttgaga cacttccagc gtgacaactg
     8761 gagtagttaa atatatcttc aaataagaat caatttaaac tgttccgccg gctatgtctg
     8821 aaacttttaa aagcgaatgc cctgaacaaa tgagttgtca aaatattttt cattgttgtc
     8881 cgataaaaag cataaataaa atttcgaact caatgtttat agtaaataca tacaggtcgc
     8941 tcctctggag cttgtttttg gcgtcttact ttttctaaaa acagtatgtc ccgatgggac
     9001 taaaatagct ccgataggag ttaactgttt gtaggaaaca gtgatttttg gtaaacaaag
     9061 ctccagagga gcgacctgtt gattattctt aaaattttat cggacaacaa tgaatatttt
     9121 tataaaattt tatctaataa atagttaact cattttagca acttcaaatt tatctaaata
     9181 agtcttaatt tacgacttta taaatcattt cccctactct taatttagca agtaggggat
     9241 ttttttatgc ttcaaactta aatttcaggt taacttattg caaataaata atcataaaat
     9301 attactattc ctacaaaata attagttttt atatatttta tcgataaata tcgcatttaa
     9361 tcgataaaaa attattttgt tttaaaaaac ttttttacat ttgaaaactt aatcggacaa
     9421 atatcaaata attgagtcct tttgagataa aaaaatagat agtaaaaatt ccttattgga
     9481 attataaaaa aacattaatt catcctttta gtcctaaaat catgaaaaaa aatactcttt
     9541 taaagaaaac atttcttagc ctagttttta taaaaaaact atggatttga aaacctcaac
     9601 ttttttataa caatctttta aataaacaac tcaacctaaa ccccaaatac caacatgaga
     9661 aaaccagaaa cttttattaa agagaaactt ttatatgcct tcctttttct ttttaccatt
     9721 tcaattttag ctagtgaaaa acccataaaa acagaaaaac cggtaaaaac agaaaaacct
     9781 gcaaaagcgg caaaaacagc agctactact actttgactt ttgtaagcga aaccatctta
     9841 ccaacaacta ttttagaacc atctgatttg gcttttgata aggtaaataa tgttttatat
     9901 actgttaatg ataataatgg aaaagtgtat aagctatcta catctggagc gatactacaa
     9961 acattatctt tcgcgggtgc agaccttgaa ggggtttgta tgtataaaaa caataaaata
    10021 ttaataggag tagaaggtac aaaacaatta attgaatatg attatgtaac agacactcaa
    10081 ggtgccactc ataacatggc ttacgtgcat gaatcgacag atactaattc acgccttgaa
    10141 ggagtgtctt atgatgctgt gcatgatgtt atgtatatga caaatgaaaa aaaccctaca
    10201 tacttaattg tcgctaatgg tagctttggt attacaaatg aaattgcatt gccggatggt
    10261 ggagggacta ttgattattc tgcctgtcat tatgttgagg aaacgggtga tttatggatg
    10321 gcaagtgaca aagcttcttc tatttataga tgcaatacca ttggtactaa actcgaaaca
    10381 tttgctgtaa aaaacaaact cagtggagga gctacactaa ataaattaga aggcataacc
    10441 attgattatc ctaaccaaat tttatatggc attaccgatg ggagtcatgc tttttacatt
    10501 tataaaatag acgatggaca acctactaga acctggacag gagctataga tagtgattgg
    10561 tctaaaccag gaaattggtt ccctgcaagt gtaccttata cagccgcaca aattactatt
    10621 cccgtaacgg ctaatcaacc cattatcaat gctgatttta ttgtaagttc actaactatt
    10681 aattcaggtg caactttaac tataaatgct ggaaaaacct taactaataa aggcactatc
    10741 tcaaatttag gtactataaa taatttaggt actataaata atactgctgg aagtttagta
    10801 ttaaaatcaa atgctaccgg tactggttct ttgttgagtg atacttcggt aaccaatgtt
    10861 tcgcaacaac attatttaac aagcaatcaa agaggatgga gattattgag caatccactt
    10921 gctacaataa catttggaac acttgcctca ggaagcacta ctcctatcac tttgggaaca
    10981 aatgctacag gagaatattt cccagcaaca aattcttgga ctttttctgc cagtggagat
    11041 gctaactcaa tgacttctca aaaagcatat gaagtattta ttagaggtag agctactgaa
    11101 gtaactgggc taaattatag tgtgaatcca cctagtaatg ttacattatc tattactgga
    11161 acagctgcaa acacggtacc ttcttcaatt agcacaacag ccggacgata ttatttggta
    11221 gccaatccct atactgcacc tattagtgtt gctaatttac tatcagcaag tacagggctt
    11281 agcaattcgg tttcctatta caatccaacc caaggttcca atggaactac tgatttaatt
    11341 attaaaaaag gaggatataa tgccgttaca gtttctggta cagctggtgc tgctaccgat
    11401 gtagttttac ctccaatggg agcaattttt gtccaagcta ccacttcagg aagtatcaac
    11461 attcctaaaa ctactattta tactggtact gttacaagtc ctgctgggaa ttttgcacat
    11521 aaaacagcca acacttcaaa aatcatacaa gaagccttaa ctataaatgt aagtagtagt
    11581 gaagccgact ttgataaatt acaattgaaa tttaaagcag ttggatctga aggaagcaat
    11641 attgatatgg gaaaattacc aaataccatt ttagattttt acagtatcgc tgaagacaat
    11701 caaaataaag caatatcaga attagagcta aaaccacaaa ctattccgtt aggaattaat
    11761 tctagcgctt tgcaaaactt caag
//
LOCUS       DAOSNN010000050        11763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5418_length_11763_cov_6.483857, whole genome shotgun sequence.
ACCESSION   DAOSNN010000050 DAOSNN010000000
VERSION     DAOSNN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11763
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5418_length_11763_cov_6.483857"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PLQ70_04110"
     CDS             complement(<1..1048)
                     /locus_tag="PLQ70_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HQE33832.1"
                     /translation="MKILLVGEFSRLHNSLKEGLLSLNQKVSIIGSGDDFKEFPVDFS
                     IAPKIISSFSLLKFLNKVFFRVFKSDLQLLEKGIRFYFLLPKLKNYDVVQLINSDAIE
                     TYPFASRYLLKKLFSQNKKISLLICGEETPIIEYLLKKEMKYSILNPLLSDPKLEKSY
                     NYTLKYVQPNYRKTFDFITTRTSSIITSDLDYKLPMEAMGFHSQFIPNPINKDKIEYK
                     QLLINDKIIIFLGINESSAVKKGSVFFEKALEIIKKKYLNQIEIIITRSIPYEQYIEA
                     YNKAHILLDQVYSYDQGYNALEAMAKGKVVFTGAEKEFEEQYNLTEKVAINALPDVDY
                     LVTELSFLIENPEEI"
     gene            1094..1921
                     /locus_tag="PLQ70_04115"
     CDS             1094..1921
                     /locus_tag="PLQ70_04115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQE33833.1"
                     /translation="MTNVFLESDLEILIATKNRNNLDFLVQMFPFTSFSNFNILIINQ
                     SSENQLSSDFEKVRVINVDEKGLSKSRNLAIKNATKKICLIADDDVVYFPNFDTEIIT
                     AFNQNPKASIITFNHQRIGKKEPHNRSKKVYSHSQKTIENVCSIEIGFQLNAIKNNNI
                     CFDENFGLGSYFETAEEYLFLLAAIKLKLNVIFSPFVIVSHPLLSSGEHQGDDKILFA
                     RAALFYKTKRNLAYIWLIKYVFFLFRNNYINWNECMSKYKIGLNGIQKYKELVKLKQ"
     gene            1918..2331
                     /locus_tag="PLQ70_04120"
     CDS             1918..2331
                     /locus_tag="PLQ70_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HQE33834.1"
                     /translation="MTTIDDVQLIEVPKAHDVRGNLSVIEGNTIPFEIKRVYYLYDIP
                     TASVRGGHAHKNLHQLLVALSGSFDVVLKDGNSQKVITLNKPNFGLLIPTGIWRELQN
                     FSSGSVCLVLASAEFNENDYIRNFKEFKLFKNRIS"
     gene            complement(2308..3234)
                     /locus_tag="PLQ70_04125"
     CDS             complement(2308..3234)
                     /locus_tag="PLQ70_04125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HQE33835.1"
                     /translation="MAFFTVIIPLYNKENFIESTLKSVLNQSFGDFEVLIINDGSTDK
                     SEEKVLNFKDPRIHYFYKENGGVSTARNFGIEKASSNYITFIDADDYWYPDFLQEISK
                     NIYRLPDLKVFSAAIEVETSKKVIRSSYSIVKTGDCEMVNYFEASLKETVICTSCAVF
                     HKSVFEKAGGFDIQLKSGEDTDMWIRIGLIFPILFSWKVLARYVYDEKSLSKNHKYPI
                     ECIDFYKYALLEKTNPSLKKFLDLNRFSLAIKSKIIGDKTNFLKFYKAIDLENMSLKK
                     RILLKLPALLLNPLIDLKILLANFGLGNSVFK"
     gene            complement(3235..4122)
                     /locus_tag="PLQ70_04130"
     CDS             complement(3235..4122)
                     /locus_tag="PLQ70_04130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008638929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HQE33836.1"
                     /translation="MLSILIPTYNYNVVPLVLELQKQCLECDINFEILVFDDNSKLCL
                     DENEKINSFENCRFEVLEKNIGRSAIRNLLAKKAKFESLLFLDADTIPIQNHFIKNYI
                     SQVNEDEKIIYGGIEYQKEKPKKNQVLRWKYGNSREALPVKKRQENPYLSFLTLNFLI
                     HKNIFSTVSFNETIPNLRHEDTLFSYNLKQKKIKITHIENPVYHLGLDDFEDAIKKEN
                     ESILCLKYLIDHELLPTDYVRLSKIASKIEKLNLNFAFAFFYRITQSIFLKNIASNNP
                     SLLIFDLYRLVHLCNLNSK"
     gene            complement(4122..4805)
                     /locus_tag="PLQ70_04135"
     CDS             complement(4122..4805)
                     /locus_tag="PLQ70_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQE33837.1"
                     /translation="MSQPVLSLKNVTIVQGGKTILSNINLEVNQGEFIYIIGKTGTGK
                     SSFMKALYADLPLADGEGSIMDFDLATLKEDEIPFLRRKIGFVFQDFQLLPDRNVKDN
                     LHFVLKATGWTDETEIENKINEVLEKVGVKEYVDKMPHQLSGGEQQRVAIARALLNNP
                     DIILADEPTGNLDPQTSAEVLEVLKKINAAGKTVIMATHDYALLMKFPSKTLKCEDAQ
                     IFEVVQKTV"
     gene            4972..7986
                     /locus_tag="PLQ70_04140"
     CDS             4972..7986
                     /locus_tag="PLQ70_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE33838.1"
                     /translation="MQKKNRFIIALFFTGFSILSAQKSAIYTHDSKDFDKALALYNDK
                     QYASAQFIFDKVKSATANEELKSDCSYYVANCAIRTNQADADVLMERFVTDYPTSRKQ
                     NQAYIEVAHYYFNQGSYPQALEWFEKVDENNLSYNEQDQYNFQKGYSFFSSKNKKEAT
                     TYFNKVANSKEYGSQAKYYLGFMAYEGDDYKQATKYFDEVSGEEKYKEKLSYYQADMN
                     FKLGNFQKAIDLGIKAMPKSNAIEQSELNKIIGESYFNLKKYDQSIPYLELYKGKKGK
                     WNNTDFYQLGYAYYMQKNYENAISQFNKIIGGKDFVAQNAYYHLGESYLELNKKQQAL
                     NAFKNASEMDFDKAIQEDASLNYAKLSYEIGNSYQSTPDVLTAFMKKYPNNANQSEIE
                     RLLIDSYISSRNYREAFILLEKNKSPENKLAYQKVTFYRGLELYTDSNYQEALTLFEK
                     SLDIQKDTKFTARATFWKGETEYVLNDFKNAVLSYKQFMNLAGAETTPEFKNSNYNLA
                     YSYFKQKEYDQAGNYFQSYINQSKADKARLNDSYLRLADCRFVTSNYTSALEAYNKVI
                     DSKSVDADYAYFQKAICYGFVTKNDKKIEELNTFLQLYPKSEYRDDAYFELANTYVAQ
                     NKPDLAIKTYDKLNAEFKNGSFTSKAILRQGLIYYNSDKDEQALTKFKKVAADFPKTP
                     EALEAVSTARLIYVDNGKVDEYASWVRTLDFVAVTDAELDNDTYEAAEKQYLQNNTKQ
                     AISGFSGYVGKFPNGIHSLKANFYLAQSYFSDGQETKSIPNYEYVIAQPRNEFTEQSL
                     ARLAQIFLKNDDKSKAIPVLTRLENEADFPQNKIFAQSNLMKSYYENKDYSNSVIYAE
                     KVLANPKMDDNVKSDAQIIIARAAIQTGDEVKARSAYAKLQTIAKGELAAEALYYDAY
                     FKNKDQKFEASNEVVQKLAKNYSSYKYYGAKGLILMAKNFYGLKDSFQATYILESVIK
                     NFTDFPDVVSEAQKELDAIKSEESKTNSSITN"
     gene            8059..9810
                     /locus_tag="PLQ70_04145"
     CDS             8059..9810
                     /locus_tag="PLQ70_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE33839.1"
                     /translation="MKINFKNIIAFALLLTFQFSFAQKKEENIGTEVVNVVKPYTPTI
                     SDAFKVSENPVLDDADNSKKEAIKYSIFSFPVASTFTPSKGKAEGVEKEKQAHLFNNY
                     ATFGIGNYATLNAELFVNQELGNNDYVGGMFRHLSSQGGIKGVELEDAFYDTAIDLTY
                     GVREKDLSWNVDLGYKNQVYNWYGLPTDFGSLLSPVDRNDLINGINPQHSYNNLYLGS
                     RIDINEGVFKEASAKFNHFSDNFGSSENRFYVKPSLQFDINETTIKTNIIVDYVGGNF
                     EKNYSNANSIKYGFTNFGVAPSFAMQQDDWTFNLGVGVFYSADLENNDNKFLVYPQIK
                     ASYKVVGDLMIFYAGAEGNLEQNTYTDFVAMNPYLSPTLNIAPTDKQYDVYAGLKGKL
                     TNTVSYNLKASYVNERDKVLFKSNDYTEISANENYAFGNSMKVVYDDMKTISFYGELK
                     ADISEDLAFGIDGTFSSYTTDIQQEAWNLPSIKLNAKVDFNITEQWYAGANVFFVGER
                     KDQKLNTDIVYVTTPSPVTLDSYFDLNAHLGFKYNERLTAFLKANNIANQNYQKWMNY
                     PVQGFQVVLGANYKFDF"
     gene            10059..10508
                     /locus_tag="PLQ70_04150"
     CDS             10059..10508
                     /locus_tag="PLQ70_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33840.1"
                     /translation="MTFKQKIHQYYQQLVQDREDAFRDMISALTEDSKNDAKGSAGDK
                     HETALSMMHIEQEKLNHKLKEVLAQKAVLDKIDSETIARTIIVGSLVKANGIYLYLSA
                     ALPKIAVDGVNVIALSPQSPLGSHLMGNTTGFTFEINGTKYLIESVE"
     gene            complement(10512..>11763)
                     /locus_tag="PLQ70_04155"
     CDS             complement(10512..>11763)
                     /locus_tag="PLQ70_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33841.1"
                     /translation="KNTLYFLLIITFFSMVFMSCENDDKNYETYNIATPVTMSLSELR
                     SSVKILPPQKTAKSGKIYVYDNFIFINDQENGIHIIDNTIPTAPKKISFLKILGNTDI
                     AVKDQMLFADSFTDLVVFDISDIRNIVEKKRLNNVFPQHIIYPTIDNNLPLLYDYPNY
                     NADKIIVGWSIKKEKRKPQNIDYLSSSGGIMFNAASSESTTGQGGSLARFMIVEDYLY
                     AVDNQKINIFNIQNLSVPTKVKEQNVGWGIETIFNKNDYLYIGSTNGMYIYDIKSLEN
                     PVFASRIAHINACDPVVVDEKYAYVTLRSGNFCGGTNNVLEIIDITNKVNPVKTASFV
                     LENPYGLGIKDQMLFICDGSAGLKVFNKTDVFNLQLTNQFKNINPFDVIPLDDKLLLV
                     GQNTLFQYKYKQNDIELISTFMLE"
BASE COUNT         4059 a         1815 c         1923 g         3966 t
ORIGIN      
        1 ttatttcttc gggattttca attaaaaaag acaattctgt tactaaataa tctacatcag
       61 gcaaggcatt tatcgccact ttctcggtta aattatattg ttcctcaaat tctttttcag
      121 ctcctgtaaa aacaactttt ccttttgcca tcgcttccag agcattgtaa ccctgatcat
      181 aactatacac ttggtctagc aaaatatgtg ctttgttgta agcctcaatg tattgttcgt
      241 aaggaatact tctggtgatg atgatttcga tttggttgag atattttttt ttaattattt
      301 ctagtgcttt ttcaaaaaat acacttcctt tttttacagc actactttcg tttattccta
      361 gaaaaataat gattttatca ttaattagta actgtttata ttctattttg tccttgttta
      421 ttgggtttgg aataaactgc gaatgaaatc ccatagcttc cataggtagt ttataatcca
      481 agtcggaagt gataattgag gatgttcttg tggttataaa atcgaatgtt tttctataat
      541 tgggttgtac atattttaga gtatagttgt aactcttttc taattttgga tctgaaagca
      601 aaggatttaa gatagagtat ttcatttctt tttttaataa atattctata attggcgttt
      661 cttctccaca aattaacagg cttatttttt tgttttgaga aaagagtttt tttagtaaat
      721 atcgagaagc aaaagggtag gtttctatgg catccgaatt gattaattgt acaacatcat
      781 agttttttaa tttgggtaat aaaaaataga aacgaattcc tttttctaat aattgtaaat
      841 cacttttgaa tactctaaaa aatactttat taaggaattt tagtagagaa aaggaagaaa
      901 taatttttgg agctatagaa aaatctacag gaaattcttt gaagtcatct ccgcttccaa
      961 taatactaac tttttgattt agtgacaata agccttcctt taatgaattg tgtaacctgc
     1021 tgaattcgcc aactaaaagt attttcattt tttttgggta gaaatattta tatttacgta
     1081 aatatataag aaaatgacaa atgttttttt agaaagtgat ttagaaattt taattgctac
     1141 aaaaaatcga aacaatcttg attttttagt ccaaatgttt ccttttactt ctttttctaa
     1201 ttttaatatt ttaatcataa atcaatctag tgaaaatcaa ttgtcgtctg attttgaaaa
     1261 agttagggtg attaatgtcg atgaaaaagg attgtctaaa agcagaaatt tagctattaa
     1321 gaatgccact aaaaagattt gtttaattgc agatgacgat gtggtttatt ttcccaactt
     1381 cgatactgaa ataataactg ctttcaatca gaatcccaag gcatctataa ttacatttaa
     1441 tcatcaacgt attggtaaaa aagagcctca taacagatca aaaaaagtat attctcattc
     1501 ccaaaaaact atagaaaatg tatgttcgat tgaaataggt tttcaattaa atgctattaa
     1561 aaataacaat atttgttttg atgagaattt tggattgggt tcttattttg aaacggcaga
     1621 agaatatttg tttttactag ctgctattaa actgaaatta aatgtaatat ttagtccatt
     1681 tgttatcgtt tctcatcctt tattgtcttc tggagaacat caaggagacg ataaaatttt
     1741 atttgcacga gcagctttat tttataaaac aaaaaggaat ttagcttaca tttggttgat
     1801 taaatatgtg ttttttcttt tcagaaataa ttatatcaat tggaatgaat gcatgagtaa
     1861 atataaaata ggcttaaatg gaattcagaa atacaaagaa ttagtaaaat taaagcaatg
     1921 acaactattg atgatgttca actgattgaa gttccaaaag cacacgatgt gagaggaaat
     1981 ctatcggtta tagaaggaaa tacaatacct ttcgaaatta aaagagtgta ttatttgtat
     2041 gatattccaa cggctagtgt tcgtggtggt cacgcacata aaaatctaca tcaactttta
     2101 gtggctttga gtggcagttt tgatgtggtg ttaaaagatg gaaattcaca aaaagtgata
     2161 acactcaata aacccaattt tgggctctta attccaacag gaatttggag agaattgcaa
     2221 aatttttcgt caggttcagt ttgtttggtt cttgcctcgg cagagtttaa tgaaaacgat
     2281 tatattagaa attttaagga attcaaatta tttaaaaaca gaatttccta atccaaaatt
     2341 ggctagtaaa atctttaagt caataagcgg gtttaatagt aaagcgggca acttcaatag
     2401 gattcttttt tttagagaca tattttctaa atcgattgcc ttataaaact ttaaaaaatt
     2461 ggttttgtct cctattattt ttgactttat agccaatgaa aatcgattca aatctagaaa
     2521 ttttttcaaa cttggatttg ttttttctag caaagcatat ttatagaaat ctatgcattc
     2581 tatcggatat ttatgatttt ttgataaact tttttcatca taaacataac gtgctaaaac
     2641 tttccaagaa aacaatattg ggaaaattaa tccaattcta atccacatat cagtgtcttc
     2701 tccgcttttt aattggatat caaagccacc agctttttca aaaacacttt tgtgaaaaac
     2761 agcacacgat gtacaaatga cagtttcttt caggctagcc tcaaaataat ttaccatctc
     2821 acaatctcca gtttttacta ttgaataact tgaacgaata actttttttg aagtttcaac
     2881 ttcaattgct gccgaaaata ctttaaggtc aggtaaacga taaatatttt tagatatttc
     2941 ttgaagaaaa tcaggatacc aataatcatc agcatcaata aaagtgatat aattggatga
     3001 agctttttct ataccaaaat ttctagctgt cgaaacgcct ccgttttctt tgtaaaaata
     3061 gtgtattctt gggtctttaa aattcaatac tttttcttcg cttttgtcag tcgatccatc
     3121 attaataata agcacttcaa aatctccgaa actttgattg agaacacttt ttagtgtgct
     3181 ttcaataaaa ttttctttgt tgtaaagagg tataatgaca gtgaagaaag ccatttattt
     3241 agagtttaaa ttacacaaat gtaccaatcg atacaaatca aaaattaata aagaaggatt
     3301 gttgcttgct atatttttga gaaaaatgga ttgggttatt ctataaaaaa aagcaaaagc
     3361 aaagttgagg tttagttttt caatttttga agctattttt gaaagtcgga cataatccgt
     3421 gggcaataat tcgtgatcaa taagatattt caaacacaaa atcgattcat tttctttctt
     3481 tatggcatct tcaaaatcat ctaaccctaa atgataaacc gggttttcaa tatgtgtaat
     3541 tttaattttt ttttgtttca aattatacga aaacaaagtg tcttcgtgtc ttaaattggg
     3601 aatagtttcg ttgaaagaaa cggttgaaaa aatgttttta tgaattaaaa aatttaaggt
     3661 taagaaagac aagtagggat tctcttggcg ttttttaacc ggtagcgctt ctctcgaatt
     3721 gccatatttc caacgcaaaa cctgattttt ttttggtttt tctttttggt attcgattcc
     3781 tccataaata attttttcat cctcatttac ttgagaaatg taatttttta taaaatgatt
     3841 ctgaattgga atggtatccg catcaaggaa aagtagggat tcgaattttg ctttttttgc
     3901 caataaattt ctaatggcgc tacggccaat atttttttct aatacttcaa aacggcaatt
     3961 ttcaaaacta ttaatcttct cattttcgtc aagacatagc tttgaattgt catcaaaaac
     4021 aaggatttca aaattgatat cgcattccaa acattgcttc tgcaattcca aaaccaaagg
     4081 aactacatta taattatagg ttggaatgag aatagaaagc attaaaccgt tttttgaaca
     4141 acctcaaaaa tttgggcatc ttcacatttc aaagttttcg aaggaaattt cattagcaac
     4201 gcataatcat gagtggccat aataactgtt tttccagcag cattaatttt tttcaaaact
     4261 tcaaggactt ctgcactagt ttgaggatca aggtttccag taggttcatc agctaaaata
     4321 atatcaggat tattaagcaa agcccttgca attgcaacac gttgttgctc gcctcctgag
     4381 agttggtgcg gcattttgtc aacatattct tttactccaa ctttttctaa aacctcgtta
     4441 attttgtttt caatttcggt ttcatctgtc caaccagttg ctttcaatac aaagtgcaaa
     4501 ttgtctttca cattgcgatc gggcaataat tgaaaatctt ggaaaacaaa tcctattttt
     4561 cttctcaaaa acggaatctc atcttctttt aaagtggcca aatcaaaatc cataatgctt
     4621 ccttctccat cggccaatgg taaatcggca taaagtgctt tcataaaact actttttcca
     4681 gttcctgttt ttccaatgat gtatataaat tcaccttgat ttacttcaag attaatattt
     4741 gataaaatgg tttttcctcc ttgaacgatg gtaacatttt ttaaagacaa tacaggttgt
     4801 gacatagtgc aatttatttt atttggtaaa agtaattaat caattaccaa ttatcaatta
     4861 tcaattatca attttttcta aattgttaaa aaacatgttt ataataattc accaaagcct
     4921 ttcgttatag gctacagaat gcaatacatt tgacttctta acaaaaatcc aatgcaaaaa
     4981 aaaaatagat tcataatagc actttttttt acaggttttt caattctttc agcccaaaaa
     5041 tcagctattt atacccacga ttccaaagat ttcgacaaag cacttgcgtt atataatgac
     5101 aaacaatatg cttcggcaca gtttattttt gacaaagtaa agtcggctac tgccaatgaa
     5161 gaactgaaat cggactgttc ttattatgtt gccaattgtg ccattcgtac caatcaagct
     5221 gatgcagatg ttttgatgga gcgtttcgtg accgattatc caacaagtag aaaacaaaat
     5281 caagcttata ttgaagtggc tcattattat ttcaatcaag gaagttatcc acaagctctg
     5341 gaatggttcg aaaaagtgga tgaaaataat ttgagttata acgaacaaga tcaatataat
     5401 ttccaaaaag gctacagttt ttttagttct aaaaacaaaa aagaagccac aacttatttt
     5461 aataaagtgg ctaattcaaa agaatacggt tcgcaagcca agtattatct aggttttatg
     5521 gcttatgaag gagacgatta caagcaagcc accaagtatt ttgacgaagt gtcaggcgaa
     5581 gaaaaataca aagaaaaatt gtcctattat caagccgata tgaatttcaa attaggaaat
     5641 ttccaaaaag caattgattt aggaatcaaa gcaatgccaa aatcgaatgc catcgaacaa
     5701 tcggaattga acaaaatcat tggcgaaagc tattttaatt tgaaaaaata cgaccaatcg
     5761 attccgtatt tagaattgta taaaggcaaa aaaggaaaat ggaacaatac cgatttctac
     5821 caattgggtt atgcttatta tatgcagaag aattacgaaa atgctatttc acaattcaat
     5881 aaaattattg gtggaaaaga tttcgtggct caaaacgcct attatcattt gggcgaaagt
     5941 tatttggaat tgaataaaaa gcaacaagcc ttgaacgctt tcaagaatgc ttccgaaatg
     6001 gattttgata aagcaattca ggaagacgcc agtttgaatt atgccaaatt gagctatgaa
     6061 attggaaatt cttaccaaag cacacccgat gttttgacag cgtttatgaa aaaatatccc
     6121 aataatgcca atcaatccga gatagaaaga ttgttgatag attcgtatat ttcctctaga
     6181 aattatagag aagccttcat tttattagaa aagaataaat cgccagaaaa taaactagct
     6241 taccaaaaag taacttttta tagaggttta gaactttata ccgattccaa ctatcaagaa
     6301 gcactcactt tattcgaaaa atcgttagac atacaaaaag acaccaaatt caccgctcgt
     6361 gctacttttt ggaaagggga aaccgaatat gttttgaacg attttaaaaa tgcggtattg
     6421 agttacaagc aatttatgaa tttggctgga gccgaaacca ctccagaatt caaaaacagt
     6481 aattacaatt tggcttattc ttactttaaa caaaaggaat acgatcaggc tggaaattat
     6541 tttcaatctt atattaatca atcaaaagca gacaaggcac gcttgaatga ttcctacttg
     6601 cgtttggctg attgtcgatt cgtgacttcc aactatacat cggctctcga agcttataat
     6661 aaggtaatcg attcgaaaag tgtcgatgca gattatgcct attttcaaaa ggcaatttgc
     6721 tatggatttg ttaccaaaaa tgataaaaaa atagaggaac tcaatacttt tttgcaattg
     6781 tatcctaaat cagaatatcg tgatgatgcc tattttgaac tcgcaaacac ttatgttgcg
     6841 cagaataaac cagatttggc tataaaaacg tatgacaaat taaatgctga attcaaaaac
     6901 ggttcgttta cttcaaaagc cattttacgt caaggattaa tttattacaa ttctgataaa
     6961 gatgagcaag ccttgacgaa attcaaaaaa gtagcagccg atttccctaa aactcccgaa
     7021 gcactcgaag ccgtttcgac cgctagattg atttatgtag ataacggaaa agtagatgaa
     7081 tatgccagtt gggttcgcac tttggatttt gtagccgtta ccgatgccga attggataat
     7141 gatacttatg aagctgccga aaaacaatat ttgcaaaaca acaccaagca agccatttca
     7201 ggatttagcg gttatgtggg taaatttcca aacggaattc actctttgaa agctaatttt
     7261 tatttggcac aatcttattt ttctgacgga caagaaacga agtcgattcc aaattatgaa
     7321 tatgttattg cacaacctcg aaatgaattt actgaacaat cattggcaag attagcgcag
     7381 attttcttga aaaatgatga taaatcaaaa gctattccag tattaactcg tttggaaaac
     7441 gaagccgatt ttccgcaaaa caaaattttt gctcaatcta atttgatgaa atcctattat
     7501 gaaaataagg attattccaa ttcggttatt tatgccgaaa aagttttagc caatccgaaa
     7561 atggatgaca atgtaaaaag cgatgcacaa attatcattg cccgcgccgc catccaaacg
     7621 ggagatgaag tgaaagctcg ttctgcttac gccaaattgc aaaccatagc caaaggagaa
     7681 ttggctgccg aagccttgta ttacgatgcc tatttcaaaa ataaagatca aaaatttgaa
     7741 gcatcgaatg aagtagttca aaaattagcc aagaattatt cgagttataa atattatgga
     7801 gccaaaggat taatcttgat ggcgaagaat ttttatggat tgaaagacag ctttcaggcg
     7861 acttatattt tggaaagtgt gataaaaaac ttcacagatt tccctgatgt agtttcagaa
     7921 gctcaaaaag aattggatgc catcaaaagt gaagaatcca aaacgaattc gtcgataact
     7981 aattaaatat aatatccgtt gaaatccgag tctttgcttt agcaaatttg cgtttatctg
     8041 cgttccaaat atcaaaatat gaaaatcaat ttcaaaaata taattgcctt tgctttatta
     8101 ctaacttttc aattttcttt cgcccaaaaa aaggaagaaa acatcggaac agaagtcgta
     8161 aatgttgtta agccttatac gccaaccatt tcggatgcct ttaaagtaag cgaaaatcct
     8221 gttcttgacg atgcagataa ttccaaaaaa gaggccatca aatattcgat tttttctttt
     8281 cctgtcgcat ctacttttac gccatctaaa ggaaaagcag aaggagtcga aaaagaaaag
     8341 caagctcatt tgtttaataa ttacgccact tttggaatag gaaattatgc tactttgaat
     8401 gcggaattat ttgtgaatca agaattagga aataatgact atgtgggtgg aatgtttcgt
     8461 catttgtcct cgcaaggcgg aattaaaggc gtcgaattgg aagatgcgtt ttatgatact
     8521 gccattgatt tgacttatgg agttcgcgaa aaagatttgt cgtggaatgt agatttgggt
     8581 tataaaaatc aagtttacaa ctggtatgga ttgccaacag attttggcag tttattatcg
     8641 ccagtagata gaaatgattt aatcaatgga attaatccgc aacacagtta taacaatctg
     8701 tatttaggaa gtagaattga tataaatgaa ggtgttttta aagaagcgag cgccaaattc
     8761 aatcattttt cggataattt tggttcttct gaaaatagat tttatgtaaa accttctttg
     8821 caatttgata tcaacgaaac taccataaaa accaatataa ttgttgatta tgttggagga
     8881 aatttcgaaa aaaattattc taacgccaac tcaataaaat atggttttac caatttcgga
     8941 gtcgcgccaa gtttcgctat gcagcaagac gattggacgt ttaatttggg cgttggagtt
     9001 ttttatagtg ccgatttaga aaacaacgac aataagtttc tagtttatcc acaaatcaaa
     9061 gcttcctata aagtagttgg tgatttgatg attttctatg ctggagccga aggaaatttg
     9121 gagcaaaata cttataccga ttttgtagct atgaatcctt atttgtctcc aaccttaaat
     9181 attgctccaa ccgacaaaca atatgatgtt tatgcgggtt tgaagggaaa gttgaccaat
     9241 acggtgagct acaatcttaa agcttcatat gtgaacgaaa gagataaagt tttgttcaaa
     9301 agcaatgatt atacagaaat aagtgcgaat gaaaattatg ctttcggaaa ttctatgaaa
     9361 gtggtttatg atgatatgaa aaccattagt ttttatggtg aattgaaagc cgatatttct
     9421 gaagaccttg cttttggtat tgacggaact tttagtagtt ataccacaga tattcagcaa
     9481 gaagcttgga atttgccatc cataaaacta aatgcaaaag ttgatttcaa cataaccgaa
     9541 caatggtacg ctggagcgaa tgtgtttttt gttggcgaac gaaaagacca aaaattaaat
     9601 acagatatcg tgtatgttac aacaccaagt ccggtaactt tggatagcta ttttgatcta
     9661 aatgcacacc taggttttaa atacaatgaa agattaactg cttttttaaa ggccaataat
     9721 attgccaatc aaaactatca aaaatggatg aattatccag ttcaaggatt tcaagtagtt
     9781 ttgggagcga attataaatt tgatttttag attatgataa agcttctggt tattactgtc
     9841 tttatcttat ttgtctttgt ttttatttat tttggttatt actaagatta taagaggaat
     9901 ccaagagatt ttacggggtc aattattgga atgccaatag gtatgatact agagacattt
     9961 ggttttaaaa ctttaaacga gaaattaaaa aaatgggtta atgaagattc taaaaaataa
    10021 aaatctgcgg aaatccgttt catccgcgtt ccaatattat gactttcaaa caaaaaatac
    10081 accaatatta ccagcaactt gttcaagacc gtgaagatgc tttccgtgat atgatttcgg
    10141 ctttgaccga agattcaaaa aacgatgcca aaggttcagc aggcgacaaa cacgaaacgg
    10201 cattgtcgat gatgcacatc gagcaagaaa aactcaatca taaattgaaa gaagtcttgg
    10261 ctcaaaaagc cgttttagac aaaatcgatt cagaaaccat tgctagaacg ataattgttg
    10321 gcagtttggt caaagccaat ggaatttatc tttatttaag tgccgcactt ccaaaaattg
    10381 ctgttgatgg agtgaatgta attgcgcttt ctccacaatc accgttaggg tctcatctaa
    10441 tgggcaatac aacaggattc acttttgaaa ttaatggaac gaagtatttg attgagagtg
    10501 tggaatgatt tttattccaa cataaaagtg cttatgagtt caatgtcgtt ttgtttgtat
    10561 ttgtattgaa aaagtgtgtt ttgaccaacc aaaagtagtt tgtcatccaa aggaatgaca
    10621 tcaaacggat taatgttttt aaattgattg gtcaattgca aattaaagac atcggttttg
    10681 ttgaatactt ttagtccagc acttccatca caaataaata acatttgatc ttttattccc
    10741 aaaccataag gattttctaa aacaaacgat gctgttttta ccggattcac tttattagtg
    10801 atgtcaataa tttctaagac attattggtt cctccgcaaa aattacctga acgcaacgta
    10861 acgtaggcgt atttttcgtc aactactaca ggatcgcaag cattgatatg ggcaattctt
    10921 gatgcgaaaa caggattttc taacgatttg atgtcataaa tatacatacc gttggtgctc
    10981 cctatgtaca aatagtcatt tttgttaaaa atggtttcaa ttccccaacc cacattttgc
    11041 tcttttactt ttgttggaac agacaaattt tgaatgttga aaatattgat tttctgatta
    11101 tccacagcat acaaataatc ttcaacaatc ataaaacgag ccaatgaacc gccttgtcct
    11161 gtagttgatt cagaacttgc tgcattgaac ataattccac ctgaactgct taaataatct
    11221 atgttttgag gttttctttt ttcttttttg atactccaac ctacaatgat tttgtctgca
    11281 ttataatttg gataatcata caaaagtgga agattattgt caatagttgg gtaaatgata
    11341 tgttgtggaa aaacattgtt caatctcttc ttctctacta tatttctaat atctgaaata
    11401 tcaaaaacga ctaaatctgt aaaactatcg gcgaaaagca tttgatcttt aaccgctata
    11461 tctgtatttc caagaatttt aagaaaggat attttttttg gagccgttgg aattgtattg
    11521 tctataatgt gaatgccatt ttcttggtca tttataaaaa taaaattatc gtaaacatat
    11581 attttaccag attttgcggt tttttgtgga ggtaatattt ttacagaact tcttaactcg
    11641 ctcaaagaca tagtgaccgg agttgcaata ttgtaggttt cataattttt atcatcattc
    11701 tcgcaagaca taaaaaccat agaaaagaaa gttattatta gcaaaaaata taaagtattt
    11761 ttc
//
LOCUS       DAOSNN010000051        11695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5470_length_11695_cov_5.722509, whole genome shotgun sequence.
ACCESSION   DAOSNN010000051 DAOSNN010000000
VERSION     DAOSNN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11695
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5470_length_11695_cov_5.722509"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..871)
                     /locus_tag="PLQ70_04160"
     CDS             complement(56..871)
                     /locus_tag="PLQ70_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33842.1"
                     /translation="MKTRILFLMLFVASYSFSQTVNDYKAVVIPMKYDFQKEENQYRL
                     QTITKMNLQKAGFQAFYSNEPILGQFPDRCSLLYVDVLKESGFLTTKLYVVFKDCFGL
                     VIFKSALGTSREKEYGPAYSESLNLAFGTVYELKYQYNGNTNFGPKSSIAPQSMPIPL
                     ASTAAVPAVAVPVVPTAAVVAVPAVSTSNNVAKAAESKSVGLLYAQPTSYGYQLIDSE
                     PKVVMKVYKTSNPNSYMAKKGDVQGTLFSKDNQWFFEYYQGDNLVSEKIEVKF"
     gene            complement(876..1571)
                     /locus_tag="PLQ70_04165"
     CDS             complement(876..1571)
                     /locus_tag="PLQ70_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HQE33843.1"
                     /translation="MKITNSNITDVFFDLDHTLWDFEKNSALAFETVFKMQDLAINMT
                     EFLQFYVPINREYWERYRKDEISQKQLRFGRLKDTFDLMRFEIEDDLIVTLSEQYIHY
                     LPKFNHLFDGTIEILDYLKAKYSLHIITNGFAEIQENKLNNSYITHYFKTITNSEMAG
                     VKKPNSLIFDYALNLAKAKKENSIMIGDCIEADVQGALDAGLDAVFFNENNVSVPQTI
                     KQVNHLLELKKYL"
     gene            complement(1575..2267)
                     /gene="radC"
                     /locus_tag="PLQ70_04170"
     CDS             complement(1575..2267)
                     /gene="radC"
                     /locus_tag="PLQ70_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQE33844.1"
                     /translation="MEQTNFPITHWSEDDKPREKLMLKGKSALSDAELIAILIGSGNR
                     NESAVGLSKRILASVDNNLNALGKLTIQQLMSFKGIGEAKAISIMAAMELGRRRRAEE
                     AVELKKITSSKMIFELMQPIIGELPHEEFWVLYLNNSNKVLSKSQQGKGGITGTVADT
                     RLVFKVALELGATAIILCHNHPSGNLKPSEADKKLTQKLKAAGQTLDIPVLDHVIITE
                     NGYYSFVDEDIL"
     gene            complement(2272..3627)
                     /locus_tag="PLQ70_04175"
     CDS             complement(2272..3627)
                     /locus_tag="PLQ70_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQE33845.1"
                     /translation="MRTHFIAIGGSAMHNLALALHNKGYQVTGSDDAIFEPSKSRLAK
                     KGILPAELGWFPEKITQDIEAVILGMHAKADNPELLKAQELGLKIYSYPEFLYEQSKN
                     KTRVVIGGSHGKTTITSMILYVMHYHNIEVDYMVGAQLEGFDTMVHLTEENDFMVLEG
                     DEYLSSPIDRRPKFHLYQPNIALISGIAWDHINVFPTYENYVNQFEIFIQKITNGGIL
                     VYNEEDSEVKRVAEAATNPIRKMPYTTPKYEVKDGVTLLETPEGAMPIEVFGAHNLNN
                     LAGAKWICQNMGVDEADFYEAIASFKGASKRLEKIAEGKGKVAYKDFAHSPSKVAATT
                     KAVKEQYPNRTLVACLELHTYSSLNAEFLKEYEGALEYSDVAVVFYSPDAVKIKQLEE
                     VTYEQIAKAFNREDLIIYTNPAEFKTFLFNQNLENSALLLMSSGNYGGLNFDEVKTLI
                     E"
     gene            complement(3679..4368)
                     /locus_tag="PLQ70_04180"
     CDS             complement(3679..4368)
                     /locus_tag="PLQ70_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE33846.1"
                     /translation="MKKLISFFLLFPLLIWSQSDYSKAEKLFDAGKYEQAQPVFESFL
                     KQNPSHLKTIEYLGDIAGHEKSWDKAIGYYKKLKQLKPSEANYYFKYGGVLGMKAKES
                     NKFKALGMIGEVKSSFEKAIELNPKHIEARWALVMLYLQLPGIVGGSETKAIKYSNEL
                     MKLSPVDGYLSRGQIDEYFERYTAAEKNYIKANEIGKSKVTFQVLYNLYLKKLKDPKK
                     AADLKQQFDKK"
     gene            4497..11030
                     /locus_tag="PLQ70_04185"
     CDS             4497..11030
                     /locus_tag="PLQ70_04185"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQE33847.1"
                     /translation="MKNILFAFLLISTSFYAQNYEKNWNKVIKNENNGKIKSANAIVA
                     KIHKKAVADKNEVQIIKCFFYESKYLQVVDENAQTKIINNLKAEINRASIPSKAILNL
                     VYAKCLSDYYDDNDYIVRSRTNTVSLDDDFLTWTNTDFINQIDTSFQNTLKDETILKS
                     TSLSQYEELFDFFTLEKFNKQNLFDYLLGENIDYYTQKIEQWGINKSKFIVFEKELIG
                     KSADFIQLNFDFTKDENLKKALVLYQKQEMNASTLENQFDRMQFCDTYLLNSNDTFMK
                     SLNLLQKESKDIILTQKIQLEKAVVLNKQASKETHPDYKIQAIAILDSIVKINNRSNA
                     HKQALQKKQDIISKSLNIQLQKYCYTDENTRAFIQYKNLNRLSVSFYKIDLKTLKYFR
                     NSPYNKDSLASTIIKNKKTIASKNYELEDKKNYFEYTTEVLLPQLKTGSYLVYFESDS
                     DAKDKKAFAYETITISNIAVLASQKDKTENYQVLDRKTGKPLENVSIELLNQTLKTDK
                     NGLATCIKENNNYNYGPIQFTTTTDSLLINNHYLYNNYSYANIVNNKPTGKVEFYLDR
                     AIYRPGQTVYYKGVAIQKNKDKTNLTTKTTFKITIQDPNNTVFKEFEVTTNDFGSFSG
                     EFVLPKTGLTGNFRMMVKKPESPLKTNFNFIEQNQSSFWDKVDFNISGTTFEVEEYKR
                     PKFEVSFEPVKKEYQVNQNVSVTGLAKAFAGSNISDAKVEYKVRLTPYTLNNGYYYNL
                     NGKSESILVGETKTDASGKFEINFLAKPFENATKEQLPIFEYSITASVTDINGETHKT
                     YTSVNIGYHSLKLSTTIPKRLETKDKNDIILNSTNLNGEFLATKGELKLYYTSPFSNK
                     FKARVWQKPEIETISEQDFTRLFPYEQNEKPVSDKELGTLVYSKKIDTEKDKKLVLNF
                     ISNYKSGNYKVVFSAKDSFDNSIETSSNFQIIQSKDKFNPSKLFTAIQINKDAKKDGF
                     VLVKLTSIIPELYITTTGNYQSYLYFEDTFHLQNNETIIKIPLKKEFEKRMSIGFESV
                     FENQTFKDHIEVVLKVEEPKLEFIVESFRNKIQPGNNENWSFQLKSSNTSKEAEVLAS
                     MYDSSLDQFAKRDWNGLTFGDNYNNSSNFKTGLGFDKISSPIRNLNSIQNRIELNDEN
                     TKLMWFGFDFNNSFTPQQLRDYQKQLNNKSKKPKNAKMISGIVSDKSGPLPSVSVVIK
                     GIQRSTQTDFDGYYQIEAAIGEELAFSLIGFETKKITISTKVIDVQLEESSNTLEEVV
                     VVGYGAVKKKGFTGSVAAVIEEDNTIYNTAGIDIRLEDVMQGRVAGVNITVNGKSENN
                     SNIRIRGANSISGSEPLYIVDGVTIPEEQFKLISPTDIISIDVLKDAAATAIYGAKAA
                     NGVIIITTKKALEALTQVKARKNLSEMAFFYPNLKTDAKGKLSFNFTSPEALTAWKLR
                     LMAHNKDAVTGYLEKSVITQKELMLTPNFPRFFREKDSIVITAKVANITNDVKTGIAV
                     LQLFDATTMQSIDSKMNNTNTIKNFTIPAYGNTTVSWKIYIPEGLQGVQYKVLAKAGN
                     FSDGEENILPVLTNNMLVTESIPIWVRENSKKEYTFENLKNNTSTSLRNHQFTLEYTS
                     NPTWLAIQSLPYLMEYEHECAEQTFARFYANALASEIISSNPKIASLFETWRKNGKLN
                     SKLEENEELKSMILAETPWLNDAQNEDEKKKNLALLFDLEKMKSSQEATFDKLKQKQK
                     PSGGFAWFDGSEESEYITRHILAGLGHLSKLTKDENNATKIKEIATTGVPFMDAKFLE
                     NYKNTTKNWKKTDKLIWFNPYSDLHYLYTRGFYLENYPLSDTLKKATKLYLEAAKKDW
                     LTYSLYEKGLAALTLHRFGEKDAAKKIIESLKETASNNEDWGMYWIANKAGWYWYQAP
                     IETQALLIEAFAEVSNDTKSVDAMKVWLLKNKQTKNWPTTKSTTEAIYALLLQGTNWL
                     SVKDNTIIKIGDQKILTKKLSENEKEAETGYVKLNWKADEIKKEMATIVVENKSKVPG
                     FGGVYWQYFEDLDKIKTNSGAVLSVSKELYLKKSTLKGEELQKITTKNSLKIGDLVTV
                     RLVITSKEDMEFVHLKDMRASCFEPVDVLSSFEYKAGLGFYKSTKDAATHFFFDTINK
                     GTYVLEYDIRVNNLGDFSNGITTIESMYAPEFSSHTKGIRVNVKE"
     gene            11157..>11695
                     /locus_tag="PLQ70_04190"
     CDS             11157..>11695
                     /locus_tag="PLQ70_04190"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQE33848.1"
                     /translation="MKRKRKLPLVVISDIHLGTYGCHAKELLQYLKSIHPQTLVLNGD
                     IIDMWNFSKSYFPAAHMEVLRYIIKMSNLGTRVIYITGNHDEALRKYSDFILGNLELV
                     DKLILDLDGKKTWIFHGDVFDSSTKGYAKILAKLGGKGYDLLILINSFINWVLVACGR
                     EKRSFSKTIKNSVKKAVSFV"
BASE COUNT         4069 a         2158 c         1922 g         3546 t
ORIGIN      
        1 tctgaaatct gaaatctgaa atctgaaatc tgaaatctga aatctgaaat ctgaactaaa
       61 acttaacctc tattttttca gaaactaagt tatctccttg ataatattca aaaaaccatt
      121 gattgtcttt tgaaaacaaa gtcccttgaa catccccttt tttggccata taactatttg
      181 gattagatgt tttatacact ttcatcacta cttttggttc gctatcaatc aactgataac
      241 cgtaagatgt aggttgtgca tataacaaac ctacactttt actttcagct gctttagcta
      301 cgttatttga cgttgaaaca gctggaactg caacaacagc agcggtcgga actactggaa
      361 ccgcaacggc tggaactgca gctgttgacg caagtggaat tggcattgat tgtggagcaa
      421 tactcgattt gggaccaaaa ttagtattgc cattgtattg atatttcaat tcataaacag
      481 ttccgaaagc taaattcaat gactcagaat aagcaggacc atattctttt tccctactcg
      541 ttccgagagc cgatttaaaa ataacaagtc caaaacaatc tttaaaaaca acatacaatt
      601 ttgttgtcaa gaaaccactt tctttcaata catcaacata taatagactg catcgatctg
      661 gaaactgacc aagaattggc tcgttcgaat aaaaagcttg aaaaccagct ttctgtaaat
      721 tcattttagt aattgtttga agacgatatt gattttcttc tttttgaaag tcgtatttca
      781 ttggaattac aaccgcttta taatcattaa ccgtttgcga aaagctataa ctcgcaacaa
      841 acaacatcag gaataggatt ctagttttca taattttata aatatttttt taattctaat
      901 aaatggttta cttgtttaat tgtttggggt actgaaacat tgttttcatt aaaaaaaaca
      961 gcgtccaaac ctgcatccaa agcgccttga acatcagctt caatgcaatc gccaatcata
     1021 atgctattct cttttttagc tttagctaaa ttcaaagcat agtcaaaaat aagagaatta
     1081 ggctttttta cccccgccat ttccgaattg gtaatggtct taaaatagtg cgtaatatac
     1141 gaattattca atttgttttc ttggatttct gcaaaaccat tggtaatgat gtgcaagcta
     1201 tatttggcct tcaaataatc caaaatttca atcgttccgt caaacaaatg attgaatttg
     1261 ggtaaataat gaatgtattg ttcggataaa gttacgatca aatcgtcttc aatttcaaaa
     1321 cgcatcaaat cgaaagtgtc tttcaatctg ccaaaacgaa gttgcttttg tgaaatttcg
     1381 tctttacgat atcgctccca atattcacga ttgataggca cataaaattg gagaaattca
     1441 gtcatattaa ttgccaaatc ttgcattttg aaaaccgttt caaaggctag agccgaattc
     1501 ttttcaaaat cccaaagcgt gtgatccaga tcgaaaaaga catctgttat gttgctgttt
     1561 gttattttca tttcttataa aatatcttca tcgacaaaac tgtaataccc attttcagta
     1621 ataattacgt gatccaaaac aggaatatcc aacgtttgtc cagctgcttt gagtttctgt
     1681 gtcaattttt tatctgcttc actaggtttc aaatttcccg aaggatgatt gtgacataga
     1741 attattgccg tcgctcccaa ttctaaagca actttaaaaa ccaatcgagt atcggcaacc
     1801 gttcctgtaa taccgccttt gccttgttgc gatttcgaaa gtactttgtt cgaattgttc
     1861 aaatacaaaa cccaaaattc ctcgtggggt aattccccaa taatgggttg catcaattcg
     1921 aaaatcattt tgctggaggt aattttcttc aattcgacag cctcttctgc ccttcttcgt
     1981 cttcccaatt ccattgctgc cataatcgaa atggctttgg cctcgcctat tcccttgaag
     2041 ctcatcaatt gttggatggt taattttccc aaggcattca aattgttgtc tacactcgcc
     2101 aaaattcgtt tactcaatcc caccgccgat tcgtttcggt ttcccgaacc aatcaaaatg
     2161 gcaatcaatt cggcatcgct caacgcactt ttacctttca gcatcagctt ttcgcgtggt
     2221 ttgtcgtctt cggaccagtg cgttataggg aaattagttt gttccatttg attactctat
     2281 caacgtcttt acctcatcaa aatttaatcc gccataattt ccagaactca tcaacaacaa
     2341 tgcagaattt tctaaatttt gattaaataa aaaggtttta aattcggctg gattggtata
     2401 aataatcaaa tcttctctgt tgaaggcttt ggcaatttgc tcataagtta cttcttcgag
     2461 ttgcttaatt tttacagcat caggcgaata gaaaaccaca gccacatcgg aatattccaa
     2521 tgcgccttca tactctttta agaattctgc attcaaactg ctgtaggtgt gcaattccaa
     2581 acaagctacc aaagttcggt ttgggtattg ttctttcacg gctttggtcg tagccgctac
     2641 tttgcttggc gaatgggcaa aatctttgta agctactttt ccttttcctt cagcaatttt
     2701 ttccaaacgt ttggaagctc ctttgaaact cgcaatggct tcataaaaat cggcttcgtc
     2761 cacgcccata ttttggcaaa tccatttggc tccagccaag ttgttcaaat tatgcgcacc
     2821 aaaaacctct attggcattg ctccttctgg agtttctagc agtgtaactc cgtctttcac
     2881 ttcatatttt ggagtcgtgt aaggcatttt tcgaattgga ttcgtagctg cttcggcaac
     2941 acgttttact tctgaatctt cttcgttgta aaccaaaatt ccaccattgg ttattttttg
     3001 gatgaaaatc tcgaactggt ttacgtagtt ttcgtaagtt ggaaaaacat tgatatgatc
     3061 ccaagcaatt cctgaaatca aggcaatatt cggttggtac aaatgaaatt ttggtcttcg
     3121 gtcgattgga gatgacaaat attcgtcacc ttcgagaacc ataaaatcat tctcctcggt
     3181 caaatgcacc atcgtgtcaa aaccttcgag ttgagctccc accatataat cgacttcgat
     3241 attgtggtag tgcatcacgt acaaaatcat cgaagtgatg gttgtttttc cgtgcgaacc
     3301 accaataacc acgcgggttt tgtttttgga ttgttcgtat aaaaattcgg gataggaata
     3361 aattttcagt cccaattctt gtgctttcaa taattcggga ttgtcagctt tggcgtgcat
     3421 tccgagaatt acggcttcga tatcttgggt gattttttcg ggaaaccaac ccagttctgc
     3481 tggcaaaatt ccttttttgg ccaaacggga tttcgatggt tcaaaaatgg catcgtcact
     3541 tcccgtaact tgatatcctt tgttgtgtaa tgccaaagcc aaattgtgca ttgcgctgcc
     3601 gccaatggcg ataaaatgag ttctcatact aatatttttc caattattaa attccaatta
     3661 ttcgaaattg gatgtagatt attttttgtc gaattgctgt tttaaatcgg ccgccttctt
     3721 ggggtctttt aatttcttca aatataaatt atataaaact tgaaacgtaa cttttgattt
     3781 accaatttca ttggctttga tataattttt ttcggcagcg gtatatcttt caaaatattc
     3841 gtcgatttga cctcgggaca aataaccatc cacaggagat aatttcatca actcattcga
     3901 atatttaatt gcttttgtct cgcttccgcc cacaattccg ggcaattgga ggtataacat
     3961 taccaatgcc caacgtgctt cgatgtgttt tggattcaat tctatggctt tttcgaagga
     4021 ggatttgact tcgccaatca ttccgagtgc cttgaacttg ttggactctt tggctttcat
     4081 tcccagtacg ccgccgtatt taaaataata attggcttcg gaaggtttta attgttttag
     4141 ttttttatag tagccaatgg ctttgtccca agatttttcg tgacccgcaa tgtctcccaa
     4201 atattctatc gtttttagat gagagggatt ttgttttaaa aaactttcga aaacgggttg
     4261 cgcttgctca tattttccgg cgtcgaataa tttttcggcc ttgctgtaat cggattgcga
     4321 ccaaattaat agcggaaata gtaaaaagaa agagatcagt tttttcattt ttcaaaaata
     4381 agaaaataca ggaattttcg ttagctttta agtaaaaatt agtactttta aaaaataatt
     4441 gacaagcatt ccaacctttt tcaaataatt gccctctact tatataaaac caaaatatga
     4501 aaaatatctt atttgccttc cttctaattt caacttcttt ttatgctcaa aattatgaga
     4561 aaaactggaa taaagtaatc aaaaatgaaa ataacggcaa aattaaatcg gcgaatgcca
     4621 tcgttgccaa aatccacaaa aaagcggttg ccgacaaaaa cgaagtgcaa atcatcaaat
     4681 gttttttcta tgaatcgaaa tatttgcagg tggttgacga aaatgcgcaa accaaaatca
     4741 tcaacaattt aaaagccgaa atcaatcgag cttcgattcc ttcaaaagcg attttgaatt
     4801 tggtttatgc gaagtgtttg agtgattatt atgatgacaa cgattatatt gttcgaagca
     4861 gaaccaatac tgtaagcctc gatgatgatt ttttaacttg gacaaatact gattttatca
     4921 atcaaattga cacttcattt caaaatacat taaaagatga aaccattcta aaatcgactt
     4981 cattaagtca atacgaagaa ttatttgatt tttttacttt agaaaaattc aataaacaaa
     5041 acttgtttga ttatttatta ggtgaaaaca ttgattatta cacccaaaaa attgaacaat
     5101 ggggaattaa taaaagtaaa tttattgtct ttgaaaagga actcatagga aaatcagcag
     5161 attttatcca attgaatttc gattttacaa aagacgaaaa tctaaaaaaa gcacttgttt
     5221 tgtatcaaaa gcaggaaatg aatgcttcta ctttagaaaa ccaattcgat agaatgcaat
     5281 tttgcgatac ttatttgcta aattccaacg atacttttat gaaatctttg aatcttcttc
     5341 aaaaagaatc taaagacatt atcttaactc aaaaaattca attagaaaaa gcagttgtcc
     5401 tgaacaaaca agcatcaaaa gaaacacatc ctgattacaa gattcaagct attgcaatac
     5461 ttgacagcat tgtaaaaatc aataatcgtt ctaacgcaca caaacaggca ttacaaaaga
     5521 aacaagatat tatttcgaaa tcgctcaaca ttcagctaca aaaatactgt tataccgacg
     5581 aaaacaccag agcttttatt caatataaaa acttaaatcg tctatcggtt tctttttata
     5641 aaatcgacct taaaacctta aaatacttcc gaaattcccc atacaacaaa gacagtttag
     5701 ctagtacaat tattaaaaat aaaaaaacaa ttgcttctaa aaattatgaa ttggaagata
     5761 aaaaaaacta ctttgaatac actaccgaag tcctcttacc acaattaaaa actggaagct
     5821 atttagttta ttttgaaagc gattctgacg caaaagacaa aaaagcattt gcttatgaaa
     5881 ctattaccat ttcgaatatt gctgttttgg cttctcaaaa agacaaaacc gaaaactacc
     5941 aagttttgga cagaaaaacc ggaaaacctt tagagaacgt ttctatagaa ttactaaatc
     6001 aaacgctcaa aacagataaa aatggtttag caacttgtat caaagaaaac aacaactaca
     6061 attatggccc tatccaattt acaactacaa ctgattcgct tttaataaac aatcactatt
     6121 tatataataa ctatagttac gcaaacatag taaacaacaa acctactgga aaagtcgaat
     6181 tttatttaga tcgcgccatt tatcgtccag ggcaaactgt ttactataaa ggagttgcta
     6241 ttcagaaaaa caaggacaaa acgaatctga ctaccaaaac tactttcaaa attacaattc
     6301 aagacccgaa taatactgtc tttaaagaat ttgaagtcac aaccaatgat tttggctctt
     6361 tttcgggaga atttgttctg ccaaaaactg gattaactgg caatttcaga atgatggtaa
     6421 aaaaacctga aagtcccttg aaaaccaatt tcaattttat cgaacaaaat caatcaagct
     6481 tttgggataa agtagatttt aatatttctg gaacaacgtt tgaagtcgaa gaatacaaac
     6541 gtccaaaatt tgaagtaagt tttgaacctg taaaaaaaga gtatcaagtc aatcaaaatg
     6601 taagcgtgac tggacttgcc aaagcttttg caggaagcaa tatttcggat gcaaaagtag
     6661 aatacaaagt tagacttaca ccttatacct taaacaacgg atattactac aacctaaacg
     6721 gtaaatctga atccatttta gttggcgaaa ctaaaacgga tgcttcggga aaatttgaaa
     6781 taaatttcct tgccaaacct ttcgaaaatg caacaaaaga acagctaccc atatttgaat
     6841 acagcattac agcctcagtc accgacatca atggtgaaac gcacaaaacc tatacatctg
     6901 ttaatattgg ttatcattct ttgaaacttt ctacaaccat tccgaagcga cttgaaacta
     6961 aggataaaaa cgatattatc ctaaacagta ctaatctaaa cggagaattt ttagccacaa
     7021 aaggcgaact aaaactctat tatacaagtc ctttttcaaa taaattcaaa gcaagagttt
     7081 ggcaaaaacc cgaaatagaa accatttccg aacaagattt tacaagatta tttccttatg
     7141 aacagaatga aaaacctgtt tccgacaaag agctaggcac tttagtttat tctaaaaaaa
     7201 tagacactga aaaggataaa aaattggttt tgaattttat ttctaattac aaatcgggaa
     7261 actataaagt agtattttca gccaaagata gttttgataa ttctatagaa actagttcta
     7321 attttcaaat aatccaaagc aaagacaaat tcaatccgag taaattattc actgcaatac
     7381 aaattaataa agatgctaaa aaagatggtt ttgttttagt aaaattgact tcgattattc
     7441 cagaactcta tattacgaca actggaaatt atcaaagtta cctttatttt gaggatactt
     7501 tccacttgca aaacaatgaa actattatca aaattccgct gaaaaaggaa tttgaaaaaa
     7561 gaatgagcat aggttttgaa agtgtttttg aaaatcaaac ttttaaagat cacatagaag
     7621 ttgttcttaa agtcgaagaa ccaaaattgg aatttattgt agaaagtttt agaaacaaaa
     7681 tacaacccgg aaacaatgaa aattggtcgt ttcaattaaa atcatctaat acctcaaaag
     7741 aagccgaagt tttagcctcg atgtacgata gctcgttaga tcaatttgcc aagagagatt
     7801 ggaatggttt aacctttggc gataactata ataattcttc caactttaaa acaggtttag
     7861 gttttgacaa aatatcgagt cccatcagaa acctgaattc tattcaaaat cggattgaac
     7921 ttaatgatga gaacaccaaa ttaatgtggt ttggattcga cttcaacaac tcattcacac
     7981 cacagcaact tagagattat caaaaacaat taaacaacaa aagcaaaaaa cccaaaaatg
     8041 caaaaatgat atcaggaatt gtctcggata aaagtggtcc tttacccagc gtaagcgttg
     8101 ttataaaagg aattcaacga agtacacaaa ctgactttga tggttattat caaatagaag
     8161 ccgccattgg cgaagaatta gcattctctt taattggatt cgaaactaaa aaaattacaa
     8221 tatccaccaa agtaattgat gtccaattag aagaatctag caatacttta gaagaagtag
     8281 ttgttgtggg ttatggagcc gtcaaaaaga aaggattcac tggatctgtt gcagctgtga
     8341 ttgaagaaga caatactata tataatacag caggaataga tataagatta gaagatgtaa
     8401 tgcagggaag agttgcagga gttaacataa ctgttaatgg gaaatctgaa aataattcaa
     8461 atattagaat tcgtggagct aattctattt caggaagcga gcctctttat attgttgatg
     8521 gcgtaacaat acccgaagaa caattcaaat taatttctcc aaccgatatt atttccatcg
     8581 atgttctaaa agatgccgca gcaacagcaa tttatggtgc taaagccgca aacggggtca
     8641 ttatcatcac gactaaaaaa gcccttgaag ccttaaccca agtaaaagct agaaaaaatc
     8701 tatccgaaat ggctttcttt tatccgaatt taaaaacaga tgcgaaagga aaattgagtt
     8761 tcaatttcac ttcgcctgaa gctttgaccg cttggaaact acgtttaatg gcacacaaca
     8821 aagatgctgt tactggttat ttggaaaaaa gtgtcattac ccaaaaagag ttgatgctta
     8881 caccaaactt cccaagattt ttcagggaaa aagacagcat cgttatcacc gcaaaagtgg
     8941 caaatattac caatgacgtc aaaacaggaa tcgctgtttt gcaactattc gatgccacca
     9001 caatgcaaag tatcgatagc aaaatgaaca atacaaatac cattaaaaac ttcacgattc
     9061 ctgcttacgg caacaccacc gtgagttgga aaatctacat tcccgaagga ttgcaaggcg
     9121 ttcagtataa agttttggcc aaagccggca atttctccga tggagaagaa aacatccttc
     9181 ccgttttgac caataatatg ctcgtgaccg aaagtattcc gatttgggtt cgagaaaact
     9241 ccaagaaaga atacactttc gagaacctga aaaacaacac ttcaacctct ttacgaaatc
     9301 atcaatttac gttggagtac acttcgaatc caacttggtt agccattcaa tctctgccgt
     9361 atttgatgga atacgaacac gaatgtgccg aacaaacttt tgctcgtttt tatgccaatg
     9421 ctttggcttc ggaaattatt tccagtaatc cgaaaatcgc cagtcttttc gaaacttgga
     9481 gaaaaaacgg aaaactcaat tcaaaactcg aagaaaacga agaactaaaa tcgatgattc
     9541 ttgccgaaac accttggctg aacgatgctc aaaacgaaga cgagaaaaag aaaaatctag
     9601 ccttgctttt cgatttagaa aaaatgaaaa gttcacagga agccactttc gacaaactga
     9661 aacaaaaaca aaaaccatca ggaggttttg cttggtttga cggaagcgag gaaagtgaat
     9721 acattacaag acatattttg gcaggcttgg ggcatctttc caaactcact aaagacgaaa
     9781 acaatgctac caaaatcaag gaaattgcca caactggagt tccgtttatg gatgcaaaat
     9841 ttttagaaaa ttataaaaac acgaccaaaa actggaagaa aaccgataag ttgatttggt
     9901 tcaatccgta ttccgatttg cattatttat acacccgagg tttttatttg gaaaattatc
     9961 cactttcgga tactttgaaa aaagcaacta aattgtatct tgaagctgcc aaaaaagatt
    10021 ggctgaccta ttctttatac gaaaaaggat tggctgcttt gactttacat cgctttggtg
    10081 aaaaagacgc agccaagaaa atcatcgaaa gcctgaaaga aacggcttcc aacaatgaag
    10141 attggggaat gtattggatt gccaacaaag caggctggta ttggtatcaa gctccgatag
    10201 aaacccaagc tttattaatt gaggctttcg ccgaagtttc gaacgacacc aaatcggtcg
    10261 atgcaatgaa agtttggttg ctgaaaaaca aacaaaccaa aaactggccc accacaaaat
    10321 cgaccaccga agctatttat gctttattgt tgcaaggaac gaattggttg agcgtaaaag
    10381 acaacaccat tatcaaaatt ggcgaccaaa aaatcctgac gaaaaaacta tccgaaaacg
    10441 aaaaagaagc cgaaacaggt tatgtaaaac taaactggaa agccgacgaa atcaagaaag
    10501 aaatggcaac tattgttgtt gaaaacaaat cgaaagttcc cggttttgga ggagtttatt
    10561 ggcaatattt tgaagatttg gataaaatta aaaccaattc aggagccgtt ttatcggttt
    10621 ccaaagaatt gtatctcaag aaaagcacac taaaaggcga agaattgcaa aaaataacta
    10681 ccaaaaacag tttgaaaatt ggcgatttag ttaccgttcg attagttatc acttccaaag
    10741 aagatatgga atttgtacat ctcaaagata tgcgtgcttc ctgttttgaa cctgtagatg
    10801 tattgtcgag tttcgaatac aaagcgggat tgggttttta taaaagcaca aaagacgccg
    10861 caacacattt cttttttgac accatcaaca aaggaactta tgtcttagaa tacgacattc
    10921 gagtaaacaa tttgggcgat ttctctaacg gaattacgac tatcgaaagt atgtatgcac
    10981 cagaattcag cagtcatact aaaggcattc gagtgaatgt gaaggaataa aaccttcatc
    11041 caataaacaa atccatctcg ttagatttta acgagatgga ttttttattt gcaataatca
    11101 taacactaaa tcaacattca cataacactt gcctcctact ttcgtaaaaa ctttttatga
    11161 aaagaaagag aaaattacct ttagtagtca taagcgatat tcaccttggc acttacggct
    11221 gtcacgccaa agaattactg caatatttaa aatcaatcca tccacagacc ctggttttga
    11281 acggcgatat tatcgatatg tggaatttca gcaaaagcta ttttcctgct gcacatatgg
    11341 aagttttgcg atacatcatc aagatgtcca atttaggaac gcgagtaatt tacattaccg
    11401 gaaaccacga cgaagcctta cgaaaatact ccgattttat tcttggaaat ctggaattgg
    11461 ttgacaaatt aattttggac ttagacggaa aaaaaacttg gatattccac ggtgatgttt
    11521 tcgattcttc cacaaaaggc tatgccaaaa tattagccaa attaggcgga aaagggtatg
    11581 atttattaat tttaatcaac agttttatca attgggtttt ggttgcttgt ggaagagaaa
    11641 agagaagttt ttccaaaacc atcaaaaata gcgtaaaaaa agcagtttct tttgt
//
LOCUS       DAOSNN010000052        11662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5490_length_11662_cov_5.284828, whole genome shotgun sequence.
ACCESSION   DAOSNN010000052 DAOSNN010000000
VERSION     DAOSNN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11662
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5490_length_11662_cov_5.284828"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLQ70_04195"
     CDS             <1..411
                     /locus_tag="PLQ70_04195"
                     /inference="COORDINATES: protein motif:HMM:NF026975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33849.1"
                     /translation="FTNTVKNIADYVLSNPKILATLKHYTGLTGIEIYEKLQYGQGPT
                     VMITQLNNSYGYHNPFTNTVEIDIDYVQNLENSTGNSAELLNLMLSITLLHEFVHWTD
                     GLFFDYSQENGNNWEIGTYGFVVNMGNVSIIREP"
     gene            430..945
                     /locus_tag="PLQ70_04200"
     CDS             430..945
                     /locus_tag="PLQ70_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33850.1"
                     /translation="MIKKIIIIVGIICLGIYLYPSALNLDNNKPSDVEILLSKSLSSK
                     KISLNTLNLHRVIFNEINGVKVNYNLLGVKFLSTKELLKNKNDKFLSVNLKIQKIRAT
                     VQISYFKSGISYSFHYKKKGETWIETNSEWGFVKFEPSKPYYIYWEIIRLKNPDYMDF
                     NAFGIPKDSLK"
     gene            1109..3481
                     /locus_tag="PLQ70_04205"
     CDS             1109..3481
                     /locus_tag="PLQ70_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33851.1"
                     /translation="MKHSIYIFAFLLTTTISFSQNTDNQQKIKINLEDYFHFERENIH
                     VQFNKTIYISNENIAFKGYVFNKQSSFPNLNTTNVQLAIYDAQGQVIQKQLLHTSLGT
                     FDGIVTLDEKIASGKYRFHFYTNWMNNFMEDESFAQNIEIINLKESYNLKTNQPNYNA
                     VSIFFNPESGSFIKGISNTMGIQIKDCNDLGIQIIEGLVVDSKGKTITTFFTNKMGYG
                     KFELTPDSNETYSVKIATDKLKMEKQLPKATNSGLAITYNNNLPNNMLEVSVKTNSDG
                     VNLYQNKNYTLLIHQNGKYIQKEFNFNDKNLIQILIFDKNELSNGVNSIRIIDENLNE
                     IAERLLYLNKQPKEIPALETQFTANDSIHLFGKINSKVANLSISILPTETACLDQKNS
                     ILGTLNLNAYLEKPVLNNYIYFDRQNVTRAFDMELLMLNQTKSKYFWNNIMSHKPMES
                     YTFTKGITINGTIVKPLNPKTKYKVALISLKNKIFEEAILDKNNKFSFDNFFARDSTA
                     YQLELMDEKNLAIETKMIVNILNNDQPFLKKNFFDGANCQPIKDLNRTFAFSNADLEK
                     KRIALNEIVVQNNYKKPIYIHQTEMNNQAAKAYKMGETDFGSVLDFIGRNGYVTGVDQ
                     ENSTYVRSSRHEYSGNESVATPAIFVDNFQILDLNVLYDLDLMNVDEIYIDKIGTSTT
                     ATGGYGTIRIYLKKGVNNSYLKKKHTTLIVKSGFAKSKTFKNIEFPFSREFYLFGTLQ
                     WSPSVFLDANQFFEVSFPKINQKEVLVVIEGFTIEGQLISEIKKLSTIAK"
     gene            complement(3538..4215)
                     /locus_tag="PLQ70_04210"
     CDS             complement(3538..4215)
                     /locus_tag="PLQ70_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQE33852.1"
                     /translation="MNNFDWTQLINPEFYILMEFGGVKMGLLVILFIIFAETGLFAGF
                     FLPGDSLLFLAGIYSELLTRQIAVGNEFANVVLLSALVSAAGVFGNMVGYWFGVKGGT
                     YLYNKEDTFWFKEKYLLQSRDFFDKYGGKAIIFARFLPIFRTFAPIVAGIAAMDKKKF
                     MFNNILGSVLWSFSMIFAGHYLSAMFLDKFGIDLKKHIEYIVIGIVLVTTLPVLWKLF
                     KKRVHIE"
     gene            complement(4397..4738)
                     /locus_tag="PLQ70_04215"
     CDS             complement(4397..4738)
                     /locus_tag="PLQ70_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HQE33853.1"
                     /translation="MKNLFLGMMLLVVTLSVQAQTKKNKNAKYTIEVNGNCEQCQNRI
                     QKAAFSVSGVKMASWDIDSHKLNVTINEEKTSIKAVEVAIAKVGHDTKEFKSTKESYD
                     KLPGCCQYDRW"
     gene            complement(4798..6825)
                     /locus_tag="PLQ70_04220"
     CDS             complement(4798..6825)
                     /locus_tag="PLQ70_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE33854.1"
                     /translation="MKKQISILLLSLLCAFTSYSQDTLKVVKVVSKSKGLQKSSKITA
                     NTTLVTSKELLKAACCNLAESFETNPSIDVNFSDALTGTKQIKMLGLTSPYLMIDEEN
                     IPSVRGASQAYGLSFTPGTWIESIQITKGAGSVVNGYESISGQINTELIKPINNIPFF
                     LNAYGSSDSRFELNTHFNAKLSDKWSSALFVHGNARTAKKDMNDDGFLDNPLAKQVNI
                     LNRYQYYNAEKGWVSFINFRYMNDKKQAGETDFDPNKDKGTTNFWGSEINTERFDVSS
                     KVGYVFKDMPYQSFGFQNAFNSHNQDSYYGLNQYNIKQSSFYSNLIFNSIINNTMHKF
                     ATGLNFTYDKYQEFVNVNDYSRIDNSIGAFFEYTYDNASNFSLIAGGRVDNHNRLGTF
                     FTPRLHIRYNPWEKATLRVSAGRGKRSANIFAENQSLFASSRTFDVLNTSGKIYGLDP
                     EIAWNYGLSFNQKFKLFGRNSDIGFDFYKTDFTKQVVVDLYTAPAISGTHQVLFYNLN
                     GKSFANSLQIDFNVELVKHLDIRTAYKYYDIQTDYLSGSSERPLQAKHRFFANLGYET
                     HITDKGKQWKFDFTYNWMGQQKLPETASNPIEDRLPVYSPSYSLMNFQITRTFSSTFE
                     MYFGGENVGNYKQEKAILGSENPFGSTFDASIVYAPVFGQMYYAGLRFKIK"
     gene            complement(6901..7326)
                     /locus_tag="PLQ70_04225"
     CDS             complement(6901..7326)
                     /locus_tag="PLQ70_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33855.1"
                     /translation="MKFKKHISFLLALFLLVSNTGFAIDVHYCGGKIASIKPAYLESK
                     EVQSLKKESCCPPKSSNIVNKKESCCKDKVVHFQKKSGKVTLSSISFQPDFTFIFEEW
                     NPIVFSEYSNFESNCILSYYCDANAPPLFKLYHQYVFYA"
     gene            complement(7372..8727)
                     /locus_tag="PLQ70_04230"
     CDS             complement(7372..8727)
                     /locus_tag="PLQ70_04230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQE33856.1"
                     /translation="MKIKFIGGAGTVTGSKTVVESNGVRILIDCGQFQGIKSLRELNW
                     LPLPVQPSTIDFVLLTHGHLDHCGWLPRLVDQGFKGKIYCTSPTKDITKLILLDSAKI
                     QEEEAYKANKEHYSKHEIAEPLYDLKKAEAVFSLFRVIKPNEKIDLDAEISAVFTNAG
                     HIVGACSIELNLENKTVVFSGDIGRDNDILMYPPTKPQKADYVFLESTYGNRLHPETD
                     AKAELEIYINNTIQKGGTVIIPSFAVERAQTIMYLLWQLKTEDRIPNIPYIIDTPMGI
                     KVLDIFENNRRWHKLPEHEYVAMCQMFTMNTDYQETIDTIYNPQPKVIIAASGMITGG
                     RVLSYLERYIGLPETTVIIIGYQAEGTRGRKLLEGDKEIKIYGKQYEVKANILEIEGL
                     SAHGDQKDLVNWLSELQNKPQKVFLVHGEKEAFEELSTKITEKYGFECVIPQMNQEFD
                     I"
     gene            complement(8785..9615)
                     /locus_tag="PLQ70_04235"
     CDS             complement(8785..9615)
                     /locus_tag="PLQ70_04235"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HQE33857.1"
                     /translation="MKVVKHCGDIVDFDPIKLKASLLKSGANKLVVETILETIKKELY
                     EGISTKQIYKRAFGLLKKAANSHAARYNIKEAIRLLGPDGFFFEKYIARLFASEHYQT
                     KTNLILQGKCVTHEIDVLAKKDQIISMIECKFHMGKDTNSDVKVPMYILSRFNDLKEK
                     RHTIFSVNEIISKCWIVTNNRFTSDAITFAKCSGLNLLSWNYPENNNLSTKNAENFLY
                     PVTCLTTLTLVEKDRLLILDVILVKELINNSDCLEKLGLSSNRIKNVLKEASELCEYI
                     "
     gene            complement(9733..10497)
                     /locus_tag="PLQ70_04240"
     CDS             complement(9733..10497)
                     /locus_tag="PLQ70_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQE33858.1"
                     /translation="MKKAFKKILNIVPRPLLIRLSYVVRPFIAFSLKGDKYTDPIDGK
                     SFKSMLPYGYETQRNNVLSPSTLSLERHRLLWLYLNEQTDFFTSKKKVLHFAPEQAFY
                     KLFRNQQNLDYTTTDLFSPLADVKADICNLPFEDNQYDVILCNHVLEHIPDDTKAMQE
                     LYRVLKPGGMAILQIPQDLKREVTFADDSITDQKERAKIFGQYDHVRIYGRDYFDKLR
                     SIGFTVVEEDYTNKIAPELVEKYCLAKGEIIPVCFK"
     gene            complement(10621..11439)
                     /gene="map"
                     /locus_tag="PLQ70_04245"
     CDS             complement(10621..11439)
                     /gene="map"
                     /locus_tag="PLQ70_04245"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQE33859.1"
                     /translation="MIIQKTREEIELMRESALIVSKTLGMIASEIKEGVTTLYLDKRA
                     EEFIRDHGAVPSFLGLYGFPNSLCMSPNAQVVHGIPNNTPLQNGDVISVDCGAFKNGF
                     HGDHAYSFEIGEVAPETKKLLQVTKESLYVGIRELRIGNRVEDVGNAIQKYTEAHGYG
                     VVRELVGHGVGQKMHEDPEMPNYGKRGRGKLLIEGMVVAIEPMINMGTKNIKQHKDGW
                     TITTADGKPSAHFEHDVAIIDGKPEILSTFAYIYKELGIVSNEEDEFRKVPLVV"
     gene            complement(11482..>11662)
                     /locus_tag="PLQ70_04250"
     CDS             complement(11482..>11662)
                     /locus_tag="PLQ70_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQE33860.1"
                     /translation="DNQTMLQSEERLFSFGESSLFLINTRENNLVSAQLAQINLANRF
                     YISNSELFKIMANPN"
BASE COUNT         3938 a         2050 c         1786 g         3888 t
ORIGIN      
        1 tttactaata ctgtgaaaaa cattgcagat tatgttcttt caaatccaaa gattttggca
       61 acattaaagc attacacagg gttgactggt atagaaatct atgaaaaact tcaatatgga
      121 caaggtccga cggtaatgat aacccaatta aataattcat atggatatca caacccattt
      181 acaaatacag ttgagataga tattgactac gtacaaaatt tagagaattc aacaggtaat
      241 tcagccgaac ttttaaattt aatgctttct ataacattgc ttcatgaatt tgttcattgg
      301 acagatggat tgttttttga ttactcacaa gaaaatggaa ataattggga aattggaaca
      361 tatggatttg tagttaatat gggcaatgta tcaatcatac gtgaacctta aaaaaatatc
      421 aaatatttta tgataaagaa aataattatt atagttggga taatatgttt aggtatatat
      481 ttatatccga gtgctctaaa tttagacaac aacaaaccta gcgatgtaga aatattactt
      541 tctaaatcac tgtcttctaa aaaaattagt ttaaatacct taaatctcca tcgagtaatt
      601 tttaacgaaa taaatggggt aaaagttaat tacaatttgt taggtgttaa atttctatct
      661 acgaaagaat tattaaaaaa taaaaatgat aaatttcttt cagttaactt aaaaattcaa
      721 aaaattagag ccactgtaca aattagctat tttaagtctg gaatatctta tagttttcat
      781 tataaaaaaa agggagaaac ttggatagaa acaaatagtg aatggggttt tgttaaattc
      841 gaaccatcaa aaccttatta catttattgg gaaattataa ggcttaaaaa ccctgattat
      901 atggatttca atgcttttgg cattccaaaa gacagtttaa aatagtttac tattaataga
      961 cgattttgca aaaatatttt caatttttta tctaaaaaaa tcaataacca aaaaatcctg
     1021 ctcaaatcat aagcaggatt ttacttataa atatatttta cattcaagaa atctttttgt
     1081 acttttcaac ttcattaaac acctaaaaat gaaacactca atttacattt tcgcattcct
     1141 actgacaaca acaattagtt tttctcaaaa tacagataac cagcaaaaaa taaaaatcaa
     1201 tttagaagat tattttcatt tcgagagaga gaatattcac gtacaattca acaaaacaat
     1261 ttatatctct aacgaaaaca tcgcttttaa aggctatgtt tttaacaaac agagcagttt
     1321 tcccaacttg aataccacca atgtacagtt agcgatttat gatgcacaag ggcaagtaat
     1381 tcaaaagcaa ttgcttcaca cttcgctagg aacttttgac ggaattgtaa cattagacga
     1441 aaaaatagct tctggaaaat accgctttca tttttacacc aattggatga ataattttat
     1501 ggaagacgaa tcctttgctc aaaatattga gataataaac ctaaaggaat cttataattt
     1561 aaaaactaat cagcccaatt ataacgctgt ttctatattt tttaatccag agagtggctc
     1621 atttattaaa ggaatcagca acacaatggg tattcaaata aaggattgca acgatttagg
     1681 aattcaaatc atagaaggat tagtggtaga ttcgaaagga aaaaccatca cgactttctt
     1741 taccaacaaa atgggttacg gaaaatttga attgactcca gattcaaacg aaacctactc
     1801 ggtaaaaatc gcaacagaca aactgaaaat ggaaaaacaa ttgcccaaag caaccaattc
     1861 gggccttgcc ataacctaca ataacaattt acccaacaat atgttggagg tttctgtaaa
     1921 aaccaattcg gatggcgtga atttatacca aaataaaaat tacactttgc tcattcatca
     1981 aaacgggaaa tacattcaaa aagaatttaa ttttaatgac aagaacctaa tccaaatcct
     2041 aattttcgat aaaaatgagt tgtcaaatgg ggtgaatagc attcgaatta ttgatgaaaa
     2101 tctaaacgaa attgccgaaa gattgctcta tttgaacaag caacctaaag aaattccagc
     2161 attggaaacg caatttactg ccaacgacag tatccactta tttggaaaaa taaattcaaa
     2221 agtggccaat ttaagtatca gtattttgcc aacagaaacg gcttgtctcg accagaaaaa
     2281 ttcaatttta ggaacgttaa atctcaacgc ttatttagaa aaaccagtac ttaacaacta
     2341 catttatttt gaccgccaaa acgtaactag ggcttttgac atggaattat tgatgctcaa
     2401 tcagactaaa agcaaatact tttggaacaa tattatgagc cataaaccaa tggagtccta
     2461 tacatttacc aaaggaatta ccatcaatgg aaccatcgta aaaccattga acccaaaaac
     2521 aaaatataaa gttgcactaa tctctttgaa aaataaaatt tttgaagaag caatcctgga
     2581 caaaaacaac aagtttagtt ttgataattt ttttgcaaga gattctacag cctatcagct
     2641 agaattaatg gacgaaaaga acctagccat tgaaacaaag atgattgtca acattttgaa
     2701 caatgaccaa ccttttttaa aaaaaaattt ttttgatggt gccaattgtc aaccgataaa
     2761 agatttgaat agaactttcg ccttttcgaa tgccgattta gaaaaaaaga gaattgcatt
     2821 aaacgaaatt gtcgttcaaa ataattataa aaaaccaatc tacattcatc aaaccgaaat
     2881 gaataatcag gctgcaaaag cctataaaat gggagagaca gattttggtt cggtcttgga
     2941 cttcattggc agaaacggtt atgtcactgg tgtcgaccaa gaaaattcga cctacgttcg
     3001 cagttctaga cacgaatatt ctggaaacga aagtgtagct actccagcta tttttgtaga
     3061 taattttcaa attttagatt taaatgtttt gtatgacctt gatttaatga atgtcgatga
     3121 aatttatatt gacaaaatag gaacttccac tactgctact ggaggttacg gaacaattag
     3181 aatttaccta aaaaaaggtg tcaataatag ttatttgaaa aagaaacata caactctaat
     3241 cgtgaaatct ggttttgcaa aatctaaaac attcaaaaac atagaatttc cattttcgag
     3301 agaattttat ctttttggaa ctttgcagtg gtccccaagt gtgtttctag atgccaacca
     3361 atttttcgaa gtttcctttc ctaaaataaa tcaaaaagag gttttagttg tgattgaagg
     3421 gtttacaatc gaaggtcaat taatctctga aataaagaaa ttaagcacaa ttgcaaaata
     3481 ataaataaca aaaaaaactc cgaagtttaa aagcttcgga gttttttcat aaatctttta
     3541 ttcaatatga actctttttt tgaataattt ccaaagcact ggaagcgttg ttacaagcac
     3601 aattcctata acaatatatt cgatatgttt tttcaaatca ataccgaatt tgtctaaaaa
     3661 cattgctgac aaataatgcc ctgcaaaaat catactgaaa gaccatagga cagaacccaa
     3721 aatattatta aacataaatt tctttttatc catcgcagca attccagcta caatgggtgc
     3781 aaaggttctg aaaataggta aaaaacgagc aaaaataatc gctttccctc catatttatc
     3841 gaaaaaatct ctcgattgta aaaggtattt ttccttaaac caaaacgtat cttctttatt
     3901 ataaagataa gttccgcctt tcactccaaa ccaatatcct accatattac caaaaactcc
     3961 tgctgctgag actaaagctg atagcaatac aacattcgca aattcattgc ccaccgcaat
     4021 ctggcgagtt aataattcgc tgtaaatccc tgctaaaaac aacaaactat ctcctggaag
     4081 aaaaaaccct gcaaacaatc cagtttcggc aaaaattatg aataggatta ctaacaatcc
     4141 catttttact ccgccaaatt ccatcaaaat ataaaattct ggatttatta gctgcgtcca
     4201 atcaaaatta ttcatataaa tattttaaaa attctaggtt gtgaaagtaa ttattattta
     4261 gcaaaacagc actatctagt caatggattt gcagtttttt aataaatatt taataaaaac
     4321 accattcaat tgaaaactta aactaacctc ataaaaagga attccaaaaa tgttggaatt
     4381 ccctttctta ccttgactac catctatcat attgacaaca acctggtagt ttgtcataac
     4441 tttctttggt ggatttaaac tctttagtat cgtgtcccac ttttgcaatt gcaacttcta
     4501 cagctttaat cgaggttttc tcttcattaa ttgtcacatt tagtttatga gaatcaatgt
     4561 cccaactcgc catttttaca ccactgaccg aaaaagccgc tttttgaatt cggttttgac
     4621 attgttcgca attgccattc acttctatgg tgtattttgc atttttattc tttttagttt
     4681 gtgcttgtac agaaagtgtt actactaata acatcattcc taaaaataga tttttcatat
     4741 tttacttttt taattgttat tgaaattgat tttttgtcat tgtcattgac attgttgtta
     4801 ttttattttg aatcgaagtc ccgcataata catttgccca aaaacgggcg catatacaat
     4861 agatgcatcg aatgttgagc caaaaggatt ttcagaacct aaaattgctt tttcttgttt
     4921 gtaatttcca acgttttctc cgccaaaata catttcgaaa gttgaagaaa aagttcgagt
     4981 aatttgaaaa ttcatcaaag aataggaagg cgaataaacc ggaagtctat cttctattgg
     5041 gtttgatgcc gtttctggca gtttttgttg tcccatccag ttgtaagtga aatcaaattt
     5101 ccattgtttt cctttatcag taatatgagt ttcatatcct aaattagcaa aaaatcgatg
     5161 tttggcttgc aaaggtcttt cgctacttcc gctcaaataa tcggtttgaa tgtcataata
     5221 tttataagca gttcggatat ccaaatgttt cactaattct acattaaaat caatttgcaa
     5281 actattggca aacgattttc cgttcaaatt gtaaaacaaa acttgatgcg ttcccgaaat
     5341 tgctggagcg gtatataaat cgacaactac ttgttttgta aaatcggttt tgtaaaaatc
     5401 aaaaccaatg tcggaatttc gtccaaaaag cttaaacttt tgattaaaac tcaatccgta
     5461 attccaagcg atttcaggat ctaaaccata aatttttccg ctagtattca agacatcaaa
     5521 ggttcttgaa ctcgcaaaaa gcgattgatt ttcagcaaaa atattggcac tacgtttccc
     5581 tctaccagcc gaaacacgca aagtagcttt ctcccaagga ttgtaacgaa tgtgcaaacg
     5641 tggcgtaaaa aaagtcccca atcgattgtg attatccact cttccacccg caattaaact
     5701 gaaattcgaa gcattatcat acgtatattc gaagaaagct ccaattgaat tatcaattct
     5761 actgtaatca ttcacattga caaattcttg gtatttatca taagtaaaat ttaaacctgt
     5821 ggcgaatttg tgcatcgtat tattaataat cgagttgaaa attaaattcg aataaaaact
     5881 actttgcttg atattgtatt gatttagtcc ataataagaa tcttgattat gactgttaaa
     5941 agcattttga aaaccaaaac tttgatacgg catgtcttta aacacataac ctactttcga
     6001 cgaaacatca aagcgttctg tattgatttc agaaccccaa aaattagttg ttcccttgtc
     6061 tttatttggg tcaaaatcgg tttcacctgc ttgtttctta tcattcatat atctgaaatt
     6121 aatgaaactc acccaacctt tctcggcatt ataatattga taacggttta aaatattgac
     6181 ttgttttgcc aacggattat ccagaaaacc gtcatcattc atatcctttt tggccgttct
     6241 tgcatttccg tgaacaaaca acgcacttga ccatttatcc gaaagtttgg cattgaaatg
     6301 cgtatttaat tcaaaccgag aatcggaaga gccgtaagca ttcaagaaaa aaggaatatt
     6361 atttatgggt ttaattaatt cggtattgat ttgtcctgaa atactttcgt atccattgac
     6421 aacactgccc gcaccttttg tgatttggat actttcaatc caagttcctg gtgtaaatga
     6481 cagcccataa gcttgtgaag caccacgaac agaaggaata ttttcctcgt caatcatcaa
     6541 atatgggctc gttaaaccca gcattttgat ttgttttgtc ccagtcaatg cgtccgaaaa
     6601 attgacatct attgatggat tggtctcgaa actttcggcg agattgcaac aagcggcttt
     6661 gagtaattcc ttgctagtta ccaatgttgt atttgcggta atcttagagg atttttgtaa
     6721 gcctttgctt ttagaaacta cttttaccac ctttaatgtg tcctgagaat aggatgtaaa
     6781 cgcacacaac aagctcaata aaagtattga aatttgtttt ttcataaaaa ttgattttaa
     6841 tgttttgtaa tcagaaatat caaaccaaaa gaaggtttga tttcaaaaaa aacattaaaa
     6901 tcaggcgtag aaaacgtatt gatggtataa tttgaaaagt ggtggcgcat tggcatcaca
     6961 ataataagag aggatgcaat tgctttcaaa atttgaatat tcagagaaaa cgataggatt
     7021 ccattcttcg aatataaaag taaaatcggg ctgaaaagag attgaactta aagttacttt
     7081 tccagatttt ttctgaaaat gaactacttt atccttgcaa caactttctt ttttgttgac
     7141 aatgtttgaa gattttggag gacaacaact ttcctttttt agactttgta cttctttaga
     7201 ttctaaatat gcgggcttga tagaagctat tttccctcca caataatgca catcaatagc
     7261 aaatcccgta ttggaaacca acaggaataa agccaataaa aaactgatgt gctttttgaa
     7321 tttcatagta caaatttaag tatttaaatc cttcctaaaa taaaaattat gttaaatatc
     7381 aaactcttga ttcatctgtg gaataacaca ttcaaaaccg tatttttcag tgatttttgt
     7441 gctaagttcc tcaaaagctt ccttttcgcc gtgaaccaaa aatacttttt ggggcttgtt
     7501 ttgcaattcc gaaagccaat taactaaatc cttttgatcg ccgtgagccg acaaaccttc
     7561 tatttcaaga atgttagctt ttacttcgta ttgtttgccg taaattttaa tttctttatc
     7621 tccttctaac aattttcttc cacgagttcc ttcggcttga tagccaatga tgattacagt
     7681 agtttcaggc aatccaatat aacgttctaa ataactcaaa actctacctc cagtaatcat
     7741 tccgctggct gcaatgatta ctttgggttg aggattgtaa atagtatcaa ttgtttcttg
     7801 ataatctgta ttcattgtaa acatctgaca cattgcaaca tactcgtgtt cgggcaattt
     7861 gtgccatctt ctattatttt cgaaaatatc cagcacttta attcccattg gagtatcaat
     7921 tatataggga atatttggaa tcctatcttc ggttttgagt tgccacaaca aatacattat
     7981 cgtttgtgct cgctctacag caaaactcgg aatgataacc gttccgcctt tttgaattgt
     8041 attgttgatg taaatttcca attcggcttt ggcatctgtc tcaggatgga gtctatttcc
     8101 gtatgtgctt tcaaggaaaa cataatcggc tttctggggt tttgttggcg gatacatcaa
     8161 aatatcatta tctcggccaa tatctcccga aaaaactacc gttttatttt ctaaattcaa
     8221 ttcaatacta caagctccaa caatgtgtcc cgcattggtg aaaaccgctg aaatttcggc
     8281 atccaaatca attttttcat tgggtttaat cactctaaac agcgaaaaaa cggcttctgc
     8341 tttttttaaa tcataaagcg gctcggcaat ttcgtgttta gaataatgtt ctttattagc
     8401 tttataagct tcttcttcct gaattttagc actatccaaa agaatcagtt tagtaatgtc
     8461 ttttgttgga ctagtacaat aaattttgcc tttaaagcct tgatcgacca atcttggcaa
     8521 ccaaccgcaa tgatctaaat gaccgtgagt caacaacaca aaatctatgg ttgaaggttg
     8581 aactggcaag ggcagccaat tgagttctct taaagattta ataccttgaa attgtccgca
     8641 atcgatcaaa attcgaaccc cattactttc tactactgtt tttgaacccg ttaccgttcc
     8701 tgcaccgcca ataaatttaa ttttcatgat tcctatattt tatattcaag aaattataat
     8761 tgctaactat attttgccga agttctatat atattcacat agttctgaag cttcttttag
     8821 gacgttttta atacgatttg aactcaatcc taatttttcc aaacagtctg aattgttaat
     8881 taattcttta actaaaatta catctaaaat caataatctg tctttttcaa ccaaggtcaa
     8941 agtggtcaaa caagttacag gataaagaaa attttctgca ttttttgtgc ttaaattatt
     9001 attttcggga taattccagc tcaacaaatt caaccccgag cattttgcaa aagtaattgc
     9061 gtccgaggta aaccgattat tggtaacaat ccaacatttt gaaattattt cgtttaccga
     9121 aaaaatagta tgtcgttttt ctttcaaatc attaaatcga gacaaaatat acattgggac
     9181 ttttacatct gaattagtat cttttcccat atgaaattta cattctatca tcgaaataat
     9241 ttggtctttt ttagccaata catctatttc gtgagtaaca catttgcctt gtaaaatcaa
     9301 atttgtttta gtttgataat gctctgaggc aaaaagtcga gcgatgtatt tttcgaagaa
     9361 aaacccatct ggtcctaata atcgaatggc ttctttaatg ttataccgtg cagcgtgaga
     9421 gtttgctgct ttttttaata gcccaaaggc tcttttgtag atttgcttgg tcgaaattcc
     9481 ttcgtaaagt tcttttttta tggtttccaa aatagtttcg accaccaatt tgttggctcc
     9541 tgattttaag agcgacgctt taagtttaat gggatcaaaa tcgacaatat ctccacaatg
     9601 tttaactact ttcatctgtt agtaatttaa caatttacaa aattatttcc ttactaaagg
     9661 tacgaaaaat aaagagacca ctttgtattt aaaacaaaat tacaaaatga aataaaaatg
     9721 gagattatcg agttatttaa aacaaaccgg aatgatttct cctttggcta aacaatattt
     9781 ttccaccaat tcaggagcga ttttattggt gtaatcttct tcgacaacgg taaaaccaat
     9841 gcttcttagt ttatcaaaat aatcgcgacc ataaatgcga acgtgatcgt attgaccaaa
     9901 aattttggct cgttcttttt gatctgtaat cgaatcatcg gcaaaggtta cttcgcgttt
     9961 taagtcttga ggaatttgta gaattgccat tccgcctggt ttcaaaactc tatataattc
    10021 ctgcattgct ttggtgtcat ccggaatgtg ctccaaaacg tggttgcaca aaatcacatc
    10081 gtattgattg tcttcgaaag gcaaattgca aatatctgct tttacatcgg ccaagggtga
    10141 aaacaaatca gtggtggtat aatctaagtt ttgttgattg cggaacagtt tgtaaaatgc
    10201 ttgctctggc gcaaaatgta gtactttctt tttggatgta aaaaaatcgg tttgctcgtt
    10261 gagatataac cacaacaaac ggtgtctttc taaagaaagt gtacttggtg aaagcacatt
    10321 attgcgctgt gtttcgtatc cataaggtaa catcgatttg aaactttttc catctattgg
    10381 atcagtgtac ttgtctcctt ttaatgaaaa agcaataaat ggacgaacta cataactcag
    10441 tcttattaat aatggacgag gaacgatatt aagaattttt ttaaaagctt ttttcatagt
    10501 tgttaagcgg agattcctaa aagtttttta tgtgttttct taaatgacct taatatctta
    10561 tatggttaaa taattcgtca actatataag gcatttaagg ttatttaagt tttctatttg
    10621 ttagacaacc aaaggcactt ttctaaattc atcctcttca ttactcacaa tgccaagttc
    10681 tttgtagata taagcaaaag tcgataaaat ctctggttta ccatcaataa tggctacatc
    10741 gtgttcgaaa tgggccgatg gttttccgtc ggcagtggtg atggtccaac cgtctttgtg
    10801 ctgtttgatg tttttggttc ccatattaat cattggttcg atggcaacca ccattccttc
    10861 gattaaaagt ttgcctcgac ctcgttttcc gtagttaggc atttctggat cttcgtgcat
    10921 tttttgtcca acaccgtgac ctactaattc acgaaccacg ccatatccgt gggcttcggt
    10981 gtatttttgg atcgcattac ctacatcttc tacacggttt ccgattctta attctcggat
    11041 tccaacgtat agtgattctt tggtaacttg caataatttt ttggtttctg gagctacttc
    11101 tccaatttca aacgaataag cgtgatcacc gtgaaatccg tttttgaaag caccgcaatc
    11161 taccgaaatg acatcgccat tttgcaaagg cgtgttgttc gggattccgt gtaccacttg
    11221 agcatttgga ctcatacaaa gcgaatttgg aaagccatac agaccaagaa aacttggaac
    11281 agctccgtga tctcgaatga attcttcggc tcttttgtct aaatataaag tggtaactcc
    11341 ttctttaatt tcagaagcta tcattcctaa tgttttggat acgattaagg cactttcgcg
    11401 cattaattct atttcttctc gtgttttttg gataatcatt tttcgtaaaa taaggcgcaa
    11461 aaatacaact tttaatagta attaatttgg attcgccata attttaaaaa gttcagaatt
    11521 ggagatgtaa aaacgattgg ccaaattgat ttgagccaac tgtgcgctga ccaaattatt
    11581 ctcacgggtg ttgattaaaa acaacgaact ttcgccaaat gaaaataaac gttcctcaga
    11641 ttgcaacatc gtttgattgt ct
//
LOCUS       DAOSNN010000053        11451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5635_length_11451_cov_5.644349, whole genome shotgun sequence.
ACCESSION   DAOSNN010000053 DAOSNN010000000
VERSION     DAOSNN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5635_length_11451_cov_5.644349"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLQ70_04255"
     CDS             complement(<1..581)
                     /locus_tag="PLQ70_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQE33861.1"
                     /translation="MTEITNYITNDYKAIDSQETIATVQDFFGELTFSHFPVVNEGIY
                     IGSIASEDIETFDSHKKVYDYRYALEGFFAREKMIWLDVLEVFAKNNTDLVPVLNDNN
                     NYLGYYEIRDIIKFFHETPFLQETGGIIVVQKKTLGYSVGEITQIIESNNGKILGLFV
                     SQSDFENTQVTIKISLGIMNEIIQSFRRYNYEI"
     gene            681..1394
                     /locus_tag="PLQ70_04260"
     CDS             681..1394
                     /locus_tag="PLQ70_04260"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQE33862.1"
                     /translation="MTKLSVNINKIATLRNARGGNVPDLLKVATDIQKFGGQGITIHP
                     RPDERHIRYQDARDLKPIVYTEYNIEGNPQHNFIDLVLECKPEQVTLVPDAIGAITSS
                     AGWDTIKNESYLTEVIQEFQRNGIRTSIFVDPIAEMIEGAKKTGTDRIELYTEAFAHQ
                     YGLGNKNGIDPYVKSAVLANELGLGINAGHDLSLDNIQFFHQNIPGLLEVSIGHALIA
                     ESLYLGLENVVNMYLHKLK"
     gene            1466..2227
                     /locus_tag="PLQ70_04265"
     CDS             1466..2227
                     /locus_tag="PLQ70_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQE33863.1"
                     /translation="MIYSKIEGTGKPLLIIHGFLGMSDNWKTIGTQFAADGFQVHLLD
                     LRNHGKSFHSDEFNYEIMAQDVFDYCNANNLEKISILGHSMGGKTAMLFAANYPEMVE
                     KLVVADIGPKFYPQHHQIIFEALNAVDFSLKPSRAEVEEIMSHYITDFGTRQFLLKSL
                     YWKESGQLAFRFNLAVFNLNRDEIGKPLPEDAVFNKPTLFIRGGNSNYILDDDFPKIQ
                     QHFPDSRIETIPNVGHWLHAENPKMFYEMTSLFLK"
     gene            2257..2601
                     /locus_tag="PLQ70_04270"
     CDS             2257..2601
                     /locus_tag="PLQ70_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQE33864.1"
                     /translation="MKLILRIFITAVLVVLIAGAMTSVDVDSFTTAVIVAAVLGLLNI
                     FIKPILVILSLPATILTFGLFLFVINGGIIMLCSRLVDGFRINSFWTAIIFSIILSIC
                     ESIVYKIVGEDK"
     gene            2720..4045
                     /locus_tag="PLQ70_04275"
     CDS             2720..4045
                     /locus_tag="PLQ70_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQE33865.1"
                     /translation="MDIKRVALDAVNETIVMTVVHMDYKGQVAKRINEKMPLAQVKGF
                     RKGAVPKDLVEKQYGKEIKKEEIKKVVDLALERYIQSERLNLLGTPLPKVNENLDWSA
                     EELTFEFEIGLVPSFEIDLDAKNDIIKYVVTADDKLIDGQVARIQKQFGTAIPQDVVT
                     EGADLKGIFTNEAEGIGNTTTIALATFKDKATQDLFIGKKVGDVVTVNTSGLFEDDHQ
                     LMDYLKVGHDNVHGLAIDVDFTIEAITVSEPAELNQELFDKLFGAGNVASLEDLKAKI
                     KEDAESQFAQQADQKLLGDVTEFLIENTKFDLPAEFLKKWLQTVGEKKLSPEEAEVEY
                     ARSEKGLRFQLIEGKALAQSDIKITFEDLKTFTAKNIRQQMAQFGQTNPTDEEVQGIV
                     ARVLSNQEEVKRLSDQVVAEKLLEIFKEKANPTTKEVTYEEFIAASYGE"
     gene            4219..4893
                     /gene="clpP"
                     /locus_tag="PLQ70_04280"
     CDS             4219..4893
                     /gene="clpP"
                     /locus_tag="PLQ70_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HQE33866.1"
                     /translation="MNYGKEFKKFATKHQGVNAMYYDKIVAAMTPTNMTPYIIEERQL
                     NISQLDVFSRLMMDRIIFLGTGIDDQIANIVQAQLLFLESADASKDIQIYLNSPGGSV
                     YAGLGIYDTMQYIKPDVATICTGMAASMGAVLLCAGAAGKRSALPHSRVMIHQPSGGA
                     QGVASDMEINLREMLKLKDELYKIISHHSGKPFDKVYADSERDYWMIADEAKEYGMID
                     EVLRRG"
     gene            4972..6204
                     /gene="clpX"
                     /locus_tag="PLQ70_04285"
     CDS             4972..6204
                     /gene="clpX"
                     /locus_tag="PLQ70_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQE33867.1"
                     /translation="MAKPKLECSFCGRKKPETNLLIAGIDAHICDKCIEQAHGIVVEE
                     LKSSSKNHLPTDLVLRKPKEIRAFLDQYVIGQDQTKKVMAVAVYNHYKRLMQQELHDE
                     VEIEKSNIIMVGQTGTGKTLVAKTIAKMLDVPLAIVDATVLTEAGYVGEDVESILTRL
                     LQAADYDVTKAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQALLKLLEGTVVNVP
                     PKGGRKHPDQKFVEVNTQNILFIAGGAFDGVERIISKRLNRQAVGYSTSKNVDNIDKD
                     NLLQYIIPKDIKDFGLIPEIIGRLPVLTHMDPLDRETLRAILTQPKNALIKQYQKLFL
                     MDEVEFSITDEALDFVVDKALEYKLGARGLRSLCEAILTDAMYELPSSDDKILEIDKD
                     YAEHTLNKNLLKRMEIAS"
     gene            6352..7587
                     /gene="serB"
                     /locus_tag="PLQ70_04290"
     CDS             6352..7587
                     /gene="serB"
                     /locus_tag="PLQ70_04290"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HQE33868.1"
                     /translation="MALVEKEIILLKVSGQDKPGVTAGLTSILANYDAVILDIGQADI
                     HDTLSLGILFEIKAGSSSAAVLKDLLFRAYELGINVKFIPISIPDYEIWVKGQRKQRY
                     IINVLGETLTAAQLAAVTQIMSNQKLNIDSIIRLTGRVSVVEKEEYPRSCVQLSVTGN
                     INDKTLMTASFMEISKELNVDISFQEDNMYRRNRRLVCFDMDSTLIQTEVIDELAELA
                     GVGEQVRAITESAMNGEIDFSESFKKRMALLEGLSEDVLHNVAINLPITKGAHRLMKA
                     LKYYGYKTAILSGGFTYFGDYLQKELGIDYVYANQLEIKDGKLTGKYLGEIVDGQKKA
                     EYLKAIAEKEGININQTIAVGDGANDLPMLNLAGLGIAFHAKPKVKESAATSISSLGL
                     DGVLYLLGYHDRHIDMMQE"
     gene            complement(7675..8583)
                     /locus_tag="PLQ70_04295"
     CDS             complement(7675..8583)
                     /locus_tag="PLQ70_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQE33869.1"
                     /translation="MNNKEGNNKKSGGRANSSRPSSNKPKPAMPKRAQGPKKVKPAVK
                     IAEAEAAKTAKKPNQAPKRQKEKDEIRLNKYIANSGVCSRRDADIYIQSGNVKVNGIP
                     VTEMGYLVKPSDDVNFDGVKLTPEKKVYILLNKPKNFTTALDEGQEYRNVLELVKGST
                     TAKIGAVGRMDKNTTGLLLFTNDTDMIRKFTLPTNKSSKIYQVSLDKNLKFEDLEKIQ
                     KGLVLDGHRVFVEEVSYIEGEAKSEIGLKLRSSNVKVVRSIFEHFSYDVLRIDRVAFA
                     GLTKKNLPRGNWRLLTEQEIINLKNS"
     gene            complement(8739..9599)
                     /locus_tag="PLQ70_04300"
     CDS             complement(8739..9599)
                     /locus_tag="PLQ70_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33870.1"
                     /translation="MNKVLPLLLTLLSFCVFGQKNPTKEAQPIVAEGKLLYKSEMASW
                     YGTDLFLENYKNRENIGGYFSYTDNDVAKCIFFSKSENPKVIGTISFDSTYNTKTANT
                     NLTERNFTTTENDLYEIRKLALVEIQSDTLFKNYNNSSLNLIPIINGKEKKVYVLTGP
                     KNNGVVIFGNDYLLTFNKDNKLMVKKQLHKNIIPIEYGGKSEEGKEIEGTIHSHLPET
                     GDFITATDICTLMLYEKFTKWKTHNVVSKKYLNIWNCKTDELNVISMDVIEKINKDQE
                     TRNKQKDKTE"
     gene            complement(9764..10681)
                     /locus_tag="PLQ70_04305"
     CDS             complement(9764..10681)
                     /locus_tag="PLQ70_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HQE33871.1"
                     /translation="MNRKSKILLMKIASLFSVVRGYNIPIIVLAQYLSAIFILAPEKR
                     ALSILFDFDLFIIVFASSLTIASGYIINNFYDSKKDLINRPTKSMLDRLVSQKTKLQV
                     YFTLNFIVALLAIIVSWRAFLYFSVYIFLIWYYSHRIKKYPIIGNLTAAFLAILPFFA
                     ILLYYKNFYEVIFGHAVFLFLLILIREMIKDLENIKGDLANDYNTIPILYGEETSKKI
                     ITILTISTIIPVYVLIEIFDVGYMDIYFYASFIILIFFLLRLWKSNTKEQYILLHNVL
                     KFLIVAGVFCIILINPSVLWHGEKLLRSI"
     gene            complement(10767..>11451)
                     /locus_tag="PLQ70_04310"
     CDS             complement(10767..>11451)
                     /locus_tag="PLQ70_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HQE33872.1"
                     /translation="MYFDSSIPQGYGVGSSGALVAAIYDKYAQNKITVLENLTREKLL
                     QLKTIFGQMESFFHGKSSGLDPLNSYLSIPILINSKDHIEATGIPTQSLQGNGAVFLI
                     DSGIVGETAPMVNIFMEKLKDQGFRTMLKLQFIKHTDACVENFLGGDMKSLFANTKKL
                     SKVVLNHFKPMIPEQFHGIWQEGIDSNDYYLKLCGSGGGGYILGFTQDLEKAKRALKE
                     YKLEVVYQF"
BASE COUNT         3771 a         1843 c         2189 g         3648 t
ORIGIN      
        1 ataatttcgt agttgtatct tctgaaagat tgaataattt cgttcattat tcctaaactt
       61 attttaatgg ttacttgtgt gttttcaaaa tcagattgtg aaacaaataa ccccaatatt
      121 ttaccattat tgctttcaat gatttgagta atttctccaa ccgagtatcc taaagtcttt
      181 ttttgaacca caattattcc gccagtttct tgtaaaaaag gagtttcatg aaaaaatttt
      241 atgatatctc taatttcata ataccccaaa tagttgttat tgtcgttcaa aacaggaact
      301 aaatcggtgt tatttttagc aaaaacttcc aaaacgtcta gccaaatcat tttttctctg
      361 gcaaaaaaac cttcgagagc atatctgtaa tcgtaaactt ttttgtgact atcaaatgtt
      421 tcaatgtctt cagatgcgat gctaccaata tatattcctt cattaaccac agggaaatgt
      481 gaaaaagtca attctccaaa aaagtcttga acagttgcaa tagtttcttg actgtcaatc
      541 gctttataat cattggtgat atagtttgta atctcggtca taaattaatg ctaaatattt
      601 aatgcaaaat aatcaaaaat atgtaataac agctagtttt actttgtatt tttgcccctc
      661 aatacctgaa atttattcaa atgacaaaat taagcgtaaa tattaacaaa atagcaactt
      721 tacgaaatgc tcgtggcgga aatgttccag atttactaaa agtagcaact gatattcaaa
      781 aatttggagg tcagggaatc accatacatc cacgacctga cgaaaggcac attcgctatc
      841 aagatgctcg agatttaaaa ccgatagttt ataccgaata taatatcgaa gggaatcctc
      901 agcataattt tattgacttg gttttagaat gtaaacctga gcaagtgact ttagttccag
      961 atgctatcgg tgcaattact tcttcggctg gttgggatac aataaaaaac gaatcctatt
     1021 taactgaagt tattcaagaa tttcaacgca atggaattcg aacttcgatt tttgtcgatc
     1081 caattgcgga aatgatcgaa ggtgccaaaa aaacaggaac cgatagaatc gaattgtaca
     1141 ccgaggcttt tgcgcatcaa tatggtttag gaaacaaaaa tggaatcgat ccctacgtaa
     1201 aatcggcagt tttggctaat gaattgggat taggaatcaa tgctggacac gatttgagtt
     1261 tggataatat ccagtttttt catcaaaata ttcccggatt acttgaagtc tccattggtc
     1321 acgcattaat tgccgaatcg ctgtatctcg gattagaaaa tgtagtgaat atgtatttgc
     1381 ataagctaaa ataaattttg ccacgaattc acgaattaaa atttgtgata atttgtgaaa
     1441 tttgtggttg aaaaaacatc aaaaaatgat ttattcaaaa atagaaggaa caggtaaacc
     1501 actcctaatt atacacggat ttcttggaat gtccgacaat tggaaaacta taggaacaca
     1561 atttgcagcc gatggatttc aagtgcattt attggattta cgcaatcacg gcaagagttt
     1621 tcattctgat gaattcaatt atgagataat ggctcaagat gtttttgact attgcaatgc
     1681 caataatcta gaaaaaataa gtattcttgg acattcgatg ggtggaaaaa ctgccatgct
     1741 ttttgctgca aactatccag aaatggtcga aaaattggta gttgccgaca ttggtccaaa
     1801 gttttatccg caacatcatc aaattatttt tgaagccttg aatgcggtag atttttcact
     1861 aaaaccaagt cgtgccgaag tcgaagaaat tatgtctcac tacataaccg attttggaac
     1921 aagacaattt ctgttaaaaa gtttgtattg gaaagagtca gggcaattgg cttttcgatt
     1981 taatttggca gttttcaatc taaacaggga cgaaattgga aaaccacttc ctgaggatgc
     2041 ggttttcaac aaaccaacac tttttattcg cggcggcaat tcgaattata ttttggacga
     2101 tgattttcca aaaatccaac aacattttcc agattctaga atagaaacga ttccaaatgt
     2161 tgggcattgg cttcacgccg aaaaccccaa gatgttttac gaaatgacga gtttgttttt
     2221 gaaataatta ttttatacat ttgaaataaa aaaattatga agctaattct taggattttt
     2281 atcacagcgg ttttagtggt gcttattgca ggagcaatga catcagtaga tgttgacagt
     2341 tttactacag ctgtaattgt tgcggccgtg ttgggattgc ttaatatttt tatcaaacct
     2401 attttagtaa ttttgtcatt gccagctaca atactaacat ttggtttgtt tttatttgta
     2461 ataaatgggg gtataataat gttatgttca agattagttg acggattcag gataaattca
     2521 ttttggacgg ctattatttt tagtataatt ctttccattt gtgagtctat tgtttataaa
     2581 attgtaggcg aagacaaata aatcacagta tattttgttc caaataaggg acttaaattc
     2641 aatagattaa aaacttgttt gggttgtaaa atttttataa ttttgcaacc caattttatt
     2701 atgtaaatta acgaagaaga tggatattaa aagagtagca ttagacgcag taaacgaaac
     2761 aattgtaatg acagttgttc atatggatta caaaggtcaa gtagccaaaa gaataaacga
     2821 aaaaatgcct ttggcacaag taaaaggttt tagaaaagga gccgtgccta aagaccttgt
     2881 tgaaaaacaa tacggaaaag aaatcaaaaa agaagagatt aagaaagtgg ttgatttggc
     2941 tttagagcgt tacatccagt ctgaaagatt gaatcttttg ggaactccac ttcctaaagt
     3001 aaacgaaaac ttggattggt ctgccgaaga attaactttt gaattcgaaa ttggtttggt
     3061 tccatctttc gaaattgatt tggatgctaa aaacgacatc attaaatatg tggttactgc
     3121 cgacgataaa ttgattgacg gtcaagtggc tcgtatccaa aaacaatttg gaactgcaat
     3181 tcctcaggat gttgtaactg aaggtgctga tttgaaaggt atttttacca acgaagccga
     3241 aggaattgga aatacgacta ccattgcatt agctactttc aaagataaag caacccaaga
     3301 tttattcatt ggaaaaaaag taggtgatgt agttacggta aacacaagcg gtttattcga
     3361 agacgatcac caattgatgg attatttgaa agtaggtcac gataacgtac acggtttagc
     3421 aatcgatgtt gatttcacaa tcgaagcaat cactgtttct gaaccagcag aattgaacca
     3481 agaattgttc gacaaattgt ttggtgctgg aaatgtagct tcattagaag acttgaaagc
     3541 aaaaatcaaa gaagatgccg agtctcaatt tgctcaacaa gcagaccaaa aattattagg
     3601 tgatgtaact gagtttttaa tcgaaaacac caaattcgat ttgccagctg aattccttaa
     3661 aaaatggttg caaactgtag gtgagaaaaa attatctcca gaagaagctg aagtagaata
     3721 tgcacgttct gaaaaaggat tgcgttttca attgatcgaa ggaaaagcat tggcgcaatc
     3781 tgacatcaaa atcactttcg aagatttgaa aacttttaca gccaaaaaca tccgtcagca
     3841 aatggcacaa ttcggtcaaa cgaatcctac tgacgaagaa gttcaaggaa tcgtggcaag
     3901 agttttgtcg aaccaagaag aagtaaaaag actttcagac caagtggttg cagagaaatt
     3961 gttggaaatt ttcaaagaaa aagccaatcc aacaactaaa gaagtgactt acgaagaatt
     4021 tattgctgcg tcttacggag agtaatttct aaaaaaaata agtatatttg agcgtcaaaa
     4081 gaatattttt tttgacgctc ttttttttaa agttcttgta aaacttggaa ttaagacata
     4141 ttggcattat ttgtaaagag gtatgacctt tgataaatta aattacgata tataaactaa
     4201 aacttagaac aaataaatat gaactacggt aaagaattta aaaaatttgc aacaaaacac
     4261 caaggtgtaa acgcaatgta ctacgacaaa atcgtggcag caatgactcc aacgaacatg
     4321 actccttata ttattgaaga acgtcagttg aatatttccc aattggatgt tttttcaaga
     4381 ttgatgatgg atcgaattat tttcttggga acaggaattg acgatcaaat tgccaacatc
     4441 gttcaagcgc agttattgtt tttggaaagt gccgatgcct caaaagacat tcaaatttac
     4501 ttgaattctc caggaggaag cgtgtatgca ggattgggaa tttatgacac gatgcagtac
     4561 atcaagccag acgttgccac aatttgcacc ggaatggcgg cttcgatggg agcagtattg
     4621 ttgtgtgctg gagcagcagg aaaacgttcg gctttgccac attcaagagt aatgattcac
     4681 caaccatcgg gtggagcaca aggagtggca tcggatatgg aaatcaactt gcgcgaaatg
     4741 ttgaaattaa aagacgaatt gtataaaatt atttcgcatc attcaggcaa gccttttgac
     4801 aaagtatatg ccgatagcga gcgcgattat tggatgattg ccgatgaagc caaagaatac
     4861 ggaatgattg atgaagtgtt aagaagagga taaatagtta ttggtttatg gttgatagtt
     4921 gatggttttt aatccaacaa ctatcaacca tcaactacca acaaaaaaga aatggcaaaa
     4981 ccaaaattag aatgttcatt ctgtggaaga aaaaaaccgg aaaccaattt attgattgcc
     5041 ggaatcgatg cgcacatttg tgataaatgt atcgaacaag cccacggaat tgtagtagaa
     5101 gaattaaaat caagtagcaa aaatcatttg ccaaccgact tggtgttgag aaaacctaaa
     5161 gaaattaggg cttttctgga tcaatatgtt attggtcagg atcaaaccaa aaaagtaatg
     5221 gcggttgcgg tttacaatca ctacaaacgt ttgatgcaac aagaattgca cgacgaagta
     5281 gagattgaaa aaagcaacat cattatggta ggtcaaaccg gaacagggaa aactttggtg
     5341 gccaaaacca ttgccaaaat gctcgatgtt cctttggcga ttgtcgatgc gactgttttg
     5401 accgaggcag gttatgtggg agaagatgtc gaaagtattt tgacacgact tttgcaagcc
     5461 gccgattatg acgtgaccaa agccgaaaga ggcatcgttt tcattgacga aattgacaaa
     5521 attgcccgca aaagcgacaa tccatccatc actcgagatg tttcgggcga aggcgtgcaa
     5581 caagctttgt tgaaattatt agaaggaacg gttgtaaatg tgccaccaaa aggtggacga
     5641 aaacatccag accaaaaatt tgttgaggtg aatactcaaa atattttgtt tattgctggt
     5701 ggtgcttttg atggtgtgga aagaattatt tccaaaagac taaaccgtca agctgttggt
     5761 tattcaactt ccaaaaatgt ggacaatatc gacaaagata atttgttgca atacatcatc
     5821 ccgaaagata tcaaggactt tggattaatt cctgaaatca ttggtcgttt gccggtgttg
     5881 acgcatatgg atcctttgga tagggaaacc ttgagggcga ttttgaccca gcccaaaaat
     5941 gccttgatca aacaatacca aaagttgttt ttgatggacg aggtggaatt cagcatcacg
     6001 gacgaagcct tggattttgt tgtagataaa gctttggaat ataaattagg agctcgtgga
     6061 ttgcgttctt tatgcgaagc tatcttgacc gatgcgatgt atgaattgcc aagttctgat
     6121 gataaaatat tagaaattga caaagattat gcagaacata ctttgaataa aaatttattg
     6181 aaaagaatgg agattgcttc gtaaattttt gatttataga atttcaaacc cgataggatt
     6241 taaaaaccta tcgggttttt tgtttttttg ctttgtgaaa aaatattcaa tatccttttt
     6301 tatctacaat agattgtcga tttttgccca tcttaaatta atacaataca aatggcacta
     6361 gttgagaaag agattatttt attgaaagtt tcaggccagg ataaacctgg ggtaacggct
     6421 gggttgactt ctatattagc taattatgac gcagttattt tagatattgg tcaagctgat
     6481 attcacgata cattatcttt aggtattttg tttgaaataa aagcaggttc tagttcggca
     6541 gcagttttaa aagacttatt gttcagagcg tatgaattgg gtattaatgt gaagtttatt
     6601 ccaatatcta ttccagatta tgaaatttgg gtaaaaggtc agcgcaaaca acgctatatt
     6661 atcaatgtat tgggcgaaac tttgacagca gcacaactag cggctgtgac tcaaattatg
     6721 tcaaaccaaa aattgaatat cgattctatc attcgattaa cgggtagggt ttcggttgta
     6781 gaaaaagaag aatatccgcg ttcttgtgtg cagttgtctg tgactggaaa tataaatgat
     6841 aaaacgctaa tgactgccag ttttatggaa atttcgaagg aattaaatgt agatatttct
     6901 ttccaagaag acaatatgta cagaagaaac cgacgtttgg tgtgttttga tatggattca
     6961 accttaattc aaaccgaagt aatcgatgaa ttagccgagt tggcaggagt aggcgaacaa
     7021 gtaagagcta ttactgaatc agccatgaat ggcgagattg attttagcga aagcttcaaa
     7081 aaacgcatgg ctttgcttga agggttgagt gaagacgttt tgcacaatgt tgccataaat
     7141 cttccgataa ccaaaggagc ccatcgtttg atgaaagcct tgaaatatta tggttataaa
     7201 acggctattt tatcaggagg atttacctat tttggcgatt atttgcaaaa agaattagga
     7261 attgattatg tctatgccaa ccagttggag ataaaagacg gtaaactgac cggaaaatat
     7321 ttaggtgaaa ttgttgatgg tcaaaagaaa gccgaatatt tgaaagccat tgccgaaaaa
     7381 gaaggcatca atatcaacca aaccattgca gtgggtgatg gtgccaatga tttgccaatg
     7441 ttgaatttgg cgggattagg aattgctttt cacgctaaac ccaaagtaaa agaaagcgca
     7501 gcaacatcca tttctagttt aggtcttgat ggagttttat atctattagg ttaccacgac
     7561 cgacatattg atatgatgca agagtaactt tatagtcaca aagtcacaaa aaaaagcctt
     7621 agattcattg tgaactaagg ctttttttgc ggtaaaacta ttgattttga aaagctatga
     7681 gtttttcaaa ttgataattt cttgttcggt aagcaatctc cagtttccac gaggcaagtt
     7741 cttcttagtc aaacctgcaa aagcaactct gtcaattctc aaaacatcgt agctgaaatg
     7801 ttcgaaaata gaacgaacta ccttcacgtt agacgatctt aattttagac caatttcgct
     7861 tttagcctca ccttcaatat aacttacttc ctctacaaaa acacggtgtc cgtctagaac
     7921 caatcctttt tgtatttttt ctaaatcttc aaatttcaag tttttgtcta aagaaacttg
     7981 gtatattttg gatgatttat tggttggcaa agtaaactta cgaatcatat ccgtatcgtt
     8041 ggtaaacaat aaaagaccag ttgtgttttt atccattctt ccaacagcgc caattttggc
     8101 agtggtggaa ccttttacca attccaaaac attgcggtat tcctgacctt cgtcaagtgc
     8161 tgttgtgaag ttttttggtt tgttcaacaa gatatatact tttttctctg gagttaattt
     8221 taccccatca aaattaacgt catcgcttgg ttttactaga taacccattt cggttactgg
     8281 aattccgttt acttttacgt ttccagattg aatataaata tccgcatcac gacgcgaaca
     8341 cactcccgaa ttggcaatgt atttattcaa acgaatttcg tctttctctt tttgtctctt
     8401 tggagcctga tttggttttt tagcagtttt tgccgcttcc gcctccgcta tttttactgc
     8461 tggttttact tttttaggtc cttgtgctct ttttggcatc gcaggttttg gcttattaga
     8521 gcttggtctg gagctgtttg ctctaccgcc gcttttttta ttgttgcctt ccttgttatt
     8581 cataatatgc tataatttgc tgcaaagata gtttattatt gcttaattgt tgattgtttt
     8641 tgaattaggg tttgcaggag gtgatgggaa cagttcaaca agacagtttt cttgagattt
     8701 atttttaaag aaaaaatgag gttagtataa gattcgtttt attccgtttt atctttttgt
     8761 ttatttcgtg tttcttggtc tttattaatt ttctcaatga cgtccatcga aataacattt
     8821 aactcgtcgg ttttgcaatt ccagatgttt aaatattttt ttgaaacaac attatgggtt
     8881 ttccattttg taaatttttc ataaagcatc aaagtacaaa tgtcggtagc ggtgatgaaa
     8941 tctccggttt ctggcaaatg tgaatgaatt gttccttcaa tttcttttcc ttcttcggac
     9001 tttcctccat attcaattgg aataatattt ttgtgcaatt gtttttttac catcaattta
     9061 ttgtctttgt tgaacgtcaa taagtagtcg ttaccaaaaa tgacaacacc attgtttttt
     9121 ggtcctgtca agacatacac tttcttttcc tttccgttga taataggaat taaattcaaa
     9181 ctggagttgt tatagttctt aaacaaagtg tccgattgga tttctactaa tgcaagtttt
     9241 ctaatttcat agaggtcatt ttcagtagtt gtaaaatttc tttctgttaa attagtgttt
     9301 gctgttttgg tattgtaggt agagtcaaat gatattgttc caattacttt tgggttttct
     9361 gatttagaaa aaaagataca ttttgccaca tcattgtcgg tataagaaaa atagccgcca
     9421 atattttctc tgtttttgta attttctaaa aacaaatctg tgccatacca agaggccatt
     9481 tctgatttat aaagtaattt gccttctgca acaatgggtt gagcttcttt tgttgggttt
     9541 ttttgtccga aaacacaaaa gcttagcaag gtcaatagta atggtaatac tttgttcatt
     9601 tgtatggtgt tgttttagag ttatggttaa cggaagtcta ggagaaaact tcggattaat
     9661 cagtaatcaa atataataaa agaattgctc taaaaacaag atttttcgta ttttattgga
     9721 ttcaaaaaga gctaaccttt tgtaaacata aaatgaagca agattaaatc gaccgtaaca
     9781 atttctctcc gtgccaaagt acgctcggat tgataagtat gatgcagaaa acgcccgcca
     9841 cgatgaggaa tttcaagacg ttgtgaagta aaatatactg ttctttggtg ttagatttcc
     9901 atagacgcag caggaaaaaa atcaaaataa tgaaagaagc atagaagtaa atatccatat
     9961 atcccacgtc aaaaatttcg attaaaacat aaaccggaat gattgtcgag atggtgagaa
    10021 tagtgattat ttttttagaa gtttcttcgc cataaagtat tggaatcgtg ttgtagtcat
    10081 tggccaaatc gcctttgata ttttctaaat ctttgatcat ctcccgaatt aaaatcaata
    10141 aaaacaagaa aacggcgtgt ccaaaaatga cttcatagaa atttttgtaa tacaaaagaa
    10201 tggcaaaaaa aggtaaaatt gccaagaaag cggcggtcaa attgcctata atcgggtact
    10261 ttttgatgcg atgcgaataa taccaaatca aaaaaatata aaccgaaaaa tataaaaaag
    10321 cacgccaaga aacaataata gccaataaag cgacaatgaa attgagggta aaatacacct
    10381 gaagttttgt tttttggctt accaaacgat ccagcatcga ctttgtggga cggttgatta
    10441 agtctttttt actatcataa aaattattga tgatataacc cgatgcaata gtcaaagaag
    10501 aagcaaaaac gatgatgaac aagtcaaaat cgaataggat agacaaggcc cttttttcgg
    10561 gtgcgaggat gaaaatggcc gaaagatatt gcgccagaac aattatcgga atgttgtacc
    10621 ctctaactac agagaacaaa ctggcaattt tcattaaaag aatcttggac tttctgttca
    10681 ttttctattt tggttggcaa cggaaaaacg ctaggtatgt tttttcctcc cctctccttt
    10741 gcagagaggc ttgggggtga ggagatttag aattggtaaa cgacttccag tttatactct
    10801 ttcaaggcgc gttttgcttt ttctaagtct tgtgtaaaac ctaggatata accaccgccg
    10861 ccagaaccgc aaagttttaa ataataatcg ttagagtcaa ttccttcttg ccaaatgccg
    10921 tgaaactgtt ctggaatcat tggtttgaag tgattcaaaa ctactttgga taattttttg
    10981 gtgttggcaa acaacgattt catatcgcct cccaagaaat tttcgacaca agcatcggta
    11041 tgtttgataa attgcaattt caacatagta cggaaacctt ggtctttcaa tttttccata
    11101 aaaatattca ccatcggagc ggtttcgccc acaattcctg aatctattaa aaacacagcg
    11161 ccgtttcctt gtaaactttg agttggaatt ccagtagcct caatatggtc tttagaattg
    11221 attaaaatag gaatgctcaa ataactgttt aaaggatcta atccagaact ttttccgtgg
    11281 aaaaaagatt ccatttgccc gaaaatagtt ttgagttgca ataacttttc acgagttaga
    11341 ttttctagaa cggtaatttt attttgggcg tatttgtcgt aaatggctgc caccaaggca
    11401 ccgctgcttc ccactccgta accttgcgga atgctggaat cgaagtacat t
//
LOCUS       DAOSNN010000054        11418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5662_length_11418_cov_4.652293, whole genome shotgun sequence.
ACCESSION   DAOSNN010000054 DAOSNN010000000
VERSION     DAOSNN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11418
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5662_length_11418_cov_4.652293"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLQ70_04315"
     CDS             complement(<1..261)
                     /locus_tag="PLQ70_04315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQE33873.1"
                     /translation="MQTSKVSVIVPCYNQAQYLDESLASIYHQTHTNWECLIVNDGSL
                     DHTEEIAQKWKAKDPRFIYIPKENSGVSNARNLGIEKASGEFI"
     gene            complement(278..1162)
                     /locus_tag="PLQ70_04320"
     CDS             complement(278..1162)
                     /locus_tag="PLQ70_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HQE33874.1"
                     /translation="MNKLIHSILNKLGYKISNIGKKEKAIQNFVSSFGVKNDTYNLVY
                     NSASYLFEIKKHFPELVLEGHKEGVLASFNGLQVYVESSEEFFIIKEVFIEKDYNLLS
                     NENFVVFDIGMNIGISSLFFALQKNVTQIYSFEPVVPTYNQAVYNRNLNPSCAAKIEA
                     FNFGLGGSTRVEKVLYNAQAKGNCGIRLDASLVLDKNNAAEIEIQIKNIGEILPDLLA
                     KHANQKKVLKIDCEGAEYEILEKLSQLNLLADVDVLLIEWHDKGATLLEELLVANNFS
                     VISRHLTSITGMIYAFKN"
     gene            complement(1209..2078)
                     /locus_tag="PLQ70_04325"
     CDS             complement(1209..2078)
                     /locus_tag="PLQ70_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE33875.1"
                     /translation="MNPTHTNENDWLFEITPKNKFFSLNLKEVWQYRDLLMLFVKRDV
                     VTVYKQTILGPLWYLIQPLFTSVTFTIIFNTVAGINTGTVPPFLFNLAGITVWNYFTA
                     CLTGTSDTFKANAAIFGKVYFPRIITPMSVVISNLIKFGIQFLIFIGFYVFYYFQGAP
                     LSLNSSLLFFPLLILLMGTLGLGLGMFISSLVTKYRDFSYLIGFGVQLLMYLSAVMYP
                     MALIQSKMPDYAWLVHYNPLAYIIETTRYMLLGIGAVSVWGMVYTVSVTLIVFFMGLL
                     VFNKTEKSFIDTV"
     gene            2298..3032
                     /locus_tag="PLQ70_04330"
     CDS             2298..3032
                     /locus_tag="PLQ70_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HQE33876.1"
                     /translation="MFTIFQSKPILKDIIPDNYIDIHSHILPGIDDGAKTLGDSLQLI
                     QALQGFGASKLITTPHVIQHFWENSSQNIKEKEAETNLELKKNNLSIPFRAAAEYMMD
                     ENFVKLFQSEKLLTLKSNYVLVEMSYINPPIQLYSILFDLQVAGYIPVLAHPERYNFF
                     HKNFEEYNKLINAGCLLQLNLLSVVGYYGESITEIAQKLLKKGMYSFVGSDAHHNNHI
                     ASFDKKVKIKDLAPLKEVIANNQFFI"
     gene            complement(3247..>4149)
                     /locus_tag="PLQ70_04335"
     CDS             complement(3247..>4149)
                     /locus_tag="PLQ70_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HQE33877.1"
                     /translation="YSIAKLYKITLSDLQKANPDLGKKSLKVGQKISIPGDIETNLIA
                     ASDTKEAKTQTESSVLTPAVVEKKGQDANPTNPAAIPSTVENLKEVVAYTHEVLPKET
                     KYGIAKQYGLSVVELEKQNPAIAKNLLVGSKLKILSSKSIEKTASMQTAIAIETEYIK
                     EKFETTNTNITHDEAFVDQLISRASENIGTRYRSGGTTKEGFDCSGLMCYTFSESDIK
                     LPRSSIEMASYGSKIDTQDAQKGDLIFFKTSGRGRITHVGMVVEVLDGEIKFIHSSNH
                     GGVIISSTKESYYERSLVQINRVL"
     assembly_gap    4150..4249
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4250..4890)
                     /locus_tag="PLQ70_04340"
     CDS             complement(<4250..4890)
                     /locus_tag="PLQ70_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQE33878.1"
                     /translation="MKYFSCLFLIVFFSSMSLFSQEKLRQHTVAKGETISEIAKKYQV
                     EQSAIYELNPDAVNGIKLKSVLLIPTKAKKSNSISVRDSARDSSEKTHEVLPKETLYG
                     ITKQYKVSLEDLYKSNPNLEKEGLKTGQVLKLPQTALVDFPENKIAEKTIEVPKTNLS
                     QKKNLKEEPAVVEIKNNNKIELPTNTIQHEVLPKESLYSIAKLYKITLSDLQKA"
     gene            5181..6524
                     /locus_tag="PLQ70_04345"
     CDS             5181..6524
                     /locus_tag="PLQ70_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQE33879.1"
                     /translation="MKKIRLLIFLIPLFSFTQNIKGIVLSEKENLPIKDVNITLKSFS
                     VNSKTDDNGEFKLRVPVELAETDSISFTHVGYASKTIVFSELKKNNFLIYLEEKIESL
                     PGISIQANKPLKSKIAFTKQKPSKYPLFSFGSILKDNKIYVIGGDASFATDAWKKVQY
                     EKVDPTMEDYLRELSFQFSGQYYKGNLLIYDIKTNTWETSKLKFRKRAYHNLNEYNNK
                     IYVLGGKRVSANGVFEYLDDKIEVFDPENQSIIIDNTNPHQAADFASFEYNGNLIVMG
                     GSTKMNEDAVKLYSNKVHSYNINSGYWYELDNLPTAKETTGVLFKNKIYLVGGFNSKP
                     LNTIESFDLITEKWTTEGELFKAVSRPAITNDGNLIYFFDNERIYTLDTNTRLLKEYQ
                     IDLPLQASKLFYSEDKLYLFGGYIENYYSKYPSPNLISIDINEFKNTKPIRAKFL"
     gene            complement(6596..9367)
                     /locus_tag="PLQ70_04350"
     CDS             complement(6596..9367)
                     /locus_tag="PLQ70_04350"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HQE33880.1"
                     /translation="MSIYNDYLNEIEERKGQGLHPKPIDGAELLSEIIAQIKDVNNAY
                     RADSLQFFIYNTVPGTTPAANVKAKFLKEIVLGESVVKEITVAFAFELLSHMKGGPSI
                     EVLLDLALGSDVAIAKQAAEVLKTQVYLYDADMARLGDAFKSGNEIAKEILESYAKAE
                     FFTKLPEVAEEIKIVTFIAGEGDISTDLLSPGNQAHSRSDRELHGKCMITPEAQAEIK
                     ALQAQHPDKSVMLIAEKGTMGVGSSRMSGVNNVALWSGKQASPYIPFVNIAPIVGGTN
                     GISPIFLTTVDVTGGIGLDLKNWVKKVDEAGNVLRDESGDPILEEVYSVATGTVLTIN
                     TKTKKLYKGDQELIDISKAFTPQKLEFIKAGGSYAIVFGKKLQTFASKTLGIDTVPVF
                     APSKEVSIEGQGLTAVEKIFNKNAVGTTPGKILHAGSDVRVTVNIVGSQDTTGLMTSQ
                     ELESMAATVISPIVDGAYQSGCHTASVWDNKSKANIPRLMKFMNDFGLITARDPKGVY
                     HAMTDVIHKVLNDITVDDWAIIIGGDSHTRMSKGVAFGADSGTVALALATGEASMPIP
                     ESVKVTFKGTMKSYMDFRDVVHATQSQMLQQFGGENVFQGKIIEVHIGTLTADQAFTF
                     TDWTAEMKAKASICISEDATLIESLEIAKSRIQIMIDKGMDNKNQVLQGLINKADKRI
                     AEIQSGEKPALKPDANAKYYAEVVVDLDLIAEPMIADPDVNNADVSKRYTHDTIRPLS
                     FYGGDKKVDLGFIGSCMVHKGDMKILAQMLENIEEQNGKVEFKAPLVVAPPTYNIVDE
                     LKAEGDWEVLQKYSGFEFDDSAPKGTARTSYENMLYLERPGCNLCMGNQEKASKGDTV
                     MATSTRLFQGRVVEDSAEKKGESLLSSTPVVVLSTVLGRTPTIEEYKTAVEGINLTKF
                     APSHKLLVK"
     gene            complement(9703..10656)
                     /locus_tag="PLQ70_04355"
     CDS             complement(9703..10656)
                     /locus_tag="PLQ70_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQE33881.1"
                     /translation="MSDVAAIHNLVQKRNSLKAEIAKIIVGQDTVVDQILLCIFSGGH
                     ALLVGVPGLAKTLMVNTLAQALGLDFKRIQFTPDLMPSDILGSEILDENRHFKFIRGP
                     IFSNIILADEINRTPPKTQAALLEAMQERSVTIAGHNYKLDLPYFVLATQNPIEQEGT
                     YPLPEAQLDRFMFAIKLDYPSFEEEVQVVKRTTSDSHSVINPLFTSQEIIEFQHLIRR
                     IPVADNVIEYAVTLVSKTRPDNPLSNEFVKNYLDWGAGPRASQNLILAAKANAAFNGK
                     FSPDIEDVKAVATGILRHRIIKNYKADAEGITEEMIIDKLI"
     gene            complement(10729..>11418)
                     /locus_tag="PLQ70_04360"
     CDS             complement(10729..>11418)
                     /locus_tag="PLQ70_04360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQE33882.1"
                     /translation="SLVYACAGLGFYKMTKKTPFVKEFKEVAFSLQEGEISEPFETDF
                     GYHIIYVEKIKGQEVELRHILLIPSVTPEDLKEAKEKITLIKKRIEDKEITFDKAAKT
                     MSDEKETRANGGALINPKTQDTRFELTKMDPTLYSRVSNLKEGEITSPIMDDDQSGKK
                     KYKIITVTNRINEHTADYAKDYIKIKDLALKEKQIKAIGKWFDDKIKDTYIRVNGEYR
                     DCTFTNNWLKK"
BASE COUNT         3701 a         2128 c         1728 g         3761 t
ORIGIN      
        1 aataaattcg ccactagcct tctcgattcc cagatttcta gcgttgctaa ccccactgtt
       61 ttctttaggg atataaataa atcgggggtc tttggctttc catttttgag cgatttcttc
      121 ggtatgatcc aagctgccat cattgacaat tagacactcc caattagtat gcgtttgatg
      181 gtagatggat gccaaagatt cgtccaaata ttgggcttga ttgtagcagg gaacaataac
      241 agagactttt gaagtttgca tcgcttagtt cttgttttta atttttaaag gcataaatca
      301 tcccagtaat agaagtcaga tgtctcgaaa ttacactaaa attattagcg accaatagct
      361 cttccaaaag tgtagctcct ttgtcatgcc attcgattag caatacatca acatcggcta
      421 ataaattcag ttggcttaat ttttctaaaa tctcatattc tgcgccttcg caatctattt
      481 ttaatacttt tttctgatta gcatgtttcg ccaacaaatc aggaagaatc tcgccaatat
      541 tctttatctg aatttcaatt tctgcagcat tatttttatc caaaacaagg cttgcatcca
      601 atcggatacc gcaatttcct ttagcttgtg cattatacaa tactttttca actcgagtgg
      661 aaccacccaa tccaaaatta aaagcttcta tttttgcagc acaactaggg tttaaattcc
      721 tattgtaaac cgcttgatta taggtaggaa cgacaggttc aaaagaatag atttgggtta
      781 catttttttg caaggcaaaa aacaaagaac taatcccgat attcatccca atatcaaaaa
      841 caacgaagtt ttcattagac aataaattat aatctttttc gataaaaact tctttaataa
      901 taaaaaactc ttcggagctt tctacataaa cttgcaatcc attgaaacta gctaaaacac
      961 cttctttatg cccttctaaa acgagttctg gaaaatgttt ttttatttca aataaatatg
     1021 aagcagaatt ataaactaga ttataggtgt cattctttac cccaaaactg cttacaaaat
     1081 tttgtatcgc tttttctttc ttgccaatat tcgagatttt gtatcccagt ttatttaaaa
     1141 tgctatgaat tagtttgttc attatttata ttttgtttcg ttctgcgtcc tgccttctga
     1201 attctgcact aaaccgtatc tataaaactt ttctctgtct tattgaagac caaaagtccc
     1261 ataaaaaaaa caataagtgt cacactcact gtataaacca ttccccaaac agaaacagct
     1321 cctattccta ataacatata acgagtagtt tctataatat aagccaaggg attgtagtga
     1381 actagccaag cataatcagg cattttgctt tgtatcagcg ccataggata catcaccgcc
     1441 gaaagataca tcaacagttg cacaccaaat ccaatgaggt aactaaaatc tcggtatttg
     1501 gttaccaaag acgaaataaa catccccaat cccaatccca aagtccccat caacaaaatg
     1561 agcaagggaa aaaacaagag cgaactattc aaacttagcg gagctccctg aaaataataa
     1621 aatacataaa agccaatgaa aatcagaaat tgaattccaa acttaattaa attagaaatc
     1681 actaccgaca ttggtgttat tatcctagga aaatagactt tgccaaaaat agcggcattg
     1741 gccttaaaag tatctgaagt acctgtcaaa caagcggtaa aataattcca aactgttatt
     1801 cccgccaaat taaataaaaa cggaggtacc gttccagtat taattcctgc tactgtatta
     1861 aaaatgatgg taaaagtaac tgaagtaaac aaaggctgaa tcaaatacca caaagggcct
     1921 aaaatggttt gtttataaac cgttacgaca tctcttttta caaaaagcat aagcaaatct
     1981 cggtattgcc aaacttcctt gaggttgagt gagaagaact tgtttttggg tgttatttca
     2041 aacagccaat cattttcatt agtatgggta ggattcatag taagtacaaa attcaatcta
     2101 aaacaaatct gaaatgagcc atttatatct caacgctttt gtcctattgc tttgtcgcaa
     2161 atataataat ttaattgaac tcttggtagt tgtttgttgt tagttgctgg ttgctcgaaa
     2221 acggtggacg attgactaat gacgaaaaac gacaatgcga gtaaccaaat aaaacccaaa
     2281 ttttgtatct ttgcactatg tttacaatat tccaatccaa gcccattcta aaagatataa
     2341 ttcctgataa ttatattgat attcattccc atattttacc cggaattgac gatggcgcca
     2401 aaactttagg cgatagttta caattaatac aagcgcttca aggatttggt gcttcaaaat
     2461 taattacaac cccacacgtc attcagcatt tttgggaaaa cagcagccaa aatattaaag
     2521 aaaaagaagc agagaccaac ttagagctca agaaaaacaa cctttcaatt ccttttcgag
     2581 ccgcagcaga atatatgatg gacgaaaatt ttgtaaaact ttttcaatcc gaaaaactgt
     2641 tgacccttaa aagcaattat gttttagtcg aaatgtccta tatcaatcct cctatccaac
     2701 tctattctat tttgtttgac cttcaggtgg caggttatat tcccgttttg gcacatccag
     2761 aacgatacaa tttttttcat aaaaatttcg aagaatacaa caaactgatt aatgcaggct
     2821 gtttattgca attaaaccta ttatccgttg tgggttatta tggcgaaagc attaccgaaa
     2881 tagcccaaaa attactcaaa aaaggaatgt acagctttgt agggtctgat gcacatcaca
     2941 acaaccacat tgcctctttt gacaaaaaag taaaaataaa agatttagct cctttgaaag
     3001 aagtgattgc aaacaatcaa ttttttatat aaatttctgc aaaatgtacc acgcattttt
     3061 tgcccgagat ttcaagaaag tggcgtgact ttgacaaagt gctaactttt aatcacgaag
     3121 tttttttgcc acagattgca cagattccca cagatttttt gaatcaggaa agatatagca
     3181 tagcttatgt aaagattttt tataaaaaaa ctagttaaac aattaaacag ttaacccatt
     3241 aaacctttac aacacccgat taatttgaac caaacttctc tcgtaatagg actctttggt
     3301 tgaagaaata ataacacctc cgtgattaga ggaatgaata aatttaattt caccatctaa
     3361 cacttcaacg accataccca cgtgagtaat tcggccacgg ccacttgttt tgaaaaaaat
     3421 taaatcccct ttttgagcat cttgagtgtc aatcttagaa ccataagaag ccatttctat
     3481 agaagatctt gggagtttaa tatcgctttc actaaaagta taacacatca atcccgaaca
     3541 atcaaaacct tccttagtag ttcctcctga acgatatcga gtcccaatat tctcagaagc
     3601 tctcgaaatc aattgatcta caaatgcttc atcatgagtt atattagtat ttgttgtctc
     3661 gaacttttct ttgatatatt cagtttcaat agcgatagct gtctgcatag aagccgtttt
     3721 ttcaatactt ttcgaactaa gaatctttag cttagaaccc accaataaat ttttggctat
     3781 agcaggattc tgtttttcta attcaaccac cgatagcccg tactgtttgg caatcccata
     3841 cttggtttcc tttggcaaaa cttcgtgtgt gtatgcgaca acttctttta ggttttcaac
     3901 tgtagatgga attgcagctg gatttgtagg attagcatct tgcccttttt tctccacaac
     3961 cgcaggagtc aaaacagaac tttccgtttg cgttttagct tcttttgtat cggaagcagc
     4021 aatcaaattg gtttcaatat ctcctggaat actaatcttt tgacccactt ttaaggattt
     4081 ctttcctaaa tcaggattcg ctttttgcaa atcagaaagg gtaattttat aaagcttggc
     4141 tatagaatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ctttttgcaa
     4261 atcagaaagg gtaattttat aaagcttggc tatagaatat aatgattctt taggcaaaac
     4321 ttcatgttga attgtattag ttggaagctc aattttatta ttgtttttta tttcaacaac
     4381 ggcgggttct tcttttaaat ttttcttttg agaaagattt gttttaggaa cttcaatagt
     4441 tttttcagct atcttatttt ctggaaaatc tactaaagcg gtctggggaa gttttaggac
     4501 ttgacctgtt ttcaatcctt ctttttctaa attaggatta cttttataca agtcttcaag
     4561 actcactttg tattgcttgg taattccgta aagggtttcc ttgggcagta cttcgtgtgt
     4621 tttttcagaa gaatcacgag cactatctcg aaccgaaatc gaattgctct ttttggcctt
     4681 agtaggaatt aataaaaccg attttaattt aattccattt acagcatcag gattcagttc
     4741 ataaatagcc gattgttcaa cttggtattt cttagcaatt tcactgattg tttcaccctt
     4801 agctacggta tgttgcctta acttttcttg cgaaaaaaga ctcatgctac taaagaaaac
     4861 aatcaaaaaa aggcaactaa aatacttcat aaacactatt taaattcaat atttatcgca
     4921 ttcattcttc aattaacaac gcgatacaac aacctcaatt attctgaaaa ttgaataatt
     4981 attttacttt tacggcaaac tttaacaata aagtataacc taaaaacaaa tttaagctat
     5041 aaatataaat ttcgcaattt ttaacccgct ctttataatc aatttaacaa agttttgatt
     5101 gaaaattagc aaaattatag ccctacgatt aaaaaatata tagtaatttt aatcctccaa
     5161 cagacctaat gaaatctttt ttgaaaaaaa tacgattact tatatttctc attcctctat
     5221 tttcatttac acaaaacata aaagggattg tattaagtga gaaggaaaat ctacctatta
     5281 aggatgtaaa cataactcta aaaagtttta gcgtgaattc taaaacggat gataatggcg
     5341 aattcaaact tagagttcca gtagaattgg ccgaaactga ttctatctca tttacacatg
     5401 ttggatatgc ctcaaaaact attgtattct cagaattaaa gaagaataat tttttgattt
     5461 acctagaaga aaaaatcgaa tccttacctg gaattagcat tcaagccaac aaaccattaa
     5521 aatcaaaaat tgcttttaca aaacaaaaac cttcaaaata ccccttgttt tcttttggtt
     5581 caattctaaa agacaacaaa atctatgtca ttggaggaga tgcctcgttc gcaacagatg
     5641 cttggaaaaa agtacaatat gaaaaagtag atcctacaat ggaagattat ctgcgtgaac
     5701 ttagttttca gttttctggt caatactata aaggaaacct tcttatttat gatataaaaa
     5761 ccaatacttg ggaaacttct aaactaaaat ttagaaaaag agcctatcat aatctaaacg
     5821 aatacaataa taaaatttat gttttaggag gaaaacgggt ttctgcaaat ggagtttttg
     5881 aatatttaga cgataaaatt gaagtttttg accccgaaaa tcaatctatt attatagaca
     5941 acactaatcc tcatcaagca gctgattttg cctcttttga atataatggc aatctaatag
     6001 tcatgggagg atcaacaaaa atgaatgaag atgcagttaa attatactct aacaaagttc
     6061 actcttacaa tataaattcg ggttattggt atgaattaga caatttaccc acagctaaag
     6121 aaaccaccgg agtcttattt aaaaacaaaa tatatttagt cggaggcttt aattctaagc
     6181 ctttgaacac catagaaagt tttgacttaa ttaccgaaaa atggacaact gaaggagaat
     6241 tatttaaagc ggtaagcagg ccagccatta caaatgatgg caatttgatt tatttttttg
     6301 ataatgaaag aatctacact ttagacacaa acacccgatt gttgaaagaa taccaaatcg
     6361 atttgccttt acaagcttca aaattatttt attcagaaga caaattgtat ttatttggtg
     6421 gttatatcga aaactactat tctaaatatc cttcgccaaa tttaatcagt attgatatca
     6481 atgaatttaa aaacacgaaa ccaattaggg cgaaattctt ataaatcctc tatttaaaac
     6541 ataaaaaaag cctgagatat gactcaggct tttaaatatt tataatactg tatgattatt
     6601 tcactaacaa cttatgcgat ggagcaaatt tagttagatt gataccttca acagccgttt
     6661 tgtattcttc tattgttgga gttcgtccaa gtacagtaga taaaactaca actggagtag
     6721 aagataataa tgattctcct tttttctctg cagagtcttc tacaacacga ccttggaaca
     6781 aacgagttga tgttgccatt accgtatctc ctttagacgc tttttcttgg ttccccatac
     6841 aaaggttaca acctggacgc tctaaataca acatattttc atacgaagta cgagcagttc
     6901 ctttaggcgc actatcatca aattcgaaac cagagtattt ttgtaacact tcccagtcgc
     6961 cttcagcttt caactcatca acaatgttat aagttggtgg tgctactaca agcggtgctt
     7021 tgaactcaac tttaccattt tgctcttcta tattttcaag catttgagct aagattttca
     7081 tatcaccttt gtgaaccata caagaaccta taaatccaag atctactttt ttatctccac
     7141 cataaaaaga taaaggtcta atggtatcgt gagtatatct tttagaaaca tcggcattat
     7201 ttacatcagg atcggcaatc attggctctg caatcaaatc caaatcaacc acaacttcag
     7261 cataatattt cgcattagca tctggtttta aggctggttt ttctcctgat tgaatttctg
     7321 caattctctt atctgctttg ttgattaagc cttgaagcac ttggtttttg ttatccatgc
     7381 ctttgtcaat cataatttgg attctgcttt ttgcaatttc caatgattca atcaaagtag
     7441 catcttcaga aatacagata gacgcttttg ctttcatctc agcagtccag tctgtaaatg
     7501 taaaggcctg gtcagcagta agcgtcccga tgtgaacctc gatgattttt ccttgaaata
     7561 cgttttcgcc accaaactgt tgcaacattt gagattgagt agcatgcacc acatcacgga
     7621 aatccatata cgatttcatt gttcctttga acgttacttt cacagattct ggaattggca
     7681 tagaagcctc tcctgtagct aatgccaaag caacagtacc tgaatcggca ccaaaagcaa
     7741 ccccttttga cattctagta tgtgagtcac caccgataat gatagcccaa tcatcaaccg
     7801 taatgtcatt caatactttg tgaattacat cggtcatagc gtggtaaacg cctttggggt
     7861 cacgagctgt aatcaaaccg aaatcgttca taaatttcat caatctagga atattcgctt
     7921 tcgatttatt atcccaaacc gaagcagtat gacatcctga ttggtaagca ccatcaacga
     7981 ttggcgaaat aactgtagcg gccatagact ctaattcttg tgaagtcatc aaaccagtgg
     8041 tatcttgcga acctacaatg tttacggtta cacgaacgtc agaaccagcg tgcaaaattt
     8101 tacccggagt tgttccaact gcatttttat taaatatttt ttcaactgct gtaagtcctt
     8161 gtccttcgat agaaacttct tttgacggag caaatacagg tacagtatca atacctaaag
     8221 ttttagaagc gaatgtttgc agtttttttc caaatacaat agcataagaa ccacctgctt
     8281 ttataaattc aagtttttga ggagtgaatg ctttagaaat atctattaat tcttggtctc
     8341 ctttgtatag ttttttggtt ttagtattga tagtaagtac cgttcctgtt gcaacagaat
     8401 atacttcttc caaaatagga tcaccacttt cgtcacgaag aacattccct gcttcatcca
     8461 ccttttttac ccagttttta agatccaaac cgataccacc agttacatca acagtcgtta
     8521 agaaaatagg agaaattccg ttagtaccac ccacaattgg agcaatattc acgaatggaa
     8581 tgtatggact ggcttgttta ccgctccaaa gtgccacgtt atttacacct gacatccttg
     8641 aagaacccac tcccattgtt cctttttcag caattaacat caccgatttg tcagggtgtt
     8701 gtgcttgtaa tgctttgatt tccgcttgag cttcaggcgt aatcatacat tttccgtgca
     8761 actcacgatc agaacgagag tgtgcttggt ttcctggaga aagcaaatcg gttgaaatat
     8821 ctccttcacc agcaataaaa gtaactattt taatttcttc agctacctct ggaagtttag
     8881 taaaaaactc cgctttagca taactttcca aaatttcttt tgcaatttcg tttccgcttt
     8941 tgaatgcgtc gcctaaacga gccatatcag catcataaag atatacttgt gtcttaagaa
     9001 cttctgccgc ttgttttgca atagccacat cgctacccaa agccaaatca agcaatactt
     9061 caatagaagg tccgcctttc atatgtgata ataattcaaa ggcaaaagcc actgtaattt
     9121 cttttaccac ggattctcca agaacaatct ccttcaaaaa tttagctttc acattagctg
     9181 caggagtagt tccaggaaca gtattgtaaa taaaaaattg aagcgaatct gctcgataag
     9241 cattattcac atctttaatt tgagcaatga tttcgcttaa taattcagca ccatcgattg
     9301 gtttaggatg aagtccttga ccttttcgct cttcgatttc gtttagataa tcgttataaa
     9361 tactcataat agaagtcttt tcagtgttaa aggcaaattt ctttgatgaa ttcaaaaaca
     9421 aatgccaaaa tagtttttgg tttttatatt ttttttgtga tgcaaaatta agaatttatg
     9481 ctgagattta aaaaatatta gcataataag ccgtttcctt gctgataatt acacataaaa
     9541 gaaattaaat gtcttttatt gaaaaattct aaaaatcaga ttgaaaattt aaataatcgc
     9601 tagtttagag catataaaaa aagacttata aatcactttt agagctttaa aaccctgaat
     9661 agtttttttc cagctaaata aaatgaaata tactgaaatg aattaaatca atttatcaat
     9721 aatcatttct tcagtaattc cctcagcatc ggctttatag tttttgatga ttctgtgacg
     9781 aagaattcct gtagccaccg cttttacatc ttcaatatcg ggagaaaatt ttccgttgaa
     9841 tgctgcattg gctttagccg ccaaaatcaa attttgagaa gctctgggtc ctgctcccca
     9901 atctaaataa tttttgacaa attcattaga caaaggatta tctggacgtg ttttactgac
     9961 caaagtaact gcatattcaa ttacattatc ggccactgga atcctacgaa tcaagtgttg
    10021 gaattcaatt atttcttgag aagtaaacaa aggatttatt actgaatgcg aatcggaagt
    10081 tgttcttttt actacctgaa cttcttcttc aaaagaagga taatctagtt tgatagcaaa
    10141 cataaaacga tctaattgcg cttctggcaa aggataagtt ccttcttgct caattggatt
    10201 ttgagtagcc aatacaaaat aaggcaaatc tagcttatag ttatgaccag caatggttac
    10261 tgaacgttcc tgcatcgctt caagcaaagc tgcctgcgtt tttggaggcg ttctattgat
    10321 ttcgtcggct aggataatat tcgaaaatat ggggcctctg ataaatttga aatggcgatt
    10381 ttcatccaat atttcacttc ctaaaatatc agatggcatt aaatcgggcg taaattggat
    10441 tcttttaaaa tctaaaccta atgcttgtgc taaggtattt accatcaaag tttttgctaa
    10501 tcctggcact cctactaaaa gtgcgtgacc tccagaaaaa atacacaata gaatttgatc
    10561 tactaccgta tcttgaccca caataatttt ggcgatttcg gcttttagcg aattcctttt
    10621 ttgaactaaa ttatgaatag ctgcgacgtc tgacatattt ttgaatgatg agttatgaat
    10681 tatgaatgat gagttttcat ataaaaactc atcattcata acttataatt attttttgag
    10741 ccaattgttt gtaaaagtac aatctctgta ttcaccgtta actctaatat aagtgtcttt
    10801 aattttatca tcaaaccatt ttccgatagc tttgatttgt ttttctttta aagccaaatc
    10861 tttaatttta atataatctt tggcataatc agcagtatgc tcgtttattc tattggtaac
    10921 cgtaattatc ttgtattttt tctttccgga ttgatcatca tccattatgg gcgaagtgat
    10981 ttctccttct ttcaaatttg aaactcgact gtacaaagta ggatccattt tggtcaattc
    11041 aaaacgagtg tcttgagttt taggattaat taaagcgcct ccatttgctc tagtctcttt
    11101 ttcgtccgac atggtttttg ccgctttatc aaaagtaatt tccttgtctt cgattctttt
    11161 tttaattaaa gtaatttttt ctttagcctc ttttaaatcc tctggcgtaa cagaaggtat
    11221 caataatata tgacgcaatt ctacctcttg acctttgatt ttctctacat aaataatgtg
    11281 ataaccaaaa tccgtttcaa agggttcaga aatttcacct tcttgaagac taaatgctac
    11341 ctctttaaac tctttgacga aaggtgtttt tttggtcatt ttataaaaac ccaggcccgc
    11401 acaggcatac accagcga
//
LOCUS       DAOSNN010000055        11346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5713_length_11346_cov_4.376140, whole genome shotgun sequence.
ACCESSION   DAOSNN010000055 DAOSNN010000000
VERSION     DAOSNN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11346
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5713_length_11346_cov_4.376140"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..874)
                     /locus_tag="PLQ70_04365"
     CDS             complement(212..874)
                     /locus_tag="PLQ70_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE33883.1"
                     /translation="MIHAKNIHKYYDQLHVLKGVDLHIQKGEIVSIVGASGAGKTTLL
                     QILGTLDKPSIENGIELSINGQDILTMSDKILSKFRNLNLGFIFQFHQLLPEFTALEN
                     VCIPAFIANKSKQETEIEAKKLLEYLGLSHRINHKPNELSGGEQQRVAVARALINKPA
                     VIFADEPSGNLDTHSAENLHQLFFKLRDEFGQTFVIVTHNEELANMADRKLVMVDGLI
                     SK"
     gene            1009..1302
                     /locus_tag="PLQ70_04370"
     CDS             1009..1302
                     /locus_tag="PLQ70_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33884.1"
                     /translation="MAEEVIVEKESYKNRNLILGIVFDAIGMLSFSIPLLGEFSDVVW
                     APIAGFMMTWMYKGTVGKMGGIFTFLEEIIPFTDFIPSFTLTWIYNYWIKKEK"
     gene            complement(1312..1617)
                     /locus_tag="PLQ70_04375"
     CDS             complement(1312..1617)
                     /locus_tag="PLQ70_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglyceryl transferase"
                     /protein_id="HQE33885.1"
                     /translation="MENLKKRWGIESNFQFVIIFVVFAINGSLSARISGFAMKYLGIN
                     NENTHWIPYYLILILLVLPLYPFLLMFFGWLFGQSKFFFPFSKKMLKSMGFGFIFKN"
     gene            complement(1650..2126)
                     /locus_tag="PLQ70_04380"
     CDS             complement(1650..2126)
                     /locus_tag="PLQ70_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6146 family protein"
                     /protein_id="HQE33886.1"
                     /translation="MKNYFYILALTLFLIVSCNSNKTTVSNSEKNLTFKNDTIQVAKN
                     TVKIKNDTIRIANDSLEYEVIIIDPGFSTWLASMAQPRNFHSLTYLETKNQIYVNEWN
                     NRVMQPQRYNPNLYEMRINYEPFIHYGYEVNYLIYNYMIYFQNTFKQKLDGYVPIR"
     gene            complement(2240..2797)
                     /locus_tag="PLQ70_04385"
     CDS             complement(2240..2797)
                     /locus_tag="PLQ70_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33887.1"
                     /translation="MFEQLTQLVQQYGNEVVVKNNAIPNEHNEAVMTEAGGSILSGLQ
                     DMVAGGNFGDLAGMLSGKSPIDMNNPVVKELAGKVTDNLGSKFGISSETASGVADGLI
                     PQVLSGLVNKAQDPNQPEFNVSDLIGSISGGNSAGLMDAVSKYGGQFGLDQDGDGQVG
                     MSDAIAAVSKNSAGFGGLLSKLFGK"
     gene            complement(2892..3842)
                     /locus_tag="PLQ70_04390"
     CDS             complement(2892..3842)
                     /locus_tag="PLQ70_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HQE33888.1"
                     /translation="MKVLANDGISESGILALQKGGFEVITTKVAQEQVANFVNENNVS
                     VVLVRSATKVRKDIIDACPGLKIIGRGGVGMDNIDVDYAKSKGIHVINTPASSSESVA
                     ELVFAHLLSGVRFLHDSNRNMPLEGDSNFNGLKKAYADGVELRGKTLGIVGIGRIGQA
                     TAKMALGLGMKVIATDGFIPQVDVKVEFFDGQSITTTIVTQSLESLFQEADFITLHVP
                     AQDGYIVDEAALATMKDGVGIVNCARGGVIDEVALIKALDSGKVSFAGLDVFESEPKP
                     EMAILMHPKISLTPHIGAATGEAQDRIGTELASQIISILG"
     gene            complement(3872..4942)
                     /gene="serC"
                     /locus_tag="PLQ70_04395"
     CDS             complement(3872..4942)
                     /gene="serC"
                     /locus_tag="PLQ70_04395"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HQE33889.1"
                     /translation="MKKHNYSAGPSILPQEVFEKSAQAILDFNNSGLSILEISHRSKD
                     FVAVMDEARALALELLGLEGKGYQALFLAGGASMEFLMAPYNLMKENGKAAYLDSGTW
                     ATAAIKEAKFFGETVIVGSSKEDNYTYVPKGYEIPSDADYFHCTSNNTIFGTQMKEFP
                     TTTVPVVCDMSSDIFSRVLDFSKFDIIYAGAQKNMGPAGTTLVVIKESILGKNGRVIP
                     SMLDYAKHIKAESMYNTPPVFPVYASLLTMKWIKEKGGVAAVEKLNDAKAALLYGEID
                     RNPLFKGAAVVEDRSTMNATFLLTNPEHTETFDKMWKAAGISGLPGHRSVGGYRASIY
                     NAMPIESVQVLVDVMQALEKAV"
     gene            complement(5036..6094)
                     /locus_tag="PLQ70_04400"
     CDS             complement(5036..6094)
                     /locus_tag="PLQ70_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HQE33890.1"
                     /translation="MAQNDKKRVFIELGKFLSQFSEQENRKDSTVLFNDLFFEDFENL
                     ILLSQSHNGWYTPENVYFAIQSWASALSEANLEKWLSGYSIEINEPKNIGLILAGNIP
                     LVGFHDFLSVLISGNNVLIKTSSNDQYLLPFLAKYIIAIEPELANKITFVEGKLENFD
                     AVIATGSNNTARYFEYYFKDKPSIIRKNRNSVAVLNGEETKEQLVALGEDIFRYFGLG
                     CRNVSKLFVPKGYSFDAFFEAIFKYQDIIHYEKYANNYDYNKAVFLMSNFKLLDNGFL
                     TIKEDNSYASPISSVFYEYYDSIEDLQTRLQSENEQIQCIVSNKLVENSIDFGNTQRP
                     NLWDYADNVDTISFLLTI"
     gene            6224..6571
                     /locus_tag="PLQ70_04405"
     CDS             6224..6571
                     /locus_tag="PLQ70_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQE33891.1"
                     /translation="MAIIITDECINCGACEPECPNTAIYEGADDWRYKDGTRIKGKVV
                     LPSGEEVDADAAQTPISDDIYYIVPGKCTECKGFHKEPQCAAVCPVDCCIPDENHVES
                     EETLLARQAFLHN"
     gene            complement(6698..7126)
                     /locus_tag="PLQ70_04410"
     CDS             complement(6698..7126)
                     /locus_tag="PLQ70_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HQE33892.1"
                     /translation="MKKIVLFFLTLLLVSCGASSPKKSDSNSLYEVLTNQADGGASIR
                     FFEILSDEKEIAMLQNDEKLKNKIKPNDIQTSNFIVLNMGEKTSGGYKISIESAVETD
                     KNIIITLKETAPESGSNASQEITTPYCVVKINSKKEIIIK"
     gene            complement(7123..8256)
                     /gene="bshA"
                     /locus_tag="PLQ70_04415"
     CDS             complement(7123..8256)
                     /gene="bshA"
                     /locus_tag="PLQ70_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HQE33893.1"
                     /translation="MKIAIVCYPTFGGSGVVATELGLELARRGHEIHFITYSQPVRLA
                     LLNPNVHYHEVNVPEYPLFHFQPYELALSSKLVDMVKLYKIELLHVHYAIPHAYAGYM
                     AKQMLKDEGINLPMITTLHGTDITLVGNHPFYKTAVTFSINKSDFVTSVSQSLKEDTL
                     KLFNIKNEIQVIPNFIELDKHETEAITSCRRSVMATEKERIITHISNFRKVKRIPDVI
                     QIFYKIQQEIPAKLMMVGDGPEKEKAEYLCQELGILDRVIFFGNSNEIDKILSYTDLF
                     LLPSETESFGLAALEAMAWSVPVISSNSGGLPEVNFDGISGYLSNKGNTDEMAQNALK
                     ILKDDATLNKFKKNALSVAKQFDIENILPLYEELYHKAINKFS"
     gene            complement(8308..8790)
                     /locus_tag="PLQ70_04420"
     CDS             complement(8308..8790)
                     /locus_tag="PLQ70_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HQE33894.1"
                     /translation="MYVPFENLPEDSKIWIYQSNRKFSDAEFLEIETDVKSFLDSWEA
                     HSVSLESSYQLKYNRFIIIAVNQEVQAATGCSIDKSVQFIQKLEQKYNVDLLDKMNVT
                     FKNGEHIAHKSLIDFKKMAKEKAVTENTIVFNNLVNNIEEFNESWEVPAMDSWHSRFF
                     "
     gene            complement(8831..9622)
                     /locus_tag="PLQ70_04425"
     CDS             complement(8831..9622)
                     /locus_tag="PLQ70_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQE33895.1"
                     /translation="MSEVLIVPTMAEMLAQGKQPEVLFWVGCSGSFDDRAKKITKAFI
                     RILNRANVSFAVLGTEESCSGDPAKRAGNEFLFQMQAMMNIEVLNAYEVKKIVTACPH
                     CFNTLKNEYPELGGVYEVVHHTEFLKSLLDDDRLTIEGGQFKGKRITFHDPCYLGRAN
                     NVYEAPRDLIQKLDAELVEMKRSRANGLCCGAGGAQMFKDAEAGNKEINIERTEDALE
                     TKPEIIAAGCPFCNTMMTDGIKNKEKESEVKVLDIAELIANAQDL"
     gene            complement(9695..11029)
                     /locus_tag="PLQ70_04430"
     CDS             complement(9695..11029)
                     /locus_tag="PLQ70_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQE33896.1"
                     /translation="MGFLSTILFAIFLVLGFGYFYFNIKKIVRNINLGTAVNRKDNPK
                     ARWKNMAMIALGQSKMVKRPVAGILHIIVYAGFIIINIELLEIIIDGLFGTHRIFAFL
                     GTSYNVLIGSFEILAFLVLVSVFTFWIRRNIIRLKRFANKDLTGFPKNDANYILYFEV
                     VLMTLFLLMNASDLHLQNIPGGFSHFIKAGFFPISQFIEPLFNGVSSNLVFILNEMFW
                     WLHIIGILIFMNYLYYSKHLHILLAFPNTYFADLNPKGQLSNLESVTNEVKLMMDPNA
                     DPYATPVLDENAVPSKFGASDIQDLNWVQLMNAYTCTECGRCTSSCPANITGKKLSPR
                     KIMMDTRDRIEEVGKNIDANNGVFVPDNKSLLNDYITPEELWACTSCNACVEECPVNI
                     SPLSIIVDLRRYLVMEQSAAPTSLNAMMTNIENNGAPWQYNQQDRLNWKNEI"
     gene            complement(11043..>11346)
                     /locus_tag="PLQ70_04435"
     CDS             complement(11043..>11346)
                     /locus_tag="PLQ70_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HQE33897.1"
                     /translation="SNFSKISDSLNKADLGKTAANLQKTLEKVDKIMADLQAGKGSMG
                     KLMKDEALYNNLEQTSKELELLLEDVRLHPTRYVNVSVFGKKNKPYVAPVKDTISK"
BASE COUNT         3652 a         2018 c         1782 g         3894 t
ORIGIN      
        1 tgattaacca gagaaaaaga tatttaagga tttacaatcg aagtgtaagt tgtgcattca
       61 atattcattt aaaaaatagc aacaattgta attatagaaa aatataacca caaacttaga
      121 attgcaagta agaaacaatt taactgtgat tcctactaaa aatgggatta cgctagaata
      181 atccaactca atacaattaa aatcataatt attattttga aatcaatcca tccaccataa
      241 ctaattttct atctgccata ttagctaatt cttcgttgtg ggttacaatt acgaaagttt
      301 gtccaaattc gtcacgaagt ttaaaaaaca attggtgcag attttcggct gaatgcgtat
      361 cgagatttcc cgatggttcg tcggcaaaaa taacagcagg cttgttgatt aaagctcgtg
      421 caacggctac acgctgttgt tctccacccg aaagttcgtt gggtttgtga tttattcgat
      481 gagacaaacc aagatattcc aataattttt tagcttcgat ttctgtttct tgtttggatt
      541 tattggcaat gaatgcagga atacaaacat tttctaaagc ggtaaattca ggtaataatt
      601 gatgaaattg gaaaataaaa cccaaattca aatttctgaa tttagataaa attttgtcgc
      661 tcattgtcaa aatatcttga ccattgatgc tcaattctat tccgttttca atagagggtt
      721 tatccaatgt tcctagaatt tgcaaaagcg tagtttttcc agctcccgaa gcacctacga
      781 tagaaacgat ttctcctttt tgaatgtgta aatcaactcc tttaaggacg tgaagttggt
      841 cataatattt atgtatgttt tttgcatgta tcattattgg gaacagtttt cacaaagaaa
      901 caaagatttt agctgatttc taaaattatc tttcgacttt aaggttaaat aaaatctaaa
      961 attatttttg gcattggatt tgtatctttg ttttgaaaat ttattaaaat ggcagaagaa
     1021 gttatagtag aaaaagaaag ttataaaaac agaaatctta ttttaggaat tgtattcgat
     1081 gcaattggaa tgctatcttt ttcaattcct ttacttggag aattctcgga tgtggtttgg
     1141 gctccaattg cagggtttat gatgacttgg atgtataaag gaactgttgg aaaaatgggt
     1201 ggtattttta cttttctaga agaaatcatt ccgtttacag atttcattcc ttcttttacc
     1261 ttgacttgga tttacaatta ttggataaaa aaagaaaaat aattaatgct cttaattttt
     1321 aaaaataaac ccaaaaccca tactttttag catttttttc gaaaaaggaa agaaaaattt
     1381 agactgacca aaaagccatc cgaaaaacat taacaaaaaa ggatacaagg gtaaaaccaa
     1441 aagaattaga attaaataat acggaatcca atgggtgttt tcattattaa tgcccagata
     1501 tttcatcgca aaaccagaaa ttctagccga taaagaaccg tttatggcaa aaaccacaaa
     1561 aataattaca aactgaaaat tggattcaat tccccagcgt tttttcaaat tctccatatt
     1621 cattctaaaa aataacaaat atagtgagat tatctaattg ggacataacc atctagtttt
     1681 tgtttaaagg tgttttgaaa ataaatcata taattgtaaa ttaagtagtt cacttcatat
     1741 ccataatgga taaatggctc atagttgatt ctcatttcat acaaattagg attatatcgt
     1801 tgcggttgca ttacacgatt gttccattca ttgacataaa tttggttttt ggtttccaaa
     1861 taagttagcg aatgaaaatt tcgaggttga gccatacttg caagccaagt actaaatccg
     1921 ggatctataa taataacttc atattctaga gaatcattgg caattcttat ggtgtcattc
     1981 ttaattttta cagtgttttt tgcaacttga atcgtatcgt ttttgaatgt caggtttttt
     2041 tctgaattag aaacagtcgt tttattgcta ttgcaactta caatcaaaaa aagagtcaaa
     2101 gctagtatgt agaaataatt tttcattttg aaaaagtaaa tgataaagtt acaaaaacat
     2161 aagattgaat cgtaattttt agataaaaaa agtcgtttgc tttctacaaa cgactttttt
     2221 aaaaaggtat attcaattat tatttcccaa aaagtttgct taataacccg ccaaatccag
     2281 cactattttt actcaccgca gcaatagcat cactcattcc tacttgtcca tctccatctt
     2341 gatctagacc aaattgtcca ccgtattttg aaacggcatc catcaaacct gcactatttc
     2401 cgccagaaat agaaccaatt aaatcagaaa cattaaattc aggttgattt gggtcttgtg
     2461 ctttgtttac taatccgctt aatacctgag gaatcaatcc atcagcaaca ccactagccg
     2521 tttctgaaga tattccaaat ttagacccta aattatcagt tacttttcca gctaattctt
     2581 ttacaacagg attgttcata tctattggag attttccaga gagcattcca gccaaatcgc
     2641 caaaatttcc acctgcaacc atatcttgta aacccgaaag aatggagcct ccagcttcag
     2701 tcattactgc ttcattgtgt tcatttggaa tagcattatt tttcacaact acttcatttc
     2761 cgtattgttg tactaattgc gttaattgtt caaacatagt ttgtaaattt aaaattgaat
     2821 ccaaatctaa taaaaaaagc tcactattaa aataaattaa taatgagctt tatatagtta
     2881 acttaaaatt attatcctaa aatactaata atttgtgacg ctaattcagt tccaattctg
     2941 tcttgagctt ctcctgttgc agctccaatg tgaggagtca acgaaatttt tgggtgcatc
     3001 aaaatagcca tttctggttt tggttcgctt tcgaaaacgt ctaagcctgc aaaagatact
     3061 tttcctgaat ccaatgcttt gattaaagca acttcatcaa taacaccacc acgagcgcag
     3121 tttacaattc caacaccgtc tttcatagtt gctaatgctg cttcgtccac gatataacca
     3181 tcttgagcag gaacgtgcaa tgtgatgaaa tcagcttctt gaaacaacga ttccaatgat
     3241 tgagtaacga ttgttgttgt aatagattgt ccatcgaaaa attctacttt tacatctact
     3301 tgtggaataa atccatcagt agcgataact ttcattccta gaccaagagc catttttgct
     3361 gtagcttgac caatacgacc aattccaaca attccaagtg tttttcctct taattcaact
     3421 ccatcagcat atgctttttt caaaccatta aagtttgaat ctccttcaag aggcatattt
     3481 ctgttagaat cgtgcaagaa acgaacgcca gaaagcaagt gagcaaaaac caattcagct
     3541 accgattctg atgaagaagc aggcgtgttg attacgtgaa ttcctttgct tttagcataa
     3601 tcaacatcga tgttgtccat tccaacacca ccacgaccaa taattttcaa tcctggacaa
     3661 gcgtcgatta tatctttgcg aactttggtt gcacttctaa ctaaaactac gcttacgttg
     3721 ttttcgttta cgaaattagc cacttgttct tgagctactt ttgttgttat tacttcaaat
     3781 ccaccttttt gtaaggctag aattccactt tctgaaattc cgtcattggc taatactttc
     3841 atttattttt tttaattaaa agattaaatt tttaaactgc tttttccaaa gcttgcatta
     3901 catctactaa aacttgcacg ctttcaattg gcattgcgtt ataaatagaa gctctgtaac
     3961 cacctactga acggtgtcct ggcaatccag aaattccagc tgctttccac attttgtcaa
     4021 atgtttcggt gtgttctgga tttgtcaata aaaaagtagc attcatagta gaacgatctt
     4081 ctactactgc agcccctttg aataatgggt ttctgtcaat ttctccgtat aataaagccg
     4141 ctttagcatc gttcaatttt tcaactgctg ctacaccacc tttttctttg atccatttca
     4201 ttgttaataa tgaagcgtaa acaggaaaaa caggaggagt attgtacata ctttctgctt
     4261 tgatatgttt tgcatagtct aacatacttg gaataactct tccgttttta ccaagaatcg
     4321 attctttgat aactaccaaa gtagtcccag caggtcccat atttttttga gcaccagcat
     4381 aaataatatc gaatttagaa aaatctaaaa ctcttgaaaa gatatcagaa ctcatatcgc
     4441 aaactacagg aacagtagtc gttggaaatt ccttcatttg agttccaaaa atggtattgt
     4501 tactggtaca gtgaaaataa tcggcatcac ttggtatttc gtaaccttta ggaacataag
     4561 tataattatc ttctttggaa gaacctacaa taacagtttc tccaaaaaac ttagcttctt
     4621 ttatagcagc agttgcccaa gttcctgaat caagataagc agcttttccg ttttctttca
     4681 tcaagttata aggtgccatc aaaaattcca tactagctcc gccagcaaga aaaagagctt
     4741 gatatccttt gccttctaat cctaaaagtt ctaatgctaa tgcacgtgcc tcgtccataa
     4801 cggcaacgaa atctttactt cggtgcgaaa tttctaaaat agacaatcct gaattattga
     4861 aatctaaaat agcttgtgct gatttttcaa aaacttcttg tggtaaaata gatggtcctg
     4921 cgctgtagtt gtgttttttc atggtataat ttatgtccaa aaaattaagt tgcaaatttc
     4981 ggaaatcaga gttgaataag cattaaataa ttcgaaataa ttcgcttatt tttttctata
     5041 ttgttaacaa aaacgatatt gtatcaacat tatctgcata atcccataaa tttggccttt
     5101 gagtgttgcc aaagtcaatg ctattttcga ctaacttatt acttacaata cactgaattt
     5161 gttcgttttc agattgtagc cgagtttgta aatcttcgat tgaatcatag tattcgtaaa
     5221 aaacactaga aataggtgaa gcataactat tgtcttcttt aatagtcaaa aatccattgt
     5281 ccaataattt gaaattactc atcaagaaaa cggccttgtt gtaatcataa ttattagcat
     5341 atttttcata gtgaatgatg tcttggtatt tgaaaatggc ctcgaaaaaa gcatcaaaag
     5401 aatagccttt tggcacaaaa agtttcgaaa cattgcggca tcccaatcca aaatacctaa
     5461 aaatatcttc tcccaaagca acgagttgtt cctttgtttc ctctccgttt aaaacagcaa
     5521 ctgaatttct gttttttcga ataatacttg gtttgtcttt aaaataatat tcaaaataac
     5581 gtgctgtatt attgcttcca gtggctataa cggcatcaaa attctctaat tttccttcga
     5641 cgaaagtgat tttgtttgcc aattcaggtt caattgcaat gatatatttt gctaaaaaag
     5701 gcaataaata ttggtcattt gaagaagtct ttatcaatac attattccca gaaatcaata
     5761 ccgataaaaa atcgtgaaat ccaaccaacg gaatatttcc agccaagatt aagccaatgt
     5821 ttttgggttc gtttatttcg attgaatagc ccgagagcca tttctctaga tttgcttccg
     5881 aaagagctga tgcccaagat tggattgcaa aatagacgtt ttctggagtg taccagccat
     5941 tatgtgattg tgaaagtaga attaaatttt caaaatcttc aaaaaacaaa tcattaaaca
     6001 aaactgtaga gtcttttctg ttttcttgct cggaaaattg gcttaaaaat tttcctaatt
     6061 caataaatac tcttttttta tcgttttgtg ccatttctaa tttgtttatg aaaagttttg
     6121 attgtaattt tgtgcaaaaa taaggataat tatttcgaaa gtcttcaagt aaaagcgaaa
     6181 ttgaccttag tttatgatta tgaaacttta aaccaaaaat agtatggcga ttattataac
     6241 cgatgaatgt atcaattgtg gtgcctgtga accagaatgt ccaaatactg caatttacga
     6301 aggagcagat gattggagat ataaagatgg tacaagaatt aaaggaaaag tggttcttcc
     6361 ttctggagaa gaagtggatg ctgatgctgc tcaaactcca atttctgacg atatttatta
     6421 tattgttcct ggaaaatgta ctgagtgtaa aggatttcat aaagaacctc aatgtgcggc
     6481 tgtttgtcct gtagattgtt gtattcctga tgaaaatcac gtagaaagcg aagaaacctt
     6541 gttagctaga caagcatttt tgcataacta ataacgatta ttttcacaaa caaataaaaa
     6601 atcctgaatt aagttcagga tttttttatg gttcatagtt taaggtttaa tcttcaaaaa
     6661 agagacagtc tgaaaattaa ttttcgaaca gcctctttta ttttataata atctcctttt
     6721 tagaattaat tttcaccaca caatagggtg ttgtgatttc ctgactagca ttcgaacccg
     6781 attctggcgc agtttctttg agggtaataa ttatattttt atcggtttca acagcacttt
     6841 ctatgctaat tttatatcct ccagaagttt tttcacccat attcaaaaca atgaaatttg
     6901 aagtttgaat atcattaggt ttgattttgt tttttaattt ttcatcattt tgaagcattg
     6961 caatttcctt ttcatcagat aaaatttcaa aaaaacgaat gcttgctcct ccatcagctt
     7021 gattggttaa gacttcatac aaagaattcg aatctgattt tttgggagaa gaggctccgc
     7081 aggaaaccaa aaggagtgtt aaaaagaata aaactatttt tttcatgaaa atttatttat
     7141 cgctttatga tataactctt catataaggg taagatgttt tcgatatcaa attgctttgc
     7201 aacagataac gcattttttt tgaatttatt taaagtagca tcgtctttca atattttcag
     7261 agcattttga gccatttcat ctgtattgcc tttattgctc aaataacctg aaattccatc
     7321 aaaattcact tcgggcaaac ctccagaatt actcgaaatt actggaacgc tccacgccat
     7381 tgcttcgaga gctgcaagtc caaaactttc ggtttcggag ggtaataaga acaagtcggt
     7441 ataactcaat attttatcta tttcattact gtttccaaaa aaaataactc tgtctaaaat
     7501 acccaattct tggcataaat attcggcttt ttctttttct ggtccatcgc ctaccatcat
     7561 taatttagct gggatttctt gttgaatttt ataaaatatt tgaattacat caggaattcg
     7621 ttttaccttt ctgaaattac taatatgagt aataattcgc tccttttctg ttgccatcac
     7681 cgaacgacgg caagaagtaa ttgcttctgt ttcgtgttta tccaattcta taaaattcgg
     7741 aatgacttga atttcatttt taatattgaa taatttaagt gtatcttctt tcaaactttg
     7801 ggaaaccgag gtcacaaaat cggatttatt gatgctgaat gtcactgcag ttttataaaa
     7861 cggatgattg cccaccaaag taatatcggt accgtgaagc gtggtaatca tgggcagatt
     7921 tatcccttcg tctttgagca tttgcttagc catatagccc gcataagcgt gtggaatagc
     7981 ataatgaaca tgcaataatt ctattttata caactttacc atatcgacta acttgctcga
     8041 taaagccaat tcataaggct gaaaatgaaa caaaggatat tctggcacat tcacttcgtg
     8101 gtaatgcaca ttaggattca gcaatgccaa acgcacgggt tgactatagg ttataaaatg
     8161 aatttcgtga cccctacgag ccaactcaag acctaattct gtagcgacaa cgccacttcc
     8221 tccgaatgtt ggataacaaa caattgctat tttcatgttt tacttataat gaaatgaaat
     8281 agggtaaaga aaaaacttaa tagtttttta gaaaaaacga ctgtgccaac tatccatagc
     8341 tggaacttcc caagattcgt tgaattcttc gatgttgttg accaaattat taaaaacaat
     8401 ggtgttctcg gtaacagcct tttctttagc catttttttg aaatctatca acgatttatg
     8461 cgcaatgtgt tctccgtttt tgaaagtcac attcatttta tccaacaaat ctacattgta
     8521 tttttgttct aatttttgga taaactgcac tgatttatca atggaacaac ctgtggctgc
     8581 ttgtacctct tggtttacag caattataat gaatcgattg tatttcaatt gataagacga
     8641 ttctaggctt acgctgtgtg cttcccaact gtcaagaaaa gattttacat cggtttctat
     8701 ttctaaaaat tctgcgtcag agaattttct gttggattga taaatccaaa ttttggagtc
     8761 ttctggtaaa ttttcgaaag gaacgtacat ttgattttat atttatgatt ttagaattta
     8821 gatttgtgat ttacaaatcc tgtgcgttgg caataagttc agcgatatcc aacactttta
     8881 cttcgctttc tttttctttg tttttgatac cgtcggtcat catcgtgttg caaaatggac
     8941 aacctgcggc aatgatttcg ggtttagttt ctaatgcgtc ttcggttctt tcgatgttga
     9001 tttctttgtt tccagcttct gcatctttaa acatttgcgc tcctcctgct ccgcaacaca
     9061 gtccattggc acgggaacgt ttcatttcga ctaattcggc atccaatttt tgaattaaat
     9121 cacgtggtgc ttcatataca ttgttggctc ttcctaaata acaaggatcg tgaaaagtaa
     9181 tgcgttttcc tttgaattgt cctccttcta tagttaatct gtcatcgtct aacaaggact
     9241 ttaggaattc tgtgtgatga acaacttcat aaacgcctcc caattcaggg tattcgtttt
     9301 tcaacgtatt gaaacaatgc ggacaagccg taacaatttt tttaacttcg taggcattca
     9361 aaacttcgat gttcatcata gcctgcatct gaaacaaaaa ctcgtttcct gctcttttgg
     9421 cgggatctcc agaacaactt tcttcagtac ccaaaactgc aaaagaaaca ttagctcgat
     9481 ttaaaattcg aataaatgct tttgtgattt ttttggctct atcatcaaaa cttcctgaac
     9541 aacccaccca aaacaatact tctggttgtt tgccttgagc cagcatttcg gccattgttg
     9601 gtactattaa aacttctgac atttcttttt tgtttaatca ttgaagcgtc aaatcggtta
     9661 atcggttaaa catttaaacg attaaacaat taaactatat ttcgtttttc caattcaatc
     9721 ggtcttgttg gttgtattgc caaggtgcgc cgttattttc gatattagtc atcattgcgt
     9781 ttaaggacgt tggtgctgca ctttgctcca ttaccaaata gcgacgcaaa tctacaataa
     9841 tcgaaagtgg actaatattt acaggacatt cctcaacaca agcattacag gatgtgcaag
     9901 cccataattc ttcgggggtg atgtaatcgt ttaataacga tttgttgtct ggaacaaaaa
     9961 caccattatt ggcatcgata tttttgccta cttcttcgat tctatctctc gtatccatca
    10021 taattttacg aggcgataac ttcttgccgg tgatattcgc aggacaagat gatgtacaac
    10081 ggccacattc agtacaagtg taggcattca ttaattgtac ccaattcaaa tcctgaatat
    10141 cgctggctcc aaatttgctc ggcacggcat tttcatcaag aactggcgtg gcataaggat
    10201 cggcattagg atccatcatc aattttactt cgttggttac cgattctaaa ttactgagtt
    10261 gtccttttgg gttcaaatct gcaaaatagg tgtttggaaa agccaacaga atatgaaggt
    10321 gtttagaata atataaataa ttcataaata tcaaaatccc aatgatgtgc aaccaccaaa
    10381 acatttcatt caaaataaat actaaattac tagaaacgcc attaaacaaa ggttcaataa
    10441 attgacttat gggaaaaaaa cctgctttaa tgaaatgcga aaaccctcca ggaatatttt
    10501 gcaaatgcaa atcggaagcg ttcattaaca gaaataaagt catcaaaact acttcgaaat
    10561 aaagaatata attggcatcg tttttaggaa aacctgttaa atctttgttg gcaaaacgtt
    10621 taagtctgat tatatttcta cgaatccaaa aagtaaaaac agatactaaa accaagaatg
    10681 ctaaaatttc gaaagaacca attagcacat tgtaggatgt tcctaaaaaa gcaaaaattc
    10741 tatgagttcc aaacaatcca tcgataatga tttctaataa ttcgatgttg atgatgataa
    10801 atccagcata aacaatgata tgaagaattc ctgctactgg tcttttcacc attttggatt
    10861 gaccaagagc aatcattgcc atatttttcc aacgggcttt tgggttgtct ttgcgattta
    10921 ctgcggtacc taaattaata tttcgaacga ttttttttat attgaaataa aaataaccga
    10981 atcctaaaac cagaaatatc gcaaataaaa tagtgcttaa aaaacccata attgctagtt
    11041 ttttactttg aaatggtatc ttttaccggg gcaacataag gtttgttttt ctttccgaaa
    11101 acggatacat tcacataacg agttgggtga agtcttacat cttcaagcaa taattctaat
    11161 tcttttgagg tttgctctaa attgttatac aaagcttcat ctttcattaa tttgcccata
    11221 gaaccttttc cagcttgcaa atcggccatt attttatcta ctttttctaa agttttttgc
    11281 aaattggcgg ctgtttttcc taaatcggct ttgtttaatg aatcggatat tttagaaaaa
    11341 ttgcta
//
LOCUS       DAOSNN010000056        11286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5740_length_11286_cov_4.626213, whole genome shotgun sequence.
ACCESSION   DAOSNN010000056 DAOSNN010000000
VERSION     DAOSNN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5740_length_11286_cov_4.626213"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..756)
                     /locus_tag="PLQ70_04440"
     CDS             complement(58..756)
                     /locus_tag="PLQ70_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQE33898.1"
                     /translation="MKKATAICESCSNENCFIKKHLHLEKMQQYIEKKNSFVCKKSQQ
                     FIIEGAPTQGLYFVYKGKVKTVKTGIYGREQIVRLTTNGDIVGFRGFATSKRCLIGAS
                     ALEDTILCNFSNEDMLDILKNVPEFTFDLMLFYAEELNKSENNIRKIAQMNVRERVID
                     TLLYIHRKFGQSKGVIEVELSRRDIADFAGTTEEQVIRVISSLKKESLLKTVGKKIGL
                     VNVEKMRSEIIEHK"
     gene            918..4430
                     /locus_tag="PLQ70_04445"
     CDS             918..4430
                     /locus_tag="PLQ70_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQE33899.1"
                     /translation="MQNSEIKTTCSYCGVGCGIIVTNDSKNGVEVKGDVNHPVNRGML
                     CSKGMNLHYVVNDTSDRILYPEMRWSKSHPKERVSWDDALERAAAVFSSIIKKHGPDS
                     VGFYVSGQCLTEEYYLINKLVKGFLKTNNVDTNSRLCMSSAVVGYKKTFGEDIVPIAY
                     DDIELADTFLITGANPAWCHPILFRRLEQHKEKNPKTKIIVIDPRRTDSAALADLHLQ
                     IIPGTDIILYHAIGKRLIEKGYIDNDFIKNHTENFNLYKDLVLSSSFEKASKLCGVSV
                     NDIKLAADMIGKAKGFISLWAMGLNQSAIGVDKNTALLNLSLVTGQVGKPGSGPFSLT
                     GQPNAMGGREVGGMANLLAVHKELNNPTHRKEVADFWGVNEISEKPGLTATEMFDALE
                     SGKMKAIWIICTNPLVSLPDSRRVEKALQNAKFVVVQDISHNADTSKFADLLLPAAGW
                     LEKEGTMTNSERRISYLPKGVNAPGEALSDVEILIRFAQKMNFNGFNFNTTEEVYKEY
                     CMMTKDTKMDISFLNYNRLKNEGTFQWPVPDYGHPGTPRLFTDKKFYTPSQKAIFNIP
                     TAIENTSEQPNSQYPFILTTGRIRDQWHTMTKTGKVSRLMTHIPSPMLEINPIDAFKS
                     GINAGDIVVVTSKNGTVRVKAKITDSIREGVVFLPMHWGKQLENDLNRTNNLTNTLVD
                     PTSKEPDFKFTTVSIEKYVKAFEKIAVVGAGAAAFRFIQNYREINTTDEIIVFSNEEN
                     PFYNRVLLPEYMTGEFTWEQLQKIKEEALSKLNITMKSNISIDNINTQEKTILDSQGN
                     LYTFDSLILATGSRPFVPENAQLHLPGRFTMRRKSDADRLKNYLESTNLPAEEQHVVI
                     IGGGLLGLELAAALKHKKVKITIIQRASRLMERQLDRISSKLLAEEVQLRDIQIYFDN
                     EVSTVFETDNPNELEIALKSGRIITANAIVYTIGTIPNIEIAKESGLACGRGVKVNQY
                     LQSSNPSIFAIGEIAEFNNQLFGITSAAEEQADILANFMAGDISSFYKGSVLMNILKL
                     EDINLCSIGQVEVPENDDSYEEIVFADLRQRYYKKCIVKNDLLVGAILMGDKNEFAEF
                     KTLIESKIELADKRNMLLRGSSSAKPVLGKLVCSCSQVGSGNIEEAIKGGVTNFTELC
                     KTTGAGLGCGSCKTEVKDILAKCK"
     gene            4505..5932
                     /locus_tag="PLQ70_04450"
     CDS             4505..5932
                     /locus_tag="PLQ70_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HQE33900.1"
                     /translation="MKNTRLIVKGGVISPGELREIVNMSLEQGLKAISFGSRQDIIFP
                     EDFLPIGKDKTDKYFFVYPDENSGNNIVSSYVSTDIFTNTPWLTGNKFLYILEQFKEQ
                     PQLKVNITDPKQQLVPLFTGHINFVASHHEDYWYLYIRLPNWDKMEVYPVLIYSWNIA
                     EVYYAIEKIQIEEPDTVEMIFQLVNDSLDTNNRTIDKPLQLPFYPFPYYEGMNKLGID
                     QYWLGLYWRNNLYDLDFLKEMCDLCFDCKIGKICITPWKSFIVKGIPKDRKLDWEKFL
                     GKKGINVRHSLLELNWHLPVATDWALNLKTFLVRTLDQFDVSTYGLTFGISEYNREGH
                     YFTSIVVEKNQMPKALETMKIRATYNVLYAKNFDPNTKEYIVLAQDVDKQELPTILIE
                     LSKKYFEELGNTVLEINQPIVKKELQERDIHQCSECLTLYNSEYGDVAQGIQKGVLFK
                     DLPEDYCCSLCESPKNNFINFLEKV"
     gene            5987..6535
                     /locus_tag="PLQ70_04455"
     CDS             5987..6535
                     /locus_tag="PLQ70_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQE33901.1"
                     /translation="MNTKFKTVSSSNISISQLMLPSHTNFSGKIHGGFILSLLDQIAF
                     ACASKFSGNYCVTASVDTVNFLKPIEVGELVTMKAKVNYVGKSSMIVGIRVEAENIQT
                     GEKNHCNSSYFTMVAKDENGNKALVPGLILSNVEEVRRFQNCLKQIALKKQSDAQKNS
                     IDFGSIESIMSSNLYNVKLDLK"
     gene            6897..7274
                     /locus_tag="PLQ70_04460"
     CDS             6897..7274
                     /locus_tag="PLQ70_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQE33902.1"
                     /translation="MKNKITFVLSLLFGLMFINAGLNKFLNYMPMPDNLPEKMQKAMA
                     AFMEIGWIMPLVGIVEILGGLLIIIPKTRALGALMIFPIMVGIVLVNIVQDTSGLPIA
                     AVFSAILLWIMYESKEKYAPLFK"
     gene            7467..7913
                     /locus_tag="PLQ70_04465"
     CDS             7467..7913
                     /locus_tag="PLQ70_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQE33903.1"
                     /translation="MKPTTLIKVLILILTAQKSIAQDSKTATTLTDSKYTIENCVNQF
                     ELEKATKTKVGYQYWFADKNFTLENTLKMSIVEPGKSTHAPHHHIEEEFFYILEGSAS
                     FFLDGKTVVVGPNTSLYCPSNVEHGISNAGQTELKYLVIKKDRKEK"
     gene            complement(7988..8061)
                     /locus_tag="PLQ70_04470"
     tRNA            complement(7988..8061)
                     /locus_tag="PLQ70_04470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8025..8027),aa:Arg,seq:acg)
     gene            complement(8167..8310)
                     /locus_tag="PLQ70_04475"
     CDS             complement(8167..8310)
                     /locus_tag="PLQ70_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HQE33904.1"
                     /translation="MFKILAQINKLILPSFTKRGLNLAKAKKWQLAIIGYRYYVTTRA
                     LDC"
     gene            complement(8341..10239)
                     /locus_tag="PLQ70_04480"
     CDS             complement(8341..10239)
                     /locus_tag="PLQ70_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HQE33905.1"
                     /translation="METLFIFLVKASGLIGIFYLAYILLLRKETFFNSNRWFLLAGLI
                     TSVVLPLVVFTKIVWVEPTPTNFDWSNIPMTTTTVENEAFEINWYLVFAVVYSIGIVG
                     FLVKFALDFYSLSKVLKGKTIQHQADFQFIDVTENIAPFSYFNSIVYNSSLYSETELE
                     NILEHEKVHSTQNHTADVLISRLFCVVFWFNPLMWLYKKAIVQNLEFIADNEASKIIS
                     DKKAYQLTLLKITTQENCVAITNHFYQSLIKKRIVMLNKNQSNKRNSWKYLLVLPLLG
                     AFVFFFQVKVIAQEKEAEQTVVENSKTVENSVDVYKINKNTTDAELKEKIKTLKEKYD
                     VTLAFSGLERNAKNELTAIKVDLKKGKEITKKMATKGTEAIKTFGIIISKNENGKLTV
                     DFGTDEGVINSKNSIASAKETLPIEKEIFIDGGKVSQEDLDKLDSNEIESIDVTKKAD
                     KGTIRIVTKKFSKTLNENDIYINDEKVDKNELLLLDQNTITKMDVNKNEKTIRITTKT
                     IHQSADNIDIPTPPTPPVPPSFTFKTPKPPVFPKAPKSPKGSPTENKEMWKNYEKQIN
                     EYELQVKKMQLEAQNSDENMKAFEQKMKIYEEQMKVYEEQMKVYEEQMGEYGKKMEEL
                     YSKQKTNN"
     gene            complement(10244..10600)
                     /locus_tag="PLQ70_04485"
     CDS             complement(10244..10600)
                     /locus_tag="PLQ70_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HQE33906.1"
                     /translation="MQKLTNKEEEIMQILWKLEKAFVKEVLAEITEEQPHYNTLSTII
                     RNLEEKGFVSHNAFGNTHQYFPIVKMEDYRKRFMNTAIDTYFNSSYKNMVSFFAKEEK
                     ISAEELREILAMIEKK"
     gene            complement(10746..>11286)
                     /locus_tag="PLQ70_04490"
     CDS             complement(10746..>11286)
                     /locus_tag="PLQ70_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE33907.1"
                     /translation="IVMVFFQIFTTLPLYHHEKFGLTEVQTGMLLSLNGLLVFFLEMP
                     IVSQFERKQTNKIKIISLGALCVALGYFVLLLDSWIFILIISIILLSLGEIFAFPFSN
                     AVALNRAPKGQEGQYMGLFTMSFSLAHIVCSKTGLDIIDNYGYQTNWIVMGSLAILAL
                     LCCVWLHRMLQTERLANKI"
BASE COUNT         3687 a         1779 c         2042 g         3778 t
ORIGIN      
        1 ctatacagag aacttagttt ttaagtatta gtcaaaaaaa agcatcgata acgatgctta
       61 tttgtgctct atgatttcgg atctcatttt ttctacattt actaatccta tttttttgcc
      121 taccgtttta aggagcgatt ctttttttaa actcgaaatg actcgaatta cttgttcttc
      181 ggttgtgcca gcaaaatcgg caatgtcacg ccgggataat tctacttcaa ttactccttt
      241 tgattggcca aattttcgat gtatatataa taaggtatca attactcttt ctcgaacatt
      301 catttgggca atttttcgga tattgttttc gcttttgttc aattcttcag cataaaacaa
      361 catcaaatca aaggtgaatt caggcacatt tttcaggata tccagcatat cttcattgct
      421 gaaattgcac aaaatagtat cctcaagtgc tgatgctcca atgaggcaac gtttgctggt
      481 agcaaagcct ctaaacccaa caatgtctcc atttgtagtt agtcttacta tttgctctct
      541 gccataaatg ccagttttta cggtttttac ttttccttta tagacaaaat ataagccctg
      601 tgttggcgct ccttcaatga taaattgttg cgatttttta cagacaaaac tgttcttttt
      661 ttcgatatat tgttgcattt tttctaaatg aagatgtttt ttgataaaac aattttcatt
      721 agaacagctc tcacaaattg cagttgcttt cttcatatta tataagagtt tatgggtaca
      781 aattttgtgc ctcgctattt attttgtcaa acttataaaa tatttttcaa tttatacgta
      841 tttatacgta aaaatactta tttttacatt tttaaaagtg atgtactaag tttttcaata
      901 tataaagaat tccgaaaatg caaaattcag aaataaaaac tacgtgttcc tattgtggtg
      961 ttggatgtgg aattattgta accaacgatt cgaaaaatgg ggtagaggta aaaggagatg
     1021 taaatcatcc agtgaatcga ggaatgcttt gttccaaagg aatgaatcta cactatgtgg
     1081 ttaacgacac ctcagatcga attttatatc ccgaaatgcg ctggagcaaa tcgcatccta
     1141 aagaaagagt gagttgggat gatgctttag aacgtgctgc tgctgttttt tcttcgatta
     1201 ttaaaaaaca tggtcctgat agtgtggggt tttatgtttc gggtcaatgt ctaaccgaag
     1261 aatattattt gattaataaa ttggtaaaag gatttttaaa aaccaataat gtcgatacca
     1321 attctcgatt gtgtatgagt tcggcggtag tgggttacaa aaagactttt ggcgaagata
     1381 tagttcctat tgcttatgat gatattgaac tagcagatac gtttttgatt actggagcca
     1441 atcctgcttg gtgtcacccg attcttttta gaagattaga gcaacacaaa gaaaaaaatc
     1501 cgaagacaaa aatcattgtt attgatccta gaagaacaga ttctgctgca ctggccgatt
     1561 tgcatttgca aattattcct gggactgaca ttattctgta tcacgccatt ggaaaaagat
     1621 taatcgaaaa aggatatatc gacaacgatt ttattaaaaa tcacaccgaa aattttaatt
     1681 tatataagga cttggttctt tcaagttcgt tcgaaaaagc atctaaactt tgtggagttt
     1741 ctgtaaacga catcaaacta gctgccgata tgataggaaa agccaaagga tttatttcgc
     1801 tttgggcaat gggactcaat caaagtgcga ttggagtaga taaaaacacg gctttgttaa
     1861 acctttcttt ggtaactgga caagtaggta aaccgggttc aggccctttt tcattaacag
     1921 gtcagcccaa tgccatggga ggaagggaag ttggaggaat ggccaattta ttagcggttc
     1981 acaaagaatt aaacaatccg acgcatcgaa aagaagtagc cgatttttgg ggagttaatg
     2041 aaatttcaga aaaaccaggt ttgaccgcaa ccgaaatgtt tgatgcttta gagtctggaa
     2101 aaatgaaggc tatttggatt atttgtacca atcctttagt gagtttaccc gattctcgaa
     2161 gagtggaaaa agcattgcaa aacgctaaat ttgtggttgt tcaggacatt tcacacaatg
     2221 ccgatacctc aaaatttgcc gatttattgt tgccagcagc aggttggtta gaaaaagaag
     2281 gaacaatgac caattcagaa agacgtattt cctatcttcc aaaaggggta aatgctccag
     2341 gagaagcact ttcggatgtg gaaattttga ttcgttttgc ccagaaaatg aatttcaacg
     2401 ggttcaattt taataccacc gaggaagttt acaaggaata ttgtatgatg accaaggata
     2461 ccaaaatgga tatttccttt ttgaattata acagactcaa aaacgaaggt actttccaat
     2521 ggcccgttcc tgattatgga catccaggaa caccacgatt atttaccgat aaaaaattct
     2581 atacaccttc gcaaaaagct atttttaata tcccaacggc tatcgaaaat acatcggaac
     2641 agcctaattc acaatatcca ttcattctga ccacaggtag aattcgtgat caatggcata
     2701 cgatgaccaa aacgggaaaa gtgtcgcgat tgatgacgca tattccaagt ccgatgctag
     2761 aaataaatcc gattgacgct tttaaatcgg gaataaatgc aggtgatatt gttgtcgtta
     2821 ctagtaaaaa cggaactgta cgtgtcaaag caaaaatcac cgatagtatt cgtgaaggag
     2881 tagttttctt gcccatgcat tgggggaaac aattggaaaa cgatttgaat cgaaccaata
     2941 atttgaccaa tacgcttgtt gatccaacat cgaaagaacc ggattttaaa ttcacgactg
     3001 tttctataga aaaatatgta aaagctttcg agaaaattgc tgtagttgga gcaggagcag
     3061 ctgcatttag attcatacaa aactaccgcg aaatcaatac taccgatgaa ataattgtat
     3121 tttcgaacga agaaaacccg ttttacaatc gagttttgct tccagaatat atgacgggtg
     3181 aatttacttg ggaacaactt caaaaaatca aagaagaagc cttgagtaag ttgaatatca
     3241 cgatgaaatc gaatatctct atcgataata taaatactca agaaaaaact attttggata
     3301 gccaaggcaa tctttacact tttgattcct tgattttggc aacaggaagt cgtccgtttg
     3361 tgcctgaaaa tgcgcaattg catttaccag gacgttttac gatgcgtaga aaaagtgatg
     3421 ccgatagatt gaaaaactat ttagaaagta ccaatctacc agccgaagag caacacgttg
     3481 ttataattgg tggcggttta ttaggtttgg aattggctgc agccttaaaa cataaaaaag
     3541 taaaaatcac cattattcaa agagcatcca gattgatgga acgtcaatta gatcgaattt
     3601 cttctaaatt attggccgaa gaagtgcaac ttcgtgacat tcaaatttat tttgacaacg
     3661 aagtaagtac cgttttcgaa actgataatc cgaatgaatt ggaaattgcc ttgaaaagtg
     3721 gtcgaattat tactgccaat gctattgttt acaccattgg aacgattcca aatatcgaaa
     3781 ttgctaaaga gtcagggctt gcctgcggac gtggtgtgaa ggttaatcag tatttgcaat
     3841 cttcgaatcc tagtattttt gccattggcg aaattgctga atttaacaat caattgtttg
     3901 gaattacctc tgcagccgaa gagcaagccg atattttggc taattttatg gcaggtgata
     3961 ttagcagttt ttacaaagga tctgttttga tgaatatact aaaactagaa gatatcaatt
     4021 tgtgtagtat tggtcaggta gaagttcccg aaaatgatga ttcctatgag gaaattgttt
     4081 ttgctgattt aagacaacgc tattataaaa aatgtatcgt caaaaacgat ttgttggtgg
     4141 gcgctatttt gatgggtgat aaaaatgagt ttgccgaatt taaaaccttg atcgaaagca
     4201 aaatagagct agccgataaa cgcaatatgt tgcttagagg aagttctagt gccaaaccag
     4261 ttttagggaa attggtttgt tcatgcagcc aagtaggaag cggaaatata gaagaagcca
     4321 taaaaggtgg tgtgaccaat tttaccgaat tgtgcaaaac cactggtgct ggactaggtt
     4381 gtggtagttg taaaaccgaa gtaaaagata ttttagcaaa atgcaaataa gtttatagtt
     4441 tgttgtttat agtttgttgt tggacaacta taaaccataa accataaacc ataaactaat
     4501 aattatgaaa aacacacgat taatagtaaa aggaggcgtg atttcgccag gtgaattacg
     4561 agaaattgtc aatatgagtt tggaacaagg attgaaagcg atttcttttg gttcaagaca
     4621 agatattatt tttccagaag attttttgcc catcggaaaa gacaaaaccg ataaatattt
     4681 ttttgtttat cctgatgaaa acagtggaaa taatattgtt tcttcctatg tttcgaccga
     4741 tatttttacc aatacgcctt ggcttactgg aaataaattt ctttatattt tagaacaatt
     4801 caaggaacag cctcagttaa aagtgaatat aaccgaccca aaacagcagc tcgtgccatt
     4861 gtttacgggg catatcaatt ttgttgcttc gcatcacgag gattattggt atttgtatat
     4921 tcgcttgcct aattgggata aaatggaagt gtatcccgta cttatttaca gttggaatat
     4981 tgccgaagtc tattatgcca tcgaaaaaat acaaattgaa gaacctgata ctgttgaaat
     5041 gatttttcaa ttggtgaatg atagtttgga tactaataac agaacgattg acaaaccttt
     5101 gcagcttccg ttttatcctt ttccgtatta tgagggaatg aataaattag gaatcgatca
     5161 gtattggctg ggattatatt ggcgaaataa tttgtatgat ttagactttt tgaaggaaat
     5221 gtgtgatttg tgttttgatt gtaaaatcgg aaaaatttgt atcacacctt ggaaatcatt
     5281 cattgtgaaa ggaattccaa aagatagaaa actcgattgg gaaaaattct tgggcaaaaa
     5341 aggaattaat gttcgtcatt ctttattaga acttaattgg catttaccag ttgccactga
     5401 ttgggcttta aatctgaaaa catttttggt tcgaacctta gatcaattcg atgtaagtac
     5461 ctatggattg acctttggaa tatcagaata caaccgcgaa ggacattatt ttacttcgat
     5521 tgtagtcgaa aaaaaccaaa tgcctaaagc attggaaaca atgaaaatta gagcaaccta
     5581 taatgtttta tatgctaaaa actttgatcc taataccaag gagtatattg ttctggccca
     5641 ggatgttgat aagcaagaat taccgacaat tttaattgaa ttgagcaaga aatattttga
     5701 agaattaggg aataccgttc tagaaattaa ccaaccaatt gtcaaaaaag agttgcaaga
     5761 aagggatatt catcaatgtt cagaatgtct aacactctac aattcagaat atggcgatgt
     5821 ggctcaagga attcaaaaag gggttttgtt caaagattta ccagaagact attgttgttc
     5881 gttatgtgaa tcgcctaaaa ataattttat aaactttcta gaaaaagtat aattttatac
     5941 cttattaaaa ataattttgc cttaatactt ttacaatcaa aatactatga atactaagtt
     6001 caaaaccgta agttcttcaa atatcagcat ttctcagttg atgctgcctt ctcacactaa
     6061 ttttagtggc aaaattcacg gaggtttcat tttgtccttg ctggatcaaa ttgcctttgc
     6121 ttgtgcatca aagttttcag gaaattattg tgttaccgct tcggttgata ctgttaattt
     6181 tttgaaacca atagaagtag gagagttggt tactatgaaa gctaaagtga attatgttgg
     6241 aaaaagctct atgattgtag gtattcgagt cgaagccgaa aatattcaga ctggagagaa
     6301 aaatcattgc aattcttcct attttacaat ggttgcaaaa gatgaaaatg ggaacaaagc
     6361 attggttcca ggtttgattc tctcgaatgt agaagaggtg aggcgttttc aaaattgttt
     6421 gaaacaaatt gcattaaaaa aacaaagtga cgctcaaaaa aacagcattg attttggttc
     6481 catcgaaagt ataatgagtt caaatttata taatgtaaag ctagatttaa aatgagataa
     6541 ttagtttgta atgagttaaa cagataatgt ttaaaaataa atcccgataa gggatttttt
     6601 gtttttaatt ttaattattt tgctatccat tttctgtcct gacttaaaaa aagggacaca
     6661 gattatacag atttgagcag attaccacag attaaaattg ataagatccg tttttatccg
     6721 tataatctgc gtgccatttt attgtttagt ccgcataaaa actctaattt ggctcttttg
     6781 cgattcttaa taaaacaaaa gtgataaaaa gtgaacaagg acagcacacg agttaaaact
     6841 gtaattagac gatttattag taactttaaa aataattaac ccttaaaaat ttaactatga
     6901 aaaacaaaat cacattcgtt ttaagtcttt tatttggatt gatgttcatt aatgctgggc
     6961 tcaataagtt tctgaattat atgccaatgc cagacaattt gcctgagaaa atgcaaaaag
     7021 caatggctgc ctttatggaa attggttgga ttatgccgct tgtgggaata gtcgaaattt
     7081 tgggaggttt gctcatcatt ataccaaaaa caagagcatt gggagctttg atgattttcc
     7141 caattatggt tgggattgtg ttggtgaata ttgttcagga cactagcggt ttacctattg
     7201 ccgcagtttt tagcgctatt ttgctttgga taatgtatga aagcaaagaa aaatacgcac
     7261 ccttgtttaa ataaaaaaca atttaaaata ccaaaaatat ccctaaacac ttttagaatg
     7321 tttagggata ttttttgttt caagagaggc gatttttaat gagaactaga taatttattt
     7381 gtaaattgat gtatttggct tgctaattct cgtagaatta atttttccga tttttttttt
     7441 tttttgtaat attacaagaa aaaaaaatga aaccaacaac acttattaaa gtcttgattc
     7501 tgattttaac agcccaaaaa tcgatagccc aggattcgaa aacggcaaca actttaacag
     7561 atagtaaata cacaatcgaa aactgcgtca atcaattcga attagagaaa gccacaaaga
     7621 ctaaagtagg ctatcaatat tggtttgccg ataaaaattt cactctcgaa aatactctta
     7681 aaatgagtat cgttgagccg ggtaaatcaa cacatgcacc acaccatcat atcgaggaag
     7741 aattttttta catccttgaa ggttcggctt ccttcttttt agatggaaaa acggttgtcg
     7801 tggggcctaa tacaagttta tattgccctt caaatgtaga acacggaata agtaatgcgg
     7861 gtcaaaccga attaaaatat ttggttatta aaaaagatag gaaagagaag tagtaaaaaa
     7921 gacttggttt tataaaataa aaaaaacctt aaactcctga tattcagtgt gtttaaggaa
     7981 ttttttgtga cctcgactgg attcaaacca gtaaccttct gagccgtaat cagatgcgct
     8041 attcagttgc gccacgaggc cttattgcgg gtgcaaatat aggtaatttt tgcaatggtg
     8101 caaacctaaa tgcgaaatta tgttgatttt ttttcaaatc tgaaatataa agtctgcaat
     8161 ctaaaatcaa caatctaatg ctcttgtagt tacataatat cggtagccaa taatcgctag
     8221 ttgccatttt ttggctttag ctaaattaag tcctcttttg gtaaaactag gtagaattag
     8281 cttgtttatt tgggctagaa ttttgaacat aattattgcg ttattatttg gtattatgaa
     8341 ctaattattt gttttttgtt ttgaataaag ttcctccatt tttttgccat attctcccat
     8401 ttgctcttca taaactttca tttgctcttc ataaactttc atttgctctt cataaatttt
     8461 catcttctgc tcaaaggctt tcatgttttc atcggaattt tgtgcttcaa gttgcatctt
     8521 ttttacttga agttcatact catttatttg tttctcataa tttttccaca tctctttgtt
     8581 ttctgttgga gatccttttg gtgattttgg agctttagga aaaacaggcg gttttggagt
     8641 tttaaatgta aatgaaggtg gtacaggtgg tgttggtggt gttggaatat caatattatc
     8701 cgctgattga tgtattgttt tggttgtaat tctaattgtt ttttcatttt tattgacatc
     8761 cattttggta atggtatttt gatctaaaag caagagttcg tttttatcta ctttttcgtc
     8821 attaatataa atgtcatttt cattgagggt tttagagaat tttttggtga caattcgaat
     8881 cgttcctttg tcagcttttt tagtaacatc tatactttca atttcatttg aatctaattt
     8941 atctaaatcc tcctgactca ctttgcctcc atcgataaaa atttcttttt caattggcaa
     9001 agtctcttta gcagatgcaa tactgttttt agagttgatt acaccctcgt cagtaccaaa
     9061 atcaacagtc agttttccat tttcattttt agaaatgata attccaaatg ttttgatagc
     9121 ttctgtccct ttagttgcca ttttttttgt aatttctttg ccttttttca aatcaacttt
     9181 aatggcagtt aattcatttt tagcgtttct ttctaaaccc gaaaaagcaa gtgttacatc
     9241 gtatttttcc tttaaagttt taattttttc ctttaattct gcatcggtag tatttttgtt
     9301 tattttgtaa acatctaccg aattttctac tgtttttgaa ttttcaacta cagtttgttc
     9361 tgcttctttc tcctgtgcaa tgactttcac ttgaaagaaa aatacaaatg cacctaataa
     9421 tggaagaaca agtaaatact tccaagaatt ccttttgttt gattgatttt tgtttaacat
     9481 aacgattcgt tttttgatta atgattgata aaaatgattg gtaatagcaa cacaattttc
     9541 ctgtgttgtt atttttaaaa gcgtcaattg gtacgctttt ttgtcggaaa tgattttaga
     9601 agcttcattg tcggcgatga attccaggtt ttgaacgatg gcttttttgt acaaccacat
     9661 tagtgggttg aaccaaaaaa caacacaaaa tagtcttgaa atcaatacat cagcggtgtg
     9721 attttgggtg ctgtgcactt tttcgtgttc caaaatgttt tccaattccg tttcgctgta
     9781 cagtgatgag ttatacacga tggaattgaa ataggaaaaa ggagcaatgt tttcggtaac
     9841 gtcaataaat tggaaatcag cttgatgctg gatggttttt cctttcaaaa ctttggataa
     9901 actgtaaaaa tctaaggcaa atttcaccaa gaaacccaca attccaatcg aataaacaac
     9961 ggcaaaaacc agataccaat tgatttcaaa agcttcattt tcgacagtcg tggtcgtcat
    10021 cggaatattt gaccaatcga aattagttgg agtaggttcg acccaaacta ttttcgtgaa
    10081 aacaaccaat ggcaatacaa ccgaagtgat taatcccgct aataaaaacc aacgattgct
    10141 gttgaaaaaa gtttcttttc gcaacaaaag aatgtaagcc aaataaaata ttccaataag
    10201 accagaggct ttgaccaaga aaatgaatag cgtttccata gtattacttt ttttcgatca
    10261 tggctaaaat ctctcgtaat tcttcggctg atattttttc ttccttggca aaaaacgaca
    10321 ccatattttt ataggaacta ttaaaataag tatcgatggc cgtgttcata aaacgctttc
    10381 tgtagtcttc cattttcacg atagggaagt attgatgtgt gttgccaaaa gcattgtgcg
    10441 acacaaaacc tttttcttcc aggttacgga taatggtcga aagcgtgttg taatggggtt
    10501 gttcttcggt aatttctgct agaacttctt ttacaaaagc tttttcgagc ttccataaaa
    10561 tttgcataat ttcttcttct ttgttggtga gtttttgcat agttgtataa tttgtgtcat
    10621 cctgagcgta acgaaggaca aacgtacaac tatattttta gttaataaac tatttttata
    10681 gttatttaac taaattttaa gtttaaatac aataaagtct aataaattag ctgaaaaaca
    10741 agtgtttata ttttgtttgc tagtcgttcg gtctgtaaca ttcgatgcag ccaaacgcaa
    10801 cagagtaaag caagtatagc taaacttccc attacaatcc aattggtttg atagccatag
    10861 ttgtcaatga tatccaatcc tgtcttggag cagacaatat gtgctagact aaaactcatt
    10921 gtaaataagc ccatatattg tccttcttgt ccttttggag ctcggtttag agccacagca
    10981 ttcgaaaaag gaaaagcaaa tatttctcca agtgagagca aaatgatact gattatcaaa
    11041 ataaaaatcc aagaatctaa taataaaaca aaatatccta aggcaacaca taatgcacct
    11101 aaggagatga ttttgatttt gttggtttgt tttctttcaa attggctcac aatgggcatt
    11161 tccaagaaaa acaccaaaag tccgttcaag gaaagtagca ttccagtttg aacttcggtt
    11221 aacccaaatt tttcgtgatg atacagcgga agggtagtaa atatttgaaa aaacaccatt
    11281 acaata
//
LOCUS       DAOSNN010000057        11051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5892_length_11051_cov_4.971808, whole genome shotgun sequence.
ACCESSION   DAOSNN010000057 DAOSNN010000000
VERSION     DAOSNN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11051
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5892_length_11051_cov_4.971808"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLQ70_04495"
     CDS             complement(<1..832)
                     /locus_tag="PLQ70_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQE33908.1"
                     /translation="MVISNKPLQFIIAILIACLPIVGLANNPTEHTPAHTETTAVAHE
                     ASGEPKDEKSEIKEKIDEKKAHHVLDAHEFSLFEDEETGVHYGFPLPIILWDNGVQIF
                     SSSKFNHGESVAESNGNYYKLGHNEKIYKTDAAGTMTGDEHHPTNPQVLDFSITKSVF
                     SMMVVALLMFFLFSGLAKSYAKNGSIAAGAGRFFEPIVLYVRDEIAIPNIGEKHYKRY
                     MSFLLTVFFFIWFLNIFGLTPLGINVTGNIAITAGLAIITFLITTFTANKNYWGHIFW
                     M"
     gene            complement(1355..1588)
                     /locus_tag="PLQ70_04500"
     CDS             complement(1355..1588)
                     /locus_tag="PLQ70_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQE33909.1"
                     /translation="MTDNPNPKKQTNNKWLALINIPIQMGAIIFLFAYFGNWLDEKYP
                     NSHTIFVKILTLVGVAIAFYNINRQLKDINKSS"
     gene            complement(1551..1961)
                     /locus_tag="PLQ70_04505"
     CDS             complement(1551..1961)
                     /locus_tag="PLQ70_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQE33910.1"
                     /translation="MFEKKSKSYTEFLGKTNRIVEGTILKGDIISNADFRLDGELTGN
                     FQTKGKIVIGPAGSVTGDIICKNADIEGKFKGKIQVVEILNVKNKASIYGEVICGKLS
                     VEPGADFSASCIMKTNSKNTIANDGQPQPEEANQ"
     gene            complement(1965..4793)
                     /locus_tag="PLQ70_04510"
     CDS             complement(1965..4793)
                     /locus_tag="PLQ70_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE33911.1"
                     /translation="MINCFPLKTKIFKPIFFLLFLIFLVACSTKKDTFLARNSHALST
                     RDNILYNGQIALNKGVEGIKSSNQDNFWKRLPIERMQLKDEGAPQGSSKNADFEAAEA
                     KATKAIQKHSMVIDGQEKNYQIDEAYLLLGKARYYDQRFFPALEAFNFILYKYPTSSN
                     INQAKIWREKTNMRLGNDAQVIKNISKLLKEKKLKSQVVADANALLSESFLNLEEKDS
                     AIAKLKIAEKNTKINAEKARYRFILGQLYQELGVRDTALIYYQAVIDMNRKVDREYLI
                     QAYAKRAQLFDFENGDKEAFVKTYNKLVADRENRPFLGTIYYEMGVFYDKQNDQDLAK
                     EFYNASLKTKSPDNYLLASNYRNIGDMHFKNTDYYTAAKYYDSTLVKLDVKTREYIHI
                     KKVRKDLDEVIEFESIAKTNDSILNVVAMTDANRISYYEEYIGKLKKADEAKKLLEEK
                     EKERQKNIDRNNISSEDDPATGVLQGGKAPQKKSLVPPTMAGVSSNDASNVFYFYNPS
                     TVAFGKIEFKKKFGNRALSGNWRMPTTKTDATTDDSNNSGTTTPETQVAETEMPQYTT
                     AFYIGMLPNSQKEIDSIAKERNFAYYQLGVIYKEKFKEYKLASNKLEQLLKQNPEEKL
                     VLPTMYNLYKIYQITDAAKAEDMKNRISSQYSDSRYAQIINNKNPNTVTESETAEAEY
                     GKWYQLYQEEQFVVILEKIDGIIIQYYGSEIVPKFELLKANTIGKLRGLDAYKKAMQF
                     VADNYSTSEEGKNAKEILTTQIPLLEKMNFTKTESKNWKVLYKVGKIEDKATKALEDK
                     IKKFIASENLTRLNYTFDIYTEKENFITLQGIKSEAYAHNIADIMKVDKKYKITEPAI
                     VISSDNYQVVQIKKNLDEYLSPPKTTVVPTQSVAPTPAAQQPPVNNSKTPDQAAPPAS
                     KSARTRPPGGVPNSSGNDKPNATSSPKQ"
     gene            complement(4862..5557)
                     /locus_tag="PLQ70_04515"
     CDS             complement(4862..5557)
                     /locus_tag="PLQ70_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE33912.1"
                     /translation="MINVQNLSKTYNGTTVLKIDSLTIPKGQSFGLVGNNGAGKTTFF
                     SLILDLIQPSTGHIINNDVQVNTSENWKPFTASFLDESFLIGYLTPEEYFYFIGDLRG
                     QNKADVDALLTKHEEFFNGEILKNKKYLRDLSKGNQKKVGIIATLIGNPEVVILDEPF
                     ANLDPTTVIRLKKIIKELAENPDVTVLVSSHDLLHTVEVCDRIVALNKGEVVKDIQTS
                     AETLQELETFFAV"
     gene            complement(5631..7100)
                     /locus_tag="PLQ70_04520"
     CDS             complement(5631..7100)
                     /locus_tag="PLQ70_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5687 family protein"
                     /protein_id="HQE33913.1"
                     /translation="MIRKFLYLEWKAFTRSASFGTNLAVKILVGFLAVYFTLVFLAIG
                     IGAFYILKELKLDPIVTINKFMIYYLLVDLIIRLLLQKIPVMNIRPLLVLPIKKPIIV
                     HFSLGKTVLSFFNFVHAFFFLPFSIVLIYNGYDVLSVILWHTAMVSLIYINNFVNILL
                     GNKDNLLAVFIGIVATLGGLQYYGYFGITSYTAPFFEALFNTYWAFAIPEIGLIGLYY
                     VTFHYFKSNLYLDAGLSSKHDIAKTEDLTWLNQFGTIGTFLKNDIKLIKRNKRSKTTV
                     GMSVMFLFYGLLFFTNGIEAYNNPTMHIFAGIFVSGGFLITFGQFVPSWDSAYYQLMM
                     TQNIPYKGYLSSKWWLMVIATIVTTIIASFYLYFGWQVYMTIVVGAIYNIGVNSHLVL
                     LGGAFTKTPIDLDSGKGAFGDKKAFNIKTMLISIPQLALPVLVYWIGAKLANANVGLA
                     LIALLGIIGFAFKNKAFSMIEKIYKTEKYATIASYKQKS"
     gene            7224..8276
                     /locus_tag="PLQ70_04525"
     CDS             7224..8276
                     /locus_tag="PLQ70_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HQE33914.1"
                     /translation="MPLFKKLIIKEVKRETKDAVSILFNVPEEFKSHYTFVAGQYVNL
                     RLTLDGQEIRRAYSICSSPESGELRIAVKAVKNGAFSQFANTKLKAGDVLEVAKPEGK
                     FTFEPEIHNLKNYAAFVAGSGITPAISILKSVLKSEPQSSFVLVYGNKSPEETIFHQE
                     LHDLQLKYTGRLFVHFVYSQAKADGALFGRIDKSVVNFVLNNKHKELEFDKFYLCGPE
                     EMINTVSKVLKEHNISDSAIKFELFSSSSVENLEASSHEGHTKITMTVDDDETTFEMS
                     QKQTILEAALKQGIDAPYSCQGGICSSCLARVKSGTAEMKKNSILTDSEIAEGLILTC
                     QAHPTSAEIVVDFDDV"
     gene            8465..8782
                     /locus_tag="PLQ70_04530"
     CDS             8465..8782
                     /locus_tag="PLQ70_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQE33915.1"
                     /translation="MKKQTLTSFGEQIRTLRETAMLSLRQVAGDLNIDPSLLAKFERN
                     ERQPTKEIIKLISNYYNIDNNSLLMQCLSDQIAYKMLDEDDGLEILKVAEKKVIYLKS
                     KKQ"
     gene            8779..9756
                     /locus_tag="PLQ70_04535"
     CDS             8779..9756
                     /locus_tag="PLQ70_04535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HQE33916.1"
                     /translation="MSKTLSRNKELPTFFGELQLKGLRLDNNKNSETPSLFYQHPNGQ
                     IWNGNSIEWMKSLDDECVDLVFADPPYNIKKADWDTFESQEAYINFSMRWIEEASRIL
                     KPHGSLYICGFSEILADIKHPATKFFKSCRWIVWNYKNKANLGKDWGRSHESILHFRK
                     TKNFTFNINEIRIPYGEHTLKYPSHPQAETSQYGNGKSSSQNWEPNPLGAKPKDVLEI
                     PQDIIEVPTTCNGMHEKTPHPTQKPEELLRKIILASSNPGDTILDPFCGSGTTPVCAE
                     QLNRKWLSCDLSAEYLDWACNRIEMVEEWTIEQWIKYDFDNQKRRNSIR"
     gene            9753..10649
                     /locus_tag="PLQ70_04540"
     CDS             9753..10649
                     /locus_tag="PLQ70_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33917.1"
                     /translation="MSIKKILTKALDKNNFKNLQGYIDFSQQYLDFVAEHLQAVIISQ
                     NENHYNFYQYDKQGNYQITRPINSNLMYKTDTFSVASTKFVEMLQNLKEVKPTLEIER
                     QILNNMTYTMQQSIGGALDAIEGGKSNFARKINGDLFENLIRLIINETGIEAKSGIIK
                     VPVIIDNVEEFKMNYQHDLIIENPDEIKLIGSIKTSSKDRIDKIFIDKFLYNKLTENP
                     TPHIAIFLHDVQRKNTRKENEYGISGTFLPGHFKGYTVKLNPLDGVYYCDIRPNMRTE
                     KILKDHIKTFDNLLIDDIWKFL"
BASE COUNT         3618 a         1980 c         1842 g         3611 t
ORIGIN      
        1 gcatccagaa aatgtgtccc caataatttt tatttgctgt aaacgttgta ataagaaatg
       61 taataatggc taaacctgct gtaattgcaa tatttcctgt aacattgatt cctagtggcg
      121 ttaatccaaa aatgtttagg aaccaaataa agaaaaagac agtcaacaag aaactcatgt
      181 agcgtttgta atgtttctct ccaatgtttg gaatggcgat ctcgtcacga acatacaaaa
      241 caattggctc aaagaaacga cctgctccag cagctatact tccgttttta gcatatgatt
      301 ttgccaatcc tgaaaacaag aaaaacatta aaagtgcaac aaccatcatt gaaaaaacac
      361 tttttgttat agaaaaatct aaaacctgtg gatttgttgg atgatgctcg tctccggtca
      421 ttgttcccgc agcatcggtt ttataaattt tttcgttatg acctaattta taatagttcc
      481 cgttagattc agcaaccgat tctccgtgat tgaattttga tgatgaaaag atttgaactc
      541 cattatccca caatataata ggtagtggaa aaccataatg aactccagtt tcttcgtctt
      601 caaataaaga aaattcatga gcatcaagaa cgtggtgtgc ttttttctcg tctatttttt
      661 cttttatttc tgatttttca tcttttggtt cacccgaagc ttcgtgagca actgctgttg
      721 tttcagtatg tgctggcgta tgttccgttg gattattggc taaaccaact attggaagac
      781 aggctattaa aatcgctatt ataaattgaa gtggtttgtt tgaaatcacc ataactgtta
      841 attatcttaa tttgtacttt ctgaaattgg gtgcaaaggt acattttaaa ttaatatgtt
      901 aaaaacatta aataaaaaaa ataacaattt tatcttttaa aagtcaattc attgcttatt
      961 atttaacatt ctgacagtta caatggtttc tatagtcaaa aacagagcga aaatgataaa
     1021 aaagtcaatt ttttcggttc ttaaatttgg atttcccgaa ttcaggatgg gtaataaaac
     1081 aacatatgaa ataccgattt tcaagcaagt tagcaaaaga aatgtaaatc ctacgttatc
     1141 aatacttttt tcataaacct taataagtat gaagataatt aatagtgaag aaataaagaa
     1201 aaacccataa acaacctcta ttggaaagtg aaatccctga agtttaggat ggttttcgtt
     1261 taagaaaaaa accaatttgt gtattgaata aaccagaatt gaaaggaaaa ctacttcaat
     1321 taggggtcgg tattttttta aattcatcac tattttatga agatttatta atgtctttca
     1381 gttgcctatt gatgttgtaa aaagctatgg caaccccaac tagtgttagg attttgacaa
     1441 atatagtatg tgaattgggg tatttctcgt ccaaccaatt tccaaaataa gcaaacaaaa
     1501 aaatgattgc tcccatttga ataggaatgt taatgagtgc caaccattta ttattggttt
     1561 gcttcttcgg gttggggttg tccgtcattt gcaatcgtgt ttttggaatt ggttttcatg
     1621 atacaagaag cactaaaatc ggcgcctggt tctactgata attttccgca aattacttcg
     1681 ccgtaaatac tagctttatt tttcacgttc aatatttcta ccacttgaat tttgccttta
     1741 aatttgccct caatatcagc gtttttgcaa ataatatcac cggttacact tcctgctggg
     1801 cctatcacaa tttttccttt ggtttgaaaa ttccctgtaa gttctccatc taatctaaaa
     1861 tcggcattag aaataatatc tccttttaga attgttcctt caacaattcg attcgttttc
     1921 cccagaaatt cggtgtatga ttttgatttt ttttcaaaca taatttactg tttaggcgat
     1981 gatgttgcat tgggtttgtc attgccacta ctatttggca cgcctcctgg aggtcttgtt
     2041 ctagcagatt tgcttgcagg tggcgctgct tgatcaggtg ttttagaatt atttactggt
     2101 ggttgttgag ctgctggtgt tggagcaact gactgagtag gaacaacagt cgtttttggt
     2161 ggagacaaat attcgtctaa atttttcttg atttgaacta cttgataatt gtcgcttgaa
     2221 attactatag ctggttctgt aattttatat tttttgtcaa ccttcataat atcggcaata
     2281 ttatgagcat atgcttctga ttttattcct tgaagggtta tgaaattttc tttttcggta
     2341 taaatatcaa acgtatagtt aagtcttgtc aaattttcgc ttgctatgaa ttttttaatt
     2401 ttgtcttcaa gtgcttttgt tgctttatcc tctattttgc ccactttgta aagcactttc
     2461 cagtttttag attcggtttt agtaaaattc attttttcta acaacggaat ttgagtcgtt
     2521 agaatttctt tagcgttctt gccttcttcg ctagttgaat aattatcagc tacgaactgc
     2581 attgcttttt tataagcatc taaacctcta agtttcccga tggtgtttgc ttttaataat
     2641 tcaaattttg gtacaatttc actgccataa tattgtataa taataccatc tattttttcg
     2701 agtattacta caaattgttc ttcctgatat aattgatacc attttccata ttcagcttct
     2761 gcagtttctg attcggttac tgtattagga tttttgttat taataatttg tgcatatcga
     2821 gaatctgaat attggctaga aattctattt ttcatatcct cagccttggc agcatcggta
     2881 atttgatata ttttatataa attatacatt gtaggcaata ctaacttttc ttcgggattt
     2941 tgctttaaca attgctccaa tttattgcta gccaatttat attccttgaa tttttctttg
     3001 taaatcacac ccagttgata gtatgcaaag tttctttctt tagcaatgct gtcaatttct
     3061 ttttgcgaat taggaagcat tccgatataa aatgcggttg tatattgagg catttcggtt
     3121 tcggcaactt gggtttctgg agttgttgtt ccgctgttgt tactatcgtc tgtagtggca
     3181 tcagttttag tagttggcat tctccaattt cctgatagag ctctatttcc aaattttttc
     3241 ttgaattcta tctttccaaa agctaccgtt gacggattat aaaaatagaa aacattggaa
     3301 gcatcattcg atgaaacacc agccattgtt ggtggtacaa gtgatttttt ttgaggcgct
     3361 tttccgcctt gtagaactcc tgtagcagga tcatcttcag aagaaatatt atttctatca
     3421 atattttttt gcctttcttt ttccttttct tctaaaagtt ttttagcttc atctgctttt
     3481 tttaatttac caatatactc ttcatagtat gaaattctat tggcatcggt catcgcaacc
     3541 acattcaaaa tgctgtcgtt tgtttttgca atcgattcaa attcgataac ttcatctaaa
     3601 tcttttcgca cttttttgat gtgaatgtat tctcttgttt taacatccaa ttttactaaa
     3661 gtactgtcat aatacttagc tgctgtgtaa taatcggtgt ttttaaagtg catatccccg
     3721 atattcctat aattggaagc caataaataa ttgtctggcg actttgtttt taaggatgca
     3781 ttataaaatt ctttggccaa atcctgatcg ttttgtttgt cataaaaaac gcccatttca
     3841 taataaatag ttcctaaaaa gggacgattc tctctgtcgg caacgagttt gttgtaggtt
     3901 tttacaaaag cttctttgtc accattttca aaatcgaata actgcgctct tttggcataa
     3961 gcctgaataa gatattctct atctactttt ctattcatat cgataacagc ttgataataa
     4021 atcaaggctg tgtcgcgaac tcccaattct tgatacaatt gtccaagaat aaagcgatag
     4081 cgtgcttttt cggcgttaat tttggtgttt ttttcggcaa tttttagttt agcgatggca
     4141 ctgtctttct cttctaaatt caaaaacgat tcagacaaaa gcgcattagc atcggctaca
     4201 acttgtgatt ttagcttttt ctcttttaaa agttttgaga tattttttat aacctgagcg
     4261 tcgtttccta aacgcatatt ggttttttca cgccagattt tggcctgatt gatattacta
     4321 cttgtaggat atttataaag tataaaattg aaggcttcca atgcaggaaa aaaacgttgg
     4381 tcataataac gtgcttttcc taaaagcaaa taagcttcat ctatttggta gtttttttct
     4441 tgaccatcaa tgaccatcga atgtttttga atggcttttg tagctttggc ttctgcagct
     4501 tcgaaatcgg cgtttttgga acttccttgt ggagcacctt catcctttaa ttgcattctt
     4561 tcgatgggca atcgcttcca aaaattatct tgattgctac tttttatacc ttcaactcct
     4621 ttattcaaag ctatttgacc gttatacaaa atgttgtctc tggtacttaa agcgtgggag
     4681 tttctggcta aaaaagtgtc tttcttggta gaacaagcta ccagaaaaat tagaaataat
     4741 agaaagaaaa tgggtttaaa tatcttggtt ttcaatggga aacagtttat catttaactt
     4801 ttaaaagcgc attttagtat aatgactggt aaaaatacgt ttctttttga aatatggaaa
     4861 tttaaaccgc aaaaaacgtt tccaattctt gcaaggtttc tgccgatgtt tgaatgtctt
     4921 ttactacttc gcctttgttt agtgcaacaa ttctgtcgca aacctcaacc gtgtgaagca
     4981 aatcgtgact cgaaaccaat acggtaacat ctggattttc agccaattct ttgattattt
     5041 ttttcaatcg gataaccgtt gtaggatcta aattggcgaa aggttcatcg agaatcacaa
     5101 cttcgggatt gccaattagt gtagcaataa ttcccacttt cttctgattt cctttagata
     5161 aatctcttaa atattttttg tttttcaaga tttcaccatt aaaaaattct tcgtgtttag
     5221 ttaataaagc atccacatcg gctttgtttt gacctcgtaa atcgccaata aaatagaaat
     5281 attcttcggg cgtgagatag ccaatcaaga agctttcgtc aagaaaagaa gcggtgaagg
     5341 gtttccagtt ttcactagta tttacttgaa catcattgtt tataatatgt cccgttgacg
     5401 gttggattaa atccaatatt agactgaaaa aagtcgtttt tccggctccg ttattgccca
     5461 caagaccaaa actttgtcct tttggaattg ttaaactgtc tatttttaat acggttgttc
     5521 cgttgtaggt ttttgaaagg ttttgtacgt ttatcatttt ttgttttgtt tttttgtccg
     5581 taaaaacatt gcaatgtgta gagacgttgc catgcaacgt ctgtacggaa ttacgatttt
     5641 tgtttgtatg aagctatggt ggcgtatttt tctgttttgt atattttttc aatcatcgag
     5701 aaagctttgt ttttaaaggc aaagccaata attcctaaca aagctatcaa tgctaatcca
     5761 acattggcgt tcgctaattt agcacctatc caatacacta aaacgggcaa agccaattgc
     5821 ggaatcgata tcaacatcgt ttttatattg aacgcttttt tatcgccaaa agctcctttt
     5881 ccagaatcta aatctatggg cgttttggtg aatgcgccac caagcaaaac caaatgagaa
     5941 ttaactccaa tgttgtaaat cgctcccaca acaatggtca tataaacctg ccatccgaaa
     6001 taaagataga acgaggcaat aatggttgtt acaatcgtag caatcaccat caaccaccat
     6061 ttagaactca aatagccttt gtaaggaatg ttttgggtca tcattaattg gtaataagcg
     6121 ctgtcccaac tcggtacaaa ttgtccgaaa gtgattaaaa atcctccaga aacaaatatt
     6181 ccggcaaaaa tatgcatcgt cggattgttg taggcttcaa ttccgttcgt aaaaaaaagc
     6241 aaaccataaa aaaggaacat cacactcatt cctacagtag ttttggatcg tttgtttctt
     6301 ttaatcaatt tgatgtcgtt tttcaggaat gttccaatgg tgccaaattg attcaaccaa
     6361 gtcaaatctt ctgttttggc aatgtcgtgt ttgctcgaaa gtcccgcatc caaatacaaa
     6421 ttacttttga aataatggaa tgtaacataa tacaacccta tcaaaccaat ctcaggaata
     6481 gcaaaagccc aataggtgtt gaaaagagcc tcaaaaaaag gagcggtata actcgtaatg
     6541 ccaaaatagc catagtattg caaaccaccc aaagtagcga caattccaat aaaaacagcc
     6601 aacaaattgt ccttattgcc caatagaata ttgacaaaat tgttgatgta aatcaaggaa
     6661 accattgccg tatgccaaag aatcacggac aaaacatcat atccgttgta tatcaaaaca
     6721 atgctaaaag gaagaaagaa aaaagcgtga acaaaattga aaaaagacaa aaccgttttc
     6781 cccaatgaaa aatgaacgat gatcggtttt ttgatgggta aaaccagaag tggacgaatg
     6841 ttcatcaccg gaattttctg caacaacaat cgaatgatta aatcaaccaa caaataataa
     6901 atcataaatt tattgatggt gactatcggg tcgagtttta attccttgag aatgtaaaac
     6961 gcaccaatac ctattgctaa aaagactaaa gtgaaataga ccgctaaaaa acccaccagt
     7021 atttttacag ccaaattggt tccaaaggaa gccgacctcg taaaagcctt ccattcgaga
     7081 taaaggaatt ttcgaatcat aattgtatcg tttgattctg tatgagtaga taaatgtaaa
     7141 taattgttac aaaaaatcta aacttataaa ccgagaactt ctatattttt atgggtttac
     7201 tatctttgca aaaaaatatt ccaatgcctt tatttaaaaa actcattata aaagaagtaa
     7261 aacgcgaaac caaagacgcc gtttccatac tttttaatgt gcccgaagaa ttcaaatcgc
     7321 attatacttt tgttgcgggt caatatgtta acttaagatt gacgctggac ggacaagaaa
     7381 ttcgtcgtgc ttattctatt tgttcatcgc ctgaaagtgg tgaattgaga attgccgtga
     7441 aagcagtaaa aaatggtgct ttctcccaat ttgccaacac caaactaaaa gcgggcgacg
     7501 ttcttgaagt cgcaaaacca gaaggaaaat tcactttcga acccgaaatt cacaacctaa
     7561 aaaactacgc agcttttgta gccggaagcg gaatcacgcc agccatttct atcttgaaat
     7621 cggttttgaa aagcgaacca caaagttctt tcgttttggt ttacggaaat aaatcgccgg
     7681 aagaaaccat ttttcatcag gaattacacg atttacagct gaaatatacc ggacgattat
     7741 ttgtgcattt cgtttacagt caagccaaag ccgatggagc gctttttggt cgaatcgata
     7801 aatcggtcgt gaattttgtc ttgaacaaca aacacaagga attggaattc gacaagttct
     7861 atttgtgcgg accagaagaa atgatcaaca ccgtttccaa agttttgaag gagcacaaca
     7921 ttagtgattc tgctattaaa tttgaattat tctccagttc ttcggtcgaa aatctggaag
     7981 ccagttctca cgaaggtcac accaaaatta cgatgactgt tgacgacgac gaaacgactt
     8041 tcgaaatgtc gcaaaaacaa accatcctcg aagcagccct aaaacaagga atcgatgctc
     8101 cctattcttg ccaaggcgga atttgcagca gctgtcttgc ccgagtaaaa tcaggaacgg
     8161 ccgaaatgaa gaaaaactcc atcctgaccg atagcgaaat cgcagaaggt ttgattctta
     8221 cttgccaagc gcatcccacc tcagcagaaa ttgtggtgga ttttgatgat gtttaaaaaa
     8281 taatttaaat gataaacgaa aacttctctg aaaagggaag ttttcgttaa cttacagcag
     8341 tcaataaaaa tatcaactaa ctttttaaac tacaaatttc tcaacatttt gtgaaaaatt
     8401 tttgtggaca actcttgtcc ataaaaaata tctttactac atttggacaa aattagacca
     8461 caaaatgaaa aagcaaacgc ttacttcttt tggtgaacaa ataaggacac tgcgggaaac
     8521 tgccatgctt tcgcttagac aagttgcagg cgatttaaat attgaccctt ccttacttgc
     8581 taaatttgaa cgtaacgagc gacaaccaac gaaggaaatt ataaaactca tttctaacta
     8641 ttataatatt gacaataatt cattgttaat gcaatgcctt agcgaccaaa ttgcttataa
     8701 aatgcttgac gaagatgacg gactggaaat attaaaagta gctgaaaaaa aagttattta
     8761 tctaaaatct aaaaaacaat gagcaagaca ttatcaagaa ataaagaact gcccacattt
     8821 tttggcgaat tacaactcaa aggcttaagg cttgacaata acaaaaacag cgaaacgcct
     8881 tctttattct accaacaccc aaacggacaa atttggaatg gtaacagcat tgaatggatg
     8941 aaatctcttg acgacgaatg tgttgattta gtgtttgcag atcctcccta caacatcaaa
     9001 aaagctgatt gggatacttt tgaaagccaa gaagcttata tcaatttttc aatgcgttgg
     9061 attgaagaag catcaagaat tttaaaacct catggttctc tttacatttg tggcttttct
     9121 gaaatccttg cagatattaa acatcctgca acaaagtttt ttaaatcctg ccgttggatt
     9181 gtttggaatt ataaaaacaa agctaactta ggaaaagatt ggggacgtag ccacgaaagt
     9241 attttgcatt tccgcaaaac aaaaaatttc acattcaata ttaatgaaat tcgtatcccc
     9301 tatggtgaac acacactgaa atatccaagc catccacagg cagaaacaag tcaatatgga
     9361 aatgggaaga gcagttctca aaattgggaa ccaaatccat taggagcgaa accaaaggat
     9421 gttttggaaa ttccgcaaga catcattgaa gtgccaacaa cttgtaatgg aatgcacgaa
     9481 aaaactccac acccaacgca aaaacccgaa gagcttttgc gaaaaataat attagcttct
     9541 tccaatccag gcgatacaat attagaccct ttttgtggct cgggaactac accggtatgc
     9601 gccgaacaac ttaaccgtaa atggttatcc tgcgacctat cggctgaata cttagattgg
     9661 gcttgcaacc gaattgaaat ggttgaagaa tggacaatag aacaatggat taaatacgat
     9721 tttgataacc aaaaaagacg caactctata cgatgagcat aaaaaaaatt ttaacgaaag
     9781 cccttgataa aaacaacttc aagaacctac aaggctatat tgatttttca caacaatacc
     9841 ttgatttcgt tgccgaacat ttacaagccg tgataatttc acaaaatgaa aatcactata
     9901 atttctacca atatgacaaa caaggaaatt atcaaataac acgaccaatt aactcaaact
     9961 taatgtacaa aacagataca ttttctgttg caagtacaaa gtttgttgaa atgttgcaaa
    10021 acctaaaaga agtaaaacca acacttgaaa tagaacgcca aatactcaac aatatgactt
    10081 acacaatgca acaatcgatt ggtggtgcat tggatgcaat tgaaggggga aaatcaaact
    10141 ttgcaaggaa aattaatggc gatttatttg aaaatctaat taggcttatc atcaacgaaa
    10201 caggaataga agccaaatcg ggtataataa aagtacccgt tatcattgac aatgtggaag
    10261 aatttaaaat gaattatcaa cacgacctta taattgaaaa cccagacgaa ataaaattaa
    10321 ttggctcaat aaaaacttcc agcaaagacc gcattgacaa aatatttatt gacaagtttc
    10381 tgtacaacaa actaacagaa aacccaacgc cacacattgc tattttccta cacgacgtac
    10441 aacgaaaaaa cacaagaaag gaaaatgaat atgggattag tggaacattt ttgccaggac
    10501 actttaaagg ttacaccgta aaactaaatc ctcttgatgg tgtttattat tgtgacatca
    10561 ggcccaatat gcgaactgaa aaaatattga aagaccatat caaaactttt gacaatctgc
    10621 ttattgacga tatttggaaa ttcttgtaac ggaataacct aagcgaataa agggaatgct
    10681 tcatacaaaa aggttttgga gttatagaaa gttatcgtat cgctaacgta tattatcgta
    10741 tcatatcgta tattttcaaa tcaaaattgc tacactaaag agtttcaaca atacaaaact
    10801 gaaaactgcg actgaaaact attttcacta tgaaatccat ccgagaaata ttcaaaacca
    10861 acccttccct tttggaagaa ccagaagtag caaaactttt ggactattga aatgaattgc
    10921 aagacgagaa gatttattta taggctattg attcgttcct cgccataact acaaaaacgc
    10981 attccataaa aaaatgtttt tttgtcttaa gttctcctct ttatcatctc catagttgaa
    11041 ccattctttt g
//
LOCUS       DAOSNN010000058        10984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5943_length_10984_cov_4.145393, whole genome shotgun sequence.
ACCESSION   DAOSNN010000058 DAOSNN010000000
VERSION     DAOSNN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10984
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5943_length_10984_cov_4.145393"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..777)
                     /gene="pdxH"
                     /locus_tag="PLQ70_04545"
     CDS             complement(133..777)
                     /gene="pdxH"
                     /locus_tag="PLQ70_04545"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HQE33918.1"
                     /translation="MEDLSNYRKSYEKSELLETNIPEDPINLFNRWFHEVEDFGGVDE
                     VNAMTVSTFGLDGFPKSRVVLLKQFTYEGFIFYTNYNSEKGRAIANNPNVCLSFFWSS
                     LERQVIIKGIAEKISENLSDGYFESRPTGSKLGAIVSNQSEIIASRSVLEESLKQLEH
                     DFEGKEIPRPNYWGGYLIRPVAVEFWQGRPNRLHDRIRYELQDDYFWKIDRLAP"
     gene            complement(936..1841)
                     /locus_tag="PLQ70_04550"
     CDS             complement(936..1841)
                     /locus_tag="PLQ70_04550"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HQE33919.1"
                     /translation="MNLTILGCYAATPRTFTNPTSQVLEIRNRLFLIDCGEGTQVQLR
                     KNKIKFSKINHVFISHLHGDHFFGLIGLISTFALLGRTTDLHIYGPKGIKEIINLQLR
                     LSNSWTKYDLFFHELESKESEVVFEDDKVIVKTIPLKHRVYTNGFLFQEKRRERKLNL
                     DAVQNYEIEKCYYQNIKNGKDITLDDGRVIENHKLTFDPVLPLSYAFCSDTVYNEAII
                     PIINGVDILYHESTFLDSEEELAGKTMHSTAKEAATIALKANVKQLILGHYSTRYESI
                     ELFKEQAETIFSNVLLAEDGKCFEF"
     gene            complement(1931..2263)
                     /locus_tag="PLQ70_04555"
     CDS             complement(1931..2263)
                     /locus_tag="PLQ70_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HQE33920.1"
                     /translation="MKVEQKGHTVTIKDTQGDLDSFRLKLTNEYKTFQKSNIIVDIRM
                     HKTLSIKDIESFLPLSKTHKKNKKSFVVVSSEFDFNAISHKLAVVRSVQEAHDIIEME
                     EIERDLGF"
     gene            complement(2336..3262)
                     /locus_tag="PLQ70_04560"
     CDS             complement(2336..3262)
                     /locus_tag="PLQ70_04560"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQE33921.1"
                     /translation="MSELSVNHLLGIKYINEKDINLIFETADHFKEVINRPIKKVPSL
                     RDITIANIFFENSTRTKLSFELAQKRLSADVISFSAAQSSVKKGETLIDTVNNILSMK
                     VDMVVMRHSNPGAAHFLSQNVKASIVNAGDGAHEHPTQALLDSYSIREKLGDVAGKKV
                     VIVGDILHSRVALSNIYALQMQGAEVRVCGPNTLIPRHIESLGVTVEPNLRKALEWCD
                     VANMLRVQNERMDVNFFPSTREYAQQYGLDKTLLDSLDKEILIMHPGPINRGVEITSD
                     VADSKQSIILNQVENGVAIRMAVIYLLASKIQ"
     gene            complement(3269..3808)
                     /gene="pyrR"
                     /locus_tag="PLQ70_04565"
     CDS             complement(3269..3808)
                     /gene="pyrR"
                     /locus_tag="PLQ70_04565"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQE33922.1"
                     /translation="MSQKVLLTSKEVNIILHRLACQLVEKHLDFSDTILVGIQPRGIF
                     LARRLKDMLEKEYNISKIALGYLDITFFRDDFRRTNKPLEANKTQIDFIVENKKVVFI
                     DDVLYTGRSIRSALTAIQSFGRPSEIELLTLIDRRFSRHLPIQPDYRGRQVDAINDER
                     VLVCWKEQDGEDAVYLINK"
     gene            complement(3949..5565)
                     /locus_tag="PLQ70_04570"
     CDS             complement(3949..5565)
                     /locus_tag="PLQ70_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQE33923.1"
                     /translation="MLSINNLSVQFGKRILFDEVNTVFTHGNVYGVIGANGAGKSTFL
                     KIISGEMDPTSGNVHLEPGKRMSVLNQNHNMFDEHTVLETVMMGNKVLHKIKTEMDAL
                     YMDYTDENADRIGELQVQFEEMNGWNADSDAASMLSNLGITEENHYTLMADLEGKIKV
                     RVLLAQALFGNPDLLIMDEPTNDLDFETIAWLENFLANYENTVIVVSHDRHFLDAVCT
                     HISDIDFGKINHYSGNYTFWYESSQLAAKQRAQQNKKAEEKKQELEEFIRRFSANVAK
                     SKQATSRKKMISKLNISEIRPSSRRYPAIIFDQEREAGDQILNVQNLSASVDGEILFK
                     GVDLNMAKGDKIVLFSKDSRATTAFYEILNDNQKADTGIFDWGVTTTQAYLPAENHSF
                     FESDDSLVDWLRQWVKTEEERDEVNIRSFLGKMIFSGEEALKTCRVLSGGEKVRCMLS
                     RMMMQRANVLMLDEPTNHLDLESITAFNNSLKNFKGSVIFTTHDHEFSQTVGNRVVEL
                     TPKGAIDRYMTFDEYLDNEGVQELRTKMYS"
     gene            6059..7495
                     /locus_tag="PLQ70_04575"
     CDS             6059..7495
                     /locus_tag="PLQ70_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE33924.1"
                     /translation="MNSENSQTKWGQFIPLVTVFFFWGFVAASNDILIPVFKKAFDLT
                     QGQSQFVSIAFYISYTVGSLIYMGVSLALGKDLVNKIGYKNGLALGLVISALGTLLFY
                     PAANTGSYPLMLAGLFTVGLGFSLQQTVANPLAIALGPIKTGSQRLTLAGGINNFGTT
                     IGPLIVSFAIFGAAASGNTDMSIESVKIPYLILGLAFIIVAVLLKFSSLPEHPETIVE
                     TAEEATSTKTTALQYPQLLMGMLAIFLYVGVEVATAANLPAYMESKLGFAIGDIAPYV
                     SLYWASLMIGRWTGAVEAFTDDMSIQKILRFVAPYLAFGVFLIVNAIAKHDLTPFYVY
                     GLIILVLIAADMASKGNPARMLLIFSVLGAIALFTGMATSGMISVYAFTSVGLFCSTL
                     WPCIFTLAVSGLGKHTSQGSSFLIMMIMGGGIISWTQGFVSDSIGIQNSYIVGVLCFA
                     YLAFYAWKVSGILRAQGIDFDKKISGGH"
     gene            complement(7563..7733)
                     /locus_tag="PLQ70_04580"
     CDS             complement(7563..7733)
                     /locus_tag="PLQ70_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33925.1"
                     /translation="MFEFQQYLGFLLFLTVLTIGFWLMFFLVGFVSYWVGGATWEAYK
                     EKKAKKEAEQSV"
     gene            complement(7872..9065)
                     /locus_tag="PLQ70_04585"
     CDS             complement(7872..9065)
                     /locus_tag="PLQ70_04585"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010181311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HQE33926.1"
                     /translation="MKIVIINSGSSSIKYQLIEMPTKEVICSGMIDRIGLETSNFSYV
                     TNTVKVEETLPIANHKIGLNKIAQLLMDEKIGVIKSTSEIFAVGHRVVHGGSYFSDTA
                     LITTEVKEEIRELCDLAPLHNPAHLEGIIVAEEIFADAKQVVVFDTAFHQTMPAVAYK
                     YAIPNFLLTEHKIRAYGFHGTSHKYVSEKAIDYLKSVGNPYNNIITIHLGNGCSITAI
                     KDGKCIDHSMGFSPGNGLIMGTRSGDVDQAVIFYMINTLGYAPNEVNTILNKQSGMLG
                     LTGFSDLRDIESNAEQGNKECQLALDMNAYRIRKYIGSYVAALGGLDAIIFTAGIGEN
                     SSFIRKLVCTDMEFFGIELDNEKNEIRSKEIREINLPQSKAKILVIPTNEEIEIANQV
                     FELLS"
     gene            complement(9182..>10984)
                     /gene="pta"
                     /locus_tag="PLQ70_04590"
     CDS             complement(9182..>10984)
                     /gene="pta"
                     /locus_tag="PLQ70_04590"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HQE33927.1"
                     /translation="KQLEEQFDFVLVEGTSFTGEGAVIEMDMNVLIAKNLGIPAIIVG
                     SGVNKTLDELVDGMCLAYDSFKQKDVEVLAVIGNKVESQNIELVTSGLKKNLPDGVLI
                     NAIPMIPSLKNTTIQEIVKALDAKVLFGESYLNNQISSFSVGAMQLRNYLNHLKEDGL
                     VITPGDRADIILGALQANESKNYPSISGIVLTGNIIPEDTILKLIEGLSPVVPVIAVK
                     EGTYVISNKIGDIKPKIYANNKQKIQISIDTFEKYVDLDSLTEKFIKFEVEGMTPKMF
                     QYNLVKRAKQHRKHIVLPEGNDDRILIAAARLYLMDVVDITILGNTKQIISKSAELGL
                     DIDFTKVNLINPRKDKNYDDYVNTYYELRKEKKMTLEIAKDLMEDGSYFGTMMVYKGH
                     ADGMVSGAAHTTQHTILPALQFIKTKPNSSVVSSIFFMCLEDRVSIFGDCAINPNPTA
                     EQLAEIAISSADSSLAFGIEPKVAMLSYSSGSSGKGNEVDKVREATEIVKSKRPDLKI
                     EGPIQYDAAVDLEVGQSKMPNSEVAGHASVLVFPDLNTGNNTYKAVQRETGALAIGPM
                     LQGLNKPVNDLSRGCTVDDIINTVVITAIQAQGL"
BASE COUNT         3439 a         2018 c         1702 g         3825 t
ORIGIN      
        1 aacaattacc gattaaaata caaataaaat cgatgaaata cacaatatat ttttatatat
       61 atgtaattat cttacaaata aataaaattc gcaaattgta taacattaaa aaagggagat
      121 taaaatacgt tcttaaggtg caagtctatc tattttccaa aaataatcgt cttgcagctc
      181 atatctaatt ctgtcgtgaa gtctgttggg gcgtccttgc caaaattcaa ctgctacagg
      241 acgaattaaa taacctcccc aataattagg tcttggaatt tctttgcctt caaaatcgtg
      301 ttctaattgt ttcaaactct cttctaaaac gcttcgagag gcaataattt cactttgatt
      361 agaaactatt gcacccaatt tgcttcccgt tgggcgagat tcaaaataac catcagaaag
      421 attctccgaa atcttctcgg caattccttt tataataact tggcgttcta aagatgacca
      481 aaaaaaggaa agacaaacat tgggattatt ggcgatagcc ctaccctttt cagaattata
      541 attggtgtaa aaaataaatc cttcataagt aaattgcttc aaaagcacga ctctagactt
      601 tggaaatcca tccaatccaa aagtcgaaac cgtcatagca ttgacttcat ctacaccgcc
      661 aaaatcttcg acttcatgaa accatctgtt gaataaattg ataggatctt ctggaatatt
      721 ggtttccaaa agttcacttt tttcgtatga ttttctataa ttacttaaat cttccattgt
      781 ttaatttctt tagttgcaaa aataagcaaa cttatactca taaaagagtt tagcgcaaat
      841 gtattatctt gatatttgtc acgctgtaag cgtctaaatt atgttgctag attgtaattt
      901 gagacgctta cagcgtgaca attggaatga ttaaatcaaa actcaaaaca ttttccatct
      961 tctgctaaaa gcacattcga aaaaatggtt tcggcttgct ctttaaaaag ctcaatgctt
     1021 tcatatctag tagaataatg ccctaaaatc aattgtttga cgttggcttt caaagctatt
     1081 gtcgcagctt cttttgccgt agaatgcatc gttttgcctg ccaattcttc ctctgaatcc
     1141 aaaaaagtag attcgtggta taatatgtca acaccattaa taataggaat tatagcttca
     1201 ttataaaccg tatccgaaca aaaagcataa ctcaaaggca aaactggatc gaaggtcaat
     1261 ttatgatttt cgatcactcg tccgtcatcc agcgtgatgt cttttccgtt cttgatattt
     1321 tgataataac atttttcaat ttcgtagttt tgaacggcat ccaagttcaa ttttcgctcc
     1381 ctacgttttt cttgaaataa aaaaccattg gtgtaaactc gatgttttaa aggaattgtc
     1441 tttactatta ctttatcgtc ttcaaaaacc acttcacttt ctttggattc taattcgtga
     1501 aaaaacaagt cgtattttgt ccaagaatta gacaatcgca actgcaaatt gatgatttcc
     1561 ttgattcctt ttggcccata aatatgcaaa tcggtagttc tacccagcaa agcaaaagta
     1621 gaaatcaaac caatcaatcc aaaaaaatga tcgccgtgca aatgcgaaat aaatacatga
     1681 ttaattttcg aaaatttgat tttgtttttg cgcaactgta cttgagttcc ctcaccacaa
     1741 tcgattaaga ataatctgtt tctgatttct aaaacctgtg aagtaggatt ggtaaaagtt
     1801 cttggagttg cggcataaca gccgaggatg gttagattca ttttttagtg ttcagtcgca
     1861 gtaatcagta ttcagtgata attagctgaa aaaatctaaa atctgcaatc gttaatcttc
     1921 attcaaaaat ctaaaagccc aaatctcttt cgatttcttc catttctata atatcgtgcg
     1981 cttcttgaac cgatcgaacc acagccaatt tatgagaaat cgcattaaaa tcaaactctg
     2041 atgatactac aacaaatgat tttttgtttt ttttatgggt tttggaaaga ggcaaaaaac
     2101 tttcaatatc tttaatcgaa agagttttat gcattcgaat atcaacaata atattgcttt
     2161 tttggaatgt tttatattcg ttagtcaatt tcaaacgaaa tgaatccaaa tcaccttgag
     2221 tatctttgat agtcaccgtg tgtccttttt gttctacttt catatgttga aatttagggt
     2281 attgctaatt aacaaaagat atttattgtt tgaaaggtag taaaaaaata aagaattatt
     2341 gaatttttga agccaataaa taaattacag ccattcgaat ggcaacacca ttttctactt
     2401 gattcagtat gatggattgt ttagaatcgg caacatctga ggtaatttcc acaccacggt
     2461 tgatggggcc aggatgcata attaagattt ctttatccag tgaatctagc aaggttttgt
     2521 ctaatccgta ttgttgagca tattctcttg tagaaggaaa aaagttaaca tccattcttt
     2581 cgttttgaac acgaagcata ttagctacat cacaccattc caacgcctta cgtaaattag
     2641 gctcaaccgt aactccaagt gattcaatat gccttggaat caaggtattt ggtccacaaa
     2701 ctctaacttc ggcaccttgc atttgcaaag cgtagatatt agaaagagct actctggaat
     2761 gcaaaatatc tccgacaatg accacttttt tacccgcaac atcgcccagt ttttctctga
     2821 tagaataact atctagcaaa gcttgtgtag gatgttcgtg tgctccgtct ccagcattaa
     2881 cgatactggc ttttacattt tgcgacaaga aatgagctgc tccaggattt gaatgtcgca
     2941 ttaccaccat atctactttc atcgacagga tgttgtttac ggtatcaatt agagtctctc
     3001 cttttttgac cgaagattgt gctgccgaaa agctaatcac gtcagcagac aaccgttttt
     3061 gtgctaattc aaaagaaagt ttggttcggg tactgttttc gaaaaagatg ttggcaatgg
     3121 taatgtctcg aagtgaaggc acttttttga tgggacgatt gataacttct ttaaaatgat
     3181 cggcagtttc aaaaatgagg ttaatatctt tttcattgat atatttaatt cccaataaat
     3241 gatttacact taactcgctc attttctatt atttattaat taaataaaca gcatcttcgc
     3301 cgtcttgttc tttccaacac actaacactc tttcgtcatt gatagcatct acctgacgac
     3361 cacgataatc gggttgaata ggcaaatggc gactaaaacg acggtcgatt agtgtcaata
     3421 attcaatttc agatggtctt ccaaaagact gaatagcagt caatgctgac cgaatacttc
     3481 gacctgtata taaaacatcg tcaatgaaaa cgactttttt attttcgaca ataaaatcga
     3541 tttgggtttt attagcttcc aaaggcttgt tagttctgcg gaaatcatct ctaaagaaag
     3601 tgatgtccaa atagcccaaa gcaatttttg aaatattgta ttccttttcc aacatatctt
     3661 ttaatcgccg tgctaaaaaa ataccacgtg gctgaatacc aactagaata gtatccgaaa
     3721 aatcaagatg tttttcgact aactgacagg ccaaacgatg cagaatgata ttgacttcct
     3781 ttgaagtgag caatactttt tgactcataa tgaagtgttg tttgaaatgt aaagatatac
     3841 tattttttga aattttaaaa ttggtttatt tataaaaatt ttcataaaaa atcccgctgt
     3901 atgcaacggg ataaaatctt tactctatta tctatactct attctctttt aagaatacat
     3961 cttagttctt aattcctgaa cgccttcgtt atccaaatat tcgtcaaaag tcatatagcg
     4021 atcaatcgct ccttttggag tcaattcaac tactctattt ccaacagttt gagaaaactc
     4081 gtggtcatga gtggtaaaaa ttactgagcc tttaaagttt ttcaaagagt tattgaatgc
     4141 tgtaatcgac tctaaatcta agtggtttgt aggttcgtcc aacattaaaa cattggcacg
     4201 ctgcatcatc attcgagaca acatacaacg cactttttcg cctccagata aaactctaca
     4261 agttttcaag gcttcttctc cagagaaaat cattttcccc aagaagcttc taatattcac
     4321 ttcatcgcgt tcttcttcgg tttttaccca ttgacgcaac caatctacca atgaatcatc
     4381 actttcaaag aaagaatggt tttctgcagg caaataggct tgagttgtag tcactcccca
     4441 atcaaatatt cctgtatcgg ctttctgatt gtcgttcaaa atttcataaa aagcggttgt
     4501 agcacgagaa tctttcgaaa aaagtacaat tttatcgcct ttggccatat tcaaatcgac
     4561 acctttaaaa agaatttccc catcaaccga agcgcttagg ttttgtacat tcaaaatttg
     4621 atctcccgct tcgcgttctt ggtcaaaaat aatagctgga taacgacgac ttgacggtct
     4681 aatttctgaa atattcaatt tagaaatcat ttttttacga gaagtcgctt gtttggattt
     4741 ggcaacattc gcagaaaaac gacgaataaa ttcttctaat tcttgtttct tttcttcggc
     4801 ttttttgttt tgttgagccc tttgtttagc tgctaattga ctggattcat accaaaaagt
     4861 atagttccct gaataatgat taatttttcc aaaatctata tccgaaatat gagtacaaac
     4921 cgcatccaaa aagtgacggt cgtgagacac aacgattacc gtattttcat aattggccaa
     4981 gaaattttct agccaagcaa tggtttcaaa atccaaatca ttggtaggct catccataat
     5041 aagcaaatct ggatttccaa ataaggcttg agccaaaagc actcgtactt ttatttttcc
     5101 ttccaaatca gccattaaag tataatgatt ttcttctgtg attccaaggt ttgaaagcat
     5161 agaagcggca tcagaatcag cgttccatcc attcatttct tcaaattgca cttgcaactc
     5221 tcctattcta tcggcatttt catctgtata atccatatac agtgcgtcca tttcggtttt
     5281 aattttatgc aaaaccttat tccccatcat taccgtttca agaaccgtat gttcatcaaa
     5341 catattgtga ttttgattta aaaccgacat ccgttttcca ggttctaggt gaacatttcc
     5401 tgaagtagga tccatttcgc ctgaaataat ttttagaaat gtagattttc cagctccgtt
     5461 tgcgccaata actccgtaaa catttccgtg agtaaaaacc gtatttactt cgtcgaataa
     5521 gattcttttg ccaaactgaa ccgataaatt attgatagat aacataagtt gtttttataa
     5581 aattttgtgc aaaagtacaa aaaaatgtgc catttaaaat ccaaaataat gaacaaattg
     5641 aattatttca tttttcaata cccattctaa acggctataa aaatttctga ttaaaaaaat
     5701 taggcactta attttaacac ttgatttgct taatacattt gatttaaaac taattcattt
     5761 cataaaagta tcgtttgtat aaaaaaaaca gttccagaat catatacatg ttatcaaaaa
     5821 agctaatatg tccattattt tgaaggatac gtccatttta gattataaat aattactgta
     5881 tttttacctc ttaatcaagt gtgctttttg tattattatt tttcaaaaat acagttggtt
     5941 gaacaaatcg tgttgttggt ataatttcca taacatttga gctaaaatgt tttttatttg
     6001 tttctattca gtttatattt acagtaattt acactaatct aataaccatt tttaaattat
     6061 gaattcagaa aattcacaaa ccaaatgggg gcaatttatt ccccttgtaa ccgtattctt
     6121 tttttgggga tttgttgcag cgagtaacga tattttaatc ccagtattta aaaaagcatt
     6181 tgatttaaca caaggtcaaa gccagtttgt atctatcgct ttctacatct catacactgt
     6241 aggttcttta atatacatgg gagtttcttt agcattagga aaagatttag ttaataaaat
     6301 tgggtataaa aatggactag cattgggatt agtaatttcg gctttaggaa cattattatt
     6361 ttatcccgcg gccaatacgg gttcatatcc tttaatgttg gctggtttat ttaccgtggg
     6421 tttaggattt tcattgcaac agactgttgc caatccatta gccattgctt taggaccaat
     6481 aaaaacgggt tcacaacgtt tgacattagc gggaggaatc aataattttg gaacaaccat
     6541 tgggccactt attgtaagtt ttgccatttt tggagctgcc gcatcaggaa acacagatat
     6601 gagtattgaa agtgtaaaaa tcccttattt gattttaggt ttagccttta taattgtagc
     6661 cgttttattg aaattttcat ccttacctga acatcctgaa accattgtag aaactgctga
     6721 ggaagctact tctacaaaaa ctaccgcttt gcaataccca caattgttaa tgggaatgtt
     6781 agctatcttt ttatatgttg gtgttgaagt tgcaactgca gccaatttac cagcctatat
     6841 ggaatctaaa ttaggatttg ccattggtga tattgcccca tatgtttcat tgtattgggc
     6901 aagtttaatg attggtcgtt ggacaggagc tgttgaagct tttactgacg atatgtctat
     6961 tcaaaaaata ttacgtttcg tagcacctta tttggcgttt ggagttttct taatcgtaaa
     7021 tgccattgca aaacacgatt taacaccttt ctatgtttat ggattaatca ttttagtttt
     7081 aattgctgcc gatatggcta gtaaagggaa tccggctaga atgttactta ttttttctgt
     7141 attaggtgct attgcattat ttacaggaat ggctacttca ggaatgatta gtgtttatgc
     7201 ttttactagt gttggtttat tttgtagcac gctttggcca tgtattttta ctttggccgt
     7261 tagtggacta ggaaaacaca ccagccaagg aagtagtttc ttgattatga tgataatggg
     7321 tggtggaatt ataagttgga cccaaggatt tgtgtctgat agtatcggaa ttcaaaacag
     7381 ttatatagtt ggtgttttat gttttgctta tttggcgttt tatgcttgga aagtaagcgg
     7441 aattctgaga gctcaaggaa tagattttga taaaaaaatc agtggaggtc attaatatcc
     7501 aaaaaacatc ttaacaaaaa aaaggactca tttctgagtc ctttttttat ttagcaacaa
     7561 tcttatacag attgttctgc ttcttttttt gctttttttt ctttgtaagc ttcccaagtt
     7621 gctcctccta cccaatagga tacgaaacca accaagaaaa acattaacca aaatcctata
     7681 gtaagaacgg ttaagaaaag taaaaagcct aaatactgtt gaaattcaaa catgttttcc
     7741 ggaattttga atgtagctac aaatgtatgt ttaatatttt ttcttagcaa taaaaattga
     7801 tactatttta tttattacac ttatttattc cttttcaaac gcctttaatg aaattcaggt
     7861 tgactcgttg attacgatag caattcaaat acttgattgg caatttctat ttcttcatta
     7921 gtaggaatta ctaaaatttt agctttggat tgtggtaaat tgatttctct aatttctttt
     7981 gaacggattt catttttttc gttatctaat tcaattccga aaaactccat atctgtacaa
     8041 actaattttc gaatgaaaga cgaattctca ccaattccag cagtaaaaat aatggcgtct
     8101 aaacctccta aggccgctac ataagaacca atatatttcc tgattcggta ggcattcata
     8161 tctaaagcca attggcattc tttattgcct tgctcagcat tcgattcgat atctctcaaa
     8221 tcgctgaaac ctgttagccc taacattcca ctttgtttgt tcaaaatcgt gttcacttcg
     8281 tttggagcat atcctaaggt attgatcata taaaatataa ctgcttgatc aacatcacca
     8341 cttctggttc ccataatcaa tccatttcca ggactgaaac ccatagaatg atcgatacat
     8401 tttccgtctt ttattgccgt aatactacaa ccatttccta aatggatagt tatgatatta
     8461 ttatacggat taccaactga cttcaaataa tcaatggctt tttccgatac gtatttatga
     8521 gaagttccgt gaaaaccata agcacgaatt ttatgttcgg tcaataagaa attaggaatg
     8581 gcatatttat aggccacagc tggcatagtt tgatgaaaag cagtatcaaa aacgactact
     8641 tgcttagcat cagcaaaaat ttcttcggct acaataatac cttctaaatg cgcgggattg
     8701 tgcagaggag ctaaatcaca aagttctcta atttcttctt ttacttctgt agtaatcaat
     8761 gcagtatctg aaaaataact acctccgtgc accacacgat ggcctactgc aaatatctcg
     8821 ctagtacttt tgattactcc aattttttca tccatcaata attgagcaat tttgttgaga
     8881 ccaattttat gattggctat aggcaacgtt tcttcaactt tgacggtatt ggtcacataa
     8941 ctaaaatttg aagtttccaa accgattcta tcaatcatac ccgaacaaat cacttctttt
     9001 gttggcattt caattaattg atattttata gaagaacttc ccgaattaat aataactatt
     9061 ttcatctttt actttttttt ctttttccta agatattttt ttttataatg atcaaaagta
     9121 tatttttttt gcaaatatac ctcaatgtaa tcgattaacc tattcaatta acaaaagggt
     9181 tttacaaccc ttgtgcttga atagctgtaa taacaaccgt gttgattata tcatcaacgg
     9241 tacaaccacg actcaaatcg tttacaggtt tgtttaatcc ttgtaacatt ggaccaattg
     9301 ccaaagctcc tgtttctctt tgaacggctt tgtatgtgtt atttcctgta tttaaatcag
     9361 ggaaaaccaa tacactagca tgtcctgcta cttcagaatt tggcattttg ctttgtccaa
     9421 cttctaaatc aacggctgca tcatactgaa ttggaccttc aattttcaaa tcagggcgtt
     9481 tgctctttac aatttcggtt gcttctctaa ctttatcaac ttcattacct tttccagatg
     9541 aacctgatga atacgatagc atagctactt ttggttcgat tccgaatgcc aaacttgaat
     9601 cggccgatga aattgcaatt tctgctaatt gttcggctgt tgggtttgga ttaatcgcac
     9661 aatctccaaa gatagaaact ctatcttcta aacacataaa gaatatggac gaaactactg
     9721 aagaattagg ttttgttttg atgaattgca aagctggcaa aatagtatgt tgggtggtat
     9781 gagccgcacc cgaaaccatt ccgtctgcat gacctttata aaccatcatt gtgccaaaat
     9841 atgaaccgtc ttccattaaa tctttggcaa tttccaaagt cattttcttt tcttttctca
     9901 gctcataata ggtattcaca taatcatcat aatttttatc ttttctagga ttgattaggt
     9961 tcactttagt aaaatcgata tctaaaccta attcggccga tttgctgata atttgtttag
    10021 tgtttcctaa gatagtaata tcgaccacat ccatcaagta cagtcgggct gctgcaataa
    10081 ggattctatc gtcgtttcct tccggtaaaa caatatgttt tcgatgttgt ttggctcttt
    10141 ttactaagtt gtattggaac atctttggag tcattccttc tacttcaaac ttgatgaatt
    10201 tctcagtcaa actatctaaa tccacatatt tttcgaaggt atctatcgat atttgaattt
    10261 tctgtttatt gttggcataa atcttaggtt tgatgtcgcc tattttatta gaaatcacat
    10321 aagtaccttc ttttactgca ataactggta caactggcga aagaccttca attaatttaa
    10381 ggattgtgtc ttctggaata atattccccg tcaaaacgat tccagaaatc gaaggataat
    10441 ttttagattc attagcttgt aaagcaccca aaataatatc ggctctatca cccggagtaa
    10501 ttactaatcc gtcttctttt agatgattta aataatttct taattgcatc gctcctacgc
    10561 taaaactaga aatttgatta ttcaaataag attctccaaa caatacttta gcatctaaag
    10621 cttttacaat ttcttgaatt gtggtgttct ttaaactcgg aatcatcgga atagcattaa
    10681 tcaaaactcc gtctggtaaa tttttcttca aaccagaggt aaccaattct atgttttgtg
    10741 actcaacctt atttccaata acggccaaaa cttcaacatc cttttgttta aatgaatcat
    10801 aggccaaaca cattccatca accaattcgt ctaaagtttt gttcactcct gagccaacaa
    10861 taattgctgg aattccaaga tttttagcaa taagtacatt catatccatt tcgataactg
    10921 caccttcacc cgtaaaacta gtcccttcga ccaaaacaaa atcaaattgt tcttctagtt
    10981 gttt
//
LOCUS       DAOSNN010000059        10932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_5986_length_10932_cov_4.831203, whole genome shotgun sequence.
ACCESSION   DAOSNN010000059 DAOSNN010000000
VERSION     DAOSNN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5986_length_10932_cov_4.831203"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PLQ70_04595"
     CDS             <1..1182
                     /locus_tag="PLQ70_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HQE33928.1"
                     /translation="KSETIQSMEAFNQGWGRILYRTQLPESKTSFKLKITELRDWATI
                     YVNGKLIGNLDRRKDENTIQIPAVKKGDVLDILVEAMGRVNYGKTIIDRKGITEKVEL
                     LSATHSTNLTNWEVFNFPVDYDFQKNLKFKKEKANGPAWYKATFDLKSTGDTYINMST
                     WGKGMVWINGYNLGRYWKVGPQQTLYMPGCWLKKGKNEIIILDLETPTATQISGVTTP
                     VLDKIVIDESLLHRKKGETLDLSAEAPVNSGSFIAGQGWKEVIFDKESEGQYFCFEAL
                     SSQNLKDQSSSMAEFELIGVDGLPINRSKWKIIYADSEEISVGNYSAEKIFDQQESTF
                     WSTAWTVSKTPHPHHVVVNMDEIVKIKGFKYLPRTDKSTNGNVNSYRFFIKSSLFSIK
                     K"
     gene            1196..4099
                     /locus_tag="PLQ70_04600"
     CDS             1196..4099
                     /locus_tag="PLQ70_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HQE33929.1"
                     /translation="MNRNSYICLLFLALFSLSSFNSKAQVINLKGNWLFQMDQNDEGV
                     TAKWYNKKLADVIHLPGSMAENLKGDEITLKTKWTGSIYDSSYYFVPRLKKFREPGNI
                     HIPFWLTPAKHYVGVAWYQKEVNIPKNWKGQRIVLHLERAHIETRVWVNDQEVGLQNS
                     LVAPHNYDLSKYLVPGKQRISIRIDNQIKKINVGPDSHSVTDHTQGNWNGIIGKMELE
                     AASPVYIDDVQIYPDIKNRKARVKITVNNSSPNDFNGKITLSAKSFNSQDSDKTQEIA
                     SNINVAKLGENTLELELPFGNGMLTWDEFNPALYHLEVKLDSKEIKNTKTVQFGMREF
                     KINGNQFLINGNPVFLRGTVHNCEFPLTGYPATTVEAWEKILQKIKSYGLNHVRFHSW
                     CPPEAAFIAADKVGLYLQPEGPSWPNHGPKIGLGQPVDKFLYDETLRMAKEYGNYASF
                     TMLSAGNEPAGNQVKYLNEFVDFWKAKDNRRVYTGMSVGGSWPVVPNAEYQSRGGVRG
                     LKWEKMPETESDFSSGIAPFKVPFVSHEIGQYCVFPNFDEIQKYTGVYRAKNFEMFRE
                     DLDDHHMGNQSKDFLIASGKLQVLCYKNEIEKMLRTPGYGGFQALGLQDFPGQGTALV
                     GVLDAFFQEKGYVTAAEYSRFCNSTVPLAKIPKFAYTNAETFKAEIVLFHSGKTPLKN
                     AIISWTIQNEKGNILSSGSFDPKTFENGNGQFVGNLSFPLVDIKEASKLKLEVKVNDT
                     AFVNDWNFWVYPDQKVEVSPAIYYTTTLDEKAKEVLDNGGKVFLNASGKVVKGKEVEM
                     HFLPVFWNTSWFKMRPPHVTGMLIQDKSPAFSNFPTSFHSDLQWWEIQNRSQVMNLED
                     FPVDFRPLIQPIDTWFMNRRLALVFEAQVGKGKIIVSSANLSPDLENKPAAKQLFLSL
                     QEYMNSDQFSPKNSLTLDVIQDLFLSPSKEIFNTYTKDSPDELKPNSNQNKVK"
     gene            4117..7101
                     /locus_tag="PLQ70_04605"
     CDS             4117..7101
                     /locus_tag="PLQ70_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HQE33930.1"
                     /translation="MKNSRFYILIFFLIAVVSVNAQKNGSDREKYNFNYGWKLNIGDV
                     EEAKQADFNDKKWKDVNLPYAWNQSEAFRLDITQLSTGIAWYRKTFQLPKDSNNKKVF
                     VEFEGVRQMGEIYINGTFIGRHENGVMAFGFDLTPYVKPYPQQNVIAVKTDNDWKYKE
                     KSSGSGFQWNNNNFNANYGGIPKNVFLHLKPKVYQTLPLYSTLGTTGTYVYATNFDIA
                     NKKAVVNVASQVKNELSKSITTQLKVSVQDREGKLIATFVGDSFAIAPNETKDLTAMK
                     ALDNLNFWSWGYGYLYTVTSELLVDTKSVEKVTIQTGFRKTKFEKGMVYLNDRVIMMK
                     GYAQRTSNEWPSVGMSVPAWLSDYSNSLMIKSNANLVRWMHVTPWKQDIESCDRVGLI
                     QAMPAGDSEADVQGRRWEQRTELMRDAIIYNRNNPSIIFYESGNESISEDHMKEMLAI
                     KNTFDPQGGRAIGSREMLDSKVAEYGGEMLYINKSARIPFWATEYSRDEGLRKYWDEF
                     TPPFHKEGSGGTAHTNVNGSKVADASAYNHNQDTHAIEDVVRWFDYWEMRPGTGERVN
                     SGGVNIIFSDSNTHYRGQENYRRSGEVDAMRIPKDNFYAHQVIWNGWVDTDTPDLHIV
                     GHWNYENGVTKDITVISTADVVELFVNNESLGKGIQDKRFLFTFKKVSFKPGIIKAIG
                     YDKTGKKVCENQIQTAGKPFGIKLSTIERPGGTLANANDLALVQVEVVDEKGNRCPTA
                     LNEINFDVQGQGTFIGGIAQGPDNYIGSKKLPVECGVNRIFVKSIDIAGNIKILATSD
                     SLKSDAITIKTIKTDIEKVSNTLPSENLSSFTEKGPTPATPSFSKTRTAVAIIAANTD
                     SNQADAVKSYDDNELTQWSYGKTGKAMIEYELEREANINQVVLKLAGWRSKKYPIRIS
                     IDNKKVFEGLTPLSLGYVTLDFEPTKGKKIQIELMATAKDSDAINLVEITGKVDQAGI
                     NKEGKVIEQLPIVEVEFYEPIR"
     gene            7577..10306
                     /locus_tag="PLQ70_04610"
     CDS             7577..10306
                     /locus_tag="PLQ70_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006989501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQE33931.1"
                     /translation="MLRKLSFIISICYLFLLCSCNQTTKDGKNISIGFSQSISEDDSW
                     RKSMDNTMTVEASLHPEIDLTIYNANNSTKKQIQDIERMIDNHMDVIIVAPYGSDSIV
                     PVIEKAAKKGIPVIIVDRKVNTSNYTSFLGADNVEVGHIAGKYIVSMSKAKATILEIK
                     ATTAISPSVERSLGFKQIIDQYPNIKKKSISAPDLAALNDKLSKVLDSMPNLDYIFAF
                     NDIIALQAWDVAKQKGREKSIKFIGIDGLNDPNGGIQAVKDGKLTATILYPTGGSEAI
                     KLAIKLANKEVVAKNNTLNTILIDSLNADIMSNQFDRITIQQSNIELQQKAIKEQEKK
                     YLTLYNLIKLLSFLLVVILCLGLFSIYSVVSIKKKKKLLEETNSKIIQQRNEIEKFVE
                     KLEESNESKINFFTGISHEFKTPLTLILSSVESLHDEFKNKSHSVKKDLDIMYNNSLR
                     LLRLINQLLDFRKVEDKKFTLKLSKNNLWKFSVNMFKEFKREAKKRKINYTIDTDNEE
                     LEVYFDANLMDKVYYNLLSNCFKFTPDGGRIEIKISQEKNKGLVKINFKDSGIGIPEK
                     EINQIFSPFFQGSNNYRNGSGIGLNLSKNFVDLHEGSIEVNSVNGAEFSVYLKLGDNH
                     IDVSKIIKDSDYVSDSENEYEYLETELTPSTESLITENKHSILIIEDNVDLLDFIATK
                     LKEDYSVTRCNGTKAIEMALELMPDIVVCDLNLPEKNGFEICEALKTNMITSHIPVII
                     LTATDDDNTYIKSLEVGADLFLTKPFNLKVLKQSIKGLLFNREKLRYYYTNNIDKIES
                     DGLSLIEKEFLNKINLILEKHLDDSSFSVEELAGKLSISRVQLYRKVKAVLGIGVSDY
                     INNFRLEKAKELLTTSTMNISEIAYSCGFASPNYFSTAFKTKYDMTPKDFRNN"
     gene            10504..>10932
                     /locus_tag="PLQ70_04615"
     CDS             10504..>10932
                     /locus_tag="PLQ70_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33932.1"
                     /translation="MKKCIPIILSGILLIGCKSQNDPSPTPTPIPPSNVATELLSFNF
                     NETSGQSLSETKTNASFTINGPSGSAERIGGVESNALRVNGFYGWATGNASATYPTSK
                     VCVSGWIAPSAFPVQRKDLDPITENTNAAIFSNVNTSTSAG"
BASE COUNT         3812 a         1718 c         2131 g         3271 t
ORIGIN      
        1 aaatcggaaa ccatacaatc gatggaagcc tttaatcaag gttggggacg cattttatat
       61 cgcacccaac tgccagaatc caaaacatcg tttaaattga aaattaccga gttgcgggat
      121 tgggccacta tttatgtcaa tggaaaatta attggaaact tggatcgtcg caaagacgaa
      181 aacactatcc agattcctgc tgtaaaaaaa ggcgatgttc tagatatttt agtagaagct
      241 atgggacgtg tcaattacgg aaaaaccatt attgaccgaa aaggaattac tgagaaagtc
      301 gaattgctta gcgctaccca ttctaccaac ttgacgaact gggaggtttt caattttccg
      361 gtggattatg attttcagaa aaatttgaaa ttcaaaaaag aaaaagcaaa cggaccagct
      421 tggtacaaag ccacttttga tttgaaatct actggtgata cctatatcaa tatgagtact
      481 tggggcaaag gaatggtatg gataaatgga tataatttag gtcggtattg gaaagttggt
      541 cctcaacaaa ccctgtatat gccgggatgt tggttgaaaa aaggaaaaaa tgaaatcata
      601 attctcgatt tagaaacacc cacagcaact caaatttcag gagttacaac tcctgtttta
      661 gacaaaatag tgatagacga atcgcttttg caccgaaaaa aaggagaaac tctcgatctt
      721 tctgctgaag ctcctgtcaa ttctggctcg tttattgcag ggcaaggttg gaaagaagtg
      781 atttttgata aagaaagtga agggcagtat ttttgttttg aggcattaag ttctcaaaat
      841 ctaaaagacc aaagtagcag tatggctgaa tttgaattaa ttggcgtaga tggattgccg
      901 attaatcgtt ctaaatggaa aataatttat gcagacagcg aagaaatttc agtaggaaac
      961 tattcggccg aaaaaatatt cgaccaacag gaatctactt tttggtccac ggcttggacg
     1021 gtttcaaaaa cgccgcatcc ccatcatgtt gttgtcaata tggatgaaat tgtaaaaatt
     1081 aaaggcttta aatatttacc cagaaccgat aaaagtacca atggaaatgt gaattcctat
     1141 cgtttcttta ttaaatcatc cttgttttct attaagaaat aaaaaaaata acctaatgaa
     1201 tagaaatagt tatatctgct tgcttttttt agcattattt tctttaagca gtttcaatag
     1261 taaggctcaa gtaattaatt tgaaaggcaa ttggcttttt caaatggatc aaaatgacga
     1321 aggtgtcaca gcaaaatggt acaacaaaaa attggcagat gtaattcatc tgccaggttc
     1381 aatggccgaa aacttgaaag gcgatgaaat tactttgaaa accaaatgga caggctctat
     1441 ttatgacagt tcttactatt ttgtacctcg attaaaaaag tttcgcgagc ctggaaacat
     1501 acatattccc ttttggttga ctcctgcaaa acattatgtt ggcgtggctt ggtatcaaaa
     1561 agaagtaaac attcctaaga attggaaagg gcaacgaata gtgctccatt tagaaagagc
     1621 ccatatcgaa actagggttt gggtaaacga tcaggaagta ggtttgcaaa actcactcgt
     1681 tgctcctcac aactatgatt tgagtaaata tcttgttccc ggaaagcagc ggatttctat
     1741 tagaattgac aatcaaatca aaaaaataaa tgtgggacca gattcccata gcgtcaccga
     1801 tcatactcaa ggaaattgga atggtattat cggaaaaatg gagttggagg ctgcttctcc
     1861 agtatatata gacgatgtac agatttatcc tgacataaaa aatcgaaaag ctcgggtaaa
     1921 aatcacggtc aataatagta gtccaaatga ttttaacgga aaaattacgc tctctgccaa
     1981 aagtttcaat tcacaggatt cagacaaaac ccaagagatt gcaagcaata tcaatgtagc
     2041 taaattagga gagaatacac tcgaactcga gctccctttt ggaaatggga tgctaacctg
     2101 ggacgaattt aaccccgctt tgtatcattt agaagtcaag ttggattcta aagaaataaa
     2161 aaacactaaa accgttcagt ttggaatgcg tgaattcaag ataaatggca accagtttct
     2221 gataaacggt aatcctgttt ttcttcgtgg aacggttcat aattgtgaat ttcctttaac
     2281 gggttatcca gccactacgg tcgaagcttg ggagaagatt ttgcaaaaaa ttaaatcata
     2341 cggattgaac cacgttcgtt ttcattcttg gtgtccgccc gaagctgcct ttatcgctgc
     2401 cgataaagtg gggctttatt tgcaacccga agggccaagt tggcccaatc acggtccaaa
     2461 aataggattg gggcagcctg tagataaatt tttgtatgat gaaacgctga gaatggcaaa
     2521 ggaatacggc aattacgctt cttttacaat gttgtctgct gggaatgagc ctgcaggaaa
     2581 ccaagtgaaa tacttgaatg aatttgttga tttttggaaa gcaaaagaca accgaagagt
     2641 ttataccgga atgtcagttg gtggtagctg gccagttgtt cccaatgctg aatatcaatc
     2701 tcgtggggga gttagaggtt tgaaatggga aaaaatgcct gaaaccgaat ctgattttag
     2761 ctccggaatt gcaccattta aagttccttt tgtgtcacac gaaattggtc aatattgtgt
     2821 ttttccaaat ttcgatgaaa ttcaaaaata cacaggagtt tacagagcca aaaatttcga
     2881 aatgttccgc gaagatttag atgatcacca tatgggaaat caaagcaaag attttttgat
     2941 tgcctctgga aagttacagg tgttgtgtta taaaaacgaa atcgaaaaaa tgttgagaac
     3001 gcctggttac ggaggttttc aagcattagg attacaggat tttccgggtc aaggaacggc
     3061 tttagtgggt gttttggatg ctttttttca agaaaaagga tatgtaactg ctgctgaata
     3121 cagtcgattt tgtaattcta cagttccttt ggccaagatt ccaaagtttg catataccaa
     3181 tgctgaaacc tttaaggcag aaattgtgct gtttcattca ggaaaaacgc cattaaaaaa
     3241 tgctataatt agttggacga ttcaaaacga aaaaggaaat attctttctt caggaagttt
     3301 tgatcctaaa actttcgaaa atggaaatgg tcaattcgtt ggaaatctta gttttccatt
     3361 agtcgatatt aaagaagctt cgaaattaaa attggaagta aaagtcaacg atactgcttt
     3421 tgtcaacgac tggaattttt gggtgtatcc tgatcaaaaa gtggaagttt ccccagcgat
     3481 ttattacacg acaactttgg acgaaaaagc caaagaggtt ttggataatg gtggaaaagt
     3541 atttttgaat gcctcaggaa aagtggtaaa aggtaaagaa gtggaaatgc actttttgcc
     3601 tgttttttgg aatacctctt ggttcaaaat gcgtccgcct cacgttacag gaatgctaat
     3661 tcaggataaa agtcctgctt tttccaattt cccaacaagt ttccactctg atttgcaatg
     3721 gtgggaaatt caaaatcgct cgcaagtaat gaatctcgaa gattttccgg tagatttccg
     3781 acctttaatt caacctattg atacttggtt tatgaatcgc aggttggctt tagtatttga
     3841 agcccaagta ggtaaaggga aaatcattgt aagtagtgcc aatttgagtc cagatttgga
     3901 aaacaaacca gctgcaaaac aattgttttt atcgcttcaa gagtatatga attcagatca
     3961 atttagtccc aaaaacagct tgactttgga tgtaatccag gatctatttc taagcccatc
     4021 aaaagaaata tttaatacct atacgaaaga tagccctgat gaattgaaac ccaattcgaa
     4081 tcaaaataaa gtaaaataat taattaaaaa tcaataatga aaaatagtcg tttttatatt
     4141 ttgattttct tcctaattgc tgttgtttca gtaaatgcgc agaaaaacgg atcagataga
     4201 gaaaaataca acttcaatta cggttggaaa ttaaatattg gcgatgttga agaagctaaa
     4261 caagctgatt tcaatgacaa gaaatggaag gatgtaaatc ttccgtatgc ttggaatcaa
     4321 tctgaagctt ttcgtttaga tattacacaa ttatctacag gaattgcttg gtatcgcaag
     4381 acctttcagc ttcctaaaga ttcgaacaat aaaaaagttt ttgtcgagtt tgaaggagtg
     4441 cgtcaaatgg gagaaattta tatcaatgga acttttattg gccgacatga aaatggagtt
     4501 atggcttttg gttttgactt gactccttat gtaaaacctt atcctcagca aaatgtgata
     4561 gccgtaaaaa ccgataatga ttggaagtat aaagagaaat catcaggttc tggatttcaa
     4621 tggaacaata ataactttaa tgccaattat ggtggaattc ccaaaaatgt atttctacat
     4681 ttaaaaccca aagtgtatca aacactgccg ttgtattcta ctttgggcac aacaggaact
     4741 tatgtttatg ccaccaattt tgatattgca aacaaaaagg cagttgttaa tgtggcttcg
     4801 caagtaaaaa atgaactttc taaatcgatt actactcaat tgaaagtaag tgttcaagat
     4861 agggaaggaa aactgatagc tacttttgtt ggcgattctt tcgctattgc tccaaatgaa
     4921 actaaggatt taacagctat gaaagcttta gataatttga atttctggag ttggggctac
     4981 ggctatttat atacagttac ttcggaatta ttggtcgata ctaaatcagt cgaaaaagtg
     5041 actattcaaa cgggatttcg aaaaactaaa ttcgaaaaag ggatggtgta tttgaacgat
     5101 cgtgtcatta tgatgaaagg ctatgcacag cgaaccagca acgaatggcc gtctgtagga
     5161 atgtcagtac cagcttggtt aagcgattat agcaatagtt tgatgataaa aagtaatgca
     5221 aatttagtgc gatggatgca cgttacacct tggaagcagg atattgaatc gtgtgaccga
     5281 gtgggtttga ttcaagccat gccagcagga gattcagaag ccgatgttca gggtcgccga
     5341 tgggaacaac gcactgaatt aatgagagat gccatcattt acaatcgcaa taatcccagt
     5401 attatttttt acgaatctgg aaacgaaagc atctcggagg atcatatgaa agaaatgttg
     5461 gctatcaaaa acacattcga cccacaagga ggtcgcgcta ttggaagcag agaaatgctc
     5521 gacagcaaag tggctgaata tggtggagaa atgttgtaca tcaacaaaag tgctcgcatt
     5581 ccattttggg ctacagaata cagtagagat gaaggcttac gcaaatattg ggacgaattc
     5641 acgccacctt ttcacaaaga aggttcagga ggaacggctc atactaatgt caatggtagt
     5701 aaagtagccg atgctagcgc ttataatcac aatcaagata cacacgctat agaagatgtg
     5761 gtacggtggt ttgattattg ggaaatgcgc ccgggaacag gagaacgcgt gaattctggc
     5821 ggagtgaata ttatttttag tgattcgaat acgcattaca gagggcaaga aaattatcgc
     5881 agaagcggag aagtggacgc catgcgaatc ccaaaagaca atttctatgc tcatcaagtg
     5941 atttggaacg gatgggtcga taccgataca cccgatttgc atatcgtagg acattggaat
     6001 tatgaaaatg gtgtgaccaa agatattacc gtaatttcaa cagcagatgt agtagaatta
     6061 tttgtaaata acgaaagcct tggcaaaggc atacaagata aaagattctt attcactttc
     6121 aaaaaggtat cctttaaacc tggaattatc aaagccattg gttatgataa aacgggtaaa
     6181 aaagtttgtg aaaatcaaat tcaaaccgct ggaaaaccct ttggaattaa gttgagtacc
     6241 atcgaaagac caggagggac actagctaat gctaatgatt tggctttggt tcaagtagaa
     6301 gtagtggatg aaaaaggcaa tcgttgtcca acggcattaa acgaaatcaa ttttgatgtg
     6361 caaggacaag ggacttttat tggaggaata gcccaaggac ccgataatta tattggaagc
     6421 aaaaaacttc cagtagaatg tggtgtcaat cgaatcttcg ttaaatcaat cgatattgca
     6481 gggaatatta aaattttggc tacttcagat tctttaaaaa gtgatgcaat tactataaaa
     6541 actatcaaaa ccgatattga aaaggtatcg aatacgttgc caagcgaaaa tttatcatca
     6601 tttacagaaa aaggacccac tccggcaaca ccatctttct ccaaaactcg tactgctgtt
     6661 gctattatcg cagccaatac tgattctaat caggcagatg ctgtaaaaag ttatgatgac
     6721 aatgaattga cgcaatggtc ttatggaaaa actggcaaag cgatgattga atatgaatta
     6781 gagcgtgagg ccaatatcaa tcaagtggta ctgaagcttg ctggatggag aagtaaaaaa
     6841 tatccgattc ggatttcaat cgacaataaa aaagtgtttg aaggattaac tcctcttagt
     6901 ttgggatatg taaccctcga ttttgaacca actaaaggca aaaaaattca aattgaatta
     6961 atggcaacag ccaaagatag tgacgcaatt aacttagtcg aaattaccgg aaaagtagat
     7021 caagcgggaa taaataaaga aggcaaagtc atagaacaat tgcctatagt agaagttgag
     7081 ttttatgaac ccattcgata gtgaatttta aaaaagaagg aggttgtctt tttgagacaa
     7141 cctccttctt tttgtaattt ggtttaaatc gctatttgtt gggttaatta agcgaaatta
     7201 tttttgtctg tacttgaaga agttaagcct gaaattagtg aatttggaat agatgaagtg
     7261 attcctttgt agtctataat gaaagaagga cttgcgaaaa acgtaagtcc ttgtgttttt
     7321 tattgttcaa acataatgat tttgaataca aaaaaatcaa attaatttag ttgttcaaat
     7381 tgctcaatta acatttatag attgaatatt tttactgtag attgcttttc tatatattta
     7441 cattgtttta tataattaat ttaatttcgt actcaaaata attaatagtt ttgtaaagta
     7501 ctgcttcgtt tcaataataa ataatatctt gtattttttg atgttatttt aatcaattat
     7561 tttaataata aactcaatgc taagaaaact tagttttatc atttcaattt gttatttatt
     7621 tttattatgt tcttgtaatc aaactacaaa ggatgggaag aatatttcaa ttggattttc
     7681 acaaagtatc tctgaggacg atagttggag aaaatctatg gataatacaa tgacggttga
     7741 agcttcacta cacccagaaa ttgacttaac catttacaat gcaaataata gtactaaaaa
     7801 gcagattcaa gacattgaaa gaatgattga taatcatatg gatgtaataa tagtagcacc
     7861 ctatggttca gattctatag ttcctgtaat tgaaaaagcc gccaaaaaag gaatcccagt
     7921 aattatagtg gatagaaaag tcaatacttc caattacact tcttttttag gagccgacaa
     7981 tgttgaagtt ggacatattg ctggaaaata tattgtttca atgtctaaag ctaaggcaac
     8041 tattcttgaa attaaggcta cgactgctat ttcaccaagt gtagaaagaa gtttaggctt
     8101 caaacaaatt atcgatcaat atcccaacat aaaaaagaaa agtatatctg ctccagattt
     8161 agcagctcta aatgataaat tgagcaaggt tttggatagt atgccaaatt tagattacat
     8221 ctttgctttc aatgacatca ttgcccttca agcttgggat gtggccaaac aaaaagggag
     8281 agaaaagagc ataaaattta ttggaattga cggattgaat gatccaaatg gaggaattca
     8341 agctgttaaa gacggcaagc taacggcaac cattttatac ccaacaggag gaagcgaagc
     8401 gattaagttg gcaatcaagt tggcaaacaa agaagtagtt gccaagaata acacgctaaa
     8461 taccattttg attgactctt tgaatgccga cataatgagc aatcaatttg atagaattac
     8521 cattcaacaa tcaaatattg aattacaaca aaaagcgatt aaagaacagg aaaaaaaata
     8581 cttgaccttg tataatttaa tcaaattatt gagttttctt ttagtggtaa ttctatgttt
     8641 gggacttttt agtatttatt ccgtggtttc cataaaaaag aaaaagaagt tgttagaaga
     8701 aacaaactca aaaatcattc aacaaagaaa tgaaattgaa aaatttgtgg agaaattgga
     8761 agaaagcaat gaatccaaaa tcaatttttt tacgggcata tcccacgaat ttaaaacccc
     8821 attaactcta attttaagtt ccgttgaatc tttgcacgat gagtttaaaa acaaaagtca
     8881 ttctgttaaa aaagatttgg acattatgta caataattct ttgagattac ttcgattgat
     8941 taatcaattg ttggatttta gaaaggtcga ggataaaaaa ttcactctga aactatccaa
     9001 aaataatctt tggaaatttt cggttaatat gtttaaggaa ttcaaaagag aggctaaaaa
     9061 aagaaaaata aactacacca ttgacactga taacgaagaa ttggaagttt attttgatgc
     9121 caatttgatg gacaaggtgt attataatct cttgtctaat tgttttaaat tcactcctga
     9181 tggaggtcga atagaaatca aaattagtca agaaaagaac aaagggcttg ttaaaattaa
     9241 ttttaaagat tcaggtattg gaattcctga aaaggaaata aatcaaattt tcagtccctt
     9301 tttccaaggt tccaacaact atagaaatgg ttcgggcatt ggattgaatt tatccaagaa
     9361 ttttgttgat ttacacgaag gctccatcga agtaaactct gttaatggtg ccgaattttc
     9421 tgtttattta aagttgggag acaaccatat tgatgtgtca aaaatcataa aggacagtga
     9481 ttatgtttca gatagtgaaa atgaatacga gtatttggaa acggaattga cacccagtac
     9541 ggaatcccta attactgaaa acaaacattc tattttaatt atcgaggaca atgttgattt
     9601 actggatttt atcgcaacaa aattaaaaga agattattcg gttactagat gcaatggaac
     9661 caaagctata gaaatggcct tggaattaat gccggatatt gtagtttgtg atttaaattt
     9721 accggaaaag aatggttttg aaatttgcga agctttaaaa accaatatga ttacctcgca
     9781 tattccggta attattttaa cggcaacaga tgatgacaat acctatataa aatcattgga
     9841 agtaggagca gatttgtttt tgaccaaacc tttcaatcta aaagtcttga agcaatccat
     9901 caaaggcttg ctgttcaaca gggaaaagtt gcgctattat tataccaaca atatagataa
     9961 aattgaaagc gacggactca gcttgattga aaaagagttt ttgaacaaaa ttaatttgat
    10021 tttggaaaaa catcttgatg attcctcttt ctctgttgaa gaacttgccg gtaaattaag
    10081 tatttcacgt gttcagctct atagaaaagt aaaagcggtt ttaggcattg gggttagtga
    10141 ttatatcaat aatttcagac tggagaaagc aaaagagttg ttgacgacat caacgatgaa
    10201 tatttcggag attgcgtatt cttgtggttt tgcttctccc aattatttct caactgcctt
    10261 taaaacgaaa tacgatatga cgccaaaaga ctttagaaac aattaattgt actataacga
    10321 tgtaacattt ttatacatat tgtaatttgg agtacttttt ttaattgatt tctttgattt
    10381 ttttattctt aaagccttgt attaataagg gttagtgttt atttataaat aattaactag
    10441 gtgtaacaat acttaacttg ttcacggttc cattatagta attttatcta aaattattat
    10501 aaaatgaaaa agtgtatccc aattatccta tcaggaattc tactcattgg atgtaaatct
    10561 caaaatgacc caagtccaac cccaactccg attccgccct ctaatgtggc taccgaactt
    10621 ttatcgttta attttaacga gacatcagga caatcgctat ctgaaaccaa gaccaatgcc
    10681 agttttacca tcaacggacc ttctggttcc gctgaaagaa ttggtggtgt tgaaagcaat
    10741 gcattaagag ttaatggttt ttatggatgg gctaccggaa atgcaagcgc aacttatccc
    10801 acctcaaaag tatgtgtctc tggctggatt gcaccatccg cttttcctgt tcaaagaaaa
    10861 gatttagacc ccattaccga aaatacaaac gccgctattt tttcaaatgt caatacatcc
    10921 actagcgctg ga
//
LOCUS       DAOSNN010000060        10861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6034_length_10861_cov_4.824912, whole genome shotgun sequence.
ACCESSION   DAOSNN010000060 DAOSNN010000000
VERSION     DAOSNN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10861
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6034_length_10861_cov_4.824912"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /gene="vorB"
                     /locus_tag="PLQ70_04620"
     CDS             <1..910
                     /gene="vorB"
                     /locus_tag="PLQ70_04620"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HQE33933.1"
                     /translation="GKKAMTSSSSPGISLKLEGISYLAGAELPAVIVNVVRGGPGLGT
                     IQPSQADYFQSVKGGGHGDYKLYVLAPASVQEMADFVEDAFDIAFKYRAPVLILSDGL
                     IGQMMEKVILKDQKPRLTDAELIEKQGNWAVTGKKGRERNIITSLDLQSERMEARVHR
                     LQKKYAQMEQENLRFEKINCDDADYLIVAYGSSARISQKAMELARAKGIKVGLLRPIT
                     LFPFPKQELFELANQVKGILSVELNAGQMVEDVRLSVEHKIPVEHFGRTGGIIHTPEE
                     IVAALEQKIINNGSFRHHKTRESSIL"
     gene            864..1640
                     /locus_tag="PLQ70_04625"
     CDS             864..1640
                     /locus_tag="PLQ70_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQE33934.1"
                     /translation="MEVLDIIKPENQVFCKSQLMTDTPLSYCPGCGHGTANNLIMEVI
                     DEMGISEDTIGVAPVGCSVLAYDFMNIDMTQAAHGRAPAVATAIVRTWPEKYVFTYQG
                     DGDLAAIGTAETIHACNRGENMVIFFINNGIYGMTGGQMAPTTLEGMKSSTSPYGREI
                     ATMGSPLKITELIANLPGVYSVSRHALHTHKHVRKAKIAIRQAFENTRLKKGLSFIEI
                     LSNCNSGWKMTPNDANEWMVENMFPYFPLGDIKVEGKLKV"
     gene            1644..2207
                     /locus_tag="PLQ70_04630"
     CDS             1644..2207
                     /locus_tag="PLQ70_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQE33935.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGIMQDQEVSWLPSYGPEMR
                     GGTANVTVILSDERISSPYLKIVDTAIILNQQSMDKFEASVKPGGILIYDPNGITAHP
                     TRTDIKIYQIEGTKLAAEMGNKKIFNMVILGGYLKIKPVVKIENVIEGLKKSISERYH
                     HLIPLNLEAITLGMNSIVPYKVVEPVS"
     gene            2475..3572
                     /locus_tag="PLQ70_04635"
     CDS             2475..3572
                     /locus_tag="PLQ70_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQE33936.1"
                     /translation="MIFKKCSFLFLSLSFFIASSFQNLQAQKNFFSSAGRVEILDQNK
                     FILIGAASSVSFDFKGNSCSISLQSLVDHQNYVSIELDGKYVGRIRIEKGEMKSFPIE
                     VSGKKKTHQISIYKATEAANGGVLFAGTTAQLIESPAPKIKKKIELIGDSITCGYGND
                     DSAIACGAGDWFDQHNAYWAYGPVLSRALDLDYVLSSVSGYGMYRNWNDEHLLEPIIP
                     DVYENLYLNKDSSKPYDFKFQPDLVSICLGTNDLSEGDGKKTRLPFNEEKFISNYIEF
                     VKTVYKHAPNTRVVLLNSPMVSGERNVTFINCLKKVIQAFENDNAHKKIELFEFQPMQ
                     PKGCGYHPNIADDKVMADQLIPYFKKLLDEK"
     gene            3562..4941
                     /locus_tag="PLQ70_04640"
     CDS             3562..4941
                     /locus_tag="PLQ70_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQE33937.1"
                     /translation="MKNNLKAFVFFLLLSNTAFANVTLPNVISDNMVLQRNSDVKIWG
                     WANPQEEVVIAPSWNNQTYKIKASNQAKWEITIPTPKEGGPYTISIKGYNEIVLKNIL
                     IGEVWVCSGQSNMEMNASWGIENGDEAVKAATNPSIHFFVVPKLTATTPQNNLSGNWT
                     ECTPETMKYFSATGYFFAKRLQEELKNVPIGLISSNWGGTPAEIWTPEEVIENNEVLL
                     ESAKTRREESYGPNQPGRAFNAMIYPLTNFKIAGVIWYQGESNVGSPVYDKTFSALIT
                     SWRKLWNTDFPFYYVQIAPYKYGENHFGGAVIRDAQRKVLQEVPNTGMAITSDISPID
                     DIHPKDKKSVGTRLANLALFNTYKTNTNLVNGPLYKAIQIDKNKVIVSFDFAEGLHFT
                     HTKTNQFELAGIDGVFYEAIAVIKNNTVILQSDKVKNPVKVRFAWKNTDQSQLFNKAN
                     LPASSFLSE"
     gene            4977..7271
                     /gene="bglX"
                     /locus_tag="PLQ70_04645"
     CDS             4977..7271
                     /gene="bglX"
                     /locus_tag="PLQ70_04645"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HQE33938.1"
                     /translation="MIHKNLIFAGTFCLLAFGTLNAQKKTYLDKSKPTEERIDLLMKQ
                     MTIEEKVGQMNQYNGFWEVTGPAPKGGNAEVKYEHLRKGWVGSMLNVRGVKEVRAVQK
                     IAVEETRLGIPLIIGFDVIHGYKTLSPIPLAESASWDMEAIKKSAQIAADEASAVGIN
                     WTFAPMIDIYRDARWGRVMEGAGEDPYLGSKIAIARVKGFQGETKADLLKVNTIAACA
                     KHFAAYGFAEAGRDYNTVDISNSTLYNTVLPPFKAATDAGIRTFMNSFNILNGVPATG
                     SSFLQRDILKGNWKFDGFVVTDWGSIREMIVHGYAKDGAEASERAVNAGSDMDMESSL
                     YVAELVKLVQEGKVKETLIDDAVRRILRVKFELGLFDDPYKYCDEAREKSIVGSKANN
                     DGVLDMAKKSIVLLKNDKNLLPLKKSGQKIALIGALANDKNSPLGSWRIAADDNTAVS
                     VLEGMQQYKGNQLTFEKGADLTIGKTSFTSEVVFNTTDKSGFEAAKKVAANADVVVMV
                     LGEIGFQTGEGRSRTNLDLPGLQQELLEEIYKVNPNIVLVLNNGRPLTLPWAAKNIPT
                     IVEAWQLGTQTGNAVAQVLYGDYNPSGKLPMSFPRNVGQCPIYYNNYSTGRPGNSDTN
                     VFWSHYTDVEKTPQFPFGFGLSYTTFDYKNLKINKTAFAKGEPVTVSVEVTNSGNYDG
                     KEVVQLYIHDEFGSLARPVKELKGFELVELKKGETKTVSFILTDKELGFYDNEGNYLV
                     EPGTFKIMVGGSSDKGLESGFELK"
     gene            7435..8094
                     /locus_tag="PLQ70_04650"
     CDS             7435..8094
                     /locus_tag="PLQ70_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33939.1"
                     /translation="MIQKFINRTNTTEFYQDSDLKLQSYIYNPSNNTLEIFLSINQIS
                     YDIPVEYEEWKLTCRNTESFNGFFYELMLPYVKLKILENHPLLLKYHENELECEIKGK
                     PENVNEFIGEISNVLEKETGNWITVNEYFWNSEERFKKYSHRIISIPKSLEKSIKQVC
                     EKHKLSFNVNNETIGDDKGYADKPKAKVLIFGNEDVCPNDFFLNQSYIIAEEFIAERI
                     K"
     gene            complement(8178..10166)
                     /locus_tag="PLQ70_04655"
     CDS             complement(8178..10166)
                     /locus_tag="PLQ70_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HQE33940.1"
                     /translation="MTKTIFYFLALIFINLSFGQKKQDFVLTSPNGKIQVTIAITDII
                     TWTILHQKEVVLTPSTMSLTLDKNEVLGQNSVLLNSKREKVNTSFESPFYKKSAIKNQ
                     YNLLLLNFKNDFSIEYRAFDDGVAYRFITKKKKDITVKSEEVSLNFDQDYSTLMPYVR
                     DLRNPKDPYISSFESHYENKKISEFAKDTLAFLPFLIDYKNHKKAVFLEANLEDYPGL
                     FVTHNKTKTGFEARFSNYPLQEKNGGFNFLNKLITQRANYLVVTKGSRNFPWRIMVIS
                     ENDKDLANNDMVQKLSEPSKIKDISWIKPGKVAWDWWNDWNIYNVSFQAGINTETYKY
                     YIDFASKNKVEYIVLDEGWSVETNIMEHNPKVDLNALIAYGKEKNVDIILWSSWMALH
                     ENTKAIFKNYANLGVKGFKVDFLDRDDAKMVNSVYEIAQKAADYKLLLDFHGMYKPTG
                     IQRTFPNILNFEGVKGMENNKWTPNDDVPTYDCSIPFIRMMAGPMDYTPGAMRNATKS
                     EFKPSHSNPVSQGTRCHQLALYTIFEAPLQMMADSPTAYMKEQESTDFIAKIPTVFDE
                     TVALYGEVGKYIAIARKKESKWYLGAITNWNARDITIDFSFLEKGKQFEAEIFSDGLN
                     ADKAAQDYKKEIVTVNSSTKLTYHLASGGGLSGIITAK"
     gene            complement(10166..>10861)
                     /locus_tag="PLQ70_04660"
     CDS             complement(10166..>10861)
                     /locus_tag="PLQ70_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HQE33941.1"
                     /translation="TKRKYTEDNTIMAWEVGNEPRLFTVENEAKFTTWLNNIVNLMDS
                     LDKNHLITTGSEGLNSSNDKIEIFERTHQNPNIDYLTMHIWPKNWNWFKADDAGKTIP
                     TTLENAGKYIDQHVKVANNLKRPIIIEEFGLPRENESLISGTPVANRDVFYNYIFNRV
                     AESVAKNGTLQAANFWGFGGEGKAVTADGKWKPGDPLTTDPPQEPQGLNSVFSSDKST
                     LEIVKKYNLKFKK"
BASE COUNT         3610 a         1807 c         2086 g         3358 t
ORIGIN      
        1 cggcaaaaaa gcgatgactt catcttcaag tcctggaatt tctctgaaat tagaaggaat
       61 atcgtatttg gctggagccg aattaccagc tgtaattgta aatgtcgttc gtggtggccc
      121 tggtttagga accatccaac cttcgcaagc cgattatttt caatcggtaa aaggtggagg
      181 tcacggcgat tacaaattgt atgttttagc accagcatcg gttcaggaaa tggccgattt
      241 tgtagaagat gcttttgata tcgcctttaa atatagagct ccagttttaa ttctttccga
      301 tggattaatt ggtcaaatga tggaaaaagt aattttaaaa gaccaaaaac caagattaac
      361 tgatgccgaa ttaatcgaaa aacaaggcaa ttgggccgtt acaggcaaga aaggacgtga
      421 acgcaatatt atcacttcac tcgatttgca atccgagaga atggaagcca gagtacatcg
      481 attgcaaaag aaatatgccc aaatggaaca agagaatttg cgtttcgaaa aaataaattg
      541 tgatgatgcc gattatctga ttgtggctta tggttcaagt gccagaatat cgcaaaaagc
      601 aatggaattg gctagagcca aaggaataaa agtgggactt ttaagaccca tcaccttatt
      661 tccttttcct aaacaagaac tattcgaatt agccaatcaa gtcaaaggaa ttttaagtgt
      721 ggaattaaat gcaggacaaa tggtagaaga tgtgcgactt tcggtagaac ataaaatacc
      781 ggtagaacac tttggaagaa ctggagggat tattcacact ccagaagaaa ttgtagctgc
      841 tttagaacaa aaaatcatca acaatggaag ttttagacat cataaaacca gagaatcaag
      901 tattttgtaa atcgcaatta atgaccgata cgcctttgtc gtattgtccg ggttgtggtc
      961 acggaacggc caataatttg attatggaag tcattgatga aatgggaatt tctgaagata
     1021 ctattggggt tgcgcctgta ggttgttccg ttttagccta tgattttatg aacatcgata
     1081 tgacccaagc cgctcacgga cgtgctccgg cggtggcaac agccattgtg agaacttggc
     1141 ccgaaaaata tgttttcacc tatcaaggag atggtgattt agccgccatt ggcactgccg
     1201 aaacgattca cgcttgcaac cgaggcgaaa atatggttat cttttttatc aataatggaa
     1261 tttatggaat gactggtgga caaatggcac caacaactct tgaaggaatg aaatcctcaa
     1321 cttcgcctta cggaagagaa attgctacga tgggttctcc cttaaaaata accgaattaa
     1381 ttgctaacct tcctggagta tattccgtga gtcgtcacgc acttcatact cataaacatg
     1441 ttcgaaaagc taaaatagcc attaggcagg cctttgaaaa tacccggttg aaaaaaggac
     1501 tttcgtttat tgaaatccta tccaactgca attcgggttg gaaaatgact cccaacgatg
     1561 ccaacgaatg gatggttgaa aatatgtttc cctactttcc tcttggggat attaaagtcg
     1621 aaggaaaatt aaaagtataa atcatgactg aagaaataat tatagcaggt tttggtgggc
     1681 aaggagtact ctcgatggga aaaatattgg cctattctgg aataatgcaa gatcaggaag
     1741 tgagttggtt gccgtcttat ggtccagaaa tgcgaggagg aacggcaaat gttactgtga
     1801 ttttgagtga cgaaagaatt agttcgcctt atttaaaaat agtagatacc gccattattt
     1861 tgaaccaaca atcgatggat aagtttgagg catctgtaaa accgggaggc attttaattt
     1921 atgaccctaa cggaatcacc gcacatccca caagaaccga cattaaaatt taccaaatcg
     1981 aaggcaccaa attagcggct gaaatgggca ataaaaaaat cttcaatatg gtgattttag
     2041 gtgggtattt gaaaataaaa cccgtagtaa aaatagaaaa tgtgattgaa ggattaaaaa
     2101 aatcaatctc cgagagatac catcatttaa tccctttaaa cttagaagca ataacattgg
     2161 gaatgaatag tatcgttcct tacaaagttg ttgaacccgt ttcttagaac gaaaaaaata
     2221 gtaaaaatca ttttgaatta gtaacccatt gattttttat atatgacctt tgaagtacaa
     2281 ttgcttcaaa ggtcttattt ttttgataat ataatacctt tataattcaa tttatcatcc
     2341 aatgggtttg gcataaaaat cgagccgagt atatagaaat ttgcatattt tagattataa
     2401 attgacagtt ttgtatcttt atttttggta tttatttaat atttttactg ctttcaacat
     2461 aaattcttaa aaaaatgatt ttcaaaaaat gctcttttct ttttctgtcc ttgtcttttt
     2521 tcatagcttc ttcgtttcag aatttgcaag ctcaaaaaaa ctttttttct tcagcaggaa
     2581 gggttgaaat tcttgaccaa aacaagttca ttctcatcgg agcagcttcg tctgtgtctt
     2641 tcgatttcaa aggaaattcc tgttctattt ccttgcaaag tttagtagat catcaaaatt
     2701 atgtttccat agaactggat ggtaaatatg tgggaagaat tcgaattgaa aaaggagaaa
     2761 tgaaatcctt tccgattgaa gtttcaggaa agaaaaaaac gcatcaaatt tctatttata
     2821 aagctacaga agcagctaat ggaggcgttt tatttgcagg cacaactgcc caattaatag
     2881 agagccctgc tccaaaaatt aagaaaaaaa ttgaactcat tggcgattcc ataacttgtg
     2941 gctatggcaa tgatgattct gcaattgctt gtggagcagg ggattggttt gatcaacaca
     3001 atgcgtattg ggcttatgga ccagtgcttt ccagagcttt ggatttggat tatgtgctga
     3061 gttcggtttc gggttacgga atgtatcgca attggaatga tgagcatctg ttagaaccca
     3121 ttattcccga tgtatatgaa aatttatacc tcaataaaga cagttcaaaa ccctatgatt
     3181 tcaaatttca acccgatttg gtaagcattt gtttaggaac caacgacctt tccgaaggcg
     3241 atggcaaaaa aacgagattg cctttcaatg aagaaaaatt tatttcgaat tatattgaat
     3301 ttgtcaaaac ggtttacaaa cacgctccca atacccgagt tgttttgctc aatagtccaa
     3361 tggtttcggg cgaaagaaat gtaactttta tcaattgttt gaaaaaagta attcaggcat
     3421 tcgaaaacga caatgctcac aaaaaaattg aattattcga attccaaccc atgcagccaa
     3481 aagggtgtgg ctatcatcct aatattgccg atgacaaagt gatggccgac caattaattc
     3541 cttactttaa aaaattactc gatgaaaaat aatttaaaag cttttgtttt tttcctacta
     3601 ctttcgaata cggctttcgc caatgtgacg cttccgaatg tgatttcgga caatatggtt
     3661 ttgcaacgca acagcgatgt taaaatttgg ggttgggcaa atccgcagga agaagtcgtg
     3721 attgcaccaa gttggaacaa tcaaacctac aaaatcaaag ccagcaatca ggcaaaatgg
     3781 gaaattacaa ttccaactcc aaaagaaggt ggcccttata ccatttcgat aaaaggctat
     3841 aacgaaattg tcttgaaaaa cattttgatt ggcgaagttt gggtttgttc tggacaatct
     3901 aatatggaaa tgaacgccag ttggggaatt gaaaatggcg acgaagcagt aaaagctgcc
     3961 accaatccta gtattcattt ttttgtggtt ccgaaattga ccgctacaac tcctcaaaat
     4021 aatctgtcgg gaaattggac agaatgcacg ccagaaacga tgaaatattt cagtgctacg
     4081 ggttattttt ttgccaaacg tttgcaggaa gaattgaaaa atgtgcctat tggtttaatt
     4141 tcttccaatt ggggcggaac tccagccgag atttggacac ccgaagaagt gattgaaaac
     4201 aatgaagttt tacttgaatc agccaaaaca agaagagaag aaagttatgg tcccaaccaa
     4261 ccaggacgtg ccttcaatgc tatgatttat cctttgacga atttcaaaat cgcaggtgta
     4321 atttggtatc aaggcgaaag caatgtgggt tcgccggtgt atgacaaaac attttcggct
     4381 ttaataacct cttggcgtaa attatggaat accgattttc cgttttatta tgttcagatt
     4441 gcgccttata aatatggtga aaatcatttt ggaggtgccg taattcgcga tgcacaaaga
     4501 aaagtcttgc aagaagtccc gaatacagga atggcaataa caagcgatat ttctccaata
     4561 gatgatattc atccgaaaga caagaaatcg gttggaactc gtttggcgaa tttagcatta
     4621 ttcaatactt ataaaaccaa cactaatttg gttaatgggc cactttataa agcaattcaa
     4681 atcgataaaa acaaagttat agtttctttt gattttgccg aaggattgca ttttacccat
     4741 acaaaaacca atcaatttga actagctgga atcgacggtg ttttttatga agctattgca
     4801 gtcatcaaaa ataatacagt aattttacaa tcagataaag tgaaaaatcc tgtgaaagtc
     4861 cgttttgctt ggaaaaacac cgatcaatct caacttttta ataaagccaa tttaccagct
     4921 tcttcattcc tgtcagaata aataccaatt ttaactaaac caataaaaat accattatga
     4981 ttcataaaaa tttaattttt gcagggactt tttgtctatt agcttttgga actttaaatg
     5041 cccaaaaaaa aacatattta gacaaatcca aacctaccga agaacgaatt gatttgttga
     5101 tgaaacaaat gacaatcgag gaaaaagtgg gacaaatgaa ccaatacaac ggtttttggg
     5161 aagtaactgg gcctgcgcca aaaggaggaa atgccgaagt caaatatgaa catttgcgca
     5221 aaggctgggt aggttcaatg ctaaacgttc ggggagtaaa agaagttcgt gcagttcaga
     5281 aaattgcagt cgaagaaacc cgattaggaa ttcctttaat cataggtttt gatgtcattc
     5341 acggctacaa aacattaagc ccaattccgt tagccgaatc agcgagttgg gatatggaag
     5401 cgattaaaaa atctgctcag attgcagctg acgaagcttc ggcagttgga attaattgga
     5461 cttttgctcc aatgattgat atttacagag atgctcgctg gggaagagtg atggaggggg
     5521 ctggagaaga cccttatcta ggtagcaaaa tagcaatagc aagagtaaaa ggttttcaag
     5581 gcgaaaccaa agccgatttg ttaaaagtga atacgattgc ggcttgtgcc aaacattttg
     5641 cagcctacgg ttttgccgaa gcaggaagag attataatac ggttgatata agtaattcaa
     5701 cgctttacaa taccgttttg cctcctttca aagcagcgac cgatgcagga attcgcacct
     5761 ttatgaattc gtttaatatt ttgaatggag ttccagccac aggaagtagc tttttgcaaa
     5821 gagacattct aaaaggaaat tggaaattcg acggatttgt agtaaccgat tggggttcta
     5881 tcagagaaat gattgtacac ggttatgcca aagatggagc agaagcttct gaaagagctg
     5941 taaatgcagg ttccgatatg gatatggaat cttctttgta tgttgctgaa ttggttaaat
     6001 tggttcaaga aggaaaagta aaagaaactt tgattgacga cgccgttcgt agaattcttc
     6061 gtgtaaaatt tgaattagga ttatttgacg atccttataa atattgtgac gaagccagag
     6121 aaaaatcaat tgttggaagc aaagctaata atgatggtgt tttggatatg gctaaaaaat
     6181 ctatcgtttt gttgaaaaac gataaaaatt tacttccgct aaaaaaatca ggacagaaaa
     6241 tcgctttaat aggagctttg gcaaacgaca aaaatagccc attgggaagt tggagaattg
     6301 cagctgatga caacacggct gtttctgttt tggaaggaat gcaacaatac aaaggcaatc
     6361 aattaacctt cgaaaaggga gctgatttaa ccatcggaaa aacatcgttt acaagcgaag
     6421 tagttttcaa taccaccgac aaaagtggtt ttgaagccgc caaaaaagtg gctgccaacg
     6481 ccgatgttgt tgtaatggtt ttaggtgaaa ttggctttca aacaggagag ggtagaagta
     6541 gaaccaattt ggatttaccg ggattgcaac aagaattatt agaagaaatc tacaaagtaa
     6601 atccgaatat tgttttggta ttgaacaacg gaagaccact tacattgcct tgggctgcaa
     6661 aaaatattcc tactattgtt gaggcttggc aactaggaac tcaaaccgga aatgccgttg
     6721 cacaagtttt atatggcgat tataacccaa gtggaaaatt gccaatgtct ttccctagaa
     6781 acgtggggca atgtccaatt tattataaca attatagtac tggaagacct ggaaattcgg
     6841 atacaaatgt tttttggtcg cattatactg atgtggaaaa aacaccacaa tttcctttcg
     6901 gatttggatt gagttatacg actttcgatt ataaaaattt gaaaatcaac aaaacggctt
     6961 tcgccaaagg agaacctgta actgtttcgg ttgaagtgac taattcagga aattacgatg
     7021 gtaaagaagt agttcaattg tacattcacg atgagtttgg aagtttggca agaccagtaa
     7081 aagaactaaa aggttttgaa ttggtcgaat tgaaaaaagg tgaaaccaaa acggtaagtt
     7141 ttattttgac agacaaagag ttgggcttct atgataatga aggcaattat ttggttgaac
     7201 cgggaacttt caaaattatg gtaggaggaa gctccgacaa aggtttggag agtggttttg
     7261 aattgaaata gagaaataga cactaatatt ccactaatta ccacgaatta atttgtggaa
     7321 attagtggaa tttttgtttt aaaattagtt gtatatttgg aatattacag gtaggtatga
     7381 gttgtatgca tttatacaac gaaccaaaga aaaacactgt ggaaactaaa aaaaatgatt
     7441 caaaaattta ttaatagaac taatacaaca gaattttatc aggattctga tttaaaactt
     7501 caatcgtaca tttataaccc ttcaaataat actttagaaa tatttttatc aattaatcaa
     7561 attagttatg acattcctgt agaatatgaa gaatggaaat taacttgtcg aaatacagaa
     7621 agttttaatg gcttttttta tgaacttatg ttgccatacg taaagttaaa aattctagaa
     7681 aatcatcctt tgctattaaa ataccacgag aatgaacttg agtgcgaaat aaaaggaaaa
     7741 ccagaaaatg ttaatgaatt tattggtgaa attagtaatg ttttggagaa agaaacagga
     7801 aattggatta ctgtaaatga atatttttgg aatagtgaag aacgattcaa gaagtattca
     7861 catagaataa ttagcattcc aaaatcttta gaaaaatcta taaaacaagt ttgcgaaaaa
     7921 cataaattaa gttttaatgt aaataatgaa acaattggag atgataaagg ttatgcagac
     7981 aaaccaaagg ctaaagttct aatatttggt aatgaagatg tatgtccaaa tgactttttc
     8041 ttaaaccaaa gttacataat tgcagaagag ttcatagctg aaagaatcaa ataaaaataa
     8101 acgcatttca caaccattta tgacaattaa acctgattta aggaagtaag tttaaatatc
     8161 taaattcact ccttttttta tttagcagta attattccag ataacccacc accgctagcc
     8221 aaatgataag tcaatttagt tgaagaattt acagtaacaa tttctttttt ataatcttgt
     8281 gcagctttgt cagcatttaa gccgtcagag aaaatttcgg cttcgaattg ttttcctttt
     8341 tctaagaacg aaaaatcaat tgtaatatct ctagcgttcc agttggttat ggcacccaaa
     8401 taccatttag actctttttt tcgagctatc gcaatatact ttccaacttc gccgtacaaa
     8461 gccactgtct catcaaaaac ggttggaatt ttggcaataa aatcagtcga ttcttgctct
     8521 ttcatataag ccgtcggact gtcggccatc atttgcaaag gagcttcaaa aatggtatat
     8581 agcgccagtt gatggcatct cgttccttga cttactggat tggaatggct gggtttgaat
     8641 tcgctttttg tagcatttcg catcgctcct ggagtataat ccattggtcc cgccatcatt
     8701 cggataaacg gaatcgagca atcataagta ggaacatcat cgttgggagt ccatttgttg
     8761 ttttccattc ctttaacccc ttcaaagttc aaaatattgg gaaaagtacg ttgaattcca
     8821 gttggtttgt acatcccatg aaaatcgagt aacaatttat aatcagcggc tttttgagca
     8881 atttcataga ctgaattaac cattttagca tcgtctctgt ccagaaaatc cactttaaaa
     8941 cctttgacac ctaaattagc gtagtttttg aaaatcgctt tggtattttc gtgtaaagcc
     9001 atccaagaac tccacagaat aatgtccaca tttttctctt tgccataagc gattaaagca
     9061 tttaaatcca cttttggatt gtgctccata atgttggttt ctacactcca accttcatcc
     9121 aaaacgatgt attcgacttt gtttttggaa gcaaaatcaa tataatattt gtaggtttcg
     9181 gtgttgattc cagcctgaaa actgacgttg taaatattcc aatcgttcca ccaatcccaa
     9241 gctacttttc cgggttttat ccagctaata tctttgattt ttgaaggttc agataatttt
     9301 tgaaccatat cattgttggc taaatctttg tcgttttcag aaataaccat aatccgccaa
     9361 gggaaattcc gactgccttt tgtgacaacc aaataattgg ctctttgggt aattaatttg
     9421 ttgagaaagt tgaaaccccc atttttttcc tgcaaaggat agttggaaaa tcgagcttca
     9481 aaacctgttt tggttttgtt atgggttaca aataatcctg gataatcttc caaattagct
     9541 tccaagaaaa ctgctttttt atggttttta taatcaatta aaaacggtaa aaaagccaaa
     9601 gtatctttgg caaattcgct aatttttttg ttttcgtaat gcgattcaaa cgaagaaata
     9661 taagggtctt tcgggtttct taaatcccga acataaggca ttaaagtgct gtaatcttga
     9721 tcgaaattta aactgacttc ttccgatttt acagtaatat ccttcttctt tttggtaata
     9781 aaacgatacg caacaccatc atcaaaagca cggtattcaa tgctgaaatc atttttaaaa
     9841 tttaaaagca gcagattgta ctggtttttg atggcgcttt ttttataaaa aggggattca
     9901 aatgaagtat ttactttttc tctttttgaa ttcaaaagca cactattttg tcctaaaact
     9961 tcatttttgt ctaaagttaa tgacattgtc gatggtgtca aaacaacctc tttttgatgc
    10021 aaaattgtcc aagttatgat gtcagtaatt gcaattgtaa cttgaatttt tccgtttgga
    10081 gaggttaaaa caaagtcttg ttttttttgt ccaaatgaaa gatttatgaa aattagtgct
    10141 aaaaagtaaa agattgtttt agtcattatt ttttgaattt caaattgtat ttcttcacga
    10201 tttctaaagt agatttatca gaagaaaaaa cagaattcaa accttgtggt tcttgtggtg
    10261 gatcggttgt taatggatcg ccgggtttcc attttccatc agcggtaact gcttttcctt
    10321 cgccaccaaa tccccagaaa tttgccgctt gtaatgtgcc atttttagca acactttcgg
    10381 ccactctgtt aaaaatataa ttgtaaaaaa catctcggtt ggcaacagga gtaccgctta
    10441 ttaaactttc gttttctctt ggtaaaccaa attcttcgat aataatgggt ctttttagat
    10501 tgttagcaac tttgacatgc tggtcaatat attttcctgc attttctaaa gtggttggta
    10561 ttgtttttcc ggcatcatcg gctttgaacc aattccaatt tttgggccaa atgtgcatcg
    10621 ttagataatc gatatttgga ttttgatgcg ttctttcaaa aatttcaatt ttgtcattcg
    10681 aactattcaa cccttctgaa ccagtcgtaa ttaaatgatt tttgtctaaa ctatccatca
    10741 aattgacaat attgttgagc caagtggtaa atttcgcttc gttttcgaca gtaaaaagcc
    10801 ttggttcgtt tccaacttcc caagccataa tggtgttgtc ctccgtgtat tttcttttgg
    10861 t
//
LOCUS       DAOSNN010000061        10840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6055_length_10840_cov_7.525267, whole genome shotgun sequence.
ACCESSION   DAOSNN010000061 DAOSNN010000000
VERSION     DAOSNN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6055_length_10840_cov_7.525267"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..260
                     /locus_tag="PLQ70_04665"
     tRNA            175..260
                     /locus_tag="PLQ70_04665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:209..211,aa:Leu,seq:taa)
     gene            complement(550..750)
                     /locus_tag="PLQ70_04670"
     CDS             complement(550..750)
                     /locus_tag="PLQ70_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33942.1"
                     /translation="MKKVLLSLAVVAVLTVVSCKKAEEAPAAEAVDTTAVAVDTAAVV
                     ADTAAVVADTAAAPAAEAPAAK"
     gene            complement(974..3112)
                     /locus_tag="PLQ70_04675"
     CDS             complement(974..3112)
                     /locus_tag="PLQ70_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HQE33943.1"
                     /translation="MKILKAFLFFFVIQLQAQQGGMWIPSLLQGMNEKEMKNLGMKMS
                     VKDIYDVNQSSLKDAVPHFNGGCTSEVISPKGLILTNHHCGFDAIQNHSTVEHDYLTN
                     GFWAYKMDEELPNENLVVTFIVRIEDVTTKVLEGTSNLTSEAEKQKKIQDNISALSVS
                     LPKEAWQENKIRTFFEGNQYMLFVTETFNDVRLVGAPPSSIGKFGNDTDNWVWPRHTG
                     DFSLFRIYADKNNHPASYSKDNIPYTPKHFLPISLDGVAEDDFTMVFGYPGKTNEYLP
                     AVAIAQIANELNPAKIEIRDHALKVADGFMRKDNAIKIQYAAKYASIANYWKKWIGET
                     QGLKKSNAVAIKKNQEIAFQEKVLKAGKQTEYGHLLSDFEKDYTEIAPYALARDYFTE
                     VALRNTELLSVGFKLYQLEQVYNSKGDQAFTDRKTNLIAGLADFYKDFNPTVDEKVFE
                     QLIDLYATKVPKEFLPTNLSNINATHLATEIYSQSKLVDYKSLKELLSGDSKTVLTHL
                     NEDKGFLLIKGMSDKFLKEVAPKYEEINLRITALQRTYMKAQLELNKDTRIFPDANST
                     LRVTYGKIKGYAPKDATLYTPITYLDGVMEKYIPSDYEFDVPTKLIDLYKTKNYGVYG
                     ENGKMPVCFIATNHTTGGNSGSPAIDAKGNLIGLNFDRVWEGTMSDIYYDPSICRNIM
                     VDARYILFIIDKFAGAKNIIDELKLVHPKK"
     gene            complement(3142..4377)
                     /locus_tag="PLQ70_04680"
     CDS             complement(3142..4377)
                     /locus_tag="PLQ70_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HQE33944.1"
                     /translation="MLFLYNIIVQIANFSLKIIATFSPKIKLFVDGRKPVFDILKDKI
                     KPTDKTIWFHAASLGEYEQGLPVIEKIKEKFPSHKIVVTFFSPSGYEVRKNNTVADVT
                     VYLPLDTQKNAQQFLKLVHPEMAFFIKYEYWPNYLNEVRKIKTPTYLISGIFREKQMF
                     FKWYGRFYRKALDTFTYFFVQNESSKKLLLQLGKANVAVSGDTRFDRVAAILEKDNSL
                     DFIEAFKNDTLTIVVGSSWPKDENLLVDFINQTSENVKFIIAPHNIKSEQIQELKNSI
                     SKKVVLFSEKETKNLADFDVFIIDTIGILTKIYSYADIAYVGGGFGNPGVHNILEPAT
                     FGVPIVIGSNFSHFAEATALVNMEGCISIANKNELLDAFSNLIRNDDIRNEKGHICST
                     FVQMNKGATNIILKQILND"
     gene            4534..5664
                     /locus_tag="PLQ70_04685"
     CDS             4534..5664
                     /locus_tag="PLQ70_04685"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQE33945.1"
                     /translation="MKKLQMVDLKSQYDKIKEVVNTSIQEVLDTNTYINGPQVHQFQK
                     NLEEYLDVKHVIPCANGTDALQIAMMGLDLKPGDEVITADFTFAATVEVIALLQLTPV
                     LVDVNMVNMNISIEAIKKAITPKTKAIVPVHLFGRTVNMDAIMAIAKEHNLYVIEDNA
                     QAIGANYTFADGTKKKAGTIGHVGATSFFPSKNLGCYGDGGAIFTNDDALAHKIRGIV
                     NHGMYERYHHDVVGVNSRLDSIQAAVLNAKLPLLDSYNVARQNAARKYSAAFEGHKNI
                     IAPTICEQCDCHVFHQYVLRILDADRDALMQHLQDKGIPCAIYYPIPLHSQKAYADAR
                     YKEEDFPVTNQLVKEVIALPMHTELDDEQIKFITDSVLEFLK"
     gene            5672..6112
                     /locus_tag="PLQ70_04690"
     CDS             5672..6112
                     /locus_tag="PLQ70_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQE33946.1"
                     /translation="MKYIFTIFVVVLFQFSVFGQNNSKDKTAVAARVETLRQAMIDAD
                     GAKLKELTSVGLSYGHSSGNIQNQATFIEKIVTGESDFVTIEFQNQTIEIVGDAAIVR
                     HNLVAHTKDGGLDKDIKIGIMLVWQKQKNKWLLIARQAYKLPTT"
     gene            6121..7134
                     /gene="galE"
                     /locus_tag="PLQ70_04695"
     CDS             6121..7134
                     /gene="galE"
                     /locus_tag="PLQ70_04695"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQE33947.1"
                     /translation="MKILVTGGLGFIGSHTVVELQNQGFDVVIIDDLSNSSEDVLKGI
                     TAITNKTPEFEKLDLREKAKVQEFFKKHQDISGVIHFAASKAVGESVGNPLLYYENNI
                     NALVYLLQELQQKSEASFIFSSSCTVYGQAEVMPITENASIQTAMSPYGNTKQIGEEI
                     ITDTAKVTNMNAILLRYFNPIGAHPSAEIGELPLGVPQNLVPFITQTGFGLRQELSVF
                     GGDYPTPDGTCIRDYIHVVDLAKAHVIAMQRLLEKKNLEKIEIFNLGTGTGSSVLEVI
                     HSFEKVSGQKLPYKIVPRREGDVISAYANTDKANNVLGWTAQSTLDQAIESAWKWEQK
                     VRS"
     gene            complement(7321..8193)
                     /gene="fabD"
                     /locus_tag="PLQ70_04700"
     CDS             complement(7321..8193)
                     /gene="fabD"
                     /locus_tag="PLQ70_04700"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQE33948.1"
                     /translation="MKAYVFPGQGAQFIGMGKDLYENSALAKELFEKANEILGFRITD
                     IMFEGTAEELKETKVTQPAVFLHSVILAKTLEDFKPEMVAGHSLGEFSALVANGVLSF
                     EDGLKLVSQRALAMQKACEIKPSTMAAVLALADNVVEEVCASIDGIVVAANYNCPGQL
                     VISGELSAVEKACEALKAAGAKRALILPVGGGFHSPMMEPAREELAAAIESTTFSTPI
                     CPVYQNVTATAVSDANEIKKNLIIQLTAPVKWTQSVQQMIADGATLFTEVGPGKVLTG
                     LIGKINKEAATANA"
     gene            complement(8367..8894)
                     /locus_tag="PLQ70_04705"
     CDS             complement(8367..8894)
                     /locus_tag="PLQ70_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQE33949.1"
                     /translation="MATTKWAIDPTHSEIGFKVKHMMFTNVSGKFGTYDAIITTEDDN
                     FENASFEFSADIDSISTNNTDRDNHLKSGDFFDAENHPKLTFKATSFAKDGDDYELTG
                     DLTLRGVTKSVKFPTEFSGLMKDPWGNTKAGLNILGKINRKDWGLNWNSALETGGVLV
                     GEEVKMNIELQLIKL"
     gene            complement(9053..9277)
                     /locus_tag="PLQ70_04710"
     CDS             complement(9053..9277)
                     /locus_tag="PLQ70_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HQE33950.1"
                     /translation="MDSNNLKKEYTNGEVTIVWQSGKCIHSAMCVKNNPDVFHPKEKP
                     WIVPENSTSEKIMETINKCPSGALTFYKNK"
     gene            complement(9287..9571)
                     /locus_tag="PLQ70_04715"
     CDS             complement(9287..9571)
                     /locus_tag="PLQ70_04715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE33951.1"
                     /translation="MEELIGLEINGSKGYFYVSVNGKQEGKMTFVFAGNDKIIIDHTE
                     VNPGNNGKGYGKKMVAKAVEYAREKNIKIIPLCPFAKKVFDKTPEFRDVL"
     gene            complement(9582..10298)
                     /locus_tag="PLQ70_04720"
     CDS             complement(9582..10298)
                     /locus_tag="PLQ70_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQE33952.1"
                     /translation="MKTTVLHKADTRGHADHGWLNAYHSFSFASWYNPDRIQFGMLRV
                     LNDDTVAAGMGFGTHPHDNMEIITIPLEGDLAHKDSMGNAATIKTGDVQVMSAGTGIQ
                     HSEFNPNADQHTKLFQIWVFPKYRNVEPRYQQITLDTTEQKNNFAQILSPNPDDAGVW
                     IHQDAWFHLADFEAGFAKTYELKKEGNGLYVFVISGTINVDGQKLETRDGLGITDFKT
                     LDFKATTDAKFLLMEIPMKQ"
     gene            complement(10378..>10840)
                     /locus_tag="PLQ70_04725"
     CDS             complement(10378..>10840)
                     /locus_tag="PLQ70_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQE33953.1"
                     /translation="NAGEICKNSYFVNSGVLRSFNINDNIVEHVLSFACSGWWISDMY
                     SLLSQKPGNLFIQVIEDAEIVVLSKENQEILYQEIPKLERFFRILIENSLVAYQERLM
                     DNLSLTAEERFEKFCEKHHDLIQKVPQKQIASYLGVTPEFFSKMKSKMLRK"
BASE COUNT         3325 a         1909 c         1983 g         3623 t
ORIGIN      
        1 agagcgcgac cttgccaagg tcgaggtcgc gggttcgagc cccgtttttc gctcataacc
       61 atacgcttag taaatttaat tatttttaat tagattaaaa agtaaaatgc tcggatggtg
      121 ttgtatttaa ttaatttttt ctaaaaatta gaatataaca tttaaaggca aaatgctcgg
      181 atggtgaaat tggtagacac gctggactta aaatccagtg aacagcaatg ttcgtgcggg
      241 ttcaagtccc gctctgagta caaagaaagc cgtaaagaat taatttttaa ttctttgcgg
      301 ttttttttat ttattttaaa ttgttatcat attaataaat tttattaaag ccgaatcgct
      361 tttttgatcg aattaagatt ttaaatttat caaataaaaa aaagtcatag cactattgag
      421 tgtatggctt tttttttgtt ttctacaata tgttttaaat atttatagga aatatttgaa
      481 gtttgttata aaatgcgcat aaaaaaaagc cacgcaatgc gtagcttatt tttttactgt
      541 aatttagaat tacttagcag ctggagcttc agcagctgga gcagcagcag tatcagctac
      601 aacagcagca gtatcagcta caacagcagc agtatcaaca gctacagcag tagtatctac
      661 agcttcagca gcaggagctt cttcagcttt tttacatgat acaacagtca aaacagcaac
      721 aacggctaaa cttaaaagta cttttttcat cttacttaat tataaaaggt taattattaa
      781 ttcggggcaa agatataaat tttttaatag ttaaaatatt ttttcattat ttttttaaaa
      841 aaaatatttt gctctgttca accacagttt gaattaatag tcatattgac tttaattcca
      901 ttgattaata aatatctgac aaacccaatc gactatttta aagggtgttt ttttgatgtt
      961 ttttatctga attttatttt tttggatgaa ccaatttcaa ttcatcaatt atgttttttg
     1021 ccccagcaaa tttgtcaatg atgaacagaa tatagcgagc atctaccata atatttcggc
     1081 aaatgctagg gtcataataa atgtcgctca ttgttccttc ccaaactcta tcgaaattca
     1141 gtcctatcaa attgccttta gcatcaattg ctggacttcc tgaattgcct ccagtggtgt
     1201 gatttgtggc gataaaacaa acgggcattt tgccgttttc gccatatact ccgtagtttt
     1261 tggttttata caaatcgatt aattttgttg gaacatcaaa ttcataatca cttggaatgt
     1321 atttttccat aacaccatcc aaataggtta ttggtgtata gagagtggca tcttttggag
     1381 cgtatccttt tatttttcca taggtaactc ttagagtgct attggcatct ggaaaaattc
     1441 gggtgtcttt attcaattct aattgagctt tcatataagt tcgctgtaaa gctgtaatcc
     1501 tcaaattgat ttcttcatat tttggtgcca cctctttaag aaatttatca gacatgcctt
     1561 tgattaacaa aaagccttta tcttcattta aatgagttaa aaccgttttt gaatcgcccg
     1621 aaagcaattc ttttagactt ttataatcaa ctaattttga ttgtgagtaa atttcagtag
     1681 ctaaatgggt tgcatttata ttcgataaat tggttggcaa gaattcttta ggcacttttg
     1741 tggcatataa atcaatcaat tgttcaaaga ctttttcatc aacagttgga ttaaaatctt
     1801 tataaaaatc agctaaacca gctattaaat tggtttttct gtcagtaaac gcttgatctc
     1861 ctttggaatt gtaaacttgt tctaattgat acaatttaaa accaacactc aagagttcag
     1921 tatttcttag tgctacttcc gtaaaatagt ctctcgccaa ggcataagga gcaatttcgg
     1981 tataatcctt ttcaaaatca gaaagtaaat ggccgtattc agtttgtttt ccggctttta
     2041 aaaccttttc ttggaacgca atttcttgat tttttttgat ggccacagca ttcgattttt
     2101 tcaaaccttg tgtttctcca atccatttct tccaatagtt ggcaatactg gcatattttg
     2161 cggcatattg aattttgatg gcattgtctt ttctcataaa tccatcggca actttcaatg
     2221 cgtgatctct aatctcaatt tttgcaggat tcaattcgtt ggcaatttgt gcaatcgcaa
     2281 cggcaggcaa atactcattt gtttttccgg gatagccaaa aaccatcgtg aaatcgtctt
     2341 cagcaacacc gtctaacgaa atgggtaaaa aatgtttggg agtgtaagga atgttgtctt
     2401 ttgaataact ggcaggatga ttatttttgt cggcataaat tctaaacaaa gaaaaatctc
     2461 ccgtatggcg gggccaaacc caattgtcag tgtcgtttcc aaattttccg attgacgatg
     2521 gcggagctcc aaccaatcga acatcattaa aagtctcggt tacaaatagc atatattgat
     2581 tgccttcgaa aaacgttcta attttatttt cctgccaagc ttctttgggt aatgaaacgc
     2641 ttaaagctga aatattatct tgaattttct tttgtttttc agcttcagaa gttaaatttg
     2701 aagtgccttc caaaactttt gtggtaacat cttcaattct aacgatgaaa gttactacca
     2761 agttttcgtt gggcaattct tcgtccattt tataggccca aaaaccattt gttaaataat
     2821 catgttctac agttgaatga ttttgtatgg catcaaatcc acaatgatga ttggttaaaa
     2881 ttaagccttt cggcgaaata acttcggaag tacaaccgcc attaaaatgt ggaacagcat
     2941 ctttcaagct cgattgattg acatcataaa tgtcttttac ggacattttc atccctaaat
     3001 ttttcatttc tttttcattc attccttgca atagagaagg aatccacata ccgccttgtt
     3061 gcgcttgtaa ttggatgaca aagaaaaata agaatgcttt taaaattttc attgtaattg
     3121 atttggactg taatttcatt ttcaatcgtt taaaatttgt tttaaaataa tgttggttgc
     3181 tcctttgttc atctgaacga aagtgctgca aatgtgacct ttttcgtttc ggatgtcgtc
     3241 atttcgaatc aaattagaaa aagcgtcaag aagttcgttt ttattggcaa tagaaatgca
     3301 gccttccata ttgaccaatg ctgtggcttc ggcaaaatga gaaaaattag aaccaatcac
     3361 aattggaact ccaaaagtgg ctggttccaa aatattatga acgcctggat tgccaaaacc
     3421 gccacccaca taagcaatgt cggcatagct gtaaattttg gtcaaaattc cgatggtgtc
     3481 gatgatgaat acatcaaaat cggctagatt tttagtttct ttttcagaaa acaataccac
     3541 ttttttcgaa atggaatttt tcaattcttg aatttgctcg cttttaatat tgtgtggtgc
     3601 aatgataaac ttaacgtttt cggaagtttg attgatgaaa tcaaccaata aattttcatc
     3661 ttttggccaa gaacttccaa ctacaattgt taaggtatcg tttttgaagg cttcaataaa
     3721 atctagagaa ttgtcttttt ctaaaattgc ggccactcga tcaaaacggg tgtcaccaga
     3781 aacggctaca ttggctttgc ctaattgcaa caacagtttt ttagaacttt cattttgtac
     3841 aaaaaagtaa gtgaaggtgt ctaatgcttt tctgtaaaat ctgccatacc atttgaagaa
     3901 catttgcttt tctctaaaaa ttccagaaat caaataagta ggggttttta tttttcgaac
     3961 ttcgttcaaa taattcggcc aatattcata tttgatgaaa aaagccattt caggatgaac
     4021 gagtttaaga aattgttgcg catttttttg ggtgtccaaa ggtaaataca cggtaacatc
     4081 ggcaaccgta ttgtttttcc gaacctcata acccgaaggc gagaaaaaag taaccacgat
     4141 tttatgactg gggaattttt cttttatttt ttcaattact ggcaaacctt gttcatattc
     4201 gccaagagaa gcagcgtgaa accaaatcgt tttatcggtt ggtttgattt tgtcttttaa
     4261 aatgtcaaaa acaggcttgc ggccatcgac aaaaagtttg atttttggac tgaatgtagc
     4321 gatgattttt aatgaaaaat tcgcaatttg aacaataatg ttgtagagaa atagcatatt
     4381 gaaaaatttc tgtggcaaaa atacgttttc ttttaaatat ctataaaatc attctcctaa
     4441 aatccctatt tttgttctga attttaaaca agtcaagttt aatgaaatct tggtaaaact
     4501 ttgtttggaa tgaactttaa acaaaagatt aaaatgaaaa aattgcaaat ggttgactta
     4561 aaaagtcaat acgataaaat taaagaggtt gtaaatactt caatccaaga agttctcgac
     4621 accaacactt acataaacgg accacaagtt catcaatttc aaaaaaatct cgaagaatat
     4681 ctcgacgtaa aacatgtaat tccttgcgct aacggaactg atgctttgca aattgccatg
     4741 atggggctgg atttaaaacc cggagatgag gtaattactg ctgattttac attcgctgca
     4801 actgtagaag taattgcttt gttgcaatta accccagttt tggtcgatgt gaatatggtg
     4861 aatatgaaca tttctatcga ggctattaaa aaagcgatta caccaaaaac caaagccatt
     4921 gtgccagtac atttatttgg tcgtactgtt aatatggatg cgataatggc gattgctaaa
     4981 gaacacaatc tgtatgttat tgaagataat gcacaagcca ttggagccaa ttatactttt
     5041 gctgacggaa caaaaaagaa agcaggaacg attggccacg ttggggcgac ttcgtttttc
     5101 ccatctaaaa acttaggttg ttatggcgat ggtggagcta tttttactaa tgatgatgcc
     5161 ttggctcaca aaattagagg aattgtaaac cacggaatgt acgaacgcta ccatcacgat
     5221 gtggtgggag ttaattcgcg tttagacagt attcaagctg cggttttgaa tgctaaattg
     5281 ccacttcttg actcatataa tgtagcacgt caaaatgcag ctagaaaata ttcagcggct
     5341 tttgaagggc ataaaaatat tattgcaccc accatttgtg agcaatgtga ttgccacgtt
     5401 tttcatcaat atgtgttgcg aattttagat gcagatcgtg atgctttgat gcaacatttg
     5461 caagacaaag gaattccttg tgccatttat tacccaattc cgttgcattc gcaaaaagcg
     5521 tatgcggatg ctcgttacaa agaagaagat tttccagtga cgaatcaatt ggtaaaagaa
     5581 gtgatagctt tgccaatgca caccgaatta gacgatgaac aaattaaatt tattacggat
     5641 agcgttttag aatttttaaa ataaatttag gatgaaatat attttcacaa tttttgtggt
     5701 agttttattc cagttttctg tttttggaca gaataattct aaagacaaaa cagcagttgc
     5761 cgctcgtgtg gaaactttgc gtcaagcaat gattgatgcc gatggagcca aattgaaaga
     5821 gttgacttct gttggtttga gttacgggca ttccagtggg aatatccaga atcaagctac
     5881 ttttatagag aaaatcgtta ctggagaatc ggattttgtg accattgagt ttcaaaatca
     5941 aactattgaa attgtgggag atgctgcgat tgttcgtcac aatttagtag cgcacaccaa
     6001 agatggcgga ttagataaag atataaaaat tggtataatg ctagtttggc aaaaacaaaa
     6061 aaacaaatgg ttgctcattg ccagacaagc atataaatta cctacaacat aaaaataaat
     6121 atgaaaatac tagtaacagg aggattgggt tttatcggtt cgcataccgt agttgaattg
     6181 caaaatcaag gttttgatgt agtgattatt gatgatttat ccaattcatc agaagatgtt
     6241 ttaaaaggaa ttactgccat cacgaacaaa acaccagaat tcgaaaagtt ggatttgcgt
     6301 gagaaagcaa aagttcagga atttttcaaa aagcatcaag atatttcggg agtgattcat
     6361 tttgcagctt cgaaagcagt gggcgaaagc gttggaaatc cgttgttata ttatgaaaat
     6421 aatattaatg cgttggttta tttgctgcaa gaattgcaac aaaaatcaga ggcaagcttt
     6481 atttttagtt cctcttgcac ggtttatggt caagccgaag tgatgcccat tactgaaaac
     6541 gcatccattc aaaccgcaat gtctccttac ggaaacacca aacaaattgg cgaagaaatc
     6601 ataactgata cagccaaagt aactaatatg aatgccattt tgttgcgcta tttcaacccg
     6661 attggtgcgc atccatcagc agaaattggc gaattgcctt tgggagtgcc gcagaattta
     6721 gtgcctttta tcacacaaac tggatttggt ttgcgacaag aattatccgt ttttggaggt
     6781 gattatccta cgccagacgg aacttgtatt cgcgattata ttcacgtagt cgatttggcg
     6841 aaagcccacg tcatcgcgat gcaacgctta ttggaaaaaa agaatttgga gaaaatcgag
     6901 atttttaact tgggaacagg aaccggaagt tcggttttag aagtgattca ttctttcgaa
     6961 aaagtgagcg gacaaaaact accttataaa attgtgcctc gaagagaagg cgatgttatt
     7021 tctgcttatg ctaataccga taaagcgaac aatgttctgg gatggacagc gcaatcaaca
     7081 ttagatcaag ccatagaaag cgcctggaaa tgggaacaga aggttaggag ttagtaatca
     7141 gtgatcagtc ccgaggcttc gggattagtg ttcagtaaaa aaacgaatcc taaattatcg
     7201 agttggtaat ttaggatttt ttggtttaaa gaataatgct acaatatcag gaaaacaaaa
     7261 aatccccaca aacataagtt tatgaggatt ctgaaatctg ccttctgcaa tctgaatttt
     7321 ttaagcattt gccgttgcag cttctttatt gatttttcca attaatccag tcaaaacttt
     7381 tccaggaccc acttcggtaa acaaagttgc tccatcagca atcatttgtt gtacagactg
     7441 tgtccatttt acaggagcgg tcaattgtat gatcaagttt ttcttgattt cgtttgcatc
     7501 agaaaccgca gttgcagtta cgttttgata tactgggcaa ataggagtag agaatgtagt
     7561 agattcaatg gctgctgcca attcttctct tgcaggttcc atcattggcg agtgaaaacc
     7621 accaccaact ggcaaaatta aagctctttt ggcaccagcg gctttcaatg cttcgcaagc
     7681 tttttctact gccgataatt ctcccgaaat cactaattga cctggacagt tgtaattggc
     7741 agcaaccaca attccgtcga tagaagcgca aacttcttca acaacgttat ctgctaaagc
     7801 taaaacggca gccatagtcg aaggtttgat ttcgcaggct ttttgcattg ccaaagctct
     7861 ttgtgaaacc aatttcaatc cgtcttcaaa agacaaaaca ccattggcaa ccaaagctga
     7921 aaattcgccc aatgaatgtc ctgccaccat ttctggtttg aaatcttcca aggttttggc
     7981 caaaattacc gagtgcaaga aaacagccgg ttgggttact ttggtttctt tcaattcttc
     8041 ggcagtgcct tcaaacataa tgtcggtaat gcggaaacct aagatttcat tggctttttc
     8101 gaataattct ttggctaatg ccgagttttc gtataagtct ttacccattc ctatgaattg
     8161 tgcgccttga cccggaaata cgtatgcttt catctatttt agtttaaaag tttaaggttt
     8221 aaaagtttaa agttttctcc aagaggaaac taaaaaccgt ttgcaaaaat agtacttttt
     8281 taggatttat ttgaaaagat aaaacgaaag caaaacggct aaacttcatt gtgaaattta
     8341 gccgtttgtc tttgattaaa attgatttat agttttatca actgcaattc gatattcatt
     8401 tttacttctt cgccgactaa aacgccacca gtttctaaag ccgagttcca gttcaatccc
     8461 cagtctttac ggtttatttt gcccaaaata ttcaatcctg ctttggtgtt tccccaagga
     8521 tctttcatca atccgctgaa ttcagtaggg aattttaccg atttggttac acctctcaaa
     8581 gtcaaatcgc cagtcaattc ataatcatcg ccatctttgg caaaagaagt ggctttgaaa
     8641 gttagttttg gatggttttc tgcgtcaaaa aaatcgccgc ttttcaagtg attgtctcta
     8701 tcagtgttgt tggtgctgat agaatcgatg tcggcagaga attcgaaact tgcattttcg
     8761 aagttgtcat cttcggtagt gatgattgca tcataagtgc caaattttcc tgaaacgtta
     8821 gtaaacatca tgtgttttac tttaaaacca atttctgaat gtgttgggtc aattgcccat
     8881 tttgtagttg ccataatttt atttttaaat tgttatttca ttttgataag gcaaatttac
     8941 gacggcacta ccgatggaac acttaaccta gattaagaac gaaaaaaacc agaaaactat
     9001 ttagttttct ggttttaaat gaattcgtgt ttaaatcctt gttaggatat ttttatttgt
     9061 ttttgtaaaa cgtcaaagca cctgatgggc atttgttgat cgtttccata attttttcag
     9121 aagtggagtt ttcgggtaca atccaaggtt tttctttggg atgaaaaacg tcgggattgt
     9181 ttttaacaca cattgcagaa tgtatgcatt ttccagattg ccatacaata gtcacttcgc
     9241 cattggtata ttcttttttt aaattgttgg agtccatatt tataatttaa agtacgtccc
     9301 taaattctgg agttttatca aacacttttt tggcaaaagg acaaagcgga atgattttga
     9361 tgttcttttc tctggcatat tccaccgctt tggcgaccat ttttttgcca taacctttgc
     9421 cgttattgcc tggattaact tctgtatggt caatgataat cttgtcgttg ccagcgaaga
     9481 caaaagtcat tttgccttcc tgttttccat tgaccgaaac ataaaaatag cctttcgagc
     9541 cgtttatttc taatcctatt agttcttcca tagtattgaa tttattgttt cattggaatt
     9601 tccatcaaca aaaatttcgc atcggttgtt gctttgaagt ctaaagtttt gaaatcagta
     9661 attcctaaac catcacgagt ttccaatttt tgaccatcaa cattgattgt gcctgaaatc
     9721 acaaaaacat aaagaccgtt tccttctttt ttcaattcat aagttttagc aaatccagct
     9781 tcgaaatcag ccaaatgaaa ccaagcgtct tgatgaatcc aaactccggc atcatcagga
     9841 tttggagaca atatttgggc gaaattattt ttttgctccg tagtgtccaa agtgatttgt
     9901 tggtaacgag gttccacgtt tctgtatttt gggaaaaccc aaatctggaa aagtttggtg
     9961 tgctgatcgg catttggatt gaattcggag tgttgaattc cggttccagc actcattacc
    10021 tgtacatcgc cagtttttat agtagcggca tttcccatac tgtctttgtg ggccaaatcg
    10081 ccttccaacg gaatggtaat aatttccata ttgtcgtgtg gatgtgttcc aaaacccatt
    10141 ccggcagcca cggtatcgtc gtttaaaacg cgcaacattc cgaattgaat tctatccggg
    10201 ttgtaccagc tggcaaaact aaaactgtgg taggcgttta gccatccgtg atctgcgtgt
    10261 cctcttgtat ctgctttgtg taaaaccgtt gttttcataa tgtataaatt taatcgtttt
    10321 tggttgtaca aagatacgtt tgatgcaagc aaaagacact taatgtagat taagaaatta
    10381 ttttcgcaac attttacttt tcattttgct gaaaaattcg ggagtcacgc ccagatagga
    10441 ggcaatttgt ttttggggta ctttctgaat taaatcgtga tgcttttcac agaatttttc
    10501 gaaacgttct tcggcagtca agctcaaatt gtccatcaat cgttcttgat aggctactaa
    10561 tgaattttca ataagaatcc tgaaaaaacg ttcgagtttg ggaatttcct gatacaggat
    10621 ttcctggttt tctttggaca aaaccactat ttcggcatct tcgattactt ggatgaaaag
    10681 attgccaggt ttttgcgaaa gcaggctgta catatcgcta atccaccagc cgctgcaagc
    10741 aaaactcaat acgtgttcca caatattgtc gttgatgttg aaacttcgca acacacctga
    10801 attcacaaaa taggaattct tgcaaatttc gcccgcgttg
//
LOCUS       DAOSNN010000062        10544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6301_length_10544_cov_5.211841, whole genome shotgun sequence.
ACCESSION   DAOSNN010000062 DAOSNN010000000
VERSION     DAOSNN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6301_length_10544_cov_5.211841"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLQ70_04730"
     CDS             <1..289
                     /locus_tag="PLQ70_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HQE33954.1"
                     /translation="LGTNAPTVEQQAAQAAMYQYVIDMYKKYIPTAQQYGITIWGISD
                     NKNEHEFWLPNESPNLWDANYARKHAYKGTADGLAGKDVSLGFSGELQPKN"
     gene            complement(444..516)
                     /locus_tag="PLQ70_04735"
     tRNA            complement(444..516)
                     /locus_tag="PLQ70_04735"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(481..483),aa:Met,seq:cat)
     gene            complement(916..1698)
                     /locus_tag="PLQ70_04740"
     CDS             complement(916..1698)
                     /locus_tag="PLQ70_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HQE33955.1"
                     /translation="MKNIYTLFLVFLFSLVMKAEVSVAEKNALVQLYNATNGANWTSK
                     WDLKTPVSSWYGVKLQDDKVVAVQLQMNNLVGTLPSSIGDLKSLEVLNLSFNKLSGSI
                     PASIGELSSLKTLSMFMNSLSGNLPSEIGKLSNLETLSLYNNEIEGQIPSSLYSLKNL
                     KVLYLNSNKLSGTLNAEISNMVSLQQLSLFDNKMNGQVPFDIAKLNNLKEMNISYNQF
                     SGFVSKDLAKLDMLNMTMINNKGVAQALKVSTERNTAIVSEE"
     gene            1915..2403
                     /locus_tag="PLQ70_04745"
     CDS             1915..2403
                     /locus_tag="PLQ70_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HQE33956.1"
                     /translation="MPFNFLAILVAALSSFVVGFVWYNPKVFGTIWMKESGLTQEQCE
                     KGNMLKIFGLTFVYSLMLAFLMPTLVIHQMGTLGMIGGPDFIASAKPSYAAFLADYGD
                     AFRTFKHGALHGFMSGIFLALPIISINGLFEQKSWKYMAIQSGYWIVIMTIMGAIICG
                     WK"
     gene            2442..3608
                     /locus_tag="PLQ70_04750"
     CDS             2442..3608
                     /locus_tag="PLQ70_04750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE33957.1"
                     /translation="MGDFFYLKTPLSIAIYSSISELPSNWNNFAIDNIFLSKDYLEIL
                     QKSAPTNMICHFIGIYNEGELVGISLSQFLDLNKLESFGERDKCVKNAVRNSFLKNLC
                     SHILIIGNNMLTGQNAYAFSNKIDEVQILLALKKASQKLKVIFKNKGFKVHITTYKDF
                     PEENLTPFYQAGFKEYFQFSAQPNMHFEIPNQWKSEQDYINALSKKYRDQYKRARKKA
                     EGIEKKKMELADIIKHEETIYDLYYHVAKNAHFNTFFLTKNHFRTFKELLQDKFLFYG
                     YFLEDKLIGFNTIIKNGEVMDTYFLGYDDSIQREKMLYLNMLYDMVAYSINQGFKEII
                     FARTALEIKSSIGAKPQEMYGFAQHNNPIADLAFEKLYNYLEPKVEWKERNPFK"
     gene            complement(3611..5533)
                     /locus_tag="PLQ70_04755"
     CDS             complement(3611..5533)
                     /locus_tag="PLQ70_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQE33958.1"
                     /translation="MLNIHNLSVSFGGTYLFEEVTFRLGAGDRVGLVGKNGAGKSTML
                     KILAKDFAPDSGVISQEKEVRMGFLRQDIDFEQGRTVLEEAYEAFTDIKIVEKKLEQI
                     NHQLVTRTDYESEEYSQIIEDLSDYTHRFELLGGYNYIGDTEKILLGLGFKREVFNDQ
                     TDTFSGGWRMRIELAKLLLQANDVLLLDEPTNHLDIESIIWLESFLRNYPGVVVIVSH
                     DKMFLDNVTNRTIEISLGKAYDFNKPYSEYLELRGEIREKQLATQKNQAKKIEETEKL
                     IEKFRAKASKASMAQSLIKKLDKVERIEVDEDDNSVMNISFPVSKVPGKVVIEAENVT
                     KAYGDRVILKDISLLVERGSKIAFVGQNGQGKSTFIKAIVNEFEFQGSIKLGHNVQVG
                     YFAQNQAEYLDGEITLLQTMENAASDSNRSKVRDMLGSFLFRGDEVEKKVKVLSGGER
                     NRLALCKLLLQPINVLVMDEPTNHLDIKSKNVLKAALQKYEGTLLLVSHDRDFLQGMS
                     NIVYEFKDQKIKEYLGDINYFLEQRNLENMREVEKKDSDKLASAQSKDQKASSKASYE
                     DQKKGKALQNRLSKIESQIKQLEKDLLHDDKMLASNYDKHIEDAAFFVAYNKKKSDLD
                     QLLLDWEIVQEEIDAL"
     gene            5598..5978
                     /locus_tag="PLQ70_04760"
     CDS             5598..5978
                     /locus_tag="PLQ70_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HQE33959.1"
                     /translation="MLKKGSKLNSILTGSCPRCQNESMYSDKNPLHFSKILQMNENCS
                     HCGLQYQIEPSFFYGAMYVSYALNVALSIAVFVISYLFFNSSIKTSFIAIILANIVLF
                     PFVLRWSRNIYINLFVKFDKKYKK"
     gene            complement(5985..7028)
                     /locus_tag="PLQ70_04765"
     CDS             complement(5985..7028)
                     /locus_tag="PLQ70_04765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQE33960.1"
                     /translation="MIDYLIVGSGLAGISFSEIALQNNKSILVLDNNSQNSSEIAGGL
                     YNPVILKRFTEVSQAKNHLPIMNDFFSNLEKKLNCKVNFEMPILRKFFSIEEQNNWFF
                     ASDKVNLAPFLSTNIISRKYAGIDSPFGYGEVLQTGYVDTALLLKKYKEYLIENNWFL
                     EESFDYSLLQEEGNSIQYKEISARNIIFAEGFGMHANPYFKDLPLDGTKGELFIIKAA
                     ALDLDVIINTSVFILPLGNDLYKVGATYNWADKTDVPTEEGKTELIEKIKEILNCDFE
                     IVEHLAGVRPTVKDRRPLVGTHSKHNSIHILNGLGTRGVMLGPALAKALFENIEFQKP
                     LDREIDIQRFYKK"
     gene            complement(7109..7999)
                     /gene="gldN"
                     /locus_tag="PLQ70_04770"
     CDS             complement(7109..7999)
                     /gene="gldN"
                     /locus_tag="PLQ70_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HQE33961.1"
                     /translation="MNMRNFLIVVISIFGTVATYAQPNLLNAKIPEEIGLKSAAQLIS
                     DNDKPLEYGYVDDRDVLMGKTVWEIIDLSERINFPLYFPIDTANIGSDRRSLYDVLTK
                     AIKNGELTEVYSDSYFNTKKSYKDIQASLTRIDTTDAGREQINAGQKISPEYIVKQDL
                     TAQDVTQYKIKGYWYFDKRQSELKYRLLGICPVTPDVYTMNSEEKDYIELFWVFFPAS
                     RDVLHEAKAFNNKNSAMPVSFDQILNSRRFNAVIYQEENVYGDRAITSYMKDNAQNQL
                     LESERVKDKIRNFEEDMWNY"
     gene            complement(8045..9595)
                     /gene="gldM"
                     /locus_tag="PLQ70_04775"
     CDS             complement(8045..9595)
                     /gene="gldM"
                     /locus_tag="PLQ70_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HQE33962.1"
                     /translation="MAGGKLSPRQKMINLMYLVFIAMLALNMSKEVLSAFGILNNKII
                     ESNSITDGRNESSFQQLSQKAVDQPGQFGDKRAKVEKIRALSKEFNDYIEGIKTTVTA
                     KFERDAEGNLPYEEMDKGDLIDRMFFTGDKVSKQGKEFLDKINSYPAQIKAIGGSSIA
                     ESEMKKIEKRFATNQVYSEKAGAKLDWIDYNYKGFPLIATITKLSQLQADIKTTESDV
                     MTGMFQSDLVAAASLTAYQPIVVLEKSVFFQGEAVKGKIILGKFDPSLKAKSVVVNGS
                     SVAAQAGQANFSFGSGNIGEHPISGSFNFDENGKVISLPIKDKYVVVARPKSATISAD
                     KMNVVYRGVVNPMTISFAGISPDKVSAAAAGLTRVGNTSSYTMRPGAGTEVVINVTGT
                     LPDGSKVSDKKSFRIKGIPGPTGTIRGEMGVVKGPKSSLEISTVGAKLVDFDFEVGLD
                     VVGFNFKVTGQPTVVVVGNKLNAQCKSVLSKVGRGDQVTISEIKTKLVGAGSYLLPRT
                     APVIYEIQ"
     gene            complement(9641..10291)
                     /gene="gldL"
                     /locus_tag="PLQ70_04780"
     CDS             complement(9641..10291)
                     /gene="gldL"
                     /locus_tag="PLQ70_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HQE33963.1"
                     /translation="MALISKQKMNFAYGMGAAVVIIGALFKITHIELGFITGNLMLTI
                     GLVVEALIFALSAFEPVENELDWTLVYPELANGEARKKVAKTEVPTEDAQGMLSKKLD
                     AMLKDAKIDGELMSSLGNSIKNFESAAKAISPTVDSIASTQKYSEELTLAAAQMESLN
                     SLYKVQLQSASRNAQINEEVAENNLKLKEQMQSLTSNLSSLNSVYGGMLSAMSNKG"
     gene            complement(10332..>10544)
                     /locus_tag="PLQ70_04785"
     CDS             complement(10332..>10544)
                     /locus_tag="PLQ70_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQE33964.1"
                     /translation="EYVSSMNPNNTDTSNKRKVVRGGSWKDVAYFLQVGTRTFEYADS
                     ARSFIGFRTVQDYMGTQSTEKSKKKK"
BASE COUNT         3436 a         1943 c         1491 g         3674 t
ORIGIN      
        1 actaggcacc aatgctccta cagtagagca acaagcggct caagccgcaa tgtaccaata
       61 tgtgattgat atgtacaaaa aatacatacc tacagcccaa caatacggca taaccatttg
      121 gggaatttca gacaacaaaa acgaacacga attttggctc cctaatgaat cgccaaatct
      181 ttgggatgcc aattacgcta gaaaacacgc ctacaaagga actgccgatg gtttggctgg
      241 aaaagatgta agtttagggt tttctggaga attacaacct aaaaattagt tttaattcac
      301 attccatttt caatttggat ttaagaatgt aatttggcaa tctaaatcaa taaaaaagct
      361 gtctgaaact attttcagat agcttttttt aattccgtta atggtcataa aaaaaccatt
      421 cagaaatgaa tggtttctct tagtagcggg aacaggactc gaacctgtga ccttcgggtt
      481 atgagcccga cgagctgcct actgctctat cccgcgatgt ttcgggtgca aagatacacc
      541 gaattttaat aaatacaagt gatttatata aaaatatcta tatttaatat attacactga
      601 cttaaaagca gttaatgtta ttaaattctt attgtcttca tttaatttct aatagttcaa
      661 ttttaataat aactaggtga tgcaaagtca ttgaaaaatt accaaacgaa cctaaaatct
      721 aacttataaa acattatttt tcacaagatg aatattgaca ggtacaatgc tcttaaacgg
      781 caaaaaaaac ttattttacc ttaatccttt tgataaccat taaaaaaagg agtagaaaaa
      841 tttattttct actccttttt acactaacca atcttttttt gtaaatactt taaaaaacta
      901 actttttact tagatttatt cttctgatac aatagctgta tttctttctg ttgaaacttt
      961 caatgcttga gcaacacctt tattattgat catagtcata tttaacatat ccaacttcgc
     1021 taaatcttta gaaacaaaac cactgaactg attatatgaa atattcattt cttttaaatt
     1081 attcaactta gcaatatcaa atgggacttg tccgttcatt ttattatcaa ataaactaag
     1141 ctgttgaaga ctaaccatat ttgaaatttc agcattcaaa gtgcctgata atttattact
     1201 gtttaaatac aaaactttca aatttttcaa actataaaga ctagatggaa tttgaccttc
     1261 aatctcatta ttgtataaag acaaagtttc aagattgctt aactttccaa tttcagaagg
     1321 aagatttccc gaaagagaat tcataaacat tgacaaagtt ttcaatgaac ttaattcgcc
     1381 aatagaagct ggtatagaac ctgacaattt attaaaagat aaatttaaaa cctccaacga
     1441 tttcaaatct ccaattgaag acggcaaagt ccccactaaa ttattcattt gcaattgaac
     1501 tgcaacaact ttatcatctt gcaattttac tccataccaa gaagatactg gagttttcaa
     1561 atcccattta gaggtccaat ttgcaccatt tgtagcatta tacaattgaa caagtgcatt
     1621 tttttcagca actgaaactt ctgctttcat tactaatgaa aacaagaaaa ctaaaaataa
     1681 tgtatatata tttttcataa cgcttgattt ttgacaaatt gttaaacaaa tatacaaaca
     1741 acttagataa agttctaaaa taatcgacaa aacacacaaa tatatcttaa tttaaactat
     1801 aatcttacaa aatattgttt atacattaat tcattcttaa ttagcttcaa aaaaattacc
     1861 aaataattca aaaaaaaaat tatatttgat attctaactc aaaaacacaa aactatgcca
     1921 tttaacttct tagcaatttt agtagccgct ttgtcctctt ttgtagtagg atttgtatgg
     1981 tacaatccta aggttttcgg aaccatttgg atgaaagaat ctggtttgac ccaagagcaa
     2041 tgtgaaaaag gaaacatgtt gaaaattttc ggactcacat ttgtttattc actgatgcta
     2101 gcctttttga tgcctacttt agttattcat caaatgggta ctttaggaat gattggaggt
     2161 cctgatttta ttgcaagtgc aaaaccctcc tatgctgcct ttttagctga ttacggcgat
     2221 gcttttcgaa cttttaaaca cggtgcactt cacggtttta tgtcaggaat ttttctagcc
     2281 ttacctatta tttcaataaa tggtttattc gaacaaaaat cctggaaata tatggctata
     2341 caatctggat attggatcgt aattatgacc attatgggag ccataatttg tggctggaaa
     2401 taatttttaa catttaatta taatacaatc gctctacatt tgtgggcgat tttttttatt
     2461 tgaaaacacc tctatccata gcaatttatt cctctatttc tgaacttcct agtaattgga
     2521 ataattttgc tatagacaac atcttcctaa gcaaagacta tttagaaata ctacaaaaat
     2581 cggctccaac aaatatgatt tgtcatttta ttggaatcta taatgaaggt gaattggttg
     2641 gaatttcgct ttcacaattt ttagatttaa acaagttaga atcttttgga gaacgagata
     2701 aatgtgtaaa aaatgcagtt cgaaacagct ttttgaaaaa cttatgttcc catattctca
     2761 tcatcggcaa caatatgctt acgggacaaa acgcctatgc cttttcgaac aaaatagacg
     2821 aagttcagat actacttgcc ttaaaaaaag catcccaaaa gctaaaagta attttcaaaa
     2881 acaaaggatt taaagttcat ataactacct ataaagactt tcccgaagaa aacttaaccc
     2941 cattttatca agctggattc aaagaatact ttcagttttc agctcaacct aatatgcatt
     3001 ttgagattcc aaatcaatgg aaatctgaac aagattacat caatgctttg tctaaaaaat
     3061 accgagatca atacaaacgg gctagaaaaa aagcagaggg aatcgaaaaa aagaaaatgg
     3121 aacttgccga cattatcaaa cacgaagaaa ctatttatga tttgtattat cacgtggcta
     3181 aaaacgccca tttcaacact tttttcttaa ctaaaaatca ttttagaact tttaaagaac
     3241 tacttcagga caaattcctt ttttatggct acttcctaga agataaatta attggtttca
     3301 atactatcat caaaaacgga gaagttatgg atacctattt tttaggttat gacgatagta
     3361 ttcaacgtga aaaaatgctg tatttgaata tgctatatga tatggttgcc tactctatca
     3421 accaaggttt caaggaaatt atttttgcta gaacggcttt agaaataaaa agttcgattg
     3481 gcgcaaagcc acaagaaatg tatggatttg cccaacataa caatccaatt gccgaccttg
     3541 ctttcgaaaa attatataat tatctcgaac caaaagtgga atggaaagag cgaaatccat
     3601 tcaaataaaa ttacaaagcg tctatttctt cttgaacaat ttcccagtct aaaagcagtt
     3661 ggtctaaatc cgattttttc ttgttataag ccacaaaaaa agcagcgtct tcaatgtgtt
     3721 tgtcataatt agacgccaac attttatcgt catgcaaaag gtctttttct aattgcttga
     3781 tttgactctc tattttactc aagcgatttt gcaaggcttt cccttttttc tgatcttcat
     3841 aagaagcttt actgcttgct ttttgatctt tagattgagc agaagctagt ttatcgctat
     3901 cttttttctc gacttcacgc atgttttcaa gattgcgttg ctctaagaaa taattaatat
     3961 cacctaaata ttctttgatt ttttggtctt tgaattcata aacaatattc gacatacctt
     4021 gaagaaaatc tctatcgtgt gaaaccaaaa gcaaagttcc ttcatatttt tgcaaggcag
     4081 ctttcaaaac atttttggat ttaatatcca agtgattcgt aggctcatcc attaccaaaa
     4141 cattgatggg ttgcaaaagc aatttgcata acgccaaccg atttctttcg cctcccgaaa
     4201 ggactttcac ctttttctct acttcatcgc cacgaaacaa gaaagaacct aacatatcac
     4261 gaacttttga acgattagag tcagaagctg cattttccat ggtttgcaac aaagtaattt
     4321 ctccatccaa atattctgct tgattttggg caaaataacc tacctgaaca ttgtgtccca
     4381 gtttgatgct tccttgaaat tcaaattcat taacaatggc tttaataaaa gtcgattttc
     4441 cttgaccatt ttgtccaaca aaagcaattt tactacctcg ttcgaccaaa agggaaatgt
     4501 ctttcaaaat aactctgtca ccataagctt tggtcacatt ttcggcttca attaccactt
     4561 ttccgggtac ttttgaaact ggaaaagaaa tattcatcac tgaattatca tcttcatcca
     4621 cttcgattcg ttctacttta tctaattttt tgataagcga ttgtgccatc gatgctttag
     4681 aagctttggc acggaatttc tcaattaact tttcagtttc ttcaattttt ttggcttgat
     4741 tcttctgagt tgccaattgt ttttctctaa tttcacctct caattccaaa tattcagaat
     4801 aaggcttgtt gaaatcgtaa gcttttccta gagaaatttc tatcgttcta ttcgtcacat
     4861 tgtccaaaaa cattttatcg tgcgaaacaa tcacaaccac tccaggataa ttgcgcaaga
     4921 aactttctaa ccaaataata ctctctatat ccaagtgatt ggttggctca tccagcagca
     4981 aaacatcgtt ggcttgtaat aataatttag ccaattcgat acgcattctc cagcctcccg
     5041 aaaaagtatc ggtttgatcg ttgaaaactt ctcttttgaa acccaatccc agaagaattt
     5101 tctcagtatc tcctatataa ttataacctc ccaacaattc aaaacgatgg gtatagtcgg
     5161 ataaatcttc gatgatttgg ctgtattcct cgctttcata atcagttcta gtgactaatt
     5221 gatgattgat ttgttctaat tttttttcaa caattttaat atcagtgaat gcttcgtagg
     5281 cttcttcaag aacagttctt ccctgttcaa aatcaatgtc ctgacgcaaa aaccccattc
     5341 gaacctcttt ttcttgagaa atcactcccg aatcaggagc aaaatctttg gccaagattt
     5401 tgagcattgt cgattttcca gcaccgtttt tcccgacaag ccctactcgg tctccggcac
     5461 ccaatctaaa agttacttct tcaaataaat aagttcctcc aaaagaaacg gacaaattgt
     5521 gtatattaag catgttttgt tactaatgtt acattataga aaggacaaaa ttgtaccttc
     5581 gctgaaaatt tttgcaaatg ttaaaaaaag gatccaaact aaatagtatt ttaacaggaa
     5641 gttgtcctag atgtcagaat gagagtatgt attcggataa aaatccgcta catttttcta
     5701 aaatactcca aatgaatgaa aattgtagtc attgtggact tcaatatcaa attgaacctt
     5761 cgttttttta tggagctatg tatgtaagtt atgcattgaa tgtagcactt agtatagccg
     5821 tatttgtaat ttcttatctc ttttttaatt cgagtataaa aacttcattt attgccataa
     5881 ttctagcaaa tattgtttta ttcccatttg ttttgagatg gtcgagaaat atttatatca
     5941 atttgtttgt gaagtttgac aagaagtata aaaaataaaa ttcactattt tttataaaac
     6001 ctttgaatat cgatttccct atccaaaggc ttttgaaatt caatattttc gaataatgct
     6061 ttggccaacg cagggccaag cattacacca cgagttccta atccgttgag gatatgaata
     6121 gaattgtgtt ttgaatgtgt tcctactagc ggtcttctgt cttttacagt tggacgaacg
     6181 cctgccaaat gttcgacaat ttcgaaatcg caattcagta tttctttaat tttttcgatt
     6241 aattcagtct ttccttcttc agttggaaca tcggttttgt cagcccaatt ataagttgct
     6301 cctactttgt ataaatcatt gccaagaggc aaaataaaga cactagtatt gattattaca
     6361 tccaagtcta atgctgctgc tttgataata aaaagctcgc cttttgtacc atctaaaggc
     6421 aaatctttga aataaggatt ggcatgcatt ccgaaacctt cggcaaaaat gatgtttcta
     6481 gcactaattt ctttatattg aatactgttg ccttcttctt gtaaaagaga ataatcgaaa
     6541 gattcttcta gaaaccaatt gttttcgatt aaatactctt tgtatttctt taacaacaat
     6601 gctgtatcaa cataaccagt ttgcaagact tcgccatatc caaaaggaga atctatccct
     6661 gcatactttc ttgaaataat attagttgac aaaaaaggag ccaaattcac tttatcagat
     6721 gcaaagaacc aattgttttg ctcttcgatt gaaaaaaatt tccttaaaat aggcatttca
     6781 aaattcactt tacaatttag ttttttttct aaattggaga aaaaatcatt cataatgggt
     6841 aaatgatttt tagcctgaga tacttcggta aatcttttta gaataacagg attatacaaa
     6901 ccacccgcaa tttcagatga gttttgagaa ttattatcta aaaccaaaat tgatttattg
     6961 ttttgcaaag ctatttcaga gaaagaaata cccgctaatc cagaacctac tattaaataa
     7021 tcgatcattt gaagtggaag ttaaaagtgc aaaaacaaaa aactcttacc ttgaagtaag
     7081 agtttttgtt ttatgataat aagaaagttt agtaattcca catatcttct tcaaaatttc
     7141 gaatcttatc ttttactctt tcagactcta ataattgatt ctgtgcatta tctttcatat
     7201 aacttgtaat tgccctatcg ccatacacat tttcttcttg ataaataacc gcattaaatc
     7261 ttcttgaatt taaaatctgg tcaaaagaaa cgggcattgc cgaattttta ttgttgaatg
     7321 ctttggcttc gtgcaacaca tctcttgaag ctgggaaaaa cacccaaaac aattcgatat
     7381 aatctttttc ttcactattc atagtgtaaa catcaggagt tactggacaa atacctagca
     7441 atctgtattt caattcactt tgacgtttat caaaatacca atatccttta attttatact
     7501 gggtaacatc ttgagccgta aggtcttgtt ttacaatata ttcaggcgaa attttttgac
     7561 ctgcattaat ttgttctcta ccagcatctg tggtatcaat ccttgtcaaa gaagcttgaa
     7621 tatctttata tgacttttta gtattgaaat aactatccga atagacttcg gtcagttctc
     7681 cgttttttat agctttggtc aatacatcat acaacgagcg tctgtcagac ccaatgtttg
     7741 ctgtatcaat tggaaaatac aaaggaaaat taattctttc gcttaaatca ataatttccc
     7801 aaaccgtttt ccccatcaat acatctctat catccacata accatattct aatggtttgt
     7861 cattgtctga aatgagttgt gcagcagact ttagtccaat ttcttcaggt atttttgcat
     7921 ttagcaaatt cggttgcgca tatgttgcaa cagtcccaaa aatggaaata acaactatta
     7981 aaaaatttct catattcatc atattatcat tgtaagctat tgaagtaact ctaaaacctc
     8041 ttatttattg tatttcataa attactggtg cagttcttgg caataaataa ctacctgctc
     8101 caactaattt agttttaatt tctgaaatag taacttgatc tcctcttccc actttggaaa
     8161 gaacagattt acattgtgca ttcaatttgt ttccaactac aaccaccgta ggttgtccag
     8221 ttactttaaa attgaatcca acaacatcta aaccaacttc aaagtcaaag tcaactaatt
     8281 tagctccaac agttgaaatt tcaagacttg atttaggtcc ttttaccaca cccatttctc
     8341 ctctaattgt tcctgttggg cctggaattc ctttgattct aaacgatttt ttatcagaaa
     8401 ctttagaacc atcaggtaat gttcctgtaa cgttgataac cacttcggtg cctgcacctg
     8461 gtcgcattgt ataagaactt gtattaccaa cacgtgttaa acctgccgca gcagctgaaa
     8521 ccttatcagg agaaatacct gcaaatgaaa ttgtcattgg gtttacaaca ccacgatata
     8581 caacattcat tttatctgct gatattgtag ctgattttgg tctggctaca actacatatt
     8641 tatctttaat tggcaaacta ataacttttc cgttttcgtc aaagttgaaa gaaccactaa
     8701 ttgggtgttc tccaatattt ccagatccga atgaaaaatt agcttgacca gcttgagctg
     8761 caacactaga accgtttaca acaacggatt tcgcttttaa tgaaggatca aatttaccca
     8821 agataatttt acctttaacg gcttcaccct gaaagaaaac agatttttct aaaactacaa
     8881 ttggttgata ggctgtaagt gacgctgcag caaccaagtc agattgaaac attccagtca
     8941 ttacatcact ctcagtagtt ttgatatcgg cttgcaattg cgacaattta gtaatggtag
     9001 caattaatgg aaatcctttg tagttataat caatccaatc caatttagct cccgcttttt
     9061 cagaataaac ttgatttgta gcaaatcttt tctctatttt tttcatttca gactcagcaa
     9121 tagaactacc tccaattgct tttatctgag ctgggtaact attgattttg tctaagaatt
     9181 ctttaccttg tttagacact ttgtctccag taaaaaacat tctgtctatc aaatcgcctt
     9241 tgtccatttc ttcgtaagga agatttcctt cggcatctct ttcaaatttt gcagtaacag
     9301 tagtttttat tccttctatg taatcgttaa attctttaga taaagctcta attttttcaa
     9361 cttttgctct tttatcgcca aattgacctg gttgatctac tgctttttgg gacaattgtt
     9421 gaaatgaact ttcatttcta ccatcagtaa tagaattcga ttcaataatt ttattgttca
     9481 aaattccaaa tgctgataat acttctttgg acatatttaa tgctaacatc gcgataaaaa
     9541 ccaagtacat taggtttatc atcttctgcc tagggcttaa ttttcctcct gccatatttt
     9601 ctaataatta gttgttgttt ttataaaaag tgggaactaa ttatcctttg ttactcattg
     9661 cagaaagcat tcctccataa acactgttca atgaagacaa gtttgaagtt aatgattgca
     9721 tttgttcttt taattttaaa ttgttttcag ccacttcttc attgatttga gcatttcttg
     9781 aagcgctttg caattgaact ttgtataagc tatttaaaga ttccatttga gctgctgcca
     9841 aagttaattc ttcactatat ttttgagtag atgcaataga atctacagtt ggcgaaatgg
     9901 cttttgctgc tgattcgaaa tttttaatac tatttcctaa acttgacatt aattcaccat
     9961 caattttagc gtcttttagc attgcatcca attttttaga caacattcct tgagcatctt
    10021 cggtaggaac ttcagttttt gctacttttt ttctagcctc accgttagcc aattctggat
    10081 aaactaatgt ccaatctaat tcattttcta cgggttcaaa agcagaaaga gcaaaaatta
    10141 aagcttcaac tactagacca atggtaagca ttaaatttcc agttataaaa cccaactcaa
    10201 tgtgagttat tttgaataaa gctccaataa ttactactgc tgctcccatt ccgtatgcaa
    10261 aattcatctt ttgtttactt atcaatgcca taatctttgt tttttaggtt aatttattta
    10321 aatttggttt tttatttttt ctttttgctt ttttctgttg actgtgttcc catataatcc
    10381 tgaactgttc taaaacctat aaaacttctt gctgaatcag catattcaaa agttctagta
    10441 cccacttgaa ggaaataggc aacatctttc caagaaccac cacgaactac tttacgcttg
    10501 tttgaagtgt cagtattatt tggattcata gaagaaacat attc
//
LOCUS       DAOSNN010000063        10472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6355_length_10472_cov_5.542383, whole genome shotgun sequence.
ACCESSION   DAOSNN010000063 DAOSNN010000000
VERSION     DAOSNN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6355_length_10472_cov_5.542383"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLQ70_04790"
     CDS             complement(<1..299)
                     /locus_tag="PLQ70_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQE33965.1"
                     /translation="MQKIISYPISFIAILLFLLTLVVFHPIQWICFNVFGYQAHKKSV
                     DYLNFFLLRIGHIMFNTFKIEGRENIPTGVPIIFVANHQGMYDIIGMIWFLRKFHP"
     gene            complement(390..1124)
                     /locus_tag="PLQ70_04795"
     CDS             complement(390..1124)
                     /locus_tag="PLQ70_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQE33966.1"
                     /translation="MKKILLYPITLFYYFVYMVVLCTIQPLQWLAFKIFGDKGLKISF
                     DYVAFFSLKCNNILGTRFTFENRELIPKGVPVIFVANHQSLLDTAGIIWYLRKFNCLF
                     VTKQELAKGIPSISYFLRNGKYVCIDRSNPKQAIPKIKSLAEYIEKENYSAVIFPEGT
                     RSKTGIPNTFAESGLKILCKYAPSAYIVPITINNSWKVYQYGYFPLSVGHHLFFTVQK
                     PLKVSDYSFEEILKKTEKSIIDGIKN"
     assembly_gap    1335..1434
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1435..1686
                     /locus_tag="PLQ70_04800"
     CDS             <1435..1686
                     /locus_tag="PLQ70_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33967.1"
                     /translation="ILNLSDFKIFYIFVLLKITTMATITLSYDARNVIAKKTVAYILS
                     LGVFKTINKTAIDLSLEDVKKGRVTTHKSAADYFKNLKD"
     gene            1686..1958
                     /locus_tag="PLQ70_04805"
     CDS             1686..1958
                     /locus_tag="PLQ70_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HQE33968.1"
                     /translation="MYIVKTTRQFDKEVKLCKKRGYDLSKLEEVVSILEKTGTLPQKY
                     KPHKLSGNYENCWECHIKGDWLLIWLQNDTELTLLFINTGTHNDLF"
     gene            2023..2427
                     /locus_tag="PLQ70_04810"
     CDS             2023..2427
                     /locus_tag="PLQ70_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQE33969.1"
                     /translation="MKFTYPKNEKLKSKITIDLLFSKGKSVSKYPLRLVFVESDYGIP
                     ESREFGTDQKLKMGVSVSKRNFKHAVDRNYFKRVLRETYRLNKHILIDNLDKNYAMMF
                     FYQTKDRLSYEEINTKTIQLFEKFVQQLKNEE"
     gene            2502..4139
                     /locus_tag="PLQ70_04815"
     CDS             2502..4139
                     /locus_tag="PLQ70_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQE33970.1"
                     /translation="MIPFYKKRYIFPVIASAFLFVGVSFKDDFFEIAKQIEIFTTLFK
                     ELNKNYVDETNPGDLMDKSIKAMLASLDPYTVYFNEQDVVKYKINNTGEYTGIGAMIT
                     RKNDKLIIREPYKNFPADKAGLKAGDEVIQIGDILLSDFKEDASQLFRGSKNTKIDVK
                     YIRQGKTNTTQIVLDEVEIKSVPFYGKIDDKTGYIVLARFNQKASYETKQALEQLKKE
                     GAERIVLDLRGNPGGLLNEAVNICNLFVAKGEIIVTTKSKNEKYNTTYKTSKEPVDIE
                     IPLAILVNGRSASASEIVSGALQDLDRAVVIGSRSFGKGLVQKPIDLTYGTQLKVTIS
                     RYYTPSGRCIQALDYAHKDKNGVASKTEAKNYTAFKTRKGRTVYDGGGIQPDIELEET
                     KWSPIAEALQKNDGIFNYATSYYYKNPNLGNQIPTFSDADFQNFKQFLKTEKFSFDTE
                     TDLALKNTLAKAKKEKIDENIAAEYQQLAIALQKSEENLLDKNKAEIKNLILDEIIKR
                     YQYQEGLYQYYLKNNAEIKKATSVLNNSSEYKNILKM"
     gene            4139..4930
                     /locus_tag="PLQ70_04820"
     CDS             4139..4930
                     /locus_tag="PLQ70_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQE33971.1"
                     /translation="MKRSLQIVLLLFFSSLVAQINTAQSVYFEFDKFTLNKNDLSPII
                     TLINSPNFSQFEAIQLYGYCDDRGSEAYNDKLSKRRVDFIQKLLISNGISQNKIFICE
                     GRGKVNLDIDTTKNLKEVRDKNRRVDLIFIKKSTYTSFPENPKVGDNIILNQVLFEMG
                     SSELSLTAKKELDRIAVLLQKHKNLRFEIKGHVCCTSNKYKDAIDKEDQVRNLSENRA
                     KNVFLYFRSKGISPYRMSFKGYGNLFPLGKEDAQDRRVELYITKL"
     gene            complement(4953..6560)
                     /locus_tag="PLQ70_04825"
     CDS             complement(4953..6560)
                     /locus_tag="PLQ70_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQE33972.1"
                     /translation="MKRRSFIKTSTLATIPLALNGIPVMASSSIESNELDILANAAVS
                     CGKILVIIQMNGGNDGLNTVFPLDKWTNLNNARSNILMNQSSVLPLANNTTTGIHPAM
                     SDLKNLHDNGKLMIVQGVSYPNPSYSHFRATDIWFTASNSNQDLDSGWLGRSLDKIYP
                     GYPIGYPNSDMPDPLAIQIGSTLPFSLQGPNINMGYSVPDPNSLLNVINQITDPAPNS
                     DYGAELTFLRLMKDQSNVYRAAIQNAFNTTQAQSATYPSSNKLGDQLKIVAKLINGGL
                     QTPIYIVNHPNTSDLHSAQVDVTDKTQGAHANNLSILSQAINAFQQDLELMGKSNLVT
                     GMTFSEFGRRIKSNGSLGTDHGAGAPVIFFGAALNTGPSSITAQNPVSGMIGSSPNLP
                     LNATTSDQVAMQFDFRQIYSSIMQDWLCMSESQTTDVLGGTFAKLALFKNTALDTVSF
                     ESDTFFSVYPNPITENTININFKDSLNGYVNVQLFTIQGTKIFNKAFRVNGNTLKFSP
                     DYSMASGIYILELEYNTYKFHTKVIVS"
     gene            complement(6572..8392)
                     /locus_tag="PLQ70_04830"
     CDS             complement(6572..8392)
                     /locus_tag="PLQ70_04830"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HQE33973.1"
                     /translation="MSNTINRRNLFKSVLNKFKKNQDSGDPLFEKYSRKIFNGRRYQS
                     NTKSKNSGFTDRVVPVSSGLSPYTGIWEKSQVLHLLRRTGYGFKKSDVDTLLALSMTN
                     AVNAVLNVNTTPPSPPVNSYNNYSPDESSLPYGADWTTNPLPSGTNTTNSRRIDSLSQ
                     WNFGLACNQDLTIKEKMTWFWYHFIPVDFEIVRTAPFSYASSNSCRILYTYMKMFRDN
                     PLGNFKTLIRTMATQPAMMYYLNNQANTKTSPDENFAREIMELFTLGKDPLSQYTEVD
                     VVQAAKVLTGWRVVNLNTATPSTNFDNTYHETSNKQFSSFFNNTIINNAGASELDAFI
                     NMIFTKSQVVSEYICRRLYRYFVYYDIDATIESTIITPLAQTFVANNWEIAPVLDQLF
                     KSQHFYDMANRGVYIKSPFDLVIGSLRTFNLNYTVADQTNFEAQYKVWNYYNNSISSG
                     LEQTMGRIPNVSGWVAFYQNPSFHEYWINSNTTQKRFDFLNRIFNGYNLTYNLLTTRI
                     EVDLIAFIQQFDPAICEDPDLLVAECIAYLLPIDLSATQKEQIKIQSLLSNQTTNSYW
                     TTAWSLYLSNTSNTTYKNSVKTRLKSLLVTLTQLAEYQLM"
     gene            complement(8557..9006)
                     /locus_tag="PLQ70_04835"
     CDS             complement(8557..9006)
                     /locus_tag="PLQ70_04835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE33974.1"
                     /translation="MSSLQWKIKSFENLSVNELYDILRLRSEIFVVEQNCVYLDLDGK
                     DKLSLHLFGEFDGKIVAYSRLFKPGITFENASIGRVVVDANYRDKKWGHDLMRESIAA
                     IKSHFGESKITIGAQLYLKKFYESHGFVQTSEMYLEDDIPHIEMKKE"
     gene            complement(9049..10026)
                     /locus_tag="PLQ70_04840"
     CDS             complement(9049..10026)
                     /locus_tag="PLQ70_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HQE33975.1"
                     /translation="MNKNFFLFLSAIALLFFSCKKQEDNLPRKATGKINTISVIIDDQ
                     LWNGEIGESIRNKFASPVIGLPEEEPLFTINQYPVKLMEGFMTDSRTIIVVKKDTKTK
                     FEIRKNQYASPQNVFHISGKTNGEILELIEKNASQIIQIIKETEIAESQRINRLSLMN
                     PVIIRNKFQISLQVPYGYLYALQSSKFLWLKKEIISGNTSILIYEVPINTIKNSSNII
                     ENIIKTRDSIGNLYIRGTEADTQMITEEGYSPYFFKTKINGKEAFETKGTWELKNDFM
                     SGPFVNYAIIDQKRNRVLILEGFCYSPSKEERDLIHELEAVIKSIVILN"
     gene            complement(10109..>10472)
                     /locus_tag="PLQ70_04845"
     CDS             complement(10109..>10472)
                     /locus_tag="PLQ70_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HQE33976.1"
                     /translation="YNIPTDAPDNIWPKGIQFQIKENTTGDFIFLDKITAVVNGKLVE
                     AGASVTSAKFSENEKPYGEWNSIVIISYNGKITQFLNGKLINECVEASSIEGKISLNY
                     EGSPIDFRNIQIKNISKE"
BASE COUNT         3462 a         1738 c         1756 g         3416 t
ORIGIN      
        1 ggatgaaatt ttctcaaaaa ccaaatcatt ccaataatat catacattcc ttgatgattg
       61 gccacaaaaa tgataggaac tcccgttgga atattttctc ttccttcgat tttaaaggtg
      121 ttgaacataa tgtgtcctat tcgtaacaag aaaaaattca aataatcgac acttttcttg
      181 tgggcttggt atccaaaaac attgaaacaa atccactgaa tgggatgaaa tacaactaat
      241 gtcaacagaa ataatagaat tgcaataaag gatattgggt aggatatgat tttttgcatt
      301 aggtgttttt tttggtaaaa ataggaaata tatttttgag cgttagatgg gtttattgat
      361 ttaaatttaa tgatttttta gattcaaaat taattcttta ttccatcgat aatcgatttt
      421 tcagtctttt tcaatatctc ctcaaaagaa taatcgctca cttttaaagg cttttgaacc
      481 gtgaaaaaaa gatgatgacc aacacttaaa ggaaaataac cgtattgata cactttccaa
      541 gaattgttga tggttatggg aacaatatag gccgatggcg catatttgca caatattttc
      601 aatccgcttt cggcaaatgt attaggaatt cctgttttgc ttctggttcc ttcaggaaaa
      661 ataactgccg aatagttttc tttttcgatg tactctgcca atgattttat tttgggaatg
      721 gcttgtttgg gattgcttcg gtcgatacac acatatttcc cgttgcgcag aaaataggaa
      781 atactcggaa ttccttttgc taattcctgt ttggttacaa aaagacaatt aaattttcgc
      841 aaataccaaa taattccagc agtatccaac aaactttggt gatttgccac aaatataacg
      901 ggaacgcctt ttggaatcag ctcccggttt tcaaaagtaa atcgagttcc taaaatatta
      961 ttgcatttta aactaaaaaa agccacataa tcaaaagaga ttttcaatcc cttgtcgcca
     1021 aaaatcttaa aggccaacca ttgaagaggc tgaatcgtac acaaaaccac catatacaca
     1081 aaatagtaaa atagtgtaat gggatataat aaaattttct tcattcgatt tttagattac
     1141 acgcaaaagt aataattatt tttgtcttca attttaatcc caaacattta tccgtttagg
     1201 gtttaattgt acatttgctc tggaaaatct ttgctatttt tcaaaaaaca catgaaattc
     1261 acttatccaa aaaacgaaaa ttctcaatct atcggatttt aaaatatttt atatctttgt
     1321 actattaaaa attannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnattctc
     1441 aatctatcgg attttaaaat attttatatc tttgtactat taaaaattac tactatggca
     1501 acaataacat tatcatatga cgctcgtaac gttatcgcaa aaaagacagt tgcttacata
     1561 ctttcgttgg gcgtttttaa aactatcaat aaaactgcaa tagatttatc tttagaagat
     1621 gtgaaaaaag ggcgtgttac tactcataaa agtgccgcag actatttcaa aaatctaaaa
     1681 gattaatgta cattgtaaaa actacaaggc aatttgacaa agaagtaaaa ctttgtaaaa
     1741 aaagaggtta cgatctttct aaattagaag aagtcgtttc tattttagaa aagacaggaa
     1801 cactacctca aaaatacaaa ccacataagt tgtcgggaaa ttatgaaaat tgttgggaat
     1861 gccacatcaa aggcgattgg cttttaatct ggttacaaaa cgacactgaa cttacccttt
     1921 tatttatcaa tacaggaacg cataacgact tattttagac taactatcga agattataaa
     1981 tcgaatttgt ccctaaaatc tttgttattt ttcaaaatat aaatgaaatt cacttatcca
     2041 aaaaacgaaa agctaaaaag taaaattacg atagatttgc ttttttctaa aggcaaatcc
     2101 gtgtccaaat atcctttgag actagttttt gttgaaagtg attatggcat tcccgaatcc
     2161 cgagaattcg ggacagatca aaaactaaaa atgggagttt cggtttccaa aagaaatttc
     2221 aaacacgcag ttgatagaaa ctatttcaag cgggttcttc gcgaaaccta tcgattgaac
     2281 aaacacatcc taatcgataa tttggacaaa aattatgcga tgatgttttt ttaccaaacc
     2341 aaagaccgat tgtcttacga agaaatcaat accaaaacca ttcaactgtt tgaaaagttt
     2401 gtacaacagt tgaaaaacga ggaataacga ctttgttttt tacttgaatg tttaaattgc
     2461 ttaatatttc ctattttagt ggtctaaaat taagccctat tatgattcct ttttataaaa
     2521 aaagatacat ttttcctgtt atagcctcag catttttgtt tgtaggcgtt agtttcaaag
     2581 atgatttttt tgaaattgcc aagcaaatag aaatattcac gacacttttc aaagagttaa
     2641 acaaaaatta tgttgacgaa accaatccgg gtgatttaat ggacaaatcc ataaaagcaa
     2701 tgttggctag tttagaccca tataccgttt attttaacga gcaagatgtg gttaaataca
     2761 aaataaacaa cacaggcgaa tataccggaa ttggcgcgat gattactcga aaaaatgata
     2821 aattaatcat tcgtgaaccc tataaaaatt ttcctgccga caaagcgggt ctcaaagctg
     2881 gtgacgaggt gattcaaatt ggtgatattc ttttgtctga cttcaaagag gatgcttcac
     2941 agctttttag aggttccaaa aacactaaaa ttgacgttaa atacattcgc caaggcaaaa
     3001 ccaatactac tcaaatcgtt ttggacgaag tagaaatcaa atcggtgcct ttttatggca
     3061 aaatagacga caaaacaggc tatattgttt tggctcgttt caatcaaaaa gcttcttatg
     3121 aaaccaaaca agctttagag caattaaaaa aagagggagc cgaaagaata gttcttgatt
     3181 tgagaggcaa tcctggagga cttttgaacg aagccgtgaa tatctgcaat ttatttgttg
     3241 ctaaaggtga aattattgta accacaaaat cgaaaaacga aaaatacaac accacttata
     3301 aaacgtctaa agaacctgtc gatatcgaaa ttccattggc aatcttagtc aacggaagaa
     3361 gtgcttcggc ttcagaaatt gtttcgggag ctttgcagga tttagatcgc gcggtagtga
     3421 ttggcagtcg aagttttggt aaaggattgg tacaaaaacc tattgatctg acctatggaa
     3481 cgcaattaaa agtaacgatt tcgcgctatt acactccttc tggcagatgt attcaagctt
     3541 tggattatgc tcataaagac aaaaatggtg ttgcctcaaa aactgaagca aagaattata
     3601 ccgctttcaa aacccgaaaa ggcagaacgg tttatgatgg cggaggaatt cagcccgaca
     3661 ttgaattgga agaaaccaaa tggagtccaa ttgctgaagc tttgcaaaaa aatgacggga
     3721 ttttcaatta tgccacttct tattattaca aaaatccaaa tttaggcaac caaatcccaa
     3781 ccttttcgga tgccgatttt caaaatttca aacaatttct aaaaaccgaa aaattctctt
     3841 ttgacaccga aactgatttg gctttgaaaa acacattggc taaagccaaa aaagaaaaaa
     3901 ttgacgaaaa tattgctgca gaataccagc aattagccat tgctcttcaa aaaagtgagg
     3961 aaaatttatt ggataaaaac aaggctgaaa tcaagaattt aatccttgac gaaatcatta
     4021 aacgctatca atatcaagaa ggcttgtatc aatattactt gaaaaataac gccgaaatta
     4081 aaaaagcaac tagtgttttg aataatagtt ccgaatataa aaacatatta aaaatgtgat
     4141 gaaaaggagc ctacaaatag tgttgttatt gttttttagc tcacttgtag cgcaaatcaa
     4201 cacggctcaa tcggtgtatt ttgaatttga taaattcacc ttaaataaaa acgatttatc
     4261 acccattatt accttaatta attcgcctaa tttttcacaa tttgaagcga ttcagcttta
     4321 tggttattgt gatgacaggg gaagcgaagc ctataacgat aaattatcca aacgaagagt
     4381 cgattttatt caaaaattat taatctctaa tggaatttct caaaataaaa tattcatttg
     4441 cgaaggccga ggaaaagtaa atctggatat tgacaccaca aaaaatttaa aagaagtaag
     4501 agataaaaac cggcgtgtcg atttgatttt tattaaaaaa tcaacctata cttcctttcc
     4561 agaaaatcca aaagtgggcg acaacattat tctaaatcaa gttctgtttg aaatgggttc
     4621 gagtgaactt tctttaacag ctaaaaaaga attagataga attgcagtgc tgttacaaaa
     4681 acacaaaaac ctgcgttttg aaattaaagg ccacgtttgt tgtacatcaa acaaatataa
     4741 agacgcgatt gacaaagaag accaagttag aaatttgtct gaaaatcgag ccaaaaatgt
     4801 gtttttatac tttcgttcca aaggaataag cccttatcga atgtcgttta aagggtatgg
     4861 aaatctattt cctcttggaa aagaagatgc tcaagaccgt cgtgtggagc tttatattac
     4921 aaaattataa taggttttct taagttttta catcacgaaa caatcacttt ggtatggaat
     4981 ttataagtgt tgtattctaa ttctaaaatg taaattccgg atgccattga ataatcagga
     5041 gagaatttta aggtattgcc atttactctg aatgctttat taaaaatctt tgttccttga
     5101 atagtaaata attgcacgtt tacataccca ttcaaactgt ctttaaagtt aatattgatt
     5161 gtgttttcag taattggatt tggataaacc gagaaaaaag tatcactttc aaacgaaact
     5221 gtatctaaag cggtgttttt aaatagggcc aattttgcaa aagtacctcc taaaacatcg
     5281 gtagtttgag attcactcat acagagccaa tcttgcataa tcgaagaata aatttgtctg
     5341 aaatcgaatt gcatcgccac ttggtcagaa gttgtagcat tcaacggcaa gttaggagag
     5401 cttcctatca tccccgaaac agggttttgt gcagttatag aacttggtcc ggtattcaaa
     5461 gcggctccaa agaaaattac tggtgctcca gctccgtggt cagttcctaa acttccgttg
     5521 ctttttattc ttcgaccaaa ttcactaaag gtcattcctg tcaccaaatt cgatttaccc
     5581 ataagctcca aatcttgttg aaaagcattt atggcttgag ataaaatact taaattattg
     5641 gcgtgagctc cttgggtctt atcagtaaca tctacttgag cgctatgcaa atcagaagta
     5701 ttgggatgat tgacaatata aattggagtt tgcaatccgc cgttgattaa tttggcaaca
     5761 atttttaatt ggtcgcctaa tttattcgaa cttggatagg ttgctgattg ggcttgtgtg
     5821 gtgttaaaag cgttttgaat ggctgctcga tatacattac tttggtcttt cattaaacga
     5881 agaaaagtta actcggctcc ataatcagag ttgggtgcgg gatctgtgat ttgattgatt
     5941 acattcaaaa gcgaattggg atctggaaca ctatagccca tattgatatt tggcccttgt
     6001 aaagaaaaag gcaaggttga accaatttga attgccaacg ggtcaggcat atcgctatta
     6061 ggataaccta taggataacc tggatatatt ttatctaaag atcttcctag ccaaccgctg
     6121 tctaaatctt gattactatt cgaagccgta aaccaaatgt cggtcgctct aaaatgactg
     6181 taactcggat tgggatatga taccccttgt acaatcatta acttaccgtt gtcgtgcaga
     6241 tttttcaagt ctgacatagc cggatgaatt ccagtagtgg tgttattagc caatggtaaa
     6301 accgaacttt gattcattaa aatattagaa cgggcattgt tcaaattcgt ccatttgtct
     6361 aatggaaaaa ctgtgtttaa tccatcgttt ccgccattca tttgaatgat taccaatatt
     6421 tttccgcaac tcaccgctgc atttgccaaa atatccaatt cattactttc aatagaggag
     6481 gatgccatta caggaattcc gtttaatgcc aaaggaattg tggccaatgt acttgttttt
     6541 ataaaacttc ttcttttcat gacttcaaag gttacattaa ttggtattct gcaagttgag
     6601 taagggtgac aagcaaggat tttagtcttg ttttcacact gtttttataa gtcgtattcg
     6661 aagtgttgct aagataaagc gaccaagcag tagtccaata gctatttgtg gtttgattgg
     6721 aaagtagtga ttgtatttta atctgttctt tttgagtagc gcttaagtca attggcaata
     6781 aataggcaat acattctgct accaataaat cgggatcttc acaaatagca gggtcaaatt
     6841 gttgtataaa ggctatcaaa tcaacttcga tacgcgttgt aagcagattg taagtaagat
     6901 tgtaaccatt gaaaattctg tttaagaaat caaatctttt ttgagttgta tttgaattta
     6961 tccaatattc atgaaaagag ggattttgat aaaaggcaac ccaacccgaa acattgggaa
     7021 ttcttcccat agtttgctct aaaccgctag aaatactatt gttgtaataa ttccaaactt
     7081 tatattgcgc ttcaaaattg gtttgatctg caactgtata attcaaattg aacgttctca
     7141 aactaccaat caccaaatcg aaaggcgatt tgatgtaaac gcctctattg gccatatcat
     7201 aaaaatgttg gcttttaaaa agttggtcta atacgggtgc gatttcccaa ttgttggcta
     7261 caaatgtttg tgccaatggt gttattatag ttgattctat tgtagcatca atatcataat
     7321 agacaaaata acgatacaaa cggcggcaaa tatattctga aaccacttgc gatttcgtga
     7381 aaatcatatt gataaaagca tctaattctg aagctcctgc attgttgata atggtgttat
     7441 tgaaaaatga ggaaaattgt ttgttactcg tttcgtgata agtattatca aaattagtac
     7501 taggagtagc ggtatttaaa ttgacaaccc tccatcctgt taaaactttg gcagcttgca
     7561 ccacatcaac ttctgtgtat tgacttaatg ggtcttttcc taaagtaaaa agttccatga
     7621 tttcacgagc aaaattctca tctggtgacg tttttgtatt ggcttgattg ttcaaataat
     7681 acatcatcgc aggctgagtt gccatagttc ttataagtgt tttgaagttt ccaagaggat
     7741 tatcccgaaa cattttcata taagtgtaca atattcggca agaattagaa gacgcatacg
     7801 aaaatggagc cgtacgaacg atttcaaaat ctacaggaat aaaatgatac caaaaccaag
     7861 tcattttttc cttaatagta aggtcctgat tgcaagccaa accaaaattc cattgcgata
     7921 atgagtctat tcttcttgaa ttagtggtgt ttgttccaga aggcaatggg ttggttgtcc
     7981 aatcagcacc gtaaggcaaa ctactttcat ctggtgaata attattatac gaatttacag
     8041 gcggagaagg tggcgttgtg tttacattta atacggcatt tacagcattg gtcatactca
     8101 aagctagcaa agtatcaaca tccgattttt tgaatccata tcctgttcgt cgcaacaaat
     8161 gcaaaacttg tgatttttcc caaatgccag tataagggct caatccgcta cttacgggaa
     8221 ccactctatc tgtaaaacct gaattttttg actttgtatt gctttggtat cgtcgtccgt
     8281 tgaatatttt tcgagaatat ttttcaaata aaggatctcc tgaatcctga ttttttttaa
     8341 acttatttaa taccgattta aataaatttc ttcgattgat agtattgctc atataaactt
     8401 cctaatttga tgtgtataat tcgactcaaa aaacattcaa tagtttaatc aaaaatatgt
     8461 tttttacaat acgttcaacg tactaaaaat ctgcggatta tagtatgcaa agaagcaagc
     8521 cataactagg cttttacaag attataaagc acttaattac tcttttttca tctcaatatg
     8581 cggaatatcg tcttctaaat acatttcgct ggtttgtaca aatccgtggc tttcgtagaa
     8641 ttttttcaaa tataattggg ctccaatggt gattttgctt tcgccaaaat gactcttaat
     8701 tgccgcaatt gattctctca ttagatcgtg accccatttt ttgtctcgat aattagcatc
     8761 aacaaccaca cgcccgatag aggcattttc aaaagtaatt cctggtttga aaagcctaga
     8821 atatgccaca attttaccat cgaattcacc aaaaagatgc agtgataatt tatcttttcc
     8881 atcaagatca agataaacac agttttgttc tacgacaaat atctcgcttc tcaatctcag
     8941 aatatcataa agttcgttta ccgaaaggtt ttcgaaagac tttattttcc attgtaggct
     9001 gctcataaat aaacgggttt atgttttatc aatgcaattg attttctctt agttcaaaat
     9061 aacaattgat ttgattacgg cttctaattc gtgtattaaa tcccgctctt ctttggacgg
     9121 tgaatagcaa aatccttcca aaattaatac tctgttgcgt ttctgatcta taatggcata
     9181 atttacaaat ggaccggaca taaaatcgtt tttcagctcc caagttcctt tggtttcgaa
     9241 agcttctttg ccgtttattt tggttttgaa aaaataaggc gaataacctt cttcagtaat
     9301 catttgagta tctgcttcgg ttcctctaat gtataaattg ccaatagaat cacgggtttt
     9361 gatgatgttt tcaattatat tagagctgtt tttaattgtg tttataggaa cttcataaat
     9421 caaaatactg gtgttgccac taataatttc ttttttcaac cacagaaatt tgctgctttg
     9481 aagcgcatac aaatagccgt aaggaacttg caacgaaatc tgaaacttgt ttctaatgat
     9541 aactggattc atcaaggaaa gtcggtttat tctttgactt tcggcaattt cagtttcctt
     9601 aatgatttgg ataatttgag aagcattttt ttcgattagt tctagtattt caccattggt
     9661 ttttcccgaa atatgaaaca cattctgagg cgaagcgtat tgattttttc taatttcgaa
     9721 tttggttttg gtgtcttttt ttacaacaat aattgttctg ctatcggtca taaatccttc
     9781 cattaatttt actggatatt gatttatggt aaaaagaggt tcttcttcgg gcaagccaat
     9841 aacgggtgat gcaaacttat ttcgaatgct ttcgccaatc tcaccgttcc agagttgatc
     9901 gtcaataatt acagaaattg tattgatttt tccggttgct tttcgcggca aattgtcctc
     9961 ttgttttttg catgaaaaaa acaaaagagc tattgctgat agaaataaaa agaaattttt
    10021 attcattgat acggaattgt tcctcaaata aagcgaaaaa aagacttgct tttataaaaa
    10081 gtgacaaaag tattttggca atttggcttt attctttaga aatatttttg atttgaatgt
    10141 ttctaaaatc aattggggaa ccttcataat tcaatgaaat tttaccttct atagaagaag
    10201 cttcaacaca ttcgtttatt aattttccgt tcaaaaattg ggtgattttt ccgttatagg
    10261 atatgattac aatggagttc cattcgccat aaggcttctc gttttcgcta aattttgccg
    10321 aagtaacact tgcgccagct tcaactaatt ttccgttgac aacggccgtt attttgtcta
    10381 aaaagataaa gtctcctgtg gtattttctt taatttgaaa ttgaattcct tttggccaaa
    10441 tgttatctgg agcatctgtt ggaatattgt ac
//
LOCUS       DAOSNN010000064        10465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6358_length_10465_cov_5.641883, whole genome shotgun sequence.
ACCESSION   DAOSNN010000064 DAOSNN010000000
VERSION     DAOSNN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6358_length_10465_cov_5.641883"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..643
                     /locus_tag="PLQ70_04850"
     CDS             65..643
                     /locus_tag="PLQ70_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE33977.1"
                     /translation="MKKSLLAIAIAILLTSCSPTLKVKQLDAKTGKLPTETTLTQSEI
                     LVKKNINLKEYNQFLFVKKSGLNMEKYETYILGTLKNIGGFQKYYIQTELEQYVIQNG
                     LTDKVTSISDNIGLLNLSKNVGKFLICESNVEYKGGYDFSFEYKVINPTNAEILLHIK
                     HNAFNWGGLDRPLFNPVFNEYIDWLKENQNQQ"
     gene            complement(748..1791)
                     /locus_tag="PLQ70_04855"
     CDS             complement(748..1791)
                     /locus_tag="PLQ70_04855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQE33978.1"
                     /translation="MSENNEEIVDLEDELFEHYRFDVPKGQEFLRIDKYLMNMIPNAT
                     RNKIQNAATGGDIYVNDIPVKSNYKVKPFDVIRIMLSHPPFENRVDPEDIPLNIVYED
                     DAVLLINKPPDFVVHPGHGNYTGTLVNALAFHFENLPMNSSERPGLVHRIDKDTSGLL
                     VIAKTEAAMTHLAKQFEAKTTEREYIALVWGNVIANEGTIEGNIARHLKDRMQMAVFA
                     DPEIGKPAVTHYKVLERFGYVTLVSCILETGRTHQIRVHMKHIGHPLFNDARYGGDLI
                     LKGTTFTKYKQFIENCFKALPRQALHAKTLGFMHPTTGEYMRFDTELPQDFQDCLEKW
                     RSYSKTHASEEEE"
     gene            complement(1788..2390)
                     /locus_tag="PLQ70_04860"
     CDS             complement(1788..2390)
                     /locus_tag="PLQ70_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HQE33979.1"
                     /translation="MSLRKYLTSRVFFGQAIAALAILAVLSYLFMHWLTFTTDHGHEI
                     TVPNLAKLTEEQVEEKLDELDLDYVLLDSVDYNSDYPKYSVVEQDPLPGAKVKEGRKV
                     YIKINASGFSSVKIPDLIDKTYREAVPTLKALGLEEGTITYIPNLGKDMVLEMRYKGR
                     NVKAGDRVLKASKIDLVLGDGKESYVEEVDSLAVQKDSIQ"
     gene            2661..3629
                     /locus_tag="PLQ70_04865"
     CDS             2661..3629
                     /locus_tag="PLQ70_04865"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQE33980.1"
                     /translation="MKNIAIIMGGYSSEYKISLISGNVVYQFLDKTKYNGFRIHIFKE
                     KWVYVDDNETEFPIDKNDFSTTVNGTKITFDCVFNAIHGTPGEDGLMQAYFELIGMPQ
                     SSCDYYQAALTFNKRDMLSVLKPYGIKTATSYYLNKGEHIDTAEIVKTVGLPCFVKPN
                     KSGSSFGISKVKTEAELPIAIEVAYQEDNEIIIESFLDGTEVSVGVINYQGKVIVLPI
                     TEIVSENDFFDYEAKYQGKSQEITPARISEEMTQKVSEAAKRAYEVLKMKGFSRSEFI
                     FVDGEPHMLEMNTIPGLTTESLIPQQAKAAGISLTDLFTNAIELAL"
     gene            3742..3966
                     /locus_tag="PLQ70_04870"
     CDS             3742..3966
                     /locus_tag="PLQ70_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HQE33981.1"
                     /translation="MIDYKQYIAINTQIRFGKPTIIGTRITVFDILYWMANGMSINDI
                     IEDFPELNENQIKACLAYSADREHKIQITL"
     gene            3963..4304
                     /locus_tag="PLQ70_04875"
     CDS             3963..4304
                     /locus_tag="PLQ70_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015362024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HQE33982.1"
                     /translation="MKLLLDQNISLRVISKIKNNFPEAKQVKELGIENYLDIEIWEFA
                     RDNKFTIVTFDADFYDLSHLNGHPPKIIWLRFGNTTNDLIASIINSRYSVINDFINAK
                     DYSEIVCLKIK"
     gene            4317..4895
                     /locus_tag="PLQ70_04880"
     CDS             4317..4895
                     /locus_tag="PLQ70_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HQE33983.1"
                     /translation="MNKSRLEAFSDGVLAIIITIMILEIKAPEDDSFESLQHLIPVFL
                     SYVLSFVYVGIYWNNHHHMFQVVKKVSGSILWSNLFLLFWLSLIPFATSWIGEHQFSA
                     VPMSVYGFVLLMCAVSYIILQNKIIKLEGNDSVLHRAVNSDTKGKISMACYISAIPLA
                     FVSPWISGLLYIAVALIWVIPDSRIEKQLNKL"
     gene            4897..5355
                     /gene="coaD"
                     /locus_tag="PLQ70_04885"
     CDS             4897..5355
                     /gene="coaD"
                     /locus_tag="PLQ70_04885"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQE33984.1"
                     /translation="MRKAIFPGSFDPITLGHEDIIRRGITLFDEIVIAIGVNAEKKYM
                     FTLEERKHFLEDTFKDEPKISIITYEGLTIDLCKKIGANFILRGLRNPADFEFEKAIA
                     HANRRMSKIETVFLLTAAKTSFISSSIVRDVIRHKGQYELLVPDAVRVKK"
     gene            5426..5986
                     /locus_tag="PLQ70_04890"
     CDS             5426..5986
                     /locus_tag="PLQ70_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HQE33985.1"
                     /translation="MERTLNKRSGSQCELCAATENLKVYTVLPTKKGGIDENILACPT
                     CIDQIENPGHEDQNHWRCLNESMWSEHIPVQVVSWRMLSRLRNNELVEQMYLDEDTLV
                     WAQATGEGEDDENKIIHRDSNGVILATGDSVVLIKDLKVKGSSMVAKQGTAVRNIRLD
                     HENAEYIEGRVDGQTIVIITQYVKKI"
     gene            complement(6089..7435)
                     /locus_tag="PLQ70_04895"
     CDS             complement(6089..7435)
                     /locus_tag="PLQ70_04895"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQE33986.1"
                     /translation="MNKKHHSNNILFNLGIENLNEMQVAAQETILSDNNVLLLSPTGS
                     GKTLAFLLPIFEMLKPEILSVQCLILVPSRELGLQIEQVWKKMGTDYKVNVCYGGHSI
                     ETEIKNLSNPPAVLIGTPGRIADHIDRGTFRLDKIETLILDEFDKSLQLGFHEQMSFI
                     IGKLSKLNKRVLVSATSDIEIPKYTRVVNPTILDFIPNEEETSNLATKMVISKEKDKM
                     GSLFNLIRSLKSQSAIVFCNHRDAAERISDTLNEKGIYSVYYHGGMDQDERERALIQF
                     RNGSVSYLITTDLAARGLDIPEMKHVIHYHLPLKEDEFTHRNGRTARMQASGTAYLIV
                     NESEKKLDYIDYGMEVLEVKNNTTLPKPPEFQTIYISGGKKNKLNKIDVVGFFSQKGK
                     LEKGDLGLIEVKDFISFAAVKFNKVKDLLHNIKDEKMKGKKFKIEVARKVIKKEEE"
     gene            complement(7544..8149)
                     /locus_tag="PLQ70_04900"
     CDS             complement(7544..8149)
                     /locus_tag="PLQ70_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQE33987.1"
                     /translation="MYKVFVNDKPLFLTNEISKETNFQLFLLESVDIVQLIVKIFQNK
                     IQKAYLYYPDEKEIMKTLKSKIPVSKAGGGLVYNKKGEVLFIFRGGKWDLPKGGTEKG
                     EEIEKTAMREVEEETGVNRLTITKKLQKTYHVFKRNGVYKLKITQWFEMQSDFDGIPV
                     GQIEEGIEKAVWIKPNEIPEVLKKSYENIKLLFEEEKISVQ"
     gene            8157..8804
                     /gene="pyrE"
                     /locus_tag="PLQ70_04905"
     CDS             8157..8804
                     /gene="pyrE"
                     /locus_tag="PLQ70_04905"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQE33988.1"
                     /translation="MIFNKDTAEKTAELLLQINAIKLNPRNPFTWASGWQSPIYCDNR
                     IILSFPTIRNYVRDEFSKNIEKQFGKPDVIAGVATGAIGIGMLVAESLGLPFVYVRPE
                     PKKHGRQNQVEGFLQSGQNVVIVEDLISTGNSSLLAVEALRAAGANVKGMAAIFTYGF
                     DVAQENFKNANVDLFTLSNYQNLLNLAVAKRYITEEEEATLREWNLSPSTWGVEK"
     gene            8870..9262
                     /locus_tag="PLQ70_04910"
     CDS             8870..9262
                     /locus_tag="PLQ70_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQE33989.1"
                     /translation="MNLESPKVTVEKSAEYLYTQLSDVRNFEKLMPENIAKFEVLGDD
                     IFNFGLKGMPEIKLRLKEGVPYSKIILNAASDKLPFTLVANINAISEYSSDVKLDFEG
                     EFNPMMAMMIKGPISKFIETLAGNMSKL"
     gene            complement(9341..10069)
                     /locus_tag="PLQ70_04915"
     CDS             complement(9341..10069)
                     /locus_tag="PLQ70_04915"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQE33990.1"
                     /translation="MKLIKLDAIDSTNEFLKGLSSNQSLENFTVVTAENQTKGKGQMG
                     AVWESEPSKNLIMSVLISDFVTDINQIFDINIAVSLAVIQVFEDFNIPELSIKWPNDI
                     MSYNKKIGGILIENSIKNGGNINSIVGLGLNVNQMNFENLPKASSLAVICNTTFDKEE
                     ILLEIIESLKQNIKSWNQKSDLLWSEYSNKLYKKGIPMPFEDQNKQNFMGIIQGVTSI
                     GKLEILLENDAIAEFGIKEIQMLY"
     gene            10165..>10465
                     /gene="rsfS"
                     /locus_tag="PLQ70_04920"
     CDS             10165..>10465
                     /gene="rsfS"
                     /locus_tag="PLQ70_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQE33991.1"
                     /translation="MTKKTINNDVLLANIIKGIEEVKGNDIDILDLREIDTAVCDYFV
                     ICNGNSNTQVNAIVNSIQKVVSKELKDKPWHVEGSDNAEWVLMDYVSIVIHVFHKH"
BASE COUNT         3472 a         1875 c         1752 g         3366 t
ORIGIN      
        1 actttgttga aagaaaattt gacacaatac aaacaaaaaa ataaaaaaaa agcatcaaaa
       61 aaaaatgaaa aaatctcttc ttgccattgc aatagcaatt ttactaactt cttgcagtcc
      121 aactttgaaa gtaaaacaat tggatgccaa aactggaaaa ctgcctacag aaactactct
      181 tactcaatct gaaatcttag tcaaaaagaa tataaaccta aaagaataca atcagttttt
      241 atttgtaaag aaaagcggtt taaatatgga aaaatatgaa acctatattt taggcacttt
      301 aaaaaatatt ggaggttttc aaaaatatta tatccaaact gaactagaac aatatgtgat
      361 tcagaatggt ttaacagata aagtaaccag catttccgac aatattggat tgctcaattt
      421 aagcaaaaat gtaggtaaat ttctaatttg cgaaagcaat gtggaataca aagggggtta
      481 cgatttcagt tttgaataca aagtaatcaa tcctacaaat gctgaaattt tacttcacat
      541 caaacacaat gctttcaatt ggggaggatt agacagaccg ctttttaatc ctgtattcaa
      601 cgaatatatt gattggttaa aagaaaatca aaatcaacaa taataattct caataaataa
      661 aagccctttc aacaattctg aaagggcttt tattctataa aaaattccgt ttatccgcca
      721 aaatcagtgt catcagcgtc ccaaacctta ctcctcctcc tcgctagcat gcgtttttga
      781 atagcttctc catttctcga gacaatcctg aaaatcctgt ggtaattcgg tatcaaaacg
      841 catatattct cctgttgtag ggtgcataaa accaagtgtt ttagcgtgta atgcttgacg
      901 aggcaatgcc ttgaaacagt tctctataaa ttgcttgtat ttggtaaacg tagttccttt
      961 taaaattaaa tcacctccat aacgggcatc gttgaaaagt gggtgaccaa tgtgtttcat
     1021 atgaacccga atttggtgtg ttcttcctgt ttcgagaatg caagaaacca aagtcacata
     1081 cccaaaacgc tctaaaacct tgtaatgcgt cacagctggt ttcccaattt cgggatcggc
     1141 aaaaacagcc atttgcatac ggtcttttag gtgtcgggca atgttgcctt caatcgttcc
     1201 ttcgttagca attacatttc cccaaactag cgcgatgtat tccctttcgg tggttttggc
     1261 ttcaaattgc ttggcgagat gtgtcattgc ggcttcggtt ttggcaataa ccaaaagccc
     1321 cgaagtgtct ttgtcaattc ggtgaaccag tcctgggcgt tcgctgctgt tcattggcaa
     1381 attctcaaaa tgaaatgcca aagcattcac caaagttcct gtataattgc cgtgaccagg
     1441 atgcactaca aaatcgggcg gtttattgat cagtaaaaca gcatcatcct catagacaat
     1501 gttcaaagga atatcttcgg gatcaaccct attttcaaaa ggcggatgcg acaacattat
     1561 tcgaatcaca tcaaagggtt ttaccttata attcgatttt acgggaatat catttacata
     1621 aatatcacca cccgtagctg cattttgaat tttatttcga gtagcattcg gaatcatatt
     1681 cattaaatat ttgtcaatcc tcaaaaactc ttgtccttta ggaacgtcaa atctgtaatg
     1741 ttcaaataat tcgtcttcta aatctacaat ttcttcgtta ttttcgctca ttgaatggaa
     1801 tctttttgca cagccaagct atccacttct tctacataac tttcttttcc gtcgcccaaa
     1861 accaaatcaa ttttagaagc tttcaaaact cggtcgcctg ctttaacatt tcttcctttg
     1921 taacgcattt ccaacaccat atcctttcca agattcggaa tataggtaat tgttccttct
     1981 tcaagtccca aagctttcaa agtgggaacc gcttcacgat aggttttgtc aatcaaatca
     2041 ggaattttca cagacgaaaa ccccgaagcg ttaattttta tatatacttt tctaccttct
     2101 tttacttttg ctcccggcaa cggatcttgc tctaccacac tgtattttgg ataatcacta
     2161 ttataatcca cactgtccaa aagcacataa tctaagtcca actcgtctag cttttcttcc
     2221 acttgttcct cggtcaattt agccaaattg ggcaccgtga tttcgtgacc atgatcggtg
     2281 gtaaaagtca accaatgcat aaacaaataa ctcaaaacgg caagaatagc taaagctgca
     2341 attgcttgtc caaaaaacac acgactggta agatacttac gtaagctcat aaatttattt
     2401 ttataagtcg caaagataaa gctatttcgt tccaaaaaaa attctaattt tgttaatagt
     2461 tttttaagga gctatttccc gctatccgtt acaaacgaag ttcaatgaac tccgcttaaa
     2521 ataaaagttt agtgaattaa tcttttgtgc cggtgttcgg cacaaaagat taattcacta
     2581 ctatcggggc tagggcatcc tacaatcaaa actatttatt tcaaactaaa acctaatacc
     2641 cagaacccaa tacctaaaac atgaaaaaca ttgccatcat aatgggcgga tattcaagcg
     2701 aatataaaat atccttaatc agcggaaacg ttgtctatca atttcttgac aaaaccaaat
     2761 acaacggatt tagaattcat attttcaaag aaaaatgggt ttatgttgac gataatgaaa
     2821 ctgaattccc tattgacaaa aacgattttt cgactaccgt aaacggaact aaaattactt
     2881 tcgactgtgt tttcaacgcc attcacggaa ctccgggcga agacggattg atgcaagcct
     2941 attttgaact aattggaatg ccgcaatctt catgcgatta ttatcaagcg gcattaacgt
     3001 tcaacaaacg cgatatgctt tcggttttaa aaccatacgg aatcaaaaca gctacttctt
     3061 attatttgaa caaaggagaa cacatcgata ctgccgaaat cgtaaaaacc gttggcttac
     3121 cttgttttgt aaaaccaaat aaatcagggt ccagctttgg aatttcgaaa gtaaaaaccg
     3181 aagccgaatt accaattgct attgaagtgg cttatcaaga ggacaacgaa attattattg
     3241 aaagtttctt ggatggtacc gaagtttctg ttggagtaat caattatcaa ggaaaagtaa
     3301 ttgttttgcc aatcaccgaa attgtttctg aaaatgattt ctttgattat gaagccaaat
     3361 accaaggaaa atctcaggaa atcacacctg ccagaatctc cgaagaaatg acccaaaaag
     3421 tgagtgaagc agccaaacgt gcttatgaag ttttgaaaat gaaaggtttc tcccgaagcg
     3481 aattcatctt cgtagatggc gaacctcata tgctcgaaat gaacaccatt ccgggattga
     3541 caaccgaaag tttgattccg caacaagcca aagcagcagg aatttcattg acggatttgt
     3601 ttacgaatgc tattgaattg gctttataaa aaagcaacta agaagctaag taactgaggg
     3661 acaaagtttt ttttttcgaa taaacgcctc agtttctgat attcttagta acttagagcc
     3721 ttagaaacta accacttaaa aatgattgac tataaacaat atatcgcaat aaatactcaa
     3781 attcgttttg ggaaacctac gattattggt actagaataa ccgtatttga tattttatat
     3841 tggatggcaa acggaatgag cataaatgat attattgaag attttccaga actaaatgaa
     3901 aaccaaatca aagcttgtct tgcctattct gccgatagag aacataaaat tcaaattaca
     3961 ttatgaaatt gttattggat caaaacatct ctcttagagt tatttctaaa attaaaaata
     4021 attttcccga ggcaaaacaa gtaaaagaac ttggaatcga aaattattta gatattgaaa
     4081 tatgggaatt tgccagagat aataagttta caattgtgac ttttgatgca gacttttatg
     4141 atttatctca tttaaatgga catcctccaa agataatttg gttacggttt ggcaatacta
     4201 caaatgattt aattgcttca atcataaact ccagatattc agttataaat gattttatta
     4261 acgccaaaga ctattcagaa attgtttgtt taaaaataaa ataaaaagat taaaatatga
     4321 acaaaagcag actcgaagcc tttagcgatg gtgtattagc cataatcatt acgataatga
     4381 ttttagaaat aaaagcaccc gaagacgata gtttcgaatc cttgcaacac ctgattcctg
     4441 ttttcttgag ttatgttttg agttttgtct atgttggaat ttattggaat aatcaccatc
     4501 atatgtttca ggtggtcaaa aaagtgagtg gttctatttt gtggagcaat ctgtttttgc
     4561 ttttttggct ttccttgatt ccttttgcca cgagttggat tggagaacat caattttcgg
     4621 ccgtacctat gagtgtttac ggctttgttt tgttgatgtg tgccgtttct tatatcattc
     4681 ttcaaaataa aatcatcaaa ctcgaaggca atgattcggt tttgcatcgt gcagtcaaca
     4741 gtgacactaa aggcaaaata tcgatggctt gctatatttc ggcaatccca ttggctttcg
     4801 tttcaccttg gatttctgga ctactatata ttgcagtagc cctaatttgg gtaattccag
     4861 attcaagaat tgaaaaacaa ttaaataaac tttaaaatga gaaaagctat ttttccaggt
     4921 tcgttcgatc caattacgtt aggtcacgaa gatattatcc gaagaggaat tactctattt
     4981 gacgaaattg taattgccat tggtgtcaac gccgaaaaaa aatatatgtt tacccttgaa
     5041 gaaagaaaac acttcctcga agatactttc aaagacgaac ccaaaatttc catcatcact
     5101 tatgaaggtt tgacaattga tttatgcaaa aaaataggtg ctaatttcat ccttcgtgga
     5161 ctaagaaatc cagccgattt tgagttcgaa aaagccattg cccacgccaa cagaagaatg
     5221 tctaaaattg aaaccgtgtt tctattgacc gctgcaaaaa catctttcat tagttcgagt
     5281 atcgtcagag atgtgattcg acataaaggc caatatgaat tattggttcc cgatgcagtg
     5341 agagtaaaaa aatagtgttt cctccttaaa tttgtaaaag gctttaatta acactacttt
     5401 cgcaaaaaat tagacaaaaa cagaaatgga aagaacatta aataaacgca gcggatcaca
     5461 atgtgaactt tgtgcagcaa ccgaaaactt aaaagtatat accgttttac caacaaaaaa
     5521 aggaggcatt gacgaaaata ttttggcttg ccccacttgt atcgaccaaa tagaaaatcc
     5581 tggacacgaa gaccaaaatc attggcgctg tttaaacgaa agtatgtgga gcgaacatat
     5641 tccggtgcaa gtagtttcgt ggcgtatgtt gagccgttta cgcaataatg aattggtaga
     5701 acaaatgtat cttgacgaag atactttagt ttgggcacaa gccactggtg aaggcgaaga
     5761 cgacgaaaac aaaatcattc accgcgatag caacggagtg attcttgcta ctggagattc
     5821 agtagtttta atcaaagatt tgaaagtaaa aggttcgagt atggtggcca aacaaggaac
     5881 tgccgtaaga aacataagac ttgaccacga aaatgccgaa tatattgaag gtcgtgttga
     5941 tggacaaacg attgtaatta ttacgcaata cgtgaagaaa atataattgg aagtgggaag
     6001 ttagaagtac caaaaaaaac gaaaacgtct tgataaatca agacgttttc gttttttata
     6061 ggaatgctta accttcttaa tctgaaatct actcctcttc tttcttgata acttttctcg
     6121 ccacctcaat tttgaatttc ttgcctttca ttttttcgtc cttaatgttg tgaagtaaat
     6181 cttttacttt attgaatttt acggcggcga atgaaataaa atccttgact tcaattaatc
     6241 ccaagtcgcc tttttcgagt ttcccttttt gggaaaagaa acctacaaca tcaattttgt
     6301 tcaatttatt tttctttcca ccactgatgt agatggtttg aaattcgggt ggttttggta
     6361 aagttgtatt gttttttact tccaaaactt ccattccata atcgatataa tcgagttttt
     6421 tctcgctttc gtttactata agataagccg ttccagaagc ttgcatacga gctgttcgac
     6481 catttctatg cgtgaattcg tcttccttta atggcaaatg ataatggata acgtgtttca
     6541 tttcaggaat atccaatcct ctcgcagcta aatccgtcgt tattaaataa ctcacgcttc
     6601 cgtttcggaa ttggattaaa gcgcgttcac gttcatcttg atccattccg ccgtgataat
     6661 aaacagagta aattcctttt tcgtttaagg tgtcacttat tctttcggca gcgtcacgat
     6721 gattgcaaaa aacaatggct gattgcgatt tcagggaacg aatcagattg aacaaacttc
     6781 ccattttgtc tttttctttc gaaatcacca ttttggtggc aagattactc gtttcttctt
     6841 cattaggaat aaaatccaaa attgtaggat tgaccactcg ggtatatttt ggaatttcaa
     6901 tatcggaagt ggccgaaact aaaacccgtt tgttcaattt ggaaagtttt ccaataatga
     6961 aagacatttg ttcgtgaaaa cccaactgca acgacttatc aaactcatcc agaatcaaag
     7021 tttctatttt gtctaaacga aaagttcctc tatcaatatg atcggcgatt cttcctggag
     7081 ttccaattaa aactgctgga ggattgctca aattcttaat ttctgtttca atggaatgtc
     7141 cgccgtagca aacatttact ttgtaatcgg ttcccatttt tttccaaact tgctcaattt
     7201 gcaaacccaa ttcacgcgaa ggaacgagaa tcaagcattg aacagaaaga atttcgggtt
     7261 taagcatttc gaaaatgggc aacaaaaaag ccaaagtttt cccggaacct gttggcgaaa
     7321 gcagtaaaac attattgtcg ctcaaaatgg tttcctgggc ggcaacctgc atttcattta
     7381 ggttttcgat tcctaaattg aacaatatat tgttggaatg gtgtttttta ttcattttgc
     7441 aaaggtagta gaaaagtgtt cagtaatcag tttttagtgt tcagatatga aaactgaaat
     7501 agaaaatgga atgactactg aacactgatt actgatcact tgattactga acactaatct
     7561 tctcttcttc gaacaataat ttaatgttct cgtacgattt ttttaagacc tctggaatct
     7621 catttggttt aatccaaact gctttttcga ttccttcttc aatttgtccc actggaattc
     7681 catcaaaatc tgactgcatt tcaaaccatt gtgttatttt tagtttataa acaccgtttc
     7741 gtttgaaaac gtgataggtt ttttgaagct ttttggtaat cgtcaatcga ttaactcctg
     7801 tttcctcttc gacttcccgc attgcggttt tttcaatttc ttcccctttt tctgttccgc
     7861 ctttgggtaa atcccatttt ccacctctaa aaataaataa gacctcgcct tttttattat
     7921 aaaccaatcc tcctcctgct ttagaaacag gaattttgga tttcaaagtt ttcataattt
     7981 ccttttcatc aggataatag aggtaggctt tttgaatttt attttgaaat attttaacta
     8041 taagttgcac tatatcaaca ctttctaata aaaataactg aaaattcgtc tcttttgaga
     8101 tttcatttgt caaaaaaagt ggtttgtcgt tcacaaaaac tttatacatt tgtactatga
     8161 tatttaataa agatactgcc gaaaaaactg ccgaattact tttgcaaatt aatgcaatta
     8221 aattgaatcc aagaaatcct tttacatggg cttcgggatg gcaatcacca atttattgtg
     8281 ataaccgaat aattctctca tttccaacta ttaggaatta tgttcgagac gaattttcaa
     8341 aaaacatcga aaaacaattt ggaaaacctg atgtaattgc gggtgttgcc actggagcca
     8401 ttggaattgg catgctagtt gccgaaagct taggattgcc gtttgtttat gttcgtccag
     8461 aacccaaaaa acatggccgt caaaaccaag tggaaggctt cctacaaagt ggtcaaaatg
     8521 tagtaattgt agaagattta attagtacag gaaacagtag tttgttggct gtagaagctt
     8581 tgcgagcagc aggagccaat gtaaaaggga tggctgccat atttacttat ggttttgatg
     8641 ttgctcaaga aaactttaaa aacgccaatg tcgatttgtt tactcttagt aattatcaaa
     8701 atctattgaa tttggcagtt gcaaaaagat acattactga agaagaagaa gctactctaa
     8761 gagaatggaa tttgagtcct tcgacttggg gagttgaaaa ataaattgat ttaatttagc
     8821 attgttgatt gtatcccaaa acttcgggat aaatataaat ttttagaaaa tgaacttaga
     8881 aagtcccaaa gtcacagtag aaaaatcagc tgagtattta tacactcaat tgtcagatgt
     8941 tagaaatttc gaaaaattaa tgcccgaaaa cattgctaaa ttcgaagttc ttggtgatga
     9001 tatttttaat tttggattaa aaggcatgcc tgaaattaaa ctaagattaa aagaaggtgt
     9061 tccttattct aaaataattt tgaatgccgc cagcgacaaa ttaccgttta ctttggtagc
     9121 aaatattaat gcaatttcag agtattcgag cgatgtaaaa ctagatttcg aaggcgaatt
     9181 caatccgatg atggctatga tgatcaaagg acctatttcg aaatttattg aaactttggc
     9241 tggaaacatg tcgaaattgt aaaaattaga taattcacaa accaaagacc ttttagttca
     9301 aactgagagg ttttttttat tataaatagg ttcatattat tcaatacaac atctgaattt
     9361 ctttaatacc aaattcagct atagcatcat tttccaaaag aatttcaagt tttccaatgg
     9421 aagtaactcc ttgaataatt cccataaaat tttgtttatt ttgatcttcg aatggcatcg
     9481 gaattccttt tttgtagagt ttattactat attctgacca taataaatcg gatttttgat
     9541 tccaactctt gatgttttgt tttaaacttt cgataatttc caaaaggatt tcttctttat
     9601 caaaagtcgt attacaaatt accgccaagg aagatgcttt aggcaaattt tcaaaattca
     9661 tttggttgac atttaagccc aaacccacta ttgaattaat atttccgcca ttttttatac
     9721 tattttcgat taaaattcca ccaattttct tgttgtatga cataatgtcg tttggccatt
     9781 tgatgcttaa ttctggaata ttaaaatctt caaatacttg aattactgct aatgatactg
     9841 caatattaat atcaaatatc tgattaatat ctgtaacaaa atcagaaatc aaaacactca
     9901 ttataaggtt tttactcggt tcagactccc aaacagctcc catttgcccc ttgcctttgg
     9961 tttgattttc ggcagtaaca accgtaaaat tttcaagtga ttgattgctc gataaccctt
    10021 ttaagaactc atttgtagaa tctatggcat cgagtttgat tagtttcata ggaagttatt
    10081 tgtggaacag aatttaatat tatgttaagg ttcaaaaata atcacaaaaa atggtaactt
    10141 tacaaactta tttaaaaata atacatgaca aaaaagacaa taaataatga tgttctattg
    10201 gcaaacatca tcaaagggat tgaagaagta aaaggaaatg atatcgatat tctggattta
    10261 agagagatag acaccgccgt ttgtgactat tttgtaatct gcaacggaaa ttcaaacact
    10321 caggttaacg ctattgttaa ctccattcaa aaagtagttt caaaagaatt gaaagacaaa
    10381 ccttggcatg tagaaggatc agacaatgcc gaatgggttc ttatggacta tgttagcatc
    10441 gtgatacacg ttttccataa acaca
//
LOCUS       DAOSNN010000065        10427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6391_length_10427_cov_5.039433, whole genome shotgun sequence.
ACCESSION   DAOSNN010000065 DAOSNN010000000
VERSION     DAOSNN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10427
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6391_length_10427_cov_5.039433"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLQ70_04925"
     CDS             <1..318
                     /locus_tag="PLQ70_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQE33992.1"
                     /translation="YDFAPSGYYILSDSAEILGLNLMGANILGKERSYLLHRRFTFFI
                     DDDDKIAFSSFLNKVFASETKVSCEIRLYINKTTLYLHLDGIAIENKKQSLITALDIT
                     EKK"
     gene            594..1658
                     /locus_tag="PLQ70_04930"
     CDS             594..1658
                     /locus_tag="PLQ70_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQE33993.1"
                     /translation="MMPIPTNKKFSVLEILQYTVLISILLYFGKPLFIPLSFALLISF
                     ILYPVCKWMEKKGISKSISIFVSIFGILLFFGALLYLLFLQLIEFSNEWQLLKVKLLE
                     TGKQWSIYLSEQFNLSSEKQSLFLKNALNNSGAQTILFIQNTLSSLSESVFYLLIIPI
                     YSALILYYRQLLVDVLCHIFSSEKKETIREILIQTIHSYYDFIKGMLVVYLLVGLLNS
                     IGLALIGVPHPFLFGFIASILTFIPYVGILISSLLPITISWITFNSIWYPIGVIAVFS
                     LVQLIEAYIIFPLAVGKRLKINTLVIIVVIIIGGILWGAAGMILFIPFVSIAKLIAEK
                     TNGLKTLAILLGEGESKKRD"
     gene            complement(1655..2557)
                     /locus_tag="PLQ70_04935"
     CDS             complement(1655..2557)
                     /locus_tag="PLQ70_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HQE33994.1"
                     /translation="MKFTKPSYLKKGDTIMIVAPAGFVPDSTEIEPGIELAKSWGLEV
                     IVGKNAFKKHNHFAGTDEERQTDLQLALDDKKIKAIWCSRGGYGTVRIIDQLDFTAFE
                     KNPKWVIGFSDITTLHTTIHNLGIATIHATMPGGIKRASDEAKQTLYKALFGYSYGLE
                     IASNSLNKKGSAKGVLIGGNLSIVNSMIGSVSEVNTYDKILFIEDVGEDLYRVDRMMY
                     TLKRTGALKNLKALIVGDFDYNIEKDTLFGGTHREIILNAVNEYNYPVIFDFPAGHIR
                     DNRALIFGKEITIEVNETVSKIWH"
     gene            complement(2739..3782)
                     /locus_tag="PLQ70_04940"
     CDS             complement(2739..3782)
                     /locus_tag="PLQ70_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HQE33995.1"
                     /translation="MKATYHKYILNFKRPSGTSRGVMTEKETWFIVLEKDGKKGIGEC
                     GILRGLSADDRPDYETKLQWTCDNIHLGEKQLWEALIEFPSIQFGVEMAFQSLASETP
                     FLLFSSDFTKGEKSIEINGLVWMGEESFMKQQIEEKLADGFRCVKLKIGAIDFDKELQ
                     LLRYIRQYFTPEQVEIRVDANGAFDVTLALYKLNQLSGFKLHSIEQPIQKNNTDRMSE
                     LCEITPIPIALDEELIGVFSLEEKEALLQKIKPQYIILKPSFVGGFRGAKEWILLAEK
                     YNIGWWITSALESNIGLNAIAQWTFLQHNLMPQGLGTGALYTNNFDCPLQVSEGQLWY
                     KKELNWKFDFEFF"
     gene            complement(3846..4796)
                     /locus_tag="PLQ70_04945"
     CDS             complement(3846..4796)
                     /locus_tag="PLQ70_04945"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HQE33996.1"
                     /translation="MSFTLLSSPLQGFTDFRFRNAQNKLFGGIDTFYSPYIRLNGKLE
                     IKPSYQRDLLPENNLDLEVIPQVITNNAEEFLFVAKYVRELGYKELNWNLGCPYPMVT
                     KSGMGSGLIKNTQQINHILDRAHSETDIIVSMKMRLGYENTEEILDVLPILDRYPIKN
                     IAIHARIGKQLYKGGVHLDAFQHCIDNTKHKLYYNGDITSVAKFHEMQERFPTIDHWM
                     IGRGLISDPFLPSMIRNNSVEYPENKMELFSEFHDTLYAIYSESLSGQSHILLKMYHL
                     WEYFSVTFSNPHKVLKQIKKAQSIRNYEAAVASIFKNEKI"
     gene            4918..5784
                     /locus_tag="PLQ70_04950"
     CDS             4918..5784
                     /locus_tag="PLQ70_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQE33997.1"
                     /translation="MKNFTDFKTYNEYLGLNQPLDNDIDVGYYDPPNMLSTSEPITVD
                     FYRISIKINVVNKTIPKSDPITAVFFNSPNLIVPTGWDVEPTYSGMYLQLSKKIIEEN
                     RYLFKTYLDYGQHEALYLKEDEVQEISAVFRLMMKYYESKKQNYNVLLSYVNVLVSLV
                     EAFYKRQFSTDPKEYNSIVTDFQQSLIDYYNQPVSQIPSVQYFADKLGLTANYLGDIV
                     KHFTQKSALENIHDFVIKRAKELLVENKKLNTAEVAYELGFEYPNYFSKFFKKQVNLT
                     PKEYRLQAIGKN"
     gene            5919..6524
                     /locus_tag="PLQ70_04955"
     CDS             5919..6524
                     /locus_tag="PLQ70_04955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQE33998.1"
                     /translation="MIKSKKVLLINTHLTYPNWTEGTLNNSFIQKAKDFFGTKGIEVL
                     ETKVEAGYNANEEVEKHMEADIIILQTPVNWFGGPWIYKKYVDEVFNSGLQSTKFLSG
                     DGRTREDATKQYGTGGKMQGKKFMVCATWNAPSEAFGNPSQILMQGKGTSDLFLPITS
                     NYRFCGAEIVSDYNCFDIFKDGNIAEVLENYPTHLEKVFEL"
     gene            complement(6819..7316)
                     /locus_tag="PLQ70_04960"
     CDS             complement(6819..7316)
                     /locus_tag="PLQ70_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HQE33999.1"
                     /translation="MKIQIRVYQPEDTQAILDIINHNILHSTALYDYNIRSYEQQQTI
                     LEEKINKNYPVIIAELNGQVVGFGMYSEFRFREAYKFTVEHSVYVNKDFHGKGIGKLL
                     LQELISLAKKQKLHTMIAVIDAENQSSVEFHEKFGFKTVGIIKESGYKFDRWLDSVFM
                     QLILE"
     gene            7497..9158
                     /gene="menD"
                     /locus_tag="PLQ70_04965"
     CDS             7497..9158
                     /gene="menD"
                     /locus_tag="PLQ70_04965"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HQE34000.1"
                     /translation="MIYPKIPLAQSIIQICLDKGIKNIVISPGSRNAPLTIGFVNNPE
                     FNCYSIADERCAAFFALGIAQQIQKPVAVVCTSGSALLNYYPAFAEAFYSEIPLIVIS
                     ADRPQSKIDIGDGQTIRQENVFQNHSLYNANLTEEVSVENDLKINEAINEAFAKKGPV
                     HINAPFEEPLYQTVSKLNVDVTLSTFGKKNNKVSIDDIDEFTSIWNQFEKKLILVGEN
                     KPNTIDTTIIEALANDPSVVVMTETTSNIHHPSFLNNIDTIITPFSQKDFENLQPNIL
                     ITFGGMVVSKRIKAFLRQYKPTHHWHIDTLRAYDTYDCLTKHFEVDPNQFFTQFLPFT
                     IPIASNYFSTFNAISALREKKHQEYLAKTPFSDFKAFEKIIPSLPDNSMLHISNSSAI
                     RYAQLFSIKPSITVFCNRGTSGIDGSTSTAIGAAVANERQTTLIAGDIGFLYDSNALW
                     NEYTPKNFKIILINNGGGGIFRILPGHEETPVFNKFFETSHCLTAEQLAKMYGFEYSI
                     ASDEVSLEKNLIALYSQNDKPAILEIFTPTLENNKILLQYFKELA"
     gene            9342..>10427
                     /locus_tag="PLQ70_04970"
     CDS             9342..>10427
                     /locus_tag="PLQ70_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34001.1"
                     /translation="MLETYIRNKYCSWILSFWTLFSIAFLQAQDTKSIPDIEKVYLHT
                     DRNLYFIGEDLWHKAYNVNAYTNLLYDNSTILYVELVAPDSKIVARNKTNLEIGIGNG
                     DFQLKDSLGIKPGHYQLRAYTNWNRNFGDNFVFKKEIEIINVFEDHSKFNKTQNGTTI
                     INPKTIEANKQNTFKVDFFPEGGSLLENVVGVVGFKAIDSNGKPIEIKGELFDPNNEL
                     VTAFESVHDGMGKFQLVPIEEKKYFAKIQNPAGEEIKVELPSVLKQGYSLHFRTIKGR
                     NIVNITTNQATLSQNPNAQLTVVCKTKGIPYFETTKTLTETSLSFELPKDKTPDGISQ
                     ITLFDNTNKPQSERLIYIEKNQDLEVQL"
BASE COUNT         3539 a         1815 c         1656 g         3417 t
ORIGIN      
        1 tatgattttg cgccttcagg ctactacatt ctatctgatt cagccgaaat tctaggatta
       61 aacttaatgg gagccaatat tctaggcaaa gaacgcagct atttattaca ccgacgattt
      121 acctttttta ttgatgatga tgataaaatt gcttttagct cctttctcaa caaagttttt
      181 gcaagcgaaa ccaaagtaag ctgtgaaata agattatata taaacaaaac tactctttat
      241 cttcatcttg atggtattgc catcgaaaat aaaaagcaat cgcttataac tgcacttgat
      301 atcacagaaa aaaaataaat gaacaaagac taaaaataaa tgaacaaaaa taccaaacta
      361 tttttgaaaa tgaaagagac agtaataggt atatttagac ttgacaaaga tggggaaccc
      421 ggtcgtttta ttgaagtaaa taatgctact acaagctttg agtgcgttaa taaacaagca
      481 tttcaaataa tttgattaat ttttatataa aaaactaaaa cggtaattca aataaaatga
      541 agatgaatta atcttttcaa aaagtttaaa aagcttataa tttctgaatt attatgatgc
      601 caatcccaac gaataagaaa ttttctgttt tagaaattct gcagtacact gtattgattt
      661 caatcttgct ttactttgga aaacctcttt ttattccgtt gagctttgcc ttactcatca
      721 gtttcatact ttatcccgta tgtaaatgga tggagaaaaa aggaattagc aaaagtattt
      781 ctatattcgt ttctattttt ggaatcttac tcttttttgg agccctactc tatttacttt
      841 ttttgcaact aattgaattt tcgaacgaat ggcaattatt aaaagtaaaa ctgttggaaa
      901 caggaaagca atggagtatt tatttgtcgg agcaattcaa tcttagctcc gaaaaacaat
      961 ctttatttct aaaaaatgca ctcaacaatt caggagcaca aaccatttta tttatacaaa
     1021 acactttatc ttctctatcc gaatcagtat tttatttact aattatccct atttattcgg
     1081 cgcttattct ctattatcgt caattattgg tcgatgtact ttgtcacatt ttctcatcag
     1141 aaaaaaaaga aaccattcga gaaatcctta ttcaaactat tcactcctat tatgacttta
     1201 tcaaaggaat gcttgtggtg tatctattag tcggactttt gaatagtatt ggacttgcat
     1261 taattggcgt tccgcatcca ttcttatttg ggtttattgc ttccatttta acttttattc
     1321 cttatgttgg aatcctaata tcatcgcttt tgcctatcac aatttcttgg attaccttca
     1381 attctatttg gtatccaatt ggagtaatag cagtttttag tttagttcaa ctaatcgaag
     1441 cttatattat ttttccgctg gcagttggaa agcgactaaa aataaataca ttagtcataa
     1501 ttgtagtaat cattattggc ggaatactat ggggagcagc gggaatgatt ttgtttattc
     1561 cgtttgtaag cattgcaaaa ttaattgcag aaaaaaccaa tggtctaaaa acattagcta
     1621 tccttttggg cgaaggagaa agtaaaaaac gtgattaatg ccaaattttt gaaaccgttt
     1681 cattcacctc aattgtgatt tcttttccaa aaatcaaagc tcgattgtca cgaatatgtc
     1741 cagctggaaa gtcaaaaatt acaggataat tatactcatt tacagcattt aatataattt
     1801 cacgatgggt tcctccaaat aaggtgtctt tttcaatgtt ataatcaaaa tcacctacga
     1861 taagggcttt caagtttttc aatgcaccag ttcttttcaa agtgtacatc attctatcta
     1921 cacgatacaa atcctctcca acatcttcaa taaacaaaat tttatcataa gtattcactt
     1981 cagaaacact tccaatcatc gaatttacta ttgataaatt tcctcctatc aaaactcctt
     2041 tggcacttcc tttcttattc aaagaatttg atgctatttc caatccataa ctataaccaa
     2101 atagagcttt atacagagtt tgcttggctt catcagaagc tcgctttatt ccgccaggca
     2161 tcgtagcgtg aatagtagca attcctagat tgtgaatagt ggtatgcaaa gtagtaatgt
     2221 cagaaaaacc aataacccat tttggatttt tttcgaaagc tgtaaaatcc aattggtcaa
     2281 taatgcgaac cgttccatat ccacctcttg aacaccaaat tgctttaatt tttttatcat
     2341 ccaatgccaa ttgcaaatcg gtttgacgct cttcatcggt tcctgcaaaa tgattgtgtt
     2401 ttttaaaagc attttttccg actattactt ccaatcccca gcttttggct aactcaattc
     2461 caggttcaat ttcggtagaa tcaggaacaa atcctgcggg agccacaatc ataattgtat
     2521 ctcctttttt aagataagaa ggttttgtga atttcataga ttgcaattta ttctgatctt
     2581 gtctaactaa aggatcttgt ttcatggttt gataatggga tttacattgt acaaaaacaa
     2641 ctataaaaag cagttgaatg caaattaaag gaactctttt ttgtgttcta ttttctcgtt
     2701 ttttcatata gaaatactgc tttatttttg atagaaatct aaaaaaattc aaaatcaaac
     2761 ttccaattga gttctttttt ataccacaat tgaccttccg aaacttgtaa agggcaatcg
     2821 aaattattgg tatataaagc tcctgttcca agtccttgtg gcattaaatt gtgctgcaaa
     2881 aaagtccatt gtgcaatagc atttaatcca atattcgact ccaaagccga ggtaatccac
     2941 caaccaatat tgtatttttc ggccaataaa atccactctt ttgcaccacg aaaaccaccc
     3001 acaaaactag gcttcaaaat gatatattgt ggcttgattt tttgcagtaa agcttctttt
     3061 tcttccaatg aaaacacacc aatcaactct tcgtctaaag caataggaat tggagtgatt
     3121 tcacatagct ctgacatcct gtcagtatta tttttttgaa taggttgttc tatactatgt
     3181 aatttaaaac cagataattg atttaattta tataaagcta aagttacatc aaaagctccg
     3241 ttagcatcaa ccctaatttc aacttgctct ggagtaaaat attgacgaat ataccgcaat
     3301 aattgtagtt ctttatcaaa atctatagct cctattttga gtttcacaca acgaaaacca
     3361 tcggctagtt tttcctcaat ttgttgcttc ataaaggact cctcgcccat ccaaaccaag
     3421 ccattaattt cgatgctttt ttctcctttt gtaaagtctg atgagaagag taaaaaaggc
     3481 gtttcactcg ccaaagattg gaatgccatt tccactccaa attgaatcga aggaaactcg
     3541 attaaagctt cccaaagttg tttttcgccc aaatgaatat tgtcacaagt ccattgcaat
     3601 ttggtttcat aatcaggtct atcgtcagcg cttaatcctc tcaaaattcc acactcacct
     3661 attccttttt ttccgtcctt ttccaaaaca ataaaccaag tttctttctc ggtcattacg
     3721 ccacgagaag ttcccgaagg tcgtttaaaa tttaaaatat atttgtgata ggttgctttc
     3781 aaaattagat atctggtttt atcaagttca actttgtctt cccttctggt tgacaattgt
     3841 aataattata ttttttcgtt tttaaaaatc gatgccacag ccgcctcata atttcgaata
     3901 ctctgtgctt ttttaatctg tttcagcact ttatgcgggt tcgaaaaggt caccgaaaaa
     3961 tattcccaaa gatgatacat cttcaacaaa atatggcttt gtccagacaa agattcacta
     4021 taaatagcat acaaagtatc gtgaaattcg ctaaacaatt ccattttgtt ttcaggatat
     4081 tcaactgaat tatttcttat catactgggc aaaaaaggat ctgaaatcaa tccacgtcca
     4141 atcatccaat ggtcaatagt tgggaatcgt tcttgcattt cgtgaaactt agctaccgaa
     4201 gttatatcac cattgtaata caatttgtgc ttggtattgt ctatacaatg ctgaaaagca
     4261 tccaaatgaa caccaccttt gtacaattgt ttgccaattc gagcgtgaat ggcaatattc
     4321 ttaatcggat atcgatctaa aataggcaaa acatccaaaa tttcttcagt attttcataa
     4381 cccaaacgca ttttcatcga tacgataata tccgtttcag aatgggctct gtccagaata
     4441 tgattgattt gttgtgtgtt tttaataagt ccagaaccca ttcctgattt ggttaccatt
     4501 ggatacgggc aacccaaatt ccagttcaat tctttataac ccaattcccg aacatatttg
     4561 gcaacaaata aaaattcctc ggcattatta gtaatcactt gcggaatcac ttctaaatcc
     4621 aaattatttt ctggcaataa atctctttga tatgacggtt ttatctccaa tttgccgttc
     4681 aaacgaatgt atggcgaata gaaagtatcg attccaccaa aaagtttgtt ttgggcattt
     4741 ctaaaacgaa aatcagtaaa tccttgtaaa ggagatgaaa gaagggtaaa gctcataaat
     4801 atttgtaaag aggcaaagat aagggattcg tttttagcaa tgggaatagg aatgttagta
     4861 tctactttac aaaaggaatg aaccattaaa aaaaagataa atttgtaact ttgtcaaatg
     4921 aaaaatttca cggactttaa aacgtataac gaatatcttg gattgaacca acctttggac
     4981 aatgatattg acgtagggta ttacgaccca ccaaatatgt tgtctacatc tgaaccgatt
     5041 acggttgact tttacagaat ttccataaaa ataaatgttg taaacaaaac aatacctaaa
     5101 agtgatccca tcactgcggt gttttttaat agtccgaatt taattgttcc gacaggatgg
     5161 gatgtagagc ccacttattc aggaatgtat ttgcaacttt caaagaaaat tatagaagaa
     5221 aatcggtatt tgttcaagac ttatctcgat tacgggcaac acgaagcctt gtatttgaaa
     5281 gaagatgaag tacaggaaat cagtgccgtt tttcgattga tgatgaagta ttacgaaagt
     5341 aaaaagcaaa actacaatgt gcttttgtct tatgtaaatg tgttggtttc tttggttgaa
     5401 gccttttaca aaagacaatt ttcaacagac ccaaaagagt ataacagtat cgtaaccgat
     5461 tttcaacaaa gtttgattga ttattacaat caacccgttt cacaaattcc aagcgttcag
     5521 tattttgccg acaaattagg gttgactgcc aattatttgg gcgacattgt aaaacatttt
     5581 acccaaaaat ccgcactcga aaatattcac gattttgtta ttaaaagagc aaaagaatta
     5641 ttggtagaaa acaaaaaact aaacactgca gaagtagctt acgaactggg ttttgagtat
     5701 cccaattact tttccaaatt ctttaaaaaa caggtgaacc tcacgccaaa agaataccgg
     5761 ttgcaagcaa tcggcaaaaa ttaatacaat gaaaacgtct tgtcaaaggc gttttttttt
     5821 gttataagta atttgtttct tttttagtag tagtctgttt cctttgttgg caatactagt
     5881 ttttactttt gtggagtaaa aattaaacaa atagacgaat gataaaatca aaaaaagtat
     5941 tgcttatcaa tacgcactta acctatccaa attggacgga aggaacatta aacaattcat
     6001 ttattcagaa agcaaaagat ttttttggaa caaaaggaat tgaagtttta gaaacaaaag
     6061 tagaagccgg ttacaatgca aacgaagagg tagaaaaaca tatggaagcc gacatcatca
     6121 ttttacaaac acctgtaaac tggtttggag ggccttggat ttacaaaaaa tacgtggacg
     6181 aagttttcaa cagcggattg caaagcacca aatttctttc aggagatggg cgaacaaggg
     6241 aagacgcaac gaaacaatac ggaacgggcg gaaaaatgca aggcaaaaag tttatggttt
     6301 gtgccacttg gaatgcgcca tcagaagcct ttggcaaccc atcgcaaata ctgatgcaag
     6361 gaaaaggaac gtccgattta tttttgccaa ttactagcaa ttatcgtttt tgtggagccg
     6421 aaattgtatc tgattataat tgtttcgata tttttaaaga cggaaatata gcggaagttc
     6481 ttgaaaatta tccaacccat ttggaaaaag tgtttgagtt gtaaataaaa aatcaaataa
     6541 gatttgacgc agagtttgcc atttaatctg taacccgata gcgaacacaa tgtcattaag
     6601 ttaaggattc gtcctcgtct gttcgagtgt tttattaaaa aatgcgatag ctttttttaa
     6661 taaaatgtat cgagaacttc tattacaaaa ggcttctcga tacaattttg aatagtaaag
     6721 ctgtcgcatc acaattcaaa atcactcaaa gtgacggtaa atccttaact taataacatt
     6781 gatagcgaac aggagaagca aatctgtttc aaaatttttt actccaaaat caattgcata
     6841 aaaaccgaat ctaaccaacg atcaaattta tagcccgatt ccttaataat tccaacggtt
     6901 ttgaaaccaa atttctcgtg aaattccaca ctactttgat tttcagcatc gataacagca
     6961 atcattgtat ggagtttttg ttttttggct aaagaaatca attcctgaag caataatttg
     7021 ccaatgcctt tgccgtgaaa atctttgttt acataaacgg aatgttccac cgtaaattta
     7081 taggcttccc gaaatcggaa ttcactgtac attccaaaac caactacttg cccattcaat
     7141 tcggcaataa ttacgggata gtttttattt attttttctt ctaaaatagt ttgttgttgc
     7201 tcataacttc ggatattgta atcatataaa gcagttgaat gcaaaatatt atggttgatg
     7261 atatccaaaa tggcttgggt atcttcaggt tgataaactc tgatttggat tttcatttgt
     7321 aaaaagattt catttcaaaa gtatgaaaaa gttgatttaa ttaaaggata tcggtaggca
     7381 agtttgtaac tttgctgaat tgaggtttca aacaacttta aacttttaaa cttttaaact
     7441 tttaaacttt taaactttta aacttttaaa ccttaaactt ttaaaccaaa actttaatga
     7501 tttatcctaa aatacccctt gcacaaagca tcatccagat ttgtttggac aaaggaatca
     7561 aaaatatagt aatttctcca ggctccagaa atgcgccttt aaccataggt tttgtaaaca
     7621 atcccgaatt taattgctac agtattgccg acgagcgttg cgctgctttt tttgcccttg
     7681 gaattgccca acaaattcag aaacccgttg ccgtagtttg tacttcgggt tcggcattgt
     7741 tgaattatta tcctgctttt gcggaagctt tttacagtga aattccatta atcgtgattt
     7801 cggcagatag gcctcaaagc aagattgaca ttggtgacgg tcaaactatt cgtcaggaaa
     7861 atgtgttcca aaatcattca ttatacaatg ccaatttaac cgaagaagtt tcggttgaaa
     7921 atgatttaaa aatcaacgaa gccattaatg aggctttcgc caaaaaaggc cctgttcata
     7981 tcaatgcgcc gtttgaagag cctttgtacc aaacggtttc gaaattgaat gtcgatgtga
     8041 cgctttcgac tttcggtaaa aaaaataaca aagtttcaat tgacgatatt gatgagttta
     8101 ctagtatttg gaaccaattc gaaaaaaaat taattcttgt tggtgaaaat aaacccaata
     8161 caatcgatac aacaattatt gaggctttag ccaacgatcc atcggtagtg gtgatgacag
     8221 aaacgacttc gaacatacat catccttcgt ttttgaataa tattgatact ataattactc
     8281 cattttcgca gaaagatttt gaaaatttac aacctaatat actaattact tttggcggaa
     8341 tggtggtttc caaaagaata aaagcctttt tgagacaata caaacccaca catcattggc
     8401 atattgatac tttgagagct tatgatactt acgattgctt gacgaaacat tttgaagttg
     8461 atcccaatca attttttact caatttttgc cttttacgat tcctattgca agtaattatt
     8521 tttcgacttt taatgccatt tcagctttaa gagaaaagaa acatcaggaa tatttagcca
     8581 aaactccctt ttcggatttc aaggctttcg aaaaaataat tccatccttg cccgataatt
     8641 cgatgttgca tataagtaat agttcagcca ttcgatatgc acaattgttc tccataaaac
     8701 cttcgattac tgttttctgt aatcgtggta ccagcggcat tgatggtagt acttctacgg
     8761 caattggtgc agctgttgcc aacgaaagac aaactacttt aattgcggga gacatcggct
     8821 tcttgtatga cagcaacgca ttgtggaacg aatacactcc caaaaacttc aaaatcatac
     8881 tcatcaacaa tggcggggga ggaattttta ggattttgcc cggacacgaa gaaacgccag
     8941 tatttaacaa atttttcgaa acttcgcatt gcctaacagc agaacaattg gcaaaaatgt
     9001 atggttttga atatagtatc gctagtgatg aagtaagttt agaaaagaat ttaattgctt
     9061 tgtattctca aaatgataaa cccgctattt tggaaatttt tacaccaaca ttagaaaaca
     9121 acaaaatctt gttgcagtat ttcaaagaat tagcatagat tatgaagaat aatgggctaa
     9181 ataaatagta ggttgattat gaaaagatac tacttattgt attgaaagta atcgatttaa
     9241 aatttgttgg tacggttgtt gctatatttt gttaatataa attaacaaga tttttatcct
     9301 cttccttttc tcattgttta atataaatat atagatttta aatgcttgaa acttatatca
     9361 gaaataaata ttgcagttgg attctttcgt tttggacact attttcaatt gcttttttgc
     9421 aggcgcaaga cacaaaatcg attcctgata ttgaaaaagt atatcttcac accgaccgaa
     9481 acctctattt tattggggaa gatttatggc acaaagctta taatgtgaat gcttacacca
     9541 acttacttta tgacaatagc actattttgt atgtagaatt agtggcaccc gattctaaaa
     9601 ttgttgctcg aaacaaaacc aatttagaaa taggtatagg caatggcgat tttcaattaa
     9661 aagattctct tggcataaaa ccaggacatt atcaattgag ggcttacacg aattggaatc
     9721 gaaattttgg ggataatttt gtgttcaaaa aagagattga aattatcaat gtttttgaag
     9781 atcattctaa atttaataaa actcagaatg gcacaacaat cattaacccc aaaaccattg
     9841 aagcaaataa acaaaacacc ttcaaagtgg attttttccc cgaaggtggt tcgcttttag
     9901 agaatgtagt tggtgttgtt ggttttaaag caattgatag taacggaaaa ccaattgaaa
     9961 ttaaaggcga actatttgat cctaataatg aattggtaac tgcatttgaa agtgttcatg
    10021 atggtatggg gaaatttcag ctcgtaccaa ttgaagagaa aaaatatttt gcaaaaattc
    10081 aaaatccagc aggagaagaa attaaagtgg aacttccaag tgttctcaaa caaggttatt
    10141 cactccattt tagaacaata aaagggcgga atattgtaaa tattacgact aatcaagcta
    10201 ctttatctca aaaccccaat gcacagctta cagtagtttg taaaacaaaa ggaatcccct
    10261 attttgaaac aacaaaaaca ctaacagaaa cttctttgtc ttttgaatta ccgaaagaca
    10321 aaactcccga tggcattagt caaatcacat tattcgacaa tacaaataaa ccgcaatctg
    10381 aacgattaat ttatatcgaa aaaaatcagg atttagaggt gcaacta
//
LOCUS       DAOSNN010000066        10392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6424_length_10392_cov_5.330754, whole genome shotgun sequence.
ACCESSION   DAOSNN010000066 DAOSNN010000000
VERSION     DAOSNN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6424_length_10392_cov_5.330754"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2824
                     /locus_tag="PLQ70_04975"
     CDS             <1..2824
                     /locus_tag="PLQ70_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HQE34002.1"
                     /translation="ATLFIALLFWLGLRWEQDMDSPKGNKWLLVISLVVGLTFGVHFM
                     ALLTIPAIGFLYYFKHYKEVTVKNFIIANIVVVAVLLFIFKLLLPLTMAFFGKTEVFM
                     VNELGMPFDSGTIFVVLLLIAFFYFSLKYTRAKGLIKYNTLILCILFILIGFSTWMML
                     PIRANANTVINENKPSDAREVLAYYNREQYGSNPLFYGPQYTEAFAGLDENNPYLDKA
                     PNYERDYKTGKYVIVNNFKNAEQNSDDNQKTFLPRLWSGENITSYISFTNPPEFKLNP
                     DYAYEQDLPKYGVDPNQLSEEDLAKATAQLRNEIEKTVVEFRKAYAQKQLDNDDYVKF
                     LRTYSDYLIVEKPSTFDNLRFMAEYQFGYMYFRYLMWNFVGRQNDIQGRYDYQDGNWL
                     SGITFIDELHLGTQQNLPSDVLNNKGRNVYFFLPLILGLIGIMYHASRDRKSFYVLLV
                     LFLFMGLALKIYLNERPFEPRERDYALVGSFYVFAIWIGFGVYSLYESIQKYLAPKIA
                     GPIIIGASLLAVPALMAFQNWDDHDRSGKYTATALAKAYLESCDPNAILFTIGDNDTF
                     PLWYAQEIEHIRTDVKIVNTSLFMTDWYIDQMKSKTYESNPLPISFNHDEYVGDKLDY
                     VAHIQKVESRWEIKDFINFIKNPKSTVEMQNGQTIHFYPTNKIRIPVDKNNIIQNKVV
                     DAKFNDSIVPYIDIDIKGNALYKNRLMMLDLVANNNWKRPIYFSPGAFDDEDYLWMKN
                     YLQLEGMIYKLVPLKTKVDKDTNPMDMGGLDADKMYSIVMKWDWGNSNGDIYHDPETR
                     KNSISYRSVLSRLMDKLIAEGKMDKAKNIINLSLTKMPVEKFGYYSMVEPFAKGYYQI
                     GEKEKAQQLLGKLIGKYNENLNYYGTLSSSDQVDLAIDIITDIERYRSLLHVMKESGD
                     IQLYNKSRPVFNTYIKMFERFGRDRE"
     gene            2906..>3220
                     /locus_tag="PLQ70_04980"
     CDS             2906..>3220
                     /locus_tag="PLQ70_04980"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQE34003.1"
                     /translation="MNFYWVKTNRFIKKLFSNYVWDIPNTENKIYLTFDDGPTPEITE
                     WVLEELEKHNAKATFFCIGNNIKKHPEIFEKLISKGHSIGNHTFNHLNGWKTSTDDYL
                     ENS"
     assembly_gap    3221..3320
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3321..3563
                     /locus_tag="PLQ70_04985"
     CDS             <3321..3563
                     /locus_tag="PLQ70_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQE34004.1"
                     /translation="SQAKKLKQLGYQIIMWDVLSADYDATITKEKCLENVVKNVQSGS
                     IIVFHDSVKAFPNLEYTLPKALEILNKKGFVFEAIK"
     gene            complement(3581..3877)
                     /locus_tag="PLQ70_04990"
     CDS             complement(3581..3877)
                     /locus_tag="PLQ70_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQE34005.1"
                     /translation="MSKFGELISAQVPVLIDFYTDWNESSVSMHPVIRDVAAALGDKA
                     KVIKIDVDKNQELAEALRIKGLPTLMIYKGGQMIWRQSGELDANTIIGLVQDYL"
     gene            complement(3952..5187)
                     /locus_tag="PLQ70_04995"
     CDS             complement(3952..5187)
                     /locus_tag="PLQ70_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQE34006.1"
                     /translation="MFFRLLILSLVLFIIELYAYQALRTVVKLKPVLISYQIISALLL
                     AYIIYSFTQFDRKIGQTPQTMQTMGLLLMVYLPKIILTLVLFGEDIYRIGFGSINHFA
                     KFNDSVDFLASRRKFVSQIGLGLAAVPFLSLIYGVTKGKYNYKVVKQAIFFPDLPDAF
                     DGFTITQISDVHSGSFDDPDKINYAIDLVNEQNSDMILFTGDIVNTHAKEMHPWIDTF
                     KRIKEHKYGKFSILGNHDYGEYVPWPTEAAKEENFQNIKNLYGQIDFKLMLNEHTFIE
                     KGEDKIALIGVENWGTNFKKVGDINLASANLKKEDFKILMSHDPSHWDCEIQNHPKHF
                     HLTLSGHTHGFQFGIEIPGVIKWSPIQYIYKHWAGLYENMGRYVYVNRGFGFHAYPGR
                     VGIMPEITVIQLKKGKNVA"
     gene            5608..7206
                     /locus_tag="PLQ70_05000"
     CDS             5608..7206
                     /locus_tag="PLQ70_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE34007.1"
                     /translation="MKKLLLFIAIAFVGMESFAQVPTSFLTFRQDFSGNFNNTSSYTT
                     TSDANTATLTTDEFNAASNAGNFDLGQTLEYPFSTNSHLMAGPTQMTISAKVFIDPTW
                     VAGLASNQYVTFLMLGKSYMRLLKSGTGYFLQCGVFNNDPTSGSFGYLSVSGAVIDLS
                     TGWNTITMTYGPEIAPNIGGALRVYVNGAYAGNAMRKAITPDNQPLSYISTSEKLVLG
                     RAVSSNQNFKGKIDRVLIYSKCLTPQEVLGLQNEGQSLTEYTFNNTFNNSKGNEPFFA
                     SNGLTFVNDRNGNPNSALNFTNVNGTQATILNLPYYNYPRTISVWVKPNAIINGAYNY
                     PFFYGTSTNYLLSHYRGGDVTLSDSVNFMSVATTNTAGVWMHYVYVCDTSNIKIYKNG
                     VLLGTMPMTTSTANNNNIFKLGFGTGPGTSLDAALDDLKIYKYALSDTEILNLYNYNS
                     LASQNFQAKISKATIYPNPTSDNFTIEMENEVKSVEIYSLLGQKVLSANTKNTNVSSL
                     PKGVYMVRIEDTNNTTTNQKLIIE"
     gene            7313..8071
                     /locus_tag="PLQ70_05005"
     CDS             7313..8071
                     /locus_tag="PLQ70_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQE34008.1"
                     /translation="MKKIKALIVDDELNARSLLRHFIEDYCPEIELIAEAEDVKSAVK
                     IINKNEIDLVFLDVEMPNENGFSLFDYFNKPTFETIFCTAYSQYAIKAFEVSAVDYIL
                     KPIGIAKLKEAIEKVVEKRNRNAQPNTIAVLKENLAENKIKKIGIHIGDGIVFMDLDD
                     IFYFEADGSYTTIYHKNGKDLAVKKIKHFEDLLSSDNRFFRIHRSYFVNITLIKKYSK
                     KDGLSITYDEKILLPISREKKEEFEEFMQMNNIL"
     gene            8071..9924
                     /locus_tag="PLQ70_05010"
     CDS             8071..9924
                     /locus_tag="PLQ70_05010"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE34009.1"
                     /translation="MKHCSLLLIYLLSFIASAQNNDLIKRINNYPKQDTTRVELLIDY
                     CVDNTFAVSDKMLKFAKEAYTISKKTNYIKGEIRSLNCIGNYYYQQSIFDKAIEFYTK
                     AIRIAEKNKDIENIIIGKSNLASIYIQIKESKKALQLFLESDQLLINTDKEESQNRAA
                     LLTNIGAAYSSLNQHDEALKYHIKVLELCLKKNILFGIALAKSNIGDEYVQLKKYDKA
                     LPFLVSSMEISEKNGFDSFLGQIYSDIGKIYSFKKQYALSTHFLEKGISLCTKINDQS
                     NLLHTNSVLHNVYAQNGNFKKAYLTALNYITVNDSLNNKEKQKIITETNTKYETEKKE
                     TTIKELNQKKKISELESNKKSILINSIVISFLALAIIAYFLFSRFKEKKKNELLQTQL
                     IENQKRLEAEKKATDSELKALKSQMNPHFIFNALNSIQEQFMYGDKLKGNEQLSNFTY
                     LTRQILEVSGKKQITIATEIDILTKYLELEKMRFAKDFEYSITTSETIDEDYMKLPPM
                     LLQPFVENSIKHGLMHKNGLKTIAIDFDLSSSEDYLICTIIDNGIGRQKSAEIKIGNL
                     EKHNSFSTKSIQQRLELLNDTLQLQELIVYSDLLENDSVVGTKVVVYIPLV"
     gene            10156..>10392
                     /locus_tag="PLQ70_05015"
     CDS             10156..>10392
                     /locus_tag="PLQ70_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34010.1"
                     /translation="MSQKRLFLLDAYALIFRGYFAFIKNPRINSKGMDTSAIMGFMNA
                     LMDVIKREKPDHLAVAFDKGGSTYRYEMYQEYKAH"
BASE COUNT         3727 a         1740 c         1629 g         3196 t
ORIGIN      
        1 ggcaacctta tttattgctt tattgttctg gttaggattg cgttgggaac aagatatgga
       61 ttctccaaaa ggaaataaat ggttgttagt catttcgcta gtggttggac ttacatttgg
      121 agttcatttt atggcattat taaccattcc tgctataggt tttctttatt atttcaaaca
      181 ctataaagag gttacagtca aaaattttat tattgccaat atcgttgttg tagctgtttt
      241 attatttatt ttcaagttat tattgccttt aacaatggcc ttttttggta aaactgaggt
      301 ttttatggtc aacgaattag gaatgccatt tgactcagga actattttcg tggttttact
      361 ccttattgcg ttcttttatt ttagtttaaa atacactcga gctaaaggtc taataaaata
      421 caacactcta attttgtgca ttctttttat cctaatcgga ttttcgactt ggatgatgct
      481 gcctattcgt gccaatgcca atacagtaat taacgagaac aaaccatcgg atgcgcgtga
      541 agttttggcc tattataata gagaacaata cggttcaaac cccttgtttt atggcccaca
      601 atatactgaa gcttttgcag gtttagacga aaacaatcct tatttagaca aggctcccaa
      661 ttatgaaaga gattataaaa caggcaaata tgttattgta aataatttca aaaatgccga
      721 gcaaaatagc gatgacaatc aaaaaacctt tttgcctaga ttatggagtg gtgagaatat
      781 tacaagctac attagtttta caaatcctcc tgaatttaaa ctaaatcctg attatgctta
      841 cgaacaggat ttgccaaaat atggcgttga ccccaaccaa ctttcggaag aagatttagc
      901 aaaagcaaca gcacagttaa gaaatgaaat cgaaaaaacg gttgttgaat tcagaaaagc
      961 ttatgctcaa aaacaacttg acaatgacga ctatgtcaaa ttcttaagaa cctacagcga
     1021 ttatttaatt gtagagaaac cctcgacatt tgataatttg agatttatgg ctgaatatca
     1081 atttggctat atgtatttca gatatttgat gtggaatttt gttggacgtc aaaatgacat
     1141 tcaaggaaga tatgattatc aggatggaaa ctggcttagt ggtattactt ttatagacga
     1201 attgcatttg ggcacacaac aaaacttacc gtcggatgta ttgaataaca aaggacgcaa
     1261 tgtttatttc tttttacccc taattttagg tttaatagga ataatgtatc acgctagcag
     1321 agatcgaaaa agcttttatg tactattagt tttattccta tttatgggac ttgctctaaa
     1381 aatttacctc aacgaaagac cttttgaacc tcgcgaaaga gattatgcac tagttggatc
     1441 gttttatgtt tttgccattt ggataggctt tggagtctat tcattatatg aaagtattca
     1501 aaaatattta gctccaaaaa ttgctggacc aattattatt ggcgcaagtt tactagcagt
     1561 tcctgctctt atggcatttc aaaactggga cgaccacgac cgttctggga aatatactgc
     1621 aacagcattg gctaaagcat atctagaatc atgcgatcca aatgctatat tgtttaccat
     1681 tggtgacaac gatacgttcc cactttggta tgctcaagaa attgaacaca tccgaaccga
     1741 tgtcaaaatt gtaaatacaa gtctgtttat gacagactgg tatattgatc aaatgaaaag
     1801 caaaacatac gaatcgaatc cgttgccaat ttcatttaat cacgacgaat atgttggaga
     1861 caaacttgat tatgtagctc atattcaaaa agtagaaagc cgttgggaaa tcaaagattt
     1921 tatcaatttt attaaaaatc caaagtcaac tgttgaaatg caaaatggac aaactattca
     1981 tttctatcca acaaacaaaa tcaggattcc agttgataaa aacaacatta tccagaacaa
     2041 agtggtggat gcgaaattca atgattctat tgttccctac atcgacatcg acatcaaagg
     2101 aaatgcctta tacaaaaacc gattgatgat gcttgacctt gtagctaaca acaattggaa
     2161 aagacctatt tattttagcc ctggcgcatt tgacgacgaa gattatttgt ggatgaaaaa
     2221 ctacctccaa ttagaaggaa tgatttacaa attagtccct ttaaaaacaa aagttgacaa
     2281 agacacaaac cctatggata tgggtggact tgatgctgat aaaatgtatt ctattgtaat
     2341 gaaatgggat tggggcaata gcaatggaga catctatcac gaccctgaaa ctcgaaaaaa
     2401 cagcatttct tatcgttctg ttttatctag actaatggac aaactaattg ccgaaggaaa
     2461 aatggataaa gccaaaaata taatcaactt gtctttaaca aaaatgcctg tcgaaaaatt
     2521 tggttattat tcaatggtag aaccattcgc aaaaggatat taccaaatag gtgaaaaaga
     2581 aaaagcacaa caacttcttg gaaaattgat tggaaaatat aacgaaaact taaattatta
     2641 cggcacttta agctcttctg accaagttga tttagctatt gacatcatta cagacatcga
     2701 acgttacaga agtctattgc acgtaatgaa agaaagtggc gacatccaat tgtacaacaa
     2761 aagccgacca gtattcaaca cctatatcaa aatgtttgaa cgttttggtc gagacagaga
     2821 ataactattt ttaaaaattg aaatcaaaat gtagccttat tgaaaaataa cgctgcattt
     2881 tttttcatct aataaaaact gaataatgaa tttctattgg gtcaaaacaa atcgttttat
     2941 taaaaagcta ttcagcaatt atgtttggga tattcctaat actgaaaata aaatatacct
     3001 cacttttgac gatggaccaa caccagaaat caccgaatgg gttcttgaag aattagaaaa
     3061 acacaatgcc aaagctactt ttttttgcat tgggaataac atcaaaaaac atcctgaaat
     3121 tttcgaaaag ttaatctcca agggacattc tattgggaat cacactttca atcatttgaa
     3181 tggttggaaa acttcgactg acgattattt agaaaattct nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn tctcaggcaa aaaaacttaa acaattaggc taccaaataa
     3361 ttatgtggga tgttttaagt gccgattatg atgcaacaat taccaaagaa aaatgtttgg
     3421 aaaatgtagt taaaaatgtt caatcaggaa gtataatcgt atttcacgac agtgtaaaag
     3481 catttccaaa tttggaatat actttgccaa aagctctgga aattttgaat aaaaagggat
     3541 tcgtttttga ggcaattaaa taaattgttc ttgctttcaa ttaaagataa tcttgaacca
     3601 agccaataat agtattagca tctaattctc ctgattgtct ccaaatcatc tggcctcctt
     3661 tgtaaatcat caacgtagga agtcccttga ttcgcaaggc ttcagcaagc tcttgatttt
     3721 tatccacatc aattttaata acttttgcct tgtctccaag agctgcggca acatctcgaa
     3781 ttactggatg catcgaaact gatgattcat tccaatctgt gtaaaaatca attaacacag
     3841 ggacttgcgc acttataagt tctccaaatt ttgacataaa accaacgaat ttaattttct
     3901 aaatctattg aaaagagata aatataatac aaatgtagca ttttaaacga attatgctac
     3961 attcttacct ttttttagtt gaattactgt aatttcaggc ataatcccta ctctaccagg
     4021 ataggcgtga aatccgaaac ctctgtttac ataaacatat cttcccatat tttcatacaa
     4081 accagcccaa tgtttgtaaa tgtattgaat aggactccac ttaattacac ctggaatttc
     4141 aataccaaac tgaaatccgt gcgtatgacc tgaaagcgtt aaatgaaaat gcttgggatg
     4201 attttgtatt tcacaatccc aatgacttgg atcgtgactc atcagaattt taaaatcttc
     4261 ttttttcaaa ttggcagaag ccaaattaat atctcctact ttcttgaaat tagttcccca
     4321 attttcgaca ccaatcaaag caattttgtc ctctcctttt tctataaaag tatgctcatt
     4381 caacattaat ttgaaatcaa tttgaccgta taaattttta atgttttgaa aattttcttc
     4441 tttcgcggct tctgtaggcc aaggaacata ttcgccataa tcatggtttc ctaaaattga
     4501 aaatttaccg tatttatgtt ctttaattct tttgaaagtg tcaatccaag ggtgcatttc
     4561 tttagcatga gtgttgacta tatcacccgt aaacaaaatc atatccgaat tttgctcatt
     4621 gaccaagtca atagcataat tgattttatc tggatcatca aaacttccgc tgtgaacatc
     4681 ggatatttgt gttatggtaa aaccatcaaa agcatcgggc aaatcaggga aaaatatggc
     4741 ttgtttaacc actttataat tatactttcc ttttgtaaca ccatatatta aggacaaaaa
     4801 aggaacagca gcgagtccta aaccaatttg actaacaaac tttcgtcttg atgcaaggaa
     4861 gtcaacgctg tcgttgaatt ttgcaaaatg gtttatagac ccaaaaccaa ttcgataaat
     4921 atcttctcca aataatacta aagtcaaaat aattttagga agataaacca tcagtagcaa
     4981 acccatagtt tgcatggttt gcggtgtttg tccaatcttg cggtcaaatt gtgtaaatga
     5041 ataaataata taggctagaa gaagcgcact tattatttgg taggaaatta gaactggttt
     5101 taattttaca acagttctta atgcctgata agcatagagt tctataataa aaagcaccaa
     5161 cgatagtatt aacaagcgaa aaaacattat gatttatttt ttgtgcaaat taacgaagaa
     5221 tgaatacacg aaataacttc attataaaaa ctttaactac aaggaggatt gcaacaaaaa
     5281 attaccattc gtgaaagaaa agaagctttt catgaaaatc caatttttat tcaattaata
     5341 tccgtgactt ttgactgaaa ttgaacaaga aaaaaataaa atgaacaaga aaaaaaactt
     5401 tatccaaatt atcttaaaac gattatttct aatcataacg agatgaaaaa attgggtttt
     5461 aggcaatgac tgttattttt caatcgagta ggttttttta gaatcaaaac catctgttcg
     5521 tgaaaaatat ttggagtttc gtggataaaa attccctcgc aaaactgttt tattgacttt
     5581 tgaatgaaat ttttaaaact acttattatg aaaaaattat tactttttat tgcaatcgct
     5641 tttgtaggaa tggaaagctt tgcacaagta ccaaccagtt tcttaacttt tagacaagat
     5701 ttctcgggca actttaacaa tacttcaagt tacacaacaa catcggatgc aaacacggca
     5761 acactaacca cagatgaatt taatgctgct tctaatgccg gaaattttga tttaggacaa
     5821 acccttgaat atcctttttc gactaattct cacttaatgg ctggcccaac acaaatgaca
     5881 atttcagcaa aagtatttat agacccaact tgggttgcag gattagccag taatcaatat
     5941 gtcacttttt tgatgttagg aaaatcgtat atgcgccttt tgaaatcggg taccggatat
     6001 tttttacaat gtggcgtttt caacaatgac cctacctctg gatcgtttgg ttatctaagt
     6061 gtatcgggcg ctgttataga cctatctact ggctggaaca ctattacaat gacttatggt
     6121 ccagaaattg ctccaaacat aggtggtgcc ttgcgtgtat atgtaaatgg agcatacgca
     6181 ggaaacgcta tgagaaaagc aattacacct gacaaccaac ctttaagcta catcagtact
     6241 tctgaaaaat tagttttagg aagagccgtt tcttcaaatc aaaattttaa aggaaaaatt
     6301 gacagggtgc taatttacag caaatgcttg acaccacaag aggttttagg cttgcaaaat
     6361 gaaggacaaa gccttacgga atatacattc aacaatacat tcaataatag caaagggaat
     6421 gaaccttttt ttgcatcaaa tgggcttact tttgtgaatg acagaaatgg aaacccaaat
     6481 agtgctttaa attttaccaa tgttaacgga acacaagcta ctattctaaa tttaccttac
     6541 tataattatc ctagaacaat ttcggtttgg gtaaaaccaa atgcaatcat aaatggtgct
     6601 tacaattatc cattttttta cggaacaagt acaaattatc ttttatcaca ttaccgagga
     6661 ggcgatgtta ctctttctga ttcagtaaat tttatgtcgg ttgcaacaac caatacagct
     6721 ggcgtttgga tgcattatgt atatgtttgt gatacatcaa atataaagat ctacaaaaat
     6781 ggcgttttat taggcacaat gccaatgact acatcaactg ccaacaataa taatatattt
     6841 aaattgggtt ttggcacagg cccaggaact agtttagacg ctgctttaga cgatttaaaa
     6901 atctataaat acgcattaag tgataccgaa attttaaact tatacaatta caattcattg
     6961 gcaagccaaa acttccaagc caaaatatca aaagcaacca tctacccaaa tccaacttca
     7021 gataatttca ctatcgaaat ggaaaatgaa gtaaaatcgg ttgaaattta ttccttactt
     7081 ggacaaaaag tgctttctgc aaatactaaa aacacaaacg tttctagttt accaaaagga
     7141 gtttatatgg ttcgaataga agacacaaat aacacaacta ctaaccaaaa attaattatt
     7201 gaataaactc aaatagtcat ttttttttga aaatccgttc cgaagattaa tttctgctgg
     7261 agcggttttt cgattaaaaa aaatccttat tattgttgaa tcaattattt gtatgaaaaa
     7321 aataaaagcc ctaattgttg acgatgaact gaatgcaaga tccttattgc gccattttat
     7381 cgaagactat tgtccagaaa tcgaactcat tgccgaggca gaagatgtaa aatcggctgt
     7441 aaaaatcatc aacaaaaacg aaattgattt agtttttttg gatgtagaaa tgcccaacga
     7501 aaatggcttt tcattattcg attatttcaa caaacccaca ttcgaaacca ttttttgcac
     7561 cgcttattcg caatatgcta tcaaagcctt cgaagtttcg gcggtagatt atatcttaaa
     7621 acccattggc attgccaaac tcaaagaagc gattgaaaaa gtggttgaaa aacgaaatcg
     7681 gaatgcacaa cccaatacta ttgctgtttt aaaagagaat ttagccgaaa acaaaatcaa
     7741 aaaaattggc attcacattg gcgatggaat tgtttttatg gacttggacg acatctttta
     7801 ttttgaagcc gacggttctt acaccaccat ttaccacaaa aacggaaaag atttggcggt
     7861 taaaaaaatc aaacattttg aagatttact ttcttcggat aatcgctttt ttaggattca
     7921 tcgctcctat tttgtaaaca ttactttgat taaaaaatac agcaaaaaag acggtttgtc
     7981 aatcacttat gatgagaaaa ttttattgcc tatttccaga gaaaagaaag aagagtttga
     8041 agaatttatg caaatgaata atattttgta atgaagcact gttccctttt attgatatat
     8101 cttttatcat ttattgcatc tgcacaaaat aatgatttaa taaaaagaat aaacaattac
     8161 ccaaaacaag acacaacaag agtagaatta cttattgatt attgtgtaga taatactttt
     8221 gcagtttcgg ataaaatgct aaaatttgcc aaagaagcct atacaatttc caaaaagact
     8281 aactatataa aaggagaaat acgctcacta aactgtattg gaaattatta ttatcagcaa
     8341 agtatattcg acaaagcaat cgaattttat actaaagcaa tacgaattgc cgaaaaaaat
     8401 aaggatattg aaaacattat tattggcaaa agcaatttgg ctagcattta tattcaaatc
     8461 aaagaatcta aaaaagcgtt acaactattt cttgaaagcg accaattatt gattaataca
     8521 gacaaagaag aatctcaaaa tagagccgca ttattaacca atattggcgc agcctattct
     8581 tctttgaatc aacacgacga agctttaaaa taccatataa aagttttaga actttgccta
     8641 aaaaaaaata tcctttttgg aattgcttta gcaaaaagca atattggcga tgaatatgtt
     8701 caactaaaaa aatatgataa agctcttcct tttttagttt cgtctatgga aatttcagaa
     8761 aaaaatgggt ttgatagttt tctgggtcaa atttacagtg atattggaaa aatatattcg
     8821 ttcaaaaaac aatacgcact ttcaactcat tttttagaaa aaggaatttc actttgtact
     8881 aaaataaatg accagagtaa tcttttgcac accaacagcg ttttgcacaa tgtttatgca
     8941 caaaatggta atttcaagaa agcttacttg acggcattaa attacataac cgtaaatgat
     9001 agtttgaaca acaaggaaaa acaaaaaatt attactgaaa caaataccaa atacgaaacc
     9061 gaaaaaaaag aaaccacaat caaagagttg aaccaaaaaa agaaaatctc tgaactagaa
     9121 tccaacaaaa aaagcatcct gatcaatagt attgtaattt cgtttttggc attggcgatt
     9181 attgcttatt tcttgttttc gagatttaaa gaaaaaaaga aaaatgaact tttgcaaact
     9241 caacttattg aaaatcaaaa acgattggaa gccgaaaaga aagccaccga tagcgaactc
     9301 aaagccttga aaagccaaat gaatccgcat tttatcttca atgcgctcaa cagcattcag
     9361 gagcaattta tgtatggcga caaactcaaa ggcaacgaac agttgagtaa tttcacctat
     9421 ttaacccgac aaattctaga agtttcaggc aaaaaacaaa ttaccattgc taccgaaatt
     9481 gacattttga ccaaatattt ggaactcgaa aaaatgcgtt ttgccaaaga ctttgaatac
     9541 tcgataacca catctgaaac tattgatgaa gattatatga aattgcctcc aatgttgctg
     9601 caaccctttg tagaaaacag catcaaacac ggactgatgc acaaaaatgg cttgaaaaca
     9661 attgcaattg atttcgattt gagttcaagc gaagactatt taatttgcac catcatcgac
     9721 aacggaattg gtcgccaaaa atcggcagaa attaaaatag gaaatctcga aaaacacaac
     9781 tccttttcta caaaatcaat acaacaacga ttggaattat tgaatgatac tttgcaacta
     9841 caagaactta ttgtctattc agacctccta gaaaacgata gtgtagtggg tacaaaagtg
     9901 gttgtgtata ttcctttggt atagctttac aaacaaaata aattatacac attcaacccg
     9961 attattacca atattacgaa gactaatagt attattttgt aattcaaatt tttaataatt
    10021 actgacatac aacttgattt ttcagtaaaa taaagattct ggaatcaaca aataatcgaa
    10081 aaatttatga aaggtagctt ttagttcacg aaatgaggat ttgtttgtta tttttacacc
    10141 tcaaatttta tttcaatgtc acaaaaacgc cttttcctgc tcgatgctta cgctttaatc
    10201 tttcgcggtt attttgcttt tataaaaaac ccaagaatca attccaaagg aatggatact
    10261 tcggccatta tgggttttat gaatgccttg atggacgtta tcaaacgcga aaaacccgat
    10321 catttggccg tagcttttga caaaggcgga agcacctatc gctacgaaat gtaccaagaa
    10381 tacaaagcgc at
//
LOCUS       DAOSNN010000067        10359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6448_length_10359_cov_5.537267, whole genome shotgun sequence.
ACCESSION   DAOSNN010000067 DAOSNN010000000
VERSION     DAOSNN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10359
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6448_length_10359_cov_5.537267"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PLQ70_05020"
     CDS             complement(<1..1131)
                     /locus_tag="PLQ70_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein RemB"
                     /protein_id="HQE34011.1"
                     /translation="MKKGFLTLLSTLCLFVTFAQEVNDKKQTSFSAEQFPVFPKCENL
                     QSKELEKCFYNQVQDFVFENFVVPENLVQNNFKGNVKVLFEVDKNGSFNVIYVNAVDD
                     ALIQETKRVFAKFQKIKPSTYNGNPTFAQYNITIAIPLKSAEQMTAETVVVAETAKAI
                     PKQLTELDSIVYKKFNNPQFNSHLNVPFSHSYYAHFDGAMNQVGANNHTASKPYSYVE
                     VAKYYNIAEENEKIKKNKTGWWGRKLWNENLVEIQGEDYWLLLNPIFDLQVGSASGNK
                     HVNTFTNTRGINLSGGLGSQLNFTTTIYESQGRFADYYNRYAESIRPAGGNPAIIPGI
                     GIAKDFKTDAYDIPSADANLTFAPSKFIDLQLGYGRNFIGDGY"
     gene            complement(1147..1890)
                     /gene="deoC"
                     /locus_tag="PLQ70_05025"
     CDS             complement(1147..1890)
                     /gene="deoC"
                     /locus_tag="PLQ70_05025"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQE34012.1"
                     /translation="MNIKQYLDSTYLKTPSQAGLNEAENTGIVKEFIQEAIDENFKLI
                     MIRPNMVALANQMISQANSAVQIGTVISFPEGTAGIEAKLAEATQAIHDGADDLDFVC
                     NYEAFKAGNVDLVKEEILKGTQLGLAHNKVVKWIIEVAALTDKQIMQLSALIKNIVIT
                     NFKEENYASVFVKSSTGFYKTENNLPNGATVPAIIIMLENASPLPVKAAGGVRTYEEA
                     VEMIRLGVKRIGTSGAKAIANGQKASGEY"
     gene            complement(1902..2288)
                     /locus_tag="PLQ70_05030"
     CDS             complement(1902..2288)
                     /locus_tag="PLQ70_05030"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQE34013.1"
                     /translation="MRRTIFLYSAIFWTGIILFLSLENANEIKQIEIPNFDKLIHAVF
                     HFVFTTLWFLYLKKKLVTITNFRLLSFALMGSFFFGIAIELMQQYFTTTRSADVLDVL
                     ANLFGASLAVISIILLNNYNRFIDKI"
     gene            complement(2281..2661)
                     /gene="gcvH"
                     /locus_tag="PLQ70_05035"
     CDS             complement(2281..2661)
                     /gene="gcvH"
                     /locus_tag="PLQ70_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQE34014.1"
                     /translation="MNVPANLKYTKDHEWVSIEGDVATVGITHFAQKELGDIVYVEVE
                     TLDQTLDKDEVFGTVEAVKTVSDLFLPLSGEIFAFNDDLESNPESVNSDPYGAGWMIK
                     VKISNPAEIDSLLSSESYKELIGA"
     gene            complement(2740..9888)
                     /gene="sprA"
                     /locus_tag="PLQ70_05040"
                     /pseudo
     CDS             complement(2740..9888)
                     /gene="sprA"
                     /locus_tag="PLQ70_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137496.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
     gene            complement(9925..>10359)
                     /locus_tag="PLQ70_05045"
     CDS             complement(9925..>10359)
                     /locus_tag="PLQ70_05045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HQE34015.1"
                     /translation="LQIKEDSHTLFGFVEKSEREIFKLLISVSGIGASIARTMLSSLD
                     PKQITNAIASGDVVTIQSIKGIGSKTAQRVILDLKEKVLKLYDLDEVSISQNNTNRDE
                     ALSALEVLGFVRKASEKIVEKIIKDAPESSVEYIIKQALKNL"
BASE COUNT         3175 a         1898 c         1661 g         3625 t
ORIGIN      
        1 atacccatcg ccaataaaat ttcgaccata acccaattgc aaatcaataa attttgaggg
       61 tgcaaaagtc aaattggcat cagccgaagg aatatcataa gcatcagttt taaaatcttt
      121 tgcaattcca attccgggaa taatggctgg atttccaccg gccggtctaa tagattcggc
      181 atatcgattg taataatcgg caaaacgacc ctgactttca taaatggtgg tggtaaaatt
      241 aagctgcgaa cccaatccac cgctcaaatt aattccgcga gtgtttgtaa atgtattaac
      301 atgcttgttt ccagaagccg aacccacttg taaatcgaaa attggattta agagcaacca
      361 ataatcttcg ccttgaattt cgaccaagtt ttcgttccac aattttcgtc cccaccagcc
      421 tgttttattt tttttaatct tttcattctc ttccgcaata ttgtaatatt tagccacttc
      481 tacataagaa taaggtttag aagcagtatg attattggct ccaacctgat tcattgctcc
      541 atcaaaatga gcataataac tgtgtgaaaa agggacattc aaatgactat tgaactgcgg
      601 attgttgaat tttttataca caatactatc caattcggta agttgtttgg gaattgcttt
      661 tgccgtttca gcaacaacaa ccgtttcggc tgtcatttgt tcagcgcttt ttaagggaat
      721 tgcaatggta atattatatt gtgcaaaagt tggatttccg ttataagttg agggtttaat
      781 tttttgaaat ttagcaaaaa ccctttttgt ttcttggatt aatgcatcat caacagcgtt
      841 tacataaatc acattgaatg agccattttt atccacttca aacaggactt ttacatttcc
      901 tttaaaatta ttttgaacta aattttcagg aactacaaaa ttttcaaaaa caaaatcttg
      961 aacttggttg tagaaacact tttctaattc tttggattgt aaattttcac attttggaaa
     1021 aacaggaaat tgctcggcag aaaaactggt ttgtttttta tcatttacct cttgagcaaa
     1081 agtaacaaaa aggcaaagag tagaaagcaa tgtcaaaaat ccttttttca taatgtgtct
     1141 atagttttag tattcgccag aagctttttg tccattagca atcgctttgg ctccagaagt
     1201 acctattcgt tttacgccca atcgaatcat ttcgactgcc tcctcataag ttctcactcc
     1261 gccggctgct tttacaggaa gtggagaggc attttcgagc attataatta tggctggaac
     1321 cgttgctccg tttggcaaat tattttcagt tttataaaaa ccagtggatg attttacaaa
     1381 aaccgaagca taattttctt ctttaaaatt agtaataaca atgtttttaa tcaatgccga
     1441 aagttgcata atttgtttat ctgtaagtgc tgccacttcg ataatccatt ttacaacttt
     1501 gttatgagcc aaacctaatt gagttccttt taggatttcc tcttttacca aatctacatt
     1561 tcctgctttg aaagcctcgt aattacaaac aaaatcaagg tcgtcagctc catcatgaat
     1621 ggcttgagtc gcttcggcaa gtttggcttc aattcctgct gttccttctg gaaaactgat
     1681 tacagttcca atttgcactg ccgaatttgc ttgagatatc atttggtttg ccaatgcgac
     1741 catgttgggt cgaatcataa tgagtttgaa attctcatcg atagcttctt gaataaactc
     1801 tttgactata ccagtatttt cggcttcatt aagtcccgct tgagaaggcg tttttaaata
     1861 agtagaatct aaatattgct tgatattcat tagtgtaaaa attaaatctt atctataaat
     1921 ctgttgtaat tattcaacaa aataattgaa attacggcca gagatgcacc aaatagattt
     1981 gccaaaacat ccaaaacatc ggcgcttctt gttgttgtaa aatactgttg catcaattca
     2041 attgcaatac caaaaaagaa ggaacccatt aatgcaaaac tcaaaagacg aaagttagta
     2101 atagtaacta atttcttttt taaatacaaa aaccaaagtg ttgtaaagac aaaatgaaaa
     2161 accgcgtgaa ttagtttatc aaagtttggt atttcgattt gtttgatttc gttggcattt
     2221 tctaaactca aaaacaaaat gattccggtc cagaatatgg ctgagtataa aaaaatagtt
     2281 ctacgcacca attaattctt tgtatgattc actagaaaga agagaatcaa tttcggctgg
     2341 attagaaatt ttcactttaa tcatccatcc cgctccataa ggatcagagt ttacactttc
     2401 aggattgctt tcaagatcat cattaaatgc aaagatttcg cctgataatg gaagaaacaa
     2461 atctgaaact gtttttacag cttcaactgt tccaaaaact tcgtctttat ctaaagtttg
     2521 gtctaaagtc tccacttcaa cataaacgat atctcccaat tctttttgtg caaaatgagt
     2581 aattcctact gttgcaacat caccttcaat gctaacccat tcgtggtctt ttgtatattt
     2641 taaatttgct ggtacgttca ttttaatatg atttaagttt ttttttaatg gcacaaatgt
     2701 aacaatcttc tgttcaaaaa tagttccgtt ttcaaaaaat taattcccaa aattatatcg
     2761 aagcgtaaaa ccagaacgga tgttggttaa tggaaaagaa gttgatatta ctgcttgcga
     2821 aaaggcatgg tcataataga aaatagcggt tagattttta ctaaaagaat aatcagcggt
     2881 tagtttgagc gaccaaatat tttgtcctcc agccaattga ttgttgtcat aatctaaata
     2941 tctcacgata gttttgttgt ttcgatacga caaatcggct tttattataa tatcactttt
     3001 tataattcct gtaggattat cggcaagttg agaagaaaaa accacgtctt ttattctata
     3061 tcccattcca actatatatt ctatgccttt tacttcggtt aataaattgt tgtcaaaact
     3121 catcgataat gctctgtctt ttttgatttc ggtaagcaat tttaaggagt ttttcaattc
     3181 gaaatccatt cgaagcaacg gattgaactg ctcgactaaa ttgacgttag acataatggt
     3241 tttgttaaag aaattgccac ttgcatcgac tccacttggg tttttatcaa attcaaaatt
     3301 ggatctaaaa gcattaattg tataagaagc tctatagttt tgttgcaatg agaatcgttt
     3361 gaaattcttt ttgaagaatt cataacgcat caatccatta tatttgatag accaattggg
     3421 taccggaaaa cttttgaaaa ttcctgtaga aacacttgag gcactttgcc cagaataggc
     3481 tgctaaaaag gctggtaata aaacggcctg attgtttttg ccatatcctt ttggaaaacc
     3541 atctgcgtct ttaatagttg gatcatataa aggatcacgt ttttggtgct caattgccaa
     3601 tcgattagca acctcaagcc tattacttct aaaatcatca aaagctgctg atgaaacttc
     3661 atcgcttgct gagaatgaag tttttatcaa aaccgtagaa atagaaaaca ttccatagct
     3721 ataaggagat cttggattgt aaattccgtt ggtaacgtca tattgttcag aaaaattctc
     3781 agaataagat ctgtctaacg acaaatctat ttttaaatcg ggcaacaaat ctatgtttac
     3841 cgttgattta aatacttcat tgcttacctg agtataattt tgattaaagt cggaataagt
     3901 cgtaagccaa ccgttttttg cggcttcata tcttacatca tcttggcttc caaaaataaa
     3961 tccaagtgtt ggttttgaag aaccaaagaa tccaattcct ggcgtatatc ctggcaaaac
     4021 ggtaccacta tttttagtat aattggcctg aatatttttc acacttgtta aaatgcctat
     4081 caaaccatta tagaaattgt tttgttttgg aactaacgca ttcgtattgg ctactttttc
     4141 acctggttta ggagcaactg ctttcgggtt tggttttaat tgtgtttttt gagttaatcc
     4201 tatgtactta taaaatgtat ccattgaaag cgtggcattc aaattatgag acattgcatt
     4261 ttgaattgtg tttcctaaat tataagtagt tccatctgct gcggtatatt cagacaatgc
     4321 cactgaagca cgttgccaat tatagtcgcc tgtgtaagaa taattagctt ttatgaaact
     4381 taacaaaggt atcttgttaa ttggaatttc ataattcatc accaattgtt gcatgtgttg
     4441 attagcattt ccaatatcta aataactatc ccaaatattc aaatcatcgt tgggcacatt
     4501 atcatcattc atgtagtttt ttacaatatt ggttgaagat gcagtgtagg taaattttaa
     4561 atttttagtc aaattataat taaacccata attataatta aatgcataat tccttctgta
     4621 cattggatcg ataccaattc caacaacatc tacttatcta aattgttgac gattgtattg
     4681 tctattaata ttagtattga aattaatatt ggttggcaaa taattaaaat taaagtcact
     4741 taatgcttta taatagctgc tttttttagt gaattttgat tttttgaaag gttcaattgg
     4801 tttcggctgg aaactaaaag catagtcaac agtagtattc gattcttgat ttgtatattg
     4861 ctcaatttca taatcgtgat gctctacttc gttatatgac tgcgaaaatg taaagttttc
     4921 agcatcatat actctttgtt tttgttcagg acctctttct tttcttacac caataaagtt
     4981 gatgcttttt cttttggtat aatcaactgc tctgtttttg atattgtctt tttcagcttc
     5041 atcagtagta ttgtctaata actgtttcag cttaatatcg gcattaaacg gatcatattc
     5101 tggagtgatt gtttcttctc caatggcgta attaaaaggt aaagtgattc tccattttgg
     5161 tggcaataat ttacccatac taaaattagt tacaatatta tactgctgag tatcctcacg
     5221 atttctttcg tttggtcctt gttcaatacc tccaaaacca atggtgcttt ttctacctgt
     5281 ggccgaaacg gttgcgaaat cggcaaaatt ggtatccaca tttaatacag cagccatacc
     5341 tcgactattg tccatatcgg caacgcgaag ttcgtcaaac caaacctccc ctttaatatc
     5401 ttggtggagg tcattatttt ttacacccaa catcaaattt cgaactaatc cgaaatttgg
     5461 atttccttta attcctatcc ttaatttatt agcttttcct gccaatgaag gatctaaatc
     5521 ttcttcattt tgataatata ttccatcagc tggcagtgta cttggatcaa tactcattgc
     5581 ttgaattttc aatttggtca ataatgccaa tgaaaggtca atttcatttt cggcaggcca
     5641 aacgacttcg gcactaagcg cgccacagtt tgaacctaca gaacccgaag gcaatgttac
     5701 ttttaaaggg atttcaattt gatagaaatt ctgagtatag tcgtttccaa agcggataaa
     5761 acctaccatt tgatcatcac gaagctgaat ttcgcttggc aacgattcgg catgcaaaaa
     5821 cattttcagt tttttgaact gacgcatatc gacactgaca tttttaaaca cggctcttga
     5881 atcttctggc tctaatccat cgcccgaaac tcttaatgat aatgattgct cattttgatt
     5941 gataaccgta ttaccactat acaattgctc ttgttgcaca cccggaggta tgatataatt
     6001 tattggacat ctatcattat tttcctgaag atttactgcc aaaacatcca aatttgtttt
     6061 gtcatcggcc gggttgggat catttgaatc caaggtgttt gtatatcttc tccaatctcc
     6121 tcttactaaa tctaaagctc caaaacgaac ggtaacatca ctgctaaaac cggtcataaa
     6181 cattctcatg aatcgaatcg atctaaaatc agaaatattt ccaatagtat tttcgggttg
     6241 cgaaactggg attttgaatt gtaaccatct tgcatctgtc acttgcccat tgtctaaggt
     6301 aacctgagta tttctaatat cagtaatata attttgacct acactcatat tgggtttcac
     6361 atctacgcta tattcataat aggcattgat ggtgttcatc gtattatctc tattgatatc
     6421 ttcaacatct gggtcagaag ttgctgctct gttggcatca ttaatatcaa ccgcagagtt
     6481 tccttccact ccattataat ctctgtatct atctacaact cctccacttg cattcaaata
     6541 atatttgtaa ttatctgcag ctggatcggg atcagaggca taatttttat aaatatctcc
     6601 ttctttagca ttttctaaac cgtctaaacc taaatcttga ctggttctat ttgcagcatt
     6661 ggcatcgaat gcataaatca aggattgcga agctggaaca tctccccaaa gtggctgagg
     6721 attaacttta acttgatctg gccccaatcc attttcgtat tgttttcttc cgtcttttaa
     6781 aacgtcttcg gaaatttcac ctaaattaaa ataaattttt ccagtattgg tctttggaat
     6841 ttcacctgca ccaacataag ggtctaaaac ccaaaactga atgtattcaa catttccttg
     6901 ttcgaaattg gtagaattta gcgaacgcat aatccctcca aaattatctt tggcattggc
     6961 atcaacattc aaagcgttat tataaggacc tctttcggca ggataatagc tcaaatctaa
     7021 agtgtttact acttgtgatt gtccttgtgc aatatcagtt agtggatata attcttcgct
     7081 aaaaattctc cttgtagtat taagcgacaa atcgtcgttt gaaattcctg atggttttgt
     7141 tgtataaaaa acggggtcaa tggtgtacca agacattttg gctcttttga aaccataact
     7201 caaatcgttg gcacttgcat taaaattata caaactttcg gtatcattag ttggagttga
     7261 agataatttc caagcgtgag gagagcgcat atcgatagta gattgcgacc cttcaaaatc
     7321 atctacatat atggttgatt ctccctgaaa actattccct ttaggatctt gaggttctaa
     7381 aaatgcgatt tcacctctta tagaaagatt cgaaggaaca tcagtatcca tattaggtaa
     7441 tttgtttacc aatcgagtaa aaaatggaat ttcggaagaa taatttccat tgaaaccata
     7501 catattattg ttaaccgttt cttgtccaaa gctagatttt tgagtaaaag gtctttcgtt
     7561 caatttcaag aaagtggctc caaggagaaa cttgtctgaa atttgatgct ctacataaac
     7621 cccaaaaaag cgtttggttt gttgtccaaa aattgaattg ttctctaatg aaacgctaat
     7681 tggcgtgttc gatgcttgaa gagatggatc tagaatttga acccttccta gttgataatt
     7741 gacactataa tcaatccctt ctactaaaac tcttccgcca gctgttacaa ctaccgaacc
     7801 tcttggcacg ttgaaagccc caattggaat cccatcgcct cctgtagatt tgtatttacc
     7861 tcttaacaag aatttgtttt tgtcgctgtc ttgcaaagct cccgattgtg tattagaata
     7921 catatttcgg aacacatatt tcttttggtt ggcattataa gtatttacat ccttataatt
     7981 ttctcctgtt cctgtagata atttagagaa caataattcc ccgaaaggct ctttggtagt
     8041 aaatataatt cgaccatttt gagtgtcaat ggttaaacct tgcataaaat cgaagaaacc
     8101 atcaccaccc acttgagggt cattattgaa atttaattta tctaaattaa atactttcaa
     8161 caatggtgtt tcggctactt catttcctac ggatggattg ggcgggaaag tacttcctga
     8221 aactggtgta atataattta ttggagaagg atcagtataa aggatattga atctgaaatt
     8281 ttcttgtttc aattgatagg ctcccggaat ttgataaatg tttttcatca tcaaattcca
     8341 aactggattt ttaacattgg tcaaattact tttcagcatt ttcaacacca aactttgcga
     8401 aataatggct tgatcagatg gagttgttcc ggttacaatc gttgcatcga ccccatcgtt
     8461 accaaattca cctacttgat agacttgatc accaatggtg tattgatatg caacggcaag
     8521 aacttcatca ttagcaagac gttgttggag cgaaatatat cccaattgag gattgaaagt
     8581 atattcgttt gcatttaatt ttctagcatt ttctaatttc gaataatcgg caccctcatt
     8641 aacattagca ttaaatccag aacttgaagt agcaatttca cgaatattat tgttcaacaa
     8701 acctgttcca gctgcaattt gtgctggatc ataatcatta tttgtattgt cggcaggaga
     8761 atcagccgga ttattaaaca tccctgttga cgggcttaaa actactactt cgctatcttg
     8821 caaacccgtt aattgggctt cacctaaatc ctgaatagca ataatatttc taagattgtt
     8881 attggtagtg gttactcggt tttgcttatt agtaacccaa acctcaattc tagaaatttg
     8941 aactctacta tcaataaatg gatagtttct taaggccgca tcgtatttat ttctgaaata
     9001 ttgcgaaaga aaaaagtgtc gatcattatc ataatctagt gcaaacatat caaaatcttg
     9061 aaccgttcca ccaccttcgg caacaacagt tttggtctgt gatttttgtt cagagaaaac
     9121 acctgtaata ctggttttgc caaactgtaa tttggttttc accccaaaaa ggctttgagc
     9181 tccttttatc aaagagctat tcaaaggcat acttacattt cctacttcaa ttttttgaac
     9241 aatatcgtct tcggtaggcg tgtattcgag cttgattaaa ttttgaaatg caaaagtaga
     9301 ttgggtgtca taattgacat ttacattgag tcgggttcct actttcccca tcaaactcat
     9361 acttattctt tgattaaaat caaaagaaag tgttgatcta tttctaggag aaaaggcagg
     9421 attgtcttgt tttgtataac gcataccaaa atccatttca atagatcctg ttggttttat
     9481 atcaatagta ttacttccaa aaatagtttc aaaaagtcct gatttaatat agtaacgggg
     9541 taataaatcc tttttggcaa gatcattgtc tccatttttt ccatcaatag cgtctaattt
     9601 ttctttaaaa tagctgcgca tcgattcctt tcttactcta tcttcaaatt cttttggaga
     9661 caaaattgca ggataacttg ttggaaaatc gccaatgttt tttgacaaaa tatacattcc
     9721 cgtaacaggg tcataggaat acgcttttac aatacttggc gggtctttaa tttcgatttt
     9781 tcctgtggaa tacccagatt taacagtatc ctgagttgta ggatttactt gagcctgcga
     9841 tacaaatcca caaaacatta gtattaaaaa cgcacaaatt ttatacacgc ttgggttctt
     9901 tttaaaattg ggtaacttca agttttataa gttttttaaa gcttgcttga taatatattc
     9961 tactgaactc tctggagcat ctttaatgat tttttctact attttttcag aagcttttcg
    10021 aacaaaacct aaaacctcca aagcagataa cgcttcatct ctatttgtat tgttttgcga
    10081 gatcgaaact tcatctaaat cataaagttt tagcactttt tctttcaaat cgagtattac
    10141 tctttgagcc gttttactgc caattccttt tatagattgt atagtgacca catcacctga
    10201 tgcaattgca tttgtaattt gtttgggatc taatgaagac aacatggttc tggctatact
    10261 tgctccaatt ccagaaaccg atatcaataa tttaaatatt tctctttctg atttttctac
    10321 aaatccaaat aaagtatggg agtcttcttt tatttgaag
//
LOCUS       DAOSNN010000068        10286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6503_length_10286_cov_6.223732, whole genome shotgun sequence.
ACCESSION   DAOSNN010000068 DAOSNN010000000
VERSION     DAOSNN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6503_length_10286_cov_6.223732"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..295
                     /locus_tag="PLQ70_05050"
     CDS             65..295
                     /locus_tag="PLQ70_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34016.1"
                     /translation="MELPKFLLGDNTDFPDDIFIIHLDYPRFIINLKDDEVEFMEEAE
                     DLDEAELNAEMEGLIEQANAFYDREMERYEKE"
     gene            complement(358..1452)
                     /gene="meaB"
                     /locus_tag="PLQ70_05055"
     CDS             complement(358..1452)
                     /gene="meaB"
                     /locus_tag="PLQ70_05055"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQE34017.1"
                     /translation="MDNKNQHPSSLHEKDGISPPEIISSKAVEQIQLLRKKQRTTQEL
                     INGVLAGNISDLSRAVTLIESTNANHLAKANEVLNACLPHANKSVRIGITGVPGVGKS
                     TFIEAFGKHLTGLGKKVAVLAVDPSSTISHGSILGDKTRMEELIKDPNAYIRPSSSGE
                     TLGGVARKTRETIMLCEAAGFDTILIETVGVGQSETAVHSMVDFFLLLKISGAGDELQ
                     GIKRGIMEMVDAIVINKCDGDNIKKSKLAKTEFERALHLFPAKKSGWIPTTSTCSSIT
                     REGIPEIWQTIEQFIEITKGNTYFFEKRAAQNQYWLLETINEQLKTNFYNNPEIQKVL
                     ESTKKAVQNDEISPFAAAQFVLENYFKNLK"
     gene            1679..2590
                     /locus_tag="PLQ70_05060"
     CDS             1679..2590
                     /locus_tag="PLQ70_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQE34018.1"
                     /translation="MNNFILIFVFLFLGVVLQKIKQFPTDFYKVLNKIVIYLCLPALA
                     LYYIPKIKLDSHLLFPIGVTWISFTTAILFFSLLGKRMGWSRKLTGCLILTAGFGNTS
                     FLGFPIIQALYGEEGLKMAIIVDQPGTFVVLSTLGVVVATLYSNGSSNGFQIAKKIIF
                     FPPFIAFLLACFMNVFQFDFHQYIQFGLLKIGSVMTPLAMLSVGLQLHFDKKSKHWKF
                     LRLGLFYKLILTPALIYLLYVVVFDQHSKMIQVSIMEGAMAPMISSSIIAATHGLKPR
                     LCSMMIGFGIPISFITLVFWYFVAGCI"
     gene            complement(2600..3181)
                     /gene="nudK"
                     /locus_tag="PLQ70_05065"
     CDS             complement(2600..3181)
                     /gene="nudK"
                     /locus_tag="PLQ70_05065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HQE34019.1"
                     /translation="MKDPKITIQKTELLSDNWYLLNKITFDYQEQNKPTETHIREVYD
                     RGNGAAILLYNPAEKTVILTRQFRLPSYLNKNQTGMMIEVCAGLLDQDHPEQCIIRET
                     EEETGYRISKVQKVMETYMSPGAVTEILYLFIGEYNASMKVHEGGGLDAEQEHIEVME
                     LPFEKAFEMIATGEIKDAKTVMLLQYAKINELV"
     gene            complement(3235..4506)
                     /locus_tag="PLQ70_05070"
     CDS             complement(3235..4506)
                     /locus_tag="PLQ70_05070"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQE34020.1"
                     /translation="MTADLLLGLQWGDEGKGKIVDVLTSKYDIIARFQGGPNAGHTLE
                     FDGIKHVLRTIPSGIFHKTAVNVIGNGVVIDPVVFQKEIEGLAKFNLDLKGKLIISRK
                     AHLILPTHRLLDAASEASKGKAKIGSTLKGIGPTYMDKTGRNGIRVGDIELEDFKERY
                     RSLANKHEEMIAFYDVDIQYNLDEMEKEFFESIEELKKIDFIDSEEYINQAQRAGKLI
                     LCEGAQGSLLDVDFGTYPFVTSSNTTAAGACTGLGIAPNKIKDVYGIFKAYVTRVGSG
                     PFPTELFDEDGETMARVGNEYGSVTGRKRRCGWLDLVALKYAVQINGVTQLMMMKGDV
                     LSGFPTLKVCTQYNYKGKAISHFPYNIETENVTPIYTELKGWEADLTGMTTYDELPKE
                     LKEYIEFIEKEIEVPIKIVSVGPDRKQTILK"
     gene            complement(4525..4992)
                     /locus_tag="PLQ70_05075"
     CDS             complement(4525..4992)
                     /locus_tag="PLQ70_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQE34021.1"
                     /translation="MTQISVDNSSNQEIVKNVFTLYLENKGHRKTPERYAILQEIYDS
                     EEHFDIENLYIKMKNKNYRVSRATLYNTIELLLESGLVRKHQFGQNQAHYEKSYFDKQ
                     HDHIIMTDTGEVIEFCDPRIQTIKKTIEDIFDIDITNHSLYLYGTKRKKEITN"
     gene            complement(5078..7297)
                     /locus_tag="PLQ70_05080"
     CDS             complement(5078..7297)
                     /locus_tag="PLQ70_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HQE34022.1"
                     /translation="MLEIDLEKENKAILQEYKELLRISYQTLSPEDKKLIRKAFDVAM
                     DAHKDQRRKSGEAYFFHPIAVAKIVASEIGLGATAIAAALMHDVVEDTPISVEDIEKM
                     FGPKVAQLVEGLTKISKVQKEMNVSMQAENFRKMLLTLHDDVRVILIKIADRLHNMQT
                     LDNMEDHKQIKIASETLYIYAPLAHRLGLYKIKNKLEDLGLKYTEPETYNDIVSKIKE
                     TKEEQDAYISDISEVLKKALDIEKLDYIIKGRPKSIYSIYRKMKAQGVSFDEVYDKFA
                     LRIVYKSSPHEEKFLAWKIYSIVTDHYRPSPSRLRDWISSPKTTGYEALHITVMGPKG
                     RWVEVQVRSERMDEIAEKGYAAHYKYKQGGSEESGLDIWLNLLKEALESSETNAVDFV
                     EDFKMNLYSKEIFVFTPKGEIKSLPKGATSLDFAFSIHSEVGIRTRGTRVNGKLVPLN
                     HELKSGDQIEIITSQYQKPTLNWLDYVTTSRAKTKIKNVLNENTKKIAEEGKEVLTRK
                     LKHLKITLSESVVNELVNYFKLKTSLDLFYRVGVGAIENQQLKDYAAQKSNTFINFFK
                     NKIKRNSSTASEDIHKQVLNSNYDMLVFGSDQDKLDYKLSTCCNPIPGDEVFGFITIN
                     EGIKVHKRDCPNAISLQSNYAYRIMQAKWIDSSQQEFKAILHITGMDTIGLTNKLTKV
                     ISDNMNVNIQSISLSGDAGIFNGQIAVIVQNNNILKRLIDNIKQIDGIDKVSREYNN"
     gene            complement(7377..8927)
                     /locus_tag="PLQ70_05085"
     CDS             complement(7377..8927)
                     /locus_tag="PLQ70_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQE34023.1"
                     /translation="MKKIISLVILLVSYHSFAQISEKQVDELVENTLKSFNVPGIAVA
                     IVKDGKVVLAKGYGLKSILTKEKVDANTLFGIASNSKAFTTAALAMLVDEKKLNWDDK
                     VIQYLPDFKMYNDYVTNEFTIRDLVTHRSGLGLGAGDLMIWPDGSDFKATDIIHNLKY
                     LKPVSAFRTKYDYDNLLYIVAGEVVAKVSGQSWCDFIENRIMKPLEMNHSAASFVRLK
                     DSTNIIAPHVPTDGKLKIISRYKNQTFDAAAGIYSSVNDLCKWMILQLQNGKYGSEKQ
                     FQLFSKKEQNEMWSLQTIIPANTRPPYNTHFSGYGLGWFLSDVKGYKQVTHTGGLEGI
                     VTQTTLFPELNLGIVVLTNQQSGAAFTAITNTIKDSYLDIPYTNYVEIYSKREKDNIT
                     EADKTTSEVWATIANNLKEKKKIDLKNYAGVFVDNWFGKIELSEKKGKLFFKSVRSPQ
                     LAGEVFFYKDTTFVVKWNTRSFNADAFLLFEMDENGKSNGLKMKPISDLTDFSYDFQD
                     LDFVRVKE"
     gene            complement(9007..9792)
                     /locus_tag="PLQ70_05090"
     CDS             complement(9007..9792)
                     /locus_tag="PLQ70_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQE34024.1"
                     /translation="MKQITSVQNPFIKSLVLLQEKAKARKQSGTFLIEGKREIEIAMK
                     GGYEMETILFLPELISETEARKLSKTAELIEISKEVYQKLAYRDTTEGILAVAKSKSL
                     QLLDLKLSKNPLILVAEAPEKPGNIGALLRTADAANLDAVIIANPKSDLYNPNIVRSS
                     VGCLFTNQIATGTTTEIIAFLKERNIDFYCATLQNSTSYHTQDYTTPTALVVGTEATG
                     LTQEWRDAATQNIIIPMQGEIDSMNVSVAAAILIFEAKRQRGF"
     gene            complement(9918..>10286)
                     /locus_tag="PLQ70_05095"
     CDS             complement(9918..>10286)
                     /locus_tag="PLQ70_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQE34025.1"
                     /translation="FSRLYTDIKKNINQRSLIVLYTNFETMDSLHRQLPYLKGIAKNH
                     LLVVVFFQNTELNELISKKAENIQEVYDKVIAEKFSFEKRLIVNELKKYGIYSVLTQP
                     ENLTLDTINKYLEIKARGIL"
BASE COUNT         2861 a         1856 c         1794 g         3775 t
ORIGIN      
        1 ttagatttca gatattagat ttcagatttt cacgaatcta aaatctaaat tcaaaaatta
       61 taaaatggaa ttaccaaaat ttttactagg cgataacact gattttccag atgatatttt
      121 catcattcat ttggattatc caagattcat catcaatcta aaagacgatg aagtagaatt
      181 tatggaagaa gctgaagatc ttgatgaagc tgaattaaat gccgaaatgg aaggtttgat
      241 tgaacaagcc aatgcctttt atgaccgaga aatggaacgt tacgaaaaag aataatattt
      301 tttcaaaaat tttttccata acaaaaagag ctgtaactga tgctatttag tcggagatta
      361 ttttaaattt ttgaaatagt tttccaatac gaattgagcg gctgcaaaag gtgaaatttc
      421 atcattttgt accgcttttt tagtagattc caaaactttc tggatttcag gattgttgta
      481 gaaattagtt ttcaattgtt cattaatggt ttcaagtaac caatattgat tttgtgctgc
      541 ccgtttttca aagaaataag tattcccttt agtgatttcg ataaattgct cgattgtttg
      601 ccaaatttcc ggaattcctt ctcgtgttat cgaactacaa gttgaggttg ttggaatcca
      661 acccgatttt ttggcaggaa ataaatgcaa agcacgttca aattcagttt ttgctaattt
      721 ggactttttg atgttatcgc catcacattt attgatgaca atagcatcga ccatttccat
      781 aatacctcgt ttgatgcctt gtaattcatc gccagcgcct gaaattttca aaagcaaaaa
      841 gaaatcgacc atactatgaa ctgcagtttc gctttgtcca acaccaacag tttcaatgag
      901 aatagtgtca aaaccagcag cttcacatag cataatggtt tctctagttt ttcgagccac
      961 accgccaagc gtttctcctg aagacgaagg tctgatatag gcattagggt ctttaattaa
     1021 ttcttccatt cgggttttat cgcctagaat acttccgtgc gaaatggtgc tgcttgggtc
     1081 aacggcaagc acggctactt ttttgcctag accagttaaa tgttttccaa atgcttcaat
     1141 aaaagtactt tttccaacac caggaactcc cgttattcct attcttactg atttattggc
     1201 gtgaggtaag caagcgttta gtacttcatt ggctttcgcc aaatggttgg catttgtact
     1261 ttcaattaga gttacggctc ggcttaaatc tgaaatattt cctgctaaaa ctccattgat
     1321 taattcctga gttgtgcgtt gtttttttct aagtagttga atttgctcca cagctttcga
     1381 actgataatt tctggaggtg aaattccatc tttttcgtgt aaactgcttg gatgttgatt
     1441 tttattgtcc aaaatgcttt gtttgagtag taaaagtagt gaaaacaaaa acagtttcaa
     1501 aaagggattg ctctttgaat ctggtttgtt ataaatggtt gtattttgta gttatcactt
     1561 caaggctaaa ctctggtagc aaataatttc ttttatctgg ggtaaataaa agattaaaaa
     1621 tagatttcca cattttcaaa ataaatttta gtggtatttt tgcagtcaat ctactactat
     1681 gaacaacttt attctaattt ttgttttttt atttcttgga gttgttttgc aaaagataaa
     1741 gcaatttcca acagattttt ataaagtatt aaataagatt gtaatttatc tttgtcttcc
     1801 agctttggca ctttattata ttccaaaaat caaattagat tcgcatttgt tgtttcccat
     1861 tggtgtcact tggattagtt ttaccacagc aattttgttt tttagtttgt tagggaaaag
     1921 aatgggatgg tccagaaaat tgacgggatg tttgatttta acggcgggtt ttggcaatac
     1981 ttccttcttg ggttttccaa ttattcaagc tttgtatgga gaagaaggtc tgaaaatggc
     2041 tataattgtt gatcagccgg gaacttttgt tgtgctttcc actttgggag ttgttgtagc
     2101 tactttgtat tctaacggaa gttcaaatgg atttcaaatt gctaaaaaaa ttatcttttt
     2161 tcctccgttt atcgcttttt tgttggcgtg ttttatgaat gtttttcaat ttgattttca
     2221 tcaatacatt cagtttggtt tactgaaaat tggaagtgtg atgacgccat tagcgatgtt
     2281 atccgttgga ctccaacttc attttgataa aaagagcaag cattggaaat tcttaaggtt
     2341 aggacttttt tataaattga ttttgacacc agcactaatt tatttgttgt atgtagttgt
     2401 ttttgaccaa cattcaaaaa tgatacaagt ttcaattatg gaaggagcga tggcaccaat
     2461 gatttcgtcc agtattattg ctgctactca cggtttgaaa ccccgattgt gcagtatgat
     2521 gattggtttt ggaattccaa tttcttttat caccttggtt ttttggtatt ttgtggcagg
     2581 ttgtatttga tgtttttaat cataccaact cattaatctt cgcatactgc aacaacatca
     2641 cggtcttagc atctttaatt tcaccagttg caatcatttc aaaagctttt tcaaaaggaa
     2701 gttccatcac ttcaatatgt tcttgttctg catcgagtcc gccaccttcg tgtactttca
     2761 ttgaagcatt gtattcacca ataaataaat acaaaatttc ggttacggcg ccaggtgaca
     2821 tataggtttc catcactttt tgaacctttg aaattcgata tccagtttct tcttcggttt
     2881 ctcgaataat acattgctct ggatgatctt ggtctaaaag tccagcacaa acttcaatca
     2941 tcattccagt ttggttttta tttaaatagg atggcaatcg aaactgacgt gttaaaatca
     3001 cggttttttc agcagggtta tatagtagaa tggcggctcc gtttcctcga tcatatactt
     3061 ctcttatgtg agtttctgtc ggtttatttt gttcttgata atcgaaagtt attttattta
     3121 ataaatacca attgtctgaa agtaattcgg ttttttgaat ggttattttg ggatctttca
     3181 tcggattttt aatgtttaaa aaaaaacgtc ccgattaatc gggacgttaa gttattattt
     3241 tagaatggtt tgttttctgt ctggtccaac agaaacaatt tttattggca cttcaatttc
     3301 cttttcgata aattcgatgt actcttttaa ctcttttggc aactcatcat aagtcgtcat
     3361 tccagttaaa tcagcttccc atccttttaa ttcggtatag attggagtta cgttttcagt
     3421 ttcgatattg tatgggaaat gtgaaatcgc ttttcctttg taattgtatt gagtacatac
     3481 tttcaaggtt gggaatccag aaagaacgtc tcctttcatc atcatcaatt gagtaactcc
     3541 attgatttga actgcatatt tcaaagccac taaatccaac catccacaac gacgttttct
     3601 gccagttact gaaccatatt cattaccaac tcttgccatt gtttctccgt cttcgtcaaa
     3661 aagttctgtt gggaacggac cacttcctac acgagttaca taggctttga aaattccgta
     3721 cacatctttt attttgtttg gagcaattcc caaaccagta caagcccctg cagcagttgt
     3781 gtttgatgaa gttacaaatg gatatgttcc aaaatcaaca tctagtaacg aaccttgagc
     3841 gccttcgcat aaaattaact ttccagctct ttgggcttga ttgatgtatt cttcgctgtc
     3901 tataaaatct atttttttca attcttcgat agattcaaag aattcttttt ccatttcatc
     3961 taagttgtac tgaatatcaa catcataaaa tgctatcatt tcttcgtgct tgttggccaa
     4021 tgagcggtat ctttctttga aatcttctaa ttcgatatcg ccaacacgga ttccatttct
     4081 tccagttttg tccatatacg ttgggccaat tcctttaaga gtagaaccaa tttttgcttt
     4141 tccttttgaa gcttcagaag cggcatcaag caaacggtgg gttggtaaaa ttaaatgtgc
     4201 ttttctagaa atgattaatt tgccttttag gtctaaatta aattttgcta atccttcaat
     4261 ttctttttga aatacaacag gatcaattac tactccattt ccaataacat ttactgcggt
     4321 tttgtgaaaa attccagaag gaatggttct caaaacgtgt ttaattccgt caaattctaa
     4381 tgtatgtcct gcatttggac cgccttgaaa gcgggcaata atgtcatatt ttgacgtaag
     4441 tacgtcaaca attttccctt ttccttcatc tccccattgt aatcctagta ataaatctgc
     4501 ggtcattcta atttttattt gtagttagtt tgttatttct ttttttcgtt ttgttccgta
     4561 tagatatagg gaatgatttg taatgtctat atcaaaaata tcttcgatcg tttttttgat
     4621 agtttgaatt ctaggatcac aaaattcgat aacttcgcca gtatcggtca ttattatatg
     4681 gtcgtgttgc ttgtcgaaat aggatttttc gtaatgagcc tgattttgtc caaattgatg
     4741 tttgcgcacc aaacctgatt ctaataaaag ttctatggtg ttgtaaagtg tagctcgact
     4801 cacacggtag tttttatttt tcattttgat atacaaattt tctatatcaa aatgttcttc
     4861 gctatcatat atttcttgga gtatagcata gcgttcaggc gttttgcgat gtcctttatt
     4921 ttcaagatag agtgtaaaaa cattttttac aatttcttga ttactactat tatcaactga
     4981 aatctgtgtc atatctgcaa agataaattt tattttttag tttagaagtt taaaaggtta
     5041 gaataaacca agaactaaac atatttctct ctttttgtta attattgtat tcacgactca
     5101 ctttatcaat accgtcaatt tgcttgatat tatcgattaa tcttttcaaa atgttattgt
     5161 tttgaacgat tacggctatt tgtccgttga aaattcccgc atcgccactc aaggaaatac
     5221 tttggatatt cacattcata ttatcagaaa ttactttggt caatttattg gttaatccaa
     5281 tggtgtccat tcctgtaata tgcaatatcg ctttaaattc ttgctgtgaa gagtcgatcc
     5341 acttggcttg cataattcga tacgcataat tcgattgaag acttatggca ttaggacaat
     5401 ctcttttgtg aactttaatt ccttcattta ttgtaatgaa tccaaaaact tcatcgcctg
     5461 gaatgggatt acaacacgta gataatttat aatctaattt gtcttgatct gatccaaaaa
     5521 ccagcatgtc ataattgcta ttgagcactt gtttgtgaat gtcttcgctt gctgtgcttg
     5581 aatttctctt gattttattc ttgaaaaaat tgataaaagt gttgcttttt tgagccgcgt
     5641 aatcttttag ttgttgattt tcaattgcgc caacacccac gcgataaaac aaatctaaac
     5701 ttgtttttaa tttaaaataa ttgaccaatt cattgactac cgattcgctc aaagtgattt
     5761 ttaagtgttt gagttttcgg gtaagtactt cttttccttc ttcggctatt tttttggtat
     5821 tttcgtttaa aacattttta attttagttt ttgccctaga agtcgttaca taatcaagcc
     5881 agttcaaagt gggtttttga tattgagagg taataatttc aatttgatcg ccacttttta
     5941 attcgtgatt caatggaact aatttcccgt tgacacgtgt tcctcgtgta cgaattccaa
     6001 cttcggagtg gatgctgaaa gcaaaatcta atgatgtagc cccttttggc agtgatttta
     6061 tttctccttt tggagtaaac acaaagattt cttttgaata taaattcatt ttaaaatctt
     6121 ctacaaaatc taccgcattg gtttctgaac tttctaaggc ttcttttaaa aggttaagcc
     6181 aaatgtctaa accactttct tcagatccgc cttgtttgta tttgtaatgg gctgcatatc
     6241 ctttttcggc aatttcgtcc attcgttcgc ttcttacttg cacttcaacc cagcgacctt
     6301 ttggtcccat aactgtgatg tgcaaggctt cataacccgt ggtttttgga gaagaaatcc
     6361 aatctcttaa tcggctagga cttggtctgt aatgatcggt tacgatagaa taaattttcc
     6421 aagcaagaaa tttttcctcg tgaggagacg atttgtaaac aattcgcaaa gcaaatttgt
     6481 catacacttc atcaaaacta actccttgag ccttcatttt tctataaata gaataaatag
     6541 atttaggtct tcctttgatg atataatcga gtttttctat atccaatgct ttttttagca
     6601 cttccgatat gtcacttata taagcatctt gttcttcttt ggtttccttt attttgctaa
     6661 caatgtcgtt gtaagtttca ggttcagtgt attttaaacc taaatcttcg agtttgtttt
     6721 ttattttata caaacccaat cggtgagcca aaggggcata aatgtataaa gtttcagaag
     6781 cgattttaat ttgcttgtgg tcttccatat tgtctaaagt ttgcatatta tgcaatctgt
     6841 cggcaatttt tatcagaatt actcgcacgt cgtcgtgaag agtcaacagc atttttcgaa
     6901 aattctcggc ttgcatcgac acattcattt ccttttgcac tttcgaaatt ttggtcaatc
     6961 cttccaccag ttgagctact ttgggaccga acattttttc gatatcttcc actgatattg
     7021 gggtgtcttc tacaacatcg tgcatcaagg cagccgcgat ggcagtggct cccaaaccaa
     7081 tttccgaagc tacaattttg gccacagcaa taggatggaa aaagtaagct tctcccgatt
     7141 ttctacgctg atctttgtgc gcatccatag ctacatcaaa agctttacga atgagtttct
     7201 tgtcttcggg gcttaaagtt tggtaactaa ttcgaagtaa ctctttgtat tcctgtaata
     7261 tcgctttgtt ttccttttct aaatctattt ctaacataaa aaaatggttg atttcttaaa
     7321 aataggcata actaatgaga tatgcaaacc gggattaaaa tttatcgtgt ttaaaattat
     7381 tctttaactc taacaaaatc taaatcttga aaatcataac taaaatcggt taagtctgaa
     7441 atcggtttca tctttaaacc gtttgatttt ccgttttcat ccatttcaaa tagtaagaac
     7501 gcatcggcat tgaagcttcg tgtattccat tttacaacaa aagtagtgtc tttgtaaaag
     7561 aaaacttcgc ccgcaagttg aggagaacga accgatttga aaaataattt tccttttttt
     7621 tctgaaagct caattttccc aaaccaattg tcaacaaaga cacctgcata attttttagg
     7681 tcaattttct ttttttcttt tagattattg gcaattgttg cccaaacttc agaggttgtt
     7741 ttatccgctt cggtgatatt gtctttttct cttttgctat aaatttcgac ataattggtg
     7801 tacggaatgt cgagataact gtctttgatg gtattggtaa tggccgtaaa agctgctccc
     7861 gattgctgat tagtcaaaac tacaattccc aggttcagtt ctggaaataa ggtagtttga
     7921 gtaacaattc cttcgagtcc gcccgtgtga gtgacttgtt tgtaaccttt aacatcgctc
     7981 aaaaaccaac ccaatccata tccgctgaaa tgagtattat aaggaggtct tgtattggcg
     8041 ggaattatcg tttgaaggct ccacatttcg ttttgctctt tttttgaaaa taattgaaat
     8101 tgcttttctg agccatattt cccgttttgc aattgcagaa tcatccattt gcacaaatcg
     8161 tttacactcg aataaattcc ggcagccgca tcgaaagttt ggtttttata acgtgaaatt
     8221 atttttagtt tcccatctgt cggaacgtgt ggcgcaataa tattggtgct atcttttaat
     8281 cgcacaaaag aagctgcact atgattcatt tccaatggtt tcataatgcg gttttcgata
     8341 aaatcacacc aagattgccc gctgactttc gcaacaactt cgccagccac gatgtataat
     8401 aaattgtcgt aatcgtactt ggtcctaaaa gccgaaacag gttttaaata cttcaaattg
     8461 tggatgatgt ccgttgcttt aaaatcgctt ccgtctggcc aaatcatcaa atcgcctgcg
     8521 ccaagtccca atccactgcg gtgcgtcacc aaatcccgaa tcgtaaattc attggttaca
     8581 tagtcgttgt acattttgaa atcaggaaga tattgaatca ccttatcgtc ccagttgagc
     8641 tttttttcat cgactaacat cgccaaagct gcggtggtaa aagccttgct gtttgaagca
     8701 atcccgaaca aggtgttggc atccactttc tctttggtca ggatcgattt tagtccgtag
     8761 cctttagcaa gaaccacttt tccgtcctta acaatagcca cagcaattcc aggtacgtta
     8821 aagcttttta gcgtgttttc gactaattca tcaacttgtt tttcgctaat ttgtgcgaaa
     8881 gaatgataac taacaagcaa tattactaag gaaattattt ttttcatttt cataattttt
     8941 tatttctaaa atcactaatc tatttcaata aatctgaatt caaaatcgtt aatctacaat
     9001 cgaaaatcaa aatccccttt gtcttttggc ttcgaaaatc aagatggcag cggcaaccga
     9061 aacgttcata ctgtcaattt cgccttgcat tgggatgatg atgttttgtg tggcggcatc
     9121 tcgccattct tgggttaaac ctgtggcttc agtgccaacc accaaagccg ttggagtggt
     9181 ataatcttgg gtgtggtagg aagttgaatt ttgtaaagta gcacagtaaa aatcgatgtt
     9241 tcgttctttc aaaaatgcaa tgatttcggt cgtggttccc gtggcaattt ggttggtaaa
     9301 caaacacccc acgctggagc gaacaatatt cgggttgtat aaatcacttt ttgggttggc
     9361 tataatcacg gcatcgagat tggcggcatc ggcagttctc aataaagcac caatatttcc
     9421 tggcttttcg ggagcttcgg ccacgagaat caaaggattt ttggataatt tcaaatccaa
     9481 caattgcagg gatttcgatt tggccacggc caaaatgcct tcggtggtat cgcgatacgc
     9541 cagtttttga tagacttctt tcgaaatttc gattaattca gctgttttgg aaagttttct
     9601 ggcttcggtt tccgagatta attcgggcaa aaacaaaatc gtttccattt cgtaaccgcc
     9661 tttcatcgca atttcgattt ctcttttgcc ttcaatcaag aaagtgccgg attgtttgcg
     9721 ggcttttgct ttttcctgca acagcaccaa ggatttgatg aatggatttt gaacggaagt
     9781 gatttgtttc attttttgaa ggttctgaga gactaagttg caaaggtact caggtttttt
     9841 tcttttgcca cagatttcac agattttcac agatttttcg gtgctatgtt tcaaatctga
     9901 aatcgttaat ctaaaatcta taaaatccct ctcgccttga tttccaaata tttgttgatg
     9961 gtgtccaaag tcaggttttc gggttgggtg aggacggaat aaatgccgta tttcttgagt
    10021 tcgttgacga ttaatcgttt ttcgaacgaa aatttctctg caatcacctt atcataaact
    10081 tcttggatgt tttcggcttt tttgctgatg agttcgttga gttcggtatt ttggaagaaa
    10141 accacgacca ataaatggtt tttggcaatg cctttcaaat acggcaattg gcgatgcaaa
    10201 ctgtccatcg tttcgaaatt ggtgtacaaa acaatcaaac tccgttggtt gatatttttc
    10261 ttgatgtcgg tgtacaaacg gctgaa
//
LOCUS       DAOSNN010000069        10244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6538_length_10244_cov_5.604377, whole genome shotgun sequence.
ACCESSION   DAOSNN010000069 DAOSNN010000000
VERSION     DAOSNN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6538_length_10244_cov_5.604377"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLQ70_05100"
     CDS             complement(<1..468)
                     /locus_tag="PLQ70_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34026.1"
                     /translation="MFELYKKRDLSANFSDTTAFFKTFGKHYFKNYFIINGIFLMILV
                     VLIYFISKVYMELIFSGITNMQNNPNYLMAYFNNNMALIVGGFVITFLLIVILSMLNI
                     SYPVIYMGLLEKNNSTNFQIQEIISGLKSNIGKMIIFLLGSIFIIMPLAAIVFG"
     gene            complement(468..1433)
                     /locus_tag="PLQ70_05105"
     CDS             complement(468..1433)
                     /locus_tag="PLQ70_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HQE34027.1"
                     /translation="MREIAFIKTNKDKWLEFEQAIFGKAKKNPDEMANLYIQLMNDLS
                     YAQTYYAKSKTVVYLNYLASQIYQKIYKTKRTESNRLVYFFKTEVPLILYDYRRYLLY
                     AFVLFFVTVLIGAVSARYDADFVRLILSDGYVNKTLENIKDGNPVAIYKSGSNWGSFI
                     GITFNNLKVGMMCYIFGIFGGIGTFLISLQNAIMLGSFQYFFFQQGVFWKSVRGIWIH
                     GAMEIFGIVIETAAGYILGASILFPKTYSRMNSFKIGFKNSFKIFLSTVPFTIAAGFL
                     EGFITRYSIDMPNWLSSFIILFTLAIISFYYLIYPFIVHKKITTI"
     gene            1454..2197
                     /locus_tag="PLQ70_05110"
     CDS             1454..2197
                     /locus_tag="PLQ70_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQE34028.1"
                     /translation="MSELSINTTQNVTINFKSASVGERMLASLLDFLIKVAYVIVVAY
                     VFFDLLGISIIMDKLDFWSQMAIVILFFFPVMIYSVTLESIFEGQTIGKKLIKIKVVK
                     IDGYQAGFGDYLIRWLFRLVENNLMGGLIGLVAMLVSNKTQRMGDVAAGTAVITLKNN
                     ISINNTILEEIGDAYQPIYPLVIKLSDNDMRIIKDTFLIAEKKNDTETILKLSRKIEA
                     VTGIKNQSGNQIDFLRTVLKDYNFYTQNM"
     gene            2259..3236
                     /locus_tag="PLQ70_05115"
     CDS             2259..3236
                     /locus_tag="PLQ70_05115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE34029.1"
                     /translation="MKTFSVKQYQKNDFENWNAFIGQAKNATFLFHRDFMEYHNDRFE
                     DYSLIVLDGEKWVAVLPANVVGNSVFSHQGLTYGGLVYTEKLKLASVIEIFKSVLLFL
                     NENKIVKLHLKLIPSIYNQKPAEELNYALFLTEAQLIRRDTLAVIDFSQQNSLSKLRK
                     RSIQKGISNDFSIKEVNDFEKFWDEILIPNLEQKHQVKPVHSLEEITKLKVLFPKNIR
                     QFNVYEGGTIIAGTTIFETSTVAHCQYISGREDKNELGGLDVLFQHLISDIFKHKRFF
                     DFGISNENQGRKLNNGLSYWKESFGASTIVQDFYEVETVNYKLLGNVLK"
     gene            3233..4333
                     /locus_tag="PLQ70_05120"
     CDS             3233..4333
                     /locus_tag="PLQ70_05120"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQE34030.1"
                     /translation="MIKFLDLKKINQRYEAEFQDKLKSVLEGGWYILGNEVAAFESDF
                     ANYCGTKHCIGVGNGFDALVLIFKGYIQLGKLQKGDEVIVPANTYIASILAIMQADLV
                     PVLVEPKLETYNINPDLILEKITAKTKAILVVHLYGQLAEMDAINEIAQLKKMIVVED
                     AAQAHGATSSQQLAVSSEQSAIAYSFYPGKNLGALGDGGAVVTNDAELAKVIQSLRNY
                     GSEAKYQNKFIGVNSRLDELQAAFLNVKLPKLDAENSIRKSIAKRYLTEIKNNKITLP
                     FWDLSDNHVFHLFVIRTPKRNELQQYLLENGIQTMIHYPIPPHKQKAFESWSNLSFPI
                     TEKMHNEVLSLPISPVLTMDEVDFVISILNQY"
     gene            4335..5609
                     /locus_tag="PLQ70_05125"
     CDS             4335..5609
                     /locus_tag="PLQ70_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen translocase"
                     /protein_id="HQE34031.1"
                     /translation="MEFIKKITQTNLFKITSLNSVSVLIKIGIGLVTSKLLAVFVGPS
                     GMALVGNLRNFSTSLESISTLGFQNGIVKYVAESKEDKHQLQKTISTIFISLLVIAML
                     LSSILYFFAAFWNQQIFGTNYEYEIVIRAMALALPWFAIAVFLLAVINGLGKFKEVIW
                     INIVGNIIGLLVSIGMILQYKTLGALLSIVITPALLFFVTYYFISKEFHFSKFIQYKS
                     YDLHVIKNLSSYSLMALVSSVLGPLVFLAIRKNVIVVLGIQEAGFWETMSRISTYYMM
                     FVSTILTVYFLPKLAVAKDNIETKKVFWSFYKNILPLFAIGLVLIYFSRTLLIKLLFA
                     SEFLPVTDLFLWQLFGDVLKVAAMILGYQFYAKKMTLAFIISEMASLAVLYFSSIYLI
                     AICGIQGIVMAQALDNFIYFLVLAIYFRKSLF"
     gene            5700..5885
                     /locus_tag="PLQ70_05130"
     CDS             5700..5885
                     /locus_tag="PLQ70_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34032.1"
                     /translation="MIPLYFDPGTGALIVQFLAAAVAGILLFYRTVLHKIKSIFGIKN
                     KEEDDFMDETDNSEDTK"
     gene            5886..7256
                     /locus_tag="PLQ70_05135"
     CDS             5886..7256
                     /locus_tag="PLQ70_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQE34033.1"
                     /translation="MESKRNTSSFRDPSGYVFIEDDSVKRVVNHIYFEQYNQLTSSGF
                     YQLLFDKKYLVSHQEVSRTDSEIIIEASKIPFINYPYEWSFLQYKHAALLTLKIQKLC
                     LENNFTLKDASAFNITFHEGKPIFIDTLSFDFYQENNPWLAYKQFVMHFLGPLVLTKY
                     FGQDHLKTLALHLEGISLQKLSNLLPLKSYWSPILLTNIHLLAKYDKKYESDKKKVNN
                     NLSKASQIKLLDGLYDYILNLSVNEKTEWDHYYNQINYNEVAYQVKKEFVKDWFSSIK
                     GKSLIDIGGNDGTFSRELKELADFMIVADVDANAVEQNYKQILKNKEKSILPIVVDVL
                     NPVANYGFNNQERFSFIDRVEEANLDAGLALAVIHHITLSGNIPFSLSAQFFSKMAPN
                     LLIEFPTRADSWVQFLLDSKREFKSHFDFYNEENFENEYAVYFEIVHKVKIDSSERIL
                     YSLKRR"
     gene            7257..8726
                     /locus_tag="PLQ70_05140"
     CDS             7257..8726
                     /locus_tag="PLQ70_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34034.1"
                     /translation="MNLKIKDKLNYFLKNDEDYPILSGFSVGFYAMVFYFSNNFDLVN
                     SWEQTLFFLFYYIIVSVGVVFVISKIIEKSKYNSFVSQAIFVSMLLLLPIYLFGISNI
                     LDSNKKMGLVLILILSLFIFKIKNYKYCIVLLFLMSVIPVIKLGKIIYLNYTNSTEWK
                     LQPDAILKAEFKKKPNIYYIQPDGYAGEVNLKGNLYRYDNSAFDTFLKEKKFTLYPNY
                     RSNYESTLFSNSSCFFMKHHFSNEFSKFKYARDLIIGENPVLKILKNNNYKTFFITER
                     PYLLMNRPEVFYDYCNFKVGELPYLSDGWSDFREITNTIKTQIINNKDTNNFFFIEKF
                     KPGHIATYKFAGTNKEKERIKYIESIEEANQWLQQIIVFIEAKDPGAMIIIGADHGGF
                     VGFDYALEAQKKVTDSMLIQSIFGAKLAIKWNNVQHSEYDSKLKTPVNLFRTVFSFLS
                     EDKNLLNHLQPDASYNCYDPTDFTKVYKAIDENGSTAKF"
     gene            8792..10162
                     /locus_tag="PLQ70_05145"
     CDS             8792..10162
                     /locus_tag="PLQ70_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34035.1"
                     /translation="MLSKILNSTLLAKINSKEIKILVVFGLLYRIIFSVFYFHITIYP
                     DSEGYLFLSQKILNFNLSHYNGERSLGYPLLISFAFGSTIITLLYQFILGIITSIYWY
                     KTLINLRFSSKKALCVTLFLQSFINVYFYETAILVESLSLFFISVIVFMLTDNYFENR
                     SLKNDFLIGFIIAYLVAIKPFYIYIPFVIYLFVILKNHNLKRLIFNKIVILILPLIMF
                     FGWSYVNKINTGHFVSTTFFGLNLSQNCVYFAEKAPKEFDWIAKPYVKYREMAIKQDK
                     DVAMSIWFARKHGAFASYNLKLCDLSSELGDFAKETIRNNPIDYLKQVFTRSWFDFWK
                     PSIYWNFDKFNFEYAKKIVLLIWYIQVPITVFFKFTFVLLIPILSYFAFKRREISIEF
                     IVSIIVFSGSILQGLATYGTNNRFSFPFEFIMILVVISFFKREIYFSKHLNTFRQFLN
                     NYALFR"
BASE COUNT         3435 a         1397 c         1747 g         3665 t
ORIGIN      
        1 accaaaaacg attgcagcta aaggcattat gataaatatg cttcccaata agaaaattat
       61 cattttccca atgtttgatt ttaagcccga aatgatttct tggatttgaa aattagtact
      121 gttatttttt tctaaaagcc ccatataaat tacaggataa ctaatattga gcatcgataa
      181 aatgacaatt aacaaaaagg tgatgacgaa acctccaaca atgagcgcca tattgttgtt
      241 gaaataagcc atcaggtagt tgggattgtt ctgcatattg gttattccgg agaaaatcaa
      301 ctccatatag actttggaaa taaaataaat caaaaccacc aaaatcatca ggaatatgcc
      361 gttgatgatg aaataatttt tgaaataatg ctttccaaaa gttttaaaaa aagcagtcgt
      421 atcagagaaa ttggcgctta aatcacgctt tttgtagagt tcgaacatta gattgtagtt
      481 atttttttgt gaacgataaa cggataaatc aggtaataaa aagagatgat ggccaaggta
      541 aacaaaataa taaaactgct cagccagttg ggcatatcaa tagaatagcg agtgataaaa
      601 ccttcgagaa aacccgctgc aatcgtgaat ggaacggtac tcaaaaatat tttgaaactg
      661 tttttgaagc caattttaaa agaattcatt ctcgaatagg ttttaggaaa taggatagaa
      721 gcacccaaaa tataacccgc agcagtttct atcacgattc cgaaaatttc cattgcaccg
      781 tgaatccaaa taccgcgaac gcttttccag aaaacccctt gttggaagaa gaaatattga
      841 aaagaaccca acataatggc attttgcaac gaaatcaaga aagtgccaat gcctccaaaa
      901 attccaaaaa tgtagcacat cattcctact tttaggttat tgaaagtaat gccaataaaa
      961 ctgccccaat tgcttcccga tttgtaaata gccactggat ttccgtcctt aatgttctct
     1021 agcgttttat tcacatagcc gtcgctaaga atcaatcgaa caaaatcggc atcgtatctg
     1081 gcagaaactg ctccgatgag aacagttaca aaaaacagca caaaagcgta aagtaaatac
     1141 ctgcggtaat cgtaaagaat taatggtact tctgttttga agaaatagac caatcgattg
     1201 ctttcggttc gtttggtctt gtaaatcttt tgataaatct gtgaagctaa atagtttagg
     1261 taaactaccg ttttgctttt ggcataataa gtttgggcat aggacaaatc attcattaat
     1321 tgaatgtata aatttgccat ttcatcagga ttttttttag ctttaccaaa aatagcttgt
     1381 tcaaattcaa gccatttgtc cttattcgtt tttataaatg caatttctct catcgagtgc
     1441 taaaataaga aatatgtcag aattatctat aaatacaaca caaaatgtta caataaattt
     1501 taaatcagct tctgtcggcg agagaatgtt ggctagtttg ctggattttc tcattaaagt
     1561 ggcttatgtt attgtagttg cttatgtttt tttcgactta ttagggatct caataattat
     1621 ggataagtta gatttttggt ctcaaatggc gatagtaatt ctgttttttt ttccagtaat
     1681 gatttattcg gtaacactcg aaagtatttt tgaaggacaa actattggaa aaaaattaat
     1741 caaaataaaa gtggtgaaaa ttgacggtta ccaagccggt tttggtgatt atcttattcg
     1801 atggcttttt cgtcttgtcg aaaataattt gatgggtgga ttaatcggat tggttgcgat
     1861 gttagttagc aacaaaacgc agcgaatggg agatgttgct gccggtacag ctgtcataac
     1921 gctgaaaaat aatatttcta tcaataatac gattctcgag gaaataggtg atgcatatca
     1981 gcccatttat cctttggtta tcaagctttc ggataatgat atgcgaatta ttaaagacac
     2041 ttttttaatc gccgaaaaga aaaacgacac tgaaaccatt ttgaaattat ctagaaaaat
     2101 cgaagctgtt acaggtataa aaaatcaatc gggcaatcaa atagattttt taagaactgt
     2161 tttgaaagat tataattttt atactcaaaa catgtaaaaa gaagtttttt agaacttcaa
     2221 tttaaattgt ttaatttcac aatgtctaaa tcaaaatagt gaaaacattc tctgtaaaac
     2281 aataccaaaa aaacgatttt gaaaattgga atgcttttat tggccaagcc aaaaatgcta
     2341 ctttcctttt tcatcgtgat tttatggaat accacaacga taggtttgag gattattctt
     2401 tgattgtttt agatggcgaa aaatgggtag cggtacttcc tgccaatgtg gttggcaatt
     2461 cagttttctc gcatcaagga ttgacttatg gcggattagt ttatactgaa aaactaaaat
     2521 tagcctctgt aattgaaata ttcaaaagtg ttttattgtt tttgaatgaa aataaaatcg
     2581 tgaaattgca tttaaaatta atcccatcta tttataatca aaagccagcc gaagaattga
     2641 attatgcttt gtttttgaca gaagcccaac taattcgaag agatactttg gcagttattg
     2701 atttttcgca gcaaaatagt ttgtcaaaat tgagaaaaag aagcatacaa aaagggattt
     2761 cgaatgattt ttcgataaaa gaagtaaatg attttgagaa attttgggat gagatattaa
     2821 ttccaaattt agaacaaaaa caccaagtaa aacctgttca tagtttggaa gaaattacca
     2881 aattaaaagt tttgtttcct aaaaatatta ggcaatttaa tgtttatgaa ggaggaacta
     2941 ttattgctgg aactaccatt tttgaaactt caacggtggc tcattgtcag tatatttcag
     3001 gtagagaaga taaaaacgaa ttgggaggat tggatgtatt atttcaacat ttgatttcag
     3061 atattttcaa acacaaacgc ttttttgact ttggaatttc taacgaaaat caaggacgaa
     3121 aacttaataa tggtttgtcg tattggaaag aaagttttgg tgcaagtact attgttcagg
     3181 atttttatga agtggaaaca gtaaattata aattattagg caacgtttta aaatgataaa
     3241 attcctcgat ttaaaaaaaa taaaccaacg gtatgaagct gaatttcaag ataaattgaa
     3301 atcggtttta gaaggtggtt ggtatatttt gggtaatgaa gtcgctgcat ttgaaagtga
     3361 ttttgccaac tattgcggga cgaaacattg cataggagta ggcaatggtt ttgatgcttt
     3421 ggttttgata ttcaaaggct atatccaatt ggggaaattg caaaaaggag acgaagtgat
     3481 tgttcctgcc aatacttata tcgccagtat tttggctatt atgcaggccg atttggtgcc
     3541 tgttttggtc gaacccaagt tggaaactta taacattaat cctgatttga ttcttgaaaa
     3601 aatcacagcc aaaactaagg ctattctagt ggttcatttg tatggacagt tggccgaaat
     3661 ggatgcaatt aatgaaattg ctcaactaaa aaaaatgatt gttgttgaag acgccgctca
     3721 agcgcacgga gcaactagca gtcagcagtt ggcagtgagc agtgagcagt cagcaatcgc
     3781 ttacagtttt tacccaggaa aaaatttagg tgctttgggc gatggaggag ctgttgttac
     3841 taatgatgct gaattagcaa aagttattca gtcactaaga aattatggtt cggaagccaa
     3901 atatcaaaat aaatttattg gtgtaaactc cagattagat gaattgcaag ccgctttttt
     3961 aaacgtaaaa ttacctaaat tagatgctga aaattcgatt cgaaaatcta ttgctaaacg
     4021 ctatttgact gaaataaaaa acaataaaat tactttgcca ttttgggatt tgtccgacaa
     4081 tcatgtgttt catttgtttg tcattcgaac cccaaaaagg aatgaattgc aacagtattt
     4141 attagaaaac ggcattcaaa cgatgattca ttatccgatt ccgccacaca agcaaaaagc
     4201 ttttgaatct tggagtaatt tgtcttttcc aattaccgaa aaaatgcata atgaagtctt
     4261 gagtttgccc ataagtccag tattgactat ggatgaggtg gattttgtaa tttcaatttt
     4321 aaatcagtat taaattggaa tttatcaaaa aaattaccca aactaattta tttaagatca
     4381 cttctttaaa tagtgtaagt gttctaatca aaattggtat tggattggta acttcaaaat
     4441 tattggctgt ttttgtgggt ccaagtggaa tggccttggt tgggaattta agaaattttt
     4501 caacttcatt agaaagtatt tcaactcttg gttttcaaaa cggaattgtc aaatatgttg
     4561 cagaaagcaa agaagataag catcaacttc agaaaacaat atctactatt tttattagtt
     4621 tgttagtaat tgcaatgctg ttgagtagca ttttgtattt ttttgctgcc ttttggaacc
     4681 aacaaatttt tggaacaaat tatgaatatg aaatagttat tagggcaatg gcattagctc
     4741 tgccatggtt tgctattgct gtttttttac ttgccgtaat taatggtttg ggaaaattta
     4801 aagaagttat ttggattaat attgttggaa atataatagg attactggtt tcaattggaa
     4861 tgattttgca atataaaact ttgggagcct tactttctat tgtgattaca ccagctttat
     4921 tgttttttgt gacttattat tttatttcca aagagtttca tttctcaaaa ttcattcaat
     4981 ataaatctta tgatttacat gtaattaaga atctatcttc ttattcgctt atggcattgg
     5041 tttcttccgt tttggggcct ttggtttttt tggccattcg gaaaaatgta attgtagttc
     5101 ttgggataca agaagctggt ttttgggaaa caatgtcccg aatttcaacc tattatatga
     5161 tgtttgtttc cactatattg acggtttatt ttttgccaaa attggctgtt gccaaagata
     5221 atatagaaac aaaaaaagtc ttttggagct tttacaaaaa cattctacca ctttttgcaa
     5281 ttggattagt actgatttat ttttcaagaa cactacttat aaaattgctt tttgcaagcg
     5341 aatttctccc cgttacagac ttgtttttat ggcaattatt tggtgatgtt ttgaaggtag
     5401 cagcaatgat tttaggctat cagttttatg caaaaaaaat gaccttggct tttattattt
     5461 cggaaatggc gtcgctagct gttttgtatt tttctagcat ttatttgatt gctatttgtg
     5521 gaattcaagg aattgttatg gcccaagcct tagataattt tatttatttc ttggtattgg
     5581 ctatctattt tcgtaaaagt ttattttgaa tttttagttc ctatttttcg aatagattaa
     5641 ttaatgggaa tcttatttga attcgcacag aaaataggta tttttgaaaa aaaataaaaa
     5701 tgattccact atattttgat ccaggtactg gagctttgat tgtacaattt ttggcagcag
     5761 cagttgctgg tattttgttg ttttacagga ctgtattgca taagataaaa tctattttcg
     5821 gtattaaaaa caaagaagaa gatgatttta tggatgaaac tgataactcg gaagacacta
     5881 aataaatgga atcgaaacga aatacttcgt cttttagaga tccgtcgggt tatgttttta
     5941 tcgaggatga cagcgttaaa agagttgtaa atcacatcta ttttgagcag tacaatcaat
     6001 taacttctag tggtttttat caacttcttt tcgataaaaa atatttagtt tctcatcaag
     6061 aagtttctag aacagactca gaaattatta tcgaagcatc caagattcct tttataaatt
     6121 atccttatga atggagtttt ttgcaataca aacacgccgc tttactgact ttgaagattc
     6181 agaaattgtg tttagaaaat aattttacgc tgaaagatgc ttcagctttt aatattactt
     6241 ttcacgaagg gaaacccatt tttatagata ctttatcttt cgatttttat caggaaaaca
     6301 atccttggtt ggcttataag caatttgtaa tgcatttttt aggccctttg gttttgacaa
     6361 aatattttgg tcaagatcat ctcaaaactt tggctttgca tcttgaaggc atttcgttgc
     6421 aaaaactatc caatttattg cctttaaaaa gttattggag tccaatattg ttgactaata
     6481 ttcatttgtt ggccaaatac gataagaaat atgaatcgga taaaaagaag gtaaacaaca
     6541 atttatcaaa agcttctcaa atcaaattat tggacggatt gtatgattat attctgaatt
     6601 tatccgtaaa tgaaaagaca gaatgggatc attattacaa tcaaatcaat tataatgagg
     6661 ttgcttatca agtcaagaaa gagtttgtca aagattggtt tagctcaata aaggggaaat
     6721 ccttaattga tattggaggc aatgatggca ctttttcgag agaattaaag gaacttgctg
     6781 attttatgat tgttgctgat gttgatgcca atgccgtaga gcagaattat aaacaaattt
     6841 taaaaaacaa agaaaaatcc attcttccca ttgttgttga tgttttaaat ccagtggcaa
     6901 attacggttt taataatcaa gaacgttttt cctttattga tcgagtagaa gaggcaaatt
     6961 tggatgctgg tttggctttg gcggtgattc atcatattac attgtctgga aatattccct
     7021 tctctttatc agcacagttt ttctccaaaa tggcacccaa tttattaatt gaattcccaa
     7081 caagagctga ttcttgggta cagtttttat tggatagcaa aagagaattc aaaagccatt
     7141 ttgattttta taatgaagag aattttgaaa atgaatatgc tgtttatttt gaaattgttc
     7201 acaaagtaaa aattgattct tcagaaagaa ttttatacag tttaaaacga cgataaatga
     7261 atttgaaaat aaaagacaaa ttaaattatt ttttgaaaaa tgatgaggat tatcccattt
     7321 tatcggggtt tagtgtcggg ttttatgcga tggtttttta tttttccaac aatttcgatt
     7381 tagtcaattc ttgggagcag accttatttt ttttatttta ctatataatt gtatctgttg
     7441 gagttgtttt cgttatttcg aaaataatag aaaaaagtaa gtataatagc tttgtttcgc
     7501 aagctatttt tgtttcgatg ctattgctat taccgattta tttgtttgga atttcaaaca
     7561 ttttggattc caataaaaaa atgggtttag tattgattct aattttgtcc ttgttcattt
     7621 ttaaaataaa aaattacaaa tattgtattg ttctcttgtt tttgatgtcg gttattccgg
     7681 ttattaaatt gggaaaaata atttacttaa attataccaa ttcaacagaa tggaagcttc
     7741 aacccgatgc tattttaaaa gccgaattca aaaaaaaacc aaatatttat tacattcaac
     7801 cagatggtta tgctggagaa gtaaatttga aagggaattt atatcgttat gacaattccg
     7861 cattcgatac ttttttgaaa gagaaaaaat ttactttata tccaaattat cgcagtaatt
     7921 acgaatcgac tttgttttct aattcgtctt gtttttttat gaaacatcat ttttcgaatg
     7981 aattttcgaa atttaagtat gccagagatt tgattattgg cgaaaatcca gtgcttaaaa
     8041 tcttaaagaa taataattac aaaacatttt ttataaccga aaggccttat ttattgatga
     8101 atagacctga agttttttat gattattgta attttaaagt gggtgaactt ccttatttaa
     8161 gtgatggttg gtctgatttt agagaaatta caaacactat taaaacacaa atcataaaca
     8221 ataaagatac aaataatttt ttctttatcg aaaaatttaa gccgggtcat attgcaactt
     8281 ataaattcgc tggaactaac aaagaaaaag aaagaataaa atatattgaa agtattgaag
     8341 aggcaaacca atggttgcag caaataattg tttttatcga agcaaaagat ccaggagcta
     8401 tgataattat tggtgctgac cacggtggat ttgttggttt tgattatgct ttagaagctc
     8461 aaaaaaaagt gactgactcc atgttaattc aatcaatttt cggagccaaa ttagctataa
     8521 agtggaataa tgtgcaacat tcagaatatg attctaaatt aaaaactcct gtgaatttat
     8581 ttagaaccgt tttctctttt ttgagtgagg ataaaaactt attgaatcat ttgcagcctg
     8641 atgcaagtta caattgctat gatcctacag attttacaaa agtgtataag gctattgatg
     8701 aaaacggaag tacagctaaa ttttaaaaag caagacccaa taatgagctt aacgagtttg
     8761 tttagagggt taagtatcaa tactagtaat tatgttgtca aagattttaa atagcacttt
     8821 attagccaag attaatagta aggaaatcaa aatacttgtt gtttttggat tattatatcg
     8881 cattattttt tctgtttttt actttcatat aacaatttat cctgactctg aagggtatct
     8941 ttttctgagt caaaagattt taaattttaa tttaagccac tacaatggcg aaagaagttt
     9001 aggttatcca ttgttgattt cttttgcttt cggttcaacc ataataaccc ttttatatca
     9061 attcatttta gggattatta cttcaattta ctggtacaaa acgctaatca atttgaggtt
     9121 ttcttcaaaa aaagcacttt gtgttactct ttttcttcaa agctttatta atgtttactt
     9181 ttatgaaact gctattcttg tagaatcttt aagcttgttt tttatttcag taattgtctt
     9241 tatgctaact gataattatt ttgaaaacag aagtttgaaa aatgattttt taataggttt
     9301 cataattgct tatttagttg ctataaagcc attttatatc tatattccct ttgtaattta
     9361 tttgtttgtt attttaaaaa accacaattt aaaaaggcta atatttaata aaattgttat
     9421 tttgattttg ccattaatta tgtttttcgg ctggtcctat gttaataaaa taaatactgg
     9481 acattttgtt tcaacaactt tttttggact taatctttct caaaattgtg tttattttgc
     9541 tgaaaaagct ccaaaagagt tcgattggat tgctaaacca tacgtaaaat atagggaaat
     9601 ggcaattaag caagataaag atgtggctat gtcaatttgg tttgcaagaa aacatggtgc
     9661 ttttgcttct tataacttga aattgtgtga tttgtcaagt gaattggggg actttgcaaa
     9721 agaaaccatt aggaacaacc caattgatta tttaaaacaa gtttttactc gttcatggtt
     9781 tgatttttgg aaaccttcaa tttattggaa tttcgacaaa tttaattttg aatatgcaaa
     9841 gaaaattgtt ctgctcattt ggtatattca agttccaatt actgtttttt ttaaattcac
     9901 ttttgtattg cttattccaa ttttatccta ttttgcattt aaaagaagag agatttcaat
     9961 tgaatttata gtgtcaataa ttgttttttc tggttctatt ttgcagggtc tcgctactta
    10021 tggaacaaat aatagattta gtttcccatt tgaatttatt atgatacttg ttgtaatatc
    10081 attttttaag agggaaattt atttttccaa acatttaaat acatttcggc aatttttaaa
    10141 taattatgct ctttttcgat aaaggttctt gcacgatttc caatggcaat tatttcttcg
    10201 ggattttcaa ttaaaaaaga caattctgtt actaaataat ctac
//
LOCUS       DAOSNN010000070        10224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6550_length_10224_cov_5.698790, whole genome shotgun sequence.
ACCESSION   DAOSNN010000070 DAOSNN010000000
VERSION     DAOSNN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10224
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6550_length_10224_cov_5.698790"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /gene="dgt"
                     /locus_tag="PLQ70_05150"
     CDS             complement(<1..603)
                     /gene="dgt"
                     /locus_tag="PLQ70_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HQE34036.1"
                     /translation="MNWEQLLSLKRQGDKGKRLRIEQDDTRLGFEVDYDRIIFSSAFR
                     SLQDKTQVIPLSKTDFVHTRLTHSMEVSVVGRSIGRLVGKKIIEKYPHLKEVHGFQAN
                     DFGAIVAAASLAHDIGNPPFGHSGEKAIGEYFSIGKGQQYKDQLSAKEWQDLIDFEGN
                     ANGFSVLTASRPGIEGGLRISYATLGAFMKYPKESLPKKPT"
     gene            complement(760..1509)
                     /locus_tag="PLQ70_05155"
     CDS             complement(760..1509)
                     /locus_tag="PLQ70_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE34037.1"
                     /translation="MKKLQNKVAVITGGNSGIGLATAKLFAEQGAKVAITGRNKKTID
                     EAVFEIGNASIGLVSDVSDLNSIDTTYGTIKNTFGKIDVLVVNAGVFIAAPLADFTEE
                     MFDQTSDINFKGVFFTIQKALPLLNDGASIVITASTVAHKGFATTAAYSASKAAVRSL
                     ARTLSAELLDRNIRVNVLSPGPIDTPLFERGGASKEEANGTKTHFASGIPAKRLGTSE
                     EMAAGFLYLASDDSKFMIGGELLLDGGAATL"
     gene            complement(1573..1860)
                     /locus_tag="PLQ70_05160"
     CDS             complement(1573..1860)
                     /locus_tag="PLQ70_05160"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQE34038.1"
                     /translation="MITSLSIKQVEKISKALGDPYRLKIMKIVSNSESCVQCCDVSAE
                     FNLAQSTMSHHIKQLIDADLLIADKDGRNLKFIVNKEVCTAYADYINSLVL"
     gene            1984..3732
                     /locus_tag="PLQ70_05165"
     CDS             1984..3732
                     /locus_tag="PLQ70_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE34039.1"
                     /translation="MKILYAYIKTHKTLLYIALFLATINQCFSLFDSIIIGKLLNECG
                     VGVANFNHNQLSFTKAVLGWLALSLGAAMVSRIAKNFQDYYTNIIIQRTGAQMYTDGI
                     QKALQLPFQDFEDQRSGETLGKLQKVKTDCEKFITLSISLIFQSIVGIVFVVVYAVSI
                     HWLLGPIFLATVPVIAFISSFLGKRIKKVSKEILGETTALAGATTESLRNIELVKSLG
                     LTEQEVNRLNTTTLKILGLELKKVRFIRSLSFIQGTTVHFMRTTLVFALYMFIFNGII
                     KPGDMITLMFFSFFLFNPLQELGNVIATFNETKASMDNFAHLMDSESEITPENPKTIG
                     AINHLRFENISFKHQTASSYAVKNISFDAKAGETIAFVGPSGSGKTTLVKMLVGLYNP
                     AEGAIFYNEKNAKEIDLTELRQQLGFVTQDAQLFSGTIKDNLLFVKPNATDEEIDDVL
                     QKSACQNLLARAENGIYTTIGEGGIKVSGGEKQRLSIARALLRNPHLLLFDEATSALD
                     SITEEEITKTIRSISSKQDQITVLIAHRLSTIMHADKIFVLEQGQIIEQGKHQDLLDE
                     KGLYYAMWRQQIGERK"
     gene            complement(3767..4225)
                     /locus_tag="PLQ70_05170"
     CDS             complement(3767..4225)
                     /locus_tag="PLQ70_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE34040.1"
                     /translation="MNTIKILRTTSENQDFAKLVIALDAYLKILDGEDHEFYAHFNKT
                     NLLKNAVICYENDIPVGIGAYKEYEPKTAEIKRMYTLPEYRGKGIASKIIHELELWAK
                     EENYTTSILETGFKQEDAIYLYQKLGYTIIENYGQYKGVENSVCMKKSLD"
     gene            complement(4300..4785)
                     /locus_tag="PLQ70_05175"
     CDS             complement(4300..4785)
                     /locus_tag="PLQ70_05175"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQE34041.1"
                     /translation="METKFTIPHYILASKTTRLLNFVIDIFFINILSVFVYLLSSFVK
                     VNANFPKLSNWIDSLDQYEKIIYRIIICFFYYGLTEYLLSKTFAKYFTKTIVVSEDGS
                     KPNFITILARTSLRIMPFECLTFLRGREPGFHDDYSKTFVVIKDKLENSKKEFLEFQN
                     F"
     gene            complement(4848..7250)
                     /locus_tag="PLQ70_05180"
     CDS             complement(4848..7250)
                     /locus_tag="PLQ70_05180"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HQE34042.1"
                     /translation="MYVVKRDGHKEPVMFDKITDRIKKLCYGLNDLVDAVKVAMRVIE
                     GLYDGVSTSELDNLAAETAASMTIAHPDYAQLAARIAISNLHSNTKKSFSETMNDMYH
                     YINPRTNMESPLLSDQVHKVIMENAEFLDSHIIYNRDFNYDYFGFKTLERSYLLKING
                     KIVERPQHMLMRVSVGIHLDDLKSVIETYDLMSKKYFTHATPTLFNAGTPKPQMSSCF
                     LLAMKDDSIDGIYDTLKNTAKISQSAGGVGLSIHNVRATGSYIRGTNGTSNGIVPMLR
                     VFNDTARYVDQGGGKRKGSFAVYIEPWHADIFEFLDLKKNTGKEEMRARDLFFAMWTS
                     DLFMKRVQENGDWTLMCPNECPGLYDVHGEEFEKMYTDYEKAGKGRKTIKAHELWEKI
                     LESQIETGTPYMLYKDAVNRKSNQKNLGTIRSSNLCTEIMEYTSADEIAVCNLASLSL
                     PMFVENKKFNHELLFTVTKRVTRNLNKVIDRNYYPVQEAENSNMRHRPIGLGVQGLAD
                     AFIMLRMPFTSDEAKKLNQEIFETLYFAAVTASMEMAKEDGPYSTFKGSPISQGEFQH
                     NLWGLKDEELSGRWDWASLRKEVMEHGVRNSLLVAPMPTASTSQILGNNEAFEPYTSN
                     IYTRRVLSGEFIVVNKHLLNDLVERGLWNETLKQEIMRNNGSVQNLDIPQDLKELYKT
                     VWEMSMKDIIDMSRQRGYFVDQSQSLNLFMQNANYSKLTSMHFYAWQSGLKTGMYYLR
                     TKAAVDAIKFTLNNDKKAEPAPAVEVEATTSSVEMSVEEYRAMIELAKNAGPEDCEMC
                     GS"
     gene            complement(7475..8452)
                     /locus_tag="PLQ70_05185"
     CDS             complement(7475..8452)
                     /locus_tag="PLQ70_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HQE34043.1"
                     /translation="MSQIEPILQENKNRFVIFPIKHQDIWEFYKSMEASFWTAEEIDL
                     AQDLNDWNNKLSEDEKYFVKHILAFFAASDGIVNENLAQNFVNEVQYAEAKFFYGFQI
                     MMENIHSETYSLLIDTYVKDEAEKDELFNAIERFPAIQKKAEWALKWIESDSFAERLI
                     AFAAVEGIFFSGSFCSIFWLKKRGLMPGLTFSNELISRDEGVHCDFAVHLHNHHLKHK
                     VSKARIREIIVDALNIEREFITESIPVSLIGMNATLMTQYLEFVTDRLLVELGCKREY
                     NTANPFDFMDMISLQGKTNFFEKKVAEYQKSGVMNTDSDAQKISFDADF"
     gene            complement(8698..9270)
                     /locus_tag="PLQ70_05190"
     CDS             complement(8698..9270)
                     /locus_tag="PLQ70_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HQE34044.1"
                     /translation="MFQLGKTIVSEDILEKEFVCNLSACKGACCVDGDAGAPLSEAET
                     KIMEAIYPKVKPFLRKEGIAAIEAQGTWTKGTEGDLETPLIDNKDCAYVIFDGKTALC
                     GIEQAYNQGIVDWKKPVSCHLYPIRVKDFTEFAAVNYDKWDICDDACFLGKELEVPVY
                     KFVKEALIRRFGEDWYFELEKVAKELKEER"
     gene            9474..10049
                     /locus_tag="PLQ70_05195"
     CDS             9474..10049
                     /locus_tag="PLQ70_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HQE34045.1"
                     /translation="MELDFKEIATVGMVLFAVIDIVGTIPVMVDLKSKHGHIESEKAS
                     IVAGMIMIVFLFVGEELLNLIGIDVHSFAVAGSFVLFFLALEMILGIRIYRDEESSSA
                     SIVPIAFPLIAGAGTMTTLLSLKAQYQTVNILIAIVLNIIVVYIVLKSSAKIERMLGK
                     NGLGVIRKAFGVVLLAIAVKLFAGNVKDLFL"
BASE COUNT         3095 a         1830 c         1757 g         3542 t
ORIGIN      
        1 ggttggtttt ttgggtaagc tttcttttgg atatttcata aatgcaccca aagttgcata
       61 ggaaattcgc aatccgcctt caattccggg acgacttgct gtgaggactg aaaaaccgtt
      121 ggcattgcct tcaaaatcga ttaaatcctg ccattctttt gccgaaagtt ggtctttgta
      181 ttgttgtcct tttccaatag aaaaatattc tccaatggct ttttcaccag aatgtccaaa
      241 aggtggattt ccgatgtcgt gtgccaatga agcggcagcc acaatagctc caaaatcatt
      301 ggcctgaaaa ccgtgaactt cttttagatg cggatatttt tcgatgattt tctttccaac
      361 caatcttcca atggaacgtc ccacaaccga aacttccata ctatgagtca gtcgggtatg
      421 gacaaaatcc gtttttgaaa gtggaataac ctgtgttttg tcctgcaaac ttctgaaagc
      481 cgacgagaat ataattcgat cataatcgac ttcgaaaccc aaacgggtgt cgtcttgttc
      541 aattcgtaat cgttttcctt tgtcgccttg gcgttttaaa gacaatagtt gttcccagtt
      601 cattgtgatt ggagattttg gatttttgaa ttcaaagata atagaaaata gaatcaaatc
      661 gccaatgctg tgatttgctt tattctctta tcaaaaaagc cctagtcgat ttattcaaca
      721 agagcttctt cttttaaaaa caaattaata actaaaattt tatagtgttg ctgctccacc
      781 atccaataac agctcaccac caatcataaa cttagaatca tcggaagcca gataaagaaa
      841 tcctgccgcc atttcttctg aagttcctaa gcgtttggct ggtattcctg aagcgaaatg
      901 tgtttttgtt ccgtttgctt cttctttgga agctccacct ctttcaaata aaggcgtatc
      961 aataggccca ggagaaagta catttacacg tatatttcta tctaataatt cggcagataa
     1021 agttctggcc agtgaacgaa ccgctgcttt ggaagcagaa taagccgcag tagttgcaaa
     1081 acctttgtgc gctacagtcg aggcagtaat aacaatcgaa gcgccatcat ttagaagagg
     1141 taacgctttt tgaattgtga agaaaacacc cttgaagtta atatcgctcg tttgatcgaa
     1201 catttcttcc gtaaaatctg ctaaaggtgc tgcaataaat acacctgcat tcacgactaa
     1261 tacatctatt tttccaaaag tatttttaat agttccgtaa gtagtatcaa tgctgttcaa
     1321 atccgaaaca tcgcttacca aaccaataga agcatttcca atttcgaaaa ccgcttcgtc
     1381 aatggtcttt ttattgcgac cagtaatggc cactttagct ccttgttctg caaataattt
     1441 tgctgttgct aatcctattc cgctgttacc acctgtaatt acggctactt tgttttgtag
     1501 ttttttcatt tttatttatt gttttaagta tttaaatatc taaatatttc gatgttttta
     1561 attaaaaaaa agctaaagca ctaaactgtt gatataatct gcataggcag tacaaacttc
     1621 tttattaacg ataaatttca agttacgtcc gtctttgtca gctatcaata aatcagcatc
     1681 aatcaattgt tttatatgat gcgacatcgt tgattgagct aaattaaatt cagcagaaac
     1741 atcacaacat tgcacacaac tttcgctgtt gctgacaatc ttcataattt tcaaacgata
     1801 tggatcgccc aaagcttttg agattttttc gacttgtttt atgcttaaac ttgtaatcat
     1861 agagcaaaga taaaaatata tttttaatat atcgaaatat ttcgatatat tttttaaatt
     1921 tgcactataa ataagttcaa cagttacttt tagcttgaat tatttccttt taaaacattt
     1981 tctatgaaaa tattatatgc ctacatcaaa acccataaaa cgcttttata cattgcgcta
     2041 tttttagcca ccatcaatca gtgtttttct ttatttgatt ccattattat tggtaagctg
     2101 cttaatgaat gtggcgttgg tgttgccaac ttcaatcaca atcagctcag ttttaccaaa
     2161 gcggttctag gctggctggc gctatcttta ggcgcagcaa tggtttcgag aattgccaaa
     2221 aactttcagg attattatac caatatcatc attcaacgaa cgggtgcgca aatgtacaca
     2281 gacgggattc aaaaagcctt gcaattgcct tttcaggatt ttgaagacca gcgaagcgga
     2341 gaaactttag gcaaacttca gaaagtaaaa acagattgcg aaaaattcat caccctatct
     2401 atttcattga tttttcagtc gatagttggg attgtctttg tggtggttta tgctgtaagt
     2461 attcattggc ttttggggcc aatattcttg gcgactgttc cggtgattgc tttcattagc
     2521 tcgtttttag gcaaaagaat caaaaaagtt tctaaagaaa ttttaggaga aaccacggca
     2581 ttggctggag ctacaaccga atcattgcga aatattgaac tcgtaaaaag tttaggctta
     2641 accgaacaag aagtgaatcg attgaacaca acaacgctaa aaattcttgg cttagaactc
     2701 aaaaaagtac gtttcattcg gtctttgagt tttattcaag gaacaacggt tcattttatg
     2761 cgaactactt tggtttttgc gctttatatg ttcattttta atggcatcat caaaccagga
     2821 gatatgatta cattgatgtt tttctctttc tttttgttta atccgttgca ggaattgggg
     2881 aacgtgattg ctactttcaa tgagacaaaa gcatctatgg ataattttgc ccacttaatg
     2941 gattccgaaa gcgaaattac acctgagaat cctaaaacta ttggcgctat caatcacttg
     3001 cgttttgaga atatttcgtt caaacaccaa acggcaagtt cttatgctgt aaaaaacatt
     3061 tcttttgatg caaaagctgg cgaaaccatt gcgttcgtgg gcccatcggg aagtggaaaa
     3121 actactttag taaaaatgtt ggtcggatta tacaatcctg ccgaaggcgc tattttttac
     3181 aatgaaaaaa atgccaaaga aattgacctc actgaattaa gacaacagtt gggttttgta
     3241 acccaagatg cccaactatt ttcgggaacg ataaaagata atttattatt tgtgaaacct
     3301 aacgctaccg atgaagaaat tgatgatgtt ttacaaaaat cggcttgtca aaatttattg
     3361 gctcgtgctg aaaatggcat ttacaccacc attggcgaag gcggaataaa agtttcagga
     3421 ggcgaaaaac agcgtttgtc aatcgcaaga gctttactta gaaatccgca tttactgctt
     3481 tttgatgaag ccacttctgc attagattca attaccgaag aagaaatcac caaaactatt
     3541 cgaagtattt cgtccaaaca agatcaaatt acggttttga ttgcgcaccg tttatcaacc
     3601 attatgcacg ctgataagat atttgttctg gaacaaggac aaatcatcga acaaggcaag
     3661 caccaagatt tattggatga aaaaggcttg tattatgcaa tgtggagaca acaaatagga
     3721 gagaggaagt agttttatgc gattgtattt gttctaaaaa cacgttttaa tctaatgatt
     3781 ttttcataca aacactattt tcaacacctt tatattgacc atagttttca atgattgtat
     3841 atcctaattt ttggtataaa tagattgcat cttcttgttt gaagcctgtt tcgaggatgc
     3901 tagtagtgta attttcttct tttgcccaaa gttctaattc gtgaataatt tttgaagcaa
     3961 ttccttttcc acgatattcg ggtaaagtgt acattcgttt tatttctgct gttttaggct
     4021 catattcttt gtaagcccca atgcctacag ggatgtcatt ttcatagcaa atcaccgcgt
     4081 ttttcaataa attagtttta ttgaaatggg cataaaactc gtgatcttct ccgtctaata
     4141 tcttcaaata agcatcaagt gcaataacca attttgcaaa atcttgattt tctgaagtgg
     4201 ttcgaagaat tttaattgta ttcattttgt tttaaataat ttatgggtac aagcttgacg
     4261 gttgcgcttg cggagaggaa gtagtgtcaa cttattatct taaaaatttt gaaactctaa
     4321 aaactccttt ttactatttt ctagtttgtc ttttattacc acaaatgttt tagaatagtc
     4381 atcgtgaaaa cctggctctc tacctctcaa aaaagtaaga cattcaaacg gcataattct
     4441 taaagaagtt ctggctaaaa tagtaatgaa atttggtttt gaaccatctt ctgaaacaac
     4501 aattgttttt gtaaaatatt tagcaaaagt ttttgaaagc aaatattcgg ttaatccata
     4561 atagaaaaaa caaattatga ttctgtaaat aattttctca tattgatcta atgaatcaat
     4621 ccaatttgac aatttaggaa aatttgcatt gacctttacg aaactggaca ataaatacac
     4681 aaatacagac aatatattga tgaaaaatat atcaataaca aagttcaata aacgagtggt
     4741 ttttgatgct aaaatgtagt gtggtatcgt gaattttgtt tccatttaat taaaaaaaaa
     4801 cagttaccaa aaagataact gtttcaaata taagataatt tttcatttta agacccacac
     4861 atttcgcaat cttctggacc agcgtttttg gccagttcga tcattgctct gtattcttcg
     4921 acgctcattt cgactgaaga tgtagtggct tcaacttcta cggctggtgc tggctctgct
     4981 tttttatcgt tgttcaacgt aaatttaatc gcgtcaactg ctgctttggt tctcaaataa
     5041 tacattccgg ttttcaatcc agactgccaa gcgtagaaat gcattgaagt caatttagag
     5101 taattggcgt tttgcataaa caagttcaac gattgcgatt gatctacaaa ataaccacgt
     5161 tggcgagaca tatcgataat gtctttcatc gacatttccc aaacggtttt gtacaattct
     5221 ttcaagtctt gaggaatatc gagattttga acagaaccat tgttacgcat tatttcttgt
     5281 ttcaatgttt cgttccaaag accgcgttct accaaatcat tcagtaaatg tttgttaacc
     5341 acaataaatt ctcccgaaag cacacgtcta gtataaatat tagaagtgta aggttcgaat
     5401 gcttcgttgt ttcctaaaat ttgtgaagtc gaagcggttg gcattggtgc taccaacaat
     5461 gagttacgaa ctccgtgctc cataacttct tttcttaaag atgcccagtc ccagcgtcct
     5521 gaaagttctt catctttcaa tccccaaagg ttgtgttgga attctccttg cgaaattggc
     5581 gatcctttaa aagtagaata tggaccatct tcttttgcca tttccatcga agcggttacg
     5641 gctgcaaagt ataaagtttc gaaaatttct tggtttaatt ttttagcttc gtcgctagta
     5701 aaaggcatac gcaacataat aaaagcatcg gcaagacctt gtactcctag accaattggg
     5761 cgatgacgca tattggagtt ctcggcttct tgaacaggat agtaatttct gtcgattact
     5821 ttattcaagt ttctagtcac tcgtttagtt actgtgaaca ataattcgtg gttgaatttt
     5881 ttgttttcta caaacattgg taacgaaagt gaagccaaat tacaaaccgc aatttcatct
     5941 gcagaagtat attccataat ctcggtacac aaattcgatg aacgaatggt tcctagattt
     6001 ttttggtttg attttctgtt aacagcatct ttgtacaaca tatatggggt tccagtttcg
     6061 atttgcgatt ctagaatttt ctcccacaat tcgtgtgctt tgatggtttt tctaccttta
     6121 cctgcttttt cataatcggt gtacattttt tcgaattcct caccgtgaac gtcatacaaa
     6181 cctgggcatt cattcggaca cattaatgtc caatcgccat tttcttgcac acgtttcata
     6241 aataaatccg aagtccacat cgcaaagaat aaatctctgg cgcgcatttc ttcttttcca
     6301 gtattctttt tcaagtctaa gaattcgaaa atatcagcat gccaaggttc aatgtaaaca
     6361 gcaaaacttc ctttgcgttt tccaccacct tgatctacat agcgagccgt atcgttaaat
     6421 actctcaaca ttggcacaat tccgttggat gttccgtttg tgccacgaat atatgaacct
     6481 gttgcacgaa cgttgtgaat agaaagtcct actccaccag ccgattgcga aatttttgcg
     6541 gtgtttttta aggtgtcata aattccatca atactatcgt ctttcattgc caaaaggaaa
     6601 caagaagaca tttgaggttt tggagttcca gcgttgaata aagttggagt agcgtgagta
     6661 aagtattttt tcgacatcaa gtcgtaggtt tctattaccg attttaaatc atctaagtga
     6721 attccaaccg aaacacgcat caacatatgt tgcggacgct ctacgatttt tccgtttatt
     6781 ttcaataaat aagaacgctc caaagttttg aaaccaaaat aatcgtagtt gaaatctcta
     6841 ttgtatataa tgtgtgaatc caaaaattca gcattttcca taatcacttt atgtacttgg
     6901 tctgaaagca aaggagactc catattggtt ctcggattga tgtaatgata catatcgttc
     6961 atggtttccg agaatgattt tttagtatta gaatgcaaat tcgaaatggc aattctagct
     7021 gctaattgtg cataatcagg atgcgcaatg gtcatagaag cggcagtttc tgctgcaaga
     7081 ttatctaatt ctgatgttga aactccatcg taaagtcctt caattactct cattgccact
     7141 ttcacggcat ctactaaatc attcaaacca tagcatagtt ttttaattct atctgtgatc
     7201 ttgtcgaaca ttacgggctc tttgtggccg tctcttttta ctacgtacat aatcttgttg
     7261 ttgtttttta aaacagaaaa tcccttttct attttggtga atttgtatta tttttttggg
     7321 aaactaatcc cgagggttta ttgccgcaaa ggcgctaggg cgcaaagttt tttagtttta
     7381 tgttataaaa atctttgtca aaatttaaaa ctttgacaaa gagtactaac aattgttttt
     7441 tttagcgact ttgcgtcttt gcggtaaaaa aatattaaaa atcagcgtcg aagctaattt
     7501 tttgagcatc agaatcggta ttcattactc ctgatttttg atattcggct actttctttt
     7561 cgaagaaatt agtttttcct tgaagcgaaa tcatatccat aaaatcaaaa ggattggctg
     7621 tgttgtattc tcttttgcaa cctaattcta ctaataatct gtcggttaca aattctaaat
     7681 attgagtcat caaagtggca ttcattccaa ttaaactcac cggaatagat tcagtaatga
     7741 attctctttc gatatttaaa gcatcgacaa taatttctct gatacgtgct tttgatactt
     7801 tatgtttcaa atggtgattg tgtaaatgca cagcaaaatc acagtgaacg ccttcgtcac
     7861 gcgatattaa ttcatttgaa aaggttagtc ctggcatcaa acctcttttt ttcaaccaga
     7921 aaatagaaca aaaacttcct gaaaagaaaa ttccttctac cgcagcaaaa gcaattaatc
     7981 tttcggcaaa tgaatcggat tcgatccatt tcaaagccca ttctgctttt ttctgaatag
     8041 ctggaaatct ttcgatggca ttgaataatt cgtccttttc agcttcatct tttacataag
     8101 tgtcaatcaa aagcgaatag gtttcgctat gaatattttc catcattatc tgaaagccat
     8161 agaagaattt tgcttcggcg tattgcactt cgttaacgaa attttgagct aaattctcgt
     8221 ttacgattcc gtcagaagct gcaaagaagg caagaatatg ttttacgaaa tatttttcgt
     8281 cttcacttaa tttattgttc caatcgttta aatcttgagc taaatcaatt tcttctgcag
     8341 tccagaaact agcttccata cttttataaa attcccaaat atcttggtgt ttaataggaa
     8401 aaattacgaa tcgatttttg ttttcttgta agattggttc aatttgtgac attgcgattt
     8461 ttattttttt gagattttaa cgaaatatgt gcttttcgtc gattacaaag attgtgaatt
     8521 atcacggtta ttaaaagcca aacttattca caatcggctt gagtttttaa caatttgaat
     8581 atttttcata caaatcacaa ataattgtat gtcaaatagt tataaacagc aaaatataga
     8641 aaacacccta aaacataggg atttaaaaga gataaaaaag aaagtttcta aattatttta
     8701 acgttcttct ttcagttcct tggcgacttt ttcgagttca aaataccagt cttcgccaaa
     8761 tcgtctaatg agtgcttctt tgacaaattt atatacagga acttccaatt ctttacctaa
     8821 aaagcaggcg tcatcgcata tgtcccattt gtcataattg actgcggcaa attctgtaaa
     8881 atcttttaca cgaatagggt ataagtggca agaaacaggt tttttccagt ccacgattcc
     8941 ttgattgtag gcttgttcga ttccgcataa tgcagttttt ccgtcaaaga tgacgtatgc
     9001 gcaatcttta ttgtcaatta aaggtgtttc tagatcgccc tcggtgcctt tggtccaagt
     9061 tccttgtgct tcgatggctg caataccttc tttgcgtaga aatggtttta cctttggata
     9121 aatggcttcc ataattttgg tttcggcttc gcttaatggt gcgccagcgt ctccatcgac
     9181 gcaacatgcc cctttgcaag ctgataaatt acaaacaaat tctttttcga gaatatcttc
     9241 cgatacgatg gtttttccta gttgaaacat tttgcaaata taggtttcaa ttctgaattt
     9301 tgactgttaa atttttagtt ttttaattca aaaattattc cattatcata aaataattta
     9361 gtttaaaact gatgttgttg gtcaatgcac ttgatggttt tgctaaaaat taacccttat
     9421 ttatgagtta atcgcataga ataccttact tttgcctcgt aaaattatga aatatggaat
     9481 tggattttaa agaaattgcg actgtaggaa tggtgctttt tgctgtgatt gatattgtag
     9541 gaacgattcc tgttatggtc gatttgaagt ccaaacatgg gcatattgaa tcagagaaag
     9601 cctcaattgt agcaggaatg attatgattg tttttctatt tgttggtgaa gaattgttaa
     9661 atctgattgg tattgatgtg cattcatttg ccgttgcagg ttcttttgta ttgttctttt
     9721 tggctcttga aatgatttta ggaattcgca tttatcgcga cgaagaatcc agttcggcat
     9781 cgattgttcc tattgctttt ccgttgattg caggagctgg aacaatgacg actttattgt
     9841 cattaaaagc acaatatcaa actgtaaata ttcttattgc catagttttg aatattattg
     9901 tggtttatat cgttttgaaa tcatcagcca aaatcgaaag aatgttagga aaaaatggtt
     9961 tgggagtaat aagaaaggct tttggggtcg ttcttttggc aatagctgtt aaattatttg
    10021 ccggtaatgt taaagatttg ttcctgtaaa ttaaattttt atacatttac ccagcaattt
    10081 acaaaaactt acaaaatgaa actttttaca aatatattgg tcgttttagc tattgggcta
    10141 atccttttca atattagcct gattgacttt aaaaatcctt ttgaaggcaa cagtatgatt
    10201 gctgttattg gaattgcggc ttca
//
LOCUS       DAOSNN010000071        10111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6655_length_10111_cov_5.470167, whole genome shotgun sequence.
ACCESSION   DAOSNN010000071 DAOSNN010000000
VERSION     DAOSNN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10111
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6655_length_10111_cov_5.470167"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLQ70_05200"
     CDS             complement(<1..416)
                     /locus_tag="PLQ70_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34046.1"
                     /translation="MSEAAVKTGHPKGLWVLFGTEMWERFNFYGMRAILTLFMVNALM
                     LGESNASIIYGGFLALCYLTPLLGGFISDKYLGNRSCILLGGSLMAIGQFSLFYSATI
                     YDSNLDFSKAIFWLGLFVIIFGNGFFKPNISSMVGS"
     gene            complement(443..2617)
                     /locus_tag="PLQ70_05205"
                     /pseudo
     CDS             complement(443..2617)
                     /locus_tag="PLQ70_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
     gene            2741..4129
                     /locus_tag="PLQ70_05210"
     CDS             2741..4129
                     /locus_tag="PLQ70_05210"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HQE34047.1"
                     /translation="MKLLKPYLCTLSSHNQLRMNNAVAGFSKLSKEEKINWIAKEYFS
                     SPQEALGILKKYWNSDEKLQKLHDEFIENTISNFYIPLGVAPNFLINGKYNSIPMAIE
                     ESSVVAAASKAAKFWSTRGGFKARVLNTEKIGQVHFIYKGDESKLESFFAIIKAKFFS
                     NTQNITKNMQKRGGGILDIELKVKTHLLPNYYQLHATFDTKDSMGANFINSCLEQFAK
                     TLQEEAQLWDLFSEEEKNIEVVMSILSNYVPNCIVRAEVSCPVEDLVEKHIPNPKAFA
                     ERFIRAVQIAEVEPFRAVTHNKGIMNGIDAVVLATGNDFRAVEAGIHAYASRKGSYSS
                     LSHAKIENDIFTFWLEVPLALGTVGGLTSLHPLVKFSLEMLENPSAEELMQFVAVAGL
                     AQNFAALRSLTTTGIQDGHMKMHLNNILNQFEATEDERHLIHKHFKHQVVSHSSVVEY
                     IETLRNSEQFRL"
     gene            4185..5102
                     /locus_tag="PLQ70_05215"
     CDS             4185..5102
                     /locus_tag="PLQ70_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HQE34048.1"
                     /translation="MKKEFYSNGKLLITGEYLVLDGAKAFALPTKFGQKLIVEKGKHP
                     GIKWTSFDADGSKWLDLQVSFEDILNKNTLVESESVKNTLIEILHQGYLLNPNFIENS
                     DGFAITTQLTFPRNWGLGTSSTLINNIAQWIEVDAFVLLNNSFGGSGYDIACAQNDSP
                     ILYHLNNGKPIIEKVQFEPKYSENLYFVYLEKKQSSKASIVSYYNNKNEGLDQKIVEN
                     NKITSAVLKAKTLADFAIALEEHEAEMSTILEMQTIKESLFPDFDGVIKSLGAWGGDF
                     VLVLSDENPVEYFNEKGFKTIVSYKDMIL"
     gene            complement(5195..7294)
                     /locus_tag="PLQ70_05220"
     CDS             complement(5195..7294)
                     /locus_tag="PLQ70_05220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQE34049.1"
                     /translation="MAVLQKIRQRSALLIVVIGFCLFAFIAGDVFTKGNFGKSSNEVG
                     SVNGKDIPFEDFRVKVSNVEKSQQGVTPTAAANRVWDQEVSIALLTSEFDKLGLRVGA
                     DHIMQILESDQNIGKNPMFLNSAGRFDKDKFKEATKTNPEIAQYVKDREKDAELNAKY
                     QMYATMIKAGSYTTESEGKLKYQMEADKVNFAYVAGLYSTIKDSDVKVSDDDIVAFMK
                     KNEKKYKSDETREIEYVLIEDKASPEDEAEVKAKINGLLSGSVVYNTKTGKNDTLPGF
                     RSATNVVEFVNSNSDVPYDSTYVAKKDLPAIDADKLYNLAPGEIYGPYKFGKYYCLSK
                     SLGRKVGVNAKASHILISYEGTQVPNQKEKRTKEQAKAKAEEILAQVNANPNSFLMLA
                     FTASDDSSAQQGGDLGFFGPNQMVKPFNDFVFNNPIGKVGLVETPFGYHIIKITDKQD
                     GVRLATVAQKVEASEATSDKVFTQATQFEIDANAKDFAKAAAALKLTVSPAVTAKLMD
                     ESFGPLGNQRNIVRWAFEDGTDVGAIKRFEVANLGHVIAKLKKVNPEGLMAVDLVRSY
                     VEPILKNKKKAELIKAKMAGTSLEAIAKATGTSVQQATAVTMENPVLTGGVGQEPKVV
                     GNAFALTAGKLSAPIEGNTGVYVVKNISTAKAPVLKDHTAYVAKLKAQSAGDLNRVLP
                     ALKDKAEIKDNRKQFNY"
     gene            complement(7404..8663)
                     /locus_tag="PLQ70_05225"
     CDS             complement(7404..8663)
                     /locus_tag="PLQ70_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQE34050.1"
                     /translation="MEISIIILCLILCAFFSGMEIAFISSNKIYLELEKKQDNFISKT
                     LSKLTENPSKFIAAMFIGNTIALVIYGFLMSNLLLAWLGNLGFYFSGFLTIFLQILFS
                     TILILATAEFLSNVFFPIYANSLIKFFAIPAYGFYLLFYYISSFFIRISDFILIRFFK
                     TQGDQVSLYFSKAELGNYITEQMSTVENDDEMDSEIQMFQNALDFSGVKARDIMTPRT
                     EIVATDVFDSVTELKDLFIETGYSKVLVFQNSLDDIVGYVHSFDLFKKPKNIKSILIP
                     VEFVPETIFIKDAMNLLTKKRKSVAVVLDEYGGTSGIITIEDIIEELFGEIEDEHDSD
                     EELTEKELEDGVFVFSTRLDVEYLNQTYKLTIPESDSYGTLGGFIVDSTKEIPQKGDV
                     ITIGIYHFIIEEATDKKIELVKMTIKE"
     gene            complement(8666..8860)
                     /locus_tag="PLQ70_05230"
     CDS             complement(8666..8860)
                     /locus_tag="PLQ70_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34051.1"
                     /translation="MQYIKYTPFLYLILAAAFFYDAFTKWNLPNETPKISLLIGGLAV
                     FMFFFRRRFAKKLEDRNKKS"
     gene            complement(8872..9396)
                     /gene="lptC"
                     /locus_tag="PLQ70_05235"
     CDS             complement(8872..9396)
                     /gene="lptC"
                     /locus_tag="PLQ70_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQE34052.1"
                     /translation="MLVTCVVVALFVGCESNFKEVQKSNFSEFVPSGEAEKINLKYTD
                     SGRITAILVSPKMMEYATVDFPFTDFPKGIDVTLYDKNKKRTIILADYATSYKLTNII
                     DLRGHVKISSQDGQILETDQLFFDQKNEWFFTENNFTFTDPKGSSKGKGIDFSKDFKV
                     INSQKISGEIESDD"
     gene            complement(9478..>10111)
                     /locus_tag="PLQ70_05240"
     CDS             complement(9478..>10111)
                     /locus_tag="PLQ70_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34053.1"
                     /translation="MMYQYKLKEKLQFFSSLYYTPKSTLVSNNERIIGTVSYNSNYDL
                     STVDTFDPVITKTDLQLPQKWSLGLGIGNAKKWVAGGEVSIQDVGKLYNNYNTLSNVT
                     YGKYQRYSVGGYFTPNANPFMSYAKRITYRGGFVFEKTGLIVNSTSINDVGMTLGLGF
                     PITGSLSNLNLGLEFGKKGTTSNNLVQENYFIFNIGFSLNDKWFVKSKYN"
BASE COUNT         3284 a         1779 c         1548 g         3500 t
ORIGIN      
        1 aaactcccaa ccatagaaga aatatttggt ttgaagaatc catttccaaa aataataaca
       61 aacagtccta accaaaaaat agctttcgaa aaatctagat tactatcata aatagtagca
      121 ctgtaaaata gtgaaaactg accaatcgcc atcaaactac caccaagcaa gatacaactc
      181 ctgtttccta aatatttatc tgaaataaaa cctcctaata gtggtgttaa ataacaaaga
      241 gctagaaaac ctccatatat aattgatgca tttgactctc ctaacattag ggcatttacc
      301 ataaataatg ttaggattgc tctcattcca tagaaattga aacgttccca catttctgtt
      361 ccaaataata cccaaagtcc ttttggatgt cctgttttta ccgctgcttc actcatatta
      421 tttattttgg ttaaggtttt tattataaat tatttttgat aaaattagtc attttattga
      481 acaattgaat tcttgttttt cctccgtaaa ttccgtggtt tttatcggga tatatttggg
      541 aatcaaattg cttgttggct tgaattaaag cttccatcat ttgcatcgaa ttttgcacat
      601 gaacattatc gtcacccgat ccgtgaatca acaaatactt gccttttagt ttttctacaa
      661 aatttatggg tgaattatta tcatatccac taggattttc ctgcggtgtt tgcatatatc
      721 tttcagtata aacactgtca taaaaacgcc agttagttac tggagccact gcaattgcca
      781 tcttgaaaac gtcattacct ttcagaatac agttgctaga cataaaacca ccatatgacc
      841 aaccccaaat tccgattcga gatttatcga cataaggata atttccaatt actttagcgg
      901 catcaatttg atcttcgact tcgtattttc ctaattcttt ataagtcact tttttaaagg
      961 cagctccttt ataacccgtt cctcgaccat caacacaagc tacaatatag ccttgttgtg
     1021 cgagcattgc aaaccaataa tcgtcatagc tcccccactc attatttact tactgtgaac
     1081 ctggtccaga atattgatac ataaaaacgg gatatttttt agaagaatca aaatcttttg
     1141 gtttcattat ccaagcattt aattcattgc ccttttccgt ttttagaaca aaaaattctt
     1201 tttgcggcaa attgtactct tttaatttta tcgccaaagc ttcgttgttt tctattactt
     1261 gaagttgttt tccagtttta gattcgttca atgaataaat agttggctgt gaagcggtcg
     1321 aaaaagtatt aataaaatat tgaaaatttg gactaaaagt agcagcgtta gtgcctaata
     1381 tttgtgataa acgggttttg ttttttccat ccaaacccat tctaaaaaca gccctattga
     1441 tagaaccctc ttcagtcgat tgataaaaaa ccgacttggt tttctcatca aaaccataat
     1501 attcggtcac ttcccaattg cctttggtaa tctgattgat taattttcct gatttatcat
     1561 accaataaat gtgattgaaa ccatctagtt cagaagtcca aatgaaactg ttgtcgttca
     1621 ggaaagtcaa attatcttta tcaacaaaat caatatacga cttatctttt tcgttcaaga
     1681 ccagttttgt tgcggcagtt gttccatcta caaacaacaa gtctagatta tcttgatggc
     1741 gatttaatag ttttgcagac aaaacattag catcatttgt ccattcaatt cttggaatat
     1801 aaaaatcaga ataggtgctt aaatcaactt tttttgtggt tttggaatca acattatata
     1861 aatgcaaaga aaccaatgaa ttcttttcgc ccgctttcgg atatttgaaa gtttctattt
     1921 ttggatacaa atctttagaa aaaatcgaca ttgaaaattc tggaacttga ctttcgtcaa
     1981 aacggatata ggctattttt ttgctgtctt tactccaatc gaaagctctt acaaacgcaa
     2041 attcttcttc ataaacccaa tccgtaatac catttattat ggcatttttc tttccatctg
     2101 tagtaatttg agtatttgtt tttgtagcta tatcaaaaat ataaatgtta ttttctttga
     2161 cataaccaat tttttttccg tcaggcgaaa aagtaggctc ctgaacttgt tcaagaattt
     2221 tattcaattc tttcgtttcg agattgtaca gaaaataatc ggcagtaaag gaatgacggt
     2281 aaattcgatt agaattattg gcaatcaaca gctgtttttc gtctgaacta aacatataac
     2341 tatcaattcc gtcggataat ttgggctgat ttttggtctc aatcagggta tttacttttt
     2401 ttaaagtagc aaaatcaaat acatcaattt gcaaacttcg actggattgg tcataattta
     2461 aaactgtata ttgattggta tttttcatcg actgcaattc atccatctgt ttggcttgaa
     2521 attgcccagt ataaatggct tcgacggaga ctttttgttg tgcaaaaaca gaagttaaca
     2581 gcaacaaaaa cagcaggtta atcttatttg aaatcattaa ttatttatta atttaaaagt
     2641 ccccaatttt aatgaaaatt ttacaattat ttcacgtttc gactgttaaa tgttaaaaaa
     2701 atatacgatt taaccttttt tgagtacaaa tcgaatctga ataaaactac taaaaccgta
     2761 tctttgcact ctatcatccc ataatcaatt gagaatgaat aacgcagttg ctggattttc
     2821 taaattatcc aaagaagaaa aaataaattg gattgccaaa gaatactttt ctagtccgca
     2881 agaagctctt ggaattctaa aaaaatattg gaattcggac gaaaaattac aaaaacttca
     2941 cgatgaattt atcgaaaata cgataagcaa tttctatatt ccgcttggtg ttgctcccaa
     3001 ttttctgatt aacggaaaat acaatagcat tccgatggcg attgaggaaa gttcggtagt
     3061 agcggcagcc tcaaaagcag caaaattttg gtccactcgt ggtggattta aggctagagt
     3121 tctcaatact gaaaagattg gtcaagttca ctttatttac aaaggcgacg aatcgaaatt
     3181 agaatctttt tttgctataa ttaaagctaa atttttctcc aacacccaaa acattaccaa
     3241 aaacatgcaa aaacggggtg gtggaattct ggatattgaa ctaaaagtca aaacgcattt
     3301 actacccaat tactatcaat tgcacgccac attcgacact aaagattcga tgggcgccaa
     3361 ttttatcaat tcgtgtttag agcaatttgc caaaacactc caagaagaag cgcaactttg
     3421 ggatttgttt tcggaagaag aaaaaaacat cgaagtggta atgagtattc tttctaatta
     3481 tgttcccaat tgcatagttc gtgccgaagt ttcttgccca gtcgaagatt tagtagaaaa
     3541 acacattcca aatccaaaag catttgcaga gcgattcatt cgagcggttc aaatcgccga
     3601 agtagaacct tttcgagccg taacccacaa caaaggaatt atgaacggaa ttgatgctgt
     3661 tgtgcttgct acaggcaatg attttcgtgc cgttgaagcc ggaattcacg cttatgcttc
     3721 gagaaaaggt agctattcga gtttgtcaca cgccaaaatc gaaaatgata tttttacttt
     3781 ttggttagaa gtccctttgg ctttaggaac tgtcggcgga ttgacttcct tgcatccatt
     3841 ggtgaaattt tctcttgaaa tgcttgaaaa tccatcggcc gaagaattga tgcagtttgt
     3901 ggcggtggcg ggtttggcac aaaattttgc tgctttgcgt tccttgacca caactggaat
     3961 tcaagacgga catatgaaaa tgcacctgaa taatattctg aaccaatttg aagccacaga
     4021 agacgaacgc catttgattc acaaacattt caaacatcaa gtagtttcac acagctctgt
     4081 ggtagaatac attgaaactt taaggaattc tgaacaattt agactttaac ctgcaacaca
     4141 atcctatttt agttttttga gagctaaatg gattatttta ccaaatgaaa aaagaatttt
     4201 acagcaacgg aaaactatta atcactggag aatatctagt tcttgacggt gccaaagctt
     4261 ttgctttacc caccaaattt gggcaaaaac tgattgtcga gaaaggaaaa catccaggaa
     4321 taaaatggac tagttttgac gctgacggta gtaaatggct tgatttacaa gtttcattcg
     4381 aagatatttt aaataaaaat acgctcgttg aatcggagtc agtaaaaaat accttgattg
     4441 aaattttgca tcaaggttat ttattaaacc caaatttcat tgaaaatagt gacggttttg
     4501 ctataactac tcaattgact tttcctagaa attggggttt aggcacttcc tcaactttaa
     4561 tcaacaatat tgcgcaatgg atagaagtag atgcctttgt tttattgaat aatagttttg
     4621 gcggtagtgg ctatgatatt gcttgtgctc aaaatgacag tcctattttg tatcatttga
     4681 ataatggaaa acccataatc gaaaaagtac aatttgagcc taagtattct gaaaatttat
     4741 attttgttta tttggagaaa aagcaaagta gcaaagcttc tattgtttct tattacaaca
     4801 ataaaaatga aggattagac caaaagattg tcgaaaacaa taagataact tcagccgttt
     4861 tgaaagcaaa aacccttgca gattttgcaa tagctcttga agaacacgaa gctgaaatga
     4921 gtactattct agaaatgcaa accataaaag aatcactatt cccagatttt gacggagtaa
     4981 taaaaagtct tggtgcttgg ggtggcgatt ttgtattggt attatctgat gaaaatcctg
     5041 tagaatattt taatgaaaaa ggatttaaaa ctattgtttc ttataaggat atgattctat
     5101 aattattacc ctttattctt tttgaaataa gaataaaaaa atcccgttta gtttattaac
     5161 caaacgggat ttattattta caaaaacgtc aattctaata attgaattgt tttctgttgt
     5221 ctttgatttc agctttgtct tttaaggctg gcaatactct gtttaaatct ccagcacttt
     5281 gagctttcaa tttggctaca taagcagtat gatcttttaa aactggtgct tttgcagtac
     5341 taatgttttt cactacataa actcctgtat ttccttctat tggagcagaa agtttaccag
     5401 cagttaatgc aaatgcattt ccaactactt ttggttcttg acccacacca ccagttaaaa
     5461 cagggttttc cattgtaact gctgtagctt gttgaacaga agtacctgta gctttagcaa
     5521 ttgcttctaa tgaagttcct gccattttcg ctttgattaa ttctgctttt ttcttgtttt
     5581 ttaggattgg ttctacataa gaacgtacta aatctactgc catcaatcct tctggattta
     5641 cttttttaag tttagcgatt acgtgaccta agttagctac ttcaaatctt ttgatagcac
     5701 caacatccgt tccgtcttcg aaagcccatc taacaatatt tctttggtta cccaatggtc
     5761 caaatgattc gtccattaat tttgcagtta ctgctggcga aactgtaagt tttaatgctg
     5821 cagctgcttt tgcaaaatct ttagcgttag catcaatttc aaattgagtc gcttgagtaa
     5881 atactttatc agaagtagct tcagaagctt ctactttttg agctacagtt gctaaacgaa
     5941 cgccgtcttg tttgtctgta attttaataa tatgatatcc aaaaggagtt tctactaatc
     6001 caacttttcc aattggatta ttgaaaacaa agtcattgaa tggctttacc atttggttcg
     6061 gaccaaaaaa acctaaatca ccaccttgtt gtgcagatga atcatcagag gctgtgaaag
     6121 ccaacatcaa gaagctattt ggatttgcat ttacttgagc taaaatttct tcggcttttg
     6181 ctttggcttg ttctttagtt cttttctctt tttgatttgg aacttgagtt ccttcgtagc
     6241 taattaggat gtgacttgct tttgcattta ccccaacttt tctacctaaa gatttagata
     6301 aacaatagta ttttccgaac ttatatggac cgtagatttc acctggagcc aagttgtata
     6361 atttatctgc atcaatagct ggtaaatctt ttttggcgac ataagtagaa tcataaggaa
     6421 catctgaatt tgagtttaca aattcaacca cgtttgttgc tgatctaaat cctggcaaag
     6481 tatcattttt tccagttttt gtattataaa ctacacttcc tgataacaaa ccgttgattt
     6541 ttgctttcac ttcagcttca tcttctggcg aagctttgtc ttcaatcaaa acgtattcga
     6601 tttcacgagt ttcatctgat ttgtattttt tttcattctt tttcatgaaa gctacaatat
     6661 catcatcaga tacttttaca tcactatctt ttatggtaga atataatcca gcaacgtatg
     6721 caaagttaac tttatcagct tccatttgat atttcagttt tccttcactt tcagttgtgt
     6781 aagaacccgc ttttatcatt gtagcataca tttgatattt tgcgttaagc tcagcatctt
     6841 tttctctgtc ttttacatat tgagcaattt cagggttagt tttggtagct tctttgaatt
     6901 tatctttatc aaaacgccct gctgaattca aaaacattgg gttttttcca atattttgat
     6961 ctgactctaa aatttgcatg atatggtcag cacctactct caaacctaat ttatcgaatt
     7021 cagaggttaa caaagcgatt gaaacttcct gatcccaaac tctgttggca gcagcagttg
     7081 gtgtaacacc ttgttggctt ttttcgacat tgctaacttt aactctaaaa tcctcgaatg
     7141 gaatatcttt tccgttaaca ctaccaactt catttgaact tttaccaaag ttacctttag
     7201 taaaaacatc ccctgcaata aatgcaaata aacaaaatcc aataactact atcaataatg
     7261 cggaacgttg tctaattttt tgtaaaactg ccatttctat ttttattaat ttttatattc
     7321 agtttgcgaa aatacaatta tctacttaat aattacaata gtcgctctat aatttacaag
     7381 aaaaattgat tttcaaaaaa aaatcattct tttattgtca ttttaaccaa ttctatcttc
     7441 ttgtcggtgg cttcttcaat aataaaatga tagattccaa tagtgattac atcgcctttt
     7501 tgaggaattt ctttggtaga atctactata aaaccgccca atgttccata agaatcactt
     7561 tcgggaattg taagtttgta ggtttgattt aaatattcga catccaatcg agtcgaaaaa
     7621 acaaaaacac catcttcaag ttctttttcg gtcaattctt catcagaatc atgttcgtct
     7681 tcaatttctc caaaaagttc ttcgataata tcttcaatgg taataatgcc tgatgttcct
     7741 ccatattcgt caagcacaac ggctacgctt tttctttttt ttgtgagcaa attcatggca
     7801 tctttaataa aaattgtttc gggcacaaat tctactggaa ttaaaattga cttgatattt
     7861 ttaggctttt tgaacaaatc aaaagaatgc acataaccca caatatcatc gagtgaattt
     7921 tgaaatacta acacttttga atagcctgtt tcaataaata aatccttcaa ttcagttact
     7981 gaatcgaata catctgtagc tacaatttcg gttctgggtg tcataatatc tcgagctttg
     8041 acacctgaaa agtcgagcgc attttgaaac atttgaattt cagaatccat ttcgtcgtca
     8101 ttttcaacag tgctcatttg ttcggtaata taatttccaa gttcggcttt gctgaaatat
     8161 aaagacactt gatcgccttg tgttttgaag aatcgtatta aaataaaatc agaaatcctg
     8221 ataaaaaacg acgagatata ataaaataaa aggtaaaatc cataagccgg aattgcaaaa
     8281 aacttaatta aggagttggc gtaaatggga aagaaaacat tggataaaaa ctcggctgtc
     8341 gccaaaatta aaattgttga gaaaagtatt tgcagaaaaa tagttagaaa acccgaaaaa
     8401 taaaacccta gatttcctaa ccaagccaat aatagattgc tcataagaaa cccgtaaatc
     8461 accaatgcaa tagtattgcc aataaacatg gcagcaatga attttgaggg attttcggta
     8521 agttttgaaa gggtttttga aataaagtta tcctgttttt tctcgagttc caggtatatt
     8581 ttattagaag aaataaaagc gatttccatc cctgagaaaa aagcacatag tattaaacac
     8641 aatatgataa tactaatttc cataatcagg attttttatt tctgtcttcc aatttttttg
     8701 caaatcttct tctgaaaaaa aacataaaaa cggctaatcc tccaataagc aaacttattt
     8761 taggtgtttc attaggcaaa ttccatttgg taaaagcgtc ataaaaaaat gctgctgcga
     8821 gaataaggta taaaaaagga gtgtatttta tgtattgcat aatgttacta tttaatcgtc
     8881 agattcaatt tcgcccgaaa ttttttggga attaatcacc ttaaaatctt tactaaaatc
     8941 gattcctttt ccttttgacg agccttttgg gtctgtaaat gtaaagttat tttcggtaaa
     9001 aaaccattca ttcttttgat caaaaaacaa ttgatcagtt tctaatattt gaccgtcttg
     9061 agaagatatt tttacgtgtc ctcttaaatc aataatattt gttaatttat aggaagtggc
     9121 ataatccgcc aaaataatcg ttcttttttt gtttttgtca tataaagtaa catcaattcc
     9181 tttagggaaa tcggtaaacg gaaaatcaac tgtagcatat tccatcattt ttgggcttac
     9241 caaaatagcc gtaatccgac ctgaatcagt gtattttaaa tttatttttt cggcttcacc
     9301 gctgggaaca aattcagaaa aattactttt ttgcacttct ttaaaattac tttcacatcc
     9361 aacaaacaat gccacaacaa cacatgtgac aagcataaag atagattttt ttttaaatga
     9421 agtcatttac tgaactaaca aattgtgtga aataaattcg aaatagaaaa tgctttttta
     9481 gttgtatttg ctttttacaa accatttatc gtttagcgaa aatcctatgt taaaaataaa
     9541 ataattttct tgaactaaat tatttgaggt agttcctttt tttccaaact ctaaaccaag
     9601 gttcaaatta gaaagtgaac ccgtaatagg aaatcctaat ccaagtgtca taccaacatc
     9661 atttatggaa gttgaattga ctattaatcc tgttttttca aaaacaaaac ctcctctata
     9721 agtaattcgt tttgcataac tcataaatgg atttgcatta ggagtaaaat atcctccaac
     9781 gctatatctt tgatattttc cataagttac attacttaaa gtattgtagt tattgtataa
     9841 tttgcctaca tcttgtatag aaacttcgcc acctgcaacc cattttttgg cgtttccgat
     9901 ccctaaacct aacgaccatt tttgaggcaa ttgcaaatca gttttggtaa ttaccggatc
     9961 aaaagtatca acagttgata aatcataatt cgagttatat gaaaccgtgc caataattcg
    10021 ctcattattg gaaaccaaag tgcttttggg ggtataatat aaactactaa aaaactgtaa
    10081 tttttctttt aatttgtatt gatacatcat t
//
LOCUS       DAOSNN010000072         9959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6762_length_9959_cov_5.834814, whole genome shotgun sequence.
ACCESSION   DAOSNN010000072 DAOSNN010000000
VERSION     DAOSNN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6762_length_9959_cov_5.834814"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /locus_tag="PLQ70_05245"
     CDS             <1..2058
                     /locus_tag="PLQ70_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HQE34054.1"
                     /translation="GIIICDTNGKIIQHIEHKKGLQNNTVLTSFIDNKNNLWLGLDNG
                     IDFINENSPFTYFGFSYGISTVYASVVHNGKLYVATNQGVFYHVWNTPFREDNFSLIE
                     GTTGQAWNIQVIDGQLICGNNNGALVIENNHSVRTLDNRGYFGFKKIPNHPNFIIGSN
                     YNGFAVFEKGANGWVFRNQIAGFKKPSNSFEMDNSYMWLKKDELIYQMGISSDFKKFS
                     TIKTYSNLSNTLKGIGSIQTLNHTVYFQTNNRFFRYSQDQDLFFEDQKITALFKDIPK
                     IYSLKQDSEGNIWYIFKESLGVLSKTSNGNYRNIVAPFSSLTGNLVNNYLSINTINPK
                     NIFIGLTNGLAHYDSQLLNNSVRKPTAFIRSFSFPGDTLMFGNSQNTFEKQKIPYKSN
                     RVKFTFSSPSYENIDNIEFSHQLEGFDDRWSNWSPITIKEYTNLREGDYKMNLKARNG
                     FGVQSEVTQIPFTISPPWYRHFVAYFCYIILIIAIIYYIRNRIKIKIRKNRYYETIEQ
                     RRLYLERESKIRKEQFDLEKEIERLKNDKLQTKILSKDKELVTNTLQIVKKNKILKGI
                     IHKLKDINVDSFDESTKFQFNKLNKSIVREVNTDKSWKDLEKHIKNVHFDFLKRLKEK
                     YPTISPRELDLSTYLLMNMSTKEIAEIMNISSGGVEVSRYRLRKKFGLNKKENLTGFL
                     MSI"
     gene            complement(2116..4101)
                     /locus_tag="PLQ70_05250"
     CDS             complement(2116..4101)
                     /locus_tag="PLQ70_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HQE34055.1"
                     /translation="MKHNLISRITLCGLLISGFYANAQKIILEVDAAKTITKIQPTMF
                     GLFFEDINFAADGGLYAEMIKNRSFEFEKPLMGWEQPNTKRLSLNQQSGFALPITVLE
                     NKTNPNFCRVQVNDDKGYALVNEGFRGMGVKKEAKYNLSLKAANHDGAIKKIIIQFID
                     KDKKVLGETSIVPTSNDWKSYSTQFTATQTEAKAKLKITFEGTGTIDLDMISLFPEDT
                     WKNRKNGLRKDIVQLLYNMKPGFLRFPGGCIVEGRTLADRYQWKNSVGEVENRKTRIN
                     RWNMEFPQKAAPDYFQSFGLGFFEYFQLSEDIGAAALPVLSCGMACQYNTGELTPIDE
                     IDPYVQDALDLIEFANGSEATPWGKLRSDMGHPKPFNLKYIGVGNEQWGPDYIERYKI
                     FEKAIKEKYPKITIVSGSGPSPDGEYFDYGMQELRKLNAELVDEHYYKSPQWFRDNAA
                     RYDNYDRKGPKVFAGEYAAHSTIDNKLITKNNWESAFSEAAFMTGLERNAEVVHLTSY
                     APLMAHAEAWQWAPDMIWFNNLEAYGSANYQVQKLFSLNKGTDLLNITKDGKPLTGQN
                     NLYASAVKDANTKEIIVKLVNTSPTPQEVAIDLKGSKLESKGTVITLTSPNLADENSF
                     ETPQKIIPTTSEYKLKGEKAQMNLPAYSITVLKLKMK"
     gene            complement(4228..5181)
                     /locus_tag="PLQ70_05255"
     CDS             complement(4228..5181)
                     /locus_tag="PLQ70_05255"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HQE34056.1"
                     /translation="MKKTLLLLITVLLSANSYSQKDTLNAPKAVLPDVYADPHIAVFG
                     KKFYIYPTTDGTDGWNATSFTCWSSNDLVKWESEKVILDLPKDISWAKERAWAPAIAY
                     KNGKYYYYFSANVNIGVAVSDKPTGPFKDALGKPLAPKGMLKGQMIDPMVFVDDDGSA
                     YLYFGQGNCNMVKLNDDMISYDASKIISIKPKGYNEGSFVFKRNGKYYLMWSEFDTRD
                     PRYSIAYATSDSPLGPFTKADGRTVLKREGVVKGAGHHSVVKVPGKDEWYIAYHRFRI
                     PDGNGYNRETCISPMRFDKDGNILPVDVFEIVKPVKVKGKK"
     gene            complement(5255..6562)
                     /locus_tag="PLQ70_05260"
     CDS             complement(5255..6562)
                     /locus_tag="PLQ70_05260"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HQE34057.1"
                     /translation="MKNIKYPFLSLILLMTTVVFAQNKTDKEKTIADAKALQIPEGYT
                     KNLHWNLLLDSIGTSGSTISWKSSKSDYISNDGKLLKRSSRKGKKVKVTMTATLTSGS
                     AKAKKSFDISVAYTDQELQGYLFTYFEGSGPRTSDEQVRFGVSADAVNWFALNNNQPI
                     LSSETISGTGGIRDPHILRSEDEKSFYMVATDMNTAKNGWGHNPGIVMLQSDNLTDWK
                     HHFIDLEKSYPIKFANIQWVWAPQTIYDPAADKYMVYFTVKYKDDLKLDFYAAYANKD
                     FSGFENEPKLLFNPKYGAIDGDIIYKDGTYHFFFKGNTKDENGKEFKNGIRQAIGKSL
                     QGPWTENFNYLDAYSDRKIVVEGSSIFKLNQSDSYILMYDLYTNHRYEFQRSKDLYQF
                     TPAPESFTKNFYPRHGSVIGITAEEAKRLNEKWGGVPENLLKN"
     gene            complement(6580..8952)
                     /locus_tag="PLQ70_05265"
     CDS             complement(6580..8952)
                     /locus_tag="PLQ70_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HQE34058.1"
                     /translation="MIPNTIKKSLWLGIILVSTTTIFGQSAKLESFPLSSVRLLESPF
                     KKAQQTDLKYILSLDADRLLAPYFKEAGLETKAINYPNWENTGLDGHIGGHYLSALSE
                     MYAATGNQQIKERLDYMLNCFEKCQQKNGNGYIGGVPGSKELWADIAKGKIDAGSFSL
                     NKKWVPWYNIHKVYAGLVDAYNLTGNEKVKKMLIQFSDWCLTLTANLSDAQIQEILRS
                     EHGGMNEVFADVAAITGDKKYLTLAQKFSHKTILDPLLENKDVLNGMHANTQIPKVIG
                     FMRVAEVSGDKNWANAANFFWNTVVNNRTISIGGNSVREHFNPATDFSSMLESREGPE
                     TCNSYNMLKLSKHLFLANPSSKYMDYYERTTYNHILSSQHPDGGFVYFTPIRPRHYRV
                     YSEAQQSFWCCVGSGLENHGKYGELIYAHDQQNLYVNLFIPSTLNWKEKGISLTQNTK
                     FPFEEQSSIILTLKKAQQFAIKFRYPSWVENGQLKIKVNNQEMNLVKDQNSYVSIERK
                     WKSGDVISILLPMQNKTEQLPDKSDWVSFVRGPIVLAAITDTTDLKGLTADDSRMGHI
                     ASGAIYPIEDAPLLVSNNQHLASSLKAVAKKPFTFSASDLMYQEKYKELQLVPFFQIH
                     DARYMLYWPYTTKEKLPEIQKAMKEREEAQMQLEAITLDVVTAGEQQPESDHNFKGEK
                     TDTGTFKERHYRNGKGWFSYDLKNNDLEAKNLRITYFGADKNKNFDVFVNEILVASIN
                     MEGTEGNQFIDKIIELPTEILNAKNKILQVQFKAKPNSSITGIYEVRLLK"
     gene            complement(8958..>9959)
                     /locus_tag="PLQ70_05270"
     CDS             complement(8958..>9959)
                     /locus_tag="PLQ70_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HQE34059.1"
                     /translation="VLAQKTKAPKEKDMGAYLMVYFKDDTHGLYMALSKDGYSFTDVN
                     NAKPIIAGDTIAEQKGIRDPYIYRAPDGMFYLALTDLHIYAQKAGYRTTEWERDGKQY
                     GWGNNRGLVLMKSPDLIHWSHNVVRVDKDFPELAGIGCAWAPELIYDEKEQKTMIYFT
                     MRFGTEKEKVYYSYMNKDFTKLETLPKLLFEYPKNITYIDSDITKIGNKYHMFYVSHD
                     GTPGIKQAVSDTLNSGYKYDDKWYDPEPVCCEAPTIFKRIGEEKWVLIYDIYCINPHN
                     FGFSETSDFVNFKDLGHFNEGVMKTTNFSSPKHPAVIQITKKEAQKLAKKWNCEIKF"
BASE COUNT         2951 a         1903 c         1807 g         3298 t
ORIGIN      
        1 ggaattataa tttgtgatac caatggcaaa ataattcagc atattgaaca caaaaaagga
       61 cttcagaaca acactgtttt aacttctttt atagataaca aaaacaatct ttggttgggt
      121 cttgataatg gaattgattt tataaatgaa aattcacctt tcacctattt cggttttagt
      181 tatggtatta gtacggttta tgcttctgtt gttcataacg gaaagttgta tgtggccaca
      241 aatcaaggtg ttttttatca tgtatggaac accccttttc gagaagataa tttttcatta
      301 atagagggaa caacagggca ggcttggaac attcaggtaa ttgatggaca gttaatttgt
      361 ggaaacaata acggagcttt agtaattgaa aacaaccatt cagtcagaac tcttgacaac
      421 agaggttatt ttggattcaa gaaaatacca aaccatccca attttattat aggctctaat
      481 tacaatggtt ttgctgtttt tgagaaagga gctaacggat gggtattccg aaatcaaatt
      541 gccggtttta aaaaaccatc taactcattc gaaatggaca actcttatat gtggttaaaa
      601 aaggatgagt taatttacca aatgggtatt tctagtgatt ttaaaaaatt cagtaccata
      661 aaaacgtact ccaacttatc gaatacactt aaaggaattg gtagtattca aacactaaac
      721 cacactgtat attttcaaac aaacaatcgt ttctttagat attctcaaga ccaagatttg
      781 ttttttgaag accaaaaaat tacggcatta tttaaagata ttcccaaaat atattctttg
      841 aaacaagatt cagaagggaa tatctggtat atttttaaag aatcattggg ggttttgtca
      901 aagacttcta acggaaatta tagaaatatt gtagctccat tttcgagctt aacgggtaat
      961 ttagtcaaca attatttgtc tattaacacc attaatccaa aaaatatttt catcggacta
     1021 acgaatgggt tggcacatta tgattctcaa cttttgaata attcggttag aaaacccacc
     1081 gcattcatcc gaagtttttc gtttccagga gacacgctga tgtttggcaa tagtcaaaac
     1141 acatttgaaa aacagaaaat tccatataaa tcaaatcggg taaagtttac tttttcttct
     1201 cccagctatg aaaacataga caatattgaa ttttcgcatc aattagaagg cttcgatgac
     1261 cgatggagta actggtcacc cattactatc aaagaatata caaacttaag ggaaggtgac
     1321 tacaaaatga atctgaaagc aagaaacggt tttggagtac aatctgaagt aacacaaatt
     1381 ccatttacca tttctccgcc ttggtacaga cattttgttg cttatttttg ttacatcata
     1441 ctaattattg ccatcattta ttatataaga aatagaatta aaataaaaat acgtaaaaac
     1501 agatattacg aaactatcga acagcgaagg ctttatctgg aaagagaatc caaaattaga
     1561 aaagagcaat ttgatttaga aaaagaaatc gaacgattaa aaaatgacaa gcttcaaact
     1621 aaaatcctat caaaagacaa ggaattggtt actaatacgc tacaaattgt aaaaaagaac
     1681 aaaatactaa aagggattat tcataaactc aaagacatta atgtagattc ttttgacgaa
     1741 tcgactaaat ttcaatttaa caaactcaac aaaagtattg taagagaggt aaatacagat
     1801 aaaagttgga aagatcttga aaaacacata aaaaatgttc atttcgattt cctgaaaaga
     1861 ttgaaagaaa aatatccaac catttcccct cgagaattgg atttatcgac ctatttactg
     1921 atgaatatgt caacaaaaga aattgcagaa atcatgaaca tttcgagtgg aggtgtagaa
     1981 gtgtcccgtt atcgtttacg caaaaaattt ggtctcaata aaaaggaaaa tctaactggt
     2041 tttttaatga gtatttaacc caaaaaggcc atcatttctg atagcctttc ttgaaaaaaa
     2101 attatacaaa aaatattatt tcattttcaa cttcaaaaca gtaatcgaat aagctggcaa
     2161 attcatctgt gctttttcgc ctttcaattt atactcgctt gttgttggga taattttttg
     2221 gggagtttca aaagaatttt cgtctgccaa attcggactg gttagagtga taacagttcc
     2281 tttagattct aatttgcttc ctttcaaatc aattgcaact tcttgtggag tgggtgaggt
     2341 gttgaccaat ttcacaatga tttctttggt attggcatct tttacggcag aagcgtagag
     2401 gttgttttgt cccgttagcg gttttccgtc tttggtgatg ttcaataaat cggttccttt
     2461 gttgagagaa aacaactttt gcacctgata attcgcggaa ccataagcct ccaaattgtt
     2521 gaaccaaatc atatccggcg cccattgcca agcttctgcg tgagccatca acggagcata
     2581 agaagtcaag tgaaccactt cggcatttct ttctaaacct gtcataaaag cggcttcaga
     2641 aaaagcagat tcccagttgt ttttggtaat taacttattg tcaatagtac tgtgggcggc
     2701 atattctccc gcaaaaactt taggtccttt tcggtcgtaa ttgtcataac gcgcggcatt
     2761 gtccctgaac cattgcggac ttttataata atgttcgtcc accaattcgg cattcaattt
     2821 tctcaattcc tgcattccat agtcaaaata ttctccgtct ggcgatggcc cacttccaga
     2881 aacaatcgtg atttttggat atttttcttt tatcgctttt tcgaaaattt tatatctttc
     2941 gatgtaatca ggtccccatt gttcgttacc cacaccaatg tatttcaggt taaacggttt
     3001 tggatgtccc atatccgagc gcaatttccc ccaaggcgtc gcttccgaac cgttggcaaa
     3061 ttcaatcaag tccaaagcat cttgaacgta cggatctatt tcgtcaattg gcgttaattc
     3121 gccggtgtta tattggcacg ccattccgca actcaaaact ggcaaggctg ctgcgccaat
     3181 gtcttcggaa agttggaaat actcaaaaaa tcccaatcca aaactttgaa aataatcggg
     3241 tgccgctttt tgcggaaact ccatattcca acggttgatt cttgtttttc tattctcgac
     3301 ttcgcccacc gaatttttcc attgataacg gtctgccaaa gttcttcctt cgacaatgca
     3361 accgcctgga aaacgcaaaa atcctggttt catattatac aaaagttgca caatatcttt
     3421 gcgtaagccg ttttttctgt tcttccaagt gtcttctgga aacaatgaaa tcatatccaa
     3481 atcgatagtt cccgtacctt caaaggtgat tttgagtttg gcttttgcct cggtttgtgt
     3541 cgccgtgaat tgtgttgaat aggatttcca atcattcgat gtgggaacaa tactggtttc
     3601 gcccaatact tttttgtctt tatcgataaa ctgaataatt atttttttga tggcaccatc
     3661 gtgattcgcc gccttcaaag aaagattgta tttggcttct tttttaacac ccattcctct
     3721 aaatccttcg tttacgaggg catatccttt gtcatcattt acctgaactc tacaaaaatt
     3781 aggattggtt ttgttttcca ataccgtgat gggcaacgca aaacccgatt gctggttcaa
     3841 agaaagtctt ttggtattgg gttgttccca tcccatcaag ggtttttcga attcgaaaga
     3901 gcggttttta atcatttcgg catacaaacc tccatcggcg gcaaaattga tgtcttcaaa
     3961 aaacaaaccg aacatcgtgg gttgtatttt ggtaatggtt ttggctgcat ccacttccaa
     4021 aattatcttt tgggcattgg cataaaagcc ggaaattaat aaaccacaaa gggttattct
     4081 tgaaataaga ttgtgtttca tatttttatt cttgattatt agatgggatt ttttgcatac
     4141 aaaaatacgg atagcattag aaaatgaaat ggatgattac acctcaaata tggatagata
     4201 tgaattaaaa gatggattat ctgactacta tttcttcccc ttaaccttaa cgggtttcac
     4261 aatttcgaaa acatcgactg gtaaaatgtt tccatccttg tcaaatcgca ttggcgaaat
     4321 acaggtttca cggttgtaac cattaccgtc aggaattcta aagcggtggt aagcaatata
     4381 ccattcgtct ttgccaggaa cctttactac tgaatgatgt cccgcacctt tgacaacgcc
     4441 ttctcttttt aaaacagtac gaccatcggc tttggtaaaa gggccaagag gcgaatcgga
     4501 agtggcataa gctatggaat atctagggtc acgagtgtca aattctgacc acatcaggta
     4561 atattttccg tttcttttga atacgaaaga accttcgtta tagccttttg gtttgatgga
     4621 aataattttc gacgcatcat acgaaatcat atcatcattc aatttaacca tattgcaatt
     4681 gccttgtcca aaatacaaat aagctgaacc gtcatcatct acaaaaacca ttgggtcaat
     4741 catctgtcct tttagcattc cttttggtgc taatggtttt cccaaagcat ctttgaaagg
     4801 tccagttggt ttgtcggaaa ctgctactcc aatatttaca tttgctgaaa aataatagta
     4861 atattttccg tttttgtaag cgatggctgg tgcccaagct cgttcttttg cccagctaat
     4921 gtctttgggt aaatccaaaa ttactttttc ggattcccat tttaccaaat cattggaaga
     4981 ccaacaagta aaactcgtag cattccatcc atcagtacca tcggttgtag gatagatgta
     5041 gaattttttg ccaaaaaccg caatatgagg atcggcataa acgtctggca agactgcttt
     5101 tggtgcgttc aaggtgtcct tttgagaata tgaatttgcg gaaagcaaaa cagtgattag
     5161 tagtaagagt gtttttttca tttcattttt cattataaag tttaatagtt ctgctggaaa
     5221 cggaacaatt atttcttaat ggtttaaaat tgtttcagtt ttttaataaa ttttctggaa
     5281 ctcctcccca tttttcgttc agtcgtttgg cttcttcggc agttattcca atgacgcttc
     5341 cgtggcgggg atagaaattt ttggtaaatg attctggtgc tggcgtaaac tggtacaaat
     5401 ctttgctacg ctgaaactca taacggtgat tcgtatataa atcatacatc aaaatatagg
     5461 aatccgattg gttcaatttg aaaatgctcg atccttcaac cacgattttt ctatcggagt
     5521 aagcgtctaa atagttgaaa ttttctgtcc aagggccttg aagcgatttt ccaatagcct
     5581 gacggattcc gtttttgaat tctttgccgt tttcgtcctt ggtatttcct ttgaaaaaga
     5641 aatgataggt gccatcttta taaataatat cgccgtcaat agctccatat ttgggattga
     5701 atagcaattt aggttcgttc tcaaaaccgc tgaaatcttt gttggcgtaa gctgcataaa
     5761 aatcgagttt caaatcgtct ttgtatttca ccgtgaaata caccatatat ttatcggctg
     5821 caggatcata aatggtttgt ggcgcccaaa cccattggat attggcaaac tttattggat
     5881 aacttttttc gaggtcaata aaatgatgct tccaatctgt taaattatcc gattgcaaca
     5941 tcacgattcc aggattgtgt ccccatccgt ttttggcggt attcatatcg gtagcgacca
     6001 tataaaaact tttttcgtcc tcgcttcgca aaatgtgtgg atctctaatt ccgcccgttc
     6061 ctgatattgt ttcggatgaa agaataggct gattattgtt caaggcaaac caattcacgg
     6121 catcggcact aactccaaaa cgaacttgtt cgtcgcttgt tcttggccct gaaccttcga
     6181 aataggtaaa caaataacct tgcaattctt ggtcagtgta ggctaccgaa atatcaaatg
     6241 attttttggc tttggcactt ccagaagtaa gtgtggctgt cattgtaacc tttacttttt
     6301 tgccttttct tgaagaacgt ttcagtagtt ttccatcgtt cgaaatgtaa tccgatttac
     6361 tggatttcca gctaatcgtc gaaccacttg tgccaatcga atcgagtaac aaattccaat
     6421 gcaaattttt agtatagcct tcggggattt gcaatgcttt tgcatcggca atagtttttt
     6481 ctttatcggt tttattttga gcaaaaacaa ctgtagtcat caaaaggatg agactcaaaa
     6541 aagggtattt aatatttttc atatttatgg aattttaatc tacttcaaca acctaacctc
     6601 ataaataccc gtaatactcg aattgggttt ggctttaaat tgcacttgca aaatcttatt
     6661 tttggcgttc aagatttcgg taggaagctc gatgattttg tctataaatt gattgccttc
     6721 cgttccttcc atattgatgg aagcaaccaa aatttcattc acaaaaacat caaaattctt
     6781 attcttgtcc gcaccaaaat aagtgatacg aagattttta gcttctaaat cattgttttt
     6841 taaatcataa ctaaaccatc cttttccatt acgataatga cgttctttga atgttccagt
     6901 atcggttttt tcgcctttaa aattatgatc cgattccggt tgttgttcgc cggcggtcac
     6961 tacatcaagg gtaatcgctt ccaattgcat ctgtgcttcc tctctttctt tcatcgcttt
     7021 ttgaatttcc ggcaattttt ctttagtggt gtaaggccaa tacaacatat atcttgcatc
     7081 gtgaatttgg aaaaacggca ccaattgcaa ctctttatat ttctcctgat acattaaatc
     7141 tgaagccgaa aaagtaaagg gttttttggc aacagctttt aaggatgaag ccaaatgttg
     7201 attgttcgaa accaataaag gtgcgtcttc tatcgggtaa atggcaccac tggcaatgtg
     7261 tcccattcgg ctgtcgtcgg ctgttaatcc ttttaaatcg gtagtgtctg ttatggcggc
     7321 aagcacaatt ggtcctcgaa caaatgatac ccaatcggat ttatcgggca attgctccgt
     7381 tttgttttgc attggcaaaa gtatcgaaat cacatcgcct gacttccatt ttcgttctat
     7441 cgaaacgtat gaattttgat ctttaaccaa gttcatttcc tgattgttga cctttatttt
     7501 caattgccca ttttcgaccc aagatggata acggaatttg atggcgaatt gctgtgcttt
     7561 ttttaatgtc aatatgatgg acgattgttc ttcaaacggg aatttggtgt tttgcgttaa
     7621 cgaaattcct ttttctttcc aattcaaggt cgaaggaata aacaaattca catagagatt
     7681 ttgctggtcg tgcgcataaa tcagttcacc atattttccg tggttttcca atccagaacc
     7741 cacacaacac caaaaagatt gttgcgcctc agaatacact cggtaatggc gtggtcgaat
     7801 aggtgtgaaa tagacaaaac caccatcggg atgttgtgac gacagaatgt ggttgtaggt
     7861 cgtgcgttcg taataatcca tatattttga agaaggatta gccaaaaata aatgcttgct
     7921 caatttcagc atgttgtaac tgttgcaagt ttcgggacct tcacgggatt ccaacattga
     7981 ggaaaaatcg gttgctggat taaaatgctc ccgcacgcta tttcctccaa tggaaatcgt
     8041 tcggttattg accaccgtgt tccagaagaa gttggcggca ttcgcccaat ttttgtcgcc
     8101 cgaaacttcg gcaacacgca taaaacctat tactttagga atttgagtat tggcatgcat
     8161 tccgttcagg acgtctttat tttccaaaag cggatccaaa attgttttgt gggagaattt
     8221 ttgggccaaa gtcaggtatt ttttgtcgcc tgtaatggct gccacatcag caaaaacttc
     8281 gttcattccg ccgtgttcgc ttcgtaatat ttcctgaatt tgggcatcag ataaattggc
     8341 ggtaagcgtc aaacaccagt ccgaaaattg gatgagcatt tttttgactt tttcatttcc
     8401 ggtcaaatta taagcatcca ccaatccggc atatacttta tgaatgttgt accaaggcac
     8461 ccatttttta ttcaatgaaa agctgccggc atcgatttta cctttggcaa tatccgccca
     8521 taattctttg cttccgggaa ctccgccaat gtatccgttg ccgttttttt gttggcattt
     8581 ttcgaaacag ttcagcatat aatcgagtct ttccttgatt tgctgatttc ccgttgcggc
     8641 atacatctcg gacaaagccg aaagatagtg tccaccaata tgaccatcca agcccgtgtt
     8701 ttcccaattg ggataattga tggccttggt ttccaaaccc gcttctttga aataaggagc
     8761 caaaagtctg tctgcgtcaa gcgataaaat atactttaaa tccgtttgtt gcgccttttt
     8821 aaatggactt tcgagcaatc gaactgaaga caatgggaaa ctttccaatt tagcggactg
     8881 tccaaaaata gtagttgttg aaactaatat aattccgagc caaagcgatt ttttgatggt
     8941 atttggtatc atatctttta aaatttaatt tcgcaattcc actttttagc caatttttgt
     9001 gcttcttttt tggtgatttg aatcactgcg ggatgtttgg gcgaagagaa atttgttgtt
     9061 ttcatcacac cttcgttgaa atgtcccaag tctttgaaat taacaaaatc cgaagtttcg
     9121 ctaaacccaa aattatgtgg attgatacaa taaatatcat aaatcagcac ccatttttct
     9181 tcaccaatac gtttgaaaat tgttggtgct tcacagcaaa ccggttcagg gtcataccat
     9241 ttatcgtcgt atttgtaacc cgaattcagg gtgtcggaaa cggcttgttt gatccctgga
     9301 gttccgtcgt gagaaacata aaacatatgg tatttgtttc ctattttggt aatgtcagaa
     9361 tcaatatagg ttatattctt aggatattcg aaaagtagtt ttggaagggt ttccaatttg
     9421 gtaaagtcct tgttcatata agaataatac actttttctt tttctgttcc aaagcgcatc
     9481 gtaaagtaaa tcattgtttt ttgctccttt tcatcataaa tcaactcggg agcccaagca
     9541 caaccaatgc ctgccaattc tggaaaatct ttgtcaacac gaactacatt gtgcgaccaa
     9601 tgaattaaat cgggcgattt catcaaaact aatccacgat tgttgcccca gccatattgt
     9661 ttgccatcac gttcccattc ggtggtgcga tagcctgctt tttgggcata aatgtgtaaa
     9721 tcggtcaatg ctaaataaaa cattccatca ggtgctcggt aaatgtaggg atcacggatt
     9781 cctttttgtt cggcaatcgt atcgcctgct atgatgggtt tggcattgtt tacatccgta
     9841 aaactgtaac cgtctttgct taaagccata tacaaaccgt gggtgtcgtc tttaaaatag
     9901 accattaggt aagcacccat atctttttct tttggtgctt tagttttttg tgctaaaac
//
LOCUS       DAOSNN010000073         9956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6765_length_9956_cov_4.973033, whole genome shotgun sequence.
ACCESSION   DAOSNN010000073 DAOSNN010000000
VERSION     DAOSNN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6765_length_9956_cov_4.973033"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLQ70_05275"
     CDS             <1..197
                     /locus_tag="PLQ70_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010177206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQE34060.1"
                     /translation="RQPGCSACLAMNDDKVPAGKYAVSTSNRNFEGRQGPGSRTMLAS
                     PIMAAAAAVTGKLTDPRELM"
     gene            325..921
                     /gene="leuD"
                     /locus_tag="PLQ70_05280"
     CDS             325..921
                     /gene="leuD"
                     /locus_tag="PLQ70_05280"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQE34061.1"
                     /translation="MAYDKFNILTSSAVPLPIENVDTDQIIPARFLKATKREGFGDNL
                     FRDWRYNGDDTPKADFVLNNKTYSGKILVGGKNFGSGSSREHAAWAVYDYGFRAVVSS
                     FFADIFKGNCLNIGVLPVQVSPEFSETIFKAIEADPNTQLEINLPEQTITLLATGQKE
                     SFDINGYKKNNMINGFDDIDYLQNIKGEITAFADKLPY"
     gene            1029..>1702
                     /locus_tag="PLQ70_05285"
     CDS             1029..>1702
                     /locus_tag="PLQ70_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQE34062.1"
                     /translation="MKKRKIEIMDTTLRDGEQTSGVSFSAAEKLTIAQLLLEELNIDR
                     IEIASARVSEGEFQGVKGIMSWASAKGYSNRIEVLTFVDGGLSIEWMKKTGAKVQNLL
                     TKGSLNHLTHQLKKTPEQHFSEIAQAIALANENGIATNVYLEDWSNGMRNSPDYVFQY
                     LDFLVTQPVKRILLPDTLGVLIPSDTFEFISKITSKYPNIHFDFHAHNDYDLSVSNVM
                     EAIKAG"
     assembly_gap    1703..1802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1803..2381
                     /locus_tag="PLQ70_05290"
     CDS             <1803..2381
                     /locus_tag="PLQ70_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HQE34063.1"
                     /translation="NLLPERFGRKRKYALGKTSGKANIEKNLQELGLHLNQEDLKLVT
                     QRIIELGDKKETVTKEDLPYIISDVLDSQAYEDKIVIESYVLVHSKGTRPSTTLCLKI
                     DGEIIEEHAQGDGQFDAFMNALTRIYKSKKLILPKLIDYAVRIPPGSSSDALCETIIT
                     WTNGKKEFKTRGLDSDQTVAAIKATQKMLNVI"
     gene            2574..3629
                     /gene="leuB"
                     /locus_tag="PLQ70_05295"
     CDS             2574..3629
                     /gene="leuB"
                     /locus_tag="PLQ70_05295"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQE34064.1"
                     /translation="MKFNIALLAGDGIGPEVINEAVKVSDAIAKKFNHEINWIPALTG
                     ACAIDAVGVPYPDETHEICVNADAVLFGAIGHPKYDNDPSAPVRPEQGLLLMRKKLGL
                     FANVRPTFTFPSLLDKSPLKRERIEGTDLVFLRELTGGIYFGEKGRKDGGDTAFDNCV
                     YTREEVQRLAKKGFELAMTRSKKLCCVDKANVMETSRLWRETVQAMEKDYPEVTVSYE
                     FVDAVAMRLVQWPNSYDVLITENLFGDILTDEASVISGSMGLMPSASMGAKVSLFEPI
                     HGSYPQATGLNIANPMATVLSAAMMFENFGLMEEGKAMRDAVNKALEAGIVTEDLADG
                     GKAYGTKEVGDWLAANI"
     gene            complement(3750..4091)
                     /locus_tag="PLQ70_05300"
     CDS             complement(3750..4091)
                     /locus_tag="PLQ70_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HQE34065.1"
                     /translation="MNIFVGSLPFSIEEADLRESFEAYGAVDSVKIITDKFTGRSKGF
                     GFVEMPSDEEAQKAIDELNGATVQGRTIVVNKSEPKPEGERRSFNNNRGGDSRGGYGN
                     NRGGGDRGGRY"
     gene            complement(4239..4721)
                     /locus_tag="PLQ70_05305"
     CDS             complement(4239..4721)
                     /locus_tag="PLQ70_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HQE34066.1"
                     /translation="MKKQFLYLFLLIAFISCTTDVKFNNPAFEGQKDNVFWRAIDTKA
                     SVDANGALTIIGYTRNEKLTLITDSKSPDTYILGVSDANAITYILTGASGTTTFSTGI
                     GIGEGQIVIEEYDAVNNTVTGTFKFNAENIYNNPLAGPILNFQYGHFYKIPVTTEFIN
                     "
     gene            4917..5483
                     /locus_tag="PLQ70_05310"
     CDS             4917..5483
                     /locus_tag="PLQ70_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQE34067.1"
                     /translation="MSVKIRLQRHGKKQKPFYWVVAADARSKRDGKYLEKIGTYNPNT
                     NPATIELNLDSAVKWLHNGAQPTDTAKAILSYKGALLKHHLDGGIRKGALTQEQADAK
                     LAAWLEAKAGKVDAKKDGLSKAQADAKAKALKAEQAVNAKRLADAAQAEADAIAAATP
                     AVEEEVAEVEAAAEEATEAPAAEETPEA"
     gene            5499..6026
                     /gene="rimM"
                     /locus_tag="PLQ70_05315"
     CDS             5499..6026
                     /gene="rimM"
                     /locus_tag="PLQ70_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466225.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQE34068.1"
                     /translation="MRKEDCFYLGKIAKKFSFKGEVLAYLDTDEPELYENLESVFVEC
                     NKHLVPFFIENSSLHKNDFLRIRFEDINTEDEADALIGNDLYLPLKMLPKLTGNKFYF
                     HEVIGFEVEDKRLGYVGEIQSINDTTAQPLFEVLNKDGAEILIPMIDHFLVKVDRENK
                     KIIMDLPEGLIEMYL"
     gene            6110..6817
                     /locus_tag="PLQ70_05320"
     CDS             6110..6817
                     /locus_tag="PLQ70_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQE34069.1"
                     /translation="MFQFKQFSVKQDRTAMKIGTDGVLLGAWTPLENNPFSILDIGTG
                     TGIIALMLAQRSAAEQIDALEIDEDAYEQATDNFENSPWNDRLFCFHAGLDEFVEEPE
                     DEYDLIVSNPPFYSEDYKSSNDQRDLARFQDAMPFEDLIEAAALLLSENGIFSVIIPF
                     KEEEKFLALANEYELFPLKITRIKGTPTSETKRSLLAFGRNETPNFPIDELIIETARH
                     IYTPEYIALTKDFYLKM"
     gene            6938..7630
                     /locus_tag="PLQ70_05325"
     CDS             6938..7630
                     /locus_tag="PLQ70_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34070.1"
                     /translation="MGKIDINFDFRLDSKCGDPDTDSQKLYDAHKLLWSKELPNGKMF
                     NCEIKGDSYGRLLIKNNLYMNLSSDRMCPHFDGKYKNKFEGWLSEIEIGELKHKVRTI
                     GGHIIFPAHKKNGFTVNQARGVSRIICDRFDLTLECIRRFYKDEQSPLYKTLINYKDF
                     FDSFIDFKGYVDFFLLQDFINNQEQIEFSLPFDNFTRPPLPQTIDEYKQYKTHIIDLV
                     NRRNKRILENYI"
     gene            7664..8632
                     /locus_tag="PLQ70_05330"
     CDS             7664..8632
                     /locus_tag="PLQ70_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HQE34071.1"
                     /translation="MKTNFSLFLTLFLTLGLLAQSPVISNKNFSFGTYGRAGIGYGLG
                     TTAEFPQSLNLNGMGSIGGRFEENDYLELATAMHFSPTNANKDITKINVQARFAFYTT
                     QGQLIGNVSNKSIGGITTAMPELFAEAKNLMGSDWSVWIGARLFRGDDIHIIDHYYFD
                     DHSGQGVGIQHKNTKFSVIFTGSIDTTSTLPPNFYLNIVNGTPTLGLRNRYISILEHE
                     IQVKKGYVKLMGEFQRLPAGEAKDANTKYNYPADYGFVLGAKYNKNISTKHPGSFNAV
                     SARYGTGIANGGDGGSSRTYMTYGAPNLETNNSRRRTQSPLPKLSY"
     gene            8629..9066
                     /locus_tag="PLQ70_05335"
     CDS             8629..9066
                     /locus_tag="PLQ70_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HQE34072.1"
                     /translation="MNLNEKYSLNGYAVYTKSKGASDSKDKAPDYLGKQMLFNRKQDI
                     SFGARGTYYINNWLHLLHELDFAWRKDGTQDFAQMTKFTIAPTLVPTAKRDVWERPHF
                     RFVYSVAHYNTFAAENLYSPYLAQSGTKNWGQYVGIKMEWWIW"
     gene            9153..>9956
                     /locus_tag="PLQ70_05340"
     CDS             9153..>9956
                     /locus_tag="PLQ70_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQE34073.1"
                     /translation="MKPDLFQAPDYYNVDELLTDEHKLVRDSAREWVKREVSPIIEDF
                     AQRAEFPKQIIKGLAEIGAFGPYIPEEYGGAGLDQISYGLIMQEIERGDSGVRSTASV
                     QSSLVMYPIWKYGNEEQRMKYLPKLASGEMMGCFGLTEPNYGSDPGSMITNFKDMGDH
                     YLLNGAKMWISNAPFADISIVWAKNEEGRIHGLIVERGMQGFSTPETHNKWSLRASAT
                     GELIFDNVKVPKENLLPNKSGLGAPLGCLDSARFGIAWGAIGAAMDCYDT"
BASE COUNT         3353 a         1722 c         1844 g         2937 t
ORIGIN      
        1 tgcgtcagcc aggttgttca gcttgtttgg caatgaacga tgataaagtt cctgccggaa
       61 aatatgcagt aagtacatca aacagaaact tcgaagggcg tcaaggtccg ggttcaagaa
      121 cgatgctagc ttctccaatt atggcagcag cggcagcagt taccggaaaa ttgacagatc
      181 caagagaatt gatgtagttg aatgtcaatt tcaatgacaa taacaatttc aaaaaagaat
      241 taaaaataaa aagattttaa ctatttatga tttatttctc ccaaaggaaa atctgaattc
      301 taaaatctaa aatctaaaat tacaatggca tacgataaat ttaatatcct taccagtagt
      361 gcagtgccac taccaataga aaacgtagat accgatcaaa tcattccagc tcgtttcttg
      421 aaagctacaa aacgtgaagg ttttggagac aatcttttca gagactggag atacaacgga
      481 gatgatactc caaaagccga tttcgtattg aacaacaaaa cttatagcgg aaaaatcctt
      541 gttggaggaa aaaactttgg ttcaggttcg tctcgtgagc acgctgcttg ggcggtttac
      601 gattacggat tccgtgctgt agtttcttct ttctttgcag atatcttcaa aggaaattgc
      661 ttgaatattg gtgttttgcc agtacaagtg agtcctgaat tttcagaaac tattttcaaa
      721 gccatcgaag cggatccaaa cacgcaattg gaaatcaatt tgccagagca aaccattact
      781 ttattggcta ctggacaaaa agaatctttt gatatcaacg gatacaaaaa gaacaatatg
      841 atcaacggct ttgatgacat tgattattta caaaacatta aaggagagat tacagcattt
      901 gctgacaagc ttccttacta ataaaaaaga ttcagccaga cctatgacgt ttttaaaacc
      961 tcatgggtct tgttgcgaaa taaataaaac gggttttcat atcagattgc tttagcctca
     1021 aatttaaaat gaaaaaaaga aaaattgaaa taatggacac gacgctccgc gatggtgaac
     1081 aaacctcggg agtatctttt tctgctgcag aaaaattaac cattgcacaa ttattattag
     1141 aagaattaaa tattgataga atcgaaattg cttctgcacg cgtgagcgaa ggagaatttc
     1201 aaggtgtaaa aggcatcatg tcttgggcat cagcaaaagg atatagcaat cgtattgaag
     1261 ttttgacttt cgtcgatggt ggactttcta tcgaatggat gaaaaaaaca ggagccaaag
     1321 ttcaaaatct tttgaccaaa ggttcactaa accatttgac tcatcaatta aaaaaaacac
     1381 ctgaacagca tttctcagaa attgcacaag ctattgcgtt ggccaatgaa aacggaattg
     1441 caaccaatgt ctatttggaa gattggagta atgggatgcg aaattctcca gattatgttt
     1501 ttcaatattt agattttttg gtgacacaac ccgtaaaaag aatcttgctg cctgacactt
     1561 taggcgtttt gattccttcg gatacttttg aatttatttc taaaatcaca tcaaaatacc
     1621 ccaatattca ttttgatttc cacgcacata atgactatga tttaagtgtt tcgaatgtca
     1681 tggaagctat aaaagcgggt atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnaatttgct tccagaacgt tttggaagaa aacgaaaata tgctttagga aaaacatcag
     1861 gcaaagccaa tatcgaaaaa aatctgcaag aattaggttt gcatttgaat caggaagatt
     1921 tgaaattggt tacccaacgc atcatcgaat tgggtgataa aaaagaaacg gtaaccaaag
     1981 aagacttgcc ttatatcatt tctgacgttt tggacagtca agcctatgaa gacaaaattg
     2041 ttattgaatc ttatgttcta gttcattcta aaggaaccag accctcaaca acactttgtt
     2101 tgaaaattga cggcgaaatt attgaagaac acgctcaagg cgacggtcaa tttgatgctt
     2161 tcatgaatgc tttgacaaga atatacaaaa gcaaaaagtt gatactgcct aaactgattg
     2221 attatgccgt tcgaattcca ccaggaagta gttcggatgc actttgtgaa accatcatca
     2281 cttggactaa tggaaaaaaa gaattcaaaa ctagaggatt agattcagat caaactgttg
     2341 cggctatcaa agcaacacag aaaatgctga atgttatata attaaaaaag tacctaagtt
     2401 gctaagaatc tgagttccta agtttttaaa aactttctta gaatcttaat aacgaatgac
     2461 ctttgaacct taaaaattaa aatcaataat taagagacta agttaccaag atttcagatt
     2521 ttcaacctta gaatcttagc aactcagaac cttagaacct taaaaacaaa ataatgaaat
     2581 ttaacattgc ccttttagct ggagacggaa tcggaccaga agtaataaat gaagctgtaa
     2641 aagtttctga cgctattgct aaaaaattca atcacgaaat caattggata cctgcattaa
     2701 caggagcttg cgcgattgat gctgtaggag ttccttatcc tgatgaaact cacgaaatat
     2761 gtgtaaatgc cgacgctgtt cttttcggag ctattggtca cccaaaatac gataatgatc
     2821 catcagcacc cgtaagacca gagcaaggat tgttgttgat gcgtaaaaaa ttaggattgt
     2881 ttgctaatgt acgtcctacc tttacatttc cttctttatt agacaaatct ccactaaaaa
     2941 gagaacgtat tgaaggaact gacttagttt tcttgagaga acttacaggt ggtatttatt
     3001 ttggtgaaaa aggaagaaaa gatggtggag acactgcttt cgacaactgt gtttacacaa
     3061 gagaagaggt acaacgtttg gccaaaaaag gttttgaatt agctatgaca cgttctaaaa
     3121 aattgtgttg tgtagataaa gcaaacgtta tggaaacgtc tcgtttgtgg agagaaacag
     3181 ttcaagccat ggaaaaagac tatccagaag ttactgtttc ttacgagttt gttgatgctg
     3241 tcgccatgcg tttagtacaa tggccaaact cttatgatgt attgattaca gaaaacttgt
     3301 tcggagatat cttaactgat gaagcttctg tgatttcagg atcaatggga ttaatgcctt
     3361 ctgcctctat gggagctaaa gtttcattgt ttgagccaat ccacggatca tatccacaag
     3421 caactggttt gaatattgct aacccaatgg ctacagtttt atctgctgcc atgatgtttg
     3481 aaaacttcgg attgatggaa gaaggaaaag caatgagaga cgctgtaaac aaagctttag
     3541 aagctggaat cgttacagaa gatttagccg atggcggaaa agcatacgga accaaagaag
     3601 ttggagattg gttagctgct aatatctaat ataattatta tgacgtagag acgttgcact
     3661 gcaacgtctc tacgtcataa taattataaa atacaacaaa aaaggtgtca attttcattg
     3721 acaccttttt ttatagaata gagaaaatat taatatcttc ctcctctgtc tccaccacca
     3781 cggttgttcc cataacctcc gcgtgaatct ccaccacggt tgttgttaaa acttcttctt
     3841 tcgccttctg gttttggctc tgatttattt accacaatag tacgaccttg aacagtcgct
     3901 ccgtttaact cgtctattgc tttttgagct tcttcatcac ttggcatttc aacaaaaccg
     3961 aatcctttac ttcttccagt aaatttatca gtaataattt ttacagaatc aactgcaccg
     4021 taagcctcga aagactctct taaatctgct tcctcaatac tgaacggaag acttccaaca
     4081 aaaatattca tatgtactta tttagtataa ttgtgacaaa tgtagtctta ttttcttcta
     4141 atccactaca cattaggtta tttgtgattt atatgtgatg aatattcaaa ataaagcttc
     4201 aaatatcttg attacaagag atttttaacg ttttgtcctt aattaataaa ttctgttgtt
     4261 acaggaattt tataaaagtg tccatattga aaattcaaaa tgggtcctgc caatggattg
     4321 ttataaatat tttcagcatt aaatttaaat gttccagtaa cggtattgtt tacagcatca
     4381 tattcttcga ttacaatttg cccttcacca attcctatac ctgtcgaaaa agtagttgtt
     4441 ccactagctc ctgtcaaaat ataagtaatt gcatttgcat cacttacacc taaaatatag
     4501 gtgtcagggc ttttagaatc tgtaattaaa gtcaattttt catttctcgt gtagccaata
     4561 attgtcaaag caccatttgc atcaactgag gcttttgtat caatggcgcg ccaaaacaca
     4621 ttgtctttct gcccttcgaa agcgggattg ttgaacttta catccgtagt acaagaaata
     4681 aaagcaatta ataggaatag atataaaaac tgttttttca taatcgagaa gtttgtcttg
     4741 acaaaagtaa attattttga tcaaaaaagt gaattacatc aggaaaatag cctaaaatag
     4801 tgtctttcaa atatttgaaa taattattag ttattaattg cccattgtta attaattttt
     4861 atctttgcac ccttaattaa tcgaggtcga gtacctcaaa atttaatcat tagattatgt
     4921 cagtaaaaat tagattacaa agacacggta aaaaacaaaa accgttttac tgggttgtag
     4981 cagcagatgc tcgctcaaaa agagatggta aatacctaga aaaaattggt acttacaatc
     5041 caaacacaaa ccctgcaact atcgaattga atttggatag cgcagtaaaa tggttgcaca
     5101 atggtgctca accaacagac acagcaaaag caattctttc ttacaaagga gccttattga
     5161 aacaccacct tgatggaggt attcgtaaag gagctttgac tcaagaacaa gctgatgcaa
     5221 aattagctgc ttggttagaa gctaaagctg gtaaagttga cgctaaaaaa gacggtttat
     5281 caaaagcgca agctgatgct aaagccaaag ctttgaaagc tgaacaagct gtaaacgcaa
     5341 aacgtttagc tgatgctgct caagcagaag ctgatgctat tgctgctgca actcctgctg
     5401 ttgaagaaga agttgccgaa gttgaagctg ctgctgaaga agctacagaa gctcctgctg
     5461 cagaagaaac accagaagca taattttaaa aggctaaaat gcgtaaagag gattgtttct
     5521 atttaggtaa aatagccaaa aaatttagtt tcaaagggga agtcctagcc tatttagaca
     5581 cagacgaacc cgagttatac gaaaacttgg aatcagtgtt tgttgaatgc aacaaacact
     5641 tggttccttt ttttattgaa aatagctcgc tacacaaaaa cgatttccta agaattcgtt
     5701 ttgaagatat aaatactgaa gatgaagccg atgctttaat tggaaatgat ttatatcttc
     5761 ccctaaaaat gttgcctaaa cttacaggca acaaattcta ttttcacgaa gtaattggtt
     5821 ttgaagtaga agacaaacgt ctgggatatg ttggagaaat tcaatccatc aacgacacta
     5881 cggcgcaacc cctttttgaa gttctcaaca aagacggagc agaaatttta attcctatga
     5941 ttgatcattt cttggtaaaa gttgatcgag aaaataaaaa aatcattatg gatttgccag
     6001 aaggtttgat tgaaatgtac ctttaattta gtggtcagtg ttcagttatt agtattcagt
     6061 tgctctaaat ctacaatcaa aaatcgttaa tctacaatca taaatcacct tgtttcaatt
     6121 taagcaattt tcagtcaaac aagaccgaac ggcaatgaaa attggaaccg atggtgtttt
     6181 gcttggcgct tggactcctc tggaaaacaa cccgttcagc attttagaca ttggaacggg
     6241 aacaggaatt atcgctttga tgttagcgca aagaagtgct gccgaacaaa tcgatgcttt
     6301 ggaaattgac gaagacgctt acgaacaagc aacagataat tttgaaaact ccccttggaa
     6361 cgaccgtttg ttttgttttc acgctggctt ggacgaattt gtcgaagaac ccgaagatga
     6421 gtacgattta attgtttcca atccaccttt ctattccgaa gattacaaat ccagtaacga
     6481 ccaacgagat ttggcgcgtt ttcaagatgc aatgcctttt gaagatttga tagaagcagc
     6541 cgctttatta ctttcagaaa acggaatctt ctccgtaatt attcctttca aagaagaaga
     6601 aaaatttttg gctcttgcca atgaatacga attattccct ctaaaaatta cccgcataaa
     6661 aggaactcca acctccgaaa ctaaacgtag tcttttggct tttggccgaa atgaaactcc
     6721 aaattttcct attgatgaat taatcatcga aaccgcacga catatttaca ctccagaata
     6781 tattgccttg acgaaggatt tttatttgaa gatgtaatgt ttatttttta gataatttga
     6841 ttttaattag tagataattt cttaaatttg atatcaaatt ttgacaaaca gacagttaca
     6901 caaaagggca aaatagcgag taatttttta ttaatagatg ggaaaaattg atataaattt
     6961 tgattttcgg ttagacagca agtgtggcga tccagataca gacagtcaaa aattatatga
     7021 tgctcacaaa ttactatgga gtaaagaatt gcctaatgga aaaatgttta attgtgaaat
     7081 caaaggcgat agttatggca gattgcttat aaaaaacaat ttgtatatga acttgtcaag
     7141 cgatagaatg tgtccgcatt ttgacggaaa atacaagaat aaatttgaag gttggctctc
     7201 tgagatagaa attggagaat tgaaacataa agttcgtaca attggaggac atataatttt
     7261 tccagctcat aaaaaaaatg gttttaccgt taatcaagcc agaggtgtaa gtagaataat
     7321 ttgtgatagg tttgatttaa ccttagagtg tataagacgt ttttataaag atgaacaaag
     7381 cccactttat aaaacattaa ttaactataa agattttttt gattcattta ttgattttaa
     7441 aggttatgtt gatttctttt tactacaaga ttttattaac aatcaagagc aaatagagtt
     7501 ttctttacct tttgacaatt ttacgaggcc tccattacct cagactatag acgaatacaa
     7561 acaatataaa acgcatataa tagatttggt aaatagaaga aacaaaagaa ttttagaaaa
     7621 ttatatctaa aaccatcact aaccctaacc catcatcaag actatgaaaa caaatttctc
     7681 cctctttttg accttgtttt taacactagg attgttggct caatcaccag taatttcaaa
     7741 taagaatttc tcttttggta cctatggccg ggctgggatt ggttatggac taggaacgac
     7801 agccgaattt ccgcaatcgc tcaatttgaa cggaatgggt tctattggag gtcgattcga
     7861 agaaaatgac tacttagaat tggctacagc aatgcatttc agccctacaa atgctaataa
     7921 agacatcacc aaaataaatg ttcaggcgcg atttgcattc tacaccaccc aaggacaatt
     7981 aattggcaat gtatccaaca aatctattgg aggaatcacg accgctatgc ccgaattatt
     8041 tgcagaagcc aaaaacctta tgggcagtga ttggagtgtc tggataggag ctagactatt
     8101 ccgtggcgac gatattcata ttattgacca ttattatttt gatgatcatt ctggtcaagg
     8161 cgtgggaata caacacaaaa acaccaagtt ttcggttatt tttacgggct ctatcgacac
     8221 tacttcgacc ttacctccta atttttattt gaacatcgtt aacggaacgc ctactttggg
     8281 attgcgtaat aggtatattt cgattttaga acacgaaatc caagttaaaa agggttatgt
     8341 aaaattaatg ggagaatttc aacgtcttcc ggcaggtgaa gcaaaagatg ccaatacaaa
     8401 atacaactat cctgccgatt atggatttgt tcttggcgcg aaatacaaca aaaacatttc
     8461 gaccaaacat ccaggttcat tcaatgccgt aagcgcccgt tatggaacag gaattgccaa
     8521 tggaggagat ggaggaagca gccgaactta tatgacttat ggtgcgccaa acctcgaaac
     8581 taataattca agaaggcgta ctcaatcgcc cttaccgaaa ctttcttatt gaatctcaac
     8641 gaaaaatatt cactcaacgg ctatgctgtt tataccaaaa gtaaaggggc gagtgacagc
     8701 aaagacaaag cacctgatta tctaggaaaa caaatgttgt tcaaccggaa acaagatatt
     8761 tcttttggag caagaggaac gtattatatt aataattggt tgcatttatt gcacgaactc
     8821 gattttgctt ggcgaaaaga cggcacccaa gattttgcac aaatgaccaa atttactatt
     8881 gcacctacat tagttccaac tgcaaaacga gatgtttggg aacgacctca ttttagattc
     8941 gtatatagtg tagcacatta caacacattc gccgcagaaa acctttactc tccttatctt
     9001 gcccaaagcg gcacaaaaaa ttggggacaa tatgtaggta ttaaaatgga atggtggatt
     9061 tggtaaaaat tttacaatat ttaaaatata acttttactc attgttttgt tatatttctt
     9121 tgtgtagatt tgtgaaataa acttctccaa ctatgaaacc agacttattc caagcgccag
     9181 attattacaa tgttgacgaa ttattgacgg atgaacataa attagttcga gattcggcac
     9241 gcgaatgggt caaacgcgag gtttctccaa taatcgaaga ctttgcacaa agagctgaat
     9301 ttccaaaaca aatcatcaaa ggtttagccg aaattggtgc ttttggacct tatattcccg
     9361 aagaatatgg aggagctgga ttggatcaaa tttcatacgg tttaattatg caggaaattg
     9421 aaaggggtga ttctggcgtg cgttccacgg cttcggttca atcgtcttta gtaatgtatc
     9481 ccatttggaa atacggaaac gaggaacaac gaatgaaata tttacccaaa ctggcttcgg
     9541 gagaaatgat gggttgcttt ggcttgaccg aacccaatta cggttccgac ccaggaagca
     9601 tgattactaa tttcaaagat atgggcgacc attatttatt gaatggtgcc aaaatgtgga
     9661 tttcgaatgc gccctttgcc gacatctcca ttgtttgggc aaaaaatgaa gaaggtagaa
     9721 ttcacggact gattgtagaa cgtggaatgc aaggattttc gacaccggaa acacacaaca
     9781 aatggtcgct tcgtgcctcg gcaactggag aattaatttt tgataatgta aaagtgccaa
     9841 aagagaattt actacccaat aaatcaggat tgggtgcgcc gttgggctgt ttggattctg
     9901 ctcgttttgg aattgcttgg ggagcgattg gagcagcgat ggactgttac gatacg
//
LOCUS       DAOSNN010000074         9922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6796_length_9922_cov_4.778757, whole genome shotgun sequence.
ACCESSION   DAOSNN010000074 DAOSNN010000000
VERSION     DAOSNN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6796_length_9922_cov_4.778757"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLQ70_05345"
     CDS             <1..737
                     /locus_tag="PLQ70_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34074.1"
                     /translation="MKAMRYSFLMVGVWWILFSQFTYYYLPKGNNNSQKVSKHVIFNG
                     FKELKKVWELLGDNIPLKRYLGSFFVSSMAVQTVMLVATYFGAQEIKWESKSDSTTGL
                     IICILIIQLVAVMGAILTSKASEKFGNIPTLIAINSFWVLLCAGAYFIVLPLHFYIMA
                     GLVGLVMGGIQALSRSTYSKLLPETEDTASFFSFFDVAEKIGIVIGMCVYGIIDQITG
                     SPRFAIVFLGIFFLTAVILLKRVPKK"
     gene            826..1569
                     /locus_tag="PLQ70_05350"
     CDS             826..1569
                     /locus_tag="PLQ70_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34075.1"
                     /translation="MKKFRISFSVIILIIATFSLSSCEIEPVDVSSTTPTVPTTPGGG
                     TSSGDYWPTAVNNQWVFKMDGVLQSPMKMISTNTINANTYYTFNEQVGTGIGTTISGV
                     QRLRKLNGNYYLKMENLSSPAQGSIPGFTMTGYETIILKDYLSVGGTWTDKYSQTTSY
                     TDPNFPVISLDFDILATVMEKNSSITIAGHKYTDVIKIKYIQKVTMVGQTTTVTSYYW
                     YSKNVGPIKMTTETGGQTVVQELDSYIVK"
     gene            1762..4212
                     /gene="lon"
                     /locus_tag="PLQ70_05355"
     CDS             1762..4212
                     /gene="lon"
                     /locus_tag="PLQ70_05355"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQE34076.1"
                     /translation="MSNHKIRTLDNLSLQEFDAETELIPLLTPEDEEEMNNEDLPESL
                     SILPLRNMVLFPGVVIPITAGRDKSIKLINDAYAAGKIIGVVAQKNEDVEDPTKNDIH
                     NIGTVARILRVLKMPDGNVTVILQGKKRFQIESVVSETPYFTANIKDVSEKRPTKKDT
                     EFLAILDSVRELAIQIIKENPNIPSEATFAIKNIESQSFLINFVTSNMSLSVAEKQEL
                     LSISSLKERALETLRYMNLELQKLELKNDIQSKVRFDLDQQQREYFLHQQMKTIQEEL
                     GGVSQEEEMDEMSAKAKTKKWDEKTQKHFEKELSKMRRMNPQAPDFGIQRNYLELFLD
                     LPWGEYSKDNFDLKRAQKILDRDHFGLEDVKKRMIEHLAVLKLRNDMKSPIICLTGPP
                     GVGKTSIGRSVAEALGREYVRISLGGLRDEAEIRGHRKTYIGAMPGRIIQSLKKAGTS
                     NPVFVLDEIDKLSNSHNGDPSSALLEVLDPEQNNAFYDNFLEMGYDLSKVMFIATSNN
                     MAEIQPALRDRMEIIKMSGYTIEEKVEIARQHLFPRQLKEHGLTTKDLAIGTKQLEKI
                     VEGYTRESGVRGLEAKIAQVIRNAAKSIAMEEDYNKKVTNEDIVEVLGIPRLERDKYE
                     SNDVAGVVTGLAWTSVGGDILFIESLISQGKGAMTITGNLGNVMKESATIALEYIKAN
                     AELLGLSPEVLTKFNIHLHVPEGATPKDGPSAGIAMLTSLVSVLTQKKVKKGLAMTGE
                     ITLRGKVLPVGGIKEKILAAKRANIKEIILCHENKSDIDEIKPKYIEGLTFHYVKEMS
                     EVLKIAITDQKVKNAKTV"
     gene            4375..5394
                     /gene="porQ"
                     /locus_tag="PLQ70_05360"
     CDS             4375..5394
                     /gene="porQ"
                     /locus_tag="PLQ70_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HQE34077.1"
                     /translation="MPKKLLILFLFFICTVSNGQIGGKYTYQFLNLVTSPRQAAMGGK
                     TITIYDEDVNQAQYNPATINPDMDNHLALNYGSYFGEVTYGTASYAYTYDRHLQTFQV
                     GINYVNYGKFDGYDENGQETSQFTGSEVALSFGYAYNVPNSNIFLGVNGKLISSTLES
                     YNSIGGAVDLGAVLIDEANDVNWALVIRNIGTQFTTYNGINEKLPLDIMIGVSQELEN
                     VPIRWHLTLDNLQQWNISFTNPNQAQTSLDGSTTQKKTSFIGNALRHTTFGAELFPKR
                     GFNLRLGYNFRRAEELKVQDIRNFSGISVGFGLKINKIKFNYSYSRYTLAGNTSLFGL
                     TINLQ"
     gene            5401..6081
                     /gene="cmk"
                     /locus_tag="PLQ70_05365"
     CDS             5401..6081
                     /gene="cmk"
                     /locus_tag="PLQ70_05365"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQE34078.1"
                     /translation="MNKKITIAIDGFSSTGKSTLAKELANYLGYVYVDTGAMYRAVAL
                     FAMQNGYISPNFFDSDALIKRLPEIKLVFKFNADLGFAEMYLNGINVEKEIRTIEVSN
                     YVSKVAEISEVRTKLVEQQQEMGRNKGIVMDGRDIGTVVFPDAELKIFMTASPRTRAE
                     RRFKELMKKGDDVTFEEVLKNVEERDYIDTHRDDSPLVKADDAIEFDNSTISKQEQFE
                     KVLKFIDL"
     gene            6417..6923
                     /locus_tag="PLQ70_05370"
     CDS             6417..6923
                     /locus_tag="PLQ70_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HQE34079.1"
                     /translation="MKTKLLRLALLVAILFTMNSCSSDSSETNSTETQTSTQKAMNYT
                     YNSTELETMALINEYRVSIGLNELKQIEHISYKSEEHDNYMITNNVVNHDDFTSRSED
                     IMKTLKAKTVAENIAFNYNSPKAAVDAWLKSPSHKVNIEGNFTHFGLAIRVNADGKKY
                     YTNIFAKI"
     gene            complement(7338..8144)
                     /locus_tag="PLQ70_05375"
     CDS             complement(7338..8144)
                     /locus_tag="PLQ70_05375"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE34080.1"
                     /translation="MSYLEIKDLEISFPTPKGKYIAVKDINLSIKKGEIISIIGHSGC
                     GKSTIMNAIAGMLTPTGGTVQLDGKTIKGPGPDRGIVFQNYSLLPWLTVHENIFQAVD
                     SVMDCSNKEKHEIVDHYLKMVNLLSHRDKLPGQLSGGMKQRVAIARAFAINPGVLLLD
                     EPFGALDAFTKSSMQLEVLKLWNLNNRDKTIIMITHDIEEALFLSDRIVVLNDGPAST
                     IREIEVVHLPRPRNKIEIVKLPEYIELRDRLLHLLTDKFSIEDMGMVYKS"
     gene            complement(8260..9147)
                     /gene="ntrB"
                     /locus_tag="PLQ70_05380"
     CDS             complement(8260..9147)
                     /gene="ntrB"
                     /locus_tag="PLQ70_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HQE34081.1"
                     /translation="MSDKNILTLDNITTEINTSEAVQKSFLDENSKIKLALNTLIVKS
                     KSVLFAGVGLFLFGGFWSLLSIYTKDALPGPIATLTVLKEMLSDPFYDYGPNDKGIGL
                     QLFNSIKTVLLGFTLGSLIAIPIGILMGASTICKQIFYPIVQLLKPVSPLAWFPIGLV
                     VFKDTGMATIFIVFITSLWSTLINTSFGVASIPQDHKNVAKAFGFSKMRYLTKILIPY
                     SLPHIITGLRLSISVAWLVIVAGEMLSGGAGIGFFVWDSWNALSLEKVISAILIIGIV
                     GLLFDRIFTYIENKVAYKA"
     gene            complement(9243..>9922)
                     /locus_tag="PLQ70_05385"
     CDS             complement(9243..>9922)
                     /locus_tag="PLQ70_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQE34082.1"
                     /translation="VKIITIPPPQMVANMKVGNMDGYCVGEPWGGVAVKQGIGFTQVA
                     SQDIWKDHPEKALVVNKDFSAKRKADLVKVMKAVMEACKWLDVPANRKKAAAIIGKAP
                     YVNAPAEVIENRLMGNYDLGCNQGTEVYDKDYMLFYKGGTVNYPRKSHAIWAMAQFVR
                     FGYLKEAPNYNAIADKLILQDLYEEVAKSMNVKIPTDDMKPFSLTMDKTVFDPSNPAA
                     YLKVVRK"
BASE COUNT         3372 a         1507 c         1768 g         3275 t
ORIGIN      
        1 cgatgaaggc aatgcgttat tcttttttga tggttggagt ttggtggatt ttgtttagtc
       61 aatttaccta ttattatctt ccaaaaggaa ataataacag tcaaaaagta tccaaacacg
      121 tcatttttaa tggtttcaaa gaacttaaga aagtttggga attattagga gataacattc
      181 cattaaagcg ttatttagga agtttttttg tttcaagtat ggcggttcaa actgttatgc
      241 tcgtggctac ttattttgga gcacaagaaa ttaaatggga atccaaaagt gatagtacaa
      301 caggtttgat tatttgtatt ttgatcattc aattggtggc ggttatggga gctattttaa
      361 cttctaaagc ttctgagaaa tttggaaata tcccaacgct aattgctatt aatagttttt
      421 gggtattgct ttgtgctgga gcttatttta tcgttttgcc acttcatttt tatataatgg
      481 cgggtttagt gggtctcgta atgggcggaa ttcaagcttt atcccgttcg acttattcta
      541 aattattgcc cgaaacagaa gatacagctt ccttttttag tttttttgat gtagctgaaa
      601 aaataggtat tgtaatcgga atgtgtgttt atggaattat tgatcaaatt acaggaagtc
      661 ctagatttgc aattgtattt ttaggtattt ttttcctaac tgcggtaatt ttgttaaaaa
      721 gagttcctaa aaaatagttg tcttattaga aaaaaatcgt gttaaaatta taataaaaaa
      781 attatatttg tataaaaatt gccacaatca atagaaccta caaatatgaa aaaatttaga
      841 atctcatttt cggtaattat tctaataatt gccacatttt ctttaagttc ttgtgaaata
      901 gaacccgtag atgtttcatc aacaactcca acagtaccaa cgactccagg tggaggaact
      961 tcttcgggag attattggcc aaccgcagta aataatcaat gggttttcaa gatggatggc
     1021 gtattgcaaa gcccaatgaa aatgatttcc acaaatacaa taaacgcaaa tacatattat
     1081 acttttaatg aacaagtagg aacaggaata ggtaccacta tttctggagt gcagcgatta
     1141 agaaaattga acgggaatta ttatttaaag atggagaatt taagttcacc tgcacaagga
     1201 tcaataccag gttttacaat gactggatat gagactatta tattaaaaga ttatttatct
     1261 gtgggaggaa cttggactga taaatattct caaacaacta gttatacaga tccaaatttc
     1321 ccagttatct ctttagattt tgatatttta gcaactgtta tggagaagaa tagttccatt
     1381 actattgcgg gtcataaata tactgatgta atcaaaatta aatatattca gaaggtaact
     1441 atggttggtc aaactacgac agttactagc tattattggt attccaaaaa tgtaggccca
     1501 attaaaatga ctacagaaac cggaggtcaa acagttgttc aagagttaga ttcatacatt
     1561 gtgaaataag tgaaaatttt aagcaagaaa aaacctgaac tttgttcagg ttttttttat
     1621 ggcacaaaga ttgaattata ttgttatata aattagatta tatttgttgt tgattgattt
     1681 tcagcaccgt atattaaaat aatattaact ttgtttttaa atgatttcct tatttaatga
     1741 caaaaagact taaaaactat tatgtcaaat cataaaatac gcacacttga caatctgtca
     1801 cttcaagaat ttgatgccga aactgaatta attcccttat tgactcccga agacgaggag
     1861 gaaatgaaca acgaagattt acccgaaagt ttatctattt tgcctttgcg aaatatggtt
     1921 ttatttcctg gagttgtcat tccaattacg gcaggtcgtg ataaatcgat aaaattaatc
     1981 aatgatgctt atgcagcagg aaaaatcatt ggtgttgttg cccaaaaaaa tgaagatgtc
     2041 gaagatccta ctaaaaatga tattcataat ataggaactg ttgctcgaat tttgagagtt
     2101 ctaaaaatgc ctgatggcaa tgtaactgtt attcttcaag gaaaaaaacg ttttcaaata
     2161 gaatcggttg tttctgaaac tccttatttt acagcaaata ttaaggatgt ttctgaaaaa
     2221 agacctacta aaaaagacac tgaattttta gctattctgg actcggttag agaattggca
     2281 attcaaatta tcaaagaaaa tccgaatatt ccaagcgaag ctacatttgc tattaaaaac
     2341 attgaaagcc aatcgttttt aatcaatttt gtgacttcaa atatgagttt gtcagttgca
     2401 gaaaagcaag agttgttgtc tattagttct ttaaaagaaa gagctttaga gactttgcgt
     2461 tatatgaatt tggagttgca aaaactcgaa ttaaaaaacg atattcaatc taaagttcgt
     2521 tttgatttag accaacaaca acgcgaatat ttccttcatc agcaaatgaa aaccattcag
     2581 gaagaattgg gtggcgtttc gcaagaagag gaaatggacg aaatgagtgc caaagccaaa
     2641 accaaaaaat gggacgaaaa aactcaaaaa catttcgaaa aagaattgtc taaaatgcgt
     2701 cgaatgaatc cgcaagcacc tgattttggt attcaacgaa attatttaga attgttttta
     2761 gacctgcctt ggggcgaata ttctaaagat aattttgatt tgaaacgtgc ccaaaaaatt
     2821 cttgaccgag atcattttgg attggaagat gtcaagaaac gaatgatcga acatttggcc
     2881 gttttgaaat tgcgaaacga tatgaaatca cctattattt gtttgacagg ccctccgggt
     2941 gttggtaaaa cctctatcgg acgctcggtt gccgaagctt tgggaagaga atatgttcga
     3001 atttcattgg gaggtttgcg tgacgaagct gaaattcgtg gtcatcgtaa aacctacatt
     3061 ggcgcgatgc caggaagaat tattcaaagt ttgaaaaaag caggaacctc taatccggtt
     3121 tttgttttag acgaaataga taaattatcc aatagccata acggcgatcc atcttcggct
     3181 ttgttagaag ttttagatcc agaacaaaac aatgctttct atgataattt ccttgaaatg
     3241 ggttatgatt tatccaaagt gatgttcatt gctacgtcta acaatatggc tgagattcaa
     3301 cccgctttga gagatcgtat ggaaattata aaaatgtcgg gttacactat tgaagaaaaa
     3361 gtagaaattg ctcgtcagca tttgtttcca agacaattga aagaacacgg attaacgacc
     3421 aaagatttag caattggcac aaaacaatta gaaaaaattg tcgaaggtta cacacgtgaa
     3481 tcgggtgttc gtggtttgga agcaaaaatt gctcaggtga tccgtaatgc ggcaaaatct
     3541 attgcaatgg aggaagatta caacaagaaa gtaactaacg aagatattgt tgaagttctt
     3601 ggaattccta gattagagcg cgataaatat gaaagtaatg atgtagcagg agttgtaaca
     3661 ggactggctt ggactagtgt tggaggcgat attcttttta tagaatcttt gatttcgcaa
     3721 ggaaaaggag cgatgaccat taccggaaat ttgggtaatg tgatgaaaga atcggcaaca
     3781 attgccttag aatacatcaa agccaatgca gaattacttg gattaagtcc tgaggtgttg
     3841 accaaattta atattcattt acacgttccc gaaggagcta ctccaaaaga cgggccaagt
     3901 gcaggtatag ctatgttgac ttcattagtt tcggtcttga ctcaaaagaa agtgaagaaa
     3961 ggattggcga tgactggtga aattactttg cgtggaaaag ttttgcctgt gggcggaata
     4021 aaagaaaaga ttttggctgc caaaagagcc aacatcaaag aaattatctt gtgtcacgaa
     4081 aacaaaagcg atatcgacga aatcaaacct aaatacattg aaggtttgac ttttcattat
     4141 gtgaaagaaa tgagcgaagt attgaaaatt gccattaccg atcaaaaagt aaaaaacgca
     4201 aaaacagtat aattgctttt ttttgatacc aatagaattc catctcgaag cttcgggatg
     4261 gaattttttt tcaaatagaa attgcttgta aacttcgatt aaaaaaaagg aatttattct
     4321 attataattt tatcttcgca tttgcgtttt acaataccaa tagcgatttc aaatatgcca
     4381 aaaaaacttt taattctctt tttatttttt atttgtactg tttcaaacgg tcaaatagga
     4441 gggaaatata cctatcagtt tttgaacttg gttacttcgc caagacaagc cgcaatggga
     4501 ggtaaaacga ttaccattta tgacgaagac gtcaatcagg cacaatataa tccggctaca
     4561 ataaatcctg atatggacaa tcatctggca ctgaattatg gtagttattt tggcgaagtt
     4621 acctacggaa ctgcttcgta tgcttatact tacgaccgcc atttgcaaac ttttcaggtg
     4681 ggaatcaact atgtaaatta cggtaaattt gatggatatg acgaaaatgg tcaggagact
     4741 tcacaattta cgggtagtga ggtggcattg tcttttggat atgcttataa tgtacctaat
     4801 agtaatattt ttttaggagt caatggaaaa ctgatttctt cgactttaga aagttacaat
     4861 tctattggtg gagctgtcga tttgggagcg gttttaattg atgaagccaa cgatgtaaac
     4921 tgggcgttgg tgattcgaaa tattggaaca caatttacaa cttataatgg cattaacgaa
     4981 aaattgcctt tggatataat gattggagtt tcgcaagaat tagagaatgt acctataaga
     5041 tggcatttga ctttggataa tttgcagcaa tggaatattt cttttacgaa tcctaatcag
     5101 gcacaaactt cactcgatgg aagtacaact cagaagaaaa cgtcttttat aggaaacgca
     5161 ttaagacata ccacttttgg tgcagaactt tttccaaaaa gaggatttaa tttaagattg
     5221 ggctacaatt ttagacgagc agaagaactg aaggtacaag atattagaaa tttctctgga
     5281 atatctgttg gttttggatt aaaaattaac aaaataaaat tcaattattc gtattctcga
     5341 tataccttag caggaaatac gagtttgttt gggttgacta taaatttaca ataatacatt
     5401 ttgaataaaa aaattaccat agcaatagat ggattttcat caaccggaaa aagtactttg
     5461 gcaaaagaat tggcaaatta tttaggatat gtttatgtcg atacaggtgc tatgtatcgt
     5521 gctgttgctt tattcgcgat gcaaaacggc tatatttcac ctaatttttt tgattcggat
     5581 gcgctgatta aaagattgcc agaaattaaa ttagttttca aatttaatgc tgatttgggc
     5641 tttgccgaaa tgtatttgaa tggaattaat gtcgaaaaag aaattcgtac tattgaagtt
     5701 tcaaattatg ttagtaaagt tgccgaaatt tcggaagttc gtacaaaact agttgaacaa
     5761 cagcaagaaa tgggtagaaa taaaggcatc gtgatggatg gaagagacat aggaacggta
     5821 gtttttccag atgctgaact aaaaatattt atgactgcca gtccaagaac acgtgccgaa
     5881 agaagattca aagaattgat gaagaaaggt gatgatgtaa cctttgagga agttttaaaa
     5941 aacgttgagg aaagagatta tatagatacc catcgtgacg attctccatt ggttaaggca
     6001 gatgatgcca ttgaatttga caattctacg atttcgaaac aagaacaatt tgaaaaagtt
     6061 ttgaaattta ttgatttata agactatact atcatttttt tatgatttaa gcctcgctat
     6121 ttgcggggct tttttatagg ttgttttgtg attttataaa ctatatattt tatacaaaat
     6181 attgacaaaa ataaagtcat tcaaatgatt tagttcatct attagaaaat cttcgtaata
     6241 ttcgattatt ttgtaagata aaatacttta aatacgtaaa tgttgtgtat ttcatcgatt
     6301 atatttgttt ttaaacgata ttgagcgtaa atttactcaa gaattaaaaa ggaagaatat
     6361 tataccttaa agaagtttgc cccacaatct actttaaatt taatctactc attattatga
     6421 aaacaaaatt acttcgttta gcattgctag tagcaattct attcacaatg aattcttgtt
     6481 catcagatag ttctgagaca aatagtactg aaactcagac ttcaactcaa aaagcaatga
     6541 attatactta taattctacc gagttagaaa caatggcttt gattaatgaa tatcgagtta
     6601 gtattggttt aaatgaattg aaacaaattg aacatatttc atataaatca gaagaacacg
     6661 ataattatat gataactaat aatgtagtga atcacgatga ttttacttct cgttcagaag
     6721 atattatgaa aacattaaaa gcaaaaacag tagcagaaaa tattgcgttt aattataatt
     6781 ctccaaaagc agctgttgat gcttggttaa aaagccctag tcataaagta aacattgaag
     6841 gtaattttac tcattttggg cttgctatta gagttaatgc tgatggtaaa aaatactaca
     6901 ctaatatttt tgctaaaata taaattctcg atactaatat aattgaaact gtcatgagaa
     6961 tggcagtttt tttttgttta ataaatacta agattagtaa tctattgaac gattttatct
     7021 gtttttgggt atattcatta ttctatttat gggtttcaaa atagttaaaa ctatgcactt
     7081 tagtatattt cgctttcagt ttataaaaaa attcaaccgc agattcgtaa attttaaaaa
     7141 tagttataat tggaataatt tgcgaatcta cggtaaaaat tcataatcca accgtgcaga
     7201 catttagtct gccaaccaag cctatctaat tctaaagttt taccgatttg atttcatgaa
     7261 cacccattcg aattataaaa aaaaacctgt gatgcaaaaa catcacagga attttcaacc
     7321 aactaattca cttaaaacta acttttgtac accattccca tatcttcaat agaaaattta
     7381 tcggtcaata ggtgtagtaa tcgatctctt aattcgatat attcgggtaa ttttacaatt
     7441 tctattttgt ttctaggtct tggcaaatgc acgacttcaa tttctctaat tgtcgaagct
     7501 ggcccatcgt ttaaaaccac aattctgtcc gacaagaata atgcttcttc gatatcgtga
     7561 gtaatcataa taatggtttt gtcacgatta ttaagattcc ataattttaa tacttctaat
     7621 tgcatcgaac ttttggtgaa ggcatccaat gcgccaaaag gttcgtctaa taataatact
     7681 ccaggattga tagcaaaggc tcttgcgata gcaacacgtt gtttcattcc gccagataat
     7741 tgacctggta atttatctct gtgggacaat aaattcacca tttttagata atgatcaaca
     7801 atttcatgtt tttccttatt agaacaatcc attaccgaat ctacggcttg aaaaatattt
     7861 tcgtgtactg ttaaccaagg taaaagagag tagttttgaa acacaattcc tctatcaggg
     7921 cctggacctt ttatagtttt tccgtctaac tgaacagttc ctcctgttgg tgttagcatt
     7981 ccagcaatgg cattcataat ggttgattta ccacatccag agtgaccaat tatagagatt
     8041 atttctcctt tttttataga taaattaata tcttttacgg caatatattt tccttttgga
     8101 gttggaaaag agatttccaa gtctttgatt tctaaatagc tcattttgat ttgtttttag
     8161 tgggtttgat atgatttggt ttaatattta aagttgttta agttcttttc gggtttaaac
     8221 aaccataaac tcttaaactc ttaaactttt aaactttttt tacgctttat aagctacttt
     8281 gttttcgata taagtgaata ttcggtcaaa aagcaaacct acgattccaa tgataaggat
     8341 ggctgagatc actttttcta gactaagtgc attccaactg tcccaaacga agaacccaat
     8401 tccagctcct cctgacaaca tttcgccggc aactataacc aaccaagcca cactaatact
     8461 tagacgtaaa cctgtaataa tgtgaggtaa actataagga attaatattt tagttaaata
     8521 tctcatttta gaaaaaccaa aagctttggc cacattttta tggtcttgag gaatcgatgc
     8581 tacaccaaaa gaagtattaa taagagtgga ccataatgaa gtaataaaaa ctataaagat
     8641 agttgccatt ccagtatctt tgaaaactac taaaccaatt ggaaaccaag ccaaaggaga
     8701 aacgggttta agtaactgca caattggata aaaaatttgt ttgcaaatag tgctggcacc
     8761 catcaaaatt ccgattggaa tggcaattaa tgaacctaaa gtaaatccta aaagaacggt
     8821 ttttatagag ttaaaaagtt gtaaaccaat tcctttgtcg tttggaccat aatcataaaa
     8881 tggatcgctc aacatttctt tcaaaacggt tagtgtggca attggtcctg gtaaagcatc
     8941 tttggtataa atactcaata agctccaaaa acctccaaaa aggaataaac ctacgccagc
     9001 aaaaaggact gattttgatt ttacaattag ggtatttaaa gcaagtttga tttttgaatt
     9061 ttcgtctaga aaactttttt gaacagcctc agaagtattg atttctgtag tgatgttgtc
     9121 taaggttaaa atgtttttat ctgacatggt actctagttt tagttttaat atgaaaaatg
     9181 attctggttg atgacattct ttttccctcc caaaaggagg gaatagaatg tagttttatt
     9241 ttttattttc ttacaacttt aagataagct gctggatttg acgggtcgaa aacggttttg
     9301 tccatagtaa gagagaacgg tttcatatcg tcagtaggaa tttttacgtt catacttttg
     9361 gctacttctt catacaaatc ttgtagaatt aatttatcgg caattgcatt ataatttgga
     9421 gcttctttca aatatccgaa tctaacgaat tgtgccatag cccaaattgc gtgagattta
     9481 cgtgggtaat ttactgttcc acctttgtaa aacaacatat aatctttgtc atacacttct
     9541 gtaccttggt tgcaacctaa atcataattc cccatcaatc tgttttcgat tacttctgct
     9601 ggagcattta catatggagc tttaccaatg attgctgctg cttttttacg attggcagga
     9661 acatctaacc atttgcaggc ttccataacg gctttcatta cttttaccaa atcagctttg
     9721 cgttttgcgc taaaatcttt gttgactacc aatgcttttt ctgggtgatc tttccaaata
     9781 tcttgtgaag caacttgagt aaatccaata ccttgtttta ctgcaacacc tccccaaggc
     9841 tctccaacac aatagccatc catattacct actttcatgt tggctaccat ttgtggaggg
     9901 ggaatagtaa tgatttttac ag
//
LOCUS       DAOSNN010000075         9892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6821_length_9892_cov_4.265731, whole genome shotgun sequence.
ACCESSION   DAOSNN010000075 DAOSNN010000000
VERSION     DAOSNN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6821_length_9892_cov_4.265731"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /gene="ilvN"
                     /locus_tag="PLQ70_05390"
     CDS             complement(<1..506)
                     /gene="ilvN"
                     /locus_tag="PLQ70_05390"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQE34083.1"
                     /translation="MENKIFTISVYSENNVGLLNRISGIFLKRHINILSLNVSESEIE
                     NVSRFIIVVNTTEKWVHNIVGQIEKQIEVIKAFYHIDEETIFLESAIFKIESSLLFDE
                     RQIQNIIKESHSEIVTVSRDFFVISKSGKRSEINDLYNKLKPYGIMQFVRSGRISVSK
                     EKMEISTI"
     gene            complement(602..2299)
                     /gene="ilvB"
                     /locus_tag="PLQ70_05395"
     CDS             complement(602..2299)
                     /gene="ilvB"
                     /locus_tag="PLQ70_05395"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HQE34084.1"
                     /translation="MENNKISGAEAVIRCLLAEGVDLVYGYPGGAIMPVYDELYKFQD
                     QLHHVLVRHEQGAAHAAQGYARVTGKVGVAIATSGPGATNLVTGIADAQIDSTPLVCI
                     TGQVGKHLLGSDAFQETDIIGISTPVTKWNYQVTEAHEIPEIIAKAFYIAKSGRPGPV
                     LIDITKNAQFDTLDFDYKKCTGIRSYLPKPILNLQKVQEAADLINNAKKPFIVFGQGI
                     ILSQAEEQLKRFVEKSGIPAAWTILGLSALPTEHPLNVGMVGMHGNYGPNILTNECDV
                     LIALGMRFDDRVTGNLATYAKQAKIIHFEIDPSEVDKNVKTDIAVLADVKESLTAIIP
                     LIDKKSHDSWHNEFKEKYKIEFDAVINEELIPTNGKGISMGETIEMINKHSKGDAIMV
                     SDVGQHQMFACRYAKFNSSKSNVTSGGLGTMGFALPAAIGAKMGQPNREVVAIIGDGG
                     FQMNIQELGTIFQTQVPVKIVVLNNEFLGMVRQWQELFFDNRYASTKMINPNFVAIAE
                     GYHIKSKRISQREDLDDAVAEMLASKDSYFLEVMVEKENNVFPMIPTGASVSDMRLS"
     gene            complement(2388..3563)
                     /locus_tag="PLQ70_05400"
     CDS             complement(2388..3563)
                     /locus_tag="PLQ70_05400"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQE34085.1"
                     /translation="MNREKVQIFDTTLRDGEQVPGCKLDTAQKLVIANRLDEMGVDII
                     EAGFPVSSPGDFLSVSEISKIVKNATVCGLTRAVKNDIDVAGAALKYAKKPRIHTGIG
                     TSNSHIVHKLNTTREDIIARAKYAVSYAKSYVEDVEFYAEDAGRTENDFLARVCEEVI
                     KSGATVLNIPDTTGYCLPEEYGAKIKYLKENVKGIENVIISCHCHNDLGMATANSIAG
                     VINGARQIECTINGIGERAGNTALEEVVMILKQHPYLNLDTSINSRQLNEMSRLVSDS
                     MGMIVQPNKAIVGANAFSHSSGIHQDGVIKNRETYEIMDPLEVGVTESSIILTARSGR
                     AALAYRAKKVGYELTKVQLDIVYVEFLKFADLKKEVLDSDIHQIVEACKIEGDIIRC"
     gene            3718..5262
                     /locus_tag="PLQ70_05405"
     CDS             3718..5262
                     /locus_tag="PLQ70_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34086.1"
                     /translation="MANHQKDSLFVLIKSLSKSEKRQFKIFASRLETSSNTKFIELFN
                     ILDKSEAYDEKLILKSGIIKKVQLSNLKSYLYKQILVSIRLNIPSQNIRYQLREQIDF
                     AAILYNKGLYKQSLKILDKTKQQALENDEKYMAYEIVEFEKLIESQYITRSIQGRADE
                     LVIQAKELNYKNTISSKLSNLSLQLYGIMLKTGYVRSDEEYKYIDDYFKKHISKFDEK
                     KYGFRERYWYFNANLWRSFLVQDFLSCYKFSHKWVTLFYDNRNMIFLNPVFFLKGNHY
                     LLESLFMLKYKTNFKKYLEMLEETINDPQFPVNDNITSLSFLYLYNNKLNYHILEGTF
                     AEAEYLIPEVLDKIDLHNEHLDEHHEMLFYYKIACIYFGNEKYNECVFYLEKIINNKN
                     LYVREDLACFARLLCLIAHYESGKDFNLENQLVSTYKFLIKMNDLHEVQKEIIRFLKK
                     LSTIYPSDIKKEFIKVKERFVELEKNTYEKRAFLYLDIISWLESKIENRKISDIIKEK
                     AKLSNR"
     gene            complement(5280..5978)
                     /locus_tag="PLQ70_05410"
     CDS             complement(5280..5978)
                     /locus_tag="PLQ70_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1826 domain-containing protein"
                     /protein_id="HQE34087.1"
                     /translation="MSNTFSDNRQIGVVYTFSELVNTHFKEEMNALCWYRNLEGDFKE
                     IVGQLQLKENITEVDPEDLLSLQLSKKGKLAREIILNDWRLLSDFGASPSLNLLKCYE
                     RDDEFDFISTDVYSYHVDRSPIATDTFLCTYHGAASDIIANEQAEQKILIPEIRKKLA
                     VLYDGPSDEFETFLKENFFDLHYQAHPNAAPINLGLGHLWRLAVDHPKQEVLPCIHRA
                     PIENEGEYRLLLIC"
     gene            complement(6203..8179)
                     /locus_tag="PLQ70_05415"
     CDS             complement(6203..8179)
                     /locus_tag="PLQ70_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HQE34088.1"
                     /translation="MTFDRKNLTNEQLLYLYTSLLMPRLIEEKMLILIRQGKVSKWFS
                     GIGQEAIAVGVTAVLDADEYILPMHRNLGVFTGRNIPMHRLFSQWQGKADGFTKGRDR
                     SFHFGTQEFKIIGMISHLGPQLGIADGIALANKLNQNGKVTAVFTGEGATSEGDFHEA
                     LNIAAVWELPVLFIVENNGYGLSTPTNEQFRCENIADKGVGYGIESHIIDGNNILEVY
                     HKLSELKASMVKNPRPVLLEFKTFRMRGHEEASGSKYVPQELIAVWTTKDPVENYRKF
                     LSENEILSDDLDALLHTEIKAAIDENWALANTESEIEATYDDELNDVYKPFQFQEVIP
                     SEETKNIRFIDAVSNALKQSMERHHNLVIMGQDIAEYGGAFKITEGFVEQFGKERVRN
                     TPICESAVVSAAMGLSINGYKAIVEMQFADFVSTGFNPIVNLLAKTHYRWQEKADVVV
                     RMPCGAGTQAGPFHSQTNEAWFTKTPGLKVVYPAFPYDAKGLLNESINDPNPVLFFEH
                     KQLYRSIHQEVPTNYYTIPLGKAALLKEGNQVTIIAYGAAVHWALETLAKKPEISADV
                     LDLRTLQPLDAEAIYASVKKTGRAIILQEDSMFGGIASDISALIMENCFQYLDAPVKR
                     VASLDSPIPFTKALEDQYLPKGRFEKELLELLRY"
     gene            8335..8769
                     /locus_tag="PLQ70_05420"
     CDS             8335..8769
                     /locus_tag="PLQ70_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34089.1"
                     /translation="MKKKLIILSMLLGGFTYSNAQSIGTLTKAATSSATQQATSAAAN
                     SFDVAGISNQVMGVLSPKLKLTDAQKPLVNSLVNETLNKKKSILPTALSDKAGYASKM
                     LGISNGFTSKMKGLIKPEQYTALLGILPKSASSTSALAKMLF"
     gene            complement(8967..9152)
                     /locus_tag="PLQ70_05425"
     CDS             complement(8967..9152)
                     /locus_tag="PLQ70_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQE34090.1"
                     /translation="MKNRIVRAVAGTFILISLLLAIYVNQNWLWFTAFVGANLLQSSL
                     TKWCLMDDILKKLGVKD"
     gene            complement(9173..9481)
                     /locus_tag="PLQ70_05430"
     CDS             complement(9173..9481)
                     /locus_tag="PLQ70_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34091.1"
                     /translation="MKLLLITAVREFEKDIKQILKKVQVKSFSYKDVKGFKDNSEDAL
                     EANWFATDRQETESVLFYAFVKEEKVDGLFEMVNDFNTEQVSKSNIHVAVLNIERCSN
                     "
     gene            complement(9546..>9892)
                     /locus_tag="PLQ70_05435"
     CDS             complement(9546..>9892)
                     /locus_tag="PLQ70_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQE34092.1"
                     /translation="IALAGVMVRNSVLLIDFIEIRLNEGVPLKQAIIEAGAVRTTPIL
                     LTTGAVVIGASIILFDPIFQGLAISLVFGAVVSTVLTLLVVPIIYYITERKKWETSPD
                     PSKGGEIGTPNE"
BASE COUNT         3131 a         1881 c         1606 g         3274 t
ORIGIN      
        1 aatattgtgg atatttccat tttttcttta gaaactgaaa ttcgtcctga acgaacaaat
       61 tgcataattc cataaggttt taatttgttg tataagtcat tgatttcgga gcgttttcct
      121 gattttgata tcacaaaaaa gtctctagat acagtgacta tctccgaatg actttctttt
      181 ataatgtttt gaatttgtct ttcgtcgaat aataagctgg attcaatttt gaaaatagca
      241 ctttcaagaa agatagtttc ttcatcaata tgatagaaag ctttgataac ttctatttgt
      301 ttttcgattt gaccaacaat attatgcacc catttttcgg tagtgtttac cacaataatg
      361 aaacgggaaa cattttcaat ttccgattcg gatacattca aactcaatat attgatgtgg
      421 cgtttcaaga aaattcctga aattcgattc aataagccaa cgttgttttc tgaataaaca
      481 gaaatggtga atattttatt ttccattttt tattttttgt ttaaaggttt aatcgtttaa
      541 aagtttaatc gtttaaaatt gaataacttt aaacccctaa acttttaaac ttttaaactt
      601 tttaggataa cctcatatca gaaaccgaag ctcctgttgg tatcattggg aatacattgt
      661 tttctttttc taccataact tccaagaagt aagaatcttt tgaagccaac atttcggcaa
      721 cggcatcatc caaatcttct ctttggctaa ttcttttcga tttgatgtga tatccttcag
      781 caatggctac aaaattcgga ttgatcattt tggtcgatgc ataacggtta tcgaaaaaca
      841 attcttgcca ttggcgtacc atccctaaaa attcattgtt caaaaccaca attttcacag
      901 gcacttgggt ttgaaaaatt gtacccagtt cttgtatgtt catttgaaaa cctccatcgc
      961 caatgatagc tactacttcg cgattgggtt gtcccatttt ggcaccaatt gcagcaggca
     1021 aagcaaatcc catagttccc aatccgccag aagtaacgtt acttttggat gaattgaatt
     1081 tggcataacg gcaagcaaac atttgatgtt gtcctacgtc cgaaaccatt atagcatctc
     1141 cttttgagtg tttattaatc atttcgatag tttctcccat cgaaattcct ttaccatttg
     1201 ttggaatcaa ttcttcgttg ataacggcat caaattcaat tttatatttt tccttaaatt
     1261 cattgtgcca agaatcgtgc gattttttgt caattaaagg aattatagct gtcaaagatt
     1321 cttttacatc ggctaaaacg gcaatatctg ttttcacgtt tttatccact tcagaagggt
     1381 caatttcgaa atgaattatt ttggcttgtt tggcataagt agccaaattt cctgtaactc
     1441 ggtcgtcaaa acgcattcca agtgcgatta atacatcgca ttcattagtc aatatattgg
     1501 ggccgtaatt accgtgcatt cccaccattc ccacatttaa tgggtgttct gttggcaaag
     1561 ctgaaagtcc caaaatagtc caagcagctg gaattcctga tttttctacg aatcttttta
     1621 attgctcttc ggcttgactt aaaataatac cttgaccaaa aacgatgaat ggttttttgg
     1681 cattattaat caaatcggct gcttcttgta ctttttgaag attcaaaatc ggtttaggaa
     1741 ggtaacttct aattcccgta cattttttat aatcaaaatc taaagtatcg aattgagcat
     1801 ttttggtaat atcaatcaaa acgggacctg gacgacctga tttggcgata taaaaagcct
     1861 tggcaattat ttctggtatt tcgtgagctt cggtaacttg atagttccat ttagttactg
     1921 gcgtcgaaat tccgatgata tccgtttcct gaaatgcatc cgaacccaat aaatgttttc
     1981 cgacttgacc tgtaatacaa accaaaggag tcgaatcgat ttgagcatcg gctattcctg
     2041 taactaaatt cgtggctcct ggtcctgaag tagcaatagc cacccctact tttccagtaa
     2101 ctctagcata accttgggca gcgtgagccg caccttgttc gtgacgcacc aaaacgtggt
     2161 gcaattgatc ttgaaattta tataattcgt cgtaaactgg cattatggct ccacctggat
     2221 atccataaac caagtcaaca ccttcggcta ataagcatct aataaccgct tctgcacctg
     2281 atattttatt attttccatt attattttct ttcctttctc aactttctta taatttagga
     2341 agccgagatt actattttaa tagtattact atttttctgc ttttaaatta gcatctaatg
     2401 atatcgcctt caattttaca ggcttcaaca atttgatgaa tatctgaatc taaaacttct
     2461 tttttaaggt ctgcgaattt caagaattcg acatagacaa tatccaattg cacttttgtc
     2521 aattcataac ccactttttt agctctgtaa gccaatgctg ctctaccact tcttgctgta
     2581 agaatgatag aagattcggt gacacccact tctaaaggat ccataatttc ataggtttcc
     2641 ctatttttta taactccgtc ttggtgaatt ccagaactgt gtgaaaaggc gtttgctcca
     2701 acaattgctt tattgggttg cacaatcatt cccatactat ctgagaccaa tctactcatt
     2761 tcattcaatt gtcgagaatt gatactggtg tctaaattca aataaggatg ttgttttagg
     2821 atcattacca cttcttcaag tgctgtgttt cctgctcttt ctcctattcc attgatagta
     2881 cattctattt gtcttgcacc atttattact cctgcaatag aattggcagt cgccatacct
     2941 aaatcattat gacaatgaca agaaataatt acgttttcaa ttccttttac attttctttt
     3001 aggtatttta tttttgcacc atattcttct ggcaaacaat atcctgttgt gtctggaata
     3061 ttcaatacgg tagctcctga ttttatgact tcctcacaga ctcgtgccaa gaaatcattc
     3121 tctgttcggc ctgcatcttc agcataaaat tctacatctt cgacataaga ttttgcataa
     3181 gaaaccgcat attttgctct agcaataata tcttctcttg tggtatttaa tttgtgtact
     3241 atatgtgaat ttgatgttcc aattccagta tgaatacgtg gtttttttgc atatttcaaa
     3301 gctgctccag caacatctat atcatttttt acagctcttg taagtccgca aacagtggca
     3361 tttttcacaa tcttgctgat ttcagaaacg gataaaaaat cgccgggact tgaaactgga
     3421 aaacctgctt caataatatc aactcccatt tcgtccagtc gattggcaat aactaatttt
     3481 tgcgcagtat ctagtttaca tcctgggact tgttcgccgt ctcttaaagt ggtatcaaaa
     3541 atttggactt tctctctatt cattttaaaa tatttaatgt aatttcacat ctttgaacaa
     3601 aaatacattt cattactcta ttataaaatt cattatttag gaattatact gtttataagg
     3661 aatataaaag cattataact atcttaatta caacatatta cattacttta ttttacaatg
     3721 gctaaccatc aaaaagattc tttatttgtt ttaataaaat cactttctaa gtctgaaaaa
     3781 aggcaattca agatttttgc gagtcgtctt gaaacgagtt cgaatacaaa atttatcgaa
     3841 ttattcaata ttttggacaa atcggaagcc tatgacgaga aacttattct gaaaagcggt
     3901 atcatcaaaa aagtgcaatt atccaatctt aaatcgtatt tatacaaaca aatattggtg
     3961 agcattcgct tgaatattcc tagccaaaac attcgctatc agctaagaga acaaattgat
     4021 tttgctgcca tactttacaa caaaggtttg tacaagcaaa gtttgaaaat cttggataaa
     4081 accaaacaac aagctttaga aaatgacgaa aaatacatgg cttatgaaat tgtcgaattc
     4141 gaaaaactaa ttgaatcaca atatattacg cgcagtattc aaggtcgagc cgatgaatta
     4201 gttattcaag caaaagagct gaattataag aatactattt cgagcaaatt atcgaatttg
     4261 tctttgcaat tgtacggcat tatgctgaaa acaggttatg tacgaagtga cgaagagtac
     4321 aaatacatag atgattattt caaaaaacac atctccaaat ttgacgaaaa aaagtatgga
     4381 ttccgtgagc gatattggta tttcaatgct aatctttggc gaagtttctt agttcaggat
     4441 ttcttgtcgt gttacaagtt ttcgcataaa tgggttacgc ttttttatga caatagaaat
     4501 atgattttcc taaatccagt gtttttcttg aaaggaaatc attatttatt ggaatcgctt
     4561 tttatgctca aatacaagac caatttcaag aaatatctag aaatgttaga agaaacgatc
     4621 aatgatcctc agtttcctgt caatgacaat atcacttcgc tttcattttt gtatttgtac
     4681 aataacaaac tcaattatca catcctcgaa ggcacatttg ctgaagccga atacttgatt
     4741 ccagaagtgt tagacaaaat agatttgcac aatgagcatt tggacgagca tcacgaaatg
     4801 ttgttttact acaaaattgc ttgtatttat tttggcaacg aaaaatacaa tgaatgtgtg
     4861 ttttatttag agaaaatcat caataacaaa aacctttatg ttcgcgaaga tttggcttgc
     4921 tttgcccgat tgttgtgttt gattgcgcat tacgaatctg gaaaagattt caacttagaa
     4981 aatcagttgg tgagcaccta taaattttta atcaaaatga atgatttgca cgaagtccaa
     5041 aaggaaatta ttcgcttttt gaaaaaatta agcaccattt atccaagtga catcaaaaaa
     5101 gaattcatta aggtaaaaga acgttttgtc gaattagaga aaaacaccta cgagaaaaga
     5161 gcgttccttt atctagatat catttcttgg ctagaaagca aaatcgaaaa ccgcaaaatt
     5221 agtgacatta tcaaggaaaa agcgaagttg agtaatcgat aataggtaaa atataacaat
     5281 caacaaatca gcaacaaacg atattctcct tcattttcta tgggtgctct gtgaatacaa
     5341 ggcagaacct cttgtttggg atgatccacg gccagacgcc aaagatgtcc cagtcccaaa
     5401 ttaatgggtg cggcattggg gtgagcctga taatgcaaat caaagaaatt ttcctttaaa
     5461 aaggtttcaa attcatctga tgggccatca tatagtactg ccaacttctt tcgaatttcg
     5521 ggaatcagta ttttttgttc cgcttgttca ttagcaataa tgtcgcttgc agctccgtga
     5581 taggtacaca aaaaagtatc tgttgcaatt ggtgaacgat ctacatgata cgaatacaca
     5641 tcggtggata tgaaatcgaa ttcatcgtca cgctcataac atttaagcaa attaagagag
     5701 ggcgaagctc caaaatcgga caataatcgc caatcattca agatgatttc ccttgccaat
     5761 tttccttttt tcgatagttg aagtgatagt aaatcctcag gatcaacttc ggttatgttt
     5821 tcttttagtt gcagttggcc tacaatctcc ttgaaatcgc cttccaaatt tcggtaccag
     5881 cacagtgcat tcatttcttc cttaaaatgt gtgtttacca gttcagaaaa agtgtacacc
     5941 acccctattt ggcggttatc agaaaatgta ttgctcataa atatgctatc agaaacaggt
     6001 tcgtttattt agtgcaaaat tatgaaatta aagctcaaca acggcacaat caactcaagc
     6061 ataccaacag actttaaatt tgtgaaaatt tgtggaattt gtggcgaaaa cttttaaaag
     6121 agaatgccgt gttctacgta aaacttcata aaaacacaga acctcaactt aattaatcta
     6181 aagcagagtc aagaaatctt tatcaatacc gcaataactc cagtaattct ttttcaaatc
     6241 gtccttttgg caaatattgg tcttccaagg ctttggtaaa cggaatggga ctatctaaac
     6301 tcgccacacg ttttacagga gcatcaagat attggaaaca attttccata attaaggccg
     6361 aaatatcgct ggctattcca ccaaacatag agtcttcttg caaaataata gctcgtcctg
     6421 ttttcttcac cgaagcataa atggcttcgg catccaaagg ttgcaaagtt cttaaatcca
     6481 aaacatctgc tgatatttca ggttttttag ccaaagtttc caatgcccaa tgcacggcag
     6541 caccataagc aataatcgta acttggtttc cttccttcaa caaggcagct ttccccaacg
     6601 gaattgtgta ataattcgtt ggcacctctt gatgaatgct tcggtacaat tgtttgtgtt
     6661 caaaaaacaa aacaggattc gggtcgttta tggattcgtt caacaagcct ttggcatcat
     6721 agggaaacgc agggtaaact accttcaaac cgggtgtttt tgtaaaccaa gcttcattag
     6781 tttgcgaatg aaaagggcct gcttgcgttc ctgcaccaca aggcattcgt accacaacat
     6841 cggctttttc ttgccaacga taatgcgttt tggctaataa attcacaatc ggattgaatc
     6901 cggtagaaac aaaatcggca aattgcattt cgacaatcgc cttatatcca ttgatagaca
     6961 aacccattgc tgccgaaact acggcgcttt cgcaaatggg tgtattgcga acgcgttctt
     7021 tgccaaactg ctcaacaaaa ccttcggtaa tcttgaaagc acctccatat tcggctatgt
     7081 cctgacccat aataaccaag ttatggtgtc gttccatcga ttgtttcaaa gcattggaaa
     7141 cggcatcaat aaatcgaata tttttggttt cttctgaagg aataacttct tgaaattgaa
     7201 atggtttgta aacatcattt aattcatcgt cataagtagc ttctatttca gattcggtat
     7261 tggcaagagc ccaattttca tcaattgctg ctttgatttc ggtatgcaaa agagcatcta
     7321 aatcatcaga aagaatttcg ttttcagata aaaatttcct gtaattttct actggatctt
     7381 tggtagtcca aacagcaata agttcttgag gaacatattt actgccactc gcctcttcgt
     7441 gtcctcgcat tctgaaagtc ttgaattcta ataaaacagg acgaggattt ttgaccatcg
     7501 aagctttcaa ttccgataat ttatgataca cttcaagaat attattcccg tcaataatat
     7561 gactttctat accatacccc actcctttgt cggctatatt ttcacaacga aactgctcat
     7621 ttgtaggagt tgaaagtccg tagccattgt tttcaacaat gaatagtacg ggcaattccc
     7681 aaacggctgc aatattcagc gcttcgtgaa aatcaccttc ggatgtagct ccttcacccg
     7741 taaaaacagc tgttactttt ccgttttgat ttaatttatt ggccaaagca attccatcag
     7801 caatccccaa ttgtggcccg agatgcgaaa tcattccaat aattttgaat tcttgtgtac
     7861 caaaatgaaa acttctgtcc cgacctttag taaaaccatc ggctttgcct tgccattgcg
     7921 aaaaaagtcg gtgcatcgga atatttcgtc ctgtgaatac acccaaattg cgatgcatag
     7981 gtaaaatata ttcatcggca tccaaaacag ctgtaacacc aacagcaata gcttcttgac
     8041 caatgccaga aaaccatttc gatacctttc cttgacgaat gagaatgagc attttttcct
     8101 cgattaaccg cggcattagt aaactggtgt acaaatacaa caattgctcg ttggtgagat
     8161 tttttctgtc aaaagtcatt gagattattt ttttattgtg tttcaaaagt ataaaaaaat
     8221 aacattccta agtcgaattg ttagattgtt tataagtagt catttattta ttttaggtga
     8281 ccgcagaaat attacaaaat ttaatacatt tataaaaatt ataaactaaa ccttatgaaa
     8341 aagaaactga ttattttatc tatgctttta ggcggattta cctattcgaa tgcacaaagt
     8401 atcgggacgc ttacaaaagc agctacttca agcgctactc aacaagccac ttctgcagca
     8461 gcaaactctt ttgatgttgc tggaatttca aatcaagtaa tgggagttct ttcgccaaaa
     8521 ttaaaattga ccgatgccca aaaaccattg gtaaattcat tagtaaatga aactttaaac
     8581 aaaaagaaaa gcattttacc gactgcactt tcagacaaag caggttatgc gtctaaaatg
     8641 ttaggaatca gcaatggttt tacttcaaaa atgaaaggac ttattaaacc tgaacaatac
     8701 acggcgcttt tgggtatctt gccaaaatca gcttcatcaa catctgcttt ggcaaaaatg
     8761 ttgttttagt attaaaaaaa aatagatttc tatttaaacc gagactttgt ttcggttttt
     8821 tttatccaaa aacttaaagc gaaatagtga actgactatt aaccataact gtttgaagtc
     8881 aaaacaagaa aaggaatgtt ataaataaaa agaggatgct aattaaaaca tcctcttcgg
     8941 agtaaattaa agttagaaca aataatttaa tctttaacgc ctaatttttt caaaatatcg
     9001 tccatcaaac accacttggt gagggatgat tgtaataaat tggctcccac aaaagcggta
     9061 aaccacaacc agttttggtt gacataaata gccaatagca agctaatcag gataaacgtt
     9121 cctgctacag ctcttacgat tctgtttttc atattcttta agattaaatg atttagttac
     9181 tacatctttc gatattcaac acggcaacgt gaatatttga ttttgaaact tgttcggtat
     9241 taaaatcgtt taccatttcg aaaaggccat cgactttttc ttctttgaca aaagcataga
     9301 acaaaacaga ttctgtttct tgcctgtcag tagcaaacca attggcttcc aaagcgtctt
     9361 cggaattgtc tttaaatcct tttacatctt tgtatgaaaa ggatttaact tggacctttt
     9421 taagaatttg tttgatgtct ttttcaaatt cccggactgc ggttattaat aatagtttca
     9481 ttttttttag tttaaaggtt taagattgtt taaaggttta agtgtttaaa cctttaaaca
     9541 agttttcact cattaggagt tcctatctcc cctcctttgg aggggtcggg ggaggtttcc
     9601 catttttttc tttcggtgat ataataaata atcggcacaa caagcagggt caaaacagtc
     9661 gaaactactg ctccaaaaac cagcgatatc gccaatcctt gaaaaattgg atcaaataat
     9721 ataatcgatg ctccaataac taccgctcct gttgtcaaca aaatcggagt tgttcgaaca
     9781 gcacccgctt cgataatggc ttgttttaat ggcacgcctt cgttgagtcg gatttcaata
     9841 aagtcaataa gtaaaaccga atttcgaacc ataacgcctg ccaaagcaat ca
//
LOCUS       DAOSNN010000076         9797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_6913_length_9797_cov_5.611579, whole genome shotgun sequence.
ACCESSION   DAOSNN010000076 DAOSNN010000000
VERSION     DAOSNN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6913_length_9797_cov_5.611579"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1050)
                     /locus_tag="PLQ70_05440"
     CDS             complement(187..1050)
                     /locus_tag="PLQ70_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQE34093.1"
                     /translation="MRQLKITKQVTNRETASLDKYLQEIGKVDLITADEEVELAQKIK
                     AGDQRALEKLTKANLRFVVSVAKQYQNQGLTLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGSINKINKMYALLEQSNERPPTAEEIAK
                     ELDMTVNDVRESMKNSGRHLSMDAPLVEGEDSNLYDVLRSGESPNPDRELIQESLRTE
                     IERSLETLTPREADVVRLYFGLGDQHPMTLEEIGETFDLTRERVRQIKEKAIRRLKHT
                     SRSKILKTYLG"
     gene            complement(1211..2515)
                     /locus_tag="PLQ70_05445"
     CDS             complement(1211..2515)
                     /locus_tag="PLQ70_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34094.1"
                     /translation="MENKKDIGKIFREKLNDFEKEPNDSVWNSIHAELQEKKKRRIIY
                     IPFWMKTAGILSLVVLISIFSIDAFLEKGQFSAPNESNQDILKSKSFEKSNPNKENAV
                     NTESLILTDASNNLISNKKSGQTSSNEGNLKKNKKKFSLKKGLTESKNNSTITDDSTV
                     KGSENNPLSAVENISDKNETNDKMLDSLKLKKEDKPELKIALEDKKDSLSDKKSGLYV
                     FVYGSPTYSGSLSKNSPLDNRLTNNPKTTVLNWNYGVYLGYESGYKWSLRLGIGKMNM
                     SFITANAAVNTVDYRNINYFKNTTNATIYTDTNFSETMTITQDVSYTEIPLEIKYNFY
                     DKKFGLSSIVGFSYLFVDENVISIKTSNGSNFDIGTTKNLLESTFSINAGFEMYYKLT
                     KKLKLNVEPLFKYHPIDYKKNSNPVNSYSFGIQTGLQFSFSK"
     gene            complement(2505..3053)
                     /locus_tag="PLQ70_05450"
     CDS             complement(2505..3053)
                     /locus_tag="PLQ70_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQE34095.1"
                     /translation="MNQDQLIKGCIQQNSKAQEELYNRYKANLFGLSLKYCSNETEAE
                     DNLHNAFIEIFTNIKNFNAKGTFEGWIKRITINKAIDSYKRTYKITALENDYYEEIIV
                     EDLEIDLSLDFLLSIIQELPNQYRIVFNLYELDDYSHQEIAQMLSISEGTSKSNLHRA
                     KLLLKEKITSKINSINNLSNGK"
     gene            complement(3158..4099)
                     /locus_tag="PLQ70_05455"
     CDS             complement(3158..4099)
                     /locus_tag="PLQ70_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HQE34096.1"
                     /translation="MKHHVTLFKALIFLLFISCHNEPVGMMPVDVLEEDISINDPKKT
                     GCNVGSCVGDVESPKLLDSILPNLNLPQNFDLSSLMPPVRSQGGQGSCVSFATTYYLK
                     SYQEKVQNKYDFITYDDVMSPAFVYNFSKPVGDCGLGSCIENALYVLKTKGTNTWSEF
                     PYDPFNCNKKPNVALMESALKHRISKSYKVNSFVKDNQNYKFLDIAKTLIHQGNPIII
                     AIKIDRNFVDNLPKKTDNLYVYEKYDFEKNYGNHAMLIVGYNDELKAFKVVNSWGTEW
                     GDKGYCWISYDFFREPTDLKFQLGILGAYIAYDKPNL"
     gene            complement(4280..4621)
                     /locus_tag="PLQ70_05460"
     CDS             complement(4280..4621)
                     /locus_tag="PLQ70_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34097.1"
                     /translation="MTVNIAFMTIEITKREKESTFEEQNNTRIIMTVSNTHFPMLKLI
                     SLSKKVITNPYAGVLMSLLLILPSLYIILEDITIIRKEYFFLAIGIPLYIKSLNKIFD
                     EILNSGNDKFK"
     gene            complement(4707..5453)
                     /locus_tag="PLQ70_05465"
     CDS             complement(4707..5453)
                     /locus_tag="PLQ70_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQE34098.1"
                     /translation="MDNINVLIIEDTPEQSDALSKVLLANKYNIVGVARSYTEALKLF
                     YELPIDIIVIDVFLDGKPDGITFAETINIIPNASKPFVFLTSSQDRQIFERAKLTKPF
                     SFLMKPFNELEILYALEMAVEKFYEQNNVFLSEEQDTVISNDYLFIKKKNALKKVALN
                     DILYIEVEERYCNIITEKEKFVILISLTKISDLLDKSKFIRTHRNIIVNSDKIEEIIL
                     ADNLIILKGNHKINLSDTYKDFIKKMNILS"
     gene            complement(5446..6816)
                     /locus_tag="PLQ70_05470"
     CDS             complement(5446..6816)
                     /locus_tag="PLQ70_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HQE34099.1"
                     /translation="MVSLRDFNKALIYFQIAEKQAIKTNDIQCLADVYSGFGTCYIFL
                     KQHQKSEKYLLKNLQLNEKLQNNDRICSSYMNLANLYYEQYKDELALIYFKKSYQISK
                     KIKAFDKKRDASINMAVFEENRGNFKQALVYRKESEQWKDSLNNQNKIWAVADFEKKF
                     AVAQKQKQIKVLEVENQLKNSQRNWLFFFAISMFLLLTAGVYFYAQKVKNAKIILLQK
                     NKLDELNATKDQLFSIVSHDLRSSVNALKTSNSKLSATLETKNYDELNQLIIQNSGIA
                     NGAYSLLDNLLHWALLQTRQLYFHKESVHLFSIVQQIEYNYKSLLLEKSITFENSVSR
                     NIFIFVDLDSLKIVLRNLLDNAIKFSNENGKISFYSVETHTDFCQLVLEDTGLGMSVN
                     TINELLADNELLAKKSNSEIIGTGLGMQLCKQMIKKNDGTLAIESEVNKGTKMILSFP
                     KTNQNG"
     gene            complement(7194..7583)
                     /locus_tag="PLQ70_05475"
     CDS             complement(7194..7583)
                     /locus_tag="PLQ70_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34100.1"
                     /translation="MALKVIQNTKINTGDFIFSSPETVTYPVAPLISAKFYSPNGVLT
                     LKIRATLYINSEDPNNPTVEAPAEINNSLQLYFDYNFSEETPESCDVWYVELDYTSDT
                     VEQITSITSFLRDIDPETSRGTTTNIP"
     gene            complement(7756..>9797)
                     /locus_tag="PLQ70_05480"
     CDS             complement(7756..>9797)
                     /locus_tag="PLQ70_05480"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQE34101.1"
                     /translation="VVVQMGNAMLLATVVSARKASPGVDFLPLTVDYREKFAAGGRFP
                     GGFFKREARPTDGEVLTMRLVDRVLRPLFPDDYHAETQVMIQLMSHDENVMPDALAGL
                     AASAALAISDLPFSTLISEVRVARVDGKFIINPSRAQLELSDIDMMIGASTDSIAMVE
                     GEMKEISELEMVEAIKFAHEAIKVQIVAQERLVAQVGKKETRTYELEKEDQAIYAKVK
                     AAAYDKIYAIAKVGSGKHERSAAFDAVKEEVKALFTAEELAENGDLVGKYFYKTNKEA
                     VRNVILDLGLRLDGRKTTEIRPIWAEVDYLPSVHGSSIFTRGETQALATATLGTSREA
                     NQIDSPSEQGEEKFYLHYNFPPFSTGEAKPLRGTSRREVGHGNLAQRALKNMIPADCP
                     YTIRIVSEVLESNGSSSMATVCAGTLALMDAGIQMIKPVSGIAMGLITDGDRFAVLSD
                     ILGDEDHLGDMDFKVTGTADGITACQMDIKIEGLKYNIMEEALAQAREGRLHILGKIT
                     DTLAAPKADVKAYAPKIITRTIPGNYIGALIGPGGKVIQELQKATGTTIVINEVDEQG
                     VIEILGTDPAGIEAVLAKIQSITFKPQMGEAYEVKVIKMLDFGAVVEYTAAPGNEVLL
                     HVSELAWERTENVSDVVNMGDVFQVKYLGLDPKTRKEKVSRKALLQRPPREEKKE"
BASE COUNT         3007 a         1685 c         1490 g         3615 t
ORIGIN      
        1 cgagcgaagc gaacagactg gagttttatt tgccacgcaa aaaataaaac tccggtaatc
       61 agccggagtt tcaatcatca atcaaaaaac gaacagttaa tcagaccgtc gttaactttg
      121 aaattccaat attaaaaatt ccaaattcca aaaaatgaga ttggagtttg gaattttatt
      181 tggaatttat cctaaatagg tttttaatat tttacttctt gaagtatgtt tcaatcttcg
      241 gatggctttt tctttaattt gacgtacacg ttcacgagtt aaatcgaaag tttctcctat
      301 ttcttctaat gtcattgggt gttgatcgcc taaaccaaaa tacaaacgaa ccacgtcagc
      361 ttctcttgga gtcaatgttt ctaatgaacg ctcaatttcg gtacgcaatg attcctgaat
      421 caattctctg tctggatttg gagattcgcc tgaacgcaat acatcataaa ggtttgaatc
      481 ttctccttca accaatggtg catccataga aaggtgacga cccgagtttt tcatagattc
      541 tctaacatca ttcacggtca tatctaattc tttggcaatt tcctcagcag ttggcggtct
      601 ttcattagat tgctctaaca aagcatacat tttgttgatt ttattgatag acccaatttt
      661 gtttaaaggc aaacgaacga tacgagattg ttcagccaaa gcttgcaaaa tcgattgacg
      721 aatccaccaa acggcatacg aaatgaattt gaaaccacga gtttcatcaa aacgttgagc
      781 cgctttaatc aaacctaagt taccttcgtt aatcaaatcg ggaagtgtta aaccttgatt
      841 ttggtattgt tttgccaccg aaaccacaaa acgcaaattg gctttggtta gtttctctaa
      901 ggctctttga tcaccggctt ttatcttttg tgccaattct acttcttcat cggcagtgat
      961 caaatccact tttccaattt cctgtaagta cttgtctaat gatgcagttt cacgattggt
     1021 tacctgcttg gtgattttta gttgtctcat gtttttgtct cctcaatttt taagtgtacg
     1081 agtggttata cgtgtggagt ttcaaaaaag ttacaaagga ttcaaaaaaa atcaaaatat
     1141 tattcctcaa ataagaatta agcaatattt aatccctttt tagataaaat tagtttgact
     1201 taaatctcat ctattttgag aacgaaaact gcaatccagt ctgaattcca aaggaatatg
     1261 aatttactgg attactattt tttttataat ctattggatg atatttaaat aaaggttcaa
     1321 catttaattt taattttttt gtcaacttat aatacatctc aaaacctgca ttgatactaa
     1381 aagtgctttc taataaattt tttgttgttc cgatatcaaa attacttccg tttgacgttt
     1441 tgatagaaat gacattttca tcgacaaaca aataactgaa tccaactatt gagctgagtc
     1501 caaatttttt atcataaaaa ttatatttta tttcgagtgg aatttctgta taggaaacat
     1561 cttgagtaat tgtcattgtc tcagaaaaat tagtatcagt ataaatagta gcattggttg
     1621 tgtttttgaa ataatttata ttccgataat ctactgtgtt tactgctgca tttgctgtaa
     1681 taaaactcat attcattttg ccaattccca aacgtaaact ccatttataa cctgattcgt
     1741 atcctaaata cactccgtag ttccaattca acactgttgt tttaggatta tttgtcaaac
     1801 gattgtctaa cggagaattt ttagacagcg aacctgaata agttggcgag ccataaacaa
     1861 aaacataaag cccacttttt ttatctgata aactatcttt tttgtcttct aaagctattt
     1921 taagttctgg tttatcttct ttttttaatt ttagactatc caacattttg tcattagttt
     1981 catttttgtc actaatattt tctacagccg aaagtggatt gttttcggat ccttttacag
     2041 ttgaatcatc cgtaatagtc gaattatttt ttgattctgt caaacctttt tttaggctga
     2101 acttcttttt attctttttt aaatttcctt catttgatga tgtctgccca ctttttttat
     2161 ttgaaattaa attattagaa gcgtctgtta gtatcaaact ttcagtgttt actgcgtttt
     2221 ctttatttgg attacttttc tcaaaacttt ttgattttaa gatgtcttga ttggattcgt
     2281 taggagcaga aaattgtcct ttttccaaaa aagcatcaat tgaaaaaatc gaaattaata
     2341 ctaccaatga cagtattccg gctgttttca tccaaaaagg gatataaata attctccttt
     2401 ttttcttttc ttgtagttcg gcatgaatag aattccaaac agaatcattt ggttcctttt
     2461 caaaatcatt tagtttttct ctaaatattt tacctatatc ttttttattt tccattgctt
     2521 aaattattta tggaattaat ttttgaggta atcttttctt ttaaaagtaa tttggcacgg
     2581 tgtaaattag attttgatgt tccttcggaa atagacaaca tttgtgcaat ttcttgatga
     2641 gaataatcat ctagttcata caagttaaaa actattctat attgatttgg caactcttga
     2701 atgatggaaa gtaagaagtc tagcgacaaa tctatttcca gatcttcaac aataatttct
     2761 tcataataat cattttctaa tgcggttatt ttatacgttc ttttataact atcaatagct
     2821 ttgttaatcg tgattctttt tatccaacct tcaaaagttc ctttagcatt gaaattcttg
     2881 atgtttgtaa atatttctat aaaggcatta tgcaaattgt cttcggcctc ggtttcattt
     2941 gaacaatatt ttaaactcag cccaaaaaga tttgctttat accggttgta taattcttct
     3001 tgtgccttgc tgttttgctg gatacagcct tttataagtt ggtcttgatt caaaaaaaga
     3061 cagagttaaa tgattctaaa tataacaatt aatctatacg ttactgatta cttttttgac
     3121 taagttgttc aattcaaata caacactttt tcgttcttta taagtttggt ttatcataag
     3181 caatgtaagc acctaagatg cctaattgaa attttaaatc cgttggttct ctaaaaaaat
     3241 cataacttat ccaacaatat cctttgtctc cccattccgt tccccatgaa ttcacaactt
     3301 taaaagcttt taattcatca ttatacccta caatcaacat ggcatgattc ccgtaatttt
     3361 tctcaaaatc atatttttca taaacataca aattgtccgt tttctttgga agattgtcaa
     3421 cgaagtttct gtctattttt atggctataa ttattggatt tccttgatga attaacgttt
     3481 ttgcaatatc caaaaactta taattttgat tgtccttcac aaaagaattg actttatatg
     3541 attttgaaat cctatgcttt aatgcggatt ccataagagc tacatttggt tttttgttgc
     3601 aattaaatgg gtcgtatgga aattcactcc aagtatttgt tccttttgtt tttaaaacat
     3661 ataaagcatt ttctatacaa gatcctaagc cacaatctcc aacgggtttg gaaaaattat
     3721 aaacaaaagc tgggctcata acatcatcgt atgttataaa atcatattta ttttgtactt
     3781 tttcttgata gctttttaaa taatatgtag ttgcaaatga aacacaacta ccttgccctc
     3841 cctgacttct tactggtggc attagtgatg acaaatcgaa gttttgtggt aaatttaaat
     3901 taggaagaat cgagtccaaa agttttgggc tttctacatc acctacgcaa ctcccaacat
     3961 tacatcctgt tttttttgga tcatttatag agatatcttc ttctaacaca tcgactggca
     4021 tcatacccac cggctcgtta tgacaactta taaaaagaag aaaaattaat gctttaaata
     4081 gggttacatg atgtttcatt ttcaaaattt gaatgttgct ataactcata gtcattataa
     4141 actaaaaaag gttgcgttga acacaaagaa ttatttttaa taaaaattaa aaaacccgtt
     4201 tcaaactaat gaaacgggtt ctactaaaaa aacataaaaa aaacgcaaat tttaagttaa
     4261 gtcatcgatt aattctgatt tatttgaatt tatcattacc actattcaat atttcatcaa
     4321 aaattttatt caatgacttt atatataacg gaattccaat ggctagaaaa aaatactctt
     4381 ttctaattat tgttatgtct tctaaaatta tgtataaact cggaaggatt agcaacaacg
     4441 acattaaaac tccagcatat ggattagtta ttactttttt cgaaagtgaa attaatttta
     4501 acatggggaa gtgtgtattt gatactgtca tgataattct agtgttattt tgttcctcaa
     4561 atgtactttc cttttctctt ttggtgattt caattgtcat gaaggcaata tttactgtca
     4621 tgaaatttcg attttggtta taaacaaaaa atcctctcgg caaggagaag atttaatgaa
     4681 ttattaggtt tgaaaaatga ctttcgttaa gacaaaatat tcattttttt tataaagtct
     4741 ttataggtgt cgctaagatt tattttgtga ttccctttca gaataatcaa gttgtcggca
     4801 aggatgattt cttcaatttt atccgaattc acaataatat ttcgatgcgt tcttatgaat
     4861 ttgcttttgt caagtaaatc actgattttg gttaatgaaa ttaagatgac aaacttttct
     4921 ttttcggtta taatgttaca atagcgttct tcaacttcaa tataaagaat atcgtttagg
     4981 gctacttttt tgagggcatt tttctttttt ataaacaaat aatcattgct aattacggtg
     5041 tcctgctctt cgctaagaaa gacattattt tgttcgtaga atttttctac cgccatttct
     5101 aaggcataga gaatttccaa ttcgttgaac ggtttcatca aaaaactgaa tggtttggtc
     5161 aatttggctc tttcgaaaat ttgtcggtct tgcgaactgg tcaaaaacac aaagggtttg
     5221 gatgcgtttg gaataatatt gatggtttcg gcaaaggtaa ttccgtcggg ttttccgtca
     5281 agaaaaacat ctattacaat gatatcgatg ggcagttcgt aaaaaagctt aagtgcctct
     5341 gtataacttc tggcaacgcc tacaatattg tatttgttag ccaataatac tttgctaagc
     5401 gcatcacttt gctctggcgt atcttcgatt atgaggacat taatattatc cattttgatt
     5461 tgtttttgga aacgaaagta tcattttagt tcctttgttt acttcacttt ctatcgctag
     5521 tgttccgtca tttttcttaa tcatttgttt gcacaattgc ataccaagtc ccgttcctat
     5581 aatctctgaa ttactttttt tagctaataa ttcattatct gctaataatt catttatagt
     5641 gttgacactc attcctaatc cagtatcttc gagtacaagt tgacaaaaat cggtatgagt
     5701 ttcgactgaa taaaagctga ttttaccgtt ttcgttcgaa aattttatgg cattgtctaa
     5761 taaattacga agcacaattt ttagcgaatc aagatccacg aagataaaaa tatttctgga
     5821 aaccgaattc tcaaaagtaa tcgatttttc taacaacaaa gatttgtagt tgtattctat
     5881 ttgttgcact atcgaaaaca aatgcaccga ttctttatga aaatacaatt gcctggtttg
     5941 caataaagcc caatgtaata aattgtccaa caaactgtag gctccgttgg caataccgct
     6001 gttttgaatg atgagctggt ttaattcatc ataattcttg gtttctagcg ttgcagataa
     6061 tttagaatta ctagttttta aagcatttac cgaagaacgc aaatcatggc ttactattga
     6121 aaaaagttgg tctttggtag cgttaagttc atcgagtttg tttttttgaa gcagaataat
     6181 tttagcattt tttacttttt gagcataaaa ataaactcca gctgttaaca gcaaaaacat
     6241 actgattgca aagaaaaaca accaatttcg ctggctattt tttagctggt tttctacttc
     6301 aagtactttg atttgttttt gtttttgggc tacggcaaac tttttctcga aatcagccac
     6361 ggcccaaatt ttgttttgat tgttgagaga atctttccat tgctcagatt cttttctata
     6421 aactaatgct tgtttgaagt tacccctatt ttcttcaaaa actgccatat ttatagaagc
     6481 atctctcttt ttgtcaaaag cttttatttt tttggaaatt tgatatgatt ttttaaagta
     6541 aataagtgct aattcgtctt tatattgttc gtagtataaa ttggctagat tcatatagga
     6601 tgaacaaatc ctatcattat tttgcaattt ttcgtttaat tgtaaatttt taagcaggta
     6661 tttttcggac ttttgatgtt gttttaaaaa gatatagcat gttccgaacc cgctatatac
     6721 gtcagctaaa cattgaatgt catttgtttt aatagcttgt ttttctgcta tttgaaaata
     6781 aatcagagct ttgttgaaat ctcttaaact taccattgag gcacctaaat aaaaattttt
     6841 aaacgatgaa agttgaaatg gctctgatat taagaaaaat tctttttttg attctacata
     6901 aagttttttg taataaaaac taacacctct aaaatagtga caataattta ctaaaatttt
     6961 atttttacta gtagatagct gctttattga atatactaaa gtggaatccc aatctttttt
     7021 taaataaaaa gattgggctt ttaaaaaatt gggttcattc ttaaatttga ctgactcctg
     7081 atgaatatat ctattctcaa ataaagtaat ttctttggtt tgggagaaaa gtaaatatgg
     7141 aactattaaa agaaaccaaa tgaaatattt attatttttc tgaaacattt taattaaggg
     7201 atatttgttg tagttcctct agaagtttca ggatcaatgt ccctcaaaaa tgaagtgata
     7261 ctagtaattt gttcaactgt gtctgatgta taatcaagct ctacatacca aacatcacaa
     7321 ctttctggag tttcttctga gaaattataa tcaaaataca attgcaatga attgttaatt
     7381 tcagcaggag cttcaactgt agggttgttt ggatcttcag agttgatgta taaagtagct
     7441 ctaattttca gtgtcaacac accgtttgga ctgtaaaatt ttgccgaaat taaaggagcc
     7501 acaggatagg taacagtttc aggactagag aaaataaaat ctcccgtatt aatttttgtg
     7561 ttttgaataa cttttaaagc catagtaatt tattgtttaa gattgataag gtaaagattt
     7621 tttttaataa tgtataaaat tagtattgtt tttacaatta ctactacaaa aatatttttt
     7681 taacaacaaa aaacccgttt caaatgaatg aaacgggttt tcctataaat gaacctttag
     7741 taaactaaga tttgattact cttttttttc ctcacgtgga ggtctttgca aaagtgcttt
     7801 tctagatact ttttcttttc tagtttttgg atcaagacca aggtatttca cttggaaaac
     7861 atcacccata ttcactacat ccgaaacgtt ttctgtgcgt tcccaagcca attctgaaac
     7921 gtgaagtaat acttcgtttc ctggagcggc tgtatattct acaactgcac caaaatctag
     7981 cattttgatt actttcactt cataagcttc gcccatttgt ggtttgaaag tgatggattg
     8041 gattttggcc aaaactgctt caattccagc aggatcagtt ccaaggattt cgataactcc
     8101 ttgctcatct acttcgttga ttacgatggt tgttccagta gctttttgca attcttgaat
     8161 tacttttccg ccaggtccaa tcaaagcacc aatgtagttg ccaggaattg ttcttgtaat
     8221 aatttttgga gcataagctt taacatctgc tttaggagct gccaaagtat ctgtgatttt
     8281 tccaagaatg tgcaaacgac cttcacgagc ttgagccaaa gcctcttcca taatgttgta
     8341 tttcaatcct tcgattttaa tatccatttg gcaagcagtg attccgtcag ccgttccagt
     8401 tactttaaag tccatatctc ctaagtgatc ttcgtcacct aaaatatcag acaatacagc
     8461 aaaacgatct ccgtctgtaa tcaatcccat tgcaattcca gaaactggtt ttatcatttg
     8521 aattccagca tccattaagg ctaaagtacc tgcacaaact gttgccatag aagacgaacc
     8581 atttgactct aatacttcag atacaatacg aattgtataa ggacaatcag caggaatcat
     8641 gtttttcaaa gctctttgag ccaagtttcc gtgacctact tctcttcttg aagttcctct
     8701 aagtggtttt gcttctcctg tagaaaatgg agggaaatta taatgcaaat agaatttttc
     8761 ttcgccttgt tcagatggag aatctatttg gttcgcttct ctagaagttc ctaaagtggc
     8821 tgtagccaag gcttgagttt ctccacgtgt aaaaattgac gaaccgtgaa ccgaaggcaa
     8881 ataatctacc tcagcccaga ttggtctgat ttcggtagtt tttcttccgt caagacgtaa
     8941 acctaaatca agaattacgt tacgaaccgc ttctttattg gttttataga aatatttacc
     9001 cactaaatct ccgttttcag ctaattcttc ggcagtaaac aaagctttaa cttcttcttt
     9061 tacagcatca aatgcagcag agcgctcgtg ttttccagaa cccacttttg caattgcata
     9121 gattttatca taagccgcag cttttacttt agcgtaaata gcttggtctt ctttttctaa
     9181 ttcgtatgtt cttgtctctt tttttccaac ttgtgctacc aatctttctt gagcaacaat
     9241 ttgaacttta atcgcttcgt gagcaaattt aatggcttcc accatttcca attctgaaat
     9301 ttctttcatc tctccttcta ccattgcaat agaatctgta gaagcaccaa tcatcatatc
     9361 aatgtctgat aattctaatt gagcgcgact tggattgata atgaattttc catctacacg
     9421 ggctacacgc acttctgaaa tcaaagtaga aaaaggaagg tcagaaatag caagagctgc
     9481 tgatgcggct aaacctgcta atgcgtctgg cattacgttt tcgtcgtgag acattaactg
     9541 aatcatcact tgtgtttcgg cgtggtaatc atctgggaaa agcggacgca aaacacgatc
     9601 cactaatctc atggtcaata cttcgccatc tgtaggtctt gcttctctct tgaaaaatcc
     9661 accagggaaa cgccctccag ctgcaaattt ttcacgataa tctaccgtaa gaggcaagaa
     9721 atccactcct ggacttgctt ttcgggccga tactactgtt gcaagcaaca tggcattacc
     9781 catttgaact actactg
//
LOCUS       DAOSNN010000077         9639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7049_length_9639_cov_4.646494, whole genome shotgun sequence.
ACCESSION   DAOSNN010000077 DAOSNN010000000
VERSION     DAOSNN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7049_length_9639_cov_4.646494"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PLQ70_05485"
     CDS             complement(<1..665)
                     /locus_tag="PLQ70_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQE34102.1"
                     /translation="MLFTAFIFGLISSFHCIGMCGPIAMMLPVDRSNQAKKVTQILTY
                     HLGRLTAYATIGLVFGLVGRGLYLAGFQQKMSIFIGVAMIAIVLIPEKVFAEYNFSKP
                     VFKLISKIKNSLGSHFKNKSYKSLFTIGLLNGFLPCGMVYVALFGAIAMQNELYGVSY
                     MILFGLGTVPLMSGMVFLQSFLTVSVRNKIQKIIPFVAVIIGILFIFRGLGLGIPYIS
                     PSD"
     gene            complement(722..1171)
                     /locus_tag="PLQ70_05490"
     CDS             complement(722..1171)
                     /locus_tag="PLQ70_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQE34103.1"
                     /translation="MKINWGTGIVIAFALFISFILYFIIKVQTNVKYDNELVVEEYYK
                     KDAKYGDEMIRIQNAQDLKQKPVITNDSEGVAIVFPTEFAPKDIKGIVSFYRSSNKKF
                     DFVVPISLSNSALKVPKEKLLGGRWEINMEWQYEGKQYLTKETIYVK"
     gene            complement(1201..2619)
                     /gene="ccoG"
                     /locus_tag="PLQ70_05495"
     CDS             complement(1201..2619)
                     /gene="ccoG"
                     /locus_tag="PLQ70_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HQE34104.1"
                     /translation="MSKLPDEAFRDTIGTIDAAGNRKFIFPKKPSGKFYDYRKWVSYF
                     LLLILIANPFIKIDGNQFMMFNVLERRFNIFGFPFWPQDFYLFVISMIVGVVFVILFT
                     VIFGRIFCGWICPQTIFLEMVFRRIEFWIEGDRGAQIRLEKQGWDAEKIRKKAMKWTI
                     FLIISFIIANVFLAYLIGSDELIKMIEDGPASHISTLISLFIFTGVFYFIFAWFREQV
                     CIIACPYGRLQGVLLDNKSINVAYDFVRGEKEAGRAKFNKQEDRAASGKGDCIDCKQC
                     VNVCPTGIDIRNGTQLECVNCTACIDECDTIMEGVGLPKGLIRYASEDEIEKNAKFKF
                     TARMKGYSAVLFILVGILVGLMFLRTEVEAVFLRLPGQLFQHKGENISNIYTFKVINK
                     TNKDFNDIHFKLVGIKGTLKVVGEQDLKVPKQGMKGGTVFIEINRFLLESDKTNLEIE
                     VYEGNKKIETSKTSFLSPRSFD"
     gene            complement(3028..3966)
                     /locus_tag="PLQ70_05500"
     CDS             complement(3028..3966)
                     /locus_tag="PLQ70_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HQE34105.1"
                     /translation="MKKIIPSYIRVLVIFFAVFAAMEYFIDSGDKPAFIKFPMVSLFL
                     LVFLFLLIAIEVTLNAVDTITYQLMTEEQKAKLAEANELGIKGSPLFKSVSNFLIKPK
                     KVEDEGELLLDHDYDGIKELDNNLPPWWVYLFYICIAFAVIYLVRFEIMGGDNQETEL
                     LKEMEQAKKEIAEFKKTAPDLMDEKTVTLLTDAADLAAGKEIFTTNCVACHRADAGGQ
                     IGPNLTDDKWILGGGIKNVFHTITNGGRDGKGMIAWSKSGLKPKDIQLVASYVLSLQG
                     SNPKDPKAPEGEVWVDPDAPKTAAVAPVATDATQVK"
     gene            complement(4002..4169)
                     /locus_tag="PLQ70_05505"
     CDS             complement(4002..4169)
                     /locus_tag="PLQ70_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HQE34106.1"
                     /translation="MFEQIKHNMETIAGVAVFPILSLLIFFAFFVGLAIWVFSYKKDK
                     IDEMSQIPLND"
     gene            complement(4175..6364)
                     /gene="ccoN"
                     /locus_tag="PLQ70_05510"
     CDS             complement(4175..6364)
                     /gene="ccoN"
                     /locus_tag="PLQ70_05510"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HQE34107.1"
                     /translation="MEMQQFYYDNKIVKKFIYATILFGVVAMSVGLLEAFFYLFPNLT
                     DGISWLSYGRLRPLHTNAAIFAFVGNAMFAGIYYSLQRLLKARMFSDFLSNLNFWGWQ
                     LIIVAAAISLPLGYSTSKEYAELEWPIDIAIALIWVVFGINMIGTILKRRERHLYVAI
                     WFYIATFVTVAVLHIFNSLELPVSAMKSYSVYAGVQDALVQWWYGHNAVAFFLTTPYL
                     GLMYYFVPKVANRPVYSYRLSIVHFWSLIFLYIWAGPHHLLYTALPTWAQNLGVVFSI
                     MLIAPSWGGMINGLLTLRGVWDKVREEPVLKFFVVAITGYGMATFEGPMLSLKNVNAI
                     AHFTDWIIAHVHVGALAWNGFMAFGIIYWLIPRMTKTTLYSTKLANFHFWIGTLGIIF
                     YTLPMYVAGFTQASMWKQFNPDGTLTYGNFLETVTQIMPMYWMRAIGGTLYLTGMLVL
                     VYNIIQTVRAGEPVEDELAEAPELQTISSGRLKGEKFHPWLERKPIQLTILATIAILI
                     GGIIQIVPTIMVKSNIPTISSVKPYTPLELQGRDLYIREGCVGCHSQMVRPFRSEVER
                     YGPQGKAGEYVYDHPFLWGSKRTGPDLLRVGGKYNDNWHFNHFWSPQSISAGSIMPAY
                     KWLFDNKAMDISMTEKKMEAMKTLGVPYSEEQIANGQKDLRAQALKIEESLKSDPDFV
                     KSYDESKKKAEARGEKFVPMNEREIVALIAYIQRLGTDIKVKDNTNK"
     gene            complement(6394..6591)
                     /gene="ccoS"
                     /locus_tag="PLQ70_05515"
     CDS             complement(6394..6591)
                     /gene="ccoS"
                     /locus_tag="PLQ70_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQE34108.1"
                     /translation="MSVIYLLISISIIVAVFFFLAFVRAVKTGQYDDDYTPSVRMLFD
                     DELIKKECDENIQITENHLKP"
     gene            complement(6702..9080)
                     /locus_tag="PLQ70_05520"
     CDS             complement(6702..9080)
                     /locus_tag="PLQ70_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HQE34109.1"
                     /translation="MEVKNCFHCGLDLQKEDQIVFDEKVFCCNGCKTVYEIFSQNDMI
                     CYYDFEKSPGATPLDISGKYDFLDNESIVAKLLEFQEDSAAIVSLNIPHIHCSSCIWI
                     LENLQKLQEGISTSQVNFPQKKVRITYNPEVVSLKKIVHLLSSIGYEPYISLENYETG
                     KNNVDRSLTYKLGLAFFCFGNIMLLSFPEYFEVKEFWLDTYKPFFRWLIFALSLPSFL
                     YSASGYYVSAYKSIKSGLLNIDIPIALGIIIFFIRSTFDIIMDYGSGFFDSLTGLIFF
                     MLLGKMFQIKTYSFLSFERDFKSYFPIAITRINTDFSEESVPIYDVEKGNRLLIRNQE
                     LIPVDGILISDKAEIDYSFVTGEAIPIAKKSGDKLFAGGKQIGKVIEMEVLHSVSQSY
                     LTQLWSNDVFQKNVEQKHKSITDKVSHYFTPILLLIAFAGFGYWIFIDANIAFNVFTA
                     VLIVACPCALALTAPFTMGNVLRILGKKKFYLKNALVIEQLAQVDTIVFDKTGTITTN
                     KKSNISYEGITLSEDDLLVLKNVLRASNHPLSRMLYDFLPECKRILINDFEEITGKGI
                     QAIVDGILIKVGSAEFVSKNEENNIQQTSVHIKINETYFGKYIFNNQYREGLAALFKE
                     LSHTYQIKVLSGDNEGERTSLEAILPKSTELIFNQKPEQKLEFIKKLQQNCQNVMMVG
                     DGLNDAGALAQSNIGISISENVNVFSPACDAILDANEFNKMNYFLKLSKKSITTIKMS
                     FALSILYNIVGLSFAITGNLQPLVAAIIMPLSTVTIVSFVTIASNFYAKRLN"
     gene            9190..>9639
                     /locus_tag="PLQ70_05525"
     CDS             9190..>9639
                     /locus_tag="PLQ70_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQE34110.1"
                     /translation="MGKCEQCIVREISSLKALNKDELLKLAECKTSYTIKKGSPIFEE
                     GEIVNGIYCITTGACKLSKLSPNGKDQIIKLVKAGELLGQRSMISDEPANLSAVALED
                     MEVCFIPKSEVLSMFDSNNQFSMNMMKTICGDLKEASEHMISISQKTV"
BASE COUNT         3294 a         1809 c         1411 g         3125 t
ORIGIN      
        1 atatctgatg gagaaatata cggaatgcca agtcctaacc ccctaaagat aaacaatatt
       61 ccgataataa ccgcaacaaa tgggatgatt ttttgaattt tatttcgaac agaaaccgtt
      121 aaaaaagact gaagaaacac cataccactc atcaatggaa ccgtccctaa accgaacaaa
      181 atcatgtatg aaacaccata caattcgttt tgcatggcta tggctccaaa taaagctaca
      241 tagaccattc cgcagggcaa aaatccgttc aataaaccaa tagtgaacaa cgatttataa
      301 ctcttgtttt tgaaatggct tcccaaactg ttcttgattt tggaaatcaa tttaaaaacc
      361 ggtttcgaaa aattatattc ggcaaaaact ttctcgggta tcaaaacaat ggcaatcatt
      421 gctacaccaa tgaatatgga cattttctgt tgaaatccag ccagatacaa acctcgtccg
      481 accaatccaa aaaccaaacc aatagttgca taagcggtta atcttcctaa atgatacgtc
      541 aggatttgag taaccttttt ggcttgatta cttctatcta caggcaacat catagcaatt
      601 ggtccgcaca ttccgatgca gtgaaaacta ctgattaaac caaagatgaa agcggtgaag
      661 agcattgtga ttttagatta cagattttag attgcagact gaaatctgta atctgatgaa
      721 tttacttaac ataaattgtt tcttttgtca gatactgttt tccttcatat tgccattcca
      781 tattaatttc ccaacgacca cctaaaagtt tttctttggg tacttttaga gcagaattcg
      841 aaagcgaaat aggaacaaca aaatcaaatt tcttattgga tgatcggtaa aaagaaacaa
      901 ttcccttaat atcctttggg gcaaattcag ttggaaaaac aattgcaacc ccttcagaat
      961 catttgttat tacaggcttt tgctttaaat cttgggcatt ttgaatccga atcatttcgt
     1021 ctccatattt tgcatctttc ttataatatt cttctactac cagttcgtta tcgtatttaa
     1081 cattagtttg cactttaata ataaaatata aaataaacga aatgaataat gcaaatgcaa
     1141 ttacaattcc tgttccccaa ttaattttca taagttgttt tttgttatac taattcaaag
     1201 ttaatcaaaa cttcttggac tcaaaaagct tgttttactt gtttcaattt ttttattccc
     1261 ttcatacact tcaatctcta aatttgtttt atcagattct aataaaaatc tattgatttc
     1321 aataaacact gttccgcctt tcataccttg tttgggcact ttcaggtctt gttctccaac
     1381 tacttttagt gtacctttaa ttccaactaa tttaaaatga atatcattaa aatctttatt
     1441 ggttttatta ataactttaa aagtataaat attactgata ttttcgcctt tgtgttggaa
     1501 taattgtcct ggtaatctca aaaatacagc ttcaacttcg gtacgtaaga acattaagcc
     1561 tactaagatt ccgacaagaa taaacaatac ggctgaatag cctttcattc tagcggtgaa
     1621 cttaaacttg gcatttttct ctatttcatc ttcagaagca taacgaatca atccttttgg
     1681 caaacctaca ccttccatta ttgtatcaca ttcgtctata caagctgtac aattgacgca
     1741 ttctaactga gttccgtttc ggatatcgat tccagttgga cagacattaa cgcattgttt
     1801 gcaatcaata caatccccct taccagatgc tgctctatct tcttgtttgt tgaatttggc
     1861 tctaccagct tctttttcgc cacgaacaaa atcataagcc acattgatgg atttattatc
     1921 taataacacg ccttgtaacc ttccgtaagg gcaagcaata atacatactt gttctctgaa
     1981 ccaagcgaaa ataaaataga aaacacccgt gaaaataaac aatgaaatca atgtactaat
     2041 atgacttgct ggtccatctt cgatcatttt gatgagttca tcacttccta taagataggc
     2101 aagaaagaca ttggcaatta taaaagaaat aatcaaaaag atggtccatt tcattgcttt
     2161 ctttctgatt ttctcagcat cccaaccttg tttttctaaa cgaatttgtg cgcctcgatc
     2221 accttcaatc caaaattcga ttcggcggaa aaccatttcc aagaatatag tttgtggaca
     2281 aatccatccg caaaatattc tgccaaaaat tactgtaaaa agaatgacga aaactacacc
     2341 aacaatcatc gaaattacaa agaggtaaaa atcttgtggc caaaatggaa atccaaaaat
     2401 attgaatctt cgctccaata cattgaacat cataaattga ttgccatcaa tctttataaa
     2461 tggattggca atcaaaatta gcaacaaaaa ataactcacc cactttctat aatcataaaa
     2521 ttttccggaa ggttttttag gaaaaataaa tttcctattt cctgctgcat caatggtgcc
     2581 gatggtgtct ctgaaagctt cgtctggtaa ttttgacatt gttaaaattt taaaatttaa
     2641 aagtataaaa ttaacgtttt gaatttattt gctttgaaac ttatgttaca ttactccaaa
     2701 aagaattgct tttattttat aaaaactgct tctgaaacaa gatacaaacg tttccttata
     2761 acaatgaagt gatttagact tttcccattt gctaaaaaat ttgtcttttt cacccaattt
     2821 cagccttttt ttcgttccgt agcccagcta tgcatcttaa aaaagactca aattgggcac
     2881 aaaatcctaa attttcgctt aaacgtaaaa agtctaaatc acttcaatat atattcaatt
     2941 aaagttaaat tatatttcta aataattata caaaaaacaa atcctgaaat aattcaggat
     3001 ctgctagcat tatatgtatc gataatctta ttttacttgt gtagcatctg tagcaacagg
     3061 tgcaactgcg gcagtttttg gtgcatctgg atcaacccaa acttctcctt ctggtgcttt
     3121 tggatctttc ggatttgaac cttgcaatga caacacatag cttgctacta attgaatatc
     3181 tttaggtttc agcccacttt ttgaccaagc aatcattcct tttccgtcac gaccaccatt
     3241 tgtaatggtg tggaaaacat ttttaattcc tccacctaaa atccatttat catcggttaa
     3301 gtttggtcca atttgccccc cagcatcggc tctatgacaa gccacacaat ttgtggtaaa
     3361 aatttctttt cctgctgcta aatctgccgc atctgtcaat aaagtaaccg ttttttcatc
     3421 catcaaatca ggagctgttt ttttaaattc agcaatttct tttttagctt gctccatttc
     3481 ttttagcagt tcggtctctt gattatcacc tcccattatt tcaaaacgaa ctaaatatat
     3541 tacagcaaaa gcaatacaga tatagaataa ataaacccac caaggcggta aattattatc
     3601 tagttctttg attccatcat aatcgtggtc taataacaat tctccttcgt cttcaacttt
     3661 tttaggtttg ataaggaaat ttgacacact tttgaatagt ggactaccct tgatacctaa
     3721 ttcatttgct tctgctaatt tagctttttg ctcttctgtc atcaattgat aagtgatagt
     3781 atcgactgca tttaaggtaa cttctattgc tatcaacaag aaaagaaata ctaataaaaa
     3841 gagagaaacc attggaaatt ttataaacgc gggtttatct ccagagtcga tgaaatattc
     3901 cattgcagca aatacggcaa agaaaataac aagaactctt atatatgagg ggattatttt
     3961 tttcatttta tatctatttt gaaaattata tattatttgt tttaatcgtt caaaggaatt
     4021 tgactcattt catcaatttt atctttcttg tatgaaaaaa cccaaatggc tagtcctaca
     4081 aaaaaggcaa agaaaatcaa taacgatagt attggaaata ctgcaactcc tgcaatggtt
     4141 tccatattgt gtttaatttg ctcgaacata attactattt attagtattg tctttcactt
     4201 tgatatccgt tccaagtctt tgaatgtaag caattaacgc tacaatttct ctttcattca
     4261 ttggaacaaa tttttctcct ctggcttctg cttttttctt gctttcgtca taacttttta
     4321 caaagtcagg atccgatttc aaactttctt caattttcaa agcttgtgct ctcaagtctt
     4381 tttgcccgtt ggcaatttgc tcttcagaat aaggaactcc aagggttttc atggcttcca
     4441 ttttcttttc agtcatagaa atatccattg ctttattgtc aaacagccat ttgtaagcag
     4501 gcataataga acctgctgaa atactttgtg gactccagaa gtgattgaaa tgccagttgt
     4561 cattgtattt accaccaact ctcaacaaat ctgggcctgt acgttttgaa ccccaaagaa
     4621 atgggtgatc atatacatat tctccagctt taccttgtgg gccataacgt tctacttcgc
     4681 tacggaaagg acgaaccatt tgagagtgac aacctacaca accttcacga atatataagt
     4741 cacgaccttg taattctaat ggtgtgtaag gttttacact tgaaattgtt gggatattcg
     4801 attttaccat aatcgtaggt acgatttgaa taattcctcc aattaagata gcaatggttg
     4861 ccaaaatggt caattgaatt ggttttcttt ctaaccaagg gtggaatttt tcacctttca
     4921 atcttccaga actaatagtt tgtaattcag gcgcttcggc taattcgtct tctacaggtt
     4981 ctcctgctct tacggtttga ataatattgt aaaccaatac taacattcct gtaagatata
     5041 gtgtacctcc aatagctctc atccaataca ttggcataat ttgagttact gtttcaagga
     5101 agttaccata agttaaggtt ccgtcaggat tgaattgttt ccacatcgat gcttgagtaa
     5161 acccagcaac atacatcgga agggtgtaaa atattattcc taaagttcct atccaaaagt
     5221 gaaaattagc taatttagtc gaataaagag tggttttagt cattcgaggt atcaaccaat
     5281 aaataatacc gaaagccata aatccattcc aagctaaagc tcctacgtga acgtgagcaa
     5341 taatccaatc cgtaaaatgc gcaatagcat ttacattttt aagggataac attggtcctt
     5401 caaaagtagc cataccataa cctgtaatgg caacaacgaa gaatttaaga actggttctt
     5461 cacgaacttt atcccaaaca cctcttaaag ttaaaagtcc gttgatcata ccaccccaag
     5521 atggagcaat caacataata gagaaaacaa ctcctaaatt ttgtgcccaa gtaggcaaag
     5581 cggtgtacaa taaatggtga ggcccagccc aaatgtataa gaaaattaac gaccagaagt
     5641 gcacaataga cagtctataa gaataaaccg gacgattggc tacttttgga acaaaataat
     5701 acatcaatcc taaataaggt gtagtcaaga aaaaggcaac tgcattatga ccataccacc
     5761 attgaactag tgcatcttga actcctgcat aaacagaata gctcttcatt gcactaacag
     5821 gcaattctaa actgttgaaa atatgtaaaa cagcaacagt aacaaatgta gcgatgtaaa
     5881 accaaatcgc aacatataag tgacgttctc ttctttttaa tatcgtacca atcatattaa
     5941 taccaaaaac aacccaaatt aaagcaatgg caatatcaat tggccactct aattcagcat
     6001 attctttgga agtgctatac cctaatggca aagaaatagc ggcggcaaca ataatcaatt
     6061 gccatcccca aaagttcaaa ttactcaaaa agtcactgaa cattctggct tttaataatc
     6121 gctgcaaaga ataataaata cccgcaaaca ttgcatttcc tacaaaagca aaaatcgccg
     6181 cattagtatg taaaggtctt aatctaccat aacttaacca cgaaattcca tcggttaaat
     6241 ttggaaagag ataaaagaag gcttctaata gcccaacaga cattgccaca actccaaaaa
     6301 ggatcgttgc gtaaatgaat ttctttacaa ttttgttgtc ataataaaac tgttgcattt
     6361 ccataaataa taattgttaa ttggtttaaa aattcaaggt ttaaggtggt tttcagtaat
     6421 ttgaatgttt tcatcacatt cttttttaat gagttcgtcg tcaaaaagca tgcgaacgga
     6481 aggggtataa tcatcatcgt attgacctgt ttttacagct cttacaaaag ctaaaaagaa
     6541 aaaaacagca acgataatac tgatggaaat tagtaagtaa ataacactca taaaaattaa
     6601 ttgatttggc aaatgtacta catcgattta tctcaaatta tgacaattat catatttttg
     6661 ggttttgtca aatttacaat aaaaaaactt cattaattaa tctaatttaa ccttttcgca
     6721 taaaaattac tagcgattgt tacaaaacta acaattgtca ctgtactcaa gggcattatt
     6781 atcgcagcaa ctaatggctg taaatttccg gttattgcaa atgataatcc tactatatta
     6841 tagagaatcg ataatgcaaa actcatcttt atagtggtga ttgatttctt agataatttt
     6901 agaaaataat tcattttatt aaattcattc gcatctaaaa tggcgtcaca agcaggcgaa
     6961 aaaacattga cattttctga aatcgaaatc cctatattac tttgcgccaa agctccagca
     7021 tcattcagac catcacctac catcataacg ttttgacaat tttgctgtaa tttttttata
     7081 aattccagtt tttgttctgg tttttgattg aaaattaact cggtgctttt tggcaaaata
     7141 gcttctaaag aagttcgttc tccttcgtta tcgccagaca aaaccttgat ttgatacgta
     7201 tgactcaatt ccttgaaaag cgcagctaaa ccctctctgt attgattgtt gaaaatgtat
     7261 ttaccaaaat aagtttcatt tatcttaata tgaaccgatg tttgttgaat attattttcc
     7321 tcatttttgc ttacaaattc tgctgaacca acttttatta gaattccatc gacaatagct
     7381 tgaatcccct tacctgttat ttcttcaaaa tcattaatta gaattctttt acattctggc
     7441 aaaaaatcat acaacattcg actcaatgga tgattggatg ctctaaggac attttttaaa
     7501 accagtaaat catcctctga aagcgtgatt ccctcataag aaatattcga ttttttattg
     7561 gttgtaatgg ttcctgtttt gtcaaaaacg atggtgtcaa cttgagctaa ttgctctatt
     7621 actaaagcgt ttttgagata aaatttcttc ttgcccaaaa ttcgcaaaac atttcccatt
     7681 gtaaagggag ctgttaatgc caatgcacaa gggcaagcta caatcaaaac cgctgtaaaa
     7741 acattgaaag caatattggc atctataaat atccaatatc caaaacctgc aaaagcaatg
     7801 agcaataata tgggggtaaa ataatggctc actttatctg taatggattt gtgtttttgt
     7861 tcgacatttt tctgaaaaac atcattactc caaagttgtg tcaaatagct ttgcgaaacc
     7921 gagtgcaaca cttccatttc tatcacttta cctatttgtt tgccgccagc aaaaagctta
     7981 tcgcccgact ttttagcaat tggaatggct tctccagtca caaaactata atcgatttcg
     8041 gctttgtcag aaattagaat tccatcaaca ggaattaatt cttgatttcg aatcaaaagg
     8101 cgatttcctt tttcgacatc ataaatagga acgctttctt cagaaaaatc agtgttgatt
     8161 cgagtaattg caattggaaa ataagattta aaatctcttt cgaaactcaa aaaactatag
     8221 gtttttattt gaaacatctt gcccaaaagc ataaagaaaa tcaaaccagt caaactgtca
     8281 aaaaaaccag agccataatc cattatgatg tcaaaagtac ttctgataaa aaatataatg
     8341 attcccaaag caattggaat atcaatattc aacaaacccg atttgatact tttgtaagcc
     8401 gaaacataat atccactagc cgaatataaa aaagatggca atgataacgc aaaaattaac
     8461 catctaaaaa acggcttata agtgtctaac caaaactctt tgacctcaaa atattcggga
     8521 aaagaaagca acattatatt gccaaagcaa aagaaagcca aacccagttt ataagtcaga
     8581 ctcctatcga cattgttttt gccagtttca taattttcta aactgatgta aggttcatat
     8641 ccaatagaac tcaataaatg aacaatcttt ttgagcgaaa caacttctgg attataggta
     8701 attcggactt tcttttgagg aaagttgact tgagaagtac taatcccctc ttgtagtttt
     8761 tgaagatttt ctagaatcca aatacaagaa ctacagtgaa tatggggtat attcaaagaa
     8821 acaattgcag cagaatcttc ttgaaattct aaaagttttg caacaatact ttcattgtct
     8881 aaaaaatcat atttaccact gatgtcaagc ggagtcgctc caggagattt ctcaaaatca
     8941 taatagcaaa tcatatcatt ttgactaaaa atttcgtaca cggttttgca accattgcaa
     9001 caaaaaactt tttcatcaaa gacaatttgg tcctcttttt gaagatccag cccacagtgg
     9061 aaacagtttt taacctccat aaatttttct gtttttacag cccacaaata tagtagctga
     9121 aaaaatttaa aatatgataa ttatcatata atttcgtaga tttgcaagtc aattttatat
     9181 ttttcttata tgggtaaatg tgaacaatgc atcgttagag agatcagctc attaaaagcg
     9241 ctaaataaag acgaactatt aaaactcgct gaatgcaaaa catcctatac aataaaaaaa
     9301 gggagtccca tctttgaaga gggtgaaatt gtaaatggga tatattgcat tacaactggt
     9361 gcttgcaaat tatctaaact aagcccaaac ggcaaagacc aaattataaa attagtaaaa
     9421 gctggggaat tgctaggaca acgttctatg attagtgatg aacctgcaaa tctaagtgct
     9481 gtagcgctag aagatatgga ggtttgtttt attccaaaaa gcgaagtttt gagcatgttt
     9541 gattctaaca atcagttttc aatgaatatg atgaaaacga tttgtgggga tttgaaagag
     9601 gccagtgaac atatgatttc tatttctcaa aaaacagtc
//
LOCUS       DAOSNN010000078         9564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7114_length_9564_cov_4.690504, whole genome shotgun sequence.
ACCESSION   DAOSNN010000078 DAOSNN010000000
VERSION     DAOSNN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7114_length_9564_cov_4.690504"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2015
                     /locus_tag="PLQ70_05530"
     CDS             <1..2015
                     /locus_tag="PLQ70_05530"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQE34111.1"
                     /translation="GKLATTPEANIIKLPNVSASVPQLKAAIAELQANGYALPNFPED
                     PQNEEEKAVKAKYSKVLGSAVNPVLREGNSDRRAPKAVKNYAKANPHSMGAWSADSKS
                     KVASMESGDFYGSEKSVTITEANDMKIEFVAKDGTVTVLKASTPLKSAEIIDSSVLNI
                     QKLRSFVAEAIKESKEKNILFSVHLKATMMKVSDPIIFGTIVEVYFADVFEKYATLFA
                     ELNIDTRNGLGDVYAKIAGNAKQAEVEAALNQAIENGPALAMVNSDKGITNLHVPSDV
                     IVDASMPAMIRTSGQMYNKEGKQQDTLALIPDRCYAGVFEAVIEFCKKHGAFDPRTMG
                     SCSNVGLMAQKAEEYGSHDKTFQIKEDGIVRAVDTNGIVLMEQAVEKNDIFRMCQTKD
                     APIQDWVKLAVNRARLTSLPTIFWLDNNRAHDRELITKVEKYLKDYDTTGLDIQILNP
                     IEATNFTMERMIKGLDTISVSGNVLRDYLTDLFPILELGTSSKMLSIVPLMNGGGLFE
                     TGAGGSAPKHVQQFVEEGYLRWDSLGEFLALGASLEYIGETLNNSKAFVLAETLDAAT
                     EKFLANDKSPARKVGQIDNRGSHFYLAMYWAEALGAQDKDAELKAIFAPIAAEFFANE
                     AKINAELIGTQGKPQNIGGYYQPNPELTSKAMRPSETFNSILAKIA"
     gene            2131..2550
                     /locus_tag="PLQ70_05535"
     CDS             2131..2550
                     /locus_tag="PLQ70_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQE34112.1"
                     /translation="MTAKKTITVETLIKAPIEKIWEFWTNPEDITKWCTASEDWHTTR
                     AENNLKTGGKFLTRMEAKDGSFSFDFEGIYTDVITNEKIGYTTLDDRKVDITFSQTES
                     GIQITETFEAENENPIEMQKMGWQAILNNFKTYIEKQ"
     gene            2641..3480
                     /locus_tag="PLQ70_05540"
     CDS             2641..3480
                     /locus_tag="PLQ70_05540"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQE34113.1"
                     /translation="MKKTIYPCLWFDGNAKASADFYCSIFKNSKIINNTPMVVRFEIE
                     GKLIMGLNGGPMFKINPSISLFVTCETNEEIESIWNQLSKEGTIMMPLDQYPWSEKYG
                     FVADKFGVCWQLMLGEIPPNGQKIMPCFLFVEEQYGKAQAAIKQYTSIFPNSEIQDLS
                     IDESEDPQQKGSLKFGNFKLQNELFAAMDGFGKHQFQFNEGVSLVVECDTQDEIDNYW
                     TKLTEGGSESQCGWLKDRFGVSWQIVPTILSELMSDPEKASRVVQAFMKMKKFNIETL
                     LNT"
     gene            3491..3856
                     /locus_tag="PLQ70_05545"
     CDS             3491..3856
                     /locus_tag="PLQ70_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQE34114.1"
                     /translation="MKTIFKDIDHYISVQTPDVQVLLEQMRQTIKKAAPKAEEVISYN
                     MPAFKYHGMLVYFAAYKNHIGFYATPTGHSQFKEELSIYKQGKGSVQFPLTKPLPLDL
                     ISRIVQFRMNENLIKSTKK"
     gene            3971..6373
                     /locus_tag="PLQ70_05550"
     CDS             3971..6373
                     /locus_tag="PLQ70_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE34115.1"
                     /translation="MISKKIIALLLFFLTTIDSFSQDTNSSELTKQKFTLSGTITDAN
                     SNETLIGVNVSIAELKTGVTTNEYGFYSITLPKGNYTLQIAYLGFQNMEEKIQLSQNF
                     KRNFKLQSSEQQLQEVVVTSNKKSTNIKTPEMSVNKLSMGTIKKMPVVLGETDVLKSI
                     LLLPGVTNAGEGQSGFNVRGGAADQNLVLLDEATIFNSSHVFGFFSVFNPDAIKDLKL
                     YKGGIPSRYGGRASSVLEIYQKDGNSNNFHMNGGIGLISSRLLAEGPIVKDKGSFLIG
                     GRTSYAHLFLKLADNDNSASFYDLNAKLNYKLNANNSLYVSSYFGRDFFNISKEFQNT
                     YGNSTFNLRWNHLFSERHFSNLSAIFSDYYYGLTLSSVGFDWQSSIKNFNIKYDLKYY
                     LNDKIKLSYGLNSIYYKFNPGTIEPIDANSSINFKQLDKKNAFEPSIYLEAEHQATQK
                     LSFMYGLRYSMFYRLGASTVNLYENNQAVTFNNQLKIYEKGVPTGTKSYGYNEVITSF
                     SNLEPRFSSSYVLDEKSSIKASYNRMVQYLQLVSNTASPTPLDIWTPSDQYIQPQIAD
                     QVAAGYFRNFKEDMYSLEVESFYKKVKNRIDYIDGADLIANNAIEQVILNGQLRAYGL
                     ELMLRKNSGKFNGWVAYTLSNSEQQTPGRSPNETGINNGNWYKSGYHKLHNLAVTANY
                     NLNPKWTFGGNFILQSGQPVTFPNGQYQYQGITVPSYGGRNENNLPLYHHLDLAATYT
                     PKPEKRKGWQSEWVFSVYNIYNRKNAASISFRENQTSGANEAVKLSIFGAVPSVSYNF
                     KF"
     gene            6378..7199
                     /locus_tag="PLQ70_05555"
     CDS             6378..7199
                     /locus_tag="PLQ70_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HQE34116.1"
                     /translation="MQILKYITLSLLLLSIISCEEVIDVDLKTETPRLVIDAAINWQK
                     GTDGSSQKIMLSTTTSFYNTIIPKVSGATVSVKSSENFNYIFTEIPNTGEYQCTTFKP
                     IIGETYTLTVVYKNETYTATETMQSVSSIYSIQQKNDGGFSGKDYEIEVSFKDPLVKN
                     FYMVKFFPDIYKAPSYQVIGDEYTNGNLKSWQFSDEDLKQGSTIAITHYGVSESYYNY
                     MNKLISVASSSGGGSPFQTPPATVRGNIVNQTNINNYALGYFSLSEVDYQNYIIK"
     gene            7333..7995
                     /locus_tag="PLQ70_05560"
     CDS             7333..7995
                     /locus_tag="PLQ70_05560"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HQE34117.1"
                     /translation="MSSHHIVRDDQEPALIIANGAACNPELLGQLLEWSPLVIVLDSA
                     MERVLELGIKVDVLLGDFDRGFDPNYYKEKQYPLEIVHTPDQNKTDLEKAFDYLIERE
                     IPAVNVVWATGKRADHTITNITNISRYRDLIKIVVLDDHSKVFLLPKKFEKWYPANTP
                     LSLIPVGHVTGIHSENLFYPLENDTLTIGYRTGSSNHVKEDGIVIIEHQEGDLLMMEC
                     WD"
     gene            8093..9046
                     /gene="rlmF"
                     /locus_tag="PLQ70_05565"
     CDS             8093..9046
                     /gene="rlmF"
                     /locus_tag="PLQ70_05565"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HQE34118.1"
                     /translation="MKLKPIIEKTNLHPRNQHRMGYNFEELIALCPELQPYVSINEHN
                     IKTIDFSNPQAVKALNKALLIANYDIQNWDIPSTNLCPPIPGRVDYIHYIADLLASNN
                     NGIIPEGENVQVLDIGIGANCIYPILGNTVYGWSFVGTEIDEKAIQNCKKIIEANPRL
                     MDYISLQLQTESRFIFKNIITPEDRFSLTICNPPFHNSAEEATKASIRKVNNLENTRT
                     TKPVLNFGGQNAELWCEGGELGFITQMIFESAKFPMQCLWFTTLVSKKENLSSLYKTL
                     NKVNVVEVKTIDMAQGQKTSRILAWTFQTEAQQKNWKFELE"
     gene            9110..9529
                     /locus_tag="PLQ70_05570"
     CDS             9110..9529
                     /locus_tag="PLQ70_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34119.1"
                     /translation="MHFSIIPFLLVSGYVVTISRSMLNERYKKRLILSVVSLILGIVF
                     EIFSKEEKRFSYIVFGLLPLMYLFYYEILRIIFMPLIGKFPYLPYREKIGAKVLGYDY
                     PKNRKVKLADYIFGMTLIFLPLITMLFLGILLNNYFK"
BASE COUNT         3379 a         1683 c         1652 g         2850 t
ORIGIN      
        1 taggaaaact agccacaact ccagaagcca atatcataaa actacctaat gtttcagctt
       61 ctgttccgca actaaaagca gctattgctg aattacaagc gaatggatat gccttaccaa
      121 actttcccga agatccacaa aatgaagaag aaaaagctgt taaagctaaa tattctaaag
      181 ttttaggttc ggctgtgaat ccagttttgc gcgaaggaaa ttctgaccgt agagctccaa
      241 aagcagttaa gaattacgca aaagccaatc cgcattcaat gggagcttgg tctgctgatt
      301 ctaaatcaaa agtagcctcg atggaaagtg gtgattttta cggaagtgaa aaatcagtaa
      361 ctattactga agctaatgat atgaaaattg agtttgttgc caaagacgga actgttaccg
      421 ttttaaaagc aagcacacca ttaaaatcag ccgaaattat tgacagttct gtattaaaca
      481 tacaaaagct aagaagtttt gttgctgaag ccataaaaga atctaaagag aaaaacattt
      541 tgttttcagt tcacttgaaa gcaacaatga tgaaagtttc ggatcctatt atttttggaa
      601 ctattgtaga agtatatttt gctgatgttt tcgaaaaata cgccacttta tttgccgaat
      661 taaatattga tacccgcaat ggtttgggtg atgtttatgc aaaaattgca gggaatgcaa
      721 aacaagccga ggtagaagct gcattaaacc aagcaataga aaacggaccc gctttggcta
      781 tggtgaattc agacaaagga attaccaatc ttcacgttcc atctgatgta attgtagatg
      841 cttcaatgcc tgcaatgatt cgtacttctg gacaaatgta taacaaagaa ggaaaacaac
      901 aagatactct tgctcttatt ccagatcgtt gttatgcagg tgtttttgaa gctgtgattg
      961 aattttgtaa aaaacacggc gcttttgacc caagaacaat gggaagttgt tcaaacgtag
     1021 gtttgatggc acaaaaagcc gaagaatatg gatcacacga caaaacattc caaattaaag
     1081 aagacggaat tgttcgtgca gtagatacaa atggcattgt tttgatggaa caagccgttg
     1141 aaaaaaatga tatttttaga atgtgccaaa ccaaagatgc tcctattcaa gactgggtaa
     1201 aactggccgt aaatagagct cgtttgacta gtcttccaac aattttctgg ttagacaata
     1261 acagagcaca cgacagagaa ttaattacta aggtcgagaa atacttaaaa gactacgata
     1321 caactggtct agatattcaa attctgaatc caattgaagc taccaatttt acaatggagc
     1381 gaatgattaa aggattagac accatttctg tttctggaaa tgtattgcgt gattatttaa
     1441 ctgatttatt cccaatctta gaactaggaa cttcttcaaa aatgttgtct atcgttcctt
     1501 taatgaatgg tggtggattg tttgaaactg gtgctggagg ttctgctcct aaacacgtac
     1561 aacaatttgt agaagaaggc tatttacgtt gggattctct aggtgaattt ttggcattag
     1621 gtgcttcttt agaatatatt ggagaaacat taaacaattc gaaagccttt gttttggccg
     1681 aaacccttga tgctgccact gaaaaattct tagcaaatga caaatctcct gctcgtaaag
     1741 taggacaaat tgacaaccgt ggatcacact tttatttggc gatgtactgg gcagaagctt
     1801 tgggagcaca agataaagat gccgaattaa aagccatttt tgctccaatc gctgccgagt
     1861 ttttcgcaaa tgaagcaaaa atcaatgccg aattaattgg aacacaagga aaaccacaaa
     1921 atattggtgg ttactaccaa cctaatcctg aattgactag caaggcaatg cgacctagtg
     1981 aaacatttaa ttctattttg gcaaaaattg cataaatgtt tttttacaat tatagaacac
     2041 taataaagac gactttcggg tcgtcttttt ttttatatat ttgaatgaac caaaaaatat
     2101 ccaacagatt ttaaaaacaa acttctgaaa atgacagcaa aaaaaaccat tactgtagaa
     2161 acacttataa aagctcccat cgaaaaaatt tgggaattct ggacaaatcc cgaagacatc
     2221 acaaaatggt gcactgcttc tgaagattgg cacaccaccc gagccgaaaa caatctgaaa
     2281 actggaggta aattcctcac cagaatggaa gccaaagacg gcagttttag ctttgatttc
     2341 gaagggattt atactgatgt aataaccaat gaaaaaattg gttacaccac tttggatgac
     2401 agaaaagtag atattacatt ttcacaaacc gaaagtggaa tccaaatcac ggaaactttt
     2461 gaagccgaaa acgaaaatcc cattgaaatg caaaaaatgg gttggcaagc tattttaaat
     2521 aacttcaaaa cttatattga aaaacagtag taacattaaa tcctaaaact atggcagaaa
     2581 caatgacctt ttaattgcgc caataaatca tagatcatct aattctaaaa tacaataaca
     2641 atgaaaaaaa caatttatcc ttgcttatgg tttgatggta atgcaaaagc atcagccgat
     2701 ttttattgtt ccattttcaa aaattcaaaa ataataaata acactccaat ggtggtgcgt
     2761 tttgaaattg aaggtaaatt gattatgggg ttaaacggtg gaccaatgtt caaaataaat
     2821 ccatccatat ctctttttgt cacttgtgaa accaatgaag aaatagaatc tatttggaac
     2881 cagctatcaa aagaaggaac tataatgatg cctttagacc aatatccttg gagtgaaaaa
     2941 tatggttttg ttgctgataa atttggcgtt tgctggcaat taatgctggg ggaaattccg
     3001 ccaaatggac agaaaataat gccttgcttt ctatttgttg aagaacaata cggcaaagct
     3061 caagcagcta taaaacaata cacttccatt ttccccaatt cagaaataca agatttaagt
     3121 attgatgaat cggaagatcc gcaacaaaaa ggaagtttga aatttggcaa tttcaagttg
     3181 caaaatgaat tatttgctgc tatggatggg tttggcaaac accaattcca gttcaacgaa
     3241 ggcgtttcat tagttgtaga atgtgacaca caagatgaaa tcgacaacta ttggacaaaa
     3301 ctaactgaag gcggaagcga aagtcagtgt ggctggctaa aagatagatt tggcgtttct
     3361 tggcaaattg taccaaccat tttaagtgaa ttgatgtccg atcccgaaaa agcatctaga
     3421 gtcgttcaag cttttatgaa aatgaaaaag tttaatatcg aaactttgtt aaatacctaa
     3481 aacaactatt ttgaagacca ttttcaaaga tatagaccat tatatttctg ttcaaactcc
     3541 agatgttcaa gttcttttgg agcaaatgcg gcaaaccata aaaaaagctg ctccaaaagc
     3601 tgaagaagta atcagttata atatgcctgc atttaaatat cacggaatgt tggtttactt
     3661 tgcagcttat aaaaatcaca ttggttttta cgctacacca acgggacact ctcaattcaa
     3721 ggaagaatta tcgatttaca aacaaggaaa aggttcggtt caattccctt tgacaaagcc
     3781 tttaccatta gatttaattt caagaatagt tcaatttagg atgaatgaaa acctaataaa
     3841 atcaactaaa aaataagcca agcaataagc taaattgttg tttaccatta aaaactttat
     3901 aaaactttaa cgtttgtttt cctgcttata gctttggatt ttacgaaatt tgcaactcaa
     3961 aaatttagaa atgatttcaa agaaaattat tgccttattg ctgttttttt taaccacaat
     4021 tgattctttt tcccaagaca caaactccag tgaactaaca aagcaaaaat tcacattgag
     4081 tggaaccatt accgatgcca atagcaatga aacgctaatt ggagtaaacg tttccatcgc
     4141 tgaattaaaa actggggtaa caaccaatga atacgggttt tattctataa cgctaccaaa
     4201 aggaaactac accttgcaaa ttgcgtattt gggttttcaa aatatggaag aaaaaattca
     4261 attatctcaa aattttaaaa gaaatttcaa actacaaagt agcgaacaac aattgcaaga
     4321 agttgtagtc acctccaaca aaaaaagcac caacatcaaa acgcccgaaa tgagtgtgaa
     4381 caaactctcg atgggaacca tcaaaaaaat gcctgtggtt ttgggagaaa ccgatgtgtt
     4441 gaaatccatt ttattgcttc caggagttac aaacgctggc gaaggacaat cgggttttaa
     4501 tgttcgtggt ggtgccgccg atcagaatct ggttttatta gacgaggcta caatattcaa
     4561 ttcctctcac gtttttggtt ttttctcggt ttttaatccc gatgcgatta aggatttaaa
     4621 actctacaaa ggtggaatcc cgtcacgtta tggcggcaga gcttcatctg tattagaaat
     4681 ttatcaaaaa gacgggaaca gcaataattt tcatatgaac ggaggaattg gtttaatctc
     4741 tagtcgatta ttagccgaag gtccgattgt aaaggacaaa ggctctttcc taattggtgg
     4801 tcgaacttcc tatgcacatt tgttcttgaa attggcagac aatgacaatt cggcttcgtt
     4861 ttatgatttg aatgccaaat taaattacaa actaaacgct aataatagcc tttatgtttc
     4921 gagttatttt ggtagagatt tttttaatat tagcaaagaa tttcaaaata cgtacggcaa
     4981 cagcactttc aatttaagat ggaatcattt gttttccgaa agacatttct ctaatttgtc
     5041 ggccattttt agtgattatt attacggttt aaccctgtct tcggtaggtt ttgattggca
     5101 atcgagtatt aaaaatttca atataaaata tgatttaaaa tactatttga acgacaaaat
     5161 caaattgagt tatggactga attctattta ttacaaattc aatccaggaa caatcgaacc
     5221 cattgatgcc aattcgagta ttaatttcaa gcaattggac aagaaaaatg cttttgaacc
     5281 ttcgatttat ttggaagctg agcaccaagc cactcaaaaa ttatcgttta tgtatggttt
     5341 gcgttacagt atgttttata gattgggagc atcaactgtt aatttatacg aaaacaatca
     5401 agctgtaacc ttcaataatc aactcaaaat ttacgaaaaa ggagtgccaa caggaacaaa
     5461 atcttacggt tataatgaag taatcacctc gttttcaaat ttagaacctc gattttctag
     5521 ttcgtatgtt ttagacgaaa aatcatctat aaaagccagt tacaaccgaa tggtgcaata
     5581 cctgcaattg gtttcaaaca ctgcctcgcc tactccatta gacatttgga caccgagcga
     5641 tcaatacatt caaccgcaaa ttgccgatca agtggctgcg ggttatttca gaaatttcaa
     5701 agaagatatg tattctttgg aagtggaaag tttttacaaa aaagtaaaaa accgaatcga
     5761 ttatattgat ggtgctgatt taattgccaa caacgccatc gaacaagtaa ttctcaacgg
     5821 acaattgaga gcttatggat tggaattgat gttgcgcaaa aacagtggta aattcaacgg
     5881 ttgggttgca tatactttat ctaattcgga acaacaaact cccggtagaa gtccaaacga
     5941 aaccggaatc aacaacggaa attggtacaa atcgggttat cataaattac acaatttggc
     6001 tgtaacggct aattacaatt tgaatccaaa atggactttc ggaggaaatt tcatcttgca
     6061 atcgggtcaa cctgtgactt ttccgaacgg acaataccaa taccaaggca ttactgttcc
     6121 tagttatggc ggtcgaaacg aaaacaacct gccattatac caccatttag atttggcggc
     6181 aacttacaca cctaaacccg agaaaagaaa aggatggcag agcgaatggg tttttagtgt
     6241 ttacaacatc tataatcgaa aaaatgccgc ttcgattagc ttccgagaaa accaaacatc
     6301 tggagctaat gaagctgtca aattatccat ttttggcgca gttccatctg tgagttataa
     6361 tttcaaattc taaaactatg caaattttaa aatatattac actatcattg ctacttcttt
     6421 ccatcatcag ttgtgaagaa gtaatcgatg ttgatttgaa aacggaaaca ccaagattgg
     6481 ttattgatgc cgcaatcaat tggcaaaaag ggacagatgg ttcatctcaa aaaataatgc
     6541 tatctaccac aactagtttt tacaatacta tcattccaaa agtttctgga gccactgttt
     6601 ctgtaaaaag tagtgaaaat tttaattata tttttaccga aatacccaac acaggcgaat
     6661 accaatgtac tactttcaaa cccatcattg gcgaaactta taccctaact gttgtctata
     6721 aaaacgaaac ctataccgcg accgaaacca tgcaatcagt cagttctatt tatagcattc
     6781 aacaaaaaaa tgacggcgga ttttcgggca aagattatga aattgaagtt tcgtttaaag
     6841 atccgctagt taaaaatttt tatatggtaa aattctttcc tgatatttat aaagcaccca
     6901 gttatcaagt tattggcgac gaatatacaa atggaaatct aaaatcttgg caattcagtg
     6961 acgaagattt aaaacaagga agtaccatcg ccataaccca ttacggagtt tctgaatcgt
     7021 attacaatta tatgaacaaa ctcatctcgg tagcaagcag ttcaggcgga ggaagtccat
     7081 ttcaaacacc accagcgact gtaagaggaa atattgttaa ccaaactaat atcaacaatt
     7141 atgctttggg ctatttttca ctaagcgaag ttgattatca aaattatatc attaaataaa
     7201 acagaaatca aactcgcaat ttattgtctt tcctctctta aaattacaaa ttttcaatta
     7261 cgaattacga acctagaaat actatctttg aaaattaatc cataattgaa aaatcataat
     7321 tcctatttaa ctatgtcttc acaccatatc gttcgcgatg accaagaacc cgcattaatc
     7381 attgccaacg gagctgcttg taatcccgaa ttattgggac aattactcga atggtcgcca
     7441 ttggtaatcg tgcttgactc ggcaatggaa cgggttttag aattgggcat aaaagtagat
     7501 gttttgttag gcgatttcga ccgtggtttt gaccctaatt actataaaga aaaacaatat
     7561 ccattggaaa ttgtccacac accagaccaa aacaaaaccg atttagaaaa agcatttgat
     7621 tacctaatcg aacgagaaat tccagccgta aacgtagttt gggccaccgg aaaacgtgcc
     7681 gatcatacta taactaatat taccaacatc agtcgttaca gagatttaat taaaatcgtt
     7741 gttttggacg accactccaa agtgttttta ttgccaaaaa aattcgaaaa atggtatcct
     7801 gccaacacac cactttcctt gattccagta ggtcacgtta ccggaattca ttctgaaaat
     7861 ttattttatc ctttagaaaa tgatacgcta accattggct atagaaccgg aagcagcaat
     7921 cacgtaaaag aagatggaat agtaattata gaacaccaag aaggagattt attaatgatg
     7981 gaatgttggg attaattgat tgtagatttt agattttcca catcaatcta aaatctgaac
     8041 tctaaaatct aaaatcctat ttttcaacta tctttgcatc gaaataaaga aaatgaaatt
     8101 gaaaccgatc atcgaaaaaa cgaatttaca tcccagaaat caacatcgaa tgggatataa
     8161 ttttgaagaa ttaattgccc tttgtcctga acttcaaccc tatgtttcca taaatgaaca
     8221 taatataaaa accattgatt tcagtaatcc tcaagctgta aaagcactca acaaagcctt
     8281 gctgattgcc aattacgaca tccaaaattg ggatattcct tcaacgaatc tttgtccacc
     8341 cattccggga cgcgtagatt atattcatta tatcgcagat ttattagcgt ccaacaataa
     8401 cggcatcatt cccgaaggcg aaaatgtgca ggttttggac attggtattg gtgccaattg
     8461 tatttatcct attttgggaa atacagttta tggttggtct ttcgttggaa ccgaaattga
     8521 cgaaaaagcc attcaaaatt gcaaaaaaat cattgaagct aatccaagat taatggatta
     8581 tattagtctg caattgcaaa ccgaatcgcg ttttattttc aaaaacatca tcacgcccga
     8641 agatcgattt tcgctaacaa tttgcaatcc accttttcat aattctgccg aagaagcgac
     8701 aaaagcttcc attcgcaagg tcaacaatct cgaaaataca cgaactacaa agcctgtctt
     8761 aaattttggt ggtcaaaatg ccgaattatg gtgtgaaggt ggcgaactgg gtttcatcac
     8821 ccaaatgatt ttcgaaagtg ccaaattccc gatgcaatgc ctttggttta cgaccttggt
     8881 ttccaaaaaa gagaatcttt ccagtttata caaaaccctc aacaaagtca atgtggtcga
     8941 agtaaaaacc atagatatgg cgcaagggca aaaaacaagt agaattttag cttggacttt
     9001 tcaaacggaa gcgcaacaga agaactggaa gtttgaactg gaatagcctc tataactagt
     9061 atttcttaac ttttacattt ttccacaaac tgtaattaat ctaaatcaaa tgcatttttc
     9121 aatcattccc tttcttttag tctcaggtta tgttgtaaca attagtagat caatgctaaa
     9181 tgaaagatat aagaaaagat taatattatc tgtggtaagt ctaattttag gtatagtatt
     9241 cgaaattttt tcaaaagaag aaaaaagatt ttcatatatt gttttcggat tattgccatt
     9301 aatgtacctt ttttattatg aaatacttcg aattattttt atgcctctaa taggtaaatt
     9361 tccatattta ccgtatcgag aaaaaattgg agcaaaagtc ttaggctatg attatccaaa
     9421 aaacagaaaa gtaaaattgg ccgattacat ttttggaatg actttaattt ttcttccatt
     9481 aataactatg ctatttttag gtatcttatt aaataattat ttcaaataac atctttttga
     9541 tattttactt tctgcttctg tttt
//
LOCUS       DAOSNN010000079         9489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7179_length_9489_cov_5.344393, whole genome shotgun sequence.
ACCESSION   DAOSNN010000079 DAOSNN010000000
VERSION     DAOSNN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7179_length_9489_cov_5.344393"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLQ70_05575"
     CDS             <1..260
                     /locus_tag="PLQ70_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQE34120.1"
                     /translation="WISQIPDKSRVFLMSSHASTSIYEAEFQDGDWLVFGKESVGLSE
                     EVLSQFDNHLTIPMSNLIRSYNIANSVAFVVGEAKRQIRLK"
     gene            265..1074
                     /locus_tag="PLQ70_05580"
     CDS             265..1074
                     /locus_tag="PLQ70_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34121.1"
                     /translation="MEGNVTVNQALNRGRWQLKYLPMIVTFGCIIGCFFLVEYTFLGG
                     FIIPIGFVLGFSMGWLVWSYYVMEWKIWAYENVRNIHELQRKAVEEKLIWETGSWFEK
                     TEFKNYQQKQKLYQLEKKFLEKDVYKDDISVPKETIIYYSRKTILLMLIVFLGLGFFG
                     VFLLFEKEYLGLLFLGIGIYFSFDQAKKLRDKNPQIVLNDKGIKLKNENLVSWNKIFN
                     DRVFSESNSKSSTNYLAFNAEKIVIDELTIEYEELENLLHVYRVRYEKYNY"
     gene            complement(1071..1853)
                     /locus_tag="PLQ70_05585"
     CDS             complement(1071..1853)
                     /locus_tag="PLQ70_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE34122.1"
                     /translation="MENKIILQTSNLSIGYTSKKEKNSIASNINLSLEKGKLIALIGA
                     NGIGKSTLLRTITGIQKPLSGTVFLNKKNIHELDSLTLAQNLSVVLTEKLPPSNLTVW
                     ELVALGRQPYTNWIGTLTENDITKINEAIALTQIEHLASKKHYEISDGQLQIVLISRA
                     LAQDTPLIILDEPTTHLDLLHKVALLKLLKKLTQETEKCILFSTHDIDMAIQLSDEMI
                     IMTPETTLQDEPCNLILKGSFNTLFKDEHIVFDSEKGKFVIS"
     gene            complement(1853..2884)
                     /locus_tag="PLQ70_05590"
     CDS             complement(1853..2884)
                     /locus_tag="PLQ70_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQE34123.1"
                     /translation="MQNKNRNTLLFSLLTLSLLLLLVLNISLGSVSIPIKDVFHSLIG
                     ENSSKETWDYIIVNYRLPKAITAVLVGMGLAISGLLMQTLFRNPLAGPDVLGLSSGAS
                     LGVATVILGAGFLPPFLASFLLSSYGIILASSLGSFLVLLAVLSVSQSLKNTMAILIV
                     GLMFGSLTNAIVGTLTYFSTAEQLQKFTFWSLGNLGNLAWSSIVILLVCIIIGLFMSL
                     LNIKSLNALLLGENYARSMGMNYKKTRLIIILATSILTGSITAFAGPIAFIGLAVPHI
                     AKLVFQTSNHTILFWSTLLLGAIILLICDSISQLPGSDVTLPINAVTSLFGAPIVIWL
                     LLRKRKMMN"
     gene            complement(3082..4221)
                     /locus_tag="PLQ70_05595"
     CDS             complement(3082..4221)
                     /locus_tag="PLQ70_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQE34124.1"
                     /translation="MKSVQNTAFYFLVFLLFIQCKENPKKEKTIATSENEIQYAEGLE
                     IYKHLGFTILKITNPWPEAKESFTYILQEKNGIIPDSLKQFPLIQIPLKSIVVTSTTH
                     IPALELLGVENTLVGFPDTNYISSEKTRKRIDTGKVRDVGKNESLNTEVLIDMNPNLI
                     VSFGLNNSNPSLDNLQKSGLKVILNGDWTEQSPLGKAEWIKFFGVLYGLDSKANTIFS
                     DIVKEYNSTLALAQKVTQKPTVLCGAMYQDQWYVPQGESWAALFLKDAQSQYLWADTK
                     GNGSLSVPFEAVLEKAQNADFWIAPGDFSSLKEMSKSNPHYNQFASFKNKKVYSYAIN
                     KGAKGGIIYFEWSPTRPDWVLKDLIKIFHPELLPNHKLFFFQKLE"
     regulatory      4484..4747
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4826..6766
                     /locus_tag="PLQ70_05600"
     CDS             4826..6766
                     /locus_tag="PLQ70_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HQE34125.1"
                     /translation="MNNKIVRISALFVLMTNCVFAQQKDSIASKTELDEVVISDSKFA
                     LAKEKSGKIITKITSEDLKMKEGQSVAAILNSVAGVEINGSQSAAGKNLGYYIRGGKS
                     NQVLILIDGIPVTDASGISFEYDLRLLPAEQVESIEIMKGASSTLYGTGAATGLINIR
                     LKKAGKKAIQGSGYWNLGTYNTASTSKTNPQDYNQGFSVNGNINKVNYFASLNSTETT
                     GMSQIAEPNENIKYEADRFSRINYLGKIGYKPTNKLNLDFFGNYDKINNDYDGGFDNT
                     GANDVIQNHSKSEQFRFGFLPKYKYNRGEFVLNSSFNKMVRSYDELDSYSGTVGLSQY
                     ESRSVNVDAYNKYEIAKSLFLVAGAQYQFHDMNSATPYGGVAKENAKFNMIDPYVTGV
                     YTSGFGLNVNAGVRWNIHSEYGNQVVYNLNPSYDFKAFPLKVLASYSTAFVTPSLYQL
                     YSPYGNTALTPEKNTTAEAGFETNLLDKKIKFSTVGFYREQTDFIGFSSAYKYINIEG
                     TNKAKGVETEITLALSQKLKWISNYTFTQVDKALDRLIPKHKVNSSLDYQIFTRTTFN
                     VNYQYVDGRNDAFFDGNTFGTQNVVLGSYQLVNASLNYELIKNRLSLFGAATNLLNEE
                     FFENVGYNTLGRNFKLGLNIKL"
     gene            complement(6836..8311)
                     /locus_tag="PLQ70_05605"
     CDS             complement(6836..8311)
                     /locus_tag="PLQ70_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQE34126.1"
                     /translation="MKTTFKLIIVFLLASFVFQSCQDQDDVAAPVSLEVNDFIWKGLN
                     LYYLWQADVPNLSDNKFANQTELNTFLNGYSKPENLFESLLYKPESLYPATEAVDRFS
                     WIVDDYLELEGQLQGTTNNNGVEFGLSRKSTGSSEIFGWVRYIIPGSDASTKSIKRGD
                     IFYGVNGTQLTINNYQALLFGSNNDYTLNMADINGGAFTPNGKSVALTKTVLDENPIL
                     INKVIVSGSHKIGYLMYNGFYSNYDSQLNDAFGTLKSQGITDLVLDLRYNGGGSIQTA
                     TRLASMITGGFTGKVFAKEQWNAKIESYFNANDPESLKNLFVDKMGTTPINSLNMTKI
                     YILTSAATASASELVINGLKPYINVVQIGDVTVGKNVGSVTLYDSPTFGKENRNPNHR
                     YAMQPLVLKIVNSVGYGDYQSGLVPTFQLKETLSTLDVLGSTSEPLLNLAVGKITGTA
                     KTKQTVPGVQFDYFKDSKSTNGIQNQMYLDKAPEGLLKALE"
     gene            8486..9013
                     /locus_tag="PLQ70_05610"
     CDS             8486..9013
                     /locus_tag="PLQ70_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HQE34127.1"
                     /translation="MKKIIVTAVLVLVSNVFFAQSVFDKYEGQDDVASIIVNKKMFQM
                     MGSVKVDANDKETQQYLDLMKKLDNLKVFSTSNTKVSSQMKVTADQYYKTAGLEELMR
                     VDNGDKNLKIWVKSGVTENQIKELLVYNAGANKENQTVLMLLTGNFTLNEIAVLIDKM
                     KIPGGVDLKNATKGK"
     gene            complement(9091..9423)
                     /locus_tag="PLQ70_05615"
     CDS             complement(9091..9423)
                     /locus_tag="PLQ70_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34128.1"
                     /translation="MQTKITLKSKINKTLFLAFSLIALTTFEVKSQTVIYDSISKQKV
                     ALIDVRKTYERIIDKGYASIEMFEYLGFYYYNDKDFQKSKLYFDMLFKKYKLSQISQK
                     SIDLYKTL"
BASE COUNT         3133 a         1459 c         1708 g         3189 t
ORIGIN      
        1 aatggattag tcaaattcca gataaatccc gtgtattttt gatgagttcc cacgcttcaa
       61 catccattta tgaagccgaa tttcaagatg gagattggtt agtttttggt aaagaaagcg
      121 ttggtttaag cgaagaagtt ttgtctcaat tcgataatca tttgactatt ccaatgtcga
      181 atttaattcg aagttataat attgcaaatt cggtagcttt tgtggttggc gaagcgaaaa
      241 gacagattag attgaagtag ttttatggaa ggaaatgtaa ctgttaatca ggctttgaat
      301 aggggaagat ggcaattgaa atatttgccg atgattgtta cttttggttg tattattggt
      361 tgtttttttc tcgtagaata tactttttta ggaggtttta ttatacctat tggatttgtt
      421 ttaggttttt caatgggttg gcttgtgtgg agctattatg ttatggaatg gaaaatttgg
      481 gcttatgaga atgttagaaa tattcacgaa ttacaaagaa aagcagtcga agaaaaactg
      541 atttgggaaa ctggaagctg gtttgaaaaa acagaattta aaaactacca gcaaaagcaa
      601 aagttatatc aattggagaa aaaatttctt gaaaaagatg tttataaaga tgatatttca
      661 gtgcctaaag aaactataat ttattattca agaaagacaa ttttattaat gctaattgta
      721 tttttgggtc ttggtttttt tggagttttt cttttatttg aaaaagaata tttgggattg
      781 ttgtttttag gaattggcat atacttttca tttgaccaag caaaaaaatt aagagataaa
      841 aatccgcaaa ttgttctgaa tgataaagga attaaattga aaaatgaaaa tctagtttct
      901 tggaataaga tttttaatga tcgcgttttt agtgaaagta acagtaaaag ttctactaat
      961 tatttagcat ttaatgctga aaaaattgta attgatgagt tgacaattga atatgaggaa
     1021 ttagaaaact tactccacgt ttatagagtt cggtacgaaa aatataatta ttaagaaatc
     1081 acaaacttcc ctttttcgct atcaaaaaca atatgttcat ctttaaacaa tgtgttgaaa
     1141 cttcctttta atattaaatt acacggttcg tcttgtaggg tggtttcagg agtcataata
     1201 atcatttcat cactcaattg tatcgccata tcgatatcgt gagtcgaaaa cagaatacat
     1261 ttttccgttt cttgggttag ttttttcaat agttttagga gcgctacttt gtgtaataaa
     1321 tccaagtgtg tggtgggttc gtccaaaatg attaagggtg tatcttgcgc caaagctctt
     1381 gaaattaata cgatttgcaa ttgtccatca ctaatttcgt aatgtttttt ggatgctaaa
     1441 tgctcgattt gagtcagtgc aatcgcttcg ttaattttgg taatgtcgtt ctcggttagt
     1501 gttccaatcc aattagtata aggctgacga cctaaagcta caagctccca aactgtgaga
     1561 ttgcttggtg gtaatttttc ggttagaact acacttaaat tttgtgccaa agtcaaggaa
     1621 tctaattcgt ggatgttctt tttattcaaa aaaacagttc ctgaaagcgg tttttgtatt
     1681 cctgtaatgg ttctcaacaa agtcgatttt cctataccgt ttgccccaat taaagcaatc
     1741 agttttcctt tttctaaaga caaattaatg tttgaagcaa tgctgttttt ctcttttttg
     1801 gaagtatagc caatgcttaa attggaagtt tgtaagatga ttttgttttc cattaattca
     1861 tcattttacg ttttcgtagc aatagccaaa tcacgatggg tgcaccaaat agtgaagtca
     1921 ctgcgttaat aggcaaagta acatcacttc caggaagttg cgaaatgctg tcgcaaatca
     1981 gcaggataat ggctcccaaa agcaaggtgc tccagaataa aatagtatga ttgctggttt
     2041 gaaaaaccaa tttagcaatg tgcggcaccg ccaatccaat aaaagcaatt ggccctgcaa
     2101 aagccgtaat gcttccagtg agaatactgg tggcaagaat aataatcaag cgagtttttt
     2161 tgtagttcat tcccatactt cgggcgtagt tttcgccaag caacaaagca ttcagggatt
     2221 taatattcaa aaggctcata aataaaccaa ttataataca aaccaataaa attacaatcg
     2281 acgaccaagc taaattaccc agattaccca acgaccaaaa agtaaatttc tgcaattgtt
     2341 cggcggtact gaaataggtt aatgttccca caatagcatt ggtcaagctc ccaaacatca
     2401 atccgacaat tagaattgcc attgtgtttt tcaaactttg cgaaaccgat aaaacagcca
     2461 ataaaaccaa gaaacttccc aaacttgaag ctaagataat tccataagac gaaagtaaga
     2521 acgaagctaa aaaaggaggc aaaaaacctg ctcccaaaat aaccgttgcc acacccaaac
     2581 ttgctccaga actcaatcct aaaacatcgg gtccagccaa tggatttcgg aataaggttt
     2641 gcatcaataa tccgctaatc gctaatccca ttccgactaa tacagccgtg atggctttgg
     2701 gtaatcgata gttaacgatg atgtaatccc aagtttcttt gctggaattt tctccaatca
     2761 aactatgaaa aacatctttt attggaatag aaaccgaacc caagctgatg ttcaatacca
     2821 atagcaatag caatgacaat gtcaaaaggg agaataaaag ggtatttcgg tttttgtttt
     2881 gcaagtttga tttgtttatt gtgccgtctg ttcgagtgtt atattaaaaa atgcgatagc
     2941 tttttttaat aaaatgtatc gagaacttaa ctattatagg cttctcgata caatttttaa
     3001 cattaaagct gttgcattaa tgttaaaaat cactcgaagt gacgagcgga atttgttatt
     3061 tttctattat attaattatt attattccaa tttctgaaag aaaaacaatt tatgatttgg
     3121 caataattca ggatgaaaaa ttttaatcaa atctttcaaa acccagtcgg ggcgagtagg
     3181 ggaccattcg aaataaatga tacctccttt tgcacctttg tttatcgcat acgaatatac
     3241 ttttttattt ttgaacgaag caaattgatt gtaatgtgga ttgcttttgc tcatttcttt
     3301 caagcttgaa aaatctccgg gtgcaatcca gaaatcagcg ttttgagctt tttctaaaac
     3361 ggcttcaaac ggtacagata aacttccgtt tcctttggtg tctgcccata aatactgtga
     3421 ttgagcatct ttcaaaaaca aggctgccca actttcgcct tgcggaacat accattgatc
     3481 ttgatacatt gcgccacaca aaactgttgg tttttgagtc actttttgag ctaaagccaa
     3541 tgtcgaattg tattctttta caatgtcaga aaaaatggta tttgctttag aatctaagcc
     3601 atataagact ccgaagaact taatccattc ggcttttcct aacggagatt gttcagtcca
     3661 atcgccgttg agtatcactt ttagtccgct tttttgtaga ttgtccaaag agggattgct
     3721 attgttcaaa ccaaaactca caatcaaatt gggattcata tctattaaaa cttcggtatt
     3781 taggctttcg tttttaccca catctcgaac ttttccggtg tcaattcgtt ttcgtgtttt
     3841 ttcggatgaa atgtaattcg tatcaggaaa acctactaaa gtattttcta cgcctaataa
     3901 ttccaatgct ggaatgtggg ttgttgaggt caccacaatc gattttagcg gaatttgaat
     3961 tagcggaaat tgcttcaaac tatcgggaat aattccgttt ttttcttgca gaatataggt
     4021 aaaactttct ttggcttctg gccaaggatt ggtgattttt agtattgtaa atcctaggtg
     4081 tttatagatt tccagtcctt cggcatactg aatttcattt tctgaagtag caattgtctt
     4141 ttcctttttt ggattttctt tgcattgaat gaacaacaaa aagactaaaa agtaaaacgc
     4201 agtattttga acggatttca tttaatgaat tttttacaaa agtattgttt tgttggcaca
     4261 aaaataaact tgtgtaactt ttgatacatt tttggcaatg caaagaaagt aattttgcta
     4321 gaaactatct atgagagaca attcaaacac ttttatgtat gtcgtggcag gaataatagt
     4381 cttgcatttt ttagttggat ttatttggtt gattgtcaag ctttcaaaaa agaaagagaa
     4441 cgaagaggat tgattttatt tttttattca aaacttagaa ctacatttgc cgccgtattg
     4501 aggttgctgt tttatttttt tgaaaccgca ttaaaaggga atcaggttta aaacctgggc
     4561 tgtacccgca actgtaagct aaaaagcttg ttgttatcta caaaaccact gtttggaagt
     4621 gggaggttag aaggtagagg ttagaagtta aatacttctt acttcgtatt tctaatttct
     4681 aacttagaat gggaaggtaa acaacaagac gcaagccagg agacctgcct attacatcga
     4741 gtatcaaact ttcgggaaaa aaggtttggg tatgggtcat tctatgcttt tctcccattt
     4801 ttattattaa aaatgtttta ttaaaatgaa caacaaaatc gttcgtatta gtgcgttatt
     4861 cgtactaatg actaactgtg tttttgcgca acaaaaagac agtattgcat ccaaaactga
     4921 attggatgaa gtagtaattt cggattcaaa atttgctttg gcaaaggaaa aatcaggaaa
     4981 aataattact aaaatcactt ccgaagattt aaaaatgaag gaaggacaaa gtgtagctgc
     5041 cattctaaat tcggttgctg gtgtcgaaat caacggaagc caaagtgctg ccgggaaaaa
     5101 tttgggttac tacattcgtg gtggaaaaag taatcaagtc ctgattctta ttgacggaat
     5161 tccggttact gatgcttcgg gaattagttt tgaatatgac ttgagattac ttcctgcgga
     5221 gcaagtggaa agtatcgaaa ttatgaaagg cgcttcgagt actttgtatg gaacgggtgc
     5281 tgcgacaggt ttaatcaata tccgtttgaa aaaagcaggc aaaaaagcca ttcaaggaag
     5341 tggctattgg aatttgggaa cctataacac ggcttctact tcaaaaacta atccacagga
     5401 ttacaatcaa gggttttcgg taaacggaaa catcaataaa gtgaattatt ttgcttcatt
     5461 gaatagtaca gagactactg gaatgtcgca aattgctgag ccaaatgaaa acatcaaata
     5521 tgaagccgat cgcttttcga gaatcaatta tttggggaaa attggctata aaccaaccaa
     5581 taaattgaac ttggatttct ttggaaacta cgacaaaatc aacaatgatt atgacggagg
     5641 ctttgacaat acaggagcaa acgatgtaat tcaaaatcat tcaaaatcag aacaatttcg
     5701 ttttggattc ttgccaaaat acaaatacaa cagaggtgaa tttgttttga attcgagttt
     5761 taacaaaatg gtacgttctt acgatgaatt ggattcttat tcaggaacgg ttggcttgtc
     5821 tcaatacgaa tccaggagcg tgaatgtgga tgcctacaat aaatacgaga ttgcaaaatc
     5881 attgtttttg gttgctggag cacaatacca atttcacgat atgaatagtg caacgcctta
     5941 tggtggagtc gccaaagaaa atgccaaatt caatatgatt gacccttatg ttacgggagt
     6001 ttatacttcg ggttttggat tgaatgtcaa tgctggagta cgatggaaca ttcatagcga
     6061 atatggaaat caagtggttt ataatctgaa tccttcttat gattttaaag catttccgtt
     6121 aaaagttttg gcttcttata gcacggcatt cgtgacgcca agtttgtacc aattgtattc
     6181 tccttatgga aatacggctt tgactccaga aaaaaacact actgccgaag ctggtttcga
     6241 aacgaatctt ctagataaaa aaatcaaatt ttctaccgta gggttttatc gagaacaaac
     6301 tgattttatt ggattttcat cggcttataa atatatcaat attgaaggaa cgaataaagc
     6361 aaaaggtgtt gaaacagaaa ttactttggc tttaagccaa aaattaaaat ggatttccaa
     6421 ttatactttt acccaagtgg ataaagcttt ggacagattg attccgaagc ataaagtgaa
     6481 ttcgtctttg gattatcaaa tttttacaag aactactttt aatgtcaatt atcagtatgt
     6541 tgatggtaga aatgatgctt tcttcgatgg aaatactttc ggtactcaaa acgtagtttt
     6601 gggttcttac caattggtaa atgcctcttt gaattatgaa ttaattaaaa acagattgag
     6661 tctttttggt gcggctacta atcttttaaa tgaagaattt tttgaaaatg tagggtacaa
     6721 tactttgggt agaaacttta aattagggtt aaatattaag ttgtaatttt aagatttaac
     6781 aaccataaaa aaggctttca gtgatgaaag ccttttttgc atttatgttt ttattttatt
     6841 ccaaagcttt caatagacct tcgggagctt tgtctaaata catttgattt tgaattccgt
     6901 ttgtagattt tgaatctttg aaataatcaa attggacacc aggaacggtt tgttttgttt
     6961 ttgcagttcc agtgattttg ccaacagcaa gatttaacaa aggttctgaa gtactgccaa
     7021 gaacatcaag agtgcttagt gtttctttta attgaaaggt tggaactaaa ccactttgat
     7081 agtctccata acctactgaa ttgacaattt tcagcaccaa aggttgcatc gcataacggt
     7141 gatttggatt tctattttct tttccaaaag ttggcgaatc atataaagta actgagccta
     7201 catttttacc cacagtaaca tcgcctattt gaaccacatt aatataaggt ttcaaaccgt
     7261 taattaccaa ctcacttgcc gaagcagttg ctgccgaagt cagaatataa attttggtca
     7321 tatttagact attaataggt gtagttccca ttttgtcaac aaaaagattt tttagggatt
     7381 cagggtcatt ggcattaaaa taggattcta ttttagcatt ccactgttct ttggcaaaaa
     7441 ctttacctgt aaatccgcct gtaatcatac tggccaatct cgttgcagtt tgaatagaac
     7501 cgccgccgtt gtatcttaaa tcaagaacta aatcggtaat tccttgtgat tttaatgttc
     7561 caaaagcatc atttagttga ctgtcataat ttgaataaaa accattgtac atcaaataac
     7621 caattttatg tgaaccagaa acaataactt tattaattag tattggattt tcgtccaaaa
     7681 cagtttttgt caacgccact gattttccgt ttggagtaaa agctccacca ttgatgtcgg
     7741 ccatatttaa agtatagtcg ttgttggaac caaagagtaa tgcttggtaa ttgtttatag
     7801 taagttgagt tccatttaca ccataaaaaa tatcaccacg tttgatgctt tttgtagagg
     7861 catccgaacc aggtataata tagcgtaccc aaccaaatat ttctgatgaa cctgtagatt
     7921 ttcggcttag gccaaattct actccgttat tattagtagt tccttgaagt tgtccttcta
     7981 attctaaata atcatctaca atccagctaa aacgatcaac cgcttctgta gcaggataaa
     8041 ggctttcggg tttgtagagt aaactttcga ataaattttc gggtttagaa tatccattca
     8101 aaaaagtgtt taattctgtt tgattggcaa atttgttgtc ggataaatta ggcacatcgg
     8161 cttgccataa atagtacaaa ttcagtcctt tccatataaa atcgttaact tctaagctaa
     8221 caggtgcagc tacatcgtct tggtcttgac aactttgaaa aacaaatgaa gctaaaagaa
     8281 atacaattat aagtttaaaa gttgttttca tattttgaat ttgataggtc taacgtaaaa
     8341 gtacgaattt aaaattttaa cattatcttt attaacaaaa gtcattccaa cttaataata
     8401 aaatcataaa atttagaatt ttattgcatt ggcacggcct ttgttattat tttgctatat
     8461 ttataaaata gtataacaca aaaaaatgaa aaaaattata gtaacagcag tattagtttt
     8521 ggtttcgaat gtgtttttcg cacaatcggt ttttgataaa tatgaaggtc aggatgatgt
     8581 ggcatcgatt atcgtcaaca aaaaaatgtt tcaaatgatg ggaagcgtaa aagtggatgc
     8641 caatgacaaa gaaactcagc aatatcttga cttgatgaag aaattagaca atcttaaagt
     8701 tttttctaca tcgaatacta aagtttcgtc ccaaatgaaa gtaactgcag atcaatatta
     8761 taaaactgct ggtttagaag aattgatgcg agttgataat ggtgataaaa atcttaaaat
     8821 ttgggtaaaa tctggcgtaa ccgaaaatca aatcaaagaa cttttagttt ataatgctgg
     8881 tgctaacaag gaaaatcaaa cggttttaat gttgttgacg ggtaatttta ctttgaatga
     8941 aattgcggtt ttaattgata aaatgaaaat tcccggtgga gtcgatttaa aaaatgcaac
     9001 taagggaaaa taatcttaat atatcattga gtaaaaaaaa gagcaacccc aattgaaagt
     9061 tgctcttttt ttgttcaaat tattcaaagt ctagagtgtt ttgtataaat caatgctttt
     9121 ttgtgatatt tgagataatt tatatttttt gaataacata tcgaagtaca acttcgattt
     9181 ttgaaaatct ttgtcattat aataataaaa acctaaatat tcaaacattt caatagaagc
     9241 gtatccttta tcaataatcc tttcgtaagt tttacgtaca tcaattaatg ctactttttg
     9301 tttgctaata gaatcgtata taacggtttg agatttgact tcgaaagttg tcaatgcaat
     9361 caatgaaaat gccaaaaaca atgttttatt tatttttgat ttcaatgtaa ttttggtttg
     9421 cattaataca attttattta aagaggacag ctaaattaca tttatataat caatatgcaa
     9481 atttcagag
//
LOCUS       DAOSNN010000080         9440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7232_length_9440_cov_4.434949, whole genome shotgun sequence.
ACCESSION   DAOSNN010000080 DAOSNN010000000
VERSION     DAOSNN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7232_length_9440_cov_4.434949"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLQ70_05620"
     CDS             <1..587
                     /locus_tag="PLQ70_05620"
                     /inference="COORDINATES: protein motif:HMM:NF039702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6250 domain-containing protein"
                     /protein_id="HQE34129.1"
                     /translation="FDKDWSKWDVEFEKPETSSLKIIDGQLDVSTTIGATIWFKTQLS
                     GNIMITYDVKIIDEGGSFDRVSDMNAFWMATDPSSSNTIKRDGKFASYDDLNLYYAGV
                     GGHYNTFTRFRKYKGIDDKSILKEYSDKEHLLVGNKTYSVKIMVNKGRIQYFLDNKLY
                     WDFQDEKPYQKGFFGFRTSKSHQQFDNFKVFEIE"
     gene            complement(601..1269)
                     /locus_tag="PLQ70_05625"
     CDS             complement(601..1269)
                     /locus_tag="PLQ70_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQE34130.1"
                     /translation="MKIGLTFSESNYANYPRWIKETDSTIEIIELSYEVNNWQDVALC
                     DAIIFTGGIDMDPIEKVAYANAPKEFNWVRDRFEMAVLAKALDKKKPILGICRGLQLI
                     NVYWGGSLHLDNGDAKNKIHRRESEDKIHRIQVEKDSLFYSIVQQEFGNVNSAHHQSI
                     DVLGLGLKAVAHSEDGVIEVIESANPDEQFLLAVQWHPERMADLESPFSKNILRALLE
                     KIGY"
     gene            1436..3541
                     /locus_tag="PLQ70_05630"
     CDS             1436..3541
                     /locus_tag="PLQ70_05630"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQE34131.1"
                     /translation="MKKFRDLNTYFKLLLLIISTSVLFFLLYVALYIYTNKQEHHVYK
                     STQEQYDNEVSSIIQLNSKTLVSTLVDITFWDELVKFTKTKDKKWYDVFIASQFESYE
                     ADYLGVYGIDNQFITKTSNTKIKTIDFIPKEVLTRLYEAKLTRFYMKIPEGVVEVFGA
                     TIHPSDDPKKNKSKPSGYFFMVRLLDQNFCKNLDNISSSNVRLVALNNTTNIQKDVLE
                     SDVKLLDYKNTAIANLVFKRPFNLHYQNTKEILIILILVSLINVFIYLYFSRKWIYKP
                     LKTITDVLETGNKMAIKDLQKEPGEFGYIGYLFEENSNQRKQLELSKEKAEESDKLKS
                     SFLANLSHEIRTPMNAIVGFSDLLNEPNLSEDSKSEYLKIIKNCGINLVSIIEDLIEM
                     SKIDSKQIVPNISSVDLDACIAELYETLKVTIPVDKDLELYFVKNESPTSGNILTDVI
                     KLKEVIVNLISNAIKYTEKGQIIFSYQINEKEKTIVFAVKDTGLGIDEENLKVIFDRF
                     RRIEDEFSAQLSGLGLGLAISKAYVEMLGGKISVSSQIKQGSVFTFTIPLVYDETSPI
                     LNQNTSLKRGNKSNYETILVAEDDNINFLLMKKILQLKKYKVLRAENGQEAVDMCREN
                     PDIHLVFLDIKMPLLNGFEAFKIINSFNPKLPVIAQTAYASAEDFDKIMKHGFIAYIS
                     KPLDKEKIYELIDSVFHNS"
     gene            complement(3683..4366)
                     /locus_tag="PLQ70_05635"
     CDS             complement(3683..4366)
                     /locus_tag="PLQ70_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HQE34132.1"
                     /translation="MKTHTKESQDQITPRRALEILQEGNDRFIKNLKAHRDLLEQANE
                     TRDGQWPFATILSCIDSRTSAELIFDQGLGDIFSVRIAGNVINTDILGSMEFACKLAG
                     SKLIVVLGHSKCGAVKGACDHVEMGNLTELLSKIQPSVYEEKTTTSDRNSKNDLFVQN
                     VATINVKRSVKNIIERSFILEQMVENGEIGIVGAWHDIETGKVSFYDDVMYIKDDKNP
                     KFTVADLRH"
     gene            complement(4392..5987)
                     /locus_tag="PLQ70_05640"
     CDS             complement(4392..5987)
                     /locus_tag="PLQ70_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQE34133.1"
                     /translation="MSKKINLFANLKSDFASGLVVFLVALPLCLGIAMASGAPLFSGI
                     ISGVIGGIVVGYLSTSHISISGPAAGLTAIVLTAITDLGAFDIFLTAVFIAGLIQLAL
                     GFLKAGSLSNYIPTNVIEGMLAGIGIIIILKQLPHAFGYDSDFEGDLAFSQTDGSNTL
                     TSLLGIVDYVQLGSIVITAISLIILIAWDKVPFLQKLKLIPAALIAVTIGVVLNEIFT
                     ATGSSLTIAKEHLVSLPIPTSIEEFKQIIVTPNFSGITNPKVWVVGLTIAIVASIETL
                     LCIEAADRMDVHKRYTDTNVELKAQGIGNIVSSLLGGLPMTSVVVRSSANANAGAQSK
                     MSSIIHGSLLLLSVLTIPALLNKIPLATLAAILLLVGYKLAKPATFKHFWKNGKYQFA
                     PFIATLLAVVFLDLLKGVALGLLISVFAILRGNMKRAYIFRKEEYHDGDVIHIDLAQE
                     VSFLNKAAIKTTLSKIPENSKVVINAQDTVYIAHDVLDLIKEFKKIRAKEENIKVKLT
                     GFKKEYELENTGEEEKHVFVTQS"
     gene            complement(6077..6358)
                     /locus_tag="PLQ70_05645"
     CDS             complement(6077..6358)
                     /locus_tag="PLQ70_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34134.1"
                     /translation="MKLIARLLLILFVAFLITPTVVCVIKKDADISTFYSFSEEEKAQ
                     KEIKAIINLDLNCTTVDVSPFNCKIILSENTSKHDTISSKIFIPPPDKA"
     gene            7047..8057
                     /locus_tag="PLQ70_05650"
     CDS             7047..8057
                     /locus_tag="PLQ70_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQE34135.1"
                     /translation="MKKVTLILALMLTSTLTFAQDAEAPKSTPLEISGSGDLYYKYDF
                     AKQSGNIPTSFAGDHNSVSLGMLDIALKKTVGKASFVGEISFGPRGQYESIPNGSDGN
                     SFHIQNLYASYAVSDKLSLTAGYMGTFIGYEVISPVANFHYSTSYLFTNGPFQNAGVK
                     ANYKVSDKFGVMLGAFNDQWNVYTSDFGIKAIGAQLSYASGPVSAYLNFMDGKNSGTI
                     FDLTATLQLAEKFKLGINAADYSNDGDAGYTGFALYPSVALTDSFGLGLRAEYFQYKE
                     GSGDTDVTAFTLSGNYKVGGLTIIPEFRLDSNSDKVIFEDSDSDPSKSASQFLIAVVY
                     GF"
     gene            complement(8349..9263)
                     /locus_tag="PLQ70_05655"
     CDS             complement(8349..9263)
                     /locus_tag="PLQ70_05655"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQE34136.1"
                     /translation="MKQITLNTFYLLTLAIIITSCSSNPYKASNKSYKEQLHDYKKTI
                     SNTEAAKLETSSTTILPNTEAVIDPLSLYKDTLSIKTPVALNNGISTEWISTVNFNLR
                     KPNFIIIHHTAQDSLAQTLKTFTTTKPQVSAHYVVADDGRVVQMVNDYLRAWHGGNAI
                     WGKNTDINSASIGIELDNNGTEPFSDKQIISLLALLTKLKKDYNIPTQNILGHSDIAP
                     SRKVDPSKYFPWKLLASNGFGIWPDEILEPAPANFNAELALRIIGYNTKNLAAAISAF
                     KLHYIQTEVDSVLDEKTINTIYNIYKKQ"
BASE COUNT         3092 a         1370 c         1608 g         3370 t
ORIGIN      
        1 atttcgataa agattggtcg aaatgggatg tcgaattcga aaaaccagaa acgtcaagcc
       61 taaaaataat tgatggtcaa ttggatgttt ccaccactat tggagcgact atttggttca
      121 aaacccaatt gtcgggaaat atcatgatta cttatgacgt gaaaattatt gacgaaggcg
      181 gtagttttga ccgagtttct gatatgaatg ccttttggat ggcaaccgat ccttcaagtt
      241 caaatactat aaaaagggac ggaaaatttg cgtcttatga tgatttgaat ttgtattatg
      301 ctggtgttgg tgggcattac aacaccttta cccgattccg aaaatacaag ggaattgatg
      361 ataaatccat cttaaaagaa tatagcgaca aggaacatct tttggtcgga aacaaaacct
      421 attctgttaa aataatggtc aataaaggtc gcattcaata tttcttagac aataaattat
      481 attgggattt tcaagatgag aagccttatc agaaaggttt ttttggattt aggacttcaa
      541 aaagtcatca gcaatttgat aattttaagg tttttgaaat agagtaaaat ttaattcaaa
      601 ttagtaacct attttttcta gtaaagccct taggatattt ttgctaaaag gactttctaa
      661 atcggccatt ctttcgggat gccattgcac agccaataga aattgttcgt ctggatttgc
      721 tgattctata acttcgataa cgccatcttc agaatgagcg acagctttta gacccaaacc
      781 taatacatcg atagattgat gatgcgcact attgacatta ccaaattctt gttgtacaat
      841 cgaataaaat aaggagtctt tttctacttg aatacgatga attttatctt cagattctct
      901 tctatgaatt ttatttttgg catcgccgtt atctaaatgc aaactgccac cccaataaac
      961 gttgattaat tgcaaacctc gacaaatgcc aagaataggc ttttttttgt ctaaagcttt
     1021 tgccaaaaca gccatttcaa atcgatcacg aacccaatta aattcttttg gagcgttagc
     1081 atatgctact ttttcaatag gatccatatc aattccgcct gtaaaaatga tggcatcaca
     1141 aagagctaca tcttgccaat tatttacttc atacgaaagt tcaataattt caatagtaga
     1201 atcggtttct tttatccacc tcggataatt ggcataatta gattcagaaa aggttagtcc
     1261 aatcttcatt ttactagatt agttcgtaaa gataaaaatc ttcagtctaa tagaagaggt
     1321 ttatgtgttg aattctaatt taataggatg ttttttttat tattttttaa ctcaaattac
     1381 taaattaatt tatctttata actaatttta ttttttgtga attttattca attctatgaa
     1441 aaagtttcga gacctcaata cctacttcaa gctactttta ttgattataa gcactagtgt
     1501 tttgtttttt ttgttatacg ttgcgcttta tatttatacc aataaacaag aacatcatgt
     1561 ttataaatct actcaagaac agtatgataa cgaagtttcc tcaattattc agctcaattc
     1621 aaaaacactt gtatcgactc ttgttgatat aacattttgg gatgaattag taaagtttac
     1681 taaaactaaa gacaagaaat ggtatgatgt ttttatagca agccaatttg aatcctatga
     1741 agcagactat cttggggtgt atggaataga taatcagttc attactaaaa cgtctaatac
     1801 taaaattaaa actattgatt ttattcctaa agaggttttg actcgattgt atgaggcaaa
     1861 attgactcga ttttatatga aaattcccga gggtgtggtt gaagtttttg gagccacaat
     1921 acatccatca gatgatccca aaaaaaataa gtcaaagcct tcaggctatt tttttatggt
     1981 aagactttta gatcagaatt tttgtaaaaa tttggacaac attagtagtt cgaatgtgag
     2041 attagttgcc ctaaataata caaccaacat tcagaaggat gttcttgaat cggatgttaa
     2101 actattagat tacaagaata cagcgattgc aaatttagtt tttaaaagac cttttaattt
     2161 gcattatcag aacacaaaag aaattcttat tattttgatt ttggtttctt tgattaacgt
     2221 ttttatttat ttgtattttt ctagaaaatg gatttataag ccgttgaaaa ccataacaga
     2281 tgttctagaa acaggcaata aaatggcaat taaagatttg caaaaagagc ccggagagtt
     2341 tggatatatt ggctatttat ttgaagaaaa cagcaatcaa cgaaaacagt tagagctctc
     2401 aaaagaaaaa gctgaagaaa gtgacaaact taaatcttct tttttggcca atttatctca
     2461 tgaaattcga accccaatga atgctatagt aggattcagc gatttgttga acgaacccaa
     2521 tttgagtgaa gattctaaga gtgaatattt gaaaattata aaaaattgtg gaattaattt
     2581 ggtgtcaatt attgaagacc ttatcgagat gtctaagatt gattccaaac aaattgtgcc
     2641 aaacattagt agtgttgatt tggatgcttg tatagccgaa ttatacgaaa cattaaaagt
     2701 tactattcct gtggataaag atttggaatt gtattttgtt aaaaatgagt ctcctacctc
     2761 tggtaatatt ttaaccgatg tcataaaact caaagaggtt attgtcaatt tgatttcaaa
     2821 tgcaataaaa tataccgaaa aaggacaaat tatttttagc tatcaaataa atgaaaagga
     2881 gaagactatt gtatttgcag ttaaagatac aggattagga atagacgaag aaaacttgaa
     2941 agtaatattt gacaggttta gaagaattga agatgaattt tcggctcaat taagtggttt
     3001 aggtctaggt ttggccattt caaaagctta tgttgaaatg cttggaggaa aaattagtgt
     3061 tagttcacaa atcaaacaag gctctgtttt tacatttaca ataccattag tttatgatga
     3121 aacaagtccg attctgaatc aaaatactag cctaaaaaga ggtaacaaat caaattatga
     3181 aaccattttg gttgccgaag acgacaacat caactttttg ttgatgaaga aaatattgca
     3241 attaaaaaaa tataaagttc ttcgagccga aaatgggcaa gaagctgtag atatgtgtcg
     3301 agaaaatcct gatattcatt tggttttttt ggatataaaa atgcctttat tgaatggttt
     3361 tgaggctttt aaaattataa attcttttaa tccaaaactc cctgttattg cacaaactgc
     3421 ctatgcatcg gctgaagact ttgataaaat tatgaaacat ggattcatag cttatatttc
     3481 taaacctttg gataaagaaa aaatatatga attaatcgat tctgtttttc acaatagttg
     3541 atggttgtga gttttgtttt aatgctgatt gaaaatataa aaacagtgta aatataaaat
     3601 ggttgccttt tgagacaacc atttttttga tatgcaaatt ttaggatcta atattgttca
     3661 atattagaat cagtaagagt tgttagtgcc tcaaatcagc tacggtaaat ttcggatttt
     3721 tatcatcttt gatgtacatt acgtcgtcat aaaaagatac ttttccagtt tcgatatcgt
     3781 gccaagcgcc tacaattcca atttctccat tttcaaccat ttgttccaaa ataaaactgc
     3841 gttcaataat atttttcaca gaacgtttta cattgatagt agctacattt tgaacaaaaa
     3901 gatcgttttt tgaatttctg tcagatgtag ttgtcttttc ttcataaact gaaggttgga
     3961 tttttgataa taattcagta agatttccca tttcaacatg gtcgcatgcg ccttttactg
     4021 ccccacattt gctgtgacct aaaactacaa tcagtttgga accagctagt ttacaagcaa
     4081 attccatact gcctaaaata tcagtattga taacatttcc agctattcta actgagaaaa
     4141 tatcgcctaa accttggtca aaaattaatt ctgccgaggt tcgactatca atacaactaa
     4201 gtatggtggc gaatggccat tgaccatcac gggtttcatt ggcttgttct aataaatctc
     4261 tatgagcttt taagttcttg ataaatctat cattaccttc ttgtaaaatt tctaaagctc
     4321 ttcttggtgt tatttggtct tgtgattctt ttgtatgtgt tttcatttgt cgagagttaa
     4381 attaatgttt tttaagattg ggttacaaat acgtgttttt cttcttcgcc tgtattctct
     4441 aattcatatt cttttttaaa tccagttaat tttactttaa tattttcttc ttttgctcta
     4501 atttttttga attctttaat taaatccaat acatcgtgag ctatataaac agtgtcttga
     4561 gcattaatta ctaccttaga gttttcggga attttactta gagttgtttt gattgcagct
     4621 ttgtttaaga atgaaacttc ttgggctaaa tcgatatgaa ttacatctcc gtcgtgatat
     4681 tcttcttttc tgaaaatgta ggctcttttc atgtttcctc tcaaaatggc aaaaacacta
     4741 atgagtaatc ctagagcgac tccttttaat aaatctaaaa atacaacagc taataaggta
     4801 gcgataaagg gtgcaaattg gtattttccg tttttccaaa aatgtttgaa tgttgctggt
     4861 tttgccaatt tgtatcctac tagtaataag atagcagcca atgttgccaa cggaattttg
     4921 ttcaaaagtg caggaattgt caagacactt aacagcaaaa gagaaccatg aatgatggaa
     4981 gacattttag attgggctcc agcattggca tttgccgaag agcgaaccac tacagatgtc
     5041 attggcagac cacctaaaag ggagctaacg atgtttccaa taccttgagc tttcaattct
     5101 acattagtat cggtgtagcg tttgtgaacg tccattctat cagctgcttc gatgcataat
     5161 aaagtttcaa ttgaggctac tattgcaata gttaaaccta caacccaaac tttaggattt
     5221 gtgattccag aaaaattggg agttacaata atttgtttga actcttctat tgatgttggg
     5281 atgggtaacg aaactaaatg ttcttttgca atggttagtg aactaccggt tgctgtaaaa
     5341 atttcattca atacaacccc tattgtgaca gctataagtg ctgcgggaat taattttaat
     5401 ttttgaagaa aaggaacttt gtcccaagct attaaaatga ttagggaaat ggcagttatg
     5461 acaatggatc ctaattgaac ataatcgaca atgcctaata gagaggtcaa agtgttgcta
     5521 ccgtctgttt gagaaaaggc taaatctcct tcaaaatcac tgtcgtaacc aaaagcatgg
     5581 ggtagttgct ttagaataat tataattcca atacctgcca acattccttc aataacattt
     5641 gttggaatat aatttgatag gctaccagct tttaaaaaac cgagtgctaa ttgaattaaa
     5701 ccggctataa atacggctgt caaaaatata tcgaaggctc ctaaatcagt aattgcggtc
     5761 aatacaattg ctgttaaacc agccgctgga ccagaaatgc taatgtgaga ggtgctcaaa
     5821 tagcccacaa ctattcctcc aataacacca gaaataatac ctgaaaataa aggcgctccc
     5881 gaggccattg ctattcccag acataatggg agtgctacta aaaaaaccac taaacctgag
     5941 gcaaaatcag atttaaggtt tgcaaaaaga tttatttttt ttgacataat accttactta
     6001 aaattaaacg tgaaagattt gccatctcaa taatgagtat agcaactgat tttaattcat
     6061 taaaatcaat gaaaaattaa gctttgtcgg gtggggggat aaatatctta gaggagatag
     6121 tatcgtgctt ggaggtattc tctgaaagta ttattttaca attaaacgga gatacatcta
     6181 cagtagtaca atttaaatca aggttgataa ttgctttaat ttctttttgc gctttttctt
     6241 cttcagaaaa gctataaaat gtagaaatat cagcatcttt cttaattaca caaactacag
     6301 ttggagtaat tagaaaggct acaaataaaa tcaataatag tctcgcaatt aatttcatag
     6361 tttgcaaaaa tagaatttag gaaatcatat caaagctaat agtataaatt taaaataaaa
     6421 ttaacatctg ctaattgtcg ttggaaaatt atttagaaaa tgctgctttt tttattcaat
     6481 aaataacagt gaaaaaaggt tttttttacg gttgttaaat ggtactcagt ttgtttgttt
     6541 attttgcttt tcaatggtta aaaaagatgg atttttctct taaatggatt taattttaaa
     6601 aattaatttc cattttggct gatttatgat tttttaataa aatttagaca tgggtatttt
     6661 tttttagggg taatctttgt tatttattca taaaagctat ttttttttga attttattgt
     6721 caatttttgc ttaaatgatg caaaaaatag ggggtatgat taaaaataaa aatagtaatc
     6781 tgataaaata aattgattta aatagggatt gtatattttt taatattcat tttttggtta
     6841 ttgacaattc tttcaagaat aagtcaaaaa agagttttta tactgtttta gtttgacaaa
     6901 agttgataga tttgtcacag aaaaatggtg tttttcaaac cgaatttaga cctactttaa
     6961 tgatgtattt cttttttgaa aacacatttg gtactaataa attcaagatt aaaaaaaact
     7021 aatttaactt ttaaactaaa acaaacatga aaaaagtaac cttaatttta gctttaatgc
     7081 taacgagcac tttaacattt gcacaggacg cggaagctcc aaaatcaact ccattagaaa
     7141 tttcaggttc tggtgatttg tattataagt atgattttgc taaacaatca ggaaatattc
     7201 caactagttt tgcaggtgat cataattcag tttctttggg tatgcttgat atagctttga
     7261 aaaaaacagt aggaaaagca tcttttgttg gtgaaatatc tttcggacca agaggacaat
     7321 acgaatcaat tcctaatgga tcagatggaa attcattcca cattcaaaat ttatatgcat
     7381 cttatgctgt aagtgataaa ttgagtttga ctgctggata tatggggact tttattggtt
     7441 atgaagtaat ttcgccagta gctaatttcc attattctac ttcttatttg tttacaaacg
     7501 gaccattcca aaatgcaggt gttaaagcaa attataaagt atctgataaa tttggagtta
     7561 tgttaggggc gtttaacgat cagtggaatg tatatacatc tgattttggt attaaagcta
     7621 ttggagctca attgtcttat gctagtggtc cagttagtgc ttaccttaac tttatggatg
     7681 gtaaaaacag cggaacaatt tttgatttaa ctgcaacttt gcaacttgct gaaaaattca
     7741 aattaggtat caatgctgct gattattcaa atgatggtga tgcaggttat acaggatttg
     7801 ctttgtatcc atctgtagct ttaacagatt cttttggttt aggattgcgt gctgaatatt
     7861 ttcaatacaa agaaggttca ggtgatactg atgtaactgc ttttactttg tcagggaact
     7921 acaaagttgg tggattgact attattccag aatttagatt ggatagtaat tcagacaaag
     7981 taatctttga agattcagat tcagatcctt caaaatctgc ttctcaattc cttatagctg
     8041 ttgtttacgg attctaattt ttgaagtaaa aaacgatttt aaaaatattt ttttttactg
     8101 ccaagatgcc gtcatagtat cttggcagtt tttttgtaat agagatagtc gaaactgcta
     8161 gtttatcaaa ccgatttggg ttgtctatag tatatttgat gtacagacta ttttgatgtt
     8221 tgttccaatc acttttataa gataatttaa ttagttcgat tgaatttgtc agtgctagca
     8281 agtcgtttgt ctctgcaata agatcaaata aaaaagcggg ctctgaagta attcaaagcc
     8341 cgctttaatt attgtttttt gtaaatattg tatattgtat taattgtttt ttcgtctaaa
     8401 acactatcaa cttctgtttg aatgtaatga agtttgaaag ctgaaattgc tgctgctaaa
     8461 ttttttgtat tgtaaccaat tattcttaat gctaattcag cattgaaatt agctggtgct
     8521 ggttctaaaa tttcatctgg ccaaatacca aatccatttg aagctagaag tttccaaggg
     8581 aaatacttac taggatcaac ttttcggctt ggcgcaatat cagaatgacc taatatgttt
     8641 tgagttggaa tattgtaatc ctttttgagt tttgtaagca atgccaaaag gctaataatt
     8701 tgtttgtctg aaaacggttc agttccatta ttgtctaatt caattccgat ggatgctgaa
     8761 ttgatatcgg tatttttccc ccaaatggca tttcctccat gccaagcgcg aagataatcg
     8821 ttcaccattt gaaccactct tccgtcatcg gcaacaacat aatgggcgct tacttgaggt
     8881 tttgtggttg taaaggtctt taatgtttga gccaaagaat cttgtgcagt atgatggata
     8941 ataatgaaat taggttttct tagattaaaa tttacggtgc ttatccattc tgtactaatg
     9001 ccgttgttca atgcaacagg agtttttatc gatagtgtat ctttgtaaag agacaaaggg
     9061 tctataactg cttcagtatt gggtagaatt gtagtgctgc tagtttctaa tttggccgct
     9121 tctgtgttag aaatagtttt tttataatcg tgaagctgtt ctttgtatga tttgttacta
     9181 gctttataag ggttagaaga gcaagaagta ataattattg ctagagtcag aagatagaaa
     9241 gtatttagtg ttatttgttt cattccgttt gtttgtctat tggttttgaa atgtattatt
     9301 tctttttatc ttttgatttt gattttttgg gttgtttttg ttcttcttta aaagcgagat
     9361 atttttcttt ttgatcttta ttcaaaaaac cgttgatttt tctatcggtg gtttccgtta
     9421 gagctttata ttcatttatt
//
LOCUS       DAOSNN010000081         9437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7235_length_9437_cov_5.715519, whole genome shotgun sequence.
ACCESSION   DAOSNN010000081 DAOSNN010000000
VERSION     DAOSNN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7235_length_9437_cov_5.715519"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="PLQ70_05660"
     CDS             <1..1527
                     /locus_tag="PLQ70_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34137.1"
                     /translation="TKWEEKQDVTISAVAIAGTDMGKKWGGNQDYISNLKIKNKSEAT
                     ITHIMDSISYDINLKWKGTASIKEKAKHYFVVEPSKSNTIELSCSFIQTDKNKSEIPS
                     FEAIEKSSIAGWESFWESGAAVDFSGSTDPRAHELERRVVLSQYLTKVQCTGSMPPQE
                     TGLTYNSWYGKPHLEMHWWHGVHFALWGRPELLEKSMPWYQNAFDKAKKLAERQGFKG
                     ARWQKMTDPDGNESPSSVGALLIWQQPHFITYAELLYRNSPSPATLEKYKERVFATAD
                     FMASFAHYDAEKKRYFLGPGVIPAQERFKAMETFNPTYELAYWNWALNTALAWKKRLN
                     EPAPKEWQDVLDKLSPLPVAGNVYLATESATDSYTNPEFKTDHPSVLGTFGMLPETTL
                     LDKKIMKNTFDLVWNTWSWDKTWGWDFPMTAMSAARLNMPEKAIDALFMNVVTNTYLK
                     NGHNYQSERLTLYLPGNGGILTTVAMMCAGWDGSTGNNPGFPKDGSWNVKWEGFKPMP
                     "
     gene            1752..2417
                     /locus_tag="PLQ70_05665"
     CDS             1752..2417
                     /locus_tag="PLQ70_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34138.1"
                     /translation="MEKSIFEKHPSKLLNELYSKKNYQGANPADAKILFVGRDPNWAF
                     DVESKEMFNYVSDYLTDGVTFWKTHDIHHPFLLPNYKGDGKRYHKIFSKLNVESSLAS
                     KISFVELIGFPTTGMAKTNNKFFLNYLISTENRKHLIELDNLLNDFEKKIFVAWGLLE
                     DFKFLNRETGLFQKLANLDKSKMVISDLNQHGNIYIHRHFSDAISNATLEKMKIEIKR
                     NVD"
     gene            complement(2501..3265)
                     /locus_tag="PLQ70_05670"
     CDS             complement(2501..3265)
                     /locus_tag="PLQ70_05670"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019975717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HQE34139.1"
                     /translation="MKKRMIGIMGAMPEEIEGVVGLLSNCEVRSMGQRSYFTGQINGI
                     DAVVVFSRWGKVAAATTVTTLIHEFKITELLFTGVAGAISLDLKIGDIVLGKRLIQHD
                     MDARPLMKQFEIPLLSKTYLESDATHLATAQKAIEIVFENKRLHTIFTEEDLKAFSIS
                     EPRLFMGDIASGDQFFSSNEQKNNLNAQLPAVLCVEMEGAAVAQVCYEYEIPFSIIRT
                     ISDGADAQSHIDFPSFIKKISSKYAAEIIKNIFEKL"
     gene            3352..3846
                     /locus_tag="PLQ70_05675"
     CDS             3352..3846
                     /locus_tag="PLQ70_05675"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HQE34140.1"
                     /translation="MIKYSFPPISNPKAKILILGTMPGAQSLALSQYYGHQRNAFWKI
                     MFSLFDRPFSMDYEQRKSLLLENHIAVWDVLEACIREGSLDSAIEQEVPNDFNSFLKT
                     HPNIKHIFFNGQKAAAYFKQYIKLDKDYSLTTLPSTSPANAGKSFESKLSDWSVIKIR
                     LMKF"
     gene            3997..5553
                     /gene="pelA"
                     /locus_tag="PLQ70_05680"
     CDS             3997..5553
                     /gene="pelA"
                     /locus_tag="PLQ70_05680"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HQE34141.1"
                     /translation="MNKLKIIIAFVGFLFLSACSSDKDAVDTQVKSIAINNATAITDG
                     LAKQLSTTILPSTAANKAVTWTVSDATIAVISETGVLTPIKNGTVTVTATAKDGSGIS
                     KQVTITITGVAVPVILATSVTVGGVNSTDGQTQQLTLDVQPANATNKTVTWSTNSGIA
                     TVSATGLLTPKSNGTVIIYATANDGSGKVGQLTLTISNIAFTTILKAENMLLWQRNNG
                     GWPKEPYNDFTGYDRAQTSTEVTTANNTKGNTDTTIDNNHTVGELRYLLAAYKSSKNT
                     NYLTAAEKGIDFLFTAQYANGGWPQYYPDKSGYRHQITYNDNAMGNVMNLMWDISKSL
                     KDTEVVNPKYKAMAVTAFEKGIDVILKTQITSPAGKKTAWCAQHDEVTLLPATARAYE
                     LPSVSGSESVGITRILMMVESPSPAVKQAVKDAVDWFNSAKLYDIKTQATTNPTDVIV
                     VASPGAILWARFYDLNTGLPFFCGRDGIKKATLAEIEVERRTGYSWYGNWPSTLISTE
                     YTNWKAKNGL"
     gene            5687..6631
                     /locus_tag="PLQ70_05685"
     CDS             5687..6631
                     /locus_tag="PLQ70_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34142.1"
                     /translation="MISISPKTKQSLLFIIKIVIVGGAFYFIYNQLAHNDKLDWQKFL
                     LLFQKNYSITGIAFLLLLSVLNRFFEILKWQNLVSYLHKISLAEAAKQVLGALTAGLF
                     TPNGIGEYAGKALFFEKSKAKTIVFLNLICNGIQMILSVFFGTFGLLYLNADYNIITS
                     KTVLILFGICLFLFGILFFLKKINIKGYSIEKLIHKINEIPKSIHQKNIFLAICRYLV
                     FSHQYYFLFLAFDVNLPYFTLMATVAAVYFLASSLPTFQFLDFAVKGSVAVYFFGILG
                     VNEWIVVFISTLMWFLNVVLPVVIGSYYVMNFKIKTEQ"
     gene            6628..7728
                     /locus_tag="PLQ70_05690"
     CDS             6628..7728
                     /locus_tag="PLQ70_05690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE34143.1"
                     /translation="MIWALYTILFIYCLTILTLIYGFTKVNSFEYLGLEPKTKFSIVV
                     PFRNEAENLPKLLDSLSKLEYPTDLFEVILVDDFSSEAFQVSSFKFQVLVVNNIRVSN
                     SPKKDAISTAMKIAKNDWIITTDADCVVNENWLSALDNYIQLHKVSMIAGAVTYDCEN
                     IFLHHFQQLDLASLQGATMGSFGIRKGFMCNGANFAYTKSFFQDLNRFDGNDGIASGD
                     DVFLLQKGIAQFPEKVHYLKSENNIVITKPVDNWKSLFHQRVRWASKTGSYQSRFGIG
                     LGLIVFGGNAGWLLVVGCWLLGKDNFYVLISLLLAKMTVDFVLINKTNQFLKNKTLYY
                     FISSLLYPFFSVSVALYSLFGKYEWKGRRFSK"
     gene            complement(7721..8431)
                     /locus_tag="PLQ70_05695"
     CDS             complement(7721..8431)
                     /locus_tag="PLQ70_05695"
                     /inference="COORDINATES: protein motif:HMM:NF025175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HQE34144.1"
                     /translation="MPFTFSHPAIVLPLLRNKNLSATGLIIGSMSPDFEYFIRMKVES
                     NISHTFIGLLFFDLPIGFLVALLFHQIIKRNLIENLPPFFQNKLATLKNTKWVDYLKM
                     NLFYVLISILIGTISHILWDAFTHESGWFVQKMPFLSHQINNISLYKILQHLSSLIGM
                     LFIFYYVHKLPVEKNTILKQYWKYWTLVFVLTFVFMSIRLGFGLSFNQIGNVVVSLIS
                     SIILAMTFAGNFKNNEVI"
     gene            complement(8435..8917)
                     /locus_tag="PLQ70_05700"
     CDS             complement(8435..8917)
                     /locus_tag="PLQ70_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34145.1"
                     /translation="MRKFILILFFLSQLFSSCQYFDKQVPSEKELLQKELKAINWKEV
                     DEFPSVAGCEKIENKTLRQQCFFEYMTQLIQQRLSVDTLSILYPELDTIEVKVTVYPN
                     SRIIFEPLFPKDSVAYDKIKIDSILKARLVGFPKVNPAIKRGIPVKTQFILPVILKVE
                     "
     gene            8998..>9437
                     /locus_tag="PLQ70_05705"
     CDS             8998..>9437
                     /locus_tag="PLQ70_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HQE34146.1"
                     /translation="MDLLLLIIGFVLMVLGVFGSFLPVLPGPSLSWIGLVLLYFTNAV
                     PVNYWILAITFLITVLISVLDYVIPAKGTKKFGGSSYGIWGTNIGLIVGIFAPIPFGF
                     IIGPFVGAFIGELIYDYKDHGRAFKAATGSFIGFLAGSFMKFVI"
BASE COUNT         3108 a         1592 c         1687 g         3050 t
ORIGIN      
        1 acaaaatggg aagaaaaaca ggatgtaact atttcagcag ttgcaattgc ggggactgat
       61 atgggaaaaa aatggggcgg aaatcaagat tatataagca atctgaaaat caaaaataaa
      121 tcagaagcta caattacaca cataatggac agtatttcgt atgatattaa tttgaaatgg
      181 aaaggtactg catcgattaa agaaaaagca aaacattatt ttgttgtaga accttcaaaa
      241 agtaatacaa tcgaattgag ctgttcgttc attcaaacag ataaaaacaa atctgaaatt
      301 ccttcttttg aagcgattga aaaaagtagt atcgctggtt gggaatcctt ttgggaaagt
      361 ggtgcagcgg ttgacttttc aggaagtact gacccaagag cacacgaatt ggaacgaaga
      421 gttgttcttt cgcaatacct taccaaggtt caatgtacag gttcaatgcc tccacaagaa
      481 acaggtttaa cgtacaacag ttggtacgga aaaccacact tggaaatgca ttggtggcac
      541 ggggttcatt ttgcactttg gggaagacct gaattgttgg aaaaaagtat gccttggtac
      601 caaaatgctt ttgataaagc caaaaaacta gccgaaagac aaggttttaa aggagcaaga
      661 tggcaaaaaa tgaccgatcc tgatgggaat gaaagtcctt cttcggtagg tgcacttttg
      721 atttggcaac agccgcattt tatcacttat gccgaattgt tgtatcgcaa ttctcctagt
      781 ccagcaacat tagaaaaata caaagaacgt gtttttgcca ccgccgattt tatggcttcg
      841 ttcgctcatt atgatgcaga aaaaaagaga tactttttag gccctggtgt gattccagca
      901 caagagcgtt tcaaagcaat ggaaactttt aatccaactt atgaattggc gtattggaat
      961 tgggctttga acacggcatt ggcttggaag aaaagattga acgaacctgc tccaaaagaa
     1021 tggcaagatg ttttggataa actatcgcca ttacctgttg ctggcaacgt gtatttggct
     1081 accgaaagtg ctaccgattc ttatactaat cctgaattca aaaccgatca cccatccgtt
     1141 ttgggaactt ttggaatgtt gccagaaaca acacttttgg acaaaaaaat catgaaaaac
     1201 actttcgatt tagtttggaa cacttggtct tgggacaaaa cttggggctg ggattttccg
     1261 atgacagcaa tgagtgccgc tcgtttaaat atgcctgaaa aagccatcga tgctttgttt
     1321 atgaatgtag tgaccaatac ttatctaaaa aacggacaca attaccaatc cgaaagactg
     1381 actttgtact tgccaggaaa tggcggaatt ttgaccacag tggcaatgat gtgcgctggt
     1441 tgggatggaa gtacaggaaa taatccagga tttccaaaag atggcagttg gaatgtaaaa
     1501 tgggaaggtt ttaaaccaat gccgtaaatc agatttattt tagtttttaa aaacgggttg
     1561 gaataaaaat tctaacccgt ttttttgtaa aaatctattg gaatatgaac ctaaagaatt
     1621 tctgtgcgaa agtgaattat ttatatatta gcgtaaataa agtttagcga ttgtcagact
     1681 tatactagtt agcggtaatt ttcgtaaacc gttgtaaata ggcactctga atgaaataac
     1741 gacattttaa aatggaaaaa tctatattcg aaaagcatcc gtcaaaattg ctaaacgaac
     1801 tttattcaaa aaaaaattat caaggcgcaa atcccgcgga tgcaaaaatt ctatttgtcg
     1861 gaagagatcc aaattgggca tttgatgtgg aaagcaaaga aatgtttaat tacgtttctg
     1921 attatttaac tgacggagta actttttgga aaacgcatga tattcatcat ccatttttgt
     1981 tgccaaatta taaaggagac ggaaaacgtt accataaaat tttctcgaaa ctaaacgtgg
     2041 aaagtagttt ggcaagcaaa atctcatttg ttgaactcat tggatttccg actactggaa
     2101 tggcaaaaac gaacaacaaa ttttttttaa attatttaat ttccacagaa aacagaaaac
     2161 atttaataga attagacaat ttattaaatg actttgaaaa aaagatattc gtagcttggg
     2221 gtttacttga agattttaaa ttcttaaata gagaaactgg attattccaa aaattagcga
     2281 atttggacaa aagtaaaatg gtcataagtg acttaaatca acacggaaac atctacattc
     2341 atagacattt ttctgatgca attagtaatg cgacacttga aaaaatgaaa atagaaatta
     2401 aacggaatgt agattaaaac tacccttgct cgtacccata aattctctaa agacaaaaca
     2461 ttcatatttt aaatatcttt tcatttatgc ttttaatatt ctacaacttc tcaaaaatat
     2521 tctttattat ttcagcagca tatttactgg aaatcttttt tataaaggaa ggaaaatcaa
     2581 tatgcgattg cgcatctgcc ccatccgaaa tggttcttat aatactaaaa ggaatttcat
     2641 attcataaca aacctgagca accgccgcac cttccatttc aacacacaaa acggctggca
     2701 attgggcatt caaattgttt ttttgttcgt tactcgaaaa aaactgatct ccgctggcaa
     2761 tatcacccat aaataatcga ggttccgaaa tagaaaatgc cttcaaatct tcttcagtaa
     2821 aaatagtgtg caacctttta ttctcaaata cgatttctat tgctttttgg gctgttgcca
     2881 aatgggtcgc atcgctttct aaatacgttt tagaaagcaa aggaatttca aattgtttca
     2941 tcagcggtct ggcatccata tcgtgttgaa tcaacctttt gcctaagaca atatccccaa
     3001 ttttcaaatc tagactaatg gctccggcaa ctcctgtaaa caacaattcg gttattttaa
     3061 attcgtgaat caaggttgta accgtagttg ccgctgccac ttttccccat ctcgaaaaca
     3121 ccaccacggc atctattccg ttgatttgtc ccgtaaaata agagcgttgt cccatcgatc
     3181 taacttcaca attactgagc aaacccacta ctccttctat ttcttcgggc attgccccca
     3241 ttatgcctat cattcttttt ttcatactaa agctcttgac tttttgattt agactagagt
     3301 aaatgtaagt atttttaatg ctatatttga aatactatta caatctgttt gatgataaaa
     3361 tattcctttc cacctatttc aaatccaaag gcaaaaattc taatattggg aacaatgcct
     3421 ggtgcgcaat cattggcttt aagccaatat tatggccatc aaagaaatgc tttttggaaa
     3481 attatgttca gcctttttga cagacctttt tctatggatt atgagcagcg gaaaagtctt
     3541 ttattagaaa atcatatcgc cgtttgggat gttttagaag cctgtattcg agaaggaagt
     3601 ttggatagtg ccattgaaca ggaagtgccc aacgatttta attcgttttt gaaaacacat
     3661 cccaacatca aacacatctt tttcaacgga caaaaagcgg ctgcttattt caaacaatac
     3721 ataaaactgg acaaagacta ttctttgacc actttaccct caacaagtcc cgccaatgca
     3781 ggaaaatctt ttgaatcgaa attaagcgat tggagtgtta ttaaaatacg gttgatgaaa
     3841 ttctagttta tccctttttt attgatttgt caaaatacta atctaaaatt ttggcattac
     3901 aaaattttat ctgcttaaaa atgtgtaatc gattgcaatt ttattatttt tgtgattcga
     3961 ctaataaaaa tatttcaatt attaacccct atttcaatga ataaacttaa aataattata
     4021 gcctttgttg gctttctatt tctatcagct tgcagtagcg ataaagatgc tgttgacact
     4081 caagtaaaat ctattgcaat taacaatgcg acagccatta cagatggact tgccaaacaa
     4141 ttatcgacca ccatacttcc aagcactgct gcaaacaaag ctgttacttg gacagtttcg
     4201 gatgccacga tagcagtaat ttcagaaacg ggggttttga ctcccataaa aaatggaact
     4261 gtaaccgtaa ctgccacagc aaaagatggt tctggcattt cgaaacaagt taccataact
     4321 attacaggag tagcagtacc agttattctt gccacaagtg taacagtggg tggcgtaaat
     4381 agcaccgatg gtcaaacaca gcaacttact ttagacgtgc aacctgctaa tgctactaac
     4441 aaaacagtta cttggtcaac taattcaggc attgcaacgg tttctgcaac aggacttttg
     4501 actccaaaat ctaacggaac agttatcatt tatgctacag caaatgatgg tagcggaaaa
     4561 gtaggacaac ttacccttac catttctaac atcgctttta ccaccatcct aaaagcagaa
     4621 aatatgttgt tatggcaaag aaacaatgga ggttggccta aagaacctta caatgatttt
     4681 acaggatacg accgtgcgca gaccagtaca gaagtgacaa ccgccaataa caccaaaggc
     4741 aataccgaca ctactattga caacaatcac accgttggag aattaagata tttgcttgcc
     4801 gcctataaat catctaaaaa caccaattat ttgacggcag ccgaaaaagg aattgatttc
     4861 ttgttcacag cacaatatgc caatggtgga tggccacaat attatccaga caaaagcgga
     4921 taccgacacc aaatcacata caatgacaat gccatgggta atgtgatgaa cctaatgtgg
     4981 gacatttcta aaagcctaaa agatacagaa gtagttaatc ctaaatacaa agcaatggca
     5041 gttactgctt tcgagaaagg aattgatgtt attttgaaaa cacaaattac ttcacctgcg
     5101 ggtaaaaaaa cagcttggtg cgcccaacac gacgaagtaa ctttgttgcc cgcaacagca
     5161 agagcttatg aattgccttc tgttagcggt tctgaatcag taggaattac aagaatattg
     5221 atgatggtag aaagtccgtc acctgcagta aaacaagctg taaaagacgc tgtagattgg
     5281 tttaacagtg ctaaacttta tgacattaaa acccaagcta ctacaaaccc aacggatgtt
     5341 attgtagttg cctctcctgg tgcaatttta tgggctcgtt tttatgattt gaatacaggt
     5401 ttgccgtttt tctgtggaag agatggaatc aaaaaagcca cactagccga aatcgaagtc
     5461 gaaagaagaa caggatattc ttggtatggc aactggcctt caactttaat ttctacagaa
     5521 tatactaatt ggaaagccaa aaatggattg taatatctaa agttatccat aatagaaaca
     5581 gctcgaagca taccgtttcg ggctgttttt tttaggtcaa atggataaaa aatcattcaa
     5641 aacaaattca acgaattatc acggattaat tcgttatttt gccaaaatga tttcaatttc
     5701 tccaaaaaca aaacagtccc ttttattcat catcaaaatt gtgattgttg gtggtgcatt
     5761 ttattttatt tacaatcagc tcgctcataa cgacaagctc gattggcaaa aattcctcct
     5821 tttgttccag aaaaattatt cgattactgg aattgccttc ctcctacttt taagtgtttt
     5881 gaatcgtttt ttcgaaattt tgaaatggca gaatttggtt tcctatcttc acaaaatatc
     5941 tttagcagaa gccgccaaac aagttttagg agcattaaca gcagggttat tcactccaaa
     6001 cggaattggg gaatacgcag gaaaagcatt gtttttcgaa aaatcgaaag ccaaaacaat
     6061 tgtctttcta aacctgattt gcaacggaat ccaaatgatt ttgtcggtgt ttttcggaac
     6121 atttgggttg ttgtatttga atgcagatta caacatcatt acttcaaaaa cagttttaat
     6181 tctatttgga atttgccttt ttctttttgg aattttattt tttttgaaga aaataaacat
     6241 taaaggctat tctatcgaaa agttgattca caaaatcaat gaaattccaa aatccattca
     6301 tcagaaaaat attttcttgg caatttgtcg ttacttggtt ttttcgcacc aatattattt
     6361 tttgttcttg gcttttgatg tcaatttgcc ttattttact ttgatggcaa ccgttgcggc
     6421 cgtttacttt ttagcttctt ctttgcctac tttccaattc ttggactttg ctgtaaaagg
     6481 aagtgttgcc gtttatttct tcggaattct gggtgtcaac gaatggattg tagttttcat
     6541 cagcaccttg atgtggtttt tgaacgtggt tttgcccgtg gttattggca gttattatgt
     6601 gatgaatttc aaaattaaaa ctgaacaatg atttgggctt tatatacaat cctattcatt
     6661 tattgcttga caatactgac tttgatttat ggttttacca aagtaaattc ctttgaatat
     6721 cttggattag aacccaaaac caaattctcg attgtggttc ctttcagaaa tgaagctgaa
     6781 aatttaccaa agcttttaga tagtctttca aaattagagt atcctacaga tttgtttgaa
     6841 gtaattctgg tggatgattt ttccagtgag gcgtttcaag tttcaagttt caagtttcaa
     6901 gttttggttg taaataacat tcgggtttct aattctccga agaaagatgc tatttcaaca
     6961 gcaatgaaaa tagcaaaaaa tgattggatt atcactaccg atgcggattg tgtcgttaat
     7021 gaaaactggt tgtcggcatt agataattat attcaattac acaaagtttc gatgattgct
     7081 ggagcagtta cttatgattg cgaaaatatc tttttacatc attttcaaca attggatttg
     7141 gccagtttgc aaggtgcaac aatgggaagt tttggaatcc gaaaaggatt tatgtgcaat
     7201 ggagctaatt ttgcctacac caaatctttt tttcaagact taaataggtt tgatggaaat
     7261 gacggaattg ccagtggtga cgatgttttt ttgttgcaaa aaggaattgc ccaatttccg
     7321 gaaaaagtac attatttaaa atctgaaaac aatatcgtca tcacaaaacc ggttgacaac
     7381 tggaaatcct tgtttcatca aagagtgcgt tgggcttcaa aaacgggttc gtatcaaagt
     7441 cgttttggaa ttggtttagg attgattgtt tttggaggaa atgcaggttg gttattggtt
     7501 gttggttgtt ggttgttggg aaaagacaac ttttatgtat tgatttcttt actgttagcg
     7561 aaaatgactg ttgattttgt tttaataaat aaaacaaacc aattcctgaa aaacaaaaca
     7621 ctttattatt ttataagtag tttgctgtat ccttttttta gtgtaagtgt agctttgtat
     7681 tctttgtttg gcaagtatga atggaaagga aggcggtttt ctaaataact tcattgttct
     7741 tgaaattacc agcaaaagtc attgctaaaa ttattgaaga aattagactt accactacat
     7801 ttccaatttg gttaaacgac aatccaaaac ccaatcgaat cgacataaaa acaaaagtca
     7861 aaacgaaaac cagagtccaa tatttccaat attgctttaa tattgtattt ttttcaactg
     7921 gaagtttatg gacataatag aaaataaaca gcattcctat taatgaactt aaatgctgta
     7981 atattttata taaagaaata ttgtttattt gatgggataa aaaaggcatt ttttgtacaa
     8041 accatcctga ttcgtgagtg aatgcatccc ataaaatatg agaaatcgtc ccaattaaaa
     8101 ttgaaattaa aacataaaat aaattcattt ttaaataatc tacccatttc gtgttcttca
     8161 atgttgccaa tttattttga aaaaaaggag ggagattttc tattaaattc ctttttatta
     8221 tttgatgaaa caacaaagca actaaaaatc cgatgggaag atcaaaaaat agtaaaccaa
     8281 taaatgtgtg actgatattg ctttcaactt tcattctaat aaaatattca aaatcaggag
     8341 acattgaacc tattatcaat ccagttgcgg aaagattttt gtttcttaaa agaggtaaaa
     8401 caattgctgg atgcgaaaat gtaaatggca ttaattactc tacttttagg ataaccggaa
     8461 gaataaattg agtttttaca gggattcctc ttttaattgc gggatttact tttggaaatc
     8521 caaccaaacg tgctttgaga atactatcaa tttttatttt atcataagcc acagaatctt
     8581 ttggaaataa gggttcaaat ataattcttg agttaggata aacagttact tttacttcta
     8641 ttgtgtccag ctctggataa agaatcgata gggtatcaac actcaatctt tgctgtatta
     8701 gttgagtcat atactcgaaa aagcattgtt ggcgcagggt tttattttct attttttcac
     8761 atccagcgac tgatggaaat tcatcgactt ctttccagtt gatggctttc aattcctttt
     8821 gcaacaattc cttttctgaa ggcacttgtt tgtcgaagta ctgacaggaa ctaaaaagtt
     8881 gtgacaaaaa aaataatatt agaataaatt tcctcatatt cgttaacaaa acaaaagctg
     8941 ctttaatttt ttttaaaagt actgctttaa ttttcaaaaa tactaaaaaa atattttatg
     9001 gatttattac ttctaataat tggttttgtg ttaatggttt taggtgtttt tggaagcttt
     9061 ctaccggttt tacctggacc aagtttgagt tggattggtt tggttttgct gtatttcacc
     9121 aatgcagttc cagttaatta ttggattctt gcaattacgt ttttgattac tgttttaata
     9181 tcagtactgg attatgtgat tcctgccaaa ggaaccaaaa aatttggagg aagctcctat
     9241 ggcatttggg gaaccaatat cggattgatt gtgggaattt ttgcgcccat tcctttcggt
     9301 tttattattg gcccttttgt tggtgctttt atcggcgagc tcatttatga ttataaagat
     9361 cacggccgag cttttaaagc agctacaggt tcttttattg ggtttctggc tggcagtttt
     9421 atgaagtttg tgatttg
//
LOCUS       DAOSNN010000082         9318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7345_length_9318_cov_4.115621, whole genome shotgun sequence.
ACCESSION   DAOSNN010000082 DAOSNN010000000
VERSION     DAOSNN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7345_length_9318_cov_4.115621"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="PLQ70_05710"
     CDS             <1..1922
                     /locus_tag="PLQ70_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQE34147.1"
                     /translation="KGLTFANNSKNDKIKNFINNNLGNVYCFEKKQYEKGIEFYKKSL
                     EYSQKAADTSQIVFTKLNIAWAYFDIDQYENGLPYLEYINKYQAKFSDKSLIIILNML
                     NGMYSGHKGDFAKGENYFLTAINLGNKLGNKGDLSFSHQEYSKFLFKKGEYKKAYENL
                     VIYNKISDEIYNDDLLKKANVAGINLELDEYKREIDKVETEIELQSQSLHKSRVIVIL
                     FIIVFLVLLLLLVSLYKNFNFKKKINHDLTLANEQLIIAKDKAIEASQAKTQFLSTIS
                     HELRTPLYGVIGITNLLLDEHKELAKSPHLGSLKFSARYLLSLVNDILQINKIEENKI
                     ILESLAFNVSDEIHLVKNALSFIAQSHKNIISVKIDPAIPEYLIGDKLRLAQILMNLV
                     SNALKFTNNGEVILSANLVKVEDNLHHIEFQVSDNGVGIDAADQDRIFEKFVQVGRND
                     NDYQGTGLGLAIVKRLLGLFNSQITLESQVGIGTTFIFTIAFEHDSNKTNEIINNIKV
                     DLSSGQLFKVLVVEDNKINQTITKRIIEKNNCSCFIVDDGLQALDVLEKDVFDIILMD
                     INMPLISGFETTRKIRSKGIHTPIIALTAFAKDEITEEAIEAGMNDILIKPFESLKLF
                     QVMNEQIKKTKTAVY"
     gene            complement(1964..2431)
                     /locus_tag="PLQ70_05715"
     CDS             complement(1964..2431)
                     /locus_tag="PLQ70_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQE34148.1"
                     /translation="MKTLVKKDGMFPSLESKSIHNFFDDFITKDLFDWTDRNFAAIGS
                     NLPSVNLKETDTEIEVELAAPGMKREDFKVEVDNDMLMISSEKEEKKEEVSKKENFIR
                     REFNYQSFSRTFSLPETIDENKIEANYKDGILHVVVGKKEGAKKRTLKSIPIQ"
     gene            complement(2634..3887)
                     /locus_tag="PLQ70_05720"
     CDS             complement(2634..3887)
                     /locus_tag="PLQ70_05720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQE34149.1"
                     /translation="MLFEDLSLSKSIQKAVFEEGYTNPTPIQEQAIPLVLAGKDLIGC
                     AQTGTGKTAAFAIPIIHQLHRIVGSSKKAKEIRALIVTPTRELAVQIGQSFDTYGKYT
                     NLTQLTIFGGVSQVPQVDTLRKGVDILIATPGRLLDLHKQGFLDFDSLHTLVLDEADQ
                     MLDMGFINDVKKIVKLTPKNRQTLLFSATMPIAIRELAEMFLKDPEKVEVSPVSSTAE
                     NVEQHIYFVEKGEKRNLLYHLIKNQNLSDVLVFSRTKHGADNVVKALRKKDIPAEAIH
                     GDKSQNARQRVLDAFKNKEVGVLVATDIAARGIDIDQLPYVINFDLPNIPETYVHRIG
                     RTGRAGNEGIAISFCSKDEQPYWKDIQKLIKVEVKTISDHPYPWHSGSPETAPATAQK
                     NSNRSGGAHKSRKSNASKQNKKRWY"
     gene            complement(4154..5194)
                     /locus_tag="PLQ70_05725"
     CDS             complement(4154..5194)
                     /locus_tag="PLQ70_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HQE34150.1"
                     /translation="MEKLILFTINDLAKVTNHRSGEIKFGEKMLTVPKGIDPAQFIKT
                     CEAKYVLLGIPEDIGVRANYGRPGAASAWQCAISSIANIQHNRFCKGSQILVLGQLNV
                     CEEMKEVKDLDFNDIDDRSKLSQLVEKIDKDVSHIIFNIIKAGKIPIVIGGGHNNSYG
                     NIKGTALAKGKPINAVNFDAHSDFRILEGRHSGNGFSYAYEEGFLKKYFIFGLHENYT
                     SKSVLDIIKKIEDRVRYTTYDSINIRREKNFNQELILASQFVQTDFYGIEVDLDAIPN
                     VASSAMTLSGFSIEELRQFITYFGKHKNAAYLHICEGAPELCYEKNNHLIGKLIGYLV
                     TDFIKSNNSIEP"
     gene            5292..6173
                     /locus_tag="PLQ70_05730"
     CDS             5292..6173
                     /locus_tag="PLQ70_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34151.1"
                     /translation="MEDQQNNSNSSLKIIIGVLALLLVGSLYYIYKISTEAEQVQTVL
                     TTTVSEKESVMKDLQELKATYDAAIAENTSMSEELVKERDKVVKLMNDIDKSKGDVSK
                     YKSEVSKLQNNMKALMAENDVLKKQNKTLTIQRDSTVVVLGEAKKYNEVLVGQNEDLS
                     KTVEKGSKLTVLNMQTAAYKIRSSGKQIATDKAGRADVLKISFTIAENQIAKSGDKEY
                     YVQVIDSKSNVLGDKQTVNFGDKSLTYSFISKVKYENKTVNVTEDLPGKDFAKGTYFV
                     NVFDKSELVSKTSFTLK"
     gene            complement(6222..7460)
                     /gene="hutI"
                     /locus_tag="PLQ70_05735"
     CDS             complement(6222..7460)
                     /gene="hutI"
                     /locus_tag="PLQ70_05735"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQE34152.1"
                     /translation="MKTLLINIKELLQVRDASVLKVSGDAMAVLPSIKNAYLLIENDW
                     IADFGSMNDLPKINADKIMDATSKIVLPSWCDSHTHLVYAGHREQEFVDRINGLSYEE
                     IAHRGGGILNSAQLLNETSEDEIYDQSKVRLEEIMRLGTGAVEIKSGYGLTVDGELKM
                     LRIINRLSQNYPITIKATFLGAHAFPLEYANNRQGYIDLIINTMIPKITQNQLADFID
                     VFCEKGYFTVEETEQIMEAGIKFGLKPKIHVNQFNSIGGISAGIKHNALSVDHLEVMT
                     AEDIEALKKTNTMPVALPSCSYFLGIPYTPAREMIAAGLPLALASDFNPGSTPSGNMN
                     FVVATACIQMKMTPEEAINAATINGAYAMNLSKTHGSITIGKKANLIITKSISSFYQL
                     PYSFGSNLIDSIIIEGEVLA"
     gene            complement(7473..8528)
                     /locus_tag="PLQ70_05740"
     CDS             complement(7473..8528)
                     /locus_tag="PLQ70_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HQE34153.1"
                     /translation="MKNYNFLFIILLGITLSNCGSKKNDVNSTFTFDDSKFKAKYLSE
                     ESLELGVLNTNAKEVDSVVYFNNDIKLGSKKGLDKLVFNLKDQKLGYQNLKAIVYFEG
                     ENSEATTRIELVSNVTPKLLKYTIVNTYPHDTASFTEGLEFYKDTLYESTGSGNNCKS
                     YLRKYDYKTGKIYNQTNLDSIYFGEGITFLNNKIFQLTWQNKTGFVYNATTWKLEKTF
                     TFDQDIEGWGMTNDGKYIYQSDGTEKIWKMNPENQKMIDYINVYSSSSKIPKINELEW
                     INGKIYANIWTKDAIAVVNPTNGAVEGILDLSSLRKLIKFPEAEVLNGIAYNPKTKTI
                     FVTGKQWDKMFEITVSE"
     gene            8616..8713
                     /gene="ffs"
                     /locus_tag="PLQ70_05745"
     ncRNA           8616..8713
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLQ70_05745"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8949..>9318)
                     /locus_tag="PLQ70_05750"
     CDS             complement(8949..>9318)
                     /locus_tag="PLQ70_05750"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HQE34154.1"
                     /translation="IDSAQVLQELSEIAKEMQLEDKKSKDLGLTPEEYAFYSVLKDND
                     STSFLDDDKMKDLIHTIVDVIRKNATVDWSKRDDVRAQLRLTVKKILMRYGYPPDVAK
                     MEADRVIAQSESLADVFSRE"
BASE COUNT         3059 a         1566 c         1562 g         3131 t
ORIGIN      
        1 aaaaagggtt gacttttgct aataattcca aaaacgataa aattaaaaat ttcatcaata
       61 ataatttggg gaacgtctat tgttttgaaa aaaaacaata tgaaaaaggg atagaatttt
      121 ataaaaaatc tttagaatac agccaaaaag ctgccgatac aagccaaatt gtatttacga
      181 aactcaatat tgcttgggcc tattttgata ttgatcaata tgaaaatgga ttgccatatc
      241 tagaatacat caacaaatat caagctaagt ttagtgataa atccttaatt ataatactca
      301 atatgctcaa cggaatgtat tccggtcaca aaggcgattt tgctaagggc gaaaactatt
      361 ttttgacagc cataaattta ggaaataaat tgggaaataa gggagatttg tctttttcac
      421 accaagaata ttccaagttt ttatttaaaa aaggagagta caaaaaggcc tatgaaaact
      481 tagtaattta taataaaatc tctgatgaaa tttataacga tgaccttctc aaaaaagcca
      541 atgtagcagg aataaatctc gaattagacg aatacaaaag agaaattgac aaagttgaaa
      601 cagaaataga attacaatcc caaagtctcc ataaatctcg tgtgatagta attttattta
      661 tcattgtatt cctagtatta ttgcttttat tggtttcgct atacaaaaac ttcaatttca
      721 agaaaaagat taatcacgac ctcacattgg caaacgaaca attaattatt gcaaaagaca
      781 aagccataga agcatctcaa gccaaaaccc aatttttatc cacaataagt catgaattgc
      841 gaactcctct ttacggcgtt attggtataa ctaatttgtt gttagacgag cataaagaac
      901 tggccaaaag cccccatttg ggttcgctta aattttcggc acgttattta ttatccttgg
      961 tgaacgacat tttgcaaatt aataaaatcg aagaaaacaa aattatcctt gaaagtttag
     1021 cgtttaacgt ttcggatgaa attcatttag taaaaaatgc actgtctttt attgctcaaa
     1081 gccataagaa tataatatca gtaaaaatag accctgctat tcctgaatat ctaattggcg
     1141 ataagctgcg attggctcaa atattgatga atttagtgag caatgcgcta aaatttacca
     1201 ataatggcga agtaattctt agtgcaaatt tggttaaagt agaagataat ttacatcata
     1261 tagaatttca agtttctgat aatggagtag gaattgatgc agcagaccaa gatcggattt
     1321 ttgagaaatt tgttcaagtg ggacgaaacg acaacgacta tcaaggaaca ggattgggct
     1381 tagccatcgt aaaaagattg ttgggattgt ttaatagtca aattaccctc gaaagtcaag
     1441 taggaattgg caccactttt atatttacaa ttgcctttga acatgactca aacaagacta
     1501 acgaaattat caataatata aaagtcgatt taagttctgg acaattattt aaggttttgg
     1561 tagtagaaga caataaaatc aatcaaacca ttaccaagag aattatcgaa aaaaacaatt
     1621 gcagttgttt tattgtcgat gatggattgc aagctttgga tgtattagaa aaagatgttt
     1681 ttgacattat cttgatggac ataaatatgc cactaattag cggttttgaa accacccgaa
     1741 aaattcgaag caagggcatt catacaccca taattgcttt gacggctttt gccaaagacg
     1801 aaattaccga agaagctata gaagccggaa tgaatgatat tttgataaaa cctttcgagt
     1861 ccttaaagct atttcaggtg atgaatgagc aaataaaaaa aaccaaaacc gctgtttatt
     1921 gacagcggtt ttgggttaat gatataattg tattggttcc tttctattgg ataggaatag
     1981 attttagggt tcttttttta gcaccttctt tttttcctac cacaacatga agaattccgt
     2041 ctttataatt ggcttctatt ttgttttcat caatagtttc gggtaaacta aacgttctgc
     2101 tgaaagattg gtaattaaac tctcttcgga tgaagttttc ttttttgctt acttcttctt
     2161 ttttttcttc tttttctgag gaaatcatca acatatcatt atctacttca actttgaaat
     2221 cttctctttt cattcccgga gctgctagtt caacttcaat ttcagtatcc gtttctttta
     2281 agtttacaga aggcaggtta ctaccaattg ctgcaaaatt tcggtctgtc caatcaaata
     2341 aatcttttgt aataaaatca tcaaaaaaat tatggattga tttactttct aatgacggaa
     2401 acataccgtc ttttttaact aaagttttca tgatattaat tatttaaaaa gttttaaaaa
     2461 tcactatata aagttagtaa ttattcgaaa taattatctt tacgataaca tataattaac
     2521 atttaaaata ctgaaaataa gaaagttatt attaatttat aaataaaaaa aaccgctttt
     2581 tagaatcaac taaaagcggt gtttttgaaa ctgaaattta gaattctaac gaattaatac
     2641 cagcgttttt tgttttgttt agaagcatta gactttctag atttatgcgc tccaccgctt
     2701 cggttagagt ttttttgagc cgttgcggga gctgtttctg gacttcctga atgccaagga
     2761 tagggatgat cagatatggt ttttacctct acttttatca atttttgaat gtctttccag
     2821 tagggctgtt cgtctttact acaaaaagaa atcgcgattc cttcatttcc tgcacgacct
     2881 gttcgaccaa ttctatgaac ataggtttcg ggaatattag gcaaatcaaa attgattaca
     2941 taaggcaatt gatcaatatc aattccacgt gctgcaatat cagtggctac aagaacacca
     3001 acttctttat tcttgaaggc gtctaaaact ctttgacgtg cattttgcga tttgtcaccg
     3061 tgaatggctt cggcaggaat atcttttttt ctcaaggctt taaccacatt atcagcaccg
     3121 tgtttggttc tcgaaaaaac caatacatcc gataagtttt gatttttgat caaatgatac
     3181 aacaagtttc gtttttctcc tttttcgaca aaataaatgt gttgttctac attttcggct
     3241 gtcgatgaaa ctggagaaac ttctactttt tcgggatctt tcaaaaacat ttcggccaat
     3301 tctcgaatgg caatgggcat tgttgctgaa aacaatagcg tttgtctgtt tttgggagtt
     3361 agtttcacga tttttttaac atcgtttatg aatcccatat ctagcatttg atcggcttcg
     3421 tctaggacca aagtgtgtaa actgtcaaaa tcaagaaaac cttgtttgtg caaatcgagc
     3481 aatcttcctg gagttgcaat cagaatatcc actcctttgc gaagcgtatc tacttgagga
     3541 acttgtgaaa caccaccaaa aatagtgagt tgggtcaagt tggtatattt gccataagtg
     3601 tcaaaacttt gtccaatttg aacggctaat tcacgtgttg gagtaacaat taaggctcga
     3661 atttctttgg cttttttaga tgaacctaca attcggtgca attgatggat aattggaatc
     3721 gcaaaagcgg ctgtttttcc agtgccagtt tgtgcgcaac ctatcaagtc ttttcccgcc
     3781 aaaactaatg gaatagcttg ttcttgaata ggagtggggt ttgtgtaacc ttcttcaaat
     3841 acggcctttt gaatactttt tgaaagtgat aaatcttcga ataacatatt tttggtatta
     3901 tacatctagt aattggtaca tagatagtcg tgcaaagata gggttattat ttttggtggt
     3961 cattagttcc taattttagg ccgagcattt gcttttaaca ttttaacagc aaattatttt
     4021 ttttcaatct caaaccccaa aaattgctaa acaatctaaa tttggctatt tgaatttgtg
     4081 aaaatttgtg gtaaaagtaa aaaaagtgaa tgtcctgatt ttaagctcaa tttatcacac
     4141 tattacagac ttcttacggc tcaatactgt tgttcgattt tataaaatca gtaaccaaat
     4201 aaccaataag ttttccaatt aaatgattgt ttttttcata gcataattca ggagcacctt
     4261 cgcaaatatg aagataagcg gcatttttat gtttgccaaa ataggtgata aattgcctca
     4321 attcttcaat agagaaccca cttagagtca ttgcactact ggctacattc ggaatagcat
     4381 caaggtcaac ctcaattccg taaaaatcag tttgaacaaa ttgtgaagct agaattagtt
     4441 cttgattgaa atttttttct ctgcgaatat tgatgctgtc ataagtggta taacgcactc
     4501 ggtcttcaat ttttttgatg atatccaaga cacttttgga ggtataattt tcgtgtaaac
     4561 caaaaatgaa atactttttc aagaaacctt cttcataggc ataggaaaat ccatttccgc
     4621 tatggcgtcc ttccagaatc ctaaaatcgg aatgagcatc aaaattgacg gcattaatgg
     4681 gttttccttt ggctagcgca gttcctttta tattgccata agaattgttg tgaccgccac
     4741 caataacgat aggtattttg cctgctttta tgatattaaa aatgatatga gaaacatctt
     4801 tatctatttt ttctactagt tgactcagtt tggaacgatc atcaatatca ttaaaatcta
     4861 aatctttgac ttccttcatt tcttcgcaaa cattcaattg tcctaaaaca aggatttggc
     4921 tgcctttgca aaaacgattg tgttgaatgt tggctatact actaatagca cattgccagg
     4981 ccgatgctgc tccgggtcgg ccataattag ctctaactcc aatatcttct ggaattccta
     5041 aaagtacata tttagcttcg caagttttta taaattgggc tgggtcaatt ccttttggaa
     5101 ctgtcaacat tttttctcca aattttatct caccacttcg gtgattagta acttttgcaa
     5161 ggtcatttat tgtaaaaagt atcagttttt ccataaaaaa aaataatcct ccaaatataa
     5221 tataaatgtt tagaatccgt tgttaagtca tattatatta ttaaatttgt gcataaaaaa
     5281 aaatagaaaa aatggaagat caacaaaata attcaaattc aagccttaag ataatcattg
     5341 gggtattagc acttctttta gtaggaagtc tgtattatat ttacaagatt tcaacagaag
     5401 cagagcaggt gcaaaccgtt ttaacaacga cagtatctga aaaagaatct gttatgaagg
     5461 atttacaaga attaaaagca acgtatgatg ctgcaatcgc tgaaaacact tcgatgtctg
     5521 aagaattagt caaagaaaga gacaaagtag taaaattgat gaatgatatc gataaatcca
     5581 aaggtgatgt atcaaaatac aaatccgaag tgtcgaaatt gcaaaacaat atgaaggctt
     5641 tgatggctga aaatgatgtc ttgaaaaaac aaaacaaaac cttaaccata caacgcgata
     5701 gtacagtagt ggttttaggc gaagctaaga aatacaatga ggtcctggta ggtcaaaacg
     5761 aagatttgtc taaaacagtc gaaaaagggt caaaattgac agtgttgaat atgcaaactg
     5821 ctgcctacaa aatcagaagc tccggaaaac aaatagctac tgacaaagca ggaagagccg
     5881 atgttcttaa aattagcttt accattgccg aaaatcaaat cgctaaatcg ggtgataaag
     5941 agtattatgt tcaggtgatt gacagtaaaa gtaatgttct tggagacaaa caaaccgtga
     6001 attttggtga taaatcatta acatatagct ttatttctaa agtaaaatat gaaaataaaa
     6061 cagtcaatgt taccgaagat ttaccaggta aagatttcgc aaaaggaact tattttgtaa
     6121 atgtttttga caaaagcgaa ttggtttcta aaaccagttt cactttgaaa taagaatttc
     6181 aagatttata aaagaaagag gagcagatgt tcctcttttt tttatgctaa aacttcacct
     6241 tcaatgatta tagaatcaat caaattgctt ccaaaagagt agggaagttg gtaaaaggaa
     6301 ctaattgatt ttgtaataat gagattggct tttttgccaa tagtaatgct tccgtgggtt
     6361 ttagaaagat tcatagcata agcgccattt attgtagcgg cattgatggc ttcttcggga
     6421 gtcattttca tttggatgca agccgttgct acaacaaaat tcatattgcc agaaggtgtt
     6481 gaacctggat tgaaatcaga agcaagagct aagggcaaac ctgcagcaat catttcacga
     6541 gcaggagtat agggaattcc caaaaaatag gagcaagaag gcaaagctac aggcattgtg
     6601 ttagtctttt tcaaagcttc aatatcttcg gcagtcatta cttcaagatg gtcaacagat
     6661 agagcgttgt gtttaatacc tgctgaaatg ccaccaatag agttgaattg attcacgtga
     6721 atttttggtt ttaaaccaaa tttaattcca gcttccataa tttgctcagt ttcttcaaca
     6781 gtaaaatagc ccttttcgca aaaaacatct ataaaatcag ccaattggtt ttgggtaatt
     6841 ttaggaatca ttgtattgat aattaaatca atataacctt gtcggttatt ggcatattcc
     6901 aatggaaaag cgtgagcgcc aagaaaagta gcttttatag ttatcggata gttttgcgac
     6961 aagcgattga tgatgcgtag cattttcaat tcgccatcaa ctgtcagtcc gtagcccgat
     7021 tttatttcta cagcacccgt tcccaatcgc attatttctt cgagtcgaac tttcgattgg
     7081 tcgtaaattt catcctccga agtttcgttg agtaattgag ccgaatttaa aatgcctcca
     7141 ccacgatggg caatttcttc ataagaaagc ccattaattc tatctacaaa ttcttgttct
     7201 cgatggccag cataaaccag atgagtgtgg ctgtcgcacc aagaaggcaa aactattttg
     7261 cttgtagcat ccataatttt gtcggcattg attttgggta aatcattcat tgaaccaaaa
     7321 tcagcaatcc aatcattttc gattaacaaa taagcatttt ttatagaggg aagaaccgcc
     7381 attgcatcac cagaaacctt caaaacgctt gcgtctcgaa cttgaagtaa ttccttgatg
     7441 ttgatgagta gcgttttcat taggcagtga aattattccg aaaccgtaat ttcgaacatt
     7501 ttgtcccact gttttccagt tacaaaaatg gttttggttt tggggttgta ggcaattccg
     7561 ttcagtactt cggcttctgg aaatttaatt agctttctta aactcgaaag atctaaaatt
     7621 ccttctactg caccgtttgt tggatttaca actgcaatag catcttttgt ccatatattg
     7681 gcataaatct ttccattaat ccattctagt tcattgattt ttggaatttt agaactcgaa
     7741 gaataaacat tgatataatc aatcattttt tgattttcgg gattcatttt ccatattttt
     7801 tcggttccat ctgattggta aatgtatttt ccatcattgg tcattcccca accttcaata
     7861 tcttggtcaa aagtgaatgt tttttcaagt ttccaagtag tggcattata aacaaaaccc
     7921 gttttgtttt gccaagtcaa ttggaaaatt ttgttgttca aaaaagtaat tccttcgcca
     7981 aaataaatag aatcaagatt ggtttggttg taaattttcc ccgttttata atcgtatttt
     8041 cttaaataag atttgcaatt atttcctgaa ccagtacttt catatagagt atctttataa
     8101 aactccaatc cttcggtaaa tgaagcggta tcgtgtggat aagtgtttac gatggtatat
     8161 tttaataatt taggggttac attcgaaacc aactctattc ttgttgtcgc ttcagagttt
     8221 tcgccttcaa aataaactat agctttcagg ttttgatagc ctaatttttg gtcttttaga
     8281 ttaaaaacta gtttgtcaag cccttttttt gaacctaatt ttatatcgtt attgaagtac
     8341 accacgctat ctacttcctt tgcatttgta tttaaaactc ccaattccaa agactcttct
     8401 gaaaggtatt tggctttaaa tttcgaatca tcgaaagtaa acgtagaatt tacatcattt
     8461 tttttactac cgcaattcga taaagtgatt cctaataaaa tgataaatag gaagttataa
     8521 tttttcataa aacaaatttt taatgatgca atatacaacg atattttaaa ggcacaaagt
     8581 acttgcaaaa atataaaaag agtgtatatt tgcaccggca agtcctacac gaccagctcc
     8641 tgcagactcc cccaggatgg gaacatagca agggtacgcg gttgagcggt gcgatgtagg
     8701 tcgcttgcca ttttttatta ctatccagtt caaaagaatc cttttgtatg tactaatctt
     8761 ccctcggaag atttttttgt ttttaggagc tatagatttt ttgatagacc ttccttcatt
     8821 atcgctgctt tcctttttta gaccagttat tcttttgatt tctaaataaa aaaaatcggc
     8881 ttagaaagct ctaaaccgat tggtatatgt gttaactatt gtatcttaaa gcggttttat
     8941 tagtttaatt actctctact aaatacatca gccaaactct cactttgagc aatcactcta
     9001 tcagcttcca ttttagcaac gtcaggagga taaccataac gcattaatat tttctttacg
     9061 gtcaaacgca attgtgccct tacatcatca cgcttgctcc aatctactgt tgcatttttt
     9121 cggattacat ctacaatagt gtgaatcaaa tctttcatct tatcatcatc taaaaaggag
     9181 gtagaatcat tgtctttcaa aacactataa aaagcatact cttcaggtgt tagccctaaa
     9241 tctttagatt ttttatcttc taactgcatt tcttttgcaa tctctgataa ctcttgtaat
     9301 acctgtgcag aatcaatt
//
LOCUS       DAOSNN010000083         9239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7428_length_9239_cov_4.876198, whole genome shotgun sequence.
ACCESSION   DAOSNN010000083 DAOSNN010000000
VERSION     DAOSNN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7428_length_9239_cov_4.876198"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLQ70_05755"
     CDS             <1..571
                     /locus_tag="PLQ70_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34155.1"
                     /translation="ENQIISSTLSNNWQILNNYISGGNYYIFNSLRADSFVSNNIIVC
                     TSGVSIFSNCYNVASNNLFLAQNATYTALNISGSASNVVFSNNLTYNTSGTTLATLPG
                     TGNYNNTNPSFVLAPTLTSYSVTNDYHSSNATLLGTDGTTIGIYGLNFPFNKRGYSFS
                     MPYIESMTILNPTIPANGTLQINLTAKSN"
     gene            621..1376
                     /locus_tag="PLQ70_05760"
     CDS             621..1376
                     /locus_tag="PLQ70_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE34156.1"
                     /translation="MKSILTLLVLLLFRNLYSQSDINQAEYYFDTDPGFGSANAITVS
                     AGNTFTLTNYSIATQNLPEGIHFLYIRCKDANNKWSLPIRKVMYIHPNLNATSPITTA
                     EYFVDTDPGVGLANAISLTAGTTVNQSFDVVTNGLAVGNHNLFVRVKNQDGVWSVISK
                     KVFTLTSPLSVSENMFFEAKVYPNPTTGFVTIQLAQNESIRKIEIIDMLGKIVVSQNS
                     SSNTIDLETLHSGIYFIKAFADEKVFLKKIIKQ"
     gene            1384..3345
                     /locus_tag="PLQ70_05765"
     CDS             1384..3345
                     /locus_tag="PLQ70_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HQE34157.1"
                     /translation="MKKLLLIILTAISASAFGQNFTYNGINYTINDATNFKVAVGSNT
                     GFSGDANIPSTVVYNSQNYEVNAIGVNAFYNCMGLTSATIPNSVTTIGEAAFRECRGL
                     TSVTIPNSVITIGESAFKECRILTSIAIPNSVSTIGADAFYNCRILTSITIPNSVTSI
                     GEGAFEFCVSLPSVTIPSSITAIQGYVFANCFSLTSVTIPASVTTIGEAAFYYCYNLP
                     SIVIPNSVTSIGTFAFRKCLSLASVTIPDSITAIANETFGECASLTSVTIPNSVTSIG
                     NVAFNSCSSLTSLTIPNSVTSIGEGAFQNCSKLVSVTIPNSVTTMGSYAFYNCIKLAS
                     VTIPDSMTSIGTGIFYECSGLTSVTIPNSVTSIGSTAFYRCYKLTSITIPNSVTSIGA
                     SAFSGCYGLTSFTIPDSVTSIGSAAFHQCIALPSIIIPNSVTSLATDTFAYCAGLTSI
                     TIPNSVTSIGDTAFQNCIGLTSITFPDSVTSIGAYALYNCKKLASVTIPKSVTVIKNG
                     AFSYCPVLTTVNCYITTPLVINSTVFQEVNQAICALNVPSGAETMYRTAAIWTNFNPI
                     NGTLATDSFDIKNNLSVYPNPTTGIISVSNQDMLAIDKVEIIDVFGKTVMITTTINSQ
                     IDLGGLPQGVYIFKIHSKETIFVKKIIKQ"
     gene            3422..5323
                     /locus_tag="PLQ70_05770"
     CDS             3422..5323
                     /locus_tag="PLQ70_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQE34158.1"
                     /translation="MKTTFLVIILYLTTTVVAAQIDTLKIQKMMDDAYALEVSHPQKA
                     LQLYKKTHQLSVQSNFKNGAYKSLLYRGFIYNDIGKYDSAKYYFDKTIDYCKKSKNLI
                     GEAKGYANLANTYQFKGEYPAAINNYIKSIKIFEKTKDSAIISQSYQNLSAVYTQFIN
                     RKLEFFYLKKAEQFMPKANKVQLALLYGDIGLGFIRYNDFAESFSYFKKAEAIAVKEN
                     NNELWFYVYRNFGEYYRIKKEYKKAIPYYEKAFKLADFAVDIIRKNDLIYIVSGLYLK
                     NDNYSKALELANQSLELSRKIGASEIEYKTLKRLSEIHNKFNQPQKAYDFLEKSYVLK
                     DTFFSQNHLKETTLLQTKFETEKKDKSIAEQQIKLKQQELDLIKSQKEKQLYFIASLC
                     LIIVSLGIWYFFRQRQKLKNQEIETLKQNQEIAKLEALIDGEEKERRRIAQELHDGLN
                     GDLSAIKYRLSTLEESGLSAIDTENLNKVIDMIDESCAQVRSISHNLMPSSILEYGLI
                     ESIREYCIKIKSSDNFKIDFQIFGSYIALSKKNETVIYRIIQELVTNILKHSKATEAM
                     IQFNYREDELFITVEDNGIGFDKNAVSKGIGHQNIQTRIDFLNANLDVNTSADGTSYT
                     ISIDLNKVK"
     gene            5320..5949
                     /locus_tag="PLQ70_05775"
     CDS             5320..5949
                     /locus_tag="PLQ70_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQE34159.1"
                     /translation="MIQIAITDDHTIVIEGIKTMLKSNKEIEVVQSFENLKDTFEQLD
                     PSVEILLLDINLPDGNGIQACKELLEKYSHLKIIALTNFEDSIFIKQILKNGALGYLL
                     KNTSKTELTEALKQVQLGNRYLPKNISDILLNDSIGLENSNYFIPKLTTREKEILALI
                     IQEFTTEEMAKKLYVSTKTVESHRSNLIQKLGVKNTAGLVRVAFEKGLI"
     gene            complement(6020..6781)
                     /locus_tag="PLQ70_05780"
     CDS             complement(6020..6781)
                     /locus_tag="PLQ70_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HQE34160.1"
                     /translation="MSAAKNINISLQIWRQKNSKEKGKMETYKLNDVSTASSFLEMLD
                     QLNEQLVNERKEPVAFDHDCREGICGMCSLYINGRAHGPDTGITTCQLHMRMFNDGDT
                     IVIEPWRSAAFPVIKDLIVDRTSFDRIQQAGGFVSVNTSGNTIDANATPINKQDADKA
                     FEAAACIGCGACVATCKNGSAMLFVGAKVSQYALLPQGRIEATDRVLNMVRQMDEEGF
                     GNCTNTGACEIECPKGISLENIARMNREYLSASLK"
     gene            complement(6828..7202)
                     /locus_tag="PLQ70_05785"
     CDS             complement(6828..7202)
                     /locus_tag="PLQ70_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQE34161.1"
                     /translation="MGEIKSYKDLLIWQKGIDIVCLTYKLVASFPKDELYALSSQIKR
                     SSVSIPSNIAEGYGRQSTQSYIQFIKIARGSLCELETQLLVANKLDFINDEILFLELS
                     NQIIEESKMINSFLNKLESSKQ"
     gene            complement(7230..9239)
                     /locus_tag="PLQ70_05790"
     CDS             complement(7230..9239)
                     /locus_tag="PLQ70_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HQE34162.1"
                     /translation="MKLDSKIPEGHISQKWTDYKDHLRLVAPNNRPKIDIIVVGTGLA
                     GASAAASFAEMGYNVKAFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSTFRLFYDTIK
                     GGDYRAREANVHRLAEVSGNIIDQCVAQGVPFARDYGGMLDNRSFGGTQVQRTFYAAG
                     QTGQQLLLGAYSALSRQIGLGKIDMYNRHEMLELVKVDGKARGIIARNLITGELERHS
                     AHAVIIATGGYGNVYFLSTNAMGSNVTAGWKIHKQGALFANPCYVQIHPTCIPVHGTN
                     QSKLTLMSESLRNSGRIWVPKKKEDAEAIRAGKLKPTQIAEEDRDYYLERKYPAFGNL
                     VPRDVASRAGKEVCDEGRGIEANDTNEGVYLDFATEVQSKGRQTAYAKGNHNPSQEEI
                     LTLGKKWLEEKYGNLFTMYQKITDENPYETPMKIYPAVHYTMGGVWVDYNLQSTIPGC
                     FVAGEANFSDHGANRLGASALMQGLADGYFVLPYTVSNYLADDIRTGKISTDLPEFVA
                     AEKNVKDSIDKFLSNNGTKTVDHFHKRLGHIMWNKVGMGRNEKGLTEAISEIAALKEE
                     FYKEVYVPGSSDELNPELEKALRVADFIELGQLMAMDALQRKESCGGHFREEYQDSEG
                     ETLRDDENFSFVGAWENKGYDVTKSELHKEELKYEFIKIAARNYK"
BASE COUNT         3064 a         1710 c         1437 g         3028 t
ORIGIN      
        1 tgaaaatcag ataatctcat caaccttatc taacaactgg caaatactca ataattatat
       61 ttctggaggc aattactata tctttaatag tttaagagct gactcatttg tttctaataa
      121 cattattgta tgtactagtg gtgtaagcat tttttctaat tgctataatg tagccagcaa
      181 taatttattt cttgcccaaa atgctactta tacagcactt aatatttcag gaagtgcgtc
      241 gaatgttgta tttagcaaca atcttactta caacacttca ggcactactt tggcaacact
      301 accgggaaca gggaattata ataacacaaa tccttctttt gtattggcgc caacactaac
      361 tagctattct gtcaccaatg attatcactc tagcaatgcg acactattgg gcactgacgg
      421 aacaacaatt ggaatttatg gattaaattt tcctttcaac aaaagaggtt attcattttc
      481 aatgccttac atagaatcaa tgacaatact aaacccaaca atacctgcaa atggaacatt
      541 gcaaattaat ttaactgcca aatcgaattg atttttgctg gagttgtttt tttaaaaatt
      601 aatctaaaaa atccattgcg atgaaatcaa tcctaacctt attagtacta ttgctcttta
      661 gaaatctcta ttctcaaagc gacatcaacc aagccgaata ttattttgat accgatccag
      721 gttttggcag tgccaatgct attacggtga gtgctggaaa tacttttaca cttactaatt
      781 atagtattgc cacacaaaac ctccccgaag gcattcattt tctatacatc agatgcaaag
      841 atgccaacaa caaatggagt ttaccaattc gaaaagtaat gtacattcat cctaatctta
      901 atgccacttc gcctattaca acagccgaat attttgtaga taccgatcca ggagttggcc
      961 tagcaaatgc aataagccta acagctggaa ctacagtaaa tcaaagtttt gacgtagtta
     1021 ctaatggttt agcggtaggc aatcacaatc tatttgtaag agtaaaaaat caggatggtg
     1081 tttggagtgt cattagcaaa aaggttttta cgttgaccag cccactttct gtttccgaaa
     1141 atatgttttt tgaagcaaaa gtgtatccca atcccacgac tggatttgta actattcaac
     1201 tggcgcaaaa tgaaagcatt cgcaaaatcg aaattataga tatgcttggc aaaatagtgg
     1261 tttcgcaaaa tagttcgtca aacacaatag accttgaaac cttacactcg ggaatttatt
     1321 tcatcaaagc ttttgccgat gaaaaagtgt ttttgaagaa aataattaaa cagtaaaaaa
     1381 attatgaaaa aattattact tataatactt acagcaataa gtgccagtgc ttttggacaa
     1441 aattttacgt ataacggtat taattatacc attaacgatg ctaccaattt taaagttgct
     1501 gttggaagca atactggttt ttcgggtgat gctaacatcc caagtactgt tgtatataat
     1561 agccaaaatt atgaggtaaa tgctatcgga gttaatgctt tttataattg tatggggtta
     1621 acttctgcaa ctattcctaa ttcggttact actattggag aagctgcttt tagagaatgt
     1681 agaggtttaa catcggttac tattcctaat tcggttatta ctattggaga atctgctttt
     1741 aaagaatgta gaattttaac ttcgattgct attcctaatt cggtctcaac tattggagca
     1801 gatgcttttt acaattgccg aattttaact tcaattacta ttcctaattc ggttacttct
     1861 attggcgagg gggcttttga attttgtgta agtttgccat cggtaacaat ccctagttct
     1921 attacagcta ttcaagggta tgtttttgcc aattgttttt ccttaacttc ggttacaatt
     1981 cctgcctcgg ttacaactat tggcgaggcg gctttttatt attgttataa tttaccatcg
     2041 attgttattc ctaattctgt tacttctatc ggaacatttg cctttagaaa atgtttaagt
     2101 ttagcatcgg taactatccc tgattcgatt actgctatag caaatgaaac ttttggagaa
     2161 tgtgcaagtt taacgtctgt aaccatacca aattcagtaa cttctattgg caatgtcgct
     2221 tttaattcct gttcaagttt aacttcactt accattccta attcggtaac gtctattggc
     2281 gagggtgcat ttcaaaactg ttcaaaactg gtttcggtta ctattcccaa ttcggttaca
     2341 actatggggt cttatgcctt ttataattgt ataaaattag catcggttac cattcctgat
     2401 tcaatgactt ctattggaac tggaattttt tatgaatgtt caggattaac ttcggtcacg
     2461 atcccaaatt cagttacatc tataggtagt actgcctttt atcgttgtta taaattaact
     2521 tccatcacta ttcctaattc ggttacatct attggagcca gcgcattctc tggctgctat
     2581 ggcttaactt cttttaccat tccagattca gttacttcta taggtagtgc tgcttttcat
     2641 caatgtattg ctttaccttc tataattatc cccaattcgg ttactagtct tgcaacggat
     2701 acttttgcct attgtgcggg attaacctca ataacaattc caaattcggt tacttcaatc
     2761 ggagatacgg cttttcaaaa ttgtataggt ttaacatcta taacctttcc tgattctgta
     2821 acttcaatag gtgcttatgc cttatataat tgcaaaaaat tagcttcagt aacaattcca
     2881 aaatcagtta cggttatcaa aaatggtgct ttttcatact gcccggtcct aactacggta
     2941 aactgctata tcacaacacc tttagtaatt aattctactg tatttcaaga agttaatcaa
     3001 gcaatatgtg ccttaaatgt gccttcggga gccgaaacaa tgtatagaac tgccgcaatt
     3061 tggaccaatt ttaacccaat aaacggcact ttggctactg atagttttga tattaaaaac
     3121 aatctttctg tttatccaaa tccaacaaca ggaattattt ctgtttcaaa tcaggatatg
     3181 ctagccattg acaaggttga aattatagat gtttttggaa aaacggtaat gattacaaca
     3241 acaataaatt ctcaaataga tcttggtggg ttacctcagg gagtttatat ttttaaaata
     3301 cattctaaag aaaccatttt tgttaaaaag attatcaaac agtaacagct tacaggatat
     3361 tttatagaaa aaagcggcat actattacta tatttacaac tttagaccaa atatttgtgc
     3421 aatgaaaaca acctttttag tcattatttt atatttaacg acaactgttg tagcagcgca
     3481 aattgatacg ctcaaaatcc aaaaaatgat ggatgatgct tatgccttgg aagtaagtca
     3541 tccacaaaaa gcattgcaat tgtataaaaa aacacaccaa ttaagtgtac aatcaaactt
     3601 caagaatggt gcctataaat cactattata tagaggattt atttacaatg atattggaaa
     3661 atatgattct gctaaatatt attttgacaa aacaattgat tattgtaaaa aatcaaaaaa
     3721 tctaatagga gaagctaaag gttatgcaaa tttagctaac acctatcaat tcaaaggcga
     3781 atatccagca gcaataaata attatattaa atctatcaaa atatttgaaa aaactaaaga
     3841 tagtgccata attagtcagt cttatcaaaa tctatcagct gtttatacac aattcataaa
     3901 cagaaagtta gagttttttt atcttaaaaa agcagaacaa tttatgccta aagccaataa
     3961 agtgcaatta gcattattgt atggcgacat tggtcttgga tttataagat ataatgattt
     4021 tgctgaaagt ttttcttatt tcaaaaaagc agaagcaatt gctgtcaaag aaaacaataa
     4081 cgaattatgg ttttatgtct atagaaattt tggagaatat tacagaataa aaaaagagta
     4141 taaaaaagct attccatact acgaaaaagc atttaaactt gctgattttg ctgttgatat
     4201 tattagaaaa aatgatttga tttacattgt tagtggttta tacctaaaaa atgacaatta
     4261 ttccaaagct ttagaattag ccaatcaatc tttagaactt tccagaaaaa ttggggcaag
     4321 tgaaattgaa tacaaaacat taaaacgtct ttcggaaatt cataataagt ttaaccaacc
     4381 tcaaaaagcg tatgattttt tagaaaaaag ttatgttctt aaagatactt ttttttctca
     4441 aaatcattta aaagagacaa ctttacttca aaccaaattt gaaaccgaaa aaaaagacaa
     4501 atctattgct gaacaacaaa taaaactcaa acaacaagaa ttagatttaa tcaaaagcca
     4561 aaaagaaaaa caactctatt ttattgccag cctttgttta attatagtgt ctttgggaat
     4621 ttggtatttc ttcagacaaa gacaaaagct taaaaaccaa gaaattgaaa ctttaaaaca
     4681 aaaccaagaa attgccaaat tagaagcatt aatagacggc gaagaaaaag aaagacgtag
     4741 aattgctcaa gaattgcacg atggcttaaa cggcgattta tcggccataa aataccgttt
     4801 gtcaacactg gaagaatcgg gtttaagtgc tattgatact gaaaacttga ataaagttat
     4861 cgatatgata gacgaatctt gtgcacaagt acgcagcatt tcgcataatt tgatgccttc
     4921 gtcgatacta gaatatggtt taatagaaag tattcgcgaa tattgtatta aaattaaaag
     4981 ttccgataat ttcaaaattg attttcaaat ctttggcagt tacatcgctt tatccaagaa
     5041 aaatgaaacg gtaatttata gaattattca agaattggtg accaatattc taaaacattc
     5101 caaagccacc gaggcaatga ttcaattcaa ttatagagaa gatgaacttt ttataaccgt
     5161 agaagataac ggaattggtt ttgacaaaaa tgcggtttca aaaggaattg gacatcaaaa
     5221 cattcaaacc cgaattgatt ttttgaatgc caatttagat gtgaatacat cagccgatgg
     5281 aacatcttac accatttcaa ttgatttaaa caaagtaaaa tgattcaaat agccataaca
     5341 gacgaccaca caattgtaat cgaaggcata aaaacaatgc taaaatcgaa caaagaaatt
     5401 gaagtcgttc aatcttttga aaacctaaaa gacacgtttg aacaattaga tccttcagta
     5461 gaaattttgc tattagacat caatttaccc gacggcaatg gcattcaggc atgcaaagaa
     5521 ttattagaaa aatattctca cttaaaaatc atagccttaa ctaattttga agacagtatt
     5581 tttataaaac aaattctaaa aaacggagct ttgggatatt tgctcaaaaa caccagtaaa
     5641 actgaattga ccgaagcctt aaaacaagtt caattaggca atcgctattt gccaaaaaac
     5701 ataagtgaca ttctgctaaa cgactcgatt ggacttgaaa attctaacta tttcatccca
     5761 aaattaacga ctcgagaaaa agaaattcta gctttaataa tccaggaatt taccaccgag
     5821 gaaatggcaa aaaaacttta tgtaagcacc aaaaccgtag aatcacaccg cagtaatcta
     5881 atccagaaac tgggcgtgaa aaataccgct ggattggttc gtgtggcttt tgaaaaagga
     5941 ttgatttaaa caaaaaaagc gtcccaattt ctcgggacgc tttttacttt ttgccttttg
     6001 gctactgcct aaaaagctat tatttcaaac ttgccgataa atattctcta ttcatacgag
     6061 cgatgttttc caaagaaatt cctttaggac attcgatttc gcaagctcca gtattggtac
     6121 agttaccaaa accttcttca tccatttgac gaaccatatt caaaacacgg tctgtagctt
     6181 caattcttcc ttgtggtaac aaagcatatt gagatacttt tgctccaaca aacaacatag
     6241 cagaaccatt tttacaagta gctacacaag caccacaacc gatacaagcc gctgcttcaa
     6301 aagctttgtc tgcatcttgt ttgttgattg gagttgcatt tgcatcgata gtgtttccag
     6361 aagtattcac cgaaacgaaa cctcctgctt gttgaattct gtcgaaagaa gttctgtcca
     6421 caatcaaatc tttaataaca gggaaagctg ctgaacgcca aggctcgata acgatagtat
     6481 caccatcatt aaacattctc atatgcaatt gacaagtagt gattccagta tctggtccgt
     6541 gtgcacgtcc gttgatatac aaagaacaca ttccgcaaat tccttcacga cagtcgtggt
     6601 caaatgctac tggttctttt ctttcgttta ctaattgctc atttaattgg tctaacattt
     6661 ccaaaaatga acttgccgta gaaacatcgt ttaatttgta tgtttccatt tttccttttt
     6721 ctttggagtt tttctgacgc caaatttgaa ggcttatatt gatatttttt gctgcactca
     6781 taatttttat ttttagccaa aagccataag gcaaaaggca ttagtaacta ttgtttactt
     6841 gattctaatt tatttaaaaa ggaattaatc attttgcttt cttcaataat ctgatttgat
     6901 aactctaaaa acaatatttc atcattaata aaatccaatt tatttgccac taataattgt
     6961 gtttctaatt cacacaaaga acctcgagct attttaataa attgaatata actttgagtt
     7021 gattgtcttc catatccttc tgcaatattt gaaggaattg acactgaact tctttttatt
     7081 tggcttgaca aagcgtataa ttcatctttt ggaaaactag caactaattt atatgtcaaa
     7141 cagacaatat caatcccttt ttgccaaatc aataaatctt tatacgattt tatctctccc
     7201 ataactattg ccttttggct tttgcctaat tacttatagt ttcttgcggc tattttaata
     7261 aactcgtatt tcaattcctc tttgtgaagt tccgattttg taacatcgta tcctttgttt
     7321 tcccaagctc ctacgaatga gaagttttca tcatcacgaa gtgtttctcc ttctgaatcc
     7381 tgatattctt cacggaagtg accaccacaa gattctttac gctgcaaagc atccatcgcc
     7441 atcaattgtc ccaattctat gaaatcggca acacgaagtg ctttttccaa ttcaggattt
     7501 aattcatctg aacttccagg aacataaact tctttgtaga attcttcttt taaagcggca
     7561 atttctgaaa tggcttctgt caaacctttt tcattacgac ccattcctac tttattccac
     7621 ataatatgac ctaaccgttt gtggaaatga tccacggttt tagttccatt attagacaag
     7681 aatttatcga tagaatcttt tacatttttc tcagctgcaa cgaattctgg taaatcagta
     7741 gagatttttc cagtacgaat atcatcagcc aaataattag aaaccgtgta aggcaataca
     7801 aaatagccat cagccaaacc ttgcatcaac gccgaagctc ccaaacggtt cgctccgtga
     7861 tcagagaagt tagcctctcc tgcaacgaaa caacccggaa tagtagattg taagttataa
     7921 tcaacccaaa caccacccat tgtatagtga accgcaggat aaattttcat tggagtttca
     7981 taaggattct catctgtgat tttttggtac atagtaaaca agttaccgta tttttcctcc
     8041 aaccattttt ttcctaaagt aagaatttct tcttgagaag gattatgatt tcctttagcg
     8101 taagcggttt gtcttccttt agattgaacc tctgtagcaa aatccaaata aacaccttcg
     8161 ttagtatcgt tagcttcaat tccgcggcct tcgtcacaaa cttcttttcc agctcttgac
     8221 gcaacgtcac gaggaactaa attaccaaat gctgggtatt ttctttctaa gtagtaatct
     8281 ctatcttctt cggcaatttg tgttggtttc aatttacctg cacgaatcgc ttcagcatct
     8341 tcttttttct ttggaaccca aatacgtcct gagtttctta acgactccga catcaatgtc
     8401 aatttagatt gattggttcc gtgaactgga atacaagttg ggtgaatttg tacgtaacaa
     8461 ggattggcga acaaagctcc ttgtttgtga attttccaac ccgcagttac atttgatccc
     8521 attgcattgg tagaaaggaa ataaacattt ccgtaacctc cagttgcaat aattacagcg
     8581 tgagcagaat gtctttctaa ttctcctgta attaaattac gagcaatgat tccacgagct
     8641 tttccatcca cttttaccaa ttctaacatt tcgtgacggt tgtacatatc gatttttcct
     8701 aaaccaattt gtcttgatag agcagaatat gctcctaata ataattgctg ccctgtttgt
     8761 cccgcagcat aaaaagtacg ctgtacctgc gtaccaccaa aagaacggtt gtctaacatt
     8821 ccaccataat cacgggcaaa aggaactcct tgagccacac attggtcgat gatgtttcca
     8881 gaaacttcag ctaaacggtg aacgtttgct tcacgtgctc tgtaatctcc tcctttaatg
     8941 gtatcgtaaa acaaacggaa agtactatct ccgtcatttt gataattttt tgcagcgttg
     9001 atacctcctt gtgcagcgat ggagtgcgca cgacgcggag aatcctgata acaaaatgct
     9061 tttacattat aacccatttc agcaaaagat gcagctgccg aagcaccagc taatcctgta
     9121 cctacaacga tgatatctat ttttggtcgg ttatttggag caactaatcg taagtgatct
     9181 ttataatcag tccatttttg tgaaatgtga ccttctggta ttttagaatc tagtttcat
//
LOCUS       DAOSNN010000084         9213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7456_length_9213_cov_5.672745, whole genome shotgun sequence.
ACCESSION   DAOSNN010000084 DAOSNN010000000
VERSION     DAOSNN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7456_length_9213_cov_5.672745"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /gene="bshC"
                     /locus_tag="PLQ70_05795"
     CDS             <1..645
                     /gene="bshC"
                     /locus_tag="PLQ70_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HQE34163.1"
                     /translation="PEKFSPNVIMRPLYQEVILPNLCYIGGGGEIAYWLELKSFFNAV
                     NVTFPVLLLRNSVLLATEKQVKKADKLGLSWSDLFSKQTDLVNQKTATLSEFPIDFRI
                     QKEHLRKQFDTLLAIANQTDASFLGAVKAQEAKQIKGLENLEKRLLKAQKRKHSETLE
                     RITDLQNELFPNQSLQERQANFSEFYLENGKDLIHKLITTLKPLDSKFEIVILS"
     gene            718..1989
                     /locus_tag="PLQ70_05800"
     CDS             718..1989
                     /locus_tag="PLQ70_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HQE34164.1"
                     /translation="MTKERLTSLDVFRGLTVIMMTIVNNPGSWVAIYPPLEHAEWNGW
                     TPTDLIFPSFIFIMGTAIPFAMPNKKFNSTVFNKIMVRSLRLFCLGLFLNFFNDLEFF
                     GWVGMPLLIGRLAITFAVAYALLGKFSLKIKTVLAISVLTILLFLAYFGISDYQEVRI
                     PGVLQRIGLVYFFSSILYLKSNIRTQILVVTTLLIGYWALMTLVPVPGIGAANLEKGT
                     NLAAYIDNLFLNNHMWSRSKTWDPEGILITLPAIASGIIGMLVGQLLNHHWPKIEIVK
                     KMILIGFSLLILGLLWDILFPINKLLWSSSFVLFTSGFASLSLSFLYYVIDILHYKKG
                     TKLFLIWGLNPIIVFFFSGIIPRVLTMTKVEHPVFPGKPLGFQSYFYNYGIVPLFENP
                     KDASLTYALIYTVFWSIILWVFYKNKLFFKV"
     gene            2109..2528
                     /locus_tag="PLQ70_05805"
     CDS             2109..2528
                     /locus_tag="PLQ70_05805"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQE34165.1"
                     /translation="MASNRTFTMIKPDAVKNGHIGNILAMITNAGYKIVSLKLTQLTV
                     ADAQAFYAVHAERPFYGELVEFMSSGPIVAAILEKENAVDDFRTLIGATNPAQAAEGT
                     IRKLYATSMGENAVHGSDSDENAAIEGAFHFAGREQF"
     assembly_gap    2615..2714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2715..2980)
                     /locus_tag="PLQ70_05810"
     CDS             complement(<2715..2980)
                     /locus_tag="PLQ70_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HQE34166.1"
                     /translation="MAKRLNNQSTIGAILNEIIQVNKLQPGMDQIDVREAWKNLMGNG
                     VNHYTKNVVLKGSTLYVELSSAVLREELTHGKSKIVAMINEELRR"
     gene            complement(2961..3404)
                     /locus_tag="PLQ70_05815"
     CDS             complement(2961..3404)
                     /locus_tag="PLQ70_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34167.1"
                     /translation="MKNIIGFFFVAFLFISCQQKIKPEDIAKTNGYWEIEKVVFEDGK
                     SKDYTVNDSYDYFQIGKNNSGFRKKVMPQLDGTFLVNDTQESVKVRFEKDKVFFDYVT
                     SYAKWSEELTAISDDKMVFLNAEKKEYHYKKATPINIESDGKKTK"
     gene            3627..4691
                     /locus_tag="PLQ70_05820"
     CDS             3627..4691
                     /locus_tag="PLQ70_05820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQE34168.1"
                     /translation="MKKIIYSLLIAICCLGCSSESTKPVPSPNETLYFPPLSGDVWET
                     KSIADLGWKQAAVQPLLDYLELKNSKGFIILVNGRIVMENYFNGHSATTPWYWASAGK
                     TLTATMTGIAQQEGLLNINNKVSDYIGTGWTSLPLAKENLITNKHLLTMTSGLDDTTD
                     DITPAKLIYKADAGTRWAYHNVYVKLQDVIAKASGQTWPTYFNSKLRDKIGMTGTWIQ
                     DGNNNVYWSTTRSMARFGLLMYNKGKWENSTILNETYFNEATNTSQNINLSYGYLWWL
                     NGKTSYHLPQSQLQLNGSIIPSGPNDMFMALGKNDQKIYVIPSKKMVVIRMGDAADNV
                     NLALSDFDDVLWQKINALYQ"
     gene            complement(4927..5880)
                     /gene="ftsY"
                     /locus_tag="PLQ70_05825"
     CDS             complement(4927..5880)
                     /gene="ftsY"
                     /locus_tag="PLQ70_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQE34169.1"
                     /translation="MSFFKKLFSSEKKESLDKGLEKSKTSFFSKLTKAVAGKSKVDDE
                     VLDNLEEILVSSDVGVNTTLKIITRIEKRVSEDKFLGTDELNQILREEIAGLLSETNS
                     GEATEFVIPIATKPYVLMVVGVNGVGKTTTIGKLAYQFKKAGFKVVLGAADTFRAAAI
                     DQLQIWADRVGVPIVRQNMGSDPASVAFDTLQSAVAQNADVVIIDTAGRLHNKINLMN
                     ELTKVKRVMQKVVGDAPHDVLLVIDGSTGQNAFEQAKQFTAATEVTSLAVTKLDGTAK
                     GGVVIGISDQFQIPVKYIGVGEGIEDLQVFNKYEFVDSFFK"
     gene            complement(6025..6177)
                     /locus_tag="PLQ70_05830"
     CDS             complement(6025..6177)
                     /locus_tag="PLQ70_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013997708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HQE34170.1"
                     /translation="MAKKTVASLQTSSKRLSKAIKMVKSPKTGAYTFVESIMAPEAVD
                     EFLKKK"
     gene            complement(6221..6409)
                     /gene="rpmG"
                     /locus_tag="PLQ70_05835"
     CDS             complement(6221..6409)
                     /gene="rpmG"
                     /locus_tag="PLQ70_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQE34171.1"
                     /translation="MAKKAKGNRIQVILECTEHKTTGVAGTSRYITTKNKKNTPDRLE
                     IKKFNPILKRVTVHKEIK"
     gene            complement(6436..6672)
                     /gene="rpmB"
                     /locus_tag="PLQ70_05840"
     CDS             complement(6436..6672)
                     /gene="rpmB"
                     /locus_tag="PLQ70_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQE34172.1"
                     /translation="MSRVCDLTGKRAMVGNNVSHAMNKTKRKFSVNLVKKRFYLPEED
                     RWITLRVAASTVKTINKNGIAAVLKKAQAGGFIK"
     gene            complement(6744..7997)
                     /locus_tag="PLQ70_05845"
     CDS             complement(6744..7997)
                     /locus_tag="PLQ70_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HQE34173.1"
                     /translation="MKASIITIGDEILIGQIVDTNSAFIAKSLDRIGIEIHEMISISD
                     SKEHILKTFSKFQNKVDLVIITGGLGPTKDDITKKTFCDYFDDELIVNQEVLKHVTQL
                     IEGFFKRPITQINKDQALVPSKCTVLHNQVGTAPGMWMKKENTVFISLPGVPFEMKYL
                     VENEIIPKVVEEYKRPYILHKTVLTYGIGESLLAETIENWENNLPDFIKLAYLPSLGS
                     VRLRLSARGTNKELLEKAISENVISLSKIIGDSIVGFEEDETLEKIVGRFLTQQGKTI
                     STAESCTGGKIAQVLTSVSGASNYFKGSVVSYATETKVSLLGIHAELIQKHSVVSAEV
                     AQQMAVNVRNIMKTDYAIATTGNAGPSKGESEAEVGAVFIALATPNEVIIEEFNFGQP
                     REKVIDRAVVKSLEMLRKEILKNVQ"
     gene            complement(8131..8475)
                     /locus_tag="PLQ70_05850"
     CDS             complement(8131..8475)
                     /locus_tag="PLQ70_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQE34174.1"
                     /translation="MALHYNLAKVYALSDNDPEFVLQIVTLFVNEIPEDIAEIKEGIK
                     KKDHKHAYSYAHKVKPSLDLLGLKVAFEEILQVEAWTKTEGETKEIKETFKSIKQQVK
                     DAIKELKKDFDL"
     gene            complement(8475..9086)
                     /locus_tag="PLQ70_05855"
     CDS             complement(8475..9086)
                     /locus_tag="PLQ70_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQE34175.1"
                     /translation="MKIICIGRNYASHIAELKNERPSDPVIFLKPDSAILLKQHPFVI
                     PEFSNDIHHEIELIVKINKVGKYIDAKFAHKYYDEISVGIDFTARDLQNELKAKGLPW
                     EKAKAFDGSAVIGEFLPKSQFNSLENITFELTNNNQTVQKGNANQMMWKIDELISYVS
                     QYFTLKIGDIVFTGTPAGVAAVKPEDVLEGFLEGNKLFSIQIK"
BASE COUNT         2914 a         1625 c         1437 g         3137 t
ORIGIN      
        1 cccgaaaaat tcagtcccaa cgtgattatg cgtccgttgt atcaagaagt gattttgccg
       61 aatttatgtt acatcggtgg tggtggagaa attgcgtatt ggttggaatt aaaatctttt
      121 ttcaatgcag tcaacgttac ttttcctgtg cttttactta gaaattcggt tttattagca
      181 accgaaaaac aagtaaaaaa agcagataaa ttaggattgt cttggagtga tttattttcg
      241 aaacaaaccg atttggtcaa tcaaaaaaca gcaacattat ctgaattccc tattgatttt
      301 aggattcaaa aagagcattt aagaaaacaa tttgatacgc ttttagcaat agccaatcaa
      361 accgatgcgt cttttttagg tgcagtaaaa gcacaagaag caaaacaaat aaagggatta
      421 gagaatttag aaaaacgttt gttgaaagct caaaaacgaa agcactctga aactttagaa
      481 cgaattaccg atttacaaaa cgaactgttt ccgaaccaaa gtttacagga acgccaagcc
      541 aatttttcgg aattttatct agaaaacggg aaggatttaa tccataaatt aatcacaacc
      601 ttgaaacctt tggattcaaa atttgaaatc gtaattcttt cctaaaaaat cctttggtaa
      661 atttttaaaa ttgtatagct tagtaatcaa gatataattc caacaaaaac gaagctgatg
      721 actaaagaac ggttgacttc attagatgta tttagaggat taactgtgat aatgatgacc
      781 attgttaata atccgggaag ctgggtagca atttatcccc cattggaaca tgctgagtgg
      841 aatggatgga caccaaccga tttaatattc ccttctttta tttttataat gggaacagct
      901 atcccgtttg caatgcctaa taaaaagttc aactccaccg ttttcaacaa aataatggtg
      961 cgttcattgc gtcttttctg tctagggctt tttctgaatt tctttaacga tctcgaattc
     1021 tttggttggg taggtatgcc attattaatt ggcagactgg caataacttt tgctgtggct
     1081 tatgctttat tggggaagtt tagcctaaaa attaaaaccg ttttagcaat atcagtactc
     1141 acaattttac tctttttagc ctatttcggt atttcagatt atcaagaagt tcgaatccca
     1201 ggtgttttac aacgcatcgg tctggtttat ttttttagtt cgattttgta tttaaaatcc
     1261 aatattagaa ctcaaatttt agtagttaca acgcttttaa ttggttattg ggctttaatg
     1321 acactcgtac cagtccctgg aattggcgcc gcaaatttgg aaaaaggcac taatcttgcg
     1381 gcttacattg acaacctttt cctaaataat catatgtgga gtcgatctaa aacttgggat
     1441 cccgaaggaa tattaattac ccttcccgct atagcttctg gaataatagg aatgttagtg
     1501 gggcaattac tgaatcatca ttggccaaaa attgaaattg tcaaaaaaat gatactaatt
     1561 ggttttagtt tgctaattct tggattacta tgggacatcc tatttcctat caacaaatta
     1621 ctttggagta gttcatttgt tttattcaca tcaggatttg cctctttaag cttaagcttt
     1681 ttatattacg tcatagatat tttgcattat aaaaaaggaa cgaaactatt tctaatttgg
     1741 gggttaaatc caataattgt atttttcttt tcaggaataa ttcctagagt cctgacaatg
     1801 acaaaagtag aacaccccgt atttcctggc aaaccactcg gatttcaaag ctatttttac
     1861 aattacggaa ttgtaccatt gttcgaaaat ccaaaagatg cttcattgac ctacgccttg
     1921 atttatactg ttttttggag tattatttta tgggtgtttt ataaaaacaa attgtttttt
     1981 aaagtatgaa agattagcat aaaacctcaa aaatcgaata tttgcttgcg aattttaagc
     2041 taaaacaaca tcattattcc attaataaac gttacttttg cgcccaacca aaaataaaat
     2101 taaaaaaaat ggcttctaat agaactttta caatgatcaa acccgatgct gtcaaaaacg
     2161 gacacattgg aaacatttta gcaatgatta ctaatgcagg ttacaaaatt gtttctttga
     2221 aattaacaca attaaccgta gccgatgctc aagcctttta tgccgttcac gccgaaagac
     2281 ctttctacgg agaattagta gaatttatgt cgagcggacc aattgttgca gctattcttg
     2341 aaaaagaaaa tgcagttgac gatttcagaa cgttgattgg agcaaccaat ccagctcaag
     2401 ctgcagaagg aactatccgt aaactctacg caacctcaat gggagaaaat gcagttcacg
     2461 gttcagatag tgatgaaaat gcagccatcg aaggagcatt tcattttgct ggaagagagc
     2521 agttttaatc ttagatttct gattttaaag tttagattta aaaaataata caaatagaaa
     2581 atcccgtttc gaatatttga aacgggattt ttttnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnncgtcgc aattcttcgt ttatcatagc aacaattttg gattttccgt
     2761 gggttaattc ttctctcaaa actgccgaag aaagttcaac atacaaagtg cttcctttca
     2821 aaaccacgtt ttttgtataa tgatttacgc catttcccat caaatttttc caagcttctc
     2881 tcacatcaat ttgatccata ccgggttgca atttgttgac ttgaataatc tcgttcaaaa
     2941 tagcaccaat cgtactttga ttatttagtc tttttgccat cactttcaat atttattggg
     3001 gttgcttttt tataatgata ttcttttttt tcagcattca aaaacaccat tttatcgtct
     3061 gaaattgctg ttaactcctc tgaccacttg gcataagaag taacatagtc aaaaaacacc
     3121 ttgtcttttt cgaatcgtac ttttacactt tcttgagtat cattaaccaa aaaagtaccg
     3181 tctaattgag gcatcacttt ttttcgaaag ccactattat ttttgccaat ttgaaaataa
     3241 tcataactat cgtttaccgt ataatctttg gatttaccat cctcaaaaac tactttttca
     3301 atttcccaat aaccatttgt tttagcaata tcctctggtt ttatcttttg ctgacaagag
     3361 ataaaaagaa acgcaacgaa aaaaaatcca attatattct tcataatttt taggagctaa
     3421 ttcctgcttt tcgcttcaat ctttttattt ttttaagaaa aaataaaaag gatttccact
     3481 tcaatcaggg ctagggttta gtggtaaaaa gatgttttaa tctatcgaac aaagttaatc
     3541 ttattagatt gaaagaaaac aaaaataaaa aaaactcatt aaactatttt caatattttt
     3601 ggtcaaattc ctaaaatagc tgaaacatga aaaaaattat ctatagttta ctaatagcta
     3661 tatgctgttt aggatgcagt tcagaaagca caaaacccgt tccatcacca aacgaaacac
     3721 tttattttcc accattatca ggtgatgttt gggaaaccaa atcgattgcc gatttaggat
     3781 ggaaacaagc tgcagttcaa ccacttttgg attatttaga actcaaaaat tccaaaggtt
     3841 ttattatttt ggttaacggt agaattgtta tggagaatta tttcaacgga cattctgcaa
     3901 caaccccctg gtattgggct agtgctggta aaaccttaac cgcaacaatg actggaattg
     3961 cccaacaaga aggtttgctc aacatcaaca acaaagtttc agattatata ggaacgggtt
     4021 ggacgagttt accattagcc aaagaaaact tgattaccaa caaacatttg cttaccatga
     4081 cttcaggtct tgatgatact actgatgata taactcctgc aaaactgatt tataaagccg
     4141 atgcaggtac acgatgggct tatcataacg tttatgtaaa attgcaagat gtaattgcta
     4201 aagcaagcgg acaaacttgg ccaacctatt tcaactcaaa attgagagat aaaatcggta
     4261 tgactggaac ttggatacaa gacggtaaca ataatgtgta ttggagtacc acccgaagta
     4321 tggcgcgttt tggattgcta atgtacaaca agggaaaatg ggaaaacagc actattctta
     4381 acgaaaccta tttcaatgaa gctaccaata cttctcaaaa cattaattta agctatggct
     4441 atttatggtg gttgaatgga aaaacaagtt atcatttacc tcaatcccaa ttgcaattaa
     4501 acggaagcat aattcctagt ggtcccaatg atatgtttat ggctttgggg aaaaatgacc
     4561 aaaaaattta tgtgattcca agcaaaaaaa tggtggtgat aagaatgggt gacgctgccg
     4621 acaatgtaaa tttagcatta tccgattttg acgatgtact ttggcaaaaa atcaatgctt
     4681 tgtaccaata attattctct tttatttctg cttttgaaaa aaccaaaaat aaaaaaacct
     4741 gcacccaaga tcagtaacaa ataatacaaa accatatttt ggtctctaac tagatttgac
     4801 aaaacaaagg caattatact caaaaagtaa agaaaaatag gtggtatatt tttgaaagaa
     4861 gaaaaattca ttataaaact aacattagaa tctttgtcaa aattttgaga tttgacaaag
     4921 atttttttat ttaaaaaaac tatctacaaa ctcatattta ttaaagactt gtaaatcttc
     4981 tataccttct ccaactccaa tatatttcac aggaatttga aattggtcag aaattccaat
     5041 aaccactcca cctttggcgg ttccgtctag ttttgtaacg gcaagcgaag taacttctgt
     5101 agcagcagta aattgtttgg cttgttcaaa agcgttttgt ccagtcgaac catcaataac
     5161 caataaaaca tcgtgtggcg catcgccaac cactttttgc attactcgct ttactttcgt
     5221 taactcattc atcaagttaa ttttgttgtg taaacgacct gcagtatcaa taataacaac
     5281 gtcagcattt tgagctacgg cagattgcaa ggtatcaaac gctacagaag ctggatcgct
     5341 tcccatattt tgtcttacaa ttggaacacc cactctatct gcccaaattt gcaattgatc
     5401 gattgctgcg gctcgaaaag tatcggctgc cccaagtacg actttgaaac ctgctttctt
     5461 aaattgatag gctagttttc caatagtggt tgttttccca acaccattca ctcccactac
     5521 catcaaaaca taaggttttg tggctattgg aattacaaat tcagtagctt caccagaatt
     5581 ggtttcagaa agtaaacccg caatttcttc acgaagaatt tgattgagtt catcagttcc
     5641 aagaaattta tcttccgaaa ctcgtttctc tattcgggtg atgattttaa gagtggtatt
     5701 tactcctaca tccgaggaaa caagaatttc ctctaagtta tctaaaacct catcatcaac
     5761 tttcgatttt ccagcaacgg ccttggttaa ctttgaaaaa aaagaggttt tcgatttctc
     5821 taaacctttg tctaaagatt cctttttttc agaagaaaat aattttttaa aaaaactcat
     5881 cggattattg attgaggatt aacgattttt tatttcagat ttcattgtaa ctcatagcaa
     5941 cttctgaaat ttaattgaca aatataaaaa ataaaaaagc tacttccgaa tgaaagtagc
     6001 ttttctatat agtactatag ttaattattt ctttttcaag aattcatcaa ctgcttcagg
     6061 agccataata gattctacga atgtatatgc acctgttttt ggagatttca ccattttgat
     6121 ggcttttgat aatctcttag aagatgtttg taacgatgct acggttttct ttgccatgat
     6181 tcaatttttt taaaactttt acttcaagta aaaattctta ttatttaatt tctttgtgaa
     6241 cagttacacg tttcaagatt ggattaaatt ttttaatctc taatctgtct ggagtatttt
     6301 ttttgttctt agttgttatg tatcttgaag ttcctgcaac accagttgtt ttgtgttcag
     6361 tacattctaa aattacttgg attctatttc cttttgcttt ctttgccatc ttgatatata
     6421 ggttttagga acggattatt taataaatcc acctgcttga gcttttttca aaactgcagc
     6481 gattccgttt ttattaattg tttttaccgt agatgcagct actcttagag taatccatct
     6541 atcttcttct ggaagataaa aacgcttttt aactaagtta acagaaaatt ttctcttagt
     6601 tttattcata gcgtgagaaa cgttatttcc taccatcgct cttttacctg taaggtcgca
     6661 aactcttgac attatcttat cttttatcgt tattcaaaat cagggtgcaa agaaaagaaa
     6721 aataaaccta ttacgcaaaa aaattattgc acatttttta aaatttcttt tcgcaacatt
     6781 tctaaactct taacaactgc tctatctatc actttttcac ggggttgacc aaaattaaac
     6841 tcttcaatta ttacttcatt tggtgttgcc aatgctataa atactgctcc aacctcggct
     6901 tcagactccc cttttgatgg tccagcgtta cccgttgtag caattgcata atctgttttc
     6961 attatattcc gaacatttac cgccatttgc tgagctactt cagcgcttac aactgaatgt
     7021 ttttgaatta attctgcatg aattcctaaa agtgatacct tagtttctgt tgcataggaa
     7081 actacactac ctttaaaata atttgaagct ccagaaaccg aagtcaaaac ctgtgcaatt
     7141 ttccctcctg tacaactttc ggcagtcgaa atagttttgc cttgttgtgt caaaaatcga
     7201 ccaacaatct tttctagagt ttcgtcttct tcaaaaccta caatgctatc tccaatgatt
     7261 ttagacaaag aaatcacatt ttcggaaatg gctttttcta ataattcttt atttgttcca
     7321 cgtgctgata gtcgcaaacg tacacttcct agacttggta aataggctaa ttttataaaa
     7381 tcgggtaaat tattttccca attttcgatg gtttcggcca ataaactttc gcctattccg
     7441 tatgtcaaaa cggttttatg aagaatgtaa ggtcgtttat attcttcaac aactttgggt
     7501 ataatttcat tctcgaccaa atatttcatt tcaaagggaa ctcctggcag cgaaataaag
     7561 acggtatttt cttttttcat ccacattccc ggtgctgttc cgacttgatt atgaagtacc
     7621 gtacattttg aaggaactaa agcttggtct ttattgattt gagtaattgg ccgcttaaaa
     7681 aaaccttcaa taagttgagt aacgtgtttt aaaacctctt gatttacaat taattcatcg
     7741 tcaaaataat cacaaaaagt ttttttagtg atatcgtctt ttgttgggcc aagtccaccg
     7801 gtaataatga ctaaatcgac tttgttttga aactttgaaa aggtttttag aatgtgttct
     7861 ttactatcac taatagaaat catctcgtgt atttcgatac caattctatc taatgatttg
     7921 gcaataaaag ccgaatttgt atcgacaatt tgtcctatta atatttcgtc accaatggta
     7981 attatagatg ctttcatttg gaagtgagaa gttagaagtg ggaagctatt ttctaaataa
     8041 aaaagagcaa ccagaggaag ctccttttta ttttttttag aaaaaaagta attttaaaaa
     8101 aacttgaaac gtacagcaac aaattgcccc ttatagatcg aagtctttct ttaattcttt
     8161 gatggcatct ttaacttgtt gtttgatgct tttgaaagtt tctttaattt ctttggtttc
     8221 gccttcagtt tttgtccaag cctcgacttg aagaatttct tcgaaagcaa ctttcaatcc
     8281 caataaatct agagatggtt tgactttatg cgcataagaa taggcatgtt tatggtcttt
     8341 ctttttgatt ccttctttga tttctgctat atcttcggga atttcgttaa caaataacgt
     8401 cacgatttgt aaaacaaatt ctggatcgtt gtctgaaagt gcgtacactt ttgctaagtt
     8461 gtaatgtagt gccattattt tatttgtatg ctaaatagtt tatttccttc taaaaatcct
     8521 tctaaaacat cctcgggttt tactgctgca acacctgctg gggttccagt aaaaacaata
     8581 tcgccaattt tcaaggtaaa atactgagac acataagaaa tgagctcgtc aatcttccac
     8641 atcatttggt tggcattccc tttttgaact gtttgattat tattggtcaa ttcaaatgta
     8701 atattttcta atgaattaaa ttgacttttt ggcaaaaatt ctcctatcac agccgagcca
     8761 tcaaatgcct ttgccttttc ccacggcaat ccttttgctt ttaattcgtt ttgcaaatcg
     8821 cgagctgtaa agtcgattcc tacactaatt tcgtcataat atttatgagc aaacttggcg
     8881 tcaatatatt ttccaacttt gtttatttta acaatcagtt caatttcgtg atgaatatcg
     8941 tttgaaaatt caggaatcac aaaaggatgt tgtttcaaca aaatagccga atcgggtttc
     9001 aaaaaaatta cgggatcaga aggtctttcg ttcttcaact ccgcaatgtg actggcataa
     9061 ttacgaccta tacagattat cttcataaaa aagtattcag tggtcagttg cagtggtcag
     9121 tctgtacact gaacaccgat cccaaagctt cgggactgaa cactattatt ttgtatttaa
     9181 ttttctcagt ttaattgccg tcaaaacttt ctt
//
LOCUS       DAOSNN010000085         9155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7527_length_9155_cov_5.604396, whole genome shotgun sequence.
ACCESSION   DAOSNN010000085 DAOSNN010000000
VERSION     DAOSNN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9155
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7527_length_9155_cov_5.604396"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..606
                     /locus_tag="PLQ70_05860"
     CDS             46..606
                     /locus_tag="PLQ70_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQE34176.1"
                     /translation="MLFALFYSCDKKETTVKEKPISDLSIYNLPSKWTNQDGKNIEMK
                     DLKGKVLVMVMIYTSCKSACPRLVADMRNIESRLPQDIKEKVQLVLVSIDPEVDTPNR
                     LKSFSIENKMEGNQWVFLRSTEENTREFAAVLAVNYKKVSPIDFSHSNIISVFNNEGE
                     LVFQQEGLGVNSDATINKITEEAQKD"
     gene            1023..1523
                     /gene="nrfH"
                     /locus_tag="PLQ70_05865"
     CDS             1023..1523
                     /gene="nrfH"
                     /locus_tag="PLQ70_05865"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HQE34177.1"
                     /translation="MLLPYASDDPKACINCHVMNTECATWQVSSHAKRASCNDCHVPH
                     NNVFKKYFFKAKYGLYHSYVYTTRTEPEAIIMHELGQKVVQQNCIRCHDRQLKNPKQS
                     SLMVNYDVHRTDRKCWECHREVPHGRTKSLSTSSMLGKKSEDGDARMPDWLSKQLEKE
                     NNKATK"
     gene            1707..2975
                     /locus_tag="PLQ70_05870"
     CDS             1707..2975
                     /locus_tag="PLQ70_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HQE34178.1"
                     /translation="MKSLKIIYLSLASLFAGSLFAQEFNSDVQLRPRYEYTNGFGTLL
                     TPTTDHTSFIGNRTRLNLNYSDNKLKIKLSLQNVRTWGDAATATVSSKNGVTAFEAWA
                     EYSFTEKWSSKIGRQPLSYDNQRVIGGLDWSNQGRSFDAALIKYKGAKSQLDLGFALN
                     ADSEAKVAPATPYSTDVFKDMQYAWYHTTVKNLGVSVLAMNVGKEYLKTPTEVGLSYF
                     QTFGTYGKYAGAKLGVDYSLYGQTGKIGNNTVSAWEGALNVGYAFSSKFKATAGYEFL
                     SGKDQGATSTMVKSFAPIFGTNHSFNGFMDYFYVGNHANSVGLNDISLKLDFPINKVN
                     VSVAPHFFSAPNKIISGGVEQESYLGTEIDLTASYKLYKDIMLTAGYSQMFATDSMVV
                     LKGGTGLNDTTNNWAYLMVNINPQIFSIKK"
     gene            3108..4583
                     /locus_tag="PLQ70_05875"
     CDS             3108..4583
                     /locus_tag="PLQ70_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34179.1"
                     /translation="MSNLSQSHKILFLNTLAFTICFACWTLNGVLVTFLVDKGIFNWT
                     VVEVGWLLGIPILSGSIFRLPIGIFTDKFGGKIVFSALLLLCSIPLFMLPFADTFWSF
                     AVLSFLFGLVGTSFAVGIGYTSVWYPQNWQGRALGIFGMGNAGAAITTFVAPTLLNGF
                     SKNNPINGWKMLPITYAIVLVIIGLIFIVFAKNKKPLGDSKNIKTLLTPLKSVRVWRF
                     GAYYFLVFGCFVAYSQWLLPNFMNVYQTSLVMGGMFATMFSLPSGIIRAFGGYLSDKY
                     GARKVMYWVLGSSIVISFLLMFPKMEVFTAGPGVLAAKNGVVTEISATEIKIDGKAQK
                     LNTKAVLDDKNTSMLPVRKSWQEVVVQQNQEVKKKELLAKGITQIKFEANMWVYLVLV
                     ILIGISWGIGKAAVFKHIPEYFPNEVGVVGGMVGLIGGLGGFVGPIIFGYLLNSTGLW
                     TSSWIFIFVISVLCLLWMHRTIIKILRVKSPSFTKEIEHNH"
     gene            4658..6250
                     /locus_tag="PLQ70_05880"
     CDS             4658..6250
                     /locus_tag="PLQ70_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34180.1"
                     /translation="MKTWLDKWEPEDEGFWKNTGSKIAWKTLTITTLSLILSFASWFM
                     MSVIAVKLPGLGFPFSKEQLFWLVAIPGLAAGFLRIIHTFILPIFGTRHVVTVATLLK
                     LIPAIGIGFAVMDTNTPFWVFVLLAITSGFGGGDFSSFMPSTSLFFPKRLKGTALGIQ
                     AGIGNFGVSLAQFITPIILSVSIYGTTSVFTSIDVKETKNVLKDSTVEKQKEVFAALD
                     EKSQNKILATVKKPVLDSVTAVTKSNENFEIFSALPLNEKSKAIANANPKLAEKILNK
                     ISPENEAVTNRSIYIQSAAFWYIGFLLVLAFISWFSLKSIPMQASIKEQMDIFKNKHT
                     WYCTITYLMTFGTFAGLSAAFPLMIKYLYGDFPNAPDPLVYAFYGPLIGSASRVAFGF
                     VADKIGGAILTTITGIGILTGAIILVTQGLVSPTSMDQFPLFVGVILTMFFFTGIGNA
                     GTFRQFPIIFSENQRQAAGVIGWTAAIAAFGPFIFSKLIGNNITANGSVNQFFVGLIV
                     FTILATVINWWFYNRKGCEKPS"
     gene            6385..6849
                     /gene="tnpA"
                     /locus_tag="PLQ70_05885"
     CDS             6385..6849
                     /gene="tnpA"
                     /locus_tag="PLQ70_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HQE34181.1"
                     /translation="MANTYTQIHIHFVFAVKYRKGIIHTEWKDDLYKYITGIVQNDNH
                     KLMCINGMSDHIHILIGMRPVQSVSDLMKDVKQGSSLWINENKLAKCHFEWQEGYGAF
                     SYSRSQIQNVVNYIQNQEEHHKKKTFLEEYKEFLEKFEVDFDERYIFKELIE"
     gene            7016..8065
                     /locus_tag="PLQ70_05890"
     CDS             7016..8065
                     /locus_tag="PLQ70_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34182.1"
                     /translation="MKNKTYNTKALLIATGLSIFMIALVFFGSRKLQNFDAALVTYLF
                     GTVFAFFGIVYRYTVWLQRPPTWIYFKRGITYLVTGKVFSHFWFATKETVENIAFQKF
                     IYPRGKYRWVAHFMIAVGCTSAFLITIPLTFGWIHFAMAPNTISTYEAYFFGFKVMDF
                     ELGSIIAFLTFHALNWSSYLVIFGSLYYLRRRLTNPGLIATQSFEGDLLPLILLIAIS
                     VTGLGLTYSYQFMKGFAFDFLAVLHAVTVIMFLIWIPFGKFFHIIQRPAQIGAHIYKK
                     QGMKMGMAVCLHTGEEFATKLHIQDLKTVTEQLGFDFTHEDGTSHLDLSPEGKRSRLA
                     QAHLKARLEGGNLFG"
     gene            8191..>9155
                     /locus_tag="PLQ70_05895"
     CDS             8191..>9155
                     /locus_tag="PLQ70_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQE34183.1"
                     /translation="MAKLPVVEENIVTKFGPHKNYSPKDGYPGRDEPDALVKTHCCFC
                     GMQCGIQLSVKDNKVVGFEPWMEFPFNEGRLCPKGVQRYLQNNHPDRLLSPLKRVEGQ
                     GFVPTSWDDAMSKTVSEIQRIQEKYGNDAFSMLSGVSLTNEKSYLVGKFARVALKTKN
                     LDYNGRLCMVSAGAGNKKAFGLDRASNNYSDLEYAEVIIVAGANISETFPTLTHWIWK
                     ARDRGAKIIVIDPRMIPLARTADIHLDVRPGTDSALYGAMLKYLVDHEMLDHDFIDNY
                     TSGFQETIDAVKDYTLEWAEEVTGIDKNKIREAAELWGKAKTSFLL"
BASE COUNT         2962 a         1381 c         1859 g         2953 t
ORIGIN      
        1 agttcaaata acagaaagta tttgctattt attttgcctt ttttagtgct ttttgcactg
       61 ttttatagct gtgacaaaaa agaaacaaca gtgaaagaaa aacctatttc ggatttgtct
      121 atctacaatt taccttcaaa atggacaaat caagacggca agaatatcga gatgaaggat
      181 ttgaaaggaa aagtactggt aatggttatg atttacactt cttgcaaatc cgcttgccca
      241 agattagtgg ccgatatgcg caatatcgaa agtagattgc ctcaagatat caaggaaaaa
      301 gttcaactcg ttttagtaag tattgatccc gaagttgaca ctcccaatcg attgaaatct
      361 ttctccatcg aaaataaaat ggaaggaaat caatgggttt tcttgcgttc aaccgaagaa
      421 aatacccgag agtttgcggc tgttttggct gtaaattata aaaaagtttc accaatagat
      481 ttctcgcatt ccaatattat aagtgttttt aataacgaag gggaattggt gtttcaacaa
      541 gaaggattgg gtgtaaactc cgatgcaacc atcaacaaaa ttaccgaaga agcgcaaaag
      601 gattgatttt tattttaaat atgagtttaa atcttataat aaaaatggtt cctctttgaa
      661 cttttttttt taaaactcat catttttttt cttttttatt aaaatccgat gcctaatttt
      721 ttaaaaaacc aaagtagttc attaaccttg aattcaaaat ttcatatgtt ctgattttgt
      781 tgattcaaat gtcttttagt atgattcaaa atctttgtgg gtaaaatact agattatgat
      841 ttgagtcata aaaaaaggtt aaatctattt tactttcgca ctaaaataaa gctaataata
      901 aaaattaatt atgaaagcta gatgaattag gcgaatgtac ccgccacccg aatggagacc
      961 tggggtaata ttgctaatag gaatagtgat aggattgggt ctttatatgt taagtattgc
     1021 caatgttgct tccctacgct tcagatgatc caaaagcttg catcaattgt catgttatga
     1081 acaccgaatg tgccacttgg caagtgtctt cacatgctaa aagagctagt tgtaacgatt
     1141 gccatgtgcc acacaacaat gtttttaaaa aatatttctt caaagcaaaa tacggtttgt
     1201 accactccta tgtctatacc actcgaaccg aacccgaagc tatcattatg cacgaactag
     1261 ggcaaaaagt agtacaacaa aactgtattc gctgtcacga ccgacaatta aaaaatccaa
     1321 aacaatccag tttgatggtt aattatgatg tgcatagaac cgatagaaaa tgttgggaat
     1381 gccatcgtga agttccacat ggtagaacca aaagtttatc gacatcatca atgcttggaa
     1441 aaaaatcaga agatggagat gccagaatgc cagattggct atcgaaacaa ttagaaaaag
     1501 aaaataataa agcaactaaa taactataaa atggatccta taaaaaaaag aaacataaga
     1561 aattaggcat ttattattgg tacaatacta attgtatttg tattaggcat attttaataa
     1621 aaatatgatt attatcataa aacgagaaat aatcatatgt taagtttgta caaaattttt
     1681 aaccttttac caaaataagt aattacatga aatctttaaa aataatttat ttatcgctag
     1741 cgagcctttt tgctggtagt ttgtttgcac aggaattcaa ttcggatgtt caactaagac
     1801 ctcgttatga atatactaat gggtttggaa ctcttctgac ccctacaact gatcatacct
     1861 cattcatagg aaatcgtact agattgaact taaattatag tgacaacaaa ttaaaaatta
     1921 aactttcatt acaaaatgtg cgtacttggg gagatgctgc aacggcaacg gtgtctagca
     1981 aaaatggtgt aaccgcattc gaagcttggg cggagtatag ttttactgag aaatggagtt
     2041 ccaaaattgg acgtcaacct ttgtcttatg acaatcaaag ggttatagga gggttggatt
     2101 ggtcaaatca agggcgtagt tttgatgccg ctttgataaa atacaaaggg gcaaaaagcc
     2161 aattggattt aggttttgct ctcaatgcag attctgaagc aaaagtagct ccagcaacac
     2221 cctattcaac agatgtattc aaagacatgc aatatgcgtg gtatcatact actgtcaaaa
     2281 atcttggtgt tagtgtatta gcaatgaatg ttggtaaaga gtacttaaaa acaccaactg
     2341 aagtagggtt aagctatttc caaacttttg gaacgtatgg taaatacgct ggcgcaaaat
     2401 tgggagttga ttacagttta tatggtcaaa caggaaaaat aggcaataac acagtatcgg
     2461 cttgggaagg ggctttgaat gtaggatatg ctttttcttc aaaatttaaa gctacagcgg
     2521 gttatgaatt tttatcagga aaagatcaag gagctacttc tacaatggta aaatcttttg
     2581 ctccaatttt tggtactaac cactctttca atggttttat ggattatttc tatgttggga
     2641 atcacgccaa ttctgtgggt ttaaatgaca tttcattaaa actagatttc ccaatcaata
     2701 aagtaaatgt atcagtagca ccgcactttt tctctgcgcc aaacaaaatt atttctggtg
     2761 gagtagaaca agagtcttac ctaggaactg aaatcgatct gactgcatct tacaaattat
     2821 ataaggatat tatgctaacg gctggttatt cacaaatgtt tgctacagat tctatggttg
     2881 tcttaaaagg tggtacagga ttaaacgata cgacaaacaa ctgggcgtat ttgatggtga
     2941 acataaatcc gcaaattttt tctattaaaa aataattatc taattcaaca aaaagggctg
     3001 tagtaggatg ctctacagct tttttttatt tattatttta aaaaaagaca tttttgtcca
     3061 ttataaaaag taatttaagt gtcctttcag aatttaaaat ttcaagtatg agtaatttat
     3121 cacaatccca caaaatatta tttctaaaca cattggcttt tacaatttgt ttcgcttgtt
     3181 ggactttgaa tggtgttttg gtcacttttt tggtcgataa aggaattttt aattggacgg
     3241 ttgttgaggt aggatggttg ttaggaattc caattttatc tggatccatt tttagattgc
     3301 caatagggat tttcacggac aaatttggag gtaaaatagt gttttcggct ctgttattac
     3361 tttgctctat tccccttttt atgttgcctt tcgctgatac tttttggagt tttgccgtgt
     3421 tgagttttct ttttggattg gtaggaacca gttttgcagt tggaatcgga tatacctcag
     3481 tttggtatcc ccaaaattgg caaggaagag cattgggaat tttcggaatg ggaaatgcag
     3541 gagctgccat caccactttt gtggcgccaa cattattaaa tggtttctct aaaaataatc
     3601 ccattaatgg ttggaaaatg ttacccataa cttatgctat tgttttagtc ataataggtc
     3661 ttatctttat agtttttgct aaaaataaaa aacctcttgg agattccaaa aatatcaaaa
     3721 cacttttaac accactgaaa tcggttaggg tttggcgttt tggagcctac tatttcttag
     3781 tttttggttg ttttgtggct tactctcaat ggttattgcc taattttatg aatgtatatc
     3841 aaacctcgct cgttatggga ggaatgtttg ctacaatgtt cagtttgcca tcgggtatta
     3901 ttagagcttt tggtggctat ttatcggata aatatggggc tcgaaaagta atgtattggg
     3961 ttttgggttc ttcgatagtc atcagctttt tattgatgtt ccctaaaatg gaagtcttta
     4021 ctgctggtcc tggagttttg gctgcaaaaa acggcgtggt taccgaaatc tctgctaccg
     4081 aaattaaaat tgatggaaaa gcacaaaagt taaatacaaa agcggttttg gatgataaaa
     4141 atacatcaat gcttcccgtt agaaagtcgt ggcaagaagt tgttgttcag caaaatcaag
     4201 aagtgaaaaa gaaagaattg cttgccaaag gaatcactca aattaagttc gaagccaata
     4261 tgtgggtgta tttggtacta gtaattctaa taggaatttc ttggggaatc ggaaaagcag
     4321 ccgtgttcaa acacattcca gaatattttc cgaatgaagt tggagttgta ggtggaatgg
     4381 ttggattaat cggtggatta ggtggatttg tggggccaat tatctttgga tatttattaa
     4441 attctactgg attatggacg agttcttgga ttttcatttt tgtaatatcc gtgctttgtc
     4501 tgctttggat gcaccgcacg attataaaaa ttttaagagt aaaatcaccg agttttacca
     4561 aggagattga acacaatcat taaatcaaat ttcaattgca atagcaatga caattgcaat
     4621 acaatatcaa taaaaaaata attaaaacag aaatagtatg aaaacttggc tagacaaatg
     4681 ggaaccggaa gatgaaggat tttggaaaaa tacaggaagt aaaattgctt ggaagacatt
     4741 gacaattaca actttgtcat tgatattgtc ttttgcctct tggtttatga tgagcgtaat
     4801 agcagtgaaa ttaccagggt taggtttccc tttttctaaa gagcagcttt tttggttggt
     4861 ggctatacct ggtttggcag caggattttt aagaattata cacaccttta ttctgcctat
     4921 ttttggaaca aggcatgtag ttactgtggc aactttactc aagttaattc cagcaattgg
     4981 aattgggttt gcggtaatgg ataccaatac tccattttgg gtatttgttc ttttggctat
     5041 taccagtggt tttggtggtg gagacttttc atcctttatg cctagcacaa gtctgttttt
     5101 tccaaaaaga ttaaaaggaa ctgcattggg aatccaagcg ggaattggga attttggagt
     5161 tagtttagct cagtttatta ccccgattat tttgagtgtt agtatttacg ggactacttc
     5221 ggtatttaca agcatcgatg ttaaggaaac aaagaatgtt ttaaaagatt cgacagtaga
     5281 gaaacaaaaa gaagtttttg cggccttgga tgaaaaatca caaaataaaa ttctggctac
     5341 tgtaaagaaa ccagtattag attcggttac agcggtaacg aaatcaaatg aaaatttcga
     5401 aattttttct gcattgccac tcaatgagaa atctaaagcg attgcaaatg caaatcctaa
     5461 attggccgaa aaaatattaa acaaaattag tcccgaaaat gaggctgtta caaacaggag
     5521 catctatatt caatctgctg cattttggta cattggtttt cttttggttt tggcatttat
     5581 cagttggttt agtttgaaaa gtattccgat gcaagcttcg ataaaagagc aaatggatat
     5641 ttttaaaaac aagcatactt ggtattgcac cattacttat ctgatgactt ttggaacttt
     5701 tgcaggatta tcagcagctt ttcctttgat gataaaatat ttgtatggtg attttccaaa
     5761 tgcgccggat ccattggtgt atgctttcta cgggccgctg attggttctg ccagcagagt
     5821 cgcttttggt tttgtagccg ataaaattgg tggagcaatt ttaacgacca taaccggaat
     5881 cggaattttg actggagcta taattttagt gacacaagga ttggtttctc caaccagtat
     5941 ggatcagttt ccgttgtttg tgggggtgat tttgacgatg tttttcttca caggaattgg
     6001 taatgcgggc acttttagac agtttccaat cattttttca gagaatcaaa gacaagcggc
     6061 aggtgtaatt ggttggacag cagccatcgc agcttttggc ccttttatct tttcaaaatt
     6121 aattggaaat aacattacgg caaatggatc ggttaatcaa ttttttgttg gtttgattgt
     6181 tttcactatc ttagcaactg ttattaattg gtggttttac aaccgtaaag gatgtgaaaa
     6241 accgagttag ttcgacactc tcagaggcga ttaaaaatag gttttaaaag aataatacgt
     6301 ttatttttat gtgccgttag gcactgtata ttggtagaaa aaaaatgtga ataaagaaaa
     6361 atgtcccgta gggactatac aattatggct aatacttata cacagattca tattcatttt
     6421 gtttttgcag taaaatacag aaaaggaatc attcatacag aatggaaaga tgatttgtat
     6481 aaatacatta ctggtattgt tcaaaatgat aatcacaaac ttatgtgtat aaatggtatg
     6541 tcagatcata ttcatatttt aattgggatg aggcctgtac aatctgtttc agatttaatg
     6601 aaagatgtaa agcaaggttc ttcgctttgg attaatgaaa ataaacttgc aaaatgtcat
     6661 tttgaatggc aggaaggata tggtgcattt tcatatagta gatctcagat tcaaaatgtt
     6721 gtaaattata ttcagaatca agaagaacat cataaaaaga aaacattttt agaagaatac
     6781 aaagagtttc ttgagaaatt tgaagtcgat tttgatgaaa gatatatttt taaagagtta
     6841 atagagtagg gtatagtcac tactggacaa aggtgagacg atgatttctt tttctaccaa
     6901 tatttagttc ctagcggaac agagaatata aaatgatata acctaaattt caatgatttc
     6961 aagatttagt gcaaaaatga agaagccgaa tttcgtaaaa aaataatatt aaattatgaa
     7021 aaacaaaacg tataacacca aagcgctact aatcgcaaca ggactttcta tttttatgat
     7081 tgcccttgtg ttttttggtt ctagaaaatt acaaaatttt gatgcggcat tagtaactta
     7141 tttattcggg acagtatttg ctttctttgg tattgtatat aggtacacag tttggttgca
     7201 aagaccgccc acttggattt atttcaagag aggaattacc tatttagtaa cgggaaaagt
     7261 attttcgcat ttttggtttg ctaccaaaga aacggtggag aatattgcgt tccaaaaatt
     7321 tatttatcca aggggaaaat accgttgggt ggcgcatttt atgattgcgg tgggttgtac
     7381 ttcggcattt ttgataacaa ttccacttac ttttggttgg attcactttg cgatggctcc
     7441 caatacgatt tctacttatg aagcctactt ttttgggttt aaagtaatgg attttgaact
     7501 gggttcaata attgcttttc ttacttttca cgccttgaac tggtcttcgt acttggttat
     7561 ttttggttca ctctattatt taagaagacg tttgacgaat cctggattga ttgccacaca
     7621 atcttttgaa ggagatttgc tgcctttaat attgttgatt gccatttccg taacaggatt
     7681 gggattgacg tattcgtacc aatttatgaa aggatttgcg tttgatttct tggcggtttt
     7741 acacgcagtt acggtgatta tgtttttgat ttggattccg ttcgggaagt tttttcatat
     7801 cattcagcgt ccagctcaaa tcggggcgca tatatacaaa aagcaaggaa tgaaaatggg
     7861 aatggcggtt tgtctgcata cgggagaaga gtttgccaca aaattacata ttcaggattt
     7921 gaaaacagta acggaacaat tgggtttcga ttttacgcac gaagacggaa catcacactt
     7981 ggatttgagt cctgaaggga aaagatctcg attggcacaa gcgcatttga aagccagact
     8041 ggaaggagga aatttgtttg gataaaaaca caccgcaaag acgccaaggc acaaagaaaa
     8101 ataattcatc ggtaaagaaa aaatatactg taaagaacaa agagacaaag aaataaaatt
     8161 gcgacttagc ggtaaaaaca aaacaaaaaa atggcaaaat taccagtagt agaggagaat
     8221 attgtaacga agttcggacc tcacaagaat tatagtccaa aagatggtta tccaggaaga
     8281 gatgaacctg atgcgttggt aaaaacacat tgttgttttt gcggaatgca atgtggtatt
     8341 caattgtcgg tcaaagacaa taaagtggtg ggttttgaac cttggatgga atttcctttt
     8401 aatgaaggaa gattgtgtcc aaaaggagtg cagcgttatt tgcaaaacaa ccacccagac
     8461 cgacttttgt caccattaaa aagagtggaa ggacaaggat ttgtgcctac ttcttgggac
     8521 gatgcaatga gtaaaacggt ttcggaaatt caacgcattc aggaaaaata tggaaatgat
     8581 gctttttcaa tgctttctgg cgtttcgttg acgaatgaaa aaagttattt ggttggaaag
     8641 tttgcgcgtg tggccttgaa aaccaaaaat ctggattaca acgggcgttt gtgtatggtg
     8701 agtgctggtg caggaaacaa aaaagcgttt ggactggaca gggcttcgaa taattattcg
     8761 gatttggaat atgccgaagt gattattgtg gctggtgcca atatcagcga aacttttcca
     8821 acattgaccc actggatttg gaaagcccgt gaccgtggtg ccaaaattat tgtgattgac
     8881 ccaaggatga ttccgttggc gcgaactgcc gatattcatt tggatgtgcg tccgggaacc
     8941 gactcggctt tgtacggagc gatgctgaaa tacttggtgg accacgaaat gctggaccac
     9001 gattttatcg ataattatac ttctggtttt caggaaacga ttgatgccgt aaaagattac
     9061 accctagaat gggcggagga agtgacagga atagacaaaa ataaaatacg cgaagccgcc
     9121 gaattatggg gcaaagccaa aacgagtttc ttgct
//
LOCUS       DAOSNN010000086         9118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7568_length_9118_cov_4.994042, whole genome shotgun sequence.
ACCESSION   DAOSNN010000086 DAOSNN010000000
VERSION     DAOSNN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7568_length_9118_cov_4.994042"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLQ70_05900"
     CDS             <1..701
                     /locus_tag="PLQ70_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQE34184.1"
                     /translation="REFLGNVSHELKTPLFTVQGYLSTLLDGAMEDKNIRKKYLKRAE
                     KGVERLIYIVEDLDMITKLEVGDLNLEFSEFDIVELIQNVFDLLEMKADKKKITLAFE
                     NYHIKPVFVKGDKDKLQQLVENLIVNSIKYGNEGGLTEVSIINLSKKKVLVRITDNGE
                     GIEQQNLPRIFERFYRVDKSGARTEGGSGLGLAIVKHIVEAHKEKIYVESEYGIGSEF
                     SFTLTKARLNIKTN"
     gene            906..2306
                     /locus_tag="PLQ70_05905"
     CDS             906..2306
                     /locus_tag="PLQ70_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34185.1"
                     /translation="MKKILFASLLFVSFATQAQDVKKDEIKKEVVRILDSINKSKASE
                     EGLTNVTSNDSLKEKVESHTMKFESMDEQVSTMQSTLDKLSKIKISGYIQAQYETYDT
                     WNSGIHGISPITGAPASTNTFSLRRARVKFTYEALDGVTFVLCPNFEVDKVRLTDVYA
                     QLNDRWMQTFSLWVGLFNRPTYEVEFSSSSREFSERSLVTRTLYPQERDMGAKLEANF
                     LTKFEIPLKLQFAILNGNFGIGTAANQVKDIDNTKDFMVRAVYSLKFPSKGLGIDFGG
                     HGYFGHNTIITQTATPAKFTDENGTVFTPKVGDDLKKDWFGAEMQVYYDILGGASLKA
                     EYIAGSMTGFKATDFPAQVNPLFTSNNKVRNIEGHYISLIKNLGKDHMLSVRYDVFDP
                     NTKKSGNEVATIDDLKFQNWTFAYQYFFNENIKIMLSYLMPINEKSANSGITNPDWAN
                     VDHKDNSLTIRFQTRF"
     gene            2496..3329
                     /locus_tag="PLQ70_05910"
     CDS             2496..3329
                     /locus_tag="PLQ70_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQE34186.1"
                     /translation="MKTTNFKIAALLVAVMTIGFSFTTINKVTVKGSDTMVILSQKWA
                     EVYMKKNPGTSIQVTGGGSGVGLAALINGSTDIANSSRPIKAAEVEKLKARYNTMGVE
                     IACAKDGLSVYLNKANPVASLTIKQIGQIFAGKITNWKDVGGPDAAIKLYGRESSSGT
                     FGFFKDNVVKTDFSPTCQTLPGTAAIVNAVKKDKFSIGYGGAAYAEGVKDCAVKKDDK
                     SPAILPTAATIKNHTYPITRYLYMYLKSKPTGETKAFIDWILSPAGQGLIEEVGYYPL
                     K"
     gene            3412..4647
                     /gene="pstC"
                     /locus_tag="PLQ70_05915"
     CDS             3412..4647
                     /gene="pstC"
                     /locus_tag="PLQ70_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQE34187.1"
                     /translation="MNSQLPIQQTFTKESLKKQFRLSEFLAEKIIKSVAFMSIAIIFL
                     IFFFVFKESLPLFSFGKDTQAKTEVATANKPESYGATTEDLKPETYGAETSTEELKPE
                     TYGAPTEELKPETYGSEPVSNEDLKPESYGDTPKEDLSVPSSDEPIDAVSQNEEGSDK
                     TWSTFFSTEWVPVSDNPRFGLIGLLVGTLKVTIIAMLFAGPLAILAAVYTSCFASNRR
                     KEIIKPIIEMLAAFPSVVIGFFAMMVLATFFQNIFGYDSRLNAFIGGVAMALAAIPII
                     YTISEDALSAVPKTYTEASLALGASKAQTAFSVILPAATPGIFAALLLGVGRVFGETM
                     IALMATGNAALLSANPFESVRTFAATIGSEMAETVFGETHYSVLFLIGSLLFIFSFIL
                     NAVAEFYVKGKLIKKFQGK"
     gene            4649..5596
                     /gene="pstA"
                     /locus_tag="PLQ70_05920"
     CDS             4649..5596
                     /gene="pstA"
                     /locus_tag="PLQ70_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQE34188.1"
                     /translation="MENTMDIIENPFSKANNNKGKEIKEKAVVGITQIAVLLIIAILF
                     VILGIIIYQGRAKFSWEFISSFPTEGMTKGGIFPALIGTFILVIVMSIAAVPFGTITA
                     IYLTEYAHEKSKFAAAVRFSIRTLAVVPSIIFGLFGLGFFIQFVGTGMDKAFNGGELH
                     WGQPNILWASLTMALLTLPVIIVSVEEALKTIPRELREASLALGATKWQTIKQVVFPG
                     SISGIMTGTILAVSRGAGEVAPILFTGAAYYLATLPGSLSDQFMNLGYHVYIMSTQSS
                     DVEKTMPIQFATTLVLLILTLSLNLVAVVIRSRIRRKAK"
     gene            5671..6429
                     /gene="pstB"
                     /locus_tag="PLQ70_05925"
     CDS             5671..6429
                     /gene="pstB"
                     /locus_tag="PLQ70_05925"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQE34189.1"
                     /translation="MRDIKIQVNDLSLYYGEKKALKEISMQIPANKVTALIGPSGCGK
                     STFLRCINRMNDLIPSVKITGEMLVEGVDIYDKNVDVVNIRKKIGMVFQKSNPFPKSI
                     YENVAYGPRINGLKDKTQLDEIVETSLRQAAIWDEFKDRLDDSALGLSGGQQQRLCIA
                     RTLAVSPDIILMDEPASALDPISTSKIEELVHQLKENYTIIIVTHNMQQAARTSDHTA
                     FFYMGELIEMGKTNTIFTKPEKKQTEDYITGRFG"
     gene            6512..7189
                     /gene="phoU"
                     /locus_tag="PLQ70_05930"
     CDS             6512..7189
                     /gene="phoU"
                     /locus_tag="PLQ70_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQE34190.1"
                     /translation="MTHFEMELEKLKNVIIKIGTLAVSQVGESVKALLFEPIEGKEVK
                     KTEIKIDKLDVKIDEICQSIFALQQPVASDLRFIMASMQISNEIERIGDLAVSVIKRA
                     KNIKEKHDLITKFEIGDLAKEVELITAKTNECLLTLDEKTIAEILTLNKNIKTKGDEI
                     IDHIIDEMKSHSKVVVSGTNLVIALKHLERISDHCSNVAEYVYFMVNAKIIKHEKHDD
                     LNINLDK"
     gene            complement(7268..7999)
                     /locus_tag="PLQ70_05935"
     CDS             complement(7268..7999)
                     /locus_tag="PLQ70_05935"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HQE34191.1"
                     /translation="MSLLSLSEKSIKTIKSITNSTYLDVIGLLIVVSASFALDYHKTV
                     FNFTFQHQKYVFYLGYISILNTGFSMMGNRLVTKKNNIGNFITTCNTFLSGSIDYLLG
                     NAGAILTYPVSFIGNYIVFNTWKKSRELRSIDFIFYRNIVLGFLFSFVLNYIAFQYLS
                     DKPIDWKLFFAITIPAGLTFGGTFNTARMYPDNWFLWQFYNLTKAIQNLMLMNIANVA
                     KYTFYFFNAIIGYITWKDDKKKGNI"
     gene            complement(8068..>9118)
                     /locus_tag="PLQ70_05940"
     CDS             complement(8068..>9118)
                     /locus_tag="PLQ70_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HQE34192.1"
                     /translation="PKQLGDILFDKLKIGGAKQKKTKTGQYATGEEILSYLANDNEIV
                     RDILEWRQMVKLQSTYIDALPNQVDKKTGRVHTDYMQTVAATGRLSSNNPNLQNIPIR
                     TERGRLIRKAFIARDENYTLVSADYSQIELRIIAALSGEENMIKAFQNNEDIHKSTAA
                     KVFNVPLEEVTKEQRSNAKTVNFGIIYGVSAFGLSNQTSLSRKESAELIDAYYATYPK
                     LKSYMSNQVDFARKHGYVQTVLGRRRYLKDINSANMMVKSGAERNAVNAPIQGSAADI
                     IKIAMINIHKKLTSGNWKSKMLLQVHDELVFDVHNSELEKIQPMIKHEMENAFIMDVP
                     LDVEIGAGKNWLEAH"
BASE COUNT         3126 a         1378 c         1671 g         2943 t
ORIGIN      
        1 gaagagaatt tttaggcaac gtatctcacg aacttaaaac ccctttattt acagtgcaag
       61 gctatctatc aacacttctt gatggcgcaa tggaagacaa aaacattcga aaaaaatacc
      121 taaaacgtgc cgaaaaaggt gtagagcgat taatctatat tgtcgaagat ttagatatga
      181 ttaccaaact tgaagtaggt gatttgaatc tagaattttc tgaatttgat atagtagaat
      241 tgattcaaaa cgttttcgac ttattagaaa tgaaagctga taagaagaaa atcactttgg
      301 ctttcgaaaa ttatcacatt aaacccgttt ttgtgaaagg ggataaagac aaattgcagc
      361 aacttgtaga gaatttgatt gtcaactcca taaaatatgg taatgaaggt gggctaaccg
      421 aagtgagtat catcaatttg agtaagaaaa aagttttagt ccgaattaca gataatggtg
      481 aaggaataga acaacaaaat cttccaagaa tttttgaacg tttttatcga gtggataaaa
      541 gtggagctcg aacagaaggc ggttcaggat tgggattggc aattgtaaaa cacattgtcg
      601 aagctcataa agaaaagata tatgtagaaa gtgaatatgg aattggctca gaattttctt
      661 ttactttgac aaaagctagg ctaaatatca aaacaaatta agagatttaa ttaacaaatg
      721 gataatctaa tttttgatga ttaaataatt gaaaaacaaa cagttgtatt ttatttttga
      781 ttgtaccctt caagttttac attaatatat cgtcaaatca tcattaattt atcatcaata
      841 taatgttatg ttaacattag cttaacattg taatctacct ttgcaaaaaa attaaatcat
      901 aaaaaatgaa aaagatttta tttgcaagtt tactttttgt ttcatttgca actcaagcac
      961 aagatgtcaa aaaagacgaa atcaaaaaag aagtagttcg tattttagac tctattaata
     1021 agtcaaaagc ttctgaagaa ggattgacta atgtaacaag taatgattct ttgaaagaaa
     1081 aagttgagag tcatactatg aaatttgaaa gtatggatga gcaagtttcg actatgcaat
     1141 ctactttaga taaactatca aaaattaaaa tctctggata tattcaagca caatatgaaa
     1201 cttatgacac ttggaattct ggaattcatg gtataagtcc aataactgga gcgccagctt
     1261 ctacaaatac attttcttta agaagagctc gtgttaaatt tacttacgaa gcattagacg
     1321 gtgttacatt tgtattgtgt cctaactttg aggttgataa agtgcgactt acagatgttt
     1381 atgctcaatt gaatgatcgc tggatgcaaa ctttctcgtt atgggttggg ttatttaaca
     1441 gacccactta tgaggtagaa ttttcatcat catcgcgaga attttcagaa agaagtttgg
     1501 taactagaac attgtatcct caagaaagag atatgggggc aaaattagaa gcaaattttt
     1561 taactaaatt tgaaattcct ttgaaacttc aattcgcaat attgaatggt aattttggaa
     1621 taggaacagc agcaaatcaa gtaaaagata tcgacaatac taaagatttt atggtaagag
     1681 ctgtttactc attaaaattt cctagcaaag gactgggtat tgattttgga ggacacggat
     1741 attttggtca caatactata attactcaaa cagctactcc agctaagttt acagatgaaa
     1801 atggtactgt atttactcct aaagttggtg atgatctaaa aaaagattgg tttggagctg
     1861 aaatgcaagt gtattacgat attcttggtg gagcttctct aaaagcagaa tacattgcag
     1921 gttcaatgac aggtttcaaa gcaactgatt ttccagcaca agtgaatcca ttatttacgt
     1981 caaataataa agtaagaaat atcgaaggac attacatttc attaattaaa aatttaggaa
     2041 aagatcatat gttatctgtg agatatgatg tttttgatcc taatacaaaa aaatcaggga
     2101 atgaagttgc tactatcgat gatttaaaat tccaaaattg gacttttgct tatcaatatt
     2161 tctttaacga aaacataaaa ataatgttga gttatttgat gccaataaat gaaaaaagtg
     2221 ctaactcagg aattactaat ccagattggg caaatgtgga tcacaaagac aattcattaa
     2281 caataagatt tcaaacaaga ttctaattta gaatttttta tttaacataa gaaattgata
     2341 atcagtgttt tttttcttgt tctggttatg aaattttttc tttattgtaa aatttgagta
     2401 aagaatacct aaatagtaaa gattgaataa tactaactta acatagactt aacgttgcgg
     2461 ctttactttt gtcaaaaaat taaaagatat taaaaatgaa aacaacaaat tttaaaatag
     2521 cagccctttt ggtagcggtg atgactattg gtttttcttt tactacaatc aataaagtaa
     2581 ccgttaaagg ttctgatacg atggttattt tgtctcaaaa atgggcagaa gtttatatga
     2641 agaaaaatcc agggacttct attcaagtta ctggtggagg atctggtgtg gggcttgcag
     2701 ctttaatcaa tggttctact gatattgcca attctagtcg tccaatcaaa gctgctgaag
     2761 ttgaaaaatt gaaagcaaga tacaatacaa tgggagttga aattgcttgt gcaaaagacg
     2821 gtttgtctgt gtatttgaat aaagctaatc cagttgcaag tttaacaatt aaacaaattg
     2881 gacaaatttt cgctggtaaa attaccaatt ggaaagatgt aggtggtcca gatgctgcta
     2941 tcaaattata cggaagagaa agtagttcag gaacttttgg tttctttaaa gacaacgtag
     3001 tgaaaacaga tttttctcca acttgtcaaa cattgcctgg aacagcggca atcgtaaatg
     3061 ctgtaaaaaa agataaattt tcaattggtt acggtggagc agcttatgct gaaggggtga
     3121 aagattgcgc agtgaagaaa gacgataaaa gtcctgcaat tttgccaaca gctgctacaa
     3181 tcaaaaatca tacttaccca atcacaaggt atttgtatat gtatttgaaa tcaaaaccta
     3241 caggtgaaac caaagcattt attgattgga ttttgagtcc agcaggacaa ggtcttattg
     3301 aagaagtggg ttattatcct ttaaaataat agaaatattt aatatccctc attttttttg
     3361 aagtgaggga tatttgtgtt tatatctaaa cgaattccta aataaataag gatgaattct
     3421 caattgccaa tccaacaaac ttttacaaaa gaaagtttaa aaaaacaatt caggctttct
     3481 gaatttttag ccgaaaaaat tataaagtcg gttgctttta tgtcgatcgc aatcattttt
     3541 ttgattttct tttttgtttt caaagaatct ttacctttgt ttagttttgg aaaagatacg
     3601 caagcaaaaa cagaagttgc aacggctaat aaacctgaat cctatggtgc aacaaccgag
     3661 gatttaaaac ccgaaaccta tggtgctgaa acttctactg aagagttaaa gcctgaaacc
     3721 tatggtgctc ctacagaaga attgaagcca gagacgtatg gttcagaacc tgtatctaat
     3781 gaagatttaa aaccagaaag ttacggagat actcctaaag aagatttatc tgttccttca
     3841 tcagacgaac ctatagatgc cgtttcccaa aatgaagaag gatctgacaa gacttggagt
     3901 acatttttta gtaccgagtg ggttccagtt tccgataatc ctcgttttgg attgattggc
     3961 ttacttgttg ggactttaaa agtaactata attgcgatgc tttttgctgg acctttggca
     4021 atattagcag ctgtttatac ttcttgtttt gcatccaata gaagaaaaga aatcattaag
     4081 cctatcatag aaatgctagc ggcttttcca tctgtagtga ttggtttttt tgcaatgatg
     4141 gttttggcta ctttttttca aaatattttt ggctatgatt ctcgactgaa tgcttttatt
     4201 ggaggtgttg cgatggcatt ggctgcgatt ccaattattt ataccatttc agaagatgct
     4261 ttatcggcag ttcctaaaac ctataccgaa gccagtttgg cattaggagc cagcaaagct
     4321 caaaccgctt tttcggtgat tttgccagca gctactccag gaatttttgc agccttactt
     4381 ttaggtgtgg ggcgtgtttt tggcgaaaca atgattgctt taatggcaac aggaaacgcc
     4441 gctttattgt cggcaaatcc atttgaaagt gtgcgaactt ttgctgcaac tattggttct
     4501 gaaatggccg aaacagtttt tggcgaaacc cattatagcg ttttgttttt aattggttct
     4561 ttacttttta tattttcttt cattttaaat gcagtggcag aattttatgt taaaggaaaa
     4621 ttaatcaaga aattccaagg taaataatat ggaaaataca atggatataa tagaaaatcc
     4681 gttttctaaa gcgaataata acaaaggcaa agaaattaaa gaaaaagcag ttgtgggaat
     4741 tactcaaatt gcagttcttt taattattgc catcttattt gttatcttgg gaattataat
     4801 ttatcaagga cgtgcaaagt tttcttggga atttattagt tccttcccaa ccgaaggtat
     4861 gactaaagga ggaatatttc ctgccttgat aggaactttt atattagtga ttgtaatgtc
     4921 tattgcagca gttcctttcg gtacaattac agcaatttac ttaacagaat atgcacacga
     4981 aaaatcaaaa tttgcagcag cagtacgatt ttctattcgc actctggcag ttgttccttc
     5041 tataattttt ggactttttg gattgggatt tttcatccaa tttgtgggaa caggaatgga
     5101 taaggctttc aatggaggcg aattgcattg gggacaaccc aatattcttt gggcgagttt
     5161 aacaatggcg ttattgactt tacctgtgat tatagtttca gtagaagaag ccttgaaaac
     5221 aattccaaga gaattgcgag aagcgagttt agcattggga gcaaccaaat ggcaaacgat
     5281 aaaacaagtc gtttttccgg gttctatttc tggaattatg acaggaacaa ttcttgcagt
     5341 gagtagaggt gctggcgaag tagcccctat tttatttaca ggagcagctt attatttggc
     5401 cacattgcca ggttctttga gcgaccaatt tatgaatttg ggctatcacg tttatattat
     5461 gtcaacccaa tcttcggatg tcgagaaaac aatgcctatt caatttgcaa ctacattggt
     5521 gttgttgatt ttaacattat cattgaattt agttgcagta gttattagat ccagaattag
     5581 aagaaaagca aaataaagat tgcagatttc agattaacga tttttgattg cagattaaaa
     5641 atcgtatttt ctatcaaata aaaatataaa atgagagata taaaaataca ggttaacgat
     5701 ttatctttat actacggcga aaagaaagca ttaaaagaaa tttcgatgca gattcctgcc
     5761 aataaggtca ctgctttaat cgggccttcg ggttgtggta agtcaacctt tttgagatgc
     5821 attaaccgaa tgaatgattt gattccgagt gtaaaaatca ccggcgaaat gctagttgaa
     5881 ggcgttgata tttacgataa aaatgtcgat gtggttaaca ttcgaaaaaa aattggaatg
     5941 gttttccaaa aatcgaatcc gtttccgaaa tctatttatg aaaatgtggc ttatggccca
     6001 agaattaatg gattgaaaga caaaactcaa ttagacgaaa tcgtagaaac ttctttgcgc
     6061 caagctgcta tttgggatga atttaaagat cgattagatg attcagcttt gggattatcg
     6121 ggtggtcaac aacaacgttt gtgcattgct cgaacattgg cagtaagccc agatattatt
     6181 ttgatggacg aaccagccag tgctttggat ccaatttcta cctcaaaaat tgaagaattg
     6241 gtgcatcaat taaaagaaaa ttacaccatt attattgtta ctcataatat gcaacaagct
     6301 gcgagaacta gcgatcatac cgcatttttt tatatgggag aattgattga aatgggtaaa
     6361 acaaatacta tatttaccaa accagaaaag aaacaaaccg aggattatat cacgggaaga
     6421 tttggataat ggtttaaatt ggttaatcat ttaattgttt taaccaatta actaattgac
     6481 cgattgacct ttttaaacta aaaattgtaa gatgacacat tttgaaatgg aattagaaaa
     6541 actaaaaaac gtaattataa aaattgggac tcttgctgtt agccaagttg gcgaatcggt
     6601 taaagccctt ttgtttgaac ctattgaagg gaaagaagtc aagaaaaccg aaataaaaat
     6661 tgataaatta gatgtgaaaa tcgacgaaat ttgtcaaagt atttttgctt tgcaacaacc
     6721 agtagcttct gatttgcgtt ttattatggc ttccatgcaa ataagtaacg agattgaaag
     6781 aattggtgat ttggcggtga gtgttataaa aagagccaaa aacattaagg aaaagcacga
     6841 tttaatcaca aagtttgaaa taggagattt ggccaaagaa gttgaattga ttactgcaaa
     6901 aactaatgag tgcctattga cactagacga aaaaacaatc gccgaaattt taactttgaa
     6961 taaaaatatc aaaactaaag gcgatgaaat aatcgaccac attattgatg aaatgaaatc
     7021 ccattctaaa gtagtggttt caggaactaa tttggttata gcattaaaac atttagagcg
     7081 catctcagat cattgttcta atgtggccga atatgtttat tttatggtga atgctaaaat
     7141 tattaaacac gaaaaacacg atgatttgaa tataaattta gataaataat ctaatatttc
     7201 tcttgaaacc gcccatttcc atcttaatta ttttgatgca aatgggcggt ttcatttttt
     7261 gaaaagatta aatatttcct tttttcttat cgtctttcca agtaatgtag cctataatgg
     7321 cattgaagaa gtagaaagta tatttggcaa cattagcaat attcatgagc atcaggttct
     7381 gaatggcttt tgttaaattg taaaattgcc acagaaacca attgtcagga tacattctag
     7441 ccgtgttgaa ggtcccgcca aacgttaatc ctgctggaat agtaatggca aaaaacaact
     7501 tccaatcaat gggtttatca gataaatatt ggaaggcgat gtagtttaaa acgaaagaaa
     7561 ataaaaatcc taaaactata tttctgtaaa atataaaatc tattgaacgc aattctctgc
     7621 tttttttcca agtgttaaaa acaatataat ttccaataaa cgatacagga taagtaagaa
     7681 ttgctccggc gtttccaagt aaataatcaa ttgaaccgct tagaaaagta ttgcaagtgg
     7741 tgataaaatt gccaatatta tttttcttgg tcaccaatcg atttcccatc atcgaaaaac
     7801 cagtattcag aatagaaatg tagcccaaat aaaaaacgta tttctgatgc tgaaacgtga
     7861 aattgaaaac cgttttatga taatccaatg caaaagaagc cgaaacaaca atcagcaatc
     7921 caataacatc caaataagtg ctattagtaa ttgatttaat agtttttatt gatttttctg
     7981 aaagggaaag taggctcatt caattttgtt tgggtaaata taaagattat tatttgcaat
     8041 cgtaatgcgg aatattacca actaaaatca atgcgcttct aaccaatttt ttcctgctcc
     8101 aatttcaaca tccaaaggaa catccattat aaaagcgttt tccatttcgt gtttaatcat
     8161 tggttggatt ttctccaatt cggagttgtg tacgtcaaag acaagctcat cgtgtacttg
     8221 caacagcatt ttcgatttcc agtttcccga agttagtttt ttatgaatat taatcatcgc
     8281 aattttgata atatcagcgg cagaaccttg aattggggca tttacagcat ttcgttccgc
     8341 accacttttc accatcatat tagcagaatt gatgtctttc aaataacgtc ttcttcctaa
     8401 aaccgtttgc acatagccgt gttttctggc aaaatctact tgattcgaca tataagattt
     8461 cagtttggga taagtggcat aataagcatc aattaactcg gcactttctt tacgtgaaag
     8521 tgaagtttga ttcgataatc caaaagccga aaccccataa ataattccga aattcacggt
     8581 tttggcattg ctacgttgtt ctttggtgac ttcttccaaa ggcacattga atactttggc
     8641 agcggtcgat ttgtgaatat cttcgttatt ttggaatgct ttaatcatat tttcttctcc
     8701 agacaaagca gcaattatac gcaattctat ttgggaataa tcagcagaaa ctaaagtgta
     8761 attttcgtca cgagcaataa aagcttttcg aattaatcga cctctttcag tacgaatcgg
     8821 aatgttttgc aagttcgggt tattcgaact caaacgtcct gttgcggcaa cagtctgcat
     8881 ataatcggtg tggacgcgac cagtcttttt atcgacttgg tttggcaacg catcaatata
     8941 agtgctttgc aattttacca tttgacgcca ttcgagaatg tcgcgcacaa tttcgttgtc
     9001 attggctaaa taagataaaa tttcttcacc agtggcatat tggccggttt tggttttctt
     9061 ttgtttggct ccgccaattt ttaatttgtc gaataaaata tctccgagtt gtttcggt
//
LOCUS       DAOSNN010000087         9117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7571_length_9117_cov_5.637718, whole genome shotgun sequence.
ACCESSION   DAOSNN010000087 DAOSNN010000000
VERSION     DAOSNN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7571_length_9117_cov_5.637718"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLQ70_05945"
     CDS             complement(<1..624)
                     /locus_tag="PLQ70_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34193.1"
                     /translation="MKNNYLLTNFLVFNIIKKLLKGVLSFANMPSEIINNKREDGYVK
                     TEKKSLFREGSFLAQSSFTIKSLFVVAMILWTIGSYAQNYKPFSIRKNIETRGKMIVA
                     GNTILGKDNLPFNDNTKSNEDISMKYIDIDGDPTTFSSSSADLAIPNQADGSPTTCYR
                     VQYAALYWGAVLKSTDGSRANIAKIKLKLPGATTYNTITGQLIYDAIT"
     gene            complement(750..>1690)
                     /locus_tag="PLQ70_05950"
     CDS             complement(750..>1690)
                     /locus_tag="PLQ70_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34194.1"
                     /translation="ALIAAKLAADTKAKADADAKAKAKDEYTKSMDNIAVSLETAKKK
                     QQQLLTNLNATVSAKEKDLQEMKEENDLSDKGIYKEPKPFKSTAGENAALESLTLQIA
                     EVNKIQTEKIKEIEVIYNQRIQKVSDKNDPLNQYYLKAIETLKAEQSQAIQSNKDLVS
                     SLEKIKIATEIEKKRRIKRASFVNAEDRLSQDKAALERIKKTTPFSTTPIKSTDFDYG
                     EEQPNMQILKEDKSVPKGFYLVVAVHSDVAKRDEFLTKAVAAGQNNINFFYDVTTSKY
                     YIYYDKFDNIEEAKKALETKGSTSYNGKMSISKIEN"
     assembly_gap    1691..1790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1791..3216)
                     /locus_tag="PLQ70_05955"
     CDS             complement(<1791..3216)
                     /locus_tag="PLQ70_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQE34195.1"
                     /translation="MKRILPIILFLFAMQILHSQEDGVVSFALPVRNSLKFNKYILNP
                     TFSFVREQNPYVSIYNKREFTQFEDSPQTYLFSYSGRFKENEAIAFSLFQQNYGVLTT
                     FGGVANYAHNVQLRNDSNLTFGLNLGFYKSGLNDSKIITNYPDPSLDNIPSSTLITIN
                     PGINYGLDFLDFGLSLNNLVLYNLQNSKIVADDPEKAIQLHIMHTGYIESYGFFDQSK
                     FSGILRTEFKKDKTVVSGLAMFSIPIGVWAQAGYNSLHGISGGFGVNLTQNISFEYNY
                     EQGMGNLSNFGASHEIALAYKFKNQKYDYADDNEEGSIILPADERTVAPTVKKLTPEE
                     QARKEEQAIEVKARRAEVAQAKLEADAKAKAKLAADAKAKSDALALAKANAPKPKLAT
                     DAKVVDTKPKPDAAAQAKLNADAKAKADALAKQKLAADNKAKADAKAKADALAKEKLA
                     AEEKAKADALIAAKLAADTKAKADA"
     gene            complement(3218..4693)
                     /locus_tag="PLQ70_05960"
     CDS             complement(3218..4693)
                     /locus_tag="PLQ70_05960"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HQE34196.1"
                     /translation="MKFPILNKYFFCFLILVSTLTTIVYGQTIAPQILPFDKICAGTF
                     NEFDATFNHSGFPAGTTFEVQLSDNTGSFTNPISTTIVSSTDITVSQKKITFAVPTTL
                     IGSDVYKLRVKSSTGFISSNFFVKDAVNVGGTLTYFPAYYKPYEESFFINNKQATASI
                     CTGGSVTLAIDNSTPTIPNSSPVNYSSIKYKWYKDNVVVSGATSSSLIAKSAGVYYVM
                     LDYGTCTDSNFRSNSVTVTQSSGGTSASVVSSLGNPFCSAGGATTLGLGSVTGNTYQW
                     YKDNVKINGATTKTLLANQEGFYSVFVDFGGCSSTFTIDLKELKTVSSINIPDTNVIA
                     DGEIIKVIVTTNAASPTFEWYKNNTLISGITTNTYDVTSTGNYKVKITQQSGCAVVDE
                     VSFLVNSVIDLNVVKIPNLISPNGDGINDTWVIPQEFSVGSNTKVEIRNSNGELVFTS
                     DNYLNNWPESPIEFKQINPVFYYFIKPQDGATKKGSITIVK"
     gene            complement(4785..6074)
                     /locus_tag="PLQ70_05965"
     CDS             complement(4785..6074)
                     /locus_tag="PLQ70_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34197.1"
                     /translation="MKKLIKIIVILLLVVCDMNAQQEKGITGFKSWLNNWTEFKPNKV
                     VYNDVSQILTGYIKVDTKLYKRNVYLLQGNVYLMNNAVLTIEPGTVIMADSESKASLI
                     VTKGATIVADGLETDPIVFTSNKVVRKAGDWGGVIILGDAPINKFGNLAAINFDTDAN
                     LSYYGGNNSASNSGVLRFVRIEYAGAKIKGFNNFNGLLLAGVGNKTIFENIMVSYSAG
                     DSFEILGGEVNLNKLISFKSSNDDYKFNYGTQCTIENSLAIRSSYLTSTNGSRCMNVR
                     SYDKQEEVDFTKKQTLVSARNLTLVNNSENVKVDIESGLIKEGVYVAENASIDFKKSV
                     ISGFNPAILLSKEIAINDLNLKKVKLEQMYFNLCKGNIFVENNSNNEDLENWYGNVGF
                     ANVYSKAENSETFIDFMNDKRPDFRLRIGRITVSNED"
     gene            complement(6155..6601)
                     /locus_tag="PLQ70_05970"
     CDS             complement(6155..6601)
                     /locus_tag="PLQ70_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34198.1"
                     /translation="MKAKILYISIAFLFISTASFAQDKKKAPLSIISRTAIIKKYYDK
                     NELSSLPKGELIELCVERVAVLAKTLPYISLATKPGITLEDFGIPKTSEYRKAFEGQE
                     ENTKEYLDNTAEYQKKILPYGDKDDLIKAILFYENILKSLHEFDDM"
     gene            7058..8206
                     /locus_tag="PLQ70_05975"
     CDS             7058..8206
                     /locus_tag="PLQ70_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HQE34199.1"
                     /translation="MQFSEILGQEHIKNHLTRSADLGRIPHAQLFVGPEGCGALPMAI
                     AYAQYILCNNQNGENSGANESCNLKFQKLSHPDLHFVYPTVSTEDVKTKPKSIDFIAD
                     WREFVAQNPYGGLFDWYSVLGVQNKQGEIRVDDAQEILKSLSLKSYEGGYKIMIVWMA
                     DKMNTAASNKLLKLLEEPPEKTIFILISENEEDIIQTIRSRCQVLHFIGLSESVISET
                     LVSRENIDPKTATKIAHQAQGNFNKALHLLKDDNDEFPFEEWFVVWVRAAFKAKGNAA
                     VIADLIQWSEQIATLGRETQKKFLQFCIDIFRQALLHNYETSSLVYMEPAVEGFTIAK
                     FAPFVNGNNIHEIFQELSDAIYHIERNGNAKIILTDLSIKLTRLIHKK"
     gene            8210..8593
                     /locus_tag="PLQ70_05980"
     CDS             8210..8593
                     /locus_tag="PLQ70_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQE34200.1"
                     /translation="MNNTASILLLVFLALTFIQSAYEKIFYWQENIDWLKEHFSKTRL
                     KNLVNPSLILLVIFELISGILSVVGIIELLVNNGRTFGFYGAIFSSITLLMMLFGQRL
                     AKDYDGARTIVIYFIPAVMAVYWLN"
     gene            8621..>9117
                     /locus_tag="PLQ70_05985"
     CDS             8621..>9117
                     /locus_tag="PLQ70_05985"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQE34201.1"
                     /translation="MTPKHPSDTLTILTDLVLPSETNALNNLFGGELLARMDRAASIS
                     AGRHSRRIAVTVSVNHVAFNKAIPLGSVVIIEAKVSRAFKTSMEIYIDVWIEDRESGE
                     RIKSNEAIYTFVAVDETGKPVEVPHIIPETKLEQHRFDDALRRKQLSLVLAGKLNPHD
                     ATELKA"
BASE COUNT         2798 a         1616 c         1424 g         3179 t
ORIGIN      
        1 tgtaatagca tcataaatta gctgacctgt tattgtatta taggtcgttg cacctggtaa
       61 ctttaattta attttggcaa tattagctct actaccatct gtacttttta gcacggcgcc
      121 ccaatacaaa gcggcatact gaaccctata acaagttgtt gggctaccat cagcttgatt
      181 tggaattgcc aaatcggcac tgctggaact aaaggtagtt ggatcgccat caatatcaat
      241 gtatttcatt gatatgtcct catttgattt tgtgttgtcg ttaaaaggga gattgtcttt
      301 tccgagaata gtattccctg ccacaatcat ttttccccta gtttctatat tttttcttat
      361 ggaaaatggc ttataatttt gggcataaga accaatagtc cataaaatca ttgcaacaac
      421 aaaaagtgat tttattgtaa aactactttg cgctaaaaaa gagccttctc gaaaaagact
      481 cttcttttca gtttttacat atccatcttc cctcttattg ttaataattt cagagggcat
      541 attggcaaaa gaaagaactc ctttcaataa cttctttata atgttaaaaa ccaaaaaatt
      601 ggttagtaag tagttgtttt tcatttgaat aaattgtttg ttgtaaacaa aaatcaattt
      661 taatttttat gctttttaaa attagctagt ggccagtacc taattttaaa ataaattgtg
      721 ctccattttt tcaattataa attgaataat tagttctcta ttttagagat agacatctta
      781 ccgttgtaag aggtgctgcc tttagtttcc aaagcttttt tagcttcttc aatattatca
      841 aatttatcat aataaatata gtatttacta gttgtaacgt cataaaagaa gttaatattg
      901 ttttgtcctg ccgctactgc ttttgttaaa aattcatctc ttttggctac atcgctatgc
      961 acggccacta ctaaataaaa tcctttcggt acacttttat cctctttcaa tatttgcata
     1021 ttaggttgct cttcaccata atcaaaatct gttgatttaa ttggagtggt gctaaaagga
     1081 gttgtttttt taatacgttc caaagccgct ttgtcttgtg aaagtctatc ttctgcattt
     1141 acaaaagaag ctcgttttat tcttcttttt ttctcgatct cggtggcaat tttaatcttt
     1201 tctaaagatg aaaccaaatc tttgtttgat tgtatagctt gtgattgttc tgcttttaat
     1261 gtttcaatag ctttcaaata atattggttc aacgggtcat ttttatccga aactttttgg
     1321 attctttgat tgtatattac ttcaatctct ttaatttttt ctgtttgaat tttgtttacc
     1381 tcagctattt gaagagttaa actttctaat gctgcatttt ctccagcagt gcttttgaat
     1441 ggttttggtt ctttataaat acctttatca ctcaaatcat tttcctcctt catttcctga
     1501 aggtcttttt ctttagctga tacagtcgcg ttcaaattag tcaataattg ttgttgtttc
     1561 tttttcgcag tctctaatga taccgcaata ttatccattg attttgtgta ctcatctttg
     1621 gctttagctt tggcatctgc atcagctttg gctttagtgt ctgctgcaag tttggctgca
     1681 ataagtgcat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctgcatcggc
     1801 tttggcttta gtgtctgctg caagtttggc tgcaataagt gcatcagctt ttgctttttc
     1861 ttcggctgct agtttttctt tagcaagagc atcggctttg gctttggcat cagctttagc
     1921 tttattatcc gcagctaatt tttgtttagc aagagcatcg gctttggcct tagcatctgc
     1981 attcaatttt gcttgggcag ctgcatcagg tttaggttta gtatcgacta ctttagcatc
     2041 tgtagctaat tttggttttg gagcattagc tttggctaat gcaagcgcat ccgatttagc
     2101 cttagcatca gcggctagtt ttgctttagc tttggcatcg gcttctaatt ttgcttgagc
     2161 tacttctgct cttctagctt taacctcaat agcttgttct tcttttctgg cttgttcttc
     2221 tggagttagt ttttttactg ttggcgctac cgttctttca tcagcaggta aaatgattga
     2281 tccttcttca ttgtcatctg cataatcgta tttttggttt ttgaatttat aggctaaagc
     2341 tatttcgtgt gaagcaccaa aattggacaa attgcccatc ccttgttcgt aattgtattc
     2401 gaaagaaata ttttgcgtca agttaacgcc aaaacctcct gaaattccgt gtaaactgtt
     2461 gtaaccagct tgtgcccaaa ctccaattgg aatactaaac atggctaaac ctgacacaac
     2521 ggttttgtct tttttaaatt ctgttctgag tatgcctgaa aacttacttt ggtcaaaaaa
     2581 tccataactt tcaatatatc ctgtatgcat aatgtgaagt tgaatcgctt tttcaggatc
     2641 gtctgctact atttttgaat tttggagatt gtataaaacc aaattattca gagacaaacc
     2701 aaaatcaaga aaatccaatc cgtaattgat tcctggattt atggtaatta aagtgctaga
     2761 aggaatgtta tctaaggatg gatctgggta atttgtgatt atttttgaat cgtttaaacc
     2821 acttttgtaa aatcctaaat tgagtccaaa agtaaggttg ctgtcatttc gaagttgtac
     2881 attatgagca taattggcca caccaccaaa agtggttaac acaccgtaat tttgctgaaa
     2941 taaactaaag gcaatggctt cattttcttt aaatcttcct gaataactaa ataaataggt
     3001 ttgcggagaa tcctcaaatt gagtaaactc tcttttatta taaatactta cataaggatt
     3061 ttgctctcta acgaaactaa aggttggatt gagaatgtat ttattgaact ttaacgaatt
     3121 tcttacgggt agtgcaaacg aaacaactcc atcctcttga gaatggagta tttgcatcgc
     3181 aaataaaaat aagataatgg gtaaaattct tttcatatta cttcacaatc gttattgaac
     3241 cctttttagt tgccccatct tgcggcttta tgaaataata aaaaacagga tttatttgct
     3301 taaattcaat tggactttcg ggccagttgt tcaagtaatt atcggaggta aatacaagtt
     3361 cgccattcga attccgtatc tcaactttgg tattactgcc tactgaaaat tcttgtggga
     3421 taacccaagt atcatttata ccatcgccat tcgggctaat caaatttgga atcttgacaa
     3481 cattaagatc tatgacagag ttcacaagaa aagaaacttc gtcaacaact gcacatcctg
     3541 attgttgggt tatcttaact ttataatttc ctgtgctagt tacatcatag gtatttgtcg
     3601 taatgcctga aattaaagtg ttgtttttat accattcgaa tgttggactt gccgcatttg
     3661 ttgttactat aacttttatg atttctccat ccgcaataac gtttgtatca ggaatgttga
     3721 tagaacttac tgtttttaat tcttttaaat caattgtaaa agtgctggaa catcctccaa
     3781 aatcaacaaa aacggaatag aatccttctt ggtttgctag taaggtttta gtagtagctc
     3841 catttatttt cacgttatcc ttataccatt gataggtatt tcctgtaaca gaacccaaac
     3901 caagtgttgt agcaccgcca gctgaacaaa aaggatttcc aagacttgaa acaactgatg
     3961 ccgaagttcc tcctgaagat tgagttactg ttaccgagtt ggatctaaaa ttagaatcag
     4021 tacaagttcc ataatcaagc ataacatagt aaactccggc tgactttgct attaaagagg
     4081 aagaagtcgc tccagaaaca accacattat ctttgtacca tttatactta atactcgaat
     4141 aatttactgg agaactgttg ggaatagttg gggttgaatt atcgattgcc aaagtaacgc
     4201 taccgccagt acaaatacta gctgttgctt gtttgttatt tataaaaaaa gattcttcgt
     4261 atggtttgta ataagctgga aaataggtta aagttccgcc aacattaaca gcgtctttta
     4321 caaaaaaatt actgcttatg aatcctgtag agctttttac tcttaattta tatacatcag
     4381 atcctattaa agtggttgga accgcaaaag ttattttttt ttgactaacc gttatatctg
     4441 tagatgatac aattgttgtg ctaattggat tggtaaaact acctgtatta tctgaaagct
     4501 gtacctcaaa agtagttcca gccggaaaac ccgaatgatt aaaagtagcg tcaaattcat
     4561 taaaagtccc tgcacaaatt ttatcaaacg gtaagatttg tggtgctatt gtttgtccgt
     4621 aaacgattgt ggttaaagtc gaaaccaaaa tcaagaaaca aaaaaaatat ttatttaaaa
     4681 tagggaattt cattgactaa aatatttgct tagccgaaat taaataaata tctttataaa
     4741 aatcgactta attaattcaa aaagttcatt ttatttcgtt ttatttaatc ttcgtttgat
     4801 acggttattc ttcctatacg caatctaaaa tcgggtcttt tatcattcat aaaatctatg
     4861 aatgtctcac tgttttcggc tttggagtaa acattagcaa aacccacatt tccataccaa
     4921 ttttctaaat cttcattatt agaattgttt tcaacaaaaa tattgccttt gcaaagattg
     4981 aaatacattt gctctaattt tactttttta agatttaaat cattaattgc aatttctttg
     5041 ctcaacaata tagctggatt aaaaccagaa ataacacttt tcttaaagtc aattgaagca
     5101 ttttcggcaa cataaacacc ctctttaata agtcctgatt ctatatccac tttaacattt
     5161 tcactattgt tgaccaatgt taaatttcgt gctgaaacta aagtttgttt tttagtgaaa
     5221 tctacctctt cttgtttgtc ataagaacgg acattcatgc atcttgaacc gttagtacta
     5281 gtcaaataag aagagcgaat tgccaatgaa ttttcgatag tacattgtgt tccataatta
     5341 aatttatagt catcattact cgatttaaaa gagatcaact tgttcaaatt aacttctcct
     5401 cctaatattt caaatgaatc accagcagaa taactgacca ttatgttttc aaagatagtt
     5461 ttgttaccaa ctcccgccaa caacaatcca ttaaaattat tgaatccctt aattttagca
     5521 cctgcatatt ctattctcac aaatcttaaa acacctgaat tgctagctga attattacca
     5581 ccataataag ataaattagc atctgtatca aaatttattg ctgccaaatt tccaaatttg
     5641 ttaatcggcg catcaccaag aataatcact ccaccccaat cacctgcttt tctaactact
     5701 ttattagacg taaaaactat cggatcggtt tctaaaccat cggcaacaat ggtagctcct
     5761 ttggtaacga ttagcgacgc tttagactcg ctgtctgcca ttataactgt tccaggctca
     5821 attgttaaaa cggcattgtt cattagataa acattacctt gtagtaaata cacatttctt
     5881 ttatacagtt tagtatcaac ctttatataa cctgttagaa tttgacttac atcattataa
     5941 accactttgt ttggtttaaa ttcggtccaa ttgtttaacc agcttttaaa tcctgttatt
     6001 cctttttctt gttgtgcatt catatcacaa acaactaaaa gaagtatgac gattatttta
     6061 attaattttt tcatgactta tcaactttat attttcaaca cttactccta tttttttaaa
     6121 ttctaataag tgttttaatt tctttttttt cgttttacat atcatcaaat tcgtgtagcg
     6181 atttcaatat gttttcataa aacagaatgg cttttatcaa atcgtcttta tcaccataag
     6241 gcaatatctt tttttgatat tcggctgtat tatccaaata ctcttttgta ttctcctctt
     6301 gcccttcaaa tgcctttctg tattcagagg tttttggaat tccaaaatct tccaaagtaa
     6361 tacccggttt tgtagccaat gaaatataag gcaatgtttt agccaaaacg gctacacgtt
     6421 ctacgcacaa ttcaatcaac tctccttttg gcaaactgga caactcattt ttatcgtaat
     6481 attttttaat tattgctgtt ctgcttataa tacttaatgg agccttcttt ttatcttgag
     6541 caaaagaagc tgttgaaata aataaaaatg cgatactgat ataaagaatt ttagctttca
     6601 taattaatta gatgttttat tttaaacaaa tttacaaaac aaaatttaat ctgcaatttt
     6661 atttaacact aaaaataaat aaattactac aaatgctgaa aaaaaataca aataatcgaa
     6721 taaatatgct ttttatcgga tattttgcgc cgatctaaaa ataatttaat aagtttaatt
     6781 tgattaaaaa aaataaacta ttaacattat gtttttagtt atcaacaaat gtaattttaa
     6841 agctacaaat ttttgagaaa gaaaaatata tctaataaat atgcgtttta tcgaatattt
     6901 tgactctatg aaacaaaaat tcaagtcgct acaaatcgtt tattatttaa aaaaagatta
     6961 attcatactt tttaggtact agtatattaa atcaacaaca acgaaaactt tgccatctaa
     7021 atttcgacac tagaccaatt tcttctatat ttgccttatg cagttttcag aaattttagg
     7081 gcaggaacac atcaaaaatc atttgacacg aagtgccgat ttaggtagaa ttccacacgc
     7141 ccaattgttt gtcggtcccg aaggatgtgg cgctttgcca atggcgattg cctatgcaca
     7201 atatattttg tgcaacaatc aaaacggaga aaattcgggt gccaacgaaa gttgtaatct
     7261 taaattccaa aaattatcgc atcccgattt gcatttcgtt tatccaacag taagcaccga
     7321 agacgtaaaa acaaaaccca aaagcattga tttcattgcc gattggagag aatttgtagc
     7381 ccaaaatcct tacggaggac ttttcgattg gtattctgtt ttaggagttc agaacaagca
     7441 aggcgaaatt cgggtagatg atgcacagga aatcctgaaa tcactttcgc taaaatccta
     7501 cgaaggcggt tataaaatta tgattgtttg gatggccgat aaaatgaaca cagcagcctc
     7561 caacaaatta ttgaaattat tagaagaacc gccagaaaaa accattttca tcctcatttc
     7621 cgaaaacgaa gaagatatta ttcaaacgat tcgttcgcgt tgtcaagttt tgcattttat
     7681 cggtttgagc gaaagtgtga ttagcgaaac cttagtttct cgtgaaaaca tcgatcccaa
     7741 aacggctaca aaaatcgccc atcaagcaca aggaaatttc aacaaagcct tgcatttatt
     7801 aaaagacgac aacgacgaat ttccgttcga agaatggttc gtggtttggg ttcgtgcggc
     7861 tttcaaagcc aaaggaaatg cagctgtgat tgccgatctg attcaatgga gcgaacaaat
     7921 tgccactttg ggaagagaaa cccaaaagaa attcctgcaa ttttgtatcg atatttttcg
     7981 tcaagctttg ctacacaatt atgaaacatc gagtttggta tatatggaac ccgccgtcga
     8041 aggctttaca attgcaaaat ttgcaccttt tgtaaacgga aacaacattc acgaaatatt
     8101 tcaagaattg tctgatgcca tttatcatat cgagcgcaac ggaaatgcta aaataatcct
     8161 gaccgattta tcaataaaat tgactcgttt aatccacaaa aaataagtta tgaataatac
     8221 tgcctccatt ttgcttctcg tttttttggc tcttactttt atacaatcgg cttacgaaaa
     8281 aatattttat tggcaagaaa acatcgattg gctcaaagaa cacttttcta aaactagact
     8341 caaaaatcta gtcaatccct cgctaattct tctcgttatt tttgaactta tttcaggtat
     8401 tttatctgtt gtaggtatca tcgaactttt agtcaacaac ggtagaactt ttggttttta
     8461 tggagccatt ttttctagta tcaccctact gatgatgctt ttcggtcagc gattagccaa
     8521 agattatgac ggcgccagaa ccatcgttat ttactttatt ccagcagtaa tggcggtcta
     8581 ttggctgaat tagaaattaa tttaatttac cacataaact atgactccaa aacatccttc
     8641 agataccttg acgatactaa cggatttagt tttgccaagc gaaaccaacg ctttgaacaa
     8701 cctttttggt ggcgaattat tggctcgaat ggatcgtgct gccagtattt cagcaggaag
     8761 acattcgcgc agaattgcgg taacggtttc ggtaaatcac gtagctttca acaaggccat
     8821 tccactggga agtgttgtca ttattgaggc caaagtatcg agagctttca aaacttcgat
     8881 ggaaatttat attgatgttt ggattgaaga ccgagaatct ggcgaaagaa tcaaatcgaa
     8941 cgaagcgatt tatactttcg ttgccgtgga cgaaaccgga aaacccgttg aagttccgca
     9001 cattattccc gaaaccaaac tggaacagca ccgttttgat gatgccttga gaagaaaaca
     9061 attgagtttg gttttagcag gaaaattaaa ccctcacgat gccactgaat tgaaggc
//
LOCUS       DAOSNN010000088         9088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7606_length_9088_cov_4.170597, whole genome shotgun sequence.
ACCESSION   DAOSNN010000088 DAOSNN010000000
VERSION     DAOSNN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7606_length_9088_cov_4.170597"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..2513
                     /locus_tag="PLQ70_05990"
     CDS             3..2513
                     /locus_tag="PLQ70_05990"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQE34202.1"
                     /translation="MASIIIFLLSFQIINAQQPQKLNSAQIYNQIQKLNFLGSVLYVA
                     AHPDDENTRLITYLSNEVKARTAYLSLTRGDGGQNLIGSQLRDLLGVIRTQELIEARK
                     MDGGEQIFTRANDFGFSKNPEETIEIWDKEKVLSDIIFAIRKFQPDVIINRFDHRTSG
                     NTHGHHSASAILSFESFEKANNPAVFPEQFSLVQPWQTKRQFFNPSWWFFGSKEKFAA
                     ADKSNYLTIETGVYYPSIGKSNQEIAALSRSCHQSQGFGSTGVRGEETEYLEQINGDV
                     LKDKSSIFEGIDTSWNRLKGGKPIGELLTKIEAEFDFKNPSASIPDLVKAYSMIQALD
                     ENHWKTQKTEEIKNTIAACAGLYLEAVSQNQEATPGSIVKLKLEAINRSFAEMQLESI
                     TSLPEQKVTTENIALEYNSLKNISIDLQLPSDIEYTQPYWLKEAGTDGMYTVSNQKNI
                     GIPDVIREVKVIFNTRICGVEIPFERTVVYKYNDGVKGEMYNYLDIVPEVSTTILDKV
                     SLFKNTKRKYIAVKVKAGKDAVKGDLQLELPINWMVLPKSIPFYLEKKGGEQTFYFEV
                     TPPNTPEDAIAKSVAIIDGKRFDKEQIIIDFDHITKQQVLKPSESKFLRFDLKTKDQK
                     IAYIMGAGDEVPNCLRQMGYKVTLIHPEELSPEKLANFDVVVTGIRAFNTIKVLANRQ
                     NILFEFVKSGKTMISQYNTPDIIYNIAPYSMNLSQDRVTDENAEVRFLEPNHTVLNSP
                     NKITSKDFQGWKQEQGLYYPNQFDKAFTPILSSNDKGETPKNGALLIAPYGKGYYIYT
                     GLSLFRELPEGVSGAYKILANMISLQNPVVIPTEKVKL"
     gene            2635..4356
                     /locus_tag="PLQ70_05995"
     CDS             2635..4356
                     /locus_tag="PLQ70_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQE34203.1"
                     /translation="MQLFDWIVLAVTLLFIVIYGVWKTKGSKNVEDYILGSNETPWHT
                     VGLSVMATQASAITFLSTPGQAYHDGMGFLQFYFGLPIAMVVICVTFIPLYHKYKVFT
                     AYEYLEKRFDLRTRSLAAVLFLVQRGLGTGLTIYAPAIILSALLGWNLTIMNIVIGVL
                     VIIYTFSGGTKAVNVTQKQQMFVIMFGMFITFFLILYFLPNDMTFTSALHIAGANDKM
                     DIVNFSFDPEEKYTIWSGLTGGFFLALAYFGTDQSQVGRYLSGKSVRESQMGLIMNGL
                     LKVPMQFFILLTGVMVFVFFQFNPVPLNFNPNNKIIVEKSKYKSEYNALEKKLEALSE
                     DKKVINLLYIDQLNQDYDNPILRKELIILSSREKDLRDHAKELILKADKRSETNDKDY
                     VFFHFILHYLPKGLIGLLLAVIISAAMSSTASGLNALASTTAIDIYKRNVKEAKTERH
                     YVNVTKFFTLFWGIVAILFASIGTLFENLIQLVNIVGSIFYGTVLGIFLVGFYIKHVQ
                     SKAIFYSAVTSQITIFIIYYFAIYIYPSGEEKLGYLWLNFIGAMLTIVLSMIMQIIFR
                     KEKVTIA"
     gene            4711..6066
                     /locus_tag="PLQ70_06000"
     CDS             4711..6066
                     /locus_tag="PLQ70_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQE34204.1"
                     /translation="MKKNVLHFCFFMFSTFAFSQAGHIMQGVGAFNMSMGGASTGQPL
                     DISGALQWNPASISAFNDKIIKLDAGLFFSSPELSSSLPAGMMGTGSPAVSGVTKDAR
                     DPSIMPALAMVWGKEGSKHTFGFSGFGISGFGVTFPEELNQPLSASFNPSINSNPINY
                     PQKAGGFGRIKSDYMLLQIGLTWAYEITDKLSVGVEPTFDYGTLQLMPNPTASPSAAG
                     YPYTNKASTAGFGAQFGLYFDSKKGFKAGVSYKTTQNLSELEFKNTYLDNSKGTNKFD
                     MDYPAILSFGLGYSKSDFDLALDYRMVDYKNTNGFSETGWTPTASVKGFGWDNVSILS
                     AGIQYKGIDKLPLRLGYTYSSNPISSDVAFFNIPATAIIKNAFQFGFSYAASSNITLD
                     AVYHHGSSDGKTSGQMLNPMMASPSNPYGAVPGTTIGYEMTTDLVMVGISYKFNAKQT
                     K"
     gene            6302..8539
                     /locus_tag="PLQ70_06005"
     CDS             6302..8539
                     /locus_tag="PLQ70_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQE34205.1"
                     /translation="MNSSRRDFIKISMLGAGAVAVAGSLYSVDVFGGNKEDFNYDRPD
                     LKRYPTYCDVCFWKCAGWSYVDEKGTIKKIIGNDIDTHSKGRLCTRGTGGLGMHFDND
                     RLKTPLIRVSKKGEKSVFREASWEEAISLVASKMKDVKAKYGAESFALLKHGAIGSHL
                     EHLFSSYGSDTVAEPAFAQCRGPRDEGLKLTFGETCGSPESTDMRDSKCLVFIGSHIG
                     ENMHNTQVQEVSEAIDKGVTIITVDPRLSTVASKSKYWLPIRPATDMALLLAWMNVII
                     FEDLYDKEYVAKYTTGFDELKEHVRQFTPEWAYSITDLTPKMIRDSAREMGKAAPATL
                     IHPGRHSTWYGDDTQRTRAIGILNALLGTWGRRGGFYFNEKAVVPKFPHPEYPEPAWK
                     AKDLNVNLPFANQGVTNEVIRASIPGENTPHQVKAWMVAGTNLTQSVPTEKNLMMKAI
                     EALEFLVVIDIYPVEITGYADVVLPECSYLERYDDLRNSDSRTPSIALRMPAAAPRWE
                     SKPGWWIGKQIGLKLGLADYYKFNDYTDILDWQFKQMGTSLEEMKHIGVKNLPRSGNP
                     LYIEDGADHIFKTDSGKIELYSQKLKDVGLDPMPVYTAHEQPPKDYFRLNYGRAPMHT
                     FSKTVNSPNLNDLRSENALWVNPKVGKIWNLKSGQPVWLKNQDDVVSSFPIKVRLTER
                     IRWDSVYMVHGFGQKNPEMKRSYGKGVNDTEMITKLMVDPLMGGTGMRGNFVTILTEN
                     PKETV"
     gene            8541..9077
                     /locus_tag="PLQ70_06010"
     CDS             8541..9077
                     /locus_tag="PLQ70_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQE34206.1"
                     /translation="MRYVMVIDTLKCVGCSDCVVACQTENDVPTGYCRDWVTESVTGT
                     YPEVDIELRSERCNHCDNAPCVRTCPTEASHIIEGGIVLVDRDLCIGCGACIESCPYD
                     ARYRHPDLGIVDKCTFCIHRVKKGQDPACVSVCPTNCMYFGDLDDSNSKVAQLLAVRK
                     YKVLAKEAGTSPNVYYLI"
BASE COUNT         2952 a         1470 c         1774 g         2892 t
ORIGIN      
        1 tgattgcatc cattattatt tttcttcttt ctttccaaat aataaatgcg caacaacctc
       61 aaaaactcaa ttcggcacaa atttataatc aaattcagaa acttaatttt ctgggttcgg
      121 ttctgtatgt tgccgcacat cctgatgacg aaaacacccg attaattact tatttgtcta
      181 atgaagttaa agcaagaacc gcctatttat ctttgacaag aggcgatggt ggacaaaacc
      241 tgattggttc tcagcttaga gatttattgg gcgtaattcg aacacaagaa ttaattgaag
      301 ccagaaaaat ggatggtggt gaacaaattt ttactcgtgc caatgatttt ggtttttcca
      361 aaaatccaga ggaaactatt gaaatttggg ataaagaaaa agtactttct gatattattt
      421 ttgccattcg aaaatttcaa cccgatgtaa ttatcaatcg ttttgatcat agaacgtctg
      481 gaaatactca tggtcatcat tcggcatcgg caattttgag ttttgaaagt tttgaaaaag
      541 ccaataatcc agctgttttt cctgagcaat ttagcttggt acaaccttgg caaaccaaac
      601 gtcaattttt taatccatcg tggtggtttt ttggaagcaa agagaaattt gctgcagccg
      661 ataaatcaaa ttatcttact attgaaactg gtgtttatta tccatctatt ggaaaatcaa
      721 atcaggaaat tgctgctttg agtcgcagtt gtcatcaatc acaaggtttt ggaagtacgg
      781 gagttcgagg cgaagaaaca gaatatttag aacaaataaa tggtgatgtt ttaaaagata
      841 aatcgtctat ttttgaagga attgacacgt cttggaatcg tctaaaagga gggaagccta
      901 ttggcgaatt gctaactaaa attgaagccg aatttgattt taaaaaccct tcggcgagta
      961 ttccagattt agtaaaagcc tattcgatga ttcaagcatt agatgaaaat cattggaaaa
     1021 ctcaaaaaac ggaagagatt aagaatacaa ttgcagcttg tgcaggtttg tatcttgaag
     1081 ctgtgtcgca aaatcaggaa gcgactccag gaagtatagt aaaattgaaa ctggaagcca
     1141 tcaatagaag ttttgcagag atgcaattag aatcgattac tagcttgcca gagcaaaaag
     1201 taaccactga aaatatcgcg ttagaataca atagtttgaa gaatatttcg attgatttgc
     1261 aattgccttc tgatatagaa tatacgcaac cctattggtt aaaagaagct ggaaccgatg
     1321 gaatgtacac tgtttcaaac caaaagaata ttggaattcc agatgttatt cgagaggtaa
     1381 aagtaatttt taacactcga atttgtggag tcgaaatccc ttttgagcga actgtggttt
     1441 acaaatacaa tgacggagta aaaggcgaaa tgtataatta tttagatatt gttccagaag
     1501 tttctactac tattttagat aaagtatcac tttttaaaaa cactaaaaga aaatatattg
     1561 ctgtaaaagt taaggctgga aaagatgctg taaaaggtga tttacaactc gaattaccaa
     1621 taaactggat ggttttgcca aaatcaattc cattttattt agaaaaaaaa ggtggggaac
     1681 aaacattcta ttttgaagta acaccaccta acactcccga agatgctata gccaaaagtg
     1741 ttgctataat tgatggtaaa cgttttgaca aagaacaaat tatcatcgat tttgatcaca
     1801 ttaccaaaca acaggttttg aaaccttcgg aatctaagtt tttgcgtttt gatttaaaga
     1861 caaaagatca aaaaatagct tatattatgg gggctggaga tgaagttcct aattgtttga
     1921 ggcaaatggg gtataaagtg actttaatcc atcctgaaga actatctccc gaaaaattag
     1981 ctaattttga tgtagttgta actggaattc gagcttttaa taccattaaa gtattggcta
     2041 atcgacaaaa tattcttttt gaatttgtga aatcaggcaa aacaatgatt tcacaataca
     2101 acacgcccga tattatttat aatattgcgc cttattcaat gaatctttct caagacaggg
     2161 ttacagatga aaatgctgaa gttcgatttt tagaaccaaa tcatacagta ttaaattcgc
     2221 caaataaaat tacttccaaa gattttcaag gttggaaaca agaacaagga ttgtattatc
     2281 ccaatcaatt tgataaagct tttaccccaa ttttatcttc aaatgataaa ggtgaaacgc
     2341 ccaaaaatgg tgctctatta attgcacctt acggaaaagg atattatatt tacacgggtt
     2401 taagtctttt tagagaatta cccgaaggag tttcaggggc ttataaaata ttggccaata
     2461 tgatttcttt gcaaaatcca gtagtaattc ctactgaaaa agtgaaactt taaacacgca
     2521 atgatggaaa ataaaaaagt aaatacttgg aaaaaaagct acacttgggt tttggtggct
     2581 aatgctattt atattttatt tttttatttt ttgatgaaat tatattccta gcctatgcaa
     2641 ctatttgact ggattgtact tgctgtaacc ttacttttta tagtgattta tggcgtttgg
     2701 aaaacaaaag gaagtaaaaa tgtagaagat tatattcttg gaagtaatga gactccttgg
     2761 catacggtag gtctttcggt tatggccaca caagccagcg cgataacttt tctttcaaca
     2821 cctggtcagg cttatcatga cggaatgggg tttttgcaat tttatttcgg tttacctatt
     2881 gcaatggtgg ttatttgcgt gacttttatt cctttatatc acaaatacaa ggtttttacg
     2941 gcatacgaat acctcgagaa gcgcttcgat ttgagaacac gttcgcttgc agcagttctt
     3001 tttttggttc aaagaggatt aggaaccgga ttgaccattt atgcgccagc tattatttta
     3061 tcggcattat taggttggaa tttgacaata atgaatattg taataggagt tttagtaatt
     3121 atatacacct tttcaggggg aacaaaagcg gtaaatgtca ctcaaaaaca gcagatgttt
     3181 gttattatgt ttggtatgtt tatcactttt tttctgattt tgtatttttt acccaacgat
     3241 atgacattta caagcgcttt gcatattgct ggggcaaacg acaaaatgga tattgttaat
     3301 ttttcatttg acccagaaga gaaatatact atttggagcg gactcaccgg aggttttttc
     3361 ttggctttgg cttattttgg aaccgaccaa tcgcaagtag gacgctattt gtcaggaaaa
     3421 tcagttcgag aaagtcaaat gggtttgatt atgaatggct tgttaaaagt gcctatgcaa
     3481 ttttttattt tgctaacggg agtgatggtt tttgtatttt ttcaattcaa tccggttccg
     3541 ttaaatttta atcccaacaa taaaataatt gtagaaaaat caaaatacaa atcagagtat
     3601 aatgcattag aaaaaaaatt agaagcactt tcagaagata agaaagtaat taatttgttg
     3661 tatatcgatc aactcaatca agattatgac aaccccattc ttcgaaaaga attgataata
     3721 ttatccagta gagaaaaaga tttgagagat cacgccaaag aattaattct aaaagccgat
     3781 aaacgaagtg aaaccaatga taaagattat gtattctttc attttatttt gcattatttg
     3841 cccaaaggat taataggttt gttgctggca gtaattattt cagcggccat gtcttctacg
     3901 gcttcgggtt taaatgcatt ggcttcgact acagcaattg atatctataa aagaaatgtt
     3961 aaagaagcaa aaactgaaag acattatgta aatgttacta aatttttcac tttattttgg
     4021 ggtattgttg ccattctttt tgcaagtatt ggaactttgt ttgaaaacct gattcaattg
     4081 gtcaatattg tgggatccat attttacgga acggttttgg ggatatttct tgttgggttt
     4141 tacatcaaac atgttcaatc caaagctatt ttttacagtg ctgtaactag ccaaattacc
     4201 atatttatta tctactattt tgccatttat atttatccaa gtggtgaaga aaaattaggt
     4261 tatttgtggc tcaactttat tggagcaatg ttgactattg tattgtctat gattatgcag
     4321 ataattttta gaaaagaaaa agtgactatt gcttaaatat tttttttttg gaatttgaat
     4381 ttcataaaac tgtctttttg aggcagtttt ttttgtttta aaaactaact ttctttaaat
     4441 catttgatta ataaaatcat ttttctcaat tttaagtatg aatagaaaag aagacaaaat
     4501 tatcctcttt ttgtaaacta tgtctcgtta acaatttatt aaaaaaatag ttgattatct
     4561 tcaaaaacag cgagttatgt aacatttgtt acatattggt tttccttatt ataataattt
     4621 tatcctatta aatcaatggg tcttgttcta aataaaaaat aggattcggt tgttttttat
     4681 ttatcctatt ttaatcaata ctttttaatc atgaaaaaaa acgtactaca cttttgtttt
     4741 tttatgtttt cgacttttgc tttttcacaa gcaggtcata ttatgcaagg agttggagct
     4801 ttcaatatgt ctatgggagg cgcatctacg gggcaacctc ttgacatttc gggcgcattg
     4861 caatggaatc cagcttctat ttcggcattc aatgacaaaa tcatcaagtt agacgcaggt
     4921 ttgttttttt cttcaccaga attgagttct agtttaccag caggaatgat gggtacgggt
     4981 tctcctgccg tttctggagt tactaaagat gccagagacc cttctataat gcctgcttta
     5041 gcaatggttt ggggcaaaga aggaagcaaa cacacatttg ggttttcagg tttcggcata
     5101 agtggctttg gagttacttt tcctgaagaa ttaaaccaac ctttgagtgc tagttttaat
     5161 ccttcaataa attcaaatcc tattaattat ccacaaaaag caggtggttt tgggagaatt
     5221 aaatccgatt atatgctgtt gcaaattggt ttgacttggg cttatgaaat caccgataaa
     5281 ctttctgtgg gggttgagcc aactttcgat tatggaactt tgcagttaat gccaaatcca
     5341 acagcaagtc catcagcagc gggatatcct tatacaaata aagcttcaac tgctggcttt
     5401 ggggctcaat ttggtttgta ttttgactcc aaaaaaggat tcaaagcggg agtttcatac
     5461 aaaactactc agaatttaag cgaattagaa ttcaaaaata cttatttaga taattcaaaa
     5521 ggaactaata aattcgatat ggattatcct gcaatacttt cttttggttt aggatattca
     5581 aaaagcgatt ttgatttagc tttagattat agaatggtcg attataaaaa taccaatggt
     5641 ttctctgaaa caggttggac accaacagct tctgtaaaag gatttggctg ggataatgtt
     5701 tctattttgt ctgccggaat tcaatataaa ggaatcgata aactcccctt aagactggga
     5761 tatacctata gttcaaatcc tataagtagc gatgtagcct tctttaatat tcctgcaaca
     5821 gctatcatta aaaatgcttt ccaatttgga tttagttatg cagctagttc taatatcact
     5881 ttagacgctg tttatcacca cggttctagc gacggaaaaa cttcgggtca aatgcttaat
     5941 ccaatgatgg cttcaccttc aaatccttat ggagcggttc caggaacaac cataggttat
     6001 gaaatgacta cagatttagt gatggttgga atctcgtata agtttaatgc aaaacaaaca
     6061 aaatagtcag gttttgattt taatatatta aaagcggttt ctgcaaaagg aaatcgcttt
     6121 ttttagataa taatcgatag aaaaatagtt gtccaactaa gttcgaattt gcgaaattta
     6181 tttttataga tgccttgttg tgtaacattt gttactccta aaagtagtga aataaagtaa
     6241 atttgaggtg tctattcata aaggcaatag actattacaa ttaaaaatca aatatttaaa
     6301 tatgaattca tcaagaaggg attttataaa aatttcaatg ctaggagcag gagcagttgc
     6361 cgttgcaggt tcgctttatt cagttgatgt ttttggtgga aataaagaag atttcaatta
     6421 tgaccgcccc gatttaaaac gatatcctac ctattgcgat gtttgctttt ggaaatgtgc
     6481 cggctggtct tatgtggacg aaaagggaac aatcaaaaaa attattggca acgacatcga
     6541 tacccacagt aaagggagat tatgtactcg tggaactgga ggtttgggaa tgcatttcga
     6601 taatgaccga ctcaaaacgc cactaattag agtatcaaaa aaaggagaaa aatcagtatt
     6661 tcgcgaagcc tcttgggaag aagcaatttc cttagtagca agcaaaatga aagatgtcaa
     6721 agctaaatac ggtgcagaaa gtttcgcact tctaaaacac ggagcgattg gcagtcattt
     6781 ggagcatctt tttagctctt acggatcgga tacagttgcc gaacccgcct ttgcacagtg
     6841 tcgtggtcct cgtgacgaag gattgaaact cacttttggt gagacttgtg gctcgcccga
     6901 atctaccgat atgcgagatt ctaaatgttt ggtatttata ggttcacata ttggcgaaaa
     6961 tatgcacaat acgcaagtgc aagaagtttc cgaagccatc gacaaaggtg ttactattat
     7021 caccgttgac cctcgtttat caactgtggc aagcaaatcg aaatattggt tgcctattcg
     7081 ccctgcgacc gatatggctt tgctattggc ttggatgaat gtgattattt ttgaagattt
     7141 gtatgataaa gagtatgtag ctaaatacac cactggattt gacgagctca aagaacacgt
     7201 tcgacaattt acgccagaat gggcgtacag catcactgat ttgactccca aaatgattcg
     7261 ggattcggct cgtgaaatgg gaaaagcagc tcccgcaacc ttgattcatc ccggacgaca
     7321 ttctacttgg tatggcgatg acacccaacg tacccgtgct attggaattc taaatgcact
     7381 tttaggcact tggggacgcc gtggtgggtt ttattttaat gaaaaggcag ttgtgccaaa
     7441 atttccacat cctgaatatc cggaacctgc ttggaaagcc aaagatttga atgtcaatct
     7501 gccgttcgcc aatcaaggag tgaccaatga agtgattcgg gcttcgattc ctggcgaaaa
     7561 tacaccacat caagtgaaag cttggatggt ggcaggaaca aatttgacgc aatctgttcc
     7621 caccgagaaa aacttaatga tgaaagctat tgaagccttg gaatttttgg tagttattga
     7681 tatttatcct gtagaaatta cgggttatgc cgatgtggtt ttacccgaat gttcctattt
     7741 agaacgatac gacgatcttc gaaattcgga ttcacgaacg ccttctatcg ctctgagaat
     7801 gccagctgcg gctcctcgtt gggaatcaaa accgggttgg tggattggca aacaaatagg
     7861 tttaaaatta ggtttagcgg actattataa attcaatgat tataccgata ttttggattg
     7921 gcaattcaaa caaatgggaa cttcattgga agaaatgaaa cacattggag taaaaaatct
     7981 tccaagatct ggaaatccat tgtatattga agatggagca gaccatattt ttaaaaccga
     8041 ttccggtaaa attgaattgt attcccaaaa actaaaagat gttggacttg atccaatgcc
     8101 tgtttacacc gctcacgaac aaccacccaa agattatttc cgtctgaatt atggtagagc
     8161 tccgatgcac acctttagca aaacggtcaa cagcccaaat ctgaatgatt tgagaagtga
     8221 aaatgcatta tgggtcaatc caaaagtggg taaaatttgg aacctgaaat caggacagcc
     8281 cgtttggtta aaaaatcaag atgatgtagt ttcttcattt cctataaaag tgcgtcttac
     8341 ggagcgaatt cgttgggatt cggtttatat ggtacacgga tttggacaaa aaaatccaga
     8401 aatgaaacgt tcctacggaa aaggcgtcaa cgataccgaa atgattacca aattgatggt
     8461 cgatccattg atgggaggta ccggaatgcg aggaaatttt gtaacaattt taacggaaaa
     8521 ccctaaagaa acggtctaag atgagatatg taatggtcat agatacgcta aaatgcgtag
     8581 gatgtagcga ctgtgtagtg gcttgtcaaa cagaaaatga tgttccaacg ggatattgca
     8641 gggattgggt taccgaatcg gttacaggaa cctatcccga agtggatata gaattaaggt
     8701 cagaacgctg caatcattgc gacaacgcgc cttgcgtaag aacttgccca accgaagcca
     8761 gccacattat cgagggcgga attgttttgg tggacagaga tttgtgtatt ggatgcggcg
     8821 cttgtatcga atcttgtcct tacgatgccc gatacagaca ccccgatttg ggaatcgttg
     8881 ataaatgtac tttttgcatt caccgggtga aaaaaggaca agatccagcc tgtgtttcgg
     8941 tttgtcctac gaactgtatg tattttggcg atttggatga ttccaatagc aaagtcgctc
     9001 aactattagc agtacgaaaa tataaagtct tggcaaaaga agctggaaca tcacccaatg
     9061 tttattattt aatttaaaac caaagatt
//
LOCUS       DAOSNN010000089         9058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7641_length_9058_cov_6.778296, whole genome shotgun sequence.
ACCESSION   DAOSNN010000089 DAOSNN010000000
VERSION     DAOSNN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7641_length_9058_cov_6.778296"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLQ70_06015"
     CDS             complement(<1..290)
                     /locus_tag="PLQ70_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HQE34207.1"
                     /translation="MAYLFTSESVSEGHPDKVADQISDALIDNFLAFDPESKVACETL
                     VTTGQVILAGEVKSETYLDVQTIARDVIKKIGYTKSEYMFEANSCGVLSAIH"
     gene            984..2264
                     /locus_tag="PLQ70_06020"
     CDS             984..2264
                     /locus_tag="PLQ70_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HQE34208.1"
                     /translation="MSTPKFATNALHAGHDVTKTAGTRAVPIYQTSSYVFNNSDHAAN
                     LFGLAEAGFIYTRLNNPTNDVLEQRLAALEGGIAAVVTASGTAAIATSLLVLLKTGDH
                     IVASNSLYGGTYNLFSNTLPRLGITTTFVDPSDASNFTKAANENTKVFFAESLGNPKL
                     DVLDLKAIAAEAKAFKVPFIVDNTVPSPYLLNPIEHGANIVIHSLTKYIAGNGTSLGG
                     AVIDAGTFDWSSGKFPEFTEPSDSYHGLVYHEALGNAAFIAKVRIEGLRDFGAALSPF
                     NAFQILQGLETLEVRVQKHSQNGLELAQWLEKQEAVAWVNYPGLKSSKYYDLATQYLP
                     KGQNGLLTFGLKKGFVAAKKVADETKLFSLLANIGDTKSLIIHPASTTHQQLSDAEQV
                     TTGVTKDLIRLSVGLEDIEDLKADLKAVFDNLIA"
     gene            2342..2551
                     /locus_tag="PLQ70_06025"
     CDS             2342..2551
                     /locus_tag="PLQ70_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34209.1"
                     /translation="MKTLKYIARVFPFFKSLSKKTEKDTEETTNFEFAYPKFGINLYQ
                     SSAVKKESKSLLTQMYSEENEVLFI"
     regulatory      2717..2823
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2900..6286
                     /gene="thrA"
                     /locus_tag="PLQ70_06030"
     CDS             2900..6286
                     /gene="thrA"
                     /locus_tag="PLQ70_06030"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HQE34210.1"
                     /translation="MENKPTTIKIQNFTTESGVLIPSINLSYQVFGQVLNTAPIVLVN
                     HALTGNSQVVGENGWWNNLIGDNKTIDTNKYSILAFNVPGNGFDKTYIENYLDFTARD
                     VARLFLEGIRFLKVKELFALIGGSVGGGIAWEMIALDPKLTHNFFPIASDWKSTDWLI
                     ANCYLQEKILCNSSKPIEDARIHAMLCYRTPESFKEKFQRNSTDSFESFQVESWLNHH
                     GEKLQKRFQLSAYKMMNQLLKTIDITRGKTSFEDLISQVDANIYIIGINSDLFFTANE
                     NKETYNEIKKFKHNVFYSEIESQHGHDAFLMEYEQLNNLLDVVFKNKKNKMKILKFGG
                     KSLANGEGINKVFDIIIDKKSKGENIAVVVSARGNATDELEDILTIAAKNGNYKPLLE
                     SFKSYQQDEYKVDFSEEFAILDKLFEGVSLIGDYSEKIKDQVLSQGEVISAKLITAVL
                     NEKGIAARFADTRELIKTDSNFGDAQPIEQISKKNVIHYFKENAAVVNIVTGFIGSNS
                     SGETTTLGRNGSNYSASLIANYVDAEELQNYTHVDGIYTANPDLVPDAKKIDRLSFNE
                     ANEIANFGATILHAKTIIPLLEKNIPLRILNTFNHENQGTLITSKSNKEGIKTLSVLE
                     NVALVNLEGRGLLGKTGVDARIFKVMGDNDISVSIISQGSSERGIGLVVNSNQATKAM
                     IELEKEFENDFYSKDVNKISITDDVSVISIIGQDLSTFHKPYTALIKNRIVPILFNNT
                     VTGKNVSLVVKKAQLNKALNVIHGEIFGVSKKINIAVFGHGLVGGTLINQILESAPAI
                     EKRKDIKLNVFAIANSKNLLLNKNGVSPNWKNEIQTNGFSYTIDDVIAFANANNLENL
                     IAIDNTASAAFVEHYTTLAENSFDLISSNKVANTLSYKFYKDLRKVLAENQKNYLYET
                     NVGAGLPLIDTIKLLHLSGENITKIKGVFSGTLSYLFNNFSAKDVPFSEILKEAIDNG
                     YTEPDPREDLCGNDVGRKLLILARELDLQNEFEEISIQNLIPEHLREGNVADFLNKLK
                     EFDPIYNKIKEEQEPNHVLRYIGELSGDLQNDKGNLEVKLVSVPSDTALGGLKGSDSF
                     FEIYTESYGDRPIVIQGAGAGSAVTARGVFGDLLRLSEKG"
     gene            6372..6806
                     /locus_tag="PLQ70_06035"
     CDS             6372..6806
                     /locus_tag="PLQ70_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQE34211.1"
                     /translation="MKVTLNRVNENFHFELKNERGHIVNVDARPDFGGNDMGPSPMEL
                     VLMGVAGCSGIDMISILKKQRQEITSFKADVEGERVQVGDAKPFKDIYVVFYLEGNIK
                     EDKALKAAQLSFEKYCSVAKTVEPTATIHYKVVLNGSPLEKI"
     gene            6897..8069
                     /locus_tag="PLQ70_06040"
     CDS             6897..8069
                     /locus_tag="PLQ70_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQE34212.1"
                     /translation="MNEQEFGFETQAIRNQLERTQYLEHSVPLYLTSSFVFEDAEDMR
                     ASFADEKDRNIYSRYSNPNTNEFVDKVCQMEGAASGFAFASGMAAVYSTLAALLNSGD
                     HIVSAGSVFGATHSLFVNYFPKWGIETSYFDIDKPETIESFIKPNTKILFAESPTNPA
                     VDIIDLELLGAIAKKHNLILIIDNCFATPYLQQPIKWGAHLVVHSATKLMDGQGRVLG
                     GITVGDAELIQKIYLFSRLTGPSLSPFNAWVLSKSLETLAIRLDRHCENALKVAEFLE
                     QHPNVSLVKYPFLKSHPQYEIAKKQMKLGGNIVAFEIKGGIEAGRAFLNKIKLCSLSP
                     NLGDTRTIVTHPASTTHSKLSVEERLAVSITDGLVRVSVGLETVADIIADLEQALG"
     gene            8321..8731
                     /locus_tag="PLQ70_06045"
     CDS             8321..8731
                     /locus_tag="PLQ70_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34213.1"
                     /translation="MTEIFPSLIESTCIDSRVLANLPPVGKSIYDKNGNNIGLDTTNI
                     KREREIYEREELRIKNDTSKIIFAFDSKIKRNRNNVEEDFEKHFSGAKIFESKKEQNL
                     EYDFDFKELKIKNKFVLENISEFPKGKGEIWKIL"
     assembly_gap    8756..8765
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8766..8964
                     /locus_tag="PLQ70_06050"
     CDS             <8766..8964
                     /locus_tag="PLQ70_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34214.1"
                     /translation="WKAKYNFIFSGVVFFTRIQFDKDKKFGILDGGFACGRLCGQGSR
                     IYIKKINNKWVIDKIEGTWVS"
BASE COUNT         3196 a         1408 c         1723 g         2721 t
ORIGIN      
        1 tcgtgaatgg cagaaagaac accacaagaa ttggcttcaa acatatattc acttttggta
       61 taaccaattt ttttgataac gtctctagca atagtttgaa cgtctaaata agtttcagat
      121 ttcacttcac cggccaaaat aacttgtcca gtagtcacca aagtttcaca agctaccttt
      181 gattctgggt caaaagccaa gaaattatca attaacgcat ccgaaatttg atccgcaact
      241 ttgtctggat gtccttcact cacagattct gacgtaaata aataagccat aataatataa
      301 ttttaaaatt aagcgaggaa gaaaaaaaca attgctggaa aatagtgcta aaggagagtt
      361 gctgctttag cattttttta acgaggttgc aatcagttca aatttttcct ctaatcggct
      421 gcaaaggtat gaaaccaaat tgaattgcaa attaatctta actttttttt gatgttaaaa
      481 attaaggcat agaatccttt gatttgtaga ttgtttaaag atttgcctgt ttgtgtaatt
      541 ttttttaaaa aatatttgga tttataaata atagtttaga tatttgtcac aacaaaaaag
      601 gaaagcaaaa tgattttaaa tgtatgtaaa atgtcgaaac agattaagga tatatcctgt
      661 gggtcgccta ttgcataaat ttgatttcaa attcacatag caaaaagctt cccaactaac
      721 gggaagcttt tttttttgca acacaaataa ttaaaataat ttacaatgaa caacttacaa
      781 caaaaacaaa tgaacaaatg caatgtcaaa tcgatgattt gtgcaaagtc tatgatgtgt
      841 gctcgtgtct gttgattccg aaagtataag ttgaacaaaa cttaaaaccc ttttggatat
      901 gtgtctaaaa gggttttttg ttttaaagca ttgaacaaac attaaaaaaa taaaaacaat
      961 taaaaaaata aaataaagaa aatatgagca caccaaaatt cgctacaaac gcattacacg
     1021 caggacacga cgttactaaa acagcaggaa caagagcggt acctatttat caaaccagtt
     1081 cttatgtgtt taataactcg gatcacgcgg ccaatttatt tggacttgca gaagcgggat
     1141 ttatatatac cagattaaat aatccaacaa atgatgtttt ggaacaacgt ttagcagctc
     1201 tggaaggcgg aattgcagca gtagttaccg catcaggaac ggcggctatt gccacttcgt
     1261 tattagtctt gttgaaaact ggcgaccaca ttgtggcttc caatagttta tacggcggaa
     1321 cttataattt gtttagcaat actttgccaa gattaggaat taccaccacg tttgtagatc
     1381 catcagatgc aagcaatttt accaaagctg ctaatgaaaa taccaaagta ttttttgcag
     1441 aaagtttagg gaatccaaaa ttagatgttc tggatttgaa agccatagca gctgaagcca
     1501 aagcattcaa agtgcctttt attgtggaca acacagttcc ttcgccttat ttgctaaatc
     1561 cgattgaaca cggagccaat attgtgattc actccttgac caaatacatt gcaggaaacg
     1621 gaacttcgct tggtggcgcg gttattgatg caggaacatt cgattggagt agcggaaagt
     1681 ttcctgaatt tacagagccc tcggacagtt atcacggatt ggtttatcac gaagcattag
     1741 ggaatgccgc ttttattgcc aaagttcgga ttgaagggtt acgagatttt ggagctgctt
     1801 taagtccgtt taatgctttt cagattcttc aaggtttaga aactttagaa gtccgtgtcc
     1861 agaaacacag ccaaaatgga ttggaattag cgcagtggtt agaaaagcag gaagccgtgg
     1921 cttgggtcaa ttatccagga ttgaagtcca gtaaatatta cgatttggca acacaatatt
     1981 tacccaaagg acaaaacgga ttgctaactt tcggccttaa aaaaggattt gtagccgcca
     2041 aaaaagtagc cgacgaaacc aagttatttt cgcttttagc caatattgga gacaccaaat
     2101 cattgattat ccatccggcg agtaccacgc atcaacagtt atccgatgca gagcaggtta
     2161 ccacgggagt taccaaagat ttaatcagac tctcggttgg attggaagac atagaagatt
     2221 tgaaggccga tttaaaagcc gttttcgaca atttaatagc ataaatcaaa catatttgaa
     2281 cataggaaaa actgaattcg atatgtttta aaaaaaacaa aaacattaaa aatatacgat
     2341 tatgaaaaca ctaaaatata tagcaagagt atttcctttt ttcaaaagtt tgagtaaaaa
     2401 aacagaaaag gatacagagg aaacgactaa ctttgaattt gcatacccaa aatttgggat
     2461 caatttgtac caaagttcag cagttaaaaa agaatcaaaa tctttgttaa cgcaaatgta
     2521 ttctgaagaa aatgaagttc tttttatcta aaaaaaggta gaaagtaaac ttttacatac
     2581 catcattgga aagtgtttaa attgaaactc tttccgatga tgtctttaat tctaaagaaa
     2641 tgccaaacta tttaatattt ggtaattcaa aaaaaatgta gttattttgc aacttaaagt
     2701 tcacaaacgt attgaaatgt tataaagaaa gctcgaggga atagacccga tgaaagctta
     2761 gcaacccttt gccaacgaag aaggtgctac attctatccc gatttattgg gaaaagataa
     2821 caacaagaat ctagacactt ctagttttct ttctaatatt tccaaacaca aatcaaaaaa
     2881 atctaaaaag atttgaaaat tggaaaataa accaacaaca attaaaatac aaaatttcac
     2941 caccgaaagt ggcgtgctaa ttccttccat aaacttaagt tatcaggttt ttggtcaagt
     3001 gttgaatacc gctcctattg ttttggtgaa tcacgccttg accggaaatt cccaagtagt
     3061 gggcgaaaac ggttggtgga ataatcttat tggtgacaac aaaaccatcg atactaataa
     3121 atattctatt ctagctttta acgtgccagg caatggattt gataagacat acatcgaaaa
     3181 ttacttggat tttacagctc gcgatgtggc taggcttttc ttagaaggca ttcgatttct
     3241 aaaagtgaaa gaattatttg cactcatcgg cggttctgtt ggtggcggaa ttgcttggga
     3301 aatgattgct ttggatccta aattaaccca taattttttc ccaattgctt ccgattggaa
     3361 atcgaccgat tggttgattg cgaattgcta tttgcaagaa aaaattcttt gcaattcgtc
     3421 taaacctatc gaagatgctc gtattcacgc aatgttgtgt tatagaacac cagaatcctt
     3481 caaagaaaaa tttcaaagaa attcaactga ttctttcgaa tcctttcaag tggaaagttg
     3541 gctgaatcat cacggcgaga aattgcaaaa aaggtttcag ctttcggctt ataaaatgat
     3601 gaatcagttg ctgaaaacta tcgatattac aagaggaaaa acttcttttg aagatttaat
     3661 ttctcaagta gatgctaata tttatatcat tggaataaat tcggatttgt tttttacggc
     3721 taatgaaaat aaggaaacct acaacgaaat taaaaaattc aaacacaatg ttttttacag
     3781 cgaaatcgaa tcgcaacacg gacacgatgc ttttttgatg gaatacgaac aattaaacaa
     3841 tttattggat gttgttttta aaaataaaaa aaataagatg aaaatattaa aatttggcgg
     3901 aaaatcatta gccaacggag agggaatcaa taaagttttt gatattatta tcgataaaaa
     3961 aagcaagggc gaaaacattg cagttgttgt ttcggcaaga ggaaatgcaa ccgatgaatt
     4021 ggaagatatt ttgaccattg cagccaaaaa tggcaattac aaaccgttgc tagaaagttt
     4081 taaaagctac cagcaagatg aatacaaggt tgatttttca gaagaatttg ccattttaga
     4141 caaacttttt gagggagtaa gtttgattgg tgattatagc gaaaaaataa aagaccaagt
     4201 tttgtcacaa ggcgaagtaa tttcggcaaa attaattacc gcagtcttga atgaaaaagg
     4261 gattgctgct cgttttgctg ataccagaga attaataaaa accgattcta attttgggga
     4321 tgcacaacct attgagcaaa tttctaagaa aaatgtaatc cattatttca aagaaaatgc
     4381 tgctgttgtt aacattgtta caggttttat cggttcaaat agcagcggtg aaaccaccac
     4441 tttagggaga aatggtagta attattcggc ctctttgatt gccaattatg tcgatgccga
     4501 agaattgcaa aactatacgc acgttgacgg aatttatact gccaatccag atttagttcc
     4561 cgatgccaaa aaaattgatc gtttgtcttt taacgaagcc aatgaaattg ccaattttgg
     4621 tgcaactatt ttacacgcca aaaccattat tccgttgttg gaaaaaaata ttccacttcg
     4681 aattttgaat actttcaatc acgaaaatca aggaacatta atcacatcca aatcgaataa
     4741 agaagggatt aaaacacttt cggtgctcga aaatgtagct ttggtcaatc ttgaaggaag
     4801 aggattgcta ggaaaaacag gtgttgacgc tcgaattttt aaagtaatgg gcgataatga
     4861 tattagcgta agcatcattt cgcaaggttc gtctgaaaga ggaattggac ttgtggtcaa
     4921 ctctaatcaa gccacaaaag caatgatcga attagaaaaa gaattcgaaa atgatttcta
     4981 ttcgaaagac gtaaacaaaa tttcaatcac cgatgatgtt tcggttattt cgataattgg
     5041 gcaggatttg agcacttttc acaaacctta cacggctttg attaaaaaca gaattgtgcc
     5101 aatccttttc aataatactg taacaggaaa aaatgtgagc ttggtggtca aaaaagcaca
     5161 attaaacaag gcgttgaacg tgattcacgg tgagattttt ggcgtttcta agaaaataaa
     5221 tatcgcagtt tttggtcacg gattggtggg aggaacattg attaatcaaa ttttagaatc
     5281 ggctccagct atcgaaaaaa ggaaagatat taaattgaat gtttttgcga ttgccaattc
     5341 taagaatttg cttttgaata aaaacggcgt ttcacccaat tggaaaaatg agattcaaac
     5401 caatggtttc tcctatacaa tagatgatgt tatcgctttt gccaatgcta ataatttaga
     5461 gaatttgatt gccattgaca acacggcaag tgcagcattt gttgaacatt ataccacttt
     5521 agcagaaaat agtttcgact tgatttcttc gaataaagtg gcgaatacct tgagctataa
     5581 attctataaa gatttgagaa aagtattggc cgaaaatcaa aagaattatt tatatgaaac
     5641 caatgtgggt gcaggtttac cattaattga taccatcaaa ttattgcatc tttcgggcga
     5701 aaatatcact aaaatcaaag gtgttttctc gggaactttg agttatttat tcaataattt
     5761 ttctgccaag gatgttccgt ttagtgaaat tttgaaagaa gcgattgata acggatacac
     5821 cgaacccgat ccaagagaag atttgtgtgg aaacgacgtg ggtcgaaaat tattaatttt
     5881 ggcaagagaa ttggatttgc aaaacgaatt tgaagaaatt agcattcaaa acctgattcc
     5941 agaacatttg cgtgaaggta acgttgccga tttcttgaat aaattaaaag aattcgaccc
     6001 gatttacaac aaaataaaag aagagcaaga accgaatcac gtgttgagat acatcggcga
     6061 attgtcgggc gatttacaaa acgacaaagg aaatctagaa gtaaaattag tttccgtacc
     6121 ttcggatacc gctttgggcg gattgaaagg ctccgattcc ttcttcgaaa tttacacaga
     6181 atcttacgga gacagaccaa ttgtaattca aggtgctggt gcaggatcgg ctgttacagc
     6241 gagaggagtt tttggtgatt tattaagatt gtcggagaaa gggtagagat ttaagattgt
     6301 agaatttaga tttaaataaa tataaaatcc gcgtttgaaa tccgtttcat tcgcgtccaa
     6361 aaaaataaaa aatgaaagta acattaaata gagtaaacga aaacttccac ttcgaattga
     6421 aaaacgaacg tggacatata gtaaatgtag atgcaagacc tgattttggg ggcaatgata
     6481 tgggaccaag tccaatggaa ttggttttaa tgggtgttgc aggttgcagt ggaatcgata
     6541 tgatttccat cttgaaaaaa caacgtcaag aaatcacttc tttcaaagcc gatgtagaag
     6601 gcgagcgtgt gcaagtggga gatgccaaac cattcaaaga tatttatgta gtattttatt
     6661 tggaaggaaa tatcaaagaa gacaaagcgt tgaaagccgc ccaattgtca ttcgaaaaat
     6721 attgctcggt tgccaaaaca gtggagccaa cagcaaccat acattataaa gtagttttga
     6781 acggaagccc attagaaaag atttaatcaa ttaaattatt agtttaaaga gtaaaagtcg
     6841 aaatgcagtt ttccaaccat tttaaacttt aaactttaaa ctttaaacaa aatagaatga
     6901 acgaacaaga atttggtttt gaaacccaag ccatacgcaa tcaattagag cgcacccaat
     6961 atttagaaca ttccgtgcct ttgtacttaa cttccagttt tgtatttgaa gatgccgaag
     7021 atatgcgtgc ctcttttgct gacgaaaaag atagaaatat ttacagtcgt tacagcaatc
     7081 ccaataccaa tgagtttgtg gataaagtgt gccaaatgga aggtgctgct tctggttttg
     7141 ctttcgcatc cggaatggcg gcggtctatt cgaccttggc ggctttgttg aattcgggtg
     7201 atcatatcgt ttcggcgggt agtgtatttg gtgcaacaca ctctttgttt gtgaattatt
     7261 ttccaaaatg gggaatagaa acatcgtatt tcgatattga caaaccagaa actattgaaa
     7321 gttttataaa accaaacacc aaaattcttt ttgccgaatc accaaccaat cctgctgttg
     7381 atattattga tttggaattg cttggagcta ttgccaaaaa acacaatttg attttgatta
     7441 tcgacaactg ttttgcaacg ccttatttgc aacaacccat aaaatgggga gcgcatttgg
     7501 tagtgcattc agcaacaaaa ttaatggacg gtcaaggtag agttttgggt ggaattacgg
     7561 ttggtgatgc cgaattgatt caaaaaatct atttgttttc gcgattgaca gggccttctt
     7621 tatcgccgtt taatgcttgg gtattgtcca aaagtttaga aactttggcc attcgtttgg
     7681 atagacattg cgaaaatgct ttgaaagtag ccgagttttt agaacagcat ccgaacgtaa
     7741 gtttggtaaa atatccgttc ttgaaatcgc accctcaata tgaaattgcc aaaaagcaaa
     7801 tgaaattggg aggcaacatt gtagctttcg aaatcaaagg tggcattgaa gccggaagag
     7861 ctttcttgaa taaaataaaa ttatgttcgt tgtcacccaa tttaggcgac accagaacca
     7921 ttgtcacaca tccagcctct acaacacaca gtaaattgtc tgtcgaagag cgtttggcag
     7981 taagtattac cgatggattg gtgcgtgttt ccgtaggatt ggaaaccgta gctgatatta
     8041 ttgcggattt ggagcaggcg ttgggttaaa aatttataat tattgattca tacataaata
     8101 gcccacggtt tttgatcgtg ggttatgtgg ttatagttaa aaacggaatt agtaattcat
     8161 caaatataaa taggttttta attttagaat gttgtaaatt tggtctttat cttacaattg
     8221 catggagagc tctcgaaaaa taatatcggt tttaatttta cttattttaa tttcaagttg
     8281 caaaaagaaa caaactgagt ttgaatttga aaaaagcgtt atgactgaaa tttttccaag
     8341 tttaattgag tctacttgta ttgattctag agttttggct aatcttcctc cagttggaaa
     8401 atcaatttat gataaaaacg gtaataatat tggtttagat acaaccaata taaagcgaga
     8461 aagagaaatt tatgaaagag aagaacttag aattaaaaat gacacttcga aaataatctt
     8521 tgcgtttgat tcaaaaatta aaagaaatag aaataatgtt gaagaagatt ttgaaaaaca
     8581 tttttcagga gcaaaaattt ttgaatctaa aaaagaacaa aatttagaat acgattttga
     8641 tttcaaagaa ttaaaaataa aaaataaatt tgtcctggaa aatatttctg aattcccgaa
     8701 aggaaaaggt gaaatatgga agattttata gtcttttgta tggaagctgt attgtnnnnn
     8761 nnnnnatgga aggcaaaata taattttatt ttttcgggtg tagttttctt tactagaata
     8821 caatttgata aagataaaaa gtttggaatt cttgatggtg gatttgcttg tggaaggctt
     8881 tgtggccaag gttctagaat atacattaag aagataaata acaaatgggt aattgataaa
     8941 attgaaggaa cttgggtttc ataaaaaact tcgtatatct aatctcgtaa atcaaacatc
     9001 aaaaatgctc tcaaaaaaaa caaaatacgg aatcaaagca cttactttct tggctcgc
//
LOCUS       DAOSNN010000090         9046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7655_length_9046_cov_4.427984, whole genome shotgun sequence.
ACCESSION   DAOSNN010000090 DAOSNN010000000
VERSION     DAOSNN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7655_length_9046_cov_4.427984"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="PLQ70_06055"
     CDS             complement(<1..1555)
                     /locus_tag="PLQ70_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQE34215.1"
                     /translation="MKYFIIVFLSTFVFSSKGFSQEKNTTHKVEKGETIGQIAQKYKV
                     TPYDIYKLNPDAQSGLKPNSILLIPKKGETPKLNSTIKTHKVEPKETLFGIENKYGVT
                     DEALKKANPDLEKLGLQIGQTLVIPSNTVSKTVTSTPEKVVYHEVLPKETKFSIAKQY
                     GITIEELEKRNPEIIPNLTIGYKLIVKGNPPKTDKVVVAEPKKETVKPITSNSIMQQE
                     MEPKKNYATLEVKNGTNDRKKLVLLLPFNLNKIEGDTVNSTAMRLRKDKFLNMTLDFY
                     SGALMAIDSARSIGIPVDVSIFDSQETKNSSNISNINQQQNLESADAIIGPFYQNNVE
                     KTAALVSTKNVAVISPLSKDIGNSFPNLYQTIPSAEVLKRAVFEYMIAKNGNIIAVVD
                     KKKESARQFIQQNYSQVQFAALLENGSLSAESLKGLLVKDKVNYVVLASENTGMIKWT
                     MSTMMSVMAKYNVQLVIMEPNETLDTDEISFENLTKLKLMYPSISRENESTEALIFEK
                     EYRKKNKIAP"
     gene            complement(1662..3191)
                     /gene="guaA"
                     /locus_tag="PLQ70_06060"
     CDS             complement(1662..3191)
                     /gene="guaA"
                     /locus_tag="PLQ70_06060"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQE34216.1"
                     /translation="MQHNVLILDFGSQYTQLIARRVRELNIFCEIFPYNHFPDDLSSY
                     KAVILGGSPFSVRGEDAPHPDLSQIRGKLPMLAVCYGAQYLAHFSGGEVAASNTREYG
                     RANLSFIKEDETFFEGVSENSQVWMSHSDSIKALPTNGVKLASTHDVEFAAYKIEGET
                     TYAIQYHPEVFHSTDGSKMLENFLVKIAHVPQNFTPNAFVEEIVAEMKEKIGNDKVVL
                     GLSGGVDSTVAAVLLNKAIGKNLYCIFVNNGLLRKNEFQSVLDQYEGMGLNVKGVDAS
                     QRFYDALAGVTDPELKRKAIGNAFIEVFDDESHKIEDVTWLAQGTIYPDVIESVSVKG
                     PSATIKSHHNVGGLPDYMKLKVVEPLRMLFKDEVRRVGATLGIDPELLGRHPFPGPGL
                     SIRILGDITLEKVQILQDVDKVFIDGLKSWGLYDQVWQAGAILLPVNSVGVMGDERTY
                     EKVVALRAVQSTDGMTADWVHLPYEFLMKVSNDIINKVKGVNRVVYDISSKPPATIEW
                     E"
     gene            complement(3345..4013)
                     /locus_tag="PLQ70_06065"
     CDS             complement(3345..4013)
                     /locus_tag="PLQ70_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34217.1"
                     /translation="MKRILFFIIMIGLGSNAFGQIDLNTKFKPIKIKNVKPKKEVPPP
                     ANLPKLDLPKIVAPNVFKETNIYGTKPQVNNSFEIGTAENHFSMTLTNKYKHKLGDVY
                     QEKMTQDLDKTMIREGLKEDSSLLDREDRYLGDFKTKSNELVVKYRDYIQIDGDLINV
                     YLNDQLIQSRLYLYSNLHEFKIPLTTGLNKIEFVVASQGTSGGNTAEIHVIDGSNKSV
                     TDEY"
     gene            complement(4076..4756)
                     /locus_tag="PLQ70_06070"
     CDS             complement(4076..4756)
                     /locus_tag="PLQ70_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34218.1"
                     /translation="MKQFLLFIFIIGIASKSFGQAEFNSKFKPIPPINIKPKPKKETV
                     PPPTDLPKIVAPNILKNTNIFNTKPKVDNSFQIGIPENKFTMTPTNTFVNPGDKVLNR
                     LNRKADNEDQTVYRRNQDLGSYRTKSLIAKLSYRDYGEVDGDKIRVLLNDKTLATEVY
                     LNSDFQGFEITLVDGINKIDFEALNQGQLGPNTAEFRIYDDAGGLISASQWNLGTGFK
                     ATIIITKE"
     gene            5019..5405
                     /locus_tag="PLQ70_06075"
     CDS             5019..5405
                     /locus_tag="PLQ70_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQE34219.1"
                     /translation="MKRENILTGSPWEDKMGYCRAVRIGNIIEVSGTVAIVDGEKVKA
                     DDAFAQTINILERIEKVLEDLNVGMKDVIRTRIFTTEITTFEDVARAHATFFKDIKPA
                     TGFYEVSKLVAPEYLVEIEFTAIAAE"
     gene            5522..6769
                     /locus_tag="PLQ70_06080"
     CDS             5522..6769
                     /locus_tag="PLQ70_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HQE34220.1"
                     /translation="MNLKNTILLSFKWIFICVLIGIFSGSASAFFLVSLEWVTQFREH
                     NNWIIWLLPVGGLYIGWLYYKYGTTVVKGNNLLLEEYENPQKTIPFRMAPMVLISTLI
                     THLFGGSAGREGTAVQMSGAIADQFTTYFKLDNSDRRTLIILGISAGFASVFGTPLAG
                     AIFALEVLYFSKINLKSSVLSFVVAYIAYFTVEILQVKHTHYSIPEVPEISINVIWYS
                     IAVGIVFGLAAMIFSRTTHFWGRLFAKTIEYGPLRPFVGGLILVIAIYLMGTTKFIGL
                     GIPSIVESFSTANTPYDFLLKILFTGFTLGAGFKGGEVTPLFFVGATLGSALSVVVPL
                     PIALLAGMGFVAVFSGATHTPVACTVMGMELFGIESGVFIGIACLVAYFASGSVGIYH
                     SQIVKGAKYRLYQRFTRKDLEVF"
     gene            6980..7984
                     /locus_tag="PLQ70_06085"
     CDS             6980..7984
                     /locus_tag="PLQ70_06085"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HQE34221.1"
                     /translation="MKEQDIQAIEQLLATPKKIAIIPHRSPDGDAMGSTLGLYHFLLK
                     NNHQPVVVSPNEFPDFLAWMPGSETVKIFEKDKKNCTQILEEAEIVFTLDFNALHRVG
                     EMEKVLENLTAPFVMIDHHQSPDDYATVTYSDTAFGSTCEMLYHFISFLGKKSDIDKT
                     IGTCIYTGILTDSGSFRFPKTTGTTHRIIADLIDLGVENSEIPALLYDNSSYDRLQLL
                     GRALQNMKIFPEHKTTYTTLTQAELDQFNHIKGDTEGIVNYGLSVKGIVFTAIFIENT
                     EEKIIKISLRSQGDFDVNLFAREHFNGGGHRNAAGGKSETSMQETVQKFEDLVSKLII
                     "
     gene            7991..8656
                     /gene="gldI"
                     /locus_tag="PLQ70_06090"
     CDS             7991..8656
                     /gene="gldI"
                     /locus_tag="PLQ70_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated peptidyl-prolyl
                     isomerase GldI"
                     /protein_id="HQE34222.1"
                     /translation="MNYRKLIVLAFLATLFITSCKQSQEARKPVSQASGSFMKKSAER
                     NKKLIASEEGRIDSLIKSNPKIKYLASTKGYWYTYITANTIDTLTPKKGDVAFFDYEI
                     KDLQGRIIYSEVELKPQTYAVDKQNIMTGLREGIKIMHKNEKITFLFPSHIAYGYHGD
                     TKKIGTNQPLLCTVTLHNFISEEAFKKEILLRATNAANPTPNQETVNRTKSVNKIKDS
                     LTN"
     gene            8693..>9046
                     /locus_tag="PLQ70_06095"
     CDS             8693..>9046
                     /locus_tag="PLQ70_06095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQE34223.1"
                     /translation="MKKSILYAFAFLVFVTLFSCKESKNNLPDGLYAQIETNKGTIIV
                     QLNYEKAPITVANFVTLAEGKNEFITNENLKNRPFYDGLKFHRVIPEFMIQGGDPLGT
                     GSGDAGYRFKDEITDL"
BASE COUNT         2876 a         1647 c         1493 g         3030 t
ORIGIN      
        1 ttggagcaat tttgtttttc tttctatact ctttttcaaa aatgagtgcc tcggtcgatt
       61 cgttttcacg acttatcgaa ggatacatca atttcaattt ggtcaaattc tcaaaactga
      121 tttcatccgt atctaaggtt tcgttgggtt ccataataac caactgcaca ttatattttg
      181 ccataacact catcatcgtt gacatagtcc atttaatcat tcctgtgttt tcagaagcca
      241 agactacata attcactttg tcttttacta gtaaaccttt caaactttct gctgataaac
      301 ttccattttc aagtaaagcg gcaaattgaa cctgactata attttgctga ataaattgtc
      361 tggccgattc ctttttctta tcgacaacgg ctatgatatt tccatttttt gcgatcatat
      421 attcgaaaac agctcttttc aaaacttcgg ctgaagggat ggtttggtac agattaggaa
      481 acgaatttcc aatgtctttg gacaaaggcg aaattacagc aacattctta gtacttacta
      541 aagcagctgt tttttcgaca ttattttgat agaaaggacc aattatagca tcggcagatt
      601 ctagattttg ttgctgattg atgttcgaaa tattggaact atttttagtt tcttgcgaat
      661 cgaaaatact aacatcaaca ggaattccga tgctacgagc agaatcaatg gccattaatg
      721 ctcccgaata aaaatccaaa gtcatattca aaaacttatc ttttctcaaa cgcattgcgg
      781 tagaattgac tgtatcacct tcaattttat ttaaattaaa aggcaataaa agcactaatt
      841 ttttcctgtc atttgtacca ttttttactt ccaaagtggc ataatttttc tttggttcca
      901 tttcctgctg catgatacta ttggaagtga taggttttac cgtttctttt ttaggttctg
      961 ccacaacaac tttatctgtt tttggcggat ttcctttgac aatcaatttg tatcctatgg
     1021 ttaaattcgg aataatttcg ggattccttt tttctaattc ttcaatggta attccatatt
     1081 gtttggcaat agaaaatttt gtctctttag gaaggacttc gtgataaact actttttctg
     1141 gagttgaagt cactgttttt gaaacagtat tcgaaggaat aacaagggtt tgtcctattt
     1201 gtaaacctaa cttttctaaa tcgggattgg ctttttttaa ggcttcatca gtaacaccat
     1261 atttattttc gattccaaat aaggtttctt tgggttcaac tttatgggtt ttgattgtag
     1321 aatttaattt tggagtttcc ccttttttag gaattagcaa aatgctattg ggtttcaaac
     1381 cactttgagc atcgggattt aatttataaa tatcatacgg agtaaccttg tatttttggg
     1441 caatttggcc aattgtttcg cctttttcaa ctttgtgagt tgtgtttttt tcttgtgaaa
     1501 acccctttga agaaaaaaca aaagtgctca aaaatactat gataaaatat ttcatctgat
     1561 tgatattgta taaatttagt ttttcaaaga taacaaaacg gtatcaaatt ttattccaaa
     1621 atctgatacc gcttttatat aatttccatt tttttaatgg attattccca ttcaatagtt
     1681 gctggcggtt ttgagctgat atcataaacc actctgttca cacctttcac tttattgata
     1741 atatcattag acactttcat caagaattca taaggcaaat gtacccaatc agcggtcatt
     1801 ccgtctgtag attgaacggc acgaagcgcc actacttttt cgtaagtacg ctcatcgccc
     1861 attactccta cgctattcac aggaagcaaa atcgctcctg cttgccaaac ttggtcatac
     1921 aatccccaag attttaatcc gtcgataaaa actttatcta catcttgtaa gatttgcact
     1981 ttttctaatg taatatctcc taaaatacga atagacaatc caggtcctgg gaaaggatgt
     2041 cttcctaata attcgggatc gattcctaaa gtagccccta ctctacgcac ttcgtctttg
     2101 aaaagcattc gcaaaggttc tactactttc aatttcatat aatcaggcaa accacccaca
     2161 ttatgatgcg atttaatggt tgcagatggt ccttttactg aaactgattc gataacgtca
     2221 ggataaatag ttccttgagc taaccaagtt acatcttcaa ttttatgtga ctcatcatca
     2281 aaaacttcga taaaagcatt accaatagct tttcttttta attctggatc agtaactcca
     2341 gctaaagcat cataaaaacg ttgggaagca tccactcctt tcacgttcaa gcccattcct
     2401 tcatattgat ctaaaacaga ttggaattca tttttgcgaa gcaaaccgtt attaacgaaa
     2461 atacaatata ggtttttacc gattgctttg ttcaataaaa ccgctgccac tgttgaatct
     2521 actcctcctg aaagtcctaa aactactttg tcgtttccga ttttttcttt catctccgcc
     2581 acgatttctt ctacgaaagc atttggcgtg aaattttgag gaacatgagc aatttttact
     2641 aagaaattct ctagcatttt ggaaccatcc gtagagtgaa atacttcggg atggtattga
     2701 atcgcataag tggtttcacc ttcaattttg tatgctgcaa attctacatc gtgtgtacta
     2761 gcaagtttta cgccattagt aggcaaagct ttgatactgt cgctatggct catccaaact
     2821 tggctatttt cagaaacgcc ttcgaagaaa gtttcgtctt ctttaataaa agacaaatta
     2881 gctcttccgt attctcttgt attggaagcc gctacttcac caccactgaa atgggcaaga
     2941 tattgtgctc cgtaacaaac agcaagcata ggtaatttgc ctctaatttg ggataaatcg
     3001 gggtgtggtg catcttctcc tctaacagaa aaagggcttc caccaagaat taccgcttta
     3061 taactagaca aatcatctgg aaaatgattg tatggaaaaa tttcgcagaa tatatttaat
     3121 tcgcgaactc gtcgggcaat aagctgagta tattgcgatc cgaaatctaa aataagtacg
     3181 ttgtgttgca ttcggcaaaa atacttttta taattgggat tgaaaaatcg aaatttgaaa
     3241 aatttgtaac tatttagtaa ttagttttct taaatcatta taaagttagc caatcactcc
     3301 tttatgacaa tcaatttaat tttggttcct aaagctaaat tattctaata ttcatcagta
     3361 accgatttat tagaaccatc aatcacgtga atttcggcag tatttcctcc agaagttcct
     3421 tgacttgcta caacaaattc aattttattg agtcctgttg ttaaaggaat tttaaattcg
     3481 tgtaaattcg aatacaaata caaccgagat tgaatcaatt gatcattcaa atacacatta
     3541 atcaaatcgc cgtctatttg aatataatcg cggtatttca caaccagttc attcgatttt
     3601 gtcttaaaat ctcctagata gcgatcttct ctatccaaca aactcgaatc ttcttttaac
     3661 ccttcacgaa tcatagtttt atctaaatcc tgagtcattt tttcctgata aacatctccc
     3721 aatttatgtt tatatttatt ggttagagtc atcgaaaaat gattttcggc tgtccctatt
     3781 tcaaatgaat tattaacttg gggtttcgtt ccgtaaatat tcgtttcctt gaaaacatta
     3841 ggtgctacga ttttgggtaa atccaatttg ggcaaattag ctggaggagg aacttctttt
     3901 ttaggtttta cattttttat ttttatgggc ttgaatttgg tatttaaatc gatctgtcca
     3961 aaagcatttg agcccaaacc tatcataata ataaaaaaaa gaattcgttt catatcgttt
     4021 taaatagagg aatccataga tttagagatt ccaaaaatca caaaattata ttttattact
     4081 ctttagtaat aatgatagtc gccttaaaac cagtacccaa attccattga cttgccgata
     4141 tcaaaccacc cgcatcatca taaatccgaa attctgctgt attaggccct aactgacctt
     4201 gatttaatgc ttcaaaatca attttattga taccgtcaac aagcgtgatt tcaaaccctt
     4261 gaaaatcact attcaaatat acttcggtgg ccagtgtttt atcgtttagc aaaactctta
     4321 ttttgtcacc atccacttct ccataatcgc ggtagcttaa tttagcaata agcgatttag
     4381 ttctataact tcctaaatcc tgatttcttc tatagaccgt ttggtcttca ttatctgctt
     4441 ttctgttcaa tcgattcaac actttatcac ctggatttac aaaagtattg gtaggagtca
     4501 ttgtaaattt attttcagga attccaatct gaaatgaatt atcaactttg ggttttgtat
     4561 tgaaaatgtt ggtgtttttt agaatattag gagctacaat ttttggtaaa tcggttggcg
     4621 gaggaactgt ttctttcttg ggttttggtt ttatatttat aggtggaatg ggtttgaatt
     4681 tagaattaaa ttcagcttgc ccaaaacttt ttgaagctat tccaataata aaaatgaata
     4741 aaagaaactg tttcatattg ttttttatat tgatggctca atatttaaaa agttcaaaaa
     4801 taatagaaat ctaagagcaa aaatcacaaa aattacataa aaatttgctg tgattggatt
     4861 ttagaaatct gaatactttt aaagcctttg aaacaatttt tatgccataa tactagtgtt
     4921 tctatgattt cttgtccttt tttttataaa ttgcagcact aaaacaaatt caggagttgg
     4981 cattcaactc gttaaaaatc aacacaaaaa caattaaaat gaaaagagaa aacatcttga
     5041 caggttcgcc ttgggaagac aaaatgggat attgtcgtgc ggttcgcatt ggaaatatca
     5101 tcgaagtatc gggaactgtt gctattgtag atggcgaaaa agtgaaagct gatgacgctt
     5161 ttgcacaaac aataaacatt ttagaacgaa ttgaaaaagt tttagaagac cttaatgtag
     5221 gaatgaaaga tgtgattcgt actcgaattt tcaccaccga aattactact tttgaagacg
     5281 ttgctagagc ccacgctact tttttcaaag acatcaaacc agctactggt ttttatgaag
     5341 tgagcaaatt agtagctcca gaatatttag tagaaataga atttacggct attgctgctg
     5401 aataattttc tacaattttg tcattccgta ggaatctcaa caaacaagag attcctacgg
     5461 aatgacaatt aaaccttttt attctagtaa aaggcaaaat ctaattaaag tactgaattt
     5521 aatgaaccta aaaaacacaa tccttctttc cttcaaatgg attttcattt gcgttctgat
     5581 aggtattttt tcgggttctg cttcggcttt tttcttagta tccttagaat gggtaaccca
     5641 attcagagag cacaacaatt ggatcatttg gctcttgcct gtgggcggac tttatatcgg
     5701 ttggctgtac tataaatacg gaacaactgt tgtaaaaggc aataatttat tgctagagga
     5761 atatgaaaat cctcaaaaaa caatcccatt caggatggct ccaatggtgc ttattagcac
     5821 tttaatcact cacctttttg gaggctcggc gggtcgagaa ggcacagcag ttcaaatgag
     5881 tggagctatt gccgatcaat tcactactta ttttaaactt gacaattccg accgaagaac
     5941 actaatcatt cttggtatta gcgctggttt tgcctcggtt tttggaacgc ctttagcggg
     6001 agctatcttc gctttagaag ttttatattt tagtaaaatc aatttaaaga gtagtgtact
     6061 ttcgtttgta gtggcttata ttgcttattt tacggtcgaa attctacaag taaaacatac
     6121 gcattattcg attcccgaag ttccagaaat tagcattaat gtcatttggt atagcattgc
     6181 tgttggaatt gtatttggtt tagcagctat gattttttct cgaaccactc atttttgggg
     6241 acgtttattt gcaaaaacta tcgaatatgg tccactgcgc ccgtttgtgg gtggactcat
     6301 tttggtcatt gccatatact taatgggaac cacaaaattc attggattag gaattcctag
     6361 cattgttgaa tccttttcaa ctgcaaatac tccttatgat tttctcctca aaatactatt
     6421 tactggtttt acgctcggag ctggattcaa aggtggcgaa gtaactcctc ttttctttgt
     6481 aggtgccaca cttggaagtg ctttatctgt cgttgtcccc ttgcctattg cactattggc
     6541 gggaatggga tttgtagccg tattttcggg agcaactcac actcctgttg cttgcactgt
     6601 tatgggaatg gaactttttg gtatcgaaag tggcgttttt attggcattg cttgcttggt
     6661 agcttatttc gcatctggtt ctgtgggaat ctatcattcg cagattgtaa aaggagcaaa
     6721 atatcgtttg tatcagcgtt tcacacgaaa agatttagag gttttttaaa gaatttcagc
     6781 taaaaaacca ctcctcgtat tctttaacaa taatttaata gttacggatg gtcatttggg
     6841 atttcctata ttgcatttac attaaaaaaa tgtaatttcg caaactatga aatcgccaca
     6901 aaatagaaat ttgcctaagc aaacaccaat aaaaaaaggc gataccatat gtgaccgctg
     6961 aaaaatataa tttacacata tgaaagaaca agacatacaa gctattgaac agttattagc
     7021 cactccaaaa aagattgcaa tcattccgca tcgaagccct gatggagatg caatgggttc
     7081 aacccttgga ttatatcatt ttttattaaa aaacaatcac caacctgtgg tggtttcacc
     7141 caacgaattt cctgattttt tagcttggat gccaggctca gaaacggtca agatattcga
     7201 aaaagataaa aaaaattgca cccaaattct ggaagaagcc gaaatagttt tcaccttaga
     7261 ttttaatgct ttgcatcgtg taggcgaaat ggaaaaagta ctcgaaaatc taacggctcc
     7321 tttcgtgatg atagatcacc atcaatcgcc agatgattat gcaacagtta cttattcgga
     7381 tacagctttt ggttctactt gcgaaatgtt gtatcatttc atttcctttc ttggcaaaaa
     7441 atcagacatt gacaaaacca ttggtacttg tatttataca ggaattctaa ccgattcggg
     7501 ctcttttcga ttcccaaaaa ccactggaac aactcataga atcattgccg atttgatcga
     7561 tttaggtgtc gaaaacagtg aaattccagc actcctttac gataatagtt cgtatgacag
     7621 attgcaatta ttaggaagag cacttcaaaa tatgaaaatt tttccggagc ataaaactac
     7681 ttataccaca ctaacacaag cagaattaga ccaattcaac cacataaaag gcgataccga
     7741 aggcattgtt aattatggct tgagtgtaaa aggaattgtt tttaccgcta tttttatcga
     7801 aaataccgaa gaaaaaatca tcaaaatttc attacgctcc caaggcgatt ttgatgtcaa
     7861 tctttttgca agagaacatt ttaatggcgg tggacatcgc aatgcagctg gaggaaaatc
     7921 agaaacttcg atgcaagaaa cagtacaaaa atttgaagat ttggtaagca aactaataat
     7981 ataacgcatt atgaactatc gcaaacttat tgtattggca tttttggcaa ctctttttat
     8041 aactagttgc aagcaaagtc aagaagctag aaaacctgtt tcgcaagctt cgggatcatt
     8101 tatgaaaaaa tcggccgaaa gaaacaaaaa gctcattgct agcgaagaag gtcgaataga
     8161 ctctctaata aaaagcaatc ccaaaataaa atacttggct tcgaccaaag gatattggta
     8221 cacctacata acggctaata caattgacac cttaactccc aaaaaaggag atgttgcctt
     8281 ttttgattat gaaatcaaag atttacaggg tagaatcatc tattcggaag ttgaattaaa
     8341 accacaaacc tatgctgtcg ataaacaaaa tattatgaca ggattgcgag aagggattaa
     8401 aatcatgcat aaaaacgaaa aaataacttt tctgtttcca tcccatattg cttatggata
     8461 ccacggcgat actaagaaaa taggaacaaa ccaacccttg ctttgcaccg ttactttaca
     8521 caattttatc tctgaagaag ctttcaaaaa agaaattcta ctcagagcaa ccaacgctgc
     8581 aaacccaact cccaatcaag agaccgtaaa tcgaacaaaa tctgtaaata aaatcaaaga
     8641 cagtttaacc aattaatttt aagacatcaa taaaaacaaa tttaaaattc atatgaaaaa
     8701 aagcattcta tacgcatttg cctttctagt ttttgttact ttattttctt gcaaagagtc
     8761 taaaaacaat cttccagacg gattgtatgc ccaaatcgaa accaataaag gaactatcat
     8821 cgtacaattg aattatgaaa aagccccaat cacagttgct aattttgtga cattggccga
     8881 agggaaaaat gaattcatta ccaacgaaaa tttaaaaaac cgaccttttt atgatggatt
     8941 gaaatttcac cgtgtcattc ccgaatttat gatccaaggt ggcgatccat taggaaccgg
     9001 ttctggggat gctggctata gatttaaaga cgaaattacc gatttg
//
LOCUS       DAOSNN010000091         8950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7767_length_8950_cov_5.072625, whole genome shotgun sequence.
ACCESSION   DAOSNN010000091 DAOSNN010000000
VERSION     DAOSNN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7767_length_8950_cov_5.072625"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..2682)
                     /locus_tag="PLQ70_06100"
     CDS             complement(97..2682)
                     /locus_tag="PLQ70_06100"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HQE34224.1"
                     /translation="MYTHPKIERFNEEVLSKYHIYNSVFITLPFDSIDNTGVLLPLFT
                     DGCENGFKKQKTPKEIVDFFANKYMQGATESEKIDIMFRFIQFIERQIVLFDAIEDAA
                     FPVVNNMDGRGSLRDIKEKAAVKELGEELISFLENFNVRTVLTAHPTQFYPGSVLGII
                     NDLTDAIRKNNLLEIKQLLAQLGKTPFIQNEKPNPFDEAVSLIWYLENVFYETAGEIV
                     HYLQKNILHGSSINNQLIKLGFWPGGDRDGNPFVTTEITLKVADRLRTSILKCYYREM
                     RNLKRKLTFFEVDVLVAELEHKLYRSVFYSKGEIYITLEEFKSQLNKIRTILIERNQS
                     LYLDELEGLLIKVNLFGFHFATLDIRQNSKIHDAVFKDVVQHYLNSNSTVFPANYFDL
                     SEDEKFEILSNINGNLDSNVFDNEITRLTLESIQAIKQIQENNGESGANRYIISNNES
                     ALNVMETFALFKLNNWENLSVDIIPLFESVDDLQNAHNIMEKLYSNPAYAAHLVNRNK
                     KQTIMLGFSDGTKDGGYLMANWSIYKAKEELTEMSRKYGIKVIFFDGRGGPPARGGGK
                     THKFYASLGPKIENKEIQITVQGQTISSNFGILDSCRYNLENLLSAGATNQVFTKDKN
                     LLSVDEKQILDQLAELGYQKYLSFKNHPKFIPYLEKMSTLKYYAKTNIGSRPSKRSKS
                     EHLDFADLRAIPFVGSWSQLKQNVPGFFGVGSALKHFEETNSWNKVQDLYNGSLFFKT
                     LLENSMMSLAKSFFPLTAYMKNDPEFGEFWQIIYDEFLETKRLLLKIAGHKELMENYP
                     DGKASIDIRERIVLPLLTIQQYALLRINELSKAEKPDLELIQTYEKIVTRSLFGNTNA
                     SRNSA"
     gene            complement(2783..3262)
                     /locus_tag="PLQ70_06105"
     CDS             complement(2783..3262)
                     /locus_tag="PLQ70_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HQE34225.1"
                     /translation="MSKFRLDEVDHQILDMLIDNTRIPFTDIAKKLLISAGTVHVRVK
                     KMEDAEIIMGSSLVLDYDKLGYSFIAYVGVFLNNTSQTKFVLQRINEIPFVTVASVTT
                     GKFNIFCKIRAKDTKHAKDVIFMIDDIDGVYRTETMISLEESINDKKRLMHTIFKDI"
     gene            complement(3293..4450)
                     /locus_tag="PLQ70_06110"
     CDS             complement(3293..4450)
                     /locus_tag="PLQ70_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HQE34226.1"
                     /translation="MDLEQLFGQYKEQSIEGRYITLDKIYPILEKLNTNNQLEIIGKS
                     VLDIPIYKFQIGTGKIKILLWSQMHGNESTTTKALFDFLNLLSGDSELAKTLLKAFTF
                     YAIPMLNPDGAKLYTRANANEVDLNRDSQDLTQPESRVLREAFECFKPDFCFNLHDQR
                     TIFGVASTGKPATLSFLAPAYNDEREVNETRLKAINLIAGINDVLQNYIPGQVGRFDD
                     GFNINCIGDTFQFLGVPTVLFEAGHFQNDYWREETRKYVFMALVSSFEILSENDIVDS
                     RFDNYLSIPQNKVVFYDFLYKNIKINYDGIEIITNFAAQYKEELINGQICFNAYIVQI
                     SELENYFGHFEYDAKGAIFQGENDNSPKINQKANFYLNDKKFVNGVLVQSL"
     gene            4762..5241
                     /locus_tag="PLQ70_06115"
     CDS             4762..5241
                     /locus_tag="PLQ70_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQE34227.1"
                     /translation="MVNTDDFIKRIEIILDYYGLNASSFADKIGVQRSSLSHLLSGRN
                     KPSLDFILKILDVFPEVDLYWMLNGKGNFPKSELAIESNSTSPTPILNTQTAASEIVK
                     QPDLFSEKLVLEKNEKLENQFPRFSNSKTAIIPKTEGEIDRIVIFYTNGTFKTYSAE"
     gene            complement(5243..5800)
                     /locus_tag="PLQ70_06120"
     CDS             complement(5243..5800)
                     /locus_tag="PLQ70_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQE34228.1"
                     /translation="MKKSFYKLIFFQWMGWKIEGTIDESIKKCVLMVIPHTSWHDFYL
                     GLFTRGIIGIQMNFIGKKELFRFPFGFYFHWMGGAPIDRVGNLNKVEAIAKIFETRDE
                     FRLAISPEGTRKKVKELRTGFYYIALKANVPIIPVAFDFGQKSVKMGRPFFPTSNYEK
                     DLPILLQHFNGVVGKIPENGFSQEK"
     gene            5862..6524
                     /locus_tag="PLQ70_06125"
     CDS             5862..6524
                     /locus_tag="PLQ70_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQE34229.1"
                     /translation="MLKKLFSYLLPVTIFKQNSTLSKTLEVTWTDGKLVLDSENTNYS
                     YGNLQRILRKGLKIIGFEKIKSMQKILVLGVAGGSVIRTLVDEIKFEGQITGVEIDKN
                     IIDIANKYFQLNEIPNLEIIFDDASKFVLKTTETFDLIIIDVFQDTKMPDFLFEFFFI
                     DRICFLLNPKGHIMFNTMLLNQQHREFNLNYVKNFDPKKYTVTLYNKMEKTNELILIE
                     SK"
     gene            6594..7304
                     /locus_tag="PLQ70_06130"
     CDS             6594..7304
                     /locus_tag="PLQ70_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQE34230.1"
                     /translation="MKSILKKLFLGNLAKMPKPEFNPVQKRLMNIKAIWNNNHQDDNG
                     IEKFVRLFLSSSQLLFPGVYIKYLAYLRGYEYRDLALDFYVLLKVSFPLFILMNHWQS
                     YHFVIWIMIYVLLETILYIPTLIFASDMFSKPRSYKRSMLLLFLNYIEIIFSFGVLYS
                     CGDYLNKPLDHWFDPIYFSTINSASIGYGDFYPVTPIGKFLTTAQAFLFLLFVVLFLN
                     FFTAKIKVKGYFDIENES"
     gene            complement(7306..8031)
                     /gene="kdsB"
                     /locus_tag="PLQ70_06135"
     CDS             complement(7306..8031)
                     /gene="kdsB"
                     /locus_tag="PLQ70_06135"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQE34231.1"
                     /translation="MKIIAVIPARYASTRFPAKLMQDLGGKTVILRTYEAAQQSHLFD
                     DVFVVTDSDLIFDEIVSNGGKAIKSIKEHESGSDRIAEAVENLNVDIVVNVQGDEPFI
                     NTVALEKLIEVYKNDTEKKVDLASLMSEIKDEAEINNPNNVKVVVNQNGFALYFSRSV
                     IPYPREANVGVRYMKHIGIYAFRKQALMDFYNLPMKSLEASEKLEQLRYLEFGKSIKM
                     VETTHIGIGIDTKEDLEKARRLI"
     gene            complement(8034..8447)
                     /locus_tag="PLQ70_06140"
     CDS             complement(8034..8447)
                     /locus_tag="PLQ70_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34232.1"
                     /translation="MIKRNIFIVIIFLVSLALFFYSKRIDNGRLNEYLGNYKYSVGEI
                     EVYFERSTIGFNVGKSSITYSYIVNNKKIINNYDLLFYKLPENPKKGNKYIVLYNENE
                     PQKSILLGNYKIENNLEFKKYIEEFKNNPPDYRNK"
     gene            complement(8528..>8950)
                     /locus_tag="PLQ70_06145"
     CDS             complement(8528..>8950)
                     /locus_tag="PLQ70_06145"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HQE34233.1"
                     /translation="EANVPVIQLSIDYTKSPQYHFELAKKLNDLRRKGILIIGSGNIV
                     HNLRLVDFPNFDKDNYGYDWAIEARETVNNYLLDGNFQPLIDYEKQSKALQIAIPTPD
                     HYLPLIYTLGLKDKTEELSLFNDKLVAGSLSMTSVKIV"
BASE COUNT         2943 a         1553 c         1240 g         3214 t
ORIGIN      
        1 ttcaacattc tcaattgctt tgatataagt agcattgaaa gtcgaatatt catttacggg
       61 taattgattg gaattcatat tgtcgatttt tataaattaa gctgaatttc tactggcatt
      121 agtatttcca aaaagagaac gagttacaat tttttcataa gtttgaatca actctaaatc
      181 tggtttttcg gctttactca attcattaat tctcaataaa gcatattgtt gtattgtcaa
      241 caatggcaat actatacgct ctcttatatc aatagaagct tttccatcag gatagttttc
      301 cattagttct ttatgaccag caattttcaa taataatctt ttggtttcca aaaattcatc
      361 atatataatt tgccaaaact caccgaattc aggatcattt ttcatataag ctgtcaaagg
      421 aaaaaatgat tttgccaaag acatcatact gttttctaac aaggttttaa agaacaatga
      481 gccattatat aaatcctgaa ccttattcca actattggtt tcttcaaaat gtttcaaggc
      541 agagcccact ccaaaaaacc ctggaacgtt ttgcttcaac tgactccaag aacccacaaa
      601 aggaatcgct ctcaaatcgg caaaatctaa atgttctgat ttgcttcttt tagacggacg
      661 acttccaata ttggttttag cataatattt caaagtactc attttctcca aataaggaat
      721 gaatttggga tgattcttga aacttaaata cttttgatac cctaattcag ccaattgatc
      781 taaaatttgt ttttcatcaa ccgacaaaag atttttatct ttagtaaata cctgatttgt
      841 tgcaccagca cttaataaat tctctaaatt ataacgacaa gagtctaaaa ttccaaaatt
      901 agaactaatt gtttgtcctt gaacagtaat ttgaatttct ttattctcga tttttggacc
      961 taaagaagca taaaatttat gtgtttttcc gcctccacga gctggtggac caccacgtcc
     1021 atcaaagaaa ataactttaa ttccatattt tcgtgacatt tcggtcaact cttctttggc
     1081 tttataaata ctccaattcg ccatcaaata cccaccatct ttagttccat cagaaaaacc
     1141 aagcatgatg gtttgttttt tatttctatt aactaaatgg gctgcataag ctggatttga
     1201 atataatttt tccatgatat tatgagcatt ttgcaaatca tcaacagatt cgaaaagcgg
     1261 aataatatca actgaaagat tctcccaatt gtttaatttg aaaagagcaa aagtttccat
     1321 cacattcaat gcactttcgt tattgcttat gatgtaacga ttagcacctg attcgccgtt
     1381 gttttcttga atttgtttga tggcttgaat cgactcaagt gtcaatcttg taatttcatt
     1441 atcgaataca ttcgaatcta gattaccatt aatatttgac aatatctcaa acttttcatc
     1501 ttcgcttaaa tcaaaataat ttgctggaaa aacagtagag tttgagttta aataatgctg
     1561 tacaacatct ttaaaaacag catcatgtat tttactattt tgacgaatat ctaatgtggc
     1621 aaaatgaaat ccaaacaaat tcactttgat aagaagacct tctaattcat ctaaatataa
     1681 agattgattt cgctctataa gtatggttct aatcttattt aattgagatt taaactcctc
     1741 taaagtgatg taaatttctc ctttagaata aaataccgag cgatataatt tgtgttccaa
     1801 ttcggccact aaaacatcca cttcaaagaa agtaagcttt cttttcaagt ttctcatttc
     1861 acgatagtaa cacttcaata tagaagttct taatctatcg gcaactttaa gagttatttc
     1921 agtggtaaca aatgggtttc catcacgatc tcctcctggc caaaatccaa gttttatcaa
     1981 ttgattattg atagaacttc cgtgcaaaat atttttttgt agataatgaa ctatctctcc
     2041 agcggtttcg taaaacacat tttcaagata ccatatcaaa cttactgctt catcaaaagg
     2101 atttggtttt tcgttttgta taaaaggagt ttttcctaat tgagccaaaa gctgtttgat
     2161 ttcaagcaag ttgtttttgc gaattgcatc agtcaaatca ttaatgatac ctaaaaccga
     2221 accaggataa aactgagttg gatgagccgt taaaaccgtt ctcacattga agttttcgag
     2281 aaatgaaatt agctcctctc ctaactcttt taccgctgcc ttttccttaa tatctcttaa
     2341 cgaacctcta ccgtccatat tatttacaac cggaaaagca gcatcctcaa tagcatcaaa
     2401 taaaactatt tgacgttcga taaattgaat aaatcgaaac attatatcaa ttttctctga
     2461 ttctgttgct ccttgcatat acttatttgc aaaaaaatca acaatttctt taggtgtttt
     2521 ttgttttttg aaaccgtttt cgcaaccatc agtaaataaa ggaagtaaaa cccctgtatt
     2581 atctattgag tcgaatggca atgtaataaa tacactatta tagatgtgat attttgataa
     2641 aacttcttcg ttaaatcttt caattttagg gtgcgtatac atattgagtt ggtttagtta
     2701 ttgttggttt cgacagtttt tatataaaaa gaaaccccaa gaattcactt gaggttacgt
     2761 tatataacgg caatcaaatg aattatatat ctttaaaaat agtatgcatc aaacgttttt
     2821 tatcgttgat actttcttct aaagaaatca ttgtttctgt tctgtaaact ccgtcgatat
     2881 catcgatcat aaagattaca tcttttgcgt gcttggtgtc ttttgctcta attttacaaa
     2941 agatattgaa ttttccagtc gttacagatg ctactgtaac aaatggaatt tcatttatac
     3001 gctgtaaaac aaatttagtt tgcgaagtgt tattaagaaa aactccaaca taagcgataa
     3061 aagaataccc tagcttatca taatccaata ctaatgatga acccattatt atctcagcat
     3121 cttccatttt cttaactctg acgtgaactg ttccagccga aatcaataat tttttggcga
     3181 tatctgtaaa tggaattctt gtgttgtcaa tcaacatgtc caaaatttga tggtctactt
     3241 catctaaacg aaacttactc ataattcaat aatttaatat ttatagctac aattacaaac
     3301 tttgaactaa cactccgttt acaaactttt tatcatttaa ataaaagtta gctttttgat
     3361 ttatttttgg agaattatca ttttcacctt gaaaaattgc gcctttcgca tcatattcaa
     3421 aatgtccaaa ataattctct aattcgctta tttgaacaat gtaagcatta aaacaaatct
     3481 gaccgttaat taattcttct ttgtattgtg cagcaaaatt cgtgattatt tcaataccat
     3541 cataatttat tttgatgttt ttatataaaa aatcataaaa aactacttta ttttgaggaa
     3601 tactcaaata attatcaaat ctactatcaa ctatatcgtt ttcgcttaag atttcaaaac
     3661 tcgaaaccaa agccataaaa acatattttc gagtttcttc tctccaataa tcattctgaa
     3721 aatgcccagc ttcaaacaaa accgttggaa ctcccaaaaa ttgaaaagtg tctccgatac
     3781 aattaatatt aaatccatca tcaaaacgcc ctacttgacc cggaatataa ttttgcaaca
     3841 catcatttat tcccgcaatt aaattaatag cttttaatcg cgtttcattt acctctcttt
     3901 catcattata tgctggagcc aaaaaagaca aagttgccgg tttaccagta cttgcaaccc
     3961 caaaaatagt gcgctgatcg tgaagattaa aacaaaaatc aggtttaaaa cactcaaaag
     4021 cttctctcaa aaccctactt tcgggctgtg taagatcttg cgaatctcta ttcaaatcta
     4081 cttcgttggc attggcacga gtatataatt tcgctccatc tggatttagc atcggaatgg
     4141 cataaaaagt aaatgccttg agtaaggttt ttgctaattc tgaatctccg cttaacagat
     4201 ttaaaaaatc aaacaatgct tttgtcgtcg tactttcatt tccgtgcatt tgcgaccaaa
     4261 gcaaaatttt aatttttcct gtgccaattt gaaacttata aataggaata tctaaaaccg
     4321 attttccaat aatttctaat tgattattgg tgtttaattt ttctaatatt gggtaaattt
     4381 tgtctaaagt aatataacgc ccctcgattg attgttcttt atattgaccg aataattgtt
     4441 ctaaatccat aggttatttt ttgttttaca aaagtaaaca tctttacatt tacaaaagta
     4501 aacagcgaat ttaaaactaa atttataatt gtaaacagaa ataccctttc taatagaatc
     4561 tcttttaaca aagcaattga caattgtatt caattataat aatatacatt aaaatatttt
     4621 aaatcagtat tttaaattat tttaatttaa gttaaaattt caataacaac agcgatttta
     4681 aataatttac atctgtaatt ttgactgttc tatttttttt gattacattt gtaacacagt
     4741 tattataaaa cctattttac aatggtaaac accgatgatt ttataaaaag aatcgaaatt
     4801 atattagatt attacgggtt aaatgcttct tcatttgccg ataaaatagg tgtacaacgt
     4861 tctagcttgt cgcatttgct ttcgggaaga aacaaaccga gtttggattt tatacttaaa
     4921 attttggatg tttttccaga agttgatttg tattggatgc tcaatggaaa aggtaatttt
     4981 cccaaatcgg aattagcgat tgaatcgaat tctacttcac ctactcctat tttaaatact
     5041 caaaccgcag cttcagaaat tgtgaaacaa cccgatttgt tttcggaaaa attggtgctt
     5101 gaaaaaaatg aaaagttaga aaatcagttc cctcgttttt caaattcaaa aacagctata
     5161 attccaaaaa cagaaggtga aatagatcga attgtaattt tctataccaa cggaacattt
     5221 aaaacctatt cagccgaata aattattttt cttgtgaaaa tccattctct ggaatttttc
     5281 caacaacgcc attgaaatgc tgaagtaaaa taggcaaatc cttttcataa tttgaagtag
     5341 gaaaaaacgg ccgacccatt tttactgatt tttggccaaa atcaaaagca acagggatta
     5401 tgggaacatt tgcttttaag gcaatataat aaaatccggt tcttagttct tttacttttt
     5461 ttcgggttcc ttcgggagaa atggctaatc ggaattcgtc acgggtttca aaaattttgg
     5521 cgatcgcttc tactttgttt aaattcccta ctctatcaat gggcgctcca cccatccaat
     5581 ggaaataaaa accaaaagga aaacggaata attctttttt accaataaaa ttcatttgaa
     5641 ttccaatgat tcctcttgta aataatccga ggtaaaaatc gtgccaactc gtgtgaggaa
     5701 taaccatcag aacacatttt ttgatacttt catcaattgt gccttctatt ttccagccca
     5761 tccattggaa gaaaattaac ttgtagaaac tttttttcat ttagaaccca tttttcgtaa
     5821 aattaatatt tatcctgtat ttttgataaa aataaattca aatgctcaaa aaactgttta
     5881 gttacttact tccggtcacc attttcaaac aaaattcaac acttagtaaa actctagaag
     5941 taacttggac tgacggaaaa ttagttttag atagtgagaa tactaattat tcgtatggaa
     6001 atctacagcg aattttaaga aaagggttga aaatcattgg ttttgaaaaa atcaaatcaa
     6061 tgcaaaaaat cttagtgctt ggtgttgctg gtggcagcgt tatccgaact ttagttgacg
     6121 aaattaaatt tgaaggtcaa attactggtg ttgaaattga caaaaatata attgatattg
     6181 ccaataaata ttttcaactg aatgagattc caaatttgga aattattttt gatgacgctt
     6241 ccaaatttgt tttgaaaacg actgaaacct tcgacttgat tattatagat gtttttcaag
     6301 acacaaaaat gcccgatttt ttgtttgaat tctttttcat cgacagaatt tgctttttgt
     6361 taaatcccaa aggtcatatt atgtttaata ccatgttatt gaatcagcag cacagagaat
     6421 tcaatctaaa ctatgtgaaa aattttgatc ctaaaaaata taccgttacc ttatataaca
     6481 aaatggagaa aacgaatgaa ttgatactaa tcgaaagcaa ataatatttg taatttgtac
     6541 taaaaattaa gcaatttttt cgtaaattag agtccaattc gaatatctaa aagatgaaat
     6601 cgattctaaa aaaattgttc ttagggaatt tagccaaaat gccaaaaccc gaattcaatc
     6661 ctgttcagaa acgcttaatg aacataaaag ccatttggaa caataatcat caggacgata
     6721 atggaataga aaaatttgta cggttgttcc tttcttcttc gcaattgctt tttcctggag
     6781 tttatatcaa atatttggcc tatttaagag gctatgaata ccgagattta gccttagatt
     6841 tctatgtttt actcaaagtc agctttccat tattcatctt aatgaatcat tggcaaagtt
     6901 atcactttgt gatttggata atgatttatg tcttattaga aactatttta tacattccca
     6961 cgcttatttt tgcttcggat atgttttcga aaccgcgttc ttataaaaga tcgatgttat
     7021 tactttttct caattatatc gaaattatat tttcgtttgg agttttgtat tcttgcggtg
     7081 attatttaaa caagccgctc gatcattggt ttgatcctat ttatttcagc accatcaatt
     7141 cggcttctat cggctatggc gatttttatc ctgttacacc aatcggaaaa tttttaacta
     7201 ccgcccaagc ctttttgttc ttattatttg tagtcttatt tttgaatttt ttcactgcaa
     7261 aaataaaagt aaaaggctat tttgatattg aaaacgaaag ctaatttaaa tcaatcttct
     7321 agctttttct aaatcttctt tggtgtcgat tcctattcca atatgagttg tctcgaccat
     7381 tttgatgctt tttccaaatt ccagatagcg caattgttcc agtttttcgg aggcttccaa
     7441 tgatttcatc ggcagattgt agaaatccat taaagcttgt tttctgaatg cgtaaatccc
     7501 aatgtgtttc atatagcgaa cgcccacatt cgcttctcgc ggatacggaa tcaccgaacg
     7561 tgaaaagtat aaagcgaaac cattttggtt tacgaccact ttaacattgt tgggattatt
     7621 gatttcggct tcatccttga tttcactcat taaggaagcc aaatctactt tcttttcggt
     7681 gtcgttttta taaacttcaa tcagtttttc taaagcaacg gtgttaataa aaggttcgtc
     7741 accttgcaca ttcaccacaa tatccacatt gagattttcg acagcttcgg caattctgtc
     7801 gcttcctgat tcgtgttcct tgatgctctt tatggctttg cctccattgg aaacaatttc
     7861 gtcaaaaatt aaatcggaat ccgtcaccac aaaaacatcg tcaaaaagat gggattgttg
     7921 agctgcttcg taggttctca aaatcacggt ttttcctccc aaatcctgca tcaatttggc
     7981 gggaaatcgt gttgaggcat atcgtgcggg aatgactgct attattttca tttttatttg
     8041 tttctataat ctggagggtt gtttttgaat tcttcaatat attttttgaa ttctaaattg
     8101 ttttcaattt tataattccc taataatata cttttttgag gttcattttc gttgtataat
     8161 acaatatatt tatttccttt tttggggttt tcaggtagtt tataaaataa taaatcatag
     8221 ttatttatta tttttttatt gtttacaata tatgaatatg taatagaact ttttcctaca
     8281 ttaaatccaa tcgtacttct ttcaaagtaa acttcaattt caccaactga atatttataa
     8341 tttcctaaat attcattaag tctcccatta tcaattcgtt tggaataaaa aaacaatgct
     8401 aaactaacta aaaatataat gactataaat atgttcctct ttatcataat ttaattagta
     8461 atcactgtta gttaaatatc caaaattatt ttaatcctca aatataccat ttttccacaa
     8521 aacccatcta cactatcttc accgaagtca tactcaagga acccgcaact aatttatcat
     8581 tgaacaaact caattcttct gttttgtctt tcagtcccaa agtataaatc aagggcaaat
     8641 aatggtctgg cgttggaatt gcaatttgta atgccttgct ttgtttttcg taatcaataa
     8701 gtggttgaaa attgccgtcc aacaaataat tgtttactgt ttccctggct tcaattgccc
     8761 aatcgtaacc gtaattgtct ttgtcaaaat taggaaaatc gaccaatcgc aaattatgaa
     8821 cgatattgcc gctaccaatt atcaagattc cttttcggcg caaatcattc aatttcttgg
     8881 ccaattcaaa atgatattgc ggcgatttgg tgtaatcaat actcaactgg attacgggaa
     8941 cattggcttc
//
LOCUS       DAOSNN010000092         8856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7870_length_8856_cov_5.797978, whole genome shotgun sequence.
ACCESSION   DAOSNN010000092 DAOSNN010000000
VERSION     DAOSNN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7870_length_8856_cov_5.797978"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..617
                     /locus_tag="PLQ70_06150"
     CDS             108..617
                     /locus_tag="PLQ70_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HQE34234.1"
                     /translation="MEEIINKVANSALEVFDLEDYYPKGIRVQIDISQWFLEGFLLKE
                     KDFREQLKNHDWSQYQDQYVAINCSTDAIFPAWASILVAVQLAPFAKKIVNGTIEDLD
                     SSLYEEILSKIDYSVYDNKPVIVKGCSKKPVPMRAYILAANYLQPFARSIMYGEACSA
                     VPLYKSPRK"
     gene            689..1624
                     /locus_tag="PLQ70_06155"
     CDS             689..1624
                     /locus_tag="PLQ70_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HQE34235.1"
                     /translation="MIKKVLLLAVLLCISAKINAQTEAEKNLIKKTEEAAKVLDDTIK
                     NGWKTKGKITFLFNQANFNNWVAGGENSFSGNLGIDYKIDYKKDNFSWENRFLASYGI
                     LQTQNTDFEKKTDDQIEINSIVAKKTKGYWYYSFLVNFRTQFTKGYVYSKDSNGAEIR
                     TQNTNLMSPGYLLFGPGMYWKKTDNFKLNLAPLTAKFTFVDSFYTSIPGYVDNSYFGV
                     DANKSVLFQLGFNASAYYKFNIMENVTAENYLSLYSNYLEKPQNIDINYNLNIVMKIN
                     KVLSANLNFQAIYDDDAFAGFQTKEVFGVGVNYNF"
     gene            complement(1608..2666)
                     /locus_tag="PLQ70_06160"
     CDS             complement(1608..2666)
                     /locus_tag="PLQ70_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34236.1"
                     /translation="MKRYFLLFLLIFQMVFSQEIVVKGKAFNSGKFNDKVVYIIKNDT
                     IKKLRKHNDSLYENWKKNSKFQNRKDDSYREVSKNFGSDVLLFKNKNYCTHTDSLGNF
                     EIKAKLSDSLFFESHWHTTKKFLVADLIKKKKATIKLKLEPCEVWPSHPEKPTKLYVF
                     IGKKIKIWGSPSSYCNVGALSSRILSKYLVVKNIYGDFKKDTIQFTTYPPHSEVIHQN
                     YNPFRTSFSEYDYCLLYVAQYKDELLQATYIFDDVYLTKEGKWATPLKPKGLRNTIAP
                     DLFKPEKINFITPIEFEYEDRFYKEIYQIFPSAYNTVSDGKIIVNYGYYVEDLLEIRK
                     AGRLKEFDYLITGFKNYN"
     gene            complement(2773..3489)
                     /locus_tag="PLQ70_06165"
     CDS             complement(2773..3489)
                     /locus_tag="PLQ70_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQE34237.1"
                     /translation="MNTTPDLKVVYFPSCINRSMGKNSFQAEDDLQLTELTHQLLVRA
                     GFTIIYPEVLDSQCCGMPFSSKGFAEANHTQSEALENSLLKASENGKYPVLYDMSPCF
                     YHSKEEFSKALKIVDPIEFMLDYVMPNLTVKNKKETVAVFPVCSVKKIGKMNQLLALS
                     QLCAENVTMIDSNCCGFAGDRGFLIPELNEHGLRDLKAQIPANCNQGYSTSRTCEIGL
                     ESTSGIDFKSIFYLIEEVTR"
     gene            4088..5524
                     /locus_tag="PLQ70_06170"
     CDS             4088..5524
                     /locus_tag="PLQ70_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HQE34238.1"
                     /translation="MMKLIPKLNPNGWDEMFSSKGVRSSYRNVLHTLQNLSPENLEQK
                     QKQAADFFMNQGITFTVYSDDNQGIERIFPFDIIPRIITQKDWNEVELGIVQRLKALN
                     LFLEDVYNEQLIIKEGVIPASLISSCPHYIQEVHGIKLPHNIHIHIAGIDLIRGAKGE
                     FYVLEDNLRCPSGVSYMLENREITKRIFPEMLTSNHVSMVGNYPMILHNILVSMSPRH
                     ISNPNVVLLTPGVYNSAYYEHTFLARQMGVPLVEGRDLVVNNNNVYMKTTSGLQQVDV
                     IYRRLDDDYLDPLVFKPESALGVPGLISAYKMGNVALVNAVGNGVADDKAVYAYVPDM
                     IKYYLNEEPILKNVPTYQMENKEERELVFADMGNMVIKETNQSGGYGMIMGNKASDEE
                     LENGKKAILANPRNFIAQPIIQLSTVPCLIDGQLKLRHVDLRPYALFGPDGVKIVPGG
                     LTRVALTEGSLVVNSSQGGGSKDTWIIE"
     gene            5635..6588
                     /locus_tag="PLQ70_06175"
     CDS             5635..6588
                     /locus_tag="PLQ70_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HQE34239.1"
                     /translation="MKTNMLSRVADGMFWFNRYMERADGMVLTLHTFYILSFDKEMND
                     SQGYKPLLEYYTDLTKEQIIQSQYDSNFVLKYIICDNQNVNSVKNLVRKARENARGAQ
                     DKITKELWEHINSLYHFMNSPDLPRRLETYEALDIITSLKKDLLLYNGILHVTMPRGL
                     GWCFSSVGKNIERCLHTISLTQAYYKPINYNLEGEEDLLYWRRLLLSLSGYEQYLKSY
                     SNISHNRKVIQHVIFNKDFSHSVIYTLDLIDKYLVSLSIDNTSSEARTLQNQFGRLKS
                     LIKFTDYHHLNDEQLDEVLKDTKKLLNQFSNDFSKLFFSYT"
     gene            6594..7562
                     /locus_tag="PLQ70_06180"
     CDS             6594..7562
                     /locus_tag="PLQ70_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQE34240.1"
                     /translation="MAVFKIIHTTKYQYNWPIKESINEIRLFPHNFENQEVLQHQLLI
                     TSNPIVEVTKDFYGNNVGNFNILEPHKEMIIESRMIVKVNHSLKIPEIDTVKVQDLEV
                     EKEKNSYLLRLCYPENILQQKKITSYLKKINATEIPIIEIAQKCSEYIYKNYTYTKGI
                     TNIETTLDEILVHKKGVCQDFAHLLLQFLRTAGIPSRYVSGYVCPNSTGLRGEGATHA
                     WVEFYTPIQGWLGIDPTNNIWTMDNHVKLSVGRDFNDCSPVKGTFKGYAKQTLSVSVS
                     IGYEDGRQFEEVTDVKLKKLSEEVQRQLDLIEYQIMVQQRQQQQQQ"
     gene            complement(7634..>8856)
                     /locus_tag="PLQ70_06185"
     CDS             complement(7634..>8856)
                     /locus_tag="PLQ70_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQE34241.1"
                     /translation="IRDNEKLLQSVKKKEKSAVNVVIIQANKIKLKEKLINTTEKQAK
                     LLSNDIYINQLQINKLNRELTVLKQDYAEMIVKSYKSRSQQSRAMFVLSSNSFLQAYK
                     RAQYMKQYTGYRKMQGEEIKSKSNQLTIHNEKLGVQKSAKQKLIIENEKERLSLLKDK
                     QEQQKVVNSIKKDKNKIAAEIKKKQQEARTIDRQINRLIKEAIAEANRKAAMEKAMEK
                     AKAEAEARAKANNTKVDTKEEIKTRAKAIVSSSAPVSSSKIELTPEAKILSDNFKANK
                     GRLPWPVEKGVVSSTYGDHPHPVISSLMVHNSGVEITTDQGATARAVFAGEVTSVQIF
                     SPVNKSVVIQHGDYFTVYTNLREVYVRQGDKVNIKQNLGKIRTNGDSGKTVLKFSISQ
                     NTTYNNPASWLFNM"
BASE COUNT         2958 a         1431 c         1469 g         2998 t
ORIGIN      
        1 gaagccaaat tagaattggg aatgctttaa acggttattt tgtttgttgt ttatagttta
       61 ttgttctcaa ctacaaacta taaacaacaa accataaact tttcaaaatg gaagaaatta
      121 taaataaagt agccaatagc gccttggaag tttttgattt agaagactat tatccaaagg
      181 gaattcgtgt gcaaattgat atttcgcaat ggtttttgga aggttttttg ctgaaagaaa
      241 aagactttag agaacagcta aaaaatcacg attggtcgca ataccaagac caatatgtag
      301 ccatcaattg cagcaccgat gctatttttc cggcctgggc ttctatttta gttgccgttc
      361 aattggcacc ttttgccaag aaaattgtta atggtacgat tgaagattta gattcgtctt
      421 tatatgaaga aatcctttct aaaatagatt attcagtcta tgacaacaag cctgtgattg
      481 tcaaagggtg ttctaaaaaa cctgttccta tgcgtgctta cattttggca gcaaattatc
      541 tacagccatt cgctagaagc ataatgtatg gcgaagcttg ttcggccgtg cctttgtata
      601 aatcacccag aaaataaatc acttttttaa agaattgctt ttagtatttt atttatttta
      661 tatttgtagt ataactaata ctactcttat gataaaaaaa gtacttttat tagctgtttt
      721 gttatgtatt tcagctaaaa ttaacgctca aacagaggcc gaaaagaatt taatcaaaaa
      781 gacagaagag gcagccaaag tccttgatga caccataaaa aatggctgga aaactaaagg
      841 gaaaatcact tttcttttta atcaagccaa cttcaacaat tgggtagcag gaggagaaaa
      901 tagtttctcg ggaaacttgg gtatagacta caaaatcgac tataaaaaag ataattttag
      961 ttgggaaaat agattcttgg cttcttatgg aatacttcaa actcaaaata ctgattttga
     1021 aaagaaaacc gatgaccaaa tcgaaatcaa ttctattgta gctaaaaaga caaaggggta
     1081 ttggtattat tcatttttag tcaatttcag aacacaattc actaaaggat atgtgtattc
     1141 taaagacagc aatggtgcag aaattagaac ccaaaacact aacttaatgt cgccaggtta
     1201 tttgcttttt ggaccaggga tgtactggaa aaaaaccgac aatttcaaac ttaatttggc
     1261 accattaaca gccaaattta cttttgtaga tagtttctat acttcgattc cgggttatgt
     1321 tgacaatagt tattttggag ttgatgccaa taaaagtgta ttgtttcaat taggatttaa
     1381 tgcctcagct tactataaat tcaatattat ggagaatgta acggccgaaa attatttgag
     1441 tttatattct aattatttag aaaaaccaca aaacattgat atcaattaca acctaaacat
     1501 cgtaatgaaa atcaacaaag tattgtcggc taatttgaat ttccaagcca tttatgatga
     1561 tgatgctttt gctggatttc aaaccaaaga agtttttggg gtaggagtta attataattt
     1621 ttaaaaccag taatcaaata atcaaactct ttcaatcttc ctgcttttct gatttcaagt
     1681 aaatcttcaa cataatagcc ataattaact atgatttttc catcgcttac cgtgttgtaa
     1741 gctgatggaa aaatttgata tatttctttg taaaatctat cttcatactc aaactcaatt
     1801 ggagttatga aatttatttt ctcaggttta aataaatcag gagctattgt gttgcgtaat
     1861 ccttttggtt tcaaaggagt tgcccatttg ccttcttttg tcagatatac atcatcaaaa
     1921 atatatgttg cttgaagtaa ttcgtctttg tattgcgcta catacaataa gcaatagtca
     1981 tattccgaaa agcttgtcct gaatggattg taattttggt ggattacttc tgaatgtggt
     2041 ggataagttg taaattgaat agtgtctttt ttaaaatcgc cataaatatt tttaactact
     2101 aaatattttg acaaaattct agaacttaaa gcgcccacat tgcaataact tgaaggagat
     2161 ccccaaattt taattttctt gccaatgaaa acgtatagtt ttgttggttt ttcaggatgt
     2221 gaaggccaaa cttcacaagg ttctaatttt aacttaatag ttgctttttt cttctttatt
     2281 aaatcagcaa ccaagaattt tttagttgta tgccaatgag attcaaaaaa aagagaatca
     2341 gataatttag cttttatttc aaaattaccc aagctatccg tgtgagtgca ataattttta
     2401 tttttaaaca atagtacatc acttccaaaa tttttagaaa cctctcgata ggaatcgtct
     2461 tttctgtttt gaaattttga attctttttc caattttcat ataaagaatc attatgtttt
     2521 cttagttttt taattgtgtc attttttata atataaacaa ctttgtcatt gaattttcct
     2581 gaattaaagg cttttccttt tactactatt tcttgtgaaa acaccatttg aaatattagt
     2641 aaaaacaata aaaaatatct tttcatttta gattatttag agcttacaat atacggtttt
     2701 tctttcgaaa ttgtatttaa aaatgatctt ctgtgttagg agaaagtaat atctagggat
     2761 tagaaaaata tattacctcg tcacttcctc aatcaaataa aagattgatt taaaatcaat
     2821 tccgcttgtg ctttccagtc caatttcgca agttctgctg gtggaataac cttgattgca
     2881 attggcggga atttgggctt tcaaatcacg caatccgtgt tcgttgagtt caggaatcag
     2941 gaagcctcta tcgccggcaa aaccacaaca attactgtca atcatagtaa cattttctgc
     3001 gcaaagttgt gataaggcaa gtaactgatt catttttcca atttttttaa ccgaacaaac
     3061 tggaaaaacc gcaacagttt cttttttgtt ttttaccgtt aaatttggca ttacataatc
     3121 cagcataaat tcgatgggat cgacaatttt taaggctttc gaaaattctt ctttcgaatg
     3181 gtaaaaacac ggactcatat catataaaac aggatatttt ccgttttcag aggcttttaa
     3241 aagtgaattt tctaatgctt cagattgggt atgattggct tcggcaaaac ctttgcttga
     3301 aaaaggcatt ccgcaacatt ggctatccaa aacttcggga taaatgatgg taaatccggc
     3361 acgaaccaat aattgatgcg ttagttcggt caattgcagg tcgtcttcgg cttgaaaact
     3421 atttttgccc atacttctgt tgatacagga agggaagtac actactttta aatcaggggt
     3481 tgtgttcatt tttttctcgt tgggtttagg cttcgaatta gtgcatcaat cgtagcaact
     3541 aaatcatttt acttttcgtg tattaaagct tgaaaaactg gatttacaaa acggatagtt
     3601 atattaattt ttgcgtttaa aaagggaagt tactaaattc aacctaagaa cacaatagca
     3661 tttataatta tgaagtcttt gttttacaac tacaaattta aaaaagagat aaagacattc
     3721 tgtagttatt tagaattaat ttaatatttg actcttttta tattgaagtt aaatttacat
     3781 taagtttaca ttacccctat atttttacat tagtatgcac tgaaatagtg tctatttaac
     3841 aatcataccc tataataaaa ggggtaaaat tcaattttta ttgtcttttg gtttaacgaa
     3901 ttaaaaaatt gaagctaaaa aataataaat ttgtcaaaat agaaagttaa aaagaagaat
     3961 atcacataaa ttattaatta ttcatcataa taaacatttt gattaaaata aaacaataaa
     4021 tttgtctaaa actatttatt ttataaataa caacctgaaa agtttgacta tttaacaatg
     4081 caatactatg atgaagttga tacccaaatt gaaccctaat ggatgggatg agatgttttc
     4141 gagtaaagga gtgagaagtt cctatcgcaa tgttttgcat accttgcaaa atctaagccc
     4201 agaaaactta gaacaaaaac aaaaacaagc agcagatttc tttatgaatc aagggattac
     4261 gttcactgta tatagcgacg ataatcaagg tatagaaaga atatttccgt ttgatatcat
     4321 ccccagaata attactcaaa aggattggaa cgaagtagaa ttgggtattg tacagcgatt
     4381 aaaagcctta aatctgtttt tagaagatgt ttacaacgaa caacttatca taaaagaagg
     4441 tgttattccc gcctcattga tttcgtcatg tcctcattat attcaggaag ttcatggtat
     4501 aaaattaccc cataatattc atattcacat tgcgggaatc gatttgattc gaggtgcaaa
     4561 aggagaattc tatgtactag aagacaatct tagatgtccg agtggtgtga gttatatgct
     4621 tgaaaacaga gaaatcacca aacgtatttt tcctgaaatg cttacttcaa atcacgtgag
     4681 catggtagga aactatccga tgattctgca caacatactg gtttcgatgt cgccaagaca
     4741 catttcaaat ccaaatgtag ttttactaac tcctggggta tataattctg cttattatga
     4801 gcatactttt ttggccagac aaatgggagt tccattggta gaaggaagag atttggttgt
     4861 gaacaacaac aatgtgtata tgaaaaccac ttctggcctt caacaagtgg atgtgattta
     4921 cagacgattg gatgacgatt atcttgatcc cttagtattt aaacctgaaa gtgcattagg
     4981 tgttccagga ctgattagcg cctataaaat ggggaatgta gccttagtaa atgctgttgg
     5041 aaatggagtt gccgatgaca aagctgttta tgcttatgtt ccggatatga ttaaatacta
     5101 tcttaacgaa gagcctattt tgaaaaatgt tccaacctat caaatggaaa acaaagagga
     5161 acgcgaacta gtctttgccg atatgggaaa tatggtaatc aaagaaacca atcagagtgg
     5221 tggttatgga atgattatgg ggaataaagc tagtgatgaa gaacttgaaa acggaaaaaa
     5281 agcgattctt gccaatccta gaaatttcat tgcacaacct attattcagc ttagtactgt
     5341 gccgtgtctt attgatggtc agctaaagtt gagacatgtt gatttaagac cttatgcatt
     5401 attcggtcca gatggagtta aaattgttcc aggaggactt actcgtgtag cactaacaga
     5461 aggctcattg gtagtaaatt cttcgcaagg tggcggtagt aaagacactt ggattataga
     5521 ataaaaagtt tttttaataa acactatcaa aatgaaacgc ccaagaatac tttttcagaa
     5581 ttgaaaaaaa atttcaagcg gtttcattat acgatacata aataaaacat acagatgaaa
     5641 acaaatatgc taagtcgagt tgccgacgga atgttttggt tcaacagata tatggaaaga
     5701 gctgatggaa tggtactaac gcttcacact ttctatatct tgtcatttga caaagaaatg
     5761 aatgattctc aaggatacaa acctttattg gaatactata ccgatttgac aaaagaacaa
     5821 ataattcaat cgcaatatga ttcaaatttt gtgttgaaat acattatttg cgacaatcaa
     5881 aatgtgaatt cggttaaaaa cctagttaga aaagcccgcg aaaatgctag aggagctcaa
     5941 gataaaataa ctaaagaact ttgggaacac atcaactctt tgtatcattt tatgaattcg
     6001 cccgatttgc caagaagatt agaaacctat gaagctcttg atattattac aagtctcaaa
     6061 aaagatcttt tgctctacaa cggaattctt cacgtaacaa tgccacgagg tttgggctgg
     6121 tgtttttcta gcgttggaaa aaacattgaa cgttgcttac acaccatttc cttaactcaa
     6181 gcttattaca aacccattaa ttacaacctt gaaggagaag aagatttatt gtactggaga
     6241 cgtttgcttt tatctctttc aggatacgaa caatatctaa aaagctatag caacatttct
     6301 cacaatagaa aagtaataca acatgtgata tttaataaag atttttcaca ttcggttatt
     6361 tatacgttag atttaattga caaatattta gtttccttat ctattgacaa tacttcaagc
     6421 gaagccagaa ctcttcaaaa ccaatttgga agactaaaaa gtttaatcaa atttaccgac
     6481 tatcaccact tgaacgacga acaattagac gaagttttga aggatactaa aaaactattg
     6541 aatcagtttt cgaatgattt ttctaaatta tttttctcct atacctaaaa aaaatggctg
     6601 tattcaaaat aattcatacc acaaaatacc aatacaattg gccgataaaa gagagcatca
     6661 acgaaattcg tttgtttcct cacaattttg aaaaccaaga ggtattgcaa catcagcttc
     6721 taattacaag caatcctatc gttgaagtca caaaagattt ttatggtaat aatgttggta
     6781 attttaacat tttggaacca cacaaagaga tgattatcga atctcgaatg atagtaaaag
     6841 tcaatcattc gctcaaaata cccgaaattg acacggttaa agttcaggat ttagaggtag
     6901 agaaagaaaa gaacagttat ttgcttcggc tttgttatcc agaaaacatt ttgcaacaaa
     6961 agaaaatcac ctcttatcta aaaaaaataa atgccaccga aatacctatt atagaaattg
     7021 ctcaaaaatg tagcgaatac atctataaaa actataccta taccaaagga attaccaata
     7081 tcgaaacgac tctagacgaa atactagtgc ataaaaaagg cgtttgtcaa gattttgcac
     7141 acctattact acagtttttg cgcactgctg gaattccttc aagatatgtg agtgggtatg
     7201 tttgtccaaa ctctacaggt cttagaggag aaggagcaac tcatgcttgg gtcgaatttt
     7261 atactccaat acaaggctgg ctcggaattg atccaactaa taatatatgg acgatggaca
     7321 atcacgttaa actttcagtt ggtcgtgatt ttaatgattg tagtcccgta aaaggaacat
     7381 tcaaaggata tgcaaaacaa accctttcgg ttagcgtttc gataggatat gaagatggaa
     7441 gacaatttga agaagtaacc gatgtaaaac tcaagaaact ttcggaagaa gtacaaagac
     7501 aacttgatct catagagtat caaattatgg tgcaacaacg ccaacagcag cagcaacaat
     7561 aattttaaat ataacaaagc gccattttaa tttcgattaa aatggcactt ttttttatgt
     7621 ctaaatctat gtattacata ttaaacaacc aactggctgg attgttatag gttgtgtttt
     7681 gtgaaataga aaactttaga accgtttttc cgctatctcc attggttctt atttttccta
     7741 aattttgctt aatattaact ttatctccct gtctcacata gacttctctt aggtttgtat
     7801 aaacggtgaa ataatcccca tgttgtatca caaccgattt atttacaggt gaaaaaatct
     7861 gaacactggt tacctctccc gcaaatacgg ctcttgctgt tgctccttgg tcggttgtga
     7921 tttcgacacc actattgtga accattaatg aactgattac tggatgtggg tgatctccat
     7981 aagtcgaaga aacaactcct ttttcgactg gccaaggcaa tcttcctttg ttggctttga
     8041 aattatccga cagtattttg gcttctggag tcaattctat tttggatgaa gaaacaggcg
     8101 cagatgagga aacaattgct tttgcacgtg tttttatttc ttctttggta tctactttag
     8161 tgttattagc tttggctctg gcctcagctt cggctttagc tttttccatc gctttttcca
     8221 tcgctgcttt tctattagct tcggctatgg cttcctttat caaacgatta atttgtcgat
     8281 cgattgttct tgcttcctgt tgttttttct tgatttcagc agcaatttta tttttgtctt
     8341 ttttaataga attaaccact ttttgttgct cttgcttgtc ttttagtaac gacaatcttt
     8401 ctttttcgtt ttctataatc agtttttgct tggccgattt ttgaactcct aatttttcgt
     8461 tatgaatcgt taattgattt gattttgatt taatttcttc tccctgcatt ttcctataac
     8521 cggtatattg tttcatatac tgggctcttt tgtaagcttg cagaaaacta ttcgatgaca
     8581 aaacaaacat cgctctgctt tgttgcgatc tacttttata ggactttaca atcatctcag
     8641 cataatcttg cttcaaaaca gtaagttccc gattcagttt gtttatttga agttgattaa
     8701 tgtaaatatc gttactaagc aactttgcct gcttttcggt agtattaatc agtttttctt
     8761 tcagctttat tttattggct tggataataa caacatttac agctgatttt tcttttttct
     8821 ttaccgattg caacaacttt tcattatctc taattt
//
LOCUS       DAOSNN010000093         8846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_7880_length_8846_cov_4.543852, whole genome shotgun sequence.
ACCESSION   DAOSNN010000093 DAOSNN010000000
VERSION     DAOSNN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7880_length_8846_cov_4.543852"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PLQ70_06190"
     CDS             <1..808
                     /locus_tag="PLQ70_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34242.1"
                     /translation="SLTNPKIEYFSAIDTERRMYNSLEVKGIPHCILIDPNGIVRWEG
                     YPILTGYELTSEVIEDIIEKYKGNTQNDQISDYSNYTLKELSNLKTVKELEQNYVLVN
                     HYLEQIERGDIDHMALTKIDIGLVNPYWENVPELKIYNDNWKKASSEFGDFIKKNAPE
                     KKELLDQYQKKTIDKEEYFRKHREIMTRLQADYPNEFPQLSEKQINSLRTMWKQTGRY
                     MLEDYKKKEKIFPTYWIPEKDLENSKKTKEYKTINQELLLVNNEIIKKKI"
     gene            814..1383
                     /locus_tag="PLQ70_06195"
     CDS             814..1383
                     /locus_tag="PLQ70_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34243.1"
                     /translation="MSTSILNSILRKSKENKEIISIWLYNTDKGSFVGYITEINEEYI
                     GFRHFTRFGKQDGVIFIKVANIKNIDFNDDYTKVMECLIEYSDIIDKPSEFSISLNQS
                     ENWQFDALLQLHKAKEQMASFEINGNEYFTGFVKDISDEDFIVNCVAKNGEDLGTSLF
                     KIEDVTEIRVNDIDDRRRLLLYKWRKASL"
     gene            1772..2902
                     /locus_tag="PLQ70_06200"
     CDS             1772..2902
                     /locus_tag="PLQ70_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HQE34244.1"
                     /translation="MIEKDKRIQIFDIAKGISIILMTVGRYDFTDIYPNLLKFQNVAV
                     FLRMPTFIFISGYLFSDRLNFKEFLCHKIDGLLKPLLGFILSLTLLNIIFDAVALNIF
                     TFKSVFVHITNLARYFYHGSFDVINVSFWFIGALFLGQVALKGFLEIKGFKKPLNYFL
                     LTAFFLVLFFINTIKFKFYWSEYIPIFFTYLLIGHGFKKFSTRFLDGTSFFYSKIIIV
                     FPVLFFMSIFLLNQLDIKMNFNLAGLEFNYHYLLGLSLLGVFTVLYFCRYIEKIPFLN
                     SFLVYCSKASFFILAYHIFIKNVYKVVFDMKSYHPVLHTLLFILNIILCCLIYRFLQK
                     VPFVRLLFYPIKVISLNNAEINLLKSKYINKFIPQDVSLGIA"
     gene            2937..3374
                     /locus_tag="PLQ70_06205"
     CDS             2937..3374
                     /locus_tag="PLQ70_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQE34245.1"
                     /translation="MALINPHINFNGNAEEAFTFYKSVFGGEFAMIMRFKDLENPEFP
                     VAESEAHKIMHIALPIGQNILMGNDVPEIMGRVNENENRSKIAISAESREEADRLFTG
                     LSAGGTIEMPIEDSPWGSYFGMFRDQYGIEWMVDFDPKYKGKI"
     gene            complement(3489..4769)
                     /locus_tag="PLQ70_06210"
     CDS             complement(3489..4769)
                     /locus_tag="PLQ70_06210"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQE34246.1"
                     /translation="MQIVIIGGGFAGINLAKDLSNHKGIEVILVDKNNYNFFPPLIYQ
                     VATAFLEPSSISYPFRKFFAGKKNLQFRLGELQKVIPAENKIILNNGELHYDQLVFAT
                     GAETSFFGMENVKKNAIPMKTLNDAIEMRNTLLKNLEKAAITKDIRKRRQLLTIVVAG
                     GGPTGVEVSGMFAEMRKNILLKEYPELETSASNIYLVDGGDALLSPMSKESQADTLEA
                     VTKLGVLVKLNTRVVDYKDDTVYFENGETIQTKNLIWAAGVSAKVFEGIPTESYGRGK
                     RMITDAFNKVNTTENIYAIGDTCIQLSDENFPSGHPQVAQVAIQQGENLAENFKMMLK
                     NKPLKPFKYNDKGSMAIIGKNKAVVDLPKPKMHFKGFLAWMIWLFVHLMSLITYRNRI
                     NTFYHWMIAYFSKDQSLRMIIRPDKKSKVEAKPE"
     gene            complement(4852..6189)
                     /locus_tag="PLQ70_06215"
     CDS             complement(4852..6189)
                     /locus_tag="PLQ70_06215"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQE34247.1"
                     /translation="MSKQFSDLGIQESIIKALDDLKIVVPTEIQQKTISLLLTTQTDL
                     VGLAKTGTGKTAAFGLPLLQLIDSKITDVQAVILAPTRELGHQIFSNLEAFSKYIPEI
                     SIAETCGGIPIKPQIERLTLPTHIVVATPGRLIDLIQRKAISLKETKFLVLDEADEMV
                     AILKEGLDEIIAELPKNRRTFLFSATMPGTIKQLIQNYLNKNVVQVSADMQTLGNQGI
                     DHQYIVVDPIEKLDVLMHFLTSKEGERGIIFCKTKAAVNKLAKNLAINRFSSGALHGS
                     LSQGIRDRIMEQFREGHIHILVATDLAARGIDVKEMAYVVNYHLPDTYENYVHRSGRT
                     ARAGAKGLSLTVLQQEEVAEIADFERELGIKFTKFQKPTAENIEENNTLLWAKQIFKT
                     KPNHEVSAEFKNKVKTIFHHLTKDELIEKLLANQLLQSKADSNKEKPVKKFKN"
     gene            complement(6320..7858)
                     /locus_tag="PLQ70_06220"
     CDS             complement(6320..7858)
                     /locus_tag="PLQ70_06220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQE34248.1"
                     /translation="MSQNTLETQIEDKKELYSYQKGDIESIFERLDNASPNYHLLYQL
                     PTGGGKTVVFSEIVRQYLSKHNKKVVVLTHRIELCKQTSKMLKGFDVKNKIINSKVKE
                     LPDQNDYSCFVAMVETLKNRINDEKLHLDNIGLVIIDEAHYNSFRKLLSSFKNAFILG
                     VTATPLSSNIKLPMNESYDELIVGDNISSLIEKGFLAKAITYSYDVGLTSLKVGINGD
                     YTVKSSDDLYTNMAMQEKLLHAYTEKSLGKKTLIFNNGINTSLYVYETFREAGYGIRH
                     LDNTSSTEERKEILHWFKHTPDAILTSVGILTTGFDEPTVETIILNRATKSLTLYFQM
                     IGRGSRKLPGKEEFTVIDLGNNAARFGLWNEPVNWQHIFKSPEFYLENLRDDTEIELY
                     FKYTMPPELRAKFSKTEVVTFDVDEEHKLAIAQNLRSKVVLDKSLEQHAAMCVDNTEE
                     LRDAKELYKELDPDIECRLKRYSKCLSQCSKNYREWLVDDYKQKLLLLIGKKYREKIM
                     NEAD"
     gene            complement(7967..8497)
                     /locus_tag="PLQ70_06225"
     CDS             complement(7967..8497)
                     /locus_tag="PLQ70_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6155 family protein"
                     /protein_id="HQE34249.1"
                     /translation="MSKRDLKKYLNELTKSQLEEQIIELYEKFVPVKNYYDFVFNPKE
                     DTLLRESKIKISNEYFPARAASGKARKPKMRRSVAQKYIKQFMLLGVDPFIIADVMLY
                     SIEIAQTFSSEKIIKSELFYKSMLNSFSQAVSFMIANGILSEFKTRIIAINKETFNQK
                     WFNFQEFDAILERFEY"
     gene            8601..>8846
                     /locus_tag="PLQ70_06230"
     CDS             8601..>8846
                     /locus_tag="PLQ70_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HQE34250.1"
                     /translation="MLKVFKNIAILEGISYLVLFFNMLYIKHSNIELYHSLLNPLGMT
                     HGLLFIGYVLLAVWLKKIQEWNNQTLIIILGASLLPFA"
BASE COUNT         2818 a         1476 c         1527 g         3025 t
ORIGIN      
        1 aagccttacg aatcctaaaa tagaatattt cagtgccatc gacaccgaaa gaagaatgta
       61 taattcgctg gaagtgaagg gaattccaca ctgtattttg atagatccta atggcatcgt
      121 acgttgggaa ggttatccca tattaacggg ttatgagtta acatcggaag taattgaaga
      181 cattatcgaa aagtacaaag gcaacactca gaatgaccag atttcggatt attccaacta
      241 tactttgaag gaattatcca atctaaaaac ggtcaaggaa ctagagcaaa attatgtttt
      301 ggtcaatcat tatttagagc aaatcgaaag aggcgacatt gaccatatgg cattaactaa
      361 aatcgatata ggtttagtaa atccttactg ggagaatgtt ccagaattga aaatctacaa
      421 cgacaattgg aaaaaagcat catcagagtt tggtgatttt atcaaaaaga acgctcctga
      481 aaagaaagaa ttactcgatc aataccaaaa gaaaactatc gataaagaag aatattttag
      541 aaaacatcgt gaaataatga cacgattgca agccgattat cctaatgaat ttccacaact
      601 ttcagaaaag caaatcaata gtcttagaac aatgtggaaa caaaccggaa gatatatgtt
      661 ggaagattat aaaaagaaag agaaaatttt tccaacctat tggattccag aaaaagattt
      721 agaaaactca aaaaaaacaa aggaatacaa aacgattaat caagaacttt tgcttgtaaa
      781 taatgaaata attaagaaaa aaatataatc tgtatgagca ccagtatttt aaattcgata
      841 ttgcgaaaat caaaagaaaa caaagaaatc atttcgatat ggttatacaa taccgacaaa
      901 ggttcatttg taggttacat caccgaaata aatgaagaat atatcggttt tagacatttt
      961 acacgatttg gaaaacaaga tggcgtaatt tttataaaag tcgccaacat taagaacatc
     1021 gatttcaatg acgattacac caaagtgatg gaatgcctca tcgaatattc cgacattata
     1081 gacaaacctt cggaatttag tatttcgtta aatcagtctg aaaattggca atttgatgcg
     1141 cttttgcaac ttcataaagc caaagaacaa atggctagtt ttgaaatcaa cggaaatgaa
     1201 tactttacag gatttgtcaa agatatttcg gatgaagatt ttattgtgaa ttgtgtggcc
     1261 aaaaatggag aggatttagg aacttcactt ttcaaaatcg aagatgttac tgaaatcaga
     1321 gtcaatgata ttgatgatag aaggagatta ttattgtata aatggagaaa agctagtttg
     1381 taaaatagtt ttttaagatg ccatccatcc cttctcttca aagttgagca aacaaaaaca
     1441 aacctactat agatttatac aattgtcact ctggaagcgt ctcaacacac gagagaactt
     1501 gctctagacg cttccagcgt gacaaataat gttgattcca actcatccca aaaaccagtt
     1561 caaaaaaaat caaaaacaaa attatttatt caaaaacgtt ttgtagatta gcaaaatcaa
     1621 taatcatcag atttaattca ttattataca atgaattact gtttagcccc aaatatacta
     1681 ttgaaccaaa agaaagaatg attttagata ggtttgtcta tcaaaatcaa ataataacgt
     1741 aagttaagaa atcaatctaa aggtcaagat tatgatagaa aaggacaaaa gaattcagat
     1801 ttttgatatt gctaaaggaa ttagtattat tttgatgaca gtggggcgct atgactttac
     1861 ggatatttat cccaatttgt tgaagttcca aaatgtagca gtgtttttga gaatgcccac
     1921 ttttattttt atttcaggtt atctctttag cgatcggtta aatttcaagg agtttttatg
     1981 ccataaaata gatggtttac tcaaaccttt attgggtttt atacttagtt taactttatt
     2041 gaatataatt tttgatgcag ttgctttaaa tatatttact ttcaaaagtg tttttgtaca
     2101 catcacaaat ttggctcgat acttttacca cggcagtttt gatgtaataa atgtttcgtt
     2161 ctggtttatt ggagcgctat ttttaggtca ggttgcacta aaaggttttt tggagattaa
     2221 aggatttaaa aaacctttaa attatttcct gttgacggct ttttttctgg ttctattctt
     2281 catcaataca attaaattca aattttattg gtctgaatat attcctattt tctttaccta
     2341 tttattgatt ggccacggtt ttaaaaaatt cagtactcgt tttcttgatg ggacttcctt
     2401 tttttacagt aagataataa ttgttttccc tgtattattt tttatgtcca tctttctatt
     2461 gaatcaattg gatattaaaa tgaattttaa cttagctgga ctcgaattca attatcatta
     2521 tcttttggga ttatctcttc ttggggtttt tacagtgctt tatttctgtc gttatattga
     2581 aaaaataccg tttctaaatt catttttggt ctattgttct aaagcgagtt tttttatttt
     2641 ggcctatcat atttttataa aaaatgttta taaagttgtc tttgacatga aatcttatca
     2701 tccggtattg catactttgt tgtttatttt aaacatcata ttgtgttgcc taatttatag
     2761 attcttgcaa aaagtgccat ttgttcgcct tttattttat cctatcaaag taatttcgtt
     2821 gaataatgct gaaatcaacc tattgaaatc caagtatatt aataaattta ttccacaaga
     2881 tgtttctctt ggaattgctt aaagagaata gcaaacaact taacaactaa aaaattatgg
     2941 cacttatcaa tccacacatt aacttcaacg gaaatgccga agaagcattc actttttaca
     3001 aatcagtatt tggcggagag tttgccatga ttatgcggtt caaagattta gaaaaccccg
     3061 aatttccagt ggctgaaagt gaagcacata aaataatgca cattgcttta cccattggtc
     3121 aaaatatttt gatgggcaat gatgttcccg aaataatggg acgagtaaat gaaaatgaaa
     3181 acaggtctaa aatagccatt agtgcagaaa gtcgtgaaga agccgacagg ttgtttaccg
     3241 gactttcggc aggcggaact attgaaatgc ccattgaaga cagcccttgg ggttcctatt
     3301 tcgggatgtt tagagaccaa tatggcatcg aatggatggt agattttgat ccaaaataca
     3361 agggtaaaat ctaagaaaca aacaaagaaa tacctaataa taaggatttc gttgtttgtt
     3421 caacgtcagc atttaggaga atttcaaaat ctttattcta atttaaaacc ccgatttcta
     3481 aatcgaaact attcgggttt tgcttcaact tttgattttt tgtcgggtct gataatcatt
     3541 cgcaaagatt ggtctttcga gaaataggca atcatccaat ggtaaaaagt attgatacga
     3601 ttgcgatagg taatcaaaga catcaagtga acaaacaacc aaatcatcca agccaaaaag
     3661 cctttgaagt gcattttagg tttgggcaaa tctacaaccg ctttgttttt tccgatgatc
     3721 gccatagaac ctttgtcgtt gtatttaaag ggtttcaacg gcttgttttt tagcatcatt
     3781 ttgaaatttt cggctagatt ttcaccttgc tgaatggcaa cttgagccac ttgtggatga
     3841 cctgatggga aattttcatc actaagttga atacatgtat ctccaatggc gtagatgttt
     3901 tcggtagtat tgactttatt gaaagcatcg gttatcattc tttttccacg accataactt
     3961 tctgttggga ttccttcaaa aactttggca gaaactccag ctgcccagat taaatttttg
     4021 gtttgtatcg tttctccatt ttcaaaataa acggtatcgt ctttataatc gactactcgg
     4081 gtatttagtt ttaccaaaac gcccagcttt gttactgctt caagtgtatc tgcttgcgat
     4141 tctttgctca tgggcgacaa gagtgcatcg ccaccatcga ccaaataaat attactggca
     4201 gaagtttcta attcgggata ttctttgagc aaaatgtttt ttcgcatctc ggcaaacatt
     4261 cccgaaactt ctactcctgt tgggccacca ccagcaacta caatggttaa gagctgtctt
     4321 cgtttgcgaa tgtctttagt tattgctgct ttttctagat ttttcaacaa tgtgtttcgc
     4381 atttcgatgg catcattgag cgttttcatc gggatggcat ttttcttgac attctccatt
     4441 ccgaaaaagc tggtttcggc tccagtggca aagaccaatt ggtcgtaatg taattcgccg
     4501 ttgttgagaa tgattttgtt ttcggcagga attacttttt gcaattcgcc caaacgaaat
     4561 tgaaggttct ttttgcccgc aaaaaatttt ctaaagggat aactaatact ggaaggttcc
     4621 aaaaaagccg tagctacttg ataaataagg ggcggaaaga aattatagtt gtttttatcg
     4681 acaagaatta cttcaatgcc tttatgattt gataaatctt tagccaagtt aatcccagca
     4741 aaacctcctc ctattattac tatttgcata ttgaaaacga atttagtgat agcaattact
     4801 cattagataa ttaatatcgc taaaagtaca atataatttt tggcgcaaat attagttttt
     4861 aaatttttta acaggttttt ctttgttgga atcggctttg ctttgcagca attgattggc
     4921 caataatttt tcgattaatt catctttggt caaatgatga aaaatggttt tgactttgtt
     4981 tttaaattcg gccgaaactt cgtgattggg tttagttttg aagatttgtt tggcccacaa
     5041 aagcgtgttg ttttcttcaa tattctcggc agttggtttt tgaaatttgg taaatttaat
     5101 tcccaattct ctttcaaaat cagctatttc agccacttct tcttgttgca aaaccgttaa
     5161 cgaaagtccc tttgctcctg ctcttgcggt tcttccgctg cggtgaacat agttttcata
     5221 ggtatctggc aaatgataat tgaccacata tgccatttct tttacgtcaa ttcctctggc
     5281 ggccaaatcg gttgctacca aaatatgaat gtgtccttca cgaaattgtt ccataattcg
     5341 atcccgaatg ccttgtgaca aactgccgtg caaagctcct gacgagaatc ggttaatagc
     5401 caagtttttg gctaatttat tgacggcggc tttggttttg caaaaaataa tgccccgttc
     5461 gccctctttt gaagttagaa agtgcatcaa aacatccaat ttttcgatgg gatcaacaac
     5521 gatgtattga tggtcaattc cttgattgcc tagagtttgc atatcggcac tcacttgaac
     5581 tacattttta ttcaaatagt tctgaatcaa ttgtttgata gttccaggca ttgttgccga
     5641 gaaaagaaat gttctacggt ttttgggtaa ttcggcaatg atttcatcta aaccttcttt
     5701 caaaattgca accatttcgt cggcttcatc gagtaccaaa aatttagttt ctttcaaact
     5761 gatggctttg cgctgtatca aatcaatcaa tcttccgggt gttgccacta cgatatgggt
     5821 tggcaaagtc aatcgttcga tttgcggttt tattggaatt ccgccgcaag tttcagcaat
     5881 agaaatctcg ggaatgtact tggaaaaagc ttctaaatta ctaaaaattt gatgtcccaa
     5941 ttctcttgtt ggtgctaaaa taacagcctg aacatcagta attttagaat caattaattg
     6001 aagtaatggt aagccaaaag cagctgtttt tccagttcct gttttggcca atccaacaag
     6061 gtcggtttgg gttgtcaaaa gcaaggaaat tgttttttgt tggatttcgg ttggcacaac
     6121 aatttttaaa tcatcaagtg cttttataat agattcttga atacctaaat cagagaattg
     6181 ttttgacata ttgttgattt attatttatt aaattgttgt tttgaaacgg attcctagcc
     6241 ccgatagtag tgaaaatcct attgtgccgg tgttcgacac aatagattgt aacgaatggc
     6301 gggaaatagc tcctgaaaat taatcggctt cgttcataat tttttcgcga tactttttcc
     6361 ctatcaataa cagcaatttc tgtttgtaat catctaccaa ccattcgcgg taattcttgc
     6421 tgcattggct caaacatttg gaataacgct tcaaacggca ttcgatatcg ggatccaatt
     6481 ctttatacaa ttctttggca tcacgcaatt cttctgtatt gtccacacac attgctgcat
     6541 gttgttccaa agatttgtcc aagacaacct tggaacgcaa attttgggca atggctagtt
     6601 tatgttcttc atcgacatca aaagttacga cttcagtttt actgaattta gcacgtaact
     6661 ctggaggcat cgtatattta aaatataatt caatttcggt atcgtcacgt agattttcaa
     6721 gataaaactc tggcgatttg aaaatgtgtt gccaatttac gggctcattc cacaagccaa
     6781 aacgagcggc attgtttcct agatcgataa ctgtaaattc ctcttttcca ggcaattttc
     6841 gagaaccacg accaatcatt tgaaaataca aagtcaatga ttttgtggca cgattcaaaa
     6901 taatagtttc tacagtaggt tcatcaaaac cagtggtaag gattccaacc gaggttaaaa
     6961 tagcatcggg agtgtgtttg aaccaatgca aaatctcttt acgttcttcg gtactactag
     7021 tattatcgag atgtcgaatg ccgtatcctg cttcacggaa agtttcatat acgtataaag
     7081 atgtattgat tccgttattg aaaatcaggg tttttttacc caaagatttc tcggtgtaag
     7141 cgtgcagtaa tttctcttgc atcgccatat tggtatataa atcatcagat gatttcacgg
     7201 tataatctcc attgattcca actttcaatg aggttagacc aacatcataa ctataagtaa
     7261 ttgctttggc taagaagcct ttttcaatta acgaactaat gttgtctcca acaattaatt
     7321 catcataact ttcgttcatt ggtaacttga tattcgaact taaaggtgtt gcagtaactc
     7381 caagaataaa ggcatttttg aacgaactca ataatttccg aaaggaattg tagtgtgctt
     7441 catcgataat taccaatcca atgttgtcga gatgcaattt ctcgtcattg attcggtttt
     7501 tcaaggtttc gaccatcgct acaaaacaag aataatcgtt ttggtctgga agttctttga
     7561 ctttgctatt gatgattttg tttttgacat caaaaccttt gagcattttt gaagtttgct
     7621 tgcacaactc aattcgatgg gtcaaaacga ctactttttt attgtgtttt gataagtatt
     7681 gtctaacaat ctcagaaaaa acaacagttt ttccaccacc agttggcaat tgatacagta
     7741 aatgataatt gggagacgca ttgtctaagc gctcaaaaat ggattcaata tctccttttt
     7801 gatacgaata aagttctttc ttatcttcaa tttgtgtttc tagagtgttt tgagacattg
     7861 tatttattgg atttttgcaa aaatacacct aaaaaacagt taaatcacta aataatcgtg
     7921 taaaaaaaga attgggatta aataaaatcg tctatataat tgagaactag tattcaaaac
     7981 gttctaaaat ggcgtcaaat tcttggaaat taaaccactt ttggttaaaa gtctccttgt
     8041 ttatggcgat aattcgagtt ttgaattcgc ttaaaattcc attggcaatc ataaaactta
     8101 cagcctgact gaaagaattg agcatacttt tataaaacaa ttccgatttt atgatttttt
     8161 cggaggaaaa agtttgagca atttcaattg aatagagcat tacatcagca ataataaaag
     8221 gatctacccc caaaagcata aattgcttga tgtatttttg agccacagaa cggcgcattt
     8281 tgggttttcg agctttgcca gaagcagctc ttgcaggaaa atattcgttc gaaattttaa
     8341 tttttgattc ccttagtagt gtgtcttctt ttggattaaa aacaaaatca taatagtttt
     8401 ttacaggaac gaatttttca tacaactcaa taatttgctc ttcgagttgg gattttgtga
     8461 gttcgtttaa gtattttttt aaatcgcgtt tgctcatgga ttggttttat aaatgcaaaa
     8521 tgttaattgt ttcagaaatg acattcttaa aatcaacgat aatcattagt tttgccgtac
     8581 caaaaattaa aagtcatttt atgctaaaag tattcaaaaa catcgctatc ctagaaggta
     8641 tctcttattt agttttattc tttaatatgc tttatatcaa acattctaat attgaacttt
     8701 accatagttt gttgaatcca ttagggatga ctcacggtct gctgtttata ggatatgtac
     8761 ttttggcggt ttggctaaaa aaaatccaag aatggaataa ccaaactttg attattattt
     8821 taggagcctc actacttccc tttgcc
//
LOCUS       DAOSNN010000094         8661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8110_length_8661_cov_5.372298, whole genome shotgun sequence.
ACCESSION   DAOSNN010000094 DAOSNN010000000
VERSION     DAOSNN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8110_length_8661_cov_5.372298"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLQ70_06235"
     CDS             <1..909
                     /locus_tag="PLQ70_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQE34251.1"
                     /translation="PFVYATAIEQLGMSPCDTILDGPFMIRKGRHHVTEDWEPRNSNN
                     QYRGMVTLKQGLANSINTISAKLIDKTGPEAVIELTHKLGVKSDIPAQPSIALGAVDI
                     TVEDMVAAYGTFANQGVYVKPQFISRIEDKSGVVIYEPIPESHDVLNKDIAFAVIKLL
                     EGVTESGSGARLRTQGGGSGDNRWTGYPYMFTNPIAGKTGTTQNQSDGWFMGMVPNLV
                     TGVWVGCEDRSARFKSLTYGQGATAALPVWGYFMKLCYEDENLKVSKEEFDRPANLSI
                     KVDCYTRPKVVKDTTAVEQETEEFDL"
     gene            1077..1781
                     /locus_tag="PLQ70_06240"
     CDS             1077..1781
                     /locus_tag="PLQ70_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HQE34252.1"
                     /translation="MINKKVNTVQEALQGIENGMTLMLGGFGLCGIPENSIAELVKKE
                     TKDLTCISNNAGVDDFGLGLLLQKHQIKKMISSYVGENAEFERQMLSGELEVELIPQG
                     TLAERCRAAQTGIPAFFTPAGFGTEVAIGKETREFNGKMHVMELAFKADFAIIKAWKG
                     DTAGNLIFKGTARNFNPCMAGAAKITIAEVEELIEAGTLDPNQIHTPGIFVQRIFQGE
                     KYEKRIEKRTTRKPID"
     gene            1815..2159
                     /locus_tag="PLQ70_06245"
     CDS             1815..2159
                     /locus_tag="PLQ70_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009418620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQE34253.1"
                     /translation="MEKENIIVSKTIDFSLEIISFCEILEQNRKFVIANQLLKSGTSI
                     GANVHEAQNAESRADFIHKIKIAAKEIEETKYWLVLCEKSPNYPFEPKLKSKINEIGL
                     IVYKIISSSKSR"
     gene            2200..2856
                     /locus_tag="PLQ70_06250"
     CDS             2200..2856
                     /locus_tag="PLQ70_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002663177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HQE34254.1"
                     /translation="MLTKEDIAKRIAQEVKDKYFVNLGIGIPTLVANYIPKGIEVEFQ
                     SENGVLGMGPFPFEGEEDADLINAGKQTITTLKGASFFDSAMSFGMIRGQHVDLTILG
                     AMEVAENGDIANWKIPEKMVKGMGGAMDLVASAENIIVAMMHVNKAGESKILKKCTLP
                     LTGVGCVKKVVTELAVLELTPKGFKLLERAPGISVEHIMASTEAELIIDGEIPEMVID
                     "
     gene            2975..4054
                     /locus_tag="PLQ70_06255"
     CDS             2975..4054
                     /locus_tag="PLQ70_06255"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQE34255.1"
                     /translation="MKKTVSFYAMHLCICLAFMILPYLLTASGSVFSLPDLSGSGHDR
                     LYFFTYFTLLIFFYLNYYYLIPKVYFSNERVVYFFLIILFFLFFLWMSVFFDNPERNF
                     LDFRPVFSFKEDFRPPTHGNFSPSKHFNPKPMGGPPSQYGHTILVYLIGVVTSLLIAI
                     NIQLRKVEEDKMKSELSFLKAQINPHFLFNTLNSIYSLAIKKDDKTADAVVQLSELMR
                     YIITNANDDVIALEKEINYINNFVELQKTRLGNTVNVEYKVEGNPYGKSITPLILISF
                     IENAFKHGVNPNQDSEIVIKIDIVNNFLTLFVSNHKVENLQSDGGIGLQNTIERLTLL
                     YPDNHELKIDDSPKKYTVTLTLKVG"
     gene            4051..4740
                     /locus_tag="PLQ70_06260"
     CDS             4051..4740
                     /locus_tag="PLQ70_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQE34256.1"
                     /translation="MIKAIALDDEPPALDVLESFCDKIDVIDLQKKFTKSDDAFKYLK
                     KYPVDLLFLDINMPSISGIDFYKKLPHKTMVIFTTAYSEYAVEGFTLSATDYLLKPFS
                     FSRFQQAVEKAYSQWKIQNQNPEQNYLFIRADYSLIKILISDILFIEGLDDYLKIHIQ
                     NQKTIVARMTLKVILEKLPATEFARVHRSFIVPISKIEKVRNKIIFIQEEEIPVSASY
                     ETAFFTLLNKD"
     gene            4878..5633
                     /locus_tag="PLQ70_06265"
     CDS             4878..5633
                     /locus_tag="PLQ70_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HQE34257.1"
                     /translation="MERKEFLRGLGLAGLGALTIPVIKACSSNDDDASSTSTTDTTQA
                     TNTGGSTNTSCSVTSSETAGPFPTKSPSSLVTANIVSDRTGVGFTINITIKNTNASCA
                     VLAGAIVDIWHCDKDGYYSEYGGTSMQSADFTSAHFLRGRQTTNANGLVSFTSIFPGW
                     YTSRATHIHVHIYNSSGTSLLVTQIAFPEGSGSAVALVNASTANGYTKGLSGYTYNAS
                     DNVFSDSVANEMSTVTGSVAAGFILTHTINVAG"
     gene            5721..6410
                     /locus_tag="PLQ70_06270"
     CDS             5721..6410
                     /locus_tag="PLQ70_06270"
                     /inference="COORDINATES: protein motif:HMM:NF037012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34258.1"
                     /translation="MIQQHLAQIYKADFRGETTSESFKRLSTFNFENYQNDSRKPFGS
                     LQFLNEEILAPKHKQTTAASSNIELFILPLFGGIEYKDSLGNENFIRVEQIQRFTVQK
                     GMSFEVSNPFDENVSYLQMGFDFKNSGFQNNSKPVDFDLTQKNQLISLFKNRNSQGFI
                     GIYEGRKEGFYQLKNTSNGIFVFVINGTFEIENRLLESKDGLSLQQIDTIEWEALSEN
                     AILLVLEISLK"
     gene            6417..6923
                     /locus_tag="PLQ70_06275"
     CDS             6417..6923
                     /locus_tag="PLQ70_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34259.1"
                     /translation="MNKVIIRLAYRIIIDNSSTFLWDKYVWEDTYKEYLMQNQQFNSK
                     EKPKNTFRELLSENEKAVQLHYLVGIAANGYIEQLKGNFHRVTDVLGNNHFPFVNYKL
                     DIINTDITDISKHKVGITFYSPLLTYLGVVENHYLVSKTTDDVDGLETIMFPVQPRLS
                     ICYYEKKT"
     gene            complement(6994..8595)
                     /locus_tag="PLQ70_06280"
     CDS             complement(6994..8595)
                     /locus_tag="PLQ70_06280"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HQE34260.1"
                     /translation="MDFIYQDPYPILKDDTQYRKITSDFVKVEKLGDREILTVDPKGL
                     ELLAQEALKDVSFMLRTSHLEKLKAILDDPEATDNDRFVAYNLLQNAVVAIDGELPSC
                     QDTGTAIVMAKKGENVYTGVDDAEWLSRGIFNTYQEKNLRYSQIVPISMFEEKNSGSN
                     LPAQIDIYAKKGATYEFLFLAKGGGSANKTYLYQQTKSLLNEKSLDAFIRAKIMDLGT
                     SACPPYHLALVIGGTSAEANLAAVKKASAGYFDNLPTSGNMAGQAFRDLEWEKRVQLI
                     CQESQIGAQFGGKYFTHDVRVIRLPRHAASCPVGLGVSCSADRNIKGKITKDGIFVEQ
                     LEVNPKQFLPATPPHLEEAVEVDLNQPMADILKKLSQYPIKTRLKLNGTLIVARDIAH
                     AKIKELLDAGKPMPEYFKNHPIYYAGPAKTPEGMPSGSFGPTTAGRMDVYVEEFQRNG
                     GSMVMLAKGNRTKDVMNACKTYGGFYLGSIGGPAAILAKENILSVEVVDFEELGMEAV
                     RKITIKDFPAFIITDDKGNDFFANL"
BASE COUNT         2856 a         1622 c         1542 g         2641 t
ORIGIN      
        1 ccgtttgtat atgcaaccgc tatcgagcaa ttaggaatgt ccccttgcga taccattctc
       61 gacgggcctt ttatgattcg caaagggcgt catcacgtaa ccgaagattg ggaaccaaga
      121 aattcgaaca atcagtatcg tggaatggta acgctaaaac aaggactggc caattctatc
      181 aacaccattt cggcaaaatt aatagacaaa acaggtccag aagctgttat cgaattaacc
      241 cataaattag gtgtaaaatc tgatattcca gctcagcctt ctatcgcatt aggcgctgtt
      301 gacattaccg tagaagacat ggtggcagct tacggaacct ttgccaacca aggtgtttat
      361 gtaaaacctc aattcatttc tcgaatcgaa gacaaaagtg gcgttgtaat ctacgaaccc
      421 attccagaat ctcacgatgt attgaacaaa gatatcgcat ttgccgtaat caaattactc
      481 gaaggcgtta ctgaaagtgg ttcaggagct agattaagaa cacaaggtgg cggtagtgga
      541 gacaatcgct ggacaggata tccgtatatg tttaccaatc caattgctgg aaaaacagga
      601 acaactcaaa accagtcgga tggttggttt atgggaatgg tacctaactt agttactgga
      661 gtttgggtag gttgcgaaga ccgttcggca cgtttcaaaa gcttgactta tggacaagga
      721 gcaacagccg cattacccgt ttggggctat tttatgaaac tttgttacga agatgaaaac
      781 ctcaaagttt ccaaagaaga attcgataga cctgccaatc tttctataaa ggtagattgt
      841 tacacccgcc caaaagttgt aaaagacacc acagccgttg aacaagaaac cgaagaattc
      901 gatttatagt ttctaggagc tgtttcctgc tgtacactgt atcttttgtg gcgaaccccg
      961 ccacaaaagg atgccgttcc catcagggct agggcatttg tttcaaaata accaatatcg
     1021 aaaccaaaca actttgaaaa cttaatttta atggtttaaa aaatcattgt ataattatga
     1081 tcaacaaaaa agtaaatacc gttcaagaag cactccaagg aatcgaaaac ggaatgaccc
     1141 tgatgttggg cggttttgga ttgtgtggca ttccagaaaa ctccattgcc gaactggtca
     1201 aaaaagaaac caaggatttg acctgcattt ccaacaatgc cggtgtcgat gattttggtc
     1261 tcggattgct tttgcaaaaa caccaaatca aaaaaatgat ttcttcctat gtgggagaaa
     1321 atgcagaatt tgaacgccaa atgctctccg gcgaactcga agtagaactc attccacaag
     1381 gaactttagc ggaaagatgt cgcgccgccc aaaccggaat tcctgctttt ttcacaccag
     1441 ccggtttcgg aaccgaagtc gccattggca aagaaacccg agaattcaac ggaaaaatgc
     1501 acgtgatgga attggccttc aaagctgatt ttgccattat aaaagcttgg aaaggcgaca
     1561 cagcaggaaa cctcatcttc aaaggaacag cccgaaattt caatccctgc atggcaggtg
     1621 cagctaaaat taccatcgca gaagtcgaag aattaatcga agccggaacc ctcgacccta
     1681 accaaattca cactcccgga atcttcgtgc aacgcatctt tcaaggagag aaatacgaga
     1741 agaggattga gaagcgaaca acaagaaaac caattgatta atgtgaaaat gtatcaatta
     1801 attcaaataa ttatatggag aaagaaaaca taatagtttc aaaaacaatt gatttttcat
     1861 tagaaattat ttctttctgt gaaattttag aacaaaacag aaaatttgta attgcaaatc
     1921 aattattaaa atctggaact agtattggtg caaatgttca cgaagctcaa aatgccgaaa
     1981 gtagagctga tttcatccat aaaattaaaa tagctgcaaa agaaatcgaa gaaacaaaat
     2041 attggttagt cctatgtgaa aaatcaccga actatccctt tgaacctaaa ttgaaatcta
     2101 aaattaatga aattggactt attgtttata aaatcataag tagtagtaaa tctagataat
     2161 tggtaaatta ttatattgat taattcacac attattttta tgctaacaaa agaagacatc
     2221 gccaaacgca ttgcccaaga agtcaaagac aaatactttg taaacctcgg aattgggatt
     2281 ccaaccttgg tggccaatta tatcccaaaa ggaattgaag tggaatttca gagtgaaaac
     2341 ggcgttctcg ggatgggacc tttccctttt gaaggagagg aagacgctga tttaatcaat
     2401 gctggaaaac aaaccatcac gactttaaaa ggcgcatctt tctttgactc ggcaatgagt
     2461 ttcggaatga ttcgtgggca acacgtagat ttgaccattc ttggtgcgat ggaagttgcc
     2521 gaaaacggtg atattgccaa ctggaaaatc cccgaaaaaa tggtcaaagg aatgggcggc
     2581 gcaatggatt tggtagcatc tgccgagaat attatcgtgg caatgatgca cgtcaacaaa
     2641 gcaggagaaa gcaaaattct taaaaaatgt acgttaccct taacaggcgt tggctgcgtg
     2701 aaaaaagtcg taaccgaatt ggccgttctc gaactgactc caaaaggttt taaactattg
     2761 gaacgcgcgc ctggtatttc tgtagaacat attatggctt ccaccgaagc tgaattaatt
     2821 atcgatggag aaattccgga aatggtgatt gattaacaac acttttccat aagtggttct
     2881 atactttctt aaactaccgc aacaattacc cattttacct aaataatttt attgaaaacc
     2941 ctaacaatca tacttttgaa acaataattt cactatgaaa aaaacagttt ctttttatgc
     3001 tatgcattta tgtatttgtt tggcatttat gattttacct tatttgctta cagcatcagg
     3061 aagtgttttt agtttgccag atttatcggg tagtggacac gacagactgt acttttttac
     3121 ctattttacg ttattgattt tcttctattt aaactattat tatttaattc ctaaagtcta
     3181 tttttctaat gaaagagtag tgtacttttt tctcatcatc ttgttttttt tattttttct
     3241 ttggatgtct gtttttttcg ataatccgga gcgtaatttt ttggatttta gacctgtatt
     3301 ttcctttaaa gaagattttc ggccaccaac acacggaaat ttttcccctt ccaaacattt
     3361 taatcctaaa ccaatgggag gcccaccttc tcaatacgga cacacaatat tagtttattt
     3421 aatcggtgtt gttaccagct tattgattgc cattaatatt cagcttcgaa aagtagaaga
     3481 agataaaatg aaatcggaat tgtctttttt gaaagcacaa attaatcctc attttttgtt
     3541 caataccttg aatagtattt attcattagc cataaaaaaa gacgataaaa ctgccgatgc
     3601 cgtggtacaa ttatccgaat taatgcgcta cattattacc aatgccaacg atgatgttat
     3661 tgctttagaa aaagaaatca attacattaa caattttgta gaacttcaaa aaacacgttt
     3721 gggaaataca gtaaatgtag aatataaagt agagggaaat ccttatggca agagtatcac
     3781 accactcatt ttaatttcgt ttattgaaaa tgccttcaag cacggggtaa atcccaatca
     3841 ggattcagaa attgttatta aaattgatat tgtaaataat tttttgactt tatttgtttc
     3901 caatcataaa gtagaaaatc tacaatcgga tggaggcatt ggattgcaaa atactataga
     3961 aagattgacc cttttgtatc ccgataatca cgaattaaaa attgacgaca gtccaaaaaa
     4021 atatactgta actttaaccc taaaagtagg atgataaaag caattgcact agatgacgaa
     4081 ccgccagcac tggatgtttt agaaagtttt tgcgacaaaa ttgatgttat cgacttgcaa
     4141 aaaaagttta ctaaatcgga tgatgctttc aaatatttga aaaaatatcc ggtagattta
     4201 ttgtttttag atatcaatat gccttctatt tcgggaattg atttttataa aaaactgcca
     4261 cataaaacga tggttatttt tacaacggct tattctgaat atgctgtcga aggatttacg
     4321 ttgagtgcga ccgattatct tctgaaacct ttttcattct cccgatttca gcaggcggta
     4381 gaaaaagcct attcgcaatg gaaaattcaa aatcaaaatc ccgaacaaaa ctaccttttt
     4441 attcgggcag attacagttt gattaaaatt ctaatttcgg atatattgtt catcgaagga
     4501 ctggatgatt acttgaaaat tcatattcaa aaccaaaaaa caattgtcgc cagaatgact
     4561 ttgaaagtaa tattggaaaa actcccagcg actgaatttg cacgggtgca tcgatcgttt
     4621 atagttccta tttctaaaat agaaaaagtt cgaaataaaa tcatcttcat tcaggaagaa
     4681 gagattccag taagcgccag ttatgaaact gcttttttta ctttactcaa caaggattaa
     4741 tttttattgt taaaaaatta caaattcaac tttattttca tccatttacc tcataaaaac
     4801 aatctattac ctcataaatt tataaacttt tgtgagttta aatactttta tatccgtaac
     4861 cttaaaatac tattattatg gaaagaaaag aatttttaag aggattaggt ttagctggtc
     4921 ttggtgcttt gaccattcct gtaattaaag cctgtagtag taatgatgat gatgcttctt
     4981 ctacatcaac aacagacact acccaagcta caaatacagg aggatcaaca aatacttctt
     5041 gttcggttac ttcgtcagaa accgctggcc cgtttccaac caaatcacct tcttcattag
     5101 ttacggcaaa tattgtaagt gatagaacag gtgtaggatt cacaatcaat atcaccatca
     5161 aaaataccaa tgccagctgt gcagtattgg ctggagccat tgtcgatatt tggcattgcg
     5221 acaaagacgg ttattattct gaatacggag gaacctcaat gcagtcagcc gattttacaa
     5281 gcgctcattt cttaagaggc agacaaacaa ccaatgctaa cggactggtt agttttacct
     5341 ctatttttcc gggttggtac accagtagag caactcacat tcacgtacat atttacaact
     5401 cttcgggaac ttcgttactg gttactcaaa ttgcttttcc agaaggttca ggaagtgccg
     5461 tagcactagt caatgcgtca acagccaatg gatataccaa aggattatcg ggttatacct
     5521 ataatgcttc tgataatgtg ttttctgata gtgtagccaa tgaaatgtcc accgttaccg
     5581 gaagtgtagc cgctggattt attttaacac atactataaa tgttgcaggt tgatttttga
     5641 attagtaaaa aaatgacgcc aaaacctgta agatttctag attttgcagg taattttttt
     5701 aattgtcaaa agctatttat atgatacaac agcatcttgc ccaaatatat aaagccgatt
     5761 ttcgaggaga aaccacatcc gaaagtttta aacgcctgtc tactttcaat ttcgaaaact
     5821 atcaaaatga ttccagaaaa ccatttgggt ctttgcaatt tttgaatgaa gaaatactgg
     5881 ctccaaaaca caaacaaaca actgctgcat catctaatat agagttattc attttacctc
     5941 tttttggcgg tatagaatac aaagacagtt taggaaacga aaattttata agagttgaac
     6001 aaattcaacg gtttacagtc caaaaaggaa tgtcctttga agtttctaat ccttttgatg
     6061 aaaatgtgag ttatctacaa atgggtttcg actttaaaaa ttcaggtttt caaaacaatt
     6121 ccaagcccgt tgattttgat ttaacccaaa aaaatcaatt gatttctctc tttaaaaata
     6181 gaaattcaca gggttttatc ggaatttacg aaggcagaaa agaaggcttt taccagctta
     6241 aaaatacatc aaatggcatt tttgtttttg taatcaacgg tacttttgaa atcgaaaacc
     6301 gattgttaga atctaaagac ggattgagtt tgcaacaaat agataccata gaatgggaag
     6361 cattgtccga aaatgcgatt cttttagtat tggaaatttc tttaaaataa accattatga
     6421 acaaagtcat aatacgatta gcctaccgaa ttataatcga caactcctct acattccttt
     6481 gggacaaata tgtttgggaa gacacctaca aagaatattt aatgcaaaac cagcaattta
     6541 actcgaaaga gaaacccaaa aacacattca gagaattatt gtcagaaaat gaaaaagcag
     6601 tacagttgca ttatttagtg ggcatagccg caaatggtta tatcgaacaa ttaaaaggaa
     6661 attttcatcg cgttaccgat gtattgggaa acaatcattt cccctttgtc aattacaagc
     6721 tagatattat caacactgac atcaccgata tttcgaaaca caaagtcggg attacttttt
     6781 attcgccttt gcttacttat ttaggcgtgg tagaaaacca ttatttagtt tcaaaaacca
     6841 ccgatgatgt agatggtttg gagactatta tgtttcctgt acaaccccgt ttatctatat
     6901 gttattacga aaagaaaact taaattgact ttcttaaaat aaaatgaccg ccaacattga
     6961 cggtcattta aaaataaact ctttttattt tatctacaaa ttcgcaaaga aatcattccc
     7021 tttatcatct gtaataataa aagcaggaaa atctttaatg gtgattttac gaacagcttc
     7081 catacctaat tcttcaaagt caactacctc aaccgaaaga atgttttctt tggctaaaat
     7141 cgccgctggt cctccaatgg aacctaaata gaatccaccg taagttttac aagcattcat
     7201 cacatctttg gttcggtttc ctttggccag cattaccata ctgccgccat ttctttggaa
     7261 ttcctcaaca tacacgtcca tacgtcccgc agtagttggt ccaaaacttc ccgatggcat
     7321 tccttccggt gttttggcag gtcctgcgta gtaaattggg tggtttttga aatattctgg
     7381 cattggttta ccagcatcca ataattcctt gattttggcg tgggcaatgt cacgggctac
     7441 aatcaaagtt ccattcaatt tcaaacgggt tttgattgga tattgcgata attttttaag
     7501 aatatcggcc attggttggt tcaaatccac ctcaaccgct tcttctaaat gtggtggcgt
     7561 tgcaggcaaa aattgttttg gattgacttc caattgttct acgaaaatac cgtctttggt
     7621 gatttttcct ttgatatttc tatcggccga acaagaaact cccaatccaa ccggacaaga
     7681 agcggcgtgg cgtggcaaac gaatcactcg aacatcgtgg gtgaaatatt ttccaccaaa
     7741 ctgcgctccg atttgacttt cttggcaaat taactgtact cttttttccc attccaaatc
     7801 acggaaagcc tgaccggcca tatttcctga tgttggcaaa ttatcaaaat aacccgccga
     7861 tgcttttttc acggcagcca aattcgcttc ggcagaagtt cctccaatca ctaaagccaa
     7921 gtgatagggc ggacaagccg aagttcctaa atccattatt ttggcacgga tgaaagcatc
     7981 caaagatttt tcgttcagca aggattttgt ttgttggtac aaataggttt tgttggcaga
     8041 accaccgcct tttgccaaga acaaaaactc ataagtagct ccttttttgg cataaatatc
     8101 aatttgtgcc ggaagattcg aacccgaatt tttttcttca aacatactaa ttggtacaat
     8161 ttgagaataa cgaaggtttt tttcttgata ggtattaaaa attcctctgg aaagccattc
     8221 ggcatcatca acgcctgtat atacgttttc gcctttttta gccattacaa tcgccgttcc
     8281 ggtatcttga cacgaaggca attccccatc aatagccact actgcatttt gcaataaatt
     8341 ataggctaca aatcggtcgt tgtcggttgc ttctggatca tccaaaatgg cttttagttt
     8401 ttctaaatgc gaagttcgca acataaacga aacatctttc aatgcttctt gtgccaacaa
     8461 ttctaagcct tttgggtcaa cagtaagaat ttctctgtcg cctagttttt ctacttttac
     8521 aaaatcggaa gtgattttgc gatattgagt atcgtctttt aaaattggat aaggatcctg
     8581 gtatataaag tccataattt gagttttttt agaacgtcaa agataagaat tgtagaaaga
     8641 aatctttgcg atttatatca a
//
LOCUS       DAOSNN010000095         8611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8159_length_8611_cov_3.847359, whole genome shotgun sequence.
ACCESSION   DAOSNN010000095 DAOSNN010000000
VERSION     DAOSNN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8159_length_8611_cov_3.847359"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLQ70_06285"
     CDS             complement(<1..194)
                     /locus_tag="PLQ70_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HQE34261.1"
                     /translation="MSELKLMKGNEALAEAAIRAGVDAYFGYPITPQTEIIEYLMTAR
                     PENRTGMVVLQAESEIAAIN"
     gene            complement(214..444)
                     /locus_tag="PLQ70_06290"
     CDS             complement(214..444)
                     /locus_tag="PLQ70_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQE34262.1"
                     /translation="MAKVRGAIVVNTQICKGCEVCVPVCQEKVIGISSDVNRKGYHYA
                     YMKNPEACNGCTNCGTVCPDRAIAVYRVKVAT"
     gene            complement(669..1172)
                     /locus_tag="PLQ70_06295"
     CDS             complement(669..1172)
                     /locus_tag="PLQ70_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34263.1"
                     /translation="MRKLALIIAFLLTGLFVNAQVKKIVISVDESVDVASFDLDCSKS
                     FPTLAKGLKYNVTRHEYKGEELKNTYHLLLVMDGFFTKTLNKKMTISVVFSDDTTFSE
                     IETIENDGYFDGACTLKMKSPPENALTLDLKKVIVSTGTKDVVYTISAQKAADFKKNI
                     QNIVTAK"
     gene            complement(1187..1843)
                     /gene="fsa"
                     /locus_tag="PLQ70_06300"
     CDS             complement(1187..1843)
                     /gene="fsa"
                     /locus_tag="PLQ70_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQE34264.1"
                     /translation="MKFFIDTANLAQIKEAQALGVLDGVTTNPSLMAKEGITGKNNIL
                     KHYVDICNLVDGDVSAEVIALDFDGMIKEGEELVDLHEQIVVKLPMTKEGVMAAKYFS
                     DKGIKTNVTLVFSAGQALLAAKAGATYVSPFIGRLDDISTDGLALIEEIRLIYDNYGY
                     ETQILAASVRHTMHIVNCAKIGADVITGPLSAISGLLKHPLTDIGLAQFMADFEKGNK
                     "
     gene            complement(1882..2685)
                     /locus_tag="PLQ70_06305"
     CDS             complement(1882..2685)
                     /locus_tag="PLQ70_06305"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE34265.1"
                     /translation="MSKVVLITGGSSGIGKSIGEFLHHKGFIVYGTSRNPDRVLNSVF
                     PLIALDVRSTESIQLAVAKIIASSGRIDIVINNAGVGITGPLEEIPTEEIRNNFETNF
                     FGPIQVMKAVLPQMRYQKSGMIINITSIAGYMGLPYRSVYSASKGALELITEALRMEV
                     QSFGIQITNVAPGDFATNIASGRFHAPLFKDSAYEIPYGNTLKMMDEHVDSGSNPNEM
                     AEAVYQIIQEKNPKIHYKVGAFMQKFSIVLKRVLPDKVYEKMLMNHYKL"
     gene            2945..3664
                     /locus_tag="PLQ70_06310"
     CDS             2945..3664
                     /locus_tag="PLQ70_06310"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HQE34266.1"
                     /translation="MKKYYLHNGTESSGPFDIGELKFKKITPSSPVWFEGMLHWKTAG
                     EIPELNQLFVVNPPPFAPFMTPEPETKTDTERNTIKILGLSKNTFWIVCAAIIILVGI
                     TVLRALEEKRSQELKEKNHKTEIENYQLELKQKELEDEKIQAAIQEKIDADRMAAELK
                     QKNNNRLLEIEQLLVNSQNNLNTYENKLTNAAEFKLLRTAAEKKEQMDLLRNQIDSIK
                     TNISKLKNESDQLKLELEKIP"
     gene            3930..7355
                     /locus_tag="PLQ70_06315"
     CDS             3930..7355
                     /locus_tag="PLQ70_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQE34267.1"
                     /translation="MSIKIAISHKTTYKFDRSVQLFPHIFRLRPAVHSRTQIEGYTFK
                     ISPEEHFINWQQDPFGNYQARVVFPNKIKELKVEVEVIAKLQVINPFDYFVEEYAEKF
                     PFKYDSTLKDELIPYLKLSEESPVFLKFIEKIKLNDSIQINDFLVYINQAVYKELTYN
                     LRMEVGVQTPEETLRIKSGSCRDFAWLLIHILRHFGLAARFVSGYIVQLAPDIKSLDG
                     PSGPEKDFTDLHAWAEVYIPGAGWIGLDPTSGLFASEGHIPLCCTPHYESAAPVTGAT
                     EKCEVSFEFENIVTRIHEDPRVTKPYSEKQWEEIMEVGNAVEKDLIEGDVRLTMGGEP
                     TFISIDDFEGDEWNTAADGPLKRKLAYDLALRLKKRFAHGGLLHFGQGKWYPGELFPR
                     WQYGLYWRKDGLPLWKNDDLVAKEGETKYNFHDAEKFALELTKFLGIDTKNLLPTYED
                     PIYWALEEGKLPVNLDPLKVNLKDSIERHNLAKVLEKGLNNPSGFVLPIRKDYAQDNW
                     ESCAWEFRRGNCFLIPGNSPIGLRLPLDSLPKTSKNKRETVVDRSLFEDLPALGDPTE
                     KINNRYGTISKIFEVVKKVLSKKEEKKKAEESLLFEVDTFITALCIEERDGMLYIFLP
                     PTDYLEDYIDLITSIEITAEKLQMPVRIEGYQPKTDYRVEKMMVTPDPGVIEVNVHPA
                     KSWQEIVDNTTALYEEAFLSRLGTDKFMVDGRHTGTGGGNHVTLGASKPEDSPLLRRP
                     DLLRSLITYWQHHPVLSYLFAGPFIGPTSQAPRIDEGRDERLYEMEIAFEQIPKDKEV
                     PFWMVDRIFRNLLTDITGNTHRTEFCIDKLYSPDSSTGRLGILELRAFDMPPHKHMNL
                     VQNLLVRALVAKFWKNPYEKKLIRWGTELHDKFLLPHFAYLDMIDVVNDLKDAGYNFD
                     ISWFDPFFEFRYPHHGNVTIDNIQLEIRLGIEPWHVLGEELSSSGTSRFVDSSLERLQ
                     VKVSGIVPDRHILLCKGCRIPLRSTGTKGEYVAGIRYKSWNPPSALHPNIGIDVPLVF
                     DLVDTWNNKVIGGCTYFVTHPGGRSFDTFPINSFEAESRKISRFWDFGHTPSSTVEET
                     ENEKERETIVNSNATSRFLVESKSELRPDTPIELINPEYPYTLDLRHYWKAKE"
     gene            7443..>8611
                     /locus_tag="PLQ70_06320"
     CDS             7443..>8611
                     /locus_tag="PLQ70_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HQE34268.1"
                     /translation="MIQDISLGLLNTYKESINSYDEVIDQNGQIKPYWKGLFDTLESI
                     GIEELNLRNEEIIKKLKENGVTYNIYDSNKNSNRAWKLDPIPFLIHESEWKTIEKGLK
                     QRARLLDLILKDLYGPQTLIKNAIIPAELVFDNSGFLLPCFDIRQKHNKQLLNYAVDL
                     ARGPDGKMWLLDNRTQAPSGAGYALENRIVMSKVIPELNKKTYRKRLSPYFSQMQQTV
                     DTLGNNSNENPNVVFLTPGPGNETYFEHVYLSSYLGYTLVQGNDLLVRDGFVWLKSID
                     QLERVDVIIKRLDDTWCDPLELRKDSLLGIPGLLQVIRLGNVSVVNPPGTSILENYGL
                     MAFMQNACKFLLKEPLLMNSVATWWCGQAKELNFVLTNLPKLIIKKTNRKQGFRS"
BASE COUNT         2938 a         1503 c         1476 g         2694 t
ORIGIN      
        1 atatttatcg ccgcaatttc actttcggct tgcaaaacaa ccattcctgt tctattttct
       61 ggtcgagcag tcattaaata ttcgataatt tcggtttgtg gagtaattgg atagccaaaa
      121 tatgcatcaa cccctgctct gatggcagct tctgctaatg cttcatttcc tttcattaat
      181 tttaattcag acattttttt aaaatataaa ggattatgtt gcgactttta ccctataaac
      241 ggcaatggct ctatctggac aaacagtgcc acaattggtg caaccattac aagcttcggg
      301 gtttttcata taagcataat gatagccttt tctgttgaca tcgcttgaga ttccaatgac
      361 tttttcctga caaacaggaa cacaaacttc gcaacctttg caaatttgag tatttacaac
      421 tatagcacct cttacttttg ccattttaat aaatttagat gtaattattc aatcataatt
      481 ttattattct caaatttaca acttatccat tgcaaataaa atgacaaata tcatattatt
      541 tagatttttg gacacaaaaa aataatttca atttaaaaca ttttccaaac aaaaaagcca
      601 ctaaattttg aatttagtgg cttttaaaat ttaaaatcaa ctcaatgatt ataagtaaga
      661 tctaactttt atttagcagt aacaatgttc tgtatattct ttttaaaatc agctgctttt
      721 tgagcagaaa ttgtataaac tacatcttta gtaccagtac ttacaataac ttttttcaaa
      781 tccaaagtta aagcattttc tggtgggctt ttcattttta gcgtacatgc tccatcaaaa
      841 taaccatcgt tttctatggt ttcaatttct gaaaaagtgg tatcatcact aaatacaact
      901 gaaatagtca tctttttatt tagtgtttta gtaaaaaatc catccatgac taataataaa
      961 tgataggtgt ttttcagttc ttcgcctttg tattcatgtc gagtgacatt gtatttcaga
     1021 cctttggcta aagttggaaa acttttacta caatccaaat caaaactggc tacatcaaca
     1081 gactcatcaa cagatataac aatttttttt acttgagcat tgacaaataa acctgtcaac
     1141 aagaatgcta ttattaaagc caattttctc atagatactt tgttttttat ttatttcctt
     1201 tttcaaaatc ggccataaat tgtgccaatc caatatcggt taatgggtgt tttaataaac
     1261 ctgaaatagc agaaagtggc ccagtaatta catcggcacc aattttagca cagtttacaa
     1321 tatgcatagt atgacggact gaagcagcaa gaatttgtgt ttcgtaaccg taattgtcat
     1381 atatcaatct gatttcttca ataagtgcca atccatctgt agaaatatcg tctaaccgac
     1441 caataaaagg agaaacataa gtggcacccg ctttagcagc caataaagct tggcctgcag
     1501 agaacactaa ggttacatta gtttttattc ctttatccga aaaatatttt gcagccatta
     1561 caccttcttt agtcattggc aattttacca caatttgttc gtgtaaatca accaattctt
     1621 caccttcttt gatcattcca tcaaaatcca gcgcgattac ttcggcactt acatctccat
     1681 ctacaaggtt gcaaatgtca acataatgtt tcaaaatatt attttttccg gtaatacctt
     1741 cctttgccat caaggatgga ttggtagtta ctccgtctaa aacgcctaat gcttgggctt
     1801 ctttaatttg agctaaatta gctgtatcga taaaaaattt cattgtttaa agtttatagt
     1861 tactattatt gattagtaaa attacaattt ataatggttc atcagcattt tttcatacac
     1921 tttatcaggc agaactcgct tcaaaacaat cgaaaatttt tgcataaaag cccccacttt
     1981 ataatgtatc ttaggatttt tttcttgaat tatttgataa actgcttctg ccatttcgtt
     2041 aggattactg ccgctgtcca cgtgttcatc catcattttc aaagtatttc catacggaat
     2101 ttcataggcc gaatccttga ataatggcgc atgaaaacgt ccagaagcaa tatttgtagc
     2161 aaaatctcct ggagctacat tcgtaatctg aattccaaat gattgtactt ccattcgcaa
     2221 cgcttcggta attaattcta aagcgccttt ggatgctgaa taaacactac gataaggcaa
     2281 ccccatataa cctgcaatag aagtaatatt gataatcatt cctgattttt ggtaacgcat
     2341 ttgtggcaaa accgctttca ttacctgaat tggtccaaaa aaattagttt cgaaattatt
     2401 tctgatttcc tcagtcggaa tttcttctaa agggccagta attccaacac cagcgttatt
     2461 tataacgata tctattcgcc cagaagatgc aataatttta gcaacagcca attgaataga
     2521 ttcagtactt ctaacatcta aagcaattaa tggaaatact gaatttaaga cccgatctgg
     2581 atttctacta gttccgtaaa caataaatcc tttgtggtgt aaaaattcgc caatagattt
     2641 tccgattccc gaagaaccgc ctgtgattaa aacaacttta ctcatattat tattgaaccc
     2701 aacagctata gagatgggga ttttagtgaa ttttgattgt aaatttagta ttttttttca
     2761 aatacaaact gcaaaaacaa ttatgcccta agtgaaggac gtagcaaact actttttgaa
     2821 taacttcaac aagttataat aattgcaaag aataatagtt aaaattactt ttagatacaa
     2881 acccattttg aagtatcaaa aaatgattgt agatttgaaa aaatcgattt aatagcgtct
     2941 ttttatgaaa aaatactatc tacacaacgg aaccgaaagc agtggtcctt ttgatatagg
     3001 agaattaaag ttcaaaaaaa tcacaccctc tagccctgta tggtttgaag gaatgctaca
     3061 ttggaaaacc gctggagaaa tccccgaatt gaaccaactt tttgtagtaa acccgcctcc
     3121 ttttgctccg tttatgactc ctgaaccaga gacaaaaaca gatacagaaa gaaatacaat
     3181 caagatttta ggcttgtcca agaatacgtt ttggattgtt tgtgctgcga taattatttt
     3241 agttggaata acagtattga gagccttaga agaaaaaaga agtcaagaat taaaagaaaa
     3301 aaaccacaaa accgaaattg aaaattatca attggaacta aaacaaaaag aattagaaga
     3361 cgaaaaaatt caggcagcta tacaagaaaa aattgatgca gatagaatgg ccgcagaatt
     3421 aaaacaaaaa aacaacaata gacttttgga aattgagcaa ctattggtca actctcaaaa
     3481 taatttgaat acctatgaaa ataaactaac caacgcagct gaatttaagt tattgagaac
     3541 cgcagcagag aaaaaagaac aaatggattt gttgagaaac caaattgatt cgattaaaac
     3601 caatatatcc aaactaaaaa acgaatccga tcaactgaaa ctcgaattag agaaaatacc
     3661 gtaattgatt cgaatagcat taaatagcta aagcctattc ttttttggag taaatatgga
     3721 ataaaaaata agttacctca atttaaaata gagtgtacta caaaggattt ttacttgaat
     3781 tacattattc tcaaaaataa aactcaaaat tatcgattta aaggtattat cgaataaatt
     3841 tgataaataa ttatcatata aattgggtaa atggtgtttt tttttcaata tttgtataag
     3901 taaaattatt ctataaaaaa cacttttata tgtccataaa aatagccatt tcccacaaaa
     3961 caacgtataa atttgaccga agtgttcaat tatttccaca catctttaga ctacgtccag
     4021 cggtacattc gagaactcaa atagagggct atactttcaa gataagtcca gaagagcatt
     4081 ttatcaattg gcaacaagac ccttttggaa attatcaagc tagagttgtt tttcctaata
     4141 aaatcaagga attaaaagtg gaagttgaag ttattgccaa actacaagtc attaacccat
     4201 ttgactattt tgtagaagaa tatgctgaaa aattcccatt caaatacgat agtacactaa
     4261 aagacgaatt aattccttat ttgaaactaa gcgaagaaag tcctgttttt ttgaagttta
     4321 ttgaaaagat aaaactaaat gattctatac aaataaatga ttttttagtt tacatcaatc
     4381 aagctgttta caaagagctg acctataatc taaggatgga agttggggtg caaactccag
     4441 aagaaacctt aagaataaaa agcggatctt gtagggattt tgcttggctg ttgattcata
     4501 ttttgcgtca ttttggtctt gctgcgcgtt ttgtttctgg ttatatcgta caattagccc
     4561 cagatattaa atcacttgat ggtccatcgg gacctgaaaa agattttacc gatttacacg
     4621 cttgggcaga agtttatatt cctggtgcgg gctggattgg acttgaccct acttcgggac
     4681 tatttgccag tgaagggcat attccgttgt gctgtactcc gcattatgaa agtgcagctc
     4741 cagtaacggg ggcaacagaa aaatgtgaag tgagttttga atttgaaaat atagtcaccc
     4801 gaattcacga agatccaaga gtaaccaaac cctattctga aaaacaatgg gaagaaatta
     4861 tggaggttgg aaatgcggtc gaaaaagatt taattgaagg tgatgtccgg ctaacaatgg
     4921 gtggagaacc cacctttatt tctatcgatg attttgaagg cgatgaatgg aatactgctg
     4981 ccgatggtcc attgaaaaga aaacttgcct acgatttagc tcttcgatta aaaaaacgat
     5041 ttgctcacgg agggttgctg cattttggac aaggaaaatg gtatcccggc gaattatttc
     5101 cgcgttggca atatggattg tactggagaa aagacggatt gcctttatgg aaaaatgatg
     5161 atttagtagc caaagaaggt gaaaccaaat acaattttca cgatgccgaa aaatttgccc
     5221 tagagttgac caaatttctt ggaattgaca ctaaaaacct attaccaacc tacgaagacc
     5281 ctatttattg ggcattggaa gaaggaaaac ttcctgtgaa tttagatccc ttgaaagtaa
     5341 atttaaaaga ttctatcgaa cgccacaatt tagccaaagt cctagaaaaa ggcttgaaca
     5401 atccatcggg attcgtttta ccaattcgaa aagattacgc tcaagacaat tgggaaagtt
     5461 gcgcttggga attcagaaga ggcaattgtt tcttaattcc tggaaactcc cctattggtt
     5521 tgcgtttgcc attggattct cttccaaaga cttccaaaaa caaaagagaa accgttgtag
     5581 atagaagttt attcgaagat ttacccgctt taggagatcc taccgaaaaa ataaacaatc
     5641 gctacggaac catttcaaag atttttgaag tggtcaaaaa agtattgtca aaaaaagaag
     5701 aaaaaaagaa agccgaagaa tccttattgt ttgaagtaga tactttcatc acagccttgt
     5761 gtatagaaga acgtgatggc atgttatata tctttctacc gccaacagat tatttggaag
     5821 actatatcga cttaatcact tcgatagaaa ttacagccga aaaactacaa atgcctgtta
     5881 gaattgaagg ctaccaaccc aaaacagatt atcgagtaga gaaaatgatg gtaacgccag
     5941 atccaggtgt aattgaagta aacgtacatc cagccaaatc atggcaagaa attgtagata
     6001 ataccaccgc tttatatgaa gaagctttct tatctcgttt gggaaccgat aaatttatgg
     6061 tagatggtcg tcatactggg acaggcggtg gcaatcacgt aactttaggt gcttcaaaac
     6121 ctgaagatag tcctctgtta agaagacccg atttattgcg cagtttgatt acctattggc
     6181 aacatcatcc ggttttgagt tacctttttg cagggccttt tattggtcca acaagtcaag
     6241 ctccccgtat tgacgaaggt cgtgatgagc gtttatacga aatggaaatt gcttttgaac
     6301 aaatcccaaa agacaaagaa gttccgtttt ggatggtgga tcgaattttt agaaacctct
     6361 tgaccgatat tacaggaaat actcatcgca ccgagttttg tatcgataaa ttatactctc
     6421 cagattcctc aacaggacga ttgggaatat tagaattacg tgcttttgat atgccaccac
     6481 acaaacatat gaacttggtt caaaacttat tggttcgtgc cttggtggcc aaattctgga
     6541 aaaaccccta cgagaagaaa ctaattcgct ggggaactga attgcacgat aagtttttat
     6601 tgcctcattt cgcctatctg gatatgattg atgtggttaa tgatttgaaa gatgcaggct
     6661 ataattttga catatcgtgg tttgatccat tcttcgaatt cagatatccg catcacggta
     6721 atgtgactat cgataatatt caattagaaa ttcgattggg aatagaacct tggcacgttt
     6781 tgggcgaaga actctctagc tctggaactt ctagatttgt tgattcctct ttagaacgtt
     6841 tgcaagtaaa ggtttctgga attgttcctg accgtcatat tttattgtgc aaaggctgtc
     6901 gaattccttt aagaagtact ggaactaaag gtgaatatgt ggcaggaatc cgatataaat
     6961 cttggaatcc accttctgca ttgcatccaa atattggcat tgatgtacct cttgtttttg
     7021 acttagttga tacttggaat aataaagtaa ttggtggttg cacctatttt gtaacgcatc
     7081 ccggcggaag aagttttgat actttcccta taaatagttt tgaagcagaa tccagaaaaa
     7141 taagtcgttt ctgggatttt ggccatacgc cttcttcaac agttgaagaa actgaaaatg
     7201 agaaagaaag agaaactatt gtcaacagca atgccacttc tcgattttta gtagaatcaa
     7261 aatcagaact aagacctgat acaccgatag aactaataaa tcctgaatat ccttatactt
     7321 tagatttaag acattattgg aaagccaaag aataatattt aattaagcca attgtattgt
     7381 ggtttgtttt aaaactatta ttttgcaaac caaaatacat gtaaatcatt caatattaac
     7441 aaatgattca agatatttca ttgggattac tcaacacata taaagaaagc atcaattctt
     7501 atgacgaagt tattgaccag aatggccaaa taaagcctta ttggaagggg ctttttgata
     7561 ctttagaatc catcggcatc gaggaactta atttgcgcaa tgaagaaatc ataaaaaaat
     7621 taaaggaaaa cggagttacc tacaatattt atgattccaa caaaaattcg aatcgagcat
     7681 ggaaattaga tcctattccg tttctaattc acgaatcgga atggaaaacg attgaaaaag
     7741 ggttaaaaca aagagcgcga ttgctagatt tgattttgaa agacctttat ggccctcaaa
     7801 cattaattaa aaacgccatt atacctgccg aattagtgtt tgataattca ggttttcttt
     7861 tgccttgttt tgacattaga caaaaacaca acaaacaatt attgaattat gctgttgatt
     7921 tggctcgtgg tcccgacgga aaaatgtggc ttctagacaa ccgaacccaa gcgccttcgg
     7981 gtgcaggtta tgctctcgaa aaccgaattg taatgagtaa ggtaattcct gaactcaaca
     8041 aaaaaacgta tagaaaaaga ctttcaccct acttcagtca aatgcagcaa accgttgaca
     8101 cactagggaa taattctaat gaaaatccaa atgtagtttt cttgactcca ggtcccggaa
     8161 acgaaaccta ttttgaacac gtttaccttt cctcctattt aggatatacc ctagttcaag
     8221 gaaatgactt attggttcgg gatggttttg tttggcttaa atctattgac caactcgaac
     8281 gggttgatgt aattatcaaa cgattggacg acacttggtg tgacccatta gaattacgca
     8341 aagactcttt actaggaatt cctggtttgc tacaagtgat tcggttaggc aatgtatcgg
     8401 tggtgaatcc tcctggaact agtattttag agaattatgg tttaatggct tttatgcaaa
     8461 atgcctgtaa atttcttctc aaagaacctt tattgatgaa ttcagtggct acttggtggt
     8521 gtggtcaagc caaagaattg aattttgttc taaccaattt gcccaaatta atcattaaaa
     8581 aaaccaatag gaaacaaggt tttagatcca t
//
LOCUS       DAOSNN010000096         8571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8196_length_8571_cov_4.346876, whole genome shotgun sequence.
ACCESSION   DAOSNN010000096 DAOSNN010000000
VERSION     DAOSNN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8571
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8196_length_8571_cov_4.346876"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="PLQ70_06325"
     CDS             complement(<1..98)
                     /locus_tag="PLQ70_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34269.1"
                     /translation="MKKFDIYEDPHRRYNPLIKEWVLVSPHRSNRP"
     gene            complement(103..1269)
                     /gene="galK"
                     /locus_tag="PLQ70_06330"
     CDS             complement(103..1269)
                     /gene="galK"
                     /locus_tag="PLQ70_06330"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HQE34270.1"
                     /translation="MNTALIAKTIQSFQKYFGQEPEKVILSPGRINIIGEHIDYNDGF
                     VLPAAIDKIICFAFEKSKTNTSRIIALDLNEEFSVDFSEKISLSDVTWTNYIRGVVNQ
                     LQLNGFQFEGLNCVFSSNIPTGSGLSSSAALECGFIKGVKELYQLDIKPIDIALMGQK
                     AEHWVGINCGIMDQFSSVLGLENKVIKIDCKTLEYEYHNANFGDYSLILLDSNVKHSL
                     VTSAYNKRRQDCENGIALVKKNFPEVKSFRDCTEEQLLALKDQLGEEVYTRCHFVINE
                     IARVAKACEALDNGNIEALGQLLFETHHGLSVEYEVSCEELDFLVDFAKSEPAIIGSR
                     LMGGGFGGCTINLVKKGQEEVIKDKIKKLYYDKFQIELKNYDVKIGNGTTLYTL"
     gene            complement(1459..2742)
                     /gene="murF"
                     /locus_tag="PLQ70_06335"
     CDS             complement(1459..2742)
                     /gene="murF"
                     /locus_tag="PLQ70_06335"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQE34271.1"
                     /translation="MDIKYIHSLFLKCNSVSIDTRKIESNSLFVAIKGDNFDANTFAK
                     EALGKGASYVLIDNENYCIDERTILVENSLIALQELAKFHRQYLKLPIIALTGSNGKT
                     TTKELINVVLSKKFKTKATVGNLNNHIGVPLTVLSFTSETQIGIVEMGANHQKEIEFL
                     CEIATPDFGYITNFGKAHLEGFGGVEGVIKGKSEMYHYLSSNGKLAFINLEDKIQVEK
                     SKTLKSYSFGINKENANVNISAIAANPFVEIQYSNHIIQTHLIGLYNANNINAALTIG
                     KYFGVEEIAIKEALESYIPENNRSQLITKGTNQIILDAYNANPSSMAVAIENFLQLDN
                     SNKIMILGDMFELGQESQEEHKVIVNSLLDTQNVTLYFVGKEFYAHKTDKNDFYFFET
                     FDAFSEYLQTTKMENRSILIKGSRGMALERTLNFL"
     gene            complement(2809..4503)
                     /gene="gldJ"
                     /locus_tag="PLQ70_06340"
     CDS             complement(2809..4503)
                     /gene="gldJ"
                     /locus_tag="PLQ70_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HQE34272.1"
                     /translation="MKVNKIMALRLMLSMILIVGFASCSKKSSSKNTSQGTGWNVDSK
                     KGTSKKQEAGPGLVFVEGGTFTMGKVEDDVMHDWNNTPVQQHVQSFYMDETEVTNAMY
                     LEYLDWTKKVFPPTEESYKDIYEGAVPDTLVWRNRLGYNETMTNNYLRHPSYAKYPVV
                     GVNWVQAVEFSKWRTDRVNEALLEKNGYLKKGAKTNDVTADANFNTETYLNSPTSVYG
                     GNQDIVLPGKYANKKGGVKQPKAPKNGKVVPPTNIYAQRSSGIVLPEYRLPTEAEWEY
                     AAAADVGQREYNIYKGQKKYPWSGSYTRSGKRQSKGDQLANFKQGNGDYGGIAGWSDD
                     GADITNEVKKYPANDFGLYDMAGNVAEWVMDVYRPIVDNEANDFNYFRGNQYTKNKIG
                     KDGKIVIIKSDSIQYTTLSNGKKVATALPGQIAQVPVDENETYLRQNFDKSDNTNYRD
                     GDKQSTRYFNFGDSESDAKKEKDDRKMYNSPNHTVSVDSLGKMVRKYDKNNKRTTLIN
                     DNVRVYKGGSWRDRAYWLDPAQRRYFPQDMATDYIGFRCAMSRVGAKSDKRKSARN"
     gene            4714..8541
                     /gene="porU"
                     /locus_tag="PLQ70_06345"
     CDS             4714..8541
                     /gene="porU"
                     /locus_tag="PLQ70_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HQE34273.1"
                     /translation="MRKTLLVYLILIPFVSFAQIKGNITIEWLEKKEMFVGNSKIIIP
                     KFIGNSYHYDPSKKSLFFILSLSESNYSNENNIQLSNIVYESLSASQLGDLDPENITK
                     NPSAALKINKSRDLTQGFLVLSPIIKDEFGYKRIKSFSYSISGNNSSRIAQTTKTSAL
                     TNSILSSGDWYQFYVEKSGVYKISKNFLLQLGLNVSGIDPKKIKIYGNGGRMLPLSNA
                     IFYPTDLTENAIQVIGESDGVFNSEDYILFYAEGVDTWNEESQTNVNLYDTKSFYYVT
                     VQGNEGKRISNMNQPTGNSTLSLNTFDDHQFHELDLVNIGKLGRQWFGEVFDVNQDQE
                     FSFNFPNIDTATPIKMSVNTASVAYTNTSFKVSANGQDLGTMSFPPLVLYSGIQFNNN
                     SLSNISFPSSESVKIKLSYNNNAVPGSKGYLDKIMLTAKRKLQGYGKQFQFQYDLSKS
                     SLGIVNYDISNANGISQIWDVTDLYNVTKIENTNQASISFKANLGEIRKYVAIDALDY
                     YTPLKTSKTKIANQNLKGTLFKNAQGQIQDIDYVIITPAFLASQAEKLANFHRSYSKL
                     NVKVIQLESIYQEFSSGKQDIAAIRNCIKYIYDNASTSEKRIKYVNLFGDASYDYKNR
                     TSNNTNIVPIYHALNSNTTGESSYASDDFYGLMDASEGNIGSFFGGIDIAVGRMLNND
                     ASQAEEMVNKVIEYHDVKSYGSWRNNFVMIADDSDKSSDASLQYRQNNLADKITFEKP
                     FLNANKILLDSYTQEASAGGSRYTKARTDLFSAFEKGALVFNYLGHGGEDGLASERLW
                     EKSDGQNLNNQYKYPLFITITCDFSRFDNPSRPTAGEYTYWNPKGGAISMITTVREIG
                     QFSAENFNDVLAQNLYSYGSNQYTSIADALRISKNSNPNSSTNVVFYIGDPAIMLAIA
                     KPKIRLTKVNDVPVSQPIDDFKSLAKMKISGEITDENNIPLTNYNGELFTAIFDKFIT
                     RSTLNNDGNSPVIPFTTLGETIFRGNASVANGQFEFSFIVPRDIRIPLNNGKISFYAK
                     KNQQFENQTGYDTSIKIGGINENAVADNISPRVKLYMNDETFVSGGTTNESPFLLANL
                     EDESGINTASGIGHDIIAILDGDVDNPYILNDYYQTNLNDFTKGTLRFPLRNLAVGLH
                     TITFKAWDVYNNPITTEIQFVVAGNDSITLTHVLNYPNPFVNYTEFWFSHNKPFEPLE
                     VQIQVMTITGKVVWTRNQIVTNEGFLSKDITWDGKDDFGNKIGKGVYVYKLTVKSNIT
                     NTKTEKFEKLVIL"
BASE COUNT         2878 a         1578 c         1242 g         2873 t
ORIGIN      
        1 caaggtctat tagatctatg tggcgaaacc aacacccatt ctttgattaa tggattgtat
       61 cttctgtgtg gatcttcgta tatatcaaat tttttcatga ctttataagg tataaagtgt
      121 tgttccgttt cctattttaa catcgtaatt ttttaattct atctggaatt tatcgtaata
      181 taatttcttg attttatctt tgattacttc ttcctgacct ttcttaacca aattaatggt
      241 acaaccacca aaaccacctc ccattaaacg ggaaccaatt atagcaggtt cactcttagc
      301 aaaatcaacc agaaaatcca attcttcaca gctcacttca tattctacag acaatccgtg
      361 atgtgtttca aaaagaagtt gacctaaagc ctcaatattt ccgttatcta acgcttcgca
      421 tgctttggca actctagcaa tttcattgat tacaaaatga catctggtgt aaacttcttc
      481 tccaagttgg tccttcaaag ctagaagttg ctcttcagta caatctctaa aactttttac
      541 ttctgggaaa tttttcttca ccaaagcaat tccgttttcg caatcttgtc ttcgcttgtt
      601 ataggctgat gtaaccaaag aatgtttgac attgctatct aataaaatca atgaataatc
      661 cccaaaattg gcattgtgat attcatattc taatgtttta caatcaattt taattacttt
      721 gttttccaat cctaaaacac tggaaaactg atccataatt ccacaattta tccctaccca
      781 atgttctgct ttttgcccca tcaaagcaat atcaattggc tttatatcca gttgatacaa
      841 ttctttgacc ccttttatga aaccacattc taaagctgca gatgaagaaa gtccagaccc
      901 agttgggata ttgctactaa aaacacaatt aagaccttca aattgaaagc cattcaattg
      961 caattggttg acaactccac gaatgtaatt agtccaagtg acatcactca aactaatttt
     1021 ttctgagaaa tcaacactaa attcttcgtt taaatctaaa gcgattattc gggaagtatt
     1081 ggttttgctc ttttcgaatg caaaacatat tattttatct atggcagcag gcaacacaaa
     1141 tccatcgtta taatcgatgt gctcgcctat tatattgatt ctacctggag ataaaattac
     1201 tttttctggt tcttgaccaa aatatttttg aaatgattga atggtttttg caattaacgc
     1261 tgtattcatg atattaattt ttcacttgaa ttatactcac aaaaataaac ttttttatag
     1321 ctaatgccta accagtacct accagtttat ttatttttta caaccacttc taacttttaa
     1381 aatagcactc ttaaaaacaa aaaacgccgt tataaaaata caacagcgtt ttagaaaaaa
     1441 atttaatatt cttttttatt acaaaaaatt caatgttcgc tctaaagcca ttcctctaga
     1501 accttttata agaatggatc tgttttccat ttttgtagtt tgtaaatatt ctgaaaaagc
     1561 gtcaaaagtt tcaaaaaagt aaaaatcatt tttatctgtt ttatgggcat aaaactcttt
     1621 tccaacaaag tataaagtaa cattttgggt gtctaaaagc gaattaacaa taactttgtg
     1681 ctcttcttga ctttcttgtc ccaactcaaa catatcgccc aaaatcataa tcttattcga
     1741 attatctaat tgcaaaaaat tctcgattgc aacagccata ctacttggat tggcattata
     1801 cgcatccaga ataatctgat tagttccttt ggtaatcaac tgagagcgat tattttcggg
     1861 aatataactt tccaaagctt ccttaatagc aatttcttca acaccaaagt atttaccgat
     1921 tgttaaagct gcattgatat tattggcatt gtacaaacca atcaaatggg tttgaataat
     1981 atggtttgaa tattgaattt ctacaaaagg atttgcagca atagcactaa tatttacatt
     2041 cgcattttcc ttatttatac caaaagaata cgattttaat gtctttgatt tttcgacttg
     2101 gattttatct tctaaattaa tgaaagccaa tttgccgttg cttgaaagat agtgatacat
     2161 ttcactttta cctttaataa ctccttcaac accgccaaaa ccttctaaat gtgcttttcc
     2221 aaaattagta atatacccaa aatcgggtgt tgcaatttcg catagaaatt caatttcttt
     2281 ttgatgattg gcgcccattt ctacaattcc aatttgggtt tcagaagtaa acgacaatac
     2341 tgtcaacggc actccaatat gattgtttaa attgcctacg gttgctttgg ttttgaactt
     2401 tttagaaaga acaacgttta ttaattcctt tgttgtggtt tttccgttgc ttcctgttag
     2461 cgcaattata ggtaatttca aatattgacg gtgaaatttt gctaattctt gcaaagcaat
     2521 taagctgttt tcaaccaaaa tagttctttc atcaatacaa taattttcat tatcaattaa
     2581 cacataggaa gcaccttttc cgagtgcttc tttggcaaaa gtattggcat caaaattatc
     2641 gcctttgata gcaacaaata aagaattcga ttcaatttta cgagtatcaa tcgaaaccga
     2701 attgcatttt aaaaataaac tatgaatgta ttttatatcc atcctaaacc gattttaaaa
     2761 aataaaagcc ctaatcaaag ggcttttact actattatat ataaaaaatt aatttctggc
     2821 agattttctt ttgtctgatt ttgcaccaac tctagacatt gcgcatctaa atccgatata
     2881 atctgttgcc atatcttgtg gaaaatatct tctttgtgca ggatctaacc aataagctct
     2941 atctctccaa gaacctcctt tataaactct tacattatcg tttattagag tagttctttt
     3001 attgttttta tcgtattttc taaccatttt accaagacta tcaacgctca cagtatggtt
     3061 aggagaatta tacatttttc tatcgtcttt ttctttctta gcatcagact ctgaatctcc
     3121 aaaattaaaa tatcttgtcg attgtttatc accatctcta taattggtat tgtcactctt
     3181 gtcaaaattt tgcctcaaat aggtttcgtt ttcatcaact ggaacttgag ctatttgacc
     3241 tggtaaagca gttgctactt ttttaccatt gcttaaagtg gtatattgaa tactatcgga
     3301 tttaatgata acaatttttc cgtctttacc aattttattt ttggtgtatt ggtttcctct
     3361 aaaataatta aagtcattag cttcgttatc tacaattggt ctgtaaacgt ccataaccca
     3421 ttcggctacg tttcctgcca tatcatataa accaaaatca ttggctggat attttttaac
     3481 ttcatttgta atatcagcac catcatcaga ccatcctgct ataccaccat aatctccatt
     3541 tccttgtttg aaattggcca attgatcgcc tttactttgt ctttttcctg atcgagtata
     3601 acttccagac caaggatatt ttttctgacc tttgtaaata ttatattctc tttgtcccac
     3661 atcggcagca gctgcatatt cccactcagc ttcagtaggc aatctgtatt ctggaagcac
     3721 aattcctgat gaacgttgtg cataaatatt tgttggcgga acaactttcc cgttttttgg
     3781 tgcttttggt tgctttacgc ctcctttttt gttagcatat tttccaggta aaacaatatc
     3841 ttgattcccg ccataaaccg atgttggtga atttaaatag gtttcggtat taaaattggc
     3901 atctgctgtt acatcatttg tcttagctcc ttttttaaga tagccatttt tttctaacaa
     3961 ggcttcgttt acacgatctg ttctccattt actaaattca acggcttgaa cccaatttac
     4021 tcctactaca ggatatttag catatgaagg atgtcttaaa taattattag tcattgtttc
     4081 gttataaccc aatctatttc tccaaaccaa tgtgtctgga acagcacctt catatatgtc
     4141 tttataactc tcttctgtag gaggaaatac ttttttggtc caatccaaat attctaaata
     4201 cattgcattg gtaacttctg tttcatccat ataaaaagac tgcacatgct gttgaactgg
     4261 agtattattc caatcatgca ttacatcatc ttctacttta cccattgtaa aggttcctcc
     4321 ttctacaaat accaatccag ggccagcttc ttgctttttt gaagtgcctt ttttactatc
     4381 tacattccat cctgtgcctt gtgaagtatt ttttgagcta gattttttac tgcaactagc
     4441 aaaaccaaca atcaaaatca ttgataacat caaacgtaag gccataattt tgtttacttt
     4501 catactcttt agtaggtgat aatttctgag gtgcaatata ataattaacc attaaaccgc
     4561 aacaacattt tttattttat aaattgattc ttgaattctt aggtaaaact cacaatccaa
     4621 acgcaaattt atactattta ttattagaat tacatgaaat gatatatttt tactcgccgt
     4681 aatattttac aaattattgc gttaaacaaa acaatgagaa aaacactttt ggtttattta
     4741 atcctaattc cttttgtttc ttttgcgcaa ataaaaggaa acataaccat tgaatggctt
     4801 gaaaaaaaag aaatgtttgt aggcaactct aaaattatta taccaaaatt tattggaaat
     4861 agttatcatt acgacccttc aaaaaaatca ctttttttta ttctaagcct atctgaatca
     4921 aactattcaa acgaaaacaa cattcaacta tcaaatattg tttatgagtc tttatctgct
     4981 tctcaacttg gagatttaga ccctgaaaac attacaaaaa acccatcagc cgcattaaaa
     5041 atcaacaaat ctagagacct tacacaaggt tttttagttt tatcccctat tatcaaagat
     5101 gaatttgggt acaaacgaat taaatctttc tcttattcta taagtggcaa taactcttca
     5161 agaattgcac aaactacaaa aacaagtgcc ttaactaact ctatattatc ttctggagat
     5221 tggtaccaat tttatgttga aaaatcgggg gtttataaaa tttcaaagaa ctttttactg
     5281 caattaggac ttaatgtaag cggaatcgac cctaaaaaaa taaaaattta cggaaatggc
     5341 ggcagaatgc tacccctatc gaatgcgata ttttatccaa cggatttaac cgaaaatgct
     5401 attcaagtaa ttggagaaag tgatggcgtt tttaactctg aagattacat tttattttat
     5461 gccgagggag tcgatacttg gaacgaagaa agtcaaacca atgttaactt atatgacaca
     5521 aaatcctttt attatgtaac tgttcaagga aatgaaggaa aaagaatctc aaatatgaat
     5581 caaccaacag gaaatagcac gttgagtttg aacacttttg acgaccatca atttcacgaa
     5641 ttagacctag taaacattgg aaaacttggc agacaatggt ttggagaagt ttttgatgtc
     5701 aatcaggacc aagaattttc gtttaatttt ccaaatattg atacagcaac gcccataaaa
     5761 atgagtgtaa acacagcttc tgtagcatac accaatacat ctttcaaggt atcggcaaac
     5821 gggcaagacc taggaacaat gtcctttcct ccgcttgttc tatattcagg aattcaattt
     5881 aataacaact cattatcaaa tatttcattc ccaagttcgg aaagcgtaaa aattaaatta
     5941 tcctacaata ataatgctgt tccaggttca aaaggatatt tagacaaaat tatgcttacc
     6001 gccaagagga aactgcaagg ctacggaaaa caatttcaat ttcaatacga tttatcgaag
     6061 tcgagtttag gaatagtaaa ttatgacatt tcgaatgcaa atggaatttc acaaatttgg
     6121 gatgttacag acctatataa tgtaaccaag attgaaaaca caaatcaagc ttcaatttct
     6181 ttcaaagcaa atttaggtga gatccgaaaa tatgtagcca ttgatgccct ggattattac
     6241 acccctttaa aaactagcaa aaccaaaatt gcgaatcaaa acttaaaagg aactcttttc
     6301 aagaatgctc aaggtcagat tcaagatatt gattatgtga taataactcc agcattctta
     6361 gcatcacaag ccgaaaaatt ggccaatttt catcgttctt attctaaatt gaatgtcaaa
     6421 gtaatacaac tggaatcaat ttatcaagaa ttctcctcgg gcaagcaaga tattgccgct
     6481 attagaaatt gtattaaata catttatgac aatgcttcga catctgaaaa aagaataaaa
     6541 tatgtcaatc tttttggaga cgcttcctat gattataaaa accgaacttc aaacaatact
     6601 aatattgtac ctatatatca tgcattaaat agtaatacta ccggagaatc ctcctatgca
     6661 tcggatgatt tttatggttt aatggatgct agcgaaggaa atattggttc tttctttggc
     6721 ggaatagaca ttgctgttgg aagaatgctt aataatgacg cttctcaagc agaagaaatg
     6781 gtaaacaaag taattgaata ccacgatgta aaatcatacg gaagttggag aaataatttt
     6841 gttatgatag ccgatgattc cgataaatcc tcagatgcta gcttgcaata cagacaaaac
     6901 aatttggccg ataaaatcac ttttgaaaaa ccttttctta atgctaacaa aatattacta
     6961 gattcttata cacaagaggc atcagctgga ggatccagat acacaaaagc tcgtactgat
     7021 ttgtttagcg cattcgaaaa aggggctttg gtttttaatt atttaggcca cggtggcgaa
     7081 gatggtttgg ctagcgaacg actttgggaa aaatcagacg gacaaaactt aaacaaccaa
     7141 tacaaatacc cattattcat aaccataact tgcgattttt cgagatttga taatccttca
     7201 cgaccaacag caggcgaata tacttattgg aatccaaagg gaggagcaat atcaatgatc
     7261 actactgtcc gagaaatagg acagtttagt gccgaaaact tcaatgatgt tttggctcaa
     7321 aatttatatt cctacggctc taaccaatac acttcgatag ccgatgcatt acgaatttca
     7381 aaaaacagca acccaaattc atctaccaat gtagtgtttt acattggcga cccagcaata
     7441 atgcttgcca ttgctaaacc aaaaataaga cttacaaaag taaatgacgt tcctgtttca
     7501 caacccattg atgatttcaa gtcattagca aaaatgaaaa tatcgggtga aataaccgat
     7561 gaaaataata ttccactaac caattataat ggagaactat tcacagcaat atttgataaa
     7621 ttcataacta gatccacctt aaacaatgat ggaaacagcc ctgtaattcc atttacaaca
     7681 cttggagaaa ccatctttag agggaatgct tcggtggcaa acggtcaatt tgaatttagt
     7741 tttattgtgc caagagacat ccgaatccca ttaaacaatg gaaaaattag tttctatgct
     7801 aaaaaaaatc aacaattcga aaatcaaaca ggttatgaca ccagcataaa aataggtgga
     7861 ataaatgaaa atgcggttgc agacaatatt agtccaagag tgaagttata tatgaacgat
     7921 gaaacttttg tttcaggtgg aacaacaaat gaatctccgt tcttattagc caatcttgaa
     7981 gacgaaagtg gcatcaatac tgctagcgga attggtcacg atataattgc cattttagac
     8041 ggagatgttg acaatccgta tatcttaaac gattactatc aaaccaattt aaatgatttc
     8101 acaaaaggaa ctttgcggtt tccactgcga aatttagccg taggcttaca caccataact
     8161 ttcaaggctt gggatgttta caataatcca attactaccg agattcagtt tgtggtagca
     8221 ggaaacgatt cgataacctt aactcacgtg ttaaattatc ctaatccttt tgtaaattat
     8281 actgagtttt ggttttcaca caacaaacct tttgaacctt tagaagttca aattcaggta
     8341 atgactatta caggaaaagt ggtttggaca cgaaaccaaa tcgtaactaa tgaaggattt
     8401 ctatcaaaag acatcacttg ggacggcaaa gatgactttg gaaacaaaat aggaaaagga
     8461 gtttacgtgt ataaactcac cgttaaatcg aacataacaa acacgaagac cgaaaaattt
     8521 gaaaaacttg taatacttta attaaatact atatttgtcc aattattatt t
//
LOCUS       DAOSNN010000097         8476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8315_length_8476_cov_6.220045, whole genome shotgun sequence.
ACCESSION   DAOSNN010000097 DAOSNN010000000
VERSION     DAOSNN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8315_length_8476_cov_6.220045"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..743)
                     /locus_tag="PLQ70_06350"
     CDS             complement(198..743)
                     /locus_tag="PLQ70_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HQE34274.1"
                     /translation="MNLQTLEKLKFPIGEFHKTDSISKTDIENWIATIEAFPSKIKSL
                     TQSLSIEELNWIYRPKGWSIKQVVHHCADSHMNSLIRFKLALTEDVPDIKPYEEQLWA
                     ELADGNSNDISPSLQIIEGVHARWVLLLKSFGTKELKKQFFHPGNMKIVSLEENIGVY
                     AWHCNHHLAHIEQALLYKGQF"
     gene            complement(776..2080)
                     /locus_tag="PLQ70_06355"
     CDS             complement(776..2080)
                     /locus_tag="PLQ70_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HQE34275.1"
                     /translation="MLHKILKFTDLHSGEEDKKKVLENVKSNISFRGSNLWILACAIV
                     VASIGLNVNSTAVIIGAMLISPLMGPIIGAGFGLGMYDSELVKKALKNLLIATIVSLV
                     VSTTYFYLSPFKETQSELLARTSPNIYDLLIAFFGGLVGVIAITRVEKGNPIPGVAIA
                     TALMPPLCTAGYGLAIGNLKFFLGALFLYTINCVFICISTFLIVKYLKYPAVKQVDVK
                     RQKQIKYGISALTLALMLPSIFFAYTLFDEKKTSQKINLFIENEFTNKGYTILYKNIN
                     LNTSPKKIELAFLRQKFNTNEIKSLNKSLNNYQIENTELHIKQDTIDIKKYIIDQIQS
                     GKTNLDTKDITINQLKNEIASNAYNNKELLEEIKIIFPQVENISISNHSFIKNKDSAY
                     VRPVLIYDSKTNLTDESKQKLKLWLQKRLSKDKIEIYKQETN"
     gene            2313..2645
                     /gene="ssb"
                     /locus_tag="PLQ70_06360"
     CDS             2313..2645
                     /gene="ssb"
                     /locus_tag="PLQ70_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQE34276.1"
                     /translation="MNAMKNSVQLIGNVGNDPEIKIFDGGKKLVNLTIATNDSYKNDK
                     GEKVETTEWHRVAAWGSTADIIEKYVTKGLQIAVEGKLTHRSYDDKNGEKRYITEVVV
                     NEVLLLGK"
     gene            complement(3834..4352)
                     /locus_tag="PLQ70_06365"
     CDS             complement(3834..4352)
                     /locus_tag="PLQ70_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013550646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HQE34277.1"
                     /translation="MLSKNIEVALNKQIRIEAESSQIYLSMACWAEVNGLEGISQFMY
                     AQSDEERLHMLKLIKYVNERGGHAQITELNAPKTSYETFKGMFEELYKHEIFVSNSIN
                     ELVHITFQEKDYATHNFLQWYVAEQIEEEATAKTILDKINLIGDDKGGLYLFDRDIQQ
                     IAVTAGTVAAPK"
     gene            complement(4508..5137)
                     /locus_tag="PLQ70_06370"
     CDS             complement(4508..5137)
                     /locus_tag="PLQ70_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HQE34278.1"
                     /translation="MKTLLDIENWPRKEHFQFFKQFEEPFFGLTLEIDCTKAYATAKE
                     LESSFFVYYLHKTLVAVNTIECFRYRIHNDAIYVYDRIDVSATILREDNTFGFSLIEY
                     SPNFEVFTKNAFQEMERIQNTSGLLTREFPNDNLIHFSAVPWVNFTSLSHARSYTFPD
                     SCPKISFGKMMIDTNGKKTMSMSIHAHHGLMDGFHIGQFVDCFQKLMNQ"
     gene            complement(5296..6672)
                     /locus_tag="PLQ70_06375"
     CDS             complement(5296..6672)
                     /locus_tag="PLQ70_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34279.1"
                     /translation="MKKLLLFLLLTYKSTLLGQTILTSFPLDLTHSKENNQILTVENI
                     RTHNVFVFATDKKNITILKYNSALFLTDQYSLQVNQNCKSLIGYSFGEDGNPTLYWSS
                     EGFNTILIVKYYLENKTCEILNFKYSFWDQDVVASFQKNNMFYILSKYKYKQALNVHT
                     FRDKKVEERIFDFSGFKFQNQNTQFLRFNQVIIKNPIEKIEVDDYNPLYKSTKKSKVY
                     MLEDEIILTLDHNSKKTQVFELNLKSHDLNEKTFIQSSLQTPKKISNSFYQDGKLYQI
                     NASESELLLDIKDFNTSQTIKSVKVSENETISFNNSPLVIQKDNRKPIELKKTKKFLE
                     HLFLLDIGLSVFKKNLNTLIAVAGTPKEEEPYKPSALDYEFFGGNVPYIYHTESVYFE
                     SILDSNFEFIHQKEEPHAIDNIFYFLNTNKKITSKSILKFKDFYILGYYDDIAKQYIM
                     RKFTDGFF"
     gene            complement(6836..7522)
                     /locus_tag="PLQ70_06380"
     CDS             complement(6836..7522)
                     /locus_tag="PLQ70_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQE34280.1"
                     /translation="MNLKVIAFDADDTLFVNEPYFQETEEKFCGLMSDYLSHQGISQE
                     LFKIEIDNLKIYGYGIKAYILSMIEAAMKISNNTIPVEVIEKIIEYGKELLEKPIELL
                     DGVEETLQALHGKYKLVVATKGDLLDQRRKLHNSGLGQYFHHIEVMSDKQERDYSDLM
                     KRLEIQPEEFFMIGNSLKSDVLPVLALGGHAVHIPFHTTWAHERIDFKVEHENFYAFE
                     KITNVLKILS"
     gene            complement(7592..>8476)
                     /gene="metG"
                     /locus_tag="PLQ70_06385"
     CDS             complement(7592..>8476)
                     /gene="metG"
                     /locus_tag="PLQ70_06385"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010254883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HQE34281.1"
                     /translation="IFGNFINRVVVLTNKYYGGIIPTPNELSEVDEATLTELKAYPAV
                     ISSSIERYRFREALGELMNVARLGNKYLADEEPWKMVKTDPARVQTQMYVALQIAAAL
                     SSLCEPFLPFTAAKLSRILNIKNPIDWKTVADDSDLIPAGHQIGEAELLFAKIEDEEI
                     QKQIDKLEATKTANTAENKVAEPQKDLIQFEDFAKMDLRVGTILEAEKMPKANKLLIL
                     KVDTGIDARTIVSGIAESFKPEDIIGKRVTVLVNLAPRNLRGVESQGMILMTNNAEGK
                     LVFVNPDADGVANGETIN"
BASE COUNT         2637 a         1328 c         1376 g         3135 t
ORIGIN      
        1 tgtaggcatt gcgacaacca tcgctgcaaa tctttttgtc ttcgcgaccc acaattttgt
       61 ctccacattc taggcaggtt ttgttcatag tttttctttt ttaccgtttt tttttaaacc
      121 attaagagca ttaagaaaat taagttttgt tttttcttat tgagccttaa tgttcttaat
      181 ggtttgacat ttatttctta aaactgacct ttatacaaca aggcttgttc aatatgtgcc
      241 aaatggtggt tgcaatgcca agcataaacc ccaatgtttt cctccaaact aactattttc
      301 atattgccag gatgaaagaa ttgtttcttt aattcttttg ttccaaaaga tttcaaaagt
      361 agcacccaac gagcgtgaac accttcgatg atttgaagtg atggagaaat atcattcgaa
      421 ttgccatcgg cgagttcagc ccaaagttgc tcttcatagg gttttatgtc gggaacatct
      481 tcggttaaag ccagtttaaa tcgaatcaaa ctattcatat gactgtcggc acaatgatgc
      541 acgacttgtt ttatcgacca tccttttggt ctgtaaatcc agttcaattc ttcgatagaa
      601 agactttggg tcagactttt tattttggat ggaaaagctt caatagtggc aatccaattt
      661 tctatatcag tttttgaaat agaatcggtt ttatgaaatt caccaattgg gaattttagt
      721 ttttcgaggg tttgaaggtt catttttctt taattttaga aacaatcctt atctgttaat
      781 tggtttcttg tttataaatt tcaattttat ctttggagag tcttttttgc aaccacaact
      841 ttagtttttg tttggattca tcagttaaat tggtcttaga atcgtaaatt aaaacggggc
      901 gaacataagc actgtcttta tttttaataa agctatgatt ggatatcgag atgttttcga
      961 cttgggggaa aatgattttt atttcttcca gtaattcttt attgttgtat gcattggatg
     1021 caatttcatt tttcaattga ttgattgtga tgtctttggt gtccaaattt gtttttccag
     1081 attggatttg gtcaataata tattttttta tgtctattgt atcttgttta atatgcaatt
     1141 ctgtattctc aatttgataa ttgtttaggg atttgttaag tgacttaatt tcgttagtat
     1201 taaatttttg tcttaaaaaa gcaagttcaa tttttttagg actggtattt agatttatgt
     1261 ttttatacaa aatagtataa cctttatttg taaattcatt ttcaataaaa agattgattt
     1321 tttgactcgt ttttttctca tcaaataggg tataagcaaa aaagatgctg ggtaacatta
     1381 aggctagagt caaggcagaa atgccatatt ttatttgttt ttgacgtttg acatcgactt
     1441 gttttactgc tggatatttt aaatatttaa cgattagaaa agttgaaatg caaataaaaa
     1501 cacagtttat ggtgtataaa aacagagcgc ccaaaaagaa ttttaggttg ccaatggcca
     1561 atccatatcc agcagtacac aaaggtggca ttaatgctgt tgcgatggcg acgccaggaa
     1621 ttggattccc tttttcgacc ctggtaatgg caattactcc tacaagtcct ccaaaaaagg
     1681 caatcaataa atcataaata ttaggcgaag ttctggctaa taattccgat tgagtttctt
     1741 tgaaaggact caaataaaaa taggttgtcg aaacgactaa actaacgatt gtggctatta
     1801 aaagattttt taatgctttt tttaccaatt cagaatcata cattccgagt ccaaatccag
     1861 caccaataat tggtcccatt aagggtgaaa tgagcattgc cccaataatc acagctgttg
     1921 aatttacatt caatccgatc gaagcaacca caatagcgca ggccaaaatc cacaagtttg
     1981 aaccccgaaa tgagatatta gattttacat tttctaaaac ttttttcttg tcttcttctc
     2041 cagaatgaag gtcggtaaat tttaatattt tatgaagcat ctctttttta tgatttttac
     2101 ttgaaatttg ttaacgtata aaaaatcaat aattctttat aaatcaatca aatatagtaa
     2161 ataatttcca tttacaaacg gttacaaaca gttacaaccg aaagtaagta gttatcaacc
     2221 gaataatttt tgaaagccgt cgcatctttg ccctgttgat ttgaacgagt cattcaacaa
     2281 actatatata aacaatttaa attttacacg ccatgaatgc aatgaaaaac tcagtacagt
     2341 taatcggaaa cgtaggtaat gatccagaaa tcaaaatttt tgatggagga aaaaaattag
     2401 tcaatttaac catcgccaca aatgatagtt ataaaaacga caaaggagaa aaagtggaaa
     2461 caaccgaatg gcacagagtg gccgcttggg gatcaacggc cgacatcatc gaaaaatatg
     2521 tgaccaaagg tcttcaaatt gctgttgaag gcaaattaac ccacagaagt tatgacgata
     2581 aaaacggaga aaaacgatac attacggaag tcgttgtgaa tgaagtgttg cttttgggca
     2641 agtagtttaa tttaatttta attaatgcgg caaagtaata gctttgtcgc atttttttag
     2701 ggttaatttg aattacttag ttaagaactt taatttaatt ttttttaagg attatgtttc
     2761 tatatcttgc aactgtagat ggtatataaa atatggtttg tcgataaaaa aatattttgt
     2821 aggtttaata gcgtaatcta acatggtgat tttatataaa ttgtagatag ccgcattttt
     2881 ttacacttaa tttaagaatt atcttgttaa ttattaaaat ttaatgctgg tcgtcggaaa
     2941 taatgtgttt tttaattaaa aaaatcgtat atactttact tttgtgtaat caaatcgttt
     3001 ggtataattt attataaaaa aaaccaatgt tagagcattg gttttaattg aatagattat
     3061 ttttagtaat cttttatttt cgcgatatga aaatactatt tttaatttac tgttctttgg
     3121 ttgttttcca agtaacagaa ctattaagac aaattagaaa attaattttt gtaattaaat
     3181 cttaacttaa agtcccatta gaaatattaa attttctaat gggttttatt tttataattc
     3241 aaaggtattt aaacaaatca aattaattat aacaaagccg ttgaaataca tattttttat
     3301 tttttttgag taattattct taccttttat aagataaatt ttaattaatc attccttaat
     3361 ttttagtaaa accacaaact ttagatgagc ttaaacgaat taaaagtttt ggagaaatta
     3421 ggactgctaa ttatggagat gatattattt ctcttttgaa cgctttgtag aattaactat
     3481 taaaaaaaat gccctctcat tcggagggca tttgtattta ggatactttt ttgaataaat
     3541 cgtgcatagg acagcggtcg caacgtttct tttcgctttt tttatatttt tcgcaacaac
     3601 tagatttgca gttttctgct ttcttgattt tgctaagaag atcttttttc tttttcttct
     3661 ttttctcttt ttccttttct ttcttgctca ccttgtgaag ggttaaaaat ccaacacaaa
     3721 gatatgttat ttttatttat tctaaataag atttatcatt ttattttcaa aaaaaatacc
     3781 ttatagaaaa acaatttctt taaggtattt attaaaattt ttgaaaattc aaattatttt
     3841 ggtgcagcta ctgttcctgc agtaacagca atttgttgaa tatctcggtc aaataaataa
     3901 agaccacctt tgtcatctcc aatcaaatta attttatcta aaatagtttt ggcagtggct
     3961 tcttcttcaa tttgttcggc aacataccat tgtaagaaat tatgggttgc ataatctttt
     4021 tcttgaaaag tgatgtgaac caattcatta attgaattcg aaacaaaaat ttcatgtttg
     4081 tataattctt caaacattcc tttaaaggtt tcataagaag tttttggagc attgagttct
     4141 gtaatttgag cgtgacctcc tctttcgtta acatatttta ttaacttgag catatgtaaa
     4201 cgctcctcat cagactgtgc atacataaat tgtgagatac cttctaatcc atttacttct
     4261 gcccaacaag ccatagataa ataaatttgc gaagactcgg cttcaatgcg aatttgtttg
     4321 tttaacgcga cttctatatt ttttgatagc ataattttgt tgttttaagc aaatttaatt
     4381 aaaataattc aaagaataga ttggtttaaa aaagattttt atccttgtaa tttctttaaa
     4441 aggagagtta ttttattcga ataagttaat ttttgaatcc aatttatcaa aaagacagta
     4501 ttctaaatta ttgattcatc aatttctgaa agcaatccac aaattgaccg atgtgaaaac
     4561 catccatcaa tccgtgatga gcgtgaatag acatcgacat tgttttcttc ccattagtgt
     4621 caatcatcat ttttccaaaa gatattttag ggcaactatc cggaaaggta tagcttctag
     4681 cgtgagaaag tgaggtaaaa ttaacccaag gaacagccga aaaatgaatc aaattatcgt
     4741 ttggaaattc acgggtcaaa agtcccgatg tgttttgtat tctttccatt tcctggaatg
     4801 cgttttttgt aaaaacttca aaatttggag agtattcaat taacgaaaaa ccgaaagtgt
     4861 tgtcttcacg cagtatagtt gccgaaacat caatcctgtc gtaaacgtaa atagcatcgt
     4921 tatgtattct ataccgaaag cattcaatag tgtttacggc aaccagggtt ttgtgcaaat
     4981 aataaacaaa aaaggaagat tctaattctt tggcagtggc ataggctttg gtgcaatcta
     5041 tttcaagggt taaaccaaaa aaaggttctt caaattgttt gaaaaattga aaatgttctt
     5101 tcctgggcca gttttctata tctaaaaggg ttttcacagt tgtattattt ttagaatatt
     5161 cattattttt tcgaaggtgt aaatgttttc gtgttgcagc ttgaaatcaa ttctttcgtg
     5221 tgcccaagtg gtatgaaacg gaacctattt ttgtgagttg actatcatca aatcgcttcg
     5281 agtggaagtt tttcctcaaa aaaatccatc tgtaaattta cgcataatat attgttttgc
     5341 aatatcatcg taatagccta agatataaaa atctttaaat tttaatatgc tttttgatgt
     5401 tattttttta tttgtgttta aaaaataaaa aatgttgtca atagcatgag gctcttcttt
     5461 ttgatgtatg aactcaaaat tagaatccaa tatgctttca aaataaacac tttcagtatg
     5521 atagatgtag ggaacgtttc ctccaaaaaa ttcgtaatcc aaagcagatg gcttataagg
     5581 ttcttcctct tttggagttc cagccacagc aatcaaagta tttagatttt ttttaaaaac
     5641 agataaacct atatctaaaa ggaatagatg ttcaagaaat ttttttgttt tttttaattc
     5701 tattggtttt ctgttgtctt tttgaatgac taatggggag ttattaaaag aaattgtttc
     5761 attttcggag actttaacgc tttttatggt ttggctagta ttaaaatctt ttatatcgag
     5821 caaaagttcc gattcactgg cgttaatttg gtaaagcttg ccatcttgat agaatgaatt
     5881 acttattttt ttgggcgttt gaaggcttga ttgaatgaat gttttttcgt ttaaatcgtg
     5941 gcttttcaaa ttaagttcaa atacttgggt cttttttgag ttatgatcca atgttaaaat
     6001 aatttcgtct tccagcatat atactttact ttttttggtg cttttgtaca aaggattata
     6061 atcatccact tcaatttttt caatgggatt ttttataatg acttgattaa atctcaagaa
     6121 ttgggtattt tgattttgaa atttaaaacc agaaaagtca aaaattcttt cttctacctt
     6181 tttatctcta aaagtatgga catttagtgc ttgcttatat ttgtatttgg aaagaatata
     6241 aaacatatta ttcttctgaa atgatgcgac tacatcctga tcccaaaaag aatatttaaa
     6301 atttaaaatt tcacaagttt tgttttccaa ataatatttg acaattaaaa tagtattaaa
     6361 tccttccgaa gaccaataaa gagttgggtt tccatcttcg ccaaaactat atccaatcaa
     6421 tgatttacag ttttgattta cttgtaagct atattgatcg gttagaaata aagcactatt
     6481 gtattttaaa atggtgatgt tttttttatc tgttgcaaaa acaaacacat tgtgtgttcg
     6541 tatattctca acagttagga tttggttgtt ttctttcgaa tgtgtcaaat ctaatggaaa
     6601 agaagtcaat attgtttgac ccaacaatgt tgatttgtat gttagaagca aaaaaagaag
     6661 tagttttttc atactttttc atttactaaa attgtgccaa tttattagtt catttgacac
     6721 gatttaaaat ttggggggaa tagaaaaaaa tttattagaa ttttatttca aactttgact
     6781 tgcacttttt taattaagtc taatgagtga attgttcgaa aaatcctttt tgcatttaac
     6841 ttaaaatctt caagacgttg gttatttttt cgaaagcata aaaattttcg tgttccacct
     6901 tgaaatcgat tctttcgtga gcccaagtcg tgtgaaacgg aatatgaacg gcgtgtccgc
     6961 caagtgccaa aaccggcaaa acatccgatt tcagcgagtt gccaatcata aaaaattcct
     7021 cgggttgaat ttccaaacgt ttcatcaaat ccgaataatc cctttcctgt ttgtccgaca
     7081 tcacttcgat gtggtgaaaa tactggccta atcccgaatt gtgtagtttg cggcgttgat
     7141 ccaacaaatc gcctttggtc gcgacaacga gtttatattt tccgtgcaaa gcttgcaagg
     7201 tttcttccac gccgtccaat aattcgatgg gtttttcgag taattctttg ccgtattcga
     7261 tgattttttc aatgacttca acaggaatgg tattgttcga aattttcatc gccgcctcaa
     7321 tcatcgaaag aatatacgct ttaattccgt agccgtagat tttcaaattg tctatttcaa
     7381 tcttgaaaag ttcctgcgaa atcccttgat gtgacaaata atcactcatc aacccacaaa
     7441 atttttcctc cgtttcctga aaataaggtt cgtttacaaa caaggtgtcg tcagcgtcga
     7501 aagcaattac ttttaggttc atttttattt tttttgccac gaattcacga atttttaaag
     7561 ttttttaaat tcgtgaattg gtggctaatt tttaatttat cgtttcgcca ttcgcaacac
     7621 catcggcatc aggatttacg aaaaccagtt ttccttcggc gttgttggtc atcaaaatca
     7681 ttccctgact ttccacgcct cgaaggtttc ttggagccag atttaccaag actgtcactc
     7741 gttttccaat aatatcttcc ggtttaaaac tctctgcaat tcctgaaaca atcgtgcggg
     7801 cgtcaattcc ggtatcgact ttcaaaatca aaagtttgtt ggctttcggc attttttctg
     7861 cttcgagaat cgttcctacg cgcaaatcca ttttggcaaa atcttcaaac tgaattaagt
     7921 ctttctgagg ttctgcgact ttgttttcgg cagtgttggc agttttggta gcttccaatt
     7981 tgtctatttg tttttggatt tcttcgtctt caattttggc aaaaagcaat tcggcctccc
     8041 caatttggtg tccagcagga attaaatcgg aatcatctgc aaccgttttc cagtcaattg
     8101 gatttttgat gttgaggatt ctggataatt ttgcagccgt gaaaggcagg aaaggttcgc
     8161 aaagtgaact caaagcagct gcaatttgca aagccacata catttgggtt tgcacgcgag
     8221 ccggatcggt ttttaccatt ttccaaggct cttcatctgc taaatatttg tttcctaatc
     8281 gagcaacatt catcaattcg cccaaagctt cacggaatcg gtaacgctct atcgaactcg
     8341 aaattactgc tggatacgct tttaattcgg ttaaagtcgc ttcgtccact tccgaaagtt
     8401 cgtttggagt tggaataatt cctccgtaat atttgttggt caacaccacc acacgattga
     8461 tgaaatttcc aaaaat
//
LOCUS       DAOSNN010000098         8420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8386_length_8420_cov_6.082487, whole genome shotgun sequence.
ACCESSION   DAOSNN010000098 DAOSNN010000000
VERSION     DAOSNN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8386_length_8420_cov_6.082487"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLQ70_06390"
     CDS             complement(<1..601)
                     /locus_tag="PLQ70_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34282.1"
                     /translation="MNLTESISKLNTSNLFLNNGNNGYLNYYELFLLYFNAIPNSTEE
                     ININCEKASANFFDKYENEIKENFYQKEVFENGTKSKLKQVYYILDEMIIIFYMDYNS
                     VQFLFLNQNLSKLQSIIEGIIKCKKRKSKQPQEISLLTNGARGITTTSLKISKPKLKV
                     EDNYNDDFQEIHQTIYKRLSKNNDKGLILLHGKPGTGKTS"
     gene            complement(773..1996)
                     /locus_tag="PLQ70_06395"
     CDS             complement(773..1996)
                     /locus_tag="PLQ70_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQE34283.1"
                     /translation="MAKIKEINNNYLYWDKVKYKKVNGLSAEEIWNTVKLVRSINYKQ
                     IKFSNVVFNYFNTDYIQKSLHEFDMNIGGYMGSQKIIPESDKARYLVSSIMEEAISSS
                     IMEGANTTRKKAKDMLRKDTKPINKSEQMIVNNYTTIKHILKNKNEELTPENLLEIHQ
                     LMCKNTLKNENEVGAFRTSNDIYVVNHSTSEVVHTPPDFVQINKLIQELCQFFNSEKE
                     DFIHPIVKGIIIHFMIGWIHPFTDGNGRTARALFYWYLLKKGYWLTEYLSISKIIQGS
                     KNQYEKAYLYTENDANDLSYFITYHIEVMHKAFESLKNYIQEQQKDNLQIASFIKIPN
                     INERQAQILKTLYDEPECVFSVKEVQNRFSVANYTARVDLNNLVNMGFMEVISVNKVK
                     QNYIRSSKFNELIKN"
     gene            2226..2489
                     /locus_tag="PLQ70_06400"
     CDS             2226..2489
                     /locus_tag="PLQ70_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HQE34284.1"
                     /translation="MDILLYYFLILNGIAFLLMGYDKHLAQAQKQRISERTLLSFAFF
                     GGTIGSGLAMLIFRHKISKKSYLLKFWFVVIVQFLIVWLYLNH"
     gene            complement(2556..3473)
                     /locus_tag="PLQ70_06405"
     CDS             complement(2556..3473)
                     /locus_tag="PLQ70_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQE34285.1"
                     /translation="MGQHHNHEHHHHHQVEGKNLLFSIILNIVITVAQVVGGILSGSL
                     ALLSDALHNFSDVLSLVFSYVAHKLAKREASVNQTFGYKRAELIAAFVNAMTLIIVAL
                     FLVYGAVERFFNPEPIKSGLVIWLSIIGIVANGLSVLLLKNDADKNLNMKSAYLHLFT
                     DMLASVAVLVGGLLMKYFQWFWVDSVLTLAIAIYLLVVGFDLLKDSTKMLMLFTPTHI
                     DINEIIEEVHKIPGTDKLHHIHVWHLNDDELHLEAHLDCSEDIKMSEFNEILHKIEHV
                     LFDKFQINHINIQPEYNREEASKDFIVQD"
     gene            complement(3478..3906)
                     /gene="rpiB"
                     /locus_tag="PLQ70_06410"
     CDS             complement(3478..3906)
                     /gene="rpiB"
                     /locus_tag="PLQ70_06410"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HQE34286.1"
                     /translation="MKISIGNDHAGPEYKKAIVEYLESKGYEVTNYGTDTDASVDYPD
                     FGHPVANDVSEGKADFGIVICGSGNGIAMTVNKHPKVRAGLCWIKEIAYLTRLHNNAN
                     VLSIPARYTSIQQAIEIVETFLTTEFEGGRHQTRVDKISC"
     gene            complement(4551..4784)
                     /locus_tag="PLQ70_06415"
     CDS             complement(4551..4784)
                     /locus_tag="PLQ70_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HQE34287.1"
                     /translation="MDHFQTIASFNFAHEIIVLKSILQNEGIPFLFQNENLISIDPLA
                     SIAYGGIHLKVHKNDFERVQNILDELNNNLKIV"
     gene            4893..7073
                     /gene="rnr"
                     /locus_tag="PLQ70_06420"
     CDS             4893..7073
                     /gene="rnr"
                     /locus_tag="PLQ70_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQE34288.1"
                     /translation="MSKKLRKPVKKEKDFSEKIIKILAQNANKAFNYKQIAAILEKDD
                     TKSRNEIIRDLKILASSKKIIETEPGKYLVKAISQDYYEGTIDMTGRKTAYFICPDFE
                     EDVFIPTNNLNRALDKDKVKVYVYNRRKGRRPEGEVIEVVERAKTDFVGVIDIQKNFA
                     FVSTANPKMYTDIFIPKDKLGEAEQGDVVLVHIEDWPKRADSPFGTVVKVLGKPGEHD
                     TEIHAILAEYGLPAEFPVEVEVYAQKIDTSITEEEINKRRDMRDTLTFTIDPKDAKDF
                     DDALSFKKLDNGNYEIGIHIADVSHYLQEGTILDDEAYQRATSVYLVDRVVPMLPEVL
                     SNFACSLRPQEEKYTFSAIFEISEKAQVVNQWFGRTVIFSDQRFAYEEAQYIIEMKDN
                     IIPAEISITGTSYKVSDEIVAATLKMQDLAKILRRNRMNNGAISFDKVEVKFNLDNEG
                     EPEGVYFKISKDANHLIEEFMLLANRKVAEYVGKQKKTFIYRIHDEPNEDKLIAMQTV
                     IAKFGYKIDFRNKGDISKSLNALMVEVNGKKEQNLIDTLAIRTMSKAKYSTDNIGHYG
                     LAFDYYSHFTSPIRRYPDVMVHRLLQFYLDGGKSVDEEKYEAKCLHSSTMEGLASNAE
                     RDSIKYMQVKYMQNHNDQEFLGVISGVTEWGIFVEIIENKCEGMVRIREIKDDYYTFD
                     EKQYALVGATTKSLLQLGDEIYVKVKNADLVKKQLDFNFIRRNE"
     gene            7093..7758
                     /locus_tag="PLQ70_06425"
     CDS             7093..7758
                     /locus_tag="PLQ70_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HQE34289.1"
                     /translation="MKKIILIVFVVISQLNYSQTTIKLDDFDEIKVFDQLNVTLVPST
                     ENKVEITGKNESSFETVNKNGVLKLRMKLTKILSGDGTKVTLYFKNIKSIDANEGSIV
                     SCAAIFKQTTIDLSSQEAAKIEVDLDVDNALIKISSGGIVSLTGKAVTQKAIINSGGL
                     FYAKDLVTSQTSVSVSAGGSADVNATTLVNAKVNAGGSISIYGKPKEIKQETFAGGTI
                     TEK"
     gene            7829..>8420
                     /locus_tag="PLQ70_06430"
     CDS             7829..>8420
                     /locus_tag="PLQ70_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQE34290.1"
                     /translation="MINDILSGIPWGIFLSFMIGPVFFILLETSIIKGFRAALVFDIG
                     VVLGDIVFIAIAYLGSYRLIKSLEDNSALFIFGGMLMLAYGIISYISLHKEKKVNTHK
                     IDNEIIKKDYLGLFIKGFFLNIINIGVLGFWLAVIISVGPKLEMQNSRMLTFFTTVIL
                     SYLLVDCIKIVLAKQLKTKMTPKNILKIKKIISIVLM"
BASE COUNT         2977 a         1172 c         1300 g         2971 t
ORIGIN      
        1 atgatgtttt tcccgttcct ggttttccgt gtaacaaaat caaacctttg tcattatttt
       61 tagataatct tttatagatg gtttggtgta tttcttgaaa gtcatcgttg taattgtctt
      121 caacttttag ttttggtttc gatattttta aagaagtggt agtaatgcct cttgctccat
      181 ttgtcaataa ggatatttct tgtggttgtt tactttttct tttcttacac ttaataattc
      241 cctcgataat tgattgaagt tttgaaagat tttgatttaa aaaaagaaat tgaacagaat
      301 tataatccat ataaaagatt ataatcattt catccaaaat gtagtaaact tgttttaatt
      361 tagatttagt tccattttca aatacttctt tttgatagaa attctccttg atttcatttt
      421 catatttatc aaagaaattg gcactcgcct tttcacaatt aatattaatt tcttcagtcg
      481 aatttggtat ggcattaaaa taaagcagaa aaagctcgta atagtttaga taaccattat
      541 tcccattatt caagaataaa ttgcttgtat ttagtttaga aattgattcc gttaagttca
      601 tccttttttt gcaaatcaaa aaaaatagat ttgattatga aacttgaaag aacagttttt
      661 taaaaataat tttttaatac ctataaacga atgaattgta tgttggtcat tgttaataac
      721 tatgtttgta agttcttgct aattttagaa gaattagcaa gaattggttg ctttaatttt
      781 taattaattc gttaaatttt gaagatctaa tataattttg ttttactttg tttacagaaa
      841 tcacttccat aaagcccata tttacaaggt tattcaaatc aacacgtgct gtataattag
      901 cgaccgaaaa tctgttttgt acctctttta cagaaaacac acattcgggt tcatcgtata
      961 aagtttttag aatttgagct tgtctctcat ttatattagg tattttaata aaactggcaa
     1021 tttgtaaatt atctttttgc tgttcttgta tgtaattttt taaagactca aatgctttgt
     1081 gcattacttc aatatgatag gtaataaaat aagataaatc attagcatca ttttcggtgt
     1141 ataaatacgc tttttcatat tggtttttag acccttgaat aattttagaa attgataaat
     1201 attctgtaag ccaatatcct ttttttaata aataccaata aaataaagct cttgctgttc
     1261 taccgtttcc atctgtaaaa gggtgtatcc aacctatcat aaaatgaata attatacctt
     1321 tgacaatggg atgaataaaa tcttcttttt ctgaattaaa aaattgacaa agttcctgta
     1381 ttagtttatt aatttgcaca aaatctggcg gcgtgtgcac cacttcactt gtagaatgat
     1441 tgacaacata aatgtcattt gaagttctaa aagcccccac ttcgttctca ttttttaatg
     1501 tgttcttaca cattaattga tgaatctcta aaaggttttc aggtgttaac tcttcatttt
     1561 tatttttcag aatatgcttg attgtagtgt aattgtttac aatcatttgt tcggacttat
     1621 taatgggttt tgtatccttt cgaagcatat cttttgcttt ttttcgagta gtgtttgcac
     1681 cttccataat actactcgaa atagcttctt ccattattga actcaccaaa tatcgagctt
     1741 tatccgattc gggaattatt ttttgtgaac ccatataccc accaatattc atatcaaatt
     1801 cgtgtagcga tttttgaata taatcggtat tgaaataatt aaaaacaacg tttgaaaact
     1861 taatttgttt gtaattaata cttctaacta atttaaccgt attccatatt tcttctgctg
     1921 ataaaccatt gactttttta tatttgactt tatcccaata taaataatta ttgtttatct
     1981 ctttaatttt agccataatt tttggcttat taagatttga aacaatggaa ttatcaaaag
     2041 taattgttgg tgtgttttca atcattttgc agatttatta aaaaacaaat gtattaaaaa
     2101 ctttttaatt cttgctaatt ttaaaagaat tagcaagaat taaaaagttt aatgatttaa
     2161 agcaaaggtt ttttgatttt tttcggacag ctaaaataat atctttgaga cttaatttta
     2221 gttaaatgga tattttatta tactattttt tgattttgaa tggaattgct tttctgttaa
     2281 tgggctacga caaacattta gcacaagctc aaaaacaaag aatttcggaa cggactttat
     2341 taagttttgc tttctttgga ggaactattg gttctggttt agcaatgctg atttttagac
     2401 ataaaatttc gaaaaaaagc tatcttttga aattttggtt tgttgtaatt gtacaatttt
     2461 taatagtttg gttatactta aaccactaaa aaaagcccag tttttagacc gagctttaat
     2521 tatttatggt gcgaagcacc tcgatttttt tttgactaat cctgaactat aaaatcctta
     2581 gaagcttctt ccctattata ttcgggttga atattaatgt gattgatttg gaatttatca
     2641 aataaaacgt gctctatttt atgcaagatt tcattaaatt cagacatttt tatgtcttct
     2701 gaacaatcga gatgcgcttc aagatgcaat tcatcatcgt tgagatgcca aacgtgaatg
     2761 tggtgtaatt tatcagtacc tggaattttg tgtacttctt cgataatttc atttatatca
     2821 atatgagttg gagtaaaaag cattaacatc tttgttgaat ctttaagtaa atcaaagcca
     2881 acaacaagta aatatattgc aattgccaat gttaaaacgc tatcgaccca aaaccactga
     2941 aaatatttca tcaataatcc accaactaaa acggctactg atgctaacat atctgtaaac
     3001 aaatgcaaat aggccgattt catattcaga tttttatcag catcattttt taataataat
     3061 accgacaaac catttgcaac aattcctata atggaaagcc aaattactaa accagattta
     3121 atgggttctg gattgaaaaa gcgttcgaca gctccataaa ccaaaaacaa agccacaatg
     3181 attaaagtca ttgcattaac aaaagcagca ataagttcag ctcttttata accaaatgtt
     3241 tgatttacag aagcttctct tttagctaat ttatgagcta cataactaaa aaccaaagaa
     3301 agaacatcag aaaaattatg caatgcatct gacaataagg ctaaacttcc agataggatt
     3361 ccaccaacaa cttgtgctac cgtaataaca atatttaaaa taatagaaaa taataaattt
     3421 ttaccttcta cttgatgatg gtggtgatgc tcgtgattat gatgctgtcc caaaatatta
     3481 acaactaatt ttatctactc tagtttgatg tcttccgcct tcaaattcgg tggtcaaaaa
     3541 agtttcaaca atttcgattg cttgttgtat tgatgtgtaa cgggccggaa tactaagaac
     3601 attagcatta ttatgtaatc gagttaaata agcaatttct tttatccaac acaatccagc
     3661 tcttactttt ggatgtttat taaccgtcat cgcaattccg tttcctgaac cacaaatcac
     3721 aattccaaaa tcggcttttc cttccgaaac atcgttggca actggatgtc caaaatctgg
     3781 ataatctaca gaagcatcgg tatcagttcc gtaattggtc acttcgtatc cttttgattc
     3841 taaatattca acaatagctt ttttatattc tggtcctgcg tggtcgtttc cgatagagat
     3901 tttcataata atttagattg aattttgtgc cgcaaattta ctgaaacaat tacttattat
     3961 tcttaatatc aaattaaata tataactatc atatttttaa tgttatagct ttttaaaaaa
     4021 atagtttcta taaaaattaa cttgaaaaat tatttcaaat gtaattttaa tgttaaataa
     4081 tggtaatgtt aatagcattt tgtaaatact tgataatcag ttaaccttaa attaacacag
     4141 aatgtttata actttgaata gaaaattaga actatttctt gtttgtattg aataaaaatg
     4201 taatccaaaa ccggaatgaa aaaaccaaat ttagtttggt tgatttttag aaaagttatt
     4261 gatagaaata aagggtttat taacaaaaat agaaatgaaa cttatttaca aaaacaatac
     4321 tttttttgtt attaactgtt gttaattgtt tttaaaaaat gcttgaaatt aaaccattta
     4381 ccttcttaaa actatgttaa taaatcgtta taaaaacaaa taactacaaa attctatatt
     4441 ttaaaataat tttattctaa ctattaacac gctataataa ccattaaaaa attaatttaa
     4501 atttcctttt ctttttgttg tttttaatat gtgttgacaa cttttaaatt tcaaacaatt
     4561 tttaaattat tgtttaattc gtctaaaatg ttttgaactc tttcaaaatc atttttgtga
     4621 actttcagat gaattccgcc ataggcaata ctagccaatg gatcaattga aattaaattc
     4681 tcattttgaa acaaaaaagg aattccttca ttttgtaaaa tagatttcaa aactatgatt
     4741 tcatgagcaa aattaaatga ggcaatggtt tgaaaatggt ccattttgta aatttttata
     4801 aagatacaaa agttatattt tagataaatc tattctctat tttctgtttt ctgttttctt
     4861 atttgtaatt ttagaccttt aagaaaagat ttatgagtaa aaaactgaga aaaccagtaa
     4921 aaaaagagaa agacttctct gaaaaaatta tcaaaatact agctcaaaat gccaataaag
     4981 catttaatta taagcaaata gctgccattt tagaaaaaga tgataccaaa agcagaaacg
     5041 aaatcatcag agatttaaag attcttgctt cctccaaaaa aataatcgaa actgaacctg
     5101 gaaaatattt agtcaaagcc attagccagg attattacga aggaacaatc gatatgactg
     5161 gtcgaaaaac cgcatatttt atttgtcctg attttgaaga agacgttttt attcccacca
     5221 ataatttaaa tagagcttta gacaaagaca aagtaaaagt ctatgtctat aatcgtagaa
     5281 aaggacgaag acctgaagga gaagtaatag aagtggttga aagagccaaa actgattttg
     5341 tgggagtaat tgatattcaa aagaattttg cttttgtttc gactgccaat ccaaaaatgt
     5401 acaccgatat ttttattcca aaagataaat taggcgaagc cgaacaaggt gatgtggttt
     5461 tagttcacat tgaagattgg cctaaaagag ccgatagtcc ttttggaacc gtagtaaaag
     5521 ttcttgggaa accaggagaa cacgatacag aaattcacgc tattcttgca gaatatggtt
     5581 taccagccga atttccggta gaagtagagg tttatgctca aaaaatagac acttcaatta
     5641 ccgaagaaga aattaataaa cgtcgggata tgcgtgatac tttaactttc acaattgatc
     5701 caaaagatgc caaagatttt gatgatgctt tgtctttcaa aaagttagac aatggaaatt
     5761 acgaaatagg aattcacatt gccgatgttt ctcattacct tcaagaagga accattttgg
     5821 atgatgaagc ttatcaacgt gcgacttcag tatatttggt agatagagta gtgccaatgt
     5881 tgcccgaagt tttatccaat tttgcttgtt cattgcgtcc gcaagaagaa aaatatacgt
     5941 tttcggctat tttcgaaatt agtgaaaaag cacaagtggt caatcaatgg tttggaagaa
     6001 cagtaatttt ttcagatcaa cgatttgctt atgaagaagc ccaatatatt atagaaatga
     6061 aagacaacat tattccagcc gaaatttcaa tcactggcac ttcctataaa gtttcggatg
     6121 aaattgtcgc agcaacgctt aaaatgcagg atttggccaa aattctgcgt cgaaacagaa
     6181 tgaataacgg cgccatttcg tttgataaag tagaagtaaa attcaattta gacaatgaag
     6241 gcgaaccaga aggtgtttat tttaaaattt caaaagatgc taatcatttg attgaagagt
     6301 ttatgctttt agcgaataga aaagtggctg aatacgttgg aaaacaaaag aaaactttca
     6361 tttatagaat tcacgatgaa ccgaatgaag ataaattaat tgcgatgcaa accgtaattg
     6421 ccaaatttgg ttataaaata gatttcagaa ataaaggtga tatttcgaaa tcattgaatg
     6481 ctttgatggt ggaagtgaat ggaaaaaaag aacagaattt aattgatact ttggcgatta
     6541 gaaccatgag caaagccaaa tattcgaccg ataatattgg acattatggt ttggcttttg
     6601 attattacag tcattttaca tcgccaattc gtcgttatcc tgatgttatg gtgcatcgat
     6661 tattgcaatt ttatttagat ggaggaaaat cagtcgatga agaaaaatat gaagcaaaat
     6721 gtttgcattc ctctacaatg gaaggtttag catcgaatgc tgaacgagat tctatcaaat
     6781 atatgcaagt gaaatacatg caaaatcaca atgatcaaga attcttgggc gttatttctg
     6841 gagtaaccga atggggaatt ttcgttgaaa ttatcgaaaa taaatgcgaa ggaatggttc
     6901 gaattcgaga aataaaagac gattattaca ctttcgacga aaaacaatat gccttggttg
     6961 gagctacaac aaaatcgctt ttgcaattag gagatgagat ttacgttaaa gtaaaaaatg
     7021 ctgatttagt caaaaaacaa ttggatttta actttattag aagaaatgaa taaaatttaa
     7081 atttatagaa caatgaaaaa gataatttta atagtttttg tggtcatttc acaattaaat
     7141 tacagccaaa ccactatcaa attagacgat tttgatgaaa taaaagtgtt tgatcaattg
     7201 aatgtaactt tagtaccttc tacagaaaat aaggtagaaa ttacaggaaa aaacgaaagt
     7261 agttttgaaa ctgttaataa aaatggagtt ctaaaattaa gaatgaaatt aacaaaaata
     7321 ttatcaggag acggaactaa agtaacttta tatttcaaaa atattaaaag tattgatgct
     7381 aacgaaggaa gtattgtgag ttgtgcagct atttttaaac aaaccactat agatttatct
     7441 tcgcaagaag cggcaaaaat tgaagttgat ttggatgtag ataacgcttt aattaagatt
     7501 tcttcgggcg gaattgttag tttaactggc aaagctgtaa ctcaaaaagc aattatcaat
     7561 tcaggaggac ttttttatgc taaagattta gtaacttctc aaacctcggt gagtgtttca
     7621 gcaggaggaa gtgctgatgt aaatgcaaca actcttgtca atgcaaaagt gaatgcaggc
     7681 ggatctattt ctatttatgg caaaccaaaa gaaataaaac aagaaacctt tgcaggtgga
     7741 acgattacag aaaaataatt tgtagtagtt agtgattagc ctttagtaac tagctaatca
     7801 ctaactacta aaaactaatt actaaaaaat gataaacgat attttatcag gaattccttg
     7861 gggaattttt ttaagtttta tgataggtcc tgtttttttt atattgctag aaactagtat
     7921 tataaaagga tttagggctg ctttagtttt tgatattggt gtggttttgg gtgatattgt
     7981 atttattgcc attgcttatt taggcagtta tcgactcatt aaaagtctcg aagacaattc
     8041 ggctttattt atttttggag gaatgttgat gttggcttat ggtattattt cgtatatcag
     8101 tttacacaaa gaaaaaaagg taaataccca taaaatagac aacgaaatta taaaaaaaga
     8161 ttacttaggt ttgtttataa aaggcttttt tcttaacatt atcaacattg gagtattggg
     8221 attttggttg gctgtaatta tttcggtggg accaaaatta gagatgcaaa actcccgaat
     8281 gctgactttt ttcacaacag taattctatc gtatttattg gtggattgca tcaaaattgt
     8341 attggccaag caattaaaaa ccaagatgac acctaaaaat atcctcaaaa tcaagaaaat
     8401 aatcagcatt gttttgatgg
//
LOCUS       DAOSNN010000099         8415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8396_length_8415_cov_5.330263, whole genome shotgun sequence.
ACCESSION   DAOSNN010000099 DAOSNN010000000
VERSION     DAOSNN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8396_length_8415_cov_5.330263"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLQ70_06435"
     CDS             complement(<1..430)
                     /locus_tag="PLQ70_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQE34291.1"
                     /translation="MELDESKYLYLLFIIPILVVIFLYNQYWKRKKQREFGDLDLIKK
                     LSPEKSVFKPILKLVVLLLALVGLIFGLVNPKMGTKMETVKREGIDIVFAMDVSKSML
                     AEDVAPSRLEKSKQIVSQIINQLGSDRIGIVAYAGSAFPVL"
     gene            complement(512..1513)
                     /locus_tag="PLQ70_06440"
     CDS             complement(512..1513)
                     /locus_tag="PLQ70_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQE34292.1"
                     /translation="MGNISFLNPEFFWLFLLIPLAGAWLFWKKNQQTANLKISSTLGF
                     KDSKSLLVKLHPILNVMRILALSSLIIAMARPRTVDISNQTKTTKGIDIVLAMDVSGS
                     MLAKDLKPNRMEALKELAADFVEERPNDRIGLVLYASEAYTKTPVTSDKAVVLEAIKS
                     IKYDNVLQDGTGIGMGLATAVNRLKDSKAKSKIIILLTDGVNNAGFIEPETAADIAKQ
                     YGIKVYTIGVGTNGMAESPYAISPSGELLFQMMKVEIDEQLMRGIARKTDGKYFRATS
                     NSKLAQIYGEINKLETTEIEELKFYDYDEKYRPFVWIALALILVEVGLRNTVYRSFI"
     gene            complement(1513..3138)
                     /locus_tag="PLQ70_06445"
                     /pseudo
     CDS             complement(1513..3138)
                     /locus_tag="PLQ70_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962312.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3187..4050)
                     /locus_tag="PLQ70_06450"
     CDS             complement(3187..4050)
                     /locus_tag="PLQ70_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQE34293.1"
                     /translation="METKDLLKKVRKIEIKTRRLSDHIFSGEYHTSFKGRGMTFSEVR
                     PYQYGDDIRAIDWNVTARYNEAHVKVFEEERELTMMLMVDISGSESFGSKNQFKKDVV
                     TEIAATMAFSATNNNDKIGLILFSDQIELYIPPKKGRSHVLRIIRELIEFEPKSHKTD
                     IAQALKFLSSTQKKKAIVFVISDFISEDYEHTLKIASKKHDITGIRVYDIREEKMPNL
                     GMVSMLDAETGVTQLINTGSKSVRINYEKYYQEKLKYFKETFSKSGAGVVNTRVDESY
                     VTKLLGYFKSR"
     gene            complement(4125..5129)
                     /locus_tag="PLQ70_06455"
     CDS             complement(4125..5129)
                     /locus_tag="PLQ70_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQE34294.1"
                     /translation="MEENTTTLDIRAINEKIERESAFIDLLTMEMNKVIVGQKHMIER
                     LLIGLLGQGHILLEGVPGLAKTLAINTLAQAVDGSFSRIQFTPDLLPADVVGTMIYNI
                     KSNDFSIKKGPIFANFVLADEINRAPAKVQSALLEAMQEKQVTIGDTTFKLDKPFLVL
                     ATQNPIEQEGTYPLPEAQVDRFMLKTVIDYPKMEDERMVVRQNLKGTYEKVKSVVSVE
                     QILRAQEAVREVYMDEKIEKYILDIIFATRYPEQYKLADLKPLISFGSSPRGSINLAN
                     AAKCYAFIKRRGYVIPEDVRAVVHDVLRHRIGITYEAEAENITSVDIINKIVNEIEVP
                     "
     gene            5441..6202
                     /locus_tag="PLQ70_06460"
     CDS             5441..6202
                     /locus_tag="PLQ70_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQE34295.1"
                     /translation="MKKLALVTGATSGIGRATARILAKNNYKVILCGRREDRLIALEK
                     ELKEYTEIHTLCFDVRDKKAVFENINSLPTEFSEIDILVNNAGNAHGLDPIQNGNLDD
                     WEAMIDINIKGLLYVSKALIPQMIERKSGHIINIGSTAGKEVYPNGNVYCATKHAVDA
                     LNKSMKMDLNPYGIRVSAIHPGMVETEFSEVRFKGDKDKAANVYKGLKPLEPEDIADI
                     IHFVVSRPYHVNISDLVVMATAQGSATIVNRETTV"
     gene            6230..7369
                     /locus_tag="PLQ70_06465"
     CDS             6230..7369
                     /locus_tag="PLQ70_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQE34296.1"
                     /translation="MINKRLLIKNLLAHNDESSFYDKKRQLNLHSREGKAKFLKHICA
                     LSNSNPANNSYIVVGVEDHDNEIVGDDFFDDSRIQNLVNAYLENPPKIQYENVPFPNL
                     PKDKVVGLVTIKANSKISHFKKGIHTIPVNSVFIRRGSNTIPIEEEIEKNYQNTETVI
                     GIENNSRNSIGYTLDGVIDFMNFRHKDMAPKYKVFKELFVICWAGVPKKSRDKTFLSR
                     VDIELINEQIKLFYSAQDVVTITFDEDSFTIIEYVPLGLNDKTSFYPLEKQTLLFHDN
                     GYYKIDREFLFQPPEFNKKMLFHIYNSNISLLNKLQKGLSLTEREQKDLENLPSTFMI
                     CYLNGFEDAKQKLIDAKPLLKPFTSVYLSFKEALRILRKMKYDVQ"
     gene            7430..7870
                     /locus_tag="PLQ70_06470"
     CDS             7430..7870
                     /locus_tag="PLQ70_06470"
                     /inference="COORDINATES: protein motif:HMM:NF025471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HQE34297.1"
                     /translation="MMKKLTYRGTESNNFLYFSFDAENFDTEKITEELNIQPTSLMII
                     KELIPKSTAWKYQIDAGNEIDLETFLEKLINIFEPKIEIINRLKDEFNLSTRIQFVID
                     IDINPDSSTPYFGLNKRAINFLSRTETEVDFDLYKTDTIGLLNN"
     gene            7980..>8415
                     /locus_tag="PLQ70_06475"
     CDS             7980..>8415
                     /locus_tag="PLQ70_06475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQE34298.1"
                     /translation="MRTLVIGDIHGGLRALHQIMERANVTPKDTLIFLGDYVDGWSQS
                     PQVIDYLIELKTTHNCIFILGNHDQLLLKWLDESIDNPMWYKHGGEATVLAYAKTSNE
                     TIQKHIKFLKSLDNYYLDEQNRLFVHAGFTNMNGVAYEFFPEL"
BASE COUNT         2641 a         1560 c         1374 g         2840 t
ORIGIN      
        1 gcaaaaccgg aaaggcactt ccagcatagg caacaattcc tattctgtca ctacctaatt
       61 gattgatgat ttgcgaaaca atttgtttgc ttttttctaa tctgcttggg gcaacatctt
      121 cggcgagcat acttttggaa acatccatgg caaaaacgat atcgattcct tctcgtttta
      181 cggtttccat tttggtgccc attttaggat ttaccaatcc aaaaattagc cctactaatg
      241 ccaaaagcag tacgaccaat tttaaaatgg gtttaaaaac cgatttttct gggcttaatt
      301 tctttatcaa atctaaatcg ccaaattcgc gctgtttttt tcttttccaa tattgattgt
      361 atagaaaaat caccaccaaa atgggtatta taaaaagaag atataaatat tttgattcgt
      421 ctaattccat tattcagatt tcaaattgta gatttcagat ttcagattcc actttgaaat
      481 ttaaaaccta tccttactgt ttttactatt tttaaataaa actcctgtaa accgtatttc
      541 gtaatcccac ctcaaccaaa atcaaagcca atgcaatcca aacaaaaggc ctgtattttt
      601 cgtcgtaatc ataaaatttt agttcttcga tttctgtggt ttccagttta tttatttcgc
      661 cataaatctg ggccaatttg ctgttgctcg tggctctgaa atattttccg tctgtttttc
      721 gggcaatacc tctcattaat tgttcgtcaa tttctacttt catcatttga aataaaagtt
      781 ctccacttgg cgaaattgca taaggagatt cagccattcc gtttgtcccg acaccaatag
      841 tatatacttt tatgccgtac tgtttggcaa tatctgcagc ggtttctggt tcaataaaac
      901 ccgcattatt gactccatcg gtcaacaaaa taataatttt acttttggct ttactatctt
      961 ttaatcggtt tacggctgtt gccaatccca ttccgattcc tgttccgtct tgcagaacat
     1021 tgtcgtattt tatacttttt atggcttcta aaactactgc tttgtcactt gtaactggtg
     1081 ttttggtgta ggcttcagaa gcatacaata ccaatccaat tctatcattt ggcctttctt
     1141 caacaaaatc agcagctagt tctttgaggg cttccattcg attgggtttc aaatctttgg
     1201 cgagcatact tccagaaacg tccattgcca agacaatatc tatccctttt gtagttttgg
     1261 tttgattact aatatcaact gttctgggtc ttgccattgc aataatcaag gaacttagcg
     1321 ccaaaattcg catcacattc aatattggat gcagttttac caatagcgat ttagaatcct
     1381 taaaccctag agttgaactt atttttaagt tagccgtttg ttggtttttt ttccaaaaca
     1441 gccacgctcc tgctaatgga attagaagaa ataaccaaaa aaactctgga tttaaaaaac
     1501 ttatatttcc cattattgtt ttgtgttttc aagttccaca gagttcataa ttcgatctga
     1561 aatttgattg gcatattcat cgccttcttc gtgcaaaatg ataatttgct gcaaaccacc
     1621 attttgacta aacaataaaa tttcataata tactttggta cttgttttgg taatctcgtt
     1681 tattttagaa aaagtaccat atcctttgat gcccttaata ccttctttgg tttcgtattc
     1741 ttcttgtttt acaatcatat tttgggctcc ttgcgattcc accatttgaa tagaaccttc
     1801 taatgatttc gataaatcca gttgagtttc ctgtttgaaa ctaaaggtag aaaccattat
     1861 ataaaaatta tctataaaac ttccgtaagc aaaagattgc atttccttaa tcaatgccat
     1921 tccttctttg ggtaatgatt tcgacaaatc aaccctttta agcactttag gagtttcaat
     1981 tctcactgtt ggatttccat attcactttt aacccattcg ccttctaaca attctttgga
     2041 cggatgccct ataatattgt ctttgacata atcaaaacct ttggtggcaa taaaataagt
     2101 cagcgtaaca aaaagcaaca taaataccga gacaactgta ataaggatgc gtttggttct
     2161 ttgctttttc aactctcgtt tcaattgttc ttgacgctgc atttcgttaa gcaacatttc
     2221 ctcatcactt tcaaccacta caggaatcgc tttatccaaa gtaacaatcg ctctttctaa
     2281 cttttttcta tcttcggtaa tttcaaaatc taatggtttt gatttggcaa attttactaa
     2341 atcggcttgt tttaaaacgc caagcaaatt atcaaaagtg tctttggaaa gattcatttt
     2401 ctttttctgg gacaccattt tcagcccttc aatcagttca gaagtggtgc tctccattgc
     2461 tggaatttcg atagcttctt cgatataatt tcgaacaata tcggtcaatt cactgtaata
     2521 agctttgact tctccgtgtt gccaaagttc ttttttctcc aaattattga gcaaacttgt
     2581 cgctttttcg attggggttt tgtaaatttc tttttcaatg gtcttttttt gacgtttttt
     2641 ggtgtaccaa taaatgaagg ctccaaaacc tgcaattaag actagtccca aaagatattt
     2701 ccaccaatcg ctagattcat ttgctggaac aatatccttg atgtcataca ttttttgttt
     2761 taatgtatca actacaagat tggcaacctc aacttatatt gaatcggaca aaaatgcttt
     2821 tttgttaatc aaaatcttga tactcggtat agtatatcta cctgaatcga attgagttaa
     2881 accgtatttt ttgattaatt cgtatcggtc atttttcttg atagtgtcaa tcggatagga
     2941 ctgaatcact tcgagtgggc caaagtttct cagattcgga aaaaccactt tcgacaaagt
     3001 atcaacattg gtttttaaag ttagcttgaa ctcggcaccg attttatttt tggtggtatc
     3061 aatgctggtt tgcacctgtt tttgtgcaaa aacagtggcg gaaagcagca gtaataataa
     3121 ataaagttta attttcattt gtttctattt aaccctaaca gggttttgaa ccctgttagg
     3181 gttagtttaa cgggatttaa aataacctaa taatttcgtt acataacttt cgtcaactct
     3241 tgtgtttaca actcctgcgc ccgatttact gaacgtttcc ttaaaatatt tcagtttctc
     3301 ctgatagtat ttttcataat tgattcgcac cgattttgaa cccgtattaa tcaattgagt
     3361 cactcctgtc tcggcatcca gcatagaaac cattcctaga tttggcattt tttcttctcg
     3421 aatatcatac actcgaatac ctgtaatgtc gtgttttttg gaagctattt tcaaggtatg
     3481 ctcgtaatct tctgaaataa aatcggaaat aacgaacaca attgctttct ttttttgggt
     3541 actcgacaag aatttcaagg cttgagcaat atcggttttg tgacttttgg gttcaaattc
     3601 gattaattct cgaatgattc tcaaaacgtg tgaacgccct tttttgggtg gaatatacaa
     3661 ttcgatctgg tcagaaaaca aaatcaaccc tattttatca ttgttatttg tagccgaaaa
     3721 agccattgtt gcggctatct cggtaacaac atcttttttg aattgatttt tagacccaaa
     3781 actttctgaa cccgaaatat ctaccatcaa catcatcgtc aattcgcgtt cttcctcaaa
     3841 tactttgacg tgagcttcgt tgtaacgagc tgttacgttc cagtcaatag cacgaatatc
     3901 atcgccatat tggtacggac gcacttcgct aaaagtcatt ccacgtcctt tgaacgaggt
     3961 gtggtattcg cccgaaaaga tatgatcgct caatcttcgg gttttaattt ctatctttcg
     4021 tacttttttt aagaggtctt ttgtttccat tgtatgtggt tgttggttgt cggttgtcag
     4081 ttgttggaaa aaaccatcaa ctatcaacca aaaactatca acttttaagg tacttcaatc
     4141 tcgtttacga ttttgttgat gatgtcaacg gaagtaatgt tttcggcttc ggcttcataa
     4201 gtgattccaa ttctgtgacg caacacatcg tgaactacgg cacgaacatc ttctggaatt
     4261 acataaccac ggcgtttgat aaaagcataa cattttgcgg cattagccaa gttgatgctt
     4321 ccacgaggcg aagaaccaaa actgattaat ggtttcaaat cggccaattt gtattgctct
     4381 ggataacggg tggcgaagat aatatcaaga atatatttct ctattttttc gtccatataa
     4441 acctcacgaa cggcttcttg cgcccgcaaa atttgttcta cagagacaac tgattttact
     4501 ttttcgtaag ttccttttag attttgacgc actaccattc tttcgtcttc catttttgga
     4561 taatcaatca cagttttcaa cataaaacga tcgacttgtg cttctggcaa aggataagtt
     4621 ccttcctgct cgattggatt ttgagttgct aaaactaaaa atggtttgtc taatttgaaa
     4681 gtggtgtcgc caatagtcac ttgtttttcc tgcatcgctt ctaacaatgc cgattgcact
     4741 ttggctggcg cacggttaat ctcatctgca agaacgaaat tagcaaaaat aggtcctttt
     4801 tttatagaaa aatcattgga tttaatattg taaatcatgg ttcccactac atcggcaggc
     4861 aataaatctg gagtaaactg aattcgactg aacgaaccat caacggcttg ggccaaagta
     4921 tttatggcta atgtttttgc caaacctgga acaccttcaa gtaaaatatg tccttgtccc
     4981 aaaagcccaa ttaacaatcg ctcgatcata tgtttttgac ccacgattac tttattcatc
     5041 tccatggtta gcaaatcgat gaaagcactt tctctttcta ttttttcatt gattgctcta
     5101 atgtccaaag tcgttgtatt ttcttccata ttcgtatttt aaatgctgta aattgtgttc
     5161 gttctttttg acggtgcaaa ttgaattttt ttttagaagt acgatgttaa aaaatggtta
     5221 aaacttccga caagtgccca attttaagga tgttttgtga tagtattttc atatttttaa
     5281 agtttcaaaa aaaaatgttc caaaatatag aattcactac tatttttata gcattttgac
     5341 cttgaccgaa accctttttt taaaataaaa ttatcactat gttttaggaa aaccctagaa
     5401 ataaaacatt tatttagcat ttataaattt aaaatagctt atgaaaaaat tagccttagt
     5461 aactggagca acgagcggaa ttggaagagc aactgcaaga atattggcca aaaataatta
     5521 taaagttatc ctttgcggaa gaagagaaga tcgattgata gcccttgaaa aagaacttaa
     5581 ggaatataca gaaatccata cattgtgctt tgatgttcga gataaaaagg ccgtttttga
     5641 aaacataaat tcgttaccta ctgaattttc tgaaatcgat attttggtta acaatgcagg
     5701 aaacgcacac ggtttagacc ccattcaaaa cggtaattta gacgattggg aagcgatgat
     5761 agacatcaac ataaaaggac ttttgtatgt ttcgaaagcc ctaattccac aaatgattga
     5821 acgaaaatcg ggtcatatta tcaacattgg ttctacggct ggaaaagaag tatatccaaa
     5881 cggaaatgtg tattgcgcca caaaacacgc tgtagatgct ttgaacaaaa gtatgaaaat
     5941 ggacttaaat ccctacggaa tcagagtgag tgctattcat cctggaatgg tagaaaccga
     6001 gtttagcgaa gtccgattta aaggagataa agacaaagcg gccaatgttt acaaaggtct
     6061 taaaccctta gaacccgaag atattgccga cattattcat tttgtagttt ctagacctta
     6121 tcatgtaaac atttcagatt tagtagtaat ggctactgca caaggctcgg ctacaattgt
     6181 aaatcgagag acaaccgttt aattctaaaa atctaccaac ccttttgcaa tgattaacaa
     6241 acgtctttta attaaaaacc tattggctca caacgatgag agcagttttt atgacaaaaa
     6301 acgccagttg aatttgcatt caagagaagg aaaagcgaag tttttgaaac atatttgtgc
     6361 cttatccaac tcgaatcctg ccaataattc ctatattgtt gtgggagttg aagaccacga
     6421 taatgaaatt gttggtgatg acttttttga cgacagtcgc attcagaatt tagtaaatgc
     6481 ttatttagaa aatccaccca aaattcaata cgaaaacgtg ccttttccta atttacccaa
     6541 agacaaagta gtaggattag taaccataaa ggccaatagc aaaatctctc atttcaaaaa
     6601 agggattcat acgattcctg tcaacagtgt ttttattcga cgtggcagca acacgattcc
     6661 aattgaagaa gaaatcgaaa aaaattatca aaacacggaa accgttattg gtattgaaaa
     6721 taattccaga aatagtattg gttatacgct ggatggcgtg atcgatttta tgaattttcg
     6781 ccacaaagat atggcaccca aatacaaagt tttcaaagaa ttatttgtca tttgctgggc
     6841 cggagttcct aaaaaatccc gtgacaaaac ctttctttcc cgagtggata ttgaactcat
     6901 caacgagcaa atcaagctgt tttactcggc tcaggatgtg gttacaatta cttttgacga
     6961 agattctttt acgattatcg aatatgttcc tttgggcttg aatgacaaaa cgagctttta
     7021 tcctttagag aaacaaaccc tgctttttca cgacaatggc tattataaaa ttgatcggga
     7081 atttctgttt caaccgccag aattcaataa gaaaatgttg tttcatattt acaattccaa
     7141 tatttcattg ttgaataaac tccaaaaagg gctatcatta acggagcgag aacagaaaga
     7201 tttagaaaat ttaccctcga catttatgat ttgttacctc aacggatttg aagatgccaa
     7261 gcaaaaattg attgatgcca aaccgctttt aaaacctttc acttctgtat atttgtcgtt
     7321 taaggaagcg ttacggattt tgagaaaaat gaagtatgat gtgcagtaga aatttaaccg
     7381 caaattcgca aattaaatag ttgtgtgcga ttttaaacaa ccaacagaaa tgatgaaaaa
     7441 actaacatac agaggaactg aatccaacaa ttttttgtat ttctcattcg atgcagaaaa
     7501 tttcgacaca gaaaaaataa cggaagaatt gaatattcaa ccgacttctt taatgataat
     7561 aaaagaactt attcctaaat caactgcttg gaaataccaa attgatgcag gaaatgaaat
     7621 tgatttagaa acctttcttg aaaaactaat taatattttt gaacctaaaa ttgaaatcat
     7681 aaatagacta aaagacgaat ttaatttgtc cacaagaatt caatttgtaa ttgacataga
     7741 tatcaaccct gattcttcta ccccgtattt tgggctgaac aaaagagcaa taaatttttt
     7801 atcaagaaca gaaacggaag ttgactttga tctttataaa actgatacaa ttggattatt
     7861 aaataactaa aaagtaggtt atgatgcatt agttatattc ctaaaaactt atgatggcaa
     7921 cagccattta aattaataat tttcgataaa ttatttaaat aaaaaataaa caaaacagaa
     7981 tgagaacact agtcataggc gacattcacg gtggtttgcg ggcgctgcac caaattatgg
     8041 aaagagccaa tgttacccca aaagacacct tgatttttct gggagattat gttgatggtt
     8101 ggagccaatc gccacaagtg attgactatt taatcgaatt gaaaaccaca cacaattgca
     8161 ttttcatcct tggcaatcac gaccaattat tactaaaatg gcttgatgaa agtattgata
     8221 acccaatgtg gtacaaacat ggtggagaag caaccgtttt agcctatgct aaaaccagca
     8281 atgaaaccat tcaaaagcac attaaattcc taaaatcatt agacaattat tatctagacg
     8341 aacaaaatcg tttatttgtt cacgctggtt ttaccaatat gaatggtgtg gcttacgaat
     8401 tttttcctga acttt
//
LOCUS       DAOSNN010000100         8329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8508_length_8329_cov_6.547136, whole genome shotgun sequence.
ACCESSION   DAOSNN010000100 DAOSNN010000000
VERSION     DAOSNN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8508_length_8329_cov_6.547136"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="PLQ70_06480"
     CDS             <1..97
                     /locus_tag="PLQ70_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatE protein"
                     /protein_id="HQE34299.1"
                     /translation="FVLEALDNWKKIQLTDGTQGWIDKNAIREVK"
     gene            complement(133..657)
                     /locus_tag="PLQ70_06485"
     CDS             complement(133..657)
                     /locus_tag="PLQ70_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQE34300.1"
                     /translation="MSFLDIILGALLVIALFKGIKNGLFVELASLVSLVLGIYLAIKF
                     SFLARIMLSAITHWNPKTIQITAFIITFLLVVIGISLLAKFLTSVADFAFLGVMNKLG
                     GGFFRLLKTILIISIFLNLFEKINFNNTFAKKETLDKSLFFRPIQKTAGFIYPSIEKW
                     YEDLKKSKHTSDGR"
     gene            781..1137
                     /locus_tag="PLQ70_06490"
     CDS             781..1137
                     /locus_tag="PLQ70_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34301.1"
                     /translation="MSRDIQLKERWEQLVTILSDQFSQGEDLDLDAIIYLIGVQELGK
                     VHKTFEKDEKLNLMHIAICRLLEPYGFYEFEFFDEDGWPHYKVKEELPPLKAGEQSVL
                     MKEAIVNYFIEKELID"
     gene            1249..2268
                     /gene="pheS"
                     /locus_tag="PLQ70_06495"
     CDS             1249..2268
                     /gene="pheS"
                     /locus_tag="PLQ70_06495"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQE34302.1"
                     /translation="MIDKIKEYIGEAQAFSTQNKEELEAFRIKFLGSKGLLKDLFAEF
                     KNVPNDQKKEFGQVINLLKSSAEEKVKSIQESLESKEESKGFYGDLTRSAEPVIIGSR
                     HPISLVKNQIIDIFSNVGFNVSEGPEIEDDWHNFTALNLPEYHPARDMQDTFFIQTNP
                     DILLRTHTSSVQVRYMENNKPPIRTISPGRVFRNEAVSSRSHCIFHQVEGLYIDKDVS
                     FADLKQTLLYFTKEMFGKSKIRLRPSYFPFTEPSAEIDIYWGLKTETDYRITKGTGWL
                     EIGGCGMVDPNVLKNCNINPDEYTGFAFGMGIERIAMLLYQIGDIRMFYENDVRFLEQ
                     FKASI"
     gene            2350..2757
                     /locus_tag="PLQ70_06500"
     CDS             2350..2757
                     /locus_tag="PLQ70_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34303.1"
                     /translation="MKKDIIIPEVENVFLAAVQEWSDDFMEKVWYIYLVNDSDFQLDS
                     VMVVSKAFGTIDGEMKKTSLLRHAFIEVPAVTFVKVEMVEKSVLVLNNEFMVTFFIGN
                     TLYDRKYIFKANSITPDYVEEVPLLFVDGVIVR"
     gene            complement(2786..3709)
                     /locus_tag="PLQ70_06505"
     CDS             complement(2786..3709)
                     /locus_tag="PLQ70_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQE34304.1"
                     /translation="MKTRLKNAFASRINAIGLPILALCWVCFFWGTTWIASKEGVRHM
                     PALQLAGIRQFIGGILYVTFFLYKKAPWPKGKQWKAIFILSILNFVLSNGLSTWGVKY
                     ISSGLGAIIGAIFPLWIVFITLFRGERIARLSVLGLIVSFSGVCVVFFDHLADLLNPD
                     FRFGILLSLFATVSWAFGSLYTKKKAASFNPYFSLGLQMLISSCITLAIVGATGTGVT
                     IASIPANSWWAIGYLVIIGSVLTFIAFIYMLQRLPPEINSLYAYVNPIVAVLLGTIIF
                     VEQLTMAIAIGGGITLGGLYLVNYSMRKSKM"
     gene            complement(3711..4514)
                     /locus_tag="PLQ70_06510"
     CDS             complement(3711..4514)
                     /locus_tag="PLQ70_06510"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE34305.1"
                     /translation="MTQISILGCGWLGLPLAKVLLGNRFSVKGSTTSVDKISILENLG
                     IQAFQMELAETKIQGEVDSFLENSKILLIDIPPKLRGISKENFVAKIENAIPFIEKSS
                     VENVIFISSTSVYGEDNLVVTEETKLNPDTESGKQLVQAEQILQSNKNFKTTILRFGG
                     LIGEDRHPIKFLAGRKNIENPYAPINLIHQDDCIGIILKIIESNSWNETFNAVTPFHP
                     TRKEYYTQKAIDLKLDLPEFNVENSILGKTISSLKIENLLKYSFIKPNL"
     gene            complement(4556..5302)
                     /locus_tag="PLQ70_06515"
     CDS             complement(4556..5302)
                     /locus_tag="PLQ70_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34306.1"
                     /translation="MKHLPKINLFYWALIISANTMGETAGDLISQTFNLGYAGGTIAL
                     IALFVLVLIVSISSKSQKPLLYWTAITLASTAGTTISDFISRSFFHLQLGFSENQGYS
                     FGTMILVIALLGTFGIWKYHSKTNTIENGLNQRTEFLYWIAILTSSTLGTSFGDLLAH
                     NTPLGFDGGTFLLLGLLSIIVGLFYFSKISRELLYWFAIILTHPIGATMGDYLTKPEG
                     FNLGNINASLLLVVVFIIVIATGQLTKKRK"
     gene            complement(5407..7797)
                     /locus_tag="PLQ70_06520"
     CDS             complement(5407..7797)
                     /locus_tag="PLQ70_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQE34307.1"
                     /translation="MKKNYSSILSVLFILGFLGFVFFTMMPQWTSAEDDSLSEFSTQR
                     ALKQVKNISQKPHFVGSENHDVIANYLVKELQNMGLETSIQEGNSFSDWGNLTHCKNI
                     LARIKGSNSNKALLLLSHYDSAPHSYSFGASDAGSGVATILESIRAFVYNKKQHKNDI
                     IILFTDAEELGLNGAALFVTQHHWAKEVGLVLNFEARGSSGPSYMLMETNEGNAGLVN
                     QFSAANVQFPISNSLMYSVYKMLPNDTDLTVFREQGNIQGYNFAFIDGHYNYHTAQDD
                     LNHLSKNTLAHQGKYLMPLLDYFSNADLNSTQATEDNVYFSIPFSFISYPFSWVWPMV
                     IIALVILLFLIFIGIGKRILFFPEMTRGFIPFLGSLIVSGLVTFLGWKLLLILYPQYN
                     DLLNGFTYNGHDYIAAFTLLSLAFCFAFYQIFSFKKVTMNHFIAPLFFWILINGLLAF
                     FLKGAGFLIIPVFSGLLMLASFVLTQKSKWVLNLILGIPALLILAPFIQMFPIGLGLK
                     VLFGSAILTVIAFGLLLPIFGSFIRKGKASLLFLLLAIGFFAKAHYFSGYEAGKAKSN
                     SLVYILDADKNKATWATYDINLDEWTKTYLGQNPRDAAALSSIILPSKYKTEFTFTAD
                     APMKNLSRPVVEFLKDSVVGANRYLKIRITPTRKVNRYDILANENFVIQNFKANGLSL
                     LGQKGSKYERKGKKLLSYYVVDQLPLEIEFSVATSSVLDLDLLESSFDLMNNPLFKMA
                     KRADWMMPTPFVLNDAVIIKQKIKPSPKTVKPVILKVWKRFKKDSLVVEKDSLSKP"
     gene            complement(7816..8166)
                     /locus_tag="PLQ70_06525"
     CDS             complement(7816..8166)
                     /locus_tag="PLQ70_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34308.1"
                     /translation="MDKSCLECGDKIVGREDKKFCSDGCRNAYNNKINKDSTNFMRNV
                     NNKLRKNYRILSALNVDGKGKTTKSKLLSKGFDYEFFTNILQTKTGNTYYFVYDQGYR
                     ALEDDYYMLVKKEI"
BASE COUNT         2747 a         1538 c         1520 g         2524 t
ORIGIN      
        1 ttttgttttg gaagctttag acaattggaa aaaaattcaa ttgaccgatg gaacccaagg
       61 ctggatagat aaaaatgcaa ttagagaagt gaagtaaaag tgttcagttt tcagtagtta
      121 gtattcagtg tgctatctgc catctgaagt atgtttactt tttttaaggt cttcatacca
      181 tttttcaatc gaaggataaa tgaatcccgc tgttttttga ataggacgaa aaaacaacga
      241 tttgtctaaa gtttcttttt tggcaaaagt gttgttgaag tttatttttt cgaaaaggtt
      301 caaaaagata ctgataatta agattgtttt cagtagtcta aaaaagccac ctcccagttt
      361 gttcattaca cccaaaaacg caaaatcagc aacgctggtc aagaattttg ctaagagtga
      421 aataccaata actactagta gaaatgtaat gataaaagct gtaatctgga ttgtttttgg
      481 attccaatgt gtaattgccg acaacatgat ccttgctaaa aacgaaaact ttatagcaag
      541 ataaattcct aaaaccaagg aaactaatga agctaattct acaaaaagcc cgttttttat
      601 gcctttgaat aaggcaatga caagcaaagc acccaaaata atatctaaaa aactcataat
      661 ttaaaattaa agagaaaaaa taaagacaaa gataaaagaa ttatgttggt atctttgccc
      721 aaatgacttt tatgtttttt gaactcataa ttcataattc ataattcata attcaacaaa
      781 atgtctagag atatacaact aaaagaacgc tgggaacagc ttgttacgat actttccgat
      841 caattttcgc aaggcgaaga cctcgatttg gatgctataa tttatttaat tggcgtacaa
      901 gaactaggaa aagtgcataa aactttcgaa aaagacgaaa aactcaacct gatgcacatc
      961 gctatttgcc ggttattgga accttatggt ttttatgaat tcgaattttt tgacgaagac
     1021 ggttggccac attacaaggt aaaagaggaa ttaccgccat taaaagcagg agaacaatcg
     1081 gttttgatga aagaagcgat tgtgaattat tttattgaaa aggaacttat tgattgatga
     1141 ttgaagatta acgattgcag attttagatt tatcgaggaa actttaaact ttaaacttta
     1201 aacctcaaac aaatttctta attttgcact ctcaattatt ggatgaaaat gatagacaag
     1261 ataaaagaat atattggcga agcacaggct ttttcaacac aaaacaagga agaactagaa
     1321 gcatttagaa tcaaatttct gggaagcaaa ggacttttaa aggacttatt cgccgaattc
     1381 aaaaacgtac caaacgatca aaagaaagaa tttggacaag taattaattt gctaaaatct
     1441 tcggccgaag aaaaagtaaa atccattcaa gaatctttgg aaagcaaaga ggaaagcaaa
     1501 ggattttatg gcgatttaac ccgctctgcc gagcctgtaa ttattggttc gcgtcaccct
     1561 atttcattgg tcaaaaacca aattattgac atcttttcta atgttggttt caacgtttcc
     1621 gaagggccag aaatcgaaga cgattggcat aattttaccg cattgaactt gccagaatac
     1681 catccggcac gtgatatgca ggacactttt ttcatccaaa ccaatcccga tattttgttg
     1741 cgcacccaca cttcatccgt gcaagttcgt tatatggaaa acaacaaacc gcctattcga
     1801 accatttcgc caggaagagt ttttcgtaac gaagcggttt cgtctcgttc gcactgtatt
     1861 ttccaccaag tggaaggttt gtacattgac aaagacgttt cctttgccga tttaaagcaa
     1921 acgcttttat atttcaccaa agaaatgttc ggaaaatcca aaattcgctt gcgtccatct
     1981 tattttccat ttacagaacc aagtgcagaa attgatattt attggggatt aaaaaccgaa
     2041 accgattata gaattaccaa aggaactggt tggttagaaa ttggtggttg tggaatggtt
     2101 gatcctaatg tcttgaaaaa ttgcaacatc aatcctgatg aatatactgg ttttgccttt
     2161 ggaatgggaa tcgaaagaat tgcaatgttg ctctatcaaa ttggcgatat tcgaatgttc
     2221 tacgaaaacg atgttcgatt cttagagcaa ttcaaagcga gtatataaaa aaagtcacga
     2281 gtgaatagtg attagtaatt agttctgctt cttactaatc actattcact ttttactaat
     2341 cactatttta tgaaaaaaga catcatcatt cccgaagtag aaaacgtatt ccttgccgca
     2401 gtccaagaat ggagcgacga ttttatggaa aaggtttggt atatttattt agtaaacgac
     2461 agcgattttc aattggatag cgtaatggta gtttccaaag ctttcggtac tattgatggc
     2521 gaaatgaaaa aaacttcact tttgcgccac gcttttatcg aagtgcctgc ggttactttt
     2581 gtaaaagtag aaatggtcga aaaaagtgtt ttggtactca acaacgagtt tatggttact
     2641 ttcttcattg gcaacacgct ttacgaccga aaatatattt tcaaagccaa ttccatcact
     2701 ccagattatg tggaggaagt gcctctatta tttgtagatg gtgtgattgt gaggtagatt
     2761 agacttaatt aaaaaaaact actttttaca tttttgactt tcgcatcgaa taattgacta
     2821 aatacaatcc gccaagtgta attccgccac caattgcgat tgccattgtg agttgttcca
     2881 caaaaattat agtacccaac aaaacagcca caattggatt cacataagca taaaggctat
     2941 ttatttctgg cggtaacctt tgcagcatat aaataaaagc gataaatgtc aagacggaac
     3001 caatgattac taagtatcca atggcccacc aagaatttgc aggaatcgaa gcgatagtta
     3061 ctccagttcc agtcgcacca acaattgcta aagtgatgca actcgaaatt agcatttgca
     3121 atcccaaact gaaataaggg ttgaagctgg ccgctttttt cttggtgtat aaactcccaa
     3181 aagcccaact tacagttgca aatagcgaaa gtaaaatacc aaatctaaaa tcggggttca
     3241 ataaatcggc aagatgatcg aaaaaaacaa cacaaactcc tgagaaactt actatcagtc
     3301 ccaagacaga taagcgagcg attcgttctc ctctaaaaag agtgataaaa acaatccaaa
     3361 gagggaaaat tgcaccgatg atggcgccca aaccactact aatgtatttt accccccaag
     3421 tgcttagccc attactcaag acaaaattga gaatgcttag aataaatatg gctttccatt
     3481 gttttccttt tggccaaggc gcttttttgt ataaaaagaa agtgacataa agaattccac
     3541 caataaattg tctaatccct gcaagttgca atgctggcat atgtctcaca ccttcttttg
     3601 aagcaatcca ggttgtgccc caaaaaaaac acacccaaca caaagctaaa atgggtaatc
     3661 caatggcatt aattctagat gcaaaagcat tttttagtct agttttcact ttataaattt
     3721 ggttttataa acgaatattt taatagattt tctattttca aactcgaaat ggtctttccc
     3781 aaaattgaat tttcaacatt aaattcgggt aaatctagct tcaaatcaat cgctttttga
     3841 gtataatatt cctttcgggt cggatgaaaa ggcgttacgg cattgaaggt ttcgttccaa
     3901 gaatttgact caataatctt tagaattatg ccaatgcaat cgtcttgatg aatcagattg
     3961 atgggagcat atgggttttc gatatttttt cttcccgcca agaattttat gggatgtcgg
     4021 tcttcaccaa tcaagccgcc aaaacgaaga atagtagttt tgaaattctt attgctttgc
     4081 aaaatttgct ctgcttgaac taattgtttg ccactttcgg tgtcaggatt tagtttcgtt
     4141 tcttccgtca caactaaatt atcttcacca taaaccgagg tcgagctaat gaaaattacg
     4201 ttttcaacag acgatttttc tataaacgga atcgcgtttt caattttggc aacaaaattt
     4261 tcctttgaaa tacctctcaa tttgggagga atgtcaatta gtaagatttt agagttttct
     4321 aaaaaagaat cgacttctcc ttggattttt gtttcagcta attccatttg gaaagcctgt
     4381 attcccaagt tttccaagat cgaaattttg tcaacagaag tcgtcgaacc ctttacggaa
     4441 aatctgtttc cgagcaaaac tttcgccaaa ggcaaaccca accaaccgca acccagaata
     4501 ctaatttgtg tcattttgaa aaaagcgtta tcagatattg gttaaatgaa tagaattatt
     4561 ttcgtttttt tgttagttgt cctgtagcaa taacgattat aaacacaaca actaaaagca
     4621 aactggcatt tatgtttcct aaattgaaac cttcgggttt tgtcaaatag tcacccattg
     4681 ttgcaccaat tggatgtgtc aaaatgatgg caaaccaata taaaagttct ctggatattt
     4741 tgctgaaata aaaaagccca actattatgg agagtaatcc taatagaagg aatgttcctc
     4801 cgtcaaaacc taatggtgta ttgtgcgcta acaaatctcc aaacgaagtt cctagtgtac
     4861 ttgaagttaa aatggctatc caatataaga attcagttcg ttggttgaga ccattttcta
     4921 tagtgtttgt ctttgaatga tatttccaaa taccgaaggt tccaagcaaa gcaataacta
     4981 aaatcattgt gccaaagctg taaccttgat tttcagaaaa gcctaattgt aaatgaaaga
     5041 atgaacggct aataaaatcg gaaatggttg ttcctgctgt gcttgccaga gtaatggctg
     5101 tccaatacaa aaggggtttt tggctttttg aggaaataga aactatcaaa accaatacaa
     5161 aaagtgctat taaagcaatt gttccgccag cataacctaa attaaatgtt tgggatatta
     5221 aatctccagc ggtttctccc atcgtgttgg cactaataat taatgcccaa taaaagagat
     5281 ttattttcgg taagtgtttc atttgtaagt tgaacttttt tagaaattca aaagtatgta
     5341 aaataaacct caattaagag ttattggatt tggtttatac gtcatcacaa attgtgacca
     5401 catacattac ggtttgctca aactatcttt ttccaccacc aaactatctt ttttaaatct
     5461 tttccaaact tttaaaatta ctggtttaac cgtttttggc gatggtttta ttttctgttt
     5521 aataatcacg gcatcattca atacaaaagg cgtcggcatc atccaatcgg ctctttttgc
     5581 cattttaaat aatggattgt tcatcaaatc gaaagaactt tccaataaat ctaaatccaa
     5641 aacactcgaa gtggcaacac tgaattctat ttccaacgga agttgatcga ccacataata
     5701 actcaacagt tttttgcctt ttcgttcgta tttggaacct ttttgtccca ataacgacaa
     5761 gccattggct ttgaaatttt gaattacaaa attttcattg gccaaaatat cataacgatt
     5821 gacttttcgg gttggcgtga ttcttatttt caaatagcga ttggcgccaa caacgctgtc
     5881 ttttaggaat tccacaacgg gtctcgaaag gtttttcatc ggcgcatcgg cagtgaaagt
     5941 aaattcggtt ttgtatttgc ttggtaagat aatagagctt aatgctgcgg catctctagg
     6001 gttttggcct aaataggttt tggtccattc gtctagattg atgtcgtaag tcgcccaagt
     6061 agctttgttt ttgtcggcat ccaaaatata aaccaaactg tttgatttgg ctttccctgc
     6121 ttcataaccc gagaaatagt gggctttggc gaaaaatcca attgccagta gaagaaacag
     6181 taacgaagct tttccttttc gtataaacga gccaaaaatg ggcaacaaca atccaaaagc
     6241 aatgacggtt agaatggcgc tgccaaacaa aactttaagc cccaaaccta tcggaaacat
     6301 ttggataaat ggagcaagaa tcagcaaggc cggaatgccc aaaatgaggt tcaaaaccca
     6361 tttcgacttt tgagttaata caaaagaagc caacatcagc aatcccgaga aaacgggaat
     6421 aatcaaaaat cctgcgcctt ttaggaagaa agccagcaat ccattaatga gaatccaaaa
     6481 gaataatggc gcgataaaat ggttcatcgt cacttttttg aaagagaaaa tttggtaaaa
     6541 tgcaaagcaa aaagccaaac tcaacagcgt gaaagccgca atataatcgt gtccgttata
     6601 agtaaatccg ttgagcaaat cgttgtattg tggatagaga atcagcaata atttccatcc
     6661 caaaaaagta actaatccag aaacgattag cgatcccaaa aacggaataa atcctctcgt
     6721 catttcagga aaaaacaaaa tgcgtttgcc aatgccaatg aaaatcaaaa aaagcaagat
     6781 taccaatgcg ataatcacca ttggccaaac ccaactaaat ggataactga tgaaactaaa
     6841 tggaatcgaa aaataaacgt tgtcttcagt ggcttgggtg ctgttcaaat cggcattcga
     6901 aaaataatcc aacaacggca tcaggtattt tccttgatgt gccaaggtat ttttgctcaa
     6961 atgattgagg tcgtcttgcg ccgtgtggta attgtaatgc ccgtcgataa aggcaaaatt
     7021 gtagccttga atattgcctt gttctctgaa aacggtcaaa tcagtatcgt tgggcaacat
     7081 tttgtaaacg ctgtacatca aggaattcga aataggaaat tggacattgg cagccgaaaa
     7141 ttggttcacc aatccggcat ttccttcatt ggtttccatc agcatataac ttgggccgga
     7201 acttcctcgg gcttcaaaat tcaaaaccaa acccacttct tttgcccaat ggtgttgggt
     7261 cacgaaaagt gccgcaccat tcaaacccaa ttcttcggca tcggtaaaca gaatgatgat
     7321 gtcgtttttg tgttgttttt tgttataaac gaaagcgcga atactttcga gaatcgttgc
     7381 cacgcccgaa ccagcatcgc tcgcgccgaa agaataggaa tgcggtgcgc tgtcataatg
     7441 cgaaagcagg agcaaggctt tgttgctgtt gctacctttt attcgagcta gaatattttt
     7501 gcaatgggtc aaattgcccc aatccgaaaa ggaattccct tcttgaatgg aagtttccaa
     7561 acccatattt tgcaattctt tcaccaaata atttgcgatg acatcgtgat tttcggaacc
     7621 tacaaaatgt ggtttttgcg aaatgttttt cacttgtttc aaagctcttt gggtcgaaaa
     7681 ctccgaaagc gaatcatctt cggccgatgt ccattgaggc atcatcgtga agaagacaaa
     7741 tccaaggaaa ccaagaataa ataatacgga taatatagag gaatagtttt ttttcatcgg
     7801 ggatagaatt gggttctaaa tttctttttt gactagcata taatagtcgt cttccaatgc
     7861 gcgatagcct tggtcgtaca cgaaatagta ggtatttcct gttttggtct gcaaaatatt
     7921 ggtaaaaaac tcataatcga atcccttgct caatagtttt gatttggtgg tttttccttt
     7981 tccatccaca tttaatgccg atagaatgcg gtaatttttg cgcaatttgt tgttcacgtt
     8041 gcgcataaaa ttggtgctgt ctttgttaat tttgttgttg taggcattgc gacaaccatc
     8101 gctgcaaaac tttttgtctt cgcgaccaac aattttgtct ccacattcta ggcaggattt
     8161 gtccatagtt ttttattttt aaattctaca cagcaagaag cacttcctga aagagaatct
     8221 ggaagtcact ttcgtcctct gggaggacaa gctcatcggc attgaccttc cgaccaaggt
     8281 ggatctcaag gcgttcgaga ccatgaacgc cgagcgcgga aacacggcg
//
LOCUS       DAOSNN010000101         8273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8609_length_8273_cov_6.510343, whole genome shotgun sequence.
ACCESSION   DAOSNN010000101 DAOSNN010000000
VERSION     DAOSNN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8609_length_8273_cov_6.510343"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..235)
                     /locus_tag="PLQ70_06530"
     tRNA            complement(162..235)
                     /locus_tag="PLQ70_06530"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Asp,seq:gtc)
     gene            complement(321..1247)
                     /locus_tag="PLQ70_06535"
     CDS             complement(321..1247)
                     /locus_tag="PLQ70_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQE34309.1"
                     /translation="MKIESQIEKIASFQHLEMLANQIVEGFISGMHKSPFHGFSAEFA
                     EHKVYNVGESTKHIDWKLFAKTDRLYTKKFEEDTNLRCHIIIDNSSSMHYPKLENPAN
                     FYENKIGFSVLASAVLMNILKKQRDAVGLSVFSDSYEYYSPEKGSDRHHRMILNALEN
                     LLEKPKVQKSTDTIKFLHQIAEKIHRRSMIILFTDMFQSGNEEALFTALQHLKHNKHK
                     VVLFHVIDNKTELNFDFDNAPRKFIDLETGDEVNLFAENVKVEYEKGVNSYFKKLALT
                     CSQNKIKYIPVGVGNNFEKILTTYLVEKQNFG"
     gene            complement(1364..1681)
                     /gene="trxA"
                     /locus_tag="PLQ70_06540"
     CDS             complement(1364..1681)
                     /gene="trxA"
                     /locus_tag="PLQ70_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQE34310.1"
                     /translation="MALAITDATFDEVVLKSDKPVMVDFWAAWCGPCRMVGPIIDQLG
                     EEYEGKVVVGKVDVDANQEFAAKYGVRNIPTVLVFHNGEVVGKQVGVAPKQTYADSLD
                     ALL"
     gene            complement(1792..6369)
                     /gene="dnaE"
                     /locus_tag="PLQ70_06545"
     CDS             complement(1792..6369)
                     /gene="dnaE"
                     /locus_tag="PLQ70_06545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQE34311.1"
                     /translation="MYLIFDTETTGLPKRWGAPISDTDNWPRCIQIAWQLHDEMGKLI
                     EHQDYLVKPEGFNIPYDAERIHGISTELAQAEGILLAEVLEKLNIALGKAKFIVGQNL
                     GFDINIMGCEFYRMGVDSPMSSMPVLDTCTEVTASLLKLPGGRGGKFKLPTLTELHQY
                     LFNKPFAEAHNATADVEATTRCFFELIRTGVFTKQELEVEQEYFEDFQLKNPREIQLI
                     GLQHINLKEASDKIRQKLGGQQTATISKQELSENKETLVDAQFVHLHNHTQFSVLQST
                     ISIPALVKAAAHQKMPAVAMTDHANLMGAFHFVRDILYHNKAAETKNKEAIEKGEEPT
                     EVPMKPIVGCEFFVCEDHKNKSVKDNGYQIVLLAKNKKGYHNLAKMSSIAHTEGFYYV
                     PRIDRKIIQQYKEDIIVLSGNLYGEIPSKILNLGENQAEEALLWWKNEFQDDFYIEVM
                     RHNQEDENRVNEGLIALARKHEVKIVATNNTFYIDKDNANAHDILLCVKEGEKQTTPI
                     GRGRGYRFGLPNQEYYFKSGDEMKQLFNNLPEAILNIAEIVDKIEIYNLAREVLLPKF
                     EIPAEFVDPEDAVDGGVRGENAYLNYLTFEGAKNRYKEITPEIQERLDFELLTIKNSG
                     YPGYFLIVQDFIAEARKMGVSVGPGRGSAAGSVVAYCLKITNIDPLMYNLLFERFLNP
                     DRVSLPDIDIDFDDEGRSSVMDYVIRKYGQKQVAQIITYGKMATKSAIRDTARVLDLP
                     LFEADKIAKLIPGMMPSKWNLARFLSEKEDLIKKTVRPEEFEKIKELIALANEDDLSG
                     ETIQQAKVLEGNLRNTGIHACGVIITPSDITDFVPVATAKDSDLFVTQFDNSVVESAG
                     LLKMDFLGLKTLTLIKDTVKLVKYRSGIELNPDEFPIDDVKTYELFQRGETVGIFQYE
                     SPGMQKYMKELKPTVFPDLIAMNALYRPGPIAYIPSFIKRKNGEEPIVYDLDDCEELL
                     KDTYGITVYQEQVMLLSQKLADFSKGDADVLRKAMGKKMIDVLAKMEPKFISQAMAKG
                     HAKDKLEKIWNDWKAFAEYAFNKSHSTCYAWIAYQTAYLKANYPAEYMAAVLSNNMSD
                     IKQVSFFMEECKRMGLKVLGPDVNESFYKFTVNDDYAVRFGMGAIKGVGSGAVATIVE
                     NRKTGKYKSIFDLTKRIDLRAANKKALENLALAGGFDSFGNTTRAQYFHDDGDGITFY
                     EKAIRYGAKFQENENSSQVSLFGETSDVQIAEPVVPPCEDWSTMEKLAKEKEVVGIYI
                     SGHPLDDFRFEMKYFCPSKLESLKDLSQFVNKSLTFGGIISNVQHRIAKNGKGWALFN
                     LEGYDESYEFKIFGEEYMKYRHYLIPNSFTFIKVMVKEGWPNKETGKKGDPMIQFLEF
                     KMLQDVFDNFAKKLSILLDIKDLNQDFIAKLNAIFQANKGNNSVVFDVVEQEMVQKPI
                     EIVPETIKLESKTDIPLFDDEIPDETVIFSDEFSEDDELIMEESIAESASSLETKVVT
                     RLSMPSRKLKIKISNELLVELENLQINFKLN"
     gene            7021..>8273
                     /gene="leuC"
                     /locus_tag="PLQ70_06550"
     CDS             7021..>8273
                     /gene="leuC"
                     /locus_tag="PLQ70_06550"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQE34312.1"
                     /translation="MSKTLFDKVWDSHVVRKIEDGPDVFFIDRHFIHEVTSPVAFLGL
                     KGRGVKVLYPERTFATADHNTPTINQHLPVQDALSANQLKALEDNANEYGISHWGLGH
                     QKNGIVHVVGPENGITLPGATIVCGDSHTSTHGAFGAIAFGIGTSEVEMVLATQCIMQ
                     PKPKKMRINVNGTLSKGVGPKDVALYIISKLTTSGGTGYFAEYAGNVFEEMSMEGRMT
                     VCNLSIEMGARGGMIAPDQKTFDFLEGRLYAPKGEAWTKAVEYWKTLKTDADAVFDAE
                     LNIDAADIEPMITYGTNPGMGIGISKHIPSAGQVEGGEETYKKSLAYMGFNEDDVMIG
                     KPIDFVFLGSCTNGRIEDFRAFAEIVKGRKKADNVTAWLVPGSHVVEAQIKEEGILDI
                     LTEAGFVLRQPGCSACLAMNDDKVPA"
BASE COUNT         2524 a         1644 c         1408 g         2697 t
ORIGIN      
        1 aaatcaaaaa agctgtccac aaaagtggaa agcttttcat tggtctaact actaaaccgt
       61 gctacgagtt acaaagtcgt agcgaaacaa cacaactatt ttagatgctt ttattgggca
      121 aaaaagcgat tcatttctga atcgcttctt acccaattta gcggtctgga cgggactcga
      181 acccgcgacc ccatgcgtga caggcatgta ttctaaccaa ctgaactacc aaacctctgc
      241 tttattgcgg ttgcaaagat actatagaat ttcgtttcta caagtatttc tgcgaaaaac
      301 attcaatatt tttttgtttt ttatccaaag ttttgttttt caactaaata tgtagtcaat
      361 attttttcga aattattgcc cacaccaacc ggaatgtact taattttgtt ctgagaacag
      421 gtcaaagcta gtttcttaaa gtaactattc acaccttttt cgtattctac cttcacattt
      481 tcggcaaaaa ggtttacctc atcaccagtt tctaggtcaa taaattttct tggagcgttg
      541 tcaaaatcaa aattcaattc ggttttatta tcaattacgt gaaataaaac cactttgtgt
      601 ttgttgtgtt tcaaatgttg taaggctgta aacaaggctt cttcgttacc cgattgaaac
      661 atatcagtaa aaagaataat catcgaacga cgatgaattt tctctgcaat ttgatgcaag
      721 aatttgatcg tatcggtgct tttttgaact ttcggtttct ctaagagatt ttcgagtgca
      781 ttcaaaatca ttcggtgatg acgatcgcta cctttttcgg gcgaataata ttcataggag
      841 tccgaaaaga cgcttaaacc caccgcatca cgctgtttct tgagaatatt catcaaaaca
      901 gccgaagcta aaaccgaaaa tccaatctta ttttcataaa aatttgcggg attttcaagt
      961 tttggatagt gcatcgatga ggaattatcg ataataatgt ggcatcgtaa attggtgtct
     1021 tcctcgaatt ttttggtgta caatctatcg gttttagcaa acaatttcca gtcgatgtgt
     1081 ttggtgcttt cgccaacatt atacacttta tgctcagcaa attcagccga aaaaccgtga
     1141 aaaggacttt tatgcatccc tgaaatgaat ccttccacaa tttgattggc caacatttcg
     1201 agatgctgga aacttgcaat tttttctatt tgagattcaa tcttcattct tcaaatgtat
     1261 taaaaaagaa aagagaaaca agaaaaaaga acacagacat taatggttta aaccaaaaaa
     1321 ggtttgactt tcgccaaacc tttaatataa taaaattgta tgattacaac aaagcgtcta
     1381 agctatcggc ataggtttgc tttggagcaa ctcctacttg ttttcctact acttcaccgt
     1441 tatggaatac caatactgtt gggatgtttc tcactccgta ttttgcagca aattcttgat
     1501 ttgcatcaac atctactttt cctactacaa ctttaccttc atattcttct ccaagttggt
     1561 caatgattgg tccaaccatt ctacaaggtc cacaccaagc tgcccaaaaa tctaccataa
     1621 ctggtttgtc tgatttcaaa accacttcgt caaaagtagc gtctgttatt gctaatgcca
     1681 tatttatatt ttgtttaaag tttaaatttt agagtacaaa gataaagaaa gatttgagtt
     1741 taaaaatgta acatccatta catttagcta tttttaaatt ggtttaattt attaattcaa
     1801 cttaaagtta atctgcaagt tttctaattc taccaaaagt tcattcgaga tcttgatttt
     1861 caattttcga ctcggcattg ataatcgtgt aactactttc gtttctaggc tagaagcgga
     1921 ttcagcaata gattcttcca taatcaattc atcatcttcc gagaactcat ctgaaaaaat
     1981 gacagtttca tctggaattt catcatcgaa taagggaata tcggtttttg attccaactt
     2041 tatcgtttca ggaacaattt ctattggctt ctgtaccatt tcttgctcaa caacatcaaa
     2101 aacaaccgaa ttatttcctt tattggcttg gaaaatggcg tttaatttgg caataaagtc
     2161 ttgattcaaa tcttttatat ccagtaaaat tgacagtttt tttgcaaaat tatcgaacac
     2221 atcctgcaac attttgaatt ccaaaaactg aatcattgga tctccttttt ttccagtttc
     2281 tttatttggc caaccttctt ttaccattac tttaataaat gtaaagctat ttggaatcaa
     2341 ataatggcgg tatttcatat actcttcgcc aaaaattttg aactcataac tttcatcata
     2401 tccttcaaga ttaaatagcg cccagccttt accattcttg gcaattcggt gttgcacatt
     2461 gctaataata cctccaaaag ttaaactttt attcacaaat tgactcaaat ctttgagcga
     2521 ttcgagcttt gaaggacaaa aatatttcat ctcaaatcta aaatcatcca gcggatgtcc
     2581 tgaaatataa attcccacca cttctttttc tttggcgagt ttttccatcg tactccaatc
     2641 ctcgcaaggc ggcacaacag gttctgcaat ttgaacatca cttgtttcgc caaacaaact
     2701 tacctgcgat gaattttcgt tttcctggaa ttttgctcca taacgaatgg ctttttcgta
     2761 aaaagtaatt ccgtcgccat catcatgaaa atattgcgct cttgttgtgt ttccaaacga
     2821 atcgaaacca cccgctaagg ccaagttttc taaagctttt ttgttggccg cccgcaaatc
     2881 aatgcgtttg gtcaaatcaa aaatcgattt gtactttcct gtttttctgt tttcgacaat
     2941 ggttgccacc gctccagaac ccactccttt tatggctccc atcccaaaac gaaccgcata
     3001 atcatcattt acggtgaatt tataaaacga ttcgttcacg tctggcccca aaactttcaa
     3061 tcccattcgc ttacattctt ccataaaaaa agaaacttgc ttgatatcgc tcatattatt
     3121 ggacaaaacc gctgccatat attcggctgg ataattggct ttcaaatagg ccgtttggta
     3181 ggcaatccaa gcataacaag tagaatgcga tttgttgaaa gcatattcgg caaaggcttt
     3241 ccagtcattc caaattttct ctaatttgtc tttggcatga ccttttgcca tggcttgcga
     3301 aatgaattta ggttccattt tggccaaaac atcaatcatc tttttcccca tcgctttacg
     3361 caaaacgtcg gcatcacctt tggagaaatc cgccagtttt tgggacaaaa gcattacttg
     3421 ctcttgataa acggtaattc cgtaggtgtc ttttagtaat tcttcgcagt catccaagtc
     3481 atacacaatg ggttcttcac cgtttttacg tttaataaaa cttggaatat aagcaattgg
     3541 tcccggacga tacaaggcat tcatggcaat caaatcagga aaaaccgtag gctttaattc
     3601 cttcatgtat ttttgcattc cgggactttc atattgaaaa ataccaaccg tttctcctcg
     3661 ttgaaaaagt tcatacgttt tgacatcatc aataggaaat tcgtcgggat tgagttcgat
     3721 tccgcttcga tattttacta atttaacggt gtcttttatt aaagttaggg ttttcaaacc
     3781 caagaagtcc atcttcaaca aacccgcact ttccacgacc gagttgtcga attgagtgac
     3841 aaacaaatcc gaatctttgg ccgtagcaac tggaacaaaa tcagtaatat cgcttggcgt
     3901 aataatcaca ccacaagcgt gaatacctgt atttcttaga tttccttcta atactttggc
     3961 ctgttgaatg gtttctccac tcaaatcatc ttcattggcc aaagcgatta attcttttat
     4021 tttttcgaat tcttcaggac gaacagtctt tttgataaga tcttcttttt cactcaaaaa
     4081 ccgagctaaa ttccatttag aaggcatcat tccaggaatt aatttagcaa ttttatcggc
     4141 ttcgaataac ggcaaatcca atacacgagc cgtatcacga atagccgatt tggttgccat
     4201 ttttccgtag gtgataattt gagctacttg tttctgtccg tactttcgaa tcacataatc
     4261 cattacgcta ctgcggcctt cgtcatcaaa atcaatatca atatcgggaa gagaaaccct
     4321 atcaggattc agaaaacgct caaaaagcag attgtacatc aaggggtcga tattggtgat
     4381 tttcaaacaa taagccacca ccgaacctgc cgccgaaccc cttccaggtc caacagaaac
     4441 ccccattttt cgagcttcgg caatgaaatc ctgcacaatc aaaaagtatc ctggatatcc
     4501 tgaatttttt attgtaagca attcgaaatc taatctttct tgaatttcgg gcgtaatttc
     4561 cttgtatcga ttctttgccc cttcgaaagt gagataattc aaataggcat tttcgcctcg
     4621 aactcctcca tcaacagcgt cttctggatc gacaaactcg gctggaattt caaactttgg
     4681 aagcaaaact tcacgagcca aattgtaaat ttctatctta tcaacaatct ctgcaatgtt
     4741 caaaatagct tcaggcaaat tattgaacaa ttgcttcatt tcatcgcccg acttgaaata
     4801 atattcctga ttgggcaatc caaaacgata gcctcgaccc cgaccaattg gagtggtttg
     4861 cttctcccct tcttttacac acaataaaat gtcgtgcgca ttggcattgt ctttatcaat
     4921 ataaaaagta ttattggtcg ccacaatttt gacctcgtgc tttcttgcca aggcaatcaa
     4981 gccctcgttc acacggtttt catcttcttg attgtgacgc atcacttcaa tataaaaatc
     5041 gtcctgaaac tcgtttttcc accaaagcaa agcttcttcg gcttggtttt cgcccagatt
     5101 taaaatctta ctcggaatct ccccatatag atttcccgat aaaacaataa tatcttcctt
     5161 atactgctga ataatttttc ggtctattct tggtacataa taaaaccctt cagtgtgtgc
     5221 aatagaagac attttggcca aattatgata gccttttttg ttctttgcca gcaacacaat
     5281 ttgatatcca ttgtctttta ccgacttatt tttgtggtct tcacaaacaa aaaattcgca
     5341 acccacaata ggtttcatcg gaacctcggt tggttcttcg cctttttcaa tggcttcttt
     5401 gtttttggtt tcagccgctt tgttgtggta taaaatgtca cgaacaaagt gaaatgctcc
     5461 catcaaattg gcatgatccg tcatcgccac agcaggcatt ttttgatgtg cagctgcttt
     5521 aaccaaggct ggaatactaa ttgtggattg caaaaccgaa aactgagtat gattgtgcaa
     5581 atgcacgaat tgagcatcga ctaaagtttc tttattctct gaaagctctt gtttagaaat
     5641 ggttgctgtt tgctgaccgc ctaatttctg acgaatttta tcggatgctt cctttaaatt
     5701 gatgtgctgc aatccgatga gttgaatttc tctaggattt ttgagttgaa aatcctcgaa
     5761 atattcctgc tcgacttcga gttcctgttt agtgaaaact ccggtacgaa tcaattcgaa
     5821 aaaacaacgc gtggttgcct caacatcggc agtggcattg tgcgcttccg caaaaggttt
     5881 attgaaaagg tattggtgta attcggtaag tgtaggtaat ttgaacttac cgccacgacc
     5941 tccgggtaat ttcaataaag aggcagtaac ttcagtacaa gtatctaaaa ccggcatcga
     6001 actcattgga gaatcgactc ccattctgta aaattcgcat cccataatat tgatatcaaa
     6061 acctagattt tgacccacaa taaatttagc tttacctaaa gctatgttga gtttctccaa
     6121 aacttcggcc agcagaattc cttcggcttg cgccaattca gtggaaatac cgtggattct
     6181 ctcggcatca tacggaatat tgaacccttc tggtttgacc aaataatcct gatgctcgat
     6241 gagttttccc atctcgtcgt ggagctgcca agcaatttga atacatcttg gccagttgtc
     6301 cgtgtcggaa atgggagcgc cccaacgctt gggtaaacct gtggtttcgg tatcgaatat
     6361 taagtacata gctggatttt aatgctttgc aattgcctga aattacattt agcagtttcc
     6421 aaatttagaa ttttttagac gaaaaaagca tgtaagttat caacaataaa ttcctttaaa
     6481 atcaaaaata gaattgtttt ctttaaacca tttctgcaaa ttatttattc caaaattcac
     6541 aaaaaaactc aaaattccat ttcttggttg tcgtaatatt ttataaaaaa aattgctttt
     6601 aaatacgact taataattct aatttcagca gccaaaaagc aaaaaatgta ttttattttg
     6661 caataaacag aaaggcgaaa ctttaaaaat tattactatt tttattcgtt ttgttacatt
     6721 ttatagatat aaatctgctt tttaatgcta aaatagctgt aattttgcaa aaatttaaaa
     6781 aacagcatta gttgattaaa acaagttcaa aaagtgaatt aaagtcaatt taaaaaacga
     6841 tttaaaaacg attttctata tcagaagtag taagtagtta taaaaacaac tttgggtatc
     6901 gtatataaac aaatagtagg attgacacaa gcaattcttt ttataaattt gctccactat
     6961 tttaatttgg gttaattgcc aaaattttaa aaagagaaat acactaaaaa aatatttaaa
     7021 atgagtaaga cattatttga caaagtatgg gattcgcacg tagtacgaaa aattgaagat
     7081 ggaccagacg tattttttat tgaccgtcat ttcattcacg aagttactag tcctgttgct
     7141 tttcttggtt taaaaggaag aggagtaaag gttttgtatc ccgagcgtac ttttgccact
     7201 gccgaccaca acacaccaac tataaaccaa catttgcctg ttcaggatgc tttatctgca
     7261 aatcagctaa aagcattaga agataacgcc aatgaatacg gtatttctca ctggggatta
     7321 ggacaccaaa aaaatggtat cgttcacgta gtagggcctg aaaacggaat tactttgcca
     7381 ggtgccacta ttgtttgtgg cgattcgcac acttctactc acggtgcttt tggagcgatt
     7441 gcttttggta tcggaacatc tgaagtggaa atggttttgg ctactcaatg tattatgcag
     7501 cctaagccaa agaaaatgcg tatcaacgta aacggaactt tgagcaaagg tgttggccca
     7561 aaagatgtag ctttgtacat catttcaaaa ttaaccactt ctggaggaac aggatatttt
     7621 gctgaatatg ccggaaatgt tttcgaagaa atgtctatgg aaggtcgtat gacggtttgt
     7681 aacctttcta tcgaaatggg tgctcgtggt ggtatgattg ctcctgacca aaaaactttc
     7741 gatttcctgg aaggacgttt atatgctcca aaaggagaag cttggacaaa agcggttgaa
     7801 tattggaaaa ctttaaaaac cgatgctgat gccgttttcg atgctgaatt gaatatcgat
     7861 gctgcagata tcgaaccaat gattacttat ggtacgaatc ctggaatggg aattggtatc
     7921 tcaaaacata ttccaagtgc tggccaagta gaaggtggcg aggaaactta caaaaaatct
     7981 ttggcttata tgggcttcaa cgaagatgat gtgatgattg gaaaaccaat tgatttcgtt
     8041 ttcttaggaa gttgtacaaa cggacgtatt gaagatttta gagcttttgc tgaaattgta
     8101 aaaggtcgta aaaaagcaga caatgtaaca gcttggttag ttccaggttc acacgttgtt
     8161 gaagcacaaa tcaaagaaga aggaatttta gatatcctta ctgaagcagg tttcgtgttg
     8221 cgtcagccag gttgttcagc ttgtttggca atgaacgatg ataaagttcc tgc
//
LOCUS       DAOSNN010000102         8272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8610_length_8272_cov_4.458075, whole genome shotgun sequence.
ACCESSION   DAOSNN010000102 DAOSNN010000000
VERSION     DAOSNN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8610_length_8272_cov_4.458075"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLQ70_06555"
     CDS             <1..219
                     /locus_tag="PLQ70_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34313.1"
                     /translation="VIDPGQVVELGKTYEFKIISIEPDIHRLGLSLKALSGKNKEEKK
                     EKEEETVETEAKTESKKVKKETKKSVKK"
     gene            727..1725
                     /locus_tag="PLQ70_06560"
     CDS             727..1725
                     /locus_tag="PLQ70_06560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE34314.1"
                     /translation="MLFSLIIPVYNRPDEVDELLESLSQQDYTDNFEVVIVEDGSELR
                     CDDVVRKYAGKLHLSYSFKGNSGPGDSRNYGMKKANGDYFIIFDSDCIIPKSYLTEVA
                     KALKENYVDCFGGPDAALDSFSDIQKAINFAMTSFLTTGGIRGGSEKIDKFQPRSFNM
                     GLSRKAFEASNGYGNIHPGEDPDLSIRLWNLGFETQLFPKAFVYHKRRIDWEKFTIQV
                     NKFGKARPILNSWYPQYNKLTFFFPSFFIIGLLFAVVLLIFTYDVLLQLYFVYFLMIF
                     LVSTYKNKSFKVGLLSIKAVWKQFFGYGTGFIESFIKVIILNQKPQEAFPELFFKK"
     gene            1727..2308
                     /gene="coaE"
                     /locus_tag="PLQ70_06565"
     CDS             1727..2308
                     /gene="coaE"
                     /locus_tag="PLQ70_06565"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807924.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQE34315.1"
                     /translation="MTKIIGLTGGIGSGKTTIANLFREANIPVYIADDEARKIMQSPE
                     IIEEIKNTFGSEIFDDTILNREKLSQIVFNEPEKLKLLNAIIHPAVRKHFQNWVLNHE
                     KYPFVIYEAAILFESGSYKNCDLIITVTAPIETRIERVIQRDKTTREQVLKRINAQWD
                     DTQRISKSDFVIVNIDPKTTKLEIGKILKILNI"
     gene            2383..3966
                     /locus_tag="PLQ70_06570"
     CDS             2383..3966
                     /locus_tag="PLQ70_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQE34316.1"
                     /translation="MNKLFFRILVLLMSLSLIGIILVQVYWFNTSLENNDEQFKFHVK
                     SVIGNVAEKLQKQEEYTYYEKFNRYKDSTGKIPQKDDLLEFYYVQKNHKNNTTIVFTN
                     SISSEDYTISSSFFDKKSDSLKLKSFNSKRVTEIYNNNKFDNSKLQNTVIPDVKNEKS
                     GSLDVLDDIAYEINYKEIASAMPLQERVSKETIQKLLKKELEEYGVNTKFEFGVFSSS
                     LATKVKSDGFIYDSKNTYSIPIFSDNEGSNKYELMVSFPNKKKFLLTDLIGITLLSII
                     FTLIIIIAYTSALNQLIRQRQISEIKSDFINNMTHEFKTPIATINLALDAIKNPKIIG
                     NEQMVLKYLQMIKDENKRMHAQVENVLRISKLEKKELDISKESVNIQEVIEDAMEHVN
                     LILEDRQGTIDTHFNAERTSVLLNEVHFINVIVNILENAIKYSPDIPKIDIYTENVKD
                     YLIIKVKDNGIGMSKVAQKRVFEKFYREHTGDLHDVKGHGLGLAYVKKIIDDHNGQIY
                     VESEKGKGSTFIIKIPLIN"
     gene            3970..4674
                     /locus_tag="PLQ70_06575"
     CDS             3970..4674
                     /locus_tag="PLQ70_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQE34317.1"
                     /translation="MENVNKRILLVEDDLNFGAVLKDYLMLNDFDVTLAKNGMEGFEK
                     FKKDNYDLCILDVMMPYKDGYTLAKEIREKNQEVPIIFLTAKSMKEDVLKGYKAGADD
                     YLNKPFDSEVLLMKIRAIIQRKSSTVQAEPVQFEFNVGKFHLNSKLRFLSIGDEEPVK
                     LSPKENELLKMLILHENDLMPRELALTKIWRDDNYFTSRSMDVYIAKLRKYLKLDSNV
                     EILNIHGEGFRLVVKK"
     gene            complement(4748..7294)
                     /locus_tag="PLQ70_06580"
     CDS             complement(4748..7294)
                     /locus_tag="PLQ70_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34318.1"
                     /translation="MKIKLRIIVFTVLFLFFNTTMLLAQKHQVSNNKDLIFNGKHIIY
                     KGNKIELDPKSFYIDGQLSNEEAAKHPYVYNSINEASKHLTNGTEASPMVLYIAPWVY
                     WIDNPNDPEIRVPKTIGGTPFGLEINCEWLRFQGLSDNAQDVVLACNRGQTIGSKGNF
                     TMLNIKGEGISSENITFGNYCNIDLIYPLNPKLNREKRSSAIVQGQLIFSNGDKVFAR
                     NTCFVSRLNLGPFWGSKRTLFDRCHFESTDDALNGTAVYLNCSFDFYSSKPFGHTSGT
                     GAVFLNCDIQSFTRERQYFVKGSGPVAAIDTRFTAQNLDYIGWREIPDTEARYYQYNI
                     SLNAKPIGLGKDFPNITVDLTGKELLNAYRFEFNNATVYNTYNLLRGNDNWDPMGIKS
                     IVEKAEKESGKNYSSIPTLLKIKPSRETLETGKDSVTLEATVNRFGNYELKDQKVVWK
                     IAPEYQSLVKLEVNEDGKCVVIPINKGNETKEVIITASTESGLEAASVFQIAPAFMAP
                     PTFTTLPRINKTTDGKLLLNYALDMTFEDESLVNWYRCRDVKGSHPIEIAVSRNNKPL
                     KTYDLSSGDIGYYIMASISPKHLRCQAGTPKTVVFKDRISKKDIKESTILVPNFENMS
                     AKFQPEILPGFWTLDSFAPADTNDQNWVADNSISPWFYGKGENGAANDVGFIQASKGA
                     RMRYTPVGNSFGDMKISFTAVPAKTAGQGFSSARSQYMDIGIKMDTKKINGYALRIIR
                     TTKFSDAVDFILMKYENGVAIPISKSVTTSCYRPNCVITIEVKNKKISIQAKNTLDYF
                     AENYPAEVMKTVNLEAEIIPNTFGGISFQHTGTIGSGATLIKDLQIEWKP"
     gene            complement(7319..8059)
                     /locus_tag="PLQ70_06585"
     CDS             complement(7319..8059)
                     /locus_tag="PLQ70_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HQE34319.1"
                     /translation="MPISPYFTSIFSKDWEINFSQCLPNGSLKYTDLCNILQLTAAAH
                     SEVGGISFSDMQEFNQAWVLSRMRVEITELPQWKDVVTLKTWINTLENSRSVRALELY
                     VNGKKMVGCETFWAVFNTKIRRPEALALPFEHFELYPEQKATEKPFSKINFSSETEMV
                     FEKTVALSDLDIVNHVNNVKYLEWCLDLVDTELILNQKIESFEMNFLKELSLKDQVAI
                     HENLTSEAMVFSITKDNTTCFALQLNLK"
BASE COUNT         2789 a         1256 c         1493 g         2734 t
ORIGIN      
        1 gtgattgatc ccggacaggt tgttgagttg ggcaaaacct acgaatttaa aataatctcc
       61 atcgaaccag atattcatcg tctgggctta agtctcaaag cgctttctgg caaaaataag
      121 gaagaaaaaa aagagaaaga agaggagact gttgagacag aagctaaaac agagagcaaa
      181 aaggtgaaga aagaaacaaa aaagagtgtt aaaaaataag tgagccgggt agtgaaaatt
      241 cgacagcttt taaaaaatta gccttcgata tgctctgccc gtagcgaaac actttaaaat
      301 ctaatttatt taatggctct gaaaatttaa gctccaaact attttttttt ccgcaactaa
      361 aaagagtcat aaaaagtaga aaaatcaatt gggttatttt tttcataatg aattagtatt
      421 tttcccgatt gctcggataa agaaaatatt tctctacgga tagcacaaat tttattctgt
      481 atctattgta ataaactact taaacaaatt taataaaatt atagccagct ttctttttta
      541 atggcttgtt tttactgtga taaattaaaa aatcattgtt gaaaataaat gattatctgt
      601 cataaaatga agtacaaaaa agccaaatca attatctaaa atttgcgttt aagtattgaa
      661 tccgccattg ataaagtata tctttgcaaa aaataaatac tatttcataa attttaattc
      721 aagagtatgt tgttttcttt aatcataccg gtttacaatc gcccagacga agtggatgaa
      781 cttttagaga gtttgtcaca acaggattat acagataatt ttgaagtggt aatagtagaa
      841 gatggatctg agttgcgatg cgatgatgtg gtgcgtaaat atgctggaaa attgcatttg
      901 tcctattctt ttaaaggaaa ttcaggtcct ggtgattctc gaaattatgg aatgaaaaag
      961 gcaaatggcg attattttat catttttgat tcagattgta ttattccaaa aagctatttg
     1021 actgaagttg ccaaggcgtt aaaagaaaat tatgtcgatt gttttggtgg acctgatgcg
     1081 gctttagata gtttttcgga tattcaaaaa gcaatcaatt ttgcgatgac ttcttttctt
     1141 actactggcg gaattcgtgg tggttcggag aagattgata agtttcaacc ccgaagtttc
     1201 aatatgggat tgtctcgaaa agcttttgaa gcatctaatg gttatggaaa tatacaccca
     1261 ggcgaagacc cagatttatc tataagattg tggaatttag gttttgaaac ccagcttttt
     1321 cctaaagcat tcgtgtatca caaacgaaga atcgattggg aaaaatttac tattcaagtc
     1381 aataaatttg gcaaagccag gccaattctc aatagttggt atcctcaata caacaaactt
     1441 acttttttct ttccctcatt ttttataatt ggactacttt ttgctgtggt tttgttgatt
     1501 tttacttatg atgtattgct tcaattgtat tttgtttact ttttaatgat ttttttggtt
     1561 tctacttata aaaataaaag tttcaaagtg ggtttgttat ctatcaaagc ggtttggaaa
     1621 caattttttg gctatggaac agggttcatc gaatccttca ttaaagttat tatattgaat
     1681 caaaaaccgc aagaggcttt tcctgagttg ttttttaaaa aataaaatga caaaaataat
     1741 tggattaact ggaggtattg gaagtgggaa aaccactatc gctaatcttt ttagggaggc
     1801 taatattcca gtttatattg ctgatgacga ggcaagaaaa attatgcaat cgcctgaaat
     1861 tattgaagaa atcaaaaata cttttggttc tgaaattttt gatgatacta ttctaaacag
     1921 agaaaaacta tcacaaattg tatttaatga gcccgaaaaa ttaaaacttc tgaacgcaat
     1981 tatacaccca gcggttagaa aacatttcca gaattgggtt ttaaatcacg agaaatatcc
     2041 gtttgttatt tatgaagccg ccattttgtt tgagagtggc agttataaaa attgtgattt
     2101 aataataaca gtgactgcac caatcgaaac cagaattgag cgtgtcattc agcgcgacaa
     2161 aactactcgg gagcaagttt taaagcggat taatgcacaa tgggatgata ctcaacgtat
     2221 ttctaaaagt gattttgtta tagtaaacat tgatccaaag accacaaaat tagaaatagg
     2281 taaaattctt aaaattttga atatttgaca atcagaatct ttgttgttaa tctttggtta
     2341 atttattatt agttatactg ttaaaatact aaatttgtat cgatgaataa attatttttt
     2401 agaatactgg ttttattgat gagtttatcc ttgatcggga taattttagt tcaagtatat
     2461 tggtttaata cctcgttaga aaataatgac gagcaattta aatttcatgt aaaatcagta
     2521 ataggaaatg ttgccgaaaa gcttcaaaaa caagaagaat atacctatta cgagaaattt
     2581 aatcggtata aagacagcac gggaaaaatt cctcaaaagg atgatttatt agagttttat
     2641 tacgttcaaa aaaatcataa aaataatacc actattgttt ttacaaacag tatttcatct
     2701 gaagattata caatttcttc atcgttcttt gataaaaaat cagatagttt aaagttgaaa
     2761 agctttaatt ctaagagggt tacggaaata tataacaata ataaatttga taattcaaaa
     2821 ttgcaaaata cggtaatacc agatgtaaag aatgaaaaat cggggagttt ggatgtattg
     2881 gatgatatag cttatgaaat taattacaaa gaaattgctt ctgcaatgcc gcttcaagaa
     2941 agggtttcaa aagaaacaat tcagaagtta ttgaaaaaag aattagagga atatggtgta
     3001 aatacaaaat ttgaatttgg tgtttttagc agtagtttag cgactaaagt aaaatcggat
     3061 ggatttattt atgattcaaa aaacacctat tctataccta ttttttcaga taatgaggga
     3121 agtaacaaat atgaattaat ggtatctttt cccaataaaa agaaattttt gcttactgat
     3181 ttgattggaa tcacattatt atcaatcatt tttacgctca taattattat tgcttacaca
     3241 agtgcactga accaattgat tcgtcagcgt caaatatcgg agataaaatc agatttcatc
     3301 aacaatatga cacacgaatt caaaacacca attgcaacta taaatttggc tttagatgca
     3361 attaaaaatc caaaaataat tggtaatgag cagatggttt tgaagtacct tcaaatgatt
     3421 aaagacgaaa acaagagaat gcatgctcaa gttgaaaatg tgttgagaat ttcaaaatta
     3481 gaaaagaaag agttggatat tagtaaggaa tctgttaata ttcaagaggt tattgaagat
     3541 gctatggagc atgtcaattt gattttagaa gacagacaag gtacaattga tacacatttt
     3601 aacgctgaga gaacttcagt tttattgaat gaagttcatt ttataaacgt aattgtgaat
     3661 attttggaaa acgctatcaa gtattctccg gatattccta aaattgacat ttataccgaa
     3721 aatgtaaaag attatttgat tattaaagtc aaagacaatg gcataggaat gagtaaagtg
     3781 gctcagaaaa gagtttttga gaaattttat cgagaacata ctggagattt gcacgatgtt
     3841 aaaggtcatg gtttaggatt ggcttatgtt aaaaagatta tagacgacca caacggtcaa
     3901 atttatgtag aaagtgaaaa gggaaaagga agtaccttca taataaaaat accattaata
     3961 aattaagata tggaaaatgt aaataaaaga attcttttgg ttgaggacga ccttaatttt
     4021 ggagctgtgt tgaaagatta tttaatgcta aatgattttg atgttactct ggccaaaaac
     4081 ggaatggaag gctttgagaa attcaaaaaa gataattacg atttatgtat tttggatgta
     4141 atgatgcctt acaaggacgg ctatacttta gcaaaagaaa ttagagaaaa aaaccaagaa
     4201 gtgccaataa tattcttaac cgcaaaatct atgaaagaag atgtgctaaa aggatacaaa
     4261 gctggagccg atgattattt gaacaaaccc tttgattcag aggttttgtt gatgaaaatc
     4321 agagctatca ttcaaagaaa atcatctaca gttcaagctg aacctgttca atttgaattt
     4381 aatgtaggta aatttcattt gaattccaaa cttcgatttt tgtctattgg cgatgaagaa
     4441 ccagtgaaat tgtctccaaa agaaaacgaa ttattgaaaa tgttgattct gcatgagaac
     4501 gatttgatgc caagagaatt agcattgact aaaatttgga gagatgataa ctatttcact
     4561 tccagaagta tggatgttta tatcgctaaa ttaagaaagt atcttaaatt agattcaaat
     4621 gtagaaatct tgaacattca cggagaaggt tttagattag tagttaagaa ataaaacatt
     4681 ttgtttgaaa tataaaaagg cttcgagaaa ttcgaagcct ttttggttgt tgatagttta
     4741 gaaaatttta tggtttccat tctatttgta aatcttttat caaagtggct ccgcttccaa
     4801 tggttcctgt gtgttgaaaa ctaattcctc caaaagtatt tggaatgatt tcggcttcga
     4861 gatttacagt tttcataact tcagctggat aattttcagc aaaataatcg agagtatttt
     4921 tagcctgaat actaattttt ttgtttttta cttcaatggt aatgacacaa ttaggacgat
     4981 aacaagatgt ggtcacagat ttgctaatag gaattgcaac cccgttttcg tatttcatta
     5041 gaataaaatc gacagcatcg ctgaattttg tggtgcggat gattcggagc gcatagccgt
     5101 ttattttttt agtatccatc ttgattccaa tgtccatata ttgacttctg gcactgctaa
     5161 atccttgtcc agccgttttg gcaggtacag ccgtgaagct aattttcata tctccaaaag
     5221 aatttccaac cggagtataa cgcattctag cccctttgga agcctgaata aatcccacat
     5281 cattagcggc tccattttcc cctttgccat aaaaccaagg cgaaatactg ttgtctgcaa
     5341 cccaattttg gtcatttgtg tcggctggag cgaaactgtc aagagtccaa aatccgggta
     5401 gaatttcggg ttggaattta gccgacatat tttcaaaatt tggaacaaga atagtagatt
     5461 ctttgatgtc tttttttgaa atcctatctt tgaacactac tgtttttggc gttcctgctt
     5521 ggcatcgcaa atgttttgga gaaatgcttg ccataatata ataaccaata tctccagatg
     5581 aaagatcgta ggttttcaag ggtttattat ttctagaaac ggcaatttct atgggatgac
     5641 taccttttac atcacgacat cggtaccaat taaccaacga ttcatcttca aaagtcatat
     5701 ctaaggcgta gttcagcaat aattttccgt cggtggtttt attaattctt ggaagtgttg
     5761 tgaatgttgg tggagccata aaagcaggcg caatttggaa tacgctggct gcttccaatc
     5821 ctgattcggt tgaagctgtg ataatcactt cctttgtttc attgcccttg tttataggaa
     5881 tgacgacaca tttcccgtct tcattcactt ctaatttgac caaggattgg tattcaggag
     5941 ctattttcca aactactttt tggtctttta attcataatt tccaaaacga ttcactgtgg
     6001 cttccaatgt tacgctgtcc tttccagttt ccaaagtttc cctggaaggc tttattttca
     6061 gtagtgttgg aatagaggag tagttcttac cagattcttt ttcagctttt tctacaatac
     6121 ttttaattcc cattggatcc caattgtcgt tgcctcgcaa taaattgtag gtattataga
     6181 cagtggcatt gttgaattca aagcggtaag cattgagtaa ttcttttcct gtcaagtcaa
     6241 cggttatatt ggggaaatct ttgccaaggc caatcggttt ggcatttaag gaaatgttgt
     6301 actgatagta acgcgcttcg gtatcgggaa tttctcgcca gccaatataa tctagatttt
     6361 gggctgtaaa tcgggtgtcg atggcagcca ctggaccact tccttttacg aaatactgtc
     6421 tttcacgagt aaaggattga atgtcgcaat tcagaaaaac ggcacctgtt cccgaagtgt
     6481 gaccaaaagg tttcgaggaa taaaaatcaa agctgcaatt aaggtaaacc gccgttccgt
     6541 tcaaggcgtc gtcggtcgat tcgaaatggc aacgatcaaa aagggttctt ttacttcccc
     6601 aaaacggtcc caaattaagt ctgctcacaa aacaagtatt tcttgcaaat actttatctc
     6661 cattcgaaaa aatgagttgc ccttgcacga tggccgagct tcttttttct cggttcagtt
     6721 ttggattcaa aggataaatc aggtcaatgt tgcaataatt tccaaaagta atattctcgg
     6781 aacttatacc ttctcctttg atgtttagca ttgtgaaatt acctttggat ccgatggttt
     6841 gtccacgatt gcaagctaaa actacatctt gcgcattatc ggacaaccct tgaaaacgca
     6901 accattcaca attaatttcg agtccaaatg gcgttccacc aatagttttt ggaacacgga
     6961 tttcgggatc gttcggattg tcaatccaat atacccaagg cgcaatgtac aaaaccattg
     7021 gcgaggcttc ggttccgtta gtcaaatgtt tggaggcttc gtttattgag ttgtaaacat
     7081 aggggtgttt agccgcttct tcattcgaaa gttgtccgtc aatataaaaa gattttggat
     7141 caagctcaat tttattgcct ttataaataa tatgtttacc attgaaaatc aagtctttat
     7201 tgttggaaac ttgatgtttt tgagcaagta gcattgtagt attaaaaaac aaaaataaga
     7261 cagtaaaaac aattattcga agcttgattt tcataaagat ataaatttaa aaggtcattt
     7321 atttcaaatt caattgcaag gcaaagcagg ttgtattatc tttggtaata ctaaaaacca
     7381 ttgcttccga agttaggttt tcgtgaatag cgacttggtc ttttaatgac aattctttta
     7441 gaaaattcat ttcaaaactt tcaatttttt ggtttagaat caattctgta tccaccaaat
     7501 ccaaacacca ttccaaatat tttacattat ttacgtgatt gacaatgtct aaatccgaaa
     7561 gtgccacggt tttttcgaag accatttcgg tttcgctact gaagttgatt ttggaaaaag
     7621 gtttttcggt tgctttttgt tccggataca attcaaaatg ttcgaaaggc aaagccaaag
     7681 cttctggtcg tctaattttg gtattgaaaa ccgcccaaaa agtttcgcaa cccaccattt
     7741 tttttccgtt gacatacaat tccaaagcac gaactgaacg ggaattttcg agcgtgttaa
     7801 tccaggtttt taacgtcacg acgtctttcc attggggtaa ttccgttatt tcaacccgca
     7861 ttctgctcaa aacccatgcc tgattgaatt cctgcatatc cgaaaaactg atgccgccca
     7921 cttcagaatg tgccgcagcg gtcaactgca aaatattgca caaatcggtg tattttaaag
     7981 aaccattggg aagacattgg gagaaattaa tttcccaatc tttgctaaaa atggacgtga
     8041 agtagggtga aatgggcatt tttatattat gaattaatgt ttttgttgaa ataaggtttg
     8101 aacattttaa tatttaaata aataaaaaac aaaaggatta tacccaaaaa aaggtacttg
     8161 aattatccaa atttttaaaa tttggctttt ctattttcgt ttctattact atcaaaaatg
     8221 ggactaataa atatataata gtaaaaagta aaaaaaatat tttacctatt aa
//
LOCUS       DAOSNN010000103         8265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8614_length_8265_cov_4.987089, whole genome shotgun sequence.
ACCESSION   DAOSNN010000103 DAOSNN010000000
VERSION     DAOSNN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8614_length_8265_cov_4.987089"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /gene="lysS"
                     /locus_tag="PLQ70_06590"
     CDS             <1..1387
                     /gene="lysS"
                     /locus_tag="PLQ70_06590"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQE34320.1"
                     /translation="DFIGIEGELFTTKVGAQCIRVDKFTFLSKTLRPLPLPKVDEDGK
                     VHDAFNDAELRYRMRYVDLTVNQNVKDTFIKRTKLFTAMRGYFNDAGYLEVDTPVLQS
                     IPGGASARPFITHHNSLDIPLYMRIANELYLKRLIVGGFEGVYEFSRNFRNEGMDRTH
                     NPEFTAMEIYVAYKDYNWMMEFAEGLLEHCAIAVNGTSEVTFGEHQINFKAPYARVTM
                     TDSIKHFTGFDISGKTEEELFAAAKGMGIEVDETMGKGKLIDEIFGAKCEGNYIQPTF
                     ITDYPKEMSPLCKEHRDNPDLTERFELMVCGKEIANAYSELNDPIDQRERFEDQMRLA
                     AKGDDEANGIIDEDFLRALEYGMPPTSGMGIGMDRLIMYLTNNASIQEVLLFPQMRPE
                     KKQTIELSEEEKLIVALLKANENKMDLAQLKVTAALSGKKWDAATKGLSKNNLIKVSV
                     DGESKVMELVE"
     gene            complement(1670..2275)
                     /locus_tag="PLQ70_06595"
     CDS             complement(1670..2275)
                     /locus_tag="PLQ70_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HQE34321.1"
                     /translation="MARRPDNTFKTRAFRSKVLILCEGMTEKNYFTAMKEDFRLPKTI
                     GVAIFQSNKVDCKGVVEEAIKKAKREGFQEIFVVFDHDNQPKRDEAFKLASNKKIKVI
                     FSSICFESWYLLHFKNSTKAFASEAELEKELKKCEGMKNYEKNDFKHYSTLKDKLAFA
                     KSNAENVRLFILENNYDVAVFNLNPYTNVDELVLFLEKQRT"
     gene            complement(2278..3444)
                     /locus_tag="PLQ70_06600"
     CDS             complement(2278..3444)
                     /locus_tag="PLQ70_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQE34322.1"
                     /translation="MIPSDFKNPVNRIIKTNPNATKNKDYYGLTSAAIYGPNASGKSN
                     IVKALGAFRNFVKNSTDNKPNDTIKLYNPFILCKGNVLKPSCFSIDFLINNVLHIYSI
                     EVFQKEIIAESLFYFPKGLKVKVFERKKQEFSKGNGYEGIFETIKQRTTENQLFLGKA
                     AIENVEIAKSVYESITSMLVLTTDLFHSEEFNKTASKLFFTDLNDGGVILRLSKSLIQ
                     KLDTQIVNFEEKEGVIKTFHFNDNFEQIEFDFEDESSGTQRIMSFVPLVFIALSSGST
                     IIVDEFERSLHPEIAQYILGLFNDPEVNTKGAQFIFATHDTTLLSPENKLRRDQINLV
                     EKNVKGETEMFAVSDLKGIREGNYEKWYLEGRLGAVPNIARETFRYELIDFINS"
     gene            complement(3652..4134)
                     /locus_tag="PLQ70_06605"
     CDS             complement(3652..4134)
                     /locus_tag="PLQ70_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQE34323.1"
                     /translation="MKKNNNFEIDKPIKRSESLKPLSREHHHGLLFCWKIRAGIEKSV
                     DISRIKKYADWFYQNYLIPHFEVEEKYVFPILGNENELIKKAVSEHDRIKQLFESNTE
                     ISKNISLIEEELESHIRFEERILFGEIQKITTAEQLQSIEINHSDEKFVDNLTDPFWE
                     "
     gene            complement(4185..4907)
                     /gene="ric"
                     /locus_tag="PLQ70_06610"
     CDS             complement(4185..4907)
                     /gene="ric"
                     /locus_tag="PLQ70_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HQE34324.1"
                     /translation="MNIQENQIIGELVAKDYRTASVFKKYGIDFCCQGNRTINEACEK
                     KNIDSKSVVNDLDAIIQAQGENTTDYKSWPLDLLADYIEKKHHRYVVEKTAEILPYLD
                     KVCRVHGERHPELLEINEHFNKTAGELAMHMKKEELILFPFVRKLVNAKHEGSKLITP
                     PFGTVQNPIEAMMQEHTNEGDRFRKIEELSNNYTPPQDACNTYGVTLGLLKEFEQDLH
                     LHIHLENNILFPKAIELEKELS"
     gene            complement(4971..5405)
                     /locus_tag="PLQ70_06615"
     CDS             complement(4971..5405)
                     /locus_tag="PLQ70_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013687923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQE34325.1"
                     /translation="MFSKACEYGIRASIFIAEQSLLDQKVSLKDIAKAIDSPEAYTSK
                     ILQQLSKNAIITSEKGPTGGFSMNKQELEVVKLSTIVTAIDGDSIYKGCGLGLKNCNE
                     MMPCPAHNQFKIVREELRKMLETTLVMSLTTDFKDGLTFLKR"
     gene            complement(5487..5735)
                     /locus_tag="PLQ70_06620"
     CDS             complement(5487..5735)
                     /locus_tag="PLQ70_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34326.1"
                     /translation="MSTFRTSQNQANPNKLNNILSTLIFILILNVTIQIWLLYASLNN
                     ALENNREILIPAFIASLILFLIGFGWLYYLPSGNKQQK"
     gene            complement(5754..6269)
                     /locus_tag="PLQ70_06625"
     CDS             complement(5754..6269)
                     /locus_tag="PLQ70_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HQE34327.1"
                     /translation="MSKEDNLNKNWKKDFPILKQQATNVSRRDFAKFLTLVSGGLMVG
                     SGLVAAKAYLIPKEEVEGEHFVCKKDEVPVGGTRAFVIEGSTIPYILIHLESGDFKAY
                     EQKCTHLSCSVFYKPGTGIIHCPCHEGSFDAKTGDVLGGPPPRALPSLEVFFKENDIY
                     VKASHKEERAV"
     gene            complement(6373..6942)
                     /locus_tag="PLQ70_06630"
     CDS             complement(6373..6942)
                     /locus_tag="PLQ70_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQE34328.1"
                     /translation="MNYTSFNKNEEFFVDMQRCIGCKACEMACAECETNGQESLIHVN
                     YVDRASTVQTTVQVCMHCDDPVCANVCPADAISKDENGIVHTANTERCIGCSNCVMAC
                     PFGVPKKEEAYDLMMKCTMCYDRTSIGKKPMCATVCPSGALFFGTREEIQEMRPNSTP
                     INTFIFGKEIVNTKVNIMMPKGSTELVIY"
     gene            complement(6959..>8265)
                     /locus_tag="PLQ70_06635"
     CDS             complement(6959..>8265)
                     /locus_tag="PLQ70_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HQE34329.1"
                     /translation="KIREAAELWGKAKTSFLLHARGIEHHSKGVDNVVGCINLVLATG
                     RIGRPYCGYGTITGQGNGQGGREHGHKCDQLPGNRDIENPEHRKYISEVWGINEKDLP
                     GKGLSAYEIIEAIHRGEIKGLISICFNPLVSLPNSNYVREALEKLEYYVCIDFFLNET
                     ARHADIVLAGSLQEEEEGTTTSAEGRVIRIRQAVTPPGDARTDTSIILELAKRLGAED
                     KFTFENSEAIFNELRLASKGGTADYYGITYKRIEDEMGVFWPCPEIGHPGTPRLWEDK
                     KFKTPDGKAHFNAAPYKLPGEVTDADFPVILTTGRVVSQYLSGTQTRRIGKLVDQYPE
                     PLLEIHPKLAQQFGIKQRELIKVTTRRGVGIFPANIVETIREDTVFIPYHWSGKKSAN
                     QLTPGTLDPISKIPEFKVCACHLEPLKEIAPPSKESTAYASV"
BASE COUNT         2457 a         1572 c         1376 g         2860 t
ORIGIN      
        1 cgattttatt ggtattgaag gcgaattgtt tacgactaaa gtgggtgcac aatgtatccg
       61 tgtagataaa tttacttttt tgagtaaaac cttgcgtccg ttgccattgc caaaagtgga
      121 tgaagacgga aaagttcacg atgcctttaa cgatgccgaa ttgcgctaca gaatgcgtta
      181 tgtggatttg acggtgaacc aaaacgtgaa agacactttc atcaaacgca ccaagttgtt
      241 tactgcaatg cgcggttatt ttaacgatgc tggctatctt gaagtggaca ctccagtttt
      301 gcaatccatt cctggtggtg cttcggcaag accttttatc acgcaccaca actcgcttga
      361 cattccgctt tatatgcgta ttgccaacga attgtactta aaaagattga ttgttggtgg
      421 atttgaaggt gtttacgagt tttctagaaa cttccgcaac gaaggaatgg acagaacgca
      481 caacccggaa tttaccgcaa tggaaatata tgtagcctac aaagactaca actggatgat
      541 ggaatttgcc gaaggtttgc tggagcattg cgcaattgcc gtgaacggaa ccagcgaagt
      601 gacttttggc gaacaccaaa tcaatttcaa ggcgccttat gcccgcgtga caatgacaga
      661 ttccatcaaa cattttactg gatttgatat ttctggtaaa acggaagaag aattgtttgc
      721 cgctgccaaa ggaatgggaa ttgaggtgga cgaaacgatg ggtaaaggaa aattgattga
      781 cgaaattttc ggtgccaaat gtgaaggaaa ttatattcaa cccactttta ttaccgatta
      841 tccaaaagaa atgtcgccac tttgcaaaga acaccgcgac aatccagatt tgaccgagcg
      901 ttttgaattg atggtttgtg gtaaagaaat tgccaacgct tactcggaat tgaacgaccc
      961 aattgaccaa agagaacgtt ttgaagacca aatgcgtttg gctgcaaaag gtgatgatga
     1021 agccaacggc attatagacg aagatttctt gagagctctt gaatatggga tgcctccaac
     1081 atctggaatg ggaattggaa tggatcgttt gattatgtat ttgaccaaca atgcgtcgat
     1141 tcaggaggtg ttattgttcc cgcaaatgcg tccagagaaa aaacagacta tcgaattatc
     1201 tgaagaagag aaattaattg ttgctttact taaagcaaac gaaaataaaa tggatttagc
     1261 gcaactaaaa gttactgctg ccttaagcgg caagaaatgg gatgccgcca ccaaaggttt
     1321 gtcaaaaaac aacttgatta aagtgagtgt tgatggcgaa agcaaggtta tggaattggt
     1381 ggaataaaaa tatttaaaac attcatttaa tcagtttgat actaacattg atgactggtt
     1441 atgattgtaa tttgtacgta aaattgagtt tgggcatttt atatgattgt ataacctgtt
     1501 attttaataa ttcaatactt atggaaaaaa taaaaaaagg cgtgtttaaa actttgactg
     1561 cgtgagcaca attttattac aaatcacatc ataatgaata gttcaaagtt tagggttcga
     1621 atccctactc acgcgcgcaa aaatggagtt tcaattcgaa actccttttt tatgtccttt
     1681 gtttttctaa aaacagtacc aactcatcaa catttgtata tggattcaga ttgaaaacgg
     1741 caacatcgta attattttca agaataaata atctaacatt ttcagcattt gattttgcga
     1801 aagccaactt atcttttaat gttgaataat gtttaaaatc attcttttca taatttttca
     1861 ttccttcaca tttcttcaac tccttttcta attcagcctc ggaagcaaaa gcttttgtag
     1921 agtttttaaa atgtaacaaa taccaagatt caaaacaaat acttgaaaaa atcactttaa
     1981 tttttttatt agatgccagt ttgaaagctt cgtcacgttt gggttgattg tcgtgatcga
     2041 aaactacaaa tatttcttga aatccttctc gtttggcttt tttaattgct tcttcaacta
     2101 cacctttaca atctacttta ttgctttgaa aaatagctac accaatagtt ttaggcaaac
     2161 gaaaatcctc tttcattgca gtaaaataat tcttctctgt catgccttca caaagaatca
     2221 aaactttaga tctaaatgct cttgttttaa aagtattatc gggtcttcta gccataatta
     2281 tgaatttata aaatcaatca attcatatct aaaagtttcc ctggcaatat taggaacagc
     2341 tcccaatcta ccctctaaat accatttttc ataattccct tctcgaattc cttttaaatc
     2401 cgaaactgca aacatttctg tttcgccttt tacatttttc tctaccaaat tgatttggtc
     2461 tcttctcaat ttattttctg gacttaacaa agtggtgtcg tgagttgcaa aaataaattg
     2521 tgctcctttt gtattcactt ctggatcatt aaataatcct aaaatgtatt gcgcaatttc
     2581 aggatgcaaa cttctttcaa attcatcaac aataatcgtc gatcctgaac tcaatgctat
     2641 aaagaccaaa ggaacaaaag acataattct ttgtgttcca gaagattcat cttcgaaatc
     2701 aaattcaatt tgttcgaaat tatcattaaa gtgaaaagtt tttattacac cttctttctc
     2761 ttcaaaatta acaatttgag tatctaactt ttgtattaag gactttgaca atctaagaat
     2821 tacacctcca tcatttaggt cagtaaaaaa gagcttactt gctgtcttat tgaactcttc
     2881 tgaatgaaat aaatcagttg ttaatacaag cattgacgta atgctttcat aaacactttt
     2941 tgctatttca acattttcaa ttgcagcttt ccctaaaaat aattgatttt ccgttgtacg
     3001 ttgcttaatt gtttcgaaaa tcccttcata accatttcct ttactaaatt cttgcttctt
     3061 tcgttcaaaa acttttactt ttaaaccctt aggaaagtaa aatagtgatt ccgctataat
     3121 ttctttttga aaaacttcta ttgaataaat atgtaaaaca ttgtttatca aaaaatcaat
     3181 tgaaaaacaa gatggcttta gaacatttcc tttacataaa ataaagggat tatataactt
     3241 tatggtatca tttggtttat tatccgtcga attttttaca aaatttctaa aagcacccaa
     3301 tgctttaaca atattactct ttccacttgc atttggccca taaatggccg cagaagtcaa
     3361 tccgtaatag tctttgtttt ttgtagcatt ggggtttgtc tttataattc tgttaactgg
     3421 atttttaaaa tcactaggaa tcattgacaa ttcaaccatt tcgttaattg atctaaaatt
     3481 tgttattcta aagtttaaca gcatcacgta aagggtttat ttgtaaatat attacaaata
     3541 taagcattaa ttgtatttac accatgcaat tataaataaa aaagccagtt tcgaaatttt
     3601 gaaactggct tttttaggtt tacttttttt aaacaacaaa tacaaaatct attattccca
     3661 aaatggatct gtcaagttat ctacgaattt ttcgtctgaa tggttgattt ctatagattg
     3721 caactgttct gctgtggtaa ttttttgaat ttcaccaaac aaaatgcgtt cttcaaaacg
     3781 aatatgactt tctaattctt cttcaatcaa actaatgttt tttgaaattt cagtgttgga
     3841 ttcaaagagt tgttttatcc tatcgtgttc tgaaacggct tttttaatta attcattttc
     3901 gtttccaaga attggaaaaa catatttttc ttccacttca aaatgaggaa ttaaataatt
     3961 ttggtaaaac caatccgcat acttttttat gcgagatata tccacgcttt tttcaattcc
     4021 agctcttatt ttccaacaaa acagcaagcc gtgatgatgt tctctgctta atggctttaa
     4081 agattcactg cgcttgattg gcttatctat ttcaaaattg ttattctttt tcataattat
     4141 ttcaataaaa gcttctcaag attattgctt aatttatttt taatttaaga caattctttc
     4201 tccaattcga tggctttagg gaaaagtata ttattttcta aatgaatgtg caagtgcaaa
     4261 tcttgttcaa attcttttag cagaccaaga gttactccat aagtattaca agcatcttgt
     4321 ggtggtgtat aattattgct caactcttca atttttctaa atcgatcgcc ttcgttggtg
     4381 tgttcttgca tcatcgcttc gattggattt tgcaccgtac caaaaggagg cgtaatcaat
     4441 tttgaacctt cgtgttttgc gttgaccaat tttctaacaa atggaaataa aattaattct
     4501 tcttttttca tatgcattgc caattcacct gctgttttgt tgaaatgttc attgatttcc
     4561 aataattcgg gatgacgctc tccgtgcact ctacaaactt tatctaaata cggtaaaatc
     4621 tctgccgttt tttctactac ataacgatgg tgtttttttt cgatataatc tgccaataaa
     4681 tccaaaggcc aagatttgta gtctgttgta ttttctcctt gtgcttgaat tatagcatcc
     4741 aaatcattta ctactgattt tgaatcaatg tttttctttt cacaagcttc gttaatggtt
     4801 ctgttgcctt gacaacaaaa gtctattccg tattttttaa aaacggatgc tgtacggtaa
     4861 tctttagcta ccaattcacc gataatttga ttttcttgaa tgttcattgt atttatattt
     4921 aagtttatta ttactggact aaattgtccg ttattaaatt aatttttttt ttatcgtttc
     4981 aaaaatgtta atccgtcttt gaaatccgtc gttaaggaca taaccaatgt tgtttcgagc
     5041 atttttctta attcttcacg aacaatttta aattggttgt gcgcagggca aggcatcatc
     5101 tcattacaat ttttcaagcc taaaccacaa cctttataaa tactatcacc atcaattgca
     5161 gttacgattg tactcagttt tactacctca agttcttgtt tgttcatcga aaaaccgcct
     5221 gttggtcctt tttccgaagt gattatagcg tttttagaaa gttgttgcaa tatttttgac
     5281 gtataagctt cgggcgaatc gatggctttt gctatatctt ttaaactaac tttttgatcc
     5341 aagagcgatt gctcggcaat aaatattgat gctcttattc cgtattcaca agcttttgaa
     5401 aacattatta ttatattaga ttacaaagat agacaattaa ttttaatagt ggacatatac
     5461 atccactatt aataaagttt ttaaaattat ttttgttgct tattcccaga aggcaaataa
     5521 tacagccaac caaaccctat taaaaacaaa attaaagagg cgataaaggc gggtatcaaa
     5581 atttcacgat tgttttctaa agcgttgttc aacgaggcgt acaacaacca aatttggatg
     5641 gtgacattca aaatcaatat gaatatcaag gtcgaaagga tgttgttcag cttgttggga
     5701 ttcgcttggt tttgactggt tctaaaggta ctcatagttt tttgttttag tttttaaact
     5761 gctcgttctt ctttatggga agctttgaca tagatatcgt tttccttgaa aaacacttcc
     5821 agactcggca atgcccttgg tggtggccca cccaaaacat ctcccgtttt ggcatcgaac
     5881 gatccttcgt ggcacggaca atgtataatt ccggtgccgg gtttgtaaaa tacagaacac
     5941 gacagatggg tgcatttttg ctcataagcc ttgaaatctc cgctttcgag gtgaatcaaa
     6001 atataaggaa tcgtgcttcc ctcgattaca aaagcgcgag tgccaccaac aggaacctca
     6061 tctttcttgc acacaaaatg ttctccttct acttcttctt tgggaatcaa ataggctttg
     6121 gcagccacaa gtccacttcc taccatcaat ccgcccgaaa caagagtcag gaatttggca
     6181 aaatcacgac gactcacatt ggtcgcctgt tgttttagaa ttggaaagtc tttcttccaa
     6241 tttttattta aattatcttc ttttgacatt tctttgtgtt gtgaaaagtt tatttttagg
     6301 tttttcagaa gcaatcagct atctaaaatc ttccttctat tttctttatt ctatactcta
     6361 ttttctaaaa tcctaataaa taaccaactc cgtacttcct tttggcatca taatattgac
     6421 tttggtgttt actatttctt tcccaaatat aaaagtatta attggcgtac tgttcggacg
     6481 catttcttgg atttcttctc tcgtgccaaa aaacaaagca ccacttggac aaactgttgc
     6541 acacattggt tttttaccaa tactggttcg gtcgtaacac atcgtgcact tcatcatcaa
     6601 atcgtaggct tcttcttttt taggcacgcc aaacggacaa gccattacac aattcgaaca
     6661 accaatgcat ctttcggtat tagcagtatg tacgattcca ttttcgtctt tggatattgc
     6721 atcggcggga catacattgg cacaaactgg atcatcacag tgcatacaca cttgaacggt
     6781 ggtttgcaca gtcgaagctc gatccacata attcacgtga atcaaggatt cttgaccatt
     6841 cgtctcgcat tcggcgcaag ccatttcaca ggctttacag ccaatgcaac gttgcatatc
     6901 tacaaaaaat tcctcgtttt tattgaaact ggtgtaattc atttttactt tttatagatt
     6961 aaacacttgc atacgctgtt gattctttcg atggaggcgc aatttccttg agcggttcca
     7021 aatgacaagc acaaactttg aactccggga tttttgaaat tgggtctaat gttcctggtg
     7081 ttaactgatt ggctgatttt tttcctgacc agtggtacgg aataaaaacg gtatcctccc
     7141 gaatggtttc cacaatattt gctggaaaaa tcccaactcc tcttcgggtg gtgactttta
     7201 tcaattctct ttgtttgatt ccaaactgtt gtgccaattt aggatgaatt tccaataatg
     7261 gttctggata ttgatcgact aattttccaa ttcttcgggt ttgtgttccg ctcaaatatt
     7321 gagacaccac acgacctgtt gtcaaaatca cgggaaaatc agcatcggtt acttcgcccg
     7381 gaagcttgta cggagcggca ttaaaatgcg ctttgccatc aggtgtttta aattttttat
     7441 cttcccataa tcttggtgtt ccgggatgac cgatttctgg acaaggccaa aagacaccca
     7501 tttcgtcttc aattcttttg taagtaatgc cgtaataatc ggctgttcct cctttagaag
     7561 ccaaacgcaa ttcgttgaaa atggcttcgc tgttttcgaa agtaaattta tcctcagcac
     7621 ccaaacgttt cgctaattcg agaattatcg aagtatctgt tctggcatcg ccaggaggtg
     7681 tcacagcctg acgaattcgg attactctac cttcagcaga tgtagtggtt ccttcctcct
     7741 cttcctgcaa cgaaccagcc aaaactatat cggcgtggcg tgcagtttcg ttcaagaaaa
     7801 agtctataca cacataatat tccagttttt ccaaagcttc gcgaacgtaa ttgctgttcg
     7861 gtaaagacac caatggattg aaacaaattg aaatcagtcc cttaatttcc ccacgatgaa
     7921 tggcttcgat gatttcatag gccgaaagtc cttttccagg taaatctttc tcgttgattc
     7981 cccaaacttc cgaaatatac ttgcggtgtt ctggattttc gatatctcta tttccaggta
     8041 attgatcgca tttatgaccg tgctctctac cgccttgacc gtttccttga ccagtaatag
     8101 ttccataacc gcaatacggt cttccgattc ttcctgttgc gagtaccaaa ttgatgcaac
     8161 ccacaacatt atccacgcct ttcgagtggt gttcaattcc acgggcgtgc agcaagaaac
     8221 tcgttttggc tttgccccat aattcggcgg cttcgcgtat tttat
//
LOCUS       DAOSNN010000104         8238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8652_length_8238_cov_5.616033, whole genome shotgun sequence.
ACCESSION   DAOSNN010000104 DAOSNN010000000
VERSION     DAOSNN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8652_length_8238_cov_5.616033"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..867
                     /locus_tag="PLQ70_06640"
     CDS             40..867
                     /locus_tag="PLQ70_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQE34330.1"
                     /translation="MKTKIAIQGIKGSFHHQVAQEYYSHDVEVDECMSFEELVDSVLS
                     GKTDQAVMAIENSIAGPIIPNYALIDKNNLHIIGEHYLSIHQNFMALKGQKIEDIQEV
                     HSHPIALLQCMQFLKKYPHIKIVEDKDTAETARRIHEKQLTGVAAIASKAASEMYDLE
                     ILAPEIQTINNNMTRFVIINKEEKFVEEDEINRASIKFELDHKRGSLAAVLNVMSDCK
                     LNLTKIQSLPKIETPWKYSFFVDVTFDKYEDYAKAKSLLGIMAEYFKVLGEYKNTKP"
     gene            932..>1944
                     /locus_tag="PLQ70_06645"
     CDS             932..>1944
                     /locus_tag="PLQ70_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQE34331.1"
                     /translation="MIATAKRLDIVEEYYFSSKLREVRQLASEGKPIINMGIGSPDLK
                     PSQAVIDAVTLAMQEENAHQYQSYQGLPELRNGMANFYQNNYGVALNPNTEILPLMGS
                     KEGIMHISLAFLNEGDQVLIPNPGYPTYSSVTNLVGAVPVYYDLKEANNWEPDFEALE
                     KLDLSKVKLMWLGYPHMPTGARGNLKLFEKLVAFAKKHNILLINDNPYSFVLNDNPMS
                     LLQVEGAKDVALELNSLSKTFNMAGWRVGMVLGNAACIDAVLKVKSNMDSGMFFGIQK
                     GAIEALKSDQSWFDSMNAIYKKRRILTEQLAEKLGCEVYKEGVGLFVWAKLPAGITSA
                     EDF"
     assembly_gap    1945..2044
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2045..2886
                     /locus_tag="PLQ70_06650"
     CDS             <2045..2886
                     /locus_tag="PLQ70_06650"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HQE34332.1"
                     /translation="VIGIGLIGGSMVLDIKSLHPDATIYGIDNNESHLAEAIALGVVD
                     QAATFDDLAEADYVIVSVPVDVALTVLPKVLDAVGENTIVFEVGSTKTPICEAIANHP
                     KRRNFIATHPIAGTEFSGPSAAIKNLFQGKTNIICEVEKTTFKLQEKALDLFKSMGMR
                     IRYMDPKSHDKHIAYVSHLSHISSFMLGKTVIDKEKVEQDIFDMAGSGFESTVRLAKS
                     SPAMWTPIFKQNKKQVVKGLEEYISNLIHFKELLENDDYDAIFNEMQSVNKIKEILNG
                     MKK"
     gene            2959..4038
                     /locus_tag="PLQ70_06655"
     CDS             2959..4038
                     /locus_tag="PLQ70_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HQE34333.1"
                     /translation="MENKKEMRNWLNDFNLSHPLVIAGPCSAETEEQVLKIAHELKDS
                     DVSVFRAGIWKPRTRPGGFEGVGEIGLKWLKKAKAETGMLMGTEVATAAHCKLALEYD
                     IDVLWVGARTTANPFAVQEIADTLKGTDKIVLIKNPVNPDMALWLGGVERLYAAGIEK
                     LGVIHRGFSTYEKTKYRNIPEWQIAIELQNKFPDLPLIIDPSHITGNRDMILEVTQEA
                     LDLNYDGMIIETHIDPENAWSDAAQQVTPDALKQILKDLKIRNLSGDSDFESKMTKLR
                     ANIDVLDANLLELLGKRMKVADEIGQVKKENNVAVLQNNRWNEIQAKMVAEGAKKGLT
                     EEFIVKLFRGIHQESIEHQERILNS"
     gene            4202..5179
                     /gene="rsgA"
                     /locus_tag="PLQ70_06660"
     CDS             4202..5179
                     /gene="rsgA"
                     /locus_tag="PLQ70_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQE34334.1"
                     /translation="MTGTVYKSTGSWYTVKSERGDFIECRMKGKFRIKGIKSTNPIAV
                     GDIVDYELEETSDNVTGTIHKIHDRKNYIVRKSVNLSHQMHIIASNIDCVFLLITINN
                     PPTTTSFIDRFLVTAEAYGIETILVFNKIDTLDDATLDEQLYMQHIYQEIGYQCLRVS
                     AATGKGVEELKQLMIEKVTMFSGHSGVGKSTLVNALEPTLNLKTKNISEQSKQGQHTT
                     TFAEMYDLSFNARIIDTPGIKGFGIVDMEKEEISGYFPEFFKLKEQCKFNNCLHKEEP
                     HCAIKAALEKDEIAWSRYNSYLKILEGDDENYRTDSFDEDRKASDETRK"
     gene            5210..5569
                     /locus_tag="PLQ70_06665"
     CDS             5210..5569
                     /locus_tag="PLQ70_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009420544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQE34335.1"
                     /translation="MSENIIKTKSFNFALRIVKLYQFLNQEKREYVLSKQLLRSGTSV
                     GALVREAEQAESRLDFIHKLAISQKEANESDYWLELLFQSDYLNEIQYQSLKSDIVEI
                     NKILASIIISTKQNLKK"
     gene            5622..6074
                     /gene="dtd"
                     /locus_tag="PLQ70_06670"
     CDS             5622..6074
                     /gene="dtd"
                     /locus_tag="PLQ70_06670"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQE34336.1"
                     /translation="MKTVIQRVSQASVIIDAKIVADIKSGLLVLVGIEDADTQEDTNW
                     LCQKIANLRIFGDENDVMNLSVKDIDGEVIVVSQFTLQASTKKGNRPSYIKAAKPEVA
                     IPIYENFVHQLETELGKRIQTGIFGADMKVLLQNDGPVTILIDSKNKM"
     gene            6126..8033
                     /locus_tag="PLQ70_06675"
     CDS             6126..8033
                     /locus_tag="PLQ70_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HQE34337.1"
                     /translation="MKFLLSSLIAFLFATTISAQKTEYTSSSIPDSLKQNANAVVRLD
                     QIDILISSQRSMNIKQKRVVTVFNEKGQEAINAYEYYDKKTIVENIQATVFDAFGNEI
                     KKIKRKDFRDQCATDGGTIFSDSRFVYLNYTPTQYPFTIIYESEIQTSTTAFIPSWYP
                     LSDYHLSVEKSVLNVSYPNNLGFKKMEFNFSKFKINKATDTSTQLSYVAVNIPALKQE
                     NYSPNYSVFPKVMMGLELFHLEGVDGNAKTWKEFGQWYSDKILTGTTELSEETKSKIK
                     NLVGNEKDPIKKAAIVYKYLQEKSRYVSIQVGIGGWKPMLANDVDRLGYGDCKALSNY
                     TKALLNAVDVPSYNTLLYGDRNKHDIQSDFVSMQGNHMILSIPNGDNYVFLECTSQDI
                     PFGYQANFTDDRNVLIIKPEGAEIVKTKNYENKDNSQISKGNYSIAENGDFSGKISIV
                     SEGSQYSPKYRLENTQPTEREKHYKEYWGNINNLKIDKITFLNDKEKVSFTENAEISA
                     VNYGAISSNKMIFAIDAFNQNSEVVKRIRNRKNPFEIQRGYYDNDEIAIALPTGFSIE
                     SIPNNFELNTKFGEYKTEIIKVDNTNLIYKRSIFIKKGLYKNTEYDDYRLFMEQISRN
                     DNAKIILTKNL"
     gene            8038..>8238
                     /locus_tag="PLQ70_06680"
     CDS             8038..>8238
                     /locus_tag="PLQ70_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34338.1"
                     /translation="MKTYVQLFILFFVFSFANAQEFKLGKVSIAELEEKQHPKDPSAV
                     AAILFKKGEVKFEYTQSDGFTMF"
BASE COUNT         2967 a         1233 c         1583 g         2355 t
ORIGIN      
        1 acaatcctga tgaaaatcag gatttttttt tgagtataaa tgaaaacaaa aattgcaatt
       61 caaggtatta aagggtcatt ccatcatcag gtggcgcagg aatactattc tcatgatgtt
      121 gaggtagatg aatgtatgtc gtttgaagaa ttggtagata gcgttttgtc tggaaaaacg
      181 gatcaagcgg tgatggcaat cgaaaattct attgcagggc caattattcc taattatgct
      241 ttgattgaca aaaataattt gcacattatt ggcgagcatt atttaagtat tcatcaaaat
      301 tttatggctt tgaaaggtca aaaaattgag gatattcaag aagtacattc gcatcccatt
      361 gctttgttgc agtgtatgca gtttttgaaa aaatacccgc acataaaaat tgtcgaggat
      421 aaagatacgg ccgaaacagc tagaagaatt cacgaaaaac agttgacagg ggttgcggca
      481 attgctagta aagcagcttc tgaaatgtat gatttggaga ttttggcacc agaaatccaa
      541 acgattaaca acaatatgac tcgttttgtg attatcaaca aagaagaaaa atttgttgaa
      601 gaagacgaaa ttaatagagc ttccatcaaa ttcgaattgg atcacaaacg tggaagttta
      661 gccgcagttt tgaacgtaat gagcgattgc aaattgaatt tgacgaaaat tcaatccttg
      721 ccaaaaatag aaacaccttg gaaatattcg ttttttgtgg acgtgacttt cgataaatac
      781 gaagattacg ccaaagcaaa atcgttattg ggaattatgg cggaatattt caaagtgttg
      841 ggcgaatata aaaatacaaa accgtaaaaa ggaaattcca aaagacaaaa aacaaattcc
      901 aaaaggaaca gcgttaggaa aaattacaaa tatgattgca acagcaaagc gtttagacat
      961 agttgaagaa tattattttt cttcaaaact tcgcgaagta agacaattgg cttccgaagg
     1021 gaaaccaatt atcaatatgg gaattggtag ccccgatttg aagccctcac aagccgttat
     1081 tgatgcagta actttggcaa tgcaggaaga aaatgcacat caataccaga gttaccaagg
     1141 cttgccggaa ttgagaaacg gaatggccaa tttttatcaa aacaattatg gcgttgcctt
     1201 gaatccgaac actgaaattt tgcctttgat gggttccaaa gaagggatta tgcatatttc
     1261 attggcattt ttgaatgaag gagatcaagt tttgattcct aatccaggtt atcctactta
     1321 ttcttcggta acgaatttgg ttggagccgt gcctgtttat tacgatttga aagaagccaa
     1381 caattgggaa cctgattttg aagctttgga aaaattggat ttatcgaaag taaaactaat
     1441 gtggttgggt tatccgcata tgccaacggg agcgcgagga aacttgaagt tgttcgaaaa
     1501 gttggtggct ttcgccaaaa aacacaacat attgttgata aacgacaatc catatagttt
     1561 tgtcttgaat gacaatccga tgagtttgtt gcaagtggaa ggtgcaaaag acgtggcatt
     1621 agaattgaat tctttgtcaa aaaccttcaa tatggcaggc tggcgtgtgg gaatggtttt
     1681 aggaaatgca gcttgcatcg atgcagtttt gaaagtaaaa agcaatatgg acagcggaat
     1741 gttcttcgga attcaaaaag gagcaattga agccttgaaa agcgatcaat cttggttcga
     1801 ttcgatgaat gcgatttaca agaaaagaag aatcttaaca gaacaattgg ccgaaaagtt
     1861 gggttgtgaa gtttacaaag aaggcgttgg attgtttgtt tgggcaaaat tgccagcagg
     1921 aatcacgtcg gcagaagatt ttatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnacgtaa taggaatagg attaattggt ggttcgatgg ttttggacat caaatcacta
     2101 catccagacg cgactattta tggaattgac aacaacgaaa gtcatttagc ggaagccatt
     2161 gctcttggag ttgttgacca agcggcgaca tttgatgatt tggcggaagc cgattacgtg
     2221 attgtttcgg ttccagtgga tgttgcattg actgttttgc caaaagtttt ggatgctgtt
     2281 ggggaaaata caattgtttt tgaagtgggt tccaccaaaa cacccatttg cgaagccatt
     2341 gcaaatcatc ccaaaagaag aaatttcatt gccacgcatc ctattgcggg aacggaattt
     2401 tcagggcctt cggcggcgat aaaaaacttg tttcaaggga aaacgaatat catttgtgag
     2461 gtcgaaaaaa cgactttcaa attacaggaa aaagccttgg atttgttcaa aagtatggga
     2521 atgagaattc gatatatgga tccaaagtca catgataagc acattgctta tgtatcgcat
     2581 ttgtcgcaca ttagttcgtt tatgcttgga aaaacggtga ttgataaaga gaaagtcgag
     2641 caagatattt ttgatatggc gggttctgga ttcgaaagta cagtgcgttt ggctaaaagt
     2701 tccccagcta tgtggacacc gattttcaaa cagaataaaa agcaagtggt caagggttta
     2761 gaagaatata tttcgaatct tatccatttc aaagaattat tagagaatga tgattacgat
     2821 gcgattttca acgaaatgca gagtgtcaat aaaataaaag aaatcctgaa cggaatgaaa
     2881 aaatagacaa ttgcaaaaat caattgcaat gtcaatagaa caacacacaa taaaacaata
     2941 aaaattaaat tagaaaagat ggaaaacaag aaagaaatga gaaattggtt gaacgacttc
     3001 aatttgtctc atccattagt gatagcagga ccttgtagtg ctgaaacaga agagcaagta
     3061 ttgaaaattg cccacgaatt gaaagattct gatgtaagcg tttttagagc tggaatctgg
     3121 aaaccaagaa ctcgtccagg aggatttgaa ggtgttggag aaattggatt gaaatggttg
     3181 aaaaaagcaa aagcggaaac aggaatgcta atgggaactg aggttgccac tgctgcacac
     3241 tgtaaattag ctttggaata tgatatcgat gttttatggg ttggtgctcg tacaacggca
     3301 aatccatttg cggttcaaga aattgcggat accttaaaag gaacagataa aatcgtttta
     3361 atcaaaaatc cagtaaatcc tgatatggct ttatggttag gtggagttga gcgtttgtat
     3421 gcagccggaa tcgaaaaatt aggagtaatt cacagaggtt tttctactta cgaaaaaacc
     3481 aaatacagaa acattccaga atggcaaatt gctatcgaat tgcaaaataa attccctgat
     3541 ttgcctttaa ttatcgatcc ttctcacatt acaggaaacc gtgatatgat tcttgaagta
     3601 actcaagaag ctttggattt gaattatgat ggtatgatta tcgaaacgca tattgatcca
     3661 gaaaatgctt ggtctgatgc agctcaacaa gtaactccag acgctttgaa acaaattttg
     3721 aaagacttga aaattagaaa cttgagtgga gattcagatt ttgaaagtaa aatgacaaaa
     3781 ttaagagcta atatcgatgt tttggatgct aatttgttag agcttttagg aaaacgtatg
     3841 aaagtagccg atgaaattgg tcaagtgaaa aaagaaaaca atgtagctgt tttacaaaac
     3901 aaccgttgga atgaaattca agccaaaatg gttgccgaag gtgctaaaaa aggtttgaca
     3961 gaagaattta tcgttaaatt gttcagagga attcaccaag aaagtatcga acaccaagag
     4021 agaatattga attcttaatt caataatttt tataaaatta atctccacag ttactataat
     4081 tgtggggatt ttttgttttt aggtattgca aaaatatcag acattaaact ttaaacgaaa
     4141 aacaatcatt tatctttgca aacaaatcta aaatctgaat tcgtaattct taaatcaaaa
     4201 aatgacagga accgtttata aatctacagg aagttggtac acagtgaaat ctgaacgagg
     4261 agattttatc gaatgccgta tgaaagggaa gttccgaatc aaaggtatca aaagcacgaa
     4321 cccaattgct gtaggtgata ttgtcgatta tgagcttgaa gaaacatcgg ataatgttac
     4381 gggaactatc cataagattc acgacagaaa gaattatatt gttcgaaaat ctgtaaatct
     4441 ttcacatcaa atgcatatta ttgcatccaa tattgattgt gtttttcttt tgataactat
     4501 caataacccg cctacaacta ccagttttat agaccgtttt ttggtcacgg ccgaagctta
     4561 tggtatagaa acgattttgg tttttaataa aatagatacg ctggacgatg ctacactaga
     4621 cgagcaattg tatatgcagc acatttatca ggaaatagga taccaatgcc tgcgagtttc
     4681 tgctgccaca ggaaaaggag tagaagagtt aaaacaattg atgatagaaa aagtaacaat
     4741 gttttcgggt cattctggtg tgggaaaatc gacattggtc aacgctttgg aaccaacttt
     4801 gaatctgaaa acaaaaaata tttctgaaca aagtaaacaa ggacaacaca caacaacttt
     4861 tgccgaaatg tatgatttga gttttaatgc tcgaataatt gacactccag gaatcaaagg
     4921 tttcgggatt gtggatatgg aaaaggaaga aattagtggt tattttccag aattcttcaa
     4981 gttgaaagaa caatgcaaat tcaataattg tctgcacaaa gaagagccac attgcgccat
     5041 caaagcagct ttagaaaaag acgaaattgc ttggtcgcgc tacaatagtt atttgaaaat
     5101 cctcgaagga gacgatgaaa attatcgcac cgattcattt gatgaagatc gaaaagcaag
     5161 tgatgaaacc agaaaatagt tgtgaattgt gaatggtaaa ttgtgaatta tgtcagagaa
     5221 tataataaaa actaaatcat ttaatttcgc tttgaggatt gtgaaattgt atcaattctt
     5281 aaatcaggaa aaaagggaat atgttttaag taagcagctg cttcgaagcg gaacatctgt
     5341 tggagcatta gttagagaag cggaacaagc tgaaagtaga ttagatttca ttcacaaatt
     5401 ggcaatttcg caaaaagaag ccaatgagag tgattattgg ttagagttat tgtttcaatc
     5461 agattaccta aacgaaatac aatatcaatc tttaaaatca gatatagtag aaatcaataa
     5521 aattctcgca tccatcatta tttcgactaa acaaaatcta aaaaaataat tgtcaattat
     5581 ctcagtaatt cacaatttac cattcacaat tcaccattat tttgaaaacc gtaattcaaa
     5641 gagtttccca agcctctgta atcattgatg ctaaaattgt agccgatatt aaaagtggct
     5701 tattagtttt ggtcggaata gaagatgcag atacacaaga agacactaat tggctttgcc
     5761 aaaaaatcgc aaaccttcgc atttttggtg atgaaaacga tgtgatgaac ctttcggtaa
     5821 aagatattga tggcgaagtg attgtggttt ctcaatttac ccttcaagcc tcgaccaaaa
     5881 aaggcaatcg cccatcttat attaaagcgg caaaacctga agttgccatt ccaatctatg
     5941 aaaacttcgt tcatcaatta gaaactgaat tgggcaagag aattcaaacc ggaatttttg
     6001 gagccgatat gaaagtattg ctccaaaatg atggtcctgt aacaatttta attgactcta
     6061 aaaataaaat gtaatttagg attattctta agaaagattt cataaatttg ataaaacatt
     6121 tttttatgaa atttcttctt tcttcattga ttgccttttt gtttgccact acgatttctg
     6181 ctcaaaaaac cgaatacacc agctcatcaa ttccagacag cctaaaacaa aatgccaatg
     6241 ccgttgtgcg tttagaccaa attgatattc taatttcatc ccaacgaagt atgaatatca
     6301 aacagaaacg agtcgttaca gttttcaacg aaaaggggca ggaggctatt aatgcttatg
     6361 aatattatga caagaaaaca attgtagaaa atatacaagc aacagttttt gatgcctttg
     6421 gaaatgaaat aaaaaaaata aaacgaaaag actttagaga tcaatgtgcc accgatgggg
     6481 gaacgatttt ttcagatagt aggtttgttt atttaaatta cacacccaca caatatccat
     6541 ttaccatcat ttatgaaagc gaaattcaaa cttctacaac tgcttttata ccatcatggt
     6601 atcctttaag cgattatcac ttgagtgttg aaaaaagtgt tttgaatgta agttatccaa
     6661 acaatttggg ttttaagaaa atggaattca atttttctaa attcaaaatc aataaagcaa
     6721 cggatacatc aactcaactt tcttatgtag ctgtcaatat tcctgcatta aagcaagaaa
     6781 attatagtcc taattattct gttttcccaa aagttatgat gggtttagag ctttttcatc
     6841 tagaaggtgt tgatggaaat gcaaagactt ggaaggaatt tggacaatgg tattctgata
     6901 aaattttaac aggaaccact gaattatcag aggaaaccaa atcaaaaatc aaaaatctag
     6961 tcggcaacga aaaagaccca atcaaaaaag cagcaattgt atataaatat ttacaggaaa
     7021 aatcaagata tgtgagtatt caagttggaa taggagggtg gaaaccaatg cttgctaatg
     7081 atgtagatcg attaggttat ggtgattgta aagcgttgtc aaactacaca aaagctcttt
     7141 taaatgctgt tgatgttcct tcctataata cgcttttata tggcgataga aacaagcacg
     7201 atattcagtc agactttgtc tcaatgcaag gaaatcatat gatattatcg attccaaacg
     7261 gggataatta tgttttttta gaatgtacca gtcaagatat tccatttggg tatcaagcca
     7321 attttaccga tgacagaaat gttttaatca ttaaacccga aggtgctgag attgttaaaa
     7381 caaaaaatta tgaaaacaag gacaactctc aaattagtaa aggcaactat tcaatagctg
     7441 aaaatggtga tttttcagga aaaatttcga tagtttccga aggatcacaa tacagtccaa
     7501 aataccgttt agaaaacact cagcccaccg agagagaaaa gcattataaa gaatattggg
     7561 gcaatatcaa caatttgaaa attgataaaa tcactttttt aaatgacaag gaaaaagtga
     7621 gttttaccga aaacgctgaa attagtgcag taaattatgg agcaatttca agcaataaaa
     7681 tgatttttgc aatagatgct ttcaatcaaa actctgaagt tgttaagaga attcgaaacc
     7741 gtaaaaaccc attcgagatt caaagaggtt attatgacaa tgatgaaatt gctattgcat
     7801 taccaacagg gttttctata gaatctatcc ccaataattt tgaattgaat accaaatttg
     7861 gagaatacaa aaccgaaatt ataaaagtgg acaatactaa tttaatttat aaacgttcga
     7921 ttttcatcaa aaaaggattg tataaaaaca ctgaatacga tgattatcgt ctttttatgg
     7981 aacaaatttc tagaaacgat aatgccaaaa taatacttac aaaaaacctt taaaaaaatg
     8041 aaaacctacg ttcaattatt tattttattt tttgtctttt cctttgccaa tgcccaagaa
     8101 ttcaaattag gaaaagtttc tatagccgaa ttagaagaaa aacaacatcc caaagatcct
     8161 tcggctgtgg cagccatatt attcaaaaaa ggagaagtca aattcgaata tacgcaaagc
     8221 gatggattta caatgttt
//
LOCUS       DAOSNN010000105         8226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8664_length_8226_cov_5.101089, whole genome shotgun sequence.
ACCESSION   DAOSNN010000105 DAOSNN010000000
VERSION     DAOSNN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8664_length_8226_cov_5.101089"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PLQ70_06685"
     CDS             complement(<1..1002)
                     /locus_tag="PLQ70_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HQE34339.1"
                     /translation="MIRIKPFFVLNFIVLLSFWNAHSQYDAKYPKNEFRAVWIATVVN
                     IDWPKAGTDSVNKQKADFIEILETYKKLNYNAIIVQIRSTGDAFYPSKLAPWSRYITG
                     KEGQAPKPYYDPLAWMIEQAHQRGFEFHAWLNPYRATFDTKTETLSKEHDFFKHRDWM
                     IKYGETGKEKYYYNPALPEVQSHLTAVVEEVVKNYDVDAIHFDDYFYPYKVSGKEFND
                     TASFKKYSYKQSLEDWRRDNVNNFVKNIYYSIKRIKPWVQFGISPFGVWRNKSVDPKG
                     SDTQSGQTNYDDLYADPIAWMEGNYIDYMLPQLYWSIDHKTASYSKLIKWWADNSSNA
                     "
     gene            complement(1155..2426)
                     /locus_tag="PLQ70_06690"
     CDS             complement(1155..2426)
                     /locus_tag="PLQ70_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HQE34340.1"
                     /translation="MKKERLISLDVFRGFTIMVMTIVNNPGSWAHIYPPLEHAEWNGC
                     TPTDLVFPFFIFIMGTAIPFAMPDKHFDSAIFNKIVVRSIRIFCLGLFLNYFGKIHFL
                     GLEGIPLLTIRILITTIVGIALLGNFNQKIKTYLAFGVLGILLFLAYSSIESFQEVRI
                     PGVLQRIGIVYFFTSLLFLKTSPKIQIGITVLLLLIYWFLMTCIPVPGFGAANFEKGT
                     NLAAWLDNSILNGHLWSATKTWDPEGILSTIPAIATGIIGMLIGQLLNIQATKIEITK
                     KLIVLGISLIALGLIWSLVFPINKSLWSSSYVLYTAGLAISILMILYYLIEIANYKKW
                     TKLFLIWGVNPMIVFFLSGILPRVWGMIEIQNPEIASEKININEYFYNYVITPQFLNP
                     LNASLAYALLYVLFWTIILYIFYKQKLIFKV"
     gene            complement(2460..5456)
                     /locus_tag="PLQ70_06695"
     CDS             complement(2460..5456)
                     /locus_tag="PLQ70_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQE34341.1"
                     /translation="MKNYFIKITFYLTFAFLASNCIGLKKDQKTIDAKKETTISANKE
                     IYVAKNKTVGATFFKDSDEENHWVDSIYTKMSLEEKIGQLFMVAAYSNKDSLHAKKID
                     SLVQGYKVGGIIFFQGGPVRQAQLTNRFQSKANVPLFIAIDAEWGLAMRLDSTYRYPW
                     NMTLGAIQDLKLIEKVGSNMGNECKRMGIHFNFAPVLDINTNPKNPIIGNRSFGESKV
                     NVADKAIAFLKGEQSQGVFCTGKHFPGHGDTATDSHKTLPTINFSKERIDLVELYPFK
                     RMFDDGLASVMVAHLNIPSLETKQDCPSSLSYNVVTNLLQKELGFNGLIFTDALGMKG
                     ASNFMQPGDIDLAAFMAGNDILLCPENVPLAVEKIKIAYNQGTISEERLAHSVKKILH
                     YKFKAGLNKPNPIDLANLYQDINKTENDALQYQLFENAITVLNNKSDVLPIKKLDQKI
                     AYVKLGDDDNSSFLTTLKKYTEITEVTASSIDSLTLKLKNFDTVIIGFHKSDLAWKNH
                     DLTEKELLWLQEIAKNNKVILDVFAKPYSLLPITNFDEIEGLVVSYQNSTISQIVSAE
                     LIFGAIEAKGKLPVSINSNFAVNTGVSTQKLDRLGFTAPENEGMNPMILNQIDEYAKK
                     AIDGKMTPGAQVLVARNGKVIYQKSYGYQTYDKEKLVTNSDLYDLASITKMVATLPNV
                     MQLYDQNKVTLDTKLSKMLPSLSKSNKKDIGFKNLLSHYAGLQAWIPFYKATLDSAKM
                     PSKKYYQKTFDAQHIRQIADSIFIRNDYHDTIIKIIADSPLDKKIEYKYSDLTFILLK
                     EYLERVNHKKLDVLVQEKFYKSLGMNYTLFNPLKKFDKNSIAPTEKDNYFRHQTLQGY
                     VHDMGAAMEGGVSGHAGLFSNAMDVAKMMQLYLQKGSYGNQRYFSEKTFNDFNTCYFY
                     TEGVNRGLGFDKRIGKDGSTCGCVSPASFGHTGFTGNMAWADPETQIIYVFLSNRTYP
                     EYVENTLVKGKIREDIQQVIQNAIVK"
     gene            complement(5491..6996)
                     /locus_tag="PLQ70_06700"
     CDS             complement(5491..6996)
                     /locus_tag="PLQ70_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HQE34342.1"
                     /translation="MTPSAILILIIVYFGLLFYISHSVSKKNDGNDAFFKANKNSKWY
                     LVAFGMIGTALSGVTFISVPGEVGSPNGEQFKYFQFVLGNAIGFIIITKLLLPLYYRM
                     NLTSIYGYIEQRMGHYSYRTAASIFLVSRTISSAFRLYLVVIVLQRFVFDYYNIPFSL
                     TVLISLLLIFSYTYRGGLKTIIITDTLQTFFLVSSVFLTIYFICNSLDLSLVGAFHEV
                     KDSNYSQIFFFDDFMTNKFHFAKQILGGMFVTIAMVGLDQDLMQKNLSCKNIKDAQKN
                     MFTFTGIFVVINIFFLSVGALLYIYASKNGIEIPIDSITNKPRTDLLFPEIALNHLTV
                     IPAIVFLLGLIAATFATTDSALTALTTSFCVDFLGMEKSENQAKKNIIRTRHLVHLSF
                     SALLFLIILVFNSINDSSVVGMIFKVASYTYGPLLGLYAFGLFHKGRKVNDKLVPYIC
                     FLSPAITYLVSENSATLFFGYIFDNELIIINALITFIGLYSISKKAEKIDF"
     gene            complement(7019..7837)
                     /gene="murQ"
                     /locus_tag="PLQ70_06705"
     CDS             complement(7019..7837)
                     /gene="murQ"
                     /locus_tag="PLQ70_06705"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HQE34343.1"
                     /translation="MKKIYPDTEKESNYSNLDKMSTKELLENINAEDKKVAHAVETQI
                     PNIEKLVDAIVSKMKLGGRLFYIGAGTSGRLGILDASECPPTFGVSDQMVIGIIAGGD
                     FAIRKAVENAEDDFEQAWIDLKKHDINSLDVVIGIAASGNTPYVIGGLKKAQENSIIT
                     GSITCSNNSKISKEADYPIELLVGPEFLTGSTRMKAGTSQKMVLNMITTSVMIQLGRV
                     KGNKMVDMQLSNKKLVQRGVEMIVEALHIDEKLAAQLLKEHKSVRAVLLSRNKA"
     gene            complement(7916..>8226)
                     /locus_tag="PLQ70_06710"
     CDS             complement(7916..>8226)
                     /locus_tag="PLQ70_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HQE34344.1"
                     /translation="TFSFTPKPNFGAKEPLYNGQICYGQDLTTHKKVNRLELQWLIRS
                     YKMTTDKTKFFNAFFTKLAGTKQLQQQIENGESENKIRKSWKKGLKDFKTKRAKYLIY
                     "
BASE COUNT         2629 a         1404 c         1317 g         2876 t
ORIGIN      
        1 tgcattggat gaattatccg cccaccattt aatcaatttt gaataggaag ctgttttatg
       61 atcgatgctc caatacaatt gtggcaacat ataatctatg taatttcctt ccatccaagc
      121 aattggatcg gcgtataaat cgtcataatt cgtttgtcct gattgtgtat ctgacccttt
      181 tggatctaca gatttattgc gccaaacccc aaacggacta attccaaatt gcacccaagg
      241 tttgattctt tttattgaat aataaatgtt ttttacaaaa ttatttacgt tgtcacgtct
      301 ccaatcttca agcgattgtt tgtaactgta ttttttaaat gaagctgtat cgttaaattc
      361 tttgccacta actttatagg gataaaaata atcatcaaaa tggatggcat caacatcata
      421 atttttcaca acttcctcaa caacagcagt cagatggctt tgaacttcgg gtagagcagg
      481 gttgtagtag tatttttctt ttccagtctc tccatatttt atcatccaat cccgatgttt
      541 gaagaaatcg tgttctttgc tcaacgtttc tgttttggta tcgaaagttg ctctataggg
      601 atttagccaa gcatgaaatt cgaagcctct ttgatgtgct tgttcgatca tccaagctag
      661 aggatcataa taaggttttg gtgcttgtcc ttcttttccg gttatatatc ttgaccaagg
      721 tgctaatttt gaaggataaa aagcgtctcc agtacttcgt atttgaacaa ttattgcatt
      781 gtaattcagt tttttataag tttctaaaat ttcaataaaa tcggcttttt gtttgttaac
      841 agaatctgta cccgcttttg gccaatcaat atttactaca gttgctatcc aaacggctct
      901 gaattcattt ttcggatatt tggcatcata ttgtgaatga gcattccaaa atgaaagcag
      961 aactataaaa ttcaatacaa aaaaaggctt aattctaatc attatagtag tgttatgtaa
     1021 aaatcaaaag tagtttttta gagaagttta tcctataatt tttttaataa attctaattt
     1081 ttattgctgc aatttgtttg ccaaattagt tgaacactta ttttattttt aaatagttag
     1141 tgattattat tagattacac tttgaaaatg agcttctgtt tgtaaaaaat atataaaata
     1201 atagtccaaa aaagtacata caatagggca tatgccaatg aagcgtttaa aggatttaaa
     1261 aattgaggtg tgatgacata attataaaaa tactcattga tatttatttt ttcggatgca
     1321 atttctggat tttgaatttc aatcatcccc cagactctcg gcaatattcc tgacaaaaag
     1381 aatacaatca ttggattgac tccccaaatc aaaaaaagct tcgtccattt tttataattg
     1441 gcaatctcta tcaaataata taaaatcatt agaattgata tggctaagcc agctgtgtat
     1501 aaaacataag agctactcca aagtgatttg tttattggga aaaccaaact ccaaatcaaa
     1561 cccagtgcaa taagtgaaat accaagaacg atgagttttt ttgtgatttc tatttttgtg
     1621 gcttgtatat tcaataattg acctattaac attcctataa ttcctgttgc aatggcaggt
     1681 attgtgctta aaataccttc aggatcccaa gttttagtag cactccatag atgtccgttc
     1741 aagatagaat tgtctaacca agcggcaagg ttggttcctt tttcaaaatt tgctgcacca
     1801 aaaccgggta caggaataca agtcatcaaa aaccaataaa tcagcaataa tagaactgta
     1861 attccgattt ggatttttgg actagttttc aaaaatagga gtgaagtaaa gaaatatacg
     1921 attccaattc gttgcaaaac tccggggatt ctaacttctt gaaaactttc aattgagctg
     1981 taagctaaaa ataataatat ccccagaacc ccaaaagcca aataggtttt tattttttga
     2041 ttaaaatttc ccaaaagggc aattccaaca atagtagtaa ttaggattcg tattgtcagt
     2101 aatggaattc cctctaatcc taaaaagtgt atttttccaa aataatttaa aaacaatcct
     2161 aagcagaaaa ttcgaatgga acgaaccaca attttattaa aaatggcact gtcaaaatgt
     2221 ttgtcaggca ttgcaaaagg aattgctgtt cccataatga atatgaaaaa tggaaatacc
     2281 aaatcagtag gagtacagcc attccattca gcatgttcca aaggtggata aatgtgtgcc
     2341 caacttccgg gattattgac aatggtcatt accattattg taaatcctct gaaaacatca
     2401 agtgaaatta aacgttcttt tttcattgtt ttggagtttt taattcaaat tatgggttac
     2461 tatttcacaa tagcattttg tattacttgt tgaatgtctt ctctaatttt tcctttgact
     2521 aaagtattct ctacatattc aggatacgtt ctattagaaa gaaaaacata gatgatttgg
     2581 gtttcgggat cagcccaagc catatttcct gtaaaaccag tatgaccaaa actagcagga
     2641 gaaacacaac cgcaagttga gccgtctttt ccaattcgtt tgtcaaaacc taaacctcta
     2701 ttgactcctt ctgtgtaaaa ataacaagta ttaaaatcat taaatgtttt ttcggaaaaa
     2761 tagcgttggt tgccataact tcctttttgc aaatacaatt gcatcatttt ggctacatcc
     2821 attgcattcg aaaataatcc tgcatgacca gaaacgccac cttccatagc ggctcccata
     2881 tcatgaacat atccttgaag cgtttgatgg cggaaataat tgtctttttc agttggcgca
     2941 atcgagtttt tatcaaactt ttttaatgga ttgaataagg tataattcat tcctaatgat
     3001 ttatagaatt tttcctgaac caaaacatct aattttttat ggttcactct ttctaaatat
     3061 tcttttagaa gaatgaaggt caaatcacta tatttatact caattttttt gtccaatgga
     3121 ctgtcggcaa tgattttaat gatagtatcg tgatagtcat ttctgatgaa aatactatcg
     3181 gcaatttgtc taatatgctg ggcatcaaaa gttttttggt aatacttttt cgaaggcatt
     3241 ttggcactat ccaatgttgc tttgtagaaa ggaatccaag cttgaagacc tgcataatgc
     3301 gaaagcaaat ttttgaaacc aatatctttt ttattggatt tacttaaaga aggcaacatt
     3361 ttagatagtt tagtatccaa tgtcactttg ttttggtcgt ataattgcat cacattgggc
     3421 agtgtggcaa ccatttttgt aattgaggct aaatcatata aatcggaatt tgtgaccaat
     3481 ttttccttat cataagtctg gtaaccatac gatttttgat aaattacttt tccatttcta
     3541 gccaccaata cttgtgctcc aggagtcatt tttccatcga tggctttctt ggcatattca
     3601 tcaatttggt ttaaaatcat tggattcata ccttcgtttt cgggtgctgt gaatcccaaa
     3661 cgatctaatt tttgggtact taccccagta tttacagcaa agttagaatt gatagaaaca
     3721 ggtaattttc ccttggcttc gatggcacca aaaataagct cagccgatac aatttgtgaa
     3781 attgttgagt tttgatagga aactactaat ccttcaatct catcaaaatt agttataggc
     3841 aacaaagaat aaggttttgc aaaaacatct aaaattactt tattgttttt ggcaatttct
     3901 tgcagccaca acaattcttt ttctgttaaa tcgtgatttt tccaagccaa atctgatttg
     3961 tggaaaccaa taattacggt gtcgaaattt tttaatttta aagtaagact gtcaatactt
     4021 gaagctgtta cttcggtaat ttcagtatat ttttttaaag tcgtcagaaa agaactgttg
     4081 tcatcgtctc ccaattttac ataggctatt ttttgatcta atttttttat cggaagtaca
     4141 tcgcttttat tattcaagac cgtgattgca ttttcaaata attgatattg taaagcatca
     4201 ttttcggttt tgtttatgtc ttgatacaag tttgcaaggt caattggatt tggtttattt
     4261 aagcctgctt tgaatttgta atgcaaaatt ttctttactg aatgtgccaa tctttcctca
     4321 gaaattgttc cttgattgta agctatttta attttttcga ctgccagcgg aacattttct
     4381 ggacataata aaatatcatt tcctgccata aatgcggcca aatcgatatc tcctggttgc
     4441 ataaaattgc tcgcaccctt cattccaagc gcatcggtga aaattaaacc attaaatccc
     4501 aattcttttt gaagtaaatt agtaaccaca ttatacgaaa gggacgaagg acaatcttgt
     4561 ttcgtttcta aactcggaat attcaaatga gcaaccatta ccgaggccaa accatcgtca
     4621 aacattcttt tgaaaggata taattctact aaatctattc gttctttaga aaaattaatg
     4681 gttggtagcg ttttgtgaga atcagtagca gtatctccgt gaccaggaaa atgtttgcct
     4741 gtgcaaaaaa cgccttgact ttgctctcct tttaaaaacg caatggcttt atcggcaaca
     4801 ttgacttttg attctccaaa agagcgattt ccaataattg gatttttggg attggtattg
     4861 atgtcgagca caggagcaaa attgaaatga attcccattc gtttgcattc attccccata
     4921 tttgagccca ctttttcaat cagttttaaa tcttgaatag cgccaagagt catattccaa
     4981 ggatagcgat aagtcgaatc aagtcgcatt gccaaacccc attcggcatc aattgcaata
     5041 aacaaaggga cattggcttt cgattggaac ctattggtta attgagcttg acgaactggc
     5101 ccaccttgaa aaaaaataat gccacccact ttgtaaccct gaaccaaaga atcaattttc
     5161 ttggcgtgaa gactatcttt attagagtag gcagcaacca taaaaagttg tcctattttt
     5221 tcttccaaag acatttttgt ataaatgcta tctacccaat gattttcttc atccgaatct
     5281 ttaaaaaaag tagcgccaac ggttttattt ttagcaacat atatttcttt atttgctgaa
     5341 atcgtagttt cttttttggc atcaatcgtt ttttggtctt tttttaaacc tatacaattg
     5401 ctagccaaaa aggcaaaagt taaatagaaa gtaattttta taaagtaatt tttcattgtt
     5461 caatgaatta atttttgatt attatttgga ctaaaaatct attttttctg cttttttgct
     5521 gatggagtag agaccaataa aagtaattaa agcgtttatg ataatgagtt cattgtcaaa
     5581 tatatatcca aagaataatg ttgcggaatt ttcacttact aaataggtta ttgccggtga
     5641 caaaaagcag atataaggaa ccaatttgtc gtttactttt cgtcctttat gaaataatcc
     5701 gaaggcatat aaacctaaca aagggccata agtgtaggaa gcaaccttga aaatcattcc
     5761 aacaacagaa ctatcattta tagaattaaa gactaaaatt attagaaata ataaggctga
     5821 aaagcttaaa tggactaaat gtctagttct aatgatattt tttttggcct gattttcgga
     5881 tttttccatt cccaaaaaat ctacgcaaaa agaggttgtc aatgcagtta aagctgaatc
     5941 ggtagtggca aatgtagctg caataagtcc caataaaaat actattgcgg gtattactgt
     6001 taaatgattc aaggcaattt ctggaaataa taaatcggtt ctgggtttat tagtaatgga
     6061 atcaattggg atttcaattc cgtttttgga tgcataaatg tataataatg ctcccacgct
     6121 caaaaagaaa atattaatga ccacgaagat tccagtgaaa gtgaacatgt ttttttgggc
     6181 atcttttatg tttttgcagc tcaagttttt ttgcattaaa tcttggtcaa gacctaccat
     6241 tgctatggtt acaaacattc cgccaagaat ttgtttggca aaatgaaact tgttggtcat
     6301 aaaatcatca aagaaaaata tttgagaata attactatcc ttcacttcgt ggaatgcgcc
     6361 aaccaaactc aaatctaaac tattgcaaat gaaataaatt gttaaaaaca ctgaagaaac
     6421 tagaaagaat gtttgcaaag tatctgtgat aattattgtt ttaagtccgc cacgataagt
     6481 ataagaaaaa attaataata aagaaattaa gaccgttaac gaaaatggaa tgttataata
     6541 atcaaataca aaacgctgca aaacaataac cacaagatag agtctaaaag cagagctaat
     6601 ggttctgctt actaaaaaaa tagaagccgc agttcggtaa ctataatgcc ccattctttg
     6661 ctcgatatag ccataaatgg aagttaaatt cattcgataa tagagcggaa gaagaagttt
     6721 tgtaatgata ataaacccaa tagcatttcc caatacaaac tgaaaatatt tgaattgttc
     6781 cccatttggc gaacccactt ctcccggaac cgagatgaaa gttactccag aaagtgctgt
     6841 tccaatcatt ccaaaggcaa ctaaatacca tttagaattt ttattggctt tgaaaaaggc
     6901 atcatttcca tcatttttct tgctgacgct atgagaaatg tagaaaagta gtccaaaata
     6961 aacgatgatt aatataagga ttgcgcttgg agtcattttg tgttttttag ggttttaatt
     7021 aagctttatt tctagatagc aaaacagctc tcacactttt gtgttctttt aataattggg
     7081 cggctaattt ttcgtcaata tgaagtgctt ctactatcat ttcaacacct ctttgcacta
     7141 attttttatt tgacaattgc atatcaacca tcttattacc ttttactctt ccgagttgaa
     7201 tcattacaga agtcgtaatc atgtttaaaa ccattttttg cgatgttcca gctttcattc
     7261 gggtacttcc agtaagaaat tcgggaccaa ccaataattc tatcgggtaa tcggcttctt
     7321 tggatatttt actattattg ctgcaagtga tgctgcccgt tatgatgcta ttttcttgag
     7381 ctttttttaa tccgccaatt acgtagggag tatttcccga agctgcaatt ccaatgacta
     7441 catccaatga atttatatcg tgttttttta aatcaatcca agcttgctca aaatcatctt
     7501 cggcgttttc gaccgctttt cgaatggcaa aatctccacc agcaattatt ccaatgacca
     7561 tttgatcgct tactccaaag gtaggaggac actcggatgc gtctaatatt cctaatctac
     7621 ccgatgttcc tgcaccaatg taaaacaatc tcccacccaa tttcattttg gaaacaattg
     7681 catctaccaa tttttctata ttgggtattt gagtttcaac ggcatgagcc acttttttgt
     7741 cctcggcatt gatgttttcc aataactctt tggttgacat tttgtccaaa ttactataat
     7801 tggattcttt ttcggtatcg ggatatattt ttttcattga atttgtttag gtgtgttaga
     7861 aataagtttt tttgaattat tattttgaaa acaaatctat ttcaggatat taaaattagt
     7921 aaataagata ctttgccctt ttagttttga agtcttttaa tccttttttc cagctttttc
     7981 taattttatt ttcagattca ccattttcta tttgttgttg gagttgtttc gttccagcaa
     8041 gctttgtgaa aaatgcattg aaaaatttag ttttgtcggt ggtcatttta taagaacgaa
     8101 tcaaccattg cagttccaac cgattcactt ttttatgagt tgttaaatct tggccatagc
     8161 aaatttgacc gttataaaga ggttcttttg ccccaaaatt gggctttgga gtaaatgaaa
     8221 aagtgc
//
LOCUS       DAOSNN010000106         8220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8674_length_8220_cov_5.271525, whole genome shotgun sequence.
ACCESSION   DAOSNN010000106 DAOSNN010000000
VERSION     DAOSNN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8674_length_8220_cov_5.271525"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..2108)
                     /gene="glmS"
                     /locus_tag="PLQ70_06715"
     CDS             complement(261..2108)
                     /gene="glmS"
                     /locus_tag="PLQ70_06715"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQE34345.1"
                     /translation="MCGIVGYIGYREAYPIVIKGLKRLEYRGYDSAGVMLYDGNDIKL
                     SKTKGKVVDLEAKALKEISTNGTIGIGHTRWATHGVPNDVNSHPHVSNSGDLVIIHNG
                     IIENYAPLKVELIKRGYVFHSDTDTEVLVNLIEEVQKKENLKLGKAVQVALNQVFGAY
                     AIAVFDKKNPDEIVVARLGSPLAIGIGEGEYFIASDASPFIEYTSNAVYLEDGEMANI
                     RLHKPMKIRKIKDDSLVDPYIQELQMNLEQIEKGGYDHFMLKEIFEQPDVITDTYRGR
                     LHANEGIIQMSGVEDNLEKFLNAERIIIVACGTSWHAGLVAEYILEEFTRIPVEVEYA
                     SEFRYRNPIINSKDVVIAISQSGETADTMAAIKLAKEKGAFVYGVCNVVGSSISRETH
                     AGSHTHAGPEIGVASTKAFTTQITVLIMMALRLAKAKGTLSKTDFHRYLQELEIIPEK
                     VKEALETNDKAKEIATIFKNSHNCLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAE
                     MKHGPIALIDERMPVIVIAPKQGHYDKIVSNIQEIKSRSGIIIAVVTKGDTQVRGLAD
                     HIIEIPETSDALSPLITTIPLQLLSYHIAVLRGCNVDQPRNLAKSVTVE"
     gene            complement(2118..3887)
                     /locus_tag="PLQ70_06720"
     CDS             complement(2118..3887)
                     /locus_tag="PLQ70_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HQE34346.1"
                     /translation="MHNNYIFKSLLFLVSILLFVSCDKDYSAIGDALIGENHFELAKY
                     NSSVIAYNEKITPIQSSNLPINALGIYDNPAFGKTTANFVTQLSLVNVNPTFGPNAVI
                     DSVVVDVPYFSTLKSTNATTGNSEYELDSIYGEPKAKIKLSVYESNRYMGKQNGVDQI
                     FYTNQDSEFDSYKVGTRLNDHASKKEQNDEFFFNPRENRFVTPASADGKTAAVTTRTA
                     PSMRLNLNVEFFKNKILNAPAGSLTTNDVFINYFRGLYFKVEESGADKGALANINFAK
                     GTITIKYKEDTSTTDKTKVEKTLVINMSGSTANLLEQTNTNANYNTATSNPNQSTGDE
                     RLYLKGGEGSLAVLNLFNPADNYSYEVLKNSNKEPIDENGNVIPLQSNGDPKSGYFLI
                     YTKKNISNGVSDELEDIRYPLIDDTQGQIYHSIKRRWLINQANLVLNVDADNTEFKKS
                     YEPNRIYLYDYTNDTTVLDYYLAAATNDKNTSKYAFGGFLTNDATTKRGASYKINLTN
                     HIRNLIINSDAKNVKLAVVVTEDINVADFYKLKTSSGLFTQAPKASVMNPLGTILYGT
                     GATSTNLPENKKIKLEIYYTKTN"
     gene            complement(3908..4714)
                     /locus_tag="PLQ70_06725"
     CDS             complement(3908..4714)
                     /locus_tag="PLQ70_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQE34347.1"
                     /translation="MEDKRILYVSSEVVPYLAENEVSSMSYEVPKMINNQGGQIRIFM
                     PRYGNINERRHQLHEVIRLSGMNLVVNDLDMPLIIKVASIPKERIQVYFIDNDEYFKR
                     KATFTDEEGLMFPDNDERAIFFAKGVVETVKKLNWVPDIIHVHGWMAGMLPIYMKHFY
                     KNEALFSETKIVTSVYSQSFEGTLDIEMINKIKFDGVPNESISDLESPDYENIMKATI
                     NHSDAVIIASDNLSSSLTKFIESSGKPFLPFASKDKFAETYCNFYKNFVL"
     gene            4793..5671
                     /gene="panC"
                     /locus_tag="PLQ70_06730"
     CDS             4793..5671
                     /gene="panC"
                     /locus_tag="PLQ70_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQE34348.1"
                     /translation="MFANYFNKTPMHIFYGKAALIDFLKSIKTNQSSIGFVPTMGALH
                     QGHLSLMQQSISNNDITVVSIFVNPTQFNNPDDLAKYPRTLEEDVKKIATLNPEIIVF
                     APTTDDIYEGQTISQSFNFDGLENQMEGKFRPGHFNGVGTIVKRLFEIVEPTNAYFGE
                     KDFQQLQIVKKMVAKNKMNVTIVGCPIFREPNQLAMSSRNALLTPKEKQEASIIFKTL
                     IESKEKFQKYNAKIATDYVQKSFGNNPTFTLEYFQIADEATLLPCLRKNKNKNYRAFI
                     AVFVNNIRLIDTISLN"
     gene            5686..6036
                     /locus_tag="PLQ70_06735"
     CDS             5686..6036
                     /locus_tag="PLQ70_06735"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HQE34349.1"
                     /translation="MQIQVVKSKIHRVKVTGADLNYIGSITIDEALMEASNLIEGEKV
                     SIVNINNGERLETYAIKGEKNSGEITLNGPAARKVQKDDIIIIISYATLDFEEAKTFK
                     PWIIFPNEKDNSLT"
     gene            6209..7357
                     /locus_tag="PLQ70_06740"
     CDS             6209..7357
                     /locus_tag="PLQ70_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQE34350.1"
                     /translation="MKQLLLFLFFSIPVFSQKTTETFVSTQLGESRQITIGLPDSYEK
                     NPNKKYPILVLLDGDYLFDPFYGALNYGAYWEDMPETIIVGINQNTNEERIDDSNYDD
                     GNGLPSGKGANFFAFIGGELLPYIEKKYRVAPFRIIAGHDTTAGYLNFFLFKDYSVFN
                     AYISLSPELVPEMDTRIPERFAALKQPLFYYQSTGDGDIKDILESVNKLDTNIKTANN
                     PLINYKYDSFKGASHYSSVLHSIPNALYQIFDCYKPISMTEFSEKIVILPNDQTKYLT
                     DKYDLITKTLGLKIPVRLSDFKAIEAAILKNKSYSELEKLAEIAGKNYPKSMLSDYEL
                     GLMYEKNGDAKRAAKSYQKASQLEEIGDLTKDMMMEKFDEMQSLTTKK"
     gene            7360..>8220
                     /gene="radA"
                     /locus_tag="PLQ70_06745"
     CDS             7360..>8220
                     /gene="radA"
                     /locus_tag="PLQ70_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQE34351.1"
                     /translation="MSKVKTSFFCQNCGAQYSKWQGQCNSCKEWNTIAEEIIQKEEKV
                     AWKSESTSNSKAPKPLRINEIDSAEEIRMNTTDGELNRVLGGGIVPGSLILLGGEPGI
                     GKSTLLLQISLKLPYKTLYVSGEESQKQIKMRAERITPNGDNCYILTETKTQNIFKQI
                     EAIEPEIVIIDSIQTLHTDYIESTPGSISQIRETTAELIKFAKETNIPVILIGHITKD
                     GTIAGPKILEHMVDTVLQFEGDRNHVYRILRSHKNRFGSTAEIGIYEMLGSGLREVSN
                     PSEILISHKDE"
BASE COUNT         2741 a         1510 c         1267 g         2702 t
ORIGIN      
        1 ttttgtaagt tagtgtgacc aaagtacaaa tatttttgat attaataaaa aaacgttaaa
       61 ataatttaat aatatataat ttcactaaaa acagctattt tcaaaaacat taaattaatt
      121 taggctattt agttaaaatg atgtgattta ttatgcgtgc atataatgat agcattttga
      181 aaatagatat ttctcaaaaa aatgcataaa aaaagcgagg tgaaaaacct cgcttttaat
      241 gataatatat actatattac ttactcaaca gtcactgact ttgcaagatt tcggggctga
      301 tctacattac aacctctcaa aacagcaata tgataagaca ataattgtaa aggaatagtg
      361 gtaattaatg gcgataaagc atcggaagtc tcaggaatct ctatgatatg atctgccaat
      421 cctcttactt gagtatctcc ttttgtaact acagctataa tgataccact tctagattta
      481 atttcctgaa tattactaac aattttgtcg taatgtcctt gcttaggagc aataacaatt
      541 actggcatac gttcatctat caaggcaatt ggaccgtgtt tcatttccgc tgcaggatac
      601 ccttcggcat gaatatagga gatttctttg agttttagtg cgccttctaa tgcaacagga
      661 aaattataac ctcttcctaa atacaaacaa ttatgagaat ttttgaaaat tgtagcaatt
      721 tctttggcct tatcgttagt ctctaaggct tcttttactt tttcaggaat aatttctaat
      781 tcttgcaagt atctatgaaa atcggtttta gacaatgttc ctttagcttt ggctaaacgc
      841 aaggccatca ttattaaaac ggtaatttga gtagtgaatg ctttggtaga tgcaactcca
      901 atttctggac cagcatgggt atgtgaacca gcatgggttt ctctagaaat agatgaacca
      961 accacattac aaacaccata aacaaaggca cctttctctt tggccaattt tattgctgcc
     1021 attgtatcgg cagtttctcc agattgagaa atagcaataa caacgtcttt gctatttata
     1081 attggatttc tatatctaaa ctctgatgcg tattcaacct caacaggaat tcttgtaaac
     1141 tcctctaaaa tatattctgc aacaagacct gcatgccaag aagttccaca agctacaata
     1201 atgattcgct ctgcgttaag aaatttttct aaattatctt caacacccga catttgaata
     1261 atcccttcgt ttgcatgaag tcttccacga tacgtatcgg ttattacatc aggttgttcg
     1321 aatatttctt ttagcataaa gtggtcgtaa ccgccttttt ctatttgttc caaattcatt
     1381 tgaagctctt gaatataagg atcaactaaa gaatcgtctt taatttttct tattttcata
     1441 ggcttatgca atctgatatt agccatttct ccgtcttcta aatatactgc attagaagta
     1501 tattcgataa atggtgaggc atcagatgct ataaaatatt ctccttcgcc aattcctatt
     1561 gctaacggac ttcccaaacg ggcaacaaca atttcgtcag ggtttttttt gtcaaaaaca
     1621 gctattgcat aggccccaaa cacttgattt aaggctactt gaactgcttt tcctaatttt
     1681 aaattctcct ttttttgaac ttcttctata agattcacca gcacttcagt atcagtatct
     1741 gagtgaaaaa catagcctct ttttattaat tctactttta aaggagcata gttttcgata
     1801 attccattgt gaataatcac taaatctccc gagttcgaaa cgtgaggatg ggaattcaca
     1861 tcatttggca caccgtgagt tgcccaacga gtgtgtccta tgcctattgt tccgttagtt
     1921 gaaatttctt ttaaggcttt cgcttctagg tcaacaactt ttccttttgt tttagaaagc
     1981 tttatatcat ttccatcata cagcattacg cctgcactat catatcctct gtattccagt
     2041 ctttttaatc cttttattac aataggataa gcttctctat agccaatgta tccaacaatt
     2101 ccacacatag attgggttta attagttttt gtataataaa tctctagttt tatttttttg
     2161 ttctcgggca aattagttga agtagcaccc gttccataaa gtatagttcc caatggattc
     2221 attacgctag cttttggtgc ttgagtaaac agtccacttg atgttttcaa tttataaaaa
     2281 tctgcaacat tgatatcttc agtaacaact acagccaact tgacattttt tgcatcggaa
     2341 tttattatta aattcctaat atgattggta agattaattt tatatgaagc ccctctttta
     2401 gttgttgcat catttgttaa aaatccgcca aacgcatatt ttgaggtatt tttatcgttt
     2461 gttgctgctg ctaaataata atctaaaaca gtagtatcat tagtataatc atacaaataa
     2521 attctattgg gttcatagct ttttttaaat tctgtgttgt ctgcatcaac attcaaaacc
     2581 aaattagctt gattaattaa ccatctcctt ttaatggagt gataaatttg accttgagta
     2641 tcatcaatta atggatatct aatgtcttct aattcatcag aaactccatt tgaaatgttt
     2701 ttcttggtgt aaattaaaaa ataaccagat tttggatctc cattagattg aagaggaata
     2761 acattcccat tttcgtcgat tggttctttg tttgaatttt taagaacttc ataactataa
     2821 ttatctgctg gattaaaaag gttcagtact gctaacgaac cttcgcctcc ttttaaatac
     2881 aatctttcgt caccagtact ttggtttgga ttgcttgtgg ctgtattata attggcattt
     2941 gtattggttt gttccagtag atttgctgtg ctacctgaca tattaatgac taaagttttt
     3001 tcaacttttg ttttatcagt cgtagaagta tcttctttat atttaatagt gattgtccct
     3061 tttgcaaaat tgatattggc cagagcgcct ttatctgcac ctgattcttc tactttaaaa
     3121 tacaagcctc taaaataatt gataaaaaca tcgttggtag tcaaacttcc tgctggagca
     3181 ttgagtattt tatttttaaa aaactcaaca ttcaaattca aacgcatact aggagcggtt
     3241 cgggtagtca ccgcggcagt tttaccatcg gctgaggctg gagtaacaaa tctattctct
     3301 ctagggttaa agaaaaactc gtcgttttgt tccttttttg aagcatgatc attcaagcgt
     3361 gttccaactt tataactatc aaattctgaa tcttgattgg tataaaaaat ctgatctact
     3421 ccgttttgtt ttcccatata acgattcgat tcatatacac tcaattttat tttggccttt
     3481 ggctctccat aaatagagtc taattcatac tcactattac ctgttgttgc attagtcgat
     3541 tttaatgtac taaaatacgg gacatcaaca acaacactat caattactgc attaggtcca
     3601 aaagttggat taacattaac taatgatagt tgtgtaacaa aattggccgt tgtttttcca
     3661 aaagcaggat tgtcatatat ccccaaagca tttatgggaa gattacttga ttgaataggg
     3721 gtgatttttt cgttataagc tattacactt gagttatatt tagccaattc gaagtgattt
     3781 tcaccaatta atgcatcacc tattgcactg taatctttgt cacacgaaac gaaaagcagg
     3841 atactaacaa gaaataatag agacttgaaa atgtaattat tgtgcatgtt tacttgagaa
     3901 tgttaattta tagaacaaaa tttttataga aattacaata cgtttcagcg aatttatctt
     3961 tcgaggcgaa aggtaaaaaa ggttttcctg aagattctat aaattttgtt aaacttgaag
     4021 acaagttgtc tgaagcaata attacggcat ccgaatgatt gatagtagcc ttcataatat
     4081 tttcgtaatc tggcgattca agatctgaaa tagattcgtt tggaacacca tcaaacttaa
     4141 ttttattaat catttctata tctaatgtgc cttcaaaaga ctgactgtaa accgatgtta
     4201 cgatttttgt ttctgaaaat aaagcttcat ttttataaaa atgcttcata taaataggca
     4261 acattccagc catccaacca tgaacatgaa taatatctgg aacccaattt aattttttca
     4321 cagtttcgac aactcccttg gcaaagaaaa tggctcgttc atcattatca ggaaacatca
     4381 agccttcttc gtccgtgaat gtcgcttttc gtttaaaata ttcatcatta tcaataaaat
     4441 aaacctgaat tctttctttt ggaatcgaag ccacctttat aatcaagggc atatccaagt
     4501 catttacaac taaattcata cctgaaaggc gaattacttc gtgcaattga tgtcttcttt
     4561 cgtttatatt tccatatctc ggcatgaaaa ttctgatttg tccgccttga ttattaatca
     4621 tttttgggac ttcgtaagac attgaagaaa cctcattttc agctagataa ggcaccactt
     4681 cagatgatac atataatatc ctcttatctt ccatattgta aattacttaa tttattggca
     4741 ataaaaacca tgcaaaatta caaaaattta cgcagttctc gactaaaata ttatgtttgc
     4801 aaattatttc aataaaacac ctatgcatat tttctacgga aaagctgctt taatagactt
     4861 tttaaaatct attaaaacaa atcagtcctc gataggcttt gtacccacaa tgggtgcttt
     4921 acaccaaggt catttgtcat tgatgcaaca atcaatatca aataatgaca ttactgtggt
     4981 gagtattttt gtgaatccca cacaatttaa taatcccgat gacttagcaa aatatccgag
     5041 aacattagaa gaagacgtca aaaaaatagc tactttaaac cccgaaatta ttgtttttgc
     5101 cccaacaacc gatgatattt atgaaggaca aaccatttct caatctttca attttgacgg
     5161 tttagaaaat caaatggaag gaaaattcag accaggtcat ttcaacggtg tgggcaccat
     5221 tgtaaaacgt ctttttgaaa ttgtcgagcc cacaaacgct tattttggcg aaaaagactt
     5281 tcaacaattg caaattgtaa aaaagatggt tgccaaaaac aaaatgaatg ttactattgt
     5341 tggttgtcca atatttagag aaccaaacca actggcaatg agttctagaa atgctctttt
     5401 aactccaaaa gaaaaacaag aagcttcaat tatttttaaa acattaattg aatctaaaga
     5461 aaaatttcaa aaatataacg ccaaaatcgc aactgattat gttcaaaaat cattcggaaa
     5521 taaccctact tttactttag aatattttca aattgccgat gaagcaactc ttttgccttg
     5581 tttaagaaaa aataaaaaca agaattaccg tgcttttata gccgtatttg ttaataatat
     5641 ccgattgatt gataccattt cattaaatta atttactttt gcgacatgca aatacaagta
     5701 gtaaaatcaa aaatacaccg tgtaaaagtc actggagccg atttaaatta tatcggcagc
     5761 atcactattg acgaagcatt aatggaggct tcaaacctta tcgaaggcga aaaagtatcc
     5821 attgtaaaca tcaataatgg cgaacgactt gaaacttatg ccatcaaagg cgagaaaaat
     5881 tcgggcgaaa taactcttaa tggtcccgct gccagaaaag tacaaaaaga cgatatcatt
     5941 attatcattt cctatgcaac tttagatttt gaagaagcca aaaccttcaa gccttggatt
     6001 attttcccca atgaaaagga caattcgttg acataaaaac cctttatcta tccacctgtt
     6061 ttcaatcctt ttcttgtttg aatattttgg atatattcgc tttttagata gtttactttt
     6121 taaaaaagca actaaaaaaa tagtatattg ctgctctttt cgacccctaa aaattcgaaa
     6181 gaaattaatt tcctaaaccc cagaaattat gaaacagtta ctactatttt tgtttttttc
     6241 aatccctgtt ttttcgcaaa aaacgaccga aacattcgtt tcaacccaat taggcgaaag
     6301 cagacaaatc actattggac tacccgattc ctacgaaaaa aacccaaata aaaaatatcc
     6361 aatactcgtt ttgcttgacg gcgattactt gttcgacccg ttttatggcg ctttaaatta
     6421 tggtgcctat tgggaagata tgcccgaaac tatcatcgtt ggaattaacc aaaacacaaa
     6481 tgaagaacga atagacgata gtaattatga tgatggaaac ggcttacctt ctggaaaagg
     6541 cgcaaatttc ttcgctttta ttggtggtga attgcttcct tatattgaaa aaaaataccg
     6601 tgttgctccc tttagaatca ttgccggaca cgacacaaca gcaggttatt tgaacttttt
     6661 cctttttaaa gattattcgg tatttaatgc ctacatttca ttaagcccag aacttgtgcc
     6721 tgaaatggat acccgaattc cagaacgatt tgcggcatta aaacagcctc ttttctacta
     6781 tcaatctact ggcgacggcg acataaaaga cattttagaa tcggtaaata aactcgatac
     6841 aaatatcaaa accgccaaca atcctttgat taattacaaa tatgactcct tcaaaggtgc
     6901 ctctcattac tcttcggttt tgcattccat accaaatgct ttgtaccaaa ttttcgactg
     6961 ttacaaaccc atttctatga ccgaattttc agaaaaaatt gtaattcttc caaatgacca
     7021 aaccaagtat ttaactgaca aatatgactt aatcacaaaa acattaggtc ttaaaattcc
     7081 tgtgcgacta tccgatttta aagccataga agctgcaatt ctaaagaaca aatcctattc
     7141 tgaattagaa aaattagctg aaattgcagg aaaaaattac ccgaaatcaa tgctgtccga
     7201 ttatgaatta ggattaatgt atgaaaaaaa tggagatgca aaacgagcag caaaaagcta
     7261 tcaaaaagct tcgcaattag aagaaattgg cgatttaaca aaagatatga tgatggaaaa
     7321 atttgatgaa atgcaaagtc taaccacaaa aaaataagaa tgtctaaagt aaaaacttct
     7381 tttttttgcc agaattgtgg cgctcaatat tcgaaatggc aaggccaatg caattcttgt
     7441 aaagaatgga acacgatagc cgaggaaatt attcaaaaag aggaaaaagt agcttggaaa
     7501 agcgaatcga cttctaattc caaagcacca aaacccttgc gaataaacga aattgattct
     7561 gccgaggaaa tccgaatgaa caccaccgac ggcgaactca atcgtgtatt gggtggagga
     7621 attgttccag gttcattaat ccttttgggt ggcgaacctg gcatcgggaa aagcacgctt
     7681 ctgctccaaa tttctttaaa attaccttat aaaaccttat acgtttcggg cgaagaaagc
     7741 caaaaacaaa ttaaaatgcg tgccgaaaga atcacaccaa acggagacaa ttgctacatt
     7801 ctaaccgaaa ccaaaaccca aaatatcttc aaacaaattg aagcgattga acccgaaatc
     7861 gtgattatcg attccatcca gacccttcat accgattata tcgaatcgac tccgggaagt
     7921 atttcgcaaa ttcgagaaac gactgccgaa ttgatcaaat ttgccaaaga aaccaatatt
     7981 ccagtaattt taattggtca tattaccaaa gacggaacta ttgctggacc caaaattttg
     8041 gaacatatgg tcgatacggt tttgcagttt gaaggcgaca gaaatcacgt atatcgcatt
     8101 ttgcgttcgc acaaaaaccg ttttggctct accgccgaaa tcggaattta tgaaatgcta
     8161 ggcagcggtt tacgagaagt ttcgaaccct tccgagatat tgatttcgca caaagacgaa
//
LOCUS       DAOSNN010000107         8085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8882_length_8085_cov_4.727024, whole genome shotgun sequence.
ACCESSION   DAOSNN010000107 DAOSNN010000000
VERSION     DAOSNN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8882_length_8085_cov_4.727024"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PLQ70_06750"
     CDS             complement(<1..1161)
                     /locus_tag="PLQ70_06750"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQE34352.1"
                     /translation="MKTINDFDFKNKKAIIRVDFNVPLDENFNVTDATRIEAAKPTID
                     KIIADGGSVILMSHLGRPKGVDRKYSLGHIIKKASEILGQVVYFAPDCIGSEAEKAVS
                     NLQPGQILLLENLRFYKEEEAGDVEFAKELASFGDVYVNDAFGTAHRAHASTTIIAQF
                     FPEAKCFGLLLAREIESLNKVLKDSVKPVTAVLGGSKVSSKITVIENILDKVDHMIIG
                     GGMTFTFIKALGGKVGNSICEDDKQELALEILRLAKEKGVQIHIPVDVVAADDFSNTA
                     NTQVVDVREIPDGWEGLDAGPKSLENFKKVILESKTILWNGPLGVFEMESFAKGTIAL
                     GDFIAESTANGAFSLVGGGDSVAAVKQFGFEDKVSYVSTGGGAMLEMLEGRIL"
     gene            1387..1539
                     /locus_tag="PLQ70_06755"
     CDS             1387..1539
                     /locus_tag="PLQ70_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34353.1"
                     /translation="MIHTLLNDLDALYVVIFLFITMLTAIWIGYKIGFKKAKVDKKTP
                     KFLHPY"
     gene            1557..2153
                     /locus_tag="PLQ70_06760"
     CDS             1557..2153
                     /locus_tag="PLQ70_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34354.1"
                     /translation="MGFTFAMAGSRYENRKNNLIDEANCIGTAVLRANIYPDSLKNEF
                     KKDFKEYLKSRKAYYLLENDEEKLNASLKQSAMVSKKLWSRATFYAKNKDYFIQSNMM
                     VPALNDMFDSASKSNMVLNSRVPETIVYLMFSFSIIISFFIGYNSGLEKKINIKFVTG
                     FTFLICVVIFITLDLDRPRRGLITLDSEISLLEKLNQP"
     gene            complement(2271..3149)
                     /locus_tag="PLQ70_06765"
     CDS             complement(2271..3149)
                     /locus_tag="PLQ70_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQE34355.1"
                     /translation="MLNAIFKHSFSLLHIDYVKLNQNWNYNNVISPYYRLYYIDEGMG
                     TVSDNSNRLILEPGYLYLIPSFTLCNLNCEAYLSQYFIQFFEESPDGISLFTNNRLVM
                     KLATTAIDIILIKRLLETNPGRGINRSDNPKVYEKEAYYKEYRLLNEAMKTHRKFENQ
                     GILLQLISRFLSSNGFKSSASQSVPSKILDTMSYVQLHLGENLTVTKLAQRVNQNQDY
                     FSKQFLLHTGQRPLNYIHEKKIERAQYLIATTNKSFLEIALDTGFSNLPHFSKIFKQI
                     VSLTPREYRQQNSHLK"
     gene            3348..5318
                     /locus_tag="PLQ70_06770"
     CDS             3348..5318
                     /locus_tag="PLQ70_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HQE34356.1"
                     /translation="MFYKIQKQISLKSIFLFLLLCALKSNAQQIINSPDNKLKVQISV
                     LNGKPTYTVFYNEKVVLEQSPLGLKTNVGDFSNGLELSTKIIPTKIDESYELPNIKQS
                     KVHYIANETSFSFTKENKPAIDIIFRVSNNDVAFKYKVHPQNKALSAVVKEEASGFVL
                     PAETTTFLSAQSNAMVGYARTMPSYEIPYTVDAPMGENGIGNGYTFPCLFKLKDSKGW
                     ILISETGIDSQYCASRLIGRENGLYTIGFPMEGENNGIGSATPGIALPSETPWRTITV
                     GETLAPIVETTVPFDLVKPRYEASQKYEYTKGTWSWIIGMDKSMNYNEQIRYVDFSAD
                     LGYKTLLVDALWDTQLGREKTETLAKYAANKGVGLYLWYNSNGSWNDAPQTPRGIMNN
                     SIARRKEMAWMKKIGIKGIKVDFFGGDKQETMKLYEDILFDANDYGIMVIFHGTTLPR
                     GWERMYPNYVSSEAVLASENLFFTQKSCDTEAFSATLHCFIRNTVGSMDFGGSALNKF
                     YNDKNIPNKGSKRMTSDVYALATAVLFQSGVQHFALAPNNLTDAPKWAIDFMKEVPTT
                     WDEVRFIDGYPGKYAIIARRNGNKWYIAGINAQKETIKTKIKLGMIPMGDKLKKYSDD
                     TLLNGSVSDLIQKKNQEIELIIPCNGAVLITN"
     gene            5348..6991
                     /locus_tag="PLQ70_06775"
     CDS             5348..6991
                     /locus_tag="PLQ70_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HQE34357.1"
                     /translation="MNRRNALKLGVSALAGLYLSPLLGQSKILETPAFKGTFEPTWDS
                     LAKYQTPTWFRDAKFGMWAHWGPQCEPEAGDWYARGMYQEGSRQYKFHLEKYGHPSKF
                     GFKDVINVWKADKWNPEELVNLYKDSGAKYFMAMANHHDNLDLYDSKFQPHWNSTKIG
                     PNKDIIGGWEKAARKAGLPFAVSIHAAHAWTWYESAQGSDKEGALAGVPYDGKLTKAD
                     GKGTWWEGLDPQELYAQNHPLSAVSSDRGSIHKQWDWAEGSSIPSTAYCENFHDRTID
                     LIDKYNPDMIYFDDTALPLWPVSDAGLRITAHLYNKSLEKNKTVQTVVTGKILNEQQR
                     KAIVWDIEKGQSNSIEPLPWQTDTCIGNWHYDRGIYNNKRYKSAKTVIHTLIDVVSKN
                     GNLMLNIPVRGDGSIDELEREIVKEIGVWMKLNSKSIYGTRPWKIFGDGPQQESAGAL
                     TAQGFNEGKGKPYTSEDIRFAQKDKKLYATVMAWPENGTAIIKSLGKSSPHYTEKIKQ
                     IQLVSTGEKLKFKQHSDALEVYFPNQKPEASYANALEII"
     gene            7002..7343
                     /locus_tag="PLQ70_06780"
     CDS             7002..7343
                     /locus_tag="PLQ70_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HQE34358.1"
                     /translation="MVSKKICYACDLIDNPELIEKYKNYHSKDNAWPEITKSIKDSGI
                     IDMEIYLFANRLFMIMEVNETYTPERKQEMDAANPKVQEWEQLMWQFQQPLPGAKEGE
                     KWLEMERIYKL"
     gene            complement(7427..>8085)
                     /locus_tag="PLQ70_06785"
     CDS             complement(7427..>8085)
                     /locus_tag="PLQ70_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HQE34359.1"
                     /translation="IILDYYSKQYLSFGMSFNINTFSIDIAKLSPTDPDYGITNDRRT
                     TNNNFDVGFLYRWDQFYLSVNGANILGKNIDQFSGYKIEPNLLLNYQVYTGYVIKTAN
                     YSEIEPSAFFQYFASDGRSSTDLNFKYRKYNNRDEYYWIGASYRFLNDQVLKPLNLGP
                     MFGFKKSVFYFGYSYQMTFNEMASFNSGTHMITIGLDFLQGISDCPCTQSVDHHGWGY
                     "
BASE COUNT         2767 a         1406 c         1438 g         2474 t
ORIGIN      
        1 taaaatccta ccttctaaca tctctaacat ggctcctccg cctgtagaaa cataacttac
       61 tttgtcttca aatccaaatt gtttcacggc tgcaactgaa tctccaccac ctacaagtga
      121 gaaagctccg tttgcagttg actcagcaat aaagtcgcca agtgcaatgg ttcctttagc
      181 aaaactttcc atttcaaaaa ctcccaaagg accattccaa agaattgttt ttgattctag
      241 aattactttt ttgaagtttt ccaatgattt tggaccagca tccaatcctt cccatccatc
      301 aggaatttca cgaacatcaa caacttgtgt gttggcagta ttcgaaaaat catcggctgc
      361 aacaacatcc acaggaatgt gaatttgaac tcctttttct ttggccaatc tcaaaatttc
      421 taaggctaat tcttgtttgt cgtcttcaca aatagaatta cctacttttc ctcccaaagc
      481 tttaatgaaa gtaaaagtca tccctccgcc aataatcata tgatctactt tgtccaaaat
      541 gttttcgata acggtgattt tcgacgatac ttttgaacca ccaagaactg ctgtaacggg
      601 ttttacagaa tcttttaaaa ctttgtttaa actttcgatt tctcttgcta acaataatcc
      661 gaaacattta gcttctggaa aaaactgtgc aattatagtt gtcgaagcgt gcgctctgtg
      721 tgctgttccg aaggcatcgt ttacataaac atctccaaat gaagccaatt cttttgcaaa
      781 ttcaacatct ccagcttctt cttctttgta aaaacgaagg ttttctaaca ataaaatttg
      841 tcctggttgt aaattactta ccgctttctc agcttcgctt cctatacaat ctggtgcaaa
      901 ataaactact tgacccaaaa tttcagaagc ttttttgatg atatgcccca atgaatattt
      961 tctatctact ccttttggtc ttcctaagtg agacatcaaa atcacacttc caccatcggc
     1021 aataattttg tcaatagttg gctttgcagc ttcaatacga gtagcatcgg ttacattaaa
     1081 attttcgtcc aaaggaacgt taaaatcaac acgaattatg gcttttttgt ttttgaaatc
     1141 gaaatcgtta atagttttca tcagatatta tttttattat ttattattta ttttgaaaga
     1201 aaaacaaatg tataacattt caagaactta aaaataagaa tagtttaaaa attagaagga
     1261 catattagcg aaaacgttgt aaaaatttaa atttattcca tttttgattg cctttcaaaa
     1321 aatccataca tttattgaat ctcattttaa aaaaacatta gttttaactt cgtaaaaaaa
     1381 tctataatga tacatactct tttaaatgat ttagatgctc tatatgttgt gatttttttg
     1441 tttattacaa tgttgacggc tatttggatt ggatacaaaa taggctttaa aaaagcaaaa
     1501 gtcgataaaa aaactccgaa atttcttcat ccttattagg gttgttggct ttaattttgg
     1561 ggtttacatt tgcaatggct ggttctcgtt atgagaatag aaaaaacaat ttaattgatg
     1621 aagctaattg tattggaaca gcagttttga gagctaatat ttatcctgat agtttaaaaa
     1681 atgaattcaa aaaagacttt aaagagtatt taaaatcacg aaaagcatac tatcttttag
     1741 aaaatgatga agaaaaattg aatgcttcat taaaacaatc ggcaatggta agtaaaaaat
     1801 tatggagccg agccactttt tatgcaaaaa acaaagacta ctttattcaa tcgaatatga
     1861 tggtacctgc gcttaatgac atgtttgata gtgcctcaaa atcgaatatg gttttgaatt
     1921 caagagttcc agaaactatc gtctatttaa tgttttcttt ttcgataata atttcctttt
     1981 ttattggata taattcaggt ttagagaaaa aaataaatat caaattcgta actggtttta
     2041 cttttcttat ctgtgtcgtg atttttatca ctttagattt agaccgacca agacgcggtt
     2101 tgattactct agactctgaa atttctttgt tggaaaaatt aaatcaacca taaaagtgat
     2161 aattaattat tttaaataat gagacctata catttctcat aaacctgaaa aaaaaaattg
     2221 acattccatt tttgtccaca gcaaaatatt gccgacgaca aaaaaaatca ttatttcaaa
     2281 tggctgttct gttgacggta ttctcttgga gttaagctaa caatttgctt gaatattttt
     2341 gaaaaatgag gcaaattaga aaaacctgta tccaaagcaa tttctaaaaa actcttgttg
     2401 gttgttgcaa taaggtattg cgcccgttcg attttctttt cgtgaatgta attcaaaggt
     2461 ctttgcccag tatgcagtag aaattgtttt gaaaaataat cctgattctg attcactctt
     2521 tgagccagtt ttgttaccgt gagattttcg cccaaatgaa gttgtacata actcattgtg
     2581 tctaaaattt tggaaggcac cgattgtgaa gcactagatt taaaaccatt agaactcaaa
     2641 aaccgggaaa tgagctgcaa taaaatccct tggttttcaa actttctatg tgttttcatt
     2701 gcctcattca gcaacctata ttccttataa taagcctctt tttcatagac ttttggatta
     2761 tctgaccgat taattcctct tcctggatta gtctccagca atcttttaat aaggatgatg
     2821 tcaattgctg tagtggctaa cttcatcacc aacctgttgt tagtaaacaa agaaatacca
     2881 tcgggagatt cttcaaaaaa ttgaataaaa tactggctca aataggcttc acaattcaaa
     2941 ttgcacaagg taaaacttgg aattagatac agataaccag gttctaatat caaacgattt
     3001 gagttgtcag aaacagtgcc cattccttca tcaatataat acaacctata atatggactg
     3061 ataacattgt tataattcca attctgattg agtttcacat aatcgatatg taataaggaa
     3121 aaggagtgtt tgaaaatagc atttaacatt ctgttatttt taagtaaaag tcggttttga
     3181 ataataaaaa atcggttttg aataaaaaat tagccctgta actgatttac atttgttttt
     3241 ttaattcaac gaatttgatt tgtaaaaata cgaatttgta aattttaaag cctaataata
     3301 gtatttttga tttttaaaat tcttaataca attaaaatat gaaaccgatg ttttacaaaa
     3361 ttcaaaaaca aattagtcta aaatcaatat ttttattttt gctgctttgt gctctcaaaa
     3421 gcaatgcgca acaaataata aatagtcccg ataataaact caaagtacaa atatcggtat
     3481 tgaatggaaa accaacttat acggttttct ataacgaaaa agtagtttta gaacaatcgc
     3541 cacttggatt aaaaacaaat gtgggcgatt ttagtaacgg attagaatta agtactaaaa
     3601 ttattccaac taaaattgat gaaagctatg aattgcctaa catcaaacaa agcaaagtgc
     3661 attatatagc caatgaaact tctttttctt tcaccaaaga aaataaacct gccatagaca
     3721 ttatttttag agtaagcaat aatgatgtag cttttaaata caaggttcat ccgcaaaaca
     3781 aagccctttc ggctgtggtc aaagaagaag cttctggatt tgtattacct gcagaaacta
     3841 ccacttttct ttcagctcaa agtaatgcaa tggtaggtta tgcccgaaca atgcctagtt
     3901 atgaaattcc ttatacagtt gatgcaccaa tgggcgaaaa cggcattggt aacggttata
     3961 cttttccttg tctttttaaa ttgaaagaca gcaaaggttg gattttaatt tcggaaacag
     4021 gcattgatag ccagtattgc gccagcagat taatagggcg tgaaaatggt ttatacacta
     4081 tcggttttcc aatggaaggc gaaaacaacg gaatcggttc agccactccc ggaatcgctt
     4141 tgcctagcga aactccttgg cgtactatta ctgtgggcga aactttggct ccaattgtcg
     4201 aaactactgt tccgtttgac ttggttaaac ctcgttacga ggcttcgcaa aaatacgaat
     4261 acaccaaagg tacttggagc tggattattg gtatggacaa aagcatgaac tataacgaac
     4321 aaatcagata cgttgatttt agtgctgatt tgggttacaa aacacttttg gttgatgcgc
     4381 tttgggacac tcaactaggg cgtgaaaaaa ccgaaacatt agcaaaatat gccgccaaca
     4441 aaggtgttgg tttgtattta tggtataatt ctaacggaag ctggaacgat gcgccacaaa
     4501 ctccaagagg cattatgaac aacagtattg ccagacgcaa agaaatggct tggatgaaaa
     4561 aaatcggaat caaaggaatc aaagtcgatt ttttcggtgg cgacaaacaa gaaacgatga
     4621 agttgtatga agacatttta ttcgatgcca acgattatgg aattatggtc atttttcacg
     4681 gtacgacctt gcctcgtggt tgggaaagaa tgtatcccaa ttatgtttcc agcgaagcag
     4741 ttttggcgag cgaaaatttg ttttttacac aaaaaagctg cgacaccgaa gcctttagtg
     4801 ccacattgca ttgttttatt cgcaatactg taggaagtat ggattttgga ggaagtgcgc
     4861 tgaataaatt ctacaacgac aaaaatattc ccaacaaagg ttccaaaaga atgacttcgg
     4921 atgtatatgc tttggctaca gcagttttgt ttcaaagtgg cgtacagcat tttgcattgg
     4981 ctcccaataa tttgaccgat gctccaaaat gggcaatcga ttttatgaaa gaagtaccaa
     5041 ctacttggga tgaagttcgt tttattgatg gttatcctgg taaatacgcc atcatagcga
     5101 gacgtaatgg aaacaaatgg tacatagcag gaataaatgc tcaaaaagaa accattaaaa
     5161 ccaaaataaa attagggatg attccgatgg gtgacaaact aaaaaaatac agcgatgaca
     5221 ctcttttgaa tggttcggta agcgatttga ttcagaaaaa aaatcaagaa atagagctaa
     5281 taattccttg taatggggca gttcttatta cgaattaaca gctcattaat ctgttaaaat
     5341 taagaaaatg aatagacgta acgcattaaa actaggtgta tcggcattag ctgggcttta
     5401 cttatctcct ttattaggac aatccaaaat tcttgaaact cctgctttca aaggcacgtt
     5461 tgaacccact tgggattcct tggcaaaata ccaaacacct acttggttca gggatgctaa
     5521 atttggaatg tgggcacatt ggggaccgca atgcgaacca gaagctggcg attggtatgc
     5581 tcgtggaatg tatcaagaag gttcacgtca atacaaattt catttagaaa aatacgggca
     5641 tccatcgaaa tttggattta aagatgtgat caatgtctgg aaagccgaca aatggaaccc
     5701 agaggaatta gtcaatttat ataaagattc gggagccaaa tattttatgg caatggccaa
     5761 tcatcacgat aatttagatt tgtatgatag caagtttcaa ccgcattgga attcgaccaa
     5821 aataggccct aataaagaca ttattggcgg ttgggaaaaa gcggctcgaa aagcaggatt
     5881 gccttttgca gttagtattc acgctgctca cgcttggact tggtatgaaa gtgcacaagg
     5941 ctcagacaaa gaaggtgctt tggctggagt gccttatgat ggaaaactga ccaaagccga
     6001 cggaaaagga acttggtggg agggtttaga cccacaagaa ttgtatgctc aaaatcatcc
     6061 gttaagtgcg gtttcttcag acagaggatc cattcacaaa caatgggact gggcagaagg
     6121 atcttcaata ccatcgactg cctattgcga aaattttcac gatagaacca tcgatttgat
     6181 tgacaaatac aatcccgata tgatttattt tgatgatact gccttgccgc tctggcctgt
     6241 tagcgatgca ggtttgcgca taacggcaca tttgtacaat aaaagtcttg aaaaaaataa
     6301 gaccgttcaa acagttgtta ctggaaaaat tcttaatgag caacaacgca aggcgatagt
     6361 ttgggacatc gaaaaaggac aaagcaacag catcgaaccc ttgccttggc aaaccgatac
     6421 ctgtattgga aactggcatt acgacagagg catttacaat aataaacgct acaaatcggc
     6481 caaaacagtg attcatactt taattgatgt ggtgagcaaa aacggaaatt tgatgctcaa
     6541 tattccggtt cgaggagacg gaagcatcga cgaattggaa cgagaaatcg taaaagaaat
     6601 cggtgtttgg atgaaactaa acagcaaaag tatttatgga acccgacctt ggaaaatatt
     6661 tggcgatggc cctcaacaag aaagtgctgg agcattaacc gcccaaggtt tcaatgaagg
     6721 aaaaggaaaa ccctatactt cagaagacat tcgttttgcg caaaaggaca aaaagcttta
     6781 tgccactgta atggcttggc cagaaaacgg cactgcaatt attaaaagtt taggaaaatc
     6841 atctccacat tatacagaga aaataaaaca gattcaatta gtgagtacag gcgagaaact
     6901 gaaattcaaa cagcattcag acgctcttga agtctatttc ccaaatcaaa aaccggaagc
     6961 ttcttatgct aatgcattag aaattattta attttaaaat tatggtttca aaaaaaatat
     7021 gttacgcctg cgatttaata gacaatccag aattgataga aaagtataaa aactatcatt
     7081 caaaagacaa tgcttggcct gaaatcacta aaagcatcaa agattcaggt attattgata
     7141 tggaaatcta tctcttcgct aaccgcttgt ttatgattat ggaagtcaat gaaacctata
     7201 ctcccgaacg caaacaagaa atggatgccg ccaaccctaa agttcaggaa tgggaacaat
     7261 tgatgtggca atttcagcag ccacttccag gagctaaaga gggcgaaaaa tggttagaaa
     7321 tggagcggat ttataaattg taattattgc cacacgaaag gattcatgcg agagcgggga
     7381 aattattgta tatctgagcg attgtattta tctctccatc cctcttttaa tacccccaac
     7441 catgatgatc tacagattga gtacaaggac agtcgctaat tccttgaagg aaatccaaac
     7501 caattgtaat catatgtgtt cctgaattaa aggaagccat ttcattaaaa gtcatttgat
     7561 aggaatatcc aaaataaaag accgacttct tgaacccaaa cattgggcct aaattcaagg
     7621 gttttaagac ctgatcgtta agaaaccgat aggatgctcc tatccaataa tattcgtctc
     7681 tgttgttgta ttttcgatat ttaaaattca aatcggtact cgaacggcca tcactagcaa
     7741 agtattgaaa aaatgccgaa ggttcaattt cggagtagtt cgcagttttg ataacatatc
     7801 cggtataaac ttgataattt aaaagcaaat taggttctat tttatatcct gaaaactggt
     7861 cgatgttttt acctaaaata ttagctccat ttacgcttag gtaaaattga tcccaacgat
     7921 ataaaaagcc aacatcgaag ttgttgtttg tagttctcct gtcgtttgta atgccgtaat
     7981 caggatcagt agggcttaat tttgcgatat caatactaaa agtattgatg ttaaaagaca
     8041 ttccaaaaga caaatactgt ttggaataat aatccaatat gatat
//
LOCUS       DAOSNN010000108         8061 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_8919_length_8061_cov_5.760305, whole genome shotgun sequence.
ACCESSION   DAOSNN010000108 DAOSNN010000000
VERSION     DAOSNN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8919_length_8061_cov_5.760305"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PLQ70_06790"
     CDS             complement(<1..1042)
                     /locus_tag="PLQ70_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HQE34360.1"
                     /translation="MKNKSILLKTSFFLVLILLFAYTKKESLVSGINLKNMDTKVNPG
                     DNFYDYVNGTWLKNNKIPSDKAYYGSSTLINDKAQEDVKAIIENASKGKFAYGSDEQK
                     IGDFYEAYMDTINREKIGIAPLLPEFKKINAISNYSDLAGYFGYANSIGFNTPLAIGI
                     TVDFKDPNQYMLYTWQSGLGLPDREYYILEDDKSKEIRKKYVTHIAKMLQLGGVENSS
                     ELAPKIMALETTLAGKHMKKEETRDMVKLYNKYEIKDLNTLMPDFNWSLMFKNAGIIN
                     QKNITVTQIEYIKSLSSIIKTTSLNTWKAYLKWSTIDNSAKYLNAALDIENFEFKGKT
                     LWGMKKQVPMWRR"
     gene            1367..1681
                     /locus_tag="PLQ70_06795"
     CDS             1367..1681
                     /locus_tag="PLQ70_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HQE34361.1"
                     /translation="MKIQFNTDKNIEGHERLEKFFSSELEKELARFDDKVTRIEVHLG
                     DENSDKGGVNDKRCMIEARPAKMQPIAVTAHADSIEKAFFQATEKIKKVLNTTFDKQA
                     VR"
     gene            1895..2638
                     /locus_tag="PLQ70_06800"
     CDS             1895..2638
                     /locus_tag="PLQ70_06800"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HQE34362.1"
                     /translation="MKKLADKAKVVRKTFKIIGIFFLSLFALMLVSAIGLRIYFEKNK
                     TEIVKKINTQINDNILGEAKIGDIGYKFLVGFPNFTVVLKDVELKDSLFAIHKRPVLK
                     AGEIEVRLNVLSLLKKEVNIHKIVINNATIDLFKDKNGVSNSTIFKPKKKEPKKESST
                     TTSIDEVVLKNVTFISENQKGNKLFYFQIESLKSKIEYTNEGWKTKVNLKTFAKSLAF
                     NVLRGSFVKDKSIEGKLAVDFSSEKISFR"
     gene            2767..4383
                     /locus_tag="PLQ70_06805"
     CDS             2767..4383
                     /locus_tag="PLQ70_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQE34363.1"
                     /translation="MANNISSKLNKFALKVPLAASCVIKGDMSVVGDPEIVVNAVIKD
                     DELKTSYGAVKNCSFNGKFTNNYKNGLGCNDANSAIIITNFKGEYKEIPLIIPSAIIN
                     NLEKPVATGKFNSEYDVVKLRNLINENFIKFSAGEAKVNLDFKVDIVDLRLNKPHFTG
                     AIAIKDASFYYRPKNVTFQKTNIDLQFTQEALLIKQIKYQRKQNIVFLEGRVDNFLNL
                     YYDAPEKMIVNWKIYSPYLDVKQIVNVLSYHDKSTPTKRKTQSHSQSSDQFQNVFSKS
                     QVNLDLAVDKMAFNKMLANNFKINIKMINGGLYVKNGLIQGRNGSSLRFDAQLIPKNG
                     LMHFKSNLKLNEGNISNFLASFNNFGIKSFGPNNIKGKLSLSASLLGVLNANNELNKK
                     SIIGNVNFQVKDGELINFQPIQKIGKIVFSNRDVSHIAFSDLYGITTIKGEQIKVNEL
                     KITSNVLNIDVKGDYSLSHSGTNLGVRIPLRNPKEDYKITDPKARETARYKGIVVSVL
                     VVDGKNGETKIKLGKIAEETPKETQKAKKK"
     gene            4474..5151
                     /gene="trmB"
                     /locus_tag="PLQ70_06810"
     CDS             4474..5151
                     /gene="trmB"
                     /locus_tag="PLQ70_06810"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQE34364.1"
                     /translation="MGSKNKLKRFKENETFNNVFQPSREEVVGNLFPLRGKWNADFFK
                     NDNPIVIELGCGKGEYSVGLAEKYPNKNFVGIDIKGARFWRGAKTAVETGLHNVAFIR
                     TQIELINHIFAENEVDEIWITFPDPQIKYKRTKHRMTNSDFLQLYKKILKKDGVVNLK
                     TDSEFMHGYTLGLLHGEGHEVLYANHNVYVNEGSPEEVTAFQTFYEKQYLEINKAITY
                     IRFKIKE"
     gene            5177..5809
                     /locus_tag="PLQ70_06815"
     CDS             5177..5809
                     /locus_tag="PLQ70_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HQE34365.1"
                     /translation="MNYITPLFFGFISAFIGIIPPGLINMTAAKVNHKEGKRNALWFV
                     FGAVLIICVQVFFSILFAKVINRRPDLVTLLREIGFGIFSTLTVYFLWLAKKPKDKTK
                     KYTQNSKANRFFQGMLLSSLNFFPIPYYVFVSITLASYQLFSFEKTTILIFVNGVLIG
                     SFLVFYLYISFFNKIENKRDFIMKNMNTIIGCITGLVSIITLINIIKYYW"
     gene            5811..6146
                     /locus_tag="PLQ70_06820"
     CDS             5811..6146
                     /locus_tag="PLQ70_06820"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HQE34366.1"
                     /translation="MGKSADNFFEKVYAIARQIPYGKVTSYGAIAKALGAARSARMVG
                     WAMNAAHNLEDVPAHRVVNRKGLLTGKHHFDGTNLMQQLLESEGIIVIDNQIVDLEKH
                     FWTPEMSLH"
     gene            6370..7494
                     /locus_tag="PLQ70_06825"
     CDS             6370..7494
                     /locus_tag="PLQ70_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HQE34367.1"
                     /translation="MKLDKKEILKALETITVAGEGKNMVESGAIANVVTFGDEVIVDL
                     VLHVPALHIRKRAEDDIKKTILEKISPEAKIIINVKVEAPEKSIAPDKSIPGIKNIVA
                     VASGKGGVGKSTVTANLAVTLAKMGFSVGILDADIYGPSMPIMFDVESAKPLSVEVDG
                     KSKMKPIENYDVKILSIGFFTPANQAIMWRGPMAAKALNQMIFDADWGELDFLLIDLP
                     PGTGDIHLSIVQALPLTGAVVVSTPQAVALADAKKGVAMFMSEAINVPVLGIIENMAY
                     FTPEELPNNKYYIFGKEGAKNLAEDLAIPFLGEAPIVQSIREAGDFGRPAALQTGSVI
                     ETVFENITRNVVQEVVNRNENLPATEVVKITTMAGCSSKK"
     gene            7534..7773
                     /locus_tag="PLQ70_06830"
     CDS             7534..7773
                     /locus_tag="PLQ70_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQE34368.1"
                     /translation="MTTEELTTNILKALEEIRPFLNSDGGDIQLVSVEDGKHVKVRLQ
                     GACTNCSVNHMTLRAGVETTIKKYAPQIETVVNVG"
     gene            7781..>8061
                     /locus_tag="PLQ70_06835"
     CDS             7781..>8061
                     /locus_tag="PLQ70_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019037658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQE34369.1"
                     /translation="MDITIKIKDRKGQIHEVKAPTDMALNLMHVVRAYELEPVGTIGL
                     CGGMALCHTCQCYTTNDVALPEKREAELATLSRLPNTKSNSRLSCQIPVT"
BASE COUNT         2849 a         1199 c         1413 g         2600 t
ORIGIN      
        1 ctctacgcca cattggcact tgttttttca ttccccaaag ggttttgcct ttgaattcaa
       61 agttttcaat atctaaagca gcgttcaaat actttgctga attgtcaatt gtactccatt
      121 ttaaataggc tttccaagtg tttaatgaag ttgtttttat gatagaactt agcgatttaa
      181 tgtattctat ttgtgtgaca gttatatttt tttgattaat aatccctgca ttcttaaaca
      241 ttaaactcca gttaaaatca ggcataagag tatttaaatc ctttatttca tacttgttgt
      301 aaagtttaac catatctcgt gtttcctctt ttttcatatg cttaccagcc aaagtggttt
      361 ctaatgccat aattttagga gcaagttcag acgaattttc tacgccaccc aattggagca
      421 tttttgcaat atgagttaca tattttttgc gtatttcttt tgatttgtcg tcttcaagaa
      481 tataatattc tctatcagga agtcccaaac cactttgcca agtataaagc atatattgat
      541 ttggatcttt aaaatctact gtaattccaa ttgcaagagg ggtattgaaa ccaatactat
      601 tggcataacc aaaatagcca gctaagtcag aatagtttga aatggcattt atctttttga
      661 attcaggcag taatggtgcg attccaattt tttctctatt aatggtatcc atataggctt
      721 cataaaaatc accaattttt tgttcatctg aaccataagc aaattttcct ttagatgcat
      781 tttcaattat ggcttttaca tcctcttgtg ctttatcatt aattagtgtt gaagatccat
      841 aataggcctt gtctgatggt attttgttgt tttttaacca agtgccattg acataatcat
      901 aaaaattatc tccagggttt accttagtgt ccatgttttt taggtttatt ccagatacta
      961 gagattcttt tttggtatag gcgaaaagaa gaattaaaac taaaaaaaat gaagttttga
     1021 gaagaatgga tttgtttttc atgagtttaa tttttttgta aattcaaata tagctaaaaa
     1081 tgaaagggaa tgtattgata gccagtaatt ttattgatgc aaatgtcaac ctcctagaaa
     1141 tttaattttg gatgaaatta tacgtatcat atgaatcaaa taagttttac tattttttaa
     1201 tttttatcct tttaatctgg caaactcttt ttctcttttc tctattgtta attgccttaa
     1261 ataaagaaaa attttgccct gtatttattt cataaagttt tattaattga actgttttta
     1321 taaaaataac tcgctatttt tgagacaatc aataaataat ttacttatga aaattcaatt
     1381 taatacagac aagaacattg aaggtcacga aagattagaa aaatttttca gttcagaatt
     1441 agaaaaagag ttagctcgtt ttgacgataa agtaactcga attgaggtgc atcttggaga
     1501 cgaaaatagc gacaaaggcg gagtgaacga taaacgttgt atgatagaag ctcgcccagc
     1561 caaaatgcaa cctattgctg taactgctca cgccgattcg atagaaaaag ccttttttca
     1621 ggcaacagaa aaaataaaga aagttttaaa tactactttt gacaaacaag cagtacgcta
     1681 agttttttta aaggaatttt aataaattcg tttttaagca gtctaaaaaa aagtttttta
     1741 gactgctttt tttatggatt attctgaatt cttttttggt atgctttatt ctagatagta
     1801 atgaactaag tgtttgattc ttagtgaaaa attcaaaaaa tattttattt aataaaacat
     1861 atattatctt tatttaaatt aaaggtattg gagtatgaaa aagttagccg ataaagccaa
     1921 agttgttaga aaaaccttca aaataattgg tatattcttt ctgagtttat ttgctttaat
     1981 gctggtaagt gcgattggct tacgtattta tttcgaaaaa aataaaaccg aaatagtaaa
     2041 aaaaatcaat actcaaatta atgataatat tctgggagaa gccaaaattg gtgacattgg
     2101 atacaaattc ttagtaggat tcccgaattt cactgtggtt ttgaaagatg tagagctgaa
     2161 agatagtttg tttgccattc acaaacgtcc tgttctaaaa gcgggtgaaa ttgaagtgcg
     2221 tttgaatgtt ttaagtttat tgaaaaaaga agtcaacatt cacaaaattg tcattaacaa
     2281 cgcaactatt gacttgttta aagacaaaaa cggagttagc aattctacta tttttaagcc
     2341 taaaaaaaaa gaacccaaaa aggaaagcag caccacaact tctattgatg aggtggtttt
     2401 gaaaaatgtt acttttattt ctgaaaacca aaaagggaac aaactatttt attttcaaat
     2461 agaatcttta aaaagtaaaa ttgagtacac taacgaaggt tggaaaacca aagtcaatct
     2521 gaaaaccttt gccaaaagtc ttgcatttaa tgttttgaga gggagttttg tgaaagacaa
     2581 gtcaatagaa ggtaaactgg cggttgattt ttcgagtgaa aaaatctcat ttcggtagtt
     2641 actgaagatt ttgaaatagg aagagatcct tttactatca aagctcattt taatatcggg
     2701 aaaggcaatt ctttatttga tattgatatt agaactaaga ttttgtagag taatgcctct
     2761 aatttattgg ccaataatat tagttcaaag cttaacaaat ttgctttaaa agttccactt
     2821 gcagcatctt gtgtcataaa aggtgatatg agtgttgtgg gtgatcccga aattgtggtt
     2881 aatgctgtta taaaagacga tgaacttaaa acctcatatg gcgcggtaaa aaattgtagt
     2941 tttaatggaa aattcactaa caattacaaa aatggtttag gatgtaatga tgcaaattca
     3001 gctataatta tcactaattt taagggcgaa tacaaagaaa ttccattaat aattccttca
     3061 gctattatca ataatcttga aaaacctgtg gcaactggga aattcaattc agaatatgac
     3121 gtagttaaat tgagaaactt gattaatgaa aattttatca aattttcagc aggagaagcc
     3181 aaagtaaacc tcgattttaa agttgatatt gtcgatttga ggttgaataa gcctcatttt
     3241 accggagcta ttgctatcaa agacgccagt ttttattata gacccaaaaa tgttactttt
     3301 caaaagacaa acatagactt acaatttacc caagaggctt tgttgataaa acaaatcaaa
     3361 taccaaagaa aacagaatat tgttttttta gaaggaagag tggataattt tctcaattta
     3421 tattatgatg cacccgaaaa aatgattgtt aattggaaaa tctacagccc ttatttagat
     3481 gttaaacaaa tagtgaatgt tttgtcctat cacgataaat caactcccac aaagaggaaa
     3541 acccaatccc attctcaatc ttccgatcaa tttcaaaatg tatttagtaa atcgcaagta
     3601 aatcttgatt tggcggtcga taaaatggcg ttcaacaaaa tgttggccaa taattttaag
     3661 attaatatca aaatgattaa cggcggatta tatgtcaaaa acggattgat tcaaggcaga
     3721 aacggaagct cgttaagatt tgatgcccaa ttgattccaa aaaatggact aatgcatttc
     3781 aaatccaatc taaaattaaa tgagggtaat atttctaatt ttttagcctc gttcaataat
     3841 tttggcataa aatcttttgg tccaaacaat ataaaaggga aactttcact ttctgcatca
     3901 cttttaggag tccttaatgc aaataatgag ttaaacaaaa aatctattat aggaaacgtc
     3961 aattttcaag ttaaagatgg agaattaatt aattttcaac ccattcaaaa aataggaaaa
     4021 attgtttttt ctaatagaga tgtaagccac attgctttta gcgatttata cggaatcacg
     4081 accataaaag gtgaacaaat aaaggttaat gagttgaaaa tcacatcgaa tgttttgaat
     4141 atagatgtaa aaggcgatta ttctttgtcg cattctggga ctaatctagg ggtgagaatt
     4201 ccgttgagaa atccaaaaga ggattataaa attaccgatc caaaagcaag agaaacggct
     4261 cgatacaaag gaattgttgt aagcgtactc gtagttgacg gtaaaaatgg tgaaactaaa
     4321 attaaattag gcaaaatagc tgaagaaact ccaaaagaaa cccaaaaggc taaaaagaaa
     4381 taggatttgg attccgtctt attatatttc cctaattttg ccagccaaaa cctttaggtc
     4441 gaattgtaag aagtaaaaat attttataaa aaagtgggaa gtaaaaataa attaaaaaga
     4501 ttcaaggaaa acgaaacatt caacaatgtt tttcaacctt caagagaaga agtagtaggc
     4561 aatttgtttc cgcttagagg caaatggaat gccgattttt tcaaaaacga caatccaata
     4621 gttattgaat taggttgcgg aaaaggagaa tattctgttg gacttgctga aaaatatccc
     4681 aataaaaact ttgttggcat cgatatcaaa ggagcgcgtt tttggcgagg tgccaagact
     4741 gcagtagaaa ccggtttgca taatgtagct ttcattcgta cccaaatcga attaatcaac
     4801 catatttttg ccgaaaatga agtcgatgaa atttggatta cttttcccga tcctcaaatc
     4861 aaatacaagc gtaccaaaca tagaatgacc aattcagatt ttctgcaatt gtataaaaaa
     4921 atcctcaaaa aagatggagt tgtcaacctg aaaaccgata gcgaatttat gcacggatat
     4981 actcttgggt tgctgcacgg cgaaggtcac gaggtgttgt atgccaatca taatgtgtat
     5041 gtaaatgaag gaagtcccga agaagtgact gcttttcaga ctttttacga aaagcaatat
     5101 ttagaaatca acaaagccat tacttatatt cgatttaaaa ttaaagaata atcttttcaa
     5161 ctccgacaaa tcttaaatga attatataac gcccctattt tttggtttta ttagtgcttt
     5221 tataggaatt attccacccg gattaatcaa tatgacagcg gcaaaagtaa accataaaga
     5281 aggaaaacga aatgcgttat ggtttgtgtt tggagcagtt ttaatcattt gtgttcaagt
     5341 ttttttctcc attttatttg caaaagttat taatcgacga ccagatttgg tgactttatt
     5401 gcgcgaaatt ggattcggaa tattttcaac tttgacggtc tattttttat ggcttgccaa
     5461 gaaaccaaaa gacaaaacca aaaaatacac tcaaaacagt aaagccaatc gtttttttca
     5521 gggaatgttg ctttcctctc tcaacttttt cccaattcct tattatgttt ttgtgagtat
     5581 tacattagca tcctatcaac tgttttcatt tgaaaaaacg acaattttaa tatttgtaaa
     5641 cggcgtttta ataggctctt ttctggtctt ttatttgtat atttcttttt ttaacaaaat
     5701 tgaaaataaa agagatttca taatgaaaaa tatgaataca attattggtt gtatcacagg
     5761 attggtttca ataattactt tgattaatat cataaaatat tattggtaaa atgggtaagt
     5821 cagccgataa ttttttcgaa aaagtgtatg ctattgcaag gcaaatacct tacggcaaag
     5881 tcacttctta tggtgccatc gcaaaagcat tgggcgcagc tcggtcagcc agaatggtgg
     5941 gttgggcaat gaatgcagca cataacctag aagatgttcc agcgcatcgt gtggtaaata
     6001 gaaaaggact tctgactgga aaacaccatt ttgatggaac aaatctaatg caacaattgc
     6061 tcgaaagtga aggaattatt gttatagata atcaaattgt cgatttagaa aaacattttt
     6121 ggacgccaga aatgagtttg cattaattat ttttaaaaat tattataaca ttcatttact
     6181 gccgaagcaa tttctgctta attaaaaatt aaaactcatt attgtttctt tatttccaaa
     6241 attagaactc cttaaacatt gtcactatac aacttcttca ctatatttgt agtcaagaat
     6301 ttaatagatt cagtttaaat aaatataata tttcagttga gtaatttcag ttgaaaacac
     6361 aatcagaata tgaaattaga taaaaaagaa atccttaaag cattagaaac catcacggtt
     6421 gcaggcgaag gaaaaaatat ggtcgaaagc ggtgccattg caaacgtagt gacctttgga
     6481 gatgaagtaa ttgttgattt agtattgcac gttccagcgt tacatattag aaaacgtgcc
     6541 gaagacgata tcaaaaaaac tattttggag aaaatttcac cagaggctaa aataattatt
     6601 aatgtcaaag ttgaggctcc agaaaaaagt atcgctccag acaaatcaat acctggaatc
     6661 aaaaatatag tagctgttgc ttcaggaaaa ggtggggtag gaaaatctac tgttaccgca
     6721 aacttggctg taacgctagc aaaaatggga ttctcagtag ggattctcga tgccgatatt
     6781 tacggcccat ccatgccaat aatgtttgat gtagagagtg caaaaccact ttcggtcgaa
     6841 gtggatggta aatcaaaaat gaaacctatc gaaaattatg atgtcaaaat actttcgata
     6901 ggatttttta ctcctgcaaa tcaagcgata atgtggcgtg ggccaatggc tgcaaaagct
     6961 ttaaatcaaa tgatttttga tgctgattgg ggcgaattag actttttatt gattgatttg
     7021 ccaccaggaa caggagatat tcatttgtcc atagttcaag ctttaccact tacaggtgca
     7081 gttgtcgtga gtacaccaca agccgtggct cttgccgatg caaaaaaagg ggttgccatg
     7141 tttatgtcag aagctataaa tgttcctgtt ttgggaatta tagaaaatat ggcttatttt
     7201 acaccagaag aattgcctaa taataaatat tatatttttg gaaaagaggg agcgaaaaat
     7261 cttgctgagg atttagcaat accatttttg ggagaagctc caatagtaca atctattcgt
     7321 gaagcaggag attttggtcg tccggcagcc ttgcaaacag ggtcagttat agaaactgtt
     7381 tttgagaata tcactagaaa tgtagttcaa gaagtagtga acagaaacga aaacttgcct
     7441 gctaccgaag tggtgaaaat aacaacaatg gcaggttgtt catccaaaaa ataatagcaa
     7501 aactcgttta gaagggaaat taatttaaat aaaatgacta cagaagaatt aacaacaaac
     7561 attttgaaag cgctcgaaga aattcgccct tttttaaatt ctgatggagg agatatacaa
     7621 cttgtttctg ttgaagatgg aaaacatgtg aaagttcgtc ttcaaggtgc ttgcactaat
     7681 tgcagtgtaa atcacatgac gcttagagca ggcgtagaga caactatcaa aaaatatgcg
     7741 cctcaaatcg aaacggttgt taatgttgga taattgattt atggatataa caataaagat
     7801 taaagatcga aaaggtcaaa tacacgaagt taaagcccca acagatatgg cattgaactt
     7861 aatgcatgtt gttcgtgcct atgagcttga acctgttgga acaattggtt tgtgtggcgg
     7921 aatggccctg tgtcacacct gtcaatgtta cacaacaaat gatgtcgctt tgcctgaaaa
     7981 aagagaagct gaattggcga ccctttcgag attgccaaat acaaaatcta acagtcgttt
     8041 gagttgtcaa atcccagtta c
//
LOCUS       DAOSNN010000109         7965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9062_length_7965_cov_4.612137, whole genome shotgun sequence.
ACCESSION   DAOSNN010000109 DAOSNN010000000
VERSION     DAOSNN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9062_length_7965_cov_4.612137"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..745
                     /locus_tag="PLQ70_06840"
     CDS             341..745
                     /locus_tag="PLQ70_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQE34370.1"
                     /translation="MKSKYLLLVVFAILFFVSNTVIAQKKWAVPEYTKGLKNPFDGNA
                     VATNEGKVIYGQMCVLCHGISGKGNGEAGLSLEKKPANFLALKNIPNETDGEIFWKIT
                     VGKPPMSSYDELLTEDQRWKLVNYIRELNKKK"
     gene            complement(815..1828)
                     /locus_tag="PLQ70_06845"
     CDS             complement(815..1828)
                     /locus_tag="PLQ70_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQE34371.1"
                     /translation="MAQIANVSVGTVDRIIHNRGQVTEENIEKVNAIIKEYGYKKNIF
                     ASNLAFNKKFKFAVFLPKNENIEYWQAPIVGIKKAAEEFANFGVTIDYFFFEYNTTSF
                     NQIAQEILKLEYNGLLFAPIFYKESVSFLNEYKKKNIPIVMIDSNIEEIENIAYIGQD
                     SYQSGYLGGKLISYGIKENSNVLILEINRNAESTTRTNVYLQRIKGFYSYFKEKTNLP
                     KFNFSEISIQYDSKNQLNSTMFEGIDCVFIPNSRAHIVAKFIKDNHLKNIHLVGYDLL
                     KDNIEFLKDDTIAFLINQKPEDQGYKGISHLYKKVVLKEQVNEKQYLPLEIIVQENYT
                     KTI"
     gene            2012..2824
                     /locus_tag="PLQ70_06850"
     CDS             2012..2824
                     /locus_tag="PLQ70_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE34372.1"
                     /translation="MNSIFNLKGKVALITGGGGVLGGNMAEVLAKQGVITGIVGLTLE
                     EAEATVARVKNNGGEAFAVKANVLNKEELEVARDFVIEKYGKLDILINAAGGNMPGAT
                     IGPDQAIYDMQLAHLQKVIDLNIIGTMLPSQVFSEIFAKQKSGIIINLSSASAQRPLS
                     RVVGYSASKAAIDNFTKWMAVELAGKYGEGLRVNAIAPGFFIGEQNRALLLNPEGGLT
                     PRGEKIIDHTPMGRFGVPEDLDGVLLFLCSDMSKFVTGVVVHVDGGFGATSI"
     gene            2848..4008
                     /gene="uxuA"
                     /locus_tag="PLQ70_06855"
     CDS             2848..4008
                     /gene="uxuA"
                     /locus_tag="PLQ70_06855"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HQE34373.1"
                     /translation="MEQTWRWYGPNDPVKLSDAKQAGATGIVTALHHIKNGQVWEVEE
                     IMKRKNEVEAAGLTFSVVESIPVHEDIKKQSGDYLKYIENYKQSIKNLASCGIHIVCY
                     NFMPVLDWSRTDLSYEMPDGSKALRFDINEFAAFELFILKRPGAENTYTDAQKAKAKA
                     TFEKLTDADKIKLQQNIIAGLPGAEESYTVEDFLKVLQGYDGIDADKLKENLYYFLRE
                     IVPVAESVGVKMAIHPDDPPYPILGLPRVVSTEADLVQLLNAINSPSNGFCMCTGSYG
                     VIAKNDLSGMIDRNGEKMNFIHLRSTQRDEEGNFYEANHLEGNVDMYEVVKSIIKMEK
                     QTGRIIPMRPDHGHQMLDDLNKKTNPGYSGIGRLRGLAELRGLEMGIKRSLF"
     gene            4207..4674
                     /locus_tag="PLQ70_06860"
     CDS             4207..4674
                     /locus_tag="PLQ70_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34374.1"
                     /translation="MKNLSLVGLLLVCVGFGSCNKNQKAADAQALNEKPIEVKCYKAI
                     YEKDTINLKINTLNNNKVSGDMVMKFADMPVKDGKIAGEMRGDTLFADYTFALGANTF
                     KNPIAILKRGEELILGNGQIETYLGASYFAKGKPIDFDKVKFKFTAVDCVEKK"
     gene            complement(4693..5595)
                     /locus_tag="PLQ70_06865"
     CDS             complement(4693..5595)
                     /locus_tag="PLQ70_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQE34375.1"
                     /translation="MLNKDLNNSVNYPISSEEDELWGLTITTVGRQNICEKENYPPEK
                     HPVGYFFDVEKGRVLNEYQLLYITNGNGVFTYGNSKQSCFITEGKMFFLMPGVWHTYK
                     PLENSGWNEYWIGFKGEIIEKIVKEGFFLNKTPVFNLGMNEEIIDLYYKAIEIANEER
                     SGFQQALSGIVMNILGLMYYRNKTRAFEDQELINKINKAKVMMREDVYKNITAEDISK
                     NLGISYSGFRRAFKELTGTSPSKYMLELKLNEAKLLLNNTNLPIKEISYSLNFENPDY
                     FPIFFKKRTGKTPSEYRNFASNGS"
     gene            complement(5732..6994)
                     /locus_tag="PLQ70_06870"
     CDS             complement(5732..6994)
                     /locus_tag="PLQ70_06870"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009410459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HQE34376.1"
                     /translation="MKTEQINQAFEYAKSRYASLGIDVEKAVAEMDKLSISLHCWQAD
                     DVTGFESGNDELTGGIQVTGNYPGKARTIDELRADIDKAMSLIPGEHRVNLHALYGDF
                     GGEKVDRDQIEIKHFQSWIDWAKEKGYKLDFNATCFSHEKSSEGFTLSHPDPAIRQFW
                     IEHVIRCRKIAEEMGRQLGSKCIHNIWIPDGSKDLVIDRSQYRQNLKDSLDKIFEYKT
                     NDELMLDSIECKLFGIGSESFVVGSHEFYMGYGIANNKMITLDAGHFHPTEVISDKIS
                     SLLLFTPEILLHVSRGVRWDSDHVVILNDELQAIAQEITRANALERVHVGLDFFDASI
                     NRIGAYVIGVRATQKAFLQALLEPTLQLRSYEANGQNFERLALLEEAKSLPWAAVYDY
                     YCASKGIIAAEDYISDIQQYEKEVTSKR"
     gene            complement(7023..>7965)
                     /locus_tag="PLQ70_06875"
     CDS             complement(7023..>7965)
                     /locus_tag="PLQ70_06875"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQE34377.1"
                     /translation="TVAQVKEGVLEFSTSGADAIIAIGGGSPIDTAKGIGIISNNPEF
                     ADVVSLEGVADTKNKSVPIIALPTTAGTAAEVTINYVITDEENVKKMVCVDPNVIPVL
                     SIVDAELMLSLPPSLTAATGMDALTHAIEGYITKGAWEMSDMFELKAIEMIAKHLPVV
                     VKNPSDVVARDGMAVAQYIAGMGFSNVGLGLVHGMAHPLGAYYDIPHGVANALLLPIV
                     MEYNTESSIAKYVDIARAMGIKVDHLSLSEAAQAAVEAVKNLAIEVGIPEKLQLLNVK
                     EEDLERLSQSAFEDVCTPGNPREVQLNDILTLYKKAF"
BASE COUNT         2552 a         1269 c         1432 g         2712 t
ORIGIN      
        1 tatttttgat gcgcttcgtt ctttctcaat cagaactttt gtggcatcca aagacaatga
       61 atataacaat gctattttgc aagaattgtt aaatagcaaa gaatcaaact tcatcaatac
      121 agattatatt ttatagtcta aatttgatca atattcaaaa ccgcatcagt tttacttttg
      181 cggttttttt ggctcaaata ataactaaag aatttacaat cagtatcaca ttgctatatt
      241 tgttataata ttttaattat ttgaatattt tgtgatattt atgatgtttt attgaataat
      301 atgtagtaac tttattatgt aaattttaat ctatactatt atgaaaagta aatatttgtt
      361 attggtagtg tttgcaatac tgttttttgt tagtaataca gttatagctc aaaaaaaatg
      421 ggcagttcca gaatatacta agggattaaa aaatcctttt gatggtaatg cggttgcaac
      481 caatgaggga aaagtaatat atggacaaat gtgtgtgtta tgccacggta tttctggcaa
      541 aggaaatgga gaagctgggt tgagtttaga aaaaaaacca gccaatttct tggccttaaa
      601 aaacattccc aatgaaactg atggtgaaat tttttggaaa ataactgttg gaaaaccacc
      661 aatgtcttct tatgatgagt tgttaaccga agaccaacgt tggaaattag ttaattatat
      721 aagagaactc aataagaaaa aataattttt attccatttc aaatacaaag aagccatttc
      781 agtaaggaga cggcttcttt gtttgatttt tatcctaaat cgtttttgta tagttttctt
      841 gtacaataat ttctaaaggc aaatattgct tctcatttac ttgctctttt aaaactactt
      901 ttttatacaa atgactgatg cctttgtagc cttgatcttc gggtttctga ttgattaaaa
      961 aggctatggt gtcatctttt aaaaattcaa tgttatcttt caataaatca taacctacta
     1021 aatggatatt ttttaaatga ttgtctttga tgaatttagc aacaatgtgt gctctggaat
     1081 ttggaataaa aacacaatca ataccttcga acattgtact atttaattga tttttactgt
     1141 catattgtat gctaatttca gagaaattaa attttggaag atttgttttt tccttgaaat
     1201 aggagtaaaa ccctttaatt ctttgtaagt aaacatttgt tcttgtggtg ctttctgcat
     1261 ttcgattgat ttctaaaatc aatacattac tattttcttt tattccataa ctaatgagtt
     1321 tgcccccaag ataaccactc tgataggaat cttgaccaat gtaggcaatg ttttcgattt
     1381 cttcaatatt agaatcgatc atcacaattg gaatgttttt tttcttgtat tcgttcagaa
     1441 aagaaaccga ttctttataa aatatgggtg caaacagcaa tccgttgtac tctaatttta
     1501 ggatttcttg tgctatttga ttaaaagaag tagtgttgta ttcaaaaaag aagtaatcaa
     1561 tagtcactcc aaaattcgca aactcttctg ccgctttttt gattccaaca attggagctt
     1621 gccaatattc aatgttttca tttttgggga gaaagacggc aaatttaaat ttcttattga
     1681 aggctaaatt actagcaaaa atatttttct tataaccata ttctttgatt atggcattta
     1741 ctttttcaat gttttcttcc gttacttgac ctctattgtg gataatacga tcaactgttc
     1801 caactgaaac attggcaatt tgagctattt tttttattgt tataatgatt tcaagttttt
     1861 gtattgggct gtaaatttat cattttttgc ctcaatatat ttataattta tttttagttt
     1921 gacttaagtt tttttatata tttgcataag ccgtgtgcgt acacgaatat ataactattt
     1981 tttaaaacaa taataaatta atacacaaaa aatgaattca atttttaatt taaaaggaaa
     2041 agttgccctt ataacaggtg gtggcggtgt tttaggaggt aatatggccg aagttttagc
     2101 aaaacaaggc gtaattacag gaattgtggg acttacatta gaagaagcgg aagctactgt
     2161 tgcaagggtt aaaaataatg gtggcgaagc atttgcagta aaggctaacg tattaaacaa
     2221 agaagagcta gaagtggcta gagattttgt aatagagaaa tacggaaagt tagatattct
     2281 gattaatgca gcaggaggaa atatgcccgg agctactatt gggccagatc aggctattta
     2341 tgatatgcaa ttagcacatt tgcaaaaagt gatcgacttg aatatcattg gaaccatgtt
     2401 gccttcacaa gtgttttctg aaatttttgc taaacaaaaa tctggaatta tcatcaattt
     2461 atcatcggct tcggctcaaa gacctttgtc tagagtagtt ggttattcgg cttcaaaagc
     2521 agcaatcgac aattttacca aatggatggc agtagaatta gcaggtaaat atggcgaagg
     2581 tttgcgtgta aacgcgattg ctccaggatt ttttattgga gaacaaaacc gagctttgtt
     2641 gctaaatcct gaaggaggat taacaccaag aggtgaaaaa attatcgatc atactccaat
     2701 gggaagattt ggtgtgccgg aagatttaga tggtgtattg ttgtttttat gtagcgatat
     2761 gtcaaaattt gtaacgggtg ttgtggttca tgttgatggt ggttttggag ctacaagtat
     2821 ttaattataa ttaaaaatat ataaattatg gaacaaacat ggagatggta cggaccaaat
     2881 gatccagtaa aattatcgga tgccaaacaa gctggagcaa ctggaattgt gactgcgcta
     2941 catcatatca aaaatggtca agtgtgggaa gttgaagaaa ttatgaagcg taaaaatgaa
     3001 gtggaagcgg ctggattaac cttttcagta gtcgaaagta ttccagttca tgaggatatt
     3061 aagaaacaat caggcgatta tttgaaatat attgaaaact acaagcaaag tatcaaaaac
     3121 ttggctagtt gtggaattca cattgtttgt tataatttta tgcctgtttt agattggtct
     3181 agaactgatt tatcatacga aatgcctgat ggttcaaaag ccttgcgttt tgatattaat
     3241 gagtttgcag cctttgaatt gtttattttg aaacgtccgg gagccgaaaa cacttatact
     3301 gatgctcaga aagccaaagc caaagctact ttcgaaaaat tgactgatgc tgataaaatt
     3361 aaattgcaac aaaatattat tgctggtttg ccaggagcag aagaatcata tacagttgaa
     3421 gatttcttaa aagtacttca aggatatgat ggaattgacg ccgataaatt aaaagaaaat
     3481 ttatattatt tcttgcgtga aatcgtgcct gtggccgaga gtgttggtgt aaaaatggcc
     3541 attcatcctg atgatcctcc ttatcccatt ttaggattgc caagagtagt aagcaccgaa
     3601 gccgatttag tacaacttct caatgccatt aactctcctt ctaatggatt ttgtatgtgc
     3661 accggttctt atggagtaat tgcgaagaat gatttgtcag gaatgattga tcgaaatggt
     3721 gagaaaatga attttattca tttgagaagc actcaacgtg acgaagaagg aaacttctat
     3781 gaagccaatc atcttgaagg gaatgtcgat atgtatgaag tagtgaaatc tattattaaa
     3841 atggaaaaac aaactggtag aatcattccg atgcgtcccg atcatggtca tcaaatgctg
     3901 gacgatttga acaaaaaaac caatccaggt tattcaggaa ttggccgttt gagaggactt
     3961 gctgagttgc gtggattaga aatgggaatt aaaagaagtt tgttttaata gcaaatttca
     4021 gtttctataa tttaaaaacc ttaaaccaat cgactgtgat ggtttaaggt tttttttagg
     4081 gaacaaaatt tctttttaaa tttagaaagc agttataatt accgatatat aagtataatt
     4141 atttgctttt ttttcttatt ttagcattgg ctattgtcgc attattaaat taaaaaatgg
     4201 attattatga aaaatctaag tttggttggc ttactattag tttgtgttgg attcggaagt
     4261 tgtaataaaa atcagaaagc tgctgatgct caagctttga atgaaaaacc aattgaggta
     4321 aaatgttata aagccattta tgaaaaagac accattaatt tgaaaattaa tactttaaat
     4381 aataataaag tttcaggaga tatggtgatg aaatttgcag atatgccagt taaagatggt
     4441 aaaattgctg gagaaatgcg aggagatacc ttgtttgccg attatacttt tgctttaggt
     4501 gcgaatactt tcaaaaaccc aattgctatt ttaaaaaggg gagaggaact gatattgggt
     4561 aacggtcaaa tagaaactta tttaggcgca tcttactttg caaaaggcaa accaatagac
     4621 tttgataaag tcaaatttaa gtttactgct gtggattgcg tcgaaaaaaa ataagtcgat
     4681 tttttaaata atctaactcc cattggaagc aaaatttctg tattcgctgg gagtttttcc
     4741 tgttcttttt ttgaagaaaa tagggaaata atcagggttt tcaaagttta agctatacga
     4801 tatttctttg ataggcaaat tggtattgtt caataaaagt ttggcttcat tgagttttaa
     4861 ttccaacata tattttgaag gagaagttcc tgttagctct ttaaaagctc tacgaaaacc
     4921 agaatagcta atacccaaat ttttggaaat gtcctcggcg gtgatgtttt tgtaaacatc
     4981 ttcgcgcatc attactttgg ctttgttgat tttattgatg agttcttgat cttcaaaagc
     5041 tcttgttttg ttgcgataat acatcaagcc caaaatattc atcacaattc cagaaagtgc
     5101 ttgttgaaat ccagaacgtt cctcgttggc aatttctatc gctttgtaat acaaatcgat
     5161 gatttcttca ttcataccaa gattaaatac gggagtttta ttcaagaaaa agccttcttt
     5221 tacaattttt tcgatgattt cgcccttaaa accaatccaa tattcattcc aacccgaatt
     5281 ttctaatggt ttgtatgtgt gccaaactcc aggcattaaa aaaaacattt ttccttcggt
     5341 aatgaaacaa gattgcttcg aattgccata agtaaagact ccattgccgt ttgtgatata
     5401 caacagttga tattcattta gaacacggcc tttttctaca tcgaaaaaat aaccaacagg
     5461 atgtttttcg ggaggatagt tttccttttc acaaatattt tggcgtccaa ctgtggtaat
     5521 ggtcaaaccc cataactcat cttcctcaga agatatgggg taattaacac tattattgag
     5581 gtctttatta agcatcaaat aaaaaattca ggatgaaatt ttatggtagt tttcattaca
     5641 aatataacga ataataccgt tttaacttta gaaataaaat caaaaaaact ccccaatctc
     5701 tttatttatt tgaaattggg gagcagtata attatctttt tgaagttacc tctttttcat
     5761 attgttgaat gtctgaaata taatcttcgg cagcaataat tccttttgat gcacaatagt
     5821 aatcataaac agcagcccaa ggcaaggatt tagcttcttc tagcaaagcc aaacgctcaa
     5881 aattttgacc atttgcttca taagaacgca attgaagggt tggctccaat aaggcttgca
     5941 agaatgcttt ttgagtggca cgaactccaa taacataagc gccaattctg ttgatggaag
     6001 catcaaagaa atctaaacct acgtgaactc tttctagggc attggcacga gtaatttctt
     6061 gtgcaatggc ttgcaattcg tcgtttagaa tcacaacgtg atcgctatcc caacgaacac
     6121 cacgacttac gtgaagcaag atttcaggtg taaacaacaa taaggaagat attttatctg
     6181 aaatgacttc ggtaggatga aaatggccag catcgagtgt aatcattttg ttattagcaa
     6241 tgccataacc catataaaat tcgtgtgagc ctactacaaa actttcgctt ccaataccga
     6301 ataatttaca ctcgatacta tctagcatta gttcgtcatt ggttttgtat tcgaaaattt
     6361 tgtctaatga atcttttaga ttttgacggt attgtgaacg gtcaatcact aaatctttgg
     6421 aaccatcagg aatccaaatg ttgtgaatac attttgaacc caattgacgt cccatttctt
     6481 cagcaatttt gcgacaacga atgacgtgtt caatccaaaa ttgacggata gctggatctg
     6541 gatgtgataa tgtaaaacct tctgatgatt tttcgtgaga gaaacaagtg gcgttgaaat
     6601 ctaatttgta tcctttttct tttgcccaat caatccaact ctgaaaatgt ttaatttcga
     6661 tttggtcacg atctactttt tctcctccaa aatcaccata caatgcgtga agatttactc
     6721 ggtgttcgcc aggaattaat gacattgctt tgtcgatatc agcacgaagt tcgtcaatgg
     6781 ttctggcttt tccaggataa tttcctgtaa cttgaatgcc tcctgttaat tcgtcattgc
     6841 ccgattcaaa accagttaca tcgtcggctt gccaacagtg aagcgagata gataatttat
     6901 ccatttcggc aactgctttt tcaacatcaa ttccaagact agcatatctt gatttggcat
     6961 attcaaatgc ttgattaatt tgttctgttt tcattatttt taattttttt atttgtggta
     7021 agttaaaatg cttttttata aagtgttaaa atgtcgttta attgtacttc acgaggattt
     7081 cctggcgtgc aaacatcttc aaaagcagat tgagacaaac gctccaaatc ttcttctttt
     7141 acattcaaca attgtaattt ttctggaata cctacttcaa tagccaagtt tttcacagct
     7201 tcaactgccg cttgagccgc ttctgaaagt gataaatgat ctacttttat tcccatagca
     7261 cgcgcaatat ctacatattt ggcaatggac gattcggtgt tgtattccat aacaattggc
     7321 aataataaag cattggcaac accgtgtgga atgtcataat aggctccaag tggatgtgcc
     7381 attccgtgta ccaaacctaa tccaacattc gaaaatccca ttcctgcgat gtattgagca
     7441 accgccattc cgtcgcgagc tactacatct gaaggatttt tgacaactac tggcaaatgt
     7501 ttagcaatca tttcgatagc tttcaattca aacatatccg acatttccca agctcctttg
     7561 gtaatatatc cttcgatggc gtgggtcaag gcatccattc ctgttgcagc ggtcaaactt
     7621 ggtggcaaac tcaacattaa ttcggcatct acaatagaaa gaactggaat aacatttggg
     7681 tcaacgcaaa ccattttctt tacattttct tcgtcggtaa tcacgtaatt gatggttact
     7741 tcggcagccg ttccagcagt ggttggcaaa gcgataatcg gaacggattt atttttggta
     7801 tcggcaacac cttctagtga aaccacatcg gcaaattcgg gattgttgga tataattcca
     7861 attcctttgg cagtatctat tggtgaacct ccaccgattg ctataatcgc atcagcacca
     7921 gatgtactaa attctagcac gccttctttt acttgggcaa ctgtt
//
LOCUS       DAOSNN010000110         7858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9217_length_7858_cov_5.277586, whole genome shotgun sequence.
ACCESSION   DAOSNN010000110 DAOSNN010000000
VERSION     DAOSNN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9217_length_7858_cov_5.277586"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..2345
                     /locus_tag="PLQ70_06880"
     CDS             108..2345
                     /locus_tag="PLQ70_06880"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34378.1"
                     /translation="MKKTILFCILLVATKAFTQTIPANEVSLDQMLVNIDKTTVTSGI
                     IYERIAPFTNLYNFNTKTNRNIADFILFKQALLEMYLASNQLQFSSVETLNNNLNPIL
                     YDDASVYMGVLNTPFQSVNYNEENPSLGRLQYDETNFLFYASTNTSYPFFNSSFATVI
                     SPLKDVVKGDYISFNWASSFIFENTLNSTKKIKTLTVNYGTGIDYTVISNYVIASATQ
                     QITYPDTGEKQLTFTVTYSDNSSLTTYAKIKYLKVVNDPLHRIMGSDPNCGTDYGLTE
                     NRTSTSEYAFQGYDEPYAFKGFINYKVFYKTNGTQKITKPIIIIDGFDPGDKRQVLPC
                     DYEPFEYKVGESRAISDVMKYGVNKENDLIKELQYKGYDVVIVNQPTYYIMTTPPYQI
                     VPRGTAGSREIDGGGDYIERNGLNLVSLIQKINTELTTNGSTEQLVIVGPSMGGQISR
                     YALAYMEKNNIPHNTRLWVSVDSPHLGANIPLGAQAEINLLKSSNDQAKDFYDKQLGS
                     VAARQQLIEFHKEIVTYVTFGTLTVPLLRTVDQSLLNARTISQGFSTNSGSSFFQNFY
                     NNQFSNGLPNSKGYPMNLRKIALVNGSLTGSKTGLDSQQTMNIRGFADICLPFCFKVH
                     AASMEVYTMPIFGNTSTIARFKSAFTDKNTFAPNLNSRGNMDIIPGGYFDSYNQLNNS
                     IMGQSGPTTNFAIPFHIDLSAKDINFESRTNTKIHSFIPTFSALGMKNPDQDLGSKFK
                     QKFSL"
     gene            2428..3291
                     /locus_tag="PLQ70_06885"
     CDS             2428..3291
                     /locus_tag="PLQ70_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE34379.1"
                     /translation="MFKELGDNINPPIAQAPTFPLSNSKLSGIDKICVGQTSTYSFTD
                     ICKIPSAVTWSVSSNLQIMSFTATSIIIKGLTSDQGVVTATFQNGMSVSKNVWVGVPA
                     FNLLRTSIETCDGIKYHYVNFEINPPPGSGTTFKFFYPNANVTYTMTSYGKYQFRFVE
                     TFDDFFDIAVTATNSCGTYSIYSEEYISSCTGGALSRLNAPTKTKKYKIYPNPTSDII
                     NISLVDEAQKPVTTSKIVAELYDFTGQPKYKVEVKNNIASINCNGLSSGIYILKIIID
                     GNVESHQVFVQ"
     gene            3399..4313
                     /locus_tag="PLQ70_06890"
     CDS             3399..4313
                     /locus_tag="PLQ70_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6268 family outer membrane beta-barrel
                     protein"
                     /protein_id="HQE34380.1"
                     /translation="MIKKLIFISLLFLSQFSNAQKYVDLFSINYGKSAVTSYENGSQS
                     TTITNIDIDFTLPIKLDEKNAIITGIDFSNNILQLFPDASYNNLYLTRIKTGINIIHS
                     EHWSGTYIFLPKISSDYINVSMEDFYIGGLVVLKYRKRNNLSYKFGLYAGNEAFGLFI
                     SPVIGLYYLSPNSSFEMNLYLPSNADINYSLTNKTKAGIDFVARGKSYKLTTDDVRSS
                     YAENNSLEFSSYLQNNSLNKNMLIRLKLGFSTNSFEVYPIDQKITFATTLLKFGDHRT
                     QLNSTLSSSPFIKIEAIYRFDIPTSKEK"
     gene            4438..5499
                     /locus_tag="PLQ70_06895"
     CDS             4438..5499
                     /locus_tag="PLQ70_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HQE34381.1"
                     /translation="MNKNYYAIIMAGGVGSRFWPVSTAEFPKQFHDMLGAGETLIQKT
                     FSRLSKLIPVENILVLTNEKYNAIVLEQLPLVKQEQVLLEPAMRNTAPCILYASLKIQ
                     KQNPDAVMVVAPSDHWIEDENEFAANLKQCFDFCANENALMTLGIQPTFPNTGFGYIE
                     YDKTDANPIKKVSQFREKPDYETAKTFLQSGNFLWNGGIFIWSAKSITEAFEKFQPQM
                     NALFLKGLESYNTPAEKTFIEENYALSENVSIDYAVMESAKNVYVLPATFDWNDLGTW
                     GQLHEKLDKDENKNGVINAKVVLENASGNIIRSDADKIIVVDGLHDYIIVDKEGVLLI
                     YPKSKEQDIKRITTLANKL"
     gene            complement(5598..6359)
                     /locus_tag="PLQ70_06900"
     CDS             complement(5598..6359)
                     /locus_tag="PLQ70_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQE34382.1"
                     /translation="MIEIKNIEKSFGETKILKGVSTIFEAGKTNLIIGQSGSGKTVLL
                     KSLLGIHTPEVGTISFDGRIYSDLNSDEKRELRTEIGMLFQGSALFDSMTVSENVGFP
                     LKMFTNDNKKKIKERVDFVLERVALVGAHNKLPSEISGGMQKRVAIARAIVNNPKYLF
                     CDEPNSGLDPNTATLIDNLIREITEEYNITTVINTHDMNSVMEIGKKIVFLKNGLKEW
                     EGTKEEIFRTDNEAVVDFVYSSNLFKKVREAYLKE"
     gene            complement(6359..7105)
                     /locus_tag="PLQ70_06905"
     CDS             complement(6359..7105)
                     /locus_tag="PLQ70_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE34383.1"
                     /translation="MMLLRYLSQIGKYFLMWKEIFSKQTKWSVMRGLIFKEIDDLIID
                     SLGIVAFISFFVGGVVSIQTALNLNNPLIPKTLIGYATRESVILEFAPTFISVIMAGK
                     MGSFITSSIGSMRVTEQIDALEVMGVNSLNYLVFPKLIAVLLYPFVIGISMFLGVFGG
                     YIAAVYGGFGASSDFIDGLQINFEPFHVTYAFIKTFIFGILLASIPAFHGYYMKGGAL
                     EVGKASTVAFVWTSVLIILVNYILTQLLLT"
     gene            complement(7173..>7858)
                     /locus_tag="PLQ70_06910"
     CDS             complement(7173..>7858)
                     /locus_tag="PLQ70_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQE34384.1"
                     /translation="PWIQLVNKTSENIHLPGSKVIQAFQKGFETLDENYDLIVKIDAD
                     LIFPTNYFETIIKHFQSDERIGMVGGFCYIEKNGNWILENLTDKDHIRGALKAYRTAT
                     FKQIGGLKPAMGWDTVDELLCKYYNWKVVTDESLHVKHLKPTGANYNKTARYKQGEAF
                     YTLGYGFWITSIASAKLAMMKKKPLLFFDYIQGFWKAKSAKKPMLVNPEQAKFIRNYR
                     LQKMKEKLF"
BASE COUNT         2730 a         1281 c         1303 g         2544 t
ORIGIN      
        1 aaattaaaga cctaattatt aacctataaa aaaggcagga attgccgttc ctgcccttaa
       61 taaataagaa tcaaaacaat attaatccta aaaattaaaa taaagttatg aaaaaaacta
      121 tactcttctg tattttactt gtggctacaa aagcatttac gcaaacgatt cctgcaaatg
      181 aagtgagttt agaccaaatg ctcgtgaata ttgataaaac aaccgtaact agcgggatta
      241 tttatgaacg tatagctcca tttaccaatt tgtataattt taacacaaaa acaaatcgaa
      301 atatagccga ttttattctc tttaaacaag cacttttaga aatgtatctt gctagtaatc
      361 aattacaatt cagctcggtt gagacactaa ataataatct aaatccaatt ttatatgatg
      421 atgcttctgt ttacatggga gttctaaata ctccttttca aagtgtcaat tacaatgaag
      481 aaaatccttc gttagggagg ctgcaatatg acgaaaccaa ttttttgttt tatgcttcaa
      541 caaatacaag ttatcctttt ttcaactcct cttttgcgac agtaatttct ccacttaaag
      601 atgttgtaaa aggagattat atttctttta attgggctag tagttttatt ttcgaaaaca
      661 ccctcaatag cactaaaaaa ataaaaaccc tgacagtcaa ttacggaacc ggaatagatt
      721 atactgtaat ttcaaattat gtcatagcta gtgcaacaca acaaataacc tatcctgaca
      781 ctggtgaaaa acaacttaca tttactgtaa cttactctga caacagttcg ctaacaactt
      841 atgccaaaat aaaataccta aaagtggtta atgatccttt acatagaata atgggtagtg
      901 atccaaattg cggtactgac tatggattga cagaaaatag aacatctact tcagaatatg
      961 cttttcaagg atatgacgaa ccttatgctt ttaaaggatt tattaattat aaagtttttt
     1021 ataaaacaaa tggaacccaa aagataacga aacccattat cattatagat ggttttgacc
     1081 ctggagacaa acgccaagtt ttgccttgtg attatgaacc atttgaatat aaagtgggtg
     1141 aaagtcgggc aatttcagat gtgatgaaat atggcgttaa taaagagaat gatttaatta
     1201 aggagctcca atataaagga tatgatgtgg ttattgtaaa ccaaccaact tattacataa
     1261 tgactacacc gccttatcaa atagtgccac gaggaactgc aggaagtagg gaaatagatg
     1321 gcggtggcga ttatatagaa agaaatgggt tgaatttagt ttcgctaata caaaaaataa
     1381 ataccgaatt aacaaccaac ggcagtaccg aacaactagt aatcgtaggg ccgagtatgg
     1441 gagggcaaat ttcaagatat gctcttgcct atatggaaaa aaataatatt cctcacaaca
     1501 cacgactatg ggtaagtgtt gacagtcccc atcttggggc aaatatcccg cttggtgctc
     1561 aagcagaaat caatttgtta aaaagcagca atgatcaagc aaaagatttt tatgataagc
     1621 aattgggatc agtagcagca agacagcaac ttattgaatt tcataaagaa atagttacgt
     1681 atgttacttt tggtacttta acagtgccat tattacgaac agttgaccaa tcattattaa
     1741 acgcaagaac aatttctcaa ggatttagta ccaattctgg ctcatcattt tttcaaaatt
     1801 tctataataa tcaattctca aatggcttac ccaattcaaa agggtatcca atgaatttga
     1861 gaaaaattgc cttagtaaat ggttcactta caggtagtaa aacaggttta gacagtcaac
     1921 aaacaatgaa tatacgtgga tttgcagata tatgtttacc attttgtttt aaagttcatg
     1981 ccgcgagtat ggaagtttat actatgccga tttttgggaa tacctcaaca atagcaagat
     2041 tcaaatccgc atttacagat aaaaacacct ttgctccgaa tttaaattct cgtggtaata
     2101 tggatataat acctggtggt tattttgata gttataacca attgaataat tcaattatgg
     2161 gacaatcagg tcccactaca aattttgcaa taccttttca tattgactta agtgcaaagg
     2221 atataaattt tgaatcacga accaatacaa aaatacattc ttttattcct acattttcag
     2281 cattaggaat gaaaaatcca gatcaagatt tggggtcaaa gtttaaacag aaatttagtt
     2341 tgtagcggag aaactccatt cgatagctat tatggagaaa cagacaatac agaacacaca
     2401 tccttcaatt acaccagtgt agcttggttg tttaaagagt taggggataa cattaatcct
     2461 ccaatagctc aagcaccaac atttccatta agtaattcaa aactttctgg aatagataaa
     2521 atatgtgtgg gtcaaactag cacttatagt tttaccgata tatgcaaaat accaagcgct
     2581 gtaacgtgga gtgtatcttc taatctacag attatgagtt ttacagctac aagtattatt
     2641 attaaaggtc ttactagtga tcaaggagta gtaacagcaa cttttcagaa tgggatgtcg
     2701 gtttcaaaaa atgtttgggt aggcgttcct gctttcaatt tgcttaggac aagtatagaa
     2761 acctgtgatg gtataaaata ccattatgta aactttgaaa taaatcctcc tccaggatct
     2821 ggaacaactt ttaaattctt ttatccaaat gcaaatgtaa cttacactat gacttcttat
     2881 ggtaagtatc aattcagatt tgttgaaaca tttgatgatt tttttgatat tgcagtaaca
     2941 gcaactaaca gttgcggtac atattctatt tatagcgaag aatatattag tagttgtact
     3001 ggaggagctt tgagcagatt aaatgctcca actaaaacaa aaaaatataa aatttatcca
     3061 aatccaacaa gcgatataat aaatatttcg ctagtagatg aagcacaaaa acctgttaca
     3121 acctcaaaaa ttgtagctga actatatgat tttacaggac aaccaaaata caaagtagaa
     3181 gttaaaaata atattgcttc tataaattgt aatggtcttt caagcggtat ttatatttta
     3241 aaaattataa ttgatgggaa cgtcgaaagt catcaagtat tcgttcaata agcaatactg
     3301 aatatattta agaaacagcg gttttagcat tggtctgaaa ccgctgtttt tattttctgt
     3361 tttccgccaa atgatttaat tataattaca tttactcaat gataaagaaa ctaatattca
     3421 tatcactttt attcctttct cagttttcaa atgcacagaa atatgtggat ttattctcca
     3481 tcaattatgg aaaatctgcg gtgactagct atgaaaatgg ctctcaatca acaacaatta
     3541 caaacataga tattgatttc accttgccaa taaagttgga tgaaaaaaat gcgattatta
     3601 ccggaataga ttttagtaat aatatcttgc agttatttcc tgatgcaagc tataacaatc
     3661 tatacttaac gagaataaag acaggaatta atatcattca ttctgagcat tggagcggaa
     3721 cttatatttt tcttcccaaa atatcttctg attatataaa tgttagtatg gaagattttt
     3781 atataggagg tttggtagtt ttgaaatata gaaaaaggaa taatttaagt tataaattcg
     3841 gactttatgc tggtaatgaa gcttttggat tgtttatttc gcctgtaatc gggctgtatt
     3901 atttgagccc aaactctagt tttgaaatga atttgtattt gccaagcaat gccgatataa
     3961 attatagttt aactaataaa acaaaagcag gaattgattt tgttgcacga ggaaaaagtt
     4021 acaaactcac tactgatgat gttcgttcaa gttatgctga aaacaattct ttagaatttt
     4081 catcctattt acaaaataat tcactcaata aaaacatgct tattcgctta aaattgggat
     4141 tttcgaccaa tagttttgaa gtttatccca ttgaccaaaa aataactttt gcaacaactc
     4201 tattaaaatt tggagatcac agaacccaat taaacagtac tttatcaagt agtcctttca
     4261 tcaagataga ggcaatatat cggtttgata ttcctactag taaggaaaaa taattaaaaa
     4321 agtgtagaga gattcagttt ctgaattaaa attgttaacc cgattcttta tttttccgta
     4381 ttccatcaaa tgatttacta tttttgtaaa caatagaaat tgtaatttaa aaataaaatg
     4441 aacaaaaatt attacgcaat tataatggct ggtggagttg gatctcgatt ttggccagta
     4501 agtacagcag agtttccaaa acaatttcac gatatgcttg gggcagggga aaccttaatc
     4561 caaaagacct tcagtagatt gtcgaaattg attccagtgg aaaatatatt ggttttaacc
     4621 aatgaaaaat acaatgctat tgtattggag caattgccat tggtaaaaca agagcaagtt
     4681 ttgttagaac cagccatgcg aaatacagct ccttgtattt tatatgcttc gctaaaaatt
     4741 caaaaacaaa atccagatgc tgttatggtt gttgcaccaa gcgatcactg gattgaagac
     4801 gaaaatgaat ttgcagccaa tttaaagcaa tgctttgatt tttgcgccaa tgaaaatgcg
     4861 ttgatgacac ttggaattca acccacattc ccaaacactg gttttggata tattgaatat
     4921 gacaaaaccg atgctaatcc aatcaaaaaa gtgtcgcaat ttcgtgaaaa accagattat
     4981 gaaactgcaa aaacattctt gcaaagcggt aatttcttgt ggaatggtgg tatttttatt
     5041 tggagtgcaa aatcgataac tgaagccttc gaaaaatttc aaccacagat gaacgccttg
     5101 tttcttaaag gtttagaaag ttataatact cctgccgaga aaactttcat cgaagagaat
     5161 tatgctttat ccgaaaatgt ttctattgat tatgccgtga tggaaagcgc caaaaatgtt
     5221 tatgttcttc cagctacttt tgattggaac gacttgggaa cttggggtca gcttcacgaa
     5281 aaactagata aagatgagaa taaaaatggg gttattaatg caaaagttgt cttagaaaat
     5341 gcctcaggta atatcatcag gtctgatgcc gataaaatca tcgttgttga tggtttgcac
     5401 gattacatta ttgtggacaa agagggagtt ctacttattt accctaaaag taaagagcaa
     5461 gatataaaac gaataacaac tttggcaaat aaactttaat ttagttattc atttataggt
     5521 gtattcagtt cgtataaaag actatgcaaa tttaaaaacg ggttctatct aaaaaaaata
     5581 tttagataga acttttttta ttccttcaaa taagcttctc ttaccttttt gaacaaattt
     5641 gaggaataaa cgaagtcaac aacagcttcg ttgtctgttc tgaaaatttc ttcttttgtg
     5701 ccttcccatt cttttaaacc atttttaaga aagacaattt ttttgccaat ttccatcaca
     5761 gaattcatat cgtgggtgtt gattactgtc gtgatgttat actcttcggt aatttctcgg
     5821 ataagattat caattagagt tgccgtgttt gggtctaaac ctgagttggg ttcgtcacaa
     5881 aaaagatatt ttggattgtt cacaatggct ctagcgatgg caacccgctt ttgcattcct
     5941 cccgatattt cggagggtaa tttattatga gcgccaacta aagcgactct ttctaaaaca
     6001 aaatcaacac gctcttttat cttttttttg ttgtcgtttg tgaacatttt taatggaaat
     6061 ccaacatttt cagatacggt catcgagtca aaaagggcgc ttccttgaaa aagcattcct
     6121 atttcggttc gcaattcgcg tttttcatcc gagttgaggt cggaatatat tctgccatca
     6181 aaagaaattg ttcctacttc aggagtgtga attccgagta gtgatttcaa taaaacggtt
     6241 ttccctgaac cactttgccc aataattaaa ttcgtttttc cagcttcaaa aattgtagaa
     6301 acgcctttaa gaattttggt ttcaccaaat gatttttcta tgtttttaat ttctatcatt
     6361 aggttaaaag taattgtgtt agtatataat tcactaaaat aattaaaacc gatgtccaaa
     6421 caaaggcaac ggtacttgct ttccctactt caagtgcgcc acctttcata taatagccgt
     6481 gaaaagcagg aatagacgct agtaatattc caaaaatgaa agttttaata aaggcatagg
     6541 taacgtgaaa aggttcgaaa ttaatttgta aaccatcgat aaaatctgaa ctagcaccaa
     6601 acccaccgta aactgctgca atatacccac caaaaacgcc cagaaacata ctaattccaa
     6661 ttacgaatgg atacaaaaga acagcaatta gtttggggaa aaccaaataa tttaaggagt
     6721 ttactcccat aacttctaag gcatctattt gttcggttac tcgcatagac ccaatacttg
     6781 atgtaataaa ggaacccatt tttccagcca taattaccga aataaaagtt ggtgcaaact
     6841 ctagaattac cgattctcgt gtggcataac caataagagt tttgggaatc aaagggttat
     6901 ttaaatttaa tgcggtttga atagaaacaa caccgcctac aaagaaggaa atgaaagcca
     6961 ctattcctaa ggagtcaata attaaatcat caatttcctt gaagatgagc cctctcatta
     7021 cagaccattt ggtttgtttg ctgaaaattt ctttccacat caagaagtat tttccgattt
     7081 gggataaata gcgaagaagc atcataattt ttaaatattg tcaaaaatac taagaagttg
     7141 tgagttataa gttatgagtt atgagtttta acttaaaaca atttctcctt cattttctgc
     7201 aaacgatagt ttcttatgaa tttggcttgt tcgggattaa ctaacattgg ttttttggct
     7261 gatttggctt tccaaaaacc ttgaatgtaa tcaaaaaaaa gtaaaggttt tttcttcatc
     7321 attgccaatt tagccgatgc aatggaggtt atccaaaagc cgtaaccaag agtgtaaaaa
     7381 gcttcacctt gtttgtatcg ggctgttttg ttataattgg ctcccgttgg tttaaggtgt
     7441 tttacgtgta aactttcgtc tgtaactact ttccaattgt aatatttaca aagtagttca
     7501 tctacagtgt cccaacccat tgcgggtttt agtccgccaa tttgtttgaa agttgctgta
     7561 cgataagctt tcaatgcgcc tctaatgtga tctttgtcgg tcaggttttc cagaatccaa
     7621 ttgccatttt tttcgatata acaaaatccg ccaaccattc caattcgttc atctgattga
     7681 aaatgtttaa ttattgtttc aaaataattg gttggaaaaa ttaaatcggc atctattttt
     7741 acaattaaat catagttttc gtctaatgtt tcgaatcctt tctggaaagc ttgaatgact
     7801 ttacttccag gtaaatggat gttttcggaa gttttgttta ccaattgaat ccaaggat
//
LOCUS       DAOSNN010000111         7739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9414_length_7739_cov_5.083420, whole genome shotgun sequence.
ACCESSION   DAOSNN010000111 DAOSNN010000000
VERSION     DAOSNN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9414_length_7739_cov_5.083420"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..540
                     /gene="aroQ"
                     /locus_tag="PLQ70_06915"
     CDS             127..540
                     /gene="aroQ"
                     /locus_tag="PLQ70_06915"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQE34385.1"
                     /translation="MKIGIINGPNLNLLGKREPEIYGSQTFEDYLEILKTKFPQIELT
                     YFQSNIEGELIGKIQEFGFSYQGIILNAGAYTHTSIGIGDAIKSITTPVVEVHISNTF
                     SRESFRHQSYISGNAKGVILGFGMKSYELAIQSFL"
     gene            639..3125
                     /locus_tag="PLQ70_06920"
     CDS             639..3125
                     /locus_tag="PLQ70_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQE34386.1"
                     /translation="MKKIYLLLFLLVSVFSVAQIKGTITDNKGIPLSLVTILEENTYN
                     GTSSNEKGNYELNIKKTGKHTLIFQYLGYKTQKITVNIDKFPFTQNIQLIEENLSLSE
                     VVINSKDNPANAVIKQAIAHKKENSEKTARFKADFYSRGIFKVKDLPKKILGQKIGDL
                     DGAVDSTGTGIIYLSETVSKIIFEKPDNLKERIIASKISGDNKGFSYNTARSTIYDFY
                     DNTLDFNSKMISPIADNAFNYYKYKLEGTFQDENNLMINKIKVIAKRDAEPVFEGYIY
                     IVEDSWAIYAVDLDIKGYRMHQEFVDVMKLKQNFSYNKNNQIWAKNTQSLEFSAGVFG
                     IKFNGKFSYVYSNYEFEKAFAKKTFTNEIVSFEANSNKKDSAFWNKIRPIPLTIEENT
                     DYIKKDSVHTVRNSKKYLDSIDKKGNKFKFLSPITGYSWKNSSEKKSFRYDGLLNLSS
                     LSFNTVQGWNLDSGFSFRNWKQQEEKGKSTSISTKFNYGFSDDRLRVTADYYHRFNNQ
                     NYATLSVFGGTKVNQFNPEEPISSFINTVSTLFFEDNYMKLYNKEFAGATYGQDVGNN
                     FYLKGTLEYQQRKPLFNTTDYTFIQSDDVYSSNNPLLPDDNLTPAFEQHHLTKASLFA
                     RINFGNKYISRPDGKLNIRNNNYPTLYFGYENAFASNESKYEYQLISSQIRYDFNAGN
                     KGTLGINLKAGTFIHGDNISFVDYKHFNGNRTHIGTSERYLNVFNLMPYYSNSTNKEY
                     FEAHLEHNDKGYIMNKIPLLNKLNSTLVLGFHALAVPDTKPYTEFTVGLDNLGFGKLK
                     VFRFDYVHSYQNGIQENGVVFGLKILNVLE"
     gene            3208..4149
                     /locus_tag="PLQ70_06925"
     CDS             3208..4149
                     /locus_tag="PLQ70_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34387.1"
                     /translation="MSDYEQTINELPVILKKSQMRTFFLIITLFCLQKIFADQPRIRY
                     SFKSPNLRFELKPSDTIFSDKKVYTDSIYDSKTKTYYKSSYTFPDRYYWGLYDTQTKQ
                     KLYTIKNDSLFIEMKTAVISDDGQNIVIIDDYAGGFGFKEIEMMTFYEKDNRIKRLKL
                     GDLIQNLCALSYSVSHMTWCSRNFKFNDRKEITIETYEFYNYTFNKNGELINKKSDAL
                     IEPNDTLAVGEIKRVGRNKYNVKVNFCIRGKLKTDEILEIECKDSEMKKIYGKLYGFL
                     KSKNKAMTKEFTRTFLIKDSKIQKVNFPIPTYNSQSH"
     gene            complement(4328..5200)
                     /locus_tag="PLQ70_06930"
     CDS             complement(4328..5200)
                     /locus_tag="PLQ70_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQE34388.1"
                     /translation="MNKEQTAVKATYFSIASNTLLALIKGLAGFFGHSYALTADAIES
                     TTDIFSSFLVLFGIKYSNKPADDNHPYGHGRAEPLVTFLVVGFLITSATIIAHESITN
                     IGVPHKLPESWTLIVLGTIILWKEYSFRLVMKRSKEANSSSLRADAWHHRSDAITSVA
                     AFIGISIALFFGKGYESADDFAALFASGFILYNSYKIFRPALGEIMDENLYDDLIVEI
                     RKVSLQVEGVESTEKCFIRKAGMKYHVDLHAIVNANITVREGHDISHLLKDTLRAEIP
                     ELGHVLIHIEPSYY"
     gene            complement(5270..6580)
                     /gene="murA"
                     /locus_tag="PLQ70_06935"
     CDS             complement(5270..6580)
                     /gene="murA"
                     /locus_tag="PLQ70_06935"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQE34389.1"
                     /translation="MGVFKIEGGVPLKGEITPQGAKNEALQILCAVLLTPEKVIINNI
                     PDIIDINKLITLLGNLGVKIQKNGPGSYTFQADEVNVGYLETEAFKKEGGSLRGSIMI
                     VGPLLARFGKGYIPKPGGDKIGRRRLDTHFEGFINLGAKFRYNKEDHFYGVEAPEGLT
                     GAAMLLDEASVTGTANIVMSAVLAKGTTTIYNAACEPYLQQLCKMMNAMGAKVSGIGS
                     NLLTIEGVESLGGCTHRILPDMIEIGSWIGLAAMTRSEITIKDVSWENLGLIPNVFRK
                     LGITLEKKGDDIYIPAHTDGYQVKTDIDGSILTIADAPWPGFTPDLLSIVLVVATQCK
                     GDVLIHQKMFESRLFFVDKLIDMGAKIILCDPHRAVVIGHDFKSRLKATTMSSPDIRA
                     GISLLIAALSAKGTSTIQNIEQIDRGYERIDERLRAIGAKIVRA"
     gene            complement(6677..7402)
                     /locus_tag="PLQ70_06940"
     CDS             complement(6677..7402)
                     /locus_tag="PLQ70_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HQE34390.1"
                     /translation="MNTKYIKEVANDVVHHLEYNAERTHLIIPEYGRHLQKLIDQATE
                     IEDREERNKAAKYIIQVMGSLNPHLRDVPDFQHKLWDQIFIMSDFKLDVESPYPVPTR
                     EVLQLKPDVLKYPQNFPKYRYYGNNIKYMIDVANKWEEGEMKNALVKVIANHMKKSYL
                     SWNKDTVKDDVIFEHLYELSGGKINMLQSTEELLTTTDLLRTNKRVSNKITPAGQPKI
                     QSNKNSKLGKSNPTHKNQNRKPL"
     gene            complement(7405..7701)
                     /locus_tag="PLQ70_06945"
     CDS             complement(7405..7701)
                     /locus_tag="PLQ70_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HQE34391.1"
                     /translation="MDSEKEKETQEFYERLKKELDLSTAWPALYLYKFIVPTDKENIL
                     RVEEAFDCMGAVIKTTKSKTGKFTSISVDVMMKNSQEIIEKYIELSTIKGIISL"
BASE COUNT         2626 a         1367 c         1262 g         2484 t
ORIGIN      
        1 ccgatgccaa aaactcggta cttcaacttt cggcaggact tatgttttaa caaaatttaa
       61 aattattcgt gatttaaaac cgttctttat tggaacgttt tttttatttt tacgaaaatt
      121 atttttatga aaattggtat tatcaacgga cccaacctca acttattagg aaaacgcgaa
      181 ccagaaattt acggttcaca aacttttgaa gattatcttg aaatcttgaa aactaaattt
      241 ccacaaatag aattaacgta ttttcaaagc aatatcgaag gcgaattgat aggtaaaatt
      301 caagaatttg gtttttctta ccagggcatt attctcaatg ctggtgctta cacccacact
      361 tctattggca ttggcgacgc cataaaatca ataacaactc cagtggttga agtgcatatt
      421 tcgaatactt tttcgagaga aagtttccgt caccaatctt atatttcagg aaatgccaaa
      481 ggagttattc ttggttttgg aatgaaaagc tacgaattgg caatacaatc gtttttgtaa
      541 ttctaatcat ttctttacaa tctctttagc atttaaataa caaaattttg tgattgtaat
      601 tctttcctac ttgtatattt gtaagaaaaa attacggtat gaaaaaaatt tacttgctcc
      661 tttttctttt agtttcggtt ttttcggttg cacaaattaa gggaactatt actgataaca
      721 aaggaattcc gctttcgctc gttacaattc tagaagagaa cacctataac ggaacttcct
      781 caaacgagaa aggtaattat gaattgaaca tcaaaaagac aggaaaacac actctaattt
      841 ttcaatattt aggatacaaa acccagaaaa taacggtcaa tatcgataaa tttccgttca
      901 cccaaaacat acaattaatc gaagaaaatc tgtcgctttc tgaagttgtc atcaactcca
      961 aagacaatcc cgccaatgct gtaatcaaac aagccattgc tcacaaaaaa gaaaactccg
     1021 aaaaaacagc gcgtttcaaa gccgattttt attcccgtgg gatttttaaa gtcaaagatt
     1081 taccaaaaaa aatattggga caaaaaatag gcgatttaga tggcgcagtc gattcaactg
     1141 gaacaggaat tatctattta tcagaaacag tttcgaaaat tatttttgaa aaacccgaca
     1201 atttaaaaga acgaattatc gcttcgaaaa ttagcggaga caataaaggc ttcagttaca
     1261 acacagctcg ctcgacgatt tatgattttt atgacaatac tttggacttc aacagcaaga
     1321 tgatttcgcc cattgccgac aatgctttca actattataa atacaaatta gaaggtactt
     1381 ttcaggacga aaacaacctg atgatcaaca aaattaaggt tattgccaag cgagatgccg
     1441 aaccagtttt tgaaggttac atttatatcg tggaagattc ttgggctatt tacgccgtcg
     1501 atttagacat caaaggctac agaatgcacc aagaatttgt ggatgtaatg aagttaaaac
     1561 aaaatttcag ctacaataaa aacaatcaaa tttgggccaa aaacacacaa agtcttgaat
     1621 tttcagcggg ggtttttggc ataaaattca acggaaaatt ctcttatgtt tacagcaatt
     1681 acgaattcga aaaagcgttt gccaaaaaga cttttaccaa tgaaatagta agttttgaag
     1741 ccaattccaa taaaaaagat agtgcttttt ggaacaaaat cagacccatt cccctgacca
     1801 tcgaagagaa taccgattac atcaaaaaag atagcgttca tactgttcga aactccaaaa
     1861 aatatttaga ctctattgat aagaaaggaa ataaattcaa atttttgagt cccattactg
     1921 gttattcttg gaaaaatagc agcgagaaaa aatcattccg ttacgatggt ttgctcaatt
     1981 tatcttcgtt gagtttcaac accgttcagg gttggaatct ggattctgga ttttcttttc
     2041 gaaattggaa acagcaggaa gaaaaaggaa aatcgacttc catcagcacc aaattcaatt
     2101 acggattttc agacgataga ctgcgagtta ccgccgatta ttaccatcga ttcaacaacc
     2161 agaattatgc aacactatcg gtttttggcg gaacaaaagt caatcaattc aatcccgaag
     2221 aacccatttc aagtttcata aacaccgtaa gcaccttatt ttttgaagac aattatatga
     2281 aattgtacaa caaagaattt gctggtgcta cttacggtca agatgttggc aataattttt
     2341 atttgaaagg aactttagaa tatcagcaac gcaaaccttt gttcaacact acggattata
     2401 catttatcca aagcgatgac gtttacagtt cgaataatcc gttgttgcca gacgacaatt
     2461 tgactcctgc attcgaacaa catcatctta cgaaagcttc cctttttgcg agaataaatt
     2521 tcggaaacaa atacatttca aggccagatg gaaaactaaa catcagaaac aacaactatc
     2581 ctactctata ttttggctat gaaaacgcct ttgcctccaa tgaaagcaaa tacgaatacc
     2641 aattgatttc gagtcaaatt agatatgatt ttaatgcggg aaacaaggga actttaggaa
     2701 tcaatttgaa agccggaact ttcattcacg gtgataatat ttcgtttgtt gattacaaac
     2761 atttcaacgg aaatagaaca cacatcggga ctagcgaacg ctatttgaac gttttcaacc
     2821 taatgcctta ttattcgaat agtaccaata aggaatattt tgaagcgcat ttagaacaca
     2881 acgacaaagg ctatattatg aataaaattc ctttgttgaa caaattaaat tcgactttgg
     2941 ttttaggatt tcacgcactt gccgttcctg acacgaaacc ttacacagaa tttacggtgg
     3001 gtttggacaa tctaggtttt ggaaaattaa aagtatttcg ctttgattat gtgcattcct
     3061 atcaaaacgg aatccaagaa aatggagttg tttttggttt gaaaattttg aatgttttgg
     3121 agtagaattt ttggatgaat ttagctatat ttgagaataa ataaacacta acgtttatca
     3181 gacaagtcga accaatttag gggtgatatg tctgattatg aacaaactat aaacgagtta
     3241 ccagtcattt tgaaaaaatc acaaatgaga acattctttt taataattac attgttttgc
     3301 ttacagaaaa tatttgctga ccaacctaga attaggtatt ctttcaaaag cccaaattta
     3361 agatttgagt taaagccttc cgacacaata ttttcagata aaaaagttta tacagattca
     3421 atatatgact ctaaaacaaa aacttattat aaatcgtcat atacatttcc tgacagatat
     3481 tattgggggc tatatgacac acaaaccaaa caaaaacttt atacaataaa aaatgacagt
     3541 ctatttatcg aaatgaaaac cgctgtaatt tcagatgatg gacaaaacat tgttattatt
     3601 gatgattacg caggtggttt tggctttaaa gaaattgaaa tgatgacatt ttacgaaaaa
     3661 gataaccgaa taaaacgtct taaacttggt gatttaattc aaaatttgtg tgccttaagt
     3721 tattctgtaa gtcatatgac atggtgttca agaaatttta aattcaatga cagaaaggaa
     3781 attacaatag aaacttacga attttacaat tatacattta ataaaaatgg cgaattaatt
     3841 aataagaaaa gtgacgcatt aattgaacca aacgacacac tagccgttgg agaaataaaa
     3901 agagtaggaa gaaataaata taacgtgaag gttaactttt gtataagagg aaaattaaaa
     3961 actgatgaga ttttagaaat agaatgtaaa gacagtgaaa tgaaaaaaat ttatggaaag
     4021 ctttatggtt ttttaaaatc aaaaaataaa gcaatgacga aagaattcac acgaacattt
     4081 ctaataaaag actcaaaaat tcagaaagta aattttccga ttccaacata taactcacaa
     4141 agccactgaa atattataaa ttcacaagaa taaaacgctg gttaactgct actgcaaagg
     4201 aattgggcaa ttggctttcc cgaagtatag ggaggaaagt tggttttgca tatgggatga
     4261 ttagcaaatc cgaaaacagg gtttcattta gtccttgtgt ttaataaaac catttcaact
     4321 gaaattctca ataataagaa ggttcaatat gaatcaaaac gtgtcctaat tcggggattt
     4381 ctgctcgcaa agtatctttt aacaaatggg agatgtcgtg accttctctc accgtgatat
     4441 tggcgttgac aatggcgtgc aaatcgacgt ggtatttcat tcctgcttta cgaataaaac
     4501 atttttcggt gctttcgaca ccttcaactt gaagggaaac tttccttatt tcgacaatta
     4561 aatcatcgta taaattttcg tccattattt cgcccaaagc gggtctgaaa attttataac
     4621 tgttatacaa aataaatcca gaagcaaaaa gtgctgcaaa atcatcggcc gattcgtagc
     4681 ctttcccaaa aaataaggca attgaaattc caataaatgc cgcaaccgaa gttatggcat
     4741 cgcttcgatg gtgccaagca tcagctctta gcgaagaact attggcttct ttgcttcgtt
     4801 tcattaccaa acgaaaggaa tattctttcc ataaaattat agttccaaga acaattagcg
     4861 tccaagattc aggcaatttg tgtggaactc caatattggt aatgctttcg tgagcaataa
     4921 tggttgccga agtaatcaaa aaaccaacta ccaagaaagt taccaatggt tcggctcgac
     4981 cgtgtccgta aggatgatta tcgtcggcag gtttgttcga atatttaatt ccaaataata
     5041 ctaaaaatga agaaaaaata tcggttgtcg attcaatagc atcggcagtc aaagcatagg
     5101 aatgtccaaa aaaacctgcc aaccctttta ttaaagctag taatgtatta cttgcaatac
     5161 taaaataagt cgcttttact gctgtttgtt ctttattcat ttggataatt ataaaaacct
     5221 caaaagcttg tgcaactttt gaggtttgaa gtttttttta aaatatgaat taagctctta
     5281 caatttttgc tccaatggct ctcaatcgtt cgtcaattcg ttcgtaacct ctatcaattt
     5341 gctctatatt ttgaatggtg ctggttcctt ttgctgaaag tgcagcaatc aacaaggata
     5401 ttcccgctct aatatctggc gaagacattg tcgtagcttt tagtctggat ttgaaatcgt
     5461 gaccaataac taccgctctg tgtgggtcac aaagaataat tttagctccc atatcaatca
     5521 atttatctac gaaaaacaaa cggctttcaa acattttttg atgtatcaaa acatctcctt
     5581 tgcattgggt tgccacaaca agaacaatac tcaataaatc gggagtaaat cctggccaag
     5641 gcgcatcggc aatagtaaga atggagccgt cgatatcggt ttttacttgg tatccgtcgg
     5701 tatgagctgg aatgtaaata tcgtcgcctt ttttctctaa agtaatgccc agttttctga
     5761 agacatttgg aatcaatccc aaattttccc agcttacatc tttaatagtg atttcgcttc
     5821 tggtcattgc tgccaatcca atccagcttc caatttcgat catatctgga agaattctat
     5881 gagtacaacc tccaagactt tcaacccctt ctatagtcaa taagttagaa ccaatacctg
     5941 aaacttttgc tcccatagca ttcatcattt tgcacaattg ttgcaaataa ggttcgcaag
     6001 cagcgttata aatggttgtt gttcctttag ccaaaaccgc tgacatcaca atattggcag
     6061 ttccagtaac tgatgcttcg tctaacaaca tagcagcgcc agtcaaacct tctggagctt
     6121 ctactccata aaaatggtct tctttattat aacggaattt tgcacccaga ttgataaatc
     6181 cttcaaaatg cgtatctaat cttcttcgtc caattttatc accacctggt ttaggaatat
     6241 atccttttcc gaaacgtgcc aaaagtggtc caacaatcat aatagaacct cgaagtgagc
     6301 ctccttcttt cttgaaagct tccgtttcta aatagccaac attgacttca tcagcttgaa
     6361 aagtataaga acccggtcca tttttttgaa ttttaacccc taaatttccc aaaagggtaa
     6421 ttagtttgtt aatgtcaatt atgtctggaa tattattgat gattactttt tcaggtgtca
     6481 acaatacggc gcataaaatt tgcaatgctt catttttggc tccttgtggc gtgatttctc
     6541 cttttagagg aacaccacct tcaattttaa aaactcccat atttgttttt agttatgagt
     6601 tataaattat gagttttaag ttaagtatga attataagtt agttttgaaa attcataact
     6661 cataattcat aattccttat aaaggttttc tgttttggtt tttatgagtc ggatttgact
     6721 ttcctaattt actgtttttg ttgctttgaa ttttgggttg accagcagga gtaattttat
     6781 tggaaactcg cttgttggtt cgcaataaat cggtggtggt caaaagttct tctgtacttt
     6841 gcaacatatt tatttttcca cctgacaatt catataaatg ttcaaaaata acatcgtctt
     6901 ttaccgtgtc tttgttccaa ctcaaatagg attttttcat atgattggca attactttta
     6961 ccaaagcatt tttcatttcg ccttcttccc atttattggc aacatcaatc atatacttaa
     7021 tattattgcc ataatatcgg tatttcggaa agttttgtgg atattttaaa acatccggtt
     7081 tgagttgtaa aacttcgcga gttggcactg gataaggaga ttctacatcc aatttaaaat
     7141 cggacatgat aaagatttga tcccataatt tgtgttgaaa atcaggaaca tctcgcaaat
     7201 gaggattcaa actccccata acttgaatga tgtatttagc tgctttgttg cgctcttctc
     7261 tatcttcaat ttcggtagct tgatcaatca atttttgcaa atggcggcca tattcgggaa
     7321 taattagatg agttcgctca gcgttgtatt ccaaatgatg cacaacatcg tttgccactt
     7381 cttttatgta tttagtgttc atatttatag cgaaataata cctttaatag tagataattc
     7441 aatatatttc tcaattattt cttgagaatt tttcatcata acatcgacag aaatgctagt
     7501 gaatttgcct gttttggatt ttgtggtttt aatcactgcg cccatacaat caaaagcttc
     7561 ttcaactcga agaatatttt ctttatcagt aggtacgata aatttataca aatacaatgc
     7621 aggccaagcg gtactcaaat ccaactcctt ttttaacctt tcgtaaaatt cttgggtttc
     7681 tttttctttt tctgaatcca ttctaaatta aaataaaagc aaagatacag ttttgattt
//
LOCUS       DAOSNN010000112         7726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9434_length_7726_cov_5.330465, whole genome shotgun sequence.
ACCESSION   DAOSNN010000112 DAOSNN010000000
VERSION     DAOSNN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9434_length_7726_cov_5.330465"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..696)
                     /locus_tag="PLQ70_06950"
     CDS             complement(118..696)
                     /locus_tag="PLQ70_06950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HQE34392.1"
                     /translation="MKTEKEKMIAGEYYLAGDSVLIKERRKAKNLLHRLNVIEYRITK
                     KAREIIQELIPNAGTNLYIEPPFHCDYGYNIVCGDNVYFNVNCVVLDCAPVKIGSNVF
                     FAPNVQIYTATHPLDAELRKTLENALPISIGDDCWIGGNTVICPGVSIGNGCVIGAGS
                     VVTKDIPDNSLAVGNPAKVIKKLNSTQENTTD"
     gene            complement(737..1195)
                     /locus_tag="PLQ70_06955"
     CDS             complement(737..1195)
                     /locus_tag="PLQ70_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQE34393.1"
                     /translation="MKKSIFLLVLLSFGLASFAQETASNYDEKLAKSLNADERGMKKY
                     VFCLLKTGSNTTATKEESQKLFEGHMTNIGRLAKEGKLVVAGPFMKNDRNYRGLYIFN
                     VETIDDANLLVASDPAVKANLLEAELTLWYSSAALQETLKIHDKITKPKM"
     gene            1371..3509
                     /locus_tag="PLQ70_06960"
     CDS             1371..3509
                     /locus_tag="PLQ70_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE34394.1"
                     /translation="MKTFLSIALFFCSIISFSQTTISGKIVDEKNKPVPNANIYIDGT
                     YDGATSDDKGDFSFITTAVGNQNLVISALSFETQSLPIVVENFKNQTLQLKPSSTQLN
                     EVLITAGTMESGGKSRVSVLKPLDILTTAGNPGDIVSAFKTLPGTQVVGESGKLFVRG
                     GEANETQTFIDGIRVAQPYIQTANNVPTRSTFSAMLFKGTTFSTGGYSAEYGEALSSV
                     LLMNTIDEPDLEKTDFGVMSVGASLGNTQKWKNNSLSVNTSYINLQPYQTLLPDNIEW
                     IKPYESFGGETVFRQKLDRGLLKFYASYTAANFDLKQDDVNYPDKLRTQNKNNNLYSN
                     LSYKGGFGDNWNIFAGISFAKSRNSGIFDVYNYDASENASHLKLKLIKPISDKIKTSF
                     GADYFYTNFSELVTNFKSGYNSNILAAYSETDFFIASNLVAKVGFRFSKNYLLNENSI
                     APRLALAYKVAKNQQFSMAYGTFEQAPKNDYLKYSTNLQAEKAQHYILNYEFNKDKQV
                     FKAEIYYKSYDNLVKYDTKSPVYSSNFNNNGTGYAKGLELYWRDNKNIKNLEYWISYS
                     YIDTERNYANFPNQATPSFVADHTASLVTKYWIQDWRSQIGATYTFSSGRPYNNPNET
                     VFMDGKTKTYNNLSVNWAYLISPQKILFFSVTNVAGIKNVYGYNYANTPNSAGIYESQ
                     AIVPTADRFFFVGFFWTISKDKKTNQLENL"
     gene            3633..4253
                     /locus_tag="PLQ70_06965"
     CDS             3633..4253
                     /locus_tag="PLQ70_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34395.1"
                     /translation="MTKIIIAIVFFITSLVSAQGQFEQGMGKAFQLWGEGKNTEASAL
                     FERIAAAEKTSWLPNYYVALVNTTTAFGTKDKEQINLLLTKAQNALDVELIKAPNNPE
                     LLVMQAMIHTAWIAYDPMTNGQKLSGTVMGLYAKAEAIAPENPRVVFGKAEFEIGGAK
                     FWGKDTKPMCAQIEKAIGLFATFKPETPFSPKWGLERAEAALKNCK"
     gene            4284..5621
                     /locus_tag="PLQ70_06970"
     CDS             4284..5621
                     /locus_tag="PLQ70_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQE34396.1"
                     /translation="MKNDYVKELPKAFFIGLTIFGVLNLIRFFNGNSIALDDKLVVMF
                     LYTMLYSFALHLANTYLFLFLDKIFSNERFSKRRIIIGFVSSFFLSLFVIFLLRLFIN
                     TIIENKSFSVFIADESASDYIVASIFTFVVLLIVHFVYLYKGYQENKVKEQKIIAGTA
                     NAKFESLKNQIDPHFLFNSLNVLSSLIEENPENAQRFTTSLSKIYRYVLEQKDKELVS
                     VEEELAFAKTYMNLLKMRFENSLFYELPTSILNPEAKVVPLSLQLLLENTVKHNVVSE
                     QKPLHIRIYIEGNYLAIQNDYQKKEVLQERKGVGLQNIINRYGIITNRKVLIEQNEQT
                     FTVKIPILTKQISIMETATYSDNTAYYRAKKRVEELKGFYGNLISYCCVIPVLIFINL
                     TYMPEFQWFWFSAAGWGFGLTMHAFKVFGYSSNWEERKIQEILRKEENKQTWK"
     gene            5638..5943
                     /locus_tag="PLQ70_06975"
     CDS             5638..5943
                     /locus_tag="PLQ70_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HQE34397.1"
                     /translation="MENKYQEEERYYEAKKRVEEIKGFYGNLISYIVVNIGLMVINLL
                     TSPKYLWFFWPMLGWGIGVFFHGMKVFNFMPFLGKDWEEQKIKEFMDKEKENKEIWK"
     gene            5960..6310
                     /locus_tag="PLQ70_06980"
     CDS             5960..6310
                     /locus_tag="PLQ70_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HQE34398.1"
                     /translation="MRRSRRYIDELESENYNPDDRYSLAYKRVKRIKGFYVHAMVYVL
                     INIFIVYGNYFGNSHKDYDFWSWQTFSTALFWGIGLLAHGMSVFGRNLFFGQNWEERK
                     IQEFMEKEKSEKWE"
     gene            6440..7198
                     /locus_tag="PLQ70_06985"
     CDS             6440..7198
                     /locus_tag="PLQ70_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQE34399.1"
                     /translation="MTTIIIEDEKPAARLLQRKLEKLNIEVGVMLHSVEESIEWFSKN
                     EHPDLIFLDIQLSDGLSFEIFEKVDIKSAIIFTTAYDEYALKAFKLNSIDYLLKPIDE
                     DDLEVAISKFKIRLPKPENTLQLDFEQIKKMFQNPFDKNFKTRFTVKIGQHLKVISTD
                     EIECFFSENKGTYLHTFDNRNYLIESTLEVLEQELDPKKFYRISRKFIVPLKSIKEIV
                     VYSNSRLKVILPTYKEDEVVVSREKVADFKAWIG"
BASE COUNT         2575 a         1241 c         1398 g         2512 t
ORIGIN      
        1 agccatggct tttttgggtg agacggaatt tccaaatgaa agtcgaaaaa gatttatttt
       61 tatgtttttt agcatatttt tatatgccaa aacgcaggtt attgaattaa tttgttgcta
      121 atcagttgtg ttttcttgag tgctgttgag ttttttaatc acttttgcag gatttcccac
      181 cgccaaggaa ttgtcgggaa tgtcttttgt aaccaccgag ccagctccaa tcacgcaacc
      241 atttcctata ctgactccag gacaaataac cgtatttcct ccaatccagc aatcgtcacc
      301 aatggaaatg ggcaatgcat tttcaagcgt ttttctcaat tcggcgtcaa gcggatgagt
      361 tgcggtgtaa atttggacat tgggtgcaaa aaatacgttc gaaccaattt ttacgggagc
      421 acaatccaaa acaacacaat ttacattaaa ataaacattg tcgccgcaaa caatattgta
      481 tccataatcg caatgaaacg gaggttctat gtaaagattg gttccagcat ttggaattaa
      541 ttcttgaatt atttctctag cctttttagt tattctgtat tcaatgacgt ttagtcgatg
      601 gagcaaattt ttggcttttc tgcgttcttt tattaaaact gaatcacctg caagataata
      661 ttctcctgca atcatttttt ctttttcggt tttcatagtt gattttttgg aaatagagaa
      721 atcatttggt tcaaaattac attttaggtt tggtaatctt atcgtgaatt ttcaatgttt
      781 cctgcaatgc cgcactgctg taccaaagcg tcaattcggc ttcaagaaga ttggctttta
      841 cggcggggtc agatgctact aaaaggttag catcgtcaat tgtttccacg ttgaaaatgt
      901 atagccctct ataattccta tcgtttttca taaatggtcc agccacaacc aactttcctt
      961 ctttggccaa ccgaccgatg ttggtcatat gaccttcaaa taatttttgg gattcttctt
     1021 ttgtagccgt ggtattgctt cctgttttca acaagcaaaa cacgtatttt ttcattccac
     1081 gttcgtctgc attaagggat ttggctagct tttcgtcata atttgatgca gtttcttggg
     1141 caaatgacgc caagccaaaa gacaacaata ccaataaaaa gattgatttt ttcatttttt
     1201 tgaaatttta agaataaaca aatttaagtt tttaaaggac taaatcttcg aatctatggt
     1261 catttttaaa cgtctttaca actcatcccc aaaattctac agttcggtta agtaaaattc
     1321 caatattcta atggttgaaa tacatttgct tcatcaaaaa aactaaaccg atgaagacat
     1381 ttttatccat agcccttttc ttttgttcca ttatttcttt ttcgcaaacc acgatttcag
     1441 gaaaaattgt ggacgaaaag aacaaacccg ttcccaatgc caacatttat atcgatggaa
     1501 cttatgatgg tgcaacatcc gatgataaag gagattttag ttttataacg actgccgttg
     1561 gaaatcaaaa tttggtcatt agtgctttgt catttgagac gcaaagccta ccaattgttg
     1621 tagaaaattt taaaaaccaa accctacaat taaaaccgag ttctacccaa ctaaacgaag
     1681 ttttaatcac ggctggaacg atggaatcgg gagggaaatc gagagtttcg gtattgaaac
     1741 ctttggatat tttgactact gcaggaaatc cgggcgatat tgtttctgct ttcaaaactt
     1801 tgccaggtac acaagtcgtt ggcgaaagcg gcaaactttt tgtccgtgga ggagaagcca
     1861 acgaaaccca aacctttatt gatggcattc gggtggcgca accttatatt caaacagcta
     1921 ataatgtgcc tacaagaagt actttttcgg caatgctttt caaaggaact actttttcaa
     1981 caggaggtta ttcggctgaa tatggtgagg cgctttcgag tgttttgttg atgaatacca
     2041 tcgacgaacc cgatttagag aaaacagatt ttggtgtaat gtctgttggt gcaagtctcg
     2101 gaaataccca aaaatggaag aataattcct tgagtgtgaa caccagttac atcaatttac
     2161 agccgtatca aactttgttg cccgacaata tcgaatggat taaaccttat gaatcttttg
     2221 gtggagaaac cgtttttaga cagaaattag acagaggatt gctaaaattt tatgcttcct
     2281 ataccgcagc caatttcgat ttgaagcaag acgatgttaa ttatcccgat aaattgcgta
     2341 cccaaaacaa gaataataat ttgtattcaa acctttctta taaaggcggt tttggcgaca
     2401 attggaacat ttttgcagga attagttttg ccaaaagtcg caatagcgga attttcgatg
     2461 tttataatta cgatgcttct gaaaatgcca gtcatttgaa gctgaaattg atcaaaccca
     2521 tttcggataa aataaaaacc agttttggtg ccgattattt ttacaccaat ttttcagaat
     2581 tggtaaccaa tttcaaaagt ggatacaatt cgaatattct tgccgcatat tcagaaaccg
     2641 atttttttat tgccagcaat ttagtggcaa aagtaggttt ccgattttcg aaaaactatc
     2701 tgttgaacga aaattcgata gcgccaagat tagctttggc gtataaagtg gccaaaaatc
     2761 agcaattctc tatggcttac ggaactttcg aacaagcacc caaaaacgat taccttaaat
     2821 acagcactaa tttgcaggcc gaaaaagccc aacattatat tttgaactat gaattcaata
     2881 aagacaaaca ggtttttaaa gccgaaatct attataaaag ctatgataat ttggtgaaat
     2941 atgacactaa atcgcctgtt tatagcagca atttcaacaa caacggaacc ggttatgcca
     3001 aaggtttaga gctgtattgg agagataaca agaatatcaa aaatctggaa tattggattt
     3061 cgtattctta tattgatacg gaacggaatt acgccaattt tccaaatcaa gctaccccca
     3121 gttttgttgc tgatcacacc gcttctttgg tgaccaaata ttggattcaa gattggcgtt
     3181 cgcaaattgg agctacttat actttcagtt ccggaaggcc gtacaacaat cccaacgaaa
     3241 cggtttttat ggacggaaaa acaaaaacct acaacaattt gagcgtgaat tgggcttatt
     3301 taatttctcc tcaaaaaata cttttcttct cggtaactaa tgtggctgga atcaaaaatg
     3361 tgtatggata caattatgcc aatacaccca attcagcagg aatttatgaa agccaagcta
     3421 ttgttcctac cgcagataga ttctttttcg tgggtttctt ctggacgata agcaaggata
     3481 aaaagacaaa tcagttggag aatttataat ccattcaatt taaatacaat tcatccccaa
     3541 atttcaacag ttcagtcaaa gtaaatttca aaagccttat aacccacata aatttgttca
     3601 agaaataaat aaccaattaa aatttagaaa ttatgacaaa aattattatc gccatcgtat
     3661 tttttattac cagtttagtg tcggctcaag ggcagtttga gcaaggaatg ggaaaagcgt
     3721 tccaactttg gggcgaagga aaaaacaccg aagcatcggc tctattcgaa agaattgcgg
     3781 ctgccgaaaa aacatcttgg ttgcctaatt attatgtggc tttggtaaat acaacaactg
     3841 cttttggcac aaaagacaag gaacaaatca atttgttatt gaccaaagca caaaacgctt
     3901 tagacgttga attaataaaa gcacccaaca atccagaact tttggtgatg caagccatga
     3961 ttcacacggc ttggattgct tatgacccaa tgaccaacgg acaaaaattg tctggaactg
     4021 taatgggatt gtatgccaaa gctgaagcta ttgctccaga aaacccaaga gttgtcttcg
     4081 gaaaagcgga atttgaaatt ggtggagcta aattttgggg aaaagatacc aaaccaatgt
     4141 gtgctcaaat tgaaaaagct attggacttt ttgctacttt taagcctgaa actccatttt
     4201 ctccaaaatg gggattggaa agagcggaag cagctctgaa aaattgtaaa taatttagtg
     4261 attgaaatta aagaattctg ataatgaaaa acgattacgt taaagaatta ccaaaagcat
     4321 tttttattgg cttgactatt tttggagtac taaatcttat tcgatttttt aatggaaata
     4381 gcattgctct tgacgataaa ttggttgtaa tgtttttata tacaatgctt tatagttttg
     4441 cattgcattt ggctaatact tatttgtttt tgtttttgga taaaattttt agcaacgaac
     4501 gtttttcgaa aagaagaata atcatcggtt ttgtttcttc tttttttctg tcgctatttg
     4561 ttatttttct gctgagactt tttattaata ctattattga aaataagtca tttagtgttt
     4621 ttattgccga cgaaagtgct tccgattata ttgtggcttc gatttttact tttgtggtgt
     4681 tgctgatagt ccattttgtt tatctctaca aagggtatca agagaacaaa gtcaaagaac
     4741 aaaaaatcat tgcaggaacg gcaaatgcta aatttgaaag tctgaaaaac caaatcgatc
     4801 cgcattttct atttaatagt ttgaatgttt tgagttcctt gattgaagaa aatcccgaga
     4861 atgcgcagcg atttacgact tctttgtcca agatttaccg ttatgttttg gagcaaaaag
     4921 ataaggaatt ggtttctgta gaagaagaat tagctttcgc taagacctat atgaatttat
     4981 tgaaaatgcg cttcgagaat agcttgtttt atgaattgcc aacatcaatt cttaaccctg
     5041 aagccaaggt cgttccgctc tcgttacagc ttttgttgga aaacaccgta aaacataatg
     5101 tagtttctga acaaaaaccg ttgcacatac gaatttatat tgagggcaat tatttagcca
     5161 ttcaaaatga ttatcaaaaa aaagaagttt tgcaagaacg taaaggtgtt ggcttgcaaa
     5221 atatcatcaa tcgttacgga attattacca acagaaaagt tttgattgaa caaaacgaac
     5281 aaacctttac agtgaaaata cccattttaa ccaaacaaat ttctattatg gaaacagcaa
     5341 cttatagcga caacacggct tattacagag caaaaaaacg agtagaagaa ctcaaaggat
     5401 tttacggaaa tttaatttct tactgctgtg ttataccagt tttaattttt ataaatttaa
     5461 cttatatgcc agaatttcaa tggttttggt tttcagcagc aggttgggga tttggattaa
     5521 caatgcatgc tttcaaagtt tttggataca gttccaattg ggaagaacgt aaaattcaag
     5581 agattttaag gaaagaggaa aataaacaaa cttggaaata atttaatttt tgaaaagatg
     5641 gaaaataagt atcaagaaga agaacgctat tacgaagcca aaaaaagagt agaagaaatc
     5701 aaaggatttt acgggaattt aatttcatat attgttgtaa acattggtct tatggttatt
     5761 aatctgctaa cctcgccaaa atatttatgg tttttttggc caatgttggg ttggggaatt
     5821 ggagtatttt ttcacggaat gaaagtcttt aatttcatgc cttttcttgg aaaagactgg
     5881 gaagagcaaa aaataaaaga gtttatggat aaggaaaaag aaaataaaga aatttggaaa
     5941 taattcaaaa ataaaagaaa tgagacgatc aagaagatat atagacgaat tagaatcgga
     6001 aaattataat ccagacgata gatacagttt ggcgtataag cgtgtaaaaa gaataaaagg
     6061 gttctacgtt catgcaatgg tgtatgtgtt aataaatata tttatagttt atggtaatta
     6121 ctttggaaat tcccataaag attatgattt ttggagttgg caaacctttt caactgcatt
     6181 attttggggc attggtttat tggcacacgg aatgtccgtt tttggaagaa atcttttttt
     6241 tggacaaaac tgggaagaaa gaaagatcca agagtttatg gaaaaagaga aaagcgaaaa
     6301 atgggaatag gaggagtatt cagtagtcag ttttcaggtg ttcagattgc agattttagt
     6361 atacggatta ttcacttcaa aactcttaaa cttcaatctc ccaactccca acttctaacc
     6421 tttaacccct aacttcccaa tgactaccat aataatcgaa gacgaaaaac cggcagcaag
     6481 attgcttcaa cgcaaacttg aaaaactcaa tattgaagta ggagtaatgc ttcattctgt
     6541 agaagaatct atcgaatggt tttctaaaaa tgaacatccc gatttgatct ttttggatat
     6601 ccaattatcg gatggtttgt ctttcgaaat tttcgaaaaa gtggacatca aaagtgccat
     6661 aatttttact acagcttatg atgaatatgc gttaaaagca ttcaaattaa actcgattga
     6721 ttacctctta aaacccatcg acgaagacga tttggaagtg gcaatttcta aatttaaaat
     6781 acgtttgccc aagccggaaa atactcttca attggatttc gaacaaatca aaaaaatgtt
     6841 tcaaaatcca ttcgacaaaa atttcaaaac gcggttcacg gtcaaaatag ggcagcatct
     6901 caaagtgatt tctactgatg aaatcgaatg tttttttagc gaaaataaag gaacttatct
     6961 tcacactttc gacaacagaa attatttgat tgaatctact ttggaagttt tagaacagga
     7021 attagatccc aaaaagtttt atagaattag ccgaaaattt atagttccat taaagtcaat
     7081 taaggaaatt gtggtctata gcaactctcg tttaaaagta attttgccta cttataaaga
     7141 agatgaagtg gttgtgagtc gcgaaaaagt ggcagatttc aaggcatgga ttgggtgatt
     7201 ttttatttaa attttaaaat gatgtaatcg tatataaata tactggtgtt taatgtttta
     7261 aatgcttgat atagatttaa tctattggtg tttttaagcg ttaattgttg ttttgatggg
     7321 gcgaaaacta aatttatttc tgaaagaatc gaaagatatt cagcttctat aaacgactgt
     7381 attgattact tttattttta caggaattgc ttcttttgtt cttgcaaaat ttaaatcacg
     7441 tgtagggatt ttgaaaagta ttggccaatc tcaaattgaa aaacggaatt gtaaagattg
     7501 atttttttaa tgcaacaact gataattttt tgttaatcgt tcccgtgaaa ttagattaag
     7561 attgtacctt cgtttgattg gtaattctta attttttttg aatgaaatat gtactttgtt
     7621 ttagtttttt gtttttactt tcgaatacaa ttcaagccca aaacgtagct gtttttgata
     7681 attattcgag cttgaaaaag gaaattttga gtgataaaag caccat
//
LOCUS       DAOSNN010000113         7717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9445_length_7717_cov_5.767685, whole genome shotgun sequence.
ACCESSION   DAOSNN010000113 DAOSNN010000000
VERSION     DAOSNN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9445_length_7717_cov_5.767685"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLQ70_06990"
     CDS             <1..979
                     /locus_tag="PLQ70_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HQE34400.1"
                     /translation="DWWCSGFYPGTLIYLDEATKKSVFEKEIDRVFQDLQKEQFNKTT
                     HDLGFMMFCSFGNANRLNPTPEYATILMNSAKSLASRFDGKVGCIKSWDAKDDSYLVI
                     IDNMMNLELLFWASEYSGDKSYYDIAIKHANTTLKNHFRPDHSSYHVINYNSKTGEVK
                     QKKTAQGYADESAWARGQAWGLYGFTVMYRATKDKKYLDQAVAIAEFILNNPNLPKDK
                     VPYWDFNAPNIPDALRDSSAGAVMASALLELNSYVDKEHSKKYLNAAKTILETLTTNE
                     YIAEEGTNGGFLLKHGVGHIPQKSEIDVPLTYGDYYLVEAMLRYKKLGK"
     gene            1291..2766
                     /gene="rpoN"
                     /locus_tag="PLQ70_06995"
     CDS             1291..2766
                     /gene="rpoN"
                     /locus_tag="PLQ70_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQE34401.1"
                     /translation="MLKQFLNLKLSQKLSPQQIQLMKLIQLPTQAFEQRLLEEMNENP
                     ALESGNDEEEIYDKDEFNNDDTNDEFDDYDDKDNEDLSDINIDDYLSSDETPDYKTQA
                     NNYSDDDEERESPLVAPISFHQDLINQLNTFILDDSEREIAEFLVGSIDDMGYIRRST
                     QDLVDDMAFTQGIYTDEKNVERMLHIIHELEPSGVGARDLQECLLLQLKHKTPTEYVA
                     LAIDIIENQFDAFTKKHYDKLLQKYAVSNEQLKTAIHEIEKLNPKPGGSFTGSNKITE
                     HVVPDFSIRIEEDELVLTLNGRNAPSLHVSKDYQEMMQTYKSSRDKSTAQKDAVQFIK
                     QKLDSAKWFIDAIKQRQETLYVTMNAIMHYQQDYFLEGDETRLKPMILKDIADMVDLD
                     ISTISRVANSKYVETPYGTKLIKEFFSEAMKNDQGEDVSTLEIKKILKNTIEEEDKHK
                     PLPDDQLAEILKEKGYPIARRTIAKYREQLDIPVARMRKKM"
     gene            2892..3497
                     /locus_tag="PLQ70_07000"
     CDS             2892..3497
                     /locus_tag="PLQ70_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34402.1"
                     /translation="MKKILPFFSYLFHPLFIPVYAALYYFFINNSYHTHPEKYFALFQ
                     IVLITIALPILTYFILKSTGKVNSIMIPDVSQRKIPLFLQCFMIVLLIQKSITIYHFT
                     ELYFFFLGGLLSTIVALILLFFKTKASLHMVSSSALTIFVLALSLHNQTQNLIWIAFL
                     VLMNGFVASSRLEMKAHTPKELVIGFLTGLLPQLFLLSFWL"
     gene            complement(3492..4193)
                     /locus_tag="PLQ70_07005"
     CDS             complement(3492..4193)
                     /locus_tag="PLQ70_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQE34403.1"
                     /translation="MRLFLCCLLFILVLPVFSQETESLVETPKIKIDSLYREDQFYFS
                     FALNTLQKKPVGLTQNKFSSGLTLGFLRDFPINKKRTIAIAPGIGFAFNNYNQNLKIT
                     ESNQIPVYEIIESNVTYNKNRFSQLLVEVPIEFRWRTSTFESHKFWRWYAGFKTGYLM
                     YDKSIYKDANGKIVVSNNKDFNKFQYGTYFSAGYNSINVYAYYGLNPLFKSAKTNTET
                     IDMNSFIVGIMFYIL"
     gene            4541..6976
                     /locus_tag="PLQ70_07010"
     CDS             4541..6976
                     /locus_tag="PLQ70_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HQE34404.1"
                     /translation="MKYGHFDDDSREYVITNPKTPYPWINYLGNEDFFSLTSNTGGGY
                     TFYKDAKFRRLTRYRYNNVPVDNGGKYFYIKEGDSVWSPGWKPVKTELDRYECRHGMS
                     YTKFKSSKNGVQAEVLQFIPLGFWGEIQKLSLKNNSSEVKKLKLFSFIEWALWNAEDD
                     MTNFQRNFSTGEVEVEDSVLYHKTEFKERRNHYSFYSVNEAIKGFDTDRESFIGLYNG
                     FEAPEVVTDGKPKNTIAHGWSPIASHYIEVELKPGESKDYIFMLGYVEVADDQKWESK
                     SIINKKPAKAMIEKYNTVEKVEAAFNELKIYWDNLLSTINIQSGDEKLDRMVNIWNQY
                     QCMVTFNMSRSASFFESGIGRGMGFRDSNQDLIGFVHQIPERARERIVDIASTQFEDG
                     SCYHQYQPLTKKGNAAIGGDFNDDPLWLILSTTEYIKETGDFSLLDELVPFDNDASKA
                     KSHFEHLKISFYHVVNNLGPHQLPLIGRADWNDCLNLNCFSNDPNESFQTTGNKKGET
                     AESLMIAGLFVVYGKEFVKLCQQIGKETEAIEAETHVNNMIEAVKQHGWDGEWYLRAY
                     DYYGKKIGSNENEEGKIFIESQGWCSMAEIGKEEGLVEKSLNAVKEHLDCEYGIVLNS
                     PAFTKYYIEYGEISTYPAGYKENGGIFCHNNPWIMIGETMIGRGDAAYDYYTKIAPSF
                     LEDISQLHKVEPYVYCQMIAGKEAFKPGEAKNSWLSGTASWNFYAITQYILGIKPDYK
                     GLWINPCIPTKWDGFKMTRKFRGATYNIEVSNPKHISKGVEKIIVNGTTIHSNIIPIL
                     EENKTHEIIVIMG"
     gene            complement(7053..7622)
                     /locus_tag="PLQ70_07015"
     CDS             complement(7053..7622)
                     /locus_tag="PLQ70_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQE34405.1"
                     /translation="MLSRRIFKPISDMFNRARFGILFSRETTNLMMPRIMLLSRIKGL
                     PYYLEFISILNDLALSEDQKSLSILSDEKEDFHNSDRIKKVYDYIQENFSKTITLDEI
                     SKLVNMSPVSFNRFIKQRTGKTFVHYLNDTRISFASRWLLETDLSIGEISFRCGFNNI
                     ANFNRLFKQAKNTTPKEFKKQFIGVKRVL"
BASE COUNT         2739 a         1293 c         1322 g         2363 t
ORIGIN      
        1 cgactggtgg tgtagcggtt tttatcctgg aacactcatt tatttagatg aagccacaaa
       61 aaaatcggtt ttcgaaaaag aaattgaccg cgtttttcaa gatttacaaa aagaacaatt
      121 taacaaaacc acacacgatt tgggttttat gatgttttgt agttttggaa atgccaatcg
      181 cttaaatcca acaccagaat atgctacgat tttgatgaat agcgccaaat cattagcttc
      241 tcgttttgac ggaaaagtgg gttgcatcaa atcttgggac gctaaagatg acagctattt
      301 ggtaatcatc gacaatatga tgaatctgga actgttgttt tgggccagcg aatatagcgg
      361 agacaaaagc tattatgata ttgcaataaa acacgccaat accactctca aaaatcattt
      421 tcgccctgac cacagctcct atcatgtcat aaattacaac agcaaaacag gcgaagtcaa
      481 acaaaaaaag acggctcaag gctatgctga cgaatccgct tgggcaagag ggcaagcttg
      541 gggattgtac ggatttacgg taatgtatcg tgctaccaaa gacaaaaagt atttagatca
      601 agcagtggca atagccgaat ttattttaaa taatcctaat ttacccaaag acaaagttcc
      661 ctattgggat tttaatgcac ccaatattcc cgatgcttta agagattctt cggctggtgc
      721 ggttatggct tcggcattat tagaattgaa ttcttatgtt gacaaagagc attcgaaaaa
      781 atatttgaat gctgcaaaaa cgatacttga aaccttaact acgaacgaat atatagctga
      841 ggaaggcaca aacggaggtt ttttattaaa acatggtgtg ggtcatatcc ctcaaaaatc
      901 agaaattgat gtgccgttaa cctatggcga ttattatttg gttgaagcca tgttaagata
      961 caaaaaacta ggtaaataga ttgctttttt gacacgaatt gcacgaattt aaattattta
     1021 aaattgaatt tcacgaattg aattcgtgca attaaaacaa atttttattc gtgtaaattc
     1081 gtgaaattcg tgtctatctt atttaaaaaa acgaatctgt ttgtctttga aacgggttaa
     1141 aatagtaaat ttgcaaactg attataatca acttattcaa tctgtctcgt ataacttgat
     1201 tagtagtcat ttaaaataaa taattagaga ctttttctat ttaataaaaa aaacgttgcg
     1261 cctttgtggc taaataaatt agtgtctttt atgttaaagc aatttttaaa tctaaaatta
     1321 tctcaaaaat tatctccaca acaaatccag ttgatgaagt taattcaact gcctacccaa
     1381 gcattcgaac aacgtttatt agaagaaatg aacgagaatc cggctttgga atcaggaaat
     1441 gatgaggaag aaatttacga caaagacgaa ttcaacaacg atgatactaa cgacgaattc
     1501 gacgattatg atgacaaaga caacgaagac ctcagtgaca ttaatattga tgactattta
     1561 agcagcgacg aaactccaga ttataaaacc caagccaaca attatagcga tgatgatgaa
     1621 gaacgcgaat cgccattggt ggcacccatt agttttcacc aagatttaat caatcaactg
     1681 aacactttta tactcgatga tagcgaacga gaaattgccg aattcttggt aggaagcatc
     1741 gacgatatgg gttatattcg acgaagcaca caagatttag tcgatgatat ggcgtttaca
     1801 caaggcattt ataccgatga aaaaaacgtc gaaagaatgt tgcatatcat tcacgaactc
     1861 gaaccttcgg gcgttggagc acgggattta caggaatgtt tattgctgca attaaagcac
     1921 aaaaccccaa cagaatacgt agctttggcc attgatatca tcgaaaatca gtttgacgct
     1981 tttaccaaaa aacattacga caaactcttg caaaaatatg ccgtttcgaa tgagcaactc
     2041 aaaactgcta ttcacgaaat agaaaaactg aacccaaaac cgggcggttc tttcacagga
     2101 agcaacaaaa ttaccgaaca tgttgttcct gatttttcta ttcgaataga agaagacgaa
     2161 ttggttttga ccctaaacgg aagaaatgct ccgtcgctgc acgtttctaa agattatcaa
     2221 gagatgatgc agacttataa aagttcgcgc gacaaatcga cagcacaaaa agatgccgtt
     2281 cagttcatca aacaaaaact ggattcggcc aaatggttta ttgacgctat caaacaacgc
     2341 caagaaacac tgtatgtaac gatgaacgcc attatgcatt atcagcaaga ctatttctta
     2401 gaaggcgatg aaaccagact caaacccatg attctaaaag acattgctga catggtagat
     2461 ttggatattt cgaccatttc acgtgttgcc aatagcaaat atgtagaaac gccttatgga
     2521 accaaattga ttaaagaatt cttctcggaa gcaatgaaaa atgaccaagg agaggatgtt
     2581 tcgacccttg aaatcaagaa aattttgaaa aacactatcg aagaagaaga caaacacaaa
     2641 ccacttccag acgatcaact agccgaaatt ctaaaagaaa aaggctatcc aattgctcga
     2701 agaaccatcg ctaaatatag agaacaactg gatattccag tggcaagaat gagaaagaaa
     2761 atgtaggaat tttagattgc agattaacgg tttttgattt cagatttgtt gttgtcagta
     2821 aagactctaa aattaaatga ttttgaaaat gatttaattc ccatttaaat atttaattta
     2881 aacaagcaca cttgaaaaaa atccttcctt ttttttccta tttattccat ccgttattca
     2941 ttccggtata tgcagcctta tattactttt ttatcaacaa ttcctatcat acccatccag
     3001 aaaaatactt tgctcttttt caaattgtct taattacaat tgccttaccc atcctgacgt
     3061 attttatttt aaaatctaca ggaaaagtga attctataat gattcccgat gtttctcagc
     3121 gcaaaatacc attgtttttg caatgcttta tgatcgtttt attgatacaa aaaagcatta
     3181 caatctacca ttttaccgaa ttgtattttt tctttttggg aggattgcta agtacaattg
     3241 tagctttgat tttgttgttt ttcaaaacaa aagctagttt gcatatggta tccagtagtg
     3301 ctttaacgat ttttgtactg gcgctaagtc ttcacaatca aactcagaat ttaatttgga
     3361 ttgcttttct ggtacttatg aatggttttg tagcctcgtc tagactagaa atgaaagcac
     3421 atacgccaaa agaattagtg attggttttt taacaggctt acttccgcaa ctgtttttgt
     3481 tgtctttttg gttatagaat ataaaacatg attccaacaa taaatgaatt catgtctata
     3541 gtttcagtat tagtttttgc cgatttaaac aacggattca aaccataata agcataaaca
     3601 tttatcgaat tgtaccctgc agaaaaataa gttccgtatt gaaatttatt aaagtcttta
     3661 ttgttactca ccacaatttt accattggca tctttgtaaa ttgatttgtc atacattaaa
     3721 tagcctgttt taaatcctgc ataccatcgc caaaatttat ggctttcaaa agtagatgtt
     3781 ctccatctaa actcaatggg cacttcgact aaaagctgac tgaatctatt tttattataa
     3841 gtaacattag attcaattat ttcataaacc ggaatttggt tagactctgt aatcttaaga
     3901 ttctgattgt agttgttgaa tgcaaatcct attcctggtg ctatggcaat ggttcgtttt
     3961 ttattgatag gaaagtctct aagaaaaccc aaagttaatc cagacgaaaa tttattttgt
     4021 gttaaaccta cgggtttctt ctgcaaagta ttaagagcga aactaaaata aaactgatct
     4081 tctctataaa gagagtcaat ttttatttta ggagtttcaa ctaaactttc tgtctcttgc
     4141 gaaaaaacag gaagaactaa tatgaaaagc aaacagcaaa gaaataatcg cataccttat
     4201 tttttaaggg ataaagttat aaaaaaacac aacagcaaaa atttcttttt tttggcaata
     4261 aaaactaatc tcataaatcc agaaaaaact aatcctatcg actgtttaaa actctttatt
     4321 ttctgatacg aaagctagtt tgagttaaaa aaagataggt aatagacaaa atagtataaa
     4381 gaaatattta gttagttggc taattttgaa aaatgctaat tataccacct gtctattatc
     4441 aaattagcaa aaaaacctat ctaccaatac gaattagtta ctattaaccc taaaacacaa
     4501 ctttgattgt gttttgaagt taatttaaaa tataataaaa atgaaatacg gccattttga
     4561 tgatgattct agagaatatg taatcacaaa ccctaaaaca ccttaccctt ggattaatta
     4621 tttggggaat gaagattttt tctccttaac ttcaaatact ggaggtggtt atacttttta
     4681 taaagatgca aaatttagaa gattaactcg ttatagatat aataatgttc ctgttgataa
     4741 tggcggaaaa tatttttaca taaaagaagg tgactccgtt tggtctccag gatggaaacc
     4801 cgtaaaaaca gaactagacc gctacgaatg tcgtcacgga atgagttaca ctaaattcaa
     4861 aagttctaaa aacggagttc aagccgaagt tttacagttt attccactag ggttttgggg
     4921 cgaaattcaa aaattaagct taaaaaataa ttcgtcggaa gtaaaaaaat taaaattatt
     4981 ctccttcatc gaatgggcac tttggaatgc tgaggacgat atgaccaatt ttcaaagaaa
     5041 tttcagtact ggcgaagtgg aagtggaaga ttcggtacta taccacaaaa ccgaattcaa
     5101 agaacgccga aatcattatt ctttttattc tgtaaatgaa gccatcaaag ggtttgacac
     5161 cgatagagaa tcatttattg gattgtacaa tggatttgaa gccccagaag tggtcaccga
     5221 tggaaaacct aaaaatacca tagctcacgg atggtctcct atagcctccc attatatcga
     5281 agtcgaatta aaaccaggag aaagcaaaga ttatattttc atgcttggtt atgttgaagt
     5341 tgccgatgac caaaaatggg aaagcaaatc aatcatcaat aaaaaaccag caaaggcgat
     5401 gattgaaaaa tacaatacag tcgaaaaagt agaagccgca tttaatgaat taaaaatcta
     5461 ttgggacaat ttacttagca caattaatat ccaatcgggt gacgaaaaac tagatagaat
     5521 ggtcaatatc tggaaccaat atcaatgtat ggttaccttt aatatgtcac gttctgcttc
     5581 gtttttcgaa tcgggaattg gtcgcggaat gggctttcga gattccaatc aagatttaat
     5641 tggttttgta catcaaattc ctgaaagagc cagagaacgc attgttgata ttgcttcaac
     5701 acaatttgaa gatggttctt gctaccacca ataccagcct ttgaccaaaa aaggaaatgc
     5761 cgcaatcgga ggcgatttca acgatgaccc gctttggcta atcctttcaa ccactgaata
     5821 catcaaagaa actggcgatt tttcgctact cgacgaatta gttccatttg acaatgatgc
     5881 ttcaaaagca aaaagtcatt tcgaacattt gaaaatttcg ttttaccacg tcgtaaataa
     5941 tttaggacct catcaattgc ccttaatagg acgagccgat tggaacgact gtttgaactt
     6001 gaattgtttt tcaaacgacc cgaacgaatc ttttcaaact actggaaaca aaaaaggtga
     6061 aactgccgaa tcccttatga ttgctggatt gtttgtggtt tatggcaaag agtttgtgaa
     6121 actgtgccaa caaataggaa aagaaaccga agcaattgaa gccgaaactc acgtaaataa
     6181 tatgattgaa gctgtaaaac aacacggctg ggatggcgaa tggtatttga gagcttatga
     6241 ttattatggc aaaaaaatag gttctaacga aaatgaagag ggaaaaatat tcattgagtc
     6301 gcaaggttgg tgttctatgg ccgagattgg caaagaagaa ggcttggtcg agaaatcttt
     6361 gaatgcagta aaagaacatt tagattgcga atacggaatc gtattgaata gtcccgcatt
     6421 tacaaaatat tatatcgaat atggcgaaat ttcaacttat ccagcgggtt acaaagaaaa
     6481 cggcggtatc ttttgtcaca acaacccttg gattatgatt ggcgaaacga tgataggacg
     6541 cggtgatgca gcttatgatt attacaccaa aattgctcca agttttttag aagacatttc
     6601 tcaattgcac aaagtagagc cttatgttta ttgccaaatg attgctggta aagaagcttt
     6661 taaacccgga gaagctaaaa attcttggtt atcaggaaca gcatcttgga acttttatgc
     6721 cataacacaa tatattttgg gtataaaacc agattacaaa ggactttgga taaatccttg
     6781 tattccaact aaatgggatg gtttcaaaat gactcgaaaa tttagaggag caacatacaa
     6841 tattgaggtg tcaaacccaa aacatattag taaaggtgtc gaaaaaataa tagtaaatgg
     6901 aactacaata cactcgaata tcattccaat attagaagaa aataaaaccc atgaaataat
     6961 tgtaattatg ggctaggcat ttgttagtta gtaatgaaaa actctcttag aattttagtc
     7021 ttctttgaga gtttttctat tataatgagg agttacaaaa ctctttttac ccctataaat
     7081 tgttttttga attcctttgg agttgtattt ttagcttgtt taaacaatct attaaaattg
     7141 gcaatattat taaatccgca tctaaaacta atttctccaa tactcaaatc agtctctaac
     7201 aaccatctcg aggcaaaact aattcgggtg tcgtttaaat aatgaacaaa tgtttttcct
     7261 gttctttgtt tgataaatct attaaatgaa acaggactca tgttgaccaa ctttgaaatc
     7321 tcatctaatg tgatagtttt cgaaaaattt tcttgaatat aatcgtacac ttttttaatt
     7381 cgatcactat tatgaaaatc ttctttttca tcacttaaaa tagaaagcga tttttgatct
     7441 tcagaaagcg caaggtcatt cagaatagaa ataaactcta aataatatgg caaaccctta
     7501 attcttgata atagcataat tctaggcatc atcaaatttg tggtttcacg agaaaaaaga
     7561 ataccaaatc ttgctctatt aaacatatcg ctgattggct tgaaaattct tctcgacaac
     7621 atttttttat ctagcaaatc attgtgaata tgtatcgtga ttttggatat gcaaataaaa
     7681 actacacatt aagttaagct acatttttgt aattaat
//
LOCUS       DAOSNN010000114         7700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9479_length_7700_cov_5.076651, whole genome shotgun sequence.
ACCESSION   DAOSNN010000114 DAOSNN010000000
VERSION     DAOSNN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9479_length_7700_cov_5.076651"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1404
                     /locus_tag="PLQ70_07020"
     CDS             <1..>1404
                     /locus_tag="PLQ70_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQE34406.1"
                     /translation="IIGVKDKTFFTPKAYAAWSKLMNSLKEDKAVALVISINDLKKLQ
                     KNEALEKFELVPFVDDTKTTDKTYLEKIKKELFNDLPFYEGLLFNKRSGTIRSAIYLD
                     KKIVNTPARKDFILKKLVPAIEAFEKETNIDLRVSGMPYIRTLNAQTIISEISIFIGA
                     SLLVTSLLFFFFFRSFRATLISIIIVIIGVMWSFGILGLLNYEITVLTALVPSLIIVI
                     GIPNCIFLTNKYHQEYKIHRNKVKALQRVTTKVGMATLLTNVTTAIGFFTFISSNNKL
                     LIEFGNATSINILALFFLCIVVIPIFHSYIPAPKDRHIEHLDRGYVKSFMDWILRNVK
                     ENRFSIYVVAVSLLVISIIGIYKMRISGSIIEDMPKKAPFFQDIIFFEKEFDGVMPLE
                     IMIDTKHKKGVMKLSTLKRMDELEQAITEIQGLSKPISIVNLVKYSKQAYYNGNPEYY
                     ELPTSQEQAFILSYAKNA"
     assembly_gap    1405..1504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1505..1876
                     /locus_tag="PLQ70_07025"
     CDS             <1505..1876
                     /locus_tag="PLQ70_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HQE34407.1"
                     /translation="FGLSVDDTVRFLAQYREELKKNNWKIRKSVYATFNDAGLSMFYT
                     SIVLFFGFSVFMLSSFGGTIALGGLISLTLLFGMLSNLMLLPALVLTLNKTLANEQEF
                     IEPMIDIIEASDEEIDALENN"
     gene            2149..3588
                     /gene="asnS"
                     /locus_tag="PLQ70_07030"
     CDS             2149..3588
                     /gene="asnS"
                     /locus_tag="PLQ70_07030"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HQE34408.1"
                     /translation="MKLRHTRVKDLLNSTNTLHEVNAKGWVRTFRNNQFIALNDGSTI
                     NNIQCVVDFENTAEETLKRITTGAAVSVTGDLVESKGAGQKFEIKVRKLEILGDSDAE
                     KFPMQPKKHSLEFLRENAHLRVRTNAFGAIMRVRSVLSFAVHNYFQQKGFVYVNTPII
                     TGADAEGAGEMFQVTSLPLDNLPKNEEGKIDYKKDFFGQHTNLTVSGQLEGETFAMAL
                     GQIYTFGPTFRAENSNTSRHLAEFWMIEPEVAFNNLDDNMDLAEDFIQYVIKYAMDKC
                     GDDLKFLEGRLLEEEKSKPQAERSEMALLEKLNFVLENNFKRVSYTEAIDILRDSTPN
                     KKKKFQYLINEWGADLQSEHERYLVEKHFKSPVILFDYPANIKAFYMRLNEDGKTVRA
                     MDILFPGIGEIVGGSQREERFDVLVEKMKALGIDEEELWWYLDTRRFGSAVHSGFGLG
                     FERLVLFVTGMTNIRDVIPFPRTPQNAAF"
     gene            complement(3691..>5621)
                     /locus_tag="PLQ70_07035"
     CDS             complement(3691..>5621)
                     /locus_tag="PLQ70_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HQE34409.1"
                     /translation="IVNPNYELSPYTGMTKQHWKDAAFYLLKGAFSYIHNLDDPMKFP
                     KQPGVSYPIDEVKVPTEKLEGLCRTLFIAAPLLKDNPNLVINNIKVADYYRYQMGLLT
                     NPASPSYIVPRPKNGGPNQNLVEFGALSVSMLIAPEILWDPLPQVQKDELAQTMLSYG
                     DGPTVPSNWKFFNIFVMSFFKSKGYAVNEPLMVEYLQKSLDHYRGDGWYNDNPAFDYY
                     SMWAFQMYGMLWSEYIGKQYYPEFAAKFEDNFKDVKNNYPYMFSRNGEMIMWGRSISY
                     RIGATIPFPLMGFETDKAAVNFGWMRRISSGVLQQFLQNPNFMKDNVPTLGFYGAFEP
                     AVQSYSCRGSVYWMGKAFLGLMVPDDNPFWTAKENEGPWGKEFEKNTVYNKFQKGSEI
                     LITDYSNIGAAELRAWCNVPVIKAWEKFRGSENYNRLSYNSAFPWQADGANGEVAMNY
                     VFKNSKKEWEPFRMYTFKKFEEGIYYRDVVLETNPNVKFNLADIPLANGILRVDKNNS
                     SDSIEMRLGHYALPKLDIEIKTTVKKVKGHQITIIDNGKYQLAMIPLLGWDKMEVVKS
                     KGLYPAADESAVINLSHNFVPQKDHSEIFATLMLWKKSGEKWTDKELLPVKTIQYSKT
                     TNTAKISIRKGGNREVKF"
     assembly_gap    5622..5721
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6204..7517
                     /locus_tag="PLQ70_07040"
     CDS             6204..7517
                     /locus_tag="PLQ70_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013070408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HQE34410.1"
                     /translation="MKNKKIIKGFICGLLLFLTACTSVKTVQTDIGYGWNNNSVNVVK
                     FRKNALTTFQNYQFTAYYDENSYLILAKRKVNSSDWEKVKTPYKGNTKDAHNDISIAI
                     DGDGFLHISWDHHDTKLRYAKGKSPMSLDLGEEQSMTGKEELKVTYPQFYNLPNGNLL
                     FFYRSGASGRGNMVINFYDVKSKTWSQLQQNLVNGEEKRSAYWQSSIDQQGVIHLSWV
                     WRESWDVSTNHDLCYARSKDGGITWEKSSGENYKLPITASTAEIAWKIPQKSSLINQT
                     SMATDENGNPYIATYWSENKIPQYQIIYLRNGNWKKISTQFRKTPFYLGGGGTKQIPI
                     SRPDILIANKGNNSKLYLLFRDEERGNKISLAYIQFNKESNWKIVDLTKDSVGQWEPN
                     YDNSLWEKENKLHIFMQNVTQIDGEGLAKIAPSNVSVIEVNHLPK"
BASE COUNT         2497 a         1328 c         1327 g         2348 t
ORIGIN      
        1 cataattggc gtaaaagaca aaactttttt taccccaaag gcttatgccg cttggtctaa
       61 attaatgaac agcttgaaag aggacaaggc tgttgcttta gtaatttcta taaacgattt
      121 aaaaaaatta caaaaaaacg aggcgctcga aaaatttgaa ttggttcctt ttgtagatga
      181 taccaaaact actgacaaga cctatcttga gaaaataaaa aaagagctct ttaatgattt
      241 gcctttctat gaaggattgc ttttcaataa aagatcagga acgattagat ctgctatata
      301 tcttgataaa aaaattgtaa atacacctgc ccgaaaagat tttattctaa aaaaattagt
      361 tccagcaatt gaagctttcg aaaaagaaac caacatcgat ttgcgtgttt ctggaatgcc
      421 ctacatccga acgcttaatg cacaaaccat tattagtgaa attagcatct tcattggtgc
      481 atcattatta gtaacatctt tacttttctt tttctttttt agaagtttta gagctacctt
      541 gatttctata attattgtga ttattggtgt aatgtggtct tttggcatct tgggattatt
      601 gaattacgaa atcacagttt taaccgcatt ggttccttct ttaatcattg taatcgggat
      661 tccaaattgt attttcctca ccaataaata ccaccaggaa tataaaattc atcgaaataa
      721 agttaaggca ctacaaaggg ttactaccaa agtgggtatg gcaacacttt tgaccaacgt
      781 aaccacagct attggttttt ttacttttat ctcatcaaac aataagctgc ttattgagtt
      841 tggaaatgct acttccatta atattttagc tttatttttc ttatgtatag ttgttattcc
      901 tatttttcac agttacattc cagcccctaa agaccgtcac atcgagcatt tagacagagg
      961 ttatgtaaaa tcatttatgg attggatact acgcaatgtt aaagaaaatc gattttctat
     1021 ttatgtagtt gctgtttcac ttctggtaat cagtataatt ggaatttaca aaatgcgtat
     1081 ttcgggaagc atcatagaag atatgcctaa aaaagccccc tttttccaag acattatttt
     1141 cttcgaaaaa gagtttgatg gcgtgatgcc tttggaaatt atgattgaca ccaaacacaa
     1201 aaaaggagtc atgaaacttt ctacgctcaa gcggatggac gaactagaac aagcaattac
     1261 cgaaattcaa ggattatcaa aacccatttc gattgtcaat ttagtgaaat attctaaaca
     1321 agcttactat aacggaaatc ctgaatatta cgaattgcca acctcccaag agcaagcttt
     1381 catcttgtcg tatgccaaaa atgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnntttgga ctttcggtcg atgataccgt tcgattcttg gcgcaatatc gagaggaatt
     1561 aaagaaaaac aactggaaaa tacgcaaatc agtttacgcc acttttaacg atgctggttt
     1621 gagtatgttc tatacttcta tcgtgttgtt cttcggattt tcggtattta tgttgtctag
     1681 ttttggcgga accattgccc ttggcgggtt gatttcattg actttattat tcggaatgct
     1741 atctaattta atgctattgc ctgccttggt attaacttta aacaaaacat tggccaacga
     1801 gcaagaattc atcgaaccaa tgattgacat catcgaagct agcgatgaag aaattgacgc
     1861 tttagagaat aactaaatta ttttaagaaa ggttttaaga aattttcaaa ctatttagga
     1921 ataaaaaagc acctgaaatc caatttaatg gtttttgggt gctttttatc attttaatta
     1981 gaaattcgcc taggaaaaat agctctccat ttaactttat aaaaagtgag atacaaaatt
     2041 tagaagcaaa tagcttttga atagccattt caaacaatgc tatttgaaac aaatcgatta
     2101 tatttacaca tcgattgaag aattgaaaaa ttaaatacga aatctacaat gaaattaaga
     2161 catacaagag ttaaagactt actaaacagc accaataccc ttcacgaagt caatgcaaaa
     2221 ggatgggtaa gaacctttag aaacaaccag tttattgctt tgaacgacgg ttcgaccatc
     2281 aataatatac aatgtgttgt cgattttgaa aacacagccg aagaaacttt gaaaagaatt
     2341 actactggag cggcggtttc ggttacggga gacttggtcg aaagcaaagg tgctggacag
     2401 aaatttgaaa ttaaggtacg aaaactagaa attcttggcg attcggatgc cgagaaattc
     2461 ccgatgcagc ctaagaaaca ttccctagaa ttcttgcgtg aaaatgcgca tttaagagtt
     2521 cgtaccaatg cttttggagc cataatgcga gtgcgttcgg ttttgtcttt tgcggttcac
     2581 aattattttc agcaaaaagg gtttgtgtat gtaaacacgc ccatcattac gggagctgat
     2641 gcagaaggtg ctggcgaaat gtttcaagta acttcgttgc cattagataa tttgcctaaa
     2701 aacgaagaag gcaaaattga ttataaaaaa gatttcttcg gacaacacac caacttaacc
     2761 gtttctggac aattagaagg cgaaactttt gctatggctt tgggtcagat ttatactttt
     2821 ggtcccactt ttagagccga aaactcgaat acatcgcgcc acttggcgga attttggatg
     2881 attgaacccg aagtggcttt caataatttg gatgataata tggatttggc agaagatttt
     2941 atccaatatg taattaaata tgcaatggat aaatgtggcg atgatttgaa atttctggaa
     3001 ggaagacttt tagaagaaga aaaatcaaaa ccacaagccg aaagaagcga aatggcttta
     3061 ctagaaaaat taaactttgt gttggaaaac aatttcaaac gcgtttctta caccgaagcg
     3121 attgatattt taagagattc aactcccaat aaaaagaaaa aatttcaata cctcatcaac
     3181 gaatggggtg ctgatttaca atcggaacat gagcgttatt tggtggaaaa acactttaaa
     3241 tcacctgtga ttttatttga ttatccagcg aatatcaaag cattttatat gcgtttgaac
     3301 gaagatggaa aaaccgtccg tgcgatggat atccttttcc ctggaattgg agaaattgta
     3361 ggcggttcac aaagagaaga gcgttttgac gttttggtcg aaaaaatgaa agccttagga
     3421 attgacgaag aagaattgtg gtggtattta gacactcgca gatttggttc ggcagttcat
     3481 tctggttttg gattgggctt cgaaagatta gtgttgtttg ttactggaat gactaacatt
     3541 cgtgacgtaa ttccttttcc aagaacacca caaaatgcag ctttttaaaa aactagttta
     3601 aaggtttgaa attgtttaag agtttaatgt tgttaaaact tccactacat taaactctta
     3661 aacctttaaa caccttttaa ccctttactg tcaaaatttt acttctctat taccaccttt
     3721 tcgaatacta atttttgcag tatttgtagt ttttgaatat tggattgttt ttacgggcaa
     3781 caattctttg tcggtccatt tttcgcccga ctttttccac aacattaagg tagcaaaaat
     3841 ttcagaatgg tctttttgag gcacaaaatt atgactcaag ttgattacag cgctttcgtc
     3901 ggcagcagga tacaaacctt ttgattttac gacttccatt ttatcccaac ccaaaagcgg
     3961 aatcattgcc aattggtatt ttccgttgtc gataatcgtt atttgatgtc cttttacttt
     4021 tttgacagta gttttaattt ctatatctaa tttgggcaaa gcataatgtc ctaatcgcat
     4081 ttcgattgaa tctgaactat tgtttttatc tactcttaaa atgccattcg ccaaaggaat
     4141 atcggccaaa ttaaatttaa cattaggatt ggtttccaaa acgacatcac ggtaataaat
     4201 tccttcttca aactttttaa aagtgtacat tctgaaaggc tcccattctt ttttactgtt
     4261 tttgaaaaca taattcattg ccacttcccc atttgcgcca tcggcttgcc aaggaaacgc
     4321 actgttataa gaaagtcggt tgtagttttc gcttccacga aatttttccc aagctttgat
     4381 tacaggaaca ttgcaccaag ctctcaattc agctgcacca atattagaat aatcggtgat
     4441 taaaatttct gatccttttt ggaatttatt gtaaacggtg tttttttcaa attcctttcc
     4501 ccaaggacct tcattttctt ttgcagtcca aaacggatta tcatcgggaa ccatcaaacc
     4561 cagaaatgct ttccccatcc aataaacact tcctcgacaa ctgtaacttt gcacggcagg
     4621 ttcaaaagca ccataaaatc ctaaagttgg cacattgtct ttcataaaat tggggttttg
     4681 caaaaactgt tgcaacacac cagacgatat tcttcgcatc catccaaaat tcacggctgc
     4741 tttatcggtt tcaaatccca tcaatggaaa cggaatcgta gctccaatgc gataactgat
     4801 gctacgtccc cacataatca tttcgccatt tcgactaaac atataaggat aattattttt
     4861 tacatcttta aaattatcct cgaatttggc agcaaactca ggataatatt gctttccgat
     4921 atattctgac cacagcattc catacatttg aaaagcccac atactataat aatcaaaagc
     4981 aggattgtca ttataccatc catcgccacg ataatggtct aatgattttt gtaaatattc
     5041 taccatcaaa ggttcgttga cagcatagcc ttttgatttg aaaaaactca ttacaaaaat
     5101 gttgaaaaac ttccagttgg agggaactgt tggaccatcg ccataactta acatcgtttg
     5161 ggcaagttca tctttttgaa cttgcggcag tggatcccaa agtatttctg gagcaatcaa
     5221 catcgaaaca gacaacgcac caaattcgac caaattctgg ttgggacctc catttttagg
     5281 acgaggaaca atataagatg gactcgctgg attggtcaat aaacccattt gatagcggta
     5341 ataatcggca acttttatgt tattgataac taagttggga ttgtccttta ataacggagc
     5401 cgcaataaat agcgttctac aaagtccttc gagtttttcg gttggtactt tcacttcatc
     5461 tattggataa ctaactccgg gttgtttggg gaatttcatc ggatcatcca gattgtgaat
     5521 ataggaaaaa gcacctttca gcaaataaaa agccgcgtct ttccaatgct gcttggtcat
     5581 tccggtatat ggacttaact cgtaattggg gtttacgatt tnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn ntgctgcttg gtcattccgg tatatggact taactcgtaa
     5761 ttggggttta cgattttaaa aacattgtct tctgttggat tcataaccgt ttttttttct
     5821 tgtgccacta tggacaaatt catcagaagc aacaaaataa acgaatatat ttttttcata
     5881 ataagctttt agattccatt acattatttg caaaccaaag ttacttaata atctgatgaa
     5941 aaaaaatcat ccctgttatg ccaatgataa ttttcaattg cctccaactt tagttgaagg
     6001 aattaggaat aagtaaattg gctttagcca aaaatctgat attttttggg ctaaagccat
     6061 taaaaatcta caaatttacc ccccccaact aaagttggag gcaattgaca ctttgaaggc
     6121 aaacaggcta aatagtaaca gaaaatcaac ttataattgg tatatttgaa acaaaaaatt
     6181 taagtttttc caagtagaaa ttaatgaaaa acaaaaaaat aatcaaagga tttatttgcg
     6241 gtttgctgct gtttttaacg gcttgcactt ctgtaaaaac ggttcaaaca gacattggtt
     6301 acggctggaa caacaattcg gtgaacgttg taaaatttcg aaaaaatgct ttaaccactt
     6361 ttcaaaacta tcaatttaca gcctattatg atgagaatag ttatcttatt ctcgccaaac
     6421 gaaaagtaaa ttcttccgac tgggaaaaag taaaaactcc gtataaagga aacacaaaag
     6481 acgcacataa cgacattagc atcgccattg atggcgatgg ttttttgcac attagttggg
     6541 atcatcacga caccaaactt cggtatgcca aaggaaaaag cccaatgagt ttagaccttg
     6601 gcgaagaaca atcgatgacg ggcaaagaag aactaaaagt tacttatcct cagttttata
     6661 atttacccaa cgggaatttg ctcttttttt atcgttcagg agcttcgggc agaggcaata
     6721 tggtcatcaa tttttatgat gtaaaaagca aaacttggtc gcagttgcaa cagaatttag
     6781 taaacggcga agaaaaaaga agtgcctatt ggcaatcgag tatcgaccaa caaggcgtta
     6841 ttcatctatc gtgggtgtgg cgcgaaagtt gggatgtttc caccaatcat gatctgtgtt
     6901 atgctcgttc caaagatggt ggaatcactt gggaaaagtc ttcaggcgaa aattacaaac
     6961 tccccattac ggcatcaaca gccgaaattg cttggaaaat tcctcaaaaa agcagcctta
     7021 ttaatcaaac ttcgatggca actgacgaaa atggaaatcc ttatattgca acctattgga
     7081 gtgaaaataa aattccgcaa tatcaaatca tttatttaag aaacgggaat tggaaaaaaa
     7141 taagtactca attcaggaaa actccttttt atctaggagg aggaggaact aagcaaattc
     7201 cgatttcgag acccgatata ttgattgcta acaaaggtaa taattcaaaa ttataccttc
     7261 tatttagaga tgaagaaaga ggcaacaaaa tttcgctggc ttatatccaa tttaacaaag
     7321 aatctaactg gaaaattgtt gatttgacta aagattccgt tggacaatgg gaacctaatt
     7381 acgacaactc tttatgggaa aaagaaaata aattacatat ttttatgcaa aatgtcaccc
     7441 aaattgacgg cgaaggatta gccaaaatcg ccccaagtaa tgtaagtgtc atagaagtaa
     7501 atcatttgcc taaataattt aaaaacaaac ttaaatgaaa ccatttattg aatcgaatat
     7561 tttgagaaat ttaaaaagaa ccctaattgt tggagcagga atagcattgc tttttagctc
     7621 gtgtgcggga aataaattag acaagcagat tgattttgcc ctgagtcgtg gcgtaaatca
     7681 atacagttat atgatgaaaa
//
LOCUS       DAOSNN010000115         7688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9509_length_7688_cov_5.729857, whole genome shotgun sequence.
ACCESSION   DAOSNN010000115 DAOSNN010000000
VERSION     DAOSNN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9509_length_7688_cov_5.729857"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLQ70_07045"
     CDS             complement(<1..724)
                     /locus_tag="PLQ70_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34411.1"
                     /translation="MKKNLLLTAFLFSIAMIGQQKIDRKALVTRHNVKNTAIDTLASL
                     SVGNGAFAFTVDATGLQTFPEKYQKGVPLGTQSEWGWDSYKNTNNYKFSETLRDYDQY
                     GRKISYSVQEKSTPRKNEAVNWFRQNAHLLQLGNLGFEITKKDGTLAKAEDIESIDQT
                     LNLWTGEIESSFKVEGVWVKVKTLSHQEKDAIGVKVESDLVQQGRLKIRLRIPYPTGL
                     WGDMGTKWEEKQDVTISAVAIAG"
     gene            complement(825..4472)
                     /locus_tag="PLQ70_07050"
     CDS             complement(825..4472)
                     /locus_tag="PLQ70_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008612671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HQE34412.1"
                     /translation="MQLFKIKLKLTFFWIFVLLLSFPLASLSQEKNLPSERKIHYAPE
                     GNSFVLKNGSRKFNRALYGTNTAFRVETGDLPEFALYLPGMGGNFKLGIQKGDQSKWI
                     TEATKIDARYTLGMMYYKIQDPILGNGELLLEVIALKNTEGFGLKVTGNNISNDLSLI
                     WAFGGASGKKFSRDGDIGADPESVFYMLPNYCTNNKYTVQKESFVLEYGSEVNKEKTG
                     NAKSLIGFYPNSEIHLANANQQKSPLELFGSAPESLTVITGKIATISKNPLFWMIETD
                     AKTQLKNQKELATIFENSVQETQLLANRIKVVTPNPYINTLGSALAIAADGIWESPAY
                     LHGAIAWRMYLNAWRGAYTADPLGWHDRAKSHFTSYSKSQVTEPLSGPVTPDVSRNLA
                     RETEKMGTTLFTSGYISRRPNNNTIAHHYDMNLVFFDQMLRHFKWTGDVTFMKEMWPV
                     IQRHLAWEKRNFDTNNDGLYDAYAAIWASDALQYSGGAVTHSSAYNYYSNKEVAAVAK
                     YLKQDNSVFLNESKKIFDATNSQLWMPKKGIFAEYKDALGNQLVHDSPGVWSIYHTID
                     SELSNPFQSYQMLRYLDAKIPHIPMTAEGLDKKDLYAISTTNWQPYDWSLNNVALAEQ
                     LHTSLAFWQGGQSEKAFKMWESALIGSMYLGASPGGFEQLSFYDAMRGELYRDFADPI
                     GMTSRTLVEGLFGIQPNALENRLTIQPGFPVEWNSASIEIPDASIAFTREKNTDSYEI
                     KTHFTTKMDLKFVAKIPYENIKSVTINGEKATWKTISDAVGSPQLIVESPYNESYKVS
                     ITWEGEKLEEPQLDKVYCIGESIKLQTKKAKIIDIYDPQTALSEISKTNSELKANLKT
                     KEDATFFVKLQQGNTTWWSPINLIVKPEIEVKSTRIENNNLLFAIKNNSQKAIDGNLI
                     FTPFGKEISQKVSLNKDSETTISIPFENLISGTNAVELKTNEGKTTSLSFTNWDIVIP
                     KETKLETISLSDKFNSKVTDIFKQEYLSPRPTSPTLQLPKHGIGNWCYPNLDVKIDDS
                     GLRQNAKNSNQITSPEGILFQTPADANSKNIAFTSMWDIFPDSIAVPLSGKASHLYLM
                     MAGTTNPMQSRFVNGEITVNYTDGSSEVLQLKNPQNWWPIEQDYFTNGLAFTTDAPKP
                     PRVYLKSGTISRTFKDFSSIKGFSNYAVDGGAATILDLPLNKNKTLKNITLKSIANDV
                     VIGLMSATLVR"
     gene            4690..6027
                     /locus_tag="PLQ70_07055"
     CDS             4690..6027
                     /locus_tag="PLQ70_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HQE34413.1"
                     /translation="MNKIYPLLALIVCFFLPFKSLASETNSSSQMMNGSKSQTFHFGT
                     KKSSSKVIGISNSLVYNSNTGYGFDFETAANVKMDSNTFSIEKPTYFSVKLPEGNYKV
                     EVTIGSKEKASNVTIKAESRRLMLNQFSVKKGSQITKTFNVNLRFPQIDATSKISLKD
                     RDLSVFNWDDKLTLEFLGVANIQSIKITPVTTIPVIYLAGDSTVTDQDVEPWASWGQF
                     ITNYFDDKVVVANYAVSGSALSSFKASNRLKKINSLIKPGDYLFIEFAHNDEKLKGPE
                     NTAWESYSKYLEEYVEMARSKGAFPILVTPTQRRAFNPDGTLKPTHGDFPDAMRAVAK
                     KMNVPLIDVTQMTTQLYEKWGDGPSRKAFVQYPANTFPGQLKALEDNTHFNSFGANEI
                     ALCVIKGIRELGLPLKNNITKETPAYDPTKPNSFSNWTLPMSARFEIVKPDGN"
     gene            6050..>7688
                     /locus_tag="PLQ70_07060"
     CDS             6050..>7688
                     /locus_tag="PLQ70_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQE34414.1"
                     /translation="MLLKKNLQFIAIAALTTFSALHSFAQQTDKVFLSGKDFEHPVQW
                     DFYCTDGNNSKAWSKINVPSQWELQGFGTYTYGRWYKELNEKEPSKEEGLYKYEFEVP
                     ADYKEKDILIAFGGAMTDTEVKVNGKLAGEIHQGGFYEFKYDITPLLNFGSKNILEVH
                     VWKHSANPSVNAAERRADWWLFGGIYRPVWLEVSPKTQIQHIAVNPKMDGSITVDMNL
                     KNIAKNTIVEATLKGLDGEKFDTFSFPIKVKSTKETIAAQWKNVKPWNTETPNLYNLE
                     LVLKQNGTLIHKVEKRIGFRTLEFKKKDGIYVNGTKIVMKGINRHSFWPEGGRSTSKR
                     ISLLDVQLLKDMNMNAVRGHYPPDDHFLEVCDSLGLFVLNELAGWQNSYDTKTGTKLV
                     TETVVRDVNHPSVIIWDNGNEGGWNYDVDKVFDENDPQKRIVIHPWSDFNGWDTHHYP
                     TYLTGMHRFNSGENVFFPTEFMHGTYDNGMGAALEDFWTRYKKSPLFAGGFMWAMLDE
                     AVFRSDWKGEAKFDSKGSLAADGILGPHREKEGSYYTV"
BASE COUNT         2286 a         1497 c         1467 g         2438 t
ORIGIN      
        1 tccccgcaat tgcaactgct gaaatagtta catcctgttt ttcttcccat tttgttccca
       61 tatcacccca tagtcctgtt ggataaggaa ttcgcaaacg aattttcaag cgcccttgtt
      121 gtactaaatc ggattcgact tttactccaa tggcatcttt ttcttggtgc gaaagggttt
      181 ttacttttac ccaaacgcct tcaaccttga aagaactttc tatttcaccc gtccaaaggt
      241 tcaaggtttg atcaatagat tcgatgtctt ctgctttcgc caaagtcccg tcttttttag
      301 tgatttcaaa ccccaaatta cccaattgca gtaaatgtgc attttgtcgg aaccagttta
      361 cggcctcgtt ttttcgtggc gttgattttt cttgaacact ataactaatt tttctaccat
      421 attgatcgta gtctcttaag gtttccgaaa atttgtagtt attggtgttt ttataactat
      481 cccaacccca ttcggattgc gttcccaatg gtactccttt ttgatatttt tcaggaaaag
      541 tttgtaatcc agtggcgtca acagtaaagg caaaagctcc attcccgaca gacaaagaag
      601 ccaaagtatc gattgcagtg ttttttacat tatgccttgt gaccaatgct tttcggtcaa
      661 ttttttgctg tcctatcatt gcgatagaaa ataggaaagc ggtaagtagc aggttttttt
      721 tcatagttta atagtttttc tttgtaatta tttaccgcaa attcgcaaat tatttattga
      781 attgagatga atttaatttg cgaatttgcg gtttaagttt tgttttatcg aaccaaagtc
      841 gcactcatca atccaatcac cacgtcattg gcaatggatt tcagtgtgat gtttttcaat
      901 gttttatttt tgttcaaagg caaatccaaa atcgtcgccg caccgccatc aaccgcataa
      961 ttgctgaatc cttttatgct gctaaagtct ttaaaagttc tggaaatcgt tcccgatttc
     1021 agatacactc ttggtggttt tggtgcatcg gtggtaaaag ccaatccgtt tgtaaaataa
     1081 tcttgctcta tcggccacca gttttgagga tttttgagtt gcaaaacttc cgaacttccg
     1141 tcagtgtaat ttactgttat ttcgccgttt acaaatctac tttgcattgg attggtagtt
     1201 cctgccatca tcaaataaag gtgcgacgct tttcccgaaa ggggaacggc aatgctgtct
     1261 ggaaaaatat cccacatcga ggtaaaggca atgttttttg aattggcatc agcaggagtc
     1321 tggaacaaaa ttccttctgg actcgtgatt tgattgctgt tttttgcatt ttgacgtaag
     1381 cctgaatcat caatttttac atctaaatta ggataacacc aatttccgat gccgtgtttt
     1441 ggcaattgca aagtaggcga tgtcggtcta ggcgaaaggt attcctgctt gaaaatatcg
     1501 gtcactttcg aattgaattt atcagaaagt gaaattgttt ctaattttgt ttcttttggt
     1561 ataacaatat cccaattggt gaaagaaagt gaagttgttt tgccttcatt ggtttttaat
     1621 tctacagcat tcgtacctga aatcaaattt tcgaacggaa tggaaatagt ggtttcagaa
     1681 tctttattta aacttacttt ctgtgaaatt tcctttccaa aaggagtgaa aatcaaattc
     1741 ccgtcaatcg ctttttgact gttgtttttt atagcaaaaa gcaaattgtt gttttcaatt
     1801 cgagtcgatt ttacttcgat ttctggtttt acaatcaggt taatcggact ccaccaagtt
     1861 gtgtttcctt gttgtagttt tacaaaaaaa gtagcgtctt ctttggtttt taaattggct
     1921 tttagttcag aattggtttt cgaaatttcg cttaaagccg tttgtgggtc ataaatatcg
     1981 atgattttgg cttttttagt ttgcaattta atgctttcgc caatgcaata tactttatct
     2041 agttgcggtt cttctaactt ttcgccttcc caagtaatag atactttata actttcatta
     2101 tacggacttt cgacaatcaa ttgtggactt ccaaccgcat cagaaatggt tttccaagtt
     2161 gctttttcgc cattgatagt taccgatttt atgttttcat aaggaatttt tgccacgaat
     2221 ttcaaatcca tttttgttgt aaaatgagtt ttaatttcat aagaatccgt gtttttttct
     2281 ctagtaaaag caatcgaagc atcaggaatt tcaatacttg cagaattcca ttcgacggga
     2341 aatccgggtt gaatagttaa cctattttcc agcgcattgg gttgaattcc aaaaagtcct
     2401 tcgaccaagg ttcgagacgt cattccaatc gggtcggcaa aatcacggta caattcgcca
     2461 cgcatggcat catagaatga aagttgttcg aatccgcccg gcgaagcacc caaatacata
     2521 cttccgatca aggcactttc ccacattttg aaagcctttt ccgattgtcc gccttgccaa
     2581 aaagccaatg aagtgtgcaa ttgttccgcc aatgccacat tgttgagcga ccaatcatag
     2641 ggttgccaat tggtcgtgct gatggcgtat aaatcttttt tgtccaatcc ttcggcagtc
     2701 attggaatat gcggaatttt agcatccaaa taacgcaaca tctgataact ctgaaacgga
     2761 ttagataatt ccgaatcgat ggtgtgataa atactccaaa ctccaggcga atcgtgtact
     2821 aattgattgc ctaacgcatc tttgtattcg gcaaaaattc cttttttggg catccaaagt
     2881 tgggaattgg ttgcatcgaa aatctttttg gattcgttca aaaaaacgga attgtcttgt
     2941 tttaaatatt tggcgacagc cgctacttct ttgttcgaat aataattgta agccgacgaa
     3001 tgcgttaccg ctccgccgct gtattgcaag gcatcacttg cccaaatggc agcataagca
     3061 tcatacaagc catcgttatt ggtgtcgaaa tttctttttt cccaagccaa atgtctttga
     3121 atcacaggcc acatttcttt cataaaagta acatctcctg tccatttaaa atgacgcaac
     3181 atctggtcga aaaaaaccaa attcatatca taatgatgcg caatggtgtt gttgtttggt
     3241 cgcctgctga tgtaaccgct ggtaaacaat gtagttccca ttttttcggt ttctcgggct
     3301 aaatttcggc taacgtctgg tgtaacagga ccactcaaag gttcggtaac ttgcgatttg
     3361 ctgtaacttg taaaatgcga tttggctcgg tcgtgccagc ccaaaggatc agcagtataa
     3421 gcgcctcgcc aggcattgag gtacattctc caagcgatgg caccgtgcaa ataagcggga
     3481 ctttcccaaa ttccgtcagc agcaatcgcc aaggcagaac ccaaagtatt gatgtaagga
     3541 ttaggtgtga caactttgat acgattggcc aaaagttggg tttcttgaac agaattctca
     3601 aaaattgtgg ccaattcttt ttggtttttc aattgggttt tagcatcggt ttctatcatc
     3661 caaaacaaag ggtttttgga aatagttgct atttttccag tgataacagt caaggattct
     3721 ggtgcggaac caaacaattc caaaggcgat ttttgctgat tggcattcgc caaatgaatt
     3781 tcggaattag gataaaaacc aatcaaactt ttggcgtttc ccgttttttc tttattgact
     3841 tcagaaccat attctaaaac aaaagattct ttttgaactg tatatttatt gttggtacaa
     3901 taattaggca gcatataaaa aaccgattct ggatcggcac caatatcacc atcacgactg
     3961 aattttttgc cacttgctcc gccaaaagcc caaatcaaag aaaggtcatt tgatatatta
     4021 tttcctgtaa cttttagtcc aaaaccttcg gtgtttttta aggcgattac ttccaaaagt
     4081 agttctccgt tccctaaaat tgggtcttgg attttgtagt acatcattcc caaagtgtat
     4141 cgagcgtcga ttttcgtggc ttcggtaatc catttacttt ggtctccttt ttgaattccc
     4201 aatttaaaat ttccacccat tcccggcaaa tacaatgcaa attcgggcaa atcgccagtt
     4261 tctactcgaa aagcggtgtt ggttccgtaa agtgctctgt tgaattttcg actcccattt
     4321 ttcaagacaa aactatttcc ctctggagca taatgaattt tacgttccga tggcaagttt
     4381 ttttcttggc tcagagacgc taatggaaaa ctgagtaata aaacaaatat ccaaaaaaaa
     4441 gttagtttta gttttatttt aaacaattgc atgatttttg attgaaaaat aattaaatct
     4501 tagatagaaa tttagtaata attaagcaat ttcactatta ttattgttta atctatcctg
     4561 ttcttacctg atttttttta aataattgtc gatttcattt tttttggtag tataacctaa
     4621 tagtacagga cagtatatta atatggaaca tttatttttg aattattgtt gaatttaaat
     4681 ttgataaaat tgaataaaat ttacccgctt ttagcattaa ttgtttgctt ttttctgcca
     4741 ttcaaaagtt tggcttctga aacaaatagt agttcacaga tgatgaatgg ttcaaaatct
     4801 caaacgtttc attttgggac taaaaaatct tcttcaaaag taattggcat ttccaattca
     4861 ttggtttaca attcgaacac cggttatggc tttgattttg aaacggctgc gaatgtgaaa
     4921 atggactcta atactttttc aatagaaaaa ccaacctatt tttcggttaa acttcctgaa
     4981 gggaattata aagtggaagt aactatcggc agcaaagaaa aagcatccaa tgttaccatt
     5041 aaagcggaat ccagacgatt gatgctcaat caattttcag taaaaaaagg aagccaaatc
     5101 accaagactt tcaatgtaaa tttaagattt ccccaaattg acgctacttc caaaatcagc
     5161 ctaaaagaca gagatttaag cgttttcaat tgggacgaca aactcactct cgaatttttg
     5221 ggggttgcca acatccaaag tattaaaata acacctgtaa caactattcc cgtgatttat
     5281 ttggccgggg attctaccgt aaccgaccaa gatgtggaac cttgggcttc ttggggacaa
     5341 tttatcacca actattttga cgataaagtc gtggtggcca attatgccgt ttcaggttcg
     5401 gctttgagtt cttttaaggc cagcaaccgt ttgaaaaaaa taaattcact aatcaaaccg
     5461 ggcgattatt tgtttattga atttgcccat aacgacgaaa aactaaaagg ccctgaaaac
     5521 acggcttggg aatcctattc caaatattta gaagaatatg ttgaaatggc aagaagtaaa
     5581 ggagcatttc caattttagt aacaccaacc caacgcagag ctttcaaccc agacggaact
     5641 ttgaaaccaa cccacggcga ttttcccgat gcaatgcgag ccgttgccaa aaaaatgaat
     5701 gttccattga ttgacgttac tcaaatgacg acccaattgt atgaaaaatg gggagacgga
     5761 ccttccagaa aagcctttgt gcaatatcca gcaaatactt tcccgggaca gctaaaagca
     5821 ttggaagaca acacccattt caacagtttt ggagctaacg aaattgccct ttgcgtaatc
     5881 aagggcattc gagaattggg acttccctta aaaaataaca tcaccaagga aacacctgct
     5941 tacgacccga caaaacccaa ttctttttcg aattggactt tgccaatgag tgcccgtttc
     6001 gaaattgtta aacctgatgg aaattaaatc gctattgaaa aaaccaatta tgttacttaa
     6061 aaaaaacctt caattcattg ctattgctgc gctgacaact ttttctgcgc tacatagttt
     6121 cgcccaacaa accgacaaag tgttcctgtc cggaaaagat tttgaacatc ccgtgcaatg
     6181 ggatttttat tgcaccgatg gcaacaacag caaggcttgg tctaaaataa atgtgccttc
     6241 gcaatgggaa ttacaaggtt ttggaactta tacctacgga agatggtaca aagaattgaa
     6301 tgaaaaagaa ccgagcaaag aagaaggttt gtacaaatat gaattcgaag tgcctgccga
     6361 ttacaaagag aaagatattc taatcgcttt tggaggcgca atgaccgata ccgaagtgaa
     6421 agtcaacgga aaattggccg gcgaaattca tcaaggtggt ttttatgaat ttaaatacga
     6481 tattacgccg cttttgaact ttggaagcaa aaatattctg gaagtgcacg tttggaaaca
     6541 ttcggccaat ccatccgtaa atgctgccga acgcagagcc gattggtggt tgtttggcgg
     6601 aatttatcgc ccggtttggt tagaagtttc acctaaaacg caaatccaac atattgctgt
     6661 aaatccaaaa atggacggtt ccatcactgt cgatatgaac cttaaaaata ttgcaaaaaa
     6721 taccattgtc gaagcgactt tgaaaggctt ggacggcgaa aaattcgata ctttttcttt
     6781 tccaatcaaa gtcaaaagca ccaaagaaac catcgcagcc caatggaaaa atgtaaaacc
     6841 gtggaatacc gaaactccga atttatacaa cttggaattg gttttaaaac aaaacggaac
     6901 tctgattcat aaagtcgaaa aacgaattgg tttccgaact ttggaattca aaaagaaaga
     6961 cggaatctat gtgaacggca ccaagattgt aatgaaaggc atcaatcgcc actctttttg
     7021 gccagaaggt ggtcgcagta ccagcaagag aatcagtctt ttggacgtac aattattaaa
     7081 ggatatgaat atgaatgccg tgcgtggtca ttatccgccg gatgaccatt tcttggaagt
     7141 ttgcgattcc ttgggacttt ttgtgttgaa tgaattggcg ggttggcaaa attcatacga
     7201 taccaaaaca ggtaccaaat tggtaaccga aaccgttgtt cgcgatgtca atcatccttc
     7261 ggtgattatt tgggacaatg gaaacgaagg cggttggaac tacgatgtgg acaaagtctt
     7321 tgatgaaaat gatccccaaa aacgaatcgt gattcaccct tggtcggatt tcaatggttg
     7381 ggacacgcac cactacccta cttatttaac gggaatgcac cgttttaata gtggcgaaaa
     7441 tgtgtttttc ccaactgaat ttatgcacgg aacctatgac aacggaatgg gagccgcttt
     7501 ggaagatttc tggacgcgct acaaaaaaag ccctttgttt gccggcggat ttatgtgggc
     7561 aatgctcgac gaagctgtct tccgttcgga ttggaaagga gaagccaaat ttgattccaa
     7621 aggttctttg gcggccgacg gaattttggg accacaccgc gaaaaagaag gcagttatta
     7681 taccgtaa
//
LOCUS       DAOSNN010000116         7675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9531_length_7675_cov_4.473360, whole genome shotgun sequence.
ACCESSION   DAOSNN010000116 DAOSNN010000000
VERSION     DAOSNN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9531_length_7675_cov_4.473360"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1091)
                     /gene="fbp"
                     /locus_tag="PLQ70_07065"
     CDS             complement(72..1091)
                     /gene="fbp"
                     /locus_tag="PLQ70_07065"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQE34415.1"
                     /translation="MKENNLTLGEFIIENQKEFQYSSGELSRIFNSIKLAAKVVSYKV
                     NKAGLVDIIGGVGEKNVQGEDQQKLDVYANEVFIKTLINREIVCGIVSEENDDYITVK
                     GNDNSNNNKYVILMDPLDGSSNIDVNASVGTIFSVYRRISEVGTPVTLEDFLQPGTAQ
                     VAAGYVLYGTSTVLVYTSGHGVNGFTLNPAIGTFYLSHPKMTFPKDGTIYSVNEGNYI
                     HFPQGVKNYIKYCQTEEGDRPYTSRYIGGLAPDIHRNLIKGGIYLYPTSTKSPQGKLR
                     LLYECNPIAFIAEQAGGKASDGFSRIMEIVPTALHQRCPFFCGSANMVDKVEEFMLDA
                     KMSKY"
     gene            complement(1124..1858)
                     /locus_tag="PLQ70_07070"
     CDS             complement(1124..1858)
                     /locus_tag="PLQ70_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HQE34416.1"
                     /translation="MKFITLFLLLISLNCFAQKPTIYGIGDSTMANKTKPEENPERGW
                     GQMLPLFFNDNITIDNRAVNGRSTRSFINEKRWDDILKTLKKGDYVFIQFGHNDQKEK
                     DSTRYTNPHTAYRHNLIRFVEETRAKGATPIIFSSIVRRNFNEKGVLISTHGEYTMEA
                     RLVAQEYNVPFIDMEYLTELLEQSYGPEKSKQLHLHYKAGEIPYYKEDKADDTHLCVK
                     GATEVAKIAVEELKKTKLDIIKYIKP"
     gene            complement(1886..3235)
                     /locus_tag="PLQ70_07075"
     CDS             complement(1886..3235)
                     /locus_tag="PLQ70_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HQE34417.1"
                     /translation="MKYKFIVLLLISFHCFAQNNSFPTDKVNEIVQRIQLPTIPSYQI
                     VVTKLGAKGDSISNAKPAFDKAMALCKKNNGGTIIVPKGVYTLNGPIHFVSNVNLHLE
                     DGAKIRFGSNAKDYPLVLTSWEGTILYNYSPLIYAYNVENIAITGNGIIDGEAKNTWI
                     KWKPLEKNDQQLSREMNHKNTPVKERIFGQGHYLRPQLIQFFNSKNILVENVKIEDSP
                     FWCIHLLKSKSITIRGVKYNAQNNNNDGVDPEYSSDILIENVLFDNADDNVAIKAGRD
                     DEGRSNSNTPSENIVIRNCEFKGLHAIVIGSEMSAGVRNVYVENSKYRGYLKRGIYIK
                     TNSDRGAYVKDLYFNNLAFGKVEDCLYITANYHGEGSGLFPTKISDVSFSNISCLEAT
                     NTGIVIEGYPDKKVANIKLDNINIQSAKNGMTITNTENVTTNEVVIGTKATIPTAIK"
     gene            complement(3241..4092)
                     /locus_tag="PLQ70_07080"
     CDS             complement(3241..4092)
                     /locus_tag="PLQ70_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HQE34418.1"
                     /translation="MNAVKGLPSERVTLFIKNGVYYEKLIIPALFPKISLIGESREKT
                     IITYDDYSGKPSNIRIGTSNSYTVLIEGNDFYAENLTIQNTAGTVGQAVALNINANRA
                     IINNCSLLGNQDTLFTSAGRAVNYFKDCYIEGTTDFIFGDATTLFENCTIHSKKNSYI
                     TAASTPQESTFGYVFKNCKLTADENTTQVYLGRPWKNFAKTVFMNCEMGKHVRPEGWH
                     NWSKPDAEKTTFYGEYKCTGAGFQPKKRVAWSHQLKRAEAQKYTMENILGLDFSKTIK
                     EMYSIKK"
     gene            complement(4194..5279)
                     /gene="pelA"
                     /locus_tag="PLQ70_07085"
     CDS             complement(4194..5279)
                     /gene="pelA"
                     /locus_tag="PLQ70_07085"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HQE34419.1"
                     /translation="MKKLKIALVFALILVLNTTIYAQVSNKKWPDIINKSEPAWFATA
                     EAKAVAENVLLYQRNIGGWPKNIQMQNPLSEVEKQKLIALKPDTKEITTDNRATTQEM
                     LFLSKMYAQTPDERYKAAFLAGFDYLLKAQYANGGWPQFYPLKKGYYTHITYNDDSMA
                     NILMVLKECIDKTDYFSIKPTESTLEKAKIAFNKGIDCIIKSQYKQNGVLTAWCAQHD
                     EVTLLPANARAYELASLSGQESVKIVQLLMSIENPSPEVINAVNSAVAWFEKTKITGI
                     REDRVPVPNSKIMEKVLVKDPNAEPLWARFMELTDNTPFFCDRDGIKKASYFEIGQER
                     RTGYNWYTSKPNDVLKKYPAWKKKLKL"
     gene            complement(5289..6920)
                     /locus_tag="PLQ70_07090"
     CDS             complement(5289..6920)
                     /locus_tag="PLQ70_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HQE34420.1"
                     /translation="MKFIKITSLFLCSLALQNSIAQEKSETNRSQVWVADNGDGTYKN
                     PIINADYSDPDAIRVGDDYYLTSSSFNCIPGLPILHSKDMVNWELVNYALKKQPPFDV
                     YDKPGHGNGVWAPSIRFHNEEFYIYYPDPDYGIYMIKTKDPKGDWSEPVMVKEGKGLI
                     DPCPLWDEDGKVYLAYAYAGSRAGFKSLLAICSLNPEGTSANYNDDVIIIDGHDGEAT
                     IEGPKLYKRNGYYYVFAPAGGVPTGWQTVLRSKNIWGPYEKRKVLDQGKTKVNGPHQG
                     AWVQTQTGEDWFFHFQDKFAYGRVVHLQPMKWIKDWPVIGTDKDGDGTGEPVATYKKP
                     NVGKKYPISTPPESDEFNSPKLGLQWQWHANQQVYWGFPSTMGYYTMYCRPKPENYIN
                     LFDVGNLLLQKFPADEFTATTKMTFNARFDDEQTGLVIMGLDYSYLKVKQTAGQLSIS
                     QVICKNADKKGAEKESESIKLKSNTFYLQVKVKAGGICNFFYSEDGKKFTAVGTDFTS
                     REGKWIGAKLGFLALRNGNINDAGSVRIDWFRITK"
     gene            complement(6934..>7675)
                     /locus_tag="PLQ70_07095"
     CDS             complement(6934..>7675)
                     /locus_tag="PLQ70_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HQE34421.1"
                     /translation="GGFWHKQIYTNQMWLDGLYMGEPFYAQYTATFENGKNLDDVAKQ
                     FEEIQKHATDPKTGLLYHGWDSEKLMPWANKETGNSPNFWSRALGWYAMALVDALDYF
                     PKDHPKQKELVGYLNNVSASLAKFQDKKTGLWYQVTDKGGKEGNYLEGSGSAMFAYAF
                     AKGANKGYLPSKYKKLANKAFDGLTTKLMKIDADGTITITDVCAVAGLGGTPYRDGSY
                     EYYVNEKKKDNDPKATGPFILAALELNR"
BASE COUNT         2043 a         1436 c         1458 g         2738 t
ORIGIN      
        1 aataaaaaaa gctccgaaag gagcttttta gtttgttgtc aatctgattt ttacaaatct
       61 gcaatctgtt tttagtattt actcattttt gcatcgagca taaattcttc cactttatct
      121 accatattgg cgcttccaca aaagaatgga catctttggt gcaatgcggt tggaacaatt
      181 tccatgattc tagaaaaccc atctgaggct tttccaccgg cttgttctgc tatgaaagca
      241 atagggttgc attcgtataa aagacgtaat ttcccttgag gcgatttggt actcgttgga
      301 tacaaataaa tacctccttt tattaaattt cggtgaatat ctggagctaa tcctccaata
      361 tatctagaag tataaggtct atcgccttcc tcggtttggc aatatttgat gtagtttttg
      421 actccttgtg gaaaatgtat atagttccct tcatttacag aataaatggt tccatccttt
      481 gggaatgtca ttttaggatg agacaagtag aaagttccta tggcgggatt taaggtaaaa
      541 ccatttacgc cgtgtccaga ggtgtaaacg agaacagttg aggttccata aagtacatat
      601 cctgcagcaa cttgtgcagt tcctggttgt aaaaaatctt ctagtgttac tggagttcct
      661 acttctgaaa ttcttctata aacggagaaa atggttccta cagaagcatt cacgtcaata
      721 tttgaagaac cgtccaatgg atccatcaga atgacatact tattgttatt gctgttgtca
      781 tttcctttaa cggttatgta atcgtcgttt tcttcagaaa caataccaca tacaatttct
      841 ctattgatta gtgttttaat aaaaacctca ttggcataga catctagttt ttgctgatct
      901 tcgccttgta catttttctc tccgacacct ccaataatat ccaccaatcc agctttgttc
      961 actttgtagc tgactacttt tgcggccaat ttgatggaat tgaaaatgcg cgacaattcg
     1021 ccggatgaat attgaaattc cttttgattt tcaataataa attctcctaa agttagattg
     1081 ttttctttca tttgagtttg gtttagtagt agttgttttg attttatggt ttgatatatt
     1141 ttatgatatc gagtttggtt tttttcaatt cttctacagc gatttttgcc acttcggtag
     1201 ctccttttac gcaaagatgg gtgtcatcgg ctttatcttc tttgtaataa ggtatttcac
     1261 cagctttata atgcaaatgt aattgttttg atttctcagg accatacgat tgctctaata
     1321 attcagtcaa atattccata tcgatgaagg gaacgttgta ttcttgagca accaagcggg
     1381 cttccattgt atattcgccg tgcgtagaaa tcaaaacgcc tttttcattg aaatttcgtc
     1441 gaacgattga agaaaagata attggtgttg ctcccttggc tctagtttct tccacaaatc
     1501 gaatcaaatt gtgtcggtaa gccgtgtgtg ggttggtgta acgggttgaa tctttttctt
     1561 tttggtcatt gtggccaaat tgaataaaaa cataatcgcc tttttttaaa gtttttagaa
     1621 tatcatccca acgtttttcg ttgataaaac ttcgggtgct tctaccattt acggctctat
     1681 tgtcgatagt gatgttgtca ttaaaaaaaa gcggcaacat ttgtccccaa ccgcgctctg
     1741 gattttcttc aggttttgtt ttattcgcca ttgtcgagtc gccaatgccg taaatagttg
     1801 gtttttgtgc aaaacagttt agtgaaatta acaataaaaa aagtgtaatg aatttcatgg
     1861 tatttagttt ttgagtttta attatttatt tgatagcggt tggaatagtg gcttttgttc
     1921 caataacgac ttcgtttgta gttacatttt cagtgttggt tattgtcatt ccgtttttgg
     1981 ccgattgaat gttgatgttg tccagtttga tgttggctac ttttttgtcg gggtatcctt
     2041 caatcacgat tccggtatta gtggcttcaa ggcaactgat attcgaaaaa gaaacatcgg
     2101 aaattttagt gggaaataat ccgctgcctt ctccgtgata attggcggta atgtaaagac
     2161 aatcttccac ttttccgaat gccaggttgt tgaaataaag gtcttttaca taagctccgc
     2221 gatctgaatt ggttttgatg taaatgcctc ttttcaaata accacgatat ttgctgtttt
     2281 cgacatatac atttcgaact ccggccgaca tttcgctgcc aatgacaatg gcgtgaagtc
     2341 ctttgaattc acaatttcgg ataacgatat tttcagatgg ggtgtttgaa ttgctacgac
     2401 cttcgtcatc tcttcctgct ttgatggcca cattatcatc ggcattgtca aaaagaacat
     2461 tctctattaa aatatcgctg gaatattcag ggtcaacgcc atcattattg ttgttttggg
     2521 cattgtattt cacgccacga atggtgatgc ttttgctttt tagcaaatga atgcaccaaa
     2581 aaggagaatc ttcaatcttg acattttcta ccagaatgtt tttcgaattg aaaaactgga
     2641 tcaattgcgg tcttaaataa tggccttgac caaaaatccg ctccttgaca ggcgtgtttt
     2701 tatggttcat ttctcggctc aattgttggt cattcttttc gagaggtttc cattttatcc
     2761 aagtgttttt ggcttctcca tcgataattc catttccggt aatggcgatg ttttcaacat
     2821 tgtaggcgta aatcagtgga ctgtaattat aaagtatggt gccttcccaa ctggtcaaaa
     2881 ccaagggata atcttttgca ttggaaccaa atctaatttt ggcaccgtct tccaaatgca
     2941 ggtttacatt acttacaaaa tgaatgggac cattcaaggt gtaaactcct ttcgggacga
     3001 tgatggtgcc tccgttgttt tttttgcaaa gtgccattgc cttatcaaac gcgggtttgg
     3061 cattgctgat agaatcgcct ttcgcaccta atttggtgac cacaatttga taagaaggaa
     3121 ttgtgggtaa ttgaatacgt tggacaattt cattgacttt gtcggtcgga aagctattgt
     3181 tttgggcaaa acaatggaat gaaatcagca atagaacaat aaatttatat ttcataattt
     3241 tcatttttta attgaataca tttcttttat ggttttcgaa aaatccaagc ccaggatatt
     3301 ttccatcgtg tatttttggg cttcggctct ttttaattga tgcgaccaag ccactctttt
     3361 tttgggttga aaacctgcgc cagtacattt gtattcgcca taaaaagttg ttttttcggc
     3421 atcgggttta gaccaattgt gccaaccttc gggtctgacg tgtttgccca tttcgcagtt
     3481 cataaagact gttttggcaa aatttttcca aggtcttcca agatatactt gtgttgtgtt
     3541 ttcatcggct gtgagtttgc aatttttgaa aacataacca aatgtagatt cttgtggagt
     3601 ggatgcggct gtaatataag aatttttttt gctgtgaatc gtgcaatttt cgaatagagt
     3661 agtggcatct ccaaaaataa aatcggtggt accttcgata taacaatctt taaaataatt
     3721 cactgctctt cctgccgaag taaaaagtgt gtcttgattg cccaacaaag agcagttgtt
     3781 gataatcgcc ctgtttgcat ttatattcaa ggcaaccgct tgaccaactg ttccagccgt
     3841 gttttggatg gtcaagtttt cggcataaaa atcattgcct tctatcaaaa cggtatagga
     3901 attagatgtg ccaattctga tgttacttgg ttttcctgaa taatcatcat aagttatgat
     3961 tgttttttct cggctttcgc caatcaagga aatcttcgga aacaaggcag gaatgattag
     4021 tttttcgtag taaacgccat tttttatgaa gagggtaact ctttcggaag gaagtccttt
     4081 tacggcgttt atggcttctt gaatggtagt aaaatcgccg tttccgtttt tgtcaacgac
     4141 gattttatat ttgtcttttg gaacaattgt agtagtagaa actggtattt cagttacaat
     4201 ttcagttttt ttttccaggc tggatatttt ttcaaaacat catttggttt gctggtgtac
     4261 caattgtagc ccgttctgcg ctcttgtcct atttcaaaat aagatgcttt tttgattccg
     4321 tctctgtcac agaaaaaagg agtattgtca gtaagttcca taaaacgtgc ccacaacggt
     4381 tcagcattcg ggtctttgac taatactttt tccataattt tactgttagg aacaggaaca
     4441 cgatcttctc taatacctgt gatttttgtt ttttcgaacc aagccaccgc actgtttacc
     4501 gcatttataa cttctggaga tggattttcg atagacatca atagctgcac aattttaacc
     4561 gattcttgac cactcaagga agccagttca taagctctgg cattggctgg aagcaaagtc
     4621 acttcgtcgt gttgcgcaca ccaagcggtc aaaacaccat tttgtttgta ttgtgatttt
     4681 atgatgcaat caatgccttt attgaatgct atttttgctt tttcgagagt agattcagtc
     4741 ggttttatgg agaaataatc ggttttgtcg atgcactctt ttaataccat taatatgttt
     4801 gccattgaat catcattgta ggtgatgtgg gtgtaatatc cttttttcaa cggataaaat
     4861 tgtggccaac caccgtttgc atattgcgct ttcagtaaat aatcaaatcc ggctagaaag
     4921 gcagctttgt atctttcgtc tggagtttga gcgtacattt ttgacaagaa caacatttct
     4981 tgcgtggtgg ccctgttgtc ggttgtaatt tcctttgtgt cgggttttag ggcaattagt
     5041 ttttgttttt caacctcgct caatggattt tgcatttgga tatttttggg ccaaccacca
     5101 atattgcgtt ggtacaacaa tacattttcg gcaacggctt ttgcttcggc tgttgcaaac
     5161 caagcgggtt ctgatttatt gatgatatca ggccattttt tgttgctgac ttgggcataa
     5221 atagttgtat ttaataccaa gattaaggca aagacaagcg ctatttttaa ttttttcatt
     5281 gtagaaattt attttgttat tctaaaccag tcaattcgaa cacttcctgc atcgtttatg
     5341 tttccatttc gaagtgccaa aaagcctaat tttgctccaa tccatttacc ttctcttgaa
     5401 gtgaaatcag ttccaactgc agtgaatttt ttgccgtctt cactgtagaa gaaattgcaa
     5461 attccgcctg cttttacttt tacttgaagg taaaaagtat tgcttttcaa tttaatggat
     5521 tcactttctt tttctgcacc ttttttgtcg gcatttttgc agattacttg ggatatggat
     5581 aattgtcctg ctgtttgttt tactttcaaa tagctgtaat ccaatcccat tattaccaaa
     5641 ccggtttgtt catcgtcgaa acgggcatta aaagtcattt tggtagttgc agtaaattca
     5701 tcggcaggaa atttttgtaa taataaattc cccacatcga ataaattgat gtaattttca
     5761 ggttttggac gacaatacat cgtgtaatac cccatcgtgg aaggaaatcc ccaatacact
     5821 tgttgattgg cgtgccattg ccattgcaat cctaattttg ggctattgaa ttcatctgat
     5881 tctggtggag tggaaatagg atattttttt ccaacatttg gctttttgta agttgctact
     5941 ggttcgccag ttccatcacc atctttgtcg gttccgatta ctggccaatc tttaatccat
     6001 ttcataggtt gcaaatgaac gactcttcca taagcaaatt tgtcttgaaa gtggaaaaac
     6061 caatcttctc cagtttgtgt ttgcacccaa gcaccttggt gtggaccatt cacttttgtt
     6121 tttccttgat ctaaaacttt tcttttttcg taaggacccc aaatattttt tgatcttaag
     6181 acggtttgcc atcctgtagg aacaccacct gcaggtgcaa aaacataata gtagccattg
     6241 cgtttgtata attttggacc ttcgatggtg gcttcgccat cgtgaccgtc aataatgata
     6301 acatcgtcat tgtaattggc agaagttcct tcaggattta gactacaaat ggccaaaagg
     6361 cttttgaaac ccgctcggct tccggcataa gcataagcaa gatagacttt gccatcttca
     6421 tcccataacg ggcaaggatc aatcagacct tttccttctt tgaccataac gggttcagac
     6481 caatcacctt ttgggtcttt ggttttaatc atataaatac cgtaatcagg gtcgggataa
     6541 taaatataaa attcctcatt atgaaatcta atggacggag cccaaacacc atttccgtgt
     6601 cctggtttgt cataaacgtc gaaaggaggt tgctttttaa gcgcataatt aaccaattcc
     6661 caattgacca tatccttgga atgcaaaatg ggcaaacccg ggatacaatt gaaagaggag
     6721 gatgtcaaat aataatcatc gccaacacga atggcatctg ggtcagaata atcggcattg
     6781 atgatggggt ttttataggt tccgtctcca ttgtcggcca cccaaacttg ggaacgattg
     6841 gtttcgcttt tttcttgtgc tattgagttt tgaagtgcca aactgcacaa aaaaagcgaa
     6901 gttattttta tgaatttcat ctagttaagc ggattatcta tttaattcta aagctgccaa
     6961 aataaacggt ccagttgctt ttggatcatt gtcttttttc ttttcattta cataatactc
     7021 ataagaaccg tctctgtaag gtgtacctcc aagtcctgca acggcgcata catcagtaat
     7081 ggtaattgtc ccgtcggcgt ctattttcat caatttggtt gtcaaaccat caaaggcttt
     7141 gttggccaat tttttgtatt ttgagggtaa atatcctttg ttagctcctt ttgcaaaagc
     7201 ataagcaaac attgcagaac cagaaccctc taaataattt ccttctttac ctcctttatc
     7261 ggttacttga taccataagc cagttttttt gtcttgaaat ttagccaaac tagccgaaac
     7321 attgtttaaa tagccaacca attctttttg ttttgggtgg tctttaggga aataatctaa
     7381 agcatctaca agtgccattg cataccaacc caatgctctt gaccagaaat tgggtgagtt
     7441 tccagtttct ttgtttgccc aaggcattaa tttttcagaa tcccaaccgt ggtataataa
     7501 tcctgttttt ggatctgttg cgtgtttttg aatttcttcg aattgtttgg caacgtcatc
     7561 taagtttttt ccgttttcga aagtagcagt gtattgagca tagaatggtt ctcccatata
     7621 taaaccatca agccacattt ggttggtgta aatttgtttg tgccaaaacc cacca
//
LOCUS       DAOSNN010000117         7645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9583_length_7645_cov_4.911199, whole genome shotgun sequence.
ACCESSION   DAOSNN010000117 DAOSNN010000000
VERSION     DAOSNN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9583_length_7645_cov_4.911199"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLQ70_07100"
     CDS             <1..466
                     /locus_tag="PLQ70_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LETM1 domain-containing protein"
                     /protein_id="HQE34422.1"
                     /translation="DGIVEKNEAYFLYKLAELMEVPDDFVSESIENTNKFITKFKKEI
                     PYFNYSNPVKHFYDQTTQTVVKLITRNKNRLVKEILESKELMVLLAYSTRRDLNEKEK
                     KKVRKQLLDICKTIPSLAIFLLPGGSLLLPIFIKFIPTLLPSSFNENLENED"
     gene            complement(508..2514)
                     /locus_tag="PLQ70_07105"
     CDS             complement(508..2514)
                     /locus_tag="PLQ70_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HQE34423.1"
                     /translation="MRKIIFVLLVFLAFAKGNSQILDPVKWTTKIEKKSETSFLLSFN
                     GVIEKDWHMYSQFTPEGGPLPMEVIFKDQKGNYKLIGKAKEGKTTTAYNDVFEVNETF
                     FVKNASIQQEISLINPKIKTIEVALNYQVCNQSCINLEKKFSFVIPTTSLTEIVPTQI
                     DTVKNEVIDSKSVEKIKPEVAKSNEIIATSEDAPQKGLWSIFLAALLAGFTALLTPCV
                     YPMIPMTVSFFTKQSKTKSQGKRNSIIFGMAIILIYIILGSVITALFGASALNEFSTG
                     VTFNLIFFLLLVIFAISFLGAFEIMLPNSWANKVDKQADKGGIIGILFMALALAIVSF
                     SCTGPFVGNILFASASVGGIGPIIGMLGFSSAFALPFVLFAFFPAWLQSLPQSGGWMN
                     TVKVFLGFLELALAFKFLSNADLVLQMHLLEREVFLAIWIAIFGALAFYLFGKITLPH
                     DSPIAHISVGRLLLGLVVLSFTIYMIPGLWGAPLKLINAFPPPMEYSESPLGFGNSGN
                     NSSTAVLPDGAKLGPHQIVVFNDYEKGLAYAKTVNKPIMLDFTGFACVNCRKMENNVW
                     SDQRILPILKKDIVLISLYVDDKRELPTTEQFISKTTNEKIETIGDKWTDFMITKYKT
                     NTQPLYVLTDLDGNSLNTITPTISYTSVEDYENWLKTGISKFKK"
     gene            complement(2514..3824)
                     /gene="tilS"
                     /locus_tag="PLQ70_07110"
     CDS             complement(2514..3824)
                     /gene="tilS"
                     /locus_tag="PLQ70_07110"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQE34424.1"
                     /translation="MQETFENHIAQNFPFLIGKNLLLATSGGLDSMVMVDLFHKLPFE
                     IAIAHCNFQLRGIESYGDQNFIQNYAETNEIKLFLTQFDTEAFANDYKLSTQVAAREL
                     RYSWFYELLETENYDYILTAHHADDNLETFLIHLVRGTGIDGLTGIPSKNGQVIRPLL
                     IFSRSEIAQYAEENAIQWREDSSNASDKYLRNKIRHNLVPILKELNSDFLTTFQKTQT
                     YLQESKAMIADASFLIYQQVAKEKGTEIHFDLIQLKKLPNYKSYLYHWFNEFGFSAWE
                     DIYDLIDGQSGKQVFSSEFRLLKNRDFLILSPINLEDEYEEFFINKNQKEVNVPLNLT
                     FCKVDNISMASNTTIFVDEDKIWYPIVLRRWRDGDTFQPFGMDGKSKKVSKFFKDEKL
                     SLIEKEKAWLLCSDNEIVWIVGIRQDERFRVKSTTKNILKIHLK"
     gene            complement(3897..5183)
                     /locus_tag="PLQ70_07115"
     CDS             complement(3897..5183)
                     /locus_tag="PLQ70_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HQE34425.1"
                     /translation="MRTSIIKHIENPALFKQHLLTWSQQFREVVFLDSNDYPQNYSSY
                     DCLLAVDAFTSIKTDYHSAFEDLKQYQQATKDWLFGYLSYDLKNNVEVLDSNNFDGLN
                     FPDLFFFQPKKLFLLKGNQLEIQYLNLCDDEVSGDIEEISIQCSVFSVQSQEIKIQQR
                     ISKESYLDKAAKFLEHIHRGDIYEANFCMEFFAENAIITPIDIFLKLNEISKSPQAVF
                     FKNKECFLLSASPERYLKKEGNLLISQPIKGTAKRFTDTIEDEKSKNNLALDPKERSE
                     NIMVSDLVRNDLSRTAEKGSVKVEELCGLYSFKQVHQMISTITSRLDSQYSAIDAIRT
                     TFPMGSMTGTPKVSVLKIIEELEETKRGLYSGAVGYFAPNGDFDFNVVIRSILYNQEN
                     KYASFSVGSAITAQSIPEKEYEECLLKAKAMREVLS"
     gene            5278..5655
                     /locus_tag="PLQ70_07120"
     CDS             5278..5655
                     /locus_tag="PLQ70_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQE34426.1"
                     /translation="MKILTIIIRTAIGLLLFSVAIGYFLKLTPEPVTNGNFKAFEIGF
                     VSSEYLIPIVKFVTLLCGLAFVFDRYITIASLLILPFTINALFLDYYLTPQALPVSIF
                     VFLGNFFIIYLHRKNYKKLFKVK"
     gene            5821..6714
                     /locus_tag="PLQ70_07125"
     CDS             5821..6714
                     /locus_tag="PLQ70_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HQE34427.1"
                     /translation="MIKTSKTILALLIVIFAISCKKAEEASESAYESNTIAVDTTNTI
                     SSSAAVEPKNSDRKFVRTADIKFKVKNVANSTYAIENTANKFGGFVTYTNLQSTVSDK
                     QETKISQDSTLEITKYTVENNITIRVPNIRLDTVIKTIAKQIDFLEYRIIKADDVSLQ
                     MLSNQMQQNRSSNQEKRLEKAIDTKGKKLNEVIAAEDNLNTKNEQNDNAKLENLSLKD
                     QVNFSTLTLQIYQRETIKEEMVANPKTYRQGFGSKILDGIISGWYVIEGIIAVIAQLW
                     SVILIGIGGYIIFKKYFHKGN"
     gene            complement(6777..>7645)
                     /locus_tag="PLQ70_07130"
     CDS             complement(6777..>7645)
                     /locus_tag="PLQ70_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQE34428.1"
                     /translation="IGGGVIGIELGQVYLRLGAQVSVVEFMDKIIPGMDGALSKELTK
                     VLKKQGMKFYVSHKVKSVERNGDNVTVQAENAKGETITLEGDYSLVSVGRRPYTDGLN
                     ADKAGVKITERGMVEVNDHLQTNVPNIYAIGDVVRGAMLAHKAEEEGTMVAEILAGQK
                     PHIDYNLIPGVVYTWPEVAAVGQTEEQLKASGVEFKSGSFPFKALGRARASGDLDGFV
                     KILADKKTDEVLGIHMIGARCADLIAEAVTAMEFKASAEDISRMSHAHPTFSEAIKEA
                     ALAATENRALHV"
BASE COUNT         2615 a         1379 c         1106 g         2545 t
ORIGIN      
        1 tgatggaatt gtcgaaaaaa acgaagctta ttttctttac aaattagccg aattaatgga
       61 agttccagat gattttgtta gcgaaagtat agagaataca aacaaattta ttacgaaatt
      121 caaaaaagaa attccctatt ttaattactc caatccggtc aaacattttt atgaccagac
      181 cactcaaacc gttgtaaaac taattacccg taacaaaaat agattagtta aggagattct
      241 tgaaagcaag gaattgatgg tgctacttgc ctattctacc cgaagagatt tgaatgaaaa
      301 agaaaagaaa aaagtaagaa aacagttgtt ggatatttgt aaaacgatac catcattagc
      361 aatattctta ttgccaggcg gcagtttgtt actacctatt tttatcaaat ttattccaac
      421 gttacttcct tcttcattta atgaaaacct tgaaaacgag gattaaaaaa ttcaaattac
      481 accttttatt agagattgaa ttttagatta tttcttgaac tttgaaattc cagtttttaa
      541 ccaattttca taatcttcaa cacttgtata gcttattgtt ggagtaattg tattcaaact
      601 atttccatct aaatcggtca aaacatacaa aggttgtgta ttggttttgt attttgtaat
      661 cataaaatct gtccacttat caccaatggt ttcaattttt tcatttgtcg tttttgaaat
      721 aaattgttca gttgttggca attctcgctt atcatctaca tataaagaaa tcaaaacaat
      781 atcctttttt agaataggta aaatacgttg gtctgaccaa acattatttt ccatttttct
      841 acaattaaca caagcaaacc cagtaaaatc tagcattata ggcttattaa ctgttttagc
      901 ataagccaac cctttttcgt aatcattaaa aactacaatt tgatgtggcc ccaatttagc
      961 cccgtctggc aataccgcag tagaagaatt attacccgaa tttccgaaac caagagggct
     1021 ttcgctatat tccattggtg gtggaaatgc atttatcaat tttaatggcg ctccccaaag
     1081 cccaggaatc atataaatag tgaacgataa aacaaccaat ccaagcaata gccttcctac
     1141 tgaaatatgt gctattggac tgtcgtgtgg caaagtaatt ttcccaaaca aataaaaagc
     1201 caatgctcca aaaattgcaa tccaaatagc caagaacact tctctttcta acaaatgcat
     1261 ttgaagaacc aaatcagcat ttgataagaa tttgaatgcc aaagccaatt ctaaaaaacc
     1321 taaaaacact ttcacagtat tcatccagcc tcctgattgc ggcaaggatt gcaaccaagc
     1381 tggaaaaaat gcaaataata caaatggcaa agcaaaggct gaagaaaatc ctaacatacc
     1441 aataattggc ccaattcctc ctactgaagc tgaagcaaat aatatatttc caacaaatgg
     1501 cccagtgcaa gaaaatgaaa ctattgctaa tgccaaagcc ataaacaaaa taccaataat
     1561 tccaccttta tcagcttgct tgtccacttt atttgcccaa gaattaggca acattatttc
     1621 aaaggcaccc aaaaatgaaa tagcaaaaat cactaacaaa aggaaaaaaa tcaagttaaa
     1681 agttactcct gtagaaaact cattaagggc agatgcacca aatagagcag taattacaga
     1741 gcctagaatg atataaatta aaataatagc cattccaaaa ataattgaat tgcgtttgcc
     1801 ttgacttttg gttttgctct gtttggtaaa aaaactaacg gtcattggaa tcataggata
     1861 tacacaaggc gtcaataatg cggtaaatcc tgccaataac gctgcaagaa aaatactcca
     1921 caaccctttt tgtggtgcat cttcagaagt agcaattatt tcatttgatt ttgcaacctc
     1981 aggttttatt ttttctacag attttgaatc gattacttca tttttaacag tatcaatttg
     2041 tgttggtact atctcagtta gagaagttgt tggaattaca aaactaaatt ttttctccaa
     2101 attaatacaa gattgattgc aaacttgata atttaaagcc acttcaatag tcttgatttt
     2161 tggatttatc aacgaaatct cctgctgaat tgaagcgttt ttaacaaaaa aagtttcatt
     2221 tacttcaaaa acatcgttgt atgctgtggt cgtttttcct tctttggctt tgccaatcaa
     2281 tttataattc cctttttgat ctttaaaaat cacttccata ggcaatgggc caccctccgg
     2341 agtaaactgg gaatacatat gccaatcctt ttctataaca ccgttaaagc tcagtaaaaa
     2401 actagtttca gatttttttt cgattttagt agtccatttt acagggtcaa gtatttgaga
     2461 atttcctttg gcgaaagcca aaaaaacaag aagtacaaaa atgatttttc gcattatttt
     2521 aaatgtattt taagtatatt ttttgttgtg cttttaactc taaaacgttc gtcttgacga
     2581 atacctacaa tccaaactat ttcattatca gaacacaaca accaagcttt ttctttttcg
     2641 attaatgata atttttcgtc tttaaaaaat ttgctaacct tttttgattt tccatccatt
     2701 ccaaaaggtt gaaaagtgtc gccatctctc caacgacgca aaacaattgg ataccaaatt
     2761 ttatcttcat cgacaaagat agttgtattt gaagccatac taatgttatc tactttacaa
     2821 aaagtgagat ttaagggaac attaacttct ttttgatttt tatttataaa aaactcctca
     2881 tattcatcct ctaaattaat tggacttagt atcaaaaagt ccctgttttt cagcaatcta
     2941 aattcacttg aaaatacttg tttaccagat tgaccatcaa ttaaatcgta aatatcctcc
     3001 caagccgaaa aaccaaattc gttaaaccaa tgatacaaat aggatttata attaggcagt
     3061 ttttttagct gaatcaaatc aaaatgtatt tcggttcctt tttcttttgc gacttgctga
     3121 tagatcaaaa atgaagcatc tgcaatcata gctttagatt cttgcaaata agtttgagtt
     3181 ttttgaaaag tggttaaaaa atcggagttt aactctttca atataggaac taaattatgc
     3241 ctgattttat ttcgcaaata tttatctgat gcattactgc tgtcttctct ccattgaatg
     3301 gcattttctt cggcatattg agcaatttct gacctagaaa aaattaaaag tggtcgaatt
     3361 acttgcccat tttttgacgg aattccagtc aaaccatcaa ttccagtacc tcgaacaagg
     3421 tgaattaaaa aagtttctaa attatcatcc gcgtgatgag ctgtcaggat ataatcgtag
     3481 ttttcggttt ctaaaagttc gtaaaaccag ctgtatcgca attcacgagc agcgacctga
     3541 gtagaaagtt tgtaatcatt ggcgaaagct tcggtgtcaa actgagttaa aaacaatttg
     3601 atttcgtttg tttcggcata attttgaata aaattttggt ctccataact ttctatacca
     3661 cgaagctgaa aattgcaatg tgctatagca atttcgaaag gtaatttatg aaataaatcc
     3721 accattacca tactatccaa accaccacta gtagccaaaa gaagattttt tccaattaaa
     3781 aaaggaaaat tttgagcgat atgattttcg aatgtttctt gcatattcaa atttagttat
     3841 tttaaataat attttgaatt aactaagtga atattttgaa tcaacagaga attaattcaa
     3901 ctcaaaacct ctcgcattgc tttggctttg agcaaacatt cttcatattc tttttcagga
     3961 atagattgag cagtgatggc acttcctaca gaaaatgaag cgtatttatt ttcttgatta
     4021 tacaaaatac ttcgtataac tacattgaaa tcgaaatcac catttggagc aaaataacca
     4081 acagcgccac tatacaaacc tcttttggtt tcttccaatt cttcgatgat tttcagtact
     4141 gaaaccttgg gagttcccgt catgcttccc atcggaaaag tagttctaat agcgtcaata
     4201 gcagaatatt gactgtctaa tctggaagta atcgtcgaaa tcatttgatg tacttgtttg
     4261 aaggaatata aaccacataa ttcttccact tttaccgaac ctttttcagc ggttcttgat
     4321 aaatcatttc gaactaagtc ggaaaccatt atgttttcgg aacgctcttt tgggtctaat
     4381 gccaaattat tttttgattt ttcatcttcg attgtgtcag taaatctttt ggcagtgcct
     4441 ttgatgggtt gagaaataag caaattacct tcttttttta aatatcgttc tggtgatgca
     4501 gaaagtaaaa aacactcttt attcttaaaa aaaacggcct gaggagattt tgaaatttcg
     4561 ttgagtttta aaaatatgtc tattggggtg attatagcat tttcagcaaa aaattccata
     4621 caaaagttag cctcataaat atcgcctcga tgaatatgct ccagaaattt agctgcttta
     4681 tcaagataac tttctttaga aattcgttgt tgtatcttaa tttcttgcga ctgaacacta
     4741 aatactgaac actgaatact aatttcttca atatcacctg aaacttcatc atcacacaaa
     4801 ttgagatatt gaatttcaag ctgattccct tttagcaaaa ataatttctt gggttgaaaa
     4861 aaaaataaat ccggaaaatt caatccatca aaattattgg aatccaaaac ctctacatta
     4921 ttttttaaat cataagacaa atagccaaaa agccagtctt tggttgcttg ctgatattgt
     4981 tttaaatcct cgaaggcact gtgataatcc gttttaattg aagtaaaagc atctaccgca
     5041 agtaaacaat catagcttga atagttttgt ggataatcat tgctatccaa aaaaacaact
     5101 tccctaaatt gttgtgacca tgtcaacaaa tgttgcttga atagcgctgg attctcgata
     5161 tgttttataa ttgatgttct caagaaatga aatatttaaa aaattcaaaa atacgacaaa
     5221 tttattttaa attttttgct atatttatag tactattaac cctaaaacca gttaaatatg
     5281 aaaattttaa ccattattat cagaactgca attggtcttt tactattttc tgtggctatt
     5341 ggctattttt taaaactaac gccagaacca gtcacaaacg gaaattttaa agcatttgaa
     5401 attgggtttg tttcctcaga atacttaata ccaattgtaa aatttgtcac attactttgt
     5461 ggactggcat ttgtttttga tcgttacata actattgcaa gtcttctaat tttgcctttt
     5521 accataaacg ccttgtttct tgattattat ctgacaccac aagccttacc tgtctccatt
     5581 tttgtttttt taggaaactt tttcatcatt tatttacatc gaaaaaatta caaaaagtta
     5641 tttaaagtga aataacaaca aaagaaaccc gactaacaca tgtcgggttt cttttgatat
     5701 catatctccc ccaaaaaaaa gcagaaaatt attcttccaa attcaattta ttattaagaa
     5761 taaaattata tttactgtca tttttcacct gttcaaacca cacttttaaa ttgaattttt
     5821 atgattaaaa cttccaaaac aatactggct ttgcttattg ttattttcgc tatttcttgt
     5881 aaaaaagctg aagaagcaag cgaaagtgca tacgaatcta atacaattgc agttgacaca
     5941 acaaatacaa tctcttcatc agctgctgtt gaacctaaaa acagtgatcg aaaatttgtt
     6001 agaacagcag acattaaatt taaagtgaaa aatgtagcta attctactta tgcaattgaa
     6061 aataccgcca acaaatttgg aggttttgta acctatacca atttacaaag tactgtttct
     6121 gataaacaag aaaccaaaat aagtcaagac agtacacttg aaatcacaaa atacactgtc
     6181 gaaaacaata taactattcg ggttcccaac atccgtttgg atactgtaat taagactatc
     6241 gcaaaacaaa ttgattttct ggaatatcga attataaagg cagatgatgt ttcattgcaa
     6301 atgttgtcca atcaaatgca gcaaaataga agttctaatc aagaaaaaag attagaaaaa
     6361 gcaattgata caaaaggaaa aaaacttaat gaagttattg cagccgaaga taatttaaac
     6421 actaaaaatg aacaaaatga caacgctaaa cttgaaaatc tttccttgaa agatcaagta
     6481 aactttagta cattgacttt acaaatttac caaagagaaa ctattaagga agaaatggtt
     6541 gccaatccaa aaacatatag acaaggtttt ggctccaaaa ttcttgatgg aataatatca
     6601 ggctggtatg taatagaagg aattattgct gttatagctc agctttggtc tgttattttg
     6661 attggaattg gaggttatat tatattcaaa aaatatttcc ataaagggaa ttaaaccaaa
     6721 aaccccgttc agttataggc tgaacggggt ttctttgttg aacatgattt atacaattac
     6781 acgtgcaaag ctctattttc tgtagcagct aaagccgctt cttttatagc ttccgagaaa
     6841 gttggatgag cgtgactcat tcttgaaata tcttcggcag acgctttgaa ttccattgca
     6901 gtaactgctt cggcaattaa gtcggcacaa cgagctccaa tcatatgaat acctaaaact
     6961 tcgtctgttt ttttatcagc aagaattttc acaaaaccat ctaaatctcc acttgctctt
     7021 gctcttccca atgctttgaa agggaaactt cctgatttga attccactcc agaggctttt
     7081 aattgctctt ctgtttgtcc tactgcagca acttctggcc aagtgtaaac cactcctgga
     7141 attaaattat aatcgatatg tggtttttgt cccgccaaga tttcggcaac cattgtccct
     7201 tcttcttcag ctttgtgagc caacattgcg ccacgaacta catcgccaat ggcgtaaata
     7261 tttggaacat tggtttgcaa atggtcgttc acttctacca ttcctcgttc tgtaattttt
     7321 actccagctt tatcagcatt caatccatct gtataagggc gacgacctac agaaactaat
     7381 gaataatctc cttctaaagt aatcgtttct ccttttgcat tttcagcttg aacggttaca
     7441 ttgtctccat ttctttccac agatttaact ttgtgagaaa cgtagaattt cattccttgt
     7501 tttttcaata ctttagtcaa ttctttagac aaagcaccat ccattccagg aataattttg
     7561 tccataaatt ctactacgga aacttgtgct cccaatctca aatatacttg acctaattcg
     7621 attccgataa ctcctccacc aatga
//
LOCUS       DAOSNN010000118         7599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9658_length_7599_cov_4.708908, whole genome shotgun sequence.
ACCESSION   DAOSNN010000118 DAOSNN010000000
VERSION     DAOSNN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9658_length_7599_cov_4.708908"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PLQ70_07135"
     CDS             <1..925
                     /locus_tag="PLQ70_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQE34429.1"
                     /translation="NFSEEDFRGEQFKDFPHSLKGNNDLLSLTQPQAIKAVHALYFEA
                     GADIVETNTFSGTTIGMADYHLEEYVYQLNYESAKLAREVADEFTAKNPAKPRFVAGS
                     IGPTNRTASMSPDVNDPGYRAVTFDDLRIAYKQQVEALMDGGCDLLLVETIFDTLNAK
                     AALFAIEQVKEERNIDIPIMVSGTITDASGRTLSGQTVEAFLISVSHIPLLSVGFNCA
                     LGADLLKPYLQTLSHNTSFNISAHPNAGLPNAFGEYDETPEQMQAQIRSYLEDNLVNI
                     IGGCCGTTPEHIKLIADIAKDYKPRVSTAVM"
     gene            951..1454
                     /locus_tag="PLQ70_07140"
     CDS             951..1454
                     /locus_tag="PLQ70_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34430.1"
                     /translation="MKIVLSVFVLLCLFGFNDEKKSLMIQQEVPADFNFTIDSGGNDS
                     YNSEYNSFYRKYLNEDKTIKVELVREEKHKIYLFFKKVKFFEMPIKFVPTGRGNIKSQ
                     TPSFKKSILVYVNGKKKYVSYDNGETNDVNDKKAKSFLDLYEMIWNILYKKKEIIELP
                     KSDFHYK"
     gene            1637..4318
                     /gene="metH"
                     /locus_tag="PLQ70_07145"
     CDS             1637..4318
                     /gene="metH"
                     /locus_tag="PLQ70_07145"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HQE34431.1"
                     /translation="MAEAEKRRNLVLSGLEPLIITPNSVFVNVGERTNVTGSRKFLRL
                     IKEEKYDEALSIAKEQVEGGAQIIDINMDEGMLDGEYAMVKFLNLIAAEPDIARVPIM
                     IDSSKWHIIEAGLKVAQGKCVVNSISLKEGEEQFIHHAQLIKRYGAAAIIMAFDEVGQ
                     ADNYERRVEICQRSYDILVNKVGFPPQDIIFDLNIFPVATGMEEHRLNALDFFRGTKW
                     VRENLPHAHISGGVSNVSFSFRGNDVVREAMHSVFLYHAIKNGMTMGIVNPEMLSIYD
                     EIPKDLLEHVEDVILNRRDDATERLLDFAENVKGGPKSTEKEIQEWRLGTVQERITHS
                     LVKGVDAFIEEDVEEARLAATKPIEVIEINLMAGMNVVGDLFGSGKMFLPQVVKSARV
                     MKKAVAYLLPFIEAAKQVGDKEGNGKILMATVKGDVHDIGKNIVSVVLACNNYEIIDL
                     GVMVPPEKIIAAAVEHKVDIIGLSGLITPSLDEMVYLAKELDKRNMDIPVMIGGATTS
                     RAHTAVKIAPQYKGTVIHVNDASRAVTVAGNLLDDNREQYTSDIRTDYDAFRESFLGR
                     SRDKNFLSIEDARKNKLQLDWANFTPVKPNVIGEQVIEVDLDVLVPYIDWTPFFQTWE
                     LHGKYPAILTDEVVGEQATSVFADAQAMLQVILKEKKLQAKGIYGIFPANQVNDDDIQ
                     LYDENGKELEKFLTLRQQSQKTKGAPNIALADFIAPKDNGVTDYMGAFCVTTGFGVDE
                     WADEYRDNLDDYNSIMVKALADRFAEAFAEYLHEKVRKESWGYDSDETLSKEDLIKEN
                     YKGIRPAPGYPACPDHLEKPTIWKLLDVENKIGVTLTESMAMWPASSVSGYYFGNPES
                     KYFGLGKIKEDQVIDYAKRRSISTDMAMKWLNPNIAD"
     gene            4416..4772
                     /locus_tag="PLQ70_07150"
     CDS             4416..4772
                     /locus_tag="PLQ70_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQE34432.1"
                     /translation="MEYNKLEVWLEARKLVNLLYGSSKLFPKEELFGLTNQMRRAAIS
                     ILSNIAEGCGRQTSKDTLHFLHISRGSLYELETQYYLALDQKYIDSTNFNIAFEQIQS
                     CKKLLNGFINYYKSKI"
     gene            4852..>5519
                     /locus_tag="PLQ70_07155"
     CDS             4852..>5519
                     /locus_tag="PLQ70_07155"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQE34433.1"
                     /translation="MKVTQHIEDAKGKPLFSFEIVPPQKGTNINDLYVNIDPLMEFKP
                     PFIDVTTSREEYVYIEKDGLFDRRITRMRPGTVGICASIQHKYGVDAIPHVLCGGFTK
                     EETEYLLVDCQYLGIDNLVALRGDPMKGEKYFEPTKGGNAYAVDLVKQIKAMNAGQFL
                     HDTLPMQNAPDFCVGVAGYPEKHIEAPSLEADLKRLKEKVDAGADYIVTQMFFDNQRY
                     FKFV"
     assembly_gap    5520..5619
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5757..6158)
                     /locus_tag="PLQ70_07160"
     CDS             complement(5757..6158)
                     /locus_tag="PLQ70_07160"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQE34434.1"
                     /translation="MKNFLLLVVLFFSIQLGYGQEKITHPNQNSLYNSAGISVKPEFP
                     GGQKEFEKYIRKNYITPREAKEIKGKVYVTFIIEIDGSLTDIKVLRDLGFGTGKEAIR
                     VLEQSPKWIPGQQKGLIVRTTYSLPISINGF"
     gene            complement(6284..7180)
                     /gene="gldA"
                     /locus_tag="PLQ70_07165"
     CDS             complement(6284..7180)
                     /gene="gldA"
                     /locus_tag="PLQ70_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HQE34435.1"
                     /translation="MSIEVNNISKSYGVQKALDNVSFSIQKGEIVGFLGPNGAGKSTL
                     MKILTTYIAADEGLATVNGFDVNSQSKSVQQSIGYLPEHNPLYLDLYVREYLAFNADV
                     YKVAKPRIEEVIQLTGLSNESHKKIGQLSKGYRQRVGLANALLHNPDVLILDEPTTGL
                     DPNQLVEIRNVIKNVGKDKTVFLSTHIMQEVEAICDRVIIIDKGKIVADKKLDKLIST
                     TTEQVIEVEFDKTVDFALIARIENLVSYKNTQDSIWELTFNADKDMRPAVFDFANANG
                     LRTLQLNQKNKNLETVFREMTK"
BASE COUNT         2440 a         1184 c         1547 g         2328 t
ORIGIN      
        1 taatttctct gaagaagatt tccgaggaga acaattcaag gattttccac attcgctaaa
       61 ggggaacaat gatttattgt ccttgacgca accgcaagcc atcaaagccg tgcacgcttt
      121 gtattttgaa gcgggagccg atatcgtgga aaccaatacg ttttccggaa ctactatcgg
      181 aatggcggat tatcatttgg aagaatacgt ttatcaattg aactacgaat cggcaaaatt
      241 agctcgtgaa gtagccgatg aattcacggc caaaaatcca gccaaacctc gttttgtagc
      301 gggttccatt gggccgacaa acagaacagc aagtatgtca ccagacgtga acgatccggg
      361 ttaccgtgcc gtaacttttg atgatttgcg cattgcctac aaacaacaag tggaagcctt
      421 aatggacgga ggttgcgatt tgcttttggt agaaactatt tttgatacct tgaatgccaa
      481 agcagcactt ttcgctatcg aacaagtgaa agaagaacgt aatatcgata ttccaattat
      541 ggtttcagga acgattaccg atgcttcagg aagaacactt tcaggacaaa cggtagaagc
      601 tttcttgatt tcggtttcac atattccgtt gttgagcgtt ggtttcaatt gcgctttagg
      661 agctgatttg ttgaaaccgt atttgcagac tttgtcacac aatacatcgt tcaatatatc
      721 tgcgcatcca aatgcaggat tgccaaatgc ctttggagaa tacgacgaaa ctccagagca
      781 aatgcaagca caaatacgaa gctacttgga agataattta gtaaacatca taggtggttg
      841 ttgcggaaca accccggaac acatcaagtt gattgctgat attgcgaagg attacaagcc
      901 gagggtttcg actgcggtga tgtaattaat ttttttggaa aatgagacga ttgaaaatag
      961 tattatcagt ttttgtgctt ttgtgcttat ttgggtttaa tgatgaaaaa aagtcattaa
     1021 tgatccagca agaagtacca gctgatttta attttacaat tgatagcgga gggaatgata
     1081 gttacaattc cgaatataac agtttttatc ggaaatattt aaatgaagat aagacgataa
     1141 aagttgaatt agtaagagag gaaaagcata aaatttattt attttttaaa aaggttaaat
     1201 tttttgaaat gccaataaaa tttgtaccta caggaagagg gaatattaaa agccaaactc
     1261 caagttttaa aaaaagtatt ttggtttatg taaatgggaa aaagaaatat gtaagttatg
     1321 acaatggtga gacaaatgat gtaaatgata aaaaagcaaa atcttttttg gatttgtacg
     1381 aaatgatttg gaatatatta tataaaaaga aagagattat agaattacca aaatcagatt
     1441 ttcattataa ataattagat aactactgct agcgcgagct tctagctcgt gcccacaaaa
     1501 aaaggcagac aaaatagtag aaaaataaaa taggcacgag cgtgacgctc gcgttagcaa
     1561 cataagagtt ccgtaggaac gacatatttt gtagcaacgg aatttattcc gttgttgtaa
     1621 ataaaaaaat aaaaaaatgg cagaagcaga aaaaagaaga aatttggttt tatcgggatt
     1681 agaaccctta atcattacgc caaacagcgt atttgttaac gttggtgaac gtaccaacgt
     1741 gaccggttcg agaaaatttc ttcgattaat caaggaagag aaatacgatg aggccttaag
     1801 cattgccaaa gagcaagtgg aaggtggcgc gcaaatcatc gacattaata tggatgaggg
     1861 gatgctggat ggcgaatatg cgatggtgaa attcctgaac ctgattgctg ccgaaccgga
     1921 tattgcccgt gtgccgatta tgatcgacag ttcgaaatgg catattatcg aagccggttt
     1981 gaaagtggct caaggaaaat gtgtggtgaa ttcaatctct ttgaaagaag gtgaagagca
     2041 attcatccat cacgcccaac tgattaaacg ctacggagca gcagccatca ttatggcttt
     2101 tgacgaagtg ggacaagccg ataattacga acgtagagta gaaatttgcc aacgctcgta
     2161 tgatattttg gtcaacaaag tgggctttcc tccacaggat attattttcg atttgaatat
     2221 atttccggta gcaacaggaa tggaagaaca ccgcctgaat gctttggatt tctttagggg
     2281 aaccaaatgg gttcgcgaaa atttgccaca tgcacacatc agtggtggtg tgagtaacgt
     2341 ttcgttttcg tttagaggaa atgatgtggt gagagaagcg atgcactcgg ttttcttgta
     2401 ccacgccatt aaaaatggaa tgacgatggg aattgtaaac ccggaaatgc tttctattta
     2461 tgatgaaatt ccgaaagatt tattggaaca cgtggaggat gtaattctga accgtcgtga
     2521 cgatgccact gaaagactgt tagattttgc cgagaatgta aaaggaggtc caaaaagcac
     2581 ggaaaaagaa attcaggaat ggcgtttggg aaccgttcaa gaaagaatta cgcattcact
     2641 tgtaaaaggt gttgatgcct ttattgaaga ggatgttgaa gaagctagac tagcagcaac
     2701 caaaccaatc gaagtaatcg aaatcaattt gatggcggga atgaatgtcg ttggagattt
     2761 gtttggttct gggaaaatgt ttttgccaca ggtggtaaaa tcagctaggg ttatgaaaaa
     2821 agcagtggct tatctgttgc cattcatcga agcggcgaaa caagtgggcg acaaagaagg
     2881 taacggaaaa atattgatgg caaccgtaaa aggagatgtt cacgatattg gtaaaaatat
     2941 tgtttcggtg gtgttggctt gtaataacta cgaaatcatt gaccttggtg taatggttcc
     3001 tcccgagaaa attatcgcgg cagctgtcga acataaagta gatattattg gtttgagtgg
     3061 attaatcacg ccatccttgg acgaaatggt gtatctcgcc aaagaattgg ataagcgtaa
     3121 tatggatatt ccggtgatga ttggtggagc aacgacttcg cgtgcgcata ctgccgtaaa
     3181 aattgcacct caatataaag gaacggtcat tcacgtaaac gatgcttcga gagcggtaac
     3241 cgtggccgga aatttattgg acgataatag agaacaatat acttccgata tcagaaccga
     3301 ttacgatgct ttcagggagt cgttcttggg acgttccaga gacaagaatt tcttgagtat
     3361 tgaagatgcc cgtaaaaata aattgcaatt ggattgggct aattttactc ctgtaaaacc
     3421 aaatgtaatt ggcgaacaag tgatcgaagt ggatttggat gttttggttc cttacatcga
     3481 ttggacgccg ttttttcaaa cttgggaatt gcacggaaaa tatccagcaa tcttaacaga
     3541 tgaagtggtt ggagaacaag caacttctgt ttttgcagat gcacaagcga tgttacaagt
     3601 gattctgaaa gaaaagaaat tacaagccaa aggaatttac ggcattttcc ctgcgaatca
     3661 agtcaatgat gatgatattc aattgtatga tgaaaacgga aaagaattgg agaaattctt
     3721 gaccttgcgt cagcaatccc aaaaaacaaa aggagctccg aatattgctt tagccgattt
     3781 tattgcgcca aaagacaacg gtgtaaccga ttatatggga gctttttgtg tgactacagg
     3841 ttttggtgtg gacgaatggg ctgatgaata ccgtgataat ttagacgatt acaattcgat
     3901 tatggtaaaa gcattagcag accgttttgc agaagctttc gccgaatatt tacacgaaaa
     3961 agtacgtaaa gaatcttggg gatatgattc ggacgaaact ttatcaaaag aagatttgat
     4021 taaagaaaat tacaaaggaa ttcgtccggc gcctggttat ccggcttgtc cagaccattt
     4081 agaaaaacca accatttgga aattacttga tgtggaaaat aaaattggag taacgttgac
     4141 agaaagtatg gcgatgtggc cggcttcttc ggtttctgga tattattttg gaaatccgga
     4201 aagcaaatat tttggactcg gaaaaataaa agaagaccaa gtaattgatt acgccaaaag
     4261 aagaagtatt tcaactgata tggcaatgaa atggttgaac ccaaacatag cagattagat
     4321 tttagttgtt ggttgatggt tgtcggttgt tgggttttcc aatcatcatc aagtttttcc
     4381 aatcaaccaa caacatcaac tataaacaaa aaaaaatgga gtataataaa ttagaggttt
     4441 ggttagaagc tagaaaacta gttaacttgt tgtacggttc atctaaatta tttccaaaag
     4501 aagagttgtt tggtttgacc aatcaaatga gaagagctgc aatttcaata ctttcaaata
     4561 ttgcagaagg ttgcggtcgt caaacatcta aggatacatt gcactttttg catatttcaa
     4621 gaggttcatt atatgaatta gaaactcaat attatttggc attagatcaa aaatatatag
     4681 attctactaa ttttaatatt gcttttgaac agattcagag ttgtaaaaaa ttattaaacg
     4741 gatttatcaa ttattataaa agtaaaatat agttaatggt tgttagttga ttgctgttgg
     4801 gattacactt ccgaccatca accaacaacc atcaactaac aaccaaaaca aatgaaagta
     4861 acacagcata tagaggacgc aaaagggaaa cctttgtttt ctttcgaaat tgttccgcct
     4921 caaaaaggga caaatatcaa tgatttatac gtaaatattg atccgttgat ggagtttaaa
     4981 ccgccgttta ttgatgtaac tacttcaaga gaagagtatg tttatatcga aaaagacgga
     5041 ctttttgatc gtcgaatcac tcgtatgcgt ccaggaacag taggaatttg tgcttccatc
     5101 caacataaat atggggttga tgccattccg cacgttttgt gtggtggttt taccaaagaa
     5161 gaaaccgagt atttattggt cgattgccaa tatttgggaa tcgataattt ggtggcactt
     5221 cgtggtgatc caatgaaagg cgaaaaatat tttgaaccta ccaaaggtgg aaatgcctat
     5281 gcggtggatt tggtaaaaca aatcaaggcg atgaatgccg gacagttttt gcatgatact
     5341 ttgccaatgc aaaacgcacc tgatttttgc gtgggtgtgg ctggttatcc cgaaaaacac
     5401 atcgaagcac caagtttgga agccgatttg aaaagattaa aagaaaaagt ggatgccgga
     5461 gccgattata tcgtaaccca aatgtttttt gacaatcaac gttatttcaa gtttgtcgan
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cagataatat tcatcaaatt
     5641 gcgagtcagg ttttttaaag caaataattc agaaaagctg ttttatataa aataaaaagc
     5701 atcgtttcca taaggagcga tgctttttta gttaattaag aaaacctatt ttaaaattaa
     5761 aatccattaa tagaaatcgg taaactataa gttgttctta ctattagtcc tttctgttgt
     5821 cctggaatcc attttggact ttgttctaaa actcgaattg cctcttttcc tgtgccaaag
     5881 cctaaatctc gtaaaacttt tatatcggta aggcttccat ctatttcaat gataaaagtt
     5941 acatatactt ttccttttat ttcttttgct tcacgagggg tgatgtagtt ttttctaatg
     6001 tatttttcga attctttttg acctccaggg aattctggtt ttacgcttat tccagccgaa
     6061 ttataaagag aattttgatt ggggtgtgtt attttttctt gtccgtagcc taattgtatg
     6121 gagaaaaata aaactactag caataaaaag tttttcattt gaaggggttg ttgaaagttt
     6181 tttttgtaaa tatattcttt attttgaata atttatcact tcatttgaaa ataagtatat
     6241 ttttttgttt gtaaactttg attgttatct gaaatctgcc aacttatttt gtcatttccc
     6301 taaaaacagt ctctagattt ttattttttt ggttaagttg taaagttctt aatccattag
     6361 cattggcaaa atcaaaaacg gccggacgca tgtctttatc ggcgttgaaa gtcaattccc
     6421 aaatagaatc ttgtgtgttt ttataggaaa ctagattttc gattctagcg attaatgcaa
     6481 aatcaacagt tttgtcaaat tctacttcga ttacttgttc ggtcgttgtg gaaatcaatt
     6541 tgtctagttt tttgtcggca acaatttttc ctttgtcaat gataatcact cggtcacaaa
     6601 tagcttctac ttcctgcata atatgcgtcg aaaggaaaac ggttttgtcc ttaccaacat
     6661 ttttaatcac atttctaatt tcaactaatt ggttggggtc taaacccgtt gttggttcat
     6721 ccaaaatcaa aacatcggga ttgtgcaata aagcgtttgc caatcccacg cgttggcggt
     6781 atcctttgga taattgtcct attttcttgt ggctctcatt ggataacccc gtcaattgaa
     6841 tcacttcttc aattctgggt ttagctactt tgtacacatc ggcattgaaa gccaaatatt
     6901 ctctcacgta caaatccaaa tacaacggat tgtgttccgg taaataaccg atagattgtt
     6961 gtacagattt ggattgcgaa ttaacatcaa atccatttac agttgctaaa ccttcatcgg
     7021 cagcgatata ggtcgttaat attttcatca aagtcgattt tcctgcgcca ttcggcccca
     7081 aaaaacctac gatttctcct ttttggattg aaaatgaaac gttgtctagt gctttttgga
     7141 ctccgtaact tttcgaaatg ttgtttactt ctattgacat cgctattata tttgaagcaa
     7201 aagtaaacag aatatttctt gatttattgt tttttaacta tacaatagac aaaaagactt
     7261 ggttgaaggt ttttgctatt gaaagttatt caaatttggt tgcttaattg ttattttaat
     7321 gattgatttt taattattta gtaataaaaa gcagtttttg tagaaaattc cttttttatt
     7381 gtgtgaaatt ggtctttttt ttgggttgac aaaaaaatat ttatacattt gcagggaatt
     7441 tatcaaaaag cagaaacaaa atgtcatcta accgttttta ttttagcaat acttactatt
     7501 tcttctttag gaagtaagga ttgtctatgg ttatttgaaa gattaagaag caaataaaac
     7561 taatatacaa tcctgatgaa aatcaggatt tttttttga
//
LOCUS       DAOSNN010000119         7579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9693_length_7579_cov_5.174242, whole genome shotgun sequence.
ACCESSION   DAOSNN010000119 DAOSNN010000000
VERSION     DAOSNN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9693_length_7579_cov_5.174242"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLQ70_07170"
     CDS             complement(<1..507)
                     /locus_tag="PLQ70_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQE34436.1"
                     /translation="MDFNLTEEQLMVQQAARDFAQNELLPGVIERDEKQIFPAEQIKK
                     MGELGFMGMMVDPKYGGSGLDTISYVLAMEEISKIDASASVVMSVNNSLVCWGLQEFG
                     TEEQKQHWLPLLASGQIHGAFCLSEPEAGSDATSQKTTAIDMGDYYLVNGTKNWITNG
                     NTASFYIVI"
     gene            604..1692
                     /locus_tag="PLQ70_07175"
     CDS             604..1692
                     /locus_tag="PLQ70_07175"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013073324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HQE34437.1"
                     /translation="MKKNYYNVIGLMSGTSLDGVDLSHIKFQLIDTKWTFEILESETI
                     SYSQDWIDQLKTAVDFSETQLEKLNHDYTKLLASIISNFIEKHKIENLDAVCSHGHTI
                     LHQPQNGLTLQIGNLPEIATLTHQTVVCDFRVQDVKLGGQGAPLVPIGDRILFSDYDY
                     CINLGGFSNISFEQNSKRIAFDISPVNTVLNFYANQLGLNYDDKGQISRTGKVNTNLL
                     NELNALDFYQQTFPKSLGFEFVKETVLPLIESYSIAVEDKLCTFTEHVALQTALALDL
                     FETRKDKKNRMLVTGGGAYNDFLIERIQSQLPEIEIIIPSSKILEFKEALIFALLGVL
                     KLRGEINVLSSVTGAKADHSSGEIYSFT"
     gene            1706..2860
                     /gene="nhaA"
                     /locus_tag="PLQ70_07180"
     CDS             1706..2860
                     /gene="nhaA"
                     /locus_tag="PLQ70_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HQE34438.1"
                     /translation="MIQKTRNISSKLFKEFLESEKAGGLILIACTLISLALANSIFSS
                     EYIHFWHTSFHGQPIEYWINDGLMTIFFLLIGLELEREVYQGELSNIKNALLPIFGAI
                     GGMLIPAAIYLYFNYGTSTQSGAGIPMATDIAFALGILSLLGNKVPTSLKIFLTALAV
                     IDDLGAILVIALFYTKTIFWTNLSIAMGIFVVLLILNRIKVRNLIPYLTLGVVMWYFM
                     LNSGVHATITGVLVAFAIPFGNGDKKSTSYILQHFLHKPVAFIILPVFALANTAIILD
                     LEIQEIVTQHYSIGIALGLIVGKPLGIFILSLIAVKLGICKLPSDLNWTSIFGVGFLG
                     GIGFTMSIFVTLLAFEDVTIINHAKIIILLSSLLAGIIGFVGLKFVLKTD"
     gene            2935..3831
                     /locus_tag="PLQ70_07185"
     CDS             2935..3831
                     /locus_tag="PLQ70_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQE34439.1"
                     /translation="MSTTISSDQRKSILISVLLYGLLLLILFFIRFWPPANLEELTGG
                     GGGGGVTVNFGDSDLGSGANYKSEILDVHNQAKQTPTQTTPDESILSQENTTEESVVI
                     PQKEKPLKPTPVVKEEPKPVVVAKPKVSNNTNDALASIMKGSNKGGDGDDKTAGNKGK
                     ANGSLSSNGYYGTGGSGGGTGGGNGTGNGIGTGSGYGAGSGGGSGNGSGGGYSLGNRK
                     AISKPAPKYTCNESGKVVVEVSVDRNGRTISAIAGIKGSTNTASCLLEQAKIAAMNTR
                     WDASSDAPEKQVGKIVYNFNLN"
     gene            complement(3925..4320)
                     /locus_tag="PLQ70_07190"
     CDS             complement(3925..4320)
                     /locus_tag="PLQ70_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQE34440.1"
                     /translation="MSIKRKRRFHAEVATSSLSDIMFFLLLFFLIISTLANPNVIKMT
                     LPKAKSNEKTNKQYISLSVTEDKKFYLDKQPVPFEELETTLMSKMNTEKDQTVIVRIP
                     FNLQVQDLVDVLQIGVKNNLKFVIATSPK"
     gene            complement(4325..5020)
                     /locus_tag="PLQ70_07195"
     CDS             complement(4325..5020)
                     /locus_tag="PLQ70_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQE34441.1"
                     /translation="MFSLIQLQADTIANAATGAVVENIAPNTEISVLQFIFKGGFFLI
                     PIAILLFYTFYLVIERYLYIQKASKIDSLMMLDVRDNLNSGKLDRALSIVERNNTSSG
                     NVIKEGILLIGRPISEIESNMDRAADIEIGEMEKHLGQLGLIAGIAPTLGFIGTISGV
                     IKIFYSISVTENISIGNISGGLYEKMISSGSGLIVGIIAYSAYHLLNGKIDNFALKIQ
                     KQILEFVNIIQKS"
     gene            5145..6386
                     /locus_tag="PLQ70_07200"
     CDS             5145..6386
                     /locus_tag="PLQ70_07200"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HQE34442.1"
                     /translation="MNYQETINWMFNQLPMYQLQGASAYKKDLTNAHLLSNHLDNPEK
                     KLKCIHVAGTNGKGSTSHMLASILQEAGYTVGLYTSPHLKDYRERIKINGEEISEDFV
                     CEFITKNKAFFEANDISFFEMTVGLAFDYFAKEKVDIAIIEVGMGGRLDATNIITPLV
                     SVITNIGLDHTQFLGNTLEAIAFEKAGIIKPAIPVVIGEYTPETKPVFLSKANENHSE
                     IYFASDLISHNYPSDLIGDYQKHNKKTVLQTIAVINSQKEFKIYETDIKSGLLNVVHN
                     TGLEGRWQQLGDSPKIICDTAHNKNGLEIVMKQIQKEKFDTLHIVLGVVNDKDLDEVL
                     PLFPTNAIYYFCKPNIPRGLETNVLQQAALKHNLIGDTYPSVASAYSQSKKNATGNDF
                     IFIGGSTFVVAELPINKRISL"
     gene            6498..7298
                     /locus_tag="PLQ70_07205"
     CDS             6498..7298
                     /locus_tag="PLQ70_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34443.1"
                     /translation="MKKSSFLASVMLILTFILSTTICAQEEQKPVFITVTTMHRNLNA
                     DGKDWRKTEQEYYDKVTSKNDLIIGSEILTHYYTANSTEILHISVYKTWEDIEKSADV
                     SDELIKKAWPDEKARKEFFDKQRSYYTTMHSDEIYTSSPLAGHKELKTDSKEPMIVYI
                     RKSQLSMTGQGKGLKEFNEKVTLKNPYIKAYYPHRHYWGADSRDFVEAFLFDSMSDME
                     KSSDKDDELIKVAWPKEEDRKAFFDEMRKSFTGLHGDFIYHNEPTMSK"
     gene            7504..7578
                     /locus_tag="PLQ70_07210"
     tRNA            7504..7578
                     /locus_tag="PLQ70_07210"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7539..7541,aa:Val,seq:tac)
BASE COUNT         2594 a         1312 c         1311 g         2362 t
ORIGIN      
        1 aatgacaata taaaacgaag ctgtattccc gttagtaatc caattttttg ttccgtttac
       61 caaataataa tcgcccatat cgatagcggt tgttttttgt gaagtggcat cgctaccagc
      121 ttcgggttcg ctcaaacaaa aagcaccgtg aatttgaccc gatgccaaaa gtggcaacca
      181 atgttgtttt tgttcttcgg ttccaaattc ctgcaatccc caacaaacaa gagaattatt
      241 gacagacatc accaccgaag ctgaagcatc aattttagaa atttcttcca ttgccaaaac
      301 ataggaaatc gtatcgagac cgcttccacc atattttgga tcaaccatca tccccataaa
      361 accgagctct cccatttttt tgatttgttc agctggaaaa atttgctttt cgtctctttc
      421 aattacacca ggcaaaagtt cgttttgggc aaaatctctg gcagcttgtt gcaccattaa
      481 ttgctcttcg gttagattaa aatccataat tatttaaaat aaatgacgtt atttgttata
      541 aaaatacatt caaagataat tttttatacg gaactattca attatgattc gtaaatttga
      601 ctaatgaaaa aaaattacta caacgttatc ggattaatgt caggaacctc gctggatgga
      661 gttgacttat ctcatattaa attccaatta atcgatacca aatggacttt tgaaatccta
      721 gaaagtgaaa ccattagcta tagccaagac tggattgacc aactcaaaac agcagtagat
      781 ttttcagaaa cacagcttga gaaactaaac cacgattaca ctaaacttct cgcttctatt
      841 atttctaatt ttattgaaaa acataaaatt gaaaatctgg atgccgtttg ctctcacggt
      901 cacactattt tgcatcaacc ccaaaatgga ttgacgctac aaattggcaa tttaccagaa
      961 attgcgactt tgactcatca aacggtggtt tgtgatttta gggttcaaga cgtcaaattg
     1021 ggtggtcaag gcgctccttt agttcctatt ggtgaccgaa ttttattttc ggactatgat
     1081 tattgtatta atttgggtgg tttttcaaac atctctttcg aacaaaacag caaacgaatt
     1141 gcttttgata tttcgcctgt aaatactgtt ctgaattttt atgccaatca attggggttg
     1201 aattatgatg acaaaggcca aatttcacga acaggaaaag tcaataccaa tttgttaaac
     1261 gaattaaatg ccttggattt ttaccaacaa accttcccta aatcattggg ttttgaattt
     1321 gtaaaagaaa ctgttctgcc tttaattgaa agttactcaa ttgctgttga agacaaatta
     1381 tgtactttta cagagcatgt ggctttacaa actgctttgg ctttggatct cttcgaaact
     1441 cgcaaagaca agaaaaatcg aatgttagta actggcggag gtgcttataa tgattttcta
     1501 atagagagaa ttcaatctca attacctgaa attgaaatta ttattccgtc atcaaaaata
     1561 ttagaattca aagaagcttt aatatttgcc ttgcttggcg tcttgaaatt gcgaggcgaa
     1621 attaatgttt tgagcagtgt aacaggtgca aaagcagatc atagttcggg agaaatttat
     1681 tcctttactt aacaactttt aaaaaatgat acaaaaaacc cgaaacatat cttcaaaatt
     1741 attcaaagaa tttttagaaa gtgaaaaagc agggggactt atcctaatag cctgcacatt
     1801 gatttcattg gcattggcga attctatttt tagttcagaa tatatccatt tttggcacac
     1861 ctcttttcat ggtcaaccta tagaatattg gattaatgat ggattaatga ccattttctt
     1921 tttgctgatt ggtttagaat tagaaagaga agtctatcag ggagaattgt cgaatatcaa
     1981 aaatgcgctt ttacctattt ttggagccat tggaggaatg cttattccag cagccatcta
     2041 tttatatttt aattatggta cttcaactca atctggagca ggaatcccga tggcaaccga
     2101 tattgctttt gccttaggaa tcttatcctt attaggcaat aaagttccaa cttcactaaa
     2161 aatattcctg actgcattgg cggttattga tgacttggga gcgattttag taattgcttt
     2221 attttacact aaaactattt tttggacaaa tctttccatt gcaatgggca tttttgttgt
     2281 attgttaatt ttaaaccgaa tcaaagtaag aaacctaatt ccttatctga ctttgggagt
     2341 cgtgatgtgg tattttatgc ttaattctgg tgtgcacgca acaattactg gagttttagt
     2401 agcttttgca atcccttttg gaaatggaga taaaaaatct acatcctata tattacaaca
     2461 ttttttgcac aaaccagtcg catttatcat acttccagtt tttgcattgg ctaatacagc
     2521 catcattttg gatttggaaa tacaagaaat tgtaacccag cattacagta tcggaattgc
     2581 attgggactt attgttggaa aaccccttgg aatttttata ttaagtttga ttgcagtgaa
     2641 attaggtatt tgcaaacttc ctagtgacct gaattggact tctatatttg gcgtaggatt
     2701 cttgggcgga attggattta caatgtctat ttttgtgacc ttattagctt ttgaagatgt
     2761 aactataatc aatcatgcca aaattataat tctactttct tctttactag ctggaataat
     2821 tggatttgtc ggtttgaaat ttgtattaaa aacggattaa taccatttta gtaagcaagc
     2881 aatttttagt acatttcaac gtttttaaaa ccaatacgca ttaacacatt agcaatgagc
     2941 acgaccattt cttccgatca aagaaaatca atactaatct ccgttttatt atacggactc
     3001 ttgctgttga ttttattctt cattcggttt tggcctccag ctaatctgga agaattgact
     3061 ggtggcggtg gtggcggagg cgttaccgtg aattttggcg acagcgattt gggttctgga
     3121 gccaattata aaagcgagat tttggacgtt cacaatcaag ccaaacaaac tccaactcaa
     3181 actactccag acgaatccat cttgtcacag gaaaatacca cagaggaaag tgtcgtaatt
     3241 ccgcaaaaag aaaagcctct aaaaccaacg cctgtagtaa aagaagaacc gaaacctgtt
     3301 gtagtagcca aaccaaaagt ttcaaataat actaatgatg ctttggcaag tattatgaaa
     3361 ggctcgaata aaggtggtga tggcgatgat aaaacagctg gcaacaaagg aaaagccaac
     3421 ggaagtttaa gttctaatgg ctattatgga actggaggat ctggaggcgg aactggcgga
     3481 ggtaacggaa ccggcaacgg aattggaaca ggaagcggat acggtgctgg aagtggtgga
     3541 ggttctggaa atggaagtgg cggaggttat tcattaggaa atagaaaagc aatttctaaa
     3601 cctgctccaa aatacacttg caacgaatca ggaaaagtcg ttgtagaagt ttccgtggat
     3661 agaaatggga gaacaataag tgcaattgcg ggaataaaag gctccaccaa tacagcaagt
     3721 tgtttactag aacaagctaa aattgctgca atgaatacac gatgggatgc cagcagcgat
     3781 gcaccggaaa aacaagtggg taagattgtt tataatttca atttgaatta agaatagaga
     3841 tgaatttata tagataaata aataaaaaat gcctcggaat gaattcgagg catttttgtt
     3901 tgatagtttt atagaaaatc aatactattt cggactagta gcaatcacaa atttcagatt
     3961 gttttttacc ccaatttgta aaacatcaac taaatcttga acttgtaaat tgaacggaat
     4021 tctaacaata acggtttggt ctttttcggt attcatttta gacattaaag ttgtttctaa
     4081 ttcttcgaaa ggaacaggct gcttgtcgag atagaatttc ttatcttcgg taacggataa
     4141 actaatgtat tgtttgttcg ttttctcgtt agatttggct tttggcaatg tcattttaat
     4201 cacattcgga ttcgccaaag tggatataat caagaaaaaa agcaataaga aaaacataat
     4261 atcactcaac gaagaggtag caacctcggc gtggaatctt ctttttcgtt ttatggacat
     4321 tgcgctatga tttttgaata atatttacaa attctaatat ttgcttttgg attttcagcg
     4381 cgaaattatc aatcttaccg ttcaataagt gataagcact gtaagcaata atccctacta
     4441 tcaacccaga accactacta atcatttttt cgtacaatcc gcctgagata ttaccaatac
     4501 tgatattttc ggttactgaa atgctgtaaa atatcttgat aactcccgaa attgttccga
     4561 taaatcctaa ggttggagca attcctgcaa tcaatcctaa ctgtcctaaa tgtttttcca
     4621 tttcgccaat ttcaatatcg gcagcacgat ccatattcga ttcaatttca gaaataggtc
     4681 tgcctattaa taaaatccct tcttttataa catttccaga agaagtatta tttctttcta
     4741 cgatagacaa tgccctatcc aactttccag agttgagatt atctctcaca tctagcatca
     4801 ttagagaatc aattttagac gctttttgaa tgtataaata acgttcgatt actaaataaa
     4861 atgtataaaa taagagaatt gcaattggaa ttaagaaaaa acctccttta aaaataaatt
     4921 gtaaaaccga gatttctgtg ttaggagcga tattttcgac tactgcacca gtagcagcat
     4981 tggcaatagt atcggcttgt aattggatta aactaaacat aaattattat tttattcatt
     5041 aattctaaaa aatacttgaa atttgcaacc aaagatactt ttcgatgtac aattaaacta
     5101 aaaaaaacga aaattatttt agtccacctc ctatttaaca gaaaatgaat tatcaagaaa
     5161 ccataaactg gatgtttaat cagttgccaa tgtaccaact tcagggcgca tcggcttaca
     5221 aaaaagattt gacaaatgct catttgcttt cgaatcatct tgataatcct gaaaaaaaac
     5281 taaaatgtat tcacgttgca ggaaccaacg gcaaaggttc tacatcgcat atgttggcat
     5341 cgatacttca ggaagctggt tacaccgttg ggttatacac ttcgccacat ttaaaagatt
     5401 acagagaacg catcaaaatt aacggggagg aaatctcgga agattttgta tgtgaattca
     5461 taaccaaaaa caaagctttt tttgaagcca atgatataag tttctttgaa atgactgttg
     5521 gtttggcttt cgattatttt gccaaagaaa aagtggacat cgccattatc gaggtgggaa
     5581 tgggtggaag attagatgcc accaatatca ttacaccact ggtttcagta attacaaata
     5641 ttggtcttga tcatactcaa tttctaggca ataccctaga agcgattgct tttgaaaaag
     5701 caggaatcat caaacctgca attcctgttg ttattggaga atacactccc gaaactaagc
     5761 ctgttttttt gtccaaagcc aatgaaaatc attctgaaat atactttgct tctgacttga
     5821 tttcgcataa ttatccatca gatttgattg gagattatca aaaacacaac aaaaaaacgg
     5881 ttttgcaaac cattgccgta atcaactccc aaaaggaatt taaaatctat gaaaccgaca
     5941 ttaaatcggg tttattgaat gttgtacaca atacaggact cgaagggcga tggcaacaac
     6001 taggcgattc accaaaaata atttgcgata cagcacacaa caaaaatgga ttggaaatcg
     6061 tgatgaaaca aatccaaaaa gaaaaattcg acaccttgca cattgttttg ggagtagtaa
     6121 acgacaaaga tttggatgaa gttttacccc tattccctac taatgcaatt tattatttct
     6181 gtaaacccaa tattcctaga ggcttagaaa caaatgtttt acaacaagct gcgttaaaac
     6241 acaatcttat tggagacaca tatccttctg ttgcaagcgc ttacagccaa tctaagaaaa
     6301 atgccacggg taatgatttt attttcattg gtggaagtac ctttgttgtc gctgaattgc
     6361 caataaacaa aaggatttca ctttaataca cctttttttt ataaaaaaat agtttaattt
     6421 aatctaaata aatgattatt aattgattag ctttgtgttc taattttcaa ttaattaacc
     6481 atttaaacta gattattatg aaaaaaagta gttttttagc atcggtaatg ttaattttaa
     6541 catttatttt gagtacaaca atttgtgctc aagaagaaca aaaacctgta ttcattacag
     6601 taacaactat gcatcgaaat cttaatgcag acggaaaaga ttggcgaaaa acagaacagg
     6661 aatattatga caaggttaca agtaagaatg atttaatcat tggttccgaa attctgacgc
     6721 attattatac tgccaacagt actgaaattt tacatatttc agtttacaaa acctgggaag
     6781 acattgaaaa atcagcagac gtttcagacg aattaataaa aaaagcttgg ccagacgaaa
     6841 aggctagaaa agaatttttc gacaaacaaa gaagttatta caccaccatg cactctgacg
     6901 aaatatacac ctcttcacca ttagcaggtc ataaagaatt aaaaaccgat tcgaaagaac
     6961 ccatgattgt ttacattcga aaatctcaat tatctatgac tggtcaagga aaaggattaa
     7021 aagaattcaa tgaaaaagta actttaaaaa acccatatat aaaagcatac tatccccacc
     7081 gccattattg gggagctgat agtagagatt ttgtagaagc atttttgttt gattctatgt
     7141 ctgatatgga aaaatcaagc gataaagacg atgaattaat aaaagtagcc tggccaaaag
     7201 aagaagacag aaaagctttt tttgacgaaa tgagaaaatc ttttacggga cttcatggtg
     7261 atttcatcta ccacaatgag cccacaatgt ctaaataaca tccaaattaa tctaaaacaa
     7321 ctccatttca ctaattgaaa cggagttgtt tcttttagaa cacaatccta ataaattaga
     7381 ttctaagcaa atacaaaatc acgaaaaaaa ataatgaaat ttatatgaaa aagttttgca
     7441 aaagcgaaaa acctgtctat atttgcactc gcaatcacga aatgatagca acctaataaa
     7501 atagggcgat tagctcagct ggttcagagc acctcgttta caccgagggg gtcgggggtt
     7561 cgaacccctc atcgcccac
//
LOCUS       DAOSNN010000120         7571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9701_length_7571_cov_4.693321, whole genome shotgun sequence.
ACCESSION   DAOSNN010000120 DAOSNN010000000
VERSION     DAOSNN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9701_length_7571_cov_4.693321"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLQ70_07215"
     CDS             complement(<1..434)
                     /locus_tag="PLQ70_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34444.1"
                     /translation="MRQFTFLFFIGILISLSSCRTDFETIPSTGDLKFSKDTIYLDTV
                     FTNIGSSTYTLKVYNHSKNDITIPTIQLGKGLASKYRMTVDGMQGNQGKIFNNVNLLA
                     KDSLYIFIETTANIADANPIDFLYTDQIQFDGGSNLQKVELVT"
     gene            561..998
                     /locus_tag="PLQ70_07220"
     CDS             561..998
                     /locus_tag="PLQ70_07220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HQE34445.1"
                     /translation="MTFDKEKILHYCNSISENTLMQTLNIKYTDAGPDFLVATMPVNP
                     SVHQPMGLLHGGATVALAESVGSAASFLFVNGEQSEVRGIEISANHVKSKRNGIVTAT
                     ARIMHKGSSIHLWEIRITDENESLISICKLTTMILPKRKSKDL"
     gene            998..2041
                     /locus_tag="PLQ70_07225"
     CDS             998..2041
                     /locus_tag="PLQ70_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HQE34446.1"
                     /translation="MEKILEKVKTHYNQNLPFVIYNKPNTDNIIGLLQQNDNLFEVKD
                     FNEKGFVFASFDGNQTYIIPENQSEIISANFQIKENSITNIQLNSPDATVKNQFEDLV
                     TKGIQAIANNEFKKVVLSRKETLNLVDFDLIDTFKKLVQTYPTTFVYGFHHPKIGTWL
                     GATPEQLLKANEDEFHTMALAGTQKDTGSNEVVWPKKEQEEQQFVTDYIVDKLKNVAS
                     EVLVSKPYSLKAGSIWHIKTDISGTLKSSSSLQQVIQLLHPTPAVCGLPKLESKKFIL
                     ENENYARTFYTGFLGELNMNNKTDLFVNLRSMEISNSQAHLFMGCGITKDSIPEKEWE
                     ESINKSGTMKKVL"
     gene            2204..2920
                     /gene="bshB1"
                     /locus_tag="PLQ70_07230"
     CDS             2204..2920
                     /gene="bshB1"
                     /locus_tag="PLQ70_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HQE34447.1"
                     /translation="MKLDILAFGAHPDDVELGCAGTLAKEISLGKKVGIIDLTRGELG
                     TRGSVEIRNQEAADAAKILGISIRENLDMRDGFFVNDEVHQLKIIEMIRKYQPEIVIC
                     NAIDDRHIDHGKGSQLVSDSCFLSGLMKIETEHKGDKQQVWRPKLVYHYIQWKNITPD
                     FVVDITGFNEKKVEAILAYGSQFYNPNSNEPETLIASKNFLESLNYRAQDLGRLIGTD
                     FAEGFTVERYLAVNSLSNLM"
     gene            2984..3057
                     /locus_tag="PLQ70_07235"
     tRNA            2984..3057
                     /locus_tag="PLQ70_07235"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3018..3020,aa:His,seq:gtg)
     gene            3229..3558
                     /locus_tag="PLQ70_07240"
     CDS             3229..3558
                     /locus_tag="PLQ70_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HQE34448.1"
                     /translation="MGTFLISKRKNDEFQFVLKAGNGQVILASEGYTTKAACDNGIES
                     VKKNSQDDARFDKLESKNGKFYFNLKATNGQIIGNSEMYESIAARDNGIESVKKNAPD
                     ADVKEDL"
     gene            complement(3633..4661)
                     /locus_tag="PLQ70_07245"
     CDS             complement(3633..4661)
                     /locus_tag="PLQ70_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HQE34449.1"
                     /translation="MNYLKQIVILSTLQLLTISCSSQTAKVSTVEPTNYINKITSADL
                     KTYLQVIASDAMEGRETGSAGQKLAGDYLINQYKSFKIPFPKGAKDYYQKVPADFMNA
                     KKNENLPDSENIWAYIEGSEKPNEVIVVSAHYDHVGVKNGEVYNGADDDGSGTVALLA
                     IAKTFAKAKKEGHGPKRSILILHVTGEEHGLHGSRFYSENPLFPLANTITDINIDMIG
                     RRDDLHKDSNNYVYLIGSDYLSTDLYNICEEVNKKYVNLFLDYKFNDRSDPNRFYYRS
                     DHYNFAKHGIPSVFLFNGVHADYHKPTDEVDKIEFDALAKRTQLAFAIAWELANRENR
                     PVVDKSGN"
     gene            complement(4692..5465)
                     /locus_tag="PLQ70_07250"
     CDS             complement(4692..5465)
                     /locus_tag="PLQ70_07250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQE34450.1"
                     /translation="MKNINYLFLAMLTYSSVSFAQLKPVQYKDGSQILNGFAIQPSKK
                     STQKTGILILPAWMGIDKLSKDTAENLSKLGYYAFVADIYGEGNYPKDYKDAGKNAGF
                     YKTNYKEYHKRISLALKQLILSGANPDNIVVIGYCFGGTGALEVARAHLNVKGVVSFH
                     GGLGKDSTRVAEPISAKVLVCHGADDPYESKEEIATFQQEMRDTKADWQMIYYANAVH
                     SFTNPEAGNDNSKGAAYNEKAARRSWEHLKIFLDEVLKK"
     gene            complement(5733..6185)
                     /locus_tag="PLQ70_07255"
     CDS             complement(5733..6185)
                     /locus_tag="PLQ70_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQE34451.1"
                     /translation="MEILDKFDILIIKELEKDGRMAYSTIASHLKISNTMVHQRVNRL
                     IEQGILLGIKPVINEKKVGYDWGSFTGITLNKDQDSTRIIEELKKIPEITECYYISGL
                     YTLYIKICAQNHEHMRKILYEKIDNIPGISKTDSIMELGCAFKRNVSL"
     gene            6336..>7571
                     /gene="rocD"
                     /locus_tag="PLQ70_07260"
     CDS             6336..>7571
                     /gene="rocD"
                     /locus_tag="PLQ70_07260"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HQE34452.1"
                     /translation="MLLNHPTLSSKAEEMIAKENQYGAHNYHPLPVVLKRGEGVFVWD
                     LEDKKYFDFLSAYSAVNQGHCHPKIVNAIIQQAQTLTLTSRAFYNNQLGNYEEYVTHY
                     FDFDKVLPMNTGAEAVETALKLCRKWAYEVKGIPENQAQIIVCENNFHGRTTTIISFS
                     NDETARKSFGPFTSGFIKIPYDDIEALENVLQSTPNIAGFLVEPIQGEAGVYVPSDGY
                     LAKAKALCEQNNVLFIADEVQTGIARTGKLLAVHHENVQPDILILGKALSGGVYPVSA
                     VLANDAIMNVIKPGQHGSTFGGNPIAAAVAIAALEVIKVEKLAENAERLGIILRKGLN
                     EIAQRNSLITLVRGKGLLNAIVIDCDEESDLAWEICMKFSEYGLLAKPTHGNKIRLAP
                     PLVITETQIQECLDIIERAL"
BASE COUNT         2510 a         1232 c         1361 g         2468 t
ORIGIN      
        1 gttactaatt ctaccttttg aagattactt cctccatcaa attgaatttg atccgtataa
       61 agaaaatcga ttgggttggc atcggcaata ttggcagtgg tttcaataaa aatatacaaa
      121 ctatctttgg ccaaaagatt cacattatta aatattttac cttgattgcc ttgcattccg
      181 tcaactgtca ttcggtattt tgaagctagt ccttttccta attgaatggt tggaattgtg
      241 atgtcatttt tgctatgatt gtacactttt agcgtataag tactcgagcc aatgttcgta
      301 aaaacagtat ctaaataaat ggtgtctttt gagaatttta aatctcctgt gcttggtatg
      361 gtttcaaaat ctgttcggca tgaactaaga gaaataagga ttccaataaa aaaaagaaaa
      421 gtaaactgac gcatttcgat tgaatttttt gtaaaaatag caaattctat ttggaataaa
      481 gaaggttttt caaatttgaa gttataatag catttgtttt tactctcttt tatctaattt
      541 tacactttaa agcaaaatca atgacattcg acaaagaaaa aatccttcat tattgcaatt
      601 caatttcaga aaatactttg atgcaaacgc ttaatatcaa atacaccgat gctggacctg
      661 attttttagt agcaacaatg cctgtaaacc cttcagttca tcaacctatg ggattgttgc
      721 acggcggagc tacggtggct ttggcagaaa gtgttgggag tgcggcctct tttttatttg
      781 taaacggaga acaaagcgaa gttcgaggaa ttgaaatctc ggccaatcac gtcaaatcaa
      841 aacgaaatgg aatagttacg gcaactgcta gaattatgca caaaggaagc agtattcatc
      901 tttgggaaat tcgaattacc gacgaaaatg agtctttaat ttctatttgc aaattgacta
      961 ctatgatact tccaaagcga aaaagcaaag atttataatg gaaaaaattt tagaaaaagt
     1021 caagacacat tataatcaaa acttaccttt tgtaatttat aacaaaccca atacggataa
     1081 cataataggg cttttacagc aaaacgataa cttgttcgaa gttaaagatt tcaacgaaaa
     1141 aggctttgtt tttgcctctt ttgacggaaa ccaaacctac ataattcctg aaaatcagtc
     1201 tgaaattatt agtgctaatt ttcaaattaa agaaaactct atcactaata tacaattaaa
     1261 ttcacctgat gcaaccgtta aaaatcagtt tgaagatttg gttaccaaag gaattcaagc
     1321 catagcaaat aacgaattca aaaaagtagt tttatctcga aaagaaacct tgaatttggt
     1381 tgattttgat ttgattgaca cctttaaaaa attggttcaa acctatccaa ctacttttgt
     1441 atatggtttt catcatccaa aaataggaac ttggctgggt gcaacgcccg aacaattgtt
     1501 gaaggccaat gaagatgaat ttcatactat ggctttggca ggaacgcaaa aagatacagg
     1561 ttccaatgaa gttgtttggc caaaaaaaga acaagaagaa caacaattcg tcaccgatta
     1621 tattgtagat aaattgaaaa atgtggcttc ggaagtatta gtttcaaaac cttatagttt
     1681 aaaagcagga agtatttggc atataaaaac tgacatttct ggaactttaa aatcgagttc
     1741 aagtttgcaa caagtcattc aattattgca tccaacaccc gccgtttgtg gtttgccaaa
     1801 acttgagtct aaaaaattta tcttagaaaa cgaaaattac gcccgtactt tttacactgg
     1861 ctttttggga gaattaaata tgaataacaa aaccgatttg tttgtaaatt tgcgctcaat
     1921 ggaaattagc aattcacaag ctcatctatt tatgggatgc ggaattacca aagatagtat
     1981 tcctgaaaaa gagtgggagg aaagcatcaa taaatcgggt acgatgaaaa aagttttgta
     2041 aaatggaacg cagataaaac ggatttactt cgtaaagtcg cagataaaac ggattttaaa
     2101 aattatgtat gagtttagta actattaaaa tctgtttcat ccgtattttc gcatagcgaa
     2161 tcggtgttta tccgtgtact aattaatatt aataaaaaaa gaaatgaaat tagacatatt
     2221 agccttcggt gcacatccag atgatgtaga attaggttgt gcaggaacat tagccaaaga
     2281 aatttcgtta ggaaaaaaag tgggaatcat agatttgact cgaggtgaac taggaactcg
     2341 tggttcagtt gaaatcagga atcaagaagc ggctgatgct gccaaaatat tgggcatttc
     2401 tattcgggag aatctggata tgcgtgatgg tttttttgtg aatgatgaag tacatcaatt
     2461 aaaaatcatt gaaatgattc gcaaatacca gcctgaaatt gtgatttgca atgccattga
     2521 tgaccgccat atagatcacg gaaaaggaag tcaattggtt tcagactctt gttttttgtc
     2581 tggattaatg aaaattgaaa cggaacacaa gggtgataaa caacaagttt ggcgacctaa
     2641 attagtctat cattacattc aatggaaaaa cataacaccc gattttgtgg tggatataac
     2701 agggtttaat gagaaaaaag tcgaagctat tttggcctac ggttctcagt tttataaccc
     2761 caattctaat gaacccgaaa ccttgattgc atcgaaaaac ttcttggaaa gtcttaatta
     2821 tcgcgctcag gatttgggaa gattaattgg tactgatttt gccgaaggtt ttacagtcga
     2881 aagatatttg gctgtcaata gtttaagtaa tttgatgtaa tttttttatt tttttctttg
     2941 caaaagcaaa cttaagtatt atatttgcac tcgttaaaaa atggtggttg tagctcagct
     3001 ggttagagta ttggtttgtg gtgccgaggg tcgccggttc gaacccggtc agccacccag
     3061 aacgcaaaag cttcgaagaa attcgaggct ttttttgtgg attaaagttt ataaaaatgt
     3121 tacttatttt ttattctttt gaatttatta ataattttta taatttagta gctgttagat
     3181 atttgtattt ctaatttttg agtaataaat taacaacaga taaattttat gggaacattt
     3241 ttaattagta aaagaaaaaa cgatgaattt caattcgttt taaaagcagg aaacggacaa
     3301 gttattttag caagtgaagg ctatacaacc aaagcagctt gtgataacgg aatagaatcc
     3361 gttaaaaaga attcacaaga tgatgctcga tttgataaat tagaatcaaa aaacggtaaa
     3421 ttttatttta atttgaaagc tactaatgga caaatcatcg gcaatagcga aatgtatgaa
     3481 tccattgcag caagagataa cggaatcgaa tcggttaaga aaaatgcccc agatgcggat
     3541 gtgaaagaag atttgtaaaa tatatttcat taagaacaaa agcctcggat aaaattcgag
     3601 gcttttttta ttgaagtaag aatccaaaaa gattaatttc cgcttttatc caccacagga
     3661 cgattttctc tattagctaa ttcccaggct attgcaaagg caagttgcgt tcttttggct
     3721 aaagcatcaa attcaatttt atcgacttcg tctgtaggtt tgtgataatc tgcatgaaca
     3781 ccattaaaaa gaaaaaccga tggaataccg tgtttggcaa aattgtaatg atcagatcgg
     3841 tagtaaaaac gatttggatc gcttctgtca ttgaatttat aatctaaaaa caaattgaca
     3901 tattttttat taacctcttc gcaaatgtta tacaaatcgg tcgaaagata atcggaacct
     3961 attaaataca cataattatt agaatcttta tgcaagtcat cacggcgacc aatcatatca
     4021 atattaatat cggttatggt gtttgccaac ggaaataagg ggttttcaga atagaatcta
     4081 gaaccgtgaa gtccgtgttc ttcgccggtt acgtgaagaa ttaagataga acgtttgggg
     4141 ccgtgacctt cttttttagc tttggcaaag gttttagcaa ttgctaataa agctacagtt
     4201 cccgatccat catcatcggc tccattatat acttcgccat ttttcacgcc aacatggtca
     4261 taatgtgccg aaacaacaat tacttcatta ggtttttctg atccttcgat ataagcccaa
     4321 atgttttcgg aatcgggcaa attttcgttt ttctttgcat tcataaaatc cgcaggaact
     4381 ttttgataat aatctttggc accttttgga aatggaattt tgaatgattt atattgattg
     4441 ataaggtaat ctcctgctaa tttttgacct gccgaacccg tttcgcgacc ttccattgca
     4501 tccgaagcta tgacttgaag atacgttttt aaatctgctg atgtgatttt atttatataa
     4561 ttagtaggtt ctacggtact aactttagct gtttgactcg aacaacttat cgtcaagagt
     4621 tgtagggttg ataaaataac aatttgtttt aaataattca ttttgctgag tattaaatta
     4681 tagttttgct tttatttttt gagcacttca tccaagaaaa ttttcaaatg ttcccacgaa
     4741 cgtcgggcag ctttttcgtt ataagcagca cctttagaat tgtcgtttcc tgcttcggga
     4801 ttggtgaatg aatgcacagc attggcataa taaatcattt gccaatcggc tttggtatct
     4861 cgcatttctt gttgaaaagt cgcaatttcc tctttcgatt catagggatc atctgctccg
     4921 tgacaaacca aaacttttgc agagatgggt tctgcaactc ttgtgctatc tttacccaaa
     4981 ccaccatgaa aagaaacaac acctttgaca ttcaaatgag ctcgtgctac ttcaagtgcg
     5041 cctgttcctc caaaacaata gccaataact actatgttat ctggattggc accagaaaga
     5101 attaattgtt ttaatgctaa cgaaattcgt ttgtgatatt ctttgtaatt ggtcttgtaa
     5161 aaacctgcat ttttacccgc atctttataa tcttttggat aattgccttc gccataaata
     5221 tcagcaacaa aagcataata gcctagttta gataaatttt cggcagtatc tttagaaagt
     5281 ttgtcaattc ccatccaagc cggaagaatc aatattccgg ttttttgagt actttttttc
     5341 gatggttgaa tagcaaatcc gttcaaaatt tgtgaaccat ccttgtattg aacaggtttc
     5401 aattgggcaa acgaaacgct agaataagtt agcattgcca agaacaaata gtttatattt
     5461 ttcataaatg agattgtttt acacaaatat ctaaaattaa acagaatagt tggttataaa
     5521 cagttgttaa ttaagtaatt tttatcaagc tgaatattaa ataatagggc tctcctcaaa
     5581 gagtaccata tgtttttttt gcgactgatt acacagattc tcacaaattt attacacttt
     5641 ttgaaaaatc tgtataaatc tttttaatcg gtggctaatt ctatctgctt atagggtaat
     5701 aaacctgata ttattttttt ctcattgctt agttacaaac taacatttct cttaaaagca
     5761 caacccaatt ccataatcga atcggttttt gaaataccgg gaatattatc tattttttcg
     5821 taaagaatct tgcgcatatg ttcgtgattt tgtgcacaaa ttttgatata aagtgtgtac
     5881 aacccagaaa tataatagca ttctgtaatt tcgggaatct tcttcaattc ttcgataatt
     5941 cgagtagaat cttggtcttt atttaaagta attccggtaa aagagcccca atcatatccc
     6001 acttttttct cgttgattac gggttttatt cctagcaaaa tgccttgttc tattaaccga
     6061 ttcacacgtt ggtgtaccat tgtattcgag attttgagat ggcttgcaat ggtagaatag
     6121 gccattcgtc cgtctttttc caattcttta ataattaaaa tatcaaattt gtcgagtatt
     6181 tccataggtg ttaaaattaa attgacttaa attttataat aaaaatgcaa tatgacttat
     6241 tttatgtaaa tgtaagtcaa aaactagtat ttattgcgtc taaataaaaa ataaaattaa
     6301 ttttgtattt ataaatttca ttaaaacaga ttattatgtt actaaatcac ccaacacttt
     6361 cttcaaaagc agaagaaatg atagctaaag aaaatcaata cggagctcac aattaccatc
     6421 ctttgccagt agttttgaaa aggggagaag gtgtctttgt ttgggattta gaagacaaga
     6481 aatatttcga ttttctgtcg gcttattcgg cggtaaatca aggacattgc catcctaaaa
     6541 ttgtgaatgc aataattcaa caagcgcaaa ccttgacctt gacttcgcgt gctttttata
     6601 ataaccaatt gggaaattat gaagaatatg taactcatta ttttgatttt gataaagtat
     6661 tgccaatgaa tacaggagcc gaggctgtgg aaaccgctct taaattatgt cgaaaatggg
     6721 cgtatgaagt gaaaggaatt cctgaaaatc aagcccaaat tattgtttgc gaaaataatt
     6781 tccacgggag aactaccact attatttctt tttcgaatga cgaaacggca cgcaagagtt
     6841 ttggaccttt tacttctgga tttatcaaaa ttccgtatga cgatatcgaa gctttggaaa
     6901 atgttttgca atctacccca aatattgcgg gtttcttggt agaacctatt caaggtgaag
     6961 cgggtgttta cgtaccttcc gacggttatt tggcaaaagc caaagcgctg tgtgagcaaa
     7021 ataatgtttt attcattgcc gacgaagtgc aaaccggaat tgcaagaacc ggaaaattat
     7081 tggccgtgca tcacgaaaac gtacaacccg atattttgat tttaggaaaa gcactttcgg
     7141 gtggcgttta tcctgtttcg gcggttttgg ccaatgatgc cattatgaat gttattaaac
     7201 ccggacaaca cggttctact tttggcggaa accctattgc tgctgctgtt gccattgctg
     7261 cgctagaagt gatcaaagtc gaaaaactag ccgaaaatgc agaacgttta ggtattattc
     7321 tcagaaaagg attgaacgaa attgcccaac gcaattcatt aataacctta gttcgtggca
     7381 aaggattgtt aaacgccatc gtcattgatt gcgacgaaga atccgatttg gcttgggaaa
     7441 tttgtatgaa attcagcgaa tacggattat tagccaaacc cactcacggc aataaaattc
     7501 gattggcgcc accattggtt attaccgaaa cccaaattca ggaatgttta gatattattg
     7561 agagagcgtt g
//
LOCUS       DAOSNN010000121         7561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9726_length_7561_cov_5.859313, whole genome shotgun sequence.
ACCESSION   DAOSNN010000121 DAOSNN010000000
VERSION     DAOSNN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9726_length_7561_cov_5.859313"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /gene="gldK"
                     /locus_tag="PLQ70_07265"
     CDS             complement(<1..1206)
                     /gene="gldK"
                     /locus_tag="PLQ70_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldK"
                     /protein_id="HQE34453.1"
                     /translation="MKKFIAFAAIITLLIGCGKISDKGELVGTKGAKWHPEKPYGMTL
                     VPGGSFIMGKSDEDVAGVGDATTKTVTVRSFYMDETEITNREYRQFVEWVKDSTMRVR
                     LAILADESGIKPGATNAKGKGKNAGSIGDFAFNDQDPAKMTAYDKYMYDNYYSIGTDD
                     DPYAGRKLNRKVKLIKDTKLYPDEYYTEVMDSMYLPLEASYNGLRTIDVDKLKFRYSW
                     MDIQAAAKAKVGKRKDFIRTEQVKVYPDTTVWIKDFSYSYNEPMHNDYFWHQAYGDYP
                     VVGVNWKQAKAFCAWRTLNKNTYIKSKKKGHDQTNNFRLPTEAEWEYSARGGLESATY
                     PWGGPYTKNDRGCFLANFKPMRGDYAADQALYTVEAKSYEPNGYNLYNMAGNVAEWTE
                     SAYDPNAYEY"
     gene            complement(1476..2624)
                     /locus_tag="PLQ70_07270"
     CDS             complement(1476..2624)
                     /locus_tag="PLQ70_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HQE34454.1"
                     /translation="MEFDFLKPLDNEILEIIKGFSSQQLGNKVVFHTAEEFPDLNQVS
                     LAIIGVLENRGDANQTQEVDLTHIRKELYGLFPGNWNTSIADLGDIVEGNSISDTHFA
                     LRKVVSSLIKKKIIPIVIGGSQDLTYALYRAYDDLEQMVNLVSIDSKFDFGKESESIS
                     ASSYLTKIIIEEPNNLFNYSNVGYQTYNNSQEEIDLIDKLFFEGYRLGEISNNIALAE
                     PVFRDADLVSLDLTSVQSAASGNFTSFTPNGFSGKEICALSRYAGISDKVSLFGVFNH
                     NNSKQESVLIAQIIWYFIEGYQYRSNEYPFGSRENYIKYIIPLEEELVFFKSNKTDRW
                     WIEIPFISTSNNKLKKSSLLPCSYEEYLGACNQEIPERWWKAQRKNIV"
     gene            complement(2628..5147)
                     /gene="topA"
                     /locus_tag="PLQ70_07275"
     CDS             complement(2628..5147)
                     /gene="topA"
                     /locus_tag="PLQ70_07275"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQE34455.1"
                     /translation="MAKNLVIVESPAKAKTIEKFLGSDFQVESSYGHIADLPSKEIGV
                     DVENGFKPKYEVSADKKALVSKLKSLSKNAETVWLASDEDREGEAISWHLSEELKLDK
                     KKTKRIVFHEITKTAILKAIDNPREIDYNLVNAQQARRVLDRLVGYELSPVLWRKVKG
                     GLSAGRVQSVSVRLIVEREREIQNFNPVASYSVVAEFTNEAGKSFKAKLPKNFNTKKE
                     AEDFLKKNIGSTYKVSDLETKPTKKSPTAPFTTSTLQQEAARKLYLPVGITMQLAQRL
                     YEAGLITYMRTDSVNLSKEAMEAAQAEIIKSYGKEFSKPRTFANKSKGAQEAHEAIRP
                     TDMSRHTVNIDRDQARLYDLIWKRTLASQMSDAELERTNVKIEANNHGEPFSASGEVL
                     LFEGFLKVYLEGHDDDEEEQEGMLPVMKVNEKLTNNYITATERYSRPAARYTEASLVK
                     KLEELGIGRPSTYAPTISTIINRNYVEKGNLEGQERNYTQLTLQSGTVSDKVLKENTG
                     SDKGKLVPTDIGTIVTDFLVKNFGNILDYNFTAKVEQDFDEIAEGNIEWAKMMQEFYD
                     KFHPNVKDVEANADRESGERILGTDPASGKPVSVRLGKFGPMAQIGAADDEEKKFASL
                     MNDQNIGNITLEEVLDLFLLPKNLGTYKGEEVEVSNGRFGPYVRHGSVFVSLPKGENP
                     LDVTIDRAKELIDDKAKADAPIAIYKGEDVQKGTGRFGPFIKWNGIFINVSKKYNFDK
                     LSQADIEELIEDKLQKNIDKVLHNWVDEGILVEKARWGRSVITKGKIKIELSKDVDAT
                     KLTLEEVQEMIAKKTPAKKVAAKKTTAAKKPAAKKPAAKKK"
     gene            complement(5341..6041)
                     /locus_tag="PLQ70_07280"
                     /pseudo
     CDS             complement(5341..6041)
                     /locus_tag="PLQ70_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
     gene            complement(6031..7080)
                     /locus_tag="PLQ70_07285"
     CDS             complement(6031..7080)
                     /locus_tag="PLQ70_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQE34456.1"
                     /translation="MGKIKEMKFEIKLQNHFWFWGVYFLLNFLRWGAYFNDYGYSFKS
                     NLIEFALHIPLVYFNLFVLVPGFVLKKKYYQYALALLLSLMVVYLLKTGMTYYIISEN
                     IWPEANRDYKPFEINHIVAVCIGELYVLAMASSVYLTLTWLKERDRNRALREEQFKIK
                     LKYLKNQIQPHFFFNTLNNLYALSLESSDKVPDVIIKLSKLMEYVLYDIEGTKFVPLI
                     KEIDYIQNYIEIEKLRFENVQVAINIESNIDEAKVPPLLFISLLENAFKHGGYNNSKL
                     KIKINCKIVDDFLHFEIINNFVLSPSIKTKKGIGLTNTKKRLKLIFKNNFVLEHQIKF
                     NFYIIHLQIPIYNED"
     gene            7358..>7561
                     /locus_tag="PLQ70_07290"
     CDS             7358..>7561
                     /locus_tag="PLQ70_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQE34457.1"
                     /translation="MKQIILIFMIIFTAHLASAQVKTVKGTVSDASGLPLPMANVNVQ
                     GETKGTTTDMDGNFSIQVEKGKTL"
BASE COUNT         2367 a         1391 c         1111 g         2692 t
ORIGIN      
        1 atattcgtat gcattaggat cataagcaga ttctgtccat tcggccacat ttcctgccat
       61 attatacaaa ttataaccat ttggctcata tgattttgct tctacggtat ataatgcttg
      121 atcagctgca taatcacctc tcattggttt gaagtttgct aagaaacatc ctctgtcatt
      181 tttagtgtaa ggacctcccc aaggataagt tgctgattct agacctcctc tagccgaata
      241 ttcccattct gcttcggtag gcaatctaaa attattagtc tgatcgtgac ctttcttttt
      301 agatttgata taagtatttt tatttaaggt tctccaagca caaaatgctt tggcttgttt
      361 ccaatttacc cctacaacag ggtagtcacc atatgcttga tgccaaaaat aatcgttgtg
      421 cattggctca ttataagaat atgagaaatc ttttatccaa acagtggtgt caggatacac
      481 ttttacttgt tcagttctaa taaaatcttt tcttttacct actttagctt ttgctgcagc
      541 ttgaatatcc atccaagaat atctaaattt caatttatct acatcaatag ttctgagtcc
      601 gttatacgaa gcttcaagag gcaaatacat tgaatccata acctcagtat aatattcgtc
      661 tggatataat ttagtatctt ttatcagttt tactttgcga ttgagttttc ttccagcata
      721 aggatcatca tctgttccaa tactataata gttatcatac atatatttat catatgcagt
      781 cattttagct ggatcttgat cattgaaagc aaaatcacca atgctacctg cattttttcc
      841 tttacctttt gcgtttgtag cacctggttt tattccagat tcatcagcaa gaatagctaa
      901 acgaactctc attgtagaat cttttaccca ttctacaaac tgacggtatt ctctgttggt
      961 tatctcggtt tcatccatat agaacgaacg aacggtgact gtttttgtag ttgcatcccc
     1021 aacaccggcg acatcctcat cagattttcc cataataaaa gaacctccag gaactaatgt
     1081 cattccataa ggtttttcag gatgccattt tgcaccttta gtaccaacta actctccttt
     1141 gtcggatatt ttgccacatc caattagcaa ggttatgatt gcagcaaaag caataaactt
     1201 cttcatatta ataggggttg attattcatt attattaagc ccgtaaacct atttattatt
     1261 atttaataaa acaattattt agagtactat ttattacata gcccaaaact aaaattaatt
     1321 ttaataagaa aaaaatattt tatcgataaa attcaaaaat agacgataaa aaacacattt
     1381 tttaaaatat tataagtttt gtcttataaa ataaattaga tgagttttga attaagaatt
     1441 ctttagtatt atttcttact acaacaaaca aaagtttaca ctatattttt acgctgtgct
     1501 ttccaccatc tttcaggaat ttcttgatta caagcgccca aatactcttc ataagagcaa
     1561 ggtaataacg agcttttctt taacttatta ttgcttgttg aaataaatgg tatttctatc
     1621 caccatctat cggttttgtt gcttttaaaa aaaaccaact cttcctcaag tggaatgata
     1681 tattttatat aattctcacg gcttccaaaa ggatattcat tagaacgata ctgataacct
     1741 tctataaaat accaaataat ttgagcaatc aaaacggatt cttgtttaga attattgtga
     1801 ttaaagaccc caaaaagcga aactttatca ctaattcctg catatcttga gagagcacaa
     1861 atctctttgc cactaaatcc attaggagta aaggatgtaa aattgcccga agctgccgat
     1921 tgtacagatg tcaaatcaag acttaccaaa tcggcatctc taaaaaccgg ttctgctaat
     1981 gcaatattat tcgatatttc acctaaacga tacccttcaa agaataattt atctattaaa
     2041 tctatttctt cttgtgaatt attataagtt tgatacccaa cattactata attaaaaaga
     2101 ttgttgggtt cttcgataat tatttttgtt aaataagagg atgccgagat agactcgctt
     2161 tcttttccaa aatcgaattt actatcaatt gaaaccaaat tgaccatttg ttctaaatcg
     2221 tcataagctc ggtaaagtgc ataggtcaaa tcctgtgaac caccaataac aataggaatg
     2281 attttctttt tgattaaact agaaactact tttctcagcg caaaatgtgt atctgaaata
     2341 gaatttcctt caacaatatc gcccaaatca gcaatagaag tattccaatt tcctggaaac
     2401 aaaccataca attcttttcg aatgtgtgtt aaatctactt cctgagtttg attggcatca
     2461 cctctatttt ctaaaacccc aatgattgca agactaactt gattcaaatc agggaattct
     2521 tcagcagtat gaaatacaac cttattcccc aattgctgag aagaaaatcc cttgataatt
     2581 tcaagaattt cattgtctag aggtttgaga aaatcaaatt ccatatatta tttctttttt
     2641 gctgctggtt ttttggcggc tggtttttta gccgctgtag ttttcttagc ggctactttt
     2701 ttggctggtg ttttcttggc aatcatttct tgaacttcct ccaatgtcaa ttttgtagca
     2761 tcgacatctt tgctcaattc aattttgatt tttcctttag taataaccga tcttccccag
     2821 cgtgcttttt cgaccaatat tccttcatcg acccaattat gcaaaacctt gtcaatgttt
     2881 ttttgtaatt tatcttcgat caattcttca atatcagcct gagacaattt atcgaaattg
     2941 tattttttgc ttacattgat aaaaatgcca ttccatttga taaatgggcc aaaacgacct
     3001 gttccttttt gaacatcctc acctttataa atagcaattg gcgcatcggc ttttgcctta
     3061 tcatcaatta attcttttgc tctatcaatg gttacatcta atggattttc gcctttaggc
     3121 aaagaaacaa aaacgcttcc gtgacgtaca taaggaccaa aacgaccgtt acttacctca
     3181 acttcttctc ctttatatgt gcctaaattt ttaggtaata aaaataaatc taaaacttct
     3241 tctaaagtga tgtttccaat gttttggtca ttcatcaaac tcgcgaattt tttctcctca
     3301 tcatctgcag ccccaatttg tgccataggt ccaaattttc ctaaacgaac agatactggt
     3361 tttccagatg ctggatctgt acctaaaatt ctttctccac tttctctatc agcattagcc
     3421 tcaacatctt ttacatttgg atgaaattta tcgtagaatt cctgcatcat tttggcccat
     3481 tcgatatttc cttcggcaat ttcgtcaaaa tcttgttcga ccttggcagt gaaattataa
     3541 tctaaaatat ttccgaaatt cttaaccagg aaatcggtaa caatggttcc aatatcggta
     3601 ggaaccaatt tccctttatc agaacccgta ttttctttta aaactttatc cgaaactgta
     3661 cccgattgca aagtcagttg ggtataattg cgctcttgac cttcaagatt tcctttttcg
     3721 acataatttc tattgataat agtcgaaatg gttggcgcat aagtcgatgg acgaccaatt
     3781 cctaattcct ctaatttttt taccaaagaa gcttcagtat atctagcggc aggacgcgaa
     3841 tatctttcgg tagcggtaat gtaattattt gtcaatttct cattaacttt cattaccgga
     3901 agcattcctt cttgctcttc ttcatcatca tcgtgacctt caagatacac tttcaagaaa
     3961 ccttcaaaaa gcaagacttc acctgaagct gaaaatggtt cgccatgatt attggcttca
     4021 atttttacat tggtacgttc caattcagca tcactcattt gtgaagccaa tgttcttttc
     4081 caaatcaaat catacaaacg cgcttgatct ctatcaatgt ttacggtgtg tcgcgacata
     4141 tccgttggac gaattgcttc gtgagcttcc tgtgcacctt tgcttttatt ggcaaaagtt
     4201 cttggtttcg agaattcttt tccataggat ttgatgattt cggcttgagc ggcttccatc
     4261 gcctcttttg acaagtttac actgtcagtt ctcatataag ttataagccc agcttcgtat
     4321 aaacgttggg ctaattgcat agtgattcca actggtaaat acaatttcct agcagcttct
     4381 tgttgcaaag tcgatgtggt aaatggtgct gttggagatt tcttggttgg ttttgtctca
     4441 aggtctgaaa ctttataagt agaaccgata ttctttttta agaaatcttc ggcttctttt
     4501 ttagtattaa aattctttgg cagtttagct ttaaatgact ttccagcttc atttgtaaat
     4561 tctgccacaa ctgaatagga agcaacggga ttgaaattct gaatttctct ttctcgctca
     4621 acaatcaacc gaactgatac tgattgcaca cgtcctgccg aaagtcctcc tttcactttt
     4681 cgccaaagaa ctggcgataa ttcgtaaccc actaatcggt ctaaaacacg gcgtgcttgt
     4741 tgcgcattca ctaaattata atctatttct cgtggattat caattgcttt tagaatagcc
     4801 gttttagtaa tttcgtgaaa aacaattctt ttggttttct ttttatccag ttttaattct
     4861 tcggacaaat gccaagaaat agcctcccct tctcgatcct catcacttgc taaccaaacc
     4921 gtttcggcat ttttggataa agattttagc ttagaaacca atgctttttt atcggccgaa
     4981 acttcgtatt tgggtttgaa accattttct acatctactc caatttcttt ggacggcaag
     5041 tcggcaatat gtccgtaact tgactcgact tggaagtcgc ttcctagaaa tttttcgatt
     5101 gtttttgcct ttgcagggga ctctacgata actaaattct ttgccatagg tatatttttc
     5161 tgggacaaaa gtagaagaaa tttttaaata ttgtgctttt gcaaatttta aaaataaata
     5221 taatcgacag attacacaat aggaacattc aaaattttat tttttttgaa agataattca
     5281 atcgagattg caatgcattt tgctcttttg aaagttggga ttataataag gactagagga
     5341 ttattctaaa attctactct tgacgtcttt actaaaattt cgaccaatgg gaattgtata
     5401 agtaagtatt tgaattctat ttccttcgat aaatttcact ttatcaatgg caatggtata
     5461 agatttatga attcggataa agcgatctaa aggcaattct tcggataatg aagtcaagga
     5521 tttatgagta atataagttt tgtcggcggt caccaccttt atatattctt tcattccttc
     5581 gataaagaga atttcatcga tatttatttt taccattttt ttatctactt tgataaaaat
     5641 atgcgaatct accttaacga gttcggtttt taggttgttt tttaaactaa attcggttgt
     5701 gattttattg atacatttta taaatcgggg caacggaatg ggttttacca gataatctaa
     5761 aacctctaag tcgtaactct ctgcggcaaa ttctctgaaa gctgtggtga taattacttt
     5821 aggttttttt ccattaaact aatcagttca aaacccgtca tcataggcat attgatgtct
     5881 aaaaaaactg tgtcaactgc attgttgttg atgaaatcca atgcttctaa tgagttatta
     5941 aacgtgccaa caacttcaaa ttctgaaaaa ttagtaaaat aattttgcag aacttttatt
     6001 gccaaaggtt catcatcgac caaaacacat ttaatcttca ttatatatag gtatttgcaa
     6061 atggataatg taaaagttga attttatctg atgctctaaa acaaaattgt ttttgaaaat
     6121 caatttcagt cgctttttag tattggtcaa gccaattcct tttttagttt taatacttgg
     6181 tgaaagtaca aaattattta tgatttcaaa atgcaagaag tcatcaacaa ttttacaatt
     6241 tatctttatt ttgagtttac tgttgttgta tccaccgtgt ttgaaggcat tttctaatag
     6301 tgaaataaac aataaaggag gaacttttgc ttcatcaata tttgattcaa tatttatggc
     6361 gacttgcacg ttttcgaaac ggagtttttc aatttcgata taattttgaa tatagtcaat
     6421 ttctttgatt aatggaacaa atttggtacc ttcgatatca tacaaaacat attccatcaa
     6481 tttagacagt tttataatta catcaggcac tttatctgaa gattctaaag ataaggcata
     6541 caaattattt aatgtattga agaaaaaatg gggttgaatt tgatttttca ggtattttaa
     6601 tttgattttg aattgttctt ctctcaaagc acgatttcta tctctttctt tcaaccaagt
     6661 tagcgttaaa tacaccgaag atgccatcgc caatacatat aattcgccaa tgcaaaccgc
     6721 cacaatatga ttgatttcaa aaggtttgta atctctatta gcttctggcc aaatgttttc
     6781 tgaaataata taataggtca ttccagtttt aagtaaataa actaccatca aactcaacag
     6841 caaggctaag gcatattgat agtatttttt ctttaataca aatcccggaa ccagaacaaa
     6901 caaattgaaa taaaccaagg gtatgtgaag ggcaaattcg atcagattgg atttaaacga
     6961 atagccataa tcattaaaat aagcccccca acgcagaaag ttcaataaaa aataaacacc
     7021 ccaaaaccaa aaatgatttt gaagttttat ttcgaatttc atctccttta tttttcccac
     7081 tttacaattt aataattaag atttctcgct gttgattgtc taaaaataaa cttttgcaac
     7141 tttaatcaat ttgacaataa aaaacagcat tttggaaata aaattatgaa aataaataaa
     7201 aacgatttag aataaacaca agaaaacgtt ttcgaaatac ataatattga ttgaatcatg
     7261 tcgttggttg aaattttatt accgttgaga gaatatattt tttttagaat aatcacccca
     7321 ataaatttac accataacta acaaaaaata tataaaaatg aaacaaatta ttttaatctt
     7381 catgattatt tttactgcgc atcttgcctc tgcacaagta aaaaccgtca aaggtactgt
     7441 gagcgatgca agtggtttac cattgccaat ggccaacgtt aatgtgcaag gcgaaacaaa
     7501 aggaactacc accgatatgg atggtaattt ctctattcaa gtagaaaaag gaaaaacttt
     7561 g
//
LOCUS       DAOSNN010000122         7495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_9852_length_7495_cov_5.943414, whole genome shotgun sequence.
ACCESSION   DAOSNN010000122 DAOSNN010000000
VERSION     DAOSNN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9852_length_7495_cov_5.943414"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLQ70_07295"
     CDS             complement(<1..1064)
                     /locus_tag="PLQ70_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HQE34458.1"
                     /translation="MFSANDFIPTAYSHSIEKGNFEWSSPSNIALVKYWGKKDSQIPA
                     NPSVSFTLNNCKTITKLAFDKKQNQDTFSFDLLFEGKPKEDFKPKIQQFFERVLVYLP
                     FLKDYHFTIDTQNTFPHSSGIASSASGMAALAMNLMSLEKVLNPEMSDDYFYHKASFL
                     ARLGSGSACRSVKGEVVVWGNHNNIQGSSDLFGVAFPKAIHENFKNFQDTILLVDKGE
                     KQVSSSVGHNLMNGHPFAERRFKQAHENLDKLISVFESGNLEEFITIVESEALTLHSM
                     MMTSIPYFILMKPNTLQIINAIWKYRNETKIPVCFTLDAGANVHVLYPETVKETVLQF
                     IKDELVGYCQNGQYLCDQIG"
     gene            1158..1634
                     /locus_tag="PLQ70_07300"
     CDS             1158..1634
                     /locus_tag="PLQ70_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HQE34459.1"
                     /translation="MNKISKILVVVATCLVVGYFSGVVTRSAITTWYPTLVKPSFNPP
                     NWIFAPVWSMLYIMMGVAAGLIWDRIDFEKEIVKKALVFFAVQLALNALWSYLFFGLK
                     NPMLAGLEIIILWLMIYETYFKFSKINKIAGYLMIPYLLWVSFASVLNASIWWLNQ"
     gene            1640..2362
                     /locus_tag="PLQ70_07305"
     CDS             1640..2362
                     /locus_tag="PLQ70_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HQE34460.1"
                     /translation="MKNLYSQILQSKLENEKLLAILLDPDKINLKNVETLIEKINQSP
                     ATHIFIGGSLVESNILDKLIIKVKQNCNLPVVLFPGNPSQISGQADGILFLSLISGRN
                     PDFLIEHQVQAVPILKKTQLEIISTGYILIESGTKTAVERVSKTKPLDRNNIEYVVQT
                     AQAGELLGNKLIYLEAGSGAKQAVPLEMIQKVAQNIKIPLLVGGGIVDLLGIQKAYDS
                     GADLVVIGTAFENNIDFFNQNT"
     gene            2363..2989
                     /gene="pnuC"
                     /locus_tag="PLQ70_07310"
     CDS             2363..2989
                     /gene="pnuC"
                     /locus_tag="PLQ70_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HQE34461.1"
                     /translation="MIEYFFSQYATYSTLDITLEIVAVIFGLLSVWYAKKDNILVFPT
                     GLVSTSIYVYLLWKWVLWGDMMINGYYFIMSIYGWYHWTRKKGDVVEFPISVISRAEK
                     RMSLIIFIFTVTFVVLVYHYFDKFTTWYAYVDTFLTGIFFVGMWLMAKRKIENWLYWI
                     IGDIISIPLYFSKGFTFTSFQYLIFTIVAIYGYLEWKKNLNNSQIKEI"
     gene            2947..5067
                     /locus_tag="PLQ70_07315"
     CDS             2947..5067
                     /locus_tag="PLQ70_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HQE34462.1"
                     /translation="MEKKLKQLSNKGDLAILKIALFGPESTGKTTLAKELAEHFQTAF
                     VPEFARDYLQEKWDTSGQICDVNDMLPIAYGQLNLENQSLVSANKFLFCDTNLMVTKV
                     FSEVYYNFCDPLLDKAARKHEYDLFFLTDIDVPWEKDDLRDKPEGRETIFSVFKQSLI
                     DNNKPFITISGTKKARLEKAIQIVDDLAKAKKMGLSSHDFVQIFEHGIAIDNIISQLD
                     LFKNGISKAILAEPAMVSKGILKLSEKDFQAKADFFDANKANFKLEKFVPASGAASRM
                     FKFLNEFLNEFDIEKETINAYINRKKASELSIFIVGMNKFPFFETVYLKLKSKFPNFD
                     SLERDYKNYYFIKYLLSPDYFDFANKPKGVLLFHRYTAHNATAIEEHFYECANYSSSN
                     GKASLHFTVSESHKMLFEDIEKNTKKKVEKESGIAIETHYSFQNKSTDTIAVNPNNKP
                     FRDENGKLLFRPGGHGALIENLNSLGADIVFIKNIDNVAQNRIKSIAMYKKALAGILM
                     ELQQQVFRYLNEMDHFQEDDLNEIVAFATNELNIELEENFRKYKLEYKIDYLKTILDR
                     PIRVCGMVKNEGEAGGGPFWVRDNRGNHSLQIVETSQVDLVNENQVKILSSSTHFNPV
                     DLVCGIRNYKNEKFDLTQFIDHNSGFIVEKNSYGKIVKSYELPGLWNGAMAKWITVFV
                     EVPLITFNPVKTVNDLLKTAHQPQ"
     gene            5070..5471
                     /gene="arfB"
                     /locus_tag="PLQ70_07320"
     CDS             5070..5471
                     /gene="arfB"
                     /locus_tag="PLQ70_07320"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HQE34463.1"
                     /translation="MEPEKIISELQFKAVRSSGAGGQNVNKVSSKVVLSFDLKNSQAL
                     SEDEKALLEFKLASRLTSDTILILNCDEDRSQLKNKAIVTKRFLDIITAGLVVPKIRK
                     ATKIPKSVIRKRIKDKNYISEIKKSRRKPDF"
     regulatory      5522..5616
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5708..>7495
                     /locus_tag="PLQ70_07325"
     CDS             5708..>7495
                     /locus_tag="PLQ70_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE34464.1"
                     /translation="MNHLFYLKRLKSKVQSPKSKLFITSSFFVLTSIFSTAQEKPKDT
                     TKVNQLDEVLVSAVRVTTKTPVSFSNLDKKEIKTRNLGQDIPILMNYLPSVVTTSDAG
                     NGVGYTGIRVRGSDATRVNVTINGIPYNDSESHGTYWVNMPDFASSLESIQLQRGVGT
                     STNGAGAFGASLNMLTETYSKKATAEISSSLGSFNTNKNTVKFSTGLLNNHFEMAGRL
                     SVLKSDGYIDRASSNLKSYFLQGTYIGNTTLIKALVFGGTEKTYQSWNGIDAATMATD
                     RTFNSAGIYTDEAGKTRFYDNETDNYKQDHYQLHWNEKLSENWSTNLAFHYTKGKGYY
                     ENYKEDAKFSSYGLTPIVIGGTTINTTDLVRQKWLDNDFYGTTFSANYKDENLNVILG
                     GGWNKYEGEHYGKVIWARYASQSELGDRYYEDQATKTDGNIFAKANYQITPKISLFGD
                     LQLRNVTYKANSWETGTVDDTFTFFNPKAGLNYDIDAKNKLYFSYARANREPNRTDYE
                     NGNPKPEKLNDYELGWRWNAEKVKINTNIYYMAYKDQLILTGKLDDVGSPIRSNSDTS
                     YRLGLEVDATIQLSKKFIIRPNFTLSDNKN"
BASE COUNT         2539 a         1196 c         1386 g         2374 t
ORIGIN      
        1 attccaattt ggtcacaaag gtactgacca ttttgacaat agccaactaa ttcgtcctta
       61 ataaattgta aaacagtttc tttcacagtt tctgggtata aaacgtgaac attggctccg
      121 gcatcgagcg tgaaacaaac cggtattttt gtttcgtttc tgtatttcca aatagcattg
      181 atgatttgca aggtgtttgg tttcattaaa ataaaataag gaatagaggt catcatcatc
      241 gaatgcaaag tcagtgcttc actttcgaca atagttatga attcttccaa attgccactt
      301 tcgaaaacag aaatcaattt atccagattt tcgtgcgctt gtttaaatcg cctttcggca
      361 aatggatgtc cgttcattaa attatgaccc acagaactcg aaacttgttt ttcgcctttg
      421 tccaccaata aaatagtatc ctgaaaattc ttgaaattct cgtgaatggc tttcggaaat
      481 gctactccaa acaaatcaga acttccttga atattgttgt gattgcccca aacgacaact
      541 tcgcctttta cgcttcggca ggcacttcca gaacccaaac gagccaagaa tgaagccttg
      601 tggtaaaaat aatcatcact catttcagga tttaacactt tttccaaact catcaaattc
      661 attgccaacg ctgccattcc tgacgctgaa gaagcaattc ctgaactgtg cggaaaagta
      721 ttttgagtat caatggtaaa atgatagtct ttcaaaaaag gcaagtaaac caaaactctt
      781 tcgaaaaatt gctgaatttt gggtttgaaa tcttctttgg gtttgccttc aaaaagcaag
      841 tcgaaagaaa atgtatcttg attttgtttt ttgtcgaaag ccagttttgt aattgttttg
      901 caattattta acgtaaaact caccgaagga ttggctggaa tttgactgtc ttttttgccc
      961 caatatttta ccaaggcgat gttactagga gaactccact cgaagtttcc tttttctatt
     1021 gaatgagaat aggcagttgg tatgaaatcg tttgctgaaa acatcaatta ttttttttgc
     1081 aaagatactt tttatcgttt aaaagtttaa ccgtttaatc gtttaaaagt tttgattaat
     1141 tttgaaaaaa aataaagatg aataaaatct caaaaattct cgttgtggtt gccacttgtt
     1201 tagttgttgg atatttttcg ggagtggtta cgcgttcggc aatcacgact tggtatccta
     1261 cattagtaaa acctagcttt aatccgccga attggatttt tgctcccgtt tggagtatgc
     1321 tttacataat gatgggagtt gcggcaggat tgatttggga tcgaatagat tttgaaaaag
     1381 aaattgtcaa aaaagctttg gtgttttttg ccgttcaatt ggcattgaat gctttgtggt
     1441 cctatttgtt ttttggttta aaaaatccaa tgttggctgg actcgaaatc ataattcttt
     1501 ggttgatgat ttatgaaacc tattttaaat tctcaaaaat caataaaatt gcgggttatt
     1561 taatgattcc ttacttgctt tgggtgagtt ttgccagtgt tttgaatgct agtatttggt
     1621 ggttgaatca ataatattta tgaaaaacct ctacagccaa attctacaat caaaattaga
     1681 aaacgagaaa ttgctcgcca ttcttctaga tccagacaaa atcaatttga agaatgtaga
     1741 aacattaatc gaaaaaataa atcagtcacc agcgacacat atttttattg gcggaagtct
     1801 tgtcgaaagt aatattttgg acaaactcat cataaaagtg aaacaaaatt gtaatttgcc
     1861 tgttgtgtta tttcctggaa atccatctca aatctcaggt caagccgatg ggattttatt
     1921 cctgtcttta atttctggta gaaatccgga ttttctaatc gaacatcagg ttcaagccgt
     1981 tccgatttta aagaaaaccc aactcgaaat tatttctact gggtatattc tcatcgaaag
     2041 cgggacaaaa acggctgtag aacgagttag taaaactaaa ccattagacc gaaacaatat
     2101 tgaatatgtt gttcaaaccg cacaagctgg cgaattatta ggcaataaat taatttatct
     2161 cgaagctgga agtggtgcca aacaagctgt tccgttagaa atgattcaaa aagtagctca
     2221 aaatataaaa atcccgttgc tagttggcgg tggaattgta gatttgcttg gaattcaaaa
     2281 agcctatgat tcgggtgccg atttggtggt gattggaaca gcttttgaaa ataatataga
     2341 tttttttaat caaaacacct agatgataga gtactttttt tcgcaatatg caacctattc
     2401 caccttggat attactttag aaatcgtcgc ggtgattttt ggtttactca gtgtttggta
     2461 tgccaaaaaa gacaatattt tggtattccc aacaggatta gtcagtacat caatctatgt
     2521 ttatttgctg tggaaatggg ttttgtgggg cgatatgatg attaatgggt attatttcat
     2581 aatgagtatt tacggttggt atcattggac aagaaaaaaa ggagatgttg ttgaattccc
     2641 aatttcggta atttcgaggg ctgagaaacg aatgagccta attattttta tatttacggt
     2701 cacatttgta gttttggtgt atcactattt cgacaaattt accacttggt atgcttatgt
     2761 agataccttc ctaacgggaa tttttttcgt aggaatgtgg ttgatggcta aaagaaaaat
     2821 cgaaaactgg ttatattgga ttattggtga catcatttcc attcctttgt atttttcaaa
     2881 agggtttact tttacgagtt ttcagtattt aatattcaca attgttgcca tttatggtta
     2941 tttagaatgg aaaaaaaact taaacaactc tcaaataaag gagatttagc aatacttaaa
     3001 attgccctat ttggccccga atctacgggg aagactactt tggcaaaaga acttgccgag
     3061 cattttcaga cagcatttgt tcctgaattt gcccgtgatt atctacaaga gaaatgggat
     3121 acttctggac aaatttgtga tgtaaacgat atgttgccta ttgcttatgg tcaattgaat
     3181 ttagaaaacc aaagtcttgt ttcggctaat aaattccttt tttgtgatac caatttgatg
     3241 gtaaccaaag tgttttcgga agtatattac aacttttgtg acccattatt agacaaagca
     3301 gctcgaaaac atgagtacga tttgtttttt cttaccgata ttgatgtgcc ttgggaaaaa
     3361 gacgatttga gggataaacc cgaaggtcgt gaaactattt tttcggtttt taaacaatcg
     3421 ttaatagata acaataaacc ttttattact atttctggta ctaaaaaggc tcgtttagaa
     3481 aaagccattc agattgttga tgatttggca aaagctaaaa aaatgggact ttcttcccat
     3541 gattttgttc aaattttcga acacggaatt gcaatagaca acatcataag tcaattggat
     3601 ttattcaaaa acggaatttc aaaagcaatt ttagccgagc ctgcaatggt ttctaaaggg
     3661 attttgaaac tttccgaaaa agattttcaa gcaaaagccg atttttttga tgccaataag
     3721 gcgaatttta aattagaaaa atttgttcct gcatcaggtg ccgctagcag aatgtttaaa
     3781 tttttgaatg aatttttgaa tgaatttgac atcgaaaaag aaaccatcaa tgcctatatc
     3841 aatagaaaaa aggctagcga attatctatt tttattgttg ggatgaataa atttccattc
     3901 tttgaaacag tctatttaaa attaaaatct aagttcccca attttgacag tttagaaaga
     3961 gattataaaa attattattt tataaaatac ctactctcac ccgattattt tgattttgct
     4021 aataaaccaa aaggcgttct tctatttcat agatacacag ctcataatgc aacagcaata
     4081 gaggaacatt tctatgaatg tgctaattat tcgagttcga atggaaaagc aagtttgcat
     4141 tttacagttt ctgaatcgca taaaatgcta tttgaagaca tcgaaaaaaa caccaaaaag
     4201 aaagttgaaa aggaatcggg aatagcaata gaaacgcatt attcttttca aaataaaagt
     4261 acagatacga tagctgttaa tccaaacaac aaaccattta gagacgaaaa tgggaaattg
     4321 cttttcagac ctggaggcca cggagcttta attgaaaatt taaatagtct tggagctgat
     4381 attgttttta ttaaaaacat agacaatgtt gctcaaaatc gcatcaagag tatcgcgatg
     4441 tacaaaaaag cattggcggg aattttgatg gaattgcaac aacaagtttt taggtatttg
     4501 aatgaaatgg atcactttca agaagatgat ttaaatgaaa ttgttgcttt tgccactaat
     4561 gaattgaata ttgaattaga agaaaatttt agaaaataca agttagaata taaaattgat
     4621 tacctaaaaa ccattctgga tcgacccatt cgtgtttgcg gaatggtaaa aaacgaaggt
     4681 gaagctggtg gaggtccttt ttgggttagg gataacagag gaaatcattc cttacaaatt
     4741 gttgaaacct cacaagtgga tttggttaat gaaaatcaag taaaaatact gtcgagttcc
     4801 acgcatttta atcctgtaga tttggtttgt ggaatccgca attataaaaa tgagaaattt
     4861 gatctgactc aatttataga tcataatagc ggttttattg tcgaaaaaaa tagttacggt
     4921 aagatagtaa aaagctatga attgcctggt ttgtggaatg gtgcgatggc aaaatggatc
     4981 actgtttttg ttgaagtgcc tttgattacc ttcaatccag tgaaaacagt caatgattta
     5041 ttaaaaacag cacatcaacc acaatagttt tggaaccaga gaaaatcata tccgaattac
     5101 agttcaaagc cgttcgcagt agtggagccg gaggtcaaaa tgtgaataaa gtgtcctcaa
     5161 aagtggtctt gtcattcgat ttgaaaaatt cgcaagcttt gtcagaagat gaaaaagcac
     5221 tgttagaatt caaattggca tcaagattaa cttctgatac tattttgatt ttgaattgtg
     5281 acgaagatcg aagtcagctc aaaaacaaag ccattgttac caaaaggttt ctggatatta
     5341 ttaccgctgg cctcgtagtt ccaaaaatta gaaaagccac aaaaatccct aaatcggtga
     5401 ttagaaaacg aataaaggac aaaaattata tttctgaaat taaaaagtcg cgccggaaac
     5461 ctgattttta atttttttgc caactaataa ttcataattc ataatttata tttaagtttg
     5521 cgtcgtctca aaggggtgct ctaaataacg agctgagatt atacccatag aacctgaaca
     5581 ggtaatgctg ttaagggaaa acagaaataa agatgaagtg cacgtcatcc gaaaagtctg
     5641 gaacaacaat catttattaa tttaacaaaa gaataattcc cccttttatt cgtaaaacta
     5701 tttacggatg aaccatttat tttatttaaa aagactaaag tccaaagtcc aaagtccaaa
     5761 gtccaaactt tttataacat ctagtttctt tgttctaact tctattttct ctactgcaca
     5821 agaaaaacca aaagacacta ctaaagtcaa tcaactcgac gaagttttgg tctctgcagt
     5881 tcgtgtaact acaaaaacgc ctgtttcttt tagtaatcta gacaaaaagg aaattaaaac
     5941 ccgaaattta gggcaagata ttcctatttt gatgaactat ttgccctcag tagtgaccac
     6001 ctccgatgct ggaaatggtg tgggttatac cggaattcga gttcgtggta gcgatgccac
     6061 tcgggtaaac gtgaccatca acggaatacc ttataacgat tctgaaagcc acggaaccta
     6121 ttgggtcaat atgcccgatt ttgcttcttc attagaaagt attcaattgc aacgcggagt
     6181 tggaacttct actaatggtg ctggagcttt tggagccagt ttgaatatgc tgactgaaac
     6241 gtattctaaa aaagcaacag ccgaaatttc aagttcatta gggagtttta atactaataa
     6301 aaacacagta aaattcagta caggtttatt gaacaaccat tttgaaatgg caggacgttt
     6361 gtctgtatta aaatctgatg gatatattga tagagccagt tctaatttga aatcctattt
     6421 tctgcaagga acctatatcg gaaatacaac cttaatcaaa gctttggttt ttggaggaac
     6481 tgaaaaaaca taccaatctt ggaacggaat tgatgctgca acaatggcaa ctgatagaac
     6541 tttcaactcg gcaggaatat atactgacga agcaggaaaa actcgttttt atgacaatga
     6601 aaccgataat tataagcaag accattatca attgcattgg aacgaaaagc tatccgaaaa
     6661 ttggagtacc aatctagcgt ttcattatac caaagggaaa ggatattatg aaaactataa
     6721 agaagatgct aaattttcga gttatggatt aactccaatt gttattggcg ggacaaccat
     6781 aaatacaacc gatttggtac gtcagaaatg gttggataac gatttttatg gcactacttt
     6841 ttcagctaat tataaggatg aaaacctaaa tgtaattctt ggtggtggct ggaataaata
     6901 cgaaggcgaa cattatggaa aagtaatttg ggctagatat gcttcgcaaa gcgaattggg
     6961 cgaccgttat tatgaggatc aagcaaccaa aaccgacgga aatatttttg ctaaagccaa
     7021 ttatcaaatc accccaaaaa taagtttgtt tggtgattta caactgcgaa atgtaactta
     7081 caaagccaac agttgggaaa caggaacagt cgatgatacc tttactttct tcaatccaaa
     7141 ggcaggtttg aattatgata ttgatgcaaa gaacaaattg tatttctcct acgcaagagc
     7201 caatcgcgag ccgaatcgaa ccgattatga aaatggaaac cccaaacctg aaaagctaaa
     7261 tgactatgaa ttgggctgga gatggaatgc tgagaaagta aaaatcaata caaatattta
     7321 ttatatggcc tacaaagacc aactgatttt gactggaaaa ctagacgatg taggaagtcc
     7381 aattcgttct aacagcgata caagttaccg tttggggtta gaagttgatg caacaattca
     7441 attgtctaaa aagtttatta ttcgacctaa ttttactttg agtgataata aaaat
//
LOCUS       DAOSNN010000123         7412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10022_length_7412_cov_4.116488, whole genome shotgun sequence.
ACCESSION   DAOSNN010000123 DAOSNN010000000
VERSION     DAOSNN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10022_length_7412_cov_4.116488"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..521
                     /locus_tag="PLQ70_07330"
     CDS             90..521
                     /locus_tag="PLQ70_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQE34465.1"
                     /translation="MNNVKSLNKWANAHTYLPVDLLRMALGIFLFWKGVTFITNIQYL
                     VDLLSPIDKLDDGMSGMLIVHYVASAHFLGGILIFFGLLTRWALIVQLPILIGAVVIN
                     FMGEMHSQNLFLSLITLVICVAFIIYGSGKHSADYYFKMQE"
     gene            complement(586..1638)
                     /locus_tag="PLQ70_07335"
     CDS             complement(586..1638)
                     /locus_tag="PLQ70_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQE34466.1"
                     /translation="MHKITYKNIHNRFKLNGIHISRKEMFYVAYSFIKEGKPFEQHIG
                     NFLLDWFDEKSYIELQTSGTTGVPKIIRIEKQAMLDSALATGDFFGLQPGDTMLHCLP
                     TNYVAGKMMWVRSFILGLDMKFVEPTSNPLEKMDECFDFCAMVPLQAKNSLEKLKQKK
                     IKKLIIGGVRVHKALEQELVKLPMEIYETYGMTETITHIAAKKIGAVAFTTLPNVSVS
                     EDKNQCLVIKAKNISKDPIVTNDIVKLISDTQFIWEGRIDNVINSGGIKLLPEQIEEK
                     LSTLIPRRYFVFGKADEVLGEKAILYVEGEPLEIEESVFGLLNKYEIPKEIIFIPKFQ
                     ETATGKIMRRESILSK"
     gene            complement(1648..2571)
                     /locus_tag="PLQ70_07340"
     CDS             complement(1648..2571)
                     /locus_tag="PLQ70_07340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQE34467.1"
                     /translation="MFIEQAYKGNNTWWRVLITTLLTAGVFIANFVTFFTMSKEQMDA
                     VYKTMSEVPNNLSLLINLSPFILLLLLLFLLVRVLHERSVLSLTTSRKSIDYKRILFS
                     FLLIVSLTIVMFIISYLVDHSNIILNFNPLKFTILFAISLLLFPCQIGLEEYLFRGYL
                     MQQIGIVAKNRWFPLLFTSIVFGLFHSANPEVEQMGYGVMVFYIGTGFLLGIMTLMDE
                     SLELALGFHLGNNLMAALLITSDVSAIHTDALFKYSGVEKPVDMLNEMIVSIAIVYPI
                     ILYIFAKRYNWTNWKTKLTGKIQAPKLQTTN"
     gene            2680..3021
                     /gene="arsC"
                     /locus_tag="PLQ70_07345"
     CDS             2680..3021
                     /gene="arsC"
                     /locus_tag="PLQ70_07345"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480314.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HQE34468.1"
                     /translation="MIQIYHNSRCGKSRECLAFLEDSGKEYEVVKYLENVPTFDELKS
                     IIKKLDIKPIELVRQKEKIWIENFKSKKMSDDEIIQTMILNPILIERPIVINGDKAVI
                     ARPLEKAVAIL"
     gene            3120..5567
                     /locus_tag="PLQ70_07350"
     CDS             3120..5567
                     /locus_tag="PLQ70_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HQE34469.1"
                     /translation="MKNLKITLFVLLILTSITIYSQQKPSFPKIKISGKVIEKVSKQP
                     LEYATISFTDGTSPKSLGGGVTNPKGEFDIDITPGTYDIKIEFISFKPIIIKGKKLQE
                     KTNLGQIALEEDATQLNEVVVRAEKSSVEIKLDKKVYNVGQDMMVKGGTVSDVLDNVP
                     SVSVDTEGNVSLRGSDNIRILIDGRPSYAVNIAEALRQLPADAIDKVEVITNPSARYD
                     AEGGSGIINIILKKGKNQGFNGTVIASTGIPETYGISANVNYKTEKLNYFTTAGYNYR
                     TNEGGGKTNSQYFNDDGSTKNFLDEDRNTERTRDGFNGRAGIEWTVAPNTYWTNAINY
                     QKDNGNNTDLINYKNYDASHNFTGSTYRLGTGESGGENVEFTSNLIKNFNDKGHKLTV
                     DGSISRNTDDSQSIITGSNNYNNTLNNQVQKQAQIQTDYVLPLGEGSQFEAGYKGNFG
                     DLNNEYYVLNDQGVRIENLSNTLEYKEYINALYTQYGFKKNKFSYLFGLRWEDTNIEV
                     NLLDTNDFNTKKYNNFFPSAFVSYEISDQSNITTSYSKRLTRPRGRFMNPAVNYSSNV
                     NIFQGNPDLDPSLTDKFDFGFIKRWDKITFNTSAYFENTKDVFSFVRSPTGDEVNGIP
                     VILSRPINLGREQKYGFEFTLNYSPFKIWKVNSNFNLYNVKTTGENSYTDTQGNLVVQ
                     NLDNQANNWSARVSSKLTLPYKIDWQLNANYNGAQKTAQGRSLDQFGMNTAFSKDLMK
                     DKATIALNVSDIFNTRKMRSYTELENQTSYGEMQFRKRQINLSFTYRFNKAKGEKDKN
                     AQPKNDGGGDGGGEFPG"
     gene            5682..5888
                     /locus_tag="PLQ70_07355"
     CDS             5682..5888
                     /locus_tag="PLQ70_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008257129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34470.1"
                     /translation="MKNSQILVLFLIGAVLTITGALFKITNEPKDWTTFFLIIGMTFE
                     AVAAILMLLKLFKKKNNTDSFLDS"
     gene            complement(5965..7200)
                     /locus_tag="PLQ70_07360"
     CDS             complement(5965..7200)
                     /locus_tag="PLQ70_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQE34471.1"
                     /translation="MLDVGLTTFMILATSLVMLMTPGLAFFYGGLGCNKNILSIMMQS
                     FVSMGIGTVLWFAFGYSLCFSGNMDSGSDFFGIIGNLDKAFYHGITPSTLYSADKRFP
                     EYIFIAYQMMFAIITPALITGAFINRVSFKSYVVFLIFWQIFVYYPFVHMVWGGGLLA
                     EWGVFDFAGGITVHATAGFAALASVYFVGRRQVTHEPNNIPLVAIGTALLWFGWYGFN
                     AGSELAVNSITISSFLNTDTAASFAAVTWLLIEWNTGKKKPTFIGLMTGAVAGLATIT
                     PAAGFVDIYSAALIGIIAAFGCFAAVKFKEKKGWDDALDVWGVHGMGGIIGTICVGFF
                     ANSTINEAIPNGLFFGGDAMLLFKECAAILFATSYAFFFTLLLFKIINKFIPVRVTEE
                     DQELGLDLSHHGEIARQQR"
BASE COUNT         2684 a         1325 c         1115 g         2288 t
ORIGIN      
        1 attcaatatt agaaacttct aataccaatt tttacattat ttcattaaaa attagtaact
       61 tagtgtttaa tttttaaaat taatggatta tgaacaatgt aaaaagtttg aataaatggg
      121 ccaatgcgca cacttattta cctgtagatt tgcttcgaat ggcacttgga attttcttgt
      181 tctggaaagg ggttactttc atcacaaaca tccagtatct agtagattta ttatctccta
      241 tcgacaaatt agatgatgga atgtctggta tgctaatcgt tcattatgtc gcttcggcac
      301 attttttggg aggtatctta attttcttcg gattactgac cagatgggcg ctcatcgtgc
      361 aattacctat tttaattgga gcagttgtta tcaattttat gggtgaaatg cattctcaaa
      421 acttattttt atccctgata actcttgtca tttgcgtggc ttttatcatt tatggtagcg
      481 gaaaacattc agctgactac tatttcaaaa tgcaggaata aatggcaaat aaaaagggag
      541 aatttttgaa aaactctccc ttttttatat tcgataatgc tttatttact tcgataaaat
      601 actttctctt ctcataattt tacccgttgc agtttcttga aacttaggaa taaaaataat
      661 ttcttttggt atttcgtatt tatttaaaag accaaaaaca gattcttcta tttctaatgg
      721 ttcgccttca acataaagaa ttgctttttc gcctaaaacc tcgtctgctt ttccaaaaac
      781 aaaatagcgt cttgggatta aagttgaaag tttttcctct atttgttctg gcaataattt
      841 aattcctcca ctattaataa cattgtcaat tctaccttcc caaataaatt gagtgtccga
      901 aattaatttt acaatatcat tggttacaat tggatcttta ctaatatttt tggctttaat
      961 caccaaacat tgatttttat cctcagaaac agaaacattg ggtaaggttg taaacgctac
     1021 ggcaccaatt tttttggcag caatatgcgt aatggtttca gtcataccgt aggtttcata
     1081 aatttccatt ggcaatttta ctaattcttg ctctagggct ttatgtactc ttacaccacc
     1141 aataattaat tttttaatct ttttttgttt cagtttttcc aaagaatttt tggcttgtaa
     1201 gggaaccatt gcgcaaaaat cgaaacattc gtccattttt tctaatggat ttgaagttgg
     1261 ttcgacaaat ttcatatcta atcctaaaat aaaggaacgc acccacatca tttttccagc
     1321 tacataatta gttggcaaac aatgtaacat agtatctcct ggttgcaaac caaagaaatc
     1381 tcctgttgct aatgctgaat ctaacatggc ttgcttttcg attctgataa ttttaggaac
     1441 accagtagtt ccagacgttt gtaattcgat atacgatttt tcatcaaacc agtctaataa
     1501 gaaatttcca atatgctgtt caaaaggctt accttctttg atgaaagaat aggctacata
     1561 aaacatttct tttcgactaa tatgtattcc gttgagttta aaacggttgt gtatattttt
     1621 atacgttatt ttgtgcattg tgttgtatta atttgtagtt tgtaattttg gagcttgaat
     1681 tttacctgtt agtttagttt tccaattcgt ccaattatat ctttttgcaa agatatataa
     1741 gattatcgga tacacaattg caattgaaac tatcatttca tttaacatat caacaggttt
     1801 ttcaacaccc gaatacttaa aaagagcatc agtatgaatg gctgaaacat ctgaagtaat
     1861 taataaagcc gccatcaaat tgttgcctag atgaaacccc aaagccagtt ccaaactttc
     1921 gtccattaat gtcataatac cgagtaaaaa tcctgtacca atataaaata ccataacgcc
     1981 ataacccatt tgttctacct caggattggc actgtggaac aggccaaaaa ctatggaagt
     2041 gaatagcaac ggaaaccatc tattcttagc aacaatacct atttgttgca tcaaatatcc
     2101 tcgaaataaa tattcttcca atcctatttg acaaggaaat aacaataggc taatggcaaa
     2161 caaaatcgta aatttcaacg gattaaaatt caaaattatg ttagaatgat ctaccaaata
     2221 agagattata aacatcacta ttgttaatga aacaatcaat aaaaaagaga ataaaattct
     2281 tttataatct attgattttc tagaagtagt aagcgaaagc acacttcttt cgtgtaaaac
     2341 acgtaccaac aaaaacaaca atagcaacag taaaataaaa ggcgacaaat tgataagaag
     2401 cgacaaatta ttaggaactt cgctcatcgt tttataaacg gcatccattt gctctttcga
     2461 catcgtaaaa aaagtaacaa aattggcaat aaatactcct gctgtcaaaa gggtggtaat
     2521 taaaacgcgc caccaagtat tatttccttt atatgcttgt tctataaaca tttacaaaaa
     2581 attattaatt taaggattag tttgtttttt gtcagcccga aattaaatta attttccgtt
     2641 tttatacttt actttgcaaa aaaattaaaa acaaatacta tgattcaaat ttatcacaat
     2701 tcgcgttgcg gaaaatcaag agaatgcctt gcttttcttg aagattctgg caaagaatat
     2761 gaagttgtca aatatctcga aaatgttcca acctttgacg aattgaagtc catcatcaaa
     2821 aaattggata taaaacccat tgaattagta cgtcagaaag aaaaaatttg gattgaaaat
     2881 tttaaatcta aaaaaatgtc tgatgatgaa attattcaaa ctatgatttt gaaccccatt
     2941 cttattgaaa ggcctattgt tattaatggc gataaagcag tcatagcaag acctttagaa
     3001 aaagctgtag ctattcttta aaacaattta tctttatttt aacaaacctt taggatttag
     3061 agcttaaacc tatgcccttt tctgaagtct aaattggaaa ttaccaaaaa ctcattttaa
     3121 tgaaaaacct taaaattaca ttatttgtat tgttgatact aacttcaata accatttatt
     3181 cacaacaaaa accttcattc ccaaaaatta agatttctgg aaaagtaatc gaaaaagtaa
     3241 gcaaacaacc attggaatat gctacaattt catttactga tggaacatct cctaaatcac
     3301 ttggaggagg agttaccaat ccaaaaggag aattcgacat tgacatcact ccaggaacat
     3361 atgacattaa aatagaattc atatccttta aacccattat aatcaaaggg aaaaaacttc
     3421 aagaaaaaac aaatttaggg caaatagcct tagaggaaga cgccacacaa ttgaacgaag
     3481 ttgtggttcg tgccgaaaaa tcatctgtcg aaatcaaact ggacaaaaaa gtttataacg
     3541 taggacaaga tatgatggta aaaggcggaa ctgtaagcga tgttcttgac aacgttccat
     3601 cagtttctgt tgataccgaa ggaaatgtta gcctaagagg aagtgataac atccgaatct
     3661 taatcgacgg aagaccttct tatgcagtaa atattgccga agctttgaga caacttcctg
     3721 ctgatgcaat tgataaagtg gaagttatta caaatccatc tgctcgatat gacgccgaag
     3781 ggggttctgg aataatcaac attattctaa aaaaaggtaa aaatcaaggt ttcaacggaa
     3841 ctgtaattgc ttcaaccggt attccagaaa cttatggaat aagtgccaat gtgaattata
     3901 aaacagaaaa attaaactac ttcactaccg ctggttacaa ttacagaaca aatgaaggtg
     3961 gtggtaaaac caattcccaa tatttcaatg atgacggttc tactaaaaac tttctagacg
     4021 aagatcgaaa cacagagaga acaagagatg gttttaacgg aagagctgga attgaatgga
     4081 cagtagctcc taatacctat tggacaaatg ccatcaatta tcaaaaagat aatggtaata
     4141 atactgattt aatcaattac aaaaactatg atgcatctca taattttacg ggttcaactt
     4201 accgtttggg tacaggagaa agtggtggtg aaaatgttga atttacttcc aacttaatca
     4261 aaaacttcaa cgacaaagga cacaaactta cagttgatgg ctccatttca agaaatacag
     4321 atgacagcca aagcattatt acgggttcta acaactacaa taacacctta aataaccaag
     4381 ttcaaaaaca agcccaaata caaaccgatt atgtccttcc gctgggagaa ggaagtcaat
     4441 ttgaagccgg atataaagga aatttcggtg acttaaataa cgaatactac gttcttaacg
     4501 atcaaggagt aagaatagaa aatttgtcaa atactttaga atataaagaa tacattaatg
     4561 cactttatac tcaatatggt ttcaaaaaaa ataaattctc ttatttgttt gggttgcgtt
     4621 gggaagacac taatatcgaa gtaaacttat tggacaccaa cgatttcaat actaaaaaat
     4681 acaacaattt tttcccaagt gcctttgtta gttatgaaat ttcagatcaa agcaacatta
     4741 ccacaagcta cagcaaacgt ttgactagac caagaggacg ttttatgaat ccggcagtaa
     4801 attactctag taacgttaat atttttcaag gaaatcctga cttagatcca tctttaacag
     4861 ataaattcga ttttggattt atcaaacgtt gggacaaaat aactttcaat acttcggcct
     4921 atttcgaaaa cacaaaagat gtttttagct ttgtgagatc ccctacaggt gatgaagtaa
     4981 acggaattcc tgttattttg agccgaccaa ttaacttagg tagagaacaa aaatatggtt
     5041 ttgagtttac attgaattac agcccattca aaatatggaa agtaaacagt aatttcaatt
     5101 tatataatgt taaaacaact ggtgaaaaca gctatacaga tacacaaggc aaccttgttg
     5161 ttcaaaatct tgataaccaa gctaataatt ggtctgcaag agttagttca aaactaactt
     5221 tgccatacaa aattgactgg caattgaatg ctaactacaa tggagctcaa aaaaccgcac
     5281 aaggaagaag cttggatcag tttggtatga atactgcttt cagcaaagat ctaatgaaag
     5341 acaaagctac cattgctctc aatgttagtg atattttcaa cactcgaaaa atgagatctt
     5401 atactgagct tgaaaaccaa acttcttatg gcgaaatgca gttccgtaaa cgacaaatta
     5461 atttatcatt cacctaccgt tttaacaaag caaaaggaga aaaagataaa aacgcccagc
     5521 ctaaaaacga tggcggtgga gatggcggtg gagaatttcc tggataattt taaagcctat
     5581 tcatttcaag aaatgaccta tgaagtaaaa tttcataggt cattttttta taccataact
     5641 tctagttctt ttcaatatct ttgaaacata atttctaaaa aatgaaaaac tcacaaattt
     5701 tagttctgtt cctaattggc gcagtactta ccattacagg cgcattattc aaaatcacaa
     5761 acgaacccaa agattggact actttctttc taatcatagg aatgactttt gaagctgtcg
     5821 ccgcaatatt gatgttgctc aaattattca agaaaaaaaa caataccgat tcgtttctgg
     5881 atagttaatt gtccatccga aaactaaata aaaagggcta cattcattat cgaatgtagc
     5941 ccttttacaa tttataagta atttttatcg ttgttgtctt gctatttcgc cgtgatgcga
     6001 caaatctaat cctaattctt gatcttcttc agtaactctt acaggaataa atttattgat
     6061 tattttaaac aataataaag taaaaaagaa agcataactg gttgcaaata gaatggctgc
     6121 acattcttta aacaacaaca tagcatctcc tccaaaaaac aatccgttcg gaattgcctc
     6181 gtttatagta ctattagcaa agaaacccac acaaatggtt ccaataattc ctcccattcc
     6241 gtgaaccccc caaacatcaa gtgcatcatc ccaacctttc ttttctttga attttaccgc
     6301 agcaaagcaa ccaaaagcag caataattcc aatgagcgcc gcagaataaa tatcaacaaa
     6361 tccagcagct ggagtaatcg ttgctaaccc agcaacagcc cctgtcatta atccaataaa
     6421 agtaggtttc ttttttcctg tattccattc gataagtaac caagtaacag cagcaaaaga
     6481 agccgcagta tctgtattca aaaatgaaga aatagtaatc gaattcactg ccaattcact
     6541 tcctgcatta aatccgtacc aaccaaacca tagcaaagct gtaccaatag caaccaacgg
     6601 aatattattg ggttcgtgag taacttgtcg gcgacccaca aaatataccg aagccaaagc
     6661 ggcaaatcct gcagtggcat gaacagtaat tcctcctgca aagtcaaaaa caccccattc
     6721 agccaataat cctccacccc aaaccatatg aacgaaagga tagtaaacaa atatttgcca
     6781 aaaaattaga aataccacat aggatttgaa tgatactcta ttgataaatg ctccagtaat
     6841 caatgctggt gtaataatgg caaacatcat ttgataagca ataaaaatat attctggaaa
     6901 tcgtttatcc gccgaataca aggtcgaagg agtgattccg tgataaaaag ctttatctag
     6961 attcccgatt attccaaaaa aatcagaacc tgaatccata tttccgctaa aacaaagcga
     7021 atatccaaaa gcaaaccaaa gtaccgttcc tatacccata gaaacaaaac tttgcatcat
     7081 tatacttaaa atgtttttat tacatcctaa tccgccatag aaaaaagcga gtccaggcgt
     7141 cataagcatc accaaactag tggcaagaat cataaaagtg gtcaatccta cgtctaacat
     7201 aattatttta ttaatttcga cacaaaatta actaatcctt tcgcaatatc actaatgttt
     7261 attttacaaa tttcatataa tttgttatgt ttaattaatt ataataggta tcatgccata
     7321 aattgcaata ttattttggt caacccccta aaaaataggg gtgcaatcaa ataaaaaccg
     7381 ttatttattc tggttttaaa ttaataccac aa
//
LOCUS       DAOSNN010000124         7349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10137_length_7349_cov_4.937483, whole genome shotgun sequence.
ACCESSION   DAOSNN010000124 DAOSNN010000000
VERSION     DAOSNN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10137_length_7349_cov_4.937483"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..801)
                     /locus_tag="PLQ70_07365"
     CDS             complement(160..801)
                     /locus_tag="PLQ70_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HQE34472.1"
                     /translation="MKNLFTCLILLCCTTLFSQEIALVKYSGGGDWYANPTSLPNLIK
                     YCNANINTKIKPKPSTVEPSSPDLFSYPFVHMTGHGNVVFSDTDVRNLRNYLTSGGFI
                     HIDDNYGMDQYIRKEIAKLFPNTNLVEIPANHPIFQKPNLFANGLPKIHEHDGKRPQA
                     FGIFIDNRLVLLYTYECDLGDGWEDAEVNNDPLAVREKALKMGANILNYIFNN"
     gene            complement(905..1609)
                     /locus_tag="PLQ70_07370"
     CDS             complement(905..1609)
                     /locus_tag="PLQ70_07370"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HQE34473.1"
                     /translation="MQLFYNPTIDETTETFSFDKEESKHIIKVLRKKDSDILFVTNGF
                     GLLFKTEITLASDNKCTVQILSFEKVEPSKYQLHLAVAPTKMNDRYEWFLEKATEIGI
                     NEITPIICDRSERKVINKERFEKILLTAMKQSNVLFLPKLNDAISFKEFIKRKNDGLN
                     LIAHCEETDKKTLKSVLKTNENVTLLIGPEGDFSEKEIALAVENNFIAVSLGNTRLRT
                     ETAAVVACHSVVFFNE"
     gene            complement(1613..2629)
                     /gene="tsaD"
                     /locus_tag="PLQ70_07375"
     CDS             complement(1613..2629)
                     /gene="tsaD"
                     /locus_tag="PLQ70_07375"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQE34474.1"
                     /translation="MQNSEVFILAIESSCDDTAAAVLQNDKVLSNVVANQLIHNQYGG
                     VVPELASRAHQQNIVPVIDAALRKANITKEQLSAIAFTQGPGLMGSLLVGSSFAKSLS
                     LALNIPLIAVNHMQAHILAHFIDEEGFDKPTFPFLALTISGGHTQIVKVNHFFDMEII
                     GETTDDAVGEAFDKSAKILGLPYPGGPLVDKYAKLGNPKAFVFTKPKVPGLDFSFSGL
                     KTAILYFIQKKKLENPNFIEENINDICASIQHTIIEILMDKLKLAVKETGINQIAIGG
                     GVSANSGIRNTLTENKYGWKTYIPKFEYTTDNAAMIGIVGYQKFLSQNFETSSVVSKA
                     RIQF"
     gene            2845..7260
                     /locus_tag="PLQ70_07380"
     CDS             2845..7260
                     /locus_tag="PLQ70_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQE34475.1"
                     /translation="MTLPFVQTAIGNYVTNAVNEKYKTNINVEQVSLTVFGGVKLKKV
                     LILDHHKDTLIYANRIKTNILDFDKLMNVNLIFGDIAADGLYFNMKTYKKEKISNLDK
                     FVAAFDTGAPPSKEHFLLTATKVKITNGRYSLIDENRENPKDVDFTKINLAATALRIY
                     GPEVSTTVNELSFQDHRGIFVEDLKTKFSYTTKHIKLENLELITKESKVKGSVVMNYE
                     IEDFAHFTDKVAFDIQLEKATLASNDIRCFYDELGKNQLFKIKGRIKGPLNNLVLSNL
                     RLIDSKKSQIVGNINFKNFFPSQGRAFYMNANFDRLSTNYDDLVTILPNVLGKRLPVI
                     LKKLGTINLIGDAQVTTTSLDANFAMSSQIGNLNSDLRIKNMNLTDKAAYDGTVILDD
                     FDIGAFINRKDIGKTSLNLDVDGIGFSEKYLNTTLKGEISKINYNQYTYSNIGLNGNF
                     NKGLYKGQIKANDPNLNLNFDGLVDLSKKQPKYDFHINVENADLKKLNFMKDAISKFK
                     GDIVVKLAGNTIDNLQGDVFVNQVIYQNTKATYRFDDLYINSTFDSNRVRTITVNSPD
                     IVEGEIVGKFQFNQLQNLVTNSLGSLYTNYKPIAVKKGQFLKFNFTIYNKIIEIFYPE
                     ISIATHTVVKGNINSDSREFKLNFNSPKITASTNTFDNIRVKVDNKNPLYNAFIELDS
                     IKTKYYKIRDFSLINVTMKDTLFFRTEFKGGSKGQDYFNLNLYHTINKDNNNVVGLNK
                     SEIKLKDYLWYLNEKETSNNQIVFDKSFKNFNFDNIILTHNEQEITFLGDIKGSTYKD
                     LKLSFKNVDLNQITPSDPKFVINGNLNALVNYKQNVNVFQPTASIQIDKLNINNTNLG
                     NLDFDITGDESLKKFYLNSSIENENVESFGVNGSFEIEDKNTILNLQLKLDKFNLGIL
                     SPLGGEVVSNIRGFATGSASIEGNINKPEINGRIYIDDAGLKIPYLNVDYTLNKRSIV
                     DLTDEKFLFKNDLLTDTKFGTKGYLNGSVEHRNFSDWKLDLGINSKRLLVLDTKDSED
                     AAYYGVAFINGTATIKGPTNALFIKVDAKSEKGSSLKIPINDSESVSENDFLHFLTAK
                     EKFNIKKGIVDNSRNYKGLELEFDLDITPNAEVEVILDRNTGHGMKGKGNGTLLLKIN
                     TLGKFNMWGDFQAYEGEYNFKYGGIIDKKFIVKKGGYISWEGNPMKARLNLEAVYKAS
                     ANPSVLLENSSYNKKIDVNVVIGVRGDLTSPEPDFNFEFPTVSNVMKSEIEYKLNDKE
                     VRQTQALYLLSTGSFLSSDGVSQSALSQSAFETASSLLTSMIHSDDEKFQVNLDIIGA
                     DKTTGQETDGRFVASISSKINDRITINGKVGVPFGGINQTAVVGDVEILYRVNEDGTV
                     NLRFFNRENDISYIGEGIGYTQGVGISYEVDFDTFKELANKIFKNTKIDKAPNSGVID
                     HDSNFAPEKSDPSKSNKPNSKVLNPNSEGRAPEED"
BASE COUNT         2629 a         1081 c         1266 g         2373 t
ORIGIN      
        1 caagaaaaaa gcatcagtac aactgctgcc gataagataa ttttttcata ataaaagtgt
       61 tatataatta gtggtttaag ttaattttca acaattatat aacaaatttg tcaaattggc
      121 aaatatttca cataaaaaac atttacatat ggttgtagtt taattattaa aaatataatt
      181 caaaatatta gcacccattt ttaatgcttt ttcgcgaact gctagaggat cgttgtttac
      241 ttcagcatct tcccatccat cccccaaatc acattcataa gtataaagaa gaaccaatct
      301 attatctata aaaatcccaa aagcttgtgg tcgttttccg tcgtgttcgt gaatttttgg
      361 taaaccattg gcgaacaaat tcggtttttg aaaaatagga tgattggcgg gaatttctac
      421 caaattggta ttgggaaata gtttcgctat ttcttttcta atgtattgat ccattccgta
      481 attgtcatca atatgaataa aacctcccga agtcaagtaa tttctcagat tgcgaacgtc
      541 ggtatcacta aaaactacat ttccgtgacc agtcatgtga acaaaaggat aggaaaataa
      601 gtcgggacta ctaggttcta cagtagatgg tttgggtttt attttggtgt tgatgttggc
      661 gttacagtat tttatcaaat tgggtaatga agtaggattg gcataccaat cgccaccacc
      721 actgtatttt accaaagcaa tttcttggga aaataaagta gtgcaacaca ataaaatcaa
      781 acacgtaaaa agatttttca taaacttctg aaatcaaaaa aaatatttta aacactattt
      841 aaaatctgtt tttatccgtg actttacgaa gtaaatccgt ttcattagcg gaccaattgg
      901 gactttattc attaaaaaaa acaacactat ggcaagcaac tacagctgcc gtttcggttc
      961 ttaatcgtgt atttcctaat gaaaccgcaa tgaaattatt ttcgactgcc aatgcaattt
     1021 ctttttcaga aaaatcgcct tcggggccaa tcaataaagt gacgttttca ttggttttta
     1081 aaactgattt tagagttttt ttatcggttt cctcgcagtg tgcgattaaa tttaaaccat
     1141 cgtttttgcg ttttataaat tccttgaaac tgatagcgtc attcaattta ggaagaaata
     1201 aaacattcga ttgcttcata gccgttagta aaattttctc aaaacgttct ttgttaatca
     1261 cttttctttc agaacgatca catataattg gggttatttc gttaatgcca atttcggtgg
     1321 ctttttctaa aaaccattcg tagcggtcat tcattttggt gggcgcaacg gctaaatgta
     1381 actgatattt tgaaggttct actttttcaa aagaaagaat ttgaaccgtg catttattgt
     1441 ccgacgctaa agtgatttcg gttttgaata gcaagccgaa cccattggtt acaaacaaaa
     1501 tatcggaatc tttttttcgc aatactttga tgatatgttt gctttcttct ttgtcaaaag
     1561 aaaaggtttc ggtggtttca tcaattgttg gattgtaaaa taattgcata gtttagaatt
     1621 gaattcgtgc tttcgaaacc acagaagaag tttcgaaatt ttgggataaa aacttttgat
     1681 aacctacaat tccaatcatt gcagcattat cggtagtgta ttcaaatttt ggaatgtagg
     1741 ttttccaacc gtatttattt tcggtcaaag tgtttcgaat gccagaatta gccgaaactc
     1801 ctccgccaat agcaatttga ttgatgcctg tttctttgac agccaatttc agtttgtcca
     1861 tcaatatttc gataatagta tgttgaatgg aggcacaaat atcattgatg ttttcttcaa
     1921 taaaattcgg attttctagt tttttctttt ggataaaata caaaattgca gttttcaatc
     1981 ccgagaaact gaaatctaat ccgggaactt ttggtttggt aaatacaaat gcttttgggt
     2041 ttccaagctt agcatattta tctactaatg gtccaccagg atagggaagt cctagtattt
     2101 tagcactttt atcgaaagct tctcccacag catcgtcggt ggtttctcca ataatttcca
     2161 tgtcaaaaaa atggtttact ttcacgattt gagtatgtcc acccgaaata gttaaggcca
     2221 aaaaaggaaa tgttggtttg tcaaaccctt cttcgtcaat aaaatgagcc aaaatatgtg
     2281 cttgcatatg atttacagca attagcggga tgtttaaagc caacgataat gacttggcaa
     2341 acgagctccc aaccaataaa gaacccatta atccaggacc ttgagtaaag gcaatggcag
     2401 ataattgctc ttttgttata ttcgctttgc gaagtgcagc atcaataaca ggaacaatgt
     2461 tttgttggtg agcacgagaa gcaagttctg gcacaacacc tccgtattga ttgtgaatca
     2521 attgattagc aacaacattt gacagaactt tatcgttttg taaaacagct gcagcagtgt
     2581 catcgcaaga actttcgata gcaagaataa aaacctcgga attttgcata aaaaggcaca
     2641 aattttgaat tatatttgac aatccgtaat agaaacgtta cggagaagcc aaaattaaag
     2701 aatttttatc aaagagactt tctttaaagg ggcaaatata tttttcattt cattaaaatt
     2761 acagtaggta tcaaaaaaat aattaaaata gtattacgta tccttcttgg acttatccta
     2821 ctattattgg tacttggcat tgcattgaca ctaccttttg ttcaaactgc aattggaaat
     2881 tatgtgacaa atgctgtcaa cgaaaaatat aagacaaaca tcaatgtcga acaagtttcg
     2941 ctaaccgttt ttggtggagt aaaactcaaa aaagtattga ttttagatca tcacaaagat
     3001 actcttattt atgccaatcg tatcaaaacc aatattttag actttgataa attgatgaat
     3061 gtcaatttga tttttggcga tattgcagcc gatggtttgt atttcaatat gaaaacttac
     3121 aaaaaagaaa aaatttccaa tcttgataaa tttgttgccg cttttgatac aggggctcca
     3181 ccatctaaag agcatttttt gttaacggcg accaaagtaa aaattacaaa cggaaggtat
     3241 tctctaattg atgaaaatag agaaaacccg aaagatgtcg atttcacaaa aattaattta
     3301 gccgccacag ccttaagaat ctatggacca gaagtgagta caactgtcaa tgaattatca
     3361 tttcaagatc atcgaggtat ttttgtagaa gatttaaaaa ccaaatttag ttatactaca
     3421 aagcatatta aactcgaaaa tctggaatta attaccaaag aatcaaaagt aaaaggatcg
     3481 gtggtgatga attatgaaat tgaggatttc gctcatttta ccgataaagt agcctttgat
     3541 attcaactcg aaaaagcaac tttggcatcg aatgatattc gttgttttta tgatgaattg
     3601 ggtaaaaatc aactgtttaa aattaaggga agaatcaaag gaccgttaaa taatttggta
     3661 ttatcgaact taagattaat agactctaag aaatctcaaa ttgttggaaa tataaatttt
     3721 aaaaattttt ttccaagtca aggcagagca ttttatatga atgccaactt tgatagactt
     3781 tctacaaatt atgatgattt agtaaccatt ttgcctaatg ttttgggtaa gagattgcct
     3841 gtgattttga aaaaattggg tacaattaat ttgataggcg atgctcaggt tactacaact
     3901 tcattagacg caaattttgc aatgagctct caaattggta atctaaattc tgatttgaga
     3961 ataaaaaaca tgaatttaac agacaaggca gcctatgatg gcacggttat cttagatgat
     4021 tttgacattg gcgctttcat taaccgaaaa gatattggaa aaacaagttt gaatttggat
     4081 gttgatggta tcggtttttc tgaaaaatat ttgaatacaa ctctaaaagg agaaatttct
     4141 aaaataaatt acaaccaata cacttattca aatattggtc tgaacggaaa ttttaataaa
     4201 ggactatata aaggacaaat caaagccaat gatcctaatt tgaatttgaa ttttgacggt
     4261 ttggtcgatt tgagtaaaaa acaaccaaaa tatgattttc atatcaatgt tgaaaatgct
     4321 gatttgaaaa aattaaattt catgaaagat gctatttcca aattcaaagg agatattgtg
     4381 gtgaaattag caggaaatac aatagacaat ctacaaggtg atgtgtttgt taatcaagtc
     4441 atttatcaaa acacaaaagc aacgtatcgt tttgatgatt tatatatcaa ttctactttt
     4501 gattcaaacc gtgtgagaac cattacagta aactctcccg atatcgttga aggtgaaatt
     4561 gttggaaaat tccaattcaa tcaattgcaa aatttagtta ccaattcact aggaagtctt
     4621 tataccaatt acaaacctat cgcagtaaaa aaagggcagt ttttgaaatt taattttaca
     4681 atttataata aaataatcga aattttttat ccagaaattt ctattgctac ccatacagtc
     4741 gtaaagggaa atattaactc agatagtcga gaatttaagc tcaattttaa ttcgccaaaa
     4801 atcacggctt cgaccaatac tttcgataat attagggtga aagttgacaa taaaaacccg
     4861 ttgtataatg cttttattga acttgatagc atcaaaacta agtattataa aatacgagat
     4921 tttagtttga taaacgtaac aatgaaagat actttattct ttcgtacaga gtttaaggga
     4981 ggctcaaaag gtcaagatta ctttaatctg aatttgtatc acacaataaa taaagacaat
     5041 aataatgtag ttggactaaa taaatccgaa ataaaactta aagattatct ctggtatttg
     5101 aatgaaaaag agacatctaa taatcaaatt gtttttgata aatcattcaa gaatttcaat
     5161 tttgacaata ttatactaac tcataatgaa caagaaatta cctttttggg tgatataaaa
     5221 ggaagtacgt ataaagattt gaaattgagt tttaaaaatg ttgatttaaa tcaaattact
     5281 ccatctgatc cgaaatttgt catcaatgga aatctgaatg cattagtcaa ttataaacag
     5341 aatgtaaatg tttttcagcc cacagcttct attcaaatag acaaattaaa tattaataat
     5401 accaatttag ggaatttgga ttttgatatt acaggagatg aaagtttaaa aaaattctat
     5461 cttaactcct caatcgaaaa tgaaaatgta gagtctttcg gggttaatgg aagttttgaa
     5521 atagaagata aaaataccat tttgaacttg cagttaaaat tagacaagtt caatctgggg
     5581 attttaagcc cgcttggcgg tgaagtcgtt tcgaatatta gaggttttgc aacagggagt
     5641 gccagtattg aaggcaatat taataagccc gaaattaatg ggcgtattta tatagatgat
     5701 gcgggattaa aaattcctta tttgaatgtt gattatacct taaataaaag gtctattgta
     5761 gatttgacag acgagaagtt tttgtttaaa aacgatctgc ttaccgatac aaaatttgga
     5821 accaaagggt atttgaacgg aagtgtagaa caccgtaatt tttcagattg gaaattggat
     5881 ttaggaatta attcaaagcg attattagta cttgacacca aagattctga agacgctgct
     5941 tattatggag ttgcctttat taatggaaca gcaaccatca aaggcccaac aaatgctttg
     6001 tttattaaag ttgatgcaaa atctgaaaaa ggatcgtcat tgaaaatccc tataaatgat
     6061 tcggaaagtg tcagcgaaaa tgatttttta cattttttaa cggccaaaga aaagtttaat
     6121 attaaaaaag gaattgtaga taattctaga aattacaaag ggcttgaact agagtttgac
     6181 ttagatatta caccaaatgc cgaagtagaa gttattttag atcgaaacac aggtcatgga
     6241 atgaaaggaa aaggaaatgg aaccttactt cttaaaataa acacattagg aaagtttaat
     6301 atgtggggag attttcaagc ctatgaagga gagtataatt ttaaatatgg aggtataatc
     6361 gacaaaaaat ttatagtaaa aaaaggaggc tatatttctt gggaaggcaa tccgatgaaa
     6421 gcccgtttga atttagaagc tgtttataaa gcctctgcta atccctctgt tttgctagaa
     6481 aatagttctt ataataaaaa gattgatgta aatgttgtta taggtgttcg aggagatttg
     6541 acaagcccag aacctgattt taattttgaa tttccaacgg tgagtaatgt gatgaaatcc
     6601 gaaatagaat ataaattaaa tgacaaagaa gtaagacaaa cacaagcttt atatttgtta
     6661 tctacaggaa gttttttaag ttccgatggg gtgagtcaat ccgcattatc gcaaagtgca
     6721 tttgaaacgg cttcaagtct attaacaagt atgatacact ctgatgacga aaaatttcaa
     6781 gtaaatttag atattattgg agccgataaa actacaggac aagaaactga cggaagattt
     6841 gtagcttcaa tttcttctaa aattaatgac agaattacca taaacggaaa agtcggagtc
     6901 ccttttgggg gaataaatca aacagcggtc gttggcgatg ttgaaatttt atatagagtt
     6961 aatgaagatg gaaccgtaaa tctgcgcttt tttaatagag aaaacgacat tagttatata
     7021 ggagaaggaa ttggttatac tcagggagtt ggtatttcat acgaagtaga ttttgatact
     7081 ttcaaagagt tagctaataa aatatttaaa aacacaaaaa tagataaagc tccaaattca
     7141 ggagtaattg atcacgattc caattttgca cctgaaaaaa gtgacccatc caaatcgaat
     7201 aaacccaatt cgaaggtgct aaatcccaac tcagaagggc gagctccaga ggaagactaa
     7261 actcattttg aattcgcata aattagtaag ttaataaaaa tagtattgca ttaaattaac
     7321 gtttctcaag gcaattatta tttttatta
//
LOCUS       DAOSNN010000125         7314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10188_length_7314_cov_5.382009, whole genome shotgun sequence.
ACCESSION   DAOSNN010000125 DAOSNN010000000
VERSION     DAOSNN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10188_length_7314_cov_5.382009"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..142)
                     /gene="prfB"
                     /locus_tag="PLQ70_07385"
                     /pseudo
     CDS             complement(1..142)
                     /gene="prfB"
                     /locus_tag="PLQ70_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986097.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            230..1249
                     /locus_tag="PLQ70_07390"
     CDS             230..1249
                     /locus_tag="PLQ70_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HQE34476.1"
                     /translation="MRINLISDTVTKPTQEMLRAMFRADVGDDVYKQDPTVIELEARV
                     AEMFGMEAGLFFPSGTMANQTAIKLHTQPGEQLIADKWAHVYNYEGGGASFNSGVSCC
                     LLDGNRGMITAEQVACAINDPEFYHSPLTSLVCVENTTNKGGGACYDFEEFKKIRQVC
                     DANNLKFHLDGARIWNALVATNDNPLAYGKLFDTISVCLSKGLGAPIGSVLISDKATI
                     HRALRIRKILGGGMRQVGYLAAAGLYALENNIGRLAEDHRRAKEIGAVLEKLNWVAKV
                     EPIETNILIFSLHPQISEAVLLEKLHQKGIGISSMGQGKLRIVTHLDYREVMHSYVLE
                     TLGKL"
     gene            complement(1293..1613)
                     /locus_tag="PLQ70_07395"
     CDS             complement(1293..1613)
                     /locus_tag="PLQ70_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQE34477.1"
                     /translation="MDLMGMMGKLKETQRKMEETKLRMDTVLIDEQSNDGALKVTFTA
                     NGKLKSIAIADSLLEDKEQLEDYLIVTLNKAIEKAAKVNQAELDAVAKVDMPMIPGMD
                     EMFK"
     gene            1755..3815
                     /locus_tag="PLQ70_07400"
     CDS             1755..3815
                     /locus_tag="PLQ70_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQE34478.1"
                     /translation="MSKKIQPPIAKIIPKKLEKFGDVRIDNYYWLNDRENPEVIDYLN
                     KENAYYKKMTAHTKAFQKELFEEMKSRIKEDDESVPYLYNGYYYITRFEKGKNYPIYS
                     RKKESLSAKEEILFDCNKLAKGQSYFQLGGLSISPDNKLAVFSTDVVGRRIYDIQIKN
                     LETGKILSDKIEKVSGNAVWANDNKTIFYSSQDEVTLRSDKIFKHKLGTTQADDVLVY
                     FEKDETYNVEVAKSKSRKYLAIESGSTLTTEYQILEADNPDGKFRVFQKRIRGLEYSI
                     NHYGDSFYIMTNAEKATNFKLMKTLETATSKENWTEVISHRDNVLLEDIEIFKDFLVL
                     EERTNGLNKIRIMPWSGEGEYYLPFDSETYTAYTSTNVDFDTDILRYSYQSMTTPSSV
                     IDFNMRTKIKEIKKEQQVLGGKFDKNNYTEERIWATAKDGTKVPISVVYRNGLEKNGK
                     NPLLLYAYGSYGHSMDATFSSTRLSLLDRGFVFAIAHIRGGEDLGRQWYEDGKLLKKK
                     NTFTDFIDCSKFVIEQGYTSSEHLYAEGGSAGGLLMGAIVNMAPELYHGIIAQVPFVD
                     VITTMLDESIPLTTGEYDEWGNPNVKKYYKYMASYSPYDNVKKQKYPNMYVSTGLHDS
                     QVQYWEPAKWVAKLRSEKADDTVLFLDTNMDAGHGGASGRFEAIKELAKEYCFLLDLE
                     KIEK"
     gene            3888..4928
                     /locus_tag="PLQ70_07405"
     CDS             3888..4928
                     /locus_tag="PLQ70_07405"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HQE34479.1"
                     /translation="MKEEIKAYNNILELIGNTPLIKLNKVTQELKGNFYAKVEAFNPG
                     HSTKDRIALFIIEEAERRGILTPGDTIIETTSGNTGFSLAMVSIIKGYNCILAVTSKS
                     SKDKIDMLRALGAKVYVCPAHVSADDERSYYNVAKRLHEETKGSVYINQYFNQLNVDA
                     HYKSTGPEIWNQTNGKITHLIACSGTGGTISGTAKFLKEKNPNIRILGVDAFGSVLKK
                     YHETREFDNSEIYPYRIEGLGKNLIPSATDFDVIDKFMKVTDEESAHSTRELAKTEGL
                     FVGYTSGAVLQAIKQYAEEGEFDENSNIIAIFPDHGSRYMSKVFSDDWMNEQGFFDSI
                     NEEEAQKIEFIK"
     gene            complement(4942..5763)
                     /gene="rluF"
                     /locus_tag="PLQ70_07410"
     CDS             complement(4942..5763)
                     /gene="rluF"
                     /locus_tag="PLQ70_07410"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HQE34480.1"
                     /translation="MEENLKRLNKFIGETGFCSRREADKIIEEGRVTINGVVPELGTK
                     VAPDDEVRIDGKLIRVKRAKSIYLAFNKPVGIECTTNLDVRNNIVDYINYPTRIFPIG
                     RLDKASEGLIFMTDDGDIVNKILRARNNHEKEYTVTVNKLITDRFIEKMSNGVPILDT
                     VTRKCKVEKISSTTFKIILTQGLNRQIRRMCEYLGYEVIALKRIRIINISLDIPVGRY
                     RDLTEAEIKELNQLIEPSSKTEEASLPKPEKTEIPKKRTEFIKRDDPRFKRRGNY"
     gene            complement(5911..6753)
                     /locus_tag="PLQ70_07415"
     CDS             complement(5911..6753)
                     /locus_tag="PLQ70_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQE34481.1"
                     /translation="MKKTFYFLLTKSIGSYINLMSFIFPEKAIQMAHGYFSEPRKGKI
                     TQNKLPKTLQGAHSETIFHNEDVIQTYIWKGNDTVILLIHGWESNSSRWKKMLPYLTK
                     SGSTIVAIDGPAQGLSSGKEFTVPKYAEFMDIVAKKYQPNFIIGHSMGGQTCLYYQYK
                     YQNPNIRKMVILGAPSDFTIILNNFTNLLSINSKVTKALENKYTHFLNLDLSQFSSKE
                     FASKIETKGLLAHDIDDTVVLFKEAKKIAESWKDVQFIETKGLGHKLHDDELYQKINK
                     FLFE"
     gene            complement(6863..>7314)
                     /locus_tag="PLQ70_07420"
     CDS             complement(6863..>7314)
                     /locus_tag="PLQ70_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34482.1"
                     /translation="EIQLAEKQIKEMKASIEHKKAIIAESKERLEIKSNHLKHKKSEL
                     DDIMSETAKEETFLSDKSFEYRASIEPRLLAAYDRIRSSVRNGLAVVSIERGASAGSF
                     FTIPPQTQVEIAARKKIITDEHSGRILVDSVLADEEKEKMEQLFAKF"
BASE COUNT         2379 a         1137 c         1364 g         2434 t
ORIGIN      
        1 tttggcatta ttccaaaaat ctggagctag tgttttctct tcttcgttag taatttcaat
       61 taatttggca tcgacgtcaa agatacctcc tcaacgcacc aaggcgctct acaatacctt
      121 ttatttgttc ggtagttgtc ataaattata gtaattttgt ggcacaaaaa tagaaaattt
      181 tatgtagagc cctaatccgg tagggctatt tttaaatata aattcaaaaa tgcgaataaa
      241 tttaattagt gatactgtaa ctaaaccaac tcaggaaatg ttgcgggcca tgtttcgagc
      301 agatgtagga gatgatgttt acaaacaaga tccaacagtc atcgaattgg aagccagagt
      361 tgccgaaatg tttggaatgg aagcaggtct tttttttcct tcaggaacga tggccaatca
      421 aacggcaatc aagctacata ctcaacccgg agagcaatta attgctgata aatgggctca
      481 cgtttacaat tatgaaggcg gtggagcttc ttttaatagt ggcgtttcct gctgtttatt
      541 ggacggaaat cgcggaatga ttacggccga acaagtggct tgtgccatca atgatccaga
      601 attttatcac agcccgctta cgagtttggt ttgtgttgaa aataccacta ataaaggagg
      661 tggtgcttgt tatgattttg aagaattcaa gaaaatccgc caagtttgtg atgccaataa
      721 tttaaaattt catttagatg gtgcccgaat ttggaatgct ttggttgcta ctaatgacaa
      781 tccgttggca tacggaaaac tatttgatac tatatcggtt tgtttgtcta aaggtttggg
      841 agcaccaata ggttcagtat taatttctga taaagccact attcacagag ctttgcgaat
      901 taggaaaata ttaggtggag gaatgcgaca agtaggttat ttggcggcag ctggacttta
      961 tgctttggaa aataatatcg gaagattagc agaagatcac aggcgtgcca aagaaatagg
     1021 tgctgtttta gaaaaattga attgggttgc caaagtagaa cccattgaaa ctaatatttt
     1081 aattttttcg ttgcatccgc aaattagcga agcagtttta ttagaaaaat tacatcaaaa
     1141 aggaattggg ataagctcga tgggacaagg aaaactccga attgtcaccc atctggatta
     1201 tcgcgaagtg atgcattctt atgtattgga aaccttaggg aaattatgag ttattaatta
     1261 ttagttgaat aattcataac tcataattta aattatttaa acatttcatc cattcctgga
     1321 atcattggca tatcgacttt tgcgacagca tctaattcag cttggtttac ttttgctgct
     1381 ttttcgatgg ctttgtttaa ggttacaatt aaataatctt ctagttgttc tttgtcttct
     1441 aataatgaat cagcaatcgc aatcgatttt aattttccgt ttgctgtaaa ggttactttc
     1501 aatgcgccgt cattgctttg ttcgtctatc aaaacagtat ccatacgcag ttttgtttct
     1561 tccatttttc gttgggtttc tttcaattta cccatcattc ccattaaatc cattgttata
     1621 attttaaaag tttaatgcaa aattactctt ttgtagcttg atattcaatt taatttttta
     1681 actaaaaata ggctcgtttt atagtggttt gtgtattttt gtggttaaca caatataaat
     1741 agaaagattg caatatgtct aaaaaaattc aaccaccaat cgctaaaata atccctaaaa
     1801 aattagaaaa gttcggcgat gtacgaattg ataattatta ttggttgaat gatcgagaaa
     1861 atccagaagt tattgattat ctgaacaaag agaatgccta ttataaaaaa atgacggctc
     1921 ataccaaagc atttcagaag gaattatttg aagaaatgaa gagcagaatc aaggaagatg
     1981 atgagtcggt gccgtatttg tataacggtt attattacat cacccgattc gaaaaaggaa
     2041 aaaattaccc tatttattcc cgaaaaaaag aaagcctttc ggccaaagaa gaaattttat
     2101 tcgattgcaa taaattagcc aaaggacaat cttattttca attaggtggt ttgagtatta
     2161 gtcccgacaa taaattggct gtttttagta cggatgttgt tgggcgtaga atttatgata
     2221 ttcaaatcaa aaatttagaa acgggtaaaa ttttatccga taaaatcgaa aaagtctctg
     2281 gaaatgcagt ttgggcaaat gataataaga ctatttttta ttccagtcaa gacgaagtga
     2341 ctttgcgttc tgataaaatt ttcaagcata aattaggaac aactcaggct gatgatgtat
     2401 tagtttattt tgaaaaggac gaaacgtata atgttgaggt tgctaaatcg aagtctagaa
     2461 aatatcttgc gatagagtcg ggaagtacat taactacgga atatcaaata ttagaagctg
     2521 ataatccaga tggaaaattt agagtttttc aaaaaagaat tcgaggtttg gaatacagta
     2581 tcaatcatta tggtgatagt ttttacatca tgaccaatgc agagaaagcc acgaatttta
     2641 agttgatgaa aactttagaa acagcaactt ctaaagagaa ttggacagaa gttatatcgc
     2701 atagggacaa tgttttatta gaagatattg agatttttaa agattttttg gtgctagaag
     2761 agcgtactaa tggtttgaat aaaattagaa ttatgccttg gagcggggag ggagaatact
     2821 atttgccttt tgatagtgaa acttatactg cttatacttc aacaaatgta gattttgaca
     2881 ctgatatttt gcgttattcc tatcaatcga tgacaactcc ttcttctgtg attgatttta
     2941 acatgagaac taaaatcaag gaaatcaaga aggagcaaca ggttttagga ggtaaatttg
     3001 ataaaaataa ttataccgaa gagcgaattt gggctacggc aaaagatgga acgaaagtac
     3061 caatttcggt agtttacaga aacggattgg agaaaaacgg aaagaatccg ttgttgttat
     3121 atgcctatgg ttcttacgga cattcgatgg atgctacttt ttcatctaca cgattgtctt
     3181 tattagacag aggatttgtt tttgctatag cacacatccg cggaggcgaa gatttgggaa
     3241 ggcaatggta tgaagacggt aaattgttaa aaaagaaaaa tacgtttact gattttattg
     3301 actgttctaa atttgtcatt gaacaaggat atacttcgtc tgaacatttg tatgccgaag
     3361 gtggttcggc aggaggattg ctgatgggag caattgtaaa tatggctccc gaattgtatc
     3421 acggaatcat cgctcaagta ccttttgtag atgttataac cacaatgttg gacgaaagca
     3481 tcccacttac gacaggagaa tatgacgaat gggggaatcc aaatgttaaa aaatattaca
     3541 agtatatggc ttcttattca ccttatgata atgttaaaaa acagaagtat cccaatatgt
     3601 atgtttcaac aggattgcat gattctcagg tacagtactg ggaaccagct aaatgggtgg
     3661 ctaagttgcg aagtgaaaaa gcagatgata ctgttttatt tctcgatacc aatatggatg
     3721 caggtcacgg aggagcttct ggaaggtttg aagcgattaa ggaattagca aaagaatatt
     3781 gttttttatt agatttagaa aaaattgaaa agtaattaga ttatttttgt taaatttgca
     3841 gggatttgga gttgaataaa aaagagtcct tgattaacta tttttttatg aaagaagaaa
     3901 taaaagctta taataatatt ttagaattaa taggcaacac accgctcatt aaattaaata
     3961 aagttaccca agaattaaaa ggaaattttt acgctaaagt agaagctttt aatcccggtc
     4021 attccaccaa agatagaata gctctattca ttattgaaga agccgaaaga agaggaattc
     4081 ttactccagg agatacaata atagaaacta cttcaggaaa tactggtttt agtttagcaa
     4141 tggttagtat catcaaagga tacaattgta ttttggctgt tacttccaaa tcttctaaag
     4201 acaaaatcga tatgttgcgt gccttaggag caaaagttta tgtttgtcct gcacacgttt
     4261 ctgccgatga tgaacgttcc tattacaatg ttgccaaacg tttacacgaa gaaactaaag
     4321 gatctgttta tatcaatcaa tacttcaatc agcttaatgt tgatgcacat tacaaatcta
     4381 ctggacctga aatttggaat caaactaatg gtaaaattac ccatcttatt gcttgtagcg
     4441 ggacaggagg aaccatttct gggacggcaa aatttttaaa agagaaaaat ccaaatatta
     4501 gaattttagg cgttgatgct tttggttctg tattaaaaaa ataccacgaa actagagaat
     4561 ttgataatag cgaaatctac ccgtacagaa ttgaaggctt aggaaaaaat ttgattcctt
     4621 ctgcaacaga ttttgacgtt atcgacaagt ttatgaaagt taccgatgaa gaaagtgcac
     4681 attctactag agaattagcc aaaacggaag gtttattcgt gggttataca tctggagccg
     4741 ttcttcaagc tataaaacag tatgccgaag aaggagagtt tgatgaaaat agtaatataa
     4801 ttgcaatttt cccagatcat ggttctcgtt atatgagtaa agtgtttagt gatgactgga
     4861 tgaacgaaca aggatttttt gatagtatca acgaagaaga agctcaaaaa attgaattta
     4921 taaaataatt cttaattaat tctaataatt gcctcttctc ttaaaccttg gatcatctct
     4981 tttgatgaat tcggttcttt ttttaggtat ttctgttttc tcaggttttg gtaaacttgc
     5041 ttcttcggtt ttactcgaag gttcgataag ttgattcaat tccttgattt ctgcttcggt
     5101 taaatcacga tatcttccta ctggaatatc caatgaaata ttgatgattc gaatgcgttt
     5161 caaagcaata acttcataac ccaaatactc acacattcta cggatttgac ggttcaaacc
     5221 ttgggtcaat atgattttaa aggtcgtcga actgattttt tctaccttgc attttcgagt
     5281 aaccgtgtcc aaaattggaa cgccattgct cattttttcg ataaaacgat cggtaataag
     5341 tttgttaacc gttacggtat attctttttc gtgattgttt ctggcacgaa gaattttgtt
     5401 gactatatcg ccatcatcgg tcataaaaat caagccttca ctggctttgt ctaatcgtcc
     5461 aatcgggaaa attcgggttg gataattgat gtaatctaca atattattac gaacatctaa
     5521 attggtcgta cattcaattc ccacaggttt gttgaaagcc agatagattg atttggctct
     5581 tttcactcga attaatttgc cgtcaattcg cacttcgtcg tctggcgcaa ctttagttcc
     5641 tagttctgga acaaccccat tgatagttac tcgaccttct tcaatgattt tatccgcttc
     5701 acgacgcgaa caaaaaccag tttctccaat gaatttattg agacgtttta gattttcttc
     5761 catagtgcaa aagtaaacaa tttgagtacg taaaaacagt tattacccag agattcacaa
     5821 agtaaatcgc agagatactc aaagtaaaat tattaaaacc ttagcgaaac tttgcgtttt
     5881 cttagtgaat ctctgtgtag aaaatagtag ctattcaaac aaaaacttat tgattttttg
     5941 atacaattca tcatcgtgaa gtttgtgtcc caaaccttta gtttcaataa attgaacatc
     6001 tttccaagat tcagctattt tttttgcttc tttaaataat accacggtgt catcaatatc
     6061 gtgagcaaga agaccttttg tttctatttt agaagcaaat tctttggatg aaaattgaga
     6121 taaatccaga ttcaaaaaat gggtgtattt gttttctaac gctttggtta ctttactatt
     6181 tatacttaat aaattggtga agttgtttag aataatagta aaatcacttg gagcacctag
     6241 aattaccatt tttcgaatat tgggattttg gtatttgtat tgatagtaca aacaggtttg
     6301 ccctcccatt gaatggccaa ttataaaatt tggttggtat ttcttggcaa caatatccat
     6361 aaactcggca tattttggaa cggtaaattc tttgccacta gacaatccct gagcaggacc
     6421 atcgatggca actattgtgc ttcctgattt tgttaaataa ggaagcattt ttttccatct
     6481 agaggaatta ctttcccaac cgtgaataag caaaattaca gtgtcatttc ccttccaaat
     6541 ataggtttga attacatctt cgttatgaaa gattgtttct gaatgagctc cttgtaaggt
     6601 tttaggaagt ttgttttggg tgatttttcc ttttctaggt tcactaaaat agccgtgagc
     6661 catttgaata gctttttctg gaaatataaa actcattagg ttgatgtaag acccaatcga
     6721 tttggttagc aaaaaataaa atgttttttt cataaacaaa ataatatgat tacgacccta
     6781 ttgccttaaa aaaataaagt ccgcatagcg gactaaatat agtataaaaa aagtcccgat
     6841 ttgtatcggg acttaactat ttttagaatt tagcaaaaag ctgttccatt ttttctttct
     6901 cttcatcggc caatacactg tcaaccagga ttcttccaga atgttcatca gtgatgattt
     6961 tttttctagc agcaatctct acttgagttt gtggcggaat agtgaaaaat gatcctgcag
     7021 aagctcctct ttcgatagaa acaacagcca atccgttgcg aacacttgaa cgaatacgat
     7081 cataagctgc tagtaaacga ggttcgatag aggctctata ttcaaatgat ttatcggaca
     7141 agaatgtttc ttcttttgca gtttcagaca taatatcgtc taattctgat tttttatgtt
     7201 ttaagtgatt cgatttaatc tctaatctct cttttgattc tgcaataatt gcttttttat
     7261 gttcgataga agctttcatt tctttgattt gcttctcggc caattgaatt tcta
//
LOCUS       DAOSNN010000126         7228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10359_length_7228_cov_4.661927, whole genome shotgun sequence.
ACCESSION   DAOSNN010000126 DAOSNN010000000
VERSION     DAOSNN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10359_length_7228_cov_4.661927"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLQ70_07425"
     CDS             complement(<1..891)
                     /locus_tag="PLQ70_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34483.1"
                     /translation="MNIKHTIFVLLISLSVNTLFAQKDGYWDKERATKKEIIVSARDR
                     FIIKTEELPVGTTEVVYRITLLNQNQEMANSLVSVLKSIPDPTGISQGSAGAVFLLSK
                     ISGEDKCKYAIFSSEISASDYQKNGTTTKACYEQNVPVSQDAKGLSVDKSLCILPNSN
                     AMWFGFESKNWIMNQKIVLEVVPWVNTRLSRGWNLENRKLILNQCKTTDLAKKIIPSD
                     DFCVCVLDKLQKEYKFQEFQKLLTVEKSKAYTDFGNSCLKEIGTSDKIYSDLRNQAAN
                     LVQQGKYAEAIDKIGIIVIYG"
     gene            complement(1071..1358)
                     /locus_tag="PLQ70_07430"
                     /pseudo
     CDS             complement(1071..1358)
                     /locus_tag="PLQ70_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(1489..2166)
                     /locus_tag="PLQ70_07435"
     CDS             complement(1489..2166)
                     /locus_tag="PLQ70_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HQE34484.1"
                     /translation="MKITFYGHGSLGIQVGGKHILVDPYISANPNASHININELTADY
                     ILLTHAHGDHVLDVEYIAGRTNAVIVSNYEIATHYAEKGLKSHPMNHGGSWNFDFGKV
                     KYVTAIHSSSFPDGSNGGNPGGFVIEGEHKNIYIAGDTSLTMDMKLIPMRTKLDLAIL
                     PIGSNFTMDVEDAIIASDFIECDKILGAHYDTFGYIEINHEEAIRNFFDKGKDLMLLE
                     IGESIEL"
     gene            complement(2235..3197)
                     /gene="menA"
                     /locus_tag="PLQ70_07440"
     CDS             complement(2235..3197)
                     /gene="menA"
                     /locus_tag="PLQ70_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HQE34485.1"
                     /translation="MKHWIQAARLRTLPLSVSGIVVGSMYALAHPTDFELTPTEVASW
                     TIFGFAILTTLGLQILSNFANDYGDGMKGTDNEDRIGPKRAIQSGLISPQAMKRALVI
                     TSFLTLISAILLIYFAFKDTNIGYSLFYLVLGILAIASAIRYTVGNTAYGYRGFGDLF
                     VFIFFGLVSTIGVNFLYTKEVEWPLILPATAIGFLSVGVLNLNNMRDEASDRKSNKNT
                     IVVKIGGEKAKKYHYFLIVSAMVLVLVFAILNDFNLDQYLFLLAYIPLTKHLITVYKN
                     QEPRALDPELKKLALSTFALSMLLAICMISLIQDLIVNLFLGGR"
     gene            complement(3327..4208)
                     /locus_tag="PLQ70_07445"
     CDS             complement(3327..4208)
                     /locus_tag="PLQ70_07445"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007651228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HQE34486.1"
                     /translation="MNLKNELFLHLYFKPNKMDWITVKEYEDITYKKCNGVARIAFNR
                     PNVRNAFRPKTTSELYQAFYDAQEDTSIGVVLLSAEGPSTKDGVYSFCSGGDQNARGH
                     QGYVGDDGQHRLNILEVQRLIRFMPKVVIAVVPGWAVGGGHSLHVVCDLTLASKEHAI
                     FKQTDADVTSFDAGYGSAYLAKMVGQKKAREIFFLGRNYSAQEAMEMGMVNAVIPHDE
                     LEDTAYQWAQEIMEKSPTSIKMLKFAMNLTDDGMVGQQVFAGEATRLAYMTEEAKEGR
                     NAFLEKRKPNFEKKWLP"
     gene            4855..5709
                     /locus_tag="PLQ70_07450"
     CDS             4855..5709
                     /locus_tag="PLQ70_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HQE34487.1"
                     /translation="MTPKKAFFSIALLFFIYTSTLAQQLVSNDLTVEVTPEFVDSTAV
                     NLDKTTLVLNDSLTAINDSVAPIVQNVPAAYPPPYRDYRRLGYDTALFAGAAVVGFGV
                     LWVMPESVTNWDKEEMRDKGVFWKWKENVKAGPVWDKDDWVLNWITHPYCGGIYYMTA
                     RSSGFTALESFGYSAIMSTFFWEYGIEAFAEVPSIQDLIITPVVGSVVGEGFFYAKKS
                     IVRHDKKVLKSRFLGITTLFLMDPFNTILDGFGYKEKVKTQMNIAPVGVNQFSKTPVW
                     GVSFSAIF"
     gene            complement(5816..6679)
                     /locus_tag="PLQ70_07455"
     CDS             complement(5816..6679)
                     /locus_tag="PLQ70_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HQE34488.1"
                     /translation="MIEIGKYNTLTILRDTKVGLFLGNPEKDPEGIHDILLPNKYVPN
                     EWEIGEEIIVFVYLDHEERPVATTLEPYILLNEFALLRVNYVNQVGAFMDWGMEKDIL
                     VPFKEQARPMEKGKRYLVYLYMDEKTKRLVASSKTNQFLKNDHLTVEKGEEVDLIVSH
                     ITELGINVIINERHKGLLYKDEVYDDSIRTGDRLRGYIKTIRPDHKIDVSLQIQGYQN
                     IEPNAEKILDELRASRGFLRLTDNSHPEDIKTVLKMSKKTFKKAIGALYKEKLIEIKE
                     DGIYLVKEKAL"
     gene            complement(6753..7097)
                     /locus_tag="PLQ70_07460"
     CDS             complement(6753..7097)
                     /locus_tag="PLQ70_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HQE34489.1"
                     /translation="MKTEKYKIYFLHILEAIEKLELVATTTTKEEFLNNWLKQDAVLK
                     NFIVIGEAISQIDEEIKLKFPDVDWRGAKSMRNFIVHEYFSVDNSFVWETIFETIPTF
                     KIQIEKILKELE"
BASE COUNT         2273 a         1272 c         1184 g         2499 t
ORIGIN      
        1 tccgtaaatc acaataatcc caattttatc aattgcttcg gcatattttc cttgttggac
       61 taaattggca gcttgattgc gtaaatccga atatatttta tctgaagttc ctatttcttt
      121 taaacaagaa ttaccaaaat cagtataggc tttcgatttt tcgacagtca atagtttttg
      181 gaattcctga aatttgtatt ctttttgtaa cttatcaagc acacaaacac aaaaatcgtc
      241 agaaggaata attttcttag ccaaatcagt ggttttacat tgattgagaa tcaatttccg
      301 gttttcgaga ttccaaccgc gacttaatct agtattgacc caaggcacta cttctagaac
      361 tattttttgg ttcattatcc agtttttgct ttcaaaacca aaccacattg cattagaatt
      421 gggtaaaata cacaaagatt tatcaacaga aagtcctttg gcatcttggc taacgggaac
      481 attttgttca tagcaagctt ttgtagttgt tccgtttttt tggtaatctg aagccgaaat
      541 ttcactcgaa aaaatagcgt atttgcattt gtcttcgcct gaaattttgg aaagtagaaa
      601 aactgctcca gccgaacctt gactgattcc tgttgggtcg ggaatggatt ttaaaacaga
      661 aaccaaacta ttggccattt cttgattctg attcaagaga gtaattcgat aaaccacttc
      721 ggtggttcct actggcaatt cttcagtttt aataataaat ctatctcttg ccgaaactat
      781 aatttctttt ttggttgctc gttccttatc ccaataaccg tctttttgag cgaaaagggt
      841 gttaacagaa aggctaatta gtagaacgaa aatggtgtgt tttatattca tttattaagt
      901 gtaaatacgc tataattatt taaattttag ttgtctattc gaatgattat caattattaa
      961 atttacccgt ctcttcgagt gattttgaat agtaatgcga cagctttact attcaaaatt
     1021 gtatcgagaa gctttataaa tcgcaaatat tctctaagca ttgaacatct ttaaaacttt
     1081 ttctttgata aattaggcag tttgtcatac tcatcattta tcaacgcttc tttttttgct
     1141 cttgaccatt tttttattct tttctctgta tctattgcaa aattaatatc tgtgaattca
     1201 caataataaa ccaattcaac aggtcatttg tttgctgtat aacaatcagg ataaaaacct
     1261 gtttcgtgct gaaaaagtcg ttgagttaaa ttactcgtca ctcctgtata ataagaatga
     1321 tcagaacatt ttaaaatata cacataagat tgtttcattt atagataaat tttaaaaggg
     1381 ttctcgatac aatttttaaa gcactacttt ttttatttaa tgaacattta tcaagcttta
     1441 aaaatcactc gaactgacga gtaaaataaa cgcttttatt gtcaaagact acaattcgat
     1501 actttctcca atttcaagga gcattaaatc ttttccttta tcaaaaaaat tgcgaatagc
     1561 ttcttcgtga ttgatttcga tgtagccaaa agtatcgtaa tgagcgccta aaatcttatc
     1621 gcattctata aaatcggaag cgattatagc atcctcgaca tccattgtga aattgctacc
     1681 aattgggagt attgccaaat cgagtttggt tcgcatcgga atgagtttca tatccattgt
     1741 taaggatgta tcgccagcaa tgtatatgtt tttatgctcg ccttcaatca caaaaccacc
     1801 aggatttccg ccattacttc cgtcgggaaa agaactagaa tgaattgcgg ttacatattt
     1861 tacttttcca aagtcaaaat tccagcttcc tccgtgattc atggggtgtg attttaaccc
     1921 tttttcggca taatgtgtgg caatttcgta attagataca attactgcat tggttcttcc
     1981 agcaatatat tcaacatcaa gaacgtgatc accgtgagcg tgggtcaaca aaatataatc
     2041 ggcagtaagc tcgttgatat tgatgtgtga agcattggga ttggcagaaa tataagggtc
     2101 aaccaaaatg tgtttcccac caacttgaat tcctaatgaa ccgtgaccgt aaaatgttat
     2161 tttcattttt gatattttta agtgatatat tcaaactgtt agactcttaa acctttaaac
     2221 ccttaaccaa agatttatct tcctcccaaa aagagattta cgattaggtc ttgaattagc
     2281 gaaatcatac aaattgctaa aagcatagaa agtgcaaaag tgcttaaggc taattttttc
     2341 aattcagggt ctaatgctct aggctcttga tttttatata cggtaatcaa atgtttagtc
     2401 aaagggatgt aggccaataa aaataagtat tgatccaagt taaaatcatt tagtattgca
     2461 aaaaccaaaa ccaaaaccat tgcggaaacg attagaaaat aatggtattt tttggctttt
     2521 tctccgccaa tttttacaac aatggtattt ttatttgact ttcggtcaga agcttcatct
     2581 ctcatattat tgagattcaa aacccctaca ctcaaaaaac cgatggcggt ggcaggcaaa
     2641 attaaaggcc attcgacttc ttttgtatat aaaaaattga ctccaatagt actcaccaat
     2701 ccaaagaata taaaaacaaa taaatcgcca aatcctctgt atccataagc agtattgcca
     2761 acagtataac gaattgccga agcgattgcc aagattccca agaccaaata aaacaaagaa
     2821 taaccaatat ttgtatcctt gaaagcaaaa taaatcaaca atattgccga aattagtgtc
     2881 agaaacgaag taataaccaa ggcacgtttc atggcttgtg gagaaatcaa accgctttgt
     2941 atagcccgtt ttggtccaat tcgatcttcg ttatcagttc ctttcattcc atcgccataa
     3001 tcattagcaa aattggataa tatttgcaaa ccaagcgtag tgagaatggc aaaaccaaaa
     3061 atggtccaac tagccacttc ggttggagtg agttcaaaat ctgtagggtg ggctaaagcg
     3121 tacatacttc ccactactat tccggaaacg gataggggca aggttcttaa tcgtgcggct
     3181 tgaatccaat gcttcatttt ttgtgtatta agtatagtga attaagtatt aagattgttt
     3241 aaaggtttaa aatggtttaa agttatttga aaatttaaga ttcctatgca actttaaact
     3301 tttaaacctt aaactattaa acatttttat ggtaaccatt ttttttcaaa attcggtttt
     3361 ctcttttcga ggaaagcatt tctaccttct ttagcttctt cggtcatata ggccaatcga
     3421 gtagcttcgc ctgcaaaaac ttgttgtcct accattccat catcagtaag attcatagcg
     3481 aattttagca ttttgataga ggttggtgat ttttccatta tttcttgtgc ccattgataa
     3541 gcggtatctt cgagttcatc gtgtggaatt acagcattaa ccattcccat ttccatagct
     3601 tcttgagcgg aataatttcg cccaaggaag aaaatttcgc gtgctttttt ctgtccaacc
     3661 attttagcca aataagccga accataacct gcgtcaaaac tggttacatc ggcatcggtt
     3721 tgcttgaaaa tagcgtgttc tttactcgct aaagtcaaat cgcaaactac gtgtaaactg
     3781 tgtccaccac caacagccca acctggaact acggcaatca ctacttttgg cataaagcga
     3841 attaatcgtt gcacttctag aatatttaaa cgatgctgac catcatcacc cacataacct
     3901 tgatgtccac gagcattttg gtcaccaccc gaacaaaagg aatacactcc atctttagtt
     3961 gaagggcctt ctgctgaaag caaaaccact ccaatagagg tatcttcttg agcatcataa
     4021 aaagcttgat ataattctga tgtagtttta ggacgaaacg cattgcgaac attgggtctg
     4081 ttgaacgcta ttcgagccac accattgcat tttttataag tgatatcttc gtattctttg
     4141 acggtaatcc agtccatttt attcggttta aaatataaat gtaaaaatag ctcatttttt
     4201 agatttatct aatacttctg aaattaaaaa tgcagactaa ttcaaggcat tcgctttcaa
     4261 aagttttcgc cacgaattac actaataatc acgaattaaa attgtttttt attgtacgaa
     4321 ttattagaga taaatgaaat tagtttaatt caaacccgtt gggtgtaatt aatttttaat
     4381 actaattttc gtcagttcga gtgcttttcg atagaaaagt gtatcgagaa caaaaaaatt
     4441 aggattaaaa atggttctcg atacattatt tgttccgttt tactccacaa aaaaaactcg
     4501 aactgacgat tttaataatt acacccaacg ggttaattca atttttaaat tacactaatt
     4561 tctaaaagtg agagaattag tgtgattaat tttaacaata attaaatctg cctattgtta
     4621 gatttagatt tgtggtaatt tgtgtaattt gtggtaaaaa aaagtatgag tttgccctgc
     4681 cagactaatt cttaaaatta ttaggaaaaa tattttaact atatttgcag ccgttgtaaa
     4741 taaccgagag caaaatcggt tgcatctatt tatttgaata tccaaatagt ataaattaaa
     4801 aagtgcttta tagtcatact aaaaaagtgt tttttatttt aaatttttct ttctatgact
     4861 cctaaaaagg cttttttttc tatcgcacta ttatttttta tttacacttc tacgttggca
     4921 caacagttag taagcaatga tttaactgta gaagttactc cagaatttgt tgatagcacc
     4981 gctgtgaatc tagacaaaac tacgctagta cttaatgaca gccttacggc aataaacgat
     5041 tcggttgcac cgattgtaca aaatgttccc gcagcttatc caccaccata tcgagattat
     5101 cgaagattgg gctatgatac agctttattt gctggagctg ctgttgtagg ttttggagta
     5161 ctttgggtaa tgccagaaag tgttaccaat tgggataaag aggagatgag agacaagggt
     5221 gttttttgga aatggaaaga aaatgtaaaa gctggccccg tttgggacaa agatgattgg
     5281 gtattaaact ggattacaca tccttattgt ggaggtattt attatatgac agcccgaagc
     5341 agtggtttta cagcattaga atcttttggt tattcggcaa taatgtccac attcttttgg
     5401 gagtatggta ttgaagcttt tgcagaagtt ccctcgattc aagatttaat cataacacct
     5461 gttgttggtt ccgtggttgg agaagggttc ttttatgcca aaaaatctat tgttcgacac
     5521 gataagaaag tcttgaaatc aagattttta ggaataacaa ctttattttt aatggatcct
     5581 ttcaacacta ttttggacgg atttgggtat aaagaaaaag tgaaaactca gatgaatatt
     5641 gctccagttg gggtcaatca attttccaaa acacctgttt ggggtgttag tttctcagcc
     5701 atattttaaa tcaaatttta ctggagttat tctaaaaaaa caccgcaaat tctcaaatta
     5761 tttgaccctt ttggataaaa gaaatttgag aatttgcggt ttatttatgc attttttaaa
     5821 gagctttttc tttcactaga taaattccgt cttccttaat ttcaattagt ttttctttat
     5881 ataacgctcc gatggctttc ttgaaggttt tcttgctcat tttcaagacc gttttgatgt
     5941 cttcgggatg ggagttatct gttaatcgta agaaaccacg actcgcacga agttcatcta
     6001 gaattttctc ggcattaggt tcaatatttt gatacccttg aatttgtagc gaaacatcaa
     6061 ttttatgatc gggacgaatg gttttgatgt atcctctcaa tctatctcca gttcgaatcg
     6121 aatcgtcata cacttcatct ttgtacaaca atcctttatg acgctcgttg atgattacat
     6181 tgatacctaa ttcggtaata tgtgaaacaa ttaaatcaac ctcttcgcct ttttcgactg
     6241 ttaaatggtc atttttcaag aactggttgg ttttactgga cgccactaaa cgtttggttt
     6301 tttcgtccat ataaagatac accaaatagc gttttccttt ttccattggt cgagcttgct
     6361 ctttgaaagg aaccagaata tctttttcca ttccccaatc cataaaagcc cccacttgat
     6421 tgacataatt cacacgcaaa agggcaaatt cattcaacaa aatataaggt tctaatgtgg
     6481 tagcaactgg gcgttcttcg tggtctaaat aaacaaaaac aataatttct tcgcctattt
     6541 cccattcgtt gggaacgtat ttattgggta aaagtatgtc gtgaattcct tcgggatctt
     6601 tttcgggatt tcccaaaaac aaacccactt tggtatcacg taatatggta agggtgttgt
     6661 attttcctat ttcaatcatt ttattctaat tttctaaagc gcaaaggtac gaagattgaa
     6721 aggatttatt ttgaagctaa tgtatagaag ttttattcta actcctttag aattttctct
     6781 atttgaattt taaacgttgg gatggtttca aagatagttt cccaaacaaa actgttgtca
     6841 accgaaaaat attcgtgaac aataaaattg cgcattgatt ttgcaccacg ccaatctacg
     6901 tctggaaatt taagcttaat ctcttcatct atttgcgaaa tggcttcacc tataactatg
     6961 aaatttttaa gaacagcatc ttgtttaagc caattattca agaattcttc tttggttgta
     7021 gttgtcgcca ctaactccaa tttttcaatg gcttcaagaa tatgaaggaa gtaaatctta
     7081 tatttttcag ttttcataaa aataccttac ttcatttagt actttttcct tgataaactt
     7141 attcaagccg ttttctgaaa ttaaatctac ttttttgtgt agtttctctt ctaattcaaa
     7201 atgaatttta gataaagtaa aaagtcca
//
LOCUS       DAOSNN010000127         7215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10376_length_7215_cov_4.838966, whole genome shotgun sequence.
ACCESSION   DAOSNN010000127 DAOSNN010000000
VERSION     DAOSNN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10376_length_7215_cov_4.838966"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLQ70_07465"
     CDS             complement(<1..181)
                     /locus_tag="PLQ70_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQE34490.1"
                     /translation="MDYRLFQSTPAWELAKAVKSGDIKKIKEEVIQKKVPVDYLESRF
                     GHTLLMMAIYNNEYEL"
     gene            complement(349..3255)
                     /gene="infB"
                     /locus_tag="PLQ70_07470"
     CDS             complement(349..3255)
                     /gene="infB"
                     /locus_tag="PLQ70_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQE34491.1"
                     /translation="MSEEKVIRINKVLRELNISLERAVECLKDKGVSVESNPNTKISE
                     AEFNILQNQFAGDKGNKEASKEVGEEKRKEKEALRIEREKEVEDKRKQDEERQKSQEV
                     IRAKGTISGPVHVGKIDLNPKKVIPTPTPEPVVEKTPPPVPVAEIPQAKPILSEPIQK
                     ETPLKVTLLKEPVQEKVIPEAIVNKPVAEAKEIKTEAVAPKAATPQVNEVPAEETITT
                     KYQKLSGATLTGQTIDLSQFNKPKKKKEEPKITPNKPGTPGAPGSAANNANKNKRKRI
                     APKPGAPRPPATPGVPGAPNPNKITPNAGGGGGFNVNRGARPGFVKGARPAIVAKVEP
                     TEEEVKNQIRETLEKLQGKGGKSKAAKYRRDKRDTHRQKSDDEQRALDEGSKTIKVTE
                     FVTVGEIAIMMDVPITKVIGTCMSLGIMVTMNQRLDAETLTIVADEFGYEVEFITVDI
                     EEAIQVVEDKEEDLVFRAPIVTVMGHVDHGKTSLLDYIRKENVIAGESGGITQHIGAY
                     GVTLEDGQKIAFLDTPGHEAFTAMRARGAQVTDIAIIVVAADDDIMPQTKEAISHAQA
                     AGVPIIFAINKIDKPNANVEKIKEKLAGMNLLVEDWGGKIQSHDISAKFGMGVKELLE
                     KVLLEAELLDLKSNPNKAAQGTVVEAYLDKGKGYVSTILVQHGTLRVGDYMLAGKHHG
                     KIKAMHDERGNIVKEAGPSTPVSVLGLDGAATAGDKFNVFEDEKEAKQIAAKRSQLMR
                     EQSVRTQRHITLDEIGRRIALGQFKELNIILKGDVDGSVEALSDSFSKLSTDEIQINI
                     IHKGVGAITETDVMLASASDAIIIGFNVRPAGNARQLADKEEIDIRYYSIIYAAIDDL
                     KDAMEGMLAPEMKEEVLGTAEIRELFKISKVGTIAGCMVVDGKILRNAKIRIIREGVV
                     VHTGELIALKRFKDDVKEVAKGYDCGIQIKGYNDIEERDLIEAFHEVAIKKKLK"
     gene            complement(3352..4605)
                     /gene="nusA"
                     /locus_tag="PLQ70_07475"
     CDS             complement(3352..4605)
                     /gene="nusA"
                     /locus_tag="PLQ70_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQE34492.1"
                     /translation="MENLALIDSFSEFKDDKLIDRVTLMAILEDVFRNALKKKFGSDD
                     NFDIIINPDKGDMEIWQRRVIVADDDLDLEHLEITLSDARKIEPDFEIGEEVSEEVKL
                     IDLGRRAILALRQNLISKIHEHDNTNLYKQFKDLIGEIYTAEVHHVRPRVVILIDDEG
                     NEIVLPKEKQIPSDFFRKGDNVRGIIESVELKGNKPQIIMSRTSEKFLEKLFEQEIPE
                     VFDGLIIVKNVVRIPGEKAKVAVDSYDDRIDPVGACVGMKGSRIHGIVRELGNENIDV
                     INYTSNIQLYITRALSPAKVSSIKINEDTKRAEVFLKLEEVSKAIGRGGHNIKLAGQL
                     TGYELDVIREGDVAGTVADEDDVELTEFSDEIEEWVIEEFAKIGLDTAKSILKQDVDD
                     LVRRTDLEEETILDVMKILKEEFDS"
     gene            complement(4619..5083)
                     /gene="rimP"
                     /locus_tag="PLQ70_07480"
     CDS             complement(4619..5083)
                     /gene="rimP"
                     /locus_tag="PLQ70_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044047587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HQE34493.1"
                     /translation="MTFKDKVNGLVIEALLEKPSVFLIDLTITDSFKIIVNLDGDNGV
                     ALQDCIDISRAIENNLDREEQDFSLEVASVGVGSPLKMVRQYKKNVGRTLIVKLATET
                     IEAELVEANDNFIILSWKAREAKKLGKGKETVQKRQEIPYVEIKEAIVTVTF"
     gene            5263..6087
                     /locus_tag="PLQ70_07485"
     CDS             5263..6087
                     /locus_tag="PLQ70_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQE34494.1"
                     /translation="MKRILVPVDFSEYSIEALKVAAQIAQKNNCEIILLHLLELPHQS
                     TDPFGNSNSIPEIIFFKNKAIEKLETLIDSPFLQGIEVLESIEFKKVDEGVVDASIKN
                     DVDLIVMGSHGTSGFNELLVGSNTEKIVRYSKIPVLVIKKGVKEFKGNNFVFASDFLK
                     ETRKPFRKMLEFAKLFDSKLFLVTICTPASFKTTSTTEKNMNDFVAHFDIENYSTHIY
                     NDTNIENGIINFAKSVNADIIGICTHGRTGLAHFYNGSISEDLVNHVSKPVITFKI"
     gene            complement(6154..6224)
                     /locus_tag="PLQ70_07490"
     tRNA            complement(6154..6224)
                     /locus_tag="PLQ70_07490"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6190..6192),aa:Gln,seq:ttg)
     gene            6456..>7215
                     /locus_tag="PLQ70_07495"
     CDS             6456..>7215
                     /locus_tag="PLQ70_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HQE34495.1"
                     /translation="MTTFNFNKWNTITGWCAFAIALITYTLTVEPTMSFWDCGEYIAT
                     SAKLQVGHPPGAPLFQMIGAFFAMFAMDDKHIALMVNMMSVFSSAFTILFMCWSSSMI
                     LKKIISQYSEINKNNSIAILGSSFVGALAFTFSDSFWASAVEAEVYAMASLFIALLFW
                     LGLRWEQDMDSPKGNKWLLVISIVVGLTFGVHFMALLTIPAIGFLYYFKHYKEVTIKN
                     FIIANIVVVAVLLFIFKLLLPLTMAFFGKTEVFMV"
BASE COUNT         2084 a         1412 c         1067 g         2652 t
ORIGIN      
        1 atagttcgta ctcgttattg taaatagcca tcatcagtaa ggtatgacca aaccttgatt
       61 ccaaataatc tacgggtact tttttttgga tgacctcttc ttttattttc ttgatgtcac
      121 cactcttaac tgctttggca agctcccaag ctggagtact ttggaacaat cgataatcca
      181 tacctaacag tttactttta tcaacttgtg tttcttgatt gtaacaactg gtaagacaaa
      241 ataaaagaca aatagaaatt aattttttca tactttaaat tattatcaac caaagctaaa
      301 caaaaaaaat cccgattgct cgggatttct tatgaatata tcgaaatttt atttcaattt
      361 ctttttgata gctacttcgt ggaaggcttc gattaagtct ctttcttcga tgtcgttgta
      421 tccttttatt tgaataccac aatcataccc tttagcaact tctttgacat cgtctttgaa
      481 acgtttcaag gcaatcaatt caccagtgtg aaccacaaca ccttcgcgaa taattctgat
      541 tttggcattt cttagaattt ttccatccac aaccatacat cctgcaatgg tacctacttt
      601 agagatttta aacaactctc taatttcggc agtaccaagc acttcttctt tcatttctgg
      661 tgccaacatt ccttccatcg catctttcaa gtcgtcgatt gcggcgtaaa tgatagaata
      721 gtaacggata tcaatttctt ctttgtcggc taattgtctt gcatttccag caggacgaac
      781 attaaatcca atgatgatcg catccgaagc agaagccaac ataacatcgg tttcagtaat
      841 cgctccaact cctttatgaa taatattaat ttggatttcg tcagtcgaaa gtttagagaa
      901 cgaatcagac aaggcttcaa cagaaccatc tacgtcacct ttaagaatga tgttcaattc
      961 tttaaattga cccaaagcga ttcgacgtcc aatttcatct aaagtaatgt gtcgttgtgt
     1021 acgaacagat tgttcacgca tcaattgaga acgtttggcc gcaatttgtt tggcttcttt
     1081 ttcgtcttca aaaacattga acttatcacc agcagttgca gcaccatcta aacctaatac
     1141 agatacaggt gtagaaggtc cagcttcttt aacaatattt ccacgttcat catgcatcgc
     1201 tttaatctta ccgtgatgtt ttcctgccaa catgtaatct ccaactctta gagttccgtg
     1261 ttgtactaat atggtagaaa catatccttt ccctttatct aaataagctt caacaacagt
     1321 tccttgtgcc gctttattag gattagattt caaatctaaa agttctgctt ctaataatac
     1381 tttttctaat aattctttaa cacccatccc gaatttagct gaaatatcgt gtgattgaat
     1441 ttttcctccc caatcttcaa ccaataaatt catacctgct aatttttctt ttattttttc
     1501 aacattagca tttggtttat caatcttatt gatagcaaat ataattggaa caccagcagc
     1561 ttgagcgtga gaaatggcct ctttggtttg tggcataata tcatcatcgg cagctactac
     1621 aataatagcg atatcggtaa cttgagcacc acgcgcacgc attgcggtaa acgcttcgtg
     1681 acctggcgta tcaaggaaag caattttttg accgtcttcc aaagttactc cgtaagcacc
     1741 aatgtgctgt gtaattcctc ctgactcacc tgcgataaca ttttccttac gaatataatc
     1801 caataaagat gttttaccgt gatctacgtg acccattaca gtaacgatag gagctctgaa
     1861 aactaaatct tcttctttgt cttcaacaac ttgaatcgct tcttcaatat caacagtgat
     1921 aaattctact tcatagccaa attcatccgc aacaatggtt agcgtttctg catcaagacg
     1981 ctgattcatg gtaaccataa ttccaagtga catacaagtt ccaatcactt tagtaattgg
     2041 cacatccatc ataatagcga tttcaccaac agttacaaac tctgtaactt taatggtttt
     2101 gctaccttca tctaatgctc tttgttcatc atcggacttt tgacggtgtg tatctctttt
     2161 atctcttcta tattttgctg ctttcgattt accacctttt ccttgtagtt tctctaaagt
     2221 ctctctaatt tgatttttaa cttcttcttc ggtaggctca actttagcaa caatagcagg
     2281 tctcgctcct tttacgaagc caggtcttgc acctctgtta acattaaatc ctccaccacc
     2341 tccagcattt ggagtgattt tatttggatt tggtgctccg ggaactccag gcgttgctgg
     2401 tggtcttggc gcacctggtt ttggagcaat cctttttctt ttatttttgt tggcattatt
     2461 tgccgcactt ccaggagctc caggagtccc gggtttgtta ggcgtgattt ttggctcttc
     2521 ttttttcttt ttaggtttat taaattgaga taaatcaatg gtttgccccg ttaaagtagc
     2581 accagataat ttttggtatt ttgttgtgat tgtttcttca gcaggcactt cgtttacttg
     2641 aggagttgct gctttaggag ctacagcttc cgttttaatc tctttagctt cagcaacagg
     2701 cttattcact attgcttcag gaatcacttt ttcttgaact ggttctttta gaagagtcac
     2761 ttttaacgga gtctcttttt gaattggttc gcttagaata ggttttgctt gaggaatttc
     2821 ggctactgga actggtggtg gagttttttc aacaactggt tcaggtgttg gagttggaat
     2881 tacttttttt ggattaagat caatttttcc aacgtgaact ggcccagaga tagtaccctt
     2941 agctctgatg acttcttgac ttttttgacg ctcttcatcc tgtttgcgtt tgtcttcaac
     3001 ttctttttct cgttcaatac gcaatgcttc tttctctttc cttttctctt cccctacttc
     3061 tttggaagcc tctttattcc ccttgtcgcc agcaaattga ttttgcagga tattgaattc
     3121 tgcttcagaa atttttgtgt taggatttga ttcaacagaa acccccttgt ctttaagaca
     3181 ttccacagct ctttctaaag aaatatttaa ttccctcaaa actttgttta ttctaataac
     3241 tttctcttca gacataaaac ctttttaata ttaccttctt tattgttcct caaactaact
     3301 ctctgctaga gaagaaaata aggtttgaaa tttatttttt taaagaattg actagctgtc
     3361 aaattcttct tttagtattt tcattacatc tagaattgtt tcctcttcta ggtcagttct
     3421 tctaaccaaa tcatctacat cttgtttcaa gatacttttt gctgtatcta aaccaatttt
     3481 tgcaaattct tcgataaccc attcttcaat ttcatctgaa aactcagtta attcaacatc
     3541 gtcttcatct gcaaccgtac ccgcaacatc accttcacga attacgtcaa gctcataacc
     3601 tgttaattga cctgccaatt taatattgtg tccacctcta ccaattgctt tagaaacttc
     3661 ttctaatttc aaaaacactt ccgctctttt tgtgtcttca tttattttga tagaagaaac
     3721 ttttgcagga ctcaatgctc tagtaatata caattgaata ttgcttgtat agttgataac
     3781 atcaatgttt tcgtttccta attcacgaac aattccgtga atacgggatc ctttcattcc
     3841 cacacaagca ccaactggat caattctatc gtcatacgaa tctacagcta cttttgcttt
     3901 ttcgcctgga attctcacta cattttttac aataatcaat ccgtcaaaaa cttctggaat
     3961 ttcttgttca aataattttt ctaagaactt ttctgaagtt ctagacataa ttatctgagg
     4021 tttatttcct tttaattcaa cgctttcaat gattccacga acgttatccc ctttacggaa
     4081 aaagtcagat ggaatttgtt tttcttttgg caaaacaatt tcatttcctt cgtcgtcaat
     4141 caaaattaca actcttggac gaacgtgatg tacttcggca gtataaattt cgcctattaa
     4201 atctttaaat tgtttataaa gattagtatt atcgtgctca tgaattttag aaatcaggtt
     4261 ttgacgcaat gctaaaatag ctcttcttcc taaatctatt agtttcactt cttctgaaac
     4321 ttcttctcct atttcaaaat caggctcaat ttttctagcg tcagacaatg taatttctaa
     4381 atgctctaaa tctaaatcat catctgcaac gataactctt ctttgccaga tttccatatc
     4441 tcctttatct ggattgataa tgatgtcgaa attatcatct gatccaaatt ttttcttcaa
     4501 tgcatttcta aacacatctt ctaatatcgc cataagcgtt acacgatcaa taagtttatc
     4561 atctttaaac tctgaaaatg aatcgattaa tgctaaattt tccatgccaa ctatttaatt
     4621 aaaatgttac tgtaacaatt gcttctttta tttctacgta cggtatttct tgtctttttt
     4681 gaaccgtttc ttttcctttt cctagttttt tggcttctct agctttccat gacaaaatta
     4741 taaaattatc attagcttca actaattctg cttcaattgt ttcggttgct aattttacta
     4801 ttaatgttct acccacattt ttcttgtatt ggcgtaccat tttcaagggt gaaccaacac
     4861 ctaccgaggc tacttctaaa gaaaaatctt gctcttctcg gtccaaatta ttttcaattg
     4921 cacgacttat gtcaatacaa tcctgcaaag ctactccatt atcgccatct aaattcacga
     4981 taattttaaa actatccgtt attgtcaaat caatcagaaa aacagaaggt ttctccaaca
     5041 atgcctcaat tactaagccg tttactttat ctttaaatgt catattttat aaaaagaggc
     5101 acgctttgcg tgcctcatta tctagttttt taataaataa ctgtgcaaat atagtgtttt
     5161 ttttataaat caaaaatgat tgtttcatgt taaaaatatt ttgtaaattt aatcaaccaa
     5221 atttcatgtc ctgtttgtcc aataatctaa atacttttta ttatgaaacg gattttagta
     5281 cctgtcgatt tctcggaata ttcaattgaa gctctaaaag tagccgctca aattgcccaa
     5341 aaaaacaatt gcgaaattat acttttacac ctattagagt tacctcatca atcaaccgac
     5401 ccttttggca atagcaatag tattcctgaa attatatttt ttaaaaacaa agctattgaa
     5461 aagcttgaaa cactaataga ctctcccttt ttgcaaggaa ttgaagtact tgaatctatc
     5521 gaattcaaaa aggttgatga aggggttgtt gatgcaagta taaaaaacga tgtcgattta
     5581 attgtaatgg gatctcacgg aacatctgga tttaatgaat tattagtagg ttctaatacc
     5641 gaaaaaatcg ttcgttactc aaaaattccc gttttagtaa tcaaaaaagg agtgaaagaa
     5701 tttaagggga ataattttgt tttcgcctca gattttttaa aagaaactcg aaaaccgttc
     5761 agaaaaatgc ttgaatttgc aaaacttttc gattctaaac tattcctagt aactatttgt
     5821 actccagcca gttttaaaac cacgagcaca accgaaaaaa acatgaatga ttttgtcgct
     5881 catttcgata tcgaaaatta ctccactcac atctataacg acacaaatat cgaaaatgga
     5941 attattaatt ttgcaaaaag tgtaaatgcc gatataatag gaatctgtac acatggtcga
     6001 acaggattag ctcattttta caacggaagc attagtgaag acctagttaa tcatgtgtct
     6061 aaacctgtta taactttcaa aatttaaata ccaaagttaa ctcgttgaca attaagcata
     6121 aaaaaacctc tcaataattt gagaggtttt ttgttggtct acaaggactc gaaccttgaa
     6181 agactgcacc aaaaacagtt gtgttaccat tacaccatag accaattcca ttgctgtaaa
     6241 gcgagtgcaa atttaataca aaatttaatt tctacaaatt tttgagcaat tttattttaa
     6301 ttattttttt gttgatttta aaccttatac tatagattgc caaaaagtaa tttgttgaat
     6361 ctgttttttg ggaatttttt gttaatgcac gtatgtttaa ataaaaaaac attttctttg
     6421 tatctgaaaa ataattaatt ttttgcttac aaaatatgac aacatttaat ttcaacaaat
     6481 ggaatacaat tacgggttgg tgcgcatttg caattgcatt aatcacctat acacttactg
     6541 ttgaaccaac aatgagtttt tgggattgtg gcgaatacat tgctacgtct gcaaaacttc
     6601 aagtgggaca tcctcctgga gctcctttgt ttcaaatgat tggtgcgttt tttgccatgt
     6661 ttgcgatgga tgacaaacat attgctttaa tggtaaatat gatgtctgtg ttttctagtg
     6721 ctttcacgat tttgtttatg tgctggtctt catcgatgat tttgaaaaaa ataatttcgc
     6781 aatattcaga aattaataaa aacaattcta ttgccattct cggcagttcc tttgttggtg
     6841 cattggcctt tactttttcc gatagttttt gggcaagtgc agtcgaagct gaagtttatg
     6901 caatggcttc cctatttatt gcgttacttt tttggctagg attgcgttgg gaacaagata
     6961 tggattcccc taaaggcaac aaatggctgt tggttatttc gatagttgtt ggacttacat
     7021 ttggagttca ttttatggca ttattaacca ttccggctat tggttttctt tattatttta
     7081 aacattataa agaagttaca atcaaaaatt ttatcattgc caatattgtt gttgtcgctg
     7141 ttttattatt tattttcaaa ttacttttgc ctctaacgat ggctttcttt ggtaaaaccg
     7201 aagtttttat ggtaa
//
LOCUS       DAOSNN010000128         7201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10396_length_7201_cov_5.069130, whole genome shotgun sequence.
ACCESSION   DAOSNN010000128 DAOSNN010000000
VERSION     DAOSNN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10396_length_7201_cov_5.069130"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLQ70_07500"
     CDS             complement(<1..398)
                     /locus_tag="PLQ70_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34496.1"
                     /translation="MEYFTLARVIHVIAVILWIGGVSMVTTVLIPAIKKMKSKEERIA
                     TFEMIEGKFATQAKITTLLTGLSGLYMLYVLDGWSRYLDYRFWWIHAMTLVWFIFTLI
                     LYVLEPLVLHKIFKKLSAIMSFTKSFPSLKA"
     gene            complement(602..2794)
                     /locus_tag="PLQ70_07505"
     CDS             complement(602..2794)
                     /locus_tag="PLQ70_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HQE34497.1"
                     /translation="MTLSFWRYSHLALALFSAAFLILASVTGVILAVDAVQEKTLGYR
                     VENFNAITLGETLPVLRKTYPEITELSVDYNQFVTLQGIDQEDNDVNAYIDPRTGKIL
                     GKPIKKSEFINWTTALHRSLFLKEKGRFIVGSISFILALISISGFVLILKRQKSIRSL
                     FSKIIKENFAQYYHIFLGRLSLIPILVLALSGTYLTLEKFNFFLEKTDSEEKTEVVKT
                     TPISGKNTSSIFKSTLLADVQKIEFPFSDDPEDFYIITLNDREIEVNQITGAVVNEKL
                     FPKTTQLSNLSLDLHTGRASILWAIILGIASINILFFIYSGFAMTLKRRASRIKNKYK
                     AEESKFILLVGSENGSSLRFANAVHKQLLAHGEKVFIAELNNYAVYPKAEQLIIFTST
                     HGLGDPPSNATKFVSLLNQTNQQQKIKVSVVGFGSKSYPDFCGFAREIDAELAKQNWA
                     ERLLELQTVNDKSAEEFVDWTTLWSAQTGIPLSATPSLYNEVPKGLKKLVVLSKTAVS
                     ETEHSFLVTLRAGLGTQFTSGDLLAIYPANDNRERLYSIGKHDDNIQLVVKLHPHGLG
                     SGYLNILEIGSTIKARIIQNPAFHFPKKASKVALISNGTGIAPFLGMIEQNKQKTEIH
                     LYCGFRTETETVSNHKKFANEMMEKQQLQSFHLALSREADHHYVMDLIQRDVVFFLDL
                     LQKGGIIMICGSLAMQKDVETILDELLAQTNSSISDYKTKGQILTDCY"
     gene            complement(2912..3394)
                     /locus_tag="PLQ70_07510"
     CDS             complement(2912..3394)
                     /locus_tag="PLQ70_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009087353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HQE34498.1"
                     /translation="MKSIFKIAFTTALICLLSFQATAQTSKYKCMLQMSNYMGEGAYI
                     VVSLISPKGEYEKTLYVMGEDKKWYKTVKEWHKFQSKKKEDISAKTGASVTGGDRSIT
                     TIEIDDAKINKGYKLRFETAVEDNKYYVSDAEIPLTTEGLAAKTDGKGYIRYVRFNKI
                     "
     gene            complement(3467..4984)
                     /locus_tag="PLQ70_07515"
     CDS             complement(3467..4984)
                     /locus_tag="PLQ70_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQE34499.1"
                     /translation="MKKNLFISFALVASLIVSAQQKNTLLDQSFWKNAPDVTAVQAEI
                     AKGNNPSASTANFFDAVVYAINNDAPTATIKFLLDQPGNPVNKTTHDNRIYLHWAASK
                     GNLEIVEYLIAKGSDVNLEDSHSAFPITAAASSGQSNTAIYEALFKAGVDPKKKYANG
                     ANVLLTAIPFDKNLVLTNYFITKGMSLAAVDDDGSNAFDYAARTGNQTLLKTLLEKGV
                     KPTDNALLIAAQGSRRDANTLETYKYLVEDLKIKPTATNKDGQNVFHSLVTKSNQTEI
                     INYFLTKGVDINKADNDGNTPLMNAVSGRETNESLAQLLPLIKNINIQNKKGESALTK
                     AIQSGTAETVALLLEKGADIKVLDKDGNNLAFYLVDSYRPQMMGMGRPTEGNAPKQDP
                     FAAKTKLLQDKGLNLAAPQKDGSTLYHFAVVKNDLNLLKKLADLNIDINAKNKDDLTA
                     LHKAAMIAKDDAILKYLLSIGAKKEIKTDFDESAYALAKENETLTKKKTDLEFLK"
     gene            complement(5323..5796)
                     /locus_tag="PLQ70_07520"
     CDS             complement(5323..5796)
                     /locus_tag="PLQ70_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HQE34500.1"
                     /translation="MKKVILSLLIILTLASCQKSRVVSKIVGTDWLLGKWENKSDEGN
                     LLETWTKVNDSVFIGESYFIKEKDTLHSEKIQLQQKGENLLYVSTIKGQNNDKAITFV
                     HKPEIEKQLVFENPKNDYPKKIAYKRIAKDRLIIEISGIQQGKSSSDRYSMKKTE"
     gene            complement(6123..>7201)
                     /locus_tag="PLQ70_07525"
     CDS             complement(6123..>7201)
                     /locus_tag="PLQ70_07525"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HQE34501.1"
                     /translation="VLFKTTGKEILKKGFRVVFEDPNAKEKEADILPSFVVGEKGPHE
                     PSFLEKQTKPPNQFTEATLLRAMETAGKQVEDEDLRELMKENGIGRPSTRANIIETLF
                     RRQYIVRNKKQVLPTPTGIQLIDTIQNELVKSAELTGSWEKQLKDIEKGTFTAGAFIK
                     NMKQMVETLVYEVRSETRRANISHTQSIQKQEAVVEKKKADGILAEVCPKCKKATLIK
                     GKSAYGCGDYKAGCRFVLPYTFADKKISENQYLRLLQKGSTVNLKDFKTDAGLVEGLL
                     RFDENFELKLEEKKTTAKPISNVLECPKCKKGTVLKGNTAYGCTDYKSGCDFKVPFDV
                     VRAKLKDQKPSKELVYSILKESYL"
BASE COUNT         1956 a         1296 c         1419 g         2530 t
ORIGIN      
        1 gctttaagcg aagggaaact tttggtgaaa gacattattg cagataattt tttaaaaatt
       61 ttatgcaaaa ccaaaggctc cagcacatat aaaatcaagg tgaaaatgaa ccataccaac
      121 gtcattgcgt gaatccacca gaatctgtaa tcaagatatc tgctccagcc gtcgagtacg
      181 tagagcatat aaagtccgga caatccggtc agcaaagtcg ttattttggc ttgggtggca
      241 aattttcctt caatcatttc aaaggttgct attcgttctt cctttgattt catttttttg
      301 atggccggaa tcagtacggt tgtcaccata gaaacgccac caatccatag aatgacggca
      361 attacgtgta tgactcgggc gagagtgaag tattccataa tttcggttta aatgtaaata
      421 tttgttaaat ataggatgtt ttttaatcca gtttcggctc cctctccttt aatttgccag
      481 tttgttgtcg ctcgtgtgga gaggatttat tttggtgcat tatcctgcaa ggtctttttt
      541 tgaccttgta ggtattccaa gttaaaccta caaggttttt gaaaccttgc aggaaaaaat
      601 tctaataaca atcggtcaaa atttgtcctt tggttttgta atccgaaata ctcgaattgg
      661 tttgggcaag cagttcatca agaatagttt ccacgtcttt ttgcattgcc aatgaaccgc
      721 aaatcattat aattcctcct ttttgcaaca aatccaggaa aaaaacaaca tcccgttgta
      781 ttaaatccat cacgtaatga tgatcggctt ctcgggacaa ggccaaatga aaactttgga
      841 gttgttgttt ctccatcatt tcgttggcaa attttttatg gttcgaaacg gtttcggttt
      901 cggttcgaaa tccgcaatac aaatgaattt cggttttttg cttgttttgt tcaatcattc
      961 ccagaaaagg agcaatgccc gttccgtttg aaatcagggc gactttcgat gcttttttcg
     1021 gaaaatggaa agcagggttt tggatgattc ttgctttgat ggttgagcct atttccagaa
     1081 tgttcaaata acccgaaccc aatccgtggg gatgcaattt cacgaccagt tgtatgttgt
     1141 catcgtgttt tccgatggag tagaggcgtt cccgattgtc gttggcggga taaatggcca
     1201 gcaaatcacc cgaagtgaat tgggttccca aaccggcacg aagtgtcacc aaaaagctgt
     1261 gttcggtttc ggaaacggca gttttgctta atacgaccag ttttttcaaa cctttcggga
     1321 cttcattgta taaagacggg gtggcagaaa gcggaatccc ggtttgggca ctccaaagtg
     1381 ttgtccaatc cacaaattct tcggccgatt tgtcgttgac ggtttgcagt tccaaaaggc
     1441 gttctgccca attttgtttg gccaattcgg catcgatttc tcgggcaaaa ccacaaaaat
     1501 caggataaga ttttgaccca aaaccaacca ccgaaacctt gattttttgt tgttgatttg
     1561 tttgatttag caacgaaacg aatttagttg cattcgatgg tggatcgccc aatccgtggg
     1621 ttgaggtgaa aataattagc tgttctgctt ttggataaac ggcataatta ttcaattcgg
     1681 caataaaaac tttttccccg tgagccaata attgtttgtg aaccgcattc gcaaatctta
     1741 gcgaactgcc gttttcggaa ccgaccagca aaatgaattt gctttcctcg gctttgtatt
     1801 tgtttttgat gcggctcgct cttcgtttca aggtcattgc aaaacccgag tagataaaaa
     1861 acaggatgtt gatggaggca attcccaaaa ttattgccca taaaatactg gcacgaccgg
     1921 tgtgcaaatc cagactcaaa ttggataatt gggtagtttt cggaaacagt ttttcattca
     1981 cgacagctcc ggtaatttgg ttgacttcga tttctcggtc atttaaggta atgatgtaaa
     2041 aatcttcggg atcgtctgaa aaaggaaatt caattttctg tacatcggcc agaagcgtgc
     2101 ttttgaaaat agatgaagtg ttttttcctg aaattggtgt tgtttttaca acttccgttt
     2161 tttcttcgga atctgttttt tcgagaaaaa aattaaactt ttccaaagtc aaatacgttc
     2221 ccgaaagggc aagtaccagg atgggaatca aggataatct tccgaggaaa atgtggtaat
     2281 attgggcgaa attttctttt ataattttag aaaataaact gcgaatgctt ttttgccttt
     2341 ttagaataag tacaaatccc gaaatagaaa ttaatgctaa aataaaagag atggacccca
     2401 cgataaagcg ccctttttct ttcaaaaata gagagcgatg cagtgctgta gtccagttga
     2461 taaactcact tttcttgatg ggcttgccca gtattttacc cgttcttgga tcgatatagg
     2521 cattgacatc attgtcttct tggtcaatcc cttgcaaggt tacaaattga ttgtaatcta
     2581 cactcaattc ggtgatttcg ggataggttt tgcgcagcac gggcagggtt tctccaaggg
     2641 ttatggcgtt gaagttttcc actcgataac ccagcgtttt ttcctggacg gcatcaacgg
     2701 cgagaatgac accggttacg gatgccagta ttaaaaatgc agcagaaaac aaggccaagg
     2761 ccaagtggga gtaacgccaa aaagaaagtg tcattgagga tgaatttgtt caaattgttt
     2821 taccatataa ggcatttaag agcatataag ttcgttttta tacaattctt aaatgacctt
     2881 aaatgcctta tatggtaaaa gaaatgtttt tttagatttt attgaagcgg acatagcgaa
     2941 tatagccttt gccatcggtt tttgcagcaa gcccttcggt ggtcaacgga atttcggcat
     3001 cggaaacata atatttattg tcttcaacgg cagtttcaaa tcgcaatttg tatcctttgt
     3061 tgattttggc gtcatcaatt tctatggtcg tgatgctgcg gtcaccgccg gtaacggatg
     3121 caccggtttt ggcactgatg tcttctttct ttttggattg aaatttgtgc cattctttaa
     3181 cggttttata ccattttttg tcttctccca tcacgtacaa tgttttttcg tattctcctt
     3241 tggggctaat caaggaaacc acgatataag ctccttcgcc catatagttg gacatttgca
     3301 gcatacattt gtatttgctg gtttgtgccg tggcttggaa agacaataga cagataagtg
     3361 cggtggtaaa ggctatttta aatattgatt tcatttttta attatgagtt atgagttgtg
     3421 ggttatcggc gctaatgact aatcactaac agctattcac taatgactat ttcaaaaatt
     3481 ctaaatcggt tttctttttg gttaatgttt cgttttcttt tgccaaggca taagcacttt
     3541 cgtcaaaatc ggttttgatt tcttttttgg caccaatgga aagcaggtat ttcaagatgg
     3601 cgtcatcttt ggcaatcatc gcggctttgt gcaaagcggt caaatcatcc ttgtttttgg
     3661 cattgatgtc gatatttaaa tcggctaatt ttttcagcaa atttaggtca tttttgacaa
     3721 ctgcaaaatg gtaaagcgtg cttccgtctt tttgcggagc ggccaaattc aagcctttgt
     3781 cttgaagtaa ttttgttttg gcagcaaaag gatcttgttt tggggcattg ccttcagttg
     3841 gccgtcccat tcccatcatt tgcggacgat atgaatcaac tagataaaaa gctaaattgt
     3901 ttccgtcttt gtcaagtact ttaatatcag ctcctttttc taaaagaagc gcaacagttt
     3961 cagccgttcc agattggatg gcttttgtta aagccgattc tccttttttg ttttgtatgt
     4021 tgatgttttt tataagtggc aacaattgtg ctaaactttc attggtttct ctaccagaaa
     4081 cagcattcat caaaggggtg ttgccatcgt tgtcggcttt attgatatca actcctttgg
     4141 tcaagaaata attgataatt tcggtttggt tcgattttgt taccaatgaa tgaaatacat
     4201 tttgtccatc tttgttggtt gcagtaggtt taatttttaa atcctctact aaatatttgt
     4261 aggtttctaa tgtgttggca tccctacgag agccttgtgc cgcaatcagt aaagcattgt
     4321 cagttggttt tactcctttt tctaaaaggg ttttcaataa agtttggttt ccagttctag
     4381 cagcataatc aaaggcgttg ctaccatcat cgtccacagc tgcaagagac attcctttgg
     4441 tgataaaata attggtaagc actaaatttt tatcaaaagg gattgcagtt aataatacat
     4501 tggcaccgtt ggcgtatttt ttctttggat caacaccagc tttgaataag gcttcgtaaa
     4561 tagccgtgtt gctttgtcca gaacttgcag ctgctgtaat tgggaacgcg ctgtggctgt
     4621 cttctaaatt aacatcagat cctttggcaa taagatattc tactatttca agatttcctt
     4681 tactagcagc ccagtgaaga tagattcgat tgtcgtgagt ggtcttgttt actggatttc
     4741 caggttgatc taataggaat tttatagttg cagtgggagc atcattatta atggcataaa
     4801 caacggcatc aaaaaaattt gcagtcgatg cagatggatt gtttcctttt gcaatttcag
     4861 cttgaacggc agttacatct ggagcatttt tccaaaacga ctgatccaaa agcgtgtttt
     4921 tttgttgcgc acttactatt aaactggcga ctaaggcaaa agaaataaaa agattctttt
     4981 tcataaaatg tatattaagg ttcttattgt agtggtattt gtttttattt tttaccaacg
     5041 aaaagttaga ttttgtttaa aagctaatca tttatttaga ataaataaaa ataaagacaa
     5101 aagtaaaaat aaataataga ataccaaatc attatttagt ctttctaaag tggaattttt
     5161 agaatctgtt ttctttagat tttaattgtg ctaaaaggtt taagcggtta tttgattttt
     5221 tgaaactatt gtcttcttct gctttttatt tcgtccccac caaatataaa atcctgcttt
     5281 tatggagcag gattcattgt aaatgtaaaa aggaagtgcg ctttattcgg tttttttcat
     5341 cgaatagcga tcggaactgg attttccttg ttgaattcca gagatttcta tgattaaacg
     5401 atctttggca attcgtttgt atgcaatttt ttttgggtaa tcattttttg gattttcaaa
     5461 aaccaattgc ttttcgattt cgggtttatg aacaaaagta atggctttgt cattgttttg
     5521 tccttttatg gtcgaaacat acaaaaggtt ttctcctttt tgctgcaatt gaattttttc
     5581 ggaatgcaag gtgtcttttt cttttataaa gtagctttcg ccaataaaga cgctgtcgtt
     5641 tactttcgtc caagtttcca atagatttcc ttcatccgat ttgttttccc attttcctag
     5701 caaccaatcg gttcccacta ttttggaaac tactctggat ttttgacaag aagctagggt
     5761 taggattatt agtagtgaaa gaattacttt tttcataaga ggtgtaaaat tgtagtttga
     5821 aaacttttca aaattaggaa aaagatagca attagttcaa gtctaagaca tcagtttaaa
     5881 ataaaaaatt aaatgcaaat ttttagatca gattatattt gtataatact tatttatggt
     5941 cgttttttgg ttcaaatagt tatacttgag tactttttat tcttgctatt tgtcacgctg
     6001 gaagcgtctc gattatgttg ctcacctgtg ttgagacgct ttcagcgtga caaatggagt
     6061 gaaatgttgt aatgataaag caattgcaat tggtcttaat tcaagtaaat tagtcttgat
     6121 agttataaat aactttcttt caaaatacta taaaccaatt ctttgctagg tttttggtct
     6181 ttcaattttg cccgaaccac atcaaaaggg actttgaaat cacagcccga tttatagtcg
     6241 gtgcaaccat aagccgtgtt tcctttaaga accgttcctt ttttacattt tggacattct
     6301 aatacattcg atattggttt tgcggttgtt tttttctctt ctaatttaag ttcgaagttt
     6361 tcgtcaaagc gaagcaaacc ttctaccaat ccagcatcgg ttttgaaatc tttcaggttt
     6421 acggtcgagc ctttttggag caatcgcaag tattgatttt cggatatttt tttgtcggca
     6481 aaagtataag gcaaaacaaa acgacaaccc gctttgtaat caccacaacc gtaagcagat
     6541 tttcctttga tgagtgtggc ttttttgcat ttcggacaaa cttctgccaa aattccgtct
     6601 gctttctttt tctcgacaac ggcttcttgt ttttgaatgc tttgcgtgtg cgaaatattg
     6661 gcacgtcggg tttcgcttcg gacttcatag accaaagttt cgaccatttg tttcatattc
     6721 ttgatgaatg ctccagcggt aaacgttcct ttttcaatat ctttcaactg cttttcccaa
     6781 gaacccgtca attctgccga cttgaccaac tcgttttgaa tggtgtctat caactgaatt
     6841 ccagtgggtg ttggcaaaac ctgttttttg tttcgaacaa tgtattgtct tctaaaaagt
     6901 gtttcaataa tattcgctcg agttgatgga cgaccaatgc cattttcttt catcaattcc
     6961 cgtaaatctt cgtcttcgac ttgttttcct gcggtttcca tagctcgcag taaagtagct
     7021 tcggtaaatt gattgggcgg tttggtttgt ttttccaaaa acgaaggttc gtgtgggcct
     7081 ttttcgccta cgacaaaact cggcaagatg tcggcttctt tttctttggc gtttgggtct
     7141 tcgaaaacta ctcggaaacc ttttttcaag atttcttttc cagtggtttt gaacaatacg
     7201 t
//
LOCUS       DAOSNN010000129         7194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10407_length_7194_cov_5.676565, whole genome shotgun sequence.
ACCESSION   DAOSNN010000129 DAOSNN010000000
VERSION     DAOSNN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10407_length_7194_cov_5.676565"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLQ70_07530"
     CDS             complement(<1..616)
                     /locus_tag="PLQ70_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE34502.1"
                     /translation="MATYNKRGYKPAKEKEVKEVTEDINVAELEKNSTTAEVFSTLDA
                     TASKTEDFVAKNQKIIIGVVGAIALLTVGYLAYQKFVAAPKEDDAANQMFVAQQDFEK
                     ATNGVASDSLYKLALNGSEGKWGFVKIADEYSGTDAGNLANYYAGIAYLNTGKYAEAI
                     EYLGKFKSDDLVISAMATGAIGDAYSQKNQPKEALDYYVKAAESN"
     gene            858..1937
                     /gene="recF"
                     /locus_tag="PLQ70_07535"
     CDS             858..1937
                     /gene="recF"
                     /locus_tag="PLQ70_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022322.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HQE34503.1"
                     /translation="MYLKKISLFNYKNFSEANFEFVGKINCFVGKNGIGKTNVLDAIY
                     HLSYGKSYFNPLAVQNIKHGEDFFVIDGEFEIKERNEQIICSLKKGQKKILKRNGKIY
                     DKFSEHMGFIPLVIISPADTDLIVEGSETRRKFMDSVISQLDSNYLKHLIQYQKVISQ
                     RNALLKYFALNFVFDNDTLSIYNEQLNTFGQYIFEKRKEFIEQFIPIFNSHHQAISGS
                     QETVQLVYESHLFENDLLTLLEENISKDRALHYTSVGIHKDDLSFEIDKYPIKKFGSQ
                     GQQKSFLIALKLAQFEFLKTQSGQKPILLFDDIFDKLDENRVAKIVEMVNSDTFGQLF
                     ISDTHPERTEKIVKSTHQVYKIFNL"
     gene            2022..2723
                     /locus_tag="PLQ70_07540"
     CDS             2022..2723
                     /locus_tag="PLQ70_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQE34504.1"
                     /translation="MKLLSKFCILFLSLSIVSCNGQNAKNIKNLEPKAFSETLKTTTN
                     VQIIDVRTPQEFAAGHLENAQNIDWLGDNFDATIQKLDKTKPVFIYCKTSNRSSQAAE
                     KLEKLGFENIYNMQGGLLKWDAEGLSKPTDRIIGVCPQEFAELLNTDKKVLIDFYAEW
                     CAPCKKMTPYLLKMQKEMADKVAIIRLNADENPTLIKEMKILELPTLIIYENKVEKWK
                     NSGYISEEDLKKQLQ"
     gene            2734..3408
                     /locus_tag="PLQ70_07545"
     CDS             2734..3408
                     /locus_tag="PLQ70_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HQE34505.1"
                     /translation="MLSKNSLEFLDDLKANNNRDWFLDNKKRYEAFKKDYHQLVSDFL
                     SVMKPLDPSLEMLEVKNCTFRINRDIRFSKDKSPYKDHIGVWLSSGAKGKNRSGYYVH
                     IARRGSFIAGGFYCPEAEDLKKVRKEIAFFYEDLEEILNEKNFKKEFTDFDRNEINLL
                     KNPPRGYEKDHPAIEFLKLKSFETSQKFDIEEVTQKDFVAKMCQKLIALKPLNDFINR
                     ALTSED"
     gene            3417..4484
                     /locus_tag="PLQ70_07550"
     CDS             3417..4484
                     /locus_tag="PLQ70_07550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE34506.1"
                     /translation="MEKRKILFLGETYRADAITWMNGLKEFGDFEIITWELKTPNTTF
                     FSRLFRIFEFTTAPFQIRKRIKKEHPDMIIAERTTSYGFLAALSGVKPVAIAQQGRTD
                     LWPENSILLPLKIIIQNYAFKKADLIHAWGPVMTISMRKANVDMQKVLVMPKGIDLQQ
                     FGNKENTNSEKITAIVTRSLMPEYCHDTILKAFGILKQKGIDFKLTIVGDGKLLPALK
                     KLATNLNIDDKINFTGRIPNTELPELLQQSNFYISMPITEGVSASLFEAMASNCYPVV
                     TDIPGNQSWITHRENGQLIPIDNTKMLADELLWAFENKEHRNLAVQKNRKFVEENADY
                     KMNMKIIAKKYHQLINTSKTN"
     gene            4487..6334
                     /gene="asnB"
                     /locus_tag="PLQ70_07555"
     CDS             4487..6334
                     /gene="asnB"
                     /locus_tag="PLQ70_07555"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HQE34507.1"
                     /translation="MCGINGILHLQSQKKVDERILTKMRDALEHRGPDDKGLFIENNI
                     GLGHRRLSILDVSTAGHQPFLSDDGRYILVYNGEIYNFKDFYPELRSHGFDIKTSSDT
                     EVLLKLFQLYGLKMLHRLNGMFAFAIWDKTERKLIAVRDRMGVKPLYYSFYNETFYFA
                     SEQKALFTAGVPLKMAQDGMEEYIFNRFVAGENTLYENVKKILPGHILTIHESGKITN
                     EKWWDLKTEIQNQPKIKNPVEWFRETFDNSVQLRMVSDVPVGVLLSGGLDSSSILASL
                     NQQKFSHIQTFNIGFKEKEHNESHLAQRMSEKFGYTFHTMQLENKDLFEKLISATYFQ
                     DEPIMHLSEPHILALAQLAKPSVKVLLSGEGADELMGGYVRYKALKYPSLLKIIATVG
                     HMDYFTTKPRFEKLARYSQIKEKDDLILYNGSNIYPKDIEKTFGITNQPKNEYRNQIL
                     EDAKSLYPNNSRRQALYFDQHTYLCSLLDRNDRCTMGASIECREPFLDQRLIAGLGSL
                     EDQWLFTGKKWKYILKEAMKSRLPHEILHFKKVGLSVPWGDYLLKSPGFIDELESFSK
                     SDLFKMSYFENIDSQKLVSNLRKGDTKMIPFIMPMFMMHIWLKNYPNKF"
     gene            6375..>7194
                     /gene="murB"
                     /locus_tag="PLQ70_07560"
     CDS             6375..>7194
                     /gene="murB"
                     /locus_tag="PLQ70_07560"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQE34508.1"
                     /translation="MEIQTNFSLKNYNTFGIEAKAKLFVAVHSIAELKTVLEENKSQK
                     KFILGGGSNMLLTQDIDALVIHVDLKGKKIIKDDDNFVWVESQAGENWHEFVLWTIDQ
                     NFGGLENMSLIPGNVGTTPVQNIGAYGTEIKDTFVSCEAMAIENQKTKTFSKDECHFG
                     YRESVFKNEVKDQYIITSVVFKLTKRNHKINISYGDIAAQLSNKNIQIPTLKDVSDAV
                     IAIRTSKLPDPKELGNSGSFFKNPILLKSDFEKIHQQFPEMKYYDISPTEVKVPA"
BASE COUNT         2561 a         1274 c         1193 g         2166 t
ORIGIN      
        1 tattagattc agcagctttt acataataat ccaaagcttc ttttggttga tttttttgcg
       61 aataagcatc tccaattgca ccagttgcca tagcacttat tacaagatca tccgatttga
      121 attttcctaa atattcaatc gcttcagcat attttcctgt attcaaataa gcaataccag
      181 cataatagtt cgctaaattt ccagcatcag ttcctgaata ttcatcggca attttcacaa
      241 atccccattt tccttcagaa ccattcaagg ctaatttata taacgaatca cttgctacac
      301 catttgttgc tttttcaaaa tcttgttgtg ctacaaacat ttggttagct gcatcatcct
      361 cttttggcgc tgctacaaat ttttggtaag ctaaatatcc aacagttaat aaagctattg
      421 caccaacaac tccaataata attttttgat ttttggctac aaaatcttca gtttttgaag
      481 cagttgcatc caaagttgag aaaacttcag cggtagtact attcttttct aattctgcta
      541 cattgatatc ctctgtaact tctttaactt ctttttcttt tgctggttta tatcctcttt
      601 tattataagt agccatttaa attttaattt agtgagccgc aaaaataaaa tttttatttg
      661 tatagtcaca aaaaaaatgt ctttatattc aaataaaaat acattatcct ttttttatac
      721 tcttgattct tccgatttga aaaatatttg tccattgaaa ttgcctaaaa gtaattttta
      781 tcgatatttt tcgataattt gcaccctctt aaaaacaata cgaaaggcct gattttctta
      841 tacccgcaag actaaaaatg tatttaaaaa aaatttcact attcaattac aagaactttt
      901 cggaagccaa tttcgaattt gtaggcaaaa taaattgttt tgttggcaaa aacggaattg
      961 gaaaaaccaa tgttttggat gctatttatc atttgtctta cggaaaaagt tatttcaatc
     1021 cgctggcggt tcaaaacatt aaacacggcg aagatttttt tgttattgat ggcgaatttg
     1081 aaatcaaaga aagaaacgaa caaattattt gtagcctcaa aaaaggacag aaaaaaatcc
     1141 taaaacgcaa cggcaaaatc tatgataaat tttcagaaca tatgggattc attcctttgg
     1201 taatcatctc gccagccgac accgatttga ttgttgaagg cagcgaaact cgccgaaaat
     1261 ttatggatag cgttatttcg caattggatt ctaactattt aaaacatctc attcaatatc
     1321 aaaaagtgat aagtcagcgc aacgctttgt tgaaatattt tgcattgaat tttgttttcg
     1381 acaacgacac gctttctata tacaatgagc aactcaacac ttttgggcaa tacattttcg
     1441 aaaaaagaaa agaattcatc gaacaattta ttcctatttt caactcccac catcaggcca
     1501 tttcgggttc gcaagaaacg gttcagttgg tttatgaaag ccacttattc gagaatgatt
     1561 tattgactct tttggaagaa aacataagca aagatagagc tttacattat acgagcgtag
     1621 gaattcacaa agacgattta tcgtttgaaa ttgataaata tcccataaag aaatttggtt
     1681 ctcaaggcca acaaaagtca tttttaatcg cattgaaatt agctcaattt gagtttttaa
     1741 aaactcaaag tggtcaaaaa cctattttat tatttgatga tatttttgat aaactagacg
     1801 aaaatcgggt agcaaaaatt gtagaaatgg tcaatagcga cacttttgga caattattta
     1861 tctctgacac acatcccgaa agaacagaaa aaattgtcaa atctacccat caagtttata
     1921 aaattttcaa cctttaaaac ggtagtattt taactttaag tttcaaaatc aaagaaagaa
     1981 attgcctatt ttagcctttt aaaaatcaaa attataccct aatgaaactc ctttcaaaat
     2041 tctgtatatt attcttatcg ctatcgatag tttcctgcaa tggacaaaat gctaaaaata
     2101 taaaaaacct tgaaccaaag gctttttcag aaaccctaaa gacgactaca aacgtccaaa
     2161 tcatagacgt tagaacccct caagaatttg ccgcaggaca tcttgaaaac gcccaaaaca
     2221 tcgattggtt gggagataat tttgatgcaa ctattcagaa attagacaaa accaaacctg
     2281 ttttcatcta ctgcaaaacg agcaatcgaa gttcgcaagc tgctgaaaaa ttagaaaaat
     2341 tagggttcga aaacatctat aatatgcaag gcggactatt aaaatgggat gccgaaggac
     2401 tttctaaacc aaccgaccga attattggcg tttgcccgca ggaatttgcc gaactattaa
     2461 acaccgataa aaaagtctta atcgattttt atgccgaatg gtgtgcacct tgcaaaaaaa
     2521 tgactcctta ccttttgaaa atgcaaaaag aaatggctga caaagtcgca atcattcgat
     2581 tgaacgccga tgaaaaccct actttaatta aagagatgaa aattttagaa cttcccaccc
     2641 taattatcta tgaaaataaa gtggagaaat ggaaaaattc gggatacatt agcgaagaag
     2701 atttgaaaaa acaactgcaa taattcatta aatatgcttt caaaaaatag tttagaattt
     2761 cttgacgatt taaaagccaa caacaatcgc gattggtttt tggataacaa aaaaaggtat
     2821 gaagctttca aaaaagacta tcatcaattg gttagtgatt ttctaagcgt tatgaaacca
     2881 ctcgaccctt ctttagaaat gcttgaagta aaaaattgca cctttagaat caatcgtgac
     2941 attcggtttt ctaaagacaa atcgccttac aaagatcata ttggtgtttg gttgtccagc
     3001 ggtgcaaaag gcaaaaatcg ttctggctat tatgttcaca ttgctagaag aggtagtttt
     3061 attgcaggcg gattctattg cccagaagcc gaagatttga aaaaagtgcg caaagaaatt
     3121 gcctttttct atgaggattt agaagaaatt cttaatgaaa aaaacttcaa aaaagagttt
     3181 accgattttg acagaaacga aataaaccta ttaaaaaacc cgcccagagg ttacgaaaaa
     3241 gaccatccgg ctattgaatt tttaaaatta aaaagcttcg aaacctctca aaaattcgac
     3301 atagaagaag tgacccaaaa agattttgtt gccaaaatgt gccaaaagtt aatagctttg
     3361 aaaccattga atgatttcat caaccgtgca ttaacttctg aagattaatg atatcaatgg
     3421 agaaacgtaa aatacttttt ctgggagaaa cctatcgagc cgatgccatt acgtggatga
     3481 atgggttgaa agaatttggc gattttgaaa ttatcacttg ggaactcaaa acacccaaca
     3541 ccactttttt tagccgatta tttcgaattt tcgaatttac tacagctcct ttccagatta
     3601 gaaaacgaat caaaaaagag catccagata tgattattgc cgaaagaacc actagttatg
     3661 gttttcttgc agcattatca ggcgtaaaac cagtagccat tgcgcaacaa ggcaggaccg
     3721 atttgtggcc agaaaattcc attttgttac cactcaaaat aatcattcaa aattatgctt
     3781 tcaaaaaagc cgatttgatt cacgcctggg gacctgtaat gaccatttcg atgcgaaaag
     3841 ccaatgtcga tatgcaaaaa gtgttggtta tgccaaaagg aatcgatttg caacaatttg
     3901 gaaataaaga aaatacaaat tcagaaaaaa ttacagcaat tgtaactcgg tcgttaatgc
     3961 ctgaatactg tcatgacact attctgaaag cctttgggat cttaaagcaa aaaggaattg
     4021 attttaaatt gaccattgtt ggcgacggaa aattgcttcc tgccctaaaa aaattggcaa
     4081 cgaatttaaa cattgatgac aaaattaatt ttacaggaag aattccaaat acagaactac
     4141 ccgaactttt acaacagtct aatttttaca tcagtatgcc tataaccgaa ggggtttcgg
     4201 cttccttatt tgaagcaatg gcttctaatt gttatcctgt tgtgaccgat attccgggaa
     4261 atcaaagttg gattacccat cgtgaaaacg gtcagttgat tccaatagat aacaccaaaa
     4321 tgcttgccga tgaactcctt tgggcttttg aaaataaaga acaccgaaat ttggcagttc
     4381 aaaaaaacag aaaatttgta gaagaaaatg cagattataa aatgaatatg aaaatcattg
     4441 ccaaaaaata tcaccaatta attaacacat ccaaaaccaa ctaactatgt gtggaattaa
     4501 tggtatctta catttgcaat cacaaaaaaa agtagatgaa cgcattctaa caaaaatgcg
     4561 ggatgcacta gaacatcgtg ggccagatga caaaggatta tttatcgaaa ataacattgg
     4621 tttggggcat cgtaggcttt ctattttaga tgtttcgacg gctggacacc aaccttttct
     4681 atccgatgac ggtcgctata ttttggttta taatggcgaa atatacaatt tcaaggattt
     4741 ttatcccgaa cttcgaagtc atggttttga cataaaaact agttcagata ctgaagtttt
     4801 attgaaatta tttcagttgt atggtttaaa aatgctacat cgccttaacg gaatgtttgc
     4861 ctttgcgatt tgggataaaa cagaacgaaa actgattgcc gttcgggata gaatgggcgt
     4921 taaacccttg tattattctt tttacaacga aacattttat ttcgcttctg aacaaaaagc
     4981 actttttacg gcaggagttc ctctaaaaat ggctcaagac ggaatggaag aatacatttt
     5041 taaccggttt gtagctggcg aaaatacttt gtatgaaaat gtcaaaaaaa tacttccggg
     5101 acacattttg accattcacg aaagtggcaa aattactaat gaaaaatggt gggatttgaa
     5161 aacagaaatt caaaaccaac ccaaaatcaa aaatcctgtc gaatggtttc gagaaacatt
     5221 tgacaattca gtacaattgc gaatggtgag tgatgttcct gttggcgttt tactcagcgg
     5281 cggactcgac tcttcctcta ttttggcgtc cttaaaccaa caaaaattta gccatattca
     5341 aacctttaat attggtttca aagaaaaaga acacaacgaa tcgcatttgg cacaaagaat
     5401 gtccgaaaaa ttcggctaca cttttcacac catgcaatta gaaaataagg atctttttga
     5461 aaaattaatc agtgctactt attttcagga tgagcccata atgcatttga gcgaacctca
     5521 tattttggca ttggcccaat tggcaaaacc atcagtaaaa gtgttgcttt caggagaagg
     5581 cgccgatgaa ttaatgggag gttatgtgag atacaaagcg ctgaaatacc cttcattgtt
     5641 gaaaataatt gcaacagtcg gtcacatgga ttatttcaca accaaaccaa gattcgaaaa
     5701 attagcgcgt tattctcaaa taaaagaaaa ggatgatttg atactttata acggttctaa
     5761 tatttatcca aaagacattg aaaaaacctt tggaatcaca aatcagccta aaaacgaata
     5821 ccgaaatcaa attctagaag atgcaaaatc attgtatccc aataattcga gacgacaagc
     5881 cctttatttt gaccaacaca cttatttatg ttcactactc gaccgaaacg accgttgcac
     5941 gatgggagct tctatcgaat gtagagaacc ttttctagac caaagactta ttgccggatt
     6001 gggttcatta gaagaccaat ggctttttac tggaaaaaaa tggaaataca ttctaaagga
     6061 agctatgaaa tcgcgattgc cccatgaaat cctacatttc aaaaaagtgg gtttgagtgt
     6121 tccttggggt gattatttac taaaaagtcc aggattcatt gacgaattag aatcgttttc
     6181 aaaaagtgac ttgttcaaaa tgtcgtattt tgaaaatatc gattctcaga aattagtttc
     6241 aaatttgaga aaaggagata ccaaaatgat tccgtttatt atgcctatgt ttatgatgca
     6301 tatttggcta aaaaactatc caaataaatt ttaatactta cttttggact aacgattcaa
     6361 aacgcacttt ctttatggaa atacagacta atttttctct taaaaactac aatacttttg
     6421 gcattgaagc caaagccaaa ctatttgttg ccgttcattc aattgcagaa ttaaaaaccg
     6481 ttttagaaga aaacaaatcc cagaaaaaat tcattttggg tggcggaagc aatatgcttt
     6541 tgactcaaga tatcgatgca ttggtgattc acgttgattt gaaaggtaaa aaaatcatca
     6601 aagacgacga taattttgtt tgggtcgaaa gtcaagctgg cgaaaactgg cacgaatttg
     6661 tactttggac catcgatcaa aactttggag gattggaaaa tatgtcgctg attccaggaa
     6721 atgttggaac cactccagtt cagaatattg gcgcatatgg aaccgaaatc aaagacactt
     6781 ttgtttcttg cgaagcgatg gcaatcgaaa atcaaaaaac caaaactttt agtaaagacg
     6841 aatgccattt tggctacaga gaaagtgttt tcaaaaacga ggtaaaagac caatatatta
     6901 tcacttcggt agtttttaaa ctcaccaagc gcaaccataa aattaatatt tcctatggcg
     6961 atatagctgc ccaactttca aataaaaaca tccaaatccc aactcttaaa gatgtttctg
     7021 atgctgtaat cgctattcga actagtaaat tgcccgatcc aaaagagtta ggaaacagcg
     7081 gaagtttctt taaaaacccc attcttttaa aatcagattt cgagaaaata catcagcaat
     7141 tcccagaaat gaaatactat gatatttctc caaccgaagt caaagtccct gcgg
//
LOCUS       DAOSNN010000130         7188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10418_length_7188_cov_7.689612, whole genome shotgun sequence.
ACCESSION   DAOSNN010000130 DAOSNN010000000
VERSION     DAOSNN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10418_length_7188_cov_7.689612"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1160
                     /locus_tag="PLQ70_07565"
     CDS             144..1160
                     /locus_tag="PLQ70_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34509.1"
                     /translation="MKKKLEADLMSIAHRVLQMKNKSDINQLCVETQKLYEKLAVLRF
                     VEEHFGGAKPTIGQAEVVAKMKQFFEENHLSEFKPDTSKIEIVAEEEINEEDDTIAEE
                     EILEASDAEFNEIEPAEEEEVQEEIIENLEEEVVNDDIVLDAIEAETETDFNETEPDE
                     EGEEVLEEEPTNPVELGFTPAFELDTEEEAEIATPKKVEAIQISFEELLGANYSETLF
                     VKAEPKKELTSPMDFEIPKEIVSKVDISIPEKNETKTETINDKLATGISIGLNDKIAF
                     VKNLFGNSDEDYSRVINQLITFNNFEEAQSFIEEMVKPDYSNWEGKEDYEKRFMDIIE
                     KKFV"
     gene            1161..1874
                     /gene="rsmI"
                     /locus_tag="PLQ70_07570"
     CDS             1161..1874
                     /gene="rsmI"
                     /locus_tag="PLQ70_07570"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQE34510.1"
                     /translation="MGKLYIVPTPIGNLEDMTFRAIRILKEVDLILAEDTRTSGKLLK
                     HFEIGTHMHSHHMHNEHKTVENLITRLKAGETIALISDAGTPAISDPGFLLTRACVEN
                     KIDVECLPGATAFVPALVNSGLPNDRFIFEGFLPEKKGRQTRYLALAEETRTMILYVS
                     PHKLVKTLAEFITYFGEDRQICVSRELSKLHEENVRGTTREVLTHFENKPPKGEIVVV
                     VAGKTITKENKSSRTESPE"
     gene            2025..3743
                     /gene="recJ"
                     /locus_tag="PLQ70_07575"
     CDS             2025..3743
                     /gene="recJ"
                     /locus_tag="PLQ70_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQE34511.1"
                     /translation="MRWNIKPKPSGEKVKLLAEALNVEEFVATLLVQRGIETFEQARQ
                     FFRPTLADLHNPYLMKDMDKAVERIEKAIENGENILVFGDYDVDGTTAVSLVSSYLKT
                     YYPNVATYIPDRYDEGYGVSFKGIDFADDNGFSLIIALDCGIKSIDHVAYAKDKNIDF
                     IICDHHRPGEFLPEAVAVLDPKRDDCNYPYDELCGCGIGFKLIQALGENRNQTIEDLT
                     PYLDLVATAIAADIVPMTGENRVLAYFGLQVINADPRPGFKALITSSVRPSVSGHQIK
                     KKTLDITDVVFIIAPRINAAGRIKHGNHAVELLTEFDFEQAQQFALEIEAYNSERKDL
                     DKQITKEALNQIIENNEEKRFTSVVFQEDWHKGVIGIVASRLIETYYRPTLVFTKSGD
                     KYAASARSVKGFDVYNALEACSEHLDQFGGHMYAAGMTLREENYQNFKNAFEKVVEAT
                     IHPDMLTPEISIDAEINLSDITPKLTRILKQFEPFGPQNMTPVFLTKNVKDTGYAQKL
                     GADEEHLKLFVRQNNSEGMATIGFGLGNKMELTTNKKPFEAVYCIDENEWNGKTSIQL
                     RLKDIR"
     gene            3864..>4891
                     /locus_tag="PLQ70_07580"
     CDS             3864..>4891
                     /locus_tag="PLQ70_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34512.1"
                     /translation="MNEKIRDPYAALRYKEFNLFLVLRFAMVFAWSMQFIIIEWEVYS
                     LTKSALSLGMIGLMEVIPAIAMALFAGHIVDQKEKKGMLVKCIFGFSIISFGLFLLTW
                     PRIVTHWSTDTILYSIYFLVFLGGLVRSFLGPTIFSLLSLIVPKKVYPNAATWSSSVW
                     QIGSVVGPAVAGFSITWIGVHWSMCSIFACSVFSLLILTQISKKPILNPKIGEPVMES
                     LKEGIKFVYTNKSVLGALTLDMVAVLFGGAVALLPIFAQDILKVGPEGFGILRAAPAV
                     GALLTMFVSAHLPFYKNAGIKLLAAIFGFGVCIIVFGLSTWFWLSVFALFMSGVTDGI
                     SVVIRQTI"
     assembly_gap    4892..4991
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4992..5345
                     /locus_tag="PLQ70_07585"
     CDS             <4992..5345
                     /locus_tag="PLQ70_07585"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HQE34513.1"
                     /translation="LISTIPILGVSTFLITTVSLKRKLNLPVMIALSYLMWPIQILLI
                     IPFIRVGEFIFSVPRNHHTVDEIISSFQNSFFQTLSHLSFELLCGLGGWLLTAVPLAV
                     VIYWVSLLFLKKAGK"
     gene            complement(5355..5816)
                     /locus_tag="PLQ70_07590"
     CDS             complement(5355..5816)
                     /locus_tag="PLQ70_07590"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HQE34514.1"
                     /translation="MNANIEIKEIPTLELAFITQIGHEGLGNAYAKLIQWAAPKGLLK
                     EDSKMATIYHDSFKITDPDKVRMSACLILNEKTEVSGEIGLTTIEKGKFIVGHFEIGI
                     HEFEKSWTGLFIWMNENGYKKAEQNPFEIYHNNFNEHPEKIAIVDFCIPVE"
     gene            complement(5909..6670)
                     /locus_tag="PLQ70_07595"
     CDS             complement(5909..6670)
                     /locus_tag="PLQ70_07595"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQE34515.1"
                     /translation="MQITPNKKIYFASDQHFGAPTPELSFPREQKFVAWLDEVKKDAE
                     VIFLLGDLFDFWFEYKTVVPKGFVRVLGKLAEIRDSGIPIYFFVGNHDLWMNDYLEKE
                     LNIPVYHDNQEYTFNGKTFLVGHGDGKGPGDKGYKRMKKVFTNPFSKWLFRWLHPDIG
                     VKLGHYLSVKNKLISGDEDVKFLGEENEWLILYSKRKLETKHYNYLVFGHRHLPMIVP
                     VGENAEYVNLGDWISYFTYGVFDGETFEVKKFETK"
     gene            complement(6694..7146)
                     /locus_tag="PLQ70_07600"
     CDS             complement(6694..7146)
                     /locus_tag="PLQ70_07600"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQE34516.1"
                     /translation="MSKIRITKQFGFETGHALYGYDGKCKNVHGHSYKLSVTVIGTPI
                     TDRSNAKYGMVIDFTDLKKIVKEEIVDQFDHASVFNGTTPHIELANELKSRGHHVILV
                     DYQPTSENMVVDFSKRIIGRLPENIKLFSLKLQETESSFAEWFASDNL"
BASE COUNT         2186 a         1237 c         1420 g         2245 t
ORIGIN      
        1 accaaattgt tctttatgat ttactgtatt ttcgagaaac attttgtatt tttctgccct
       61 taaaaaggat tagtttttat tactttgtac agaaacaaat ttattaaaaa agacatacct
      121 tactcactat aatttcaaaa gttatgaaga aaaaattgga agccgactta atgagcattg
      181 cacaccgagt gttgcaaatg aaaaacaaat cagacatcaa tcaattgtgt gttgaaacgc
      241 agaaattata tgaaaaactg gctgttttaa gatttgtcga agagcatttt ggaggagcaa
      301 aaccaactat tggtcaagcc gaagttgtcg caaaaatgaa acaatttttc gaagaaaatc
      361 atttgtcaga atttaaaccc gatacttcaa agatagaaat tgttgctgaa gaagaaatta
      421 atgaagagga tgacaccatt gcggaggaag aaattctaga agcttcagat gctgaattca
      481 atgaaataga accagccgaa gaggaagaag ttcaagaaga gattatagaa aatcttgagg
      541 aagaagttgt caatgatgac atcgttttgg atgctattga ggctgaaact gaaactgact
      601 ttaatgagac agaacccgac gaagaaggtg aagaagtttt agaagaagaa ccaacaaacc
      661 cagttgaatt aggatttaca cctgcttttg aattagacac agaagaagag gcagaaattg
      721 ctactcctaa aaaagttgaa gcaattcaga tttcgtttga agaattattg ggtgccaatt
      781 attctgaaac actttttgtg aaagccgaac ccaaaaaaga attgacatct ccgatggatt
      841 ttgagattcc aaaagaaatc gtttcaaaag ttgatatctc tattcctgaa aagaatgaaa
      901 ccaaaacaga aaccataaat gacaagcttg ctactggaat cagtattggc ttaaatgata
      961 agattgcttt tgtgaaaaat ctttttggta atagcgatga agattatagt cgtgtgataa
     1021 atcaattaat tactttcaat aattttgagg aagcccaaag ttttatcgaa gaaatggtaa
     1081 aaccagatta cagtaattgg gaaggcaaag aagattatga aaaacgtttt atggatatca
     1141 tcgagaaaaa attcgtttaa atggggaaac tatatattgt tccaactcca attgggaatc
     1201 ttgaagatat gacttttcga gccattagaa ttctaaaaga agtagatttg attttggcag
     1261 aagacacccg aactagtgga aaattattga aacattttga gattggcacg cacatgcaca
     1321 gccatcacat gcacaacgaa cacaaaacag tagagaattt aattactcgt ttgaaagcag
     1381 gagaaaccat tgccttaatt tcagatgcgg gaactccagc catttcagat ccaggatttt
     1441 tgttgactcg tgcttgtgtc gaaaacaaaa ttgatgtgga atgcttgcca ggtgcaaccg
     1501 cttttgtacc tgctttagtc aacagcggat tgccaaatga cagattcatt ttcgaaggat
     1561 tcctgccgga aaagaaagga agacaaaccc gatatttggc gctcgccgaa gaaacccgca
     1621 caatgatttt gtatgtttca ccacataaat tagtcaaaac tttagcggaa tttattacct
     1681 attttggcga agatcgccaa atttgtgttt ccagagaatt atccaaattg cacgaagaaa
     1741 atgttcgtgg aacgactcgt gaagttttga ctcattttga aaataaaccg ccaaaaggag
     1801 aaattgtggt ggtggttgca ggaaaaacaa tcactaaaga aaataaatca tcaaggactg
     1861 aaagtccaga ataaaaaata tttcaccgca gattcgcaga ttttttcaat gattttataa
     1921 aatttgcgaa tttgctatta ttttttttgc acttaaattc tttagctatt ttaaactaaa
     1981 tttgtgagag tacaaaaaac ctaattctca attcgaaatt ttatatgcgc tggaacatca
     2041 aaccaaagcc ttcgggagaa aaggtaaaac tacttgctga agctttgaat gtggaggaat
     2101 ttgtggcaac gcttttggtg caacgaggta ttgaaacttt cgaacaagcc cgacaatttt
     2161 ttcgtcccac tttagcagat ttgcataatc cgtacttgat gaaagatatg gacaaggcgg
     2221 tcgaacgtat tgaaaaagcg attgaaaacg gcgaaaatat tcttgttttt ggcgattatg
     2281 atgtcgatgg aaccactgcg gtttccttgg tttcgtctta tctgaaaacc tattatccca
     2341 atgtggcgac ttacattcca gatcggtatg acgaaggtta tggcgtttct ttcaaaggaa
     2401 tcgactttgc cgacgacaat ggtttttcgc tcataattgc tttggattgc ggaataaaat
     2461 ctatcgatca cgtggcttac gccaaagata aaaatatcga ttttattatt tgtgaccatc
     2521 accgtccggg agaattttta cccgaagcgg ttgccgtttt agatccaaaa cgagatgatt
     2581 gcaattatcc ttacgatgaa ttatgtggtt gcggaattgg ttttaaattg attcaggctt
     2641 tgggtgaaaa ccgaaaccaa accatcgaag atttaactcc gtatttagat ttggtggcaa
     2701 ccgcaattgc tgctgatatt gttcctatga cgggagaaaa tcgtgtttta gcgtattttg
     2761 gattgcaagt aattaatgca gatccaagac caggatttaa agctttaatt acttcgtccg
     2821 ttcgaccttc ggtctcgggt catcaaatca aaaagaaaac tttagatatt accgatgtcg
     2881 tttttatcat cgcaccccga attaatgcag caggacgcat caaacacggg aatcacgcag
     2941 tggaattgtt gactgaattt gatttcgaac aagctcaaca atttgcctta gaaattgaag
     3001 cctataattc cgaaaggaaa gatttggaca agcaaattac caaagaagca ttaaatcaaa
     3061 ttatagaaaa taacgaagaa aaacgattca cctcggtggt ttttcaggaa gattggcaca
     3121 agggcgtgat tggaatcgtg gcttctagat tgatagaaac ctattaccgt ccgaccttgg
     3181 tttttaccaa aagtggcgat aaatacgcag cttcagcacg atctgtaaaa ggttttgacg
     3241 tttacaatgc cttggaagct tgttcggaac atttggatca atttggcgga catatgtatg
     3301 cagctggaat gactttacga gaagaaaatt atcaaaattt taaaaatgct ttcgaaaaag
     3361 ttgtcgaagc aaccattcat cctgatatgt tgacaccaga aatttcgata gatgctgaaa
     3421 ttaatttatc ggatattacg ccaaaactaa cccgaatttt gaaacaattt gaaccttttg
     3481 gcccccaaaa tatgacgccc gttttcttga ctaaaaatgt gaaagacacg ggttatgccc
     3541 aaaaattggg agctgacgaa gaacatttga aactttttgt acgccaaaat aattcggaag
     3601 gaatggcgac cattggtttt ggattaggaa ataaaatgga attgacaacc aataaaaaac
     3661 cgtttgaagc ggtctattgc attgatgaaa atgaatggaa tggcaaaaca tccatccaat
     3721 tgcgattgaa agatattaga taagaaggca gatttcagaa atccaaaacg gacaacgaaa
     3781 accaaaattc gttatccgtt gctcgacaac cgattaacga acaacgaaaa tcgagcaacg
     3841 aaaaaatatt tgaataatag tttatgaatg agaaaataag agatccctac gcagccttgc
     3901 gttacaaaga atttaatctg tttttggtgt tgcgatttgc aatggttttt gcttggtcaa
     3961 tgcagtttat catcattgaa tgggaagtct atagtctgac caaaagtgcc ctttcactgg
     4021 gaatgattgg attaatggaa gtgattccag ccattgcaat ggctttattt gcgggacata
     4081 ttgtcgatca aaaggagaaa aaaggaatgt tggtcaaatg tatttttggt ttttccatca
     4141 ttagttttgg cttgttttta ttgacctggc cgagaatagt aacccattgg tcaaccgata
     4201 cgattttata ttctatttat tttttagtgt ttttaggagg cttggttcgt tcttttttgg
     4261 ggccgaccat tttttcgctt ttgtccttga tagttcccaa aaaagtctat cccaatgcag
     4321 ccacttggag cagttcggtt tggcaaattg gttcggtcgt tgggccagcc gtggcgggat
     4381 tttccattac ttggataggc gtgcattggt cgatgtgttc catttttgcc tgttctgttt
     4441 tttcattatt gattttgaca caaatttcta aaaagcctat tttgaatcca aaaatcggag
     4501 aacccgtgat ggaaagtttg aaggaaggca tcaaatttgt ttacaccaac aaatccgttt
     4561 tgggagcttt aacgctcgat atggtggcgg ttctttttgg tggcgccgtg gcacttttac
     4621 ccatttttgc ccaagatatt ttgaaagttg gccctgaagg ctttggtatt ttaagagctg
     4681 cgccagccgt tggagcttta ttgacgatgt ttgtttcggc gcatttgcct ttttataaaa
     4741 acgcgggaat caaattgttg gcagctattt tcggattcgg tgtttgtatt attgttttcg
     4801 gactttccac ttggttttgg ctctcggttt ttgctttgtt tatgagcggt gttaccgatg
     4861 gaatttcggt ggtgattcgc cagacgattt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nttgatttcg accattccca ttcttggcgt gagcactttt ctgattacca
     5041 cggtttccct caaacgaaaa ttgaatttac cagtaatgat tgcactcagt tatttgatgt
     5101 ggccaattca aattctattg attattcctt ttattcgagt gggtgaattt attttttccg
     5161 tccctcgaaa tcatcatacg gtcgatgaaa ttatcagttc gttccaaaac agttttttcc
     5221 aaaccctgag ccatctttct tttgaattac tctgcggttt gggtggttgg ttgctgactg
     5281 cagttcctct tgcagtggtg atttattggg tgtcgctttt gtttttaaaa aaagctggca
     5341 aataacaaaa tattttattc tacagggatg caaaaatcaa caatggcaat tttttcagga
     5401 tgttcattga agttgttgtg gtagatttca aacggatttt gttctgcttt tttatatcca
     5461 ttttcgttca tccaaatgaa aagacccgtc caggattttt caaattcgtg gattccaatt
     5521 tcaaaatgac ccacaataaa cttccctttt tcgatggtgg tcaagccaat ttcaccgcta
     5581 acttcggttt tttcgtttaa aatcaggcaa gcgctcatcc tgactttatc gggatcggtg
     5641 attttaaaac tgtcgtgata aatggtagcc attttagagt cttccttcaa aagccctttg
     5701 ggagcagccc attgaatgag tttggcgtaa gcatttccca aaccttcgtg tccaatttgt
     5761 gtaatgaaag ccaattctaa tgttggtatt tccttgattt caatatttgc attcattttc
     5821 ttggggttct aaagattgcc tgttaattta gttttgcttt tgaatgagaa aaatttaatt
     5881 aaaacttata tatgcctatg tggtaaaatt attttgtttc aaatttcttc acttcaaaag
     5941 tttcgccatc aaaaacgcca tacgtaaaat aactaatcca gtcgcctaga ttgacatatt
     6001 cggcattttc gccaacagga acaatcatcg gtaaatgacg gtgaccaaaa acaaggtaat
     6061 tgtaatgttt ggtttctagt tttcttttgg aataaagtat caaccattcg ttttcttcgc
     6121 caaggaattt cacgtcttca tcgccagaaa tcagtttgtt tttcaccgat agataatgtc
     6181 ccagttttac gccaatgtcg ggatgaagcc atcgaaacaa ccattttgaa aaaggattgg
     6241 tgaagacttt tttcattcgt ttgtaaccct tgtcgccggg tccttttccg tcgccgtgac
     6301 ctaccaaaaa agtttttccg ttgaaagtat attcttgatt gtcgtggtaa accgggatgt
     6361 tgagttcttt ttcgagataa tcgttcatcc acaaatcgtg gttacccaca aagaaataaa
     6421 taggaatgcc gctgtcgcga atttcggcca atttgcccaa aacgcgcaca aatcctttgg
     6481 gaacaacggt tttgtattcg aaccaaaaat caaataaatc acccagtaga aaaatcactt
     6541 cggcatcttt tttgacttcg tcgagccaag ctacaaattt ttgttctcta ggaaaactca
     6601 attcaggagt tggcgcacca aaatgctggt cggaagcgaa atatattttt ttgttgggcg
     6661 tgatttgcat aaaattgaaa tggagatttt gaattacaaa ttatcactcg caaaccactc
     6721 ggcaaacgaa gattcggttt cttgcaattt caaggaaaaa agtttgatat tttctggcaa
     6781 tctaccgata attctttttg aaaaatcgac taccatattt tcgctcgtag gttggtaatc
     6841 gaccaaaatt acgtggtgac ctcgggattt tagttcattg gccaattcaa tatgcggtgt
     6901 ggttccgttg aaaacagagg cgtggtcaaa ttggtcaacg atttcttctt tcacgatttt
     6961 tttcaaatcc gtaaaatcga taaccatccc atattttgca ttgctgcggt cggttatggg
     7021 cgttccaatt acggtaaccg acaatttata actgtgaccg tgcacgtttt tgcatttgcc
     7081 atcataaccg taaagggcgt gaccggtttc gaaaccaaac tgttttgtaa tccttatttt
     7141 gctcattcta tttttatttg agtgcaaatt tagtgaatct actccttt
//
LOCUS       DAOSNN010000131         7185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10424_length_7185_cov_3.976017, whole genome shotgun sequence.
ACCESSION   DAOSNN010000131 DAOSNN010000000
VERSION     DAOSNN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10424_length_7185_cov_3.976017"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..>572
                     /locus_tag="PLQ70_07605"
     CDS             274..>572
                     /locus_tag="PLQ70_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HQE34517.1"
                     /translation="MENLVKSLELKVGVWKDSINVHDFVLQNLKSYEGDASFLVGPSY
                     KTTQLWNVCKESLLKERQNDGCLAIDTEVISNITSFGPGYIQKENETIVGLQTDEL"
     assembly_gap    573..582
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            596..2032
                     /locus_tag="PLQ70_07610"
                     /pseudo
     CDS             596..2032
                     /locus_tag="PLQ70_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
     gene            2159..2518
                     /locus_tag="PLQ70_07615"
     CDS             2159..2518
                     /locus_tag="PLQ70_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HQE34518.1"
                     /translation="MHGRDENGAIASLNSVSKLNYNDAQDGISYTFTMVPKSLGNNLE
                     EQVGNLTTTLDAYFGRNAHHLNVNVLNKETLLDAYNHPENYPQLTIRVSGYAVNFVRL
                     SKAHQLEVISRTFHSTM"
     gene            2616..3392
                     /gene="pflA"
                     /locus_tag="PLQ70_07620"
     CDS             2616..3392
                     /gene="pflA"
                     /locus_tag="PLQ70_07620"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HQE34519.1"
                     /translation="MYFPEQDTHLLNESEIDLLRIHSLETFGTHDGPGIRMVVFVQGC
                     QFRCLYCQNPDSIDIHGGTLVTVDELVSKAVKQKAYFGKKGGVTVSGGEPLLQRAKLI
                     HFFDRLHENGINTCLDSNGRVLDDHTKELLDRTDLLLLDVKHINPEWHKKLTSLNNAT
                     TLKIAEHRESTGKPMWLRYVFVPGWTDQEEYLHEWAKHFSGYKTIERVEIIPFHQLGK
                     HKWELMNMQYALAETPSPTTEALENAKAIFSQYLDNVTIK"
     gene            3594..4763
                     /locus_tag="PLQ70_07625"
     CDS             3594..4763
                     /locus_tag="PLQ70_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HQE34520.1"
                     /translation="MKKNKYLIVLAGMVMQLSIGSIYAYSKWIEPLTTALNWDAHDTK
                     TGFSLAICFLGLTAAFMGKFAQKVGPTKAGIIATLFLTIGLLGTAYAVSVGNIYLFYL
                     TFGVLQGIGLGFGYVVPVYTVVKWFPEKPGMASGIIIMSFALGSLLASFLIGPLIASM
                     GLSGAFETLAVGYGICMLLSALYLSNPEITSNVHHAHIDLTPKQIITDKRFIALWLLL
                     FLNVCGGIAIISKAAVLGEEVAGMSAAQATLFVAIIGLFNGLGRLFWSSISDKIGCWA
                     TFMLFIAINVVCFALIPSFSTSLIAFQVLTFIVIAGYGAGFATMPSFVKSIYGAENYG
                     QVLGYTLTAWSAAAFVGPILLGLSTEITIFYLFSILLIIALIVGIWLKGLLVKTT"
     gene            complement(4890..7022)
                     /locus_tag="PLQ70_07630"
     CDS             complement(4890..7022)
                     /locus_tag="PLQ70_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQE34521.1"
                     /translation="MANFDIKHIKNVVLVGHTGSGKTTLIESMLFEAGLINRRGSILE
                     KNTVADYSEIEQEKGKSFFSKLVNTKWRGYKINLLDTPGYDDFAGEIIGAMRVADCGL
                     MLLNSSMGVEVGTDIIWEYANDYKLPILLAVNQLDNEKSDFDKTIQEAKDHFGQSVVV
                     VQYPLNQGANFDAVVDVLNMVLYKYPKNGGKPEKLPIPENEIEKANRLHQELIETIAG
                     NDETLMELYFEKGELNEDEMKLGLHHSLIKHEIFPVFCVSAKKNMGSGRIMGFIDNVC
                     PSAYEMPKQKTIDGKEIECSENAPTAIFIYKNLSEPHIGDLSFFKVLSGKIKVGDELT
                     NENTGQIERINQLYEVEGSKRTPVNELTAGDIGATLKLKDTHINNTLHEKGKHIEITP
                     IVFPNSTYSIAVEGTKKGEEEKLSMALHQLVEEDPTIRIEVSPELNQTIIHCHGELHL
                     AIIKWKLEKQYKLSVEFRSPKIPYRETIQKSAEITYRHKKQSGGSGQFAEVAMKIEPW
                     HEGMDEPKGYNIRGKEEINLPWGGKLIYYNCIVGGAIDTRFHPSILKGIMEKMTKGPL
                     TGSYVRDVRVILYDGKMHPVDSNDLAFKTAGMMAFKENFIKADPQLLEPFYHIKIQAP
                     EELTGAIMSNIQTHRANVESMDAEGQFTLITAFLPFAEMQEFGSDLRSLTQGRARFTA
                     KFDHYESVSFEMQKKLVSEFKKEEELVY"
BASE COUNT         2275 a         1197 c         1291 g         2412 t
ORIGIN      
        1 agactaagga cgtttttcag aagaaaatga gcaaattttg aaaaataaaa aaaactatac
       61 cgttatcgta aatattacaa ttttctttca aaaacatata aaaatttcac ataataaatt
      121 attgtgaccg taaagttgtt gtattgtctg taaattaagt gtgtaattga tacattccta
      181 aaaaatcagc gaaatacaca ttctatttac atttatcgta aatttaaaca tagaaaaaag
      241 atagttaaaa aagaaaaact aagaaatata aaaatggaaa atttagtaaa aagtttagag
      301 ttaaaagtag gagtttggaa agatagtatt aatgtacacg attttgtttt gcaaaacctt
      361 aaatcatatg aaggcgacgc tagttttctt gtagggcctt cttataaaac cactcaactt
      421 tggaatgttt gtaaagaatc acttttgaaa gaaagacaaa atgatggttg tcttgccatt
      481 gacaccgaag ttatttcgaa tattacttct ttcggacctg gttatattca aaaagaaaat
      541 gaaactattg ttggacttca aaccgatgag ctnnnnnnnn nntttaaaaa gagctatgaa
      601 accttttgga ggaatcaaat tagtacaatc ggcagttgct gaaaaaggct tgaaagtttc
      661 tgatagagta gtagatattt ttaattacgc caaaaatcat aatcaagcag tttttgatgc
      721 ttatgatggc gaaatcaaaa cctttagatc taaacacgta ttaacaggtt tgcctgataa
      781 ttacgcaaga ggaagaatta ttggtgattt taggagagtg gctctttatg gaattgacag
      841 attaattgca gagaagaaat cagacaaaga aaaagtgtct ggcgaaatga gcgatgctaa
      901 agttcgtttg cgtgaagaaa ttgcagagca aattaaagcc ttgcaggata tgaaaaaaat
      961 ggctctttct tatggaattg acatttctaa acctgcaaca aatgctcaag aagctgttca
     1021 gtttacatat ttagggtatt tgagtgctgt taaggaacaa gatggagctg caatgtctct
     1081 tggaaatgtt tcttcattct tagatgttta cattcaaaac gatatcgaag caggaattat
     1141 agacgaagaa caagctcaag aatatataga ccaatttgta atgaaattgc gtttggtgcg
     1201 tcatttgcgt ccaggtgctt atgatgctat ttttggtgga gatccaactt gggttaccga
     1261 agctatcgga ggtcaattcc atgacggtag aactaaagtg accaaaacat ctttccgttt
     1321 cttgcaaact ctttataatt taggtgcttc accagaaccc aatttaacgg ttttatggtc
     1381 tgaaaatttg ccacaagggt tcaaagattt ttgtgctcaa gtttcaattg acacttcatc
     1441 acttcaatat gaaaatgacg atttgatgag agacaaccgt ggctcagacg attatggaat
     1501 tgcttgttgc gtttcttatc aagaaattgg aaaatcaatt caacattttg gtgctcgtgc
     1561 caatttgcca aaagcacttt tattgtcatt gaatggtgga agagaagaat cagaaggagt
     1621 tcaagtagta ggaggaattt cagaaatgga tgacgaagta ttggattatg atactgtaat
     1681 gaaatcattc aagaaaacat tagccgaagt agctcgtgtc tatgcaaaat caatgtatat
     1741 cattcattat atgcacgata aatattacta cgaaagagct caaatgtcat taattgatag
     1801 taatcccaaa attgatattg catatggagc tgctggaatt tctatcattg ccgattcact
     1861 ttctgccatt aaatatgcta aagtaactcc agttagaaac gaatttggat tgactataga
     1921 tttcaatatc gaaggtgatt ttccaaaatt tggtaacgat gacgatagag tagatgacat
     1981 cgctaaagaa gttactaaga tttttatcga tgagttgaga aaacacaaaa cctataaaaa
     2041 ttctacacca acactttcct tgttgaccat tacatccaac gtaatgtatg gttctaatac
     2101 tggtgcaact cctgacggtc gtaaagcagg cgaagctttt gctcctgggg caaatccaat
     2161 gcacggtaga gatgaaaatg gagcgattgc ttctttaaac tcagtgagta aattgaatta
     2221 taacgatgct caagatggaa tttcttatac ttttacaatg gttccaaaat cattaggaaa
     2281 caatttagaa gagcaagttg gtaatttgac tactacactt gatgcttatt ttggaagaaa
     2341 tgcacatcac ttgaatgtga atgttctgaa caaagaaacc ttgttagacg catacaatca
     2401 ccctgagaat tatcctcaat tgaccatccg tgtttctggt tatgcagtaa attttgtgag
     2461 attgtcaaaa gcacatcaat tagaagtgat tagcagaact ttccatagca ctatgtaatt
     2521 gaattcaata attcaaaaaa gcaaagtgtt ttataaattc tatttcttta gttttaccta
     2581 aagaaataga atttaatctt aatcattcct aacctatgta ttttccagaa caagataccc
     2641 atttattaaa cgaatctgaa attgacttat taagaattca ttctttagaa accttcggaa
     2701 ctcacgatgg tcccggaatt cgaatggtgg tatttgttca aggttgccaa tttagatgtt
     2761 tgtattgtca aaatccagat tccatagaca ttcacggcgg aacattagta actgtcgatg
     2821 aactggtttc gaaagcagta aaacaaaagg cttattttgg aaaaaaaggt ggagtaaccg
     2881 tttctggtgg cgaacctttg ttgcaaagag ccaaattaat tcacttcttc gaccgtttgc
     2941 acgaaaacgg aatcaacact tgtttggatt cgaatggaag ggttttagac gatcacacca
     3001 aagaactttt agaccgaacc gatttgttgc tcttggatgt aaaacacatc aatcccgaat
     3061 ggcacaaaaa actcactagt ttgaataatg ctactacttt aaaaattgca gaacaccgcg
     3121 aaagtactgg caaaccaatg tggttgcgtt atgtttttgt tcccggttgg accgatcaag
     3181 aagaatattt gcacgaatgg gcgaaacatt tttcgggtta taaaacaatt gaaagagtag
     3241 aaattatccc ttttcatcaa ttgggaaaac acaaatggga gttgatgaat atgcaatatg
     3301 ctcttgcaga aacgccttcg ccaactacag aagcattaga aaatgctaaa gctattttta
     3361 gtcagtattt agataatgtt actataaaat aaattatgac cgtccgctaa gcggactaat
     3421 aactcaaaaa aaacacagat gaaacggatt agacggattt tcgcagatat tatataaaaa
     3481 tccgttttta tcagtttaaa tcagtgtcat ccgtgttcca ttttcaaaat tagtattggt
     3541 tacagatgat cataaaataa ttcaaattac accgcattta agaaattaga ataatgaaaa
     3601 agaataaata tttaatcgtg ttggcaggaa tggttatgca actgtccatc gggtctattt
     3661 atgcctatag taaatggatc gaacctttaa ccacagcatt aaattgggat gctcacgaca
     3721 cgaaaacagg ttttagttta gccatttgct ttctaggatt aaccgctgct tttatgggca
     3781 aatttgcaca aaaagtagga ccgactaaag ctggaataat tgcaacacta tttttaacca
     3841 tcggattatt aggaactgct tatgctgtga gtgttggtaa tatttactta ttttatttaa
     3901 catttggtgt tttgcaagga attggtttag gctttggcta cgtagttcct gtttacacgg
     3961 ttgtaaaatg gtttccagaa aaacctggaa tggcctcagg aattatcatc atgtcttttg
     4021 ctttaggatc gttgttggct tcttttttga ttggcccatt aatcgcttct atgggtttgt
     4081 caggagcctt tgaaacactc gccgttggct atggaatttg tatgttattg agcgctttgt
     4141 atttgtccaa tcctgaaatt acaagcaacg ttcatcacgc tcatatcgat ttgactccca
     4201 aacaaatcat caccgacaaa cgttttatag cgctgtggtt gttgttgttt ctcaatgttt
     4261 gtggtggaat tgccatcatc tccaaagcgg cagttttggg cgaagaagta gcaggaatgt
     4321 ctgcagcaca agctacacta tttgttgcta ttattggttt atttaatgga cttggacgtt
     4381 tgttttggtc tagtatttca gataaaattg gatgttgggc tacttttatg cttttcatcg
     4441 ccataaacgt agtttgtttt gctctgattc ctagtttttc aacaagtttg attgcgtttc
     4501 aagtcttgac ttttattgtt attgctggtt atggagctgg ttttgcaaca atgccatcct
     4561 ttgtaaaaag catttatgga gccgaaaatt acggtcaagt tttgggttat actttaaccg
     4621 cttggtctgc cgctgctttt gttggaccaa tattgttagg attaagcaca gaaatcacca
     4681 tattttattt gttttccata ctattaatca ttgctttgat tgttggaatt tggttaaaag
     4741 gattgttggt aaaaacgact taaaagtaaa aatatattga ttaacaaaaa aagagtctcg
     4801 caaatgatgc gagactcttt ttttgaataa agttaagtaa tataaacaat taaatgttgt
     4861 gttttttgac tttacaactt tcgacttact taataaacta gttcttcttc ttttttaaat
     4921 tcagaaacca attttttctg catctcaaaa gaaaccgatt cataatgatc gaatttagct
     4981 gtaaatctag ctcttccttg agttaaggaa cgtaagtcac taccaaattc ctgcatttct
     5041 gcaaaaggga gaaaagcagt aatcaaagta aattgtcctt cggcatccat actttctaca
     5101 ttggctctgt gggtttgaat attactcata atcgcacctg ttaattcttc tggtgcttga
     5161 attttgatat gataaaatgg ttctaataat tgcggatcag ctttgataaa attttcttta
     5221 aaagccatca ttccagccgt tttaaaggcc aaatcgttgc tgtcaactgg gtgcattttc
     5281 ccatcgtaca aaatcactcg aacatctcta acataagaac ctgtcaatgg gcctttggtc
     5341 attttttcca taattccttt caaaattgaa gggtgaaaac gagtgtcgat agcgccaccc
     5401 acgatgcaat tgtaataaat aagttttccg ccccaaggca aattaatttc ttcttttcca
     5461 cgaatattat agcctttggg ttcatccatt ccttcatgcc aaggttcgat tttcatcgcc
     5521 acttcggcaa attgtcctga acctcccgat tgttttttat ggcgataggt gatttcggca
     5581 cttttttgaa tggtttcgcg atacggaatt tttggacttc gaaattctac cgataattta
     5641 tattgttttt ctaatttcca tttaatgatg gccaaatgta attcgccgtg acaatgaata
     5701 attgtctggt ttaattcagg cgaaacttca attctaatgg ttggatcttc ctctactaat
     5761 tgatgcaaag ccatagataa tttttcttct tccccttttt ttgtgccttc aacagcaata
     5821 gaataagtcg aatttggaaa tacgattgga gtaatttcga tgtgttttcc tttttcgtga
     5881 agtgtgttgt ttatgtgagt gtcttttagt ttcagagtcg ctccaatatc tccagcggtc
     5941 aattcgttta caggagttcg tttgcttcct tctacttcat acaattgatt gatgcgttca
     6001 atttgacctg tattttcgtt agtcaattca tcgcctactt taattttccc tgaaagtacc
     6061 ttaaagaaac ttaaatcacc aatatgaggc tctgagaggt ttttataaat gaatatggca
     6121 gttggagcgt tttcgctgca ttcaatttcc ttaccgtcaa tagttttttg tttgggcatt
     6181 tcataagccg atgggcaaac attgtcaatg aaacccataa ttctaccgct tcccatattt
     6241 ttcttggcag aaacacaaaa aaccgggaaa atttcgtgtt ttatgagcga atgatgcaaa
     6301 cctaatttca tttcatcttc attcaattcg cctttttcga aataaagctc catcaatgtt
     6361 tcgtcatttc cagctatggt ttctattaat tcttgatgca aacgattggc tttttcgatt
     6421 tcattttcag gaattggcag tttttcgggt ttgccaccat tttttggata tttgtacagc
     6481 accatattca aaacatcaac aacagcatca aaattagctc cttgattcaa gggatattga
     6541 accacaacca cgctttgtcc aaaatgatct ttggcttctt gaatggtttt gtcaaagtcc
     6601 gatttttcat tgtctaattg atttacggct aataaaatgg gcaatttgta atcattggca
     6661 tattcccaaa taatatcagt tcccacttcg acacccattg aagaattcaa aagcattaaa
     6721 ccacaatcgg cgacacgcat tgcaccaata atttcaccag caaaatcatc gtatccagga
     6781 gtatctaaaa ggtttatttt gtaacctctc cattttgtat tgactaattt tgagaaaaaa
     6841 gatttacctt tttcttgttc aatttccgaa taatccgcta ctgtattttt ttccagaatg
     6901 gaacctcttc gattgattaa tccagcttca aataacatcg actcaatgag agtcgtcttg
     6961 ccacttccgg tgtgcccaac tagaacaaca tttttaatgt gtttgatatc aaaatttgcc
     7021 atatctcttt gatttaaatt agattagaat atcaaatttc aaaaaaaata attatttcga
     7081 atatgattta tatcatattt tatatagtat aaaaaagcta ttttttggaa gaaaatcttt
     7141 atttgtgaag caccgatttt gagatggtat tggcttcgta tttta
//
LOCUS       DAOSNN010000132         7181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10436_length_7181_cov_5.301572, whole genome shotgun sequence.
ACCESSION   DAOSNN010000132 DAOSNN010000000
VERSION     DAOSNN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10436_length_7181_cov_5.301572"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLQ70_07635"
     CDS             <1..507
                     /locus_tag="PLQ70_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009089594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQE34522.1"
                     /translation="MMGEQTSLVQKEKILSYIKLGKEEGAEILIGGAENHMEGDISGG
                     YYIKPTLFKGNNKMRIFQEEIFGPVLAVTTFKTTEEAIAIANDTMYGLGAGVWTRDAH
                     EIYQVPRAILAGRVWVNQYHSYPAGAPFGGYKQSGIGRENHKMMLGHYRQTKNMLISY
                     DKKKLGFF"
     gene            584..958
                     /locus_tag="PLQ70_07640"
     CDS             584..958
                     /locus_tag="PLQ70_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HQE34523.1"
                     /translation="MIKRIDATEKAVELIKILQEKHGDLMFYQAGGCCEGTQPQCFEK
                     GGFYQRMGDVCIGTIENTEFWVDKDLFEYWKHAHFTLDVIDAFGVGGFSLETPLKKTF
                     RIEYRIFTPEEEQSLEPLIFIE"
     gene            complement(960..1883)
                     /locus_tag="PLQ70_07645"
     CDS             complement(960..1883)
                     /locus_tag="PLQ70_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQE34524.1"
                     /translation="MKLNRALINPPNLSNEKSLKTLVENRTVYSLNHCELNLFETYES
                     SALVPLKFNDLVVTSMLRGKKVMHLFDDPAFEYLPGETVVVPSKVEMKIDFPEAALHN
                     PTQCLALAIDQTKITETLQFLNERYPKEGNDQFWHLNYQNYFFFNNVELATTINKLIK
                     ECLSTSITKDVLADLTLQELLIRIIQTQTAKAIDDGLFTDPKNPITPVIEFIRLNLRE
                     NINMKHLSEKASMSSASFYRLFKRELGMSPIEFIIGEKIRCAKQLLKNPTIQINEVCY
                     LSGFEDSNYFIRLFKKYEGITPKQYQLLYVS"
     gene            complement(1960..2802)
                     /locus_tag="PLQ70_07650"
     CDS             complement(1960..2802)
                     /locus_tag="PLQ70_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008258134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HQE34525.1"
                     /translation="MWRSYIFSFRKSDSFRTLLPFILLIAIYSGGIAYLEVEYLQLDD
                     TSHVKNLSLIHTTLGFVISLLLAYRINSAYDRWWEGRKLWGALVNNSRNLAIKLSVIL
                     KEEKDLAYFRKLIPSYASMLDKHLKDEETSMLLFDDVDLAINHHKHRPNQIAKMLFQK
                     IHTLYEAKKISGDELIILNAEIQSFTDICGACERIKNTPIPYSYSTFIKKFIFLFILT
                     LPYSYVFSLGYYVIAVVPFIFYVLASIELISEEIEEPFGTDANDLPIKKIAENIKKHV
                     EELL"
     gene            complement(2853..4163)
                     /locus_tag="PLQ70_07655"
     CDS             complement(2853..4163)
                     /locus_tag="PLQ70_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE34526.1"
                     /translation="MNTKILFRSLLVFFFCIAKMDAQEVLTIENALKIALENNFEIKM
                     ASNNLTIDKTNVAKGNAGMLPKVTATLVDANSVQNSSQTRQDGTVNALDNAKNNSLNY
                     GVGLDWTVFDGFKMFAKLDQLKELQKLGEAQLKRTIILKISAVNAVYYDLVQQQQQLA
                     ALDTTIVISTQRLTLAQNRFNIGKASKLEVLNAQVDLNTDKVALLRQKESYANRKIAL
                     NQILARDSKIDFKVVDEVTVDDKLVFADLMALAEKQNPQLESEIINKRISELDLKQIK
                     GERYPTLNLTSGYNFAESHSSLGFTSQSSAHGLNYGLRASLNLFDGFAQNRNEKIAKL
                     QIENSKIAIEQQSLLLNSELSTAFQTYLTNLELIQLEEKNEAIAKQNLSITVDKFKIG
                     TITTIEFRAAQLNYINAKVRYSNAQFQAKLSEIALKELAGNMSF"
     gene            complement(4224..>7181)
                     /locus_tag="PLQ70_07660"
     CDS             complement(4224..>7181)
                     /locus_tag="PLQ70_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQE34527.1"
                     /translation="IRTSYTGANADIIESQITEPLEKAINSIDGIRNVTSSSLQGSSN
                     ITVEFNLNKNLEEAANDVRDKVSQAIRNLPQDIDAPPVVSKADANGDAIISMTVQSDS
                     RNPLELSDFAENVIAQRLETIPGVSGVQIWGHKRYAMRLWIDPVKLASYGCTVSEVRT
                     ALNKQNVELPSGKITGNNTELTVKTIGNLSSAEEFNNIIIRTEGDKIVRFSDIGTASL
                     GPENLETKMSQSGMPLVGVAVVPLPGANYLDISKAFYKEFAALKRDLPKDININIALD
                     NTVFVKNSVIEVAETLGISIILVILIIFLFFRDWAIAFRPLIDIPVSLIATFFIMWLF
                     GFSINVLTLLAIVLATGLVVDDGIVVTENIFKKVEEGMTPIEAAIKGSNEIFFAVISI
                     SITLAAVFLPVIFLEGFVGRLFREFGVVIGAAVLISAFVSLTLTPMLNAYLMKGGVQK
                     KSKFYLQTEPFFQKLNSGYANALESFMKKKWLSFPILIVCFGLIYLFFNLLQKETAPY
                     DDRSALTIRTNTAEGSSYEYTERFMQELSRILDDSIPEKKVALVITAPGFLASSVNSG
                     FIRIALKQPDERKLSQKEIAEKLTKITNKYPEAKTSVTEQPTISVNRRGGLPIQYIIQ
                     APNFEKLEEKIPLFMDEVSKSETFSVTDVNLKFNKPEINVSINREKAESLGISVIDIA
                     QTLQLSLSGQRFGYFMRNGKQYQVIGQFEQKDRSKPLDLTSMFVKNNKGELIQMDNVM
                     TIEEQSNPPQLFHNNRYMSATVSAGLAPGKSISDGIEVMNEIKAKVLDDTFTTDLGGE
                     SRDFVESSSNTSFAFGLALLLIFLILSAQFESFIDPFIIILTVPMAVAGALFSLWLFG
                     QTWNIFSQIGTVMLIGLVTKNGILIVEFANQLREQGKTKYDAIIEASEARLRPILMTS
                     LAISLGALPIALSLGAASTSRIGMGVVIVGGTIFSLILTLFVIPALYLMWSKARKHYP
                     EFDHIEEYEKEIK"
BASE COUNT         2246 a         1241 c         1297 g         2397 t
ORIGIN      
        1 atgatggggg agcaaacttc attggttcaa aaagaaaaaa ttctttccta catcaaatta
       61 ggtaaagaag aaggtgctga aattttaatt ggaggtgctg aaaatcatat ggaaggtgat
      121 atttcaggag gttattatat caaaccgaca cttttcaaag ggaataataa aatgcgtatt
      181 tttcaggaag aaatctttgg acctgttttg gctgtgacaa ctttcaaaac aactgaagaa
      241 gctatcgcta ttgcaaatga tacgatgtat ggtttgggag ctggagtttg gactcgtgat
      301 gcgcacgaaa tttaccaggt tccaagagcg attctggctg gtcgtgtttg ggttaaccaa
      361 taccattctt atccggctgg cgcacctttt ggagggtaca aacaatcagg gattggtcgt
      421 gaaaatcaca aaatgatgtt gggacattat cgtcaaacaa aaaatatgtt gatttcctac
      481 gataaaaaga aattaggttt cttttaatag attttatagt tttagctaat tctaagcccg
      541 gcagattgta ttatttgtcg ggttttattt taaattaaaa aagatgataa aaagaattga
      601 tgccacagaa aaagcagtag aattaatcaa aattttacaa gaaaagcacg gtgatttgat
      661 gttttaccaa gctggaggat gttgcgaagg aacgcagcca caatgtttcg aaaaaggagg
      721 attttatcaa agaatgggcg atgtatgcat tggtacaata gaaaacactg aattttgggt
      781 tgacaaggat ttattcgaat attggaaaca cgcccatttt actttggatg tgattgatgc
      841 ttttggagta ggaggatttt ctttggaaac tcctttaaaa aaaacattcc gaattgaata
      901 tcgaattttc acgccagaag aggagcaatc gcttgagcct cttatattta tcgaatagcc
      961 taacttacat ataataattg atattgtttt ggcgtaattc cttcgtattt tttgaacaat
     1021 cgaatgaagt aattggaatc ttcaaatcca gataaatagc aaacttcgtt gatttgtatc
     1081 gtaggatttt tgagtaattg tttagcgcat ctaatttttt cgcctattat aaattctata
     1141 ggactcattc ctaattctct tttgaaaagt cggtagaaag aggcagaact catacttgct
     1201 ttttcgctta aatgcttcat attgatgttt tctctcaaat tcaatcgaat aaattcaatc
     1261 actggtgtaa ttgggttttt cggatctgta aacaatccgt catcaatggc ttttgcggtt
     1321 tgtgtttgta taattcgaat caataattcc tgtaaagtca aatcagcaag aacatctttt
     1381 gtaatcgaag tacttaagca ttcttttatt aatttattga tggtcgttgc caattccaca
     1441 ttattaaaga agaaataatt ttggtaattc aaatgccaaa actggtcgtt accttctttt
     1501 ggatagcgtt cgttaagaaa ctgtaaggtt tcggtaattt ttgtttggtc aatggccaaa
     1561 gccaaacatt gcgtgggatt atggagagcg gcttccggaa aatcgatttt catttctacc
     1621 tttgaaggaa cgacaactgt ttcccctggt aaatactcaa aagccggatc atcaaaaaga
     1681 tgcattactt ttttgcctct cagcatgctg gttaccacca aatcattgaa ttttaaagga
     1741 accaatgcag aagattcgta ggtctcaaaa agattaagtt cacaatgatt taaagagtaa
     1801 acggtcctgt tttccactaa ggtttttaac gatttctcgt tagacaaatt gggtggattt
     1861 attaaagccc ggtttaactt cataagttat tttattaaaa aacagaattt gaaatttaat
     1921 gaaaataact attgaaacaa caaaacttta gaaaaaaaat taaagcaatt cctcaacgtg
     1981 ttttttgatg ttttcggcaa tttttttgat tggtaaatca tttgcgtcag taccaaaagg
     2041 ctcttctatt tcctctgaaa ttaattcaat actggccaat acataaaaaa taaagggcac
     2101 caccgcaatt acataatacc ccaaagaaaa tacataagaa tagggaagtg ttagtataaa
     2161 aaggaaaata aattttttga taaaagtact gtaagaatag gggataggtg tgtttttaat
     2221 acgttcacaa gctccgcaaa tatcggtgaa agattgaatt tctgcattca aaattattag
     2281 ttcatcgcct gatatttttt tggcttcata tagtgtatgg attttttgaa acaacatttt
     2341 tgcgatttga ttgggtctat gtttgtgatg atttattgct aaatctacat catcaaataa
     2401 aagcatactt gtttcttcat ctttcaaatg tttgtctaac atagaagcat agcttggaat
     2461 taatttacgg aagtaagcca aatccttttc ttcttttaat atcactgaaa gttttatggc
     2521 caaattgcgg ctgttgttaa ccaaagcacc ccataatttt cttccttccc accaacgatc
     2581 ataggctgag ttgattcgat aggctaatag caaagagatt acaaaaccta atgttgtgtg
     2641 aatcaaagaa aggtttttaa cgtgactcgt atcatctaat tgaagatatt ctacttctaa
     2701 ataagctata ccgccagaat aaatagcaat taaaagtata aaaggtaata aggttctaaa
     2761 agaatctgat ttgcgaaagc taaagatgta agaacgccat attttcggat tatatgctta
     2821 tcatttagat tttttttttg gacaaaaata aactaaaaac tcatatttcc cgccaattct
     2881 ttcagggcaa tttcagataa ttttgcctga aattgggcat tgctgtagcg cactttggca
     2941 ttgatgtaat tcaattgtgc cgctctaaat tcgatggtgg ttatagttcc aattttaaat
     3001 ttatctacag taatacttaa attttgtttg gcaatagctt cgtttttttc ttccaattga
     3061 atcaattcca agttggtcaa ataggtttga aatgctgttg acaattcaga atttaacaaa
     3121 agactctgtt gttcaatagc aattttggag ttttcaattt gaagtttggc aattttctcg
     3181 ttacggtttt gagcaaaacc atcaaataaa tttaaggaag ctctcaatcc ataattgagt
     3241 ccgtgtgccg aagattgaga ggtaaacccc agactagaat ggctttcagc aaagttgtaa
     3301 cccgaagtca agtttaatgt aggataacgt tcccctttga tttgcttcag atctaattca
     3361 gaaatccttt tgttgataat ttcagattct aattgtggat tttgtttttc ggccaaggcc
     3421 attaaatcag caaatactaa tttatcatcc acagtaactt catcaaccac tttaaaatct
     3481 atttttgaat ctctggccaa aatttgattc aaggcaattt ttctattggc ataagattct
     3541 ttttgacgca aaagggccac tttgtctgtg ttcaaatcta cttgggcatt caagacttcc
     3601 aatttggaag cttttcctat gttaaaccga ttttgtgcca aagtcaagcg ttgggtcgaa
     3661 ataacgatgg tggtgtctaa agctgccaat tgctgttgtt gttggactaa gtcataataa
     3721 actgcattaa cggcactgat ttttagaata atagttcttt ttagctgggc ttctcccaat
     3781 ttttgcaatt ctttcaattg gtctaatttg gcaaacattt taaaaccgtc aaaaaccgtc
     3841 caatccaatc caacaccata attcaagctg ttgtttttgg cattatcaag agcattaact
     3901 gttccgtctt gtcgggtttg cgaactattt tgaacgctgt tggcatcgac caaggtggcg
     3961 gttactttgg gtaacattcc tgcgtttccc ttggctacat tggttttgtc aatcgttaaa
     4021 ttattggaag ccattttgat ttcaaaattg ttttccaagg ctattttcaa ggcgttttcg
     4081 atagttaaaa cttcttgcgc atccattttt gcaatgcaaa agaagaaaac cagtaaactg
     4141 cggaatagaa ttttagtgtt catattttta tttttttgcc atgaatttta gaaaattaaa
     4201 ttcgtgaatt cgtggctaat aaattattta atttcctttt catattcttc gatatgatca
     4261 aattctggat aatgttttcg agcttttgac cacatcaagt acaaagcagg aattacaaac
     4321 aaggttaaaa tcaaggagaa aatagttcca ccaacgataa caactcccat tccaattcta
     4381 ctcgttgatg ctgcaccaag tgacaacgca ataggcaatg ctcccaatga aatggccaaa
     4441 cttgtcatta aaataggtcg aagtcgtgct tccgaagctt ctataatggc atcgtatttt
     4501 gtttttcctt gttcacgcag ttggtttgca aattcgacaa ttaagattcc gtttttggtt
     4561 accaacccaa tgagcattac ggttccgatt tggctaaata tattccaagt ttgaccaaat
     4621 aaccacaatg agaataaggc accagcaaca gccattggca cggttaaaat aataataaat
     4681 ggatcgatga aactttcgaa ttgtgctgat aaaatcaaaa atatcaataa caaagccaat
     4741 ccaaaagcga aagaagtatt cgaactactt tcgacaaaat ccctagattc tccacccaaa
     4801 tcggttgtaa aagtatcgtc cagcactttg gcttttattt cattcattac ttcgattccg
     4861 tcgctaatgc ttttgcccgg agcaagtcct gccgaaacgg ttgccgacat atagcggttg
     4921 ttatgaaaca attgaggcgg attactttgc tcttcgatag tcattacatt gtccatttga
     4981 atcaattcgc ctttgttgtt tttgacaaac atcgaagtta aatccaaggg tttggaacgg
     5041 tctttttgtt caaattgacc aatgacttgg tattgttttc cgtttcgcat aaaataacca
     5101 aaacgttgtc cgcttaacga aagttgcaag gtttgggcaa tgtcaatcac cgatattcct
     5161 aaactttcag ctttctctcg attgatagaa acattgattt cgggtttgtt gaatttgaga
     5221 ttcacatccg taaccgaaaa agtttcactt ttagaaactt cgtccataaa tagaggaatt
     5281 ttttcctcca atttttcgaa attaggagct tgaataatat attgtatagg caatccacca
     5341 cgtctgttta ctgaaattgt tggttgttca gtgaccgatg ttttggcttc gggatatttg
     5401 ttggttattt tagttagttt ttcggctatt tctttttgag aaagttttct ttcatcgggt
     5461 tgtttcaggg caattctgat gaagccgcta ttaaccgatg aagccagaaa tcctggagct
     5521 gtaataacca aagctacttt tttttcagga atagaatcgt ctaaaattct tgaaagttct
     5581 tgcataaacc tttcagtata ttcataactt gaaccttcgg cagtattggt tctaattgtc
     5641 aaagcacttc ggtcatcata cggagcggtt tctttttgta agagattaaa aaacaaataa
     5701 atcagtccaa aacagacgat tagtattggg aagcttagcc attttttttt cataaaactc
     5761 tctaaagcat tggcatagcc actgtttaat ttttgaaaaa aaggttcggt ttgaagataa
     5821 aatttcgatt ttttttgaac gccacctttc atcaaatacg catttagcat tggcgtaagc
     5881 gtaagcgaaa caaaagccga aattaatacg gcagctccta tgacgacgcc aaattctcta
     5941 aacaatctac ctacaaatcc ttcgaggaaa attacaggaa gaaatacggc tgccaaggtg
     6001 atggaaatgg aaattacagc aaaaaagatt tcgtttgatc ctttgatagc ggcttcaatg
     6061 ggagtcattc cttcttccac ttttttgaaa atattttcgg tcaccacaat tccatcatca
     6121 acaaccaatc cagttgccag tacaatggcg agcaaagtca gtacgtttat cgaaaatcca
     6181 aagagccaca ttatgaaaaa tgttgcaatc aatgaaacag gaatgtcaat caatggacga
     6241 aatgcaatag cccaatctct gaaaaacaaa aagataatca agattactaa aatgattgaa
     6301 attcctaaag tttcggctac ttcaatcacc gaatttttta caaaaacggt gttgtcaaga
     6361 gcaatgttta tattgatatc tttgggtaaa tctcttttca aagcagcgaa ttctttataa
     6421 aaagctttcg aaatgtctag ataattagcg cctggaagtg gtacaaccgc aacaccaact
     6481 agtggcattc cagattgact cattttagtt tctaaatttt caggtcctaa actagctgtt
     6541 ccaatatcgc taaagcgaac tattttatcg ccttcagtac gaatgattat attgttgaat
     6601 tcttcggcag aggagaggtt tcctattgtt tttacagtaa gttcggtatt attcccagta
     6661 atttttccag aaggcaattc tacgttttgt ttgtttaaag ctgttctaac ttcagaaacg
     6721 gtacagccgt aggaagctag ttttacggga tcaatccaca aacgcattgc atatctttta
     6781 tgtccccaaa tttgtactcc gcttactcct gggatggttt caagtcgctg ggcgattaca
     6841 ttttcggcaa aatcactcag ttccaatgga tttctggaat cactctgaac ggtcatcgaa
     6901 ataatggcat ccccattggc atcagctttt gaaactacag gtggtgcatc gatatcttgg
     6961 ggtaaattcc gaatggcttg cgaaactttg tcacgaacat cattggcggc ttcttctaaa
     7021 tttttattca aattgaattc aactgtaata ttgctacttc cttgaagact cgatgaagtt
     7081 acattccgaa ttccatcaat cgaattaatg gctttttcta aaggttctgt gatttgggac
     7141 tcaataatat cggcgttggc tcccgtataa ctggttcgaa t
//
LOCUS       DAOSNN010000133         7114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10574_length_7114_cov_6.362941, whole genome shotgun sequence.
ACCESSION   DAOSNN010000133 DAOSNN010000000
VERSION     DAOSNN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10574_length_7114_cov_6.362941"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..1619)
                     /locus_tag="PLQ70_07665"
     CDS             complement(384..1619)
                     /locus_tag="PLQ70_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQE34528.1"
                     /translation="MNLEYFIAKRLITAKHFKSSISAPIIKIAISAIAIGMIMMIVSV
                     ATGIGLQQKIRDKVSAFNGHVIITNYDNNQSEVTLVPISKKQDFYPKFTSVPAVSHVQ
                     AIASKAGIIRTETAFEGIILKGVGTDYQWDNIKEYVVSGKVPDFSKGLNQEVLISQLL
                     ANRLNLKVGDSFNTFFIKENQNKLPNIRRFKISGIFNSGFQDLDATYIIGDIRHIQRM
                     NKWNLNQVGAFEVFANDFDDIKSVGEQVYEKTSSTLDSKTIIEKYSYIFEWLKLFDLN
                     IVIILGVMILVATINMVVALLVLILERTQMIGILKALGSNNWSVRKIFLYNAFYLIIR
                     GLFWGNLIGISLLLIQQYFGIIKLNPENYYVNQAPVYINLGYILLLNLLTVIICFLVL
                     LIPSYIITKISPVKAIRFD"
     gene            complement(1734..2231)
                     /locus_tag="PLQ70_07670"
     CDS             complement(1734..2231)
                     /locus_tag="PLQ70_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQE34529.1"
                     /translation="MLKPNLNELGKHAKDTHNETKKYFDKLKKKAPKNLDYVMQDLHD
                     AEFKKTDCLECANCCKTTGPLFTSADIERISKHLRQKPQQFIDQYLRIDEDKDYVLQS
                     VPCTFLDSDNTCFIYNVRPKACREFPHTDRKKFQQIADLTLKNVAICPAAFNIVEEMK
                     KKLPL"
     gene            2310..3014
                     /locus_tag="PLQ70_07675"
     CDS             2310..3014
                     /locus_tag="PLQ70_07675"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQE34530.1"
                     /translation="MKDLFGKAILDFQTNNSPEDLITETSISEADEMSVAYLFRSYDE
                     MPKLEQKALQLTKGKVLDVGCGAGSHSLTLQNDRNLDVTSIDISPNAIKACELRGLKN
                     ARVQDIMALENEKFDTILLLMNGTGIFGTLKETPNFLQKLKSLLNPNGQILIDSSDII
                     YMFDDDEDGGKWIPSNGYYGELTFSVSYKNETEETFPWLYLDYNTLQNAAFANGLQCE
                     LILEGEHFDYLARLSI"
     gene            complement(3044..4201)
                     /locus_tag="PLQ70_07680"
     CDS             complement(3044..4201)
                     /locus_tag="PLQ70_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQE34531.1"
                     /translation="MKSQFLLDPAITFLNHGSFGACPKPIFGEYQRLQSELENEPVYF
                     ILKKQAEYLKMAKEKLAEYIGCDANDFFFTPNPTFAVNTIMRSLDLKEGDEILSTNHE
                     YGAMDRTWNFYCKKSGAKYIRQNISLPVVSKEQMLEEFWSGYTSKTKIVFLNQISSAT
                     ALIFPVKEICDKARELGLITIIDGAHVLGQMDLNIMELNPDFYTGTLHKWMLAPKGSS
                     FLYVKKDYQPMLDPLVVGWGYESVAPGESQFLDYQEFQGTREISAFLSTPKVIDFLEE
                     NDWKTKSKECRNIVLNNYQRFCDLLNTQPICPITTEFLVQMASVPINTSNPVALKELL
                     FNKYKIEIPVMPLNGNYFIRYSINAYNSQEDLDILYKALEDIIATTDLIEV"
     gene            complement(4256..5008)
                     /locus_tag="PLQ70_07685"
     CDS             complement(4256..5008)
                     /locus_tag="PLQ70_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HQE34532.1"
                     /translation="MKKVFLSIAFVLIAQMTMAQEANFKADVLKLISISGADGAMKVA
                     KLKFLNIIPENKKENFSKEFEASLPSMYEKMAKVYMEIYTPEDIKGMIAFYESPVGKK
                     MSEKSGELTQKAMKAGQEWGKELQVIMEKYKDADSVQDPRNISDNTIYNTAGVEVKPQ
                     FPGGMDQFYKFVAENFKTPKVEKLAGKIYVTFVIEIDGSITGMKVLRDIGYGTGEEAI
                     RVLKLSPKWLPGQQNGKTVRCTYSLPISIGAK"
     gene            complement(5052..5684)
                     /locus_tag="PLQ70_07690"
     CDS             complement(5052..5684)
                     /locus_tag="PLQ70_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HQE34533.1"
                     /translation="MLSKEIQLEVNKGAMLPLMEEFYTIQGEGFHTGTAAYFIRIGGC
                     DVGCHWCDVKESWNAELHPPTSTDLIVANASKYADTVVVTGGEPLTWDMTLLTQKLKE
                     NNRKVHIETSGAYPLSGTWDWICLSPKKNKLPTQTVYDKAHELKVIIYNKHDFIFAEE
                     QAALVNDNAILFLQPEWSKKEEMTPLIVDYVMNNPKWRVSLQTHKYLNIP"
     gene            complement(5794..>7114)
                     /locus_tag="PLQ70_07695"
     CDS             complement(5794..>7114)
                     /locus_tag="PLQ70_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE34534.1"
                     /translation="DTKKKDIEELIAIGRAYTYSTKPNFKKAIEVLNKAKLINPNDAI
                     VQLALGDAYIGDKNQNDAYAAYRNAFQFDPTLLRAKMQLGVLLKGAKSYDEAIKSFNE
                     VIALNPNYGPVYRELAETYYKWGRNKPSKSAEYMQTAIGHYEKYLSLTDHSLHSRMRH
                     ADFLVLVKDYKALEAEANKMIEMDKVNPRIFRYLGYSAYENGNADLAIKSLESFIANP
                     DNKVIAKDYMYLGTAKFKKGLSADGLSIDSNLYNSGLSDIKKSIEIDPASVEELNEIG
                     KKMFTAKLYKEAIPVFELGTTNPEDKNYTEDNIYYGLSIYYSNTKKEVKDIVELQKAD
                     VAFEKVSVASPTYLDAYIYRARTNSMIGNDELTIKFYETYIAKISEKGPEELAKPTTT
                     KKVIESYNNIAASYANTDKAKAIEYLNKTLAIDPTNDYATKSLLKLK"
BASE COUNT         2185 a         1299 c         1180 g         2450 t
ORIGIN      
        1 tttttttcct attcttgcat tttaccctag gaattttgaa gattcaagcg gttttgaaag
       61 gattgggacc cgaaggcact tggttggaat acccattgaa gacgattcgt cgtatcaaaa
      121 taaccggcaa aaacagaact tgactcccca aaatagccca aaaagaagca aacctcttat
      181 tggcacgcag attttgcaga tcctggcaga ttacaaacat atatataagg tagttttttt
      241 ttcgataata ctaaacatat ataaaaagga gaacctatct gccgaaaaca aatgccctag
      301 ccccgatagc agtggaaatc ctcttgtgcc ggggttcggc acaagagatt gcaacggata
      361 gcgggaatag ctccttgaaa aaactaatcg aaacgaatgg cttttactgg agagattttg
      421 gttattatat aggagggaat caataaaact agaaagcaaa ttatgactgt gagaagattt
      481 aaaagtaaga tataccccaa attgatgtaa actggcgctt ggttgacata atagttttcg
      541 ggattgagtt tgattattcc gaagtattgc tgtatgagta gtagagaaat tcctatcagg
      601 ttcccccaga acaatcccct tataattaag taaaaggcgt tgtacaggaa tatttttcgt
      661 acggaccagt tgtttgagcc taaagctttg agtatgccaa tcatttgagt gcgctcaagg
      721 atgagcacta gtaaagcgac taccatattg atagtggcta ccaaaatcat tactcctaat
      781 atgataacga tatttaaatc gaagagtttt agccattcga aaatgtaact gtatttttcg
      841 attatggttt tggaatcgag tgttgatgac gttttttcat aaacctgttc gccaacagac
      901 ttaatgtcgt caaaatcatt tgcaaaaact tcaaaagctc ctacttgatt cagattccat
      961 ttattcattc gttgtatgtg ccgaatatct cctattatat aggtagcgtc gaggtcttga
     1021 aatcctgagt tgaaaattcc tgaaatttta aaccttcgga tgttgggcaa tttattttga
     1081 ttttctttga taaaaaaagt gttgaatgaa tctccaacct ttagattgag cctgttggca
     1141 agcaattgcg aaattaatac ttcttgatta agtccctttg aaaaatcagg cacttttcca
     1201 gaaacaacat attccttgat attgtcccat tgataatctg taccaactcc ttttaaaata
     1261 attccttcga aagctgtttc ggttctaatg attcctgctt tgctggcgat ggcctgaaca
     1321 tgacttactg ctggaactga agtaaatttt ggataaaaat cttgcttctt tgaaatagga
     1381 accaatgtta cttcggattg attgttgtcg taattcgtaa taataacgtg tccgttgaag
     1441 gctgaaactt tgtctcggat tttttgttgc agcccaattc ctgtggctac cgacacaatc
     1501 atcataatca ttccgatggc aatggccgaa atggcaattt ttatgatggg cgcagagata
     1561 ctgcttttaa aatgtttggc agtaatgagt cgtttggcta tgaaatattc tagattcaaa
     1621 atttttagtt tgccacaaag atacataaag ttttttgcca cagattacac ccatttacac
     1681 agatgaaaat agttttatac tttttttcgt tgtgaaaatt tatagcccaa aaattacaaa
     1741 ggaagttttt tcttcatttc ttctacaata ttaaaagcag caggacaaat cgccacattc
     1801 ttcaaagtta aatctgctat ttgctggaac ttctttcggt cggtatgcgg aaattctcga
     1861 catgcttttg gacgaacatt ataaataaaa caggtattgt ctgaatcaag aaaagtacaa
     1921 ggcacggatt gcaatacata atctttgtct tcatcgatac gcaaatattg gtcaataaat
     1981 tgttgtggtt tttggcgcag gtgtttcgaa attcgttcaa tatcggcaga agtgaataat
     2041 ggccctgtgg ttttacagca attggcacat tccaaacaat cggttttctt gaattcggca
     2101 tcgtgcaaat cttgcatcac ataatccaaa ttcttgggcg ctttcttttt taacttgtca
     2161 aaatactttt tggtttcgtt atgtgtatct ttggcatgtt ttccgagttc gtttaaattt
     2221 ggctttaaca ttttttagaa tttcttttgc aaagttaaac aacttttaaa cgcttaaacc
     2281 tctaaacttt taaactttta aactttttta tgaaagacct tttcggaaaa gccatactcg
     2341 attttcaaac caacaattct cccgaagatt taattaccga aacctccatt tccgaagcgg
     2401 acgaaatgag cgttgcgtat ctttttcgaa gctatgacga aatgccaaaa ttggaacaga
     2461 aagcattaca attaaccaaa ggcaaagtac tcgatgtggg ttgcggagcg ggaagccaca
     2521 gtttgacttt gcaaaatgac cgaaatcttg acgtcacttc gatagacatt tcgccaaatg
     2581 ccatcaaagc ttgtgaactt cgtggtttga aaaacgccag agtgcaagat ataatggcgt
     2641 tggaaaacga aaaatttgac accattttat tgttgatgaa cggcacggga atttttggta
     2701 cacttaaaga aacgcctaac tttttacaaa aactaaaaag cctgcttaat ccaaacggtc
     2761 aaatcctcat tgattcctcg gacatcattt atatgtttga cgatgatgaa gacggtggaa
     2821 aatggattcc aagtaatggt tattatggag aactcacatt tagcgtttca tacaaaaacg
     2881 aaaccgaaga aacttttcca tggctttatc ttgattataa tactttacaa aatgcggctt
     2941 tcgccaatgg gttgcaatgc gaattaattt tagaaggaga acatttcgat tatttagcga
     3001 gactttctat atagaaaata gagtatagag aagagaatat agattacact tctatcaaat
     3061 cagtcgtagc aattatatct tccaaagctt tatataaaat atccaagtct tcttgcgaat
     3121 tataggcatt gatggaataa cgtataaaat aatttccatt caaaggcatt actggaattt
     3181 cgattttata tttattgaaa agcaattctt ttaatgctac aggattggaa gtgtttattg
     3241 gaacacttgc catttgtact aaaaactcag ttgtaattgg gcaaatgggt tgtgtattta
     3301 ataaatcaca aaaacgttgg taattattca aaactatatt tctacattcc tttgatttgg
     3361 ttttccaatc gttctcttcc aaaaaatcga tcacttttgg cgtagacaag aatgctgaaa
     3421 tctctctcgt tccctgaaat tcctgataat ccagaaattg actttcgcct ggagcaacac
     3481 tttcgtaacc ccaacctact accaacggat ccaacattgg ttgataatcc ttttttacat
     3541 ataaaaaaga acttcctttt ggcgccaaca tccatttgtg gagcgttcct gtataaaaat
     3601 caggattcaa ttccataatg ttcaaatcca tttgacccaa aacgtgtgct ccgtcaataa
     3661 tggttattaa tcccaattca cgggctttgt cgcaaatttc ctttacagga aaaatcaatg
     3721 cggtggcact cgaaatttga ttaaggaaaa cgattttagt ttttgaagta tagccgctcc
     3781 agaattcttc cagcatttgt tctttggaaa cgacaggaag cgaaatattt tgacgaatat
     3841 atttagctcc cgattttttg caatagaaat tccaggttct gtccatcgcg ccatattcgt
     3901 gattggtgct gagaatttca tctccttctt tcaaatctaa acttcgcata attgtattaa
     3961 ccgcaaaagt tggattgggc gtaaaaaaga aatcattggc atcgcaacca atgtattctg
     4021 ccaatttttc cttggccatt tttaaatatt ccgcttgttt ttttagaatg aaataaaccg
     4081 gttcgttttc caattccgat tgcaaacgtt ggtattcccc aaaaattggt tttggacaag
     4141 caccgaaaga accgtgattt aaaaaagtga ttgcaggatc gagaaggaat tgcgatttca
     4201 ttcggtttag ttttttgtaa atataaaaaa tcccaaacaa tgtttgggaa aattattatt
     4261 tagctcctat agagattggc aaactataag tacatcttac tgttttgcca ttttgttgac
     4321 ctggcaacca ttttggagaa agtttcaaaa cccggatggc ttcttctccc gtaccatatc
     4381 caatatctct caatactttc atcccagtta tactcccatc tatttcaatc acgaaggtaa
     4441 cataaatctt tccagctaat ttttctactt ttggagtttt aaaattttct gcaacaaatt
     4501 tatagaactg gtccataccg cccggaaact gaggctttac ttcaactcca gcagtattat
     4561 aaatggtgtt atcagagata tttcttgggt cttgaacaga atctgcatct ttgtattttt
     4621 ccattattac ttgcaattct ttaccccatt cttgccctgc cttcattgct ttttgagtaa
     4681 gttcacctga tttttcactc atctttttcc cgactggact ttcataaaaa gcgatcattc
     4741 cttttatatc ttcgggcgtg taaatttcca tatatacttt tgccattttt tcatacatac
     4801 ttggcaaaga agcttcaaat tctttcgaga aattttcttt tttattctct ggtataatat
     4861 tcaaaaattt aagttttgca accttcattg ctccatctgc tccactaatc gatatcaatt
     4921 ttaaaacatc tgctttaaaa ttcgcttctt gagccatagt catttgggca attaacacga
     4981 atgcaataga aagaaatact tttttcattt tgttttggtt attagtttat ttcaagaata
     5041 cttaaataaa tttaaggaat attcaagtat ttatgtgttt gtaaagatac acgccatttt
     5101 ggattgttca ttacgtaatc aacaataagt ggagtcattt cttctttttt gctccattcg
     5161 ggttgaagga ataaaatggc attatcattt actaacgccg cttgttcttc ggcaaaaatg
     5221 aagtcgtgtt tgttgtaaat aatcactttt agctcgtgcg ctttgtcgta aacggtttgc
     5281 gtgggcaatt tatttttctt tggcgaaaga caaatccagt cccaagttcc ggaaagcgga
     5341 taagcacccg aagtttcgat atgcactttt cgattatttt cttttaactt ttgagtcaac
     5401 aatgtcatat cccaagtcaa aggttcacca cctgtaacca cgactgtgtc ggcatattta
     5461 ctggcattgg caacaatcaa atccgtgctt gttggcggat gcagttcggc attccagctt
     5521 tctttgacat cgcaccaatg acaacccaca tcgcaaccac caattcgtat aaaataagca
     5581 gccgttcctg tatgaaaacc ttcaccctga atggtataaa attcttccat caagggaagc
     5641 attgcgcctt tgttgacttc taattgtatt tcttttgata acatttcaat aatttaagag
     5701 tgcaaagata gtgaattaaa agcgttgact tataaacata aaaaaaccga tagtcttaaa
     5761 agaactatcg gcttttaaaa gaaaaaatct tgattatttt aatttcaaca atgatttggt
     5821 tgcgtaatca tttgtcggat caatagcaag tgttttattt aaatattcaa ttgccttagc
     5881 tttatcagtg ttagcataac tagccgcaat attattataa gactctataa ctttttttgt
     5941 tgtagttggt tttgctaatt cttcaggtcc tttttcagat atttttgcaa tataagtttc
     6001 ataaaactta attgttaatt cgtcattacc tatcatacta ttggttctag ctctataaat
     6061 ataagcatct agataggtag gagaagctac ggatactttt tcaaaagcga catcagcttt
     6121 ttgtaattca acaatatctt ttacttcttt tttagtatta ctatagtaaa tagacaaacc
     6181 gtagtaaata ttatcctctg tataattttt atcttcaggg tttgttgttc ctaattcaaa
     6241 tacaggaatt gcctctttat acaatttcgc tgtaaacatc ttctttccaa tttcattcaa
     6301 ttcttctaca ctcgcaggat caatttctat tgatttttta atatctgata aacctgaatt
     6361 gtataaatta gaatctattg ataatccatc cgcactcaaa ccctttttga attttgcagt
     6421 acctaaatac atataatctt ttgcaataac tttgttatct ggattagcaa taaagctttc
     6481 caatgattta atagcaagat cagcattgcc attttcgtat gcagaatatc ctaaataacg
     6541 aaagatccta ggatttactt tatccatttc aatcatttta ttggcttctg cttccaatgc
     6601 tttataatct tttacaagta caagaaagtc ggcgtgacgc attcttgaat gcaaagaatg
     6661 atcagtaaga cttaagtatt tttcataatg cccaatagca gtttgcatat actctgctga
     6721 cttcgaaggt ttatttcttc cccatttata ataggtttca gccaattctc tataaactgg
     6781 cccataattt gggttcaaag cgattacttc gttgaaagat tttattgctt catcatatga
     6841 tttagcacct tttaacaaaa cacccaactg cattttggct ctaagcaaag ttggatcaaa
     6901 ttggaaagca tttctataag ctgcataagc atcattttga ttcttatcac caatgtaagc
     6961 atctccaaga gctaattgaa caattgcatc gttaggattt atcaatttag ccttattcaa
     7021 aacctcaata gcttttttaa agttaggctt tgttgaatag gtataagctc ttcctatagc
     7081 tattaattcc tcaatatctt tctttttggt atcc
//
LOCUS       DAOSNN010000134         7063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10692_length_7063_cov_5.172945, whole genome shotgun sequence.
ACCESSION   DAOSNN010000134 DAOSNN010000000
VERSION     DAOSNN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10692_length_7063_cov_5.172945"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PLQ70_07700"
     CDS             complement(<1..666)
                     /locus_tag="PLQ70_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQE34535.1"
                     /translation="MQKITTTALLIVSLFAFSQQKNTVQSKSFSTKNKKITVYTSADS
                     TKLRLTQTDILTFSPSKQPLEIEISVFVEPSKTFQKFMGIGGAITDASAEVFAKLSKD
                     KQKEFMNAYYDKEKGIGYSLLRTTIHSSDFASGSYTYIKEGDKDLKTFNIDHDRQYRI
                     PMIKEAIKTAGGTLLTYASPWSPPAFMKNTNNMLKGGTLLPEYNQSWANYYVKFINSY
                     QKEG"
     gene            complement(698..2125)
                     /locus_tag="PLQ70_07705"
     CDS             complement(698..2125)
                     /locus_tag="PLQ70_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HQE34536.1"
                     /translation="MKSLFLLTVSILFLSCSSSNDVVAQTPTAPVVPNEMDFWLTKAN
                     QSAMLQKQTSVLAFGSTLNNYPNIEVNDAQQFQTIDGFGYTLTGGSAQVINQLNAQKR
                     QELLQELFGTNANSISVSYLRISIGASDLDAVPFTYNDLPDGQTDLNLEKFSLAPNKT
                     DVIPMLKEILAINPNIKIIATPWSPPIWMKDNNSFIGGSLQPKYYGVYANYFVKYIQQ
                     MKAEGITIDAITPQNEPLHPGNNPSLLMPATDQADFIKNHLGPIFQNANLKTKIIVYD
                     HNCNKPEYPISILKDAAAYPYIDGSAFHLYEGDISTLSTVHNAFPNKNIYFTEQYTSN
                     TGNFDGDLKWHTRNIIIGSMRNWSKTALEWNLANNASFGPNTPGGCTTCKGALTIESS
                     TNFTRNVAYYIIAHASKFVPAGSVRIASNLSGNLQNVAFKTPGGKKVLIVENDGTTSE
                     SFNIKHNGKWILTSLEAGSVGTYIW"
     gene            complement(2387..3838)
                     /locus_tag="PLQ70_07710"
     CDS             complement(2387..3838)
                     /locus_tag="PLQ70_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQE34537.1"
                     /translation="MKLFKNYLKIMTALMLLFLTSCSDYLDTEPITEEATSLNDGIVI
                     KDAAAAESRMNAVYGTFGSGYWQLDFFLVGDGQSDNAYMGADNPAIFQYETYQMLSTN
                     SQMKGDWNDIYNNINTCNIVINYVDQATDLSATRKNEMIGEASLIRALNHFQAVQLWG
                     DCPIAKEAVFSISSANFEEAFNELFPVRKPVAEVYAQIIADCQVAIQKAPDASYKFKA
                     NKMAANALLAKVYATMPNPDWTKVNQYCDVVIGGGYTLLPTFDHLFDSAHEGNAESIW
                     EVNGDGWGSPIGAWNTFMFLGTDWKKFNAPSHTLIQAYTNNGDTQRLATTVTRENVGW
                     SDAYWSSTGYPFAYKMRDTNGNQNNYLARLADILLLKAEALASTGDFNGAMVQVNKVR
                     ARAGIAPISASSQSIAIDKVLEERLMELAFEGDRWFDLKRMGKAIQMLSQQKDGSGNV
                     LPFATNINQNRLLWPIPQDKLDANALLTQNPGY"
     gene            complement(3866..6856)
                     /locus_tag="PLQ70_07715"
     CDS             complement(3866..6856)
                     /locus_tag="PLQ70_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE34538.1"
                     /translation="MKLTKLFIFCLSSLLFSIYVQAQEVNVKGKVNDENGMAIPGVTI
                     LIKGTSKGTTSDADGNYQIKAASSGTLVFSYLGYTSVKEPINGRTSINVKLHTESQSL
                     QEVVINVGYGTQKMKNSTNAVSKVKSDAFEDRPIVSVAQAIQGNAAGVNVVQTSGKPG
                     ASFDVRIRGLSSINSENSPLYVVDGIQTKDISGLNTEDIVDLSILKDATATAIYGVNG
                     STGVVIITTKKGKSNKNDFQFSSYLGLSKSVSNVDVLNLTDYKSLITEINPSYLTTAN
                     DAKYTGINTNWQDEILKTGIDQNYNLSYAGGTEKVKFYGALGYQMVDGVINPSKYNRF
                     SGKINVNASLTPWLKTDVNMNIIQSDGSYISDNNSVGQGGAVMSALVTPAFLPTWGSQ
                     LNVRETNADGSYKDGYKEGQYAENPYQSGWENPVSLLSRLNTTAVNRYLSSISFDVNI
                     VHNLVWKPMLSLDWTKSDNVQFVDPYTNVWGRNGDNNPDSDKGRGSNTVENNKNWNFE
                     NTLNYVLKFDNSDLNLLGGMSMQKNNYTKDANSGTGFPVEQTSYDINAAKNQSFENRE
                     NEVRSLSYFGRAIYTIKNKYILNGVFRESGASQLSKDNKWGFFPGVSAAWIISNENFL
                     KDVTTISELKLRGGWGKTGNLSGLPAYSSFDLNYTNPLTNVTTLDQIGNPDLKWETTT
                     DINLGLDLGLFKNRIKFTADIYKKNTKDLLQIIYFPGFSKPYYYNAGEIENKGLELSL
                     NTINLTGDFKWNTNFNISFNDNQVVKLGLLKKLPFQNLTSVGESVILLTEGSALGSFY
                     GYKVDKVDPTTGTLLYKDINGDGNITPDDRTNIGNPNPNFTFGLSNDFSYKAFSLDVL
                     ITGSEGGDIFNASRMDLTLMNNYNNQSTEVLNRWTTPGQITDVPKANDASALHVSDRF
                     VEDGSYVRLKAVTLGYQFKLEKWRLNSLKLYVTGQNLYTITNYKGFDPEVGAFNNATG
                     IGQGIDLGTYPQVKTFVFGLKANF"
BASE COUNT         2158 a         1279 c         1136 g         2490 t
ORIGIN      
        1 tccttctttt tgataactat tgataaattt cacataataa ttggcccaag attgattgta
       61 ttctggaagc aatgtccctc ctttaagcat attgttcgtg tttttcataa aggcaggcgg
      121 actccacggt gatgcataag tcagcaatgt tcctccagcg gttttaatgg cttccttaat
      181 cattggaatg cgatattgcc tgtcgtgatc gatattgaaa gtttttaaat ctttgtctcc
      241 ttccttgata taggtatagc ttccgctggc aaaatcagaa ctgtgaatcg tagttcttag
      301 taaggaatat ccaatccctt tttctttgtc gtaataggca ttcataaatt ctttttgctt
      361 gtctttggat aatttggcaa atacctcggc gctggcatcg gttattgctc ctccgattcc
      421 cataaatttc tgaaaagttt tagagggttc tacaaacaca gaaatttcaa tttccagggg
      481 ttgttttgac ggggaaaaag ttaaaatatc ggtttgtgtc aatctcaatt ttgtgctgtc
      541 agccgaagtg taaaccgtaa tttttttgtt ttttgtagaa aaggatttag actgaactgt
      601 gtttttttgc tgagaaaaag caaaaagaga tactatcaaa agtgctgttg ttgttatttt
      661 ttgcatacca ttatattcta taggtttatt tttttattta ccaaatgtaa gttcccaccg
      721 aacccgcttc taatgatgtt agaatccatt ttccgttgtg tttaatattg aatgattctg
      781 aagtggttcc gtcgttttcc acaattaaaa ctttttttcc acccggtgtt ttgaaagcaa
      841 cattttgtaa atttccactt aggttacttg caattctaac tgaacctgct ggtacaaatt
      901 tggatgcgtg agcaataata taataggcca catttctggt aaaattagta ctgctttcga
      961 tagttaaggc tcctttacaa gtagtacaac caccaggagt atttggacca aaagaagcgt
     1021 tattggccaa gttccattct aatgctgttt tgctccaatt gcgcattgag ccaataataa
     1081 tatttcttgt atgccatttt aaatctccat caaagttgcc tgtattcgag gtgtattgct
     1141 ctgtaaaata gatgttttta ttcgggaatg cgttatgaac ggttgacaat gtactaatat
     1201 ctccttcata caaatgaaag gccgatccgt ctatgtaggg ataggcagca gcatccttta
     1261 aaattgaaat agggtattca ggtttattgc aattatggtc atagacaatg attttagttt
     1321 tcagattggc gttttgaaaa atcggtccca aatgattttt aataaaatcg gcttggtctg
     1381 tagcgggcat taacaaactt ggattatttc ctggatggag cggttcgttt tggggagtta
     1441 tggcatcaat agtaattcct tcggctttca tttgttggat gtatttcaca aaatagttgg
     1501 cataaacgcc ataatatttg ggttgcaaac ttccgcctat aaaactgttg ttgtctttca
     1561 tccaaatggg tggagaccaa ggagtggcaa ttattttgat attgggattg atggccagaa
     1621 tttctttcaa cataggaatc acatcagtct tatttggagc caaactaaat ttttcgagat
     1681 tcaagtctgt ttgtccatct ggcaaatcgt tataagtgaa aggtactgcg tccaaatcgg
     1741 aagcaccaat actaatcctg agataactta cggaaatcga attggcattt gtaccaaaca
     1801 attcttgcaa taattcctgt cttttttgtg cattcaactg atttataact tgagcacttc
     1861 cgccagttaa ggtataacca aatccgtcaa tggtttgaaa ttgttgagca tcattgacct
     1921 ctatattagg ataattatta agagtcgagc caaaagctaa aactgaagtt tgcttttgaa
     1981 gcattgcgct ttgattggct tttgttaacc aaaaatccat ttcatttgga accacaggag
     2041 cagttggagt ttgagcaaca acatcattgg acgatgaaca actcaaaaaa agaatggata
     2101 ccgttaacag aaataaactt ttcatatgtt gatatttatt gtttttatcg gtcatatgta
     2161 attcgcatat ctttatcagt gtttactacc gatttttagg aatgctcatt tcatagtttt
     2221 atgtgtaata atttgtgttt taattccata caacttcaat attaatcggt aaaaacaatc
     2281 cgattactat tttaaacttt ttaaatataa ataaatccat caataagaac gaaaattatt
     2341 ttcataacat ttgaaactgt tcttattgat ggattaaaaa acaacactaa taaccaggat
     2401 tttgtgtcaa gagtgcatta gcatctaatt tatcttgagg aataggccat aaaagtctat
     2461 tttgattaat attagttgca aatggcagca catttccact accatctttt tgttgagata
     2521 acatttgaat tgcttttccc attcttttta aatcaaacca acgatcacct tcgaaagcca
     2581 attccattaa tctttcttcc agaactttat caattgcaat agattggctt gaagcactaa
     2641 ttggcgcaat tccggctctg gctcttacct tatttacttg gaccatcgct ccattaaaat
     2701 ctcctgtact tgccaaagct tcggctttta gtaataaaat atcagccaaa cgagcaagat
     2761 agttgttttg attgccattg gtatctctca ttttataagc aaaaggatag cctgttgaag
     2821 accaataagc atcagaccaa ccaacatttt ctctcgttac agtcgttgcc aatctttgag
     2881 tgtcgccatt attggtataa gcttgaatta gagtatggga aggagcattg aattttttcc
     2941 aatcggttcc taaaaacata aaagtattcc aagcaccaat tgggcttccc catccgtctc
     3001 cattaacttc ccaaatggat tcagcatttc cttcgtgtgc gctatcaaaa agatgatcaa
     3061 aagtgggcaa aagtgtataa ccaccgccaa taaccacgtc gcaatattga tttactttgg
     3121 tccaatctgg attgggcatt gtggcatata cttttgcaag aagtgcattg gccgccattt
     3181 tgttggcttt aaatttataa gaagcgtcag gtgctttttg gatagccact tggcaatcgg
     3241 caatgatttg tgcataaact tcggcaactg gttttcttac agggaaaagc tcgttaaatg
     3301 cttcttcaaa gttagcgctt gaaattgaga aaacagcttc ttttgcaata gggcaatctc
     3361 cccataattg aacggcttga aaatgattta gcgctctaat caatgaagcc tcaccaatca
     3421 tttcgttttt tcgggtggca cttaaatcgg ttgcttgatc aacataatta atcacaatat
     3481 tacaagtgtt aatgttgttg taaatatcat tccaatcgcc tttcatttgc gaattagtcg
     3541 atagcatttg ataggtttca tattgaaaaa tggcagggtt atcagcgccc atataagcat
     3601 tatcagattg tccatcacca accaagaaga aatccaattg ccaataaccc gagccaaaag
     3661 ttccatacac agcattcatt ctagattcgg cagcagcggc atcttttatt acaataccat
     3721 cgttaagtga agttgcttct tcagtaattg gttcagtgtc taaataatcg gaacaggatg
     3781 ttaaaaacaa cagcattaat gctgtcatta tttttaaata atttttaaat aatttcataa
     3841 ctatatcgta tttatatttt tagtattaaa aattggcttt tagtccaaaa acaaacgttt
     3901 taacttgagg ataggttcct aaatcgatac cttgaccaat tcctgtagca ttattaaaag
     3961 ctccaacttc agggtcaaat cctttgtaat tcgttattgt atataggttt tgtccagtta
     4021 catataattt taatgagttc aatctccatt tttccaattt aaattgatat ccaagagtaa
     4081 cggctttcaa tcgaacataa gaaccgtctt ctacaaatct atcagaaacg tgtaaagcac
     4141 ttgcatcatt ggcttttgga acatcagtaa tttgacccgg ggtagtccat ctattcaata
     4201 cttcggtcga ttgattgttg taattattca tcaatgttaa atccattctt gaagcattga
     4261 agatatctcc accttctgaa cccgtgatta aaacgtctaa actaaatgct ttataactaa
     4321 aatcattgct taaaccaaag gtgaaattag gattaggatt tcctatatta gttctgtcat
     4381 ctggtgtgat atttccatcg ccatttatgt ctttgtacaa caatgtacct gttgtaggat
     4441 ctactttgtc cactttatat ccatagaagc ttcctaatgc acttccttcg gttaatagaa
     4501 ttacactttc tccaactgaa gttaaatttt ggaatggtaa ttttttaagc aaccctaatt
     4561 taaccacttg attgtcattg aaggaaatat tgaaattagt attccatttg aaatcgccag
     4621 ttaagtttat tgtatttaaa cttaattcca atcctttgtt ttcaatttca ccagcattgt
     4681 aataataggg ttttgaaaaa cctgggaaat aaataatttg aagcaagtct ttagtattct
     4741 ttttatagat atccgctgtg aatttgattc tgtttttgaa taaacccaaa tctaaaccta
     4801 gattgatatc agttgttgtt tcccatttca aatctgggtt tcctatttga tctaaagtgg
     4861 taacattcgt caacggatta gtataattca aatcaaagct tgaataggct ggcaatccag
     4921 ataaattacc tgtttttccc caaccacctc ttaattttaa ttcactaata gtagttacat
     4981 ctttcaagaa attttcattc gaaataatcc aagcggcaga aactcctgga aaaaatcccc
     5041 atttattgtc tttggataat tgagaggcac cactttctct aaatacgcca ttcaaaatat
     5101 atttattttt aatagtgtag atggctcttc caaaataaga cagacttctt acctcatttt
     5161 ctctgttttc gaatgattga tttttagcag cattaatatc ataactagtt tgctctacag
     5221 gaaatccggt tccagaattg gcatctttag tataattgtt tttctgcata gacatacctc
     5281 ctaataagtt taaatctgag ttatcaaatt tcaacacata gttcaaagtg ttttcaaaat
     5341 tccaattctt attgttttct accgtattac tacctcttcc tttgtcggaa tcagggttgt
     5401 tatccccatt tcttccccaa acattggtgt atggatctac aaactgaacg ttatctgatt
     5461 ttgtccaatc taatgaaagc attggtttcc aaaccaaatt atgcacgata ttgacatcaa
     5521 aactaatgct gcttaagtat ctatttacag ctgttgtgtt aagtcttgac aataatgaaa
     5581 ctgggttttc ccaaccagat tgatatggat tttctgcata ttgaccctct ttatatccat
     5641 ctttataaga tccatctgca ttagtttctc taacatttaa ttgagatccc caagtaggca
     5701 aaaatgcagg cgttactaaa gcactcataa ccgcaccacc ttgtccaaca ctattattat
     5761 cactgatgta agagccgtca gattgaataa tattcatatt tacatctgtt tttaaccaag
     5821 gagtaagact agcattcaca tttatttttc cagagaaacg gttgtatttt gatggattta
     5881 ttacaccatc taccatttgg tagcctaaag cgccatagaa ttttactttt tcggtacctc
     5941 ctgcataaga caaattataa ttttgatcta taccagtttt caaaatttca tcttgccaat
     6001 tggtatttat tccagtgtat tttgcatcgt ttgcggtggt caaataactt ggattaattt
     6061 cagtaatcaa tgatttataa tctgttaaat ttaaaacatc tacattgcta actgattttg
     6121 acaaacctaa ataagaacta aattggaaat catttttatt cgattttcct tttttagtcg
     6181 taataattac aactccagtt gaaccattaa caccatagat agctgttgca gtggcatctt
     6241 ttaaaataga taaatccaca atatcttcgg tattcaaacc agaaatgtct ttagtctgga
     6301 ttccatcgac aacatataag ggtgagtttt cagaatttat ggaactaagc cctctaattc
     6361 tcacatcaaa acttgcgcct ggttttcctg aagtttgcac cacatttacc ccagcagcat
     6421 ttccttgtat tgcctgagca accgatacaa ttggacgatc ctcaaacgca tccgacttga
     6481 ctttagaaac tgcatttgtt gaatttttca tcttctgagt tccataacct acattaatta
     6541 caacttcttg caacgattgt gactcagtat gaagttttac attaatacta gttcggccgt
     6601 taatgggttc cttaacggaa gtatagccca aataactaaa tactaaagta ccgctagagg
     6661 cagcctttat ttgataattc ccatccgcat cagaagtagt tccctttgag gttcctttaa
     6721 ttaaaattgt tactccggga atggccattc cattttcatc attcactttt ccttttacgt
     6781 tcacctcttg tgcttgaacg taaattgaaa ataagagaga tgaaagacaa aaaataaata
     6841 attttgttaa tttcattttt ctaaaaattt tggttaatta attagtaatt tcatccaaaa
     6901 gtacagctgt ttttttgtta agtgtaataa ttattcatta caaaaaccat acaaagttca
     6961 tttttgaact ggacaaaaac tatacaaaca ttttttaatt atttaaatgt caatgtttta
     7021 aaaattaatt tatgttataa agaaaaacat ttatgtcaaa aaa
//
LOCUS       DAOSNN010000135         7055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10711_length_7055_cov_4.207286, whole genome shotgun sequence.
ACCESSION   DAOSNN010000135 DAOSNN010000000
VERSION     DAOSNN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10711_length_7055_cov_4.207286"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLQ70_07720"
     CDS             complement(<1..375)
                     /locus_tag="PLQ70_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQE34539.1"
                     /translation="MRIIKYLFLLLLLSLVALSIFIATQKGVFTVERSKIINSQKSSL
                     FNYANDLKNWKEWNSLAVEDSLLNIVYSNNTIGKGSFCSWDGKEGAGELKTINVKQDD
                     SILQTMDINGNIADISMSFKDTL"
     gene            651..2687
                     /locus_tag="PLQ70_07725"
     CDS             651..2687
                     /locus_tag="PLQ70_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQE34540.1"
                     /translation="MKLLKIAAVFMTVGAGLVCNAQTAKTKDVKIAAEVSFDYVAEQF
                     ADIKVLRYQIPGWENLTLKEQKLVYYLTQAGYSGRDIGWDQHYKYNLKIRKALENIYV
                     NYKGDKTSNDWKNFEIYLKRVWFANGIHHHYSNDKIKPSFSAAYFNGLLKATKTSLSP
                     AIVDVLFNNADAKKVNLDESKGLLEGSAINFYDKGITAKEVADFYAKKTSPDPKRPYS
                     FGLNSKLVRNSKGQLEEKVWKSGGMYAAAIDKIIYWLTKAQTVAENKQQGDAIGLLIS
                     YYKTGSLKTWDDYNIAWSQATEGNIDYINSFIEVYNDPLGYRGSYESIVQIKDFDMSK
                     KMEVISKNAQWFEDNSPLLLENKKKNVVGVSYKTVVVAGESGDSSPATPIGVNLPNSD
                     WIRAEHGSKSVSLGNIIDAYNKAGGKGRLQEFANDPEEIALAEKYAEFGDKMHTALHE
                     VIGHASGQINPGVGTPKETLKSYASTLEEGRADLVGLYYVYSPKLQELGLVDDWKKLG
                     MESYDSYIRNGLMMQLIRLEPGADIEEAHMRNRQYISAWVFEKGKKDNVIEKITRNGK
                     TYFNITDYDKLHELFGQLLREIQRIKSEGDYEAGKALVENYGVKVDQAIHAEVLERNK
                     QFTSAPYSGFVNPILVPKMDAKGEIISIEVKQPNSFAEQMLYYSKNYGFLPLEN"
     gene            complement(2746..2934)
                     /locus_tag="PLQ70_07730"
     CDS             complement(2746..2934)
                     /locus_tag="PLQ70_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34541.1"
                     /translation="MFSQGQMMFAGFFVIIFIIAMIYAYRKDAKLHKLFYQGNFKILI
                     GFALFIVLLFVIKIFFKR"
     gene            3015..3398
                     /locus_tag="PLQ70_07735"
     CDS             3015..3398
                     /locus_tag="PLQ70_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HQE34542.1"
                     /translation="MQKQINAVKEFHTAFKIGHSDIPRADLGETKNTLRYNLMKEENE
                     EYLEAVQNNDLIEIADALGDMMYILCGTIIEHGLQHKIEEVFDEIQRSNMSKLGEDGK
                     PIYREDGKVMKGPNYFKPDFSKILK"
     gene            complement(3524..4591)
                     /locus_tag="PLQ70_07740"
     CDS             complement(3524..4591)
                     /locus_tag="PLQ70_07740"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HQE34543.1"
                     /translation="MSATQTNKIEIIKAATSKIQDVDFDNLHFGEVFTDHLFECDFKN
                     GEWQKPIIKPYAPFLMDPSSKVFHYGQAIFEGMKAYKDDNNDIWLFRPDENFKRFNNS
                     ATRMAMPEVPEEIFMEGLKQLLQLDEAWVQKGNGSSMYIRPFMIATGAGVMANPSDEY
                     KFMILLSPAKAYYSGEVKVLIAEHFSRAANGGIGAAKAAGNYAAQFYPTNLANKEGFQ
                     QVIWTDDATHTKLEEAGTMNVFFRINNTLLTAPTSERILDGITRKSLIEMAKSEGINV
                     DVRPVLVSELVEAAKNGSLKEIFGAGTAAVVSVIKGFSYQDVYYEMTPIENSYASQLK
                     EKLTNLQNKISEDAFGWTVKV"
     gene            4865..5527
                     /gene="mnmD"
                     /locus_tag="PLQ70_07745"
     CDS             4865..5527
                     /gene="mnmD"
                     /locus_tag="PLQ70_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HQE34544.1"
                     /translation="MKRNIIQTRDGSTTIHIEEWDECYHSRFGAIQEAQHVFIKKGLS
                     LFENESVSILEIGFGTGLNAFITFLEFQKFNQKINYVAVEGYPVALEEASLMNYVAEL
                     NAENNRPIFEKLHECNWEEPHVLSEDFIFTKRKQFFADINDFEKFDLIYFDAFGFDVQ
                     PELWTATIFKKMYDALKKQGVLVTYAARGVIKRNMIESGFRVEKHEGAPGKREMFRAI
                     KE"
     gene            5665..5862
                     /locus_tag="PLQ70_07750"
     CDS             5665..5862
                     /locus_tag="PLQ70_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34545.1"
                     /translation="MTKVMFDYTKSVLERVSFDATLFCKELEKAIKSLLPYEMELLRE
                     WLLNFIIEKPELKQCLLIVND"
     gene            complement(5893..6834)
                     /locus_tag="PLQ70_07755"
     CDS             complement(5893..6834)
                     /locus_tag="PLQ70_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQE34546.1"
                     /translation="MTITQLQYVLAVAEHKNFTLAADKCFVTQPTLSMQIQKIEEELN
                     ILIFDRTKKPIQLTAIGQKIVEQAKNIVNEAGKIKDIVEYQKGFIGGEFRIGIIPTIM
                     PTLLPMFLNNFIKKYPKIKLIIEELNTNEIITRLKNGHLDAAIAATPLEDEKIKEIVL
                     YYEPFVAYVPTNNVISQKSEIEISDLNLDEILLLQDGHCFRDGILNLCKNQQMAGKNS
                     FQLESGSFETLIKLADEGLGTTLLPYLHTLNLNDNDQLKLRQFKEPKPAREVSLIYPK
                     SELKIHIIDALRNTISGVVKGAIAFQNVQIISPISKK"
     gene            6941..>7055
                     /locus_tag="PLQ70_07760"
     CDS             6941..>7055
                     /locus_tag="PLQ70_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HQE34547.1"
                     /translation="MKLNTIGLPTKEAEVLAAELNVLLANFQIYYQNLRGLH"
BASE COUNT         2464 a         1058 c         1184 g         2349 t
ORIGIN      
        1 caaagtatct ttgaaactca tagaaatatc agctatattt ccattaatat ccatagtttg
       61 aagaatacta tcgtcttgtt ttacgtttat cgttttcaat tcaccagctc cttcttttcc
      121 atcccaagag caaaaactgc cttttccaat tgtattattc gaatagacaa tatttaaaag
      181 tgaatcctca acagccaatg aattccattc tttccaattt tttaaatcgt tggcataatt
      241 gaatagggat gatttttgcg aattaataat tttgcttctt tcaacagtaa atactccttt
      301 ctgagtggca ataaaaatgg ataatgctac taaactaagt aataataaaa gaaagaggta
      361 ctttataatt ctcataaatc taggggttaa tttaagttgt aaagttagaa atattattca
      421 aacattttac ttcacgacaa agtataaaaa aattctcgga taaatataaa acataaatgg
      481 tatttttaat tattaatttc aaaaataatc gattaatact actaaaacag gaaggtagag
      541 tcaaatttgc actagctaat atgtgattct ttgtatattt atttgttata aattatctaa
      601 ataaatgtac atttgttaaa tattttttta aaactactaa attattaaac atgaaattac
      661 ttaaaattgc tgccgttttt atgaccgttg gagctggttt ggtttgtaat gcacaaacag
      721 caaaaactaa ggatgtaaaa attgcagcag aagtttcttt tgattatgtc gccgaacaat
      781 ttgccgatat aaaagtgttg cgttatcaaa ttccaggttg ggaaaactta accttgaaag
      841 agcaaaaatt agtttattac ctaacacaag caggttattc tggacgtgat attggttggg
      901 atcaacatta caaatacaat ctaaaaatta gaaaagcact cgagaatata tatgtaaatt
      961 acaaaggtga taaaacttca aatgattgga aaaattttga aatttaccta aagagagttt
     1021 ggtttgctaa cggaattcat catcattatt caaatgataa aattaaacca agtttttctg
     1081 ctgcctattt taatggttta ttgaaagcta caaaaacaag tttatctcca gcaattgtag
     1141 atgttctttt taacaatgcg gatgctaaaa aagtaaatct tgacgaatct aaaggattgc
     1201 ttgaaggttc ggctatcaat ttttatgaca aaggaattac tgccaaagaa gtggccgatt
     1261 tttatgcaaa gaaaacaagt ccagacccca aaagacccta ttcttttggt ttgaattcaa
     1321 aattagttcg aaattcgaaa ggacaattag aagagaaagt ttggaaaagt ggtggaatgt
     1381 atgctgctgc catcgacaaa atcatttatt ggttaacaaa agcacaaact gttgctgaaa
     1441 ataaacaaca aggcgatgcc attggtttac ttatcagtta ctacaaaaca ggaagtttaa
     1501 aaacttggga cgattacaac attgcttggt cgcaagctac cgaaggaaat attgattata
     1561 taaatagttt tattgaagta tataatgatc ctttgggata tcgaggttca tacgaaagca
     1621 tcgttcaaat caaagatttt gatatgtcta aaaagatgga ggtaatttcg aaaaatgctc
     1681 aatggtttga ggataattcg cctttgcttc tagaaaataa aaagaaaaat gtggttgggg
     1741 tttcttataa aactgttgtt gtagctggtg aatctggcga ttcttctcct gcaacaccaa
     1801 ttggtgtaaa ccttccaaac tccgattgga ttcgtgccga acatggttcc aaatcagttt
     1861 cattaggaaa tattattgac gcttataata aagctggtgg aaaaggaaga ttacaagaat
     1921 ttgcaaacga tccagaagaa attgctttgg ccgaaaagta tgctgaattt ggggataaaa
     1981 tgcataccgc tttgcacgaa gtgattggtc acgcctcagg acaaataaat cctggtgtag
     2041 gaacacctaa agaaaccttg aaaagttatg cttcgactct agaagaaggt cgagccgatt
     2101 tagtgggatt gtattatgta tatagcccaa aattgcaaga actaggtttg gttgacgatt
     2161 ggaaaaaact tggaatggaa tcttatgata gttacattcg aaatggtttg atgatgcaac
     2221 ttattcgttt agaaccagga gccgatatcg aagaagccca tatgagaaac cgtcaataca
     2281 tcagtgcttg ggtattcgaa aaagggaaaa aagacaatgt gattgagaaa attactcgca
     2341 acggaaaaac ctatttcaac attacggatt atgataaatt acacgaatta tttggacaat
     2401 tattgcgtga aattcaacgt atcaaatctg aaggtgatta tgaagcagga aaagctttgg
     2461 ttgaaaatta cggtgtaaaa gtagatcaag ccatacatgc cgaagttttg gaaagaaaca
     2521 aacaatttac ttctgcacct tatagtggtt ttgtgaatcc aattttagtt ccaaaaatgg
     2581 atgctaaagg agaaattatt tcgattgaag taaaacaacc caattccttt gcagaacaaa
     2641 tgttgtatta ttctaaaaac tatggtttct tgcctttaga aaattaataa ataattgaaa
     2701 tataaagaaa gggatgtcgt aattggcatc cttttttatt tagttttatc ttttaaaaaa
     2761 tatttttatc acaaacaaaa gcacaataaa tagggcaaaa ccaatcaaaa ttttgaagtt
     2821 tccttggtag aataatttat gcaacttagc atcttttcga taagcgtaaa tcattgcgat
     2881 tataaaaata ataacaaaaa aaccagcgaa catcatctga ccttgactaa acatagtata
     2941 attttttttc gcaaatttag ggttttgttt aggaaaactt gctaatttgc caactcattt
     3001 aatcaaataa aattatgcaa aaacaaatca acgcagtcaa agagtttcat acggctttca
     3061 aaattggtca tagtgacatt ccaagagccg atttgggaga aaccaaaaac acgcttcgct
     3121 acaatttgat gaaagaagaa aacgaagaat atcttgaagc ggttcagaat aatgatttaa
     3181 tagaaattgc cgacgctttg ggcgatatga tgtatattct ttgcggaact attatagaac
     3241 acggtttgca acataaaatt gaagaagttt tcgacgaaat tcaacgttct aatatgagta
     3301 aattaggcga agacggaaaa cctatttatc gcgaagacgg aaaagtgatg aaaggcccga
     3361 attattttaa acctgatttt tctaaaattc tgaaatagtg ttcagtattt agtctcagtt
     3421 tgcagtacat taaaaaagag cgattttcaa ttagaaaatt gctctttttt taattgtaat
     3481 ttagttatct aataacagaa cactaataac tgaaaactgt caactatact ttcacagtcc
     3541 aaccaaaagc atcttccgaa attttatttt gaagattggt tagtttttct tttaattgag
     3601 aagcgtatga attttcaata ggtgtcattt cataataaac gtcttggtaa gagaatcctt
     3661 tgataacact tacaactgca gcagttccag caccgaagat ttcttttaaa gatccgtttt
     3721 tagcagcttc aactaattca gaaactaaaa ctggacgaac atcaacgtta ataccttcac
     3781 tttttgccat ttcaataaga ctttttcggg taataccatc caaaattctt tcgcttgtag
     3841 gagcagtcaa taaagtgtta tttattctaa aaaacacatt catcgttccg gcctcttcca
     3901 gtttggtatg tgtggcatcg tcagtccaaa tcacttgttg aaaaccttct ttattggcta
     3961 aattggttgg ataaaactgt gcagcataat tacctgcggc ttttgcagca ccaattccac
     4021 cgtttgcagc tctactgaaa tgctcggcaa tcaatacttt tacttcgcca gaatagtaag
     4081 cttttgcagg agacaaaaga atcataaatt tatattcatc cgatgggttt gccatcactc
     4141 cagctcctgt tgcaatcata aacggacgga tatacatact gcttccgttt cctttttgaa
     4201 cccaagcttc atctaattgt agcaattgtt taagtccttc cataaagatt tcttcaggaa
     4261 cttcaggcat agccattctt gtggcagaat tgttaaaacg tttaaagttt tcatcaggtc
     4321 taaacaacca aatgtcgtta ttatcatctt tatatgcttt catcccttca aaaatagctt
     4381 gtccataatg aaatactttt gaagaaggat ccatcaaaaa tggggcataa ggcttgataa
     4441 ttggtttttg ccattcacca tttttgaaat cacattcaaa taaatgatcg gtgaaaactt
     4501 ctccaaaatg taaattatca aaatctacat cttgtatttt tgaagttgca gcttttatga
     4561 tttcaatctt gttggtttgt gttgcgctca taattggtta ttctatttaa ttttaataaa
     4621 aacagatgat tctatgcgaa tactctgttt gcaaaattaa acaaaaatca tcaaattact
     4681 tatcaaaaaa atagtattta tgacatttaa ctgagattta tttatattga aaaaaaaatg
     4741 gtaaatgtgg agttgaattt atggaatatc caacaaattc gaagcattaa tggttataca
     4801 taaattggat ttaattaaat ttggcttttt taaacccaat ttaaaaccaa aaatcaattt
     4861 tattttgaaa agaaatataa ttcaaactcg agatggctct actaccatac acattgaaga
     4921 atgggatgaa tgttatcact ctcgatttgg agccattcaa gaagcacaac atgttttcat
     4981 aaaaaaagga ctttctttgt tcgaaaatga gtcggtttct attttagaaa ttggatttgg
     5041 gacgggcttg aatgctttta tcactttttt ggaatttcaa aaattcaatc agaaaattaa
     5101 ttatgtggca gtcgaagggt atcctgtcgc tttagaagaa gcttccttaa tgaattatgt
     5161 tgccgaatta aatgccgaaa ataatagacc tatcttcgaa aaactacacg aatgcaattg
     5221 ggaagaacct catgttttaa gtgaagattt tattttcaca aaaagaaagc agttttttgc
     5281 agatattaat gatttcgaaa aatttgattt gatttatttt gatgcttttg gatttgatgt
     5341 tcaacccgaa ttgtggacag ctacaatttt caaaaaaatg tatgatgccc ttaaaaaaca
     5401 aggtgtttta gttacttatg ctgcccgagg agtgatcaaa cgaaatatga ttgaatcagg
     5461 ttttagagtc gaaaaacacg aaggagcacc tggaaaaaga gaaatgttta gggcaataaa
     5521 ggaatagatg gagctaaaca tttctctttt tcaacacaat aaaagcacat tataattcgt
     5581 tgttaaattt agttttatta taaaaataat agcttacttt tacaacattt agaacagaga
     5641 tctaacccta aatcttgaaa aattatgact aaagtaatgt ttgattacac taaatcagtg
     5701 ctagaaagag tgagttttga tgccacactt ttttgtaaag agttagaaaa agcaataaag
     5761 tctttgttac cctacgaaat ggaacttttg cgagaatggt tattaaattt cataatcgaa
     5821 aaaccagaat taaaacaatg tttgttaatt gtaaacgatt aaaatgataa ggctgtcaaa
     5881 agacagcctt ttttatttct ttgaaatagg actaattatt tgaacatttt gaaacgcaat
     5941 tgctccttta accacgcccg aaatagtgtt tctaagcgca tcaataatat ggattttcaa
     6001 ctcgctttta ggataaatta ggcttacttc acgagcaggc ttgggttctt tgaattgtct
     6061 tagttttaat tggtcgttat cattcaaatt taatgtatgt aagtagggca ggagcgtggt
     6121 gcctaatcct tcgtcggcta gttttattaa agtttcaaaa ctaccgcttt caagttggaa
     6181 tgaattttta ccggccattt gctgattctt gcataaattt aaaattccat ctctaaaaca
     6241 atgtccatct tgtaataata aaatttcgtc aaggtttaaa tccgatattt cgatttctga
     6301 tttctgagaa atgacattat tcgtagggac ataagctaca aaaggctcat aatacagcac
     6361 aatttccttg attttttcgt cttctaatgg agtggctgca atggccgcat cgagatgacc
     6421 attttttaat cgcgtaatga tttcattggt attcaactct tcgataatga gttttatttt
     6481 gggatatttt ttgatgaaat tattcaaaaa cattggtaaa agtgtaggca taatggttgg
     6541 aataattccg atgcgaaatt caccaccaat aaatcctttt tggtattcga ctatatcctt
     6601 tatttttcct gcttcgttaa cgatattttt ggcttgttca acaattttct gaccaattgc
     6661 tgtaagttga ataggttttt tggttctatc aaatatcaaa atattcaatt cttcctctat
     6721 tttttgaatt tgcatgctca atgtgggttg ggtaacgaaa catttatcgg ctgcaagagt
     6781 gaaattttta tgttcggcta ccgccaatac atattgtagt tgagtaattg tcattgtata
     6841 gtattttacg atgcaaatat aaaaacaatc aatttaattt atagtattaa gaacggttat
     6901 ttcttaattt tgattaaaat taaatataaa agacaacatt atgaaattaa atactattgg
     6961 attaccaact aaagaagctg aagtattagc tgcggaatta aatgtattat tggccaattt
     7021 tcaaatttat taccagaatt taagaggttt gcatt
//
LOCUS       DAOSNN010000136         6969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10887_length_6969_cov_5.535001, whole genome shotgun sequence.
ACCESSION   DAOSNN010000136 DAOSNN010000000
VERSION     DAOSNN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10887_length_6969_cov_5.535001"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1094
                     /locus_tag="PLQ70_07765"
     CDS             60..1094
                     /locus_tag="PLQ70_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQE34548.1"
                     /translation="MKKKVTLKQIAKELDVSISTVSKSLRNSLEIGEETRLKVQAFAK
                     FYHYKPNNIALSLKNRKTKTIGIIIPEIIHYFFSTVINGIEHVANENGYSVIICLSDD
                     SFDKEVLNMEMLANGSIDGFIMSLSKETQFKSDFHHITEVINQGMPVVMFDRVTNDIL
                     CDKVIINDELAAYEAVQNLIDKGRKKIALVTTVDYVSVGKFRTDGYIKALRDNGLPFD
                     ENLIIKIENVENCEITIAQLLEDKAIDAVFAVNELFAVTIIKIANKIGIKVPEDLAVI
                     AFTDGIISKYSTPTITTVTQNGVEMGNVAAKILIERLEAEEDESEDEQEEIYKTVVIE
                     THLIERESTL"
     gene            1403..2734
                     /locus_tag="PLQ70_07770"
     CDS             1403..2734
                     /locus_tag="PLQ70_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34549.1"
                     /translation="MKKPKLSFWQIWNLSFGFLGVQIGYSLQNGNTSRILEALGADVH
                     SIGYFWLAAPLAGLIVQPIIGLSSDKTWTRLGRRIPFIFFGAIVSALAMFFMPNAEYF
                     TYLLPPLIFGAVMLLLMDTSFNVTMQPFRALVGDMVNDEQKNLGYSLQSALINFGAVF
                     GSLLPWILAKAGIANVPAAGEKVAASVIWSFYIGGTILLITVLWTVFRTKEYGPKEHA
                     QYNDIDLEAEHVTEKTSILKLISNAPKIFWQLGIVQFFSWFALFLMWVYTTRAIANQV
                     WGPEALDPKSIGFNEAGDWTGVLFAFYSAVAALFSLLIPTIAKSIGRKKTYSFSLLMG
                     GIGLASMYFVHDKTILLLSISGVGLAWAAILAMPYAMLSGSLPADKMGVYMGLFNATI
                     TIPQIAAGLLGSTIIALFGGFPMAIIVIAGISMLIAGLAVFFVKESVSTAK"
     gene            2747..3400
                     /gene="pgmB"
                     /locus_tag="PLQ70_07775"
     CDS             2747..3400
                     /gene="pgmB"
                     /locus_tag="PLQ70_07775"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HQE34550.1"
                     /translation="MNTKAFIFDLDGVIVDTAKYHFLAWKKIADSLGFEFTHQNNELL
                     KGVSRVRSLDIILELGKVKASQEDKNRWLIQKNEDYLSYLVDMDESEILPGVMPILNY
                     LKEKKQLIALGSASKNARPILEKTGTLSYFDAIVDGNDVSNAKPDPEVFLLAAKLLNT
                     KPEDSIVFEDSVAGVQAANIGGMTSVGIGEAATLHEAKYLFKDFTQMDKTFIDNLIK"
     gene            3473..5767
                     /locus_tag="PLQ70_07780"
     CDS             3473..5767
                     /locus_tag="PLQ70_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HQE34551.1"
                     /translation="MNQDYIKADNWSIIEEGFDAESVKSSESLFSIGNGAMGQRANFE
                     ENYTGETFQGSYIAGIYYPDKTKVGWWKNGYPKYFAKVLNAPNWIGIDIEINDEILDL
                     NTCTQVKNFRRELNMKEGWYNRSFEATLKNGTEIGVNIRRFLSMNLDEVGIINYEITA
                     LNKDTKIVYKPYVDAGVTNEDANWEEKFWEPLDVKRTGNAAFVTAQTFKTHFKVTTFM
                     QNSIFVNDKNGNISPSNIKTGTDKIQFSYDVIIAKGQKSSIQKIGGYTVSMNHENTVT
                     AAETVIKEALAKGYDQLLADQIEAWSKIWEMSDITIEGDVKAQQGIRFNIFQLNQTYS
                     GKDSRLNIGPKGFTGEKYGGSTYWDTEAYCIPFYMATKDQQVARNLLTYRYNQLDKAI
                     ENAKDNLGFTNGAALYPMVTMNGEECHNEWEITHEEIHRNGAIAFAIFNYHRFTGDYS
                     YIPEKGLEVLIGIARFWHQRANFSTDKNQYVILGVTGPNEYENNVNNNFYTNYIAKWC
                     IDYAHEQILKVSLEYPSDHKRIIEKVKLSDAELQDWKKVSGNMYFPYSEKHGVYLQQD
                     GFLDKDLVQVKDLDKSQRPINQKWSWDRVLRSPYIKQADTLQGFYFFEDHFTTEQLEK
                     HFDFYEPFTVHESSLSPCVHSIQAAKLDKMDLAYTFYLRTSRLDLDDYNKEVEEGCHI
                     TSMAGTWMSIVEGFGGMRVKNDALHFSPKIPKEWTGYTFKINFRNQILKVEINHNETK
                     FSLEGTQELTVFVNEDAVLVKPNL"
     gene            5883..6969
                     /locus_tag="PLQ70_07785"
                     /pseudo
     CDS             5883..6969
                     /locus_tag="PLQ70_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213301.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT         2445 a         1270 c         1231 g         2023 t
ORIGIN      
        1 ccattatcca aataatattt tatctttatt acgaaaatga tttttatcaa attacaagca
       61 tgaaaaagaa agtaacccta aagcaaattg caaaagaact ggatgtatcc atttctaccg
      121 tttcaaaatc cctaagaaac agcctcgaaa taggagaaga aaccagattg aaagttcagg
      181 cttttgccaa attttatcac tataaaccca acaatattgc acttagttta aaaaatagaa
      241 aaacaaaaac catcgggatt atcattcccg aaattataca ttatttcttc tcgactgtca
      301 tcaacggaat cgaacatgta gctaatgaaa acgggtatag tgtaataatc tgtttgtctg
      361 atgattcatt cgacaaagaa gtactaaata tggaaatgct tgccaatggt agcatcgacg
      421 gattcattat gtcgctctcc aaagaaaccc aattcaaaag cgattttcac cacattaccg
      481 aagttatcaa tcaaggaatg ccagtggtga tgtttgatag agttacgaat gatattcttt
      541 gtgataaagt aattatcaac gacgaattag ctgcttacga agccgttcaa aatttgattg
      601 acaaaggccg aaaaaaaatc gcccttgtaa ccacagtaga ttatgttagc gttggaaaat
      661 ttagaaccga cggttacatc aaagcactta gagacaacgg attacctttt gacgaaaatt
      721 taattatcaa aattgaaaat gtcgaaaatt gcgaaattac tatcgcccaa ttactagaag
      781 acaaagccat cgatgccgtt tttgctgtaa atgaattatt tgccgtaacc atcatcaaaa
      841 tagccaataa aataggaata aaagttcccg aagacttagc cgttatcgca tttaccgatg
      901 gaattatttc aaaatattca acgccaacta ttacaacagt aacccaaaac ggagtagaaa
      961 tggggaatgt agcagcaaaa attctaatcg aaagattgga agccgaggaa gatgaatcag
     1021 aagatgagca agaagaaata tacaaaactg tagttatcga aacccatttg atagaacgcg
     1081 aatcaactct ttaaaaaaat caatggcaat aacaatatca atattcaagt aatttaaaat
     1141 tgctattgtt attgccattg aaattgccat tgaaattgcc attgttatta ttttttcacc
     1201 actcccacta gccttctaac taaaacttat tcacactatt acgttgtagt ttttgaaata
     1261 aatacttttc taacctctaa cttctaactt ctaacttctt ttaatatatt tacaacattc
     1321 aaaactttta cttttacttc gaaaaacaag ttaagttttg ataagcaagg tgcttatcgt
     1381 acatttaaaa caatatacga taatgaaaaa acccaaatta agtttttggc aaatctggaa
     1441 tctaagcttt ggattcctag gagtacaaat tggatattct ctccaaaatg gcaataccag
     1501 ccggatttta gaagcgctcg gagccgatgt tcacagtatt ggatattttt ggctcgcagc
     1561 accacttgct ggtttgattg ttcagccaat tattggtctt tctagtgata aaacctggac
     1621 tcgtcttgga cgcagaattc catttatatt ttttggagcc attgtttcgg ctttggcaat
     1681 gttctttatg cccaatgcag aatactttac ttatttgtta ccgcctttaa tttttggcgc
     1741 tgtaatgcta ctgttaatgg acacttcctt taatgtaacc atgcaacctt ttagagctct
     1801 cgttggcgat atggtcaacg acgaacaaaa aaatctgggc tattctttgc aaagtgcttt
     1861 aatcaatttt ggagctgttt ttggttcctt attaccttgg attctggcca aagcaggaat
     1921 tgcaaacgtg cccgcagctg gcgaaaaagt agcagcatct gtaatctggt ctttttatat
     1981 tggtggaaca attctattga taactgtgct ttggacggtt tttagaacca aagaatacgg
     2041 acctaaagaa catgctcaat acaacgacat cgacttagaa gctgagcatg taaccgaaaa
     2101 aacaagcatt ctcaaattaa tttcaaacgc cccaaaaata ttttggcaat taggaatcgt
     2161 acaattcttt tcttggtttg ctttattttt aatgtgggtc tataccacca gagccattgc
     2221 taaccaagtt tggggaccag aagctttaga cccaaaatcc attggtttca acgaagcagg
     2281 agattggaca ggagtattat ttgcctttta cagtgcggtt gcagctttat tctcattact
     2341 aatcccaaca attgccaaat caataggaag aaagaaaacc tatagctttt cacttttaat
     2401 gggcggaatc ggcttggctt cgatgtattt tgtacacgac aaaaccattt tattactttc
     2461 gatttctgga gtaggattgg cttgggcagc tattttagcc atgccttacg ctatgctttc
     2521 aggttcgcta ccagccgaca aaatgggagt ttatatggga ttattcaatg ccaccattac
     2581 cattccacaa attgctgcag gactattagg aagcacaatt attgctttgt ttggaggttt
     2641 tccaatggca attattgtaa tcgcaggaat ttcaatgttg attgctggtc tagccgtatt
     2701 ttttgtcaaa gaaagcgttt caacagctaa ataaataatt ataataatga acacaaaagc
     2761 atttatattc gaccttgacg gcgtaattgt ggacaccgca aaatatcatt ttctagcatg
     2821 gaaaaaaatc gccgattctc taggtttcga attcacacac caaaacaacg aattactaaa
     2881 aggagttagc cgtgtgcgct cgcttgatat aattttagaa ttaggaaaag taaaagcttc
     2941 acaagaagac aaaaacagat ggctcattca aaaaaatgaa gattatttgt cctatttagt
     3001 cgatatggac gaaagcgaaa tcctaccagg agtgatgcct attttaaact atttgaaaga
     3061 aaaaaaacaa cttatagcct taggctctgc cagtaaaaac gctagaccta ttctcgaaaa
     3121 aacagggact ttatcttatt tcgatgctat tgttgacgga aatgacgtgt cgaatgccaa
     3181 acccgatccc gaagttttct tgcttgctgc caaattgtta aacactaaac cagaagattc
     3241 aattgttttt gaagactcag ttgcaggagt tcaagcagcc aatattggcg gaatgacaag
     3301 cgtgggaatt ggtgaagcag caaccctgca cgaagcaaaa tatttattca aagattttac
     3361 ccaaatggat aaaaccttta tcgataattt gattaaataa atttgtttca agtttcaagt
     3421 ttcaggtcaa agaacttgaa acttgaaacc tgaaaccttt aaactaaaaa aaatgaacca
     3481 agattatata aaagcagaca attggtccat cattgaagag ggatttgatg cagagagcgt
     3541 aaaatcgtcc gaaagtcttt tcagcattgg caacggtgcg atggggcaac gcgcgaattt
     3601 tgaagaaaat tacacagggg aaacctttca aggaagttat atagcaggaa tttattatcc
     3661 tgataagacc aaagtaggtt ggtggaaaaa tggctatcca aaatattttg ctaaagtatt
     3721 aaacgctcca aattggattg gaattgatat cgaaatcaat gatgaaattc tagatttaaa
     3781 cacttgcact caagtaaaaa actttcgtcg tgagttgaat atgaaagaag gttggtacaa
     3841 tcgctctttt gaagctactt tgaaaaacgg aaccgaaatt ggtgtaaata ttcgtcgttt
     3901 tctaagtatg aatctggacg aagtgggaat cattaactat gaaatcaccg ccttaaataa
     3961 agatacaaaa atagtttaca aaccttatgt tgatgctggt gtaaccaatg aagatgccaa
     4021 ctgggaagaa aaattctggg aaccattgga tgtaaaaaga accggaaatg ctgcatttgt
     4081 aaccgctcaa accttcaaaa cgcatttcaa ggttacaact tttatgcaaa atagcatttt
     4141 tgtaaacgac aaaaacggca acatttctcc ttccaatata aaaacaggaa ccgataaaat
     4201 tcaattttca tacgatgtga ttattgccaa aggacaaaaa tcgtccattc aaaaaattgg
     4261 tggttacacg gtttctatga atcacgaaaa cactgttact gccgcagaaa ctgtaataaa
     4321 agaagcttta gcaaaaggat atgaccaatt attagctgac caaatcgaag cttggtccaa
     4381 aatttgggaa atgtcagaca tcaccatcga aggcgatgtg aaagcacaac aaggcattcg
     4441 ttttaatatt tttcaattga atcaaaccta ttctggcaaa gattctcgtt tgaatattgg
     4501 tccaaaagga tttactggcg aaaaatacgg aggctcgact tattgggata ccgaagccta
     4561 ctgtattcca ttctatatgg ctacaaaaga ccaacaagtc gccagaaatt tattgacata
     4621 ccgatacaat caattggaca aagccattga aaatgctaaa gacaatttgg gttttacaaa
     4681 cggagccgct ttgtatccaa tggtgaccat gaatggcgaa gaatgtcata acgaatggga
     4741 aatcactcac gaagaaattc atagaaatgg agcgattgct tttgcgattt tcaattatca
     4801 tcgttttact ggcgattaca gctatattcc cgaaaaaggg ttggaagttt taatcggaat
     4861 tgcccgtttt tggcatcaaa gagcgaattt ttcgacagac aaaaatcagt atgtaattct
     4921 aggcgttacg ggtccaaacg aatatgaaaa caatgtaaac aataattttt acactaatta
     4981 tattgccaaa tggtgtattg attatgcaca cgaacaaatc ctgaaagtat cattagaata
     5041 tccatcagac cataaacgca tcatcgaaaa agtaaaatta tcggatgctg aattgcaaga
     5101 ttggaaaaaa gtttcgggca atatgtactt tccatattcc gaaaaacacg gagtttattt
     5161 acaacaagac ggctttttag ataaagattt agtacaagta aaagatttag ataagtcgca
     5221 acgccccatc aaccaaaaat ggtcttggga tagagttttg cgttcgcctt acatcaaaca
     5281 agccgatact ttacaagggt tttacttctt tgaagatcat tttacaacgg aacaattaga
     5341 gaaacatttc gatttttacg aaccgtttac cgttcacgaa agttcgcttt cgccttgcgt
     5401 gcattccatt caagcggcaa aattagataa gatggattta gcctatacat tttatctaag
     5461 aacctcacgt ttagaccttg acgattataa caaagaagta gaagaaggct gccacatcac
     5521 ttcgatggcg ggaacttgga tgagtattgt agaaggtttt ggtggaatga gagtcaaaaa
     5581 tgatgcgctt catttctctc caaaaattcc aaaagaatgg actggatata cctttaaaat
     5641 caatttcaga aaccagattt tgaaagtgga aatcaatcac aatgaaacca aattctcgct
     5701 cgaaggaaca caagaactga ctgtatttgt caatgaagat gccgttttgg tgaaaccaaa
     5761 tctataaata acaatataaa ataaacatat aaggcattta agttcattta agtttctctt
     5821 gaattttctt atttgcctta tcggttttga aaaaggttga aataaattta attatttgtc
     5881 aaatgaaaaa actattttta ttgttcttaa tttttacgac ctcaactatg ttcgcacaaa
     5941 tcgaaagaat agaacctcct ttttggtatg ccgggatgaa aaatcccgaa ttacaaatca
     6001 tgttttatgg caaaaacatt gcgaaatata atgtttctac caccaattct attcccatta
     6061 caaaagtgga gcggacatca aatcaaaatt acattttcgt taccattaat accaaaaata
     6121 cacctccaaa agagtttcat ttccttttta aagaaaaaga taaagttgtt tttactgaaa
     6181 aatacaactt aaaacagcga agagaaaact cagccgaacg caaaagtttt gacgcttcag
     6241 atatgatgta tttgatcatg ccagatcgat tttctaacgg caattctaat aacaacagcg
     6301 attcctaacc accgaaaaat acaaccgaga attacccgaa ggacgacacg gaggcgacat
     6361 tcaaggcatt atcaatcatt tagattacat caaagaactc ggcgcaacaa ccctttggat
     6421 tacaccactt tgcgaagaca acgaagccgt atattcctat catacctacg cccaaaccga
     6481 tgtttacaaa attgaccccc gctacggaac caacgaggat tatgccagat tagcttctga
     6541 aatgcataaa aaaaacttaa aattagtgat ggattatgtt accaatcatt ggggaatgca
     6601 acattggatg atgaaggatt tacctactca agattggatt catcaatttg aaaatttcac
     6661 acaaaccaat catagaagaa cagtaattca cgatataaac gcatcaaaaa gcgataccaa
     6721 aatatgcatg gatggatggt ttgcgccatc gatgcccgat ttgaatcaaa ccaatccgtt
     6781 ggtattaaat taccttactc aaaacgccat ttggtggatt gaatttgcca acttagacgg
     6841 tttcagagtc gatacttaca attatgccga tccaactgcc atcacaaaat ggaccaaagc
     6901 catcaccgac gaatatccaa attttaatat cgttggcgaa atttcgatgc gcgatcaagc
     6961 tcaactttc
//
LOCUS       DAOSNN010000137         6962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10905_length_6962_cov_6.730853, whole genome shotgun sequence.
ACCESSION   DAOSNN010000137 DAOSNN010000000
VERSION     DAOSNN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10905_length_6962_cov_6.730853"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PLQ70_07790"
     CDS             complement(<1..1021)
                     /locus_tag="PLQ70_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HQE34552.1"
                     /translation="MKKIIYKIAVLLLLILSSGISYAQQIAFPGAEGYGKYTVGGRGG
                     VVYEVTNLNDSGTGSLRAAVEASGPRTVVFRVSGTITLNSDLKISNPYLTIAGQTAPG
                     DGICLRKYALVFNNAVHDVIVRYLRVRLGNESGGESDAVSARDCSNIILDHVSASWSV
                     DETMSLYFCPNITIQWCMVSESLYNSNHPKGNHGYGGINGSNNSTSHHNLLAHHSSRN
                     PRMASGCGYFDYRNNVIYNWGFNSTYGGEQQQVGDVNHAFSTINMVANYYKPGPATKP
                     GNITYRIVNPSSRNLADDYGKWYVDANVMVGNTAVTANNWNGGVQPQDGNAYLAGLKL
                     DNPWPA"
     gene            complement(1137..2129)
                     /locus_tag="PLQ70_07795"
     CDS             complement(1137..2129)
                     /locus_tag="PLQ70_07795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQE34553.1"
                     /translation="MGDVAMTVPVVRAFVNQYPLVKITVVSRPFFKPFFEGISNVSFF
                     AFDEKQRHKGFFGLLRLFQDLKGLQIDAFADLHNVLRSKVVRILFFLSGKKVAFVDKG
                     RAEKAALTRAENKIFKPLTTMFERHVKVFEQLGFKVELSHPTFPEKAILDSEIFSLIG
                     ENNQKLIGIAPFAQYDSKVYPLDLMQEVINELAEEKNHKILLFGGGKKEIELLDSLSK
                     GKENIVVVAGKLKFQEELQLISNLDVMLSMDSGNAHIAAMLGVKVITLWGATHPFAGF
                     SPFNQPLENALVSDRNLFPKLPTSVYGNKKVEGYEEAMRTISQEQVVSKIKSQL"
     gene            complement(2264..2869)
                     /locus_tag="PLQ70_07800"
     CDS             complement(2264..2869)
                     /locus_tag="PLQ70_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HQE34554.1"
                     /translation="MFSKLAYSVFEQSIQDYHQFDNVDQPINNPFPKEKIEHLLYLKN
                     WIDTVQWHFEDIIRDPNIDPVAALTLKRRIDASNQERTDMVEYIDGYFLQKYAHIAVK
                     NDAKINSESPAWAFDRLSILALKIYHMQEEATRAEASQDHRDKCQAKLNILLEQRTDL
                     STAIEELLTDIENGDKFMKVYKQMKMYNDDDLNPVLYQNKK"
     gene            2995..3648
                     /gene="upp"
                     /locus_tag="PLQ70_07805"
     CDS             2995..3648
                     /gene="upp"
                     /locus_tag="PLQ70_07805"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQE34555.1"
                     /translation="MQTHYLSEDNSILNHFLGQIRNVNVQHDSMRFRRNIERIGEVMA
                     YELSKDLQYRNIEIQTPLGIKKTTEIADKLVLCSILRAGLPLHLGFLNYFDSAENGFV
                     SAYRHHPNNDEFFDILVEYQAVPNIENKTLLLIDPMLATGQSMVAVFNKLMEKGLPKE
                     IHIAVIIGAPEGIAYLEKHLPDSCHLWVATLDEKLNDKKYIIPGLGDAGDLAYGNKL"
     gene            complement(3643..4572)
                     /locus_tag="PLQ70_07810"
     CDS             complement(3643..4572)
                     /locus_tag="PLQ70_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HQE34556.1"
                     /translation="MITSVFKKSTPINLILVVILMLVFFFIYQFQDLSWTKSVVSILK
                     TFGLFLVLLASVFITNFIAKRNGLSKDSGYTLFFYFLFLLFFPSVWNNTNLIASNFFI
                     LLALRRLISLQSLKATKEKIFDASLWIFVASLFHFWSILYIILVFISVIFHVARDYRN
                     WVLPFIAFFAVGIISVGISLLCNKDLLGYISQNAVFNFNIDYFTNNYQNIAFSIYITV
                     ALFFVVSMFASLSSKPLLLNSSFKKIIMAFFIGLLIFVISSNKSNDMLIYTIAPLSIM
                     ATSHIEVKQLQLKQELVLGVLILCSLFAFFSQL"
     gene            4621..4851
                     /locus_tag="PLQ70_07815"
     CDS             4621..4851
                     /locus_tag="PLQ70_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQE34557.1"
                     /translation="MKAFFEGIQWLFETIFFALHDFLRELELTHWFAANLLNWIFMAI
                     CSVAIVYWCKQLKIYDEKGEENQDTTAHSFLK"
     gene            complement(4887..6161)
                     /gene="purD"
                     /locus_tag="PLQ70_07820"
     CDS             complement(4887..6161)
                     /gene="purD"
                     /locus_tag="PLQ70_07820"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQE34558.1"
                     /translation="MNILLLGSGGREHAFAWKMVQSPLCDTLFVAPGNAGTAQIAKNI
                     DISATDFDAIKALVIKENIEMVVVGPEDPLVKGIYDFFKNDDDLKHIPVIGPSKLGAT
                     LEGSKEFAKEFLMKHKIPTAAYDSFTAETVEKGCEFLATLQPPYVLKADGLAAGKGVL
                     IIQDLAEAQEELRNMLVGQKFGAASSKVVIEEFLDGIELSCFVLTDGKSYKILPTAKD
                     YKRIGEGDTGLNTGGMGAVSPVPYVDAVLMEKIETRIVKPTIEGFQKDGIEYKGFVFI
                     GLINVKNEPIVIEYNVRMGDPETEVVIPRMKSDLVELFLAVANEKLDEFELEIDERSA
                     TTVVVVSGGYPEDFEKGKVISGFENVENSIVFHAGTKLNNGNIVSNGGRVLTVTSYGD
                     NFEEAIKKSYQNIDKLHFDKMYFRKDIGNDLK"
     gene            6290..>6962
                     /locus_tag="PLQ70_07825"
     CDS             6290..>6962
                     /locus_tag="PLQ70_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HQE34559.1"
                     /translation="MFPLFDFSLLINGFPMKEAKAELQKIQSIPEEEFETFIENKKQE
                     IVDYHLKNNSSYQNLVGKTTFNTWKDLPIMTKKDFQKPLIERLSTEFSLKKVYVNKTS
                     GSSGDPFIFAKDKFSHALTWANIINRFGWYGIDFNSSYQARFYGIPLDFIGNKKERIK
                     DFFTNRFRFPIFDLSDKVLDRFLQQFKIKRFDYINGYTSSIVLFAKFLQQKNIVLKNI
                     CPTLKV"
BASE COUNT         2317 a         1314 c         1065 g         2266 t
ORIGIN      
        1 ttgctggcca aggattatcc aattttaatc cagcaagata agcattgccg tcttgaggct
       61 ggacgccacc gttccaatta ttggcagtta cagcagtatt acctaccata acatttgcat
      121 ctacatacca ttttccgtaa tcgtcagcca aattacgtga agaaggattg acaatgcgat
      181 aagtaatatt gcctggtttg gtcgcaggtc ctggtttata ataattagcc accatattta
      241 ttgtcgaaaa agcatgattg acatccccaa cttgctgttg ctcgcctcca taagtactat
      301 tgaaccccca attgtatatt acattatttc tataatcaaa atacccacaa cctgaggcca
      361 ttcgaggatt tcgactagaa tgatgtgcta ataaattatg atgcgatgtg ctattgttac
      421 taccattaat acctccgtaa ccatgattcc ccttaggatg atttgaatta tacaaacttt
      481 ctgaaaccat acaccattgt attgttatgt ttggacaaaa gtaaagggac atggtttcgt
      541 caacgctcca actggcagaa acatggtcta aaataatatt actgcaatct cttgcagata
      601 ccgcatccga ttctccacca gattcatttc ctaatctcac ccttaaatag cgtacaatta
      661 catcatgtac tgcgttattg aatactaaag catatttcct caaacaaata ccatctccgg
      721 gagctgtttg tcccgctata gtaagatagg gattagaaat ttttaaatca ctatttaacg
      781 taattgtacc cgaaactcta aatacaactg ttcgaggtcc tgaagcttca actgcagcac
      841 gaagactgcc tgtgcctgaa tcattaaggt tagttacttc ataaactact cctccccgtc
      901 ctccaacggt atattttccg tatccttctg ctcctggaaa ggcaatttgt tgggcataac
      961 taattcccga actcaaaatt aaaagtagca gtactgctat tttgtatata atttttttca
     1021 taattaaaac tgtaatcgat tacattgttg cgtttttcaa atcctaaata ggcgcaacaa
     1081 gataagcatt taaaataaaa caaactcgat tttctgaatg tattggcaaa taaaatctac
     1141 aattgagatt ttattttaga aaccacttgc tcctgactga tcgttcgcat cgcctcttca
     1201 tagccttcca cttttttgtt cccatagact gatgtgggca attttggaaa caaatttcta
     1261 tcggaaacca aagcgttttc caaaggttga ttaaacggcg aaaaacccgc aaaaggatgc
     1321 gtcgctcccc aaagcgtaat gacttttaca cccaacattg ctgcaatatg agcatttcca
     1381 gaatccatag aaagcattac atctaagttg gaaatgagtt gtaattcttc ttgaaactta
     1441 agttttccag caacaacaac tatgttttct ttgccttttg aaagcgaatc taacaattca
     1501 atttcttttt ttccaccacc aaaaagcagg attttatgat tcttttcttc tgccaattca
     1561 ttgataacct cttgcatcaa atccaacgga tagactttgg aatcgtattg tgcaaaaggc
     1621 gcaatcccga ttaatttttg gttattttct ccaatcaaac taaagatttc gctatctaaa
     1681 attgcctttt ctggaaaggt cggatgcgac aattctactt taaaacccaa ttgttcaaaa
     1741 actttgacgt gtctttcgaa catcgtggtc aagggtttga atattttatt ttcggctcgt
     1801 gtcaaggctg ctttttcggc tcttccttta tctacaaatg ctactttttt tccacttaag
     1861 aaaaaaagta ttcgaacgac tttggagcgt aaaacattat gcaaatcagc aaaagcatca
     1921 atctgcaacc ctttcaaatc ttgaaacaat cgcaacaatc caaagaatcc tttgtgacgt
     1981 tgtttttcgt cgaaagcaaa gaaagaaaca ttggaaattc cttcaaaaaa aggtttgaaa
     2041 aatggacgag aaactactgt aattttcacc aacggatatt ggtttacaaa agctcttaca
     2101 acaggaaccg tcatagcgac atctcccatt gctgaaagtc ggaagacggc tatatgtttg
     2161 atttttttgg acaattgatt tatgtttaaa aaccattaag gtattaagaa aattaagaaa
     2221 aacttaatga cccttaatac cttaatggtt taaaatagtg ttcttatttt ttattctgat
     2281 acaaaacagg attcaaatca tcgtcgttgt acattttcat ttgtttgtac actttcataa
     2341 atttatcacc gttttcaatg tcagttagca actcttcaat tgctgttgac aaatcggttc
     2401 tttgctccaa caaaatattc aatttggctt gacatttatc tctgtggtct tgtgaagctt
     2461 ctgcacgagt agcttcttct tgcatatgat aaatttttag agctaaaata gacaatctat
     2521 caaatgccca agctggactt tcggaattga ttttagcatc attttttaca gcaatatggg
     2581 catatttttg aagaaaataa ccgtctatat attctaccat atcggtacgc tcttgattag
     2641 aagcatcaat tcttcttttt aaagtcaaag cagcaacagg atcaatattt gggtcacgaa
     2701 taatatcttc aaaatgccat tgaaccgtat caatccaatt ttttaaatat aataaatgtt
     2761 caattttttc ctttggaaaa ggattgttta taggttgatc tacattatca aattgatgat
     2821 aatcttgaat actttgttcg aaaacagaat aagctaattt tgaaaacata tcttttatat
     2881 tttataaata caaagatact ttttatactt ttatagtcta taaaaaacat aagaagttta
     2941 gcggagtttt tgcgaaattc ctaaatttta atttacaaat cttaaattat cattatgcaa
     3001 acacattatc tatccgaaga caatagcatt ctcaatcatt ttttgggtca aattagaaac
     3061 gtgaatgtgc aacacgacag tatgcgtttt cgaagaaata tagagcgcat tggcgaagtg
     3121 atggcttacg aattaagcaa agatttacag tatagaaata tcgaaattca aacaccgctg
     3181 ggcatcaaaa aaaccactga aatagcagat aaattggttt tatgctccat tcttcgagct
     3241 gggttacctc ttcatttagg ctttctaaat tattttgaca gtgccgaaaa tggttttgtt
     3301 tctgcctaca gacaccaccc aaataacgat gagttttttg atattttagt cgaatatcaa
     3361 gctgttccta atatcgaaaa caagaccttg cttttaatcg atcctatgct tgccacaggt
     3421 caatcgatgg tggctgtttt taacaaatta atggaaaaag gacttccaaa agaaatccac
     3481 attgccgtaa ttattggagc tccagaagga attgcttacc ttgaaaaaca tttgccagat
     3541 tcctgccatc tttgggttgc cacactagac gaaaaattaa atgacaaaaa atacatcatt
     3601 ccgggattgg gagatgcagg agatttggcc tacggaaata aactataatt gagagaaaaa
     3661 cgcaaacaaa ctacacagaa ttaatacgcc caaaaccagt tcttgcttca attgtaattg
     3721 cttaacttcg atgtgactcg ttgccataat agacaatggt gcaatggtat aaattaacat
     3781 atcattgctt ttgtttgatg aaattacaaa aattaataga ccaataaaaa aagccataat
     3841 aatttttttg aacgaagaat tcaataataa tggtttgctt gaaagtgatg caaacataga
     3901 aaccacaaaa aataaagcca ccgttatata aatcgaaaag gcaatattct gataattatt
     3961 tgtaaaataa tcaatattga aattgaaaac tgcattttga ctgatgtaac ctaaaaggtc
     4021 tttgttgcat agtagtgaaa tgcccaccga aataattcca acagcaaaaa aagcaataaa
     4081 cggtaaaacc caattcctat aatcgcgagc aacatggaaa ataacagata taaacaccaa
     4141 aattatataa agaatactcc aaaagtggaa caaagacgcc acaaaaatcc acaatgaagc
     4201 atcgaatatt ttttctttcg tagcttttaa ggattgtagt gaaattaatc ttctaagggc
     4261 taataagata aaaaagttag aagcaattaa gttggtatta ttccaaacgg atggaaaaaa
     4321 taacagaaac aaaaaataaa aaaacaacgt gtagccactg tctttgctca atccgtttct
     4381 cttggcaata aaattggtaa taaaaacaga tgccaacaaa acaagaaaca atccgaatgt
     4441 ttttaaaatg gaaacaaccg atttggtcca agataaatcc tgaaattgat agataaaaaa
     4501 gaaaaccagc attaaaatta caaccaaaat taaatttatt ggcgtagatt ttttaaaaac
     4561 acttgttatc ataaggatat tttatacttt tgcgactgta aatataatac ttgtactaag
     4621 atgaaagcat tttttgaagg aattcaatgg ttatttgaaa caattttttt cgctctacac
     4681 gattttctaa gagaattaga acttacacat tggttcgctg ctaatctttt gaactggatt
     4741 tttatggcca tttgctctgt tgctatcgta tattggtgca aacaattgaa aatctacgat
     4801 gaaaaaggcg aagaaaatca agatacaacc gctcactctt tcttgaaata atattttaag
     4861 aaatttgaaa cgataaatat tgggaactat tttaaatcgt tcccaatatc ttttctaaaa
     4921 tacatcttat caaaatgtag cttgtctata ttttggtaag atttttttat ggcctcttcg
     4981 aagttatctc cataggatgt caccgttaaa actcttccgc cattagaaac tatatttccg
     5041 ttatttagtt tggttccagc gtgaaaaaca atggaatttt ccacgttttc aaagcctgaa
     5101 atcacttttc ctttttcgaa atcttctgga tatccgcctg aaaccaccac aactgttgtg
     5161 gcacttcttt cgtcaatttc caattcgaat tcgtctagtt tttcattggc cacagccaaa
     5221 aacaattcca ccaaatccga tttcattctt gggataacca cttccgtttc cggatcaccc
     5281 attctcacgt tatattcaat aacaataggc tcatttttca cattgattaa tccaataaac
     5341 acaaaacctt tgtattcgat tccgtctttt tggaaacctt caattgttgg tttcacaatg
     5401 cgtgtttcta ttttttccat caaaacagca tccacataag gaactggaga aaccgctccc
     5461 attccacctg tgttcaaacc cgtgtcgcct tcgccaatgc gtttgtaatc tttggcagtt
     5521 ggcaaaattt tatagctttt tccgtcagtc aaaacaaaac agctcaattc aattccatcc
     5581 aaaaactctt cgataactac tttggaacta gccgcaccaa atttttgacc taccaacata
     5641 tttctcaatt cttcttgagc ttctgccaaa tcttgaatga ttaaaacacc ttttcctgcg
     5701 gccaatccat cggctttcaa cacataaggc ggttgcaaag tagcaaggaa ttcgcatcct
     5761 ttctctaccg tttcagccgt aaaactatcg taagccgccg ttggaatctt gtgtttcatc
     5821 aagaattctt tggcaaactc tttacttcct tcgagagttg ctcctaattt tgatggtcca
     5881 ataactggaa tatgttttaa atcatcgtcg tttttgaaaa aatcgtaaat cccttttacc
     5941 aacggatctt ctggtccaac tactaccatt tcaatatttt ctttgataac caaggctttt
     6001 atggcatcaa aatcagtagc gctgatatca atgtttttag caatttgagc agttccagca
     6061 tttcctggag ctacaaaaag agtgtcgcaa agcggacttt gaaccatttt ccaagcaaat
     6121 gcgtgttctc tccctcctga tcctaatagt aaaatattca tagtgtcgtt tttaagtttg
     6181 gcaaaaataa ttgcttttga acttaaaaaa taattaattt ttaaaaagtt actaattcaa
     6241 gagtattagt tgactgtaaa tattattttt ggtgaaatat atccgtaaaa tgtttccact
     6301 tttcgatttt tcgctgctaa taaatggttt tccgatgaag gaagccaaag ccgaattgca
     6361 aaaaatccag tctatcccag aagaagaatt tgaaactttc atcgaaaaca aaaaacaaga
     6421 aattgttgat tatcatttaa aaaataattc ttcctatcaa aatctcgttg gcaaaaccac
     6481 tttcaatact tggaaagatt tgccgataat gaccaagaaa gattttcaaa aacccttgat
     6541 tgaaagactt tcaaccgagt tttctctgaa aaaagtgtac gtcaataaaa cttctggttc
     6601 cagcggtgat ccatttattt ttgctaaaga caaattttcg cacgccttga cttgggcgaa
     6661 tatcattaat cgttttggat ggtatggtat cgattttaat tcttcctatc aagcgcgatt
     6721 ttatggtatt ccgttggatt ttattggtaa caagaaagaa cgtatcaaag atttttttac
     6781 caatcgtttt cgctttccaa tttttgattt atcggataaa gttttagacc gttttttaca
     6841 acaattcaaa ataaaaagat tcgattacat caacggatac acgagttcca tcgttttatt
     6901 tgccaaattt ctacagcaaa aaaatattgt tttaaagaat atttgcccga ctttgaaagt
     6961 tt
//
LOCUS       DAOSNN010000138         6941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_10964_length_6941_cov_5.954981, whole genome shotgun sequence.
ACCESSION   DAOSNN010000138 DAOSNN010000000
VERSION     DAOSNN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10964_length_6941_cov_5.954981"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLQ70_07830"
     CDS             complement(<1..204)
                     /locus_tag="PLQ70_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34560.1"
                     /translation="MKTILSEIKRITKSFSEQAKTFRLLLMVFIFIGINTTIQAQSGI
                     YESYAILKINGGGNAYYNLQASTG"
     gene            416..895
                     /locus_tag="PLQ70_07835"
     CDS             416..895
                     /locus_tag="PLQ70_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HQE34561.1"
                     /translation="MKDIFTIKEAKQKIEHFCAYQERCHEEVISKLWTMKMESDEIDQ
                     IMTHLIAENFLNEERFACSFARGKHRIKHWGKIRITNELKFKNISQTLINIALKELTA
                     EEYLETFHALAERNWASIRETNTLKKRKKFCDFMLRKGFESNLVYDKVKELEGLAHG"
     gene            complement(1082..2236)
                     /locus_tag="PLQ70_07840"
     CDS             complement(1082..2236)
                     /locus_tag="PLQ70_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQE34562.1"
                     /translation="MSQIISITALASISPLGNDPKVVWENYKKENHCFTTQFLDHKQT
                     LVAQLDDNSKVLVEALRQSDIKYKSLDKSVLYAMVASRKAIENSGWTTKDIFGINIGS
                     SRGATDLFEKHFKDYLETGKAQTLASPTTTLGNISSWVAHDLQSLGPEISHSITCSTA
                     LHALLNGVAWLRAGMTDKFLVGGSEAPLTDFTIGQMRALKIYSNSEEAFPNRAFDLEK
                     KQNTLILGEGAAVCCLESGKKGNALAYVEGIGYATEILEHNISISAEATCFQKSMKMA
                     LENADLSEVDAIVMHAPGTRAGDLTEYKAIQKVFGDDLPMLTTNKWKIGHTFGASGML
                     SIELAILMMQHQEFIGVPFAEIQKPRKQIRKVLVNSVGFGGNAVSILLGI"
     gene            complement(2608..4506)
                     /locus_tag="PLQ70_07845"
     CDS             complement(2608..4506)
                     /locus_tag="PLQ70_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQE34563.1"
                     /translation="MSDAIKHECGIALLRLKKPLEFYKEKYGTAFYGIKKMYLLMEKQ
                     HNRGQDGAGFASIKLDVEPGERYISRVRSNAAQPIQDVFAQINDRINEELAANPEYAD
                     NVALQKKEIPYLGELFLGHVRYGTFGKNSIESVHPFLRQNNWMHRNLILAGNFNMTNV
                     TELFQSLIELGQHPKEMADTVTVMEKIGHFLDNAVTDLYQECKNNGLNKREASPVIAE
                     KLDIARILKRASKNFDGGYAMAGLLGHGDAFVFRDPAGIRPAYFYEDDEIVVVASERP
                     VIQTVFNVPFEKVQELQPGNALIIKKNGTVTEEEILVPTVKKACSFERIYFSRGSDAE
                     IYQERKNLGKLVLPAVLEAINDDTDNTVFSYIPNTAETSFYGMIEAAQDFLNQRKNNY
                     ILENRKKLTKEKLEEILAVKIRTEKVAIKDAKLRTFITEDSSRDDLVAHVYDVTYGVI
                     KPTDNLVIIDDSIVRGTTLKMSIIKMMDRLKPKSIVVVSSAPQIRYPDCYGIDMAKLE
                     GLVAFRAALELLKDRNLYHIVDEVYIKCKKQEDLHDNDVVNFVTEIYAPFTPQEISNK
                     ISELLSSPGINAEVKIIFQTVENLHIACPKNLGDWYFTGDYPTPGGNRVVNKAFMNFY
                     EGKNARAY"
     gene            complement(4672..5595)
                     /locus_tag="PLQ70_07850"
     CDS             complement(4672..5595)
                     /locus_tag="PLQ70_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQE34564.1"
                     /translation="MNKLLIVGTVAFDAIETPFGKTDKILGGAGTYIGLSASHFNLES
                     AIVSVVGDDFPQEYLDLLTDRNIDISGLEIVKGGKTFFWSGLYHNDLNSRDTLATELN
                     VLADFQPKVPQKFKTADVVMLGNLHPLVQISVLDQMDVKPKLVVLDTMNFWMDCALPE
                     LMQVIKCVDVITINDEEARQLSGEYSLVKAAAKIHAMGPEYVVIKKGEHGALLFHDHK
                     IFFAPALPLEEVFDPTGAGDTFAGGFAGYITQSENISFENMKNAIIYGSNLASFCVER
                     FGTERMVHLEKDEVIARLQQFKSLTQFDIEI"
     gene            complement(5670..6149)
                     /gene="rnhA"
                     /locus_tag="PLQ70_07855"
     CDS             complement(5670..6149)
                     /gene="rnhA"
                     /locus_tag="PLQ70_07855"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQE34565.1"
                     /translation="MNHDVHIYTDGAAKGNPGRGGYGVVMELVGTPHKKEFYEGFRHT
                     TNNRMELLAVIVGLEKLKNLNMKVLVISDSKYVVDSVMKQWVFGWEKKGFAGKKNPDL
                     WKRFLVVYRKHQVDFKWIKGHNNHPQNERCDELAVFASSQKELSVDVFYEKEDEKLL"
     gene            complement(6159..6728)
                     /gene="purN"
                     /locus_tag="PLQ70_07860"
     CDS             complement(6159..6728)
                     /gene="purN"
                     /locus_tag="PLQ70_07860"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQE34566.1"
                     /translation="MNKILIFASGSGSNAENIIKFFEHKDLAKVVAVFTNNPKAKVIE
                     RAQKFQIPTEIFSKEELNQGKVLQKIIDFAPDLIVLAGFLLKMPKDIIEVYPNTIINI
                     HPALLPKYGGKGMYGMNVHKAIVENKEKETGITIHYVNENYDEGNIIFQKKVTLLVTD
                     TPEVVAEKIHELEQKHFPSVIEDLLTSKL"
BASE COUNT         2197 a         1312 c         1084 g         2348 t
ORIGIN      
        1 tcctgtgctt gcttgtaaat tataataggc atttcctcct ccattgattt tcaatatggc
       61 atagctttcg taaataccag actgagcttg tattgttgta tttatgccta taaaaataaa
      121 aaccatcaac aacaaacgaa atgttttcgc ctgttctgaa aatgattttg ttatcctttt
      181 aatttcagat aaaattgttt tcataacgat actcttttag taaaaacaac acttaacaat
      241 atgaatacta agttattgtg gacaaccaat ttataaacta ttacaacaat tataaaacat
      301 agtcgttgaa atactccttt atctgttata ctactgttta aaaaagctta aacgtcatat
      361 ttgaaagttc aattaaaaac acgaattttg gttgctgatt tgcaaatcaa cttcaatgaa
      421 agacattttc actataaaag aagccaaaca aaagatagag catttttgcg cctatcaaga
      481 gcgttgccat gaggaagtaa tttcaaagtt atggacaatg aaaatggagt cagatgaaat
      541 agaccaaata atgacgcatc ttattgccga aaattttctc aacgaggaac gttttgcctg
      601 tagttttgca agaggaaaac atcgcatcaa acattggggc aaaatcagaa ttaccaacga
      661 attgaagttt aaaaacatct cgcaaacgtt gattaatatt gcgctcaagg aactcactgc
      721 ggaagagtat ttggaaacct ttcacgcatt ggccgaaaga aattgggcat ccatccggga
      781 aacgaatacc ttgaaaaaac gaaagaaatt ctgtgatttt atgctacgca aagggtttga
      841 aagcaatcta gtttacgata aagtgaagga attggaagga ttggctcacg gataacacag
      901 attttctaaa gcaaaacaca gataaaaacg gatttttttc aaaccatata agtcatatta
      961 aggtcattta agttgggaaa acaattaaat tatctgctta tctgcgagaa tccatttaat
     1021 ccgtcactcg agcgatagcg aacaggcaaa gcaaatctgt ggccaaaaat ttgtcttttg
     1081 gttaaattcc caacaaaata ctcaccgcat ttccaccaaa accaactgaa tttacaagga
     1141 cttttcgaat ttgttttctg ggtttttgaa tttcagcaaa tggaacacca ataaattctt
     1201 ggtgttgcat catcaaaatt gccaattcaa tactcaacat tcccgaagca ccaaaagtgt
     1261 gtccaatttt ccatttgttg gtggttagca tcggtaaatc gtctccaaaa actttttgaa
     1321 tggctttgta ttcggttaaa tcacccgctc tagttcccgg agcgtgcatc acgatggcat
     1381 ctacttcaga caaatcagca ttttctaaag ccattttcat cgatttctga aaacaagtcg
     1441 cttcagccga aatggatatg ttgtgttcca atatttcggt ggcataacca atcccttcaa
     1501 cataagccaa tgcgtttcct tttttgcccg attctaaaca gcaaactgct gcaccttcac
     1561 caagaatcaa tgtgttttgc tttttttcca agtcgaaagc ccgattagga aacgcttctt
     1621 cactatttga atatattttc aaagcccgca tttgaccaat cgtaaaatcc gtcaatggcg
     1681 cttcacttcc accaaccaga aatttgtcag tcattccagc acgaagccaa gccacaccat
     1741 ttaacaaggc atgcaaagcc gtagaacaag taatagaatg cgaaatttcg ggacccaaac
     1801 tttgtaaatc gtgggcaacc caagaagaaa tattgcctaa agtcgtcgtt ggcgaagcta
     1861 aagtttgtgc ttttccagtt tctaaatagt ccttaaaatg tttttcgaat aaatcagtag
     1921 ctccacgaga agaaccaatg ttgattccaa aaatatcttt agttgtccag cccgaatttt
     1981 ctattgcttt tctagatgct accatagcat acaaaaccga tttgtctaaa gatttatatt
     2041 taatatctga ttgcctcaaa gcttcgacta atacttttga attgtcatcc aactgagcaa
     2101 caagggtttg tttatggtct aaaaattgag ttgtaaaaca atgattctcc tttttgtaat
     2161 tttcccaaac tacttttggg tcattgccca atggagaaat cgatgcgagt gctgttatgg
     2221 aaataatttg tgacaagtct ttttgttttg tggcaaaggt cgtaaagttt tttattaaat
     2281 ggaacataaa aaacagaaca agaatggtgc aaattcgagt gatttgctca aattttattg
     2341 ttttattttt taaaaagtca aatccaaaat caaaaactta tgttgctgtt tttgtgaatt
     2401 ttgtgttcaa aaaacagcag atacatttta cttgacttta ttccaataaa aaaggtggaa
     2461 ttccttccac cctttttgcc ccaaatctac cataaactta acctactaat attatggtga
     2521 aacaaagata tcttcttaat cgtgtgatga caaatatttt agacgaaata ctaaaatatt
     2581 cgacaaaata cctataatag gattatttta ataagctctt gcattttttc cttcataaaa
     2641 attcataaat gccttattta caacacgatt cccccctgga gttggataat ctcctgtgaa
     2701 ataccaatca cctaaatttt tcggacatgc tatatgtaaa ttttcaaccg tttgaaaaat
     2761 aattttcact tcagcattaa ttcctggcga acttaagagt tctgaaattt tatttgaaat
     2821 ttcttgtggc gtgaatggcg cataaatttc agtaacaaaa tttacaacat cattgtcatg
     2881 taaatcttcc tgttttttgc atttgatata aacttcgtca acaatatgat acaaatttct
     2941 gtctttcaat aattctaatg ccgctctaaa agcaaccaat ccctctaatt ttgccatgtc
     3001 aattccataa caatctgggt aacgaatttg tggagcagaa gaaacaacaa ctatactttt
     3061 gggtttcaaa cgatccatca ttttgataat actcattttg agcgttgttc ctctcacaat
     3121 actatcgtca atgattacca aattgtctgt tggttttatt acaccatagg taacatcata
     3181 aacgtgagcc actaaatcat cgcgactgct gtcctcagtg atgaaagttc ttaattttgc
     3241 atctttgatg gctacttttt cggtacggat ttttaccgcc agtatttcct ctaatttttc
     3301 tttggttaat ttttttctat tttctagaat ataattattt tttctttgat ttaaaaaatc
     3361 ctgagccgct tcaatcattc cgtagaaaga agtttcggca gtatttggaa tataagaaaa
     3421 aacggtgttg tctgtatcat cattaatggc ttcaagaacc gcaggaagaa caagttttcc
     3481 taaatttttt cgttcctgat agatttcggc atcactacct cgagagaaat aaattctttc
     3541 gaatgaacag gcttttttaa cggttggtac aagaatttct tcctcagtta ctgttccatt
     3601 tttcttgata atcaaagcat ttccaggctg aagctcctga actttctcga atggcacatt
     3661 aaaaaccgtt tgaataactg gtcgttctga agcaacaact acaatttcat catcttcata
     3721 aaaataagcc ggacgaattc ctgctggatc tctaaaaaca aaggcgtcac cgtgacctaa
     3781 aagaccagcc attgcataac caccatcgaa gtttttagag gctcgtttca aaattctagc
     3841 tatgtctaat ttttcggcaa ttactggtga ggcttctctt ttattcaagc cgttattttt
     3901 acattcttga tacaaatcgg taacagcatt atctagaaaa tgtccaattt tttccatcac
     3961 tgtaaccgta tcggccatct cttttggatg ttgtcccaat tcaatcaaac tttgaaaaag
     4021 ctcagtcaca ttggtcatat tgaaatttcc ggccaaaatt aaattacggt gcatccagtt
     4081 gttttgacgc aaaaaaggat gaacactttc gatactgttt ttcccaaaag taccataacg
     4141 aacatgtcct aaaaacaatt cgcctaaata aggaatttcc tttttttgca aagctacatt
     4201 atctgcatac tcaggatttg cggccaactc ttcattgatc ctgtcgttta tttgggcaaa
     4261 tacatcttga atgggttgtg cagcattcga cctaactctg cttatgtatc gttctccagg
     4321 ctcaacatct agtttgatgc ttgcaaaacc tgctccatct tgtcctcgat tgtgctgctt
     4381 ctccatcaag agatacattt tttttatccc gtaaaaagca gtaccgtatt tttctttata
     4441 gaattctaac ggttttttta atcttaaaag ggcaatgcca cattcgtgtt taattgcgtc
     4501 gctcattgtg ttgttgtttg tggtttagtt gtattgttag acaattaaat tgtcgtctta
     4561 aattcattaa attaattttc aatctcgttt tttattcgta attgacgtat aaaaattcac
     4621 ttattcctat taatcaaaaa agccccgttt ccgagacttc tttataaaaa gctatatttc
     4681 tatatcaaat tgagttaagg atttgaattg ttgcaatctg gcaatcactt catccttctc
     4741 taggtgtacc attctttcgg ttccaaatct ctcgacacaa aaagaagcga gattagaccc
     4801 ataaatgatg gcgtttttca tattttcgaa tgatatgttt tcactttgtg taatatatcc
     4861 tgcaaaacct cctgcaaagg tatctcctgc tcctgttgga tcaaaaactt cttctagtgg
     4921 caaagctggg gcaaagaaaa ttttgtggtc atgaaacaac aaagcaccgt gttctccttt
     4981 tttaatgact acatattctg gtcccattgc gtggattttt gccgctgctt ttaccaatga
     5041 atattctccc gaaagttgtc ttgcttcttc atcgttaatg gtaattacat caacacattt
     5101 aattacttgc atcaattcgg gcaatgcaca atccatccaa aaattcatgg tgtcaagaac
     5161 tactaattta ggtttaacat ccatttggtc taaaacacta atttgtacta acggatgaag
     5221 atttcctaac attactacat cggcagtttt gaatttttga ggcactttgg gttgaaaatc
     5281 agccaagaca ttcaactcgg ttgccagggt atctctagaa ttcaaatcgt tgtgatacaa
     5341 accactccaa aagaaagttt ttcctccttt aacgatttca agacctgaaa tatcgatgtt
     5401 tctatcggtt aataaatcta aatattcttg tggaaaatca tcaccaacta ccgaaacaat
     5461 ggcagattct agattaaaat gagaagccga aagcccaata taggttcctg ccccacccaa
     5521 aattttgtct gttttaccaa aaggcgtttc aatggcgtcg aaagctactg ttccaacaat
     5581 caataattta ttcatttgat gtgattcgaa ttaagcggca aagatagttt ataagtttta
     5641 gagttgcaaa ggatgaagct ctcaaagttt cataaaagtt tttcatcttc cttttcataa
     5701 aaaacatcca cagaaagttc tttttgactc gatgcaaaaa ctgctaattc atcgcaacgc
     5761 tcattttgag gatgattgtt gtgtcctttg atccatttga aatcgacttg gtgttttctg
     5821 taaaccacca aaaatcgttt ccacaaatcg gggtttttct tgcctgcaaa accttttttc
     5881 tcccagccaa aaacccactg tttcataaca gaatccacga catatttaga atcggaaatg
     5941 accaaaactt tcatattgag atttttcagc ttttcgagac caacaattac agccaacaat
     6001 tccattctat tattagtcgt atgacgaaaa ccttcgtaga attctttttt atgtggtgtt
     6061 cccacgagtt ccatcactac gccatatcca ccacgaccgg gatttccttt tgcagcgcca
     6121 tcagtgtata tatgtacgtc gtgattcaaa atatgtggtt ataatttaga agttagaaga
     6181 tcttctatta ctgatggaaa gtgtttttgt tccaattcgt gtattttttc ggccacaact
     6241 tctggagtat ctgtaactaa tagggttact tttttctgaa aaataatatt tccttcatca
     6301 taattttcat tgacataatg aatggtaatt ccggtttcct tttctttgtt ttcgacaata
     6361 gctttgtgta cattcatacc atacatacct tttcctccat attttggcaa caacgcggga
     6421 tggatattta ttatagtatt aggataaact tcgatgatgt ctttaggcat ttttaataaa
     6481 aaaccagcaa gaacgattaa atctggagca aaatcaataa ttttttgtaa gactttgcct
     6541 tgatttaatt cttctttcga gaaaatttca gtaggaattt gaaatttttg ggctctttct
     6601 atcacctttg cttttggatt gttagtaaaa acagcaacaa ccttcgctag gtctttatgc
     6661 tcaaaaaatt ttatgatatt ttcggcatta gagcccgaac ccgaagcaaa tattaggatt
     6721 ttattcataa ctacattaat ttcatttatg caaaaaaagg aataaaaagt gataataaat
     6781 aacattatga agtctttgtg aaatttattt tataaattag ctgacacatt tattaactaa
     6841 atagttgttt taaaataaag ttttttattt ttgccaacaa attaaaaatt aaaattaaag
     6901 attatgtcag acattgcatc aagagtaaaa gcgattatcg t
//
LOCUS       DAOSNN010000139         6835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11174_length_6835_cov_5.973746, whole genome shotgun sequence.
ACCESSION   DAOSNN010000139 DAOSNN010000000
VERSION     DAOSNN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11174_length_6835_cov_5.973746"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLQ70_07865"
     CDS             <1..491
                     /locus_tag="PLQ70_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQE34567.1"
                     /translation="QGMYDIIGMIWFLRKFHPKFVSKVELGKGIPSVSYNLRHGGSVL
                     IDRKDPKQAIPLIKGMSEYIEKNKRSAVIFPEGTRSKTGKPKEFAQSGLKILCKYAPS
                     AYVVPVSINNSWKFFRFGFFPYGLGNNISFTIHQPLAVKDYPFDEIMEKTENAIVQGI
                     KL"
     gene            558..1544
                     /locus_tag="PLQ70_07870"
     CDS             558..1544
                     /locus_tag="PLQ70_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HQE34568.1"
                     /translation="MSIKNVRLEVMQLLEKNVSDYVDQYLIPVEKIWQPSDFLPNSES
                     DNFLEEVKELREISKDLPYDFWVAMVGDMITEEALPTYENWLMEVEGVDNEARNGWSK
                     WVRQWTGEENRHGDLLNKYLYLSGRVNMKEIEMTTQHLINDGFDIGTGRDPYKNFVYT
                     SFQELATYVSHNRVSQLAKSYGDTKLSKMCKMVAGDEMRHHLAYSEFVNQIFKIDPSE
                     MMLAFQYMMKAKIVMPAHFLRESGQKISSAFEHFSDSAQRIGVYTANDYVEIMQKLIN
                     KWEIDKIGGLTDEAEKARDYLMKLPARMARISERLVIPQESFQFKWVEPARL"
     gene            1729..2382
                     /locus_tag="PLQ70_07875"
     CDS             1729..2382
                     /locus_tag="PLQ70_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014782040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQE34569.1"
                     /translation="MNYSEIVNKTILFVKAKLENAEGGHDWFHIERVYKNALQIADGE
                     VCDTNVVKLGALLHDIADSKFHNGDEAIGPKLAREFLASNNVDKATIQHVIDIIKNVS
                     FKGGNIEKTFSSIELNIVQDADRLDAIGAIGIARAFNYGGFKNRPLYNPNIAPKLHMS
                     KEEYKNSQAPTINHFYEKLLLLKDKMNTETGRKIAQERHHFMEKFLSQFYAEWDGEM"
     gene            complement(2473..2871)
                     /locus_tag="PLQ70_07880"
     CDS             complement(2473..2871)
                     /locus_tag="PLQ70_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34570.1"
                     /translation="MSFQPVFVQFWEKVKESIENKTYAKLTLAKTIGDTELKNIYVRP
                     VLLENDMFSLQLTAKYKTEEIESFHSLEEAFFVLAPYMNNPFLTALLFTTENDITFKL
                     NKKRVGSIIEQAPTFKNASDVILEMKEKRI"
     gene            complement(3040..3822)
                     /locus_tag="PLQ70_07885"
     CDS             complement(3040..3822)
                     /locus_tag="PLQ70_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQE34571.1"
                     /translation="MTLENIILEHEKGIATLFINRPEKLNALNKATIQELHETLKLVN
                     ENPDVRVIIITGSGQKAFVAGADIAEFAHFSAEEGTQLAAKGHQILFDFIENMKTPVI
                     AAINGFALGGGLELAMACHFRIASENAKMGLPETSLGVIPGYGGTQRLAQLVGKGRAM
                     EMILTAGMIDAETAKIYGLVNHVVPQEDLFSAYMTVATRIMKNSPVAISKAIEAVNAN
                     FKTGVNGFDVEIKNFGACFATEDFKEGTTAFLEKRKAEFKGE"
     gene            complement(3835..5250)
                     /locus_tag="PLQ70_07890"
     CDS             complement(3835..5250)
                     /locus_tag="PLQ70_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQE34572.1"
                     /translation="MIVLIIVASILLASISIIQFKNEAKQYHQERLEHKEDAIKENIN
                     YVLSNTTYPLTPSNLALIFKDKIHELSDIHNLEINIYSLDGILLKSSKSRFSIDPVTP
                     PIPKYILKLVQSSIEKRYVDIKTIDGKKNRSSYSLIKDDKFKPLGILNLPYVEDDSYY
                     QAELQSFLIRLAQVYSFMLLVAFGVAYFLSSYITKSLKTISDKLSETSLNQKNEKIVL
                     EANSKEINLLIKAYNGMVDELEESALKLAQSEREEAWREMAKQVAHEIKNPLTPMRLT
                     VQSFQRKFDQNDPNLKQKINDYSETLIQQIDTMSSVASAFSNFASMPVQQNETLNVVD
                     VVELTMDIFNEEYIHFKAEQEEIISKIDRTQLVRIITNLVKNALQAIPENQENKAVCV
                     NIKKASSNVIITIADNGIGIDTHNFNRIFDPKFTTKSSGMGLGLSIIKNIIENYNGTI
                     TFESQKGKGTTFRVSLPLINP"
     gene            complement(5381..5932)
                     /locus_tag="PLQ70_07895"
     CDS             complement(5381..5932)
                     /locus_tag="PLQ70_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HQE34573.1"
                     /translation="MDYYNYLKSLHLIFVITWFAGLFYIVRLFVYQIEANDKPTPEKE
                     ILQKQYKIMTYRLWYIITWPSAVLASIFAFWMLFFTPTGNAWLQMPWMHVKLGFVFVL
                     YLYHYKCHKIYQELQNDQVKYTTNHMRLWNEGATIILFAVVFLVILKNAVNWIYGVVG
                     IMLFSILIMLGFQFYKRIREKSN"
     gene            complement(5933..6448)
                     /locus_tag="PLQ70_07900"
     CDS             complement(5933..6448)
                     /locus_tag="PLQ70_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34574.1"
                     /translation="MIKKVYTIIPFLIFCNFSFGQNQFSEKIISECKIMADAMLKKNY
                     STIIDYTYPKIVEMAGGKAALLKASGQALNNTDSGFELDKITFGEPQKIYVAGKELHC
                     IVPETLIVNTNKGKMQAIYSLLAISQDNGNKWYFIETHNFTPEKIKKIFPNFNYNIVI
                     PENTKPKIIEE"
     gene            complement(6459..>6835)
                     /locus_tag="PLQ70_07905"
     CDS             complement(6459..>6835)
                     /locus_tag="PLQ70_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HQE34575.1"
                     /translation="TPSPAHEFCYRHQCYETTKLVVKALGIPEGKYSQTFQSRLAGDK
                     WLEPYTDVEVNKMPEKGIKKLAVVTPAFVADCLETLEEIAMEANHQFKEHGGENFMAV
                     PCLNDDEEWCKTVAKWLNDWAK"
BASE COUNT         2127 a         1171 c         1262 g         2275 t
ORIGIN      
        1 atcaaggaat gtatgatatt attggaatga tttggttttt gagaaaattt catcccaaat
       61 ttgtcagcaa agtcgaatta ggcaaaggaa ttccaagtgt ttcctataat ttaagacacg
      121 gtggttcagt tcttattgat agaaaagatc ccaaacaagc cattccgctt atcaaaggaa
      181 tgtcagaata catagaaaaa aacaaacgct cggcagtaat ttttcccgaa ggaaccagaa
      241 gcaaaaccgg aaaaccaaag gaattcgctc aaagcggatt gaaaatatta tgtaaatatg
      301 ccccttcagc ctatgttgtt ccagtgagta tcaacaactc atggaaattc ttccgatttg
      361 ggtttttccc ttatggttta gggaataaca tcagttttac catacaccaa ccattagccg
      421 tcaaggatta tccttttgat gaaataatgg aaaaaacaga aaatgctatc gttcaaggta
      481 taaaacttta aaaaagtact aactttgccc tttcttggga gcgatttcaa gttgaaaata
      541 ataaaccaaa aacacagatg tcaataaaaa acgtacgatt agaagtgatg cagcttctag
      601 agaaaaacgt cagcgattat gttgaccagt atttaattcc ggtagaaaaa atttggcaac
      661 catcagattt tttgcctaat tctgaaagtg ataatttctt ggaagaagta aaagaattga
      721 gagaaatatc aaaagatttg ccttatgatt tttgggtggc tatggtaggt gatatgatta
      781 ccgaagaagc tttgcccacc tacgaaaact ggttgatgga agtagaaggg gttgataacg
      841 aagcaagaaa tggttggtct aaatgggtgc gccaatggac tggcgaagaa aaccgtcacg
      901 gcgatttgtt aaataaatat ctttatcttt caggtcgtgt gaatatgaaa gaaattgaaa
      961 tgacaaccca acatctaatt aatgatggtt tcgacattgg taccggaaga gatccttata
     1021 aaaactttgt ttatactagt tttcaggaat tagcaactta tgtttcgcac aacagagttt
     1081 cgcaattggc caaaagttat ggtgatacca aattatccaa aatgtgtaaa atggttgccg
     1141 gagacgaaat gcgtcatcac ttggcttata gcgaatttgt gaatcaaatt ttcaaaattg
     1201 acccgagcga aatgatgctt gcttttcaat atatgatgaa agctaaaatc gtgatgccag
     1261 cacatttctt gagagaatca ggacaaaaaa taagctcggc ttttgaacat ttttcagatt
     1321 ctgcacaacg aattggagtt tataccgcca atgattatgt ggagattatg caaaaactta
     1381 tcaacaaatg ggaaattgat aaaataggtg gtttgactga cgaagccgaa aaagctagag
     1441 attatttgat gaaattacct gctagaatgg caagaatttc agaaagattg gtcattccac
     1501 aagaatcatt tcagttcaaa tgggtagaac cagcgagatt gtagatttta gattgcagat
     1561 tgcagattaa cgaatgcaga tttcagatgg attggaattg attgttgatt gttgattttt
     1621 gccattgaaa ttgtcattgc cattgaaatt gatttttttt aattattgaa tagctgattt
     1681 ttgaatttat attttaaaaa tcagcttttt ttataaattt tttcaaatat gaattattca
     1741 gaaatagtca ataaaaccat actttttgtt aaggcaaagc ttgaaaatgc cgaaggtgga
     1801 cacgattggt ttcacattga gcgagtgtat aaaaacgcct tgcaaattgc cgatggcgaa
     1861 gtttgtgata caaatgtcgt aaaactaggg gctttgcttc acgatatcgc cgatagtaaa
     1921 tttcataacg gagacgaagc cattggtcca aaattagcca gagaatttct agcatccaat
     1981 aatgttgaca aagctactat tcagcacgtt atcgatatca taaaaaacgt ctcttttaag
     2041 ggcggaaata ttgaaaagac attctcgtcc atagaattga atatcgttca agacgccgac
     2101 cgattagacg ccattggcgc cattggaatt gctagagcgt ttaattatgg tggtttcaaa
     2161 aacagaccct tgtacaaccc gaatattgct ccaaaacttc atatgagcaa ggaagaatac
     2221 aaaaatagcc aagctcccac aatcaaccat ttctacgaaa aattattgtt gctaaaagac
     2281 aaaatgaata cggaaacagg gcggaaaatt gcgcaggaaa gacatcattt tatggaaaaa
     2341 ttcctttcgc agttttatgc agaatgggat ggggaaatgt agttggcaat gttcagaagg
     2401 cagtttccag tctaaaaata aattttatct attccaacaa ctgagtactg accactaata
     2461 ctgcaaactt tttcaaatcc tcttttcttt catttccaaa atcacatccg aagcgttctt
     2521 gaaagtgggt gcttgttcga taatacttcc cacacgtttt ttgttgagtt taaaggtgat
     2581 gtcgttttct gtggtgaata acaatgccgt caaaaaagga ttgttcatat agggagccaa
     2641 aacaaaaaaa gcctcttcca gcgaatggaa actttcgatt tcttcggttt tgtattttgc
     2701 cgtaagttga aggctaaaca tatcattttc taacaaaacg ggacgtacat aaatgttttt
     2761 caactcggtg tcgcctatgg ttttggccaa agtcaatttg gcataggttt tattttcgat
     2821 gctttctttt actttttccc agaattggac aaatacaggt tggaaggaca taattgttgt
     2881 ttttgaaaat gttttaaaat ataatgaatt gatatttgaa gaataaacta ctcttcttta
     2941 ctgtctataa agttccgcaa atttaagcga gtttggattg attaatttgc gaatttgcag
     3001 tttaattttt tgaagaccaa ctgaacactg aacacttctc tattcccctt taaactctgc
     3061 ttttcttttc tctaaaaagg cagttgttcc ttctttgaaa tcttcggtgg caaaacaagc
     3121 tccaaaattt ttgatttcaa catcaaatcc gttcacaccc gttttaaaat tagcatttac
     3181 ggcttcaata gctttgctaa tagccacggg agaatttttc ataattcttg tagccacggt
     3241 catataggca gaaaacaaat cttcttgggg tactacgtga ttcactaatc cgtatatttt
     3301 tgcggtttcg gcatcaatca ttcctgcggt tagaatcatt tccattgcac gaccttttcc
     3361 cactaattgt gctaaacgtt gcgttccgcc ataacccgga atcacgccaa gcgaagtttc
     3421 aggcaaaccc attttagcat tctccgaagc gattctaaaa tgacaagcca ttgccaattc
     3481 caatccgcca ccaagggcaa aaccattaat tgcagcaatg acaggtgttt tcatattttc
     3541 gataaaatcg aacaaaattt gatgtccttt tgccgcaagt tgagttcctt cttcggccga
     3601 aaaatgagca aattcagcaa tatcggcacc agctacaaaa gctttttggc cgcttccagt
     3661 tatgataatt acacgaacat caggattttc attgactaat tttaaggttt cgtgtaattc
     3721 ttgaatcgtt gctttgttta acgcattcaa tttttcagga cgattgataa aaagcgtggc
     3781 gatgcctttt tcgtgttcta aaattatatt ttctaatgtc atttgctttt aagtttatgg
     3841 gtttattaat ggcaatgaaa ccctaaaagt agttcctttt cctttttgag attcgaatgt
     3901 aatagttccg ttataatttt cgataatgtt tttgatgata ctcaaaccca atcccattcc
     3961 gctggatttt gtggtgaatt ttggatcaaa aattcgattg aaattatgag tatcaattcc
     4021 aattccgttg tcggcaattg ttataatgac attgctggat gcttttttga tgttgacaca
     4081 aactgcctta ttttcttgat tttctggaat ggcttgaaga gcatttttga ccaaattggt
     4141 aataattcga acgagttgtg tacggtcaat tttcgaaata atttcttctt gttcggcttt
     4201 gaaatggatg tattcttcgt taaaaatatc cattgttaat tccaccacgt ccaccacatt
     4261 caaagtttcg ttttgttgaa ctggcatcga agcgaagttt gaaaatgccg aagccacaga
     4321 actcatcgtg tcaatttgct gaattaaggt ttcggaataa tcgttgattt tttgttttag
     4381 attcgggtcg ttttgatcaa actttcgttg aaaactttgt accgtcaatc gcattggcgt
     4441 tagcggattt ttgatttcgt gcgccacttg tttggccatt tctcgccaag cttcttctcg
     4501 ttcgctttgg gctaatttga gggcactttc ttcgagttca tcgaccattc cgttataagc
     4561 tttaatcaaa agattgattt ctttgctgtt ggcttcgagt acaatttttt cgtttttctg
     4621 attcaaactc gtttcgctca atttatccga aatggttttt aacgattttg tgatatagga
     4681 agaaagaaaa taagccaccc caaaagctac caaaagcatg aaactataca cttgcgccaa
     4741 tctaatcaag aaactttgta gttcagcttg gtaatagctg tcgtcctcta cataaggtaa
     4801 attcaggatt cctaaaggtt tgaatttgtc atctttgatt aagctatagg acgaacggtt
     4861 cttttttcca tcaatggttt tgatgtcaac gtatcttttt tcgatagaag attgaaccag
     4921 ttttagaata tattttggaa ttggaggggt tactgggtca atagaaaatc ttgattttga
     4981 agatttcaaa agtattccgt caaggctata aatattaatt tcaagattgt gaatatcgga
     5041 caattcgtga attttatcct taaaaatcaa ggctaaatta ctaggtgtta gcggataagt
     5101 ggtgtttgaa agcacataat tgatgttctc ttttatggcg tcttctttat gctctaagcg
     5161 ttcttgatgg tattgcttgg cctcattttt gaactgaatt atagaaatgg atgctaataa
     5221 tatggaagcc acaataatca atactatcat cgaaaggaaa atcctgattc gcaacgaaag
     5281 cattgatatt ttgaaggttt ttagcatttt tttagtgatt agcgaatagt aattagtgaa
     5341 tagtcaaggg cttttaaagc taattactat tcgctaatta ctaattactt ttttctcgaa
     5401 ttcttttata aaattgaaag cccaacataa tcaagataga aaacaacata attccgacca
     5461 ctccataaat ccagtttacg gcatttttca gaatgactaa aaacactacg gcaaatagga
     5521 taattgttgc tccttcgttc cacaaacgca tatgatttgt ggtgtatttt acttggtcgt
     5581 tttgcaattc ttggtatatt ttgtggcatt tgtaatggta taaatacagc acaaaaacaa
     5641 agccaagttt tacgtgcatc caaggcattt gcaaccaagc atttcccgta ggggtaaaaa
     5701 acagcatcca aaacgcaaaa atactagcca aaaccgctga tggccaagtg ataatgtacc
     5761 acaaacgata ggtcattatt ttgtattgtt tttgcaagat ttctttttcg ggcgttggtt
     5821 tgtcgttggc ttcaatttga tacacaaata aacgaacaat ataaaaaagt ccggcaaacc
     5881 aagtgatgac aaagatgagg tgcagggatt tgaggtagtt gtagtagtcc atttattctt
     5941 caatgatttt aggtttggta ttttcaggaa taacaatgtt gtaattaaag tttggaaata
     6001 tcttttttat tttttcaggt gtgaaattat gagtttctat aaaataccat ttgttcccat
     6061 tatcttgact aattgctaac agagaataga tcgcctgcat ttttccctta tttgtgttta
     6121 ctatcaatgt ttcaggaaca atgcaatgta attcttttcc agcaacatat atcttttgag
     6181 gttcgccaaa agtaatttta tctagttcaa atccgctatc agtattattt aatgcttgtc
     6241 cagatgcttt tagcaaagct gcttttccac ctgccatttc aactattttg ggataggtgt
     6301 aatcaataat agttgaatag ttttttttaa gcattgcgtc agccattatt ttacattcag
     6361 atataatttt ttctgaaaat tgattttgcc caaaagaaaa attacaaaaa attaagaaag
     6421 ggataattgt gtaaactttt ttaatcataa taagtgtttt attttgccca atcatttaac
     6481 cattttgcca ccgttttgca ccattcttca tcgtcattca aacacggaac tgccataaaa
     6541 ttttcacctc catgttcctt gaattggtgg ttggcttcca ttgcaatttc ttccaaagtt
     6601 tccaaacaat cggccacaaa agcgggtgtc acgacagcca attttttaat tcctttctcg
     6661 ggcattttgt tcacttcgac atcggtgtag ggttcgagcc atttgtcgcc tgccaagcgc
     6721 gattgaaaag tctggctgta tttgccttcg ggaataccta atgcttttac gaccaatttc
     6781 gtggtttcat aacattgatg acgatagcaa aactcgtgag ctggcgatgg cgtgt
//
LOCUS       DAOSNN010000140         6832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11178_length_6832_cov_4.054744, whole genome shotgun sequence.
ACCESSION   DAOSNN010000140 DAOSNN010000000
VERSION     DAOSNN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11178_length_6832_cov_4.054744"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLQ70_07910"
     CDS             complement(<1..550)
                     /locus_tag="PLQ70_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34576.1"
                     /translation="MKNQFIITVAFIAVTSFTNSLLAQQPAAKTAGYDLKTAKGLSCS
                     VVSTDQGCSLNFTKIEMSYRDGASGMATGKRQHKPFVVIYDVSSSDNSVSQVKSPRDA
                     ASGQATGKRTSSVTSGMSEVSSSSTVQGSGGGAGKVSMQDFHFSMKGNGKTISLSCPD
                     GSCDISTDLPDGQYTLSCDWSWG"
     gene            complement(716..1171)
                     /locus_tag="PLQ70_07915"
     CDS             complement(716..1171)
                     /locus_tag="PLQ70_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34577.1"
                     /translation="MIALIGAFFMGALAFAQVSKNDTINKTASRKVMSNDLKNMPSDA
                     DNEVKSDAIKRGYNQKTSSQQNGYMKISDQTSQSSKATVIQKGKSSATSMSDETITQK
                     GREGKMTTTEKQHYTIKMSNADKKVVKDSISKPTKSSDTFIKISDVKGE"
     gene            1162..1620
                     /locus_tag="PLQ70_07920"
     CDS             1162..1620
                     /locus_tag="PLQ70_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HQE34578.1"
                     /translation="MRSFFSYFKKFKSDKNIFFVTKPNNSIYVAISTIHGRGVFAQHP
                     IKVGSIIEECPIIKMKLQEMTVRKHMLLNYYAFMIDEQSEYTGIALGYGSLYNHSDNC
                     NAIYYFNKDKELMIFEAVQPIAKDEEITINYLGPESAGQSIESWFNKPEL"
     gene            complement(1867..3717)
                     /locus_tag="PLQ70_07925"
     CDS             complement(1867..3717)
                     /locus_tag="PLQ70_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HQE34579.1"
                     /translation="MKKNALLLLIVFLITNIVSSQESCYNLDFETGTLVGWEGGTGQC
                     CPVEIKYSGIIRGRHTIMKGKGVDPNTCDNLSVVAPGGSYSARLGNDSTGKEAESLSY
                     TVDITEANSLFIYKYAVVLQDPGHKPEEQAYFRVNIYNDNRELIDPTCGSYNVIATSN
                     LPGFKTCDKYGVVYKDWTTVGLDLSPYIGKNITVEFETSDCSLGGHYGYAYVDAYCSS
                     LRIGSSYCVDSNGVTLSAPTGFEYLWETGETTQTIKIDNPIDGKKYICELTSVTGCKV
                     NISTVLNLQDPIINFEVANACDKKEVVFTNTTLNTDNTLNTFLWDFGDGTTSTVENPK
                     HTYAAPGKYNVTFEFKNILGCEFEMTKAVTINLTPEPHLSDGVVCLDSTGNLVNGYVL
                     DTGLSDVENEYQWFLNGTLLFGDIKSTYTALEKGKYSVLVTNSKSGCSNQAFASVTSS
                     IMASDFIANVSEAFATIGSVNVQVVGGTGPFLFKLDDNEFQESNVFNGLPYGEHNITV
                     KDSVNCTLITKPITIMGYPKFFTPNNDGINDYWNIPNFKNFYQAQIYIFDRYGKLIKE
                     ISPKELGWDGTRLGNFMPASDYWFTLNYTEMDQNGDLKSKTFKSHFSLKR"
     gene            complement(3950..5350)
                     /gene="glmM"
                     /locus_tag="PLQ70_07930"
     CDS             complement(3950..5350)
                     /gene="glmM"
                     /locus_tag="PLQ70_07930"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQE34580.1"
                     /translation="MTLIKSISGIRGTIGGKVGENLTPVDAVKFASAYGTWLKQQTIN
                     DKFKTNFKVVIGRDARISGPMIHNLVVNTLIGLGIDVIDLGLSTTPTVEVAVPLESAD
                     GGIILTASHNPKQWNALKLLNEKGEFLNGIEGEKILQIAEAEAFDFSDVDNLGEIIEN
                     NAYMDIHIDEVLNLPLVDIEAVKAAKFKVIVDGVNSSGGIIIPKLLELMGVEVVKLYC
                     EPNGHFPHNPEPLKEHLTDISELVVEKKADLGIVVDPDVDRLAFISEDGEMFGEEYTL
                     VACADYVLSKTPGNTVSNMSSSRALRDVTNLHNGSYEASAVGEVNVVELMKKNNAIIG
                     GEGNGGIIYPELHYGRDSLVGVALFLTHLANKKMSVSALRASYPEYYMSKNKIELTPQ
                     IDVDAILVAMTEKYKNEDIATIDGVKIDFAENWVHLRKSNTEPIIRIYTEAASQEKAD
                     ALALKIIDEIKVIAGI"
     gene            5440..6024
                     /locus_tag="PLQ70_07935"
     CDS             5440..6024
                     /locus_tag="PLQ70_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HQE34581.1"
                     /translation="MNFLAHIYLSGENDLIKIGNFMADGIHGKHFDSFPIEIRKGIIL
                     HREIDTFTDSHPLFRQSTKRLHANYHHYSGIIVDIFYDHFLAKNWKNYSDEKLEDYTE
                     RFYQSLRDNFEVLTPKTQKMVPYLVTQNWLMSYQTVEGIEIILEKMDNRMKRNSNMRF
                     AVAELRTFYSEFEEEFTLFFKEIQSYSNQKIQTL"
     gene            6028..6696
                     /locus_tag="PLQ70_07940"
     CDS             6028..6696
                     /locus_tag="PLQ70_07940"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HQE34582.1"
                     /translation="MSFIQNLENAFQENSNPENALAMAKYMRNYFSFYGIKTDQRRLV
                     FKSIWSENQIEVSAHSREIALQLFLKPERELHYCGLEILIKQLKRNYRIEDIQLIEKL
                     LITNSWWDSVDTISKNILGQYLLEHPQEIEKVIERFSNSDNMWLNRSAILFQLGFKQK
                     TNFDLLKSECEKHKNSNEFFIQKAIGWALREYAKTNPQAVKNYVANTNLKNLSQKEAL
                     KNIL"
BASE COUNT         2307 a         1251 c          967 g         2307 t
ORIGIN      
        1 aaccccagct ccaatcacaa gataatgtgt attgaccatc aggcaaatca gttgaaatat
       61 cacaactgcc atcaggacag gataaactta ttgtttttcc gttacctttc atcgagaaat
      121 gaaaatcttg catcgaaact tttcctgctc caccacctga accttgaact gttgaactag
      181 aacttacttc actcattcca gatgttactg aagatgttct ttttcccgtt gcttgtccgc
      241 tagctgcatc tcttgggctt ttcacttggc taactgaatt atcacttgaa ctcacatcat
      301 atattacaac aaaaggtttg tgctgtcttt ttccagtagc cattccgctg gcaccatctc
      361 tgtaagacat ttcaattttt gtgaaattaa gcgagcaacc ttggtctgta ctaacgacac
      421 tgcaactaag tccttttgca gttttgagat cataaccagc agttttggca gcgggttgtt
      481 gtgctaataa tgaatttgta aaacttgtta cggcaataaa tgctactgta ataataaatt
      541 gatttttcat gatttctaaa gttaaaatgt tagtttattt gttgttatag tagtagatac
      601 tttaagtcca aaaattgtta ccccttaaaa ttaaaaatat taaaaaaaat agtactgaat
      661 tgttttccaa aaatgtaaac agctaaaata catatggtta caaattaaaa aaatattatt
      721 ctccttttac atctgatatt tttataaaag tatccgaaga ttttgtaggc ttagaaatac
      781 tgtctttaac aactttctta tctgcattac tcatttttat ggtataatgt tgtttttctg
      841 tagttgtcat ttttccctct cttcccttct gagtaattgt ttcatctgac attgatgtag
      901 cagaagattt gcctttttgg ataactgttg ctttactact ttgagaagtt tgatcagaaa
      961 ttttcatata tccgttttgt tgactactcg ttttttggtt atatcccctc ttaattgcat
     1021 cgcttttaac ctcattatca gcatctgaag gcatattttt caaatcatta ctcattactt
     1081 tcctactagc tgttttattt atagtatcat ttttgctaac ttgcgcaaac gctaaagcac
     1141 ccataaaaaa agctccaatt aatgcgatca tttttttcat atttcaagaa atttaaaagt
     1201 gataaaaata ttttttttgt tactaaaccc aataattcta tttatgttgc catttcaact
     1261 attcacggtc gtggcgtttt tgctcaacat cctattaaag ttggctccat tattgaagaa
     1321 tgtcccatta taaaaatgaa acttcaggaa atgacagtta gaaagcatat gttgttaaac
     1381 tattatgctt ttatgattga tgaacaaagc gaatataccg gaattgcact aggttatggc
     1441 tctttataca accattcaga caattgcaat gccatttatt atttcaacaa agacaaagaa
     1501 cttatgattt ttgaagcagt tcaacccatc gcaaaagatg aggaaattac tataaattat
     1561 cttgggcctg aatctgctgg acaatctatt gaaagctggt ttaacaaacc tgaattgtaa
     1621 tctattttct taaaaaactt tataaatata caagttttta tttaacacat aaaaaaacac
     1681 acaccttttt gttaaaaatg taaaaaaaaa tgcatccaat atataaaaac attgaatgca
     1741 tttttttaac taattctgct ttttggatta actattactc aaattccagc aataattttg
     1801 aattcatcaa taatttgaga aataaaaatt actacttact gtcatcaaat gggctttgat
     1861 gtaaaattat cgctttaaag aaaaatgaga cttaaaagtt tttgatttta aatccccatt
     1921 ttgatccatt tctgtataat tcaaagtaaa ccaataatct gaagctggca taaaattacc
     1981 aagacgagta ccgtcccatc ctaattcctt tggagatatt tcttttatca atttaccata
     2041 tcgatcaaaa atataaatct gtgcttgata gaaatttttg aaatttggaa tattccaata
     2101 atcattaata ccatcattat taggtgtgaa aaatttggga tatcccataa tagtaattgg
     2161 tttagtgatt aaagtacaat taacactatc ctttactgta atgttatgtt ctccgtatgg
     2221 caatccatta aaaacattgg attcttgaaa ttcattatcg tctaatttaa aaagaaaagg
     2281 ccctgttcct ccaacaacct gaacatttac agaaccaatt gttgcaaaag cttcactaac
     2341 atttgctata aaatcactag ccattatcga tgaagttact gatgcgaaag cttgattaga
     2401 acagcccgat tttgaatttg taaccaatac ggaatatttt cccttttcaa gagcggtata
     2461 agtgcttttt atatcgccaa aaagtaacgt accattcaaa aaccattgat attcattttc
     2521 aacatcagat aaaccagtgt ccaaaacata accatttact aaatttccgg ttgagtctaa
     2581 acaaactaca ccatcgctca aatgaggttc tggagttaga tttatagtca ctgctttggt
     2641 catttcaaat tcacaaccta aaatgttttt aaattcgaat gttacattgt actttccagg
     2701 agcagcataa gtatgtttag gattttcaac tgtagaagtt gttccatcac caaaatccca
     2761 aagaaaagta tttaatgtat tatcagtatt taaggtggta tttgtaaaaa caacctcttt
     2821 tttatcgcaa gcattagcaa cttcaaaatt aattatagga tcctgaaggt ttaaaactgt
     2881 tgaaatatta accttacatc ctgttacaga tgtcaattcg caaatgtatt tttttccgtc
     2941 aatagggttg tcaattttta ttgtttgggt agtttcaccc gtttcccaca aatactcgaa
     3001 acctgtagga gctgaaagag taaccccatt agaatcaaca caataactag aacctattct
     3061 aagcgaggaa caataagcat ctacataagc atatccataa tggcctccta gcgagcaatc
     3121 actagtttcg aattctacag taatattttt accgatataa ggacttaaat ccaatcctac
     3181 agtagtccaa tctttataaa caactccata tttatcacaa gttttaaaac caggaagatt
     3241 gcttgtagca attacattat aagaaccaca agtaggatct atgagttctc tgttatcatt
     3301 ataaatgttt accctaaagt aggcttgctc ttcgggttta tgacctggat cttgtaaaac
     3361 aacggcgtat ttataaataa ataaggaatt ggcttctgta atatcgacag tataagatag
     3421 tgattcggct tctttgcccg tgctgtcgtt acctaatctt gcagaatagg aacctccagg
     3481 agctacaact gacaaattat cgcaggtatt gggatcaact ccttttccct tcataatagt
     3541 atgcctacct ctaataattc ctgaatattt gatttctact ggacaacatt gtcccgttcc
     3601 cccttcccaa ccaacaaggg tacccgtttc aaaatctaaa ttataacagg attcttgtga
     3661 agaaactatg tttgtaatta gaaaaactat taataaaagt agtgcgtttt ttttcataac
     3721 agttttctat tcgtttcaaa atcagatgga agttatttta ctgatttcat ttcctaatta
     3781 caccccttgg agattacaaa attaatacta atttttatct tattgctaat ttgtatattc
     3841 ttacgcaaaa atcttaccaa aaattacttt ttcaaaaaaa acatctaata ctaaatataa
     3901 aagccgtttt aaattttact caaaaacggc ttttaataaa ctatgaaagt taaatccctg
     3961 ctataacctt tatttcatca atgattttca aggccaaagc atcggctttt tcttgcgaag
     4021 ctgcttctgt ataaattcga ataataggtt ccgtattgga ttttcttaga tgcacccagt
     4081 tttcggcaaa atcaattttc acaccgtcaa tcgtagcaat atcttcgttt ttatattttt
     4141 cggtcattgc caccaaaata gcatcaacat caatttgtgg tgtcaattca attttgtttt
     4201 tactcatata gtattctgga tatgaagctc tcaaagcaga aacactcatt tttttgttgg
     4261 ccaaatgcgt caaaaacaag gcaacaccta ccaaactatc acgaccgtaa tgcaattcag
     4321 ggtaaataat tcctccattg ccttctccac ctatgatggc attgtttttc ttcatcaatt
     4381 cgacaacatt cacctcgcct actgcgctag cttcgtagct accattatgt aaattagtca
     4441 catcacgcaa agcacgcgaa gaagacatat ttgaaactgt attgcctgga gttttactca
     4501 aaacataatc tgcacaagct accaacgtat attcttcgcc aaacatctct ccgtcttcag
     4561 atataaaagc caaacgatct acatctggat caacaacaat acctaaatcg gcttttttct
     4621 cgacaaccaa ttctgaaata tcggttaaat gttctttcaa aggttctgga ttgtgaggga
     4681 aatgtccgtt aggttcacaa tacaatttca cgacttcaac gcccattaat tctaataatt
     4741 ttggaataat aattccgccc gatgaattca caccatcaac aatcaccttg aatttagccg
     4801 cttttacagc ttcgatatct accaatggca agttcaaaac ctcatcgata tgaatatcca
     4861 tataagcatt attttcaata atttcgccca aattgtccac atccgaaaaa tcaaaagctt
     4921 cggcttcggc aatttgaagg attttctctc cttcaattcc atttaggaac tccccttttt
     4981 cattcaacaa tttcaaggca ttccattgtt ttggattgtg cgaagcagta agaataattc
     5041 ctccatcggc actttctaaa ggcactgcta cttcaacagt tggcgttgtc gaaagcccta
     5101 aatcgattac atcaattcct aaaccaatta atgtatttac caccaaatta tgaatcattg
     5161 gccccgaaat acgagcatca cgaccaatga caactttaaa atttgttttg aatttatcgt
     5221 tgattgtttg ttgttttaac caggttccat aggccgaagc aaattttact gcatcaacag
     5281 gagtcaggtt ttcgcctact tttccgccaa tagtccctct aattcctgat atcgatttta
     5341 ttaaagtcat tttgaattaa atttagattt taaaattttt gactacaaat ataataaaca
     5401 cagtttagaa aagtcattta aaattcatag attagtcgaa tgaattttct agcacatatc
     5461 tatctttctg gcgaaaacga cttgataaaa attggcaatt ttatggccga tggcattcac
     5521 ggaaaacact tcgattcttt tcctatagaa attcgaaaag gaattatttt gcatcgagaa
     5581 attgatactt ttaccgattc acatccttta tttagacaaa gcaccaaacg tttgcacgct
     5641 aattatcatc attattctgg aattattgtc gatatttttt acgaccattt cttggcaaaa
     5701 aactggaaaa attattcgga tgaaaaattg gaagattata cagaacgctt ttaccaatct
     5761 ttgagagata attttgaagt tcttactcct aaaacccaaa agatggtgcc ttatttagtt
     5821 actcaaaact ggttaatgag ttaccaaacc gttgaaggca ttgaaataat tttagaaaaa
     5881 atggataatc gaatgaaacg caattcgaat atgcgatttg ctgtagctga attgagaact
     5941 ttctactctg aatttgaaga agaattcacc ttgtttttta aagaaataca atcctactcc
     6001 aaccaaaaaa tacaaaccct ataaccaatg agtttcattc aaaatttaga aaacgctttt
     6061 caagaaaata gtaatcctga aaatgctttg gctatggcca aatatatgcg aaattatttt
     6121 tcgttttatg gaataaaaac cgaccaaaga agacttgttt ttaaatcgat ttggagcgaa
     6181 aatcagatag aagtttcagc tcattctagg gaaattgctt tgcaattatt tttaaaacca
     6241 gaacgtgaat tgcactattg cggacttgaa atcctgataa aacaattgaa aagaaattat
     6301 agaattgaag atattcaact aattgaaaaa ctattaatta ccaactcctg gtgggacagt
     6361 gtcgatacga tttcaaaaaa tattttaggt caatatttac ttgaacatcc gcaagaaatc
     6421 gaaaaagtga tagaacgttt ttcgaattca gacaatatgt ggctcaaccg cagtgcgatt
     6481 ctttttcaat tgggattcaa gcaaaaaacc aatttcgatt tattgaaatc agaatgtgaa
     6541 aaacataaaa attccaacga gttttttatt caaaaagcca taggctgggc attacgagag
     6601 tatgcaaaaa cgaatcctca agcagtaaaa aattatgtag ccaacactaa tttaaaaaat
     6661 ttaagtcaaa aagaagcctt aaaaaacatt ctttgatttc taaataagta tttttgtaca
     6721 cttctaaaaa agcaaaatgt taaagaaaac aatttataga attgaaaacc taattgcctg
     6781 gtcgcgggat aaaatcacaa acaaacaatt tatattttta tctagtgttt tg
//
LOCUS       DAOSNN010000141         6781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11298_length_6781_cov_4.291258, whole genome shotgun sequence.
ACCESSION   DAOSNN010000141 DAOSNN010000000
VERSION     DAOSNN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11298_length_6781_cov_4.291258"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLQ70_07945"
     CDS             complement(<1..345)
                     /locus_tag="PLQ70_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HQE34583.1"
                     /translation="MGTFVISKRFDDRYKFVFTSRKGKTIFTSPSLELKMDCEDSIEA
                     IKANIEKYTYVKSRAANGKYFFKMMLEETIIATSRKYTTPLLLEKGIDEIIRTAVKSD
                     VLDFTMNEFVFPD"
     gene            complement(349..951)
                     /locus_tag="PLQ70_07950"
     CDS             complement(349..951)
                     /locus_tag="PLQ70_07950"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQE34584.1"
                     /translation="MSINSSFPQNTILEDDFVLLRPLQEFDVDNLLEIAINEPETWQY
                     SLVPAHGKENLTKYIQHAVKSRENKTEFPFIVFDKKSGKYAGSTRFYDIQLPFKTLQL
                     GYTWYGKDFRGTGLNKHCKFLLLQFAFETLGMERVEFRADNNNVRSIAAMKSIGCQVE
                     GVLRNHMPTFESEVRRDSIILSILRNEWFDSVKENLKSKL"
     gene            complement(1022..1378)
                     /locus_tag="PLQ70_07955"
     CDS             complement(1022..1378)
                     /locus_tag="PLQ70_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34585.1"
                     /translation="MYELLFWQYQDGIYLNHHLVYEALVENQEVEGLEILPVQVILNR
                     INSVFSTWEKVDENSWKNTKGKGAFQIKTTPQSIQIDCYGTEGKTMNLLADTLEEFKC
                     PLYDPQVPVRYDEFNE"
     gene            complement(1512..2690)
                     /locus_tag="PLQ70_07960"
     CDS             complement(1512..2690)
                     /locus_tag="PLQ70_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HQE34586.1"
                     /translation="MSSDSSKRYAQRGVSASKEDVHNAIKNIDKGLFPQAFCKIVPDY
                     LTNDEDYCLIMHADGAGTKSSLAYMYWKETGDISVWKGIAQDALIMNIDDLLCVGAID
                     NILLSSTIGRNKNLIPAEVISAIINGTEELIKELDSFGVTIHSTGGETADVGDIVRTI
                     IVDSTVTARMKRSKVIDNANIKAGDVIVGLESFGQATYEKSYNGGMGSNGLTSARHDV
                     FGKYLAAKYPESYDAAVPEELIYSGQVKLTDAVENSPIDAGQLVLSPTRTYAPIIKKI
                     LDKYDSKDIHGMVHCSGGAQTKILHFVQNLHIIKDNLFPVPPLFKLIQEQSKTDWKEM
                     YQVFNCGHRMEIYVPEAVAQDIIAISKSFNVNAQIVGRVEAADAKKLTISSEYGIFEY
                     "
     gene            2959..3231
                     /locus_tag="PLQ70_07965"
     CDS             2959..3231
                     /locus_tag="PLQ70_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HQE34587.1"
                     /translation="MPKYVIEREIPGAGKLSPEQLKGISQTSCGVLNKMGSQIQWVHS
                     YVTGDKIYCIYNAPNEEMVCEHAKLGGFPANSVNEVATIIDPITAE"
     gene            complement(3301..5490)
                     /locus_tag="PLQ70_07970"
     CDS             complement(3301..5490)
                     /locus_tag="PLQ70_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HQE34588.1"
                     /translation="MATLRFQALNEASSRKPVHFEETDRKSIIFGSNVFNEKAMKQYL
                     TSDALKAVQSAVLNGTKIDRKLADYIAMGMKEWALSKGVTHYTHWFQPLTGTTAEKHD
                     AFFETSYDGSESVEKFGGGQLVQQEPDASSFPNGGIRNTFEARGYTAWDPTSPAFIYG
                     TTLCIPTVFISYTGEALDNKIPLLRALSAIDEAATEVCKYFDKNVKKVTATLGWEQEY
                     FLVDSALANSRPDLIMTGRTLLGHTSAKGQQLDDHYFGSIPTRALTYMRDLEQECMLL
                     GIPVKTRHNEVAPNQFELAPIFEETNLAVDHNCLLMDVMQKVAERHDFKVLFHEKPFK
                     GVNGSGKHNNWSLATDTGVNLLSPSKTPMSNLQFLAFFINTIKAVNDYESLLRGSIAT
                     ASNDHRLGANEAPPAIISVFIGEQLTKVLAELEGVTDGKLSPEEKTDLKLNVVGKIPE
                     VILDNTDRNRTSPFAFTGNKFEFRAVGSSANCSNAMTTLNTIVAKQLKDFKVEVDALI
                     DNKGLKKDEAIFNVLREYIKLSKKILFEGDGYSDAWEKEAAKRGLSNHKTTPEALKAR
                     ASKQALDLFSEMGIMNHVEVEARYEIELEEYTKKIQIEGRVLGDLSQNHVIPTAIRYQ
                     NTLIENVKGLKEIFGDEFKTLAKEQIILIREVSGHIEGINSKVEAMTNERKKANHLDN
                     AQAMAEAYCNNVKPYFEEIRNHCDKLELLVDDENWTLTKYRELLLTK"
     gene            5693..6706
                     /locus_tag="PLQ70_07975"
     CDS             5693..6706
                     /locus_tag="PLQ70_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HQE34589.1"
                     /translation="MAKIKLEYLWLDGYEPTQNLRSKTKVEEHENFQGTLEEIGNWSF
                     DGSSTRQAEGGSSDCMLVPVAIYPDPTRINGYLVMTEVMNADGTPHASNGRATIDDDG
                     DFWFGFEQEYFIMDTKTLLPLGFPVGGYPAPQGMYYCSVGGKNTHGRKLVEEHADLCI
                     AAGINFEGINQEVACGQWEFQLFAKGAKKAGDEIWVARYLLDRLTEKYGYYIEYHPKP
                     LGDTDWNGSGMHANFSNEVLRTCGSKETYDKICEAFRPVTEEHIAVYGAYNEQRLTGK
                     HETASIHDFSYGVSDRGCSIRIPLITVQKGYKGWLEDRRPASNGDPYKIAARIIKTVK
                     SAL"
BASE COUNT         2014 a         1306 c         1236 g         2225 t
ORIGIN      
        1 atccggaaac acaaattcat tcatcgtaaa atctaaaaca tccgatttta cggcggtgcg
       61 aatgatttcg tcaatgcctt tttcgagtaa taaaggcgtg gtgtattttc ggctggtggc
      121 tattatggtt tcttccagca tcattttaaa aaagtatttt ccgttggcgg ctctcgattt
      181 tacataagta tatttttcga tgtttgcttt tatggcttcg atgctatctt cgcaatccat
      241 cttgagttct aatgacggac tggtaaaaat agtttttcct tttcgggaag taaatacaaa
      301 tttgtaacga tcatcaaacc gtttgctgat aacgaatgtg cccataaatt agagtttact
      361 tttcagattt tcttttacag agtcaaacca ttcgttgcga agaatactca aaataatact
      421 atcgcgtcta acttcactct caaaagtggg catgtgattc cgcagcacgc cttcgacttg
      481 gcaaccaata cttttcattg cagcaatact tcgcacattg ttgttgtcgg cacgaaattc
      541 tactcgttcc attccaaggg tttcgaaagc aaattgcaaa aggagaaatt tgcaatgttt
      601 gtttaatcct gttcctctga aatcttttcc ataccaagtg taacccaatt gcagggtttt
      661 gaacggtaat tgaatatcat aaaaacgagt acttccagca tattttccag atttcttgtc
      721 gaaaacaata aagggaaatt cggttttgtt ttctctggat tttacagcgt gctgaatgta
      781 tttcgtcaaa ttttcttttc cgtgtgcggg aactaatgaa tattgccaag tttcgggttc
      841 gttgatggcg atttccaata aattatcaac gtcaaattct tgcaacggac gaagcaaaac
      901 aaagtcgtct tcgaggattg tattttgtgg gaagctagaa tttattgaca tagaaagtgt
      961 gttgaattag caacagatga cgaaaattaa acggatttat cagaattaaa atgaataaaa
     1021 actactcatt aaattcatca taccgaaccg gaacttgtgg gtcgtagagc ggacatttga
     1081 attcttccag tgtatcggcc agcaaattca ttgttttgcc ttcggtgccg tagcaatcga
     1141 tctggatgct ttgtggcgtg gttttgattt gaaaagcgcc ttttcctttg gtgtttttcc
     1201 aactgttttc atccactttt tcccaagtgg aaaaaaccga attgatacga tttagaatta
     1261 cctgaacagg aagtatttcc aatccttcta cttcttgatt ttcgaccaag gcttcgtaaa
     1321 ccaaatgatg gttgagataa attccgtcct gatattgcca aaaaagtagt tcgtacataa
     1381 ttttattttt ttgccacaga ttgcacagat taacacagat ttaaaaaaaa agagaaatta
     1441 aaaatccgtt tttatccgcg tttttgcttt agcaaatctg cgttcatccg tgttctaaaa
     1501 gccaacgtga tttaatactc aaaaattccg tattcgctgc taatggtcag ttttttggca
     1561 tcggctgctt ctaccctgcc cacgatttgt gcgttgacat tgaacgattt cgaaatggcg
     1621 ataatgtctt gggcaacggc ttcgggaacg taaatttcca tacgatgacc gcagttgaag
     1681 acttggtaca tttctttcca atcggttttg gactgttctt gaatcagttt aaacaatggt
     1741 ggaaccggaa ataaattatc ttttatgatg tgtagatttt gaacgaaatg taaaattttg
     1801 gtttgtgctc ctccactgca atgcaccatt ccgtgaatat ctttggagtc gtatttatcc
     1861 aagatttttt tgatgattgg cgcataggtt ctcgttggcg aaagcactaa ttgtccggca
     1921 tcgattgggg aattttctac cgcatcggtc aattttacct gaccggaata gattagttct
     1981 tccggaacgg cagcatcgta actttctgga tatttggcgg ccaaatattt cccgaaaacg
     2041 tcgtgacgag ccgatgtcaa tccgttgctt cccattccgc cattataact tttctcgtaa
     2101 gtggcctgac cgaaagattc caatcccacg attacgtctc ctgcttttat gttggcattg
     2161 tcaatgactt tagaacgttt catacgagcc gtaaccgttg aatccactat gatggttcga
     2221 acaatatcac ctacatcggc agtttcgcca cctgttgaat gaatggttac tccgaaagaa
     2281 tcgagttctt ttatcagttc ttcggttccg ttgatgatgg ctgaaattac ttcggcagga
     2341 atcaggtttt tgtttcttcc aatggttgaa gaaagtaaaa tattgtcgat ggctcccaca
     2401 cacaataaat cgtcgatatt catgatcagg gcgtcttgcg caataccttt ccaaaccgaa
     2461 atatcaccag tttctttcca atacatatag gccaaagacg atttggttcc tgcgccatct
     2521 gcgtgcataa tgaggcaata gtcttcatcg ttggttaaat aatcagggac aattttgcaa
     2581 aaagcttgag gaaataatcc tttgtcgatg ttttttatgg cgttgtgcac atcttcttta
     2641 gatgctgaaa cccctctttg tgcataacgt ttgctagaat ctgaactcat tttataggtg
     2701 tgtagttgtg gcgcaaatat aattattttt ttgggttttg cctattgggt ttttaacggc
     2761 tttcaggaat tctatagtta atgcccataa aatgaaatta tttcattaaa tttgacatag
     2821 atacctgatt tataattatt taaaataatc aattcacgat aaaaacaact atactagaaa
     2881 agtggaaacc agcgaccact aaaaaaaacg gggcacaacc gaaaagccat agtagtagga
     2941 aagcaaaaat tttaaaagat gcctaagtac gtaattgaaa gagaaattcc cggtgcagga
     3001 aaacttagtc cggaacaatt aaaaggtatt tcacaaactt cctgtggagt attgaacaaa
     3061 atgggatcgc aaatccaatg ggttcacagc tatgtaactg gcgacaaaat ctattgtata
     3121 tacaatgcgc caaacgaaga aatggtttgc gaacacgcca aactaggcgg ctttccggca
     3181 aattccgtaa atgaagttgc aaccatcatt gaccccataa ctgcagaata atttatttaa
     3241 aaaaatcaaa aatgcctctc cagttttgga aaggcatttt tttatggatt aattattgtt
     3301 ttatttggta agcaacaatt ccctgtattt agtcaatgtc cagttttcat catccaccaa
     3361 caattccagt ttgtcgcagt gatttctaat ttcttcgaaa tacggtttta cgttattaca
     3421 ataagcctct gccattgctt gagcattatc taaatgattc gctttttttc tttcgttggt
     3481 catcgcttct actttagaat tgatgccttc gatatgaccg gaaacttccc tgataagaat
     3541 aatttgctct ttggccaatg ttttgaattc atctccgaaa atttctttca aaccttttac
     3601 gttttcaatc aaggtgtttt ggtaacgaat tgcggttgga atcacatgat tttgtgataa
     3661 atctcccaaa accctacctt caatttggat tttcttggtg tattcttcca gttcaatttc
     3721 ataacgagct tcgacttcta cgtgattcat aatgcccatt tctgaaaata aatccaaagc
     3781 ttgttttgat gctctggctt tcaaagcttc gggagtggtt ttatgattgc tcaaccctct
     3841 tttggcagct tctttttccc aagcatcgct gtaaccgtct ccttcaaaaa ggattttttt
     3901 agacaatttg atgtattctc tcaagacatt gaaaattgct tcgtcttttt tcaatccttt
     3961 attatcaatc aaagcatcga cttctacttt aaaatctttc agttgtttag caacaatagt
     4021 attcaaagtg gtcattgcat tggaacaatt cgcagaagac cccacagctc tgaactcgaa
     4081 tttatttcct gtaaaggcaa aaggcgaagt tctatttcta tcggtattat ccaaaatcac
     4141 ttcaggaatt ttacctacca cgttcaattt taaatcggtt ttttcttctg gagataattt
     4201 tccatcggtc acgccttcta attccgccaa aacctttgtt aattgttctc caataaatac
     4261 tgatataatg gctggtggcg cttcattagc acccaatcga tggtcattac tagctgtagc
     4321 aatagatcct cttaataagg attcataatc atttacggct ttgatggtat taataaagaa
     4381 agccaaaaac tgtaaattac tcattggcgt tttgctcgga ctcaacaagt ttacgcccgt
     4441 atctgttgcc aaagaccagt tattatgctt tcctgaaccg ttgacgcctt tgaatggttt
     4501 ttcgtgaaat aatactttaa aatcgtgacg ctcggctact ttttgcatta catccatcaa
     4561 caagcagttg tgatctacag ccagatttgt ttcttcaaaa attggggcta attcaaactg
     4621 atttggagct acctcattat gacgggtttt tacaggaatg ccgagcaaca tacattcttg
     4681 ctctaaatct ctcatataag tcaaagcacg ggttggaatc gagccaaaat aatgatcgtc
     4741 taattgttgt cctttggcag aagtgtgtcc taataaggtt cttcctgtca taatcaaatc
     4801 aggacgggaa tttgctaaag cactgtcaac caagaaatat tcttgttccc aacccaaagt
     4861 ggcggtcact ttttttacat tcttatcgaa atatttacaa acttcggttg ccgcttcatc
     4921 tatggcagat agagctctta acaaaggaat tttattatcc aaagcctctc ctgtataaga
     4981 aatgaaaacc gttggaatac acaaagttgt accataaata aatgctggag atgttggatc
     5041 ccaagccgta tatcctcttg cttcgaaagt atttcggatt ccaccatttg gaaaactaga
     5101 agcgtctggt tcttgctgaa ccaattgtcc gccgccaaat ttttctacag attcgctacc
     5161 atcataagag gtctcgaaaa aagcatcgtg tttttcggca gtcgttcctg taagcggttg
     5221 aaaccagtgt gtataatgag tcacaccttt cgataacgcc cactctttca tccccatagc
     5281 gatgtaatca gctaattttc ggtcaatttt tgttccgttc agaacagcac tttgtactgc
     5341 ttttaaagca tcagaagtca aatattgctt cattgctttt tcattaaaaa cattcgaacc
     5401 aaaaataatt gatttcctgt cagtctcctc aaaatggact ggttttctgc tagaagcttc
     5461 atttaaagct tgaaaacgta atgttgccat aatttcgata atttttaaca aaggtaatta
     5521 ttaattttag catataaaaa attttaattt tacaatatta cccatctaat tatagggtat
     5581 tcacaaaaat tacaacatac atattaattt gccccccttt gttttatact aacaatactt
     5641 ttattttata tttgcatcat aataaaacaa cacttaatta atttatattg ttatggcaaa
     5701 aattaaacta gagtaccttt ggttagacgg atacgaacca actcaaaatc ttagaagtaa
     5761 aactaaagta gaagaacacg aaaactttca aggaacatta gaagaaatag gaaactggtc
     5821 ttttgacggg tcatcaacta gacaagcaga agggggttca tctgactgta tgttggttcc
     5881 tgttgcaatt tacccagatc caactcgcat caacggatat ttagtaatga ctgaagttat
     5941 gaatgctgat ggaactccac atgcatctaa tggtagagct actattgatg atgatggtga
     6001 tttctggttc ggtttcgagc aagagtattt cattatggat actaaaactt tattacctct
     6061 tggtttccct gttggaggtt accctgcacc acaagggatg tactactgtt cagtaggtgg
     6121 aaaaaacaca cacggaagaa aattagttga agagcatgct gatttgtgta tcgctgctgg
     6181 aatcaacttc gaaggaatca atcaagaagt agcttgtgga caatgggaat tccaattgtt
     6241 tgcaaaagga gctaaaaaag caggtgatga aatctgggtt gctcgttact tattggatcg
     6301 tttgactgaa aaatatggtt actatattga ataccaccca aaaccactag gagatactga
     6361 ctggaatggt tctggtatgc acgctaactt ctctaacgaa gttttgagaa cttgtggttc
     6421 taaagaaact tacgataaaa tttgtgaggc tttccgtcct gtaactgaag agcacatcgc
     6481 agtttacgga gcttacaacg agcaacgttt gactggtaaa cacgaaacgg cttctatcca
     6541 cgatttctct tatggagtat ctgatagagg atgttcaatc cgcattccat taatcactgt
     6601 tcaaaaagga tacaaaggat ggttagaaga tagaagacca gcctctaacg gtgatccata
     6661 caaaattgct gcaagaatca tcaaaactgt aaaatcagct ttgtaattct cattcaatca
     6721 taactcaaaa gtgctttcag aaatggaagc acttttttta tacccatttt tgcattattt
     6781 a
//
LOCUS       DAOSNN010000142         6731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11414_length_6731_cov_6.935291, whole genome shotgun sequence.
ACCESSION   DAOSNN010000142 DAOSNN010000000
VERSION     DAOSNN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11414_length_6731_cov_6.935291"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLQ70_07980"
     CDS             complement(<1..380)
                     /locus_tag="PLQ70_07980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQE34590.1"
                     /translation="MKHSFLALIAVVLISCNPKKKEEIATPKIDNETEILKYIADNHL
                     KAKRTESGLYYVIEEAGTGKQPDANSEVTVAYKGYFTNKEVFDQSDVKGISFPLQNVI
                     KGWTEGIPYFKEGGKGILLIPSDLG"
     gene            complement(707..2146)
                     /locus_tag="PLQ70_07985"
     CDS             complement(707..2146)
                     /locus_tag="PLQ70_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQE34591.1"
                     /translation="MVLSRFWLVIFISSIAFVIFSLFSGNSYSIDNVLNGKKDDPILI
                     SEKYIDQIPVFIKDSINKSDEKTFIINRDTLNADTTYVYKNKTVKIFSGVQKSDGLLP
                     TCKATLMDLILPLMAYLAFFCGLMELLIISGASGKLAKMLSPVFVKVFPSVPKDHPSI
                     SYMTLNFAANFLGLDSAATPFGLKAMESLQEINPEKDKASDAQIMFMCLHASGLTLIA
                     TSIIGYRAAANATNPADVMLPCIITSFIGTIAAFLIVGIKQKINFKSASLVVGLITLI
                     AAIIGLLMYVNHLDLIGKNYFTSNLSALILVGIILSTLVFSLLKEKKFTEAKTTVFEA
                     FVQGANNGVKTGVTIFPYVLGMLVAISLFRNSGLFEIISNWIAFIFSNLGVSKGITDA
                     LPVALLRPFSSAGSRGFLIDSMNTFGADSMTGRLSSIFQCSAESTFYVIAVYFGSVNI
                     KNTRYALGTMLLVDVICVITAIFVASWFF"
     gene            complement(2288..3001)
                     /locus_tag="PLQ70_07990"
     CDS             complement(2288..3001)
                     /locus_tag="PLQ70_07990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQE34592.1"
                     /translation="MIEKINLNNILFLDIETVPEVADFNTLDEEMKTLWEHKTQYQRK
                     DEYTPEDFYDRAGIWAEFGKIVCISVGYFVIKSDIRNFRVTSFFGEEPKLLKDFNNLL
                     NNHFNQPQHVLCGHNAKEFDIPFLARRMIINNIAIPDKLNLFGKKPWEIQHLDTLELW
                     KFGDYKHFTSLKLMCKVLGIPSPKGDIDGSQVGHVFYVEKDIDRIVTYCEKDTIAVAQ
                     IFLRLRREELLIEEEIIHV"
     gene            complement(3076..3546)
                     /locus_tag="PLQ70_07995"
     CDS             complement(3076..3546)
                     /locus_tag="PLQ70_07995"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQE34593.1"
                     /translation="METVFVKTPLGIAKIMGDENGVSEISVSDEGVVSNAIPTVLQEA
                     ASQLNDYFEGKRTNFYFKLNPMGTEFQQKVWKALLEIPFGKTMSYLELSKKLGDVKAI
                     RAVASANGKNPLWIVVPCHRVIGTDGSLTGYAGGLWRKKWLLEHENPTTQQRLF"
     gene            3738..4943
                     /locus_tag="PLQ70_08000"
     CDS             3738..4943
                     /locus_tag="PLQ70_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE34594.1"
                     /translation="MTKKLLKNRLFVATALMLVVALAPMQSIAQKKVAYIQFSKVMDA
                     TASQTGDATITGEDAITKMLTATGFVVTVYSCDANGKNLKTNVNVDTEITGADLIIMQ
                     ETWGSTAAAIKPTGILGLNKLSALNIPVIYNKSFAFQKVNTRAITSVAGTTYDVTSGS
                     ALGVQVVAGKESNPMFNGIVGTSIPLIIQGANDLGVVGSGATANVTPYFKGLSLVNDL
                     EISTPGTLLTSLSGSVAAGTPISTTSNDASMVINHIPAGTQIGTVATDKLANKDMITF
                     AVNYGAIAAGKGTNVTNEQLTLWRNAAYILTGQTPPTTLYLNPALGLKENALASDSIS
                     VYPNPTNGLVTVNSTAAVKAITVYDTTGKQISASKTNTVDLSNQAKGVYLVQVQTENG
                     STSKKVVVE"
     gene            complement(5032..5424)
                     /locus_tag="PLQ70_08005"
     CDS             complement(5032..5424)
                     /locus_tag="PLQ70_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQE34595.1"
                     /translation="MKKVIFLLSVLAFVSCKKESEESFGKPETTTETTTETQKPEELG
                     AQIFAGKGACVACHKPDVKLVGPSLQDIAKIYKDKNGDIVSFLKGEGEPIVDPTQYAV
                     MKPNLELTKTFSEEELKALEAYVYSTLK"
     gene            complement(5428..6423)
                     /gene="hemB"
                     /locus_tag="PLQ70_08010"
     CDS             complement(5428..6423)
                     /gene="hemB"
                     /locus_tag="PLQ70_08010"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HQE34596.1"
                     /translation="MFPLQRGRRLRVNESIRSLVRETTLSPSDFMFPMFIAEGENVKV
                     EIPSMVGIYRRSIDLTVEEVKELFALGIRAVNIYVKVDESLKDNTGKEAWNPDGLMQR
                     AIKAIKKACPEMIVMPDVALDPYSIYGHDGIIANGDVENDSTNDALVKMAVSHAQAGA
                     DFVAPSDMMDGRVLRLRQGLDAAGFHNVGIMSYSAKYASAFYGPFRDALDSAPRESDI
                     EVPKDKKTYQMDYANRIEAIKEAVWDVEEGADMVMVKPGIAYLDIVREVKNAVNIPVT
                     VFHVSGEYAMIKAAAERGWLDNDKIMMEQLMCIKRAGASLISTYFAKEAAILLNK"
     gene            complement(6480..>6731)
                     /locus_tag="PLQ70_08015"
     CDS             complement(6480..>6731)
                     /locus_tag="PLQ70_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008611657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HQE34597.1"
                     /translation="EKRKNLVYSDANRTWQEIRRGRYVEFNLVHDKGTLFGLKTNGRI
                     ESILMSLPPHVQWVYDHHPEIGSEEEKLLKVLEKPKNWV"
BASE COUNT         2131 a         1299 c         1132 g         2169 t
ORIGIN      
        1 cctaaatcag atggaatcaa aagaattcct ttgcctcctt ctttaaaata cggaattcct
       61 tccgtccaac ctttgatgac attttgcaac ggaaacgaaa ttcctttgac atcgctttga
      121 tcaaagactt ctttattggt aaaatagcct ttgtatgcaa ccgtaacttc tgaattggca
      181 tcgggttgtt ttccggttcc tgcttcttca attacataat acaaacccga ttcggttcgt
      241 tttgctttta aatgattatc ggcaatgtat tttaggattt ccgtttcatt atcgattttt
      301 ggggtggcaa tttcttcttt tttcttgggg ttacaagaaa ttaaaacaac agctattaga
      361 gctaaaaatg aatgtttcat atgttgatat tgattgtttt tttatcggtc atatgtaatt
      421 cgcatatcaa cattggcggt tgcgaccaat ttattggtta tgctcatttc ataatgtgtt
      481 ttttctgctt ttgattaagt taaaacgctg gtaagcaaga ataaaataac taaagctata
      541 gcaatgtatt tagcatattt tgacattttg tttgtggttt aatgggttat taaatgattt
      601 cgtataaaaa tcaaaagaaa gaaaaataga ttaaaactcc taatattatt tttcaatcaa
      661 cgtatggaat ccgctgattg aaaaaacatt tttattcgtt acatttttaa aaaaaccagc
      721 tcgccacaaa aattgctgta atcacacaaa tcacatctac caaaagcatt gttcccagag
      781 cgtaacgagt atttttgata ttcaccgaac caaagtaaac cgcaatcaca taaaaagtac
      841 tttcagcact gcactgaaaa atgctgctca atcgtcctgt catcgaatcg gcaccaaaag
      901 tattcattga atctatcaaa aatcctcttg atcccgcaga actaaatgga cgaagtaaag
      961 caacaggcaa ggcgtcagta attcctttac tcactcccaa atttgaaaaa atgaaggcta
     1021 tccaattgct gataatttca aacaaaccgc tgtttctaaa taacgaaata gccaccaaca
     1081 tccccaaaac ataagggaaa atggtaacac ccgttttgac tccgttattt gctccttgca
     1141 caaaggcttc gaaaacagta gttttagctt cagtaaattt tttctcttta agaagtgaaa
     1201 acaccaaagt cgaaagaata attcccacca atatcaaagc cgaaagatta gaggtaaaat
     1261 agttttttcc gattaaatcc aaatggttca cgtacatcag caaaccaata atggcagcaa
     1321 ttaatgtaat caagcccaca accaaagagg cgcttttgaa gttgattttt tgtttgattc
     1381 ccacaatcaa gaaagcggca attgtaccga taaatgaagt gataatacaa ggcaacatta
     1441 catcggcggg gttcgtagca ttggcggcag cacgatagcc aataatagaa gttgctatca
     1501 aagtaagtcc cgaagcgtgc aaacacataa acatgatttg cgcatcactc gccttgtctt
     1561 tttcgggatt tatttcttgc aaactttcca ttgctttcaa tccgaaaggt gtggcagccg
     1621 aatccaatcc taagaaattg gcggcaaaat ttaaagtcat atatgaaata gaaggatggt
     1681 cttttggaac gctcggaaat acttttacaa aaacgggact caacatttta gctaatttcc
     1741 cagaggctcc agaaatgatt aaaagctcca tcagtccaca gaaaaaagcc aaatacgcca
     1801 tcaatggcaa aatcaaatcc atcaaagtgg ctttacaagt cggcaacaaa ccatccgatt
     1861 tttgaactcc actgaaaatt tttacggttt tgtttttata aacataagta gtatcggcgt
     1921 ttaaagtatc tctgttgatt ataaaagttt tttcatccga tttattgatg ctgtctttta
     1981 taaaaacagg aatctgatcg atatattttt ctgaaatcag aataggatcg tctttttttc
     2041 cgttcaaaac attgtcaata ctataactgt tccccgaaaa caaactaaaa atcacaaatg
     2101 caatcgacga aatgaaaata accaaccaaa atctacttaa taccataatc tttaattttt
     2161 gtaaatgtaa aaattttaaa aaagtccaac cgcaaattcg caaattaaaa ttaataaacg
     2221 gcaataaaac tataatcatc cgcacataat taccaaaata aaatttgcga atttgcggtc
     2281 atttttttta aacgtggata atttcctctt caatcaacaa ttcctctcgt cgcaaacgta
     2341 gaaaaatttg cgccacggca atggtatctt tttcgcaata ggtcacgatt ctatcaatgt
     2401 ctttttctac ataaaaaacg tgtcctactt gactaccgtc gatatcgcct tttggagaag
     2461 ggattccgag gactttgcac attaatttca aggaagtaaa atgcttgtaa tcgccaaatt
     2521 tccacaattc taatgtgtct aaatgctgaa tttcccaagg ttttttgcca aacaaattca
     2581 atttatcggg aatggcaatg ttgttgatga tcattcgacg tgcaaggaac ggaatatcga
     2641 attccttggc attatggcca cacaaaacgt gttgtggttg gttgaaatga ttgttcaaca
     2701 gattattgaa atccttcaaa agtttgggtt cttctccaaa aaaagatgtc accctaaaat
     2761 ttcgaatatc gcttttaatc acaaaataac ccaccgaaat gcagacaatt ttcccaaatt
     2821 cggcccaaat tccagctcgg tcataaaaat cttcgggcgt gtattcgtct ttgcgctggt
     2881 attgggtttt gtgttcccaa agcgttttca tttcttcatc caaagtattg aaatcggcca
     2941 cttctggaac ggtttcgatg tcgagaaaca aaatgttgtt gaggtttatt ttttctatca
     3001 ttgcaatgta gtttagccac taattcacga atttaattaa ttgtaaggaa aattcgtgaa
     3061 ttagtggcta aaattttaaa acaatctttg ttgcgtagtt gggttttcgt gttccaaaag
     3121 ccattttttg cgccacaatc cgccagcata acccgtgaga gaaccatctg tcccgattac
     3181 acggtgacaa ggaacgacaa tccaaagcgg gtttttccca ttggctgaag ccacagcacg
     3241 aatggctttg acatcgccca atttcttgga taattccaaa tacgacattg tttttccgaa
     3301 aggaatttcg agtagtgctt tccagacttt ttgttgaaat tcagtaccca ttgggtttaa
     3361 tttgaaatag aaattcgttc ttttcccttc gaaataatcg ttgagttggg aagcggcttc
     3421 ttgcaaaacg gttggaattg catttgaaac tactccttca tcagaaacag aaatttcaga
     3481 aacaccattt tcatcgccca taattttggc tattcctagt ggagttttga cgaagactgt
     3541 ttccatagtt ttagaattga attgcaaagt acgcaaagtt ttgatatttc aaacaaagcc
     3601 ttaatttact aatcattaga aaaataatgt aatcgattac atatttatcg tctaaaggct
     3661 tgttttttat attatatttt ttaattttat caaaaaaaaa ttatattcat ttttttaatc
     3721 ctaaaatctt aattattatg acaaaaaaat tactcaaaaa cagattattc gttgctacgg
     3781 cattaatgct agttgtcgct ttagcaccta tgcaatcgat agcacagaaa aaagttgctt
     3841 acattcaatt tagcaaagtt atggatgcta cagcttcaca aacaggtgac gctacaatta
     3901 caggtgaaga tgcgattacc aaaatgctta ctgctacagg atttgtcgtt actgtatatt
     3961 cttgtgatgc taatggtaaa aaccttaaaa caaatgtaaa tgtagatacc gagattacag
     4021 gtgctgacct aatcataatg caggaaactt ggggttcaac ggctgctgct attaaaccaa
     4081 caggaatact tggtcttaat aaattatccg cactaaacat acctgttatt tacaacaaat
     4141 cctttgcatt ccaaaaagta aatactagag ctattacttc tgttgctgga acgacatatg
     4201 atgttactag tggaagtgct ttaggagttc aagtagttgc tggcaaggaa tctaacccga
     4261 tgtttaacgg aatagtaggc acaagtattc ctcttataat tcaaggagct aacgatttag
     4321 gagtagtagg tagtggtgct actgctaatg ttacccctta ttttaaaggt ctttctctag
     4381 taaatgattt agaaatttct acccctggaa ctttattaac gtctctttct ggatctgttg
     4441 cagctggaac ccctatatca actacttcta atgatgcttc aatggtaatt aatcacatac
     4501 ctgcaggaac acaaatagga acagttgcta ctgataaatt agccaacaaa gatatgatta
     4561 cttttgccgt aaattatgga gcaattgctg ctggaaaagg aacaaatgtt acaaatgaac
     4621 aattaacact ttggagaaat gcagcttata tattgacagg acaaacacct ccaactactt
     4681 tgtatttgaa tcctgcttta ggattaaaag aaaacgcttt ggcatcagat agtatttcag
     4741 tatatccaaa ccctactaat ggtttagtta ctgtgaatag cacagcagct gtaaaagcaa
     4801 ttactgttta tgatactaca ggaaaacaaa tttctgcaag caaaaccaat acagttgatt
     4861 tgtctaacca agctaaagga gtttacttgg tacaagttca aacagaaaat ggttctactt
     4921 ccaaaaaagt tgtggtagaa taaatcacta ataattttaa aataagaaag aggctgtttc
     4981 gcaagttagt gaaacagcct cttttttttg ttgcaaaaac actgatgggt attatttcag
     5041 agtcgaataa acataagctt ctaaagcctt caattcttcc tccgaaaaag tcttggtgag
     5101 ttctaaattg ggtttcatta ctgcgtattg ggttggatca acaattggtt ctccttcgcc
     5161 ctttagaaat gaaactatat ctccgttttt gtctttatag attttggcaa tatcttgcaa
     5221 actgggtcca acgagtttta catcgggttt gtgacaagct acgcaagctc cttttccagc
     5281 aaaaatttgc gctcctaact cttctggttt ttgagtttcg gtggtggttt cagttgtggt
     5341 ttcgggtttt ccaaaggatt cttcgctctc ttttttacaa gatacaaatg ccaatacgga
     5401 taataaaaat ataactttct tcattgatta tttatttaaa agtattgcag cttctttggc
     5461 aaaataagtt gaaatcaggc ttgctcctgc ccttttgata cacattaatt gttccatcat
     5521 aatcttgtcg ttgtcgagcc aacctctttc ggcggcagct tttatcattg cgtattcacc
     5581 ggatacgtga aaaaccgtta cgggaatatt cacagcattt tttacttcac gaacgatatc
     5641 caaataagcg attccaggtt ttaccatcac catatcggca ccttcttcta cgtcccaaac
     5701 ggcttctttg atggcttcga ttcgattagc ataatccatt tggtaggttt tcttgtcttt
     5761 tggaacttca atatcagatt ctcttggcgc actgtccaaa gcatcacgga aaggcccgta
     5821 aaatgccgaa gcatatttag ccgaataact cataatcccc acgttgtgga aacctgccgc
     5881 atccaaacct tgtcgcaaac gcaaaactcg accgtccatc atatcggaag gagcaacaaa
     5941 atcggctcca gcctgagcgt gggaaacagc catttttacc aaagcatcgt tggtggagtc
     6001 attttctaca tcgccattag caataatacc gtcgtggcca taaatagaat acgggtctaa
     6061 cgccacatcg ggcattacta tcatttctgg acacgctttt ttaatggctt taatagccct
     6121 ttgcattaat ccatcaggat tccaagcttc ttttcctgta ttgtccttta gggattcatc
     6181 tacttttaca taaatgttta cagcacgaat tcctaaagca aaaagttctt tgacttcttc
     6241 tacagttaaa tctatcgaac gacggtaaat gccaaccatc gaagggattt caacttttac
     6301 gttttcgcct tcagcaataa acatcggaaa cataaaatcg gatggactta atgttgtttc
     6361 acgaactaag gaacgaatgc tttcgttcac gcgtaatctt ctgcctcttt gtaatgggaa
     6421 catactattt tgtttaaatt gttttaaagt ttaagtttta aagttttttt actttgtact
     6481 taaacccaat tttttggttt ttctaatact ttcaataact tttcttcttc gcttcctatt
     6541 tcaggatgat ggtcatacac ccactgaacg tgtggtggca aactcatcag gatactttca
     6601 attctaccgt tggtttttaa gccaaataaa gtgcctttgt cgtgaactaa attgaactcc
     6661 acataacgac cacgacggat ttcctgccaa gttctattgg catccgaata tactaaattt
     6721 tttctttttt c
//
LOCUS       DAOSNN010000143         6706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11465_length_6706_cov_4.980304, whole genome shotgun sequence.
ACCESSION   DAOSNN010000143 DAOSNN010000000
VERSION     DAOSNN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11465_length_6706_cov_4.980304"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PLQ70_08020"
     CDS             complement(<1..693)
                     /locus_tag="PLQ70_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HQE34598.1"
                     /translation="MLFNSISFLVFLPIVFILYWWSNKKSLPLQNVLLLGASYFFYAC
                     WDWRFLFLLLFSTALDYFTGIKMAEAKNQGHKKVWFWLSIIVNLGFLGVFKYYNFFAT
                     SFTELLAGVGIQANFWTLKVILPVGISFYTFHGLSYVIDIYKGRIQPERNGVTYSLFV
                     AYFPLLVAGPIERATHLLPQIKKKRVFDYSKAIDGVHQIIWGLFKKVVIADNCALFAN
                     DIFDHYQSMNSLS"
     gene            complement(693..1814)
                     /gene="neuC"
                     /locus_tag="PLQ70_08025"
     CDS             complement(693..1814)
                     /gene="neuC"
                     /locus_tag="PLQ70_08025"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HQE34599.1"
                     /translation="MKKILFLTGTRADFGKIKSLIQILDDHQDFEVFVFVTGMHLQKE
                     YGYTLIEIERCQFKNVHTFENHTHETTMDLTLAKTIEGLSAYVKEINPDLIVVHGDRV
                     ETLAGAIVGSLNNILVAHIEGGELSGTVDELIRHSVSKLSHIHFVSNAEAAKRLKQMG
                     EIESSIFTIGSPDIDIMFSDDLPELSIVKEYYAIPFEKFAVVMFHPVTTEVKQMQQYA
                     ENFVKSLVADSHNYVVIFPNNDLGSQFIIEEYQKLKDNPRFRIFPSLRFEYFLTLLKS
                     SQFIIGNSSAGIREAPYYGIPIINIGTRQQNRAVHADIINVGYELESIQEALMVIDTH
                     KIQMSDADYGKGNSTELFLECLQKSNVWKLNHQKQFRDT"
     gene            complement(1816..2856)
                     /gene="neuB"
                     /locus_tag="PLQ70_08030"
     CDS             complement(1816..2856)
                     /gene="neuB"
                     /locus_tag="PLQ70_08030"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HQE34600.1"
                     /translation="MNPYIEIAGRKIGLDYPPLVIAEIGINHEGSLQVAKEMVDAAKR
                     AGVELVKHQTHIVEDEMSGAAKKVIPGNADVSIYEIMERCSLNEAEELELKNYVESKG
                     MIFISTPFSRAAAERLKKFDIPAYKIGSGECNNYPLLEHIASFGKPVILSTGMNTIES
                     IQKAVAIFDKHNIPVALLHTTNLYPTPIHLVRLGAMMEMHNAFPDKVFGLSDHTLKNN
                     ACLGAVALGASILERHFTDTMQRTGPDIVCSMDEQATKELIISSAEIALMRGGTKKPA
                     LEEQVTIDFAFATVCAIKNIKKGEVFTKENIWVKRPGTGKILAEHFNDVIGTIATRAI
                     MNDEQLDFSDFE"
     gene            complement(2865..3539)
                     /locus_tag="PLQ70_08035"
     CDS             complement(2865..3539)
                     /locus_tag="PLQ70_08035"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HQE34601.1"
                     /translation="MKTIAIIPARGGSKRLSNKNVLSLGGIPLIAHSILYAQKNSDII
                     DEIYVSTDDAQIKEVALQYGAKVIDRSEHLSGDFEPTVSALKHVLESIEINVESVILL
                     QATNPLRPQNLLAAAFESYEKGNYDSLFTVSRNHQKLGKIVENKFEPFNYTIGQRSQD
                     LEPLFYENGLLYITKANLIFNDSIISENAYPFELNSIFANVDIDTQDDLELAEYLYQK
                     FITHNS"
     gene            4007..4390
                     /locus_tag="PLQ70_08040"
     CDS             4007..4390
                     /locus_tag="PLQ70_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQE34602.1"
                     /translation="MAIKYKVLPRKNPRDIAAPEKFYAAAIADGDVDLDRLAELISYQ
                     CTVTATDCYAVLHSLEHNIIGELEQGRIVKLGRLGNFQVGISSEGKDTATEVTTTNIV
                     KSRILFRPGKRLRSMLNGLTFKKAG"
     gene            complement(5074..5982)
                     /locus_tag="PLQ70_08045"
     CDS             complement(5074..5982)
                     /locus_tag="PLQ70_08045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE34603.1"
                     /translation="MSNPTFAILITTKNRKTDLAFTLNKIKCLLKESKVACVVFDDGS
                     TDGTFEYVKENFPEVQLQRNPVSKGYIFCRNKMLNETQADYAISLDDDAHFVTENPLE
                     FIRNHFEANANCGLIALRIFWGLQEPKSTISVVKSQRVQGFVGCAHVWRMSAWRKIPN
                     YPEWFVFYGEENFASFQLFKTNSEIHYLPEVLVHHRVDVKARRNDADYTIRLRRSLRS
                     GWYLFFLFYPLKTIPRKMAYSLWMQLKMKVFKGDFKALKAIVLALFDLIWNSSKIIKN
                     SNRLTQKEYESYNQLPETQLYWQPEK"
     gene            complement(5993..>6706)
                     /locus_tag="PLQ70_08050"
     CDS             complement(5993..>6706)
                     /locus_tag="PLQ70_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34604.1"
                     /translation="VSQSKTCMSSAQLMAEDMQYLYGSRKKFYHLPHQYDASVFDLSD
                     VSTVLKKNRKILISYHGAIMFGRNIEILLDTYIDLIENNAVYKENTEFVLRLKGSDLK
                     KMTEKYSGTPNIQVLGMVSFSNSCFEQSHEADINIILENGPIYSNTLVGKAPFLAATG
                     KPVFVLAPERSEMRSLLKDNQYIASYSDAAEIKSKLENLIQNRLNSDEAVYPFEDYFS
                     DENFKIHLDAILFAKGGEN"
BASE COUNT         2211 a         1275 c         1084 g         2136 t
ORIGIN      
        1 cgacaaagaa ttcatcgatt ggtaatggtc aaaaatgtcg ttagcaaata aagcgcaatt
       61 atcggcgatg actacttttt taaataatcc ccaaattatt tgatgcactc catcaatggc
      121 tttactgtaa tcgaaaactc gttttttctt tatttgtggt aataaatgag tcgccctttc
      181 tatggggcca gctaccaaca gcggaaaata agctacaaat agcgaatagg taacaccatt
      241 tctttcgggt tggattcttc ctttataaat atcaatcaca tacgacagac cgtgaaaagt
      301 ataaaaggaa attccaactg gcaaaatgac tttcaaagtc caaaaattag cttgtattcc
      361 aactcccgca agcagttcag taaaagaagt agcaaagaaa ttataatatt taaatacccc
      421 taaaaaacca agattcacga taatactcaa ccaaaaccaa acctttttat gaccttgatt
      481 tttggcttcc gccattttta ttcccgtaaa ataatccaaa gctgtagaaa aaagcaacag
      541 aaaaagaaaa cgccaatccc aacaggcata aaaaaaataa ctcgcaccaa gcaacaatac
      601 attttgcagt ggcaagctct ttttattgct ccaccaataa aggataaaaa caatgggaag
      661 aaatactaaa aaggaaatgg aattaaaaag cattaggtat ctctaaattg tttttgatga
      721 ttgagcttcc aaacattcga tttttgaaga cattcgagaa acaattcagt actatttcct
      781 tttccataat ctgcatccga catttgaatt ttatgggtgt cgataaccat caaagcctct
      841 tgaatcgact ctaattcgta accaacatta ataatatcgg cgtgtactgc cctattttgc
      901 tgtctggttc caatattgat gattggaatg ccataataag gggcctctcg aatacccgca
      961 ctactgttgc ctatgatgaa ctgactgctc ttcaacaaag tcaaaaaata ttcaaaccgt
     1021 agggacggaa aaattcggaa tcgaggatta tccttcaatt tttgatattc ttcaataata
     1081 aattggcttc ccaaatcatt gttgggaaaa ataaccacat aattatgact atcggctact
     1141 aacgatttta caaaattttc agcatactgt tgcatctgct tcacctcggt agtaacagga
     1201 tgaaacatca caacggcaaa tttctcaaaa ggaatagcat aatattcttt tacaatagac
     1261 aactctggca aatcatctga gaacataata tcaatgtctg gcgaaccaat cgtaaaaata
     1321 gaagattcta tttcccccat ttgtttgagt cgtttggctg cttcagcatt cgaaacaaaa
     1381 tgaatgtgac tcaatttact aacactatgc cgaatcaact catcaacagt tcccgacaat
     1441 tctcctcctt caatatgcgc taccaaaata ttatttaaag aacctacaat tgcaccagcc
     1501 aaggtttcta ctcgatcacc gtgtactaca attaaatccg gattgatttc tttgacataa
     1561 gccgaaagtc cttcaatggt tttggccaat gtcaaatcca ttgtggtttc gtgagtatga
     1621 ttttcaaaag tgtgcacgtt tttaaactga catctttcaa tttcaatcag cgtataacca
     1681 tactcctttt gcaagtgcat tcctgttaca aaaacaaaaa cctcaaaatc ttgatggtca
     1741 tcaagaattt gaatcaatga ttttattttg ccaaaatcgg ctcgagttcc tgttaggaaa
     1801 aggatttttt tcattttact caaaatcaga aaaatctaat tgttcatcat tcataatagc
     1861 tctggtggcg attgttccga taacatcatt aaaatgttcc gctaaaatct ttcctgttcc
     1921 gggacgtttg acccaaatat tttctttggt aaacacttct ccttttttta tgttttttat
     1981 cgcacaaact gtcgcaaagg caaaatcaat ggttacttgt tcttcaaggg cgggtttttt
     2041 cgttccgcca cgcatcaaag ctatttcggc cgaagaaata attaattctt ttgtagcctg
     2101 ttcatccata ctacacacaa tatcggggcc tgtgcgctgc atggtgtctg taaaatggcg
     2161 ttctaaaatg cttgctccca aagccactgc tcccaaacaa gcattgtttt ttaaagtatg
     2221 atctgaaagt ccaaaaactt tatctggaaa ggcattgtgc atttccatca tcgcacccaa
     2281 tcgtaccaaa tgaatgggag tgggatataa attggtcgtg tgcaaaagtg ccaccggaat
     2341 gttgtgcttg tcaaaaatcg ctactgcttt ctgaatactt tcaatggtat tcataccagt
     2401 acttaaaatt acgggtttgc caaaggaagc tatatgttct aataaaggat aattattgca
     2461 ttcgcctgaa ccaattttat aagctggaat gtcaaatttt ttcaatcttt cggcagcagc
     2521 acgggaaaaa ggcgttgaaa tgaagatcat ccctttgctc tcgacatagt ttttcagttc
     2581 taattcctcg gcttcgttga gcgaacaacg ttccataatt tcataaatag aaacatcggc
     2641 attgcctgga attactttct tggcagcacc actcatttca tcttctacaa tatgggtttg
     2701 atgcttaacc aattcaactc cagctctttt tgcagcatcc accatttctt tggccacttg
     2761 caaagagcct tcgtggttga tgcctatttc ggcaatgact aatggcggat aatctaaacc
     2821 tatttttctt cctgctattt ctatgtatgg attcattaaa tgagttatga gttatgagtt
     2881 atgaattttt ggtaaagata ttctgccaac tccaaatcgt cttgggtatc aatatctaca
     2941 ttggcaaaaa tagaatttag ctcgaaagga taagcgtttt ctgaaataat actatcatta
     3001 aaaatcaaat tggctttggt aatatacagc aagccgtttt cataaaacaa aggttctaaa
     3061 tcttggctac gttgtccaat ggtgtaatta aaaggttcaa acttattttc aacaattttc
     3121 cccaactttt gatggtttct ggaaacggta aacaaactgt cataatttcc tttttcatag
     3181 ctttcaaaag ctgctgccaa taaattttgc ggccgcaatg gattagtcgc ttgcaacaaa
     3241 ataacactct ctacattgat ttcaattgat tccaaaacgt gttttaaagc cgaaacggtc
     3301 ggttcaaaat caccagataa atgctcagat ctatcaatta ctttggcgcc atattgtaaa
     3361 gcaacctctt tgatttgcgc atcatcggtc gaaacataaa tttcgtcaat aatgtcactg
     3421 tttttttggg catataaaat actatgagct attaagggaa tcccacccaa agatagaaca
     3481 ttcttgttgg acaaccgttt tgaaccgcca cgggctggaa tgatggctat tgttttcatt
     3541 tcaattttta ttgatggtaa aaatacttat ttttatagat atcgatgaag tatttagttg
     3601 aaattgagtg tcaaatttgc aaattaacta gtttcaattt tgacaaaatt ttagactaac
     3661 tcgctttttt atgattaaca ctgtgttaag aagaaaatta taaaaaaatt aaaatatgac
     3721 atacatcatt gttttcaaaa taaaaagttt tctgtttttt cacctcgtca agacgaaata
     3781 aaagactaga taataacatt ttgaaaatca ataatttaaa cagactaaga aagtagctga
     3841 caaaaaaacc tgtaatctta catttgttaa aaactcaatc caattgtgaa taagtttgac
     3901 tttttattac aaaaaaacca ttgtatgttt gtaaatgtca gttagatacg aatgacaggt
     3961 atactaagac aaaaattatt atttttaatt taataccata aaaaccatgg ctattaagta
     4021 caaagttctt cccagaaaga accctcggga catcgctgct cccgaaaaat tttatgccgc
     4081 tgccattgca gacggtgatg tcgatttgga tagactcgca gagttgattt cctaccaatg
     4141 tacagtcact gctactgact gttatgctgt tttgcactca ttagagcata acatcattgg
     4201 agaattggag caaggtcgaa ttgttaaact aggcaggcta ggtaattttc aggtgggcat
     4261 aagctcagaa ggaaaagata ctgcaaccga agttacaacg actaacatcg taaagagtcg
     4321 tattcttttc cgaccaggaa aaagattacg ctctatgttg aatggtttaa cattcaaaaa
     4381 agcagggtag gaccccgtaa tcaggagtaa ctgattgttg taagacctcg agtaattaca
     4441 gttactcggg gtcttttttt tggtttatca gcaaaaacac ccttcatttc gtcttgacaa
     4501 aacttttatc ccgtcttgac gaggctttta tcccgtcttg accgtatgat tatttcgtct
     4561 tgacagtcta taaatgaatt aaaaacactt aaaaaacgat gttttttgag catcaaaaga
     4621 caatattagc ttaaaataaa tggtttcaac tgccattaaa gcctttattt gcccaaaaat
     4681 tcttaaataa tcaaccttta tttttccttt aaatgcataa aaaaagctca aaaaatactg
     4741 gtaggacccc gttttcttga gcaaattaat tattgtaaga cctcgagcaa ttacagttac
     4801 ccgttaacaa tggttaaaaa taaaattaat tttcattatg aacaaatttt gttcataatc
     4861 tgttaaaagt ttttattgtt tttatgaagc cacagcatta acctagtttc ccacacctaa
     4921 gtaaaaaatc gctttcatca gctacccaaa actaattaaa gcagacgttt tacaacgtgc
     4981 caaaaggagt cgtcagattg gaaaaatata ataatttggg gagtgcaaat tgaaattatg
     5041 atttacaatt tgcagggttt ttaaagagtt ttactatttt tcaggctgcc aatacaattg
     5101 ggtttctgga agttgattgt agctttcgta ctctttttgg gtcaaacggt tgctgttttt
     5161 tataattttg gaggaattcc aaatcaaatc gaacaaagcc aaaacaatag ctttcaaagc
     5221 cttgaaatcg cctttgaata ctttcatttt caattgcatc caaagcgaat acgccatttt
     5281 tcgaggaatg gtttttagag gataaaacaa gaaaaataaa taccagccgg aacgcaaaga
     5341 ccgtcttaac cgaatggtgt aatcagcatc atttcttctg gctttgacat cgactctgtg
     5401 atgaaccaaa acttcaggta aataatggat ttctgaattc gttttaaaca attgaaagga
     5461 agcaaaattc tcttcgccat aaaaaacaaa ccattcagga taattaggaa tcttgcgcca
     5521 agcgctcatt ctccatacgt gtgcacaacc tacaaaaccc tgaactcttt gtgattttac
     5581 aacagaaatt gttgatttag gttcttgcaa tccccaaaaa atacgcaagg ctatcaaacc
     5641 acaattcgca ttcgcttcaa aatggttgcg gatgaattct aatggatttt cggtaacaaa
     5701 atgggcatcg tcgtctagtg aaatagcata atcggcttgg gtttcattca gcattttatt
     5761 cctacaaaaa atataacctt tagaaacagg atttctttga agttgaactt caggaaaatt
     5821 ttctttcaca tattcaaaag tcccatcggt ggagccatca tcaaaaacca cacatgctac
     5881 tttgctttct ttcagtaaac acttgatttt attcaaagtg aaagccaaat cggttttgcg
     5941 atttttggtg gtgataagga tggcgaaagt gggatttgac atattactaa tattaatttt
     6001 cccctccttt ggcaaacaga atagcatcca aatgaatttt aaaattttca tccgaaaaat
     6061 aatcctcaaa cggataaact gcttcgtcag aatttaacct attttggatt aagttttcta
     6121 gtttactttt gatttctgct gcatccgaat agctggcaat atattgattg tcttttagta
     6181 aggaacgcat ttcacttcgc tctggagcca aaacaaatac aggcttacca gtagcagcta
     6241 agaaaggtgc ttttccaacc aaagtattac tataaatagg tccgttttct aaaatgatat
     6301 tgatatcagc ttcatgactt tgttcaaaac aggaattgga aaaactcacc attcccaaca
     6361 cttgaatatt gggagttcca ctgtattttt cagtcatttt tttcaaatca gatcctttta
     6421 atcgcaagac aaactctgtg ttttctttat aaacagcatt gttttcgatc aaatcaatat
     6481 aagtgtccaa aagaatttct atgttcctgc caaacataat agctccatga taggaaatca
     6541 atatttttct gttttttttc aaaacagtac tcacatccga caaatcaaaa acactagcat
     6601 cgtattgatg cggcaaatgg taaaactttt ttctcgagcc atacaaatac tgcatatcct
     6661 cggccattaa ctgagctgaa ctcatacagg ttttggactg agaaac
//
LOCUS       DAOSNN010000144         6651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11601_length_6651_cov_5.027441, whole genome shotgun sequence.
ACCESSION   DAOSNN010000144 DAOSNN010000000
VERSION     DAOSNN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11601_length_6651_cov_5.027441"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..756
                     /locus_tag="PLQ70_08055"
     CDS             151..756
                     /locus_tag="PLQ70_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQE34605.1"
                     /translation="MKKLASLLVLTAVFVVSGFATDVPTPYKIGDKATDFRLKSVDGK
                     MYSMADYKDAKGFIVVFTCNHCPFAVKYEDRINDLSKKYKSKGYILLAINPNDPALEP
                     TDSYELMKVRAKEKGFAFPYLFDDGQKIYPQYGATKTPHVFLLDKNQIVKYMGAIDDN
                     VDSAADVKEKYLENAIAALESGRTPSPETTKAIGCSIKTKK"
     gene            complement(812..1582)
                     /locus_tag="PLQ70_08060"
     CDS             complement(812..1582)
                     /locus_tag="PLQ70_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020080492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQE34606.1"
                     /translation="MSKLAAKDKFLDLSDYGRPLAKLFANGLKNTQFTPIQVTLAFGV
                     CGLIAIYCIIQNHYFLASFFIILKSIIDAVDGELARIKNTPSYVGRYLDSVFDIILNF
                     LFFGTICFVSKTSLGMTLIAFFCIQLQGTLYNYYYVILRHNSAGGDKTSKIFEDQSPQ
                     ALPGETQKAVDILFKIYTLVYGLFDKIIHVLDSDAYKVKTIPNWFMTFISFFGLGFQL
                     LIIAIMLSLGWIEMIVPFFISYSILIFVFIGIRKVFIK"
     gene            complement(1640..2092)
                     /locus_tag="PLQ70_08065"
     CDS             complement(1640..2092)
                     /locus_tag="PLQ70_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34607.1"
                     /translation="MNPLIVVDAQKIGFMEDHKDLMEKMNPNDISSLTVYKDSLVCKK
                     YGSNSGVIVITNKKFILDTFYKNNIENSPLKGKIKTPEDLLRIGVINDNPKSKNQPYD
                     ELYKYIYTYNINEKIKKVAKITFINPEESIKINSEWINGAIEIDAAIE"
     gene            complement(2173..3195)
                     /locus_tag="PLQ70_08070"
     CDS             complement(2173..3195)
                     /locus_tag="PLQ70_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQE34608.1"
                     /translation="MKNKNKSVIIWLLSGCFLVFVMVVVGGITRLTNSGLSMTDWHLV
                     TDTFPPLTEAKWQETFERYKQFPEYKKINIHNNFTLDDYKFIYFWEWFHRFIGRIIGM
                     VFLIPFIYFLIKKRLSSETIKKCVVLLGMGALQGFLGWFMVRSGLIDNPDVSHFRLSL
                     HLTFAFITFAYTLWVALDLIYPERNTPVLPLRNIARYALVFLLIQIIYGGFVAGLNAG
                     LIHNHWPLMSDGQFLHESVLLEKDSWLLRLTEGKSGVQFVHRTMAYIVVGMILFLAFK
                     SHKFDLTKDQKSGITALVVMVFLQFGLGVFTLLYSVPLWLGLTHQIMAFFLLTAMTYT
                     LHRLAK"
     gene            complement(3275..4693)
                     /locus_tag="PLQ70_08075"
     CDS             complement(3275..4693)
                     /locus_tag="PLQ70_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQE34609.1"
                     /translation="MNYKQALNNKIFEVISQASQELNVDSYVIGGFVRDLLLGRDFKK
                     DIDVVAVGSGIELALKVSELLPNKPKVQVFKTYGTAMLRFEDTEIEFVGARKESYTHD
                     SRNPVVENGTLEDDQNRRDFTINALALSLNENNFGNLSDPFNGLEDMKNKNIKTPLDP
                     DITFSDDPLRMLRGIRFATQLGFEIEANSLNSITKNAERIKIISGERIVDELNKILST
                     DKPSVGFLLLYKTGLLDIILPELTALNQVEEIEGQTHKNNFYHTLEVVDNICPNTDDV
                     WLRWAALLHDIGKAPTKRFNKKQGWTFHGHEFLGGKMAKKIFERLRMPLNHKMKFVQK
                     MVMMSSRPIVLSQDLVTDSAVRRLVFDAGEDVESLMTLCEADITTKNPNKFKKYHQNF
                     EIVRKKIVEVEERDSIRNFQPPISGEEIMAIFNLQPSREIGILKEAVKEAILEGEIPN
                     EYQAAFDFVLKRGAKLGLKKID"
     gene            complement(4755..5141)
                     /locus_tag="PLQ70_08080"
     CDS             complement(4755..5141)
                     /locus_tag="PLQ70_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HQE34610.1"
                     /translation="MEDEYLYKDESYKIVGILLEVHKHLGKGFSEIVYKDAIEYEFQE
                     QNIPYQREKEFSINYKDTILKHKFYADFVVYDKIILEIKTVDCFNNSHYNQSINYLKV
                     SKNKLAILANFNLVSLEYKRIIDSKK"
     gene            complement(5248..5817)
                     /locus_tag="PLQ70_08085"
     CDS             complement(5248..5817)
                     /locus_tag="PLQ70_08085"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQE34611.1"
                     /translation="MENLNEEIHKAFEIIQQGGIILYPTDTVWGIGCDATNAEAVAKI
                     YKLKQRDDSKSMICLMNGEKMMYNVFKDIPEVAWQIIDLSEKPTTLILDNPRNVAANL
                     IATDKTLGIRIVKEPFCFKLMERMKKPLVSTSANISGLPTPIAFKDISPEIIKGVDYV
                     VNLYQDKINGKPSTIIKLTSDSQVKVIRK"
     gene            5998..>6651
                     /locus_tag="PLQ70_08090"
     CDS             5998..>6651
                     /locus_tag="PLQ70_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HQE34612.1"
                     /translation="MILKIDSHFEKYASSLLQIVENFKTSGLLFGDGKRNTIKLFEFE
                     DLTVNIKSFKIPHLFNRVVYQYFRKSKARRSYEYANALLKKGIGTPQPIAYFENSSWL
                     GLKDSYYVSEHLEVDLTFRELDIQPDYPDHENILRQFTRFSFDLHEKGIEFLDHSPGN
                     TLIKNGINGNYSFYLVDLNRMNFDKSLDFEKRMKNLSHLTAKKEMIRVMSDEYAKLSG
                     "
BASE COUNT         2166 a         1107 c         1130 g         2248 t
ORIGIN      
        1 ttccacaggt cgataaagag tggtcaggat ccattccggc aacattaatt ataaaaaatg
       61 ggcagcaatt ttttgccgaa aaaagctttt caagttatgc cgaactcaat caatatgtaa
      121 ataaatttac gaattaaatc actttttagt atgaaaaaat tagcttcact tttagtatta
      181 actgcagttt ttgttgtgtc aggttttgca actgatgtac caacacccta caaaattggc
      241 gataaagcca ccgattttag attaaaatcc gttgacggaa agatgtatag tatggctgat
      301 tacaaagacg caaaaggctt tatagttgtt tttacttgca atcattgtcc ttttgccgtt
      361 aagtatgaag atcgaattaa tgatttgtcc aaaaaataca agtcaaaagg atatattttg
      421 ttagcgatta atcccaatga tcccgcttta gagcctactg atagttatga attgatgaaa
      481 gttagagcca aagaaaaagg gtttgctttc ccgtatttat tcgatgacgg tcaaaaaata
      541 tatccacaat atggcgctac caagacgcca cacgtgtttt tattagataa aaatcagatt
      601 gtaaaataca tgggagctat tgatgacaac gtggatagtg ctgctgatgt aaaggaaaaa
      661 taccttgaaa atgccattgc agctttagaa agcggtagaa caccttcgcc agaaactaca
      721 aaagctatag gttgttcgat taaaaccaag aaataattta tctcaattaa atttataaaa
      781 agagtaaaaa taagttcgtt tttactcttt tttattttat aaataccttt cttattccta
      841 taaaaacaaa gattagaatg gagtaggaaa tgaaaaatgg aactatcatt tctatccatc
      901 ccaaagaaag cataatagca ataattaaca attgaaaacc taatccaaaa aaagaaatga
      961 aggtcataaa ccagtttggg atcgttttta ctttataagc atcgctatct aaaacgtgaa
     1021 ttattttgtc gaaaagaccg taaacgaggg tgtaaatttt aaacaatata tcaactgctt
     1081 tttgggtttc tccaggcaat gcttgaggtg attggtcttc aaaaatttta ctcgttttgt
     1141 cgcctcctgc tgaattgtgt cttaaaatca catagtaata attgtataaa gtgccttgca
     1201 attgaatgca aaaaaaggca attagtgtca ttcctaatga tgtttttgaa acgaaacaaa
     1261 ttgttccaaa aaacaaaaaa ttcagaataa tgtcaaaaac actgtccaag tatcttccaa
     1321 cataagaagg cgtgttttta attctggcta attcgccatc gacagcatca ataatagatt
     1381 tcaagatgat aaaaaagctg gctaaaaaat agtgattttg tataatgcag taaatggcaa
     1441 tcaagccaca aactccaaaa gccaatgtga cctgaattgg tgtgaattgg gtatttttta
     1501 acccattggc aaacagtttt gcaagaggtc ttccgtaatc ggataaatcc aagaatttat
     1561 ctttggcagc aagtttagac atttatagat gttaaacaaa aggatgcgac aatatagtca
     1621 gtagtaaaaa tagtgaaaat tattcgattg cagcatcaat ttcaatagct ccattaatcc
     1681 attctgagtt tattttgata gattcttctg gatttataaa agtgattttg gcgactttct
     1741 ttattttttc gttaatgtta taagtataaa tgtatttgta caattcatca taaggttggt
     1801 ttttgctctt tggattgtca ttgataactc caattctcag caaatcttct ggtgttttta
     1861 tttttccttt taaaggagaa ttttcgatgt tatttttata aaaagtatcc aatatgaatt
     1921 ttttgttcgt tatgacaatc actcctgaat tcgatccata ttttttgcat actagcgaat
     1981 ctttataaac ggttaaagag gaaatgtcat ttggattcat cttctccata aggtctttat
     2041 gatcttccat aaaacctatt ttttgggcat caacaacaat caatggattc atttttgatt
     2101 gtgcaaaaag acttgatacg aataatatgg agaataagaa caatagtttt ttcataattt
     2161 tattttaaac cgttatttcg ccaagcgatg taaagtataa gtcatagccg tcaacaagaa
     2221 gaaagccatt atttgatgtg tcaatcccaa ccaaagcgga acgctgtata gcaaagtaaa
     2281 aacacctaaa ccgaattgta aaaacaccat aactaccaga gcggtgattc ctgatttttg
     2341 gtcttttgtt aagtcgaatt tatgactttt aaaagctaaa aagagtatca ttcccactac
     2401 aatgtatgcc attgttcggt gaacaaactg aacgccactt ttgccttcgg tcaatcttaa
     2461 taaccaagaa tctttttcta atagaacact ttcgtgaaga aactgcccat cactcatcaa
     2521 tggccaatgg ttgtgaatca agccagcatt taaacccgct acgaaaccac cataaataat
     2581 ttgaattaac agaaaaacca aagcatatcg agcaatattt cttagtggta aaacaggagt
     2641 gtttctttct ggataaatca aatccaaggc cacccaaagc gtataagcaa aagtgataaa
     2701 tgcaaaagtc aggtgtaagg aaagtctaaa atggctcaca tctggattgt caattagtcc
     2761 gctgcgaacc ataaaccaac ctaaaaaacc ttgcaatgca cccattccga gaaggaccac
     2821 acattttttg atggtttcag atgaaagccg ttttttgatt agaaaataga tgaaaggaat
     2881 taggaatacc attccaataa ttcttccgat aaaacggtga aaccattccc agaaatagat
     2941 gaatttataa tcatcaagtg taaaattatt gtgaatattt atttttttgt attccggaaa
     3001 ttgcttgtag cgctcgaatg tttcttgcca tttggcttct gtcaaaggag ggaaagtatc
     3061 ggttaccaaa tgccagtctg tcattgaaag tcccgaatta gtcaaacggg taatcccgcc
     3121 aaccacaacc attacaaaca ctaagaaaca acctgaaagt agccagatta ttacggattt
     3181 atttttattt ttcatttctt tctaaaaata ttttgcaaaa ttattgataa gcttatgaat
     3241 tccaacttca ataaaagtta atctaaagcc aagtttaatc tatttttttt aatcctaatt
     3301 tagctcctct tttcaaaaca aaatcaaaag cagcttgata ttcattcggg atttcgcctt
     3361 ctaaaatagc ttctttcacg gcttctttca aaattccaat ttcacgagaa ggttgcaaat
     3421 tgaaaatagc cataatttct tcgccagaaa tgggcggttg gaaatttcga atgctgtcac
     3481 gttcttccac ttccacgatt ttcttgcgga caatctcgaa attctggtgg tattttttga
     3541 atttgttcgg atttttggta gtgatgtcgg cttcgcacaa agtcatcaag ctttctacat
     3601 cttcgccggc atcaaaaact aggcgacgaa cggccgaatc cgttaccaaa tcttgcgaaa
     3661 gtacaatagg tctcgaactc atcatcacca ttttttgcac aaatttcatt ttatggttca
     3721 acggcattcg taatctttcg aagattttct tggccatttt tccgccaagg aattcgtgcc
     3781 cgtgaaacgt ccaaccttgt tttttgttga agcgtttggt gggtgctttg ccaatgtcgt
     3841 gcaacaatgc tgcccagcgc aaccaaacat cgtccgtatt gggacaaata ttatcaacta
     3901 cttccagcgt atggtaaaag ttgtttttat gcgtttgtcc ttcaatttct tctacttgat
     3961 tcaaagctgt taattcggga agaataatat ctaaaaggcc agttttgtac aacagtaaaa
     4021 atccaacaga aggtttgtcg gtcgaaagaa ttttattcaa ttcatccaca attctttcac
     4081 cagaaataat cttgattcgt tctgcgtttt tggtaatcga attcaaagaa tttgcttcaa
     4141 tttcaaaacc caattgggtc gcaaaacgaa ttcctctgag cattcgcaaa ggatcgtcgg
     4201 agaatgttat gtctggatcg agtggtgttt tgatgttttt gtttttcata tcttccaatc
     4261 cgttgaaagg gtctgacaaa ttaccaaaat tgttttcgtt tagcgataaa gccaatgcat
     4321 tgatggtaaa atcccgacga ttttggtcgt cttccaaagt tccgttttcc acgacaggat
     4381 ttcgactgtc gtgagtgtac gattcttttc tggcaccaac aaattcgatt tcggtgtctt
     4441 caaaacgcaa cattgccgtt ccataagttt taaaaacctg gacttttggt ttgttgggca
     4501 ataattccga aactttcaag gcgagttcaa tgccgctgcc cacagcaacc acatcgatgt
     4561 cttttttgaa atctcgacca agaagtaaat ctctcacgaa accaccaatc acataactat
     4621 caacgttaag ttcttgtgaa gcttgagaaa taacttcgaa tattttatta tttagggctt
     4681 gtttgtaatt catattttat ttgccacgaa tttagcgttt ttgtgaacga aggtttttac
     4741 cgcaaattcg caaattattt tttagaatct atgattcgtt tgtattctaa agatactaaa
     4801 ttgaaattag caagtattgc caatttgttt ttagatactt ttaagtaatt tatactttgg
     4861 ttataatgac tgttgttaaa acaatcaacg gtttttattt cgagaatgat tttatcgtaa
     4921 actacaaagt ctgcgtaaaa tttatgcttt agaatggtgt ctttgtaatt gatagaaaac
     4981 tctttttctc tttggtatgg aatattttgt tcttgaaatt catattcaat agcatcttta
     5041 tatactattt cagaaaatcc tttgccaaga tgtttatgga cttctaatag tattccaaca
     5101 attttataac tttcatcctt atataaatat tcgtcttcca tataaactaa aaataatttg
     5161 cgaatttgcg gttaaaaaaa tgtggctacc ctattaatta gtgtattctc ccgaaaataa
     5221 taaaaaaata atctgcggca aaaaaaacta tttcctaatc acttttacct gcgaatcgct
     5281 tgtcaatttg atgattgttg agggctttcc gttaatttta tcttggtaca aatttacaac
     5341 atagtccacg ccttttataa tttcggggct aatatctttg aaggcaatag gagtgggcag
     5401 ccccgagatg ttggccgagg tggaaaccaa tggttttttc attcgttcca ttagtttaaa
     5461 acagaatggt tctttcacaa ttcggattcc cagcgtttta tcggtggcaa ttaaattggc
     5521 tgctacattt ctaggattgt caagaattag tgtggttggt ttttcggata aatcgataat
     5581 ttgccaagct acttctggaa tgtctttgaa gacgttgtac atcattttct cgccattcat
     5641 caagcaaatc atacttttag aatcatcacg ttgtttgagt ttgtagattt ttgccacggc
     5701 ttcagcattg gtagcatcgc aaccaattcc ccaaacggta tcggttggat agaggataat
     5761 tccgccttgt tgtattattt cgaaagcttt gtgtatttct tcgttaagat tttccatttt
     5821 ttttatttag aattggatag tcgtaatcag gagtgcaaag aaacgaataa taattttgta
     5881 tttgccatat catttgactg taaacagtgt ttttgaactc taaaaaaaca agtaaaatat
     5941 tcttgatttt ttacaatagg aatgacttat ctttgtttct ctaaaattga attagaaatg
     6001 attttaaaaa ttgactcgca ttttgaaaaa tatgcatcaa gtttgttgca aatagttgaa
     6061 aattttaaaa cttcggggct tctttttggc gatggaaagc ggaataccat aaaacttttt
     6121 gagtttgaag acttgacggt taacataaaa tcgtttaaaa ttcctcatct ttttaatcga
     6181 gttgtttatc aatattttcg aaagtcaaag gccagaagat cttatgaata tgccaatgct
     6241 ttgttgaaaa aaggaattgg tacaccacaa cctattgctt attttgaaaa ttcaagttgg
     6301 ttgggtttaa aagatagtta ttatgtaagt gaacatcttg aggtggattt gacatttaga
     6361 gagttagata tacaacctga ttatccagat cacgaaaata tattacgaca gtttactcgt
     6421 ttttctttcg atttacacga aaaaggcata gaatttttag accattcgcc aggaaatact
     6481 ttaataaaaa atggaattaa tggcaattac agtttttatt tagttgattt gaatagaatg
     6541 aattttgata aatctttgga ttttgaaaag agaatgaaaa atttgagtca tttaactgca
     6601 aaaaaagaga tgattagggt tatgagtgat gaatatgcta aactttcagg t
//
LOCUS       DAOSNN010000145         6627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11669_length_6627_cov_5.062538, whole genome shotgun sequence.
ACCESSION   DAOSNN010000145 DAOSNN010000000
VERSION     DAOSNN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11669_length_6627_cov_5.062538"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PLQ70_08095"
     CDS             <1..477
                     /locus_tag="PLQ70_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34613.1"
                     /translation="FIISLAPLFSKWWESKYNPSANNKYGIGMVLLAIGMACIAFGAS
                     GIEPGAKTASVSMIWLILVYLFHTMAELCISPVGLSNVSKLVPARMIAFMFGVWYLAV
                     AIGMKGAGMFGENIDKIADEHGLSYFFWMLTIISVVVAFFAFVMAPVIKKLMHGVK"
     gene            521..985
                     /locus_tag="PLQ70_08100"
     CDS             521..985
                     /locus_tag="PLQ70_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQE34614.1"
                     /translation="MKKIFLTLLLVAGSFAIQAQELKWETDINKAITVSNKTKKPMLL
                     FFTGSDWCGWCIRLQKEVLKTPEFATWAKKNVVLVELDFPRAVPQSDAIKAQNNALQQ
                     TFGIQGFPTIYFATAKVNAKKVPAFTGIGSTGYVAGGPKAWLAVADGILKTK"
     gene            complement(988..3024)
                     /locus_tag="PLQ70_08105"
     CDS             complement(988..3024)
                     /locus_tag="PLQ70_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQE34615.1"
                     /translation="MKVLQFPLARITMGFLAGILVVYYFQPNPTAVFTALFIGFCAFG
                     ILFFISKSKIVNPLFFGFTTYIMAFGIGATTQIIHTNSFQKSNYIYNKAVFEKPHSVS
                     ITIREKLRSSIYNDRYIVIVNRIDNATNTGRLLLNIRKDSLNRPFEIGSHLQITGNLY
                     KSGPAKNPNQFDYGKYLESKKIYAQMYADVSDIKIGSIIKKDIWYYTSKLRSKIIKNL
                     EKNNFNKAELHVAIALILGQQQDISPDIIKDYQYAGAVHILSVSGLHIGFILLFVTFI
                     LKPFPNTKRGSFIKLIIILASLAAFGVIAGLAPSVLRSVTMFSFVAIGMYLKRSTNIF
                     HTLLVSMLLILLFEPSFLFDVGFQLSYIALFFILWLQPILAQLCTPKYKISKYFWEIL
                     TVSFAAQIGTLPLSIYYFHQFPSLFFITNLVIIPFLTVIMALGVLVMVLAALDWLPMF
                     LSKSLEWSIWFMDKIINTIASLEQFIFKEIPFNWQLLLSCYLLIITTIIWFKKPNFNR
                     LVLALIAVIIFQVSYFGNHWMIRNQKELVVFNSKKNTLIAERNGENVILYSNDSLMKT
                     ASKNTTVSTYLMGNFSHLTSTKRVQNLLYFDGNKILIVDSLGVYPKDIHPDIVVMTQS
                     PKINMERFLQTIKPKIVIADASNFRNIQKLWKATCLKAKIPFHATGEKGFYRVE"
     gene            complement(3083..4195)
                     /gene="lpxB"
                     /locus_tag="PLQ70_08110"
     CDS             complement(3083..4195)
                     /gene="lpxB"
                     /locus_tag="PLQ70_08110"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQE34616.1"
                     /translation="MKYYIIAGEASGDLHGANLMKALYKEDPNANIRFWGGDLMQNVG
                     GTLVKHYRDLAFMGFVEVLFHLRTILNNIKICKKDIVDFQPDVLIFIDYPGFNLRIAK
                     WAKPLGFKTHYYISPQIWAWKESRITEIKRDVDKMFVILPFEKDFYEKKHHFLVEFVG
                     HPLIDAIHKHPAIDASVFRKENQLNEKPIIAILPGSRKQEITKMLSVMLSVVNDFPDY
                     QFVIAGAPSQEFSFYKDFITQENIKFVSNKTYDLLSNATAALVTSGTATLETALFKIP
                     EVVCYKGSWISYQIAKRIITLKYISLVNLIMDEEVVTELIQDDCNSKRIREELKKITE
                     PNYRQNLLEKYELLELKLGGIGASEKTAKLIVSDLK"
     gene            complement(4209..4481)
                     /locus_tag="PLQ70_08115"
     CDS             complement(4209..4481)
                     /locus_tag="PLQ70_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34617.1"
                     /translation="MNKTDILIGFAIGILASILGSFLFITFFTDFDFIVGIENMKSEG
                     KLGKLITLGSILDLIAFGVLLKLNKEIMARGVVLAVISIAIFTIFA"
     gene            complement(4474..5253)
                     /gene="surE"
                     /locus_tag="PLQ70_08120"
     CDS             complement(4474..5253)
                     /gene="surE"
                     /locus_tag="PLQ70_08120"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQE34618.1"
                     /translation="MNQDKPLILVTNDDGISAPGIRTLIDVMSKIGTVVVVAPDSPQS
                     ATGHSITINDTLYINKISKENAPILEYSCSGTPVDCVKLAVNEVLKRKPDLCVSGINH
                     GSNSSINVIYSGTMSAAVEAGIEGIPAIGFSLLDYDWNADFEQIKTFIHTISLEVLEK
                     GLPENIVLNVNFPKLKEKEIKGIKICRQAKALWIEKFDKRKTPQGKDYYWLTGEFVNQ
                     DKGEDTDEWALANGYISIVPVQFDLTAHHAIQQLNTWKWNE"
     gene            5471..>6627
                     /locus_tag="PLQ70_08125"
     CDS             5471..>6627
                     /locus_tag="PLQ70_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQE34619.1"
                     /translation="MKRNYKILIAVVLLSATLFAFKINSEKSADPEKDKMLLELLTFV
                     IEKGHYNPAKIDDAFSKGIYKDYIEALDPSKRFFLQSDIDEFSKYETLLDDQLLNKDL
                     TFFNLTYTRLMKRMDEGKELYKGILSTPFDYSIDETFNTDYEKSPYAKNSQELKEKWR
                     KQLKLSTLSSLTDRLKIQENKEKGIVEKVEKSDSDLKSGEMIDNSANTEKDNASDGKP
                     KTFEELEKITRESSLKSLDEYFGFMKDLTRDDWFSVYINSITARFDPHTNYFPAEEKE
                     RFDVSISGKFEGIGARLQKKNDFTEITELISGGPAWRGKLLEAGDVIMKVAQGKSEPV
                     DIVGMRLDDVVKKIKGPKGSEVRLTVKKVDGSIKTISIIRDIVEIEETYAKS"
BASE COUNT         2263 a         1097 c         1175 g         2092 t
ORIGIN      
        1 tttatcattt ctctcgctcc attattttcg aaatggtggg aaagcaaata caatccatct
       61 gccaacaaca aatacggaat cggaatggtt ttacttgcca ttgggatggc ttgtatcgct
      121 tttggagcca gcggaattga acctggagcc aaaacagctt cggtgagcat gatttggtta
      181 atattagtgt atctttttca taccatggcc gaattgtgca tttcgccagt ggggttatcc
      241 aatgtgagca aattagttcc agccagaatg attgcattta tgtttggcgt ttggtatctt
      301 gccgtcgcaa ttggtatgaa aggagcggga atgtttggcg aaaatatcga taaaattgcc
      361 gatgaacacg ggctaagtta ctttttttgg atgctaacaa ttatttcagt tgtggtggca
      421 ttttttgcat ttgtaatggc tccagtcata aaaaaattaa tgcacggagt aaaataacgg
      481 cactattttt gaaactaaaa acgaattaaa ttatataaaa atgaaaaaaa tatttttaac
      541 attactatta gttgcgggct cttttgccat tcaagctcag gaactaaaat gggaaaccga
      601 tatcaacaaa gcaataactg tatctaataa aaccaaaaaa ccaatgttgc tattttttac
      661 cggtagcgat tggtgcggtt ggtgtattcg tttgcaaaaa gaggttttga aaactccaga
      721 gtttgcaact tgggcaaaga aaaatgtggt tttagtagaa ttggattttc ctagagctgt
      781 gccacaatcg gatgcgataa aagcacaaaa taatgctttg cagcaaactt ttggaattca
      841 aggatttcct acgatttatt ttgctactgc aaaagtgaat gcaaaaaaag ttccagcttt
      901 tacaggtata ggaagcacgg gttatgtcgc tggtggtcca aaagcgtggt tggcagtcgc
      961 cgatggcatt ttgaaaacaa aatagtttta ttcaactcta taaaatccct tttcgccagt
     1021 tgcgtgaaaa gggatttttg ctttcaagca agtcgctttc cacagttttt gaatattcct
     1081 aaaattcgaa gcatcggcaa ttactatttt gggttttata gtttgtagaa aacgttccat
     1141 atttattttt ggtgattggg tcattactac aatatcagga tgaatgtctt ttggataaac
     1201 tcctaaacta tccacaatca gaattttatt tccatcaaaa tagagtaggt tttgtactct
     1261 cttcgtactc gttaaatgac taaaatttcc catcaaataa gtggaaactg ttgtgttttt
     1321 tgaggccgtt ttcattagac tgtcattaga atataagatc acattttctc catttcgttc
     1381 tgcaatcagt gtatttttct tcgagttgaa aaccaccaat tctttttggt ttcgaatcat
     1441 ccaatggttt ccaaaatagg aaacttggaa aataattacg gctatcaatg ccaaaaccaa
     1501 tctattgaaa ttcggttttt tgaaccaaat tattgtggta atgatcaaaa gataacaact
     1561 caataacaat tgccaattga atggaatttc tttaaaaata aactgttcta aagatgcgat
     1621 agtattgatg attttatcca taaaccaaat actccattcc aaagatttag acaaaaacat
     1681 cggaagccaa tccaaagctg ccaaaaccat taccaaaacg cccaaagcca ttatcacagt
     1741 caaaaacgga ataatgacca aattagttat aaaaaacaaa cttggaaatt gatgaaaata
     1801 ataaatactt aagggtaaag taccaatttg tgccgcaaac gaaaccgtta gaatttccca
     1861 aaagtatttg ctgattttat attttggagt gcaaagttgg gctaatattg gttgcaacca
     1921 aagtataaaa aacaaggcaa tataactcaa ttggaaaccc acatcaaaca aaaaagaagg
     1981 ctcaaaaagc aaaattagca acatagaaac caacaaagtg tggaaaatat tggtgcttcg
     2041 tttgagatac attccaatag caacaaacga aaacatagtc accgaacgga ggacggatgg
     2101 agccaaacca gcaatcacac caaaagcggc aagcgaagcc agaataataa ttaatttgat
     2161 gaaagaacct cgtttggtgt tgggaaaagg ctttaaaata aaagtcacaa aaagcaaaat
     2221 aaatccaata tgcaagcccg aaaccgacaa aatatgtacc gcaccggcat attgataatc
     2281 cttgataata tcaggcgaaa tgtcctgttg ttgtcctaaa atcaaggcaa tagcaacatg
     2341 gagttcggct ttattgaagt tgtttttctc taggttcttg ataatttttg accgtaattt
     2401 tgaggtataa taccaaatat ctttcttgat tattgaacct attttaatat ctgaaacatc
     2461 ggcatacatt tgagcataga tttttttgct ttcaagatat ttgccataat caaattggtt
     2521 tggatttttg gcaggaccac ttttgtagag atttccagta atttgcaaat gagagccaat
     2581 ttcgaatgga cgatttaagc tgtcttttcg aatatttaac aaaagtctcc cagtatttgt
     2641 tgcattgtca attcgattca caatcacaat atagcggtcg ttgtaaatag aacttcgtag
     2701 tttttctcga atagtaatcg acaccgaatg tggtttttcg aaaacggctt tgttgtaaat
     2761 ataattgctt ttctggaagg aattggtatg gatgatttgg gttgtagctc caattccaaa
     2821 ggccataata taggtcgtga atccaaaaaa tagagggttt acaattttgc ttttagaaat
     2881 gaaaaataaa attccaaaag cgcaaaaacc aataaaaagg gctgtaaaaa cagctgttgg
     2941 attgggttga aaataataaa caacaagaat accggccaaa aaacccattg taattctcgc
     3001 taaaggaaat tgtaatactt tcatattgat aaaagtacgt aatttttaat cagaaataac
     3061 tttgtttaaa ttttaaaaca aattatttta aatcgctcac aattaacttt gcggtttttt
     3121 cgctagcgcc aatgccaccc aattttagtt ctaatagttc atatttttcc aaaagatttt
     3181 ggcgataatt aggttctgta atttttttca attcttcacg aatgcgtttt gagttgcaat
     3241 catcttgaat caattcggta accacttctt cgtccataat caggttgact agcgaaatgt
     3301 attttagagt aatgatgcgt ttggctattt gataggaaat ccagctgcct ttatagcaaa
     3361 ccacttcggg tattttaaaa agagctgttt ccagcgttgc ggttcccgaa gtaaccaatg
     3421 ctgccgtggc attgcttaat aaatcatagg ttttattcga aacaaatttg atattttctt
     3481 gtgtaataaa atctttgtaa aaagaaaact cctgactggg tgcgccagca atcacaaatt
     3541 gataatctgg aaaatcattg accacactca gcatcaccga aagcattttg gtaatctctt
     3601 gtttgcgact tcccggcaaa atggcaataa taggtttttc gtttagttga ttttcttttc
     3661 taaaaacaga ggcatctatc gcaggatgtt tatgaatggc atcaatcaac gggtggccta
     3721 caaattcaac caaaaaatga tgcttctttt catagaaatc tttttcgaaa ggcagaatca
     3781 caaacatttt atcgacatca cgtttgattt cagtaattcg gctttctttc caagcccaaa
     3841 tttgaggcga aatataataa tgcgttttga agccaagagg tttagcccat ttggcaattc
     3901 gcaaattgaa acctggataa tcaataaaaa taagcacatc gggttgaaaa tcgacaatgt
     3961 cttttttgca aattttgatg ttattcaaaa tagttcttaa atgaaaaagc acttcaacaa
     4021 aacccataaa agccaaatcg cgataatgtt tgaccaaagt gccacctaca ttttgcatca
     4081 aatcgccacc ccaaaaccga atattggcgt ttgggtcttc tttgtacaaa gctttcatca
     4141 aattggcacc gtgcaaatct cccgaagctt ctcctgctat tatgtagtat ttcatttttt
     4201 gtagaagttt atgcaaatat cgtaaaaatt gcaatgctaa taactgccaa aaccacgcca
     4261 cgagccatga tttccttatt caattttagt aaaacgccaa aagctatcaa atcaagaatg
     4321 gaacccaatg taattaattt tcccaatttt ccctcagatt tcatgttttc aattcctacg
     4381 atgaaatcaa agtcggtaaa aaatgtgata aaaagaaaac tacctaatat agaagctaaa
     4441 attccaattg caaaacctat taaaatatca gttttattca ttccatttcc aagtattaag
     4501 ttgttgaata gcgtgatgag ctgtcaaatc aaattgaacg ggaactattg aaatgtatcc
     4561 atttgctaga gcccattcat cggtatcttc acctttgtct tgattgacaa attcaccggt
     4621 tagccaataa taatctttgc cttgaggtgt tttgcgcttg tcaaattttt cgatccaaag
     4681 cgctttggcc tgacgacaaa tttttattcc tttaatttct ttttccttta attttggaaa
     4741 attgacgttc aaaacaatat tttcgggtag tcctttttct aaaacttcca aagaaatagt
     4801 gtgtatgaat gtttttattt gctcaaaatc agcattccaa tcgtaatcta ataaagagaa
     4861 acctattgct ggaattcctt caattcctgc ttcgactgcg gcactcattg ttcctgaata
     4921 aataacattt atcgaagaat tcgagccgtg attgatgcca gaaacacata aatctggttt
     4981 tcttttcaga acttcgttaa cggccaattt tacacaatct acgggagttc ccgagcagct
     5041 atattctaaa atgggagcgt tttcttttga tattttgttg atgtacaaag tgtcgttgat
     5101 ggtaatggaa tgtcctgtgg cgctttgtgg actgtcgggc gcaacaacga caacagttcc
     5161 aatcttggac ataacatcaa tcaatgtgcg gattccaggt gccgaaatac catcgtcatt
     5221 agtaactaaa attaaaggtt tgtcttgatt cacaatgaag gtttattatg tattagctct
     5281 aaaatttaaa taaaaagcag ggtgtttttt gtaaacaaag aaacaaaatt aatctagttt
     5341 gaaagttaaa cccaaaaaaa ataaacaatt ttatatcttt aacaaaaatt tatagaaggt
     5401 tttgcttttg ttggcacagt ttttaccgta atttggtttt taaatattta aggatacaat
     5461 tattaacttt atgaaaagaa attataaaat ccttatagcc gtagtcctgc tttcggcgac
     5521 gttatttgca tttaagatta attctgaaaa aagtgcagat ccagaaaaag acaaaatgtt
     5581 gttggaatta ttgacttttg taattgaaaa aggtcattat aacccagcca aaatagatga
     5641 tgctttttca aaaggaattt acaaagatta tattgaagct ttggatcctt ccaaaaggtt
     5701 tttcttgcaa tcggatatcg atgagttttc taaatacgaa acccttttgg atgaccaatt
     5761 gttgaacaaa gatttaacgt tcttcaatct tacctatact cgtttgatga aaagaatgga
     5821 tgaaggcaaa gagttataca aaggtatttt aagcactcct ttcgattatt ctatagacga
     5881 aacttttaat acagattatg aaaaatcacc ttatgccaaa aattcacaag aattgaaaga
     5941 aaaatggcgt aagcaactta agttatctac actttcttca ttgactgatc gtttgaaaat
     6001 tcaggaaaac aaagaaaaag ggattgtaga aaaagtagaa aaaagcgatt ctgatttaaa
     6061 atcaggagaa atgattgata actcagccaa tacagaaaaa gataatgcat ctgatggtaa
     6121 gccaaaaaca ttcgaagaat tagagaaaat cactcgtgaa agttcattga aatctttaga
     6181 tgaatatttt ggttttatga aggatttaac ccgtgacgat tggttttcgg tttatataaa
     6241 ttcgataact gctaggtttg acccacacac aaattatttt ccagctgagg agaaagaaag
     6301 atttgatgtg agcattagtg gaaaattcga aggaattgga gctcgtcttc aaaagaaaaa
     6361 tgattttacc gaaatcactg aacttatctc aggtggacca gcttggagag ggaaactatt
     6421 agaagcaggt gatgttatta tgaaagttgc ccaaggaaaa agcgagccag tagatatcgt
     6481 tggaatgcgt ttggatgatg ttgtcaaaaa aataaaaggc ccaaaaggtt ctgaagttcg
     6541 tttgactgta aagaaagtag atggttcaat aaaaacaata tcaattatta gagatattgt
     6601 agaaattgaa gaaacctatg ccaaatc
//
LOCUS       DAOSNN010000146         6627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11673_length_6627_cov_3.791388, whole genome shotgun sequence.
ACCESSION   DAOSNN010000146 DAOSNN010000000
VERSION     DAOSNN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11673_length_6627_cov_3.791388"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            512..1348
                     /locus_tag="PLQ70_08130"
     CDS             512..1348
                     /locus_tag="PLQ70_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE34620.1"
                     /translation="MITIQNLTFKYKKQDALFTDLSFEQQNGNIVGLLGKNGAGKSTL
                     LQLISGLLKPQKGELSINGYKPFDRNPNFLADIYMVSEEFSFPSINIEVYIKATAPLY
                     PKFDYGKMNNILKEFELDAKKNLNKLSHGQRKKFLIAFALATNCSLLILDEPTNGLDI
                     PSKSLFRKILVSSVSDEQLVLISTHQVKDIETIIDKIVVLDEGKIVYNETVLDISQQW
                     QFKTVASLSGIENLIYQEKCIGGHRIILSSNLEGETEIDIELLFNAIINKVTLKPENY
                     AV"
     gene            1338..2180
                     /locus_tag="PLQ70_08135"
     CDS             1338..2180
                     /locus_tag="PLQ70_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34621.1"
                     /translation="MPFSNYFNIKRFGRLFRQDLLINKTKYGLTILGLGLITYILGYM
                     FLNSSKESMIKHDYAVFQNYTICFAFFMMYVGVIIGSAFPDLSDKIKTSHYLLTPGST
                     FEKVALQFLIRIVLFVPIALGVFWIGICLAKASLIPEIVNYNSVEQLMDPALIPYFDY
                     VFLITQTNGKIWDTWQILFVFFGLFSYGIYLFAGATYFKRYALVKTIIASVVILLTSI
                     TFSVVLSHIFYPKETEGFNTKLNEFVVTGHLTSIELFMVSLSLFSWLFFLAIGYFKLK
                     EKEV"
     gene            2182..2559
                     /locus_tag="PLQ70_08140"
     CDS             2182..2559
                     /locus_tag="PLQ70_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQE34622.1"
                     /translation="MDFKSTKGIYLQIVDNLCQQILEGQLQAGDRVASVRDLAAEFEV
                     NHNTVMRAYTNLQESSIFDNKRGIGFFVSEKAFDLIKANEKANFFSQDLPDFLLKVKL
                     LQLNSTDLAELVSLIKNNDNENQ"
     gene            2546..3241
                     /locus_tag="PLQ70_08145"
     CDS             2546..3241
                     /locus_tag="PLQ70_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34623.1"
                     /translation="MKTSNKIFISFLTFLFSGIIALYIGSKYYVEYYDIHNFVEQEKP
                     LPPFSVVVAEPGAQIQLRNGSKTKIVQGFFKTKQPRFSSFVVRNDTLFVFGVKQDRNK
                     LPRAKFMANVFCENVKHFVAKENSLVFLSGYQVDSLFVKMNDAELVCQNNNSNYIKLI
                     AKNSRINFDCEKLNAMVVELDKSQLELKSKNKINSISGTVKNDSDVKFSMDGKLNLDV
                     DKSSYLQMVDLKN"
     gene            3299..6085
                     /locus_tag="PLQ70_08150"
     CDS             3299..6085
                     /locus_tag="PLQ70_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE34624.1"
                     /translation="MKWLKTYKEFLFPLSTANTNYMKVIFSFFFTLLCFFSSFAQGNI
                     SLTGKILKDKTQIPIESATIYLIRAKDSSVVDYTISDVKGSFKLETKKIPENLILKIA
                     AEGYQEFSQKIEKINANKDFGSLYLTEKINELNEVVIKGMAPPVKVKKDTLEFNAASF
                     KLRPDANVEALLRQLPGVTIDADKKIMVNGKEVNQILVNGKSFFGEDGKVALQNLPAE
                     IIKKVQVSDTKTEEEKFTKQTSTSDKSSINLTIEKDKNKGFFGKLLAGYGTNERYEGS
                     ALMSSFKDKRRISFLSSANNINATGFSMDEVFDNMGGGRNNGSRAPRVGGGSGIIKSN
                     LIGLNYADEWFKGNKSNGNYYFTNMDSENKNKSSALNLLPDTTNRTESNSESNRETTD
                     HNFDFNFEYEINPKTKLLFRPKIVKGLSQSHSEYDSKTWNEDNQLINQSDGTNTDETD
                     KTNFVNDISLFKTMKRKGRYFTANVKTDNTNSDSNSFVNQTTLIILDPDKNVIRDQNV
                     KTVNQKQLYSGNLQYSEPVTDSLTINVQLLSDWEKTTDDKKTFDLDSTTDSYNDLNDL
                     YSSFTTSTKFQIAPKAGFSINKKKFNFRFSTGTYMITLDNHSLYTGTATDLRKNYIIP
                     DATINGSYRFSKTKGLYLFYDRRFSIPKAYQLLPVQDIDNPLNSYTGNPNLEAGKSHY
                     LNLNFSDYDYKSRSGYYIYTGTTYNDNKIINTSIYDKNGRQITTYTNIAGTFTSYLGA
                     NWQKTFKKEAHSFGLDLGLYANYYLDKGYTNIQLFEAKTLGLSPSFRLSYDYGELLTI
                     RPSYTFNYNSTTYDNSSIASSANRFHNAKIETTSKWPKKWIFGNDFGYSYNSNISPGY
                     KKDFYLWNISLSYEFTKDLSLKVKVYDVLDQNQSASRYITPTAVIDSENTVLKRYAMF
                     SLLYKFQNFATKKK"
     gene            6244..>6627
                     /locus_tag="PLQ70_08155"
     CDS             6244..>6627
                     /locus_tag="PLQ70_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34625.1"
                     /translation="MHKIRFVLSFLFWLNFFGNVSFAQTQNPSKKEIRKATKRAVQYL
                     NSEKFEKSLETARWSLHYATVINDDYLIATSYNLIAANFEQLSEVDKAISLYEKGLTF
                     ANNSENDTIKNFINNNLGNVYCFEKK"
BASE COUNT         2307 a         1087 c         1117 g         2116 t
ORIGIN      
        1 aaagaaacaa gaacaagttt tagcgaacaa caaagcatta gaccttggag aagaagtgca
       61 acgaatgagt gaacccaatc ttttgattct tatttcatta cttggaatcg ttctttggtt
      121 tagagaaaga gtgaaattaa gcagactaaa ttaagtgtgt tttttaatag agacgcccca
      181 aggcagatta gtcttggttt tttttttgta tgatttgact ataatttatt ttataactat
      241 ctaattatac aaatcgaaaa cactactcca attgtcacgc tggaagcgtc tcaactttcg
      301 agagcaactt aatagagaca cttccagtgt gacaaataac aagaataagt aaaataagta
      361 ttattgattt ttatcctcta aacccttcta aacccttgca tttcgctaat taaattagtt
      421 ttttcatttt tttttattcc aactgcttgt ttattaaaaa aatatccttt cctttgtgta
      481 ctacaaagct agtacactaa taaaacaggc tatgataaca attcaaaatt taacttttaa
      541 gtacaaaaag caagacgctt tgtttacgga tttgtctttc gagcagcaaa acggaaatat
      601 tgtgggtttg ttgggtaaaa atggagcagg aaaatctact ttgcttcaat tgatttcggg
      661 tttgctaaaa ccacaaaagg gcgaattatc cattaatggt tataaaccct ttgatagaaa
      721 tcccaatttc ttagcagaca tttatatggt ttccgaagag ttttcgtttc cttcgattaa
      781 tattgaagtt tatatcaagg caactgcgcc tttgtatccc aaattcgatt acggaaaaat
      841 gaacaatatt ctaaaggaat ttgaattgga tgccaaaaaa aatctgaaca aactttctca
      901 cgggcaacgt aaaaaatttt tgattgcttt tgcattagct accaattgta gtttgctaat
      961 tctggacgag ccaacaaatg gtttggatat tccgtctaaa agtttattcc gaaaaatttt
     1021 agtcagttcg gtttccgacg agcaattggt tctcatttcg actcatcaag tgaaagacat
     1081 tgaaaccata attgacaaaa tagtagtcct tgatgaagga aaaatagttt ataacgaaac
     1141 agttttggat atcagtcaac aatggcaatt taagactgtt gctagtttat caggcatcga
     1201 aaatcttatt taccaagaaa aatgtatagg tggacatcga ataatattgt cgtcaaatct
     1261 agagggagaa accgaaatag acatcgaatt gctttttaac gccatcatca ataaagtaac
     1321 cctaaaacct gaaaactatg ccgtttagta attatttcaa tatcaaacga tttggtcgtt
     1381 tatttcgcca agatttatta atcaacaaaa caaaatacgg tctaaccatc cttggattag
     1441 gattgataac ctacatattg ggttatatgt ttcttaatag ttcgaaagag agtatgatta
     1501 aacacgatta tgctgtattt caaaattaca ccatttgttt tgcctttttt atgatgtatg
     1561 ttggagttat tattggttca gcttttcctg atttgtcgga caaaattaaa acaagtcatt
     1621 atttactcac tccaggatct actttcgaaa aagtagcact tcagttcctg attcgaattg
     1681 tgttgtttgt tcctattgct ttaggggtat tttggatagg catttgtttg gcaaaagcgt
     1741 ctttaattcc cgaaatagtt aattataatt ctgttgaaca attaatggat ccagcattaa
     1801 ttccttattt tgattatgtt tttctaataa cccaaacgaa tggcaaaatc tgggatactt
     1861 ggcaaatcct attcgtgttt tttggattgt tctcttatgg gatttatctt tttgctggtg
     1921 ctacttattt caaacgctat gcattagtaa aaaccataat tgcttcggtg gttattttat
     1981 tgacttcaat tactttttca gttgtattgt ctcatatttt ttatccaaaa gaaaccgaag
     2041 gttttaatac taaattgaat gagtttgttg tcacaggcca tttaactagt attgaattat
     2101 ttatggtatc actatcacta ttttcttggc tattcttttt ggctataggt tatttcaaat
     2161 taaaagaaaa ggaggtttaa tatggatttt aaatcaacca aagggattta tctgcaaata
     2221 gtggataatc tctgccagca aattttggaa ggacagttgc aagcaggtga cagggtagca
     2281 tcggtgcggg atttggctgc cgaattcgaa gtgaatcaca acacagtgat gcgtgcttac
     2341 accaatttgc aggaatccag tatttttgac aacaagcggg gcattggttt ttttgtatct
     2401 gaaaaagctt ttgacctcat caaagccaat gaaaaagcca attttttcag tcaagattta
     2461 cccgattttc tgctgaaagt aaaattgctc caactcaaca gcaccgattt agccgaatta
     2521 gtatctctaa taaaaaacaa cgacaatgaa aaccagtaac aaaatattca tctctttttt
     2581 gacttttctt tttagtggca ttatcgcttt atatataggt tccaaatact atgttgaata
     2641 ttatgacatc cataattttg tagaacaaga aaaaccgcta cctccgtttt cagtagtagt
     2701 tgcggagcct ggcgcacaaa ttcaattgcg aaatggctcg aaaacaaaaa ttgtgcaagg
     2761 tttttttaaa acaaaacaac ctagattttc ttcctttgtg gttcgaaatg atactctttt
     2821 tgtttttggg gttaaacaag accgtaataa attacctcga gccaaattta tggctaatgt
     2881 tttttgtgaa aatgtaaaac attttgttgc caaagaaaat tcacttgtat ttctgtcggg
     2941 ttatcaggta gattcccttt ttgtcaagat gaatgatgcc gagttggttt gccaaaacaa
     3001 caattcaaat tatatcaaat tgatagctaa aaactctcgt atcaattttg attgcgagaa
     3061 actaaatgcg atggtcgttg aactcgataa aagtcaactg gaattaaaat caaaaaataa
     3121 aataaacagt atctcaggaa cggtaaaaaa tgactcggat gtaaaatttt ctatggatgg
     3181 aaagttgaac cttgatgtgg ataaatcaag ctatcttcaa atggttgatt taaaaaattg
     3241 atttaaaacc taatttttaa cttttaattg taattttttt ctgaatcaaa tattaatttt
     3301 gaagtggtta aaaacttaca aagaattcct atttccttta tcaacagcca atacaaatta
     3361 tatgaaagta attttcagct tcttttttac cttattatgt ttcttttctt cattcgcaca
     3421 gggcaacatt tctttaactg gcaaaatcct aaaagacaaa acccaaattc ccatagaatc
     3481 ggcaactatt tacttgattc gtgccaaaga ttcttctgtg gttgattata cgatttccga
     3541 tgtcaaagga agttttaaat tagaaaccaa gaaaatacct gaaaatctta ttctgaaaat
     3601 tgctgccgaa ggttatcagg aattctccca aaagatcgaa aaaataaatg ccaacaagga
     3661 ttttggttca ctgtatttga ccgaaaaaat aaatgaactc aacgaagtag tgatcaaagg
     3721 aatggctccg cctgtaaaag tcaaaaaaga tacattagag tttaatgcgg cttcgttcaa
     3781 attgcgtccc gatgccaatg tagaagcttt attgcgacaa ttgcctggag taacgattga
     3841 tgcagacaag aaaattatgg tcaatggtaa ggaagtaaac caaatattgg tcaacggaaa
     3901 atcttttttt ggcgaagacg gaaaagtggc actccagaat ttaccagccg aaataatcaa
     3961 aaaagtacaa gtttcagaca ccaaaaccga agaagaaaag tttaccaaac aaacatcgac
     4021 ttcggataaa tcaagtatta atttgacgat tgaaaaagac aaaaacaaag gttttttcgg
     4081 aaaactcttg gcgggttacg gaaccaatga acgctatgaa ggaagtgctt tgatgagttc
     4141 tttcaaggac aaaagacgca ttagcttttt gagttcggcc aacaatatca atgccacagg
     4201 cttttcgatg gacgaggttt tcgacaatat gggtggcgga cgaaataatg gttctagagc
     4261 accaagagta ggaggaggaa gtggcatcat caagtcaaat ttgataggtt tgaattatgc
     4321 cgacgaatgg tttaaaggaa acaaaagcaa cgggaattac tattttacca atatggattc
     4381 tgaaaataaa aacaaatcat cagctttgaa tttgttacca gatacaacca atagaaccga
     4441 atctaattcg gagtcgaata gagaaactac ggatcataac ttcgatttca attttgaata
     4501 cgaaatcaat ccaaagacca aattattgtt cagacctaaa atagtgaaag gtttgagcca
     4561 atcgcattcg gaatacgata gtaaaacttg gaatgaagac aaccaattga tcaatcaatc
     4621 cgatggaaca aatacagatg aaaccgataa aaccaatttt gtaaatgaca tttcactttt
     4681 taaaacaatg aaaagaaaag ggaggtattt tacggctaat gtaaagactg acaataccaa
     4741 ttccgattca aattcgtttg tgaaccaaac aaccttgatt attttagatc ctgataaaaa
     4801 cgtgattcgc gaccaaaatg ttaaaaccgt aaatcaaaag caattgtatt cgggcaactt
     4861 acaatattct gaacctgtaa cggattcgtt gaccataaat gtacaattgc tttcggattg
     4921 ggaaaaaact actgacgaca aaaaaacttt cgacttagat tctactaccg attcttacaa
     4981 tgatttgaat gatttatata gtagttttac cacttctacc aaatttcaaa tagctccaaa
     5041 agcaggtttt agcatcaata aaaagaaatt caattttaga ttttcgaccg gaacatatat
     5101 gattacgtta gacaatcatt cgttatatac aggaacagca accgatttga gaaaaaatta
     5161 catcattcca gacgcaacca taaacggaag ttatcgtttt tcaaaaacca aaggactgta
     5221 tctgttttat gatcgtagat tttcgattcc caaagcctac caacttttgc cagtgcaaga
     5281 catagacaat ccgctgaata gttatacagg aaaccctaat ttagaagcag gtaaatcgca
     5341 ttatttgaac ctaaattttt ctgattatga ctataagtcc cgttcaggtt attatatata
     5401 cacaggcaca acttacaatg acaataaaat tattaacact tctatctatg acaaaaatgg
     5461 aagacaaata accacttaca ccaatatcgc aggaacattc accagttatt taggggcaaa
     5521 ttggcagaaa acctttaaga aagaagcgca ttcatttgga ctagatttag gcttgtatgc
     5581 gaattactac ttagacaaag gatataccaa cattcaattg tttgaagcca aaacattagg
     5641 attgtcgccc agtttcagat tgtcttacga ttatggcgaa ttgttgacta ttagaccttc
     5701 ttatactttt aattacaaca gcaccactta tgataatagc tcgatagcct caagtgcgaa
     5761 tagatttcac aatgccaaaa ttgaaaccac cagcaaatgg ccaaaaaaat ggatttttgg
     5821 aaatgatttt ggctactctt ataattcgaa tatttcgcca ggctacaaaa aagattttta
     5881 cctctggaac atcagtttgt cctatgaatt tacaaaagac ttgagtttaa aagtgaaagt
     5941 gtatgatgta ctcgaccaaa accaaagcgc ttcaagatac atcacgccaa cggcagttat
     6001 cgattcagag aatacggttc taaagcgtta tgcgatgttt tctttgttgt ataaatttca
     6061 aaattttgca accaagaaga aatagattgt tttatgagga gcaatctttt ttgttctacg
     6121 ttctacgttc aaccaatgaa aggtattttt aatcattata attagggaat aacctataaa
     6181 tatctctatt taaatagttc taatacattt ttatgctgta tatttgaaat gattttgata
     6241 catatgcata aaattcgttt cgttttatcg tttctttttt ggctgaattt tttcggcaat
     6301 gtatcatttg cacaaaccca aaacccttct aaaaaagaaa tacggaaagc tacaaaacgg
     6361 gcagttcaat acctcaattc agagaaattc gaaaaatcgt tagaaaccgc aagatggagt
     6421 ttgcattatg caacagtcat taatgatgat tatcttattg caacttctta taatttgatt
     6481 gcagccaatt tcgaacaatt atcggaggtt gacaaagcaa tttcattgta tgaaaaaggg
     6541 ttgacttttg ctaataattc cgaaaacgat accattaaaa atttcatcaa taataatttg
     6601 gggaacgtct attgttttga aaaaaaa
//
LOCUS       DAOSNN010000147         6612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11703_length_6612_cov_4.880281, whole genome shotgun sequence.
ACCESSION   DAOSNN010000147 DAOSNN010000000
VERSION     DAOSNN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11703_length_6612_cov_4.880281"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /gene="ribB"
                     /locus_tag="PLQ70_08160"
     CDS             complement(<1..1129)
                     /gene="ribB"
                     /locus_tag="PLQ70_08160"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HQE34626.1"
                     /translation="MKTSKIKLNTIEEAIEDIRQGKIIIVVDDESRENEGDFLAAAEK
                     VTPEMINFMATHGRGLICAPITENRCKELGLSSMVHNNTDPMETAFTVSVDLRGNGVT
                     TGISAADRAKTVLSLVAPNTKPHELARPGHIFPLIAKQGGVLRRTGHTEAAIDFARLA
                     GFKPAGLIVEIMNEDGSMARLPELVKVAKKFNLKLVSIEDLVAYRMQHDSLIVKKEDF
                     DIETRFGTFRLRAYLQTTNNRVHIALTKGTWNIGEPVLTRINATLINNDILGTLTNDA
                     NKKLCDLFELINADGKGAVLFINQESQSGDLLNRIVELKELQSKGIFKAPQIKMDNRD
                     FGIGAQILHDIDISKIRLLSNTEQTKRVGMIGYGLEIKEYVK"
     gene            complement(1137..2573)
                     /locus_tag="PLQ70_08165"
     CDS             complement(1137..2573)
                     /locus_tag="PLQ70_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQE34627.1"
                     /translation="MKILDKYLLKTFLSTFLMVFIILLFIFILQSVWLFIAELAGKDL
                     DFPMVIKFLLFAIPRIIPLVLPLSVLLSSIMTFGNLAENYEFAAMKSAGISLQRAMRS
                     LIIFITILSIVAFTFANNVIPYAEYKFINFRRNIAQLKPAMAIAEGQFSDVGLYSIKV
                     NKKSGVNGNTLTGITIHKKSPMGEGNKTVIKAKNGLLISSEQSNTLQMVLNDGYYYED
                     VIPKKYEDRIKVPFAKSSFKKYNINIDLSKLNKVDVNNESVSNTNTMLNISELNYTLD
                     SLHKNMKTEVLSFSENINQRIGMQRIIKNDLVFNKTKTLPSNLLSKYSNDKKLNILNF
                     AISNIENNLLSISVTNSELEFKQKNINGHILAFYDKFVIAFACFLMFFIGAPLGAIIR
                     KGGLGLPIVFAVLIFITFHFINTFGKRISQENQLSPFLGAWMSTLILLPLAILLTYRA
                     TNDIGLINMDIIMAPFQKIYQKIFPPSK"
     gene            complement(2580..3272)
                     /locus_tag="PLQ70_08170"
     CDS             complement(2580..3272)
                     /locus_tag="PLQ70_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HQE34628.1"
                     /translation="MKSKIHEYRNDKKKSFSKSFFLITTALLLFSFSIQAQENKAKDL
                     LNEVSAIVKGYKNIVIDFKYSLNNTKENIKQDSKGNVTMMGNQYVLNFMGATKIYDGK
                     KSYTIVPEDEEVTISTVNEKDDSAITPSKMLTFFNSGYRCTIDKLEDVKGRKIQYVKL
                     TPNNTKDKRKEILLGIDVQTKHIYNLIETDKKGTKTTLTVNSFKTNQPLSKNQFTFVA
                     SKYPNYYINKID"
     gene            complement(3282..5738)
                     /locus_tag="PLQ70_08175"
     CDS             complement(3282..5738)
                     /locus_tag="PLQ70_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HQE34629.1"
                     /translation="MAKTIKSVTSDKKSDTNSVAKKSWAITKQHKIVLGSLLVLFSIA
                     LLLAFLSFYIHGQADQSAVNQLSDRTETVQNWLGKFGAFLADFFVYKGFGLASFLFVR
                     LFFLTGVFLVLDISLKKLKKIWFWDLFVIIIVSVLFGFFATSLPELGGTIGYELNLFS
                     QDYLGKTGTLLVLIFGLIVYLIFKIKLSPERIKSFFENTKKEIKNDLNSASTTNPATS
                     AYNLEEYAVEENQEDEDIKGIQLKTIPVSQFEINKEALKPTISNASEITLKPIPKEVT
                     PPPFNIPEIIQTEDEHFVIEKAPEEDIIEENLASKLVADFGLFDPTLDLSNYKYPTID
                     LLKEYSTGGITINQEELEENKNRIVETLRNYKIEIAQIKATVGPSVTLYEIVPEAGIR
                     ISKIKSLEDDIALSLSALGIRIIAPIPGKGTIGIEVPNKNPTMVSMKSVIGSAKFQEA
                     EMELPLALGKTISNETFVVDLAKMPHLLMAGATGQGKSVGLNAVITSLLYKKHPAEIK
                     FVLVDPKKVELTLFNKIERHYLAKLPDTDDAIITDNAKVVNTLNSLCVEMDNRYSLLK
                     DAMVRNIKEYNEKFKSRKLNPENGHRFLPYIVLVVDEFADLIMTAGKEVEVPIARLAQ
                     LARAIGIHLIIATQRPSVNVITGLIKANFPARIAFRVSSKIDSRTILDTQGADQLIGR
                     GDMLFHGGNDVVRVQCAFVDTPEVEKIVDFIGSQKAYATAYLLPEFVGEETGINLDMD
                     ISERDTLFREAAEIIVMAQQGSASLLQRKLKLGYNRAGRLIDQLEAAGIVGPFEGSKA
                     RSVNILDMNGLEQFFNNEQN"
     gene            complement(5757..6131)
                     /locus_tag="PLQ70_08180"
     CDS             complement(5757..6131)
                     /locus_tag="PLQ70_08180"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQE34630.1"
                     /translation="MEFQKDTSFFSGRLKSVSFAVKGAVKLITTEHSVMTQFFIGIMM
                     TILGFCMHISATEWLFQILAIGLVMSIEGINTAVEKIADFIHPNYHERIGFIKDIAAG
                     AVFFAALTAIAIGCIIYVPKFL"
     gene            complement(6139..>6612)
                     /gene="tpx"
                     /locus_tag="PLQ70_08185"
     CDS             complement(6139..>6612)
                     /gene="tpx"
                     /locus_tag="PLQ70_08185"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HQE34631.1"
                     /translation="NPIHTSGELPKVGTKLADFKLVKNDLTIATLSDFAGTNLVLNIF
                     PSIDTGTCATSVRTFNVSAAKLENTKVLCISRDLPFAQKRFCGAEGIENVVNLSDFND
                     GSFGKTNGLEIADGVLAGLHSRCIIVVDASGSVLYTEQVPEIANEPNYEAALAVL"
BASE COUNT         2078 a         1168 c         1042 g         2324 t
ORIGIN      
        1 acttcacata ctctttgatt tcaagaccat atcctatcat tcccacacgt ttggtttgct
       61 cagtatttga tagtaatcta atttttgaaa tatctatatc gtgcaaaatt tgggcaccaa
      121 ttccaaaatc acgattgtcc atcttgattt gtggggcttt gaaaattcct ttggattgca
      181 actctttcaa ttcaacaatt cgatttagta aatcacccga ttggctttcc tgattgatga
      241 acaatacagc tcctttgcca tcggcattga ttaattcaaa aaggtcgcat aattttttat
      301 tggcatcatt agttaacgtt cccaaaatat cattattgat taaagtcgca ttaatacgag
      361 tcaaaacggg ttcaccaata ttccaagtgc cttttgtcag ggctatatgg acacgattat
      421 ttgtggtttg taagtaagct ctaagtctga aggtaccaaa acgtgtttct atatcaaaat
      481 cctctttttt gacgatcaaa ctgtcgtgct gcattcggta agcaaccaaa tcttcaatag
      541 aaactaattt taaattaaat ttcttggcca ctttgactaa ttccggtaaa cgtgccatcg
      601 agccgtcttc attcataatt tcgacaatca gtcccgcagg tttaaatccg gctaaacgag
      661 caaaatctat agcagcttct gtgtgtcctg ttcgtctcaa tacacctccc tgtttggcta
      721 ttaaaggaaa tatatgtcct ggtctggcaa gttcgtgggg tttggtgtta ggggcaacta
      781 aggacaaaac tgttttggct ctatcagcag ccgaaatacc tgtggtaaca ccgtttcctc
      841 tcaaatcaac cgatacagtg aaagcggttt ccataggatc ggtattgtta tgaaccattg
      901 atgacaatcc caactcttta catctatttt cggttattgg ggcacaaata agtcctcggc
      961 catgggtagc cataaaattg atcatttctg gagttacctt ttcggcagct gcaagaaagt
     1021 ctccttcatt ttcacgactc tcatcgtcaa caacaataat aattttgccc tgacgaatat
     1081 cttcaatggc ttcttctatg gtgttgagtt ttattttgga ggttttcata tagtaattat
     1141 tttgatggag gaaatatttt ttgatatatt ttttgaaaag gagccataat tatgtccata
     1201 ttaattaatc caatatcatt ggtagcccga taggtcagta gtatggctaa tggtaaaagt
     1261 attaatgtgg acatccaagc tcctagaaat ggagaaagct gattttcttg tgaaattcgt
     1321 ttgccaaaag tattaataaa atggaaagta ataaagatta atacagcaaa tacaataggc
     1381 aatcctaatc ctccttttcg aatgatagca cccagtggag cgcctatgaa aaacattagg
     1441 aaacaagcaa aagcaattac gaatttgtca tagaaagcta aaatgtgacc attaatgttc
     1501 ttttgtttga attctaattc ggaattagta accgaaattg aaagcaaatt gttttcaata
     1561 ttgctaatgg caaagtttag tatatttaac tttttatcat tcgaatactt agataataaa
     1621 ttactaggga gcgtcttggt tttattgaaa actaaatcat ttttgataat tctttgcata
     1681 ccaatacgct gattgatgtt ttctgaaaag gaaagcactt ctgttttcat gtttttatgt
     1741 aaggaatcta aagtataatt tagttcactt atattaagca tagtatttgt atttgatacg
     1801 ctttcattat taacatcaac cttatttaat tttgacaaat caatattgat attgtatttt
     1861 ttgaaggagc ttttagcaaa tggaactttg atgcgatctt catacttttt tggaatgaca
     1921 tcttcatagt agtaaccatc attcaaaacc atttgcaatg tatttgattg ttcgctactt
     1981 attaataaac catttttagc ttttataaca gttttattac cttcacccat aggtgatttt
     2041 ttatgtattg taattccagt tagggtgttc ccattaacgc cagatttctt gtttaccttt
     2101 atagaataca aaccaacatc gctaaactga ccttcagcaa tagccattgc gggtttcaat
     2161 tgagcaatat ttcttctaaa attaatgaat ttatattcgg cataggggat gacgttattg
     2221 gcaaaagtaa aggctacaat acttaaaatt gtaatgaaaa taattaaact cctcatcgct
     2281 cgttgcaacg aaattccagc cgatttcata gcagcaaatt catagttttc tgccaagttt
     2341 ccaaaagtca ttattgagga caataatact gatagtggca atactaatgg aataattcgg
     2401 ggaatggcaa ataacaaaaa cttgattacc attggaaaat ctaaatcttt accagctaat
     2461 tcggcaataa atagccatac cgattgcagt ataaatataa aaagcaagat aataaatacc
     2521 attaaaaagg tactcagaaa agtctttagt aagtatttgt cgagaatttt cacttagaat
     2581 taatctattt tattgatgta gtaattggga tatttactcg caacaaatgt aaattgattt
     2641 ttcgacaatg gttggttggt tttaaaagaa ttaacagtca atgtagtttt tgtccctttt
     2701 ttgtctgttt caatcaaatt atagatgtgt ttggtttgca catcaatgcc caatagaatt
     2761 tcttttcttt tgtccttcgt gttgtttggg gtcagtttta catactgaat ttttctccct
     2821 ttgacatctt ctaatttatc tattgtacat ctatatcctg aattaaaaaa ggttagcatt
     2881 tttgatggag tgatagcgct atcgtctttt tcatttactg ttgaaatagt aacttcctcg
     2941 tcttctggaa ctattgtata ggattttttt ccgtcataga ttttagtagc ccccataaaa
     3001 ttcaaaacat attggttgcc catcatcgtt acatttcctt tgctatcttg ctttatgttt
     3061 tctttggtat tgttaagact gtatttaaaa tcaattacaa tattcttgta tccttttaca
     3121 attgcggata cttcgttcaa aaggtctttc gctttatttt cttgagcttg aattgaaaaa
     3181 ctgaataaaa gcaatgcggt agtaattaga aaaaaggatt tactaaagct tttcttttta
     3241 tcgtttctgt attcgtgaat cttcgatttc atttttaatt tttaattttg ttcattattg
     3301 aaaaattgtt caagaccatt catatcaaga atgttaacac ttctagcttt gctaccttca
     3361 aatgggccta caattccggc agcttctaat tgatctatca atcgtccggc tctgttataa
     3421 cctaatttta gtttcctttg aagtaatgag gctgatcctt gttgcgccat cacaattatt
     3481 tctgcagctt ctctaaataa tgtatctctt tcggaaatat ccatatcaag attaatgcca
     3541 gtttcttctc caacaaattc tggaagtaaa taagcggttg cataagcttt ttgtgaacca
     3601 ataaagtcaa caattttttc tacttctgga gtatctacaa atgcacattg tacacgaact
     3661 acatcattac cgccgtggaa taacatatca ccacgtccta tcaactgatc tgctccttgg
     3721 gtgtctaaga tggttctgga atcaatttta gaggatactc taaaggcaat tctagctggg
     3781 aaattggctt tgattaatcc cgtaattaca tttaccgaag gtctttgagt agcaataatc
     3841 aaatggattc caatggctct ggctaattgt gctaatcttg ctattggaac ttcaacttct
     3901 tttccagctg tcataattaa atcagcaaac tcatcgacta ccaatacaat gtagggtaaa
     3961 aagcgatgtc cgttttcagg atttaatttt ctagatttaa atttctcgtt gtattcctta
     4021 atatttcgaa ccatcgcatc ttttagcaaa gaatagcgat tatccatttc cacacaaagt
     4081 gaattcaaag tattgacaac tttggcatta tctgtaataa tagcatcatc agtatcgggt
     4141 agtttggcca aataatgtct ttcaatttta ttaaaaagtg tcaattctac tttcttagga
     4201 tcgaccaaaa caaatttgat ttcagcagga tgttttttgt ataataatga ggtgattaca
     4261 gcattcaaac ctactgattt tccttgtcct gttgctccag ccatcaataa gtggggcatc
     4321 ttggctaaat ctactacaaa agtttcattc gaaatggttt ttcctaaagc aagaggcaat
     4381 tccatttccg cttcctgaaa tttggctgaa ccaatcacac ttttcatcga aaccattgta
     4441 ggattcttgt ttggaacctc aataccaatc gttccttttc caggaatagg agcaatgatt
     4501 cgaatcccta atgccgatag tgacaaggca atatcgtctt ctaaactctt aattttcgaa
     4561 attctaattc cagcctcagg aactatttca tatagtgtaa ccgaaggtcc aactgtggct
     4621 ttaatttgtg caatttctat tttataattg cgtaatgttt caacaattct atttttattt
     4681 tcttctaatt cttcttgatt gatggtaatt cctcctgttg aatattcttt taataaatca
     4741 attgttggat acttgtagtt ggataaatcc aaagtggggt cgaataatcc aaaatcggcc
     4801 accaattttg aagctaaatt ttcttcgata atatcttctt ctggcgcttt ttcgattaca
     4861 aaatgctcgt cttctgtttg gattatttcg ggaatgttaa atggaggtgg tgttacttct
     4921 tttggaattg gttttaaagt aatttcagat gcattactaa tggttggttt taaggcttct
     4981 ttgttaattt cgaattgaga aactggaatg gtttttaatt gaattccttt tatatcttcg
     5041 tcctcctgat tttcttcaac ggcatattct tctagattat aagcacttgt agcaggatta
     5101 gttgtcgaag ccgaatttaa atcgttttta atttcttttt ttgtgttttc gaaaaaagat
     5161 tttatcctct caggagacag tttaatttta aaaatcaaat agacaattaa tccaaagatt
     5221 aaaaccagta aggttcctgt tttaccaaga taatcctgcg aaaacaaatt caattcataa
     5281 ccaatagttc cacccaattc aggtagggaa gtggcaaaaa atccgaacaa taccgatact
     5341 ataataatta cgaataaatc ccaaaaccaa atttttttaa gcttttttaa agaaatatca
     5401 agaactaaaa agacacccgt tagaaaaaac aatcgaacaa atagaaagga agccaatcca
     5461 aaacctttgt aaacaaaaaa atcggctaaa aaagcaccaa attttcccaa ccaattctga
     5521 accgtttctg ttctatcaga taattgatta acggcacttt gatcggcttg accatggata
     5581 taaaaggata aaaatgcaag caataaagca attgaaaata acaccaaaag cgatcccaaa
     5641 acaattttgt gctgttttgt aatcgcccat gatttttttg ctacagaatt tgtgtcagat
     5701 tttttatctg aagtaactga ttttattgtt tttgccattc taggtcgaac tgttttctat
     5761 aaaaattttg gtacataaat gatacatcca attgcaattg cagttaaagc agcaaaaaat
     5821 accgcaccag ctgcaatgtc tttaataaat ccaatgcgct cgtgataatt aggatgaata
     5881 aaatcggcaa tcttttcgac agcagtatta attccttcta tactcattac taatcctatg
     5941 gctaatattt gaaataacca ttcagtagca cttatatgca tacaaaaacc aagaattgtc
     6001 atcattatac caataaaaaa ctgagtcatt acactatgtt cggttgtaat taattttaca
     6061 gcacctttca cagcaaatga aacgcttttt aatcttccgc taaaaaaaga agtgtctttc
     6121 tgaaattcca tccgttaatt aaagtactgc taaagcagct tcataattag gctcgttggc
     6181 aatttcagga acttgttcag tatagagtac agaaccactc gcatcaacaa caataataca
     6241 tctggagtgt aaacctgcta aaactccgtc ggcaatttct aaaccattag ttttaccaaa
     6301 actaccatca ttgaaatcag ataaattaac cacattttcg attccttctg ctccacaaaa
     6361 acgtttttgg gcaaatggta aatctctgga gatacacaat actttggtat tttctaattt
     6421 tgcagcagaa acattaaacg ttcttacaga agttgcgcaa gttccagtat caatacttgg
     6481 aaaaatattt agcactaaat ttgttcctgc aaaatcactc aatgtagcaa tagttaaatc
     6541 gtttttaact aatttaaaat cagctagttt tgtgccaact tttggtaatt cgcctgaagt
     6601 gtggattgga tt
//
LOCUS       DAOSNN010000148         6546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11864_length_6546_cov_4.695578, whole genome shotgun sequence.
ACCESSION   DAOSNN010000148 DAOSNN010000000
VERSION     DAOSNN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11864_length_6546_cov_4.695578"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..467
                     /gene="umuD"
                     /locus_tag="PLQ70_08190"
     CDS             27..467
                     /gene="umuD"
                     /locus_tag="PLQ70_08190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HQE34632.1"
                     /translation="MSLKKDQKLTFFTPDFESELRIPFIKEGVSAGFPSPAADFMENG
                     IDLNKELSENPLATFYIKVKGNSMIDAGINDKDVLVVDRSLEPQNNKIAICFIDGEFT
                     VKRIQVGKDCLYLMPENPNYAPIKVTEENQLIIWGIVTYVIKKV"
     gene            complement(503..582)
                     /locus_tag="PLQ70_08195"
     tRNA            complement(503..582)
                     /locus_tag="PLQ70_08195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(546..548),aa:Leu,seq:caa)
     gene            874..1815
                     /locus_tag="PLQ70_08200"
     CDS             874..1815
                     /locus_tag="PLQ70_08200"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQE34633.1"
                     /translation="MSHTDPEAKIFACSQSEYLAEKIAKEYGIPLGKMTMSTYSDGEF
                     QPSYEESIRGLRVFIVCSTFPTADNLMELLLMIDAAKRASARHITAVMPYFGWARQDR
                     KDKPRVPIGAKLVAKLLETAGATRIMTMDLHADQIQGFFEKPVDHLFASTIFLPYVES
                     LGLENLTIASPDMGGSKRAYAYSKFLESDVVICYKQRKVANVIDTMELIGEVKGKNVI
                     LVDDMIDTGGTLAKAADLMIEKGALSVRAICTHPILSGDAYEKIENSKLVELIVTDSI
                     PLKKESKKIKVVSCATLFAEVMYMVHHNNSISGKFLM"
     gene            1885..2493
                     /locus_tag="PLQ70_08205"
     CDS             1885..2493
                     /locus_tag="PLQ70_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HQE34634.1"
                     /translation="MKSITIKGSERESVGKVATKALRNAGAVPCVLYGGNQAVHFSAD
                     ERAFKTLVYTPNAHTVVIELGDKSFNAILQDIQVHPVSDKILHIDFFQIQDDKEITME
                     VPVKIVGNSKGVMAGGDLRMNNRKLKVKALPKNLPDYVEADITPLEMGNKLYVTKVPT
                     PNFKIMHPDNTVICQVKISRAAMKAAQEAAKAAKAPAKGKKK"
     gene            complement(2611..3741)
                     /locus_tag="PLQ70_08210"
     CDS             complement(2611..3741)
                     /locus_tag="PLQ70_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQE34635.1"
                     /translation="MEIDRLALDLILQKLQPNKVIVLLGARRVGKTELIKKLVEQLNE
                     KVLFLNGDDIESHSLLEIQSTANFKRLLGDTKFLIIDEAQEIPAIGKKLKLMVDTIQD
                     LKILVTGSSAFEINNQLGEPLVGRMKTINLYPIAQMEYAKNENLLQTRSNLEDRLILG
                     SYPELSHITNREEKISYLKELVNTQLLRDIFAFEGIKKRDKIIALLQIIAFRTGSELS
                     LESVGRDLQISKNTVEKYLDLFSKVFIIYSVSGFSRNRDNEITKMKKWYFVDNGIRNA
                     IINTFNPLNSREDVGKLWENYLNSERMKKMSYTQNFVYDYFWRTHTKQEIDRIEEKNE
                     QLAAFEYKYGKAKAKIPTEFAKSYPEATFEIINQDNYLDYIL"
     gene            3912..4538
                     /gene="pth"
                     /locus_tag="PLQ70_08215"
     CDS             3912..4538
                     /gene="pth"
                     /locus_tag="PLQ70_08215"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQE34636.1"
                     /translation="MINWFNNLFSRTEITDNKEYMKKFLIVGLGNIGAEYVNTRHNIG
                     FKVVDFLAKKEGISFETVKLGALAEYKFKGRTFLLLKPNTYMNLSGKAVQYWMDKENI
                     PLENIMVITDDLNLSFGTIRIRKKGSDGGHNGLKNINLVLNTQQYTRFRFGISDEFKK
                     GKQVDYVLGEWDDTEKAALPERLELASEIIKSFGTAGIENTMTTFNGK"
     gene            4657..>6546
                     /locus_tag="PLQ70_08220"
     CDS             4657..>6546
                     /locus_tag="PLQ70_08220"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HQE34637.1"
                     /translation="MKTTTPLSIKFIFIFFCMLTLGNIYSQDKNTTTKKIFGQTLKSS
                     KNPNSDKIRCVSTEYEQFLQEKNPKRMSNAQFETWLAPLVSKYKATKANSKTAATIIT
                     IPVVVHVVHNGQTVGIAPNITDAQVQSQITVLNQDFRRMLGTPGYNTNPVGADVEIEF
                     VLAQQDPSGNPTNGIERVSFLQSSWSDTDIENILKPATIWDPTMYLNMWSLKFTDNTV
                     LGYAQFPDGSGLAGLNSSGGSANTDGVVSSYDVFGSKDYGSSFLLVTSLYNKGRTMSH
                     EVGHWLGLLHIWGDGGSRDNNTKDCNASDYCADTPQAGWENYDCNTYDSCPANLGNDM
                     VENYMDYTNDDCMNIFTQNQKDRIAVIMNNAARRSTLKTSTKDDAMSLFATDAEVKIL
                     SFNSNIDYGCATPPAPTNKQVSIYNRGTSTLTSVTLNYNINGGSNQTYTWSGSLTQNQ
                     FADITLPNTNLNGTLNVSIATSNGGTDQRTTNNTASKNYFNNVPTNYNYTNYVFRLQQ
                     DLWGSETTWSLKNSSGTTLYSGGPYEDKPSLPLPSLITENWTLPSNQCYTFTINDSQS
                     DGICCGTDGNGYYDIKTNNGAITVKSGSSFSSSESITITNNTLGTDTFEALNEIYLYP
                     NPTKGI"
BASE COUNT         2271 a         1187 c         1136 g         1952 t
ORIGIN      
        1 aatttcaacg atattctcga aatacgatgt ccctaaaaaa agaccaaaag ctaacgttct
       61 tcactccaga ttttgaaagc gaactgcgaa ttcctttcat aaaagaaggt gtttcggcag
      121 gattcccctc ccctgcggct gattttatgg aaaacggcat cgatttgaac aaagaattga
      181 gcgaaaatcc tttagctact ttttacatca aagtcaaagg caactcgatg attgacgccg
      241 gcatcaacga caaagatgtt ttggttgttg accgaagtct cgaaccccaa aacaacaaaa
      301 tcgccatttg cttcatcgac ggtgaattta ccgtgaaacg catccaagtc ggaaaagact
      361 gtttatatct aatgccggaa aacccaaatt acgctcccat aaaagtcaca gaagaaaacc
      421 aactaattat ttggggcatt gtgacttatg tgattaaaaa agtataaaac caaaaaaacc
      481 gcttctttcg aagcggtttt tgtacccgga gccggagtcg aaccggcacg gtttcccaca
      541 ggtgtttgag accagcgcgt ctaccaattc cgccatccgg gcttcagcag acagaaataa
      601 caacagttat ttcaacgcat taaaaagaga taactgttat aaaaacaact cgcttttgtt
      661 taacacggtg caaatgtaaa aaataaaatt aaagattcta acaaaatact cgaatttttc
      721 ctttcagagt tcgattttat tcctaaattt gcacctcggt aaaacgaaaa acaactaact
      781 aactacatcc gaggcattta tatttttctg gatttaatca aatgataaag ccaaattaaa
      841 tagaatgtaa cggaataaaa aaacaacaaa caaatgtcac atacagatcc agaagctaaa
      901 atttttgcgt gttcacaaag tgaatacctt gcagagaaga tagccaagga atacggaatt
      961 cctttgggta aaatgacaat gtcaacctat agcgatggtg aatttcaacc atcttatgaa
     1021 gaatccataa gaggtttgcg agtatttatt gtttgttcaa catttccaac tgctgataat
     1081 ttgatggaat tgttactaat gattgatgct gccaaacgag cttcggctag acacattaca
     1141 gcagttatgc cttattttgg ctgggcaaga caagatagaa aagacaaacc aagggttccg
     1201 attggagcaa aattagtggc taaattatta gaaactgcgg gagcaacaag aattatgacc
     1261 atggatttgc atgcagacca aattcaagga ttctttgaga aaccagtaga tcaccttttt
     1321 gcttcaacta tatttttacc ctatgtagaa agtctaggtt tagaaaacct aactatagct
     1381 tcaccagata tgggtggttc caaaagagct tatgcttatt ctaagttttt agagtcggat
     1441 gtggtgattt gctacaaaca acgaaaagta gccaatgtga ttgacactat ggagctgatt
     1501 ggtgaagtaa aaggcaaaaa tgtaattttg gtagatgaca tgatcgatac tggaggcaca
     1561 ttggcgaaag ccgcagattt aatgatcgaa aaaggagcat taagcgttag agcaatttgt
     1621 actcacccta ttttgtcggg agatgcatac gaaaaaatag aaaattcgaa attagtcgaa
     1681 ttaattgtta ctgattctat tccgttaaag aaagaatcca aaaaaataaa agtggtgagt
     1741 tgtgccacac tttttgcaga agtaatgtac atggtacacc acaacaattc tattagtgga
     1801 aagtttttga tgtaattcat ttacacaaaa gccaaaaact aattcaaaaa cgagtttaat
     1861 aattaactat ataaattttt tacaatgaaa tcgattacaa ttaaaggatc agaaagagaa
     1921 agcgtgggca aagttgctac taaagcctta cgtaatgctg gagcggttcc ttgcgtgtta
     1981 tacggaggaa atcaagcagt tcatttctca gcagacgaaa gagcattcaa aactttggtt
     2041 tacactccaa acgcacacac agttgtgatt gaacttgggg acaaatcatt caatgctatt
     2101 cttcaagaca ttcaggtaca cccagtatct gacaaaatct tacacattga cttctttcaa
     2161 attcaagatg acaaagaaat cactatggaa gttcctgtaa aaatcgttgg taactcaaaa
     2221 ggagttatgg ctggtgggga tttgcgtatg aacaaccgta aattaaaggt aaaagcttta
     2281 ccaaaaaacc ttcctgatta cgttgaggca gacattacac ctcttgaaat gggtaacaaa
     2341 ttatatgtta ctaaagtgcc aacgccaaat ttcaaaatca tgcacccaga caatactgtg
     2401 atatgtcaag tgaagatttc tcgtgcggct atgaaagcag ctcaagaagc agcaaaagca
     2461 gcaaaagctc ctgcaaaagg aaagaaaaaa taatactttt ttcaataatt caagaaagca
     2521 tcaatctttc gattgatgct tttttgtttt agagaatttc tacaaaagcc accttcaact
     2581 gaattatctt tcgaaaaaaa ccaccacaac ttacaatata taatccaaat aattatcttg
     2641 attgatgatt tcgaaagttg cttctggata ggattttgca aattcggttg ggatttttgc
     2701 tttagctttt ccgtatttgt attcaaaagc ggctaattgt tcattttttt cttctattcg
     2761 gtctatttct tgtttggtat gggtacgcca aaagtaatcg taaacaaaat tttgggtata
     2821 actcattttt ttcattcttt cggaattcaa atagttttcc caaagtttac ccacatcttc
     2881 tctactattc aatggattaa acgtattgat aatggcgttt cggataccgt tatctacaaa
     2941 ataccatttc ttcatttttg ttatttcgtt gtctctgttt ctgctgaaac ccgatacgct
     3001 gtaaatgata aaaactttgc taaacaaatc taaatacttt tcgaccgtgt ttttactgat
     3061 ttgcaaatca cgacccacac tttcgagtga taattcgctc cctgttctaa aagcgattat
     3121 ttgaagtaaa gcaattattt tgtctctttt tttgatgcct tcaaaggcaa aaatatctct
     3181 taacaattgg gtgttgacca attcttttaa gtaacttatt ttttcctcac gattcgtaat
     3241 atgactcaat tcaggataac tccctaatat taaacggtct tcgagattgc ttcgggtctg
     3301 tagtaaattt tcatttttag catattccat ttgtgctatt gggtataaat ttatggtttt
     3361 catacgtcca accaagggct ctcctaattg attattgatc tcaaatgcag aagagcccgt
     3421 aaccaaaatt tttaaatctt ggatagtatc taccatcaat tttagctttt ttccaatggc
     3481 aggtatttct tgtgcttcat caatgatcaa aaatttagta tcaccaagca atcttttaaa
     3541 atttgccgta ctctgaattt ctaataaact atgcgattca atatcgtctc cgttcaaaaa
     3601 aagtactttt tcatttagtt gctcaaccag cttttttata agttccgttt tgcccactcg
     3661 ccgtgctcct aaaagaacaa ttactttatt gggctgcagt ttttgcaaaa ttaaatcgag
     3721 tgccaatctg tctatttcca tgaatttaga ttttaaacaa tacaaatata tttaatttta
     3781 ttaaattccg tcaaaaaatt gacggaattt aattgatttc cgtcaaagta gtgacggaaa
     3841 tcaagtttaa gggattaaag tatagaaaat tgtaggattg aattctgtta gagtttcact
     3901 acttttgctc aatgataaat tggttcaaca atctgttttc aagaacagaa ataaccgaca
     3961 acaaagagta tatgaagaaa tttttaattg taggacttgg caacattggt gctgaatatg
     4021 tcaacacaag acacaatata ggttttaaag tagttgattt tctagctaag aaagaaggaa
     4081 tcagcttcga aacagtgaaa ctaggagcat tggccgaata caaattcaaa gggcgtactt
     4141 ttttgctctt gaagcccaat acctatatga atttgagcgg aaaagccgtt caatactgga
     4201 tggataaaga aaacattccg ctagaaaaca ttatggtcat taccgatgat ttgaatctat
     4261 ctttcggaac cattcgcatt cgaaaaaaag gtagtgatgg cggacacaac ggactcaaaa
     4321 acatcaatct tgttctgaat acgcaacaat acacccgttt tcgttttgga ataagcgacg
     4381 aattcaaaaa aggaaaacaa gtagattatg tcctaggaga atgggacgac actgaaaaag
     4441 cagcactacc agagcgatta gaattagctt cagaaattat aaaatcattt ggaaccgctg
     4501 gaatagaaaa tacaatgaca acatttaacg gaaaatgata aataattctg attttttgga
     4561 aatttaacct ccaagaaagc agagtttgcc taatttttgt caatttataa ttgcgatttg
     4621 gtaaaatcat taactttaac aaaatttttt aaaataatga aaacaacaac ccctctatct
     4681 atcaagttca tttttatatt tttttgtatg ctaactcttg gcaacattta cagtcaagac
     4741 aaaaatacaa caaccaaaaa aatttttgga caaactctta aatcatcaaa aaatccaaat
     4801 agcgataaga ttagatgcgt ttcaacagag tatgaacaat ttcttcaaga aaaaaatccc
     4861 aagagaatgt ccaatgctca attcgaaaca tggttggcac cgttggtttc taaatacaaa
     4921 gccacaaaag ccaattctaa aacagcagca acaataatta caattccagt ggtagttcac
     4981 gtagtacaca acggtcaaac cgttggaata gctccaaata ttaccgatgc ccaagttcaa
     5041 tctcagataa ctgtacttaa tcaggatttt agaagaatgc ttggtactcc gggctacaac
     5101 acaaatcccg taggagcaga tgttgaaatc gagtttgttc ttgctcagca agatcccagc
     5161 ggaaatccga caaatggaat tgaacgagtg agttttttgc agtcttcatg gtctgatacc
     5221 gacattgaaa atatcctaaa gcctgccact atttgggacc caacaatgta tttaaacatg
     5281 tggagtctta aatttactga caataccgtt ttgggttatg cacaatttcc tgatggctct
     5341 ggattagctg gacttaactc ttctggcgga agcgctaaca ctgacggagt tgtttccagt
     5401 tacgatgttt ttggaagcaa agactatggc agttcctttc tattagttac ttctttatat
     5461 aacaaaggga gaactatgtc gcatgaagtg ggacattggc ttggactatt acacatatgg
     5521 ggagacggag gtagtcgtga caataatacc aaagactgca atgcctccga ctattgtgca
     5581 gatactccac aagcaggatg ggaaaattat gactgtaata cttatgattc ttgtccagca
     5641 aatctaggaa atgatatggt agagaattat atggactata caaatgatga ttgtatgaac
     5701 atatttactc aaaatcaaaa agatcgaata gcggttatca tgaataatgc cgccagaaga
     5761 agcactttaa aaacatcaac aaaggatgat gcaatgtcat tgtttgccac tgatgccgaa
     5821 gttaaaatac tgagcttcaa tagcaatatt gattatggtt gcgctactcc accagcacca
     5881 acaaacaaac aagtgtctat ttataataga ggaacctcta ctttaacttc tgtaacttta
     5941 aattacaaca taaacggagg ctccaaccaa acctatactt ggagcggttc attaacacaa
     6001 aaccaatttg ccgacataac acttcccaac actaatttga atggcacttt aaatgttagc
     6061 atagcaacat ccaacggagg aacagaccaa agaactacta ataatactgc cagcaagaat
     6121 tattttaaca acgtacccac taactataat tatactaatt atgtctttag attacaacaa
     6181 gatttatggg gaagcgaaac tacttggagt ttaaaaaata gttctggtac cactttatat
     6241 agtggtggtc catatgaaga taagccttct ttacctttac cctcattaat aaccgaaaat
     6301 tggacattgc cttctaacca atgttatact tttacaataa atgactctca aagtgatgga
     6361 atttgttgcg gaaccgatgg aaatggctat tatgatatta agactaacaa tggcgctatt
     6421 accgttaaat ctggatcttc attttcatca agcgaaagca taacaattac aaacaacacc
     6481 ttaggaactg atacattcga ggcattaaat gaaatatatc tgtatccaaa ccctacaaaa
     6541 gggatt
//
LOCUS       DAOSNN010000149         6545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11868_length_6545_cov_4.325116, whole genome shotgun sequence.
ACCESSION   DAOSNN010000149 DAOSNN010000000
VERSION     DAOSNN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11868_length_6545_cov_4.325116"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLQ70_08225"
     CDS             complement(<1..214)
                     /locus_tag="PLQ70_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQE34638.1"
                     /translation="MQIKPTIISGTSAGAVIAAMYAFGKTPQEILEFFKSIYFFNWRH
                     FTFSKAGIIDSNAFKSYFHAIFDDALI"
     gene            111..773
                     /locus_tag="PLQ70_08230"
     CDS             111..773
                     /locus_tag="PLQ70_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34639.1"
                     /translation="MKNSRISCGVLPKAYIAAITAPALVPLIIVGFICTLIPTLITLW
                     LNERVKGSVKNSFDEKLSQFQIELNHLKTKENFKFTKLHEKRFEVLQESYKYLNNNLN
                     LLGKHISPFKFTPDGEDYDENEQKASKEYRDAHNEFLQYFNFNSIYFDEETETLLLSF
                     FQESGEIFNAYDQRQIYKSMGEKLEREDRLASAFAYKKIPEIIHPLKKEIEAKFRKLL
                     GD"
     gene            806..1351
                     /locus_tag="PLQ70_08235"
     CDS             806..1351
                     /locus_tag="PLQ70_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HQE34640.1"
                     /translation="MTTHLALLRGINVSGHNMIKMEVLKTTLEAIGFQNVQTYIQSGN
                     VFVDTEEENPAAVGFKIKQEIFKAFGHEVPVVVIGKSDLEACLKNNPFLKEADLDIKK
                     LYVAFVSTSLRSDSINDLKMSQVKPDEASIDANRVYIKYAVGAGKTRFDQKYIEKKLN
                     VTATIRNWNTVTQLLKLYEER"
     gene            complement(1420..1941)
                     /locus_tag="PLQ70_08240"
     CDS             complement(1420..1941)
                     /locus_tag="PLQ70_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34641.1"
                     /translation="MDSTRIIELISYSLPTIIMAFVAYSFFELYTKNENAKRNYLLQK
                     ESKPDTLSLRLQAYERMTLFLERINPSQLLVRISPISENKTDYQNFVIAQIEQEYEHN
                     LAQQIYVSNECWSTITTAKNATIQMILLAAKSEKISDAHQLRELILNDLLTKPSPSSI
                     ALEFLKNEVSQLW"
     gene            2035..2802
                     /locus_tag="PLQ70_08245"
     CDS             2035..2802
                     /locus_tag="PLQ70_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HQE34642.1"
                     /translation="MESYIIILLCLASFFAGFIDAVVGGGGLIQTPVALILLPNVSVS
                     SIIGTLKIPAFSGTSFAAYQYLKKVDMNWRLLAIMMLLAFPSAFLGSTLLTYVSNDFM
                     KPLLLVVLSLLVVYTYAQKNFGQHIEKSHSSQTQILYAVLISFVVGFYDGFIGPGTGS
                     FLVLGFIALMGFDFLHASANAKMVNLATNFGSICLFMLKGKIIWAVAIPMAFCNAFGG
                     WIGAKLAINKGNKFIRIFFLFVVVGTLIRFAYDVFIK"
     gene            complement(2815..3189)
                     /locus_tag="PLQ70_08250"
     CDS             complement(2815..3189)
                     /locus_tag="PLQ70_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HQE34643.1"
                     /translation="MKFVHRFAYYLLGLIMGSFVVAAIFSGKDTRCNYFPNARVLNDL
                     RTKPFYYSKQASQVLAEKWIDTTDIRNCLKYGDVDFDKSNIAVGNGKLYVIEGKTVKN
                     QEVTLNVINYSEKAVLDKIIKR"
     gene            complement(3225..4424)
                     /locus_tag="PLQ70_08255"
     CDS             complement(3225..4424)
                     /locus_tag="PLQ70_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HQE34644.1"
                     /translation="MTMAVSPFTKQQLLPQEEKLEIARHKSELFIGIPKETSYQERRI
                     CLTPDAVNSLTYQGHRVMIEAGAGESSSYSDKEYSQAGAEITNDTKKVFGCPIILKVE
                     PVTLAEIELISTNSIIISAIQLKTRQKSYFEALAKKKITALAIEYIKDDDGSYPILKS
                     LSEIAGTASILIAAELMITNEFGKGLLFGNITGVAPTDVVILGAGTVGEFAAKTALGL
                     GANVKVFDNSIRKLRCLQNNLNQRIFTSTIQTKSLLKALRRCDVAIGAMRGKERCPVI
                     VTETMVEHMKRGAVIVDVSIDSGGCFETSEVTSHEKPTFIKNNVLHYCVPNIPSRYSK
                     TASLSISNIITPYLLQIAEDGGIESAIRCNNGLKNGVYLYHGIHTNKAIGDWFNLPNS
                     DINLIVF"
     gene            complement(4475..4882)
                     /gene="tsaE"
                     /locus_tag="PLQ70_08260"
     CDS             complement(4475..4882)
                     /gene="tsaE"
                     /locus_tag="PLQ70_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQE34645.1"
                     /translation="MNITFSLDEINEVAQQILDQNPNKVILFHGEMGAGKTTLIKALA
                     KKLGVNDVTSSPTFSLVNEYQNTENQTIYHFDFYRLKTEIEALDMGADEYLYSGNWCF
                     IEWAEKIPSLIPDSHSIITISIAEDNKRFLELS"
     gene            complement(4958..6511)
                     /locus_tag="PLQ70_08265"
     CDS             complement(4958..6511)
                     /locus_tag="PLQ70_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HQE34646.1"
                     /translation="MDKIKILWVDDEIDYLKPHILFLEKKNYSVTTCNNGRDAIDIFD
                     NENFDIVFLDENMPGMSGLETLSEMKEKKSSIPMIMITKSEEEYIMEEAIGSKIADYL
                     IKPVNPNQILLSLKKNLDHSRLVSQKTTLDYQKEFRKISMEMAMVNSYEDWIELYKKL
                     LFWELELENIDDQAMIEILESQKAEANSQFGKFIERNYEDWFAPKADKPIQSHTLFRE
                     LVVPEIIKKDKPILFVVIDNLRYDQWKVFESVVGNHYKLEREVPYYAILPTATQYARN
                     AIFSGLTPLEMEKQFPQYWKNDPEEGGKNLFEAEFLTTQLKKLGLNIKQDYFKITNLA
                     GGKKLVENFKALKNNDLVTIVYNFVDMLSHAKTEMDVVKELASDDKAYRSLTLSWFKN
                     SPLLEIIQHAQKLGFKLILTTDHGTINVKNPSKVIGDKNTSLNLRYKTGRSLSYESKD
                     VYAVKEPKNIGLPAINMSSSYIFAKNDLFLAYVNNYNHYVSYYKNTYQHGGISLEEMI
                     IPFLIFNPK"
BASE COUNT         2052 a         1071 c         1097 g         2325 t
ORIGIN      
        1 caattaaagc atcatcaaaa atagcatgaa aataactttt gaaagcattt gaatcaataa
       61 ttccagcttt tgaaaatgta aaatgcctcc aattaaaaaa gtaaatcgat ttgaaaaatt
      121 ctagaatttc ttgtggagtt ttgccaaaag catacatagc agcaattact gcacctgcac
      181 ttgtaccact aattatagta ggttttattt gcactttaat accaacatta attacacttt
      241 ggttgaatga aagggtaaaa ggtagtgtta aaaattcttt tgatgaaaaa ctttctcaat
      301 ttcaaattga acttaatcat ttaaaaacaa aagaaaattt taagtttaca aaactccacg
      361 aaaaacgatt tgaagtttta caagaatcat ataaatattt gaacaataat ttaaatttat
      421 taggaaagca tatttcacca tttaagttta caccagatgg tgaagattat gatgaaaatg
      481 agcaaaaagc cagtaaggaa tatcgtgatg ctcacaacga atttttgcag tatttcaact
      541 ttaattcaat ttacttcgat gaagaaacgg agaccttgtt acttagcttc tttcaggaat
      601 ctggtgaaat atttaatgct tatgaccaaa gacaaattta taaatcgatg ggtgaaaaat
      661 tagaacgaga agatagatta gcctcagctt ttgcctacaa gaaaatccct gaaataattc
      721 atcctttaaa aaaagaaatt gaagctaagt ttcgtaaact tttaggcgac taattttatt
      781 tattttcata attaattaaa aaataatgac aacccatctc gcactgcttc gtggcatcaa
      841 tgtttctggt cacaatatga taaaaatgga ggttttgaaa accactttgg aagctattgg
      901 ttttcagaat gtgcaaacct atattcaatc gggtaatgtt tttgtcgata ctgaggagga
      961 aaatcctgcc gctgtgggtt tcaaaatcaa gcaggaaatt ttcaaggctt ttggtcacga
     1021 agttcccgtt gtagttattg ggaaatctga tttagaagct tgcttgaaga acaatccttt
     1081 tctgaaagag gcagatttag acattaaaaa attgtacgta gcctttgttt ccacaagctt
     1141 gcgaagtgac agtatcaatg atctgaaaat gagtcaggtt aaacccgacg aagctagtat
     1201 tgatgccaac agggtttaca taaaatatgc tgttggagca ggaaaaacaa gatttgacca
     1261 gaaatatatc gagaaaaaat tgaacgtaac agcaaccatt cgcaattgga ataccgtaac
     1321 gcaattattg aaactatacg aggaacgata atttcatgat aaaatcagtt taatccaata
     1381 atctttggct aaaaaaacag gagcgaagcg acttttacac taccacaact ggctaacttc
     1441 gtttttgagg aattctaatg caatactact tggagatggt tttgtcaata aatcgtttag
     1501 aattaattct cgtaactgat gtgcatcact aattttttcg cttttagcag ccaaaagaat
     1561 catttgaatg gtagcgtttt tagctgtagt tattgtggac caacattcat tcgaaacata
     1621 aatttgttgt gctaaattgt gttcgtattc ttgttcgatt tgcgcaatta caaagttttg
     1681 ataatctgtt ttgttttctg aaattggaga aattcgaacc aatagttgcg atgggttgat
     1741 acgttccaaa aacaatgtca ttcgctcgta ggcctgtaaa cgtagtgata aagtatctgg
     1801 ttttgattct ttttgcaata aatagtttct ttttgcattc tcatttttgg tgtaaagttc
     1861 gaagaaacta tacgccacaa acgccattat tattgttggt aatgaataac ttatcaattc
     1921 tattattcgt gttgaatcca tttgaaattt gattttttta atttttttta aacgaagaca
     1981 aattaacgaa taaagccata aacttgtagt acatttgatg aaattttata aaaaatggaa
     2041 tcttacatta ttatcctgct ttgtttagcc tcattttttg caggatttat tgatgcagtt
     2101 gtaggtggtg gtggattgat tcaaactccg gtagctttga ttttgctgcc aaatgtatcg
     2161 gtttcctcca ttattggaac cttaaaaata cctgctttta gtggcacttc ttttgcagca
     2221 tatcaatact taaaaaaagt ggatatgaac tggagattac tagctattat gatgcttttg
     2281 gcgtttccat cggctttttt gggttcgact ttgttgactt atgtaagtaa tgattttatg
     2341 aaacctttat tgctagttgt cctttcgctt ttggttgttt acacttatgc acagaaaaat
     2401 tttggacaac atattgagaa aagtcattct tcccaaactc agattcttta tgccgtactt
     2461 atcagttttg tggtgggttt ttatgatggt tttataggcc caggaacagg aagtttcttg
     2521 gttttgggtt ttattgcctt gatgggattc gattttttgc acgcttctgc taatgccaaa
     2581 atggtgaatc tagcaactaa ttttggttct atttgcttgt ttatgttgaa aggtaaaatc
     2641 atctgggcag ttgcaattcc aatggctttt tgcaatgctt ttggtggttg gattggtgcc
     2701 aaattagcaa tcaataaagg aaataaattt attcgaattt tctttttgtt tgtagttgtt
     2761 ggaactttaa ttaggtttgc ctacgatgtt tttattaaat aaattcgacg ctaattatct
     2821 ttttataatt ttatctaaaa ctgctttttc agaataatta atcacgttta atgtcacttc
     2881 ttggtttttt actgttttac cttcaataac atagagtttt ccatttccta cagcaatatt
     2941 gcttttgtca aaatctacat caccatattt caaacagttt ctaatgtcag ttgtgtctat
     3001 ccatttttca gctaatactt gtgaagcttg cttagaataa taaaaaggtt tggttcttaa
     3061 atcattcaat acacgagcat ttggaaaata attgcaacga gtatcttttc cgctaaatat
     3121 agcagcaaca acaaaagatc ccattattag acccaataaa tagtaagcaa agcggtgtac
     3181 gaatttcata ttttaatttt cggcaaatat aaaggaaagt ttaattaaaa cacgattaaa
     3241 tttatatcgc tattaggcaa attaaaccaa tcaccgatgg ccttgttagt atggattccg
     3301 tggtataaat acactccatt ttttaaacca ttattgcatc tgatagcact ttctattcct
     3361 ccatcttcgg caatttgtaa caaataagga gtgatgatat tactgataga aagcgaggct
     3421 gttttagaat atcgagaagg aatgttggga acacaatagt gcaaaacatt atttttgatg
     3481 aaggttggct tttcatgcga agttacttcc gaggtttcga aacatcctcc agaatcaatg
     3541 ctcacatcaa caattacagc acctcttttc atgtgttcga ccatagtttc ggtaacaatt
     3601 acagggcaac gttccttgcc acgcattgcg ccaatagcta catcacatct tcttagggct
     3661 tttaataacg acttggtttg aatggtcgaa gtgaatattc gttggttcaa attattttgc
     3721 aaacagcgca attttctaat agaattgtca aaaactttca catttgcgcc taagcccaaa
     3781 gctgtttttg cggcaaactc tccaacagtt ccagctccaa gaataaccac atcagtagga
     3841 gctacgcctg taatatttcc gaataataac cctttgccaa attcattggt aatcattaat
     3901 tcggcagcaa ttaaaataga agctgttcct gcaatttcac tcaaagattt caaaatagga
     3961 taggagccat cgtcgtcttt tatatattcg atagcgagag ctgttatttt tttcttagct
     4021 aaagcttcaa aatacgattt ctgtcttgtt tttaattgaa tggctgaaat tatgattgaa
     4081 tttgtactga tcaattctat ttcggctaaa gttactggct cgactttcaa aattatcgga
     4141 caaccaaaaa ctttttttgt atcattagta atttcggcac ctgcttgtga atattccttg
     4201 tcagaataac ttgaactttc tcctgcacca gcttctatca ttacgcgatg tccttgataa
     4261 gtcaatgaat ttacggcatc gggtgtcaag caaatgcgac gttcttgata gcttgtttct
     4321 ttcggaatgc ctataaagag ttcgcttttg tgtcttgcta tttcaagttt ttcttcttga
     4381 ggtaataatt gttgttttgt aaatggagat actgccattg taatgttaaa ttagtggtac
     4441 aatttacaga aaaaattaga attaattttt tttattagct taattctaaa aaacgcttgt
     4501 tgtcttctgc tatagaaatt gtaataatcg aatgggaatc aggaataaga ctaggaattt
     4561 tttctgccca ttcaataaaa caccaatttc cagaataaag gtattcgtct gctcccatat
     4621 ccaaagcttc gatttcagtt tttaatcgat aaaaatcaaa atgataaatt gtttgatttt
     4681 cagtgttttg atattcgtta actaaagaga aagtagggct gcttgtgacg tcatttacac
     4741 ccaatttttt ggctaaagct ttaattaaag ttgtttttcc agctcccatt tcgccgtgaa
     4801 aaagaatcac tttgttggga ttttgatcta aaatttgctg ggcaacttca ttaatttcgt
     4861 ctaatgaaaa agtgatattc attctgtata agtttgtggt tggttgttta gggtttagag
     4921 ttagtaacaa attataaacc ataaacccca aactgttcta ttttgggtta aaaatcaaaa
     4981 acggaataat catttcttct aatgaaattc ctccgtgttg ataagtgttt ttataataac
     5041 tcacataatg attgtaattg ttcacatagg ctaaaaacaa atcatttttt gcaaaaatat
     5101 aagaactact catatttata gcaggtaaac cgatattttt tggttcttta accgcataaa
     5161 catctttgct ttcgtaggat aaactgcgtc ctgttttata tctaagattt agacttgtat
     5221 tcttatcgcc aatcactttc gaaggatttt ttacattgat cgtgccgtga tcggtagtca
     5281 atattagttt aaaacccagt ttttgagcat gttgaataat ttctaaaaga ggcgaatttt
     5341 tgaaccaact taaagttaaa gaacgataag ctttatcgtc agaagcgagt tctttgacca
     5401 cgtccatttc ggttttggcg tgagaaagca tatcgacaaa attataaaca atcgtaacca
     5461 aatcgttgtt ttttaaagct ttgaagtttt ctaccaattt ttttccgcca gcaagattgg
     5521 ttattttaaa ataatcttgc ttgatgttta atcctaattt tttgagttga gtggtcagga
     5581 attctgcctc aaacaagttt tttccgcctt cttcgggatc atttttccaa tattgtggaa
     5641 actgtttttc catttctagc ggtgttaatc cagagaaaat agcgtttcga gcatactgag
     5701 tggctgttgg caaaatagcg taatacggca cttctctttc taatttataa tgattaccga
     5761 ctacactttc aaatactttc cattggtcgt aacgcaaatt atcaattacg acaaacaaaa
     5821 tgggtttgtc ttttttgatg atttcaggaa ccactaattc acgaaacaaa gtatgagatt
     5881 gtatgggttt atctgctttt ggagcaaacc aatcttcgta attgcgctca atgaattttc
     5941 cgaattgcga attggcctct gctttttgcg attccaatat ttcaatcata gcttgatcgt
     6001 caatattttc tagttccaat tcccaaaaca acagcttttt gtataattca atccaatctt
     6061 cataggaatt gaccatagcc atttccatcg aaattttgcg aaattctttt tggtaatcca
     6121 aagtggtctt ttgcgaaacc agtctagaat ggtctaaatt ctttttcaaa ctcaacaaaa
     6181 tctgatttgg gtttaccggt tttatcaaat aatcggcaat ttttgaacca atcgcttcct
     6241 ccataatata ctcttcttcg ctcttggtaa tcattatcat cggaatagaa gatttttttt
     6301 ccttcatttc cgaaagggtt tccaagccgc tcattccggg catattttcg tccaaaaaga
     6361 caatatcaaa attttcgtta tcaaaaatat caatggcgtc acgtccgtta ttgcaagtgg
     6421 tgacactgta atttttcttt tccagaaata aaatatgtgg ttttaagtag tcaatttcgt
     6481 catcgaccca aagtattttt atcttgtcca taattgtaag taaaaagtat tcaaaagtac
     6541 taaaa
//
LOCUS       DAOSNN010000150         6517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_11922_length_6517_cov_5.320334, whole genome shotgun sequence.
ACCESSION   DAOSNN010000150 DAOSNN010000000
VERSION     DAOSNN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11922_length_6517_cov_5.320334"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2409)
                     /locus_tag="PLQ70_08270"
     CDS             complement(<1..2409)
                     /locus_tag="PLQ70_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQE34647.1"
                     /translation="MNINKFTIKSQEAIQHSQQLAQSFGQQQIENEHIFKAIFEVDEN
                     VAPFILKKLNVNVPLFQQILDSTIQSFPKVSGGEIMLSRTTNTTLNEAEIIAKKMNDE
                     FVSIEHLILAIFDSKSKVSQILKDQGVTGKGLKAAIEELRKGERVTSASAEETYNSLN
                     KYAKNLNDLAKNGKLDPVIGRDEEIRRVLQILTRRTKNNPMLIGEPGVGKTAIAEGLA
                     HRIVDGDVPENLKDKIVFSLDMGALIAGAKYKGEFEERLKSVVKEVTAAEGDIVLFID
                     EIHTLVGAGGGEGAMDAANILKPALARGELRAIGATTLDEYQKYFEKDKALERRFQKV
                     IIEEPDTESAVSILRGIKEKYETHHKVQIKDEAIIAAVELSQRYISNRFLPDKAIDLM
                     DEAASKIRMEINSKPEELDVLDRKIMQLEIEIEAIKREKDESKLKILGMDLANLKDER
                     NEIFTKWKSEKDVVDNIQTVKTEIEDFKYEAERAERDGDYGKVAEIRYGKIKEAQERL
                     DVLHKELIENQAGGSSLIKEEVTREDIAEVVAKWTGIPVTKMLQGEREKLLHLEDELH
                     RRVVGQEEAIEAVSDAVRRSRAGLQDMKKPVGTFLFLGTTGVGKTELAKALAEYLFDD
                     ENAMTRIDMSEYQERHSVSRLVGAPPGYVGYDEGGQLTEAVRRKPYSVILLDEIEKAH
                     PDTFNILLQVLDEGRLTDNKGRLADFKNTIIIMTSNMGSQIIQEKFENLKGSVEAATE
                     AAKVEVLGLLKQTVRPEFINRIDEIVMFTPLTNANIAQIVGLQLKSVTKMLAQQGITM
                     DATPE"
     gene            2564..2953
                     /gene="ytxJ"
                     /locus_tag="PLQ70_08275"
     CDS             2564..2953
                     /gene="ytxJ"
                     /locus_tag="PLQ70_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HQE34648.1"
                     /translation="MSVFSNLFGSSEKQDNSNSKINWTPLTYMGQLDEMVAFSEQKPA
                     VIFKHSTRCSISRFALKQFEKDFDLEDKVDAYFLDLLEHRDISNEIASRFGVYHQSPQ
                     LLLIKEGKSVYDVSHEGINAGVLKGKL"
     gene            2990..4591
                     /locus_tag="PLQ70_08280"
     CDS             2990..4591
                     /locus_tag="PLQ70_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQE34649.1"
                     /translation="MKSFFSILIITLSFNSFGQNLIPNYQIGSFIDYNKKIINGYYDF
                     NYSPKISLKVSYESNENFAQGFYFDVNGLKVNGLLKYSLSDRDLKFKLNEADIEKSIK
                     ADETNGYIIGIDTFSVVKNVEIIGLFGGKTSKKGEFAANIENIGGMKFYKFTANGPNG
                     NTYNSYIVKKSESSDFVTFPSGSAKFKSVAFDVFENDATLKEYIEKGKYKADDIPSIL
                     KIYKYRKLFKNGQKIDYNSSFDEMNNKDEVAYYSKIESIQDSVFHLTHFFNNNLKIYD
                     GDFTSFYPHNKQGDFKFYFPNGEVRRKISFDNNKPKSGIEYFENGKIHRVYQILEQGS
                     LVYREVYGDSNVNLLDNKCTGSEEYTDPISEKKITYEYENKKLKTAYFVDENGEKVYQ
                     LCENNAEIRKFNSLQKAVKEELKFPSESLQKNVHGFVLVKCIVEPSGLVSELKIIKGL
                     DEACNKATLDFLSRFKTENYWNPGKVDGKEVKQEIVIPIDFSIVNAAIYRNNYYNFWW
                     QNNMMMQQQMMMQQQNNMMRSAGFR"
     gene            complement(4687..5967)
                     /gene="argH"
                     /locus_tag="PLQ70_08285"
     CDS             complement(4687..5967)
                     /gene="argH"
                     /locus_tag="PLQ70_08285"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQE34650.1"
                     /translation="MKLWEKGIPTDKQIEHFTVGNDRELDLVLAKYDALGSIAHAKML
                     CQIGLLTEEETDLLVLALEDIIIDAEKGEFEIEDSFEDVHSKIEYLLTIKLGDAGKKI
                     HTARSRNDQVLVDVNLYLKDVVTEFKSQVKTLFDLLMESADKYQNVLLPGYTHLQIAM
                     PSSFGMWFSAYAETLIDDITMLNASLKVVDQNPLGSAAGYGSSFPINRTFTTQELGFE
                     TLKYNSVAAQMSRGKSEKTVAFAMSSVAATLAKLSMDVCLYMSQNFDFITLPAHLTTG
                     SSIMPHKKNPDVFELIRGKCNKIQALPYEITLITNNLPSGYHRDFQLLKEGLFPAIQN
                     LKACLDIAIFSIKDIKVKEGILNDKKYDYLFTVDTLNEMVVSGMPFRDAYKAVAEQLE
                     NGTYQSPKETKHTHEGSINNLCLEEIKEKMKLAF"
     gene            complement(6050..>6517)
                     /locus_tag="PLQ70_08290"
     CDS             complement(6050..>6517)
                     /locus_tag="PLQ70_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HQE34651.1"
                     /translation="PVIEWFKTYQFEKISEVLGPVKMTVTQVNAGKQHNVVPAECHLV
                     VDIRVNDCYNNTEILETVRANVNAEVTPRSMHLNASSIPVNHGLVQAGIALGRTTYGS
                     PTLSDQSVLYCKSLKLGPGESLRSHSADEFIYVNEIEEGIQLYIKILGDFLKQ"
BASE COUNT         1897 a         1192 c         1244 g         2184 t
ORIGIN      
        1 ttctggcgtg gcgtccattg tgatgccttg ttgtgccagc attttggtca ccgatttcag
       61 ttgcaagcct acgatttggg caatattggc attggtcaaa ggcgtaaaca tcacgatttc
      121 gtcaatacgg ttgatgaatt cgggacgaac ggtttgtttc aataagccca gaacttccac
      181 tttggcagct tcggtggcgg cttcgacact tcctttcaaa ttctcgaatt tctcttgtat
      241 gatctggctt cccatattag aagtcataat gataatcgtg ttcttgaaat cggcgagacg
      301 tcctttgttg tccgtcaaac gtccttcgtc caagacctgc agcaagatgt tgaaagtgtc
      361 gggatgcgct ttttctatct cgtccaacaa aatcacagaa tagggtttac ggcgcacggc
      421 ttctgtcaat tgaccgcctt cgtcatagcc cacataacct ggaggcgcac ccaccaaacg
      481 gctcacgctg tggcgttctt ggtattcgct catatcaatt cgggtcattg cgttttcgtc
      541 gtcgaacaag tattctgcca aggctttggc caattcggtt tttccaacac ctgttgttcc
      601 caagaaaagg aaagtcccaa ccggtttttt catatcctgc aaaccggcac gagaacgacg
      661 tacggcatcg ctaacagctt cgatggcttc ttcctgacct acaactctgc ggtgcaattc
      721 gtcttctaga tgcaagagtt tttctctttc gccttgcaac attttggtta cgggaattcc
      781 agtccatttg gctacgactt cggcaatatc ttctcgggtc acttcttctt taatcaaaga
      841 actgccacca gcttgatttt cgattaattc tttatgtaaa acgtccaagc gttcttgggc
      901 ttccttgatt tttccgtaac gaatttcggc tacttttccg taatcgccat cacgttcggc
      961 acgttcggct tcgtatttaa agtcctcgat ttcggtttta acggtttgaa tgttgtccac
     1021 cacgtccttc tcggatttcc atttggtaaa gatttcattt cgctcgtctt taagattcgc
     1081 caaatccatt cctaaaattt tgagtttgga ttcgtctttt tctcttttaa tagcctcaat
     1141 ttcgatttcc aattgcatta tttttcgatc caaaacatcc aattcttcgg gttttgaatt
     1201 gatttccata cgaattttcg aagccgcttc gtccatcaaa tcgatggctt tgtctggcaa
     1261 aaatcggttg ctaatgtagc gttgcgataa ttcgacagcg gcaataatgg cttcgtcttt
     1321 aatctgcact ttatgatgcg tttcgtattt ttccttgatt cctcgaagta tagatactgc
     1381 actttcggta tcgggttcct caattattac tttttggaaa cgacgttcta aagctttgtc
     1441 tttttcgaaa tatttttggt attcgtctaa agtcgttgca ccaatggcac gcagttcgcc
     1501 acgagccaaa gcaggtttca gaatattcgc cgcatccatt gcaccttcgc caccgccagc
     1561 accaactaac gtatggattt cgtcaatgaa aagcacaata tcgccctctg cagcagtcac
     1621 ttctttcacg accgatttca aacgttcttc gaactctcct ttgtatttag ctccagcaat
     1681 caaagcgccc atatccaaag agaaaacaat tttgtctttc aggttttcgg gaacgtcgcc
     1741 atctacaatt cggtgcgcca aaccttcggc aatggcggtt ttaccaactc caggttctcc
     1801 gatcaacatc gggttgtttt tggttcttcg agtcaagatt tgtaatactc tacggatttc
     1861 ttcatcacgc ccaataacgg gatcgagttt gccatttttg gctaaatcgt tcaggttttt
     1921 ggcgtatttg ttcaaagaat tataggtttc ttcggccgat gcagaagtca ctctttcacc
     1981 tttacgcaat tcttcgatgg cggctttcaa tccttttcca gttacgcctt ggtctttcag
     2041 gatttgagaa actttacttt tagaatcgaa aatagccaag attaaatgct cgatagaaac
     2101 gaattcatcg ttcatctttt tggcaatgat ttcggcttcg ttcaaggtgg tgtttgtcgt
     2161 tctggaaagc ataatttcgc cacccgaaac ttttggaaag ctttggatgg tactgtctaa
     2221 aatttgttgg aacaacggaa cgttgacgtt tagttttttc aaaatgaaag gtgctacatt
     2281 ttcatccact tcaaaaatgg ctttgaaaat gtgttcgttt tctatttgtt gttgtccaaa
     2341 actttgagcc aattgttgcg aatgctgaat ggcttcttgt gatttgatgg taaatttatt
     2401 gatgttcatt tttttgattg tttgttgttt atagtttttt gtttgtagtt atttaactaa
     2461 cttcggcaaa taatcgtcaa atcttattcc accactaaag ttacgacatt ttgactgtaa
     2521 ataagggggt tagaggcgac aaaatgactt aaaaagcgac aaaatgagtg tgttttcaaa
     2581 tcttttcggc agttccgaaa aacaagataa ttctaatagt aaaattaatt ggactccgtt
     2641 gacttatatg ggacagttgg atgaaatggt tgccttttca gagcagaaac cagccgttat
     2701 tttcaaacac agtacccgtt gcagcatcag cagatttgcc ttgaaacaat ttgaaaaaga
     2761 cttcgattta gaagataaag ttgatgcata tttccttgat ttattggaac acagagatat
     2821 ttcgaatgaa attgccagtc gttttggagt gtaccatcaa tcgccacaat tgttgttgat
     2881 taaagaagga aaatcggttt atgatgtttc gcacgaggga attaatgccg gggtattgaa
     2941 agggaagtta tagttttttt aatgttttta ttctaatatt tttttaccaa tgaaaagttt
     3001 tttttctatt ttaattatta ccctttcttt taactctttc ggacagaatt taattcccaa
     3061 ttatcaaata ggaagtttta ttgactacaa caaaaaaata atcaacggat attacgattt
     3121 taattacagc cccaaaattt cacttaaagt gagctatgaa tcaaatgaga attttgctca
     3181 aggtttttat tttgatgtta atggtttaaa agtgaatggt ttactgaaat actcgctaag
     3241 tgatagagat ttgaagttta aattgaatga agcggatatt gaaaaatcaa taaaagctga
     3301 tgaaacaaat ggctatatca ttggtattga tactttttct gttgtaaaaa atgttgaaat
     3361 aattggtctt ttcgggggta aaacttcaaa aaaaggagag tttgctgcca atattgagaa
     3421 tattggagga atgaagtttt ataaatttac agcaaatggt ccaaatggga atacgtataa
     3481 tagttatatt gtaaaaaaat ctgagtcttc tgattttgtg acattcccat caggtagtgc
     3541 taagtttaaa agtgttgctt ttgatgtttt tgaaaatgat gctactctta aggaatatat
     3601 agagaaaggt aaatataaag cagatgatat tccttctatt ctaaagatat acaaataccg
     3661 aaaactgttt aaaaacggac aaaaaattga ttacaattct tcttttgatg aaatgaacaa
     3721 taaggatgaa gttgcttatt attcaaaaat agaatcaatt caggattctg tttttcattt
     3781 aacgcatttt ttcaataaca acttgaaaat ttatgatggt gacttcacct ctttttatcc
     3841 acataataaa cagggtgatt ttaaatttta tttccctaat ggagaagtta gaagaaaaat
     3901 tagctttgat aataacaaac caaaatcagg aattgaatac tttgaaaatg gtaaaattca
     3961 tagagtttat caaattttag agcaggggtc attagtttat agagaagtat atggcgactc
     4021 taatgtaaat ttgttggata ataaatgtac tggaagtgag gaatatactg atcctatttc
     4081 agaaaaaaaa atcacctatg agtacgaaaa taaaaagctt aaaacggctt attttgtaga
     4141 tgaaaatgga gaaaaagtat atcagctctg tgaaaataat gctgaaatta gaaaatttaa
     4201 tagtttgcaa aaggcggtta aagaggaatt aaaatttcct tctgaatctt tgcaaaagaa
     4261 tgttcacggt tttgtattag ttaaatgtat cgttgagcca tcaggattgg tttctgaatt
     4321 aaaaattatt aaagggttag atgaggcttg taataaagca actttagatt ttttgagtcg
     4381 ttttaaaaca gaaaactatt ggaatcctgg gaaagttgat ggaaaagaag taaaacaaga
     4441 aattgttatt ccaatcgatt tttcgatagt aaacgcggct atttacagga ataattatta
     4501 caatttttgg tggcaaaata atatgatgat gcagcaacaa atgatgatgc aacaacaaaa
     4561 taatatgatg agatccgcag gatttagata gtgttaataa gattttgttc aaaaaagtaa
     4621 aaaaccgcaa attcacagat tatattaaaa tctacgaatc tgcggtttgt tttttggttt
     4681 aaaaccttaa aaagccaatt tcattttctc tttaatctcc tccaaacaaa ggttgttgat
     4741 gcttccttcg tgggtgtgtt tggtttcttt tggcgattga taagtgccgt tttccaattg
     4801 ttctgcaacg gctttgtagg catctctaaa cggcattcca gacacgacca tttcgtttaa
     4861 agtatcaact gtaaataggt aatcgtattt tttgtcgttt aatattcctt cttttacctt
     4921 gatgtctttg atggagaaaa tcgcaatatc caaacaagct ttcaagtttt gaatggctgg
     4981 aaacaaacct tctttcaaca attggaaatc tcggtgataa ccgcttggca agttgtttgt
     5041 aattaatgtg atttcataag gcaaagcttg aatcttgttg cattttccac gaatcaactc
     5101 aaaaacatcc gggtttttct tgtggggcat aatgctggaa cccgtcgtta aatgagcagg
     5161 caaagtgatg aaatcgaaat tttgactcat atacaaacaa acgtccatag agagtttggc
     5221 caaagtggcg gccacactac tcattgcaaa agctactgtt ttctccgatt ttccacggct
     5281 catttgagca gcaaccgaat tgtatttcaa ggtttcaaaa cccaattcct gtgttgtaaa
     5341 agttctgttg atggggaagg agcttccata ccctgcagcc gaacccaatg gattttgatc
     5401 gaccactttc aaggaggcat tcaacatcgt gatatcgtca atcaaggttt cggcataagc
     5461 ggaaaaccac atcccgaaag aagatggcat cgcaatttgc aaatgcgtat aacccggcaa
     5521 caatacgttt tggtatttat ctgccgattc cattaacaaa tcgaacagcg tttttacttg
     5581 cgatttgaat tcggttacca catcttttag atataaattc acgtcaacca aaacttggtc
     5641 gttacgagaa cgagcggtgt ggattttctt tccagcgtcg cctaatttta tagtcaaaag
     5701 gtattctatt ttggaatgca cgtcttcgaa actatcttca atttcgaatt cgcctttttc
     5761 ggcatcgata atgatgtctt ctaaagccaa aaccaataaa tcggtttctt cttccgttaa
     5821 aagtccgatt tgacacaaca ttttggcgtg agcgatagaa cccaaagcat cgtatttggc
     5881 caaaactaag tctaattcgc ggtcgtttcc aacggtgaaa tgttctattt gtttatctgt
     5941 tggtattcct ttttcccaaa gtttcatagt caattttaga ttttagattt tagattttag
     6001 attcaaaacg aatggatttg aaatttagaa atactaaaat ttattgtaat tattgtttaa
     6061 gaaaatcgcc aagtattttg atatacaatt ggattccttc ttctatttcg ttaacgtata
     6121 taaattcgtc cgccgagtgg gaacgcaagc tttctcccgg acccaatttc aatgatttgc
     6181 agtacaaaac ggattgatcg gaaagggtag gtgagccgta agtggttctt cccaaagcga
     6241 ttcctgcttg gaccaatccg tgatttacag gaatggatga agcattcaag tgcatcgaac
     6301 gcggtgtaac ttcagcattc acgttggctc ttacagtttc caaaatttcg gtattgttgt
     6361 agcaatcatt cacccgaata tcgacaacca aatggcattc ggccggcacg acattgtgct
     6421 gtttcccggc gttgacttgg gttacggtca tttttaccgg gcccaaaact tcggatattt
     6481 tctcgaattg ataggttttg aaccattcaa tcacggg
//
LOCUS       DAOSNN010000151         6481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12004_length_6481_cov_6.892624, whole genome shotgun sequence.
ACCESSION   DAOSNN010000151 DAOSNN010000000
VERSION     DAOSNN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12004_length_6481_cov_6.892624"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLQ70_08295"
     CDS             complement(<1..847)
                     /locus_tag="PLQ70_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34652.1"
                     /translation="MKNQIKNIGLLALATMIFASCTNNENLDPIPPTAAAFKGINEAG
                     LKSNTQNFTATAGTGTITLTSAKGVIITLNGNTLTKNGSPVTGAIDITFIELFDKGNM
                     LITNKPTMGVMADGKKSLLKSGGEFFIKASQGGVDLVSNGGIQLQVPANLTGTIDTGM
                     LFWAGDITTDPENLAWVRPGTAAGTGGQKDGNVDFKQNTYNVSFGNFGWTNVDKFYSD
                     PRPRTTILAEVPTGYDNTNSAIYLSYDGEGTNALAKLDTYNATTKQFSEHYGQIPIGL
                     ACHVIF"
     gene            complement(1022..1408)
                     /locus_tag="PLQ70_08300"
     CDS             complement(1022..1408)
                     /locus_tag="PLQ70_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34653.1"
                     /translation="MKSTHHKSEKNTINKYYLLMVLATSIGFAQTEKSTQDIVKAKSD
                     LASTGAITNPYFKENSMSGEMTSISDETTEPVHILKTRTKSNQTNERTTRPNGQDDGN
                     PFPPKSTLKQESSVKPGYNVKANKKI"
     gene            1537..2121
                     /locus_tag="PLQ70_08305"
     CDS             1537..2121
                     /locus_tag="PLQ70_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQE34654.1"
                     /translation="MAQSTIHPDQIYIDGLANNNSAVIQSIYKNFVPKVVSYIRNNSG
                     DQDQAQDVVQEIMILLFNQAKANKLQLTCPFDAYFFLLCKRKWLNELKKSSNKGVTID
                     DDLPSTNEPTEQMVTETELFDEKQQLFDLMFQKLGDKCQELLKLSFTIKSMEEVAEKL
                     NVTYGYVRKKKSLCVGQLTQWIQETNHFQSLKIT"
     gene            2124..2846
                     /locus_tag="PLQ70_08310"
     CDS             2124..2846
                     /locus_tag="PLQ70_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE34655.1"
                     /translation="MNEERYIKFDQYLANEMSAEEKTVFEKQLSEDPELATSLEVFKE
                     LHLHLENKFGKKAEFNAYKKNIESISKKHFKTKKSKVIDLRSWHYAVAASVTILFGLF
                     LFQNINPTFEDYDNPEMATFVERGDVNENLKLAQDAFNAKEYKKAIPIFETVLKTNKS
                     PEIQYFYSIALLEDNQLQKAEINFSDLRYGNSIYKNKATWYLALSKLKQKDFRSCKDI
                     LLTIPDDFEDYDQVQELLNELD"
     gene            2988..3755
                     /locus_tag="PLQ70_08315"
     CDS             2988..3755
                     /locus_tag="PLQ70_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQE34656.1"
                     /translation="MIITDTHTHLYSTEFDQDRNEMIQRAIDAGVTRFFIPAIDSAYT
                     QSMYDLEKAYPENIFLMMGLHPTHVKDNYLDELQHVEEELSKRKFFAVGEIGIDLYWD
                     KTHLPQQQDAFRKQIQLAKQYKLPIVIHCREAFDEIFEILEAEKSTDLFGIFHCFSGT
                     YEQALQAISYNMKLGIGGVVTFKNGKIDQFLSQIDLKHIVLETDSPYLAPVPFRGKRN
                     ESSYLINVIDKLSMIYNLSKEEIALITTQNSKAIFGI"
     gene            3817..4953
                     /locus_tag="PLQ70_08320"
     CDS             3817..4953
                     /locus_tag="PLQ70_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQE34657.1"
                     /translation="MSKFDAIRPFYETEVNDAIQGVLHHPMMKALMNFTFPDLPDEIW
                     KEQLLRTHSTRDFQCNFTYKSLKKILEKKSDGLTTSGFEKLQKNTPYLFISNHRDILL
                     DTSLLNACLFENKLVMTASAIGDNLVQKSFLRTLARLNRNFLVQRGLSPREMLQSSKL
                     LSEYISILLQHENRSIWIAQREGRTKDGNDATNPGVLKMLAMASDEENLMDYFKKIQI
                     VPVSISYEYDPTDFLKMPQLLAEAKQEIYIKEKNEDFINLMSGVMGQKKRIHIHVGDV
                     LDKELDIIKELHENSNKQIQALAQIIDDSILSNYKLWPTNYIAYDILNKTTTYQHLYT
                     ENEKSLFERRLEMRIDHSNPLALQGFLSMYANPVVNKLKYENAI"
     gene            4943..5971
                     /locus_tag="PLQ70_08325"
     CDS             4943..5971
                     /locus_tag="PLQ70_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HQE34658.1"
                     /translation="MLSKAKILLIYTGGTIGMMKDFETGALKAFNFRKLLQKIPELKQ
                     LDCDIETVSFEKPIDSSNMNPENWTEIAQIIENNFDNFDGFVVLHGSDTMSYSASALS
                     FMLENLSKPVIFTGSQLPIGDLRTDAKENLITAIQIASLKENDKPLISEVCLYFEYKL
                     YRGNRTTKINAENFNAFISPNYPTLIESGVNLKTFPDLFLPQTKAKKLKIHKKLDTNV
                     AIIKMFPGISETVLSAIFDIPQLKGIVLETYGAGNAPTEDWFLSLLKKAIANGLYIVN
                     VTQCSIGSVNMGKYETSTALKEIGVISGKDITTEAAITKLMYLLGQNVSKENFKTLFE
                     TSLRGEIS"
     gene            6033..6119
                     /locus_tag="PLQ70_08330"
     tRNA            6033..6119
                     /locus_tag="PLQ70_08330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6067..6069,aa:Ser,seq:gga)
     gene            complement(6213..6404)
                     /locus_tag="PLQ70_08335"
     CDS             complement(6213..6404)
                     /locus_tag="PLQ70_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34659.1"
                     /translation="MKAETVFNVAIHLSDYELQRLINLIEDKLKLKPEKVLKKLFKRE
                     EEEKRMLRLIKTCFSKIKI"
BASE COUNT         2129 a         1109 c         1111 g         2132 t
ORIGIN      
        1 caaaaatcac gtgacaagcc agtccaattg ggatttgacc gtaatgttcg ctgaattgtt
       61 tcgttgtagc attgtaggtg tccaatttgg ctaaagcatt ggttccttcc ccatcataag
      121 acaaataaat ggcactattc gtgttgtcgt atccagttgg aacttcggct agaatagtcg
      181 ttctaggtct ggggtcacta tagaatttat ccacgtttgt ccagccaaag ttgccaaaag
      241 atacattata agtattttgt ttaaaatcaa cgtttccgtc tttttgccct ccagttcccg
      301 cagcagttcc aggtcttacc caagccaaat tttcaggatc ggtagtaatg tctcctgccc
      361 agaatagcat tcctgtatca atggttcctg ttaaatttgc gggtacttga agttgaatac
      421 cgccatttga cactaaatca actccgcctt gagaggcttt gatgaaaaat tctccacctg
      481 attttagtaa acttttttta ccatcagcca tcactcccat tgtaggtttg ttagtgatta
      541 gcatatttcc tttgtcgaaa agctcgataa aagtaatgtc gattgctcca gtaacaggac
      601 ttccgttttt agttagcgta ttcccgttta aagtgataat aactcctttt gcagatgtca
      661 aagttatggt tcctgttcca gcagttgctg tgaaattttg agtattactt ttgagtccgg
      721 cttcgtttat tcctttgaat gcagctgctg ttggaggaat tggatctaaa ttttcattgt
      781 tagtacagct tgcgaaaatc attgttgcta aagctaaaag tccgatgttt ttaatttgat
      841 ttttcataat ttctaatgtt ttaaaatggt tgtttatagt tatatcaatt cgggtttgat
      901 attgttaccc cttcaatctg aatttatttc cgattcttgt tttttttatc gagtaattaa
      961 tgcccagagt ttatggaaga aagtgattcc aaattttttt aaatttttca taatctaaaa
     1021 tttatatctt tttgtttgct tttacattat atcctggctt aacactactc tcttgtttca
     1081 aagtgctttt aggaggaaaa ggatttccat catcctgacc gtttggtctt gtagttcttt
     1141 cgttcgtttg attactttta gttcttgttt ttagaatatg aacaggttct gtagtttcgt
     1201 cagagataga agtcatttct ccagacatac tattttcttt aaaataagga ttggtaattg
     1261 caccagtgct tgccaaatcg cttttagctt ttacgatgtc ttgagttgat ttttctgttt
     1321 gtgcaaaacc aattgatgtt gctaaaacca ttagaagata atatttgtta atggtgtttt
     1381 tttctgattt gtgatgagtt gatttcataa tttctatttt taaaggatta taatttttga
     1441 tttataccgt tatatcaatt gggttttaaa tttgttaccc ctttaatctg aatttatttc
     1501 agattctttt ttacattagc agataaatat ttttttatgg cacaaagcac aattcatccc
     1561 gaccaaattt atatcgatgg acttgccaat aataattcgg cggtaattca atctatttac
     1621 aagaattttg tacctaaagt ggtttcgtat ataaggaaca attctggaga tcaagaccag
     1681 gcgcaagatg tggttcagga aattatgatt ttacttttta atcaggctaa agccaataaa
     1741 ttgcaactga cttgtccgtt tgacgcctac ttttttttat tgtgcaaaag aaaatggttg
     1801 aacgaactca aaaaatcttc gaataaaggg gtaacaatcg atgatgattt gccatctact
     1861 aatgaaccca ccgaacaaat ggttaccgaa accgaactat tcgacgaaaa acagcaactt
     1921 tttgacttaa tgtttcaaaa attgggcgac aaatgtcagg agttattgaa attgagtttc
     1981 accatcaaat cgatggagga agtagccgaa aaactcaatg taacttatgg ttatgtgaga
     2041 aagaaaaaat cgttgtgcgt tggtcaatta acccagtgga ttcaagaaac caatcacttt
     2101 caatctttaa aaattactta gccatgaacg aggaacgcta cataaaattc gatcaatatc
     2161 ttgccaacga aatgtctgcc gaagagaaaa cagttttcga aaaacaatta tccgaagatc
     2221 cagaattggc aacatcatta gaggttttca aagaattgca tctccatttg gaaaataaat
     2281 ttggaaaaaa agccgaattt aatgcctata aaaaaaatat agaatctatt tctaaaaaac
     2341 acttcaaaac caaaaaatcg aaagttattg atttgcgatc ttggcattat gcagtggcgg
     2401 cttctgtaac aattttattt ggattgtttc tttttcaaaa cataaatcca acttttgagg
     2461 attatgacaa tccagaaatg gccacttttg ttgaaagagg agatgtgaac gaaaatttaa
     2521 aattggcgca agatgctttt aatgcaaaag aatataaaaa agcaattcca atttttgaaa
     2581 cggttttaaa aaccaataaa tcacctgaaa ttcagtattt ttattccatt gcacttctgg
     2641 aagacaatca acttcaaaaa gccgaaatca atttttctga tttaagatat ggaaattcta
     2701 tttacaaaaa caaggcgact tggtatttgg cactttcaaa actcaaacag aaagatttta
     2761 gatcctgcaa ggatattctg ttgactattc ctgatgattt cgaagattac gaccaagttc
     2821 aggaattgtt gaacgaattg gattaggttt taattccacg gagattcaca aagagttttc
     2881 gcagagattc gcaaaaaatt gaattatttc tttgtgttac tttgtgttgc ctttgtgaat
     2941 ttttgcgtaa taattgtaag tttgcaactt aatatttgca tcaaataatg attattaccg
     3001 acacgcacac ccatttatat tccactgaat ttgaccaaga ccgcaacgaa atgattcagc
     3061 gtgccattga tgctggagtt acacgattct ttatccctgc tattgattct gcttatacgc
     3121 agtcgatgta tgatttggaa aaagcttatc ccgaaaatat ttttttgatg atgggcttac
     3181 atccaacgca cgtaaaagac aactatctcg atgaattaca acatgttgaa gaagaattat
     3241 ccaaaagaaa attctttgcc gtgggcgaaa ttggtatcga tttgtactgg gataaaaccc
     3301 atttaccgca acaacaagac gcttttagaa aacaaattca attagcaaaa caatacaaat
     3361 tgcccatcgt aattcattgt cgggaagctt tcgacgaaat tttcgaaata ttagaagcag
     3421 aaaaatcgac tgatttattc gggatttttc attgtttttc tggcacttat gagcaggcat
     3481 tacaagccat ttcgtacaat atgaaattgg gaattggcgg agttgtgact ttcaaaaacg
     3541 ggaaaataga ccaattttta agtcaaatcg atttgaaaca tattgttcta gaaaccgatt
     3601 ctccctattt ggcgcccgtt ccttttcgag gaaaacgcaa cgaaagtagt tatctaatta
     3661 atgttatcga taaactgtca atgatttaca atctgtcgaa agaggaaatc gctctgatta
     3721 caacccaaaa ttctaaagct atatttggga tttaatgtag atttcactac ttttcgataa
     3781 aatttttgtt catttgcctt ttaaaaaaca agttatatgt caaaatttga cgccattcgt
     3841 ccattttatg aaactgaagt aaacgatgcc attcaaggtg tgcttcatca ccctatgatg
     3901 aaagcattga tgaactttac atttccagat cttcctgacg aaatatggaa agaacagttg
     3961 ctacgaacgc attcaacgag agattttcaa tgtaacttta catacaaatc actcaaaaaa
     4021 attcttgaaa aaaaatcaga tggattgacc acttctggat ttgaaaagct tcaaaaaaac
     4081 acaccttatt tgtttatttc aaatcatcga gacatccttt tagatacttc attactaaat
     4141 gcttgtttgt tcgaaaataa attagtgatg actgcatcgg ccattggaga caatttggtg
     4201 caaaaatcat tcttgagaac actagcccga ttaaaccgaa acttcctagt tcaaagaggt
     4261 ttgtcgccac gcgaaatgtt gcaaagctca aaattattat ccgaatatat cagcatatta
     4321 ttgcaacacg aaaaccgttc tatttggata gcacaacgag aaggaagaac aaaagacggc
     4381 aatgacgcta caaatcctgg cgttttaaaa atgctggcta tggcatctga cgaagaaaat
     4441 ttgatggatt atttcaagaa aattcaaata gttcccgttt ctatttcata cgaatacgac
     4501 ccaaccgact tcttgaaaat gccacaactt ttggcagaag ccaaacaaga aatttacatc
     4561 aaagaaaaaa atgaggattt catcaacctt atgagtgggg ttatgggtca aaaaaaacgc
     4621 attcacattc acgttggaga tgttctagat aaagaattgg atattataaa agagctacac
     4681 gaaaactcaa acaaacaaat tcaagctttg gcacaaataa tagacgattc tattttaagc
     4741 aattataaac tttggcctac caattatatt gcttacgata ttttgaataa aaccacaacc
     4801 tatcagcatt tgtacaccga aaacgaaaaa tcgctttttg aacgcagatt agaaatgagg
     4861 atagatcata gcaatccatt ggcgttgcaa ggtttcttgt ctatgtatgc gaatccggtt
     4921 gtgaataaat taaaatacga aaatgctatc taaagccaaa atcctcttga tttataccgg
     4981 cggaaccatt ggtatgatga aagattttga aacaggagca ctcaaggctt ttaattttag
     5041 aaaattgctg caaaaaatcc ctgagttaaa acaattggat tgcgacatcg agacagtttc
     5101 tttcgaaaaa ccaatcgatt cttctaatat gaatcccgag aattggacag aaattgctca
     5161 aatcatagaa aacaattttg acaattttga tggatttgta gtgctccacg gttcggacac
     5221 gatgtcttat tctgcatcgg cattgagttt tatgctcgaa aatttatcaa aacccgtgat
     5281 ttttacaggc tcgcaattgc ccataggcga tttaagaacc gatgccaaag aaaatctcat
     5341 aacagccatt caaattgcgt cgttaaaaga aaacgacaaa cccctaatta gcgaggtatg
     5401 cctttatttt gaatacaaat tatatcgagg caatagaacg accaagataa atgccgaaaa
     5461 tttcaatgcg tttatttctc caaattatcc cactttgata gaatcgggag tgaatttgaa
     5521 gacttttccc gatttgtttt tgccacagac caaggcaaaa aaacttaaaa ttcataaaaa
     5581 attagacact aacgtagcca ttataaaaat gtttcctggc attagtgaaa ccgttttatc
     5641 ggcaattttt gatattccgc aactaaaagg aatcgtatta gaaacctatg gtgcaggaaa
     5701 tgctccaacc gaagattggt ttttatcctt actaaaaaaa gccattgcca acggattgta
     5761 tatcgttaat gtgacccaat gctccatcgg aagtgtgaac atgggaaaat atgaaacgag
     5821 cactgccttg aaggaaattg gagtaatttc tggcaaagac atcactaccg aagctgccat
     5881 taccaaattg atgtacttgc tgggacaaaa tgtttcgaaa gaaaatttca aaaccctttt
     5941 cgaaacttct ttaagaggag aaatttcata aaaaccgttt tttaattttc tttaatcatt
     6001 ttttttagtg ttatttgcac cctcaaacaa ctagagaggt gtccgagtgg ttgaaggagc
     6061 tagcctggaa agctagtata tgggtaactg tatcgagggt tcgaatccct tcctctctgc
     6121 tgaaaagctt ttaaaccctt attttataag ggttttgttt tttaggggca taattagggg
     6181 cataaaatat ttttaatttg tgtttaaaaa aattatattt tgatttttga aaaacacgtt
     6241 ttaattaaac gaagcatcct tttttcttct tcttcccttt tgaaaagttt cttcaatact
     6301 ttttcaggtt taagttttaa tttatcctcg attaaattaa ttaatctttg aagttcataa
     6361 tccgaaaggt gaatggcaac attaaatacg gtttctgctt tcatatttcc gtctgtatca
     6421 aatataaatg tataatttaa tacaatggga tgaattattc tcaactaata attcgaaaat
     6481 g
//
LOCUS       DAOSNN010000152         6452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12094_length_6452_cov_5.064874, whole genome shotgun sequence.
ACCESSION   DAOSNN010000152 DAOSNN010000000
VERSION     DAOSNN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12094_length_6452_cov_5.064874"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            441..695
                     /locus_tag="PLQ70_08340"
     CDS             441..695
                     /locus_tag="PLQ70_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34660.1"
                     /translation="MTVDILSSIKGAQPSESVNKLFDVIKNASSNNNPSNNVNYNCVS
                     VSDLREDVVVESSTMEKQLILENFPNKKNGFLVVAKVIEG"
     gene            697..2094
                     /locus_tag="PLQ70_08345"
     CDS             697..2094
                     /locus_tag="PLQ70_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQE34661.1"
                     /translation="MESQIKKIHQQLVSKQISCTALVQEKLDLLKQNTYNSVNSVLDT
                     LALELASKVDAKIANGEPIGLLEGIPFGIKDVYMVQGTYTTASSDLLKNYKSPYTATA
                     IQKLLDAGAIPLVKENCDSFGHGSSSENTIFGAVKNAINPELVAGGSSGGSAVNVAKD
                     YTVFSIGGDTGGSIRQPAGYNHIYGFKPTYGRISRYGLMAYASSTDCVGPLAKSIEDI
                     RIVLNVMSGKDPKDQTSVTSTEISEEAIATSTVETIGYFKNFIESDAIDPQIKADFLA
                     SIEKIKAKGIEVKELDFFKSDILVSTYYTLAMAETASNLSRLDGTNYGNRIEGENLID
                     TYAVTRSENFSEETKRRIVGGNQVLSQGFSEGIYLKGLALRDQISQNFSKDFEEVNIV
                     LSPVTPSTPPKIGDSLKDPLAMYLSDAYTVGFSLGQLPTLTVPQGTSTGLQITAAKNN
                     DELVLQFANFLKDTI"
     gene            2094..5426
                     /gene="gatB/aspS"
                     /locus_tag="PLQ70_08350"
     CDS             2094..5426
                     /gene="gatB/aspS"
                     /locus_tag="PLQ70_08350"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035670674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional amidotransferase subunit
                     GatB/aspartate--tRNA ligase AspS"
                     /protein_id="HQE34662.1"
                     /translation="MELEQLTAAIKAHDLELVIGLETHVRLNTKTKLFCSCPNQEIET
                     PNQNICSVCTGQMGVLPAVNKEAVTKAIYFGKAVDSSFSNEVISWDRKHYEYPDNPKN
                     IQITQFHNPIIPDGHVSCYRNDGTQFTVNLTQVHIEEDAAKLMHEKKISLVDFNKAGV
                     PLIEIVTEPCIRNIEDASTYAQYIQRIVQNLGISEANLEKGEFKSDVSVSLRKKHSYD
                     LNPRTEIKNLNSFKFMVEALKEEVEKQFNYFIENKEFRPDQTTVLWDADLKQTKTMRK
                     KEFEADYRFISEPDLPFVNIKKEIEAIKVDTDALPFAVESILIHGGVLPQDAKFFTAD
                     ALRSGTFVAINKVINDPSFVAKTLANNLKVDDYAEIHSIDHLIEIFQLFKAEKITAVL
                     VQNAIIGYLKDRTFDYNSYFAENTISEDKIQEVIAKVLSENEAVANDIKAGDQGKAGI
                     LVGKILGIIGKGANGKVIRQQILDKLGAAAVLEKSEASEKVSKEAVAENKEIQEEVLP
                     EIPIIIKDTYRTHKISQLSEENIQDEVLLSGWVASVRDHGELMFIDLRDSSYEIFQVR
                     ISRESFPNIDELVKLKPESVISVKGIVVGRNEDDFNAGLRTGKIELETSVLEILNLSK
                     TLPFEIKRAAKTNETTRFQYKFLDHRNEEVRRAIVNRHKVIKLLRDILDEEEFLEIET
                     PILSAGTDEGAREFIVPTRKQAGFFYTLPQAPQQFKQMLMVSGYEKYFQIARCFRDED
                     SRGDRQPEFTQLDIEMAYASMQQIIDLNTKMFNEVVKKIYGNKWILRPFEVITYKDAM
                     DFYGCDRPDLRYGLKMQDITDIVKDTTFQVFSKPIEEGGIVKCIKVSAQEQGNKRMSK
                     GQIETLTAVAQQHGLGGLAYIIVNEDELQSPIIKFLGEEIAAGIIKATNAQVGDIVFF
                     SAADYATANKALDAVRQEMGRMLHLINPKELRPAWVVDFPMFERTDEGRWTFTHNPFS
                     MPAIYDLQKHMEGDDEKIGTIIAQQYDIILNGYEIGGGSVRAHKSEILEATYRNMGYN
                     KEEMMKSVGTMYKAFQYGAPPHGGIAWGIDRLMMILEKKASIREVMAFPKTGTSEDLL
                     FGAPSLLSDKKVEEMNVKIMRK"
     gene            complement(5550..6008)
                     /locus_tag="PLQ70_08355"
     CDS             complement(5550..6008)
                     /locus_tag="PLQ70_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQE34663.1"
                     /translation="MNDNFITEFFGIGIQNGKTPENLGVLWRSAQNLGASYIFTIGNR
                     YAKQACDTHNAVKSMPYFHYETFEDFYKNLPKGARLVGVELDENAEDLETFEHPRRCV
                     YLLGAEDNGLSKKAIEKCHYLVKFKSKKSLNVSVAGTIVLYDRGLNKPRS"
BASE COUNT         2202 a          977 c         1281 g         1992 t
ORIGIN      
        1 actcgctcaa agacatagtg accggagttg caatattgta ggtttcataa ttttcatctc
       61 cattctcaca agacataaaa gtcatcgaaa agaaagttat tattagcaaa aaatataaag
      121 tatttttcat ttgtgtatta ttttgtaagt ttatatcgtg gtgctaattt attcattttt
      181 aatgaaataa tggttgtgtt tggattgttt ttttttaata attttaaaat ttgatagttt
      241 gtgtttttgt tagtctattt atatattttt aatagttttc gtccaaagaa tttaaatcta
      301 acataatgtt aaattaatat cttctttcac aaaaaactac cgaaatcatt aaaataaaac
      361 atcattctct aataaaactt tcgtaatttc gccaccttaa aagtttgact ttaaaaccaa
      421 atacaattat gagcaaaata atgacagtcg acatattgtc gagtattaaa ggagcacagc
      481 catccgaaag cgtgaacaag ttatttgatg taatcaaaaa tgcatcatca aataataatc
      541 cttctaataa tgtcaattat aattgcgtgt cagtaagtga tttaagagaa gatgttgtag
      601 tagaaagttc gactatggag aaacaattaa ttttggaaaa tttcccgaac aagaaaaatg
      661 gttttttagt ggttgctaaa gttatagaag gataaaatgg aatctcaaat aaaaaagata
      721 caccagcaat tggtgtcaaa acaaataagt tgtacggctc ttgtacagga aaaattagac
      781 ttacttaaac aaaataccta caattctgta aattctgttt tagatacttt ggctttagaa
      841 ttggcttcaa aagtagatgc taaaattgca aacggagaac caatcggtct tctggaaggt
      901 attccctttg gaatcaagga tgtttatatg gtgcaaggaa cttacactac agcaagttct
      961 gatttgttga aaaattataa atcaccttac acagctactg ccattcaaaa attattggat
     1021 gccggagcaa ttccgttggt aaaagaaaat tgtgatagtt ttggtcacgg atcttcaagt
     1081 gaaaacacca tttttggagc ggtgaaaaat gcaattaatc cagaattagt ggcaggaggt
     1141 tcaagcggtg gttctgcggt taatgttgcc aaagattata ctgttttttc tattggtgga
     1201 gatacaggag gttctattcg tcagcctgca gggtataatc atatttacgg tttcaaacca
     1261 acttacggaa gaatttcaag atacggattg atggcgtatg catcttctac ggattgcgtt
     1321 ggtccattgg cgaaatcaat tgaagatatt agaattgtgc tgaatgtaat gagtgggaaa
     1381 gatccaaaag atcaaacttc agtcacttca actgaaatca gtgaagaggc gattgcaact
     1441 tcaacagttg aaacaattgg ttatttcaaa aactttatcg aaagcgatgc tatcgatcct
     1501 caaataaaag ctgatttctt ggcaagtatc gaaaagataa aagccaaagg aattgaagta
     1561 aaagaattag attttttcaa atctgatatt ttggtttcta cttattatac attggcaatg
     1621 gctgaaacgg cttctaattt atctcgtttg gacggaacaa attatggtaa ccgtattgaa
     1681 ggcgaaaatt taattgatac ttatgccgtt acccgttctg aaaatttttc ggaagaaact
     1741 aaacgcagaa ttgtaggtgg aaatcaagtg ttgtctcaag gtttttcaga aggaatttat
     1801 ttaaaaggat tggctttgag agaccagatt tctcaaaatt tcagcaaaga ttttgaagaa
     1861 gtaaatatcg ttttatcgcc agttacacca agtacgcctc ctaaaatcgg agacagttta
     1921 aaagatccgt tggctatgta tttgtccgat gcctacacgg ttggttttag cttgggtcaa
     1981 ttgccaacat tgactgtgcc acaaggaacg agtacaggac tgcaaattac ggcagcaaaa
     2041 aataatgatg aattagtgtt gcaatttgca aacttcttaa aagatacaat ataatggaat
     2101 tggagcaatt aacagcggct ataaaagccc acgatttaga attggtaatt ggactggaaa
     2161 cacacgttcg attgaatacc aaaaccaagt tgttttgttc ttgtccaaat caagaaatag
     2221 aaacacctaa ccaaaatata tgttcggttt gtactggaca aatgggcgtt ttgcctgccg
     2281 taaacaaaga agcggttaca aaagcgattt actttggaaa agctgtggat tcgtcattta
     2341 gcaatgaagt gatatcttgg gatagaaagc attacgaata cccggataac ccaaaaaaca
     2401 ttcagattac gcaatttcac aacccaataa tccctgacgg acacgtttct tgttaccgaa
     2461 atgacggtac tcaatttacc gtaaatttaa ctcaggtgca tattgaggaa gatgctgcca
     2521 aattgatgca cgaaaagaaa atttcgttag ttgattttaa caaagcaggt gttccgttga
     2581 tcgaaattgt tacggaacct tgtatccgta atattgaaga tgcttcaact tatgctcagt
     2641 acattcaacg tattgttcaa aacttaggaa tttctgaagc aaaccttgaa aaaggagaat
     2701 ttaagtcgga tgtttctgtg tctttgcgta aaaagcacag ttacgatttg aatccaagaa
     2761 cggaaatcaa aaacttgaac tcgtttaagt ttatggtcga agccttgaaa gaagaagtag
     2821 aaaaacagtt caattatttt attgaaaata aagaattcag acctgaccaa acgacggtgc
     2881 tttgggatgc cgatttaaag cagaccaaga caatgcgtaa aaaagaattt gaagcagatt
     2941 accgtttcat ttctgagcca gatttacctt ttgtaaacat caagaaggaa atcgaagcga
     3001 tcaaagtgga tacggatgct ttgccttttg cggtcgaatc tattttaatt cacggaggtg
     3061 tattgccaca agatgctaag ttttttacag cagatgcttt gcgttctgga acttttgtag
     3121 cgataaataa ggtgattaac gatccttcgt ttgtagctaa aactttggcc aataatttga
     3181 aagttgatga ttatgccgaa atccacagta ttgatcattt aattgaaatt ttccaattat
     3241 tcaaagccga gaaaatcact gctgttttag ttcaaaatgc gattattggt tatctaaaag
     3301 atagaacttt tgactataat agttactttg ctgagaatac catttcagaa gataaaattc
     3361 aggaagtaat tgcaaaagta ctttccgaaa atgaggctgt tgccaatgat atcaaagcgg
     3421 gagaccaagg gaaagcaggt attttagtcg gcaagatttt aggtattatc ggaaaaggtg
     3481 ctaacggaaa agtaattcgt cagcaaattt tagataaatt gggtgctgct gctgttttag
     3541 aaaagtcaga agcatcagag aaagtttcca aagaagctgt ggcagaaaat aaagaaattc
     3601 aagaagaagt gcttcctgaa attccaatta ttataaaaga tacttataga acgcacaaaa
     3661 tttcacagtt atcagaagaa aacattcaag atgaagtgct attgtcgggt tgggtagcta
     3721 gtgttcgtga tcacggtgag ttgatgttta tcgatttgcg tgattccagt tatgagattt
     3781 tccaagtccg tatcagcagg gaatcatttc ctaatattga tgagttagtg aaattgaaac
     3841 cggaatcggt tatttcggta aaaggaattg tagtaggtcg taatgaagat gattttaatg
     3901 caggattgcg taccggtaaa attgaattgg aaacttcagt tttagaaatt ttaaacttat
     3961 ccaaaacctt gcctttcgaa atcaagagag cggctaagac aaatgaaacg actcgttttc
     4021 aatacaaatt tttagatcac agaaacgaag aggtaagacg agccatcgta aaccgtcata
     4081 aagttattaa attattgcgt gacattttag atgaagaaga attcttagaa attgaaacgc
     4141 cgattttaag cgcaggaacg gatgaaggtg cacgtgaatt tattgttcct acacgtaaac
     4201 aagccggttt cttttataca ttgccacaag cgccacagca gtttaagcaa atgttgatgg
     4261 tgagtggata tgaaaaatat ttccaaattg cgcgttgttt tagagatgag gattcccgtg
     4321 gagaccgtca gccggaattt acgcaattgg atattgaaat ggcttacgcc agtatgcagc
     4381 aaattattga tttaaacact aaaatgttta atgaagtagt gaagaaaata tacggcaaca
     4441 aatggatttt gcgtccgttt gaagtcatta catataaaga tgcaatggat ttctatggtt
     4501 gcgacagacc tgatttgcgt tatggtttga aaatgcaaga catcactgat atcgtaaaag
     4561 ataccacttt ccaagtgttc agtaagccta ttgaagaagg tggaattgta aaatgtatca
     4621 aagtttcggc tcaagagcaa ggaaacaagc gtatgtctaa agggcagatt gaaacgctta
     4681 cagcagttgc gcaacaacac ggtttgggcg gattggctta tattattgta aacgaagacg
     4741 aattgcaatc accaattatt aaattcttgg gtgaagaaat tgctgcagga atcataaaag
     4801 cgaccaatgc acaagtgggt gatattgtat tcttttccgc ggcagattat gcaacggcta
     4861 acaaagcttt ggatgcggtt cgtcaggaaa tgggtagaat gttacacttg attaatccga
     4921 aggaattacg tccggcttgg gtggtagatt ttccaatgtt cgaaagaact gatgaaggaa
     4981 gatggacatt tacacacaat ccgttctcta tgccggctat ttatgatttg caaaagcata
     5041 tggaagggga tgatgaaaaa atcggaacca tcatcgctca gcaatacgac attatcttaa
     5101 atggttacga aattggtgga ggatcggttc gtgcccataa atcggaaatt ctggaagcga
     5161 cttatagaaa tatgggttac aataaagaag aaatgatgaa aagtgtaggg acaatgtata
     5221 aagctttcca atacggcgca ccgccacacg gaggaatcgc ttggggaatt gaccgtttga
     5281 tgatgatttt ggagaaaaaa gcttctatta gggaagtaat ggctttccca aaaacaggaa
     5341 cttcggaaga tttattattc ggcgcaccat ctcttttatc tgacaaaaag gtagaagaaa
     5401 tgaatgtgaa gattatgagg aaatagattt taaaatctaa aaatatttaa aaaagcggat
     5461 ttgtatcgaa agatagaact ctgctttttt tatggaaaaa tgatatgtaa acttatttta
     5521 tgatgaagta aaataaaatg cagtttgtct tacgatctcg gtttgttaag gcctctatca
     5581 taaagaacaa tcgttccggc cactgacaca ttcaagctct ttttggattt gaattttact
     5641 aaataatggc atttttcaat cgctttttta gaaaggccat tgtcttcggc acccaataaa
     5701 tagacacatc gtcttgggtg ttcaaatgtt tccaaatctt ctgcattttc atctaattcc
     5761 acaccaacca atcgggcacc tttgggcaag tttttataga agtcttcaaa ggtttcgtaa
     5821 tgaaaataag gcatcgattt taccgcatta tgcgtatcgc aagcttgttt ggcatagcgg
     5881 tttccaatgg taaaaatata gctggcaccc agattttgtg cagatcgcca taagacaccc
     5941 aagttttcgg gtgtttttcc attttgtatt ccgatgccga aaaattctgt tataaaatta
     6001 tcattcatac tgcaaaaata cgaactgtta gtagtatttt aaattttaaa ttcatgggtt
     6061 ttaaataaag tttgaaactt tgcattttag tgtattttgc tttcaggcta taaaaaagtt
     6121 ttttccaaat tttaaaaaat aatctgttga aatttgtgga atttgtggtt gatttttttt
     6181 agttctatta aaattgacta ttacattatt atcgaaacta ccattaccat tgaaattgat
     6241 tttcctcatc gaaattgaca cacttttgtt aataacttaa caacttaaaa tgggatttta
     6301 atgattaatt cctatacggt ttcttatatt tgcgtgtaat accaaaaaca catttttaga
     6361 gtaaatttat atgagcgaag aaatcaagaa ggagaataat tattcagcag atagtattca
     6421 ggcattagaa ggaatggagc acgtaagaat gc
//
LOCUS       DAOSNN010000153         6430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12162_length_6430_cov_5.241725, whole genome shotgun sequence.
ACCESSION   DAOSNN010000153 DAOSNN010000000
VERSION     DAOSNN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12162_length_6430_cov_5.241725"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLQ70_08360"
     CDS             complement(<1..485)
                     /locus_tag="PLQ70_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34664.1"
                     /translation="MKPNSPSLFLYFAATTLVVLFKIFEWDSYVLYAKSIIIPLLFIY
                     YFITNNYKIGRIKALIFLFCFIGDLFNLLNFTFSALGALLSFLVVYLLLLKLTYDDFR
                     SLKFKNKDRFPVLITFLFVATISTSVLSLQFENLALDFSLYVIYAIILSLLVFVSISN
                     Y"
     gene            complement(472..2478)
                     /gene="ligA"
                     /locus_tag="PLQ70_08365"
     CDS             complement(472..2478)
                     /gene="ligA"
                     /locus_tag="PLQ70_08365"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQE34665.1"
                     /translation="MDIQNTIQKLREELNQHNHNYYVLDTPTISDFEFDQKLKQLQGL
                     EQLHPEYFDENSPTQRVGGAVTKNFETVKHDHRMYSLDNSYSKEELVDWEKRVQKVLG
                     DVPLEYTCELKYDGASISITYENGKLKRAVTRGDGVQGDDVTNNIKTIKSIPLKLKGN
                     FPDKFDVRGEIILPFAGFEKMNQELIEIGETPYSNPRNTASGSLKLQDSAEVAKRPLD
                     CLLYFLIGNKLPFNTQFDGLETARNWGFKVPKESKLANSLEEVFQFIDYWDVHRHDLA
                     YETDGVVIKVNDFQQQEELGFTAKSPRWAIAYKFKSEQVFTRLNSISYQVGRTGAITP
                     VANLEPVQLAGTIVKRASLHNADQIEKLDIRIGDTVFVEKGGEIIPKIIAVDLSKRPE
                     LSEPTKYITHCPECQTELVRGDGEANHYCQNFYGCPPQIIGRIQHYISRKAMDIEGLG
                     GETVALLYNNGLVQNYADLYQLTVGDILPLERMAQKSAENLVNGVENSKNIPFERVLY
                     ALGIRFVGETVAKKLAKHYKNIQALTQASLMDLVLVDEIGERIAQSVIDFFENQENSL
                     IIERLKNYGVQFETVEIINPNATNKLSGKTFVVSGVFEQFSRDDLKKAIEDNGGKVGS
                     SISAKTDFVVAGANMGPAKLEKAVKLNIPIISEDDFKTMLNEAE"
     gene            complement(2549..3022)
                     /locus_tag="PLQ70_08370"
     CDS             complement(2549..3022)
                     /locus_tag="PLQ70_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6495 family protein"
                     /protein_id="HQE34666.1"
                     /translation="MKYSRLTKEQFEELHQEFSTFLATQTIDKVEWDQLKIEKPEVAE
                     QELDVFSDLVWEGVLSRAEYLEHFSKNHIFLFQCFDNYIQSIVLKSLVPETDFLTKDG
                     LQWLSDNMFTETIEIKTGKKVFTDERNSSIFQLIQQGAILSEGQLFKQIISIIES"
     gene            complement(3151..3594)
                     /gene="rplI"
                     /locus_tag="PLQ70_08375"
     CDS             complement(3151..3594)
                     /gene="rplI"
                     /locus_tag="PLQ70_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQE34667.1"
                     /translation="MELILKKDVQNLGFKDDVVNVKNGYGRNFLIPQGFAQLATPSAK
                     KVLAENLKQRAHKEAKVVADAKALAETLKALEIKIFAKAGGEKLFGSITNIDIAEALE
                     KAGQSIERKFITSGIVKRTGKYAASIRLHRDVVVELAYEIIAEKA"
     gene            complement(3660..3956)
                     /gene="rpsR"
                     /locus_tag="PLQ70_08380"
     CDS             complement(3660..3956)
                     /gene="rpsR"
                     /locus_tag="PLQ70_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQE34668.1"
                     /translation="MATLQQSASGKKDGDIRYLTPLNIETNKTKKYCRFKKSGIKYID
                     YKDADFLLKFVNEQGKILPRRLTGTSLKYQRKVSVAVKRARHLALMPYVADLLK"
     gene            complement(3962..4303)
                     /gene="rpsF"
                     /locus_tag="PLQ70_08385"
     CDS             complement(3962..4303)
                     /gene="rpsF"
                     /locus_tag="PLQ70_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQE34669.1"
                     /translation="MNHYETVFILNPVLSEVQVKETVSKFEDFLTSRGAEMVSKEDWG
                     LKKMAYEIQNKKSGFYHLFEFKVAGEVLIAFETEFRRDERVMRFLTVSLDKHAISWAE
                     RRRAKLKSQKA"
     gene            4470..5165
                     /locus_tag="PLQ70_08390"
     CDS             4470..5165
                     /locus_tag="PLQ70_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQE34670.1"
                     /translation="MKLNCVVVDDSSIQRMIITKLVNNHPNLHLIGDFSNAIEAKGCM
                     TIHNVDLIFLDIEMPVISGFDFLDGLKTKPQIIFITSKAEYAMKAFDYDATDYLQKPI
                     AVDRFNASVKRAIDMHLLKSETHDEDGEHIFIKSNLKKLKIFTAKIKWIEAFGDYVKV
                     VTEEDSNLVLSTMKSFEKDLPKDKFVRVHKSYIINIDKVERFNSRFAEIGVTKIPLSR
                     HKKEDLVKALAIN"
     gene            complement(5162..>6430)
                     /gene="priA"
                     /locus_tag="PLQ70_08395"
     CDS             complement(5162..>6430)
                     /gene="priA"
                     /locus_tag="PLQ70_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQE34671.1"
                     /translation="NLGLVIVDEEHEQTFKQVDPSPRYHARDASIVLANSHKAKVLLG
                     SATPSIETYFNSQSGKYGLVEISKRYGNVKMPEIELVNLKDKYFRKQMSGHFSDILIE
                     EITKTLSLGEQVILFQNRRGFSPIIECLTCGHVPQCPQCDVSLTYHKHKNQLRCHYCG
                     YSMAKPTNCHSCSSVHLTTKGFGTEQIQQELVELFSNTKIGRMDQDTTRGKFGFEKII
                     DSFKNREIDILVGTQMLAKGLDFDNVSLVGIMNADNMLYHPDFRAFERSYQMMTQVSG
                     RSGRSEKQGKVIIQTYNPDHNIIQQVVHNNYIGMYKEQLYDRQIYHYPPYYKLIKLTL
                     KHRDFDKLKEGSMWLYQVMKQNFTIPILGPEEPPISRIRNEYIRTIMVKIPTNQSLQG
                     TKKTIQKILNSFDVVPQYRAIKVSVNVDFY"
BASE COUNT         2110 a         1140 c          949 g         2231 t
ORIGIN      
        1 atataatttg aaatcgaaac aaaaactaaa agacttaaaa taatggcata aattacatat
       61 aacgaaaagt ctaaagccaa attttcaaat tgcagactta aaaccgaagt actgatagtt
      121 gcaacgaaca agaaggtgat taaaactgga aaacgatcct tgtttttaaa ttttaaactt
      181 ctaaaatcat cataagtaag ttttagtagc aataaataaa ctactaaaaa ggacagtaaa
      241 gctcctaaag ccgaaaaagt aaaattcaac aaattgaata aatctccaat aaaacaaaac
      301 aaaaaaatca atgcttttat ccttcctatt ttataattat ttgtaataaa gtaataaata
      361 aatagtaatg gtatgattat cgattttgca taaagaacat aggaatccca ttcaaatatc
      421 ttaaataaca caaccaaagt agtagctgca aaatataaaa ataaagaagg gctattcggc
      481 ttcattcaac attgttttaa aatcatcttc cgaaataatc gggatattca acttgactgc
      541 tttttccaat ttagctggtc ccatattcgc tccagcaaca acaaaatcag tctttgccga
      601 aatggaactt cctacttttc cgccattgtc ttcgatagct ttctttaaat catctctcga
      661 aaattgttca aaaacacccg aaactacaaa tgtttttcca gagagtttat ttgtcgcatt
      721 tggatttata atttcgacag tttcaaattg aactccgtaa ttttttaatc gttcaataat
      781 aaggctattt tcttgatttt caaagaaatc aatcacactt tgtgctattc gctctccaat
      841 ttcgtcaacc aaaaccaaat ccatcaagct ggcttgagtc aatgcttgaa tgtttttata
      901 atgtttggct aattttttag ctacagtttc tccaacaaat cgaatgccta gtgcatacaa
      961 aactcgttcg aatggaatat tcttagaatt ttcgactcca ttgactaaat tttctgccga
     1021 tttttgagcc attctttcca aaggaagaat atctcctaca gtcaattgat acaaatcggc
     1081 ataattttgc accaaaccat tattatagag caaagcaacg gtttctccgc caagaccttc
     1141 aatgtccatc gcttttctag aaatataatg ttggattctt ccaataattt gtggtggaca
     1201 accataaaaa ttctggcaat aatgatttgc ttctccatct cctcgaacta attcggtttg
     1261 gcattctgga caatgtgtaa tgtattttgt aggttctgaa agttcgggtc ttttgcttaa
     1321 atcaactgca ataatttttg gaataatctc gccccctttt tctacaaaaa cagtatctcc
     1381 aatgcgaata tccaactttt cgatttgatc ggcattgtgc aaagaagctc ttttcacgat
     1441 agttccagcc aattgtacag gctctaaatt agctactgga gtaatcgctc ccgttcttcc
     1501 tacttgataa gaaatggaat ttaatcgggt gaaaacttgt tcggatttga atttgtaagc
     1561 aattgcccaa cgtggagatt tggccgtaaa acccaattct tcctgttgtt gaaaatcatt
     1621 tacttttatt acaacaccgt ctgtttcata agcaagatcg tgtctgtgca catcccaata
     1681 gtctataaat tgaaaaactt cctctaaact attagctaat ttggattctt ttggcacttt
     1741 gaacccccaa tttcgggcag tttctaaacc atcaaactga gtattaaaag gtaacttatt
     1801 tccaattaga aaatacaaca aacaatctaa tggtcgttta gctacttcag cgctatcttg
     1861 caatttcaaa cttcccgaag ccgtatttct tgggttagaa taaggtgttt ctcctatttc
     1921 aatcaattct tgattcattt tttcgaatcc agcaaaaggc aaaatgattt cgccacgaac
     1981 gtcaaattta tctggaaaat taccttttaa ttttaaggga atagatttta tagttttgat
     2041 attatttgta acatcatcgc cttgaactcc atctccacga gtgacggcgc gttttagttt
     2101 tccattttca taagtgatgc tgattgatgc accgtcgtat ttcaattcac aagtatattc
     2161 taacggaaca tctcccaaca ctttttgaac acgcttttcc caatctacta attcttcttt
     2221 agaataagaa ttatccaaag aatacattct atggtcgtgt tttacggttt caaagttttt
     2281 ggtgactgct ccgcccacac gttgagttgg agaattttca tcaaaatatt cgggatgtag
     2341 ctgctctaac ccttgaagtt gtttgagttt ttggtcaaat tcaaaatcag aaatagtagg
     2401 tgtatccaac acataataat tatggttgtg ctggtttaat tcttctctta acttttgaat
     2461 tgtattttga atatccataa aaaaattggc tattttagtt cttaaaataa aacactaaaa
     2521 taaccaatta aatagaaaaa cgatacaatt acgattcaat aatcgaaatt atttgtttga
     2581 ataattgtcc ttcactcaaa atcgctcctt gttgtatcaa ttgaaaaata gaactattgc
     2641 gttcatcggt aaatactttt ttacctgttt tgatttcgat ggtttcggta aacatattgt
     2701 cgcttaacca ttgcaaaccg tctttcgtta aaaaatcggt ttcaggaacc aacgatttca
     2761 agacaattga ttgaatataa ttatcaaaac attgaaaaag gaaaatatga tttttagaaa
     2821 aatgttctaa atattcggct ctactcaaaa caccttccca taccaaatct gaaaaaacat
     2881 ctaattcttg ttcagcaact tctggttttt caatttttaa ttgatcccat tcgactttat
     2941 cgatagtttg agtggcaaga aaagtactaa attcttgatg taattcttcg aattgttctt
     3001 tggttaatct gctatacttc atttatactg aacttgtttc agtatcttct ataagatacg
     3061 gtaattaaaa aaatgaaaat ctgaaataaa ttcagattaa acaaaaaaaa tcccgacttt
     3121 catcgggatt taattttatg aatttagaaa ttatgctttt tcagcaatga tttcataagc
     3181 taactcaact actacatctc tgtgtaatct gatgcttgca gcatatttac ctgtacgttt
     3241 tacgatacca gaagtaataa attttctttc gattgattga ccagcttttt ccaaagcttc
     3301 agcaatatcg atgtttgtga tagatccaaa aagtttttct ccacccgctt ttgcgaaaat
     3361 tttaatctct aatgctttca aagtttcagc caatgctttt gcatctgcaa ctactttagc
     3421 ttctttgtgt gctctttgtt ttaggttctc agccaatact ttctttgcag aaggagtagc
     3481 taattgagca aatccttgtg ggattaaaaa attacgaccg tatccgtttt ttacgtttac
     3541 tacatcgtct ttaaaaccta aattttgaac gtcttttttt aatataagtt ccatgttgtt
     3601 gtccttatta agaagttagg tttcatattt cagaaaacca acaactgtta attttttaat
     3661 tattttaata aatcggccac gtatggcatt aatgctaagt gacgagctct ttttacagct
     3721 acagacactt ttctttggta tttcaaagaa gtacctgtca aacgacgagg aagaattttt
     3781 ccttgttcgt taacaaattt caataagaaa tcagcatctt tataatcaat atatttgata
     3841 cctgattttt tgaaacgaca atacttttta gttttgttag tttctatgtt taaaggagta
     3901 agatatctga tatctccgtc tttttttcct gaagcagatt gttgtaatgt tgccatgata
     3961 attaagcttt ttgagattta agttttgctc ttcttctttc agcccaagag atagcatgtt
     4021 tgtcaagact tacagttaag aaacgcataa ctctttcgtc acgtctaaat tcagtttcaa
     4081 aagcgataag aacctcacca gcaactttga attcgaataa atggtaaaaa ccactttttt
     4141 tgttttggat ttcgtaagcc atctttttaa gaccccagtc ttctttagac accatttcag
     4201 ctcctctact agtaagaaaa tcttcaaatt tgcttactgt ttcctttacc tgaacttcag
     4261 ataaaacggg atttaaaatg aaaacagttt cataatgatt cataaatatg tatgtatttg
     4321 ttaaattggc tgcaaaagta atcttttatt tctaatatcc aaacaaaatt aaactttaat
     4381 cgtcataata caaaaaaaac cgataaagat taggttaatt gaattccatg tattactttt
     4441 actatcccga atctaaaata ataagaatta tgaaactaaa ttgtgttgta gtagatgata
     4501 gttccattca gagaatgatt atcacaaagt tagtaaataa tcaccccaat ttacatttaa
     4561 ttggtgattt ttctaacgct attgaagcaa aaggctgtat gaccatacat aatgtggatc
     4621 tgatcttttt agacattgaa atgcccgtaa ttagtggttt tgattttttg gatggtttaa
     4681 aaactaaacc ccaaattatt tttatcacct caaaggcaga atatgctatg aaggcttttg
     4741 attatgatgc aactgactat ctgcaaaaac ctattgcagt cgatcgtttt aatgcgtctg
     4801 tcaaaagagc gattgatatg catttgctaa aatcagaaac ccatgatgaa gatggtgaac
     4861 acatctttat taaaagcaat ctaaaaaaat taaaaatctt tactgcaaag attaaatgga
     4921 ttgaagcttt tggtgattat gttaaagttg taaccgaaga agacagtaat ttggtacttt
     4981 ctaccatgaa atctttcgaa aaagatttac caaaagacaa atttgttaga gtgcacaaat
     5041 cttacattat caatattgat aaagtggaac gcttcaacag caggtttgcc gaaattggag
     5101 ttacaaaaat tcctttaagc agacataaaa aagaagattt agtgaaagct ttagctatta
     5161 actaataaaa atcgacattt acagatactt ttatagctct gtactgtgga acaacatcaa
     5221 aactattcag tattttctgg atagtttttt ttgtaccttg caaagactga ttggttggaa
     5281 ttttgaccat tatagttcta atgtattcat ttcgaattct agaaataggc ggttcttctg
     5341 gaccaagaat tggaatagta aaattttgct tcattacttg atacaaccac atagatcctt
     5401 ctttgagttt atcaaaatct ctatgtttca acgttaactt tataagttta taataaggtg
     5461 ggtaatgata aatttgcctg tcatacaact gctctttata catacctata taattattat
     5521 gaacaacctg ttgaatgatg ttatgatctg gattataagt ctgaataatt acttttcctt
     5581 gtttttcaga acgacctgaa cgccctgaaa cttgtgtcat catttggtaa cttcgttcga
     5641 aagccctaaa atcgggatga tacaacatat tatcggcatt cataatacct actaaactca
     5701 cgttatcaaa atccaaacct ttggcaagca tttgagttcc gaccaaaata tcaatttctc
     5761 tatttttgaa actatcaata attttttcaa aaccaaactt tcctcgagta gtgtcttgat
     5821 ccattcgacc aattttagta tttgaaaata actcgaccaa ttcttgttga atttgttctg
     5881 ttccaaaccc ttttgtagta agatgaacac tagaacaaga gtgacaatta gttggttttg
     5941 ccatcgaata tccacaataa tgacaacgca attgattttt atgtttgtga taagtcaaac
     6001 tcacatcgca ttgtggacat tgtggcacgt gtccacaggt caaacattcg attataggcg
     6061 aaaacccccg acggttttga aacaaaataa cttgttcgcc caaagacaaa gttttcgtaa
     6121 tttcctcaat caaaatatcg ctgaaatgtc ctgacatttg cttgcgaaaa tatttatctt
     6181 tcaaattgac caattcaatt tcgggcatct tgacattgcc gtatctttta gaaatttcga
     6241 ccaaaccata ttttcccgat tgggaattaa aataggtttc aatacttggc gttgccgaac
     6301 ccaaaagcac tttcgcctta tgactatttg ccaaaactat tgaagcatcg cgggcgtgat
     6361 agcgtggcga aggatcaact tgtttgaatg tttgctcgtg ttcttcatcg acaatgacca
     6421 atcccaaatt
//
LOCUS       DAOSNN010000154         6414 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12198_length_6414_cov_4.575719, whole genome shotgun sequence.
ACCESSION   DAOSNN010000154 DAOSNN010000000
VERSION     DAOSNN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12198_length_6414_cov_4.575719"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PLQ70_08400"
     CDS             complement(<1..814)
                     /locus_tag="PLQ70_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HQE34672.1"
                     /translation="MKRLLLLAFILIPSLFSKAQTTNYGVQFSNAILSRWPSGINSMT
                     GKGWEYSNSIVLLGMADIYDKTNDANYLTYIKTYVDGYIGADGTGVSSVLVQTLDNMH
                     PGILCLWLYEKTGALKYKTAATTIRDYLLKTGLGAYPRTPENGFWHKNNSSYTNVMMV
                     DGIYMASPFLVKYGALFNDTVAIDEAAFQTMILYSHVYNASNKLVYHAWNYDKTTYSW
                     SNPTTGVSSQVWSRGMGWYMMALIDILEYLPTNHARYPEMKTALQNLCEGLKN"
     gene            complement(979..1932)
                     /locus_tag="PLQ70_08405"
     CDS             complement(979..1932)
                     /locus_tag="PLQ70_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQE34673.1"
                     /translation="MKPPYDISPKILKLITAISEKIGEVNANYLTKQSPQLRKQNRIK
                     TIHSSLQIEGNTLTEEQITALIENKRVIGPEKEVLEVLNAIRVYEKLEDYKLDSDKSF
                     LKAHLELMQGLIESAGKYRKQGVGIMKGSKVEHVAPPYENVPYLMKDLFEYVKDSEEL
                     TLIKSCVFHYEMEFIHPFLDGNGRMGRLWQTLILMSEYPVFEFLPFETLISQTQNEYY
                     QALAMSDKSGKSTFFIEYMLEVIDKSLGNLLNYNNRVLKDIDRLDYFLKLGIKEFTRK
                     DYMNTFKDLSSATASRDLKKGVELQLFESSGTQNKTQYSVK"
     gene            complement(2084..3946)
                     /locus_tag="PLQ70_08410"
     CDS             complement(2084..3946)
                     /locus_tag="PLQ70_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQE34674.1"
                     /translation="MNYLSVENISKSFGERTLFKDISFGINKDQKIAFIAKNGSGKTT
                     IMNIINGFDEPDTGQVVLRKSIRMAFLSQDNNLQDELTIEESIFASDNETLKVIEAYE
                     KALENPEDEEAYQKAFDGMDQHNAWDFETQYKQILFKLKLEDFKLKVKNLSGGQKKRL
                     SLAIILINRPDLLILDEPTNHLDLEMIEWLESYFAKENITLFMVTHDRFFLERVCNEI
                     IELDNGKIYQYKGNYSYYLEKKEERIASENASVDKAQNLFVKELEWMRRQPKARTTKS
                     KSRQDDFYVIKEKAQSRRKENKVELEINMERMGSKIIELHKISKKFKDHVILDNFSFD
                     FQRGERIGIIGKNGTGKSTFLNLLTGTIPLDSGKVVVGETIKIGYYTQSGINPKPMQR
                     VIDVIKEYGEFIPLTKGRLISASQLLERFLFDSKKQYDYVEKLSGGELKRLYLCTVLI
                     QNPNFLILDEPTNDLDIVTLNVLESFLMDYPGCLLVVSHDRYFMDKIVDQLFVFRGQG
                     EIETFPGNYSDFRSYEDSADVAQKEDNKAEKKDWKQNNPTGNLTFNEQKEFQKIEKEI
                     KDLELDKAKIEQLFSDGKVADADIAGKAKELENIIKKIEEKEERWFELSAKIEG"
     gene            complement(4111..5067)
                     /locus_tag="PLQ70_08415"
     CDS             complement(4111..5067)
                     /locus_tag="PLQ70_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HQE34675.1"
                     /translation="MKKAILFFFLFCITSIYSQISGCMDSSAKNYNPNATIDNGSCRY
                     NSTKTKPETSIKLSDSLVETSGLIAFDHLLWTHNDDHVTTIYGLDANGKIQKKIKLEK
                     VKNNDWEEISQDENYIYIGDFGNNYQGNRRNLHILRIEKNSFLSNKPQIDTIAFSYSN
                     QTDFSVQKQNTTDFDCEAFVVSQDNIYLFTKQWSQNKTSIYALPKTPGNHIAQLKTTL
                     NVEGLITGATALSSGKEIVLCGYSRTLHPFLYLLYDYKNNDFSTGNQRKIKLSLPFHQ
                     IEGIATFDEKIFYLTNESFVRKPIINTPQQMHTVDLSLYLNH"
     gene            5165..6172
                     /locus_tag="PLQ70_08420"
     CDS             5165..6172
                     /locus_tag="PLQ70_08420"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQE34676.1"
                     /translation="MYHSKIAGLGFYVPENVVTNDDLSKIIDTNDEWIQERTGIQERR
                     HIISGEDTTTTMGVKAAKIAIERSGIAKEDIDFIVFATLSPDYYFPGPGVLVQRDLGL
                     KTVGALDVRCQCSGFVYAISVADQYIKTGMYKNILVIGSEVQSSGLDMTTRGRGVSVI
                     FGDGAGAAVLSREDDETKGILSTHLHSEGGHAEELVLKAPGMGGRWVTDILADNNPDD
                     ESYFPYMNGQFVFKNAVVRFAEVINEGLEANNLQVSDINLLIPHQANLRISQFIQKKF
                     RLTDDQVYNNVQKYGNTTAASIPIALTEAWEQGKIKSGDLVVLAAFGSGFTWGSVIIR
                     W"
BASE COUNT         1842 a         1169 c         1219 g         2184 t
ORIGIN      
        1 agtttttcaa accttcacaa agattttgca aagccgtttt catttcggga taacgcgcgt
       61 gattggtagg taaatattcc aaaatatcga tcaaagccat catataccaa cccattccgc
      121 gggaccaaac ttgtgaagaa acacctgttg taggattcga ccacgagtaa gttgttttgt
      181 cgtaattcca agcgtgatac actaatttgt tggaagcatt atacacgtgc gaataaagaa
      241 tcatcgtttg aaaagccgct tcgtcaatgg caaccgtatc gttgaagagt gcgccgtatt
      301 ttactaagaa aggcgatgcc atatagatgc cgtccaccat cattacgttg gtgtaagagg
      361 aattattttt atgccaaaaa ccattttcag gtgtacgagg ataagctcct aaacctgttt
      421 ttagaaggta atctcggatg gtagtcgcgg cagttttgta ttttaaggct cctgtttttt
      481 cgtacagcca caaacataaa ataccaggat gcatattgtc aagagtttgc accaataccg
      541 atgaaactcc tgtgccatct gcgccaatat aaccatcaac ataagttttg atgtaagtca
      601 gataattagc atcgttggtt ttgtcgtaaa tgtcagccat tcctaaaagt acaatgctgt
      661 tcgaatattc ccagcctttg cccgtcatac tgttaattcc agaaggccaa cgtgaaagaa
      721 tggcattcga aaattgcacg ccataattag tggtttgagc cttagaaaaa agggacggta
      781 ttagtataaa agcaaggagt aatagtcttt tcattttttt aatttcttgt tacaaagaaa
      841 gttaaaaaat gtaataaatc gcatagaatt aatacaaatt caggaaattt agtagatggt
      901 tattataaat ttctgtcttt tatacgtgct ttaatttctg cagttttttt gacttgggaa
      961 caaggttttc gaaggacttt attttacact atattgtgtt ttgttttgag ttccagaact
     1021 ttcaaaaagt tgtaattcta ctcctttttt taaatctcta ctggctgtag ccgatgataa
     1081 atccttgaaa gtattcatat aatcttttcg agtaaattct ttaatgccta gtttcaagaa
     1141 atagtccagt ctgtcaatgt ctttcaaaac cctgttgttg taattcagta aattacccaa
     1201 cgatttatca attacctcca gcatatattc aataaaaaaa gttgatttcc ccgatttgtc
     1261 actcatcgcc aaggcttggt aatattcatt ttgtgtttgg ctaatcaaag tttcaaacgg
     1321 cagaaattca aaaactggat attccgacat taaaattaaa gtttgccaca atcttcccat
     1381 tctgccgttt ccgtctagaa aaggatggat gaattccatt tcataatgaa aaacgcagct
     1441 ttttattaaa gtcaattcct ctgaatcttt aacatattcg aataagtctt tcatcaaata
     1501 cggaacattt tcataaggag gcgcaacgtg ttccactttc gaccctttca ttatgccaac
     1561 accttgcttc cggtatttac ccgcactttc gattaaacct tgcatcaatt caagatgcgc
     1621 tttcaagaaa cttttatccg aatccaattt ataatcttcc aatttttcat aaactcgaat
     1681 ggcgtttaat acttctaata cttctttttc gggaccaatc actctcttgt tttctatcaa
     1741 ggcggtaatt tgttcctcgg tcaatgtatt tccctcaatt tgcaaagaag aatggatggt
     1801 tttgattcgg ttttgttttc ggagttgtgg cgattgtttc gtgagataat tggcattgac
     1861 ttctccaatc ttttcagaaa tggcagtaat caatttcaat attttcggac taatatcgta
     1921 aggtggtttc attgatagta tcatttgata gtatcaaaag tagttttttt tatttgaaat
     1981 gacaattgtt tggaactatt gtagtttaat agatgtttgt tgaatttgtt tgtgggcaca
     2041 agcgtgacgc ttgcgccagc tggggaatct ttgcgaaaca attttatcct tctatcttag
     2101 cactcaattc aaaccatctt tcctctttct cttctatttt tttaatgatg ttttccagtt
     2161 ctttggcttt gccagcaata tcggcatcgg ctacttttcc atcggaaaat aattgctcga
     2221 ttttggcttt gtccaattcc aaatccttga tttctttttc gattttctgg aattcttttt
     2281 gctcgttgaa cgtcaggttt ccggttggat tgttttgttt ccagtctttc ttttcggctt
     2341 tgttgtcttc tttttgggca acatcggcac tgtcttcgta agacctgaaa tcggagtagt
     2401 tgccgggaaa agtttcaatt tcgccctgac ccctgaaaac aaataattga tccacgattt
     2461 tgtccataaa ataacggtcg tgcgacacca caagcaaaca acccggataa tccatcaaga
     2521 aactttctaa gacattcaag gttacaatat ccaaatcatt tgttggctca tccagaatca
     2581 ggaagttcgg gttttggatt aaaaccgtac acaaatacag tcgtttcaac tcgccaccgc
     2641 tcaatttttc gacataatcg tattgctttt tggagtcaaa aaggaaacgc tccaacaatt
     2701 gcgaagccga aatcaatctg cctttcgtca acggaataaa ttccccgtat tccttgatga
     2761 catcaatcac gcgttgcata ggttttgggt taatcccgct ttgggtgtaa tagccaattt
     2821 tgatggtttc gccaacgact acttttcccg aatctaaagg aatagttccg gtcaataaat
     2881 tcaagaaagt cgattttccg gttccatttt ttccaataat tccgatgcgt tcgccacgct
     2941 gaaaatcgaa actgaagttg tccaaaatca cgtgatcctt gaatttcttg gaaatcttgt
     3001 gaagttctat aatcttgctt cccatacgtt ccatattgat ttcgagttcc actttgttct
     3061 ccttgcggcg actttgggct ttctccttaa tcacataaaa atcatcctga cgcgatttcg
     3121 atttggtggt tctcgctttc ggctggcgac gcatccattc caattctttc acgaataggt
     3181 tttgagcttt gtccacacta gcattttcgg aggcaatgcg ctcttctttt ttctctaaat
     3241 aataagaata atttcctttg tattggtata tttttccgtt gtccaattcg atgatttcgt
     3301 tgcaaacacg ctccaaaaag aaacggtcgt gcgtcaccat aaacagcgtt atattttctt
     3361 tggcaaaata actttctagc cattctatca tctccaaatc caagtgattc gtgggttcat
     3421 ccagaatcaa caagtcggga cggttaatca aaatgatggc caatgacaaa cgtttttttt
     3481 gtccacccga aagatttttg actttcagtt tgaagtcttc caacttcaat ttgaacaaaa
     3541 tttgtttgta ttgggtttca aaatcccaag cattgtgttg gtccattccg tcgaaagctt
     3601 tttggtaggc ttcttcgtct tctgggtttt ccaatgcttt ttcataggct tcaatcacct
     3661 tcaaagtctc attgtccgaa gcaaaaatac tttcctcaat cgtcagttcg tcttgcaaat
     3721 tattgtcttg cgaaagaaaa gccattcgaa tgcttttgcg aagcacaact tgtcccgtat
     3781 cgggttcgtc aaaaccattg atgatgttca taatggtggt ttttcccgaa ccgtttttgg
     3841 cgataaaagc aattttttgg tctttgttga ttccgaagga tatgtcttta aaaagcgtgc
     3901 gctctccaaa agattttgag atattttcga ctgataggta attcacgagt gtaatttttt
     3961 tgcaaaagta agtttttaaa atggaagatg ctatggtgca acacaatcta ctctgtttgt
     4021 cacgctggaa gcgtctcaaa gtacgagagc aacataatcg agacgcttcc agcgtgacaa
     4081 acagcaagaa tagagaacta taaagaacta ttaatgattc aaatacaaac tcaaatctac
     4141 cgtatgcatt tgttgaggcg tattgataat gggttttcta acaaaggatt cattggttaa
     4201 atagaagatt ttttcatcaa aagtagcgat accttctatt tggtgaaatg gcagggaaag
     4261 ctttattttt cgctggtttc cagtcgaaaa atcattgttt ttataatcgt ataaaagata
     4321 taaaaacggg tggagtgttc ttgaatatcc acaaagcacg atttcttttc cagaagataa
     4381 agctgttgcg ccagttatca aaccctctac atttaaagtc gtttttagtt gagcgatatg
     4441 gtttccggga gtttttggca gggcataaat actcgttttg ttttggctcc attgtttggt
     4501 aaacaaatat atattgtctt gcgaaaccac aaaagcttcg caatcaaagt cagtcgtgtt
     4561 ttgtttttga acgctaaaat ccgtttgatt ggaataagaa aaagcaatag tatctatttg
     4621 aggtttattt gataaaaaag agtttttttc gattctaaga atatgtaaat ttcttcgatt
     4681 gccttgataa ttgttgccaa aatcgcctat gtaaatatag ttttcgtcct gcgaaatctc
     4741 ttcccaatca ttatttttta ctttttcgag tttgattttc ttttggattt ttccattggc
     4801 atccaatccg taaatggtgg tgacgtgatc gtcattgtgt gtccataata aatggtcgaa
     4861 agcaatcaaa cccgaagttt ctactaagct atcactgagt tttattgaag tttcaggttt
     4921 tgttttggta gaattgtaac gacaacttcc gttatcaata gttgcatttg gattataatt
     4981 tttggcactt gaatccatac aacccgaaat ttgactgtag attgaagtta tgcagaacag
     5041 aaagaaaaat aaaattgctt ttttcatttc gtcaaaagta agttttaaaa cgttcattat
     5101 aaaaataatt attatattag acttgatttt gaagaactat cagtttaaat aaaaataata
     5161 aaatatgtat cattcaaaaa tagcgggatt gggattttat gttccagaaa atgtggtcac
     5221 caatgacgat ttgtcgaaaa ttatagatac caacgacgaa tggattcagg aacgaaccgg
     5281 gattcaggaa cgaagacaca tcatttctgg agaagatacc accacaacaa tgggagtaaa
     5341 agcagccaaa atcgccattg aacgctctgg aattgccaaa gaagatatcg attttatagt
     5401 ttttgccact ttgagtccgg attattattt tcctggacca ggcgttttgg tgcaacgtga
     5461 tttgggcttg aaaaccgttg gcgctttaga tgttcgctgt caatgttcgg gttttgtgta
     5521 tgctatttcg gtggctgatc aatacatcaa aactggaatg tacaagaata ttttggttat
     5581 tggttccgaa gtgcaatcct caggattgga tatgacaaca cgaggtcgtg gggtttcggt
     5641 gatttttggt gatggggcag gagcagccgt tttgagtcga gaagatgacg aaaccaaagg
     5701 aattttatca acgcatttgc attcagaagg ggggcacgct gaggaattag tattaaaagc
     5761 tccgggaatg ggcggtcgtt gggttacgga tattttggca gacaataatc ctgatgacga
     5821 aagttatttt ccgtatatga acgggcaatt tgtgttcaaa aatgccgtag ttcgttttgc
     5881 tgaagtgatt aatgaaggtt tggaagccaa taatttgcag gtttccgata tcaatttatt
     5941 gattccgcat caagcgaatt tgaggatttc gcaattcatt caaaagaaat ttagattgac
     6001 tgatgaccaa gtgtacaata acgtccaaaa atacggaaat actaccgcag catctattcc
     6061 gattgccttg accgaagctt gggaacaagg aaaaatcaaa tcgggagatt tggtggtttt
     6121 ggccgctttt ggtagcggat tcacttgggg aagcgtgatt attaggtggt agttaattgt
     6181 tttaatttac cgcaaattcg caaattattt atgcagatcc agtctgttta atttgcgaat
     6241 ttgcggttta tttttggttt agtttgttag ccaaaataac aagcttaatt cattgttatc
     6301 gtttcaatgt aaaatgcgat ttgaagactt ttgaagttcc tttctcgtca taatctaccg
     6361 taaaccaata atcggtagag ggcaatggtt gtccgttgta cattccgttc cagc
//
LOCUS       DAOSNN010000155         6378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12289_length_6378_cov_6.397754, whole genome shotgun sequence.
ACCESSION   DAOSNN010000155 DAOSNN010000000
VERSION     DAOSNN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12289_length_6378_cov_6.397754"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLQ70_08425"
     CDS             <1..693
                     /locus_tag="PLQ70_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HQE34677.1"
                     /translation="DSIELFPASEGFFAYQDSQKEKGMLLLLNSGIFYEFIKSDEFYT
                     ENPKRHTIGEVELGVNYVLIISTNAGLWGYNIGDTVQFTSLKPYRVIVSGRIKHYISA
                     FGEHVIGKEVESALQEAIIDTNIRINEFTVAPQITPNEGLPYHEWFIEFENEPENFVA
                     FAEALDNAMRKQNIYYDDLIVGNVLRKVVITKVVKNGFQDYMKSIGKLGGQNKIPRLS
                     NDRKIVDLLERE"
     gene            701..1582
                     /locus_tag="PLQ70_08430"
     CDS             701..1582
                     /locus_tag="PLQ70_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQE34678.1"
                     /translation="MIKKRLFLVVVLFTCSISAQIKGVVKDSLSGKPIPFVNIWVEGE
                     NIGSTSEENGTFFINTTANGKKLIFSTLGYEKKIIKASSVMGVNLKPMAYSLNEVVIS
                     KSNGTKEIEIGKSKNEIYQAFDNGPKIDTKFFSYISSYKKTKYLKQVSIYTDSKIENA
                     SIKIHFYTVDSNGFPAEELLDKDFVVTVKKGTRINRFDLAKFNLKFPKNGLFVGFEKL
                     LIERNKTEKTITDLNTNITQVQKTYFPFVLYNYVEREFLYTFSGGKWNKQTNQNERDP
                     SPKMMINEPVITLILSN"
     gene            1626..3308
                     /locus_tag="PLQ70_08435"
     CDS             1626..3308
                     /locus_tag="PLQ70_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34679.1"
                     /translation="MKEMKNISRSRAQESSAAIEKMYITMRHLFNRGFYKPMGVSGDT
                     LREALLALRPEIYGNIAEEKVELSGLLYVIERLPTGIEECRFINLTSDEGYSKSHFQA
                     IVPPKRRRNCYRIDDEQMNVEITRGRSDIYDILTHLTFIFIESHKIKNRVLLDDAGEV
                     SRDWQKLEAAVSQKKKLTQIEKEKAVSHAANILGRTFIEVLDIYDAFGSELSPDRFLH
                     VIYWLGKLAIEEVVDDNKRTITFSPILRERLGHHIHGEIWATNIKEVLKQHKLLDRPI
                     HVISANMHSVMNSIFATTVLKTKFKDQSDFFIYEELSKSGANEVRNKVEEFAKQHGMI
                     SLPDAASGTNIDVQIFDTAKIDWAKSSFPNAKIGEKQPVLIVMDYAFGEQAYETIDEL
                     LKPYKKDILLNVESVSIMGKAGILQGGKGDIMIPSAHINEGTADNYYFENELKGDMFK
                     DDGIPVFEGPMVTVLGTSLQNKDLLKFFHESTWGVIGLEMEGSYYQKAIQSASKIRKS
                     IPQDVKVRYAYYASDNPLETGSTLASGGLGTTGVKPTYLITIKILEQIFNIK"
     gene            3352..4704
                     /locus_tag="PLQ70_08440"
     CDS             3352..4704
                     /locus_tag="PLQ70_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HQE34680.1"
                     /translation="MKTKTGRYSGYIRPFSYMIDLIIINVLAFYVFPFHDNKVVYSIL
                     ISFAWLSIAIYLGFYEVYRYTKVIAILNCALKQFILFSLFCLALELFYSDFYFQKRVV
                     FFVFVSIAFVLFFKLSIYCFLRKFRVLYGGNSRSVVLLGNMKNINPLKNFFTENPDYG
                     YRLMKVFTLEDRKKERIEECLSYVISSHTNEIYCAMADLSESQISKIVDFADNNLKTL
                     KFIPNENQIFSRTAKFEYYDYIPVISLRDILQDETINKIIKRVFDVVFSLLIIVGILS
                     WLTPILALLIKLDSKGPLFFVQKRNGLNYKEFNCYKFRSMYINDKADVDLASKNDVRI
                     TKIGKFIRKTSIDELPQFFNVLKGDMSVVGPRPHMVSVANLYAANVDKFMVRHFVKPG
                     ITGLAQTKGYRGEVETDEDIINRVKYDIFYMENWSILLDIQIIIKTIYNTLKGDEKAF
                     "
     gene            complement(4701..5897)
                     /locus_tag="PLQ70_08445"
     CDS             complement(4701..5897)
                     /locus_tag="PLQ70_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQE34681.1"
                     /translation="MNKILIISNYYPPEKGAAANRIEQLALKLHENKYNVTVLCPLGN
                     YPKGELFPEYKGNFSVTEKLQNITVKRLWIYPSVSKNIFKRTLSVLSFSSVLFFYLLF
                     KKIPHKVVVQSPPLLLSFVSVLILSLKRKKIILNVSDLWPLAAIELKALKKESFSHQV
                     SLFFERFIYSKASLIFGQSNEIITHIHSIFPEKECHLYRNFPDHQAKEIHFSTHENEP
                     IKLFYAGLLGVAQGVFELCQKIELEDLNIELHIFGDGAEKAQIEELIQQSRSLGTEKK
                     IFFHGMLERHILHEKLQTFDIAIIPLKTRIYGSVPSKIFEYGALGFPVLYFGGGEGET
                     IVNENNLGWVANVGNFESLNKALIEISKTGKSEIQMMKKQVFDTAQKQFNLNLQMKNL
                     IKEGVF"
     gene            complement(5984..>6378)
                     /locus_tag="PLQ70_08450"
     CDS             complement(5984..>6378)
                     /locus_tag="PLQ70_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HQE34682.1"
                     /translation="GDKKVIFPIHPRTKAILGETNLELKNILFVEPQGYLNFMYLIKN
                     SFAVITDSGGISEETTVMGIPCFTMRNNTERPETQIIGTNTLVGTSIENLEKLFGVFI
                     KNGKRQAGIPELWDGKASERIIEILLQK"
BASE COUNT         2156 a         1029 c         1145 g         2048 t
ORIGIN      
        1 gatagcattg aattgtttcc ggcttcggaa ggattttttg cttatcaaga ttcgcagaag
       61 gaaaaaggaa tgttgctgtt gttgaactcc ggaattttct atgaattcat caaaagcgac
      121 gaattttata ccgaaaaccc aaaaagacac accattggcg aagttgaatt gggcgtaaat
      181 tatgtcttga ttatttcgac caatgccgga ctttggggtt acaacattgg cgacaccgta
      241 cagtttacga gtttgaaacc gtatcgagtt atcgtttcgg gcagaatcaa acattatatt
      301 tcggcttttg gcgaacacgt tattggcaag gaagtcgaaa gtgctttgca ggaagctatt
      361 attgacacca atattcgtat taatgaattt actgttgcgc ctcaaatcac tccaaacgaa
      421 ggattgcctt atcacgaatg gtttatcgaa tttgaaaacg aacctgaaaa tttcgttgct
      481 tttgcagaag ctttggacaa cgctatgcgg aaacaaaata tctattacga cgatttgatt
      541 gtgggtaatg tgttgcgaaa agtggtcatc acaaaagtgg tcaaaaacgg ttttcaagac
      601 tatatgaaat ccattggaaa actgggcgga cagaataaaa ttccgaggtt gagcaacgac
      661 aggaaaatcg tagatttact agaaagagaa tagaacaaag atgataaaaa aaagactttt
      721 tttagtagtg gttttattca cgtgttccat ttcggcgcaa ataaaaggag tcgtcaaaga
      781 cagtctttct ggaaaaccca ttccgtttgt gaatatttgg gtggagggcg aaaacattgg
      841 ctcgacttca gaagaaaacg gcactttttt cattaataca actgcaaatg ggaagaaatt
      901 aattttttca actttgggtt acgaaaagaa aattatcaag gcttcttcag ttatgggagt
      961 caatttgaaa ccaatggcgt attccttgaa tgaggtcgta atttcaaaaa gcaatggcac
     1021 caaggaaatt gaaattggaa aatctaaaaa tgaaatatat caagcatttg ataatggacc
     1081 aaagattgac accaaatttt tttcttatat ttcgtcgtac aaaaagacaa aatatttaaa
     1141 acaagttagt atttacaccg acagcaaaat cgaaaatgcc agtattaaga ttcattttta
     1201 tacagtagat tcgaatggtt ttcctgccga agaattatta gacaaagatt ttgtggttac
     1261 agtcaaaaag ggaacaagaa tcaaccgatt tgatttagct aaattcaatt taaaatttcc
     1321 aaaaaacggt ctttttgttg gtttcgaaaa attgttaatc gaaagaaata aaaccgaaaa
     1381 aaccattacc gatttgaata cgaacattac acaagtacaa aaaacttatt ttccttttgt
     1441 tttgtacaat tatgtagaaa gagaattttt atatactttt tcaggcggaa agtggaataa
     1501 acaaacgaat cagaatgaaa gagatccatc acccaaaatg atgataaatg aaccagtgat
     1561 aacactaatt ttatctaact aaattattaa aaccatacat ttgtatggtt acaaagtaga
     1621 attttatgaa agaaatgaaa aacatatcaa gatctagagc acaagaatca tcggcagcta
     1681 ttgaaaaaat gtacatcaca atgcgccatt tgttcaaccg tggattttac aaaccgatgg
     1741 gtgtttcggg cgatacgttg cgagaagcat tattggcatt gcgaccagaa atttatggaa
     1801 acattgccga agaaaaagta gaattaagtg gactattata cgttatcgaa cgacttccaa
     1861 caggaattga ggaatgtaga ttcatcaatt taacttcgga cgaaggctat tcgaaatcac
     1921 attttcaggc tattgttcct ccaaaaagac gacgcaattg ttatcgaatt gacgatgaac
     1981 aaatgaatgt tgaaatcacc cgaggacgtt cggatattta tgatattttg acgcatttga
     2041 cctttatttt tattgaatcg cacaagatta aaaacagagt tttgcttgat gatgctggcg
     2101 aagtttcgcg cgattggcaa aaattagagg cagcagtttc acaaaagaaa aaactgactc
     2161 aaatagaaaa agaaaaagcc gtttcgcacg cagccaatat tttaggtaga acttttattg
     2221 aagttttaga catttatgat gcttttggat cagaattatc gccagatcga tttttgcacg
     2281 tgatttattg gttgggaaaa ttagccatcg aagaagtggt cgatgataac aagagaacca
     2341 tcacttttag tccgatttta agagaacgat taggacacca tattcacggc gaaatctggg
     2401 caaccaacat caaagaagtt ttaaaacaac acaaactttt agatcgacca atccatgtta
     2461 tcagtgccaa catgcacagt gtgatgaatt cgatttttgc tacaactgtg ttgaaaacta
     2521 agttcaaaga ccaatcggat ttctttattt atgaagaatt gagcaaatcg ggtgcgaatg
     2581 aagtcagaaa taaagtcgaa gaatttgcca aacaacacgg aatgatttcc ttgccagatg
     2641 ccgcttcggg aaccaatatt gatgtgcaaa tttttgacac ggccaaaata gattgggcta
     2701 aatcctcgtt tccaaacgct aaaataggag aaaaacaacc tgttcttatc gtgatggatt
     2761 atgcctttgg ggagcaggct tatgaaacca tagatgaatt attgaaaccc tacaaaaaag
     2821 atattttatt gaatgtcgaa tcggtttcta ttatgggaaa agcaggaatt cttcaaggag
     2881 gaaaaggcga tattatgatt ccatctgcac acatcaacga aggaactgcc gataattatt
     2941 actttgaaaa cgaattaaaa ggagatatgt ttaaagatga tggcattcct gtttttgaag
     3001 gacctatggt cacggtgctt ggaacatcat tgcaaaataa ggatttattg aagtttttcc
     3061 acgaatcgac ttggggtgta attggattgg aaatggaagg ttcttattat caaaaagcca
     3121 tccaatcggc ttcaaaaata agaaaaagta ttccacaaga tgtcaaagtt agatatgctt
     3181 actacgcttc ggataaccca ctcgaaacag gaagcacatt ggcttcagga ggtttaggaa
     3241 caacaggagt aaagcccacg tatttgatta ctattaaaat attagaacaa atttttaata
     3301 taaaataatc agggtttacg atttaaaaaa cttctaaaac acatcaaata ggtgaaaacc
     3361 aaaacaggaa gatattcagg ttatattcgt ccattttcat acatgattga tttaattatc
     3421 atcaatgtat tggctttcta tgtgtttccg tttcacgaca ataaagttgt ttattccatt
     3481 ctaattagtt ttgcctggtt gtcaattgcc atttatcttg gtttttatga agtctatcgc
     3541 tatacaaagg tgatagcaat tttaaattgc gctttaaaac aattcattct attttcctta
     3601 ttttgcttgg ctttagagtt gttttattct gatttttatt ttcaaaaaag agtcgttttc
     3661 tttgtttttg tatcgatagc atttgtttta tttttcaaac tttccattta ttgttttcta
     3721 agaaaattta gagttttata tggaggaaat tccagaagcg ttgttttatt gggaaacatg
     3781 aaaaacatca atccactaaa aaactttttc acagaaaatc cagattatgg ttacagacta
     3841 atgaaagttt tcactctaga agaccgtaag aaagaaagaa tcgaagagtg cctttcctat
     3901 gtaatttcta gtcataccaa tgaaatatat tgtgcgatgg ccgatttgtc agaaagccaa
     3961 attagcaaaa ttgttgattt tgccgataat aatttgaaga ctttaaaatt tattccaaac
     4021 gaaaaccaaa ttttttcaag aacagcaaag ttcgaatatt atgactatat tccagttatt
     4081 tctttgagag atattttaca ggatgaaacc ataaataaaa taattaaacg cgttttcgat
     4141 gttgtttttt cattattaat aattgtcgga atattgtctt ggcttacacc aattttagcc
     4201 ctattaataa aattagattc aaaagggcct ttgttttttg tacaaaaaag aaatggtctt
     4261 aattataaag aatttaattg ttacaaattt cgttcgatgt atataaacga taaagccgat
     4321 gtggatttgg cttcaaaaaa tgatgttcga attaccaaaa taggcaaatt tattaggaaa
     4381 actagcattg atgagttgcc tcagtttttc aatgttttaa aaggtgatat gtcggttgtt
     4441 gggcctagac cacatatggt tagcgttgct aatttgtatg cagccaatgt cgataaattt
     4501 atggttcgtc attttgtaaa accaggcatc accggtttgg ctcaaaccaa agggtatcgt
     4561 ggcgaggtag aaaccgatga agacatcatt aatagagtaa aatacgatat tttttatatg
     4621 gaaaactggt ctattttatt agatattcaa atcattatca aaactattta taatactttg
     4681 aaaggcgatg aaaaagcttt ttaaaaaaca ccttctttaa ttaagttctt catttgtaaa
     4741 tttaaattaa actgtttttg ggcagtgtca aagacctgtt ttttcatcat ttgaatttca
     4801 cttttcccag tttttgatat ttcgattaaa gccttattta aactttcaaa attgcccaca
     4861 ttcgctaccc agcctaaatt attttcgttt acaatagttt cgccttcgcc accaccaaaa
     4921 tacaatacag gaaaacccaa agccccatat tcgaatattt tagaaggaac agagccataa
     4981 attcttgttt ttaatggaat aatggcaata tcaaaagttt gtagtttttc gtgcagaata
     5041 tgacgttcca acattccgtg aaagaagatt tttttctcgg tcccgaggct tcgggattgt
     5101 tgaatcaact cttcaatttg tgctttttcg gcaccatcgc caaaaatatg caattcaatg
     5161 tttagatctt ctaattctat tttttgacac aattcaaaaa caccttgggc aacacccaat
     5221 aatcctgcat aaaatagctt gataggttcg ttttcatggg ttgaaaaatg aatttctttg
     5281 gcttgatgat ccggaaaatt gcggtacaaa tggcattctt tttcaggaaa aatagaatga
     5341 atgtgcgtga taatttcgtt cgattgacca aagattaaac tagctttcga ataaataaat
     5401 cgttcaaaaa acaatgaaac ttgatgcgaa aaactttctt ttttcaaggc tttcaattca
     5461 atagccgcca gtggccacaa atcggaaaca ttgaggatta ttttttttcg cttaagcgaa
     5521 agaattaaaa ccgacacaaa cgacaacaac aacggaggcg attgcaccac cactttatgc
     5581 ggtatttttt tgaacaataa ataaaagaaa agcaccgaag aaaacgacaa aaccgacagc
     5641 gttctcttga aaatattttt gctcacactt ggataaatcc aaagcctttt taccgtaata
     5701 ttttgcaatt tttccgttac cgaaaaattg cctttgtatt ctggaaatag ttctcctttt
     5761 gggtaatttc ctaacggaca aagcacggta acgttatact tattttcgtg taatttcagt
     5821 gccaattgct caattctatt ggcggcagcc cctttttcgg gcggataata attggatatg
     5881 atgaggattt tgttcatttt tttaccgcaa attcgcaaat ttatttttta attaacagcc
     5941 aaataaacaa ttttatgttt taataatttg cgaatttgcg gttttatttt tgaagtagaa
     6001 tttcaataat tcgttccgag gcttttccgt cccaaagttc tggaattcct gcttgtcgtt
     6061 ttccgttttt aataaatact ccaaacagtt tttctaaatt ttctatcgaa gtccctacta
     6121 aagtatttgt tccaattatt tgtgtttcag gtctttcggt gttgtttcgc atcgtaaaac
     6181 aaggaattcc catcacggta gtttcttccg aaattccacc cgaatccgta attacggcaa
     6241 aactattttt gatgaggtac ataaagttta aataaccttg tggctctaca aaaagaatgt
     6301 ttttcagttc taaattggtt tcgcccaaaa tcgctttcgt tctgggatga atcgggaaaa
     6361 tcactttttt gtcgcctg
//
LOCUS       DAOSNN010000156         6270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12554_length_6270_cov_4.528560, whole genome shotgun sequence.
ACCESSION   DAOSNN010000156 DAOSNN010000000
VERSION     DAOSNN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12554_length_6270_cov_4.528560"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLQ70_08455"
     CDS             <1..217
                     /locus_tag="PLQ70_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQE34683.1"
                     /translation="EGLGNEFEVGRYHSWVVDANLPDVLEATSFDENGQVMSLRHKTY
                     DVRGVQFHPESVLTPNGKKILENWLKS"
     gene            426..1148
                     /locus_tag="PLQ70_08460"
     CDS             426..1148
                     /locus_tag="PLQ70_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQE34684.1"
                     /translation="MVLDLLDLQANNKLGKDTLVTIVNDPVYHKAKKYKAVSASLLVK
                     TEIELNKIDSKNTKIVFECVDGYKPEMPLELFLNANPFLAFEDVDAPKGSKWEPILKE
                     GNEMNAEPFYIVYPSISEKDSRYKWPYNLVKIYLEPLNKSTLELYPLKNKKLETGYTL
                     FKNQCLTCHSINGIGGTMGPELNYPKSVTEYWKENELVNYIVNPASFRNKVKMPTLGI
                     TKQQSQEIVDYLKYMSENKKKI"
     gene            1149..2141
                     /gene="trpD"
                     /locus_tag="PLQ70_08465"
     CDS             1149..2141
                     /gene="trpD"
                     /locus_tag="PLQ70_08465"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQE34685.1"
                     /translation="MKAILNRLINHETLSKEEAKNVLVNISNGSYNPSQIAAFLTVYM
                     MRSISIDELAGFREALLELCVRVDLSTYNTIDLCGTGGDGKDTFNISTLASFVAAGAG
                     IKVAKHGNYGVSSISGSSNVMEKLGVKFSNNNDFLEKCIDQAGICILHAPLFHPAMKN
                     VGPIRKELGVRTFFNMLGPMINPSFPQNQLVGVFNLELARKYAYLYQNTDMNFTILHS
                     LDGYDEVSLTCPTKTITKRMEGVLKPEDFGVRQLLQSEIEGGKTIEESAAMFMNIISG
                     KGNEAQNNVVCANAGMAIATVNGSSPLESFQLAKESLLSGKALTALNKLQELSR"
     gene            2217..2999
                     /gene="trpC"
                     /locus_tag="PLQ70_08470"
     CDS             2217..2999
                     /gene="trpC"
                     /locus_tag="PLQ70_08470"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQE34686.1"
                     /translation="MNILDRIIIDKKKEVILKKSIIPVSQLEASVFFGKKTISLSNNL
                     RNSNSGIIAEHKRRSPSKAEINYGFTVEEVVKGYESAGACGISVLTDGKYFGGSLDDL
                     LLARASVNIPLLRKEFIVDEYQILEAKAHGSDLILLIAAVLTREEIKSLSEFAKSLGL
                     EVLLEVHNQEELEKSIMPTLDMIGVNNRNLKTFEVSLDFSKELANQIPNDFVKVSESG
                     ISSIEAINELKPYGYKGFLIGENFMKTDNAGKAATEFIKQLK"
     gene            3110..3676
                     /locus_tag="PLQ70_08475"
                     /pseudo
     CDS             3110..3676
                     /locus_tag="PLQ70_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
     gene            3725..4906
                     /gene="trpB"
                     /locus_tag="PLQ70_08480"
     CDS             3725..4906
                     /gene="trpB"
                     /locus_tag="PLQ70_08480"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQE34687.1"
                     /translation="MSYNVNEKGYYGEFGGAYIPEMLYPNVEELRQKYLEITAQPEFK
                     AEFDQLLKDYVGRPSPLYFAKRLSEKYNTKVYLKREDLNHTGAHKINNTIGQILVAKK
                     LGKKRIIAETGAGQHGVATATVCALMGLECIVYMGEIDIARQAPNVARMKMLGAEVRA
                     ALSGSRTLKDATNEAIRDWINNPVDTHYIIGSAIGPHPYPDMVTRFQSVISEEIKWQL
                     KEKEGRENPDYVVACIGGGSNAAGTYYHFLHEPEVSIIAVEAAGKGVNSGHSAATSKL
                     GKVGVIHGCKTLLMQTPDGQITEPYSISAGLDYPGVGPMHAHLAQTGRAEFFSVTDDD
                     AMKAGLELCKLEGIIPAIESSHALAVLNDKKFKPEDIVVISLSGRGDKDLNTYIDYFK
                     L"
     gene            4929..5261
                     /locus_tag="PLQ70_08485"
     CDS             4929..5261
                     /locus_tag="PLQ70_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HQE34688.1"
                     /translation="MIQLFTYGTLQHDDVQENLFGRVLTGTPEKLIGYVLKRIQIEEE
                     FGIVQYPVIVETNNEADFIDGIVYELTEQELNQADLYEGLHYKRVEVHLHSNQKAWAY
                     SSADFSHY"
     gene            5312..6073
                     /gene="trpA"
                     /locus_tag="PLQ70_08490"
     CDS             5312..6073
                     /gene="trpA"
                     /locus_tag="PLQ70_08490"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQE34689.1"
                     /translation="MNRINQKLQENKKILSIYFSAGYPSLNDSVQIIQDLEKNGVDMI
                     EIGLPFSDPLADGPTIQASSTQALQNGMTTQFLFDQLKDIRKTVSIPLVIMGYFNPML
                     QYGVEAFCQKCAEIGIDGLIIPDLPVDVYADEYKATFDQYGLKNIFLITPQTSDERIR
                     FIDSVSDGFIYMVSSASVTGSQSGFGSTQEDYFKRIADMNLKNPQIIGFGISNQETFN
                     QATQFAKGAIIGSAFIKHLSEEGTSKIGEFVKGIR"
BASE COUNT         2172 a         1070 c         1237 g         1791 t
ORIGIN      
        1 tgaaggttta ggaaatgaat tcgaagtggg acgctatcat tcctgggtgg ttgatgccaa
       61 tttaccagat gttttggaag ccacttcatt tgacgaaaac ggacaagtaa tgtctttgcg
      121 ccacaaaaca tacgatgttc gcggcgtgca atttcatccg gaaagtgtgt tgacaccaaa
      181 tggaaagaag attttggaga attggttgaa atcgtagaga cgttgcaatg caacgtctgt
      241 acattgttaa tcaaaaaaca ataaaaaaga aacgttgcac tgcaacacgt ccctacctta
      301 cggtaaataa aatgaaaaca gctaaaatcc atcttatact ctcttgtctt tttattttta
      361 gcatcctttc ttgctattaa aagaaggagc aaaaaataga aaccacgagt atttcccaac
      421 ccgaaatggt tttggattta ttggatttac aggcaaataa caaattagga aaagatacgc
      481 ttgtgaccat tgtgaatgat cctgtttatc acaaagcaaa gaaatacaaa gccgtgagtg
      541 cgtcgctttt ggttaaaact gaaattgaat taaacaaaat cgacagcaaa aacaccaaaa
      601 tagtgtttga atgtgtggat ggttacaaac ctgaaatgcc tttggaattg tttttaaacg
      661 ccaatccgtt tttggcattt gaagatgttg acgcgccaaa aggaagcaaa tgggaaccta
      721 ttctaaagga agggaacgag atgaatgctg aaccatttta cattgtttat ccttcgattt
      781 cggaaaaaga ttcgcgttac aaatggcctt ataatctggt gaaaatctat ttggaaccgc
      841 tgaataaatc aactttggaa ttgtatcctc tgaaaaacaa aaaattggaa acgggctata
      901 cattattcaa aaatcagtgc ctaacttgcc attcgataaa cggcatcgga ggaacaatgg
      961 gaccggaatt gaattatccg aaaagcgtga ccgaatattg gaaagaaaac gaattggtaa
     1021 attacattgt gaatccggct tctttcagaa acaaggtaaa aatgcctaca ctcggaatca
     1081 ccaaacaaca atcccaggaa attgttgatt atttaaaata tatgtctgaa aataaaaaga
     1141 aaatataaat gaaagccata ctaaaccgat tgatcaacca cgaaacactt tctaaagaag
     1201 aagccaaaaa cgttttggtc aatatctcca acggaagtta taatccgagt caaattgctg
     1261 cttttcttac cgtttatatg atgcgaagca tcagtattga tgaattggcc ggttttcgcg
     1321 aagccttgct ggaattgtgt gttcgcgtgg atttatccac ttacaacact atcgatttgt
     1381 gcggaactgg gggcgatgga aaagacactt tcaacatatc cactttagct tcttttgtgg
     1441 cagctggagc aggaatcaaa gtggcgaaac atgggaatta tggggtttcg tctatttcgg
     1501 gttccagcaa tgtaatggaa aaactaggcg taaaattcag taataataat gattttttgg
     1561 aaaaatgtat tgatcaggct gggatttgca ttttgcacgc acctttgttt caccctgcaa
     1621 tgaaaaacgt gggccccatt agaaaagaat taggtgtgag aacctttttc aatatgctcg
     1681 gaccaatgat aaatccttct tttcctcaaa atcaattggt aggtgttttc aatttagaac
     1741 tcgccagaaa atatgcctat ttgtatcaaa acaccgatat gaattttacc attttacatt
     1801 cgctggacgg gtatgatgaa gtatctttga cctgcccaac gaagaccatt acgaaaagaa
     1861 tggaaggcgt gctgaaacca gaagattttg gcgttcgaca attattgcaa agcgaaatcg
     1921 aaggcggaaa aaccatagaa gaatcggcag caatgtttat gaatatcatt tccggaaaag
     1981 gaaatgaagc tcagaataat gtggtttgtg ccaatgcggg aatggctatt gcaaccgtaa
     2041 atggaagttc gcctttggaa agttttcagt tggcaaaaga aagtttgctt tctggaaaag
     2101 cattgacagc attgaataaa ttacaagaat taagtagata gaaataaaca cgaatttcac
     2161 gaattagcac aaattatttt tttgcttctt tgtgactttg tggcaaaata aataaaatga
     2221 atattttaga cagaatcata atcgacaaaa aaaaagaagt tattctcaag aaatccatca
     2281 ttcctgtttc gcaattggaa gcttcggttt tctttggaaa aaagactatt tctttgagca
     2341 acaatttaag aaacagcaac tctggaataa tcgctgaaca caaacgccgt tcgccttcca
     2401 aagctgaaat caattatggt tttacggtcg aagaagtggt aaaaggatat gaaagtgcag
     2461 gtgcttgcgg aatttcggtt ttgaccgacg gaaaatattt cggcggttca ttggacgatt
     2521 tactgttggc cagagcttcg gtaaacattc cgttattacg aaaagaattc attgtggatg
     2581 aataccaaat attggaagcc aaagcccacg gatccgattt gattttatta atcgcagcag
     2641 ttttgactcg cgaggaaatc aaatcattat cagagtttgc caaaagttta ggattggaag
     2701 ttttattgga agttcacaat caggaagaac tggaaaaatc aattatgcct acattagata
     2761 tgattggcgt gaacaacaga aatctgaaaa ctttcgaggt tagcctagat ttcagcaaag
     2821 aattggccaa ccaaattccg aatgatttcg tgaaagtctc cgaaagcggc atttcgtcca
     2881 ttgaagcgat taacgaatta aaaccatatg gttacaaagg cttcttgatt ggagaaaact
     2941 ttatgaaaac cgacaatgct ggaaaagcag caacagaatt tattaagcaa ttaaaataag
     3001 acacaaattt cacaaattta cacgaatcaa atctgtgacc caagcgacag caaataggcg
     3061 aagcaaatct atggcaaaaa ctaaaactat gaaactcaaa atctgcggaa tgaaataccc
     3121 cgaaaacatg ctcgaagtag gcgagctcct acccgattat atgggtttta tattctggga
     3181 gaaatcggca cgttattttg atggaataat tcccgaactg cctaagtcta ttaagaaagt
     3241 aggcgttttt gtcaatgctt ctttagaaga aattctaaag aaaattgaaa tgcacgattt
     3301 gcaagccatt cagcttcacg gtaccgaatc tgttgaattt tgtgaaaatt taaaaaaaaa
     3361 tataccaaac ctgatagata tcataaaagt attttcaatt ttagatacct ttgactttga
     3421 tttgctcaaa ccatacgaaa aagtgtgtga ttattttctt tttgacacca aaggaaaatt
     3481 gcctggagga aacggaacct cttttgattg gaaagtttta gaaaaatacc cttcgacgaa
     3541 gccgtttttc ttgagtggcg gaatcggaat ggataatata gaggccataa atgaaatttt
     3601 gaaaacgaat ttacccattt atgccattga tgtaaacagt aaatttgaaa ttgaacctgg
     3661 attaaaaaat atacaattat gcagagacgc aacgcattac gtctctacaa ttaaaaaaat
     3721 aaaaatgtca tacaacgtta acgaaaaagg ctattacgga gaatttggag gagcatatat
     3781 ccctgaaatg ttatatccaa atgtggaaga attacgtcaa aaatatcttg aaatcacggc
     3841 tcaacccgaa tttaaagccg aatttgacca attgctaaaa gattatgtag gtcgcccaag
     3901 tccgttgtat tttgccaaaa gactttctga aaaatacaac accaaagtct atctgaaaag
     3961 agaagatttg aatcatactg gagcacacaa aatcaacaat actattggtc agattttagt
     4021 agccaaaaaa ctaggcaaaa aaagaatcat tgccgaaact ggagcaggtc agcacggagt
     4081 ggccacagct accgtttgtg ctcttatggg attggaatgc atcgtgtata tgggtgaaat
     4141 tgacatcgct cgtcaggctc caaatgtagc tcgaatgaag atgttgggcg ctgaagttcg
     4201 tgcagcactt tctggttcaa gaacgttaaa agatgctact aatgaagcca ttcgcgattg
     4261 gattaacaat cctgtcgata cgcattacat cattggttca gccattggtc cacatcctta
     4321 tcctgatatg gtaactcgtt ttcagagtgt tatttctgaa gaaattaaat ggcagttaaa
     4381 agaaaaggaa ggacgtgaaa accctgatta tgttgtagct tgtatcggtg gcggaagtaa
     4441 tgcagcagga acctattacc actttttgca cgagcccgaa gtgagtatta tagctgtaga
     4501 agccgctgga aaaggagtca attctggtca tagtgctgcc acaagtaaac tgggaaaagt
     4561 aggtgtcatt cacggttgca aaaccttatt gatgcaaact cccgacggtc aaataaccga
     4621 accctattct atttcggctg gattggatta tcctggagtt ggaccaatgc acgcacattt
     4681 ggcgcaaacc ggtcgtgccg aatttttctc tgtaaccgat gacgatgcta tgaaagcagg
     4741 attagaactt tgcaaactcg aaggtataat tcctgctatt gaatcttcac acgctttggc
     4801 ggtcttaaat gacaagaaat tcaaacccga agatattgtc gtaatcagcc tttcgggtcg
     4861 tggcgacaaa gatttgaaca cctatataga ttattttaaa ttataattga cgttaaaaaa
     4921 agcatgctat gatacaatta ttcacctacg gaactttgca acatgatgat gtacaagaaa
     4981 acctttttgg acgtgttcta accggaactc ccgaaaaatt gattggatat gttttaaaga
     5041 gaattcagat cgaagaagaa tttggaatag ttcaatatcc agttattgtg gaaactaata
     5101 atgaagcgga tttcatagat ggaattgttt atgaacttac agaacaagaa ttgaatcaag
     5161 cagatttgta tgaaggattg cattacaaaa gagttgaagt acatttgcat tcgaaccaaa
     5221 aagcttgggc atacagttct gccgactttt cgcactatta aaatattttt taaaattcaa
     5281 atagcaacat tgcactgcta caaacataaa aatgaacaga ataaatcaaa aattacaaga
     5341 aaacaaaaag atactttcca tctatttttc ggcaggatat ccaagcctaa acgatagcgt
     5401 gcaaatcatt caagatttag aaaaaaacgg ggttgatatg atagaaattg ggttgccttt
     5461 tagcgatcct ttggccgatg gccccacgat tcaagccagt tctactcaag ctttgcaaaa
     5521 cggaatgact actcaatttt tgtttgacca actcaaagat attcgaaaaa ctgtctccat
     5581 tccgttagtg ataatgggat attttaatcc aatgttacaa tatggtgtag aagctttttg
     5641 ccagaaatgt gccgaaattg gtattgatgg cttgatcatt cctgaccttc cggttgatgt
     5701 gtatgctgac gaatacaaag ctactttcga ccaatacgga ctcaaaaata tctttttgat
     5761 tacaccacaa acttcggatg aacgcattcg ttttattgac agcgtttcgg atggatttat
     5821 ctatatggtg agttcggcta gtgttacagg ttcacaatcg ggttttggaa gcacacaaga
     5881 agactatttt aaacgcattg ccgatatgaa cctgaaaaat ccacaaatta ttggttttgg
     5941 aattagcaat caggaaacgt ttaatcaagc cactcaattt gcaaaaggtg cgattatagg
     6001 aagtgctttt atcaaacatt tatctgaaga aggcacttca aaaattggag aatttgtaaa
     6061 gggaattcga taattgctat ttgcaaaatg tttagtaaaa aaaatattcc ctaatttttc
     6121 aactagggaa tattttaaac taacactaac tcaaaaaaaa aaatcattta ataaagccca
     6181 tatcatcaat ataaactgtg ttttttccac aggtaaattc ttgaataata aaattttgtg
     6241 ttgaattatt gacattagtt gttaattttt
//
LOCUS       DAOSNN010000157         6259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12591_length_6259_cov_4.310445, whole genome shotgun sequence.
ACCESSION   DAOSNN010000157 DAOSNN010000000
VERSION     DAOSNN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12591_length_6259_cov_4.310445"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1446
                     /locus_tag="PLQ70_08495"
     CDS             103..1446
                     /locus_tag="PLQ70_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQE34690.1"
                     /translation="MEFTLLIVIIVLALIFDYINGFHDAANAIATVVATKVLTPFQAV
                     LWAAFFNFLAYWVFGFGVADTVAKTAHTMDINLIVILAGVIAAICWNLLTWWLGIPSS
                     SSHTLIGGFAGAALAHAIAVHGFSGYETADGTQHWYNIVSWYKAGKDGGLPSGVIIII
                     LFIVLAPLVGAIASYLISIWLLNASKKRILPKLFTIALMVGTVLFVYFQMVPYDKIEK
                     PRFDSHLWSVVFEAHNIKWFLVAFIILSISTFALTFSSLSLHKADSVLKKMQLLSSAA
                     FSLGHGGNDSQKVMGIIAAAVAVYIHTTGQTQESMDAWLNVILPNEDLDIKGSMPAWI
                     PLACYSAIAAGTLSGGWKIVKTMGSKITKVSSFEGVAAETAGALTLYFTEHLKIPVST
                     THTITGSIIGVGLTKRISAVRWGVTVSLLWAWVLTIPVSAILAAIVYYVLSIFLG"
     gene            complement(1518..2774)
                     /locus_tag="PLQ70_08500"
     CDS             complement(1518..2774)
                     /locus_tag="PLQ70_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQE34691.1"
                     /translation="MTETKKTSFLGNLTVQIVIAMLLGAFLGIFVHHNYDATFAKEFS
                     DKIKILATVFIRLVQMIIALLVFSTLVPGIAKLGDVKTVGRVGGKALGWFFSASFISL
                     LIGLFWVNVLQPGVGLNLSNIDLSTAAEVTGNTKSFSTQNFVEHIIPKSIVEAMANNE
                     IIQIVIFSIFFGLAAASIGTYAKPVTHFIEVLSHIVLKMVNYVMKFAPMGVFGAIAGV
                     FAIKDLNELVFTYIKFFGSFLVGIGSLWIFLLAIGFLFLGKRMKTLLNHIVGPLIIAF
                     STTSSEAVFPKLTEELERFGIKDKIVAFMLPLGYSFNLDGSMMYMTFASLFIAQAYGI
                     DLDMGTQMTMLLVLMLTSKGIAGVPRASLVIVAATCGMFKIPIEGIALILPIDHFCDM
                     FRSATNVLGNALATSVVGKWEGEDSE"
     gene            complement(2851..3915)
                     /gene="aroC"
                     /locus_tag="PLQ70_08505"
     CDS             complement(2851..3915)
                     /gene="aroC"
                     /locus_tag="PLQ70_08505"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQE34692.1"
                     /translation="MAGNSYGTLFRITTFGESHGEALGGIIDGCPSGIALDFDAIQLE
                     MSRRKPGQSKIVTQRKEPDDVQFLSGIFEGKTTGTPIGFIIPNTNQKSDDYSHIKDNY
                     RPSHADYVYEKKYGVRDYRGGGRSSARETASRVVAGAIAKQVLPEIKINAYVSSVGPL
                     FLEKPYQELDFSKIESNPVRCPDEKMAAEMEEYIKVIKKDGDTVGGTVTCVIQNVPIG
                     LGEPVFDKLHAELGKAMLSINAVKGFEFGSGFCGAKMKGSEHNDLYNPDGTTRTNLSG
                     GIQGGISNGMDIYFRVAFKPVATIMQKQESLDNQGNITEMTGKGRHDPCVVPRAVPIV
                     EAMAALVLADFYLINKTYFK"
     gene            4088..4468
                     /locus_tag="PLQ70_08510"
     CDS             4088..4468
                     /locus_tag="PLQ70_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQE34693.1"
                     /translation="MKKIIFTEKSPAPIGPYNQAVLKGNTLYTSGQIAINPATGELVV
                     SDIESETKQVMENMKAVLEAAGMTFENVVKSTIFIMNMNDFGTINSVYGSYFDEKTAP
                     ARETVQVAGLPKNVNVEISMIAIL"
     gene            complement(4458..4829)
                     /locus_tag="PLQ70_08515"
     CDS             complement(4458..4829)
                     /locus_tag="PLQ70_08515"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HQE34694.1"
                     /translation="MEIAIIAHDGKKADMVQFLNKNKTVLQREDIKLIGTGTTGSKAE
                     SAGFKVKKMLSGPQGGDAQIAGRVAEGKTKMVLFFKDPLSSHAHESDVNMLLRVCDVH
                     NVPLATNEASAQLFIDAIAIK"
     gene            complement(4860..5711)
                     /locus_tag="PLQ70_08520"
     CDS             complement(4860..5711)
                     /locus_tag="PLQ70_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HQE34695.1"
                     /translation="MRLLVDSGSTKADWIAIDEDGKILFTTQTLGLNPEILDGNEIVE
                     RLNDRFDILQNKEKATHLFFYGAGCGTDRMKIDLSRIFQDYFPNAIVDVQEDTYAAVY
                     ATTPKGEEAIVSILGTGSNCSYFDGKVLHQKVQSLGYIVMDDCSGNVLGKELIRKYYF
                     NKMPKELAVEFEKEYDLDPDFIKNKLYKEPNPNAYLATFAKFLIQNKEHPFCQKIIFK
                     GMKSFIKNYVQQYDNCKEVPVHFVGSIAFYLKEELQVMFDKYEMKLGNVLRRPIDGLI
                     AYHVANK"
     gene            complement(5821..>6259)
                     /locus_tag="PLQ70_08525"
     CDS             complement(5821..>6259)
                     /locus_tag="PLQ70_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQE34696.1"
                     /translation="PSRKDWRGGRAAAINIIPSSTGAAKAVGKVIPSLNGKLTGMSFR
                     VPTADVSVVDLTVKVAKETSYEEIMAVLKNASETSLKGILGFTEDAVVSQDFISDKRT
                     SIIDATAGIGLNSTFFKLVSWYDNEYGYSSKLIDLAVHITSLK"
BASE COUNT         1956 a         1144 c         1002 g         2157 t
ORIGIN      
        1 aaacattcgt aaccaattta aaaaaattaa acagttgtaa aggttttttt ttagaattga
       61 accccattca attgtttgat ttccaaattt tcaattaaaa atatggaatt tactctactc
      121 atagttatta tcgtattggc tttgattttt gattacatca atggttttca tgatgctgcc
      181 aatgctattg ctactgttgt tgcaaccaaa gttttgactc cttttcaagc cgtactttgg
      241 gctgcttttt ttaacttttt ggcctattgg gtttttggtt ttggtgttgc agatactgtc
      301 gctaaaaccg ctcatacaat ggatatcaat cttatagtta tccttgctgg agttattgcg
      361 gctatttgct ggaatttatt gacttggtgg ttaggaattc catctagttc atctcacact
      421 ttgataggtg gatttgctgg agctgctctg gctcatgcta ttgcagttca cggatttagt
      481 ggttatgaaa cggccgatgg aactcaacat tggtataata ttgtaagctg gtacaaggca
      541 ggaaaagatg gtggattgcc ttcgggagtt atcatcatta tactatttat tgttttagca
      601 cctcttgttg gagcgattgc ctcctattta atttcgattt ggttgttgaa tgcttccaaa
      661 aaaagaatac tcccaaaatt atttactatt gcattaatgg ttggaactgt tttatttgtc
      721 tattttcaaa tggttcctta cgataaaatc gaaaaaccac gttttgattc tcatttatgg
      781 agtgttgttt ttgaagcaca taatatcaaa tggtttttag ttgcttttat tattttatcc
      841 atttctactt ttgctttaac ttttagtagt ttgagtttgc ataaagctga ttctgttttg
      901 aaaaagatgc agttattgtc ttcggcggca tttagtttgg gacatggagg aaatgattca
      961 caaaaagtaa tgggaattat agcggcagcc gttgctgttt acattcatac tacagggcaa
     1021 actcaagaaa gcatggatgc ttggttaaat gtgattttac ctaatgaaga tttagatata
     1081 aaaggttcta tgccagcttg gattcctctt gcttgctatt ctgccattgc agctggcacc
     1141 ttgagtggag gttggaaaat tgtaaaaaca atgggatcca aaatcaccaa agtatcttca
     1201 tttgaagggg ttgctgcaga aactgctgga gcattaacat tatattttac agaacattta
     1261 aaaattcctg taagtacgac tcacactatt acaggttcaa taattggagt tggattgacc
     1321 aaaagaattt cggctgtacg ttggggtgtt acagtaagcc ttttatgggc ttgggtactt
     1381 acaattcctg tttcggcaat attagcagca attgtttatt atgttttgag tatcttttta
     1441 ggataaaata ttttacatat aaaaaaactc attcgttatt gaatgagttt ttttttatat
     1501 tttctattta tttttttcta ttccgaatcc tctccttccc attttccaac aactgatgtt
     1561 gccaaagcat ttcctaaaac atttgttgca cttctgaaca tatcacaaaa atgatcaata
     1621 ggcaagatta aagcaatacc ttcaattgga attttaaaca ttccgcaagt agctgcaaca
     1681 atcactaaac tagctcttgg cactcctgct attcccttgc tagtaagcat caaaactaaa
     1741 agcattgtca tttgagttcc catatccaaa tcgattccat aggcttgagc gataaataaa
     1801 ctagcaaaag tcatatacat catacttcca tctaaattga aagaatagcc caaaggcaac
     1861 ataaacgcca ctattttatc ttttattcca aaacgttcca attcttcagt tagttttgga
     1921 aaaacagctt cgctactggt cgtactaaag gcaatgatta acggaccaac aatgtgattc
     1981 aataatgttt tcattctttt gccaagaaat aggaaaccaa tagctagaag aaaaatccat
     2041 aaagatccta ttccaaccaa aaatgaccca aaaaatttga tgtaagtaaa caccaactca
     2101 tttaaatctt tgatagcaaa aactcccgca atagctccaa aaacacccat tggagcaaac
     2161 ttcattacat aattcaccat tttaagaacg atatgagata acacttctat aaaatgagta
     2221 actggtttgg cataagtacc aattgatgct gctgctaatc caaaaaagat agagaaaatt
     2281 acaatttgaa taatttcatt attcgccatt gcttcaacaa tgcttttggg aataatatgt
     2341 tctacaaaat tttgggtaga aaaacttttg gtatttcctg tcacttctgc agcagtagat
     2401 aaatcaatat tagacaaatt cagtccaaca ccaggttgca atacattgac ccaaaataat
     2461 cctattaaaa gcgaaataaa agaagcgcta aaaaaccaac ctaatgcttt tccgcccact
     2521 cttccaacgg tttttacatc tcctaattta gcaattccag ggactaatgt actaaagacc
     2581 aataaagcaa ttatcatttg caccaatcga ataaaaacag tggccaatat ttttatctta
     2641 tcactaaatt ctttggcaaa agttgcatca taattatgat gaacaaaaat tcccaaaaat
     2701 gctcctaaaa gcattgcaat aacaatttga acagtaagat ttcctaaaaa ggatgttttt
     2761 ttggtttcag tcattttttt ttgggtattc agcatagaga gcgttctgtt tgcagttcga
     2821 actgaaacta caaattataa acccagaata ctatttaaag taagttttat ttatcaaata
     2881 aaaatctgcc agtactagag ctgccatcgc ttcaacaatg ggcactgctc gaggaacaac
     2941 gcaaggatcg tgacgccctt ttcctgtcat ttcggtaata tttccttgat tgtctaagga
     3001 ttcttgcttt tgcataatgg tcgccacagg cttgaaagca actctaaaat aaatatccat
     3061 tccgttgcta attccacctt gaattccgcc cgaaagattg gttctagtag ttccgtctgg
     3121 attgtataaa tcattgtgtt cgcttccttt catttttgcg ccacaaaaac cacttccaaa
     3181 ttcaaatcct tttacagcat ttatagaaag catcgctttg cctaattcgg cgtgtaattt
     3241 gtcaaaaacc ggttcgccca atccaattgg aacattttga atcacacaag ttacagttcc
     3301 gccaacagta tcaccatctt ttttgattac tttgatgtat tcttccattt ctgcagccat
     3361 cttttcatct ggacaacgca ctggattgct ttcaattttt gaaaaatcta attcttgata
     3421 aggtttttct aaaaataatg ggccaacaga agaaacgtaa gcattgattt ttatttcagg
     3481 aagaacttgt tttgcaatgg ctcctgccac tactctactg gctgtttcac gagctgaact
     3541 tcgtccgcca ccgcgataat ctcgaacgcc atattttttt tcgtacacat aatcggcatg
     3601 actaggtctg taattgtctt ttatgtgtga ataatcgtcc gacttttgat tggtatttgg
     3661 aatgataaac ccgattggag ttccggtagt tttaccttca aaaattccag ataaaaattg
     3721 aacatcgtct ggttcttttc gttgggtcac aattttagat tgtccgggtt ttctgcgtga
     3781 catttcgagc tgaatagcat caaaatctaa tgcgattcca gatggacatc cgtcaattat
     3841 tccgcctaaa gcttctccgt gtgattctcc aaaagtggtt attctaaata gggttccgta
     3901 gctatttcct gccattataa ttagttttga acaaatataa tattttagtt caaagttcaa
     3961 aagttaaaag tacgaagatt tgaatatcat ttcataatca actgattatt aattcttttt
     4021 ttatcttttg taaattgaaa ttaattttta tataaaattt tactcaaaaa aattatacct
     4081 ttgttttatg aaaaaaataa tcttcaccga aaaatcacca gcgcctattg gaccatacaa
     4141 tcaggctgtt cttaaaggaa atactcttta cacttcaggt caaattgcta taaatccagc
     4201 aactggcgaa ttggtcgttt ccgatatcga aagcgaaacc aaacaagtta tggaaaacat
     4261 gaaagccgtt cttgaagcag ctggaatgac attcgaaaat gttgtaaaat caactatatt
     4321 cattatgaat atgaatgatt ttgggactat caatagcgtt tatggttctt attttgacga
     4381 aaaaaccgct ccagctcgtg aaacggttca agtggcgggt ttgccaaaaa atgtaaatgt
     4441 cgaaatttct atgattgcta ttttatagct atagcatcta tgaataattg agccgaagct
     4501 tcatttgtag ctaatggaac gttgtgcaca tcgcaaacac gcaacaacat attcacatcc
     4561 gattcgtgtg cgtgacttga taaagggtct ttgaaaaaca agaccatttt agtttttcct
     4621 tcagcaactc ttcctgcaat ttgagcatcg cctccttgtg gaccagaaag catttttttt
     4681 actttaaagc cagcactttc ggcctttgat cctgttgtgc cagtaccaat aagttttata
     4741 tcttctcgtt gcaaaaccgt tttgtttttg tttaaaaact gaaccatatc ggctttttta
     4801 ccgtcgtgag caataattgc tatttccatt ttcaaattaa tataaaatct gttaaagaat
     4861 tatttgttgg caacatgata tgcaataagg ccatctattg gtcgtctcaa tacatttcct
     4921 aatttcattt catatttatc aaacataact tgcagttctt cttttagata aaatgctatc
     4981 gaacctacga aatgtacagg aacttcttta caattatcat attgttgaac atagtttttg
     5041 atgaaagatt tcatcccttt gaaaatgatt ttttgacaaa atggatgctc tttattttgg
     5101 ataagaaact ttgcaaatgt ggctaaatag gcatttggat taggttcttt gtataattta
     5161 tttttaatga aatcaggatc taaatcatac tctttttcaa attctactgc caattcttta
     5221 ggcattttat tgaaataata ttttctgatt aattctttac caagcacatt accactacaa
     5281 tcatccatca cgatgtatcc taatgattgc actttttgat gcaaaacttt cccgtcaaaa
     5341 taactacagt ttgaccctgt tccaagaatg ctaacaatgg cttcttctcc ttttggggtt
     5401 gtagcataca ccgcagcata agtatcttct tgaacgtcaa caattgcatt tggaaaatag
     5461 tcttgaaaaa ttctagaaag atcgattttc attctatcgg ttccacatcc tgcaccataa
     5521 aagaatagat gtgttgcttt ttctttattt tgcaaaatat caaaacgatc gtttaatctt
     5581 tcgactattt catttccatc aagaatttca ggatttaagc ctaatgtttg tgtggtgaaa
     5641 agtatttttc catcttcatc aattgcaatc caatcggctt ttgtagagcc gctatccact
     5701 aataatctca ttgagtttca agttttaagt ttcaggtttt aggtggacta aaaactaaaa
     5761 aaaattaata aaaaaatccc gctaaaataa atctaacggg attttgcttt gtaaatgtaa
     5821 ttattttaag cttgtgatat gcacagcaag gtcaattaat ttacttgaat acccatactc
     5881 attatcatac caagatacta gtttgaagaa agttgaattc aaaccgattc ctgcagtagc
     5941 atcaatgatt gaagttcttt tatcagaaat aaaatcttga gaaaccacag catcttctgt
     6001 gaatcctaaa atacctttca atgaagtttc agaagcattt ttcaatacag ccataatttc
     6061 ttcgtatgaa gtttctttag caacttttac agtcaaatct actacagaaa cgtcagcagt
     6121 aggaacacgg aaagacattc ctgttaattt tccattcaaa gaaggaatta cttttccaac
     6181 cgcttttgca gctccagtcg aagaaggaat aatattaatt gcagcagcac gtccacctct
     6241 ccagtctttt cttgaagga
//
LOCUS       DAOSNN010000158         6215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12709_length_6215_cov_4.483117, whole genome shotgun sequence.
ACCESSION   DAOSNN010000158 DAOSNN010000000
VERSION     DAOSNN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12709_length_6215_cov_4.483117"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..306)
                     /locus_tag="PLQ70_08530"
     CDS             complement(19..306)
                     /locus_tag="PLQ70_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34697.1"
                     /translation="MKKSVPKSELEIILHKKGTEIIKNKIPIDDSNHITSEIIKILNE
                     FEILSSLESKKLKEELKITQEYSEITGPKYTELYDFAPSGYYILSDSAEIL"
     gene            complement(708..4934)
                     /locus_tag="PLQ70_08535"
     CDS             complement(708..4934)
                     /locus_tag="PLQ70_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQE34698.1"
                     /translation="MKTENIAKESITANEDKYRALFESNRDSVTIFRLDSKGNSGHFI
                     EVNLATTELFGFSQKELLAMRFNDLEIISEKEKIYRIKQLLSKGKIDFETIIRDKNGD
                     NRNIEVKAVLINYEGSPAVMNITRDISVSKKIEENSRKTQENLKTILEAIPDLLFEVG
                     YNGKIYNYHSHNKDFLAVPPDEIVGSFFQNTLPQEVVATCFSAIEEANENGWSLGKQY
                     SLQLPQGKIWFELSVSKTKETKEKNIDNRFIILARNITERKAIEKALIENEKFLKQTQ
                     LIAQLGFYTLEIETGKWISSELLDSIFGIDNNFNRTIESWASIIHPQWQQTMIDYFAN
                     EVIGKKENFNKEYQIIRQNDKEVRWVWGNGELIFNEKNEPIKMIGTIQDITERKLAEF
                     EIIKSKEEAEKSREYLDKIINNVGDPLFVKDEQSRILIANDAFCKLFGINKIDIIGKT
                     LAENVSEEEQESFLKIDKQVIADGLENINEETLTIRGGESLTISTRKTRFIDANGKKL
                     IVAVIHDISERKKTEIALRKSEENLQSIFDSVSEAIYVLDENGTFIDVNKGAELMYQL
                     SKSELIGKNPQSVAAPGLNDLNEVNRIIQSVFDNGVPISLEFWSIRKNGEIFPKEVVV
                     NRGKYYGKYVLIVTARDITKRKRNEIALRKSEEKLMNIFNLANSGIILADLNGNFLEF
                     NNWWLKMTGYSQEEFSNLKNLGVTHPDDLEKSKRMLSKLINGEIDKYELEKRYVKKDK
                     TIFWGQTSVSAIKDKNNTITSTIGVVTNITERKLAEELLRESEKKYRGLVENSPDGII
                     TYVNDKITYANDEAIRIVNADNSDQIIGKSVLEFVHQDSLESVIEKMKEIIVDEYASR
                     VVEEKLVSLTGQTVYVELKAIPTLYEKQNAVQVIIHDITEQKQTQDKIKQLSQAVEQS
                     PVAIEITDKFGTIEYVNSKFLETTGYSYDEIIGVNSNILKSGHTSPTEYKKLWDTINS
                     GKEWYGEFHNKKKDGTKYWEFASISPILNSQGITTHYIAIKEDITERKIIEQQLILAK
                     EKAEESDRLKLAFLANMSHEIRTPMNGILGFTELLKEKDLSSDIQQEYINIIEKSGKR
                     MLNIINDIISISKIESGQIEITTSETNINELFDYLTTFFKPEAKHKQISLFSSKALPP
                     NESFIKTDREKLYAILTNLVKNAIKFTSNGSIELGCEKKEGYLEFFVKDSGLGISDSQ
                     KNIIFERFRQANDTISRTHEGSGLGLAISKAYVEMQGGHIWVESKEGVGSSFYFTLPF
                     YSEYESKENLVVEKITSCDQEENNIKDLKVLIVEDDAISKLLITIAVKPFSKETLKVS
                     TGYDAIETCRNHPDIDLVMMDINMPEMGGYEATKEIRKFNKDLVIIAQTANGMQSDHD
                     KAIAAGFTDYISKPINISTLSKLICKHFKK"
     gene            complement(5046..5522)
                     /locus_tag="PLQ70_08540"
     CDS             complement(5046..5522)
                     /locus_tag="PLQ70_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQE34699.1"
                     /translation="MTANEGIIIKFYTAFANADFNQMCECYHPKIQFSDPAFGTLNGN
                     DVCQMWKMLLEKSKGNIKIEFSEIKTKGQYGSALWIATYNFSKTNHKVVNSVSAKFHF
                     QDGLIVKHIDDFDIWKWAKQALGIKGMLLGWTGFMQKKIQENARKTLKTYCEKNNI"
     gene            complement(5667..>6215)
                     /locus_tag="PLQ70_08545"
     CDS             complement(5667..>6215)
                     /locus_tag="PLQ70_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQE34700.1"
                     /translation="KNPNDLEVYIETYLKKNNAKVAFGGYNEVRNLYKQSSLFGDGQA
                     DKRNIHIGMDLWIKAGTPVLAALDGIVYGFDFNAGKGNYGPTIILRHNLGNHFFYTLY
                     GHLSIESIQNIEIGSFFKKGQQLAILGDSTVNGGYSPHLHFQIIKNIEDNFSDYPGVC
                     NQKDLEYYLENCPDPDLLLKIK"
BASE COUNT         1790 a         1000 c          921 g         2504 t
ORIGIN      
        1 ggctcccatt aagtttaatc atagaatttc ggctgaatca gatagaatgt agtagcctga
       61 aggcgcaaaa tcatataatt cagtatattt tggaccagta atttctgaat attcttgggt
      121 tatttttaat tcttctttta gttttttgct ttctaatgaa ctaagaattt cgaattcgtt
      181 taaaatctta attatttctg atgttatatg gttggaatca tcaataggta ttttgttttt
      241 tataatttca gttccttttt tatgaagaat tatttcaagt tctgattttg gaacgctctt
      301 tttcataatt attgagtgtt tgaattatga tttaattttg ctttttttca aagttttagt
      361 actttgaaat agtaccagat ttttttttga aataagtcaa cttttttatt caatgattga
      421 agtattgatt ttattgtaat ccatcattat tttaaagagg aaagtctgga tgtttaatta
      481 gctatttaat gaatttaact taagaagttt cttaatagct attagtaaat aagcaaccta
      541 tatacactca aatttagtaa aaaaatatca tatttttata taaaacccac tatttttcta
      601 tagagtaaca gaaatgatag tttttataaa ctattgatta gtagttaata agaaataaaa
      661 ataggaaaaa atataaatat tgatgtatta aaatatatta ttcgaattta tttcttgaaa
      721 tgcttgcata ttaatttgct taatgtagaa atgtttattg gttttgaaat gtaatcagta
      781 aatccagctg caatggcttt gtcatgatcg ctttgcattc cgtttgcggt ttgagcaatg
      841 attactagat ctttattgaa tttacgtatt tctttcgtgg cttcataacc acccatttcg
      901 ggcatattta tatccatcat cactaagtct atatcaggat gattgcgaca ggtttcaatc
      961 gcgtcataac ctgtgctcac tttcaaagtt tctttgctaa aaggttttac agcaatagta
     1021 attagcaatt tagagatcgc atcgtcctcg actattaata ctttgaggtc tttgatgttg
     1081 ttttcctctt ggtcacaaga tgtaattttt tctacaacca agttttcttt actttcatat
     1141 tctgaataga aaggtagtgt aaaataaaaa ctgcttccaa ctccttcttt actttctacc
     1201 caaatgtgtc cgccttgcat ttcaacatag gcttttgaaa tggccaatcc taaacctgaa
     1261 ccttcgtgtg ttcgactaat ggtatcattg gcttgtctga acctttcgaa aattatattt
     1321 ttttgagaat ctgaaattcc taaaccagaa tctttcacga aaaattctag ataaccttct
     1381 tttttttcgc aacccaattc gatgctacca ttgcttgtaa atttgatggc atttttcaca
     1441 agattagtta gaattgcgta taatttttca cggtctgttt ttataaaact ttcatttgga
     1501 ggcaatgctt tagatgaaaa aagtgatatt tgtttgtgtt tggcttctgg tttaaagaaa
     1561 gtggttaaat agtcaaacag ttcatttata ttcgtttcgg atgttgttat ttctatttgt
     1621 cctgattcaa tttttgaaat actaatgatg tcattgatga tgtttagcat tcgtttgcca
     1681 cttttttcaa tgatgtttat atattcttgt tgaatatcac ttgacaaatc tttttctttt
     1741 aaaagttcgg taaaacctag aataccattc attggagtac gaatttcgtg actcatattg
     1801 gcaaggaaag ctaattttaa acgatcactt tcttctgctt tttctttagc taagatgagt
     1861 tgttgttcaa taattttgcg ttctgtaata tcttctttaa ttgcaatata atgggttgta
     1921 attccttgtg aatttaaaat tggagatatt gacgcaaatt cccaatactt ggttccgtct
     1981 tttttcttgt tatgaaattc gccataccat tctttgcctg aatttatggt gtcccataat
     2041 tttttatact cagtgggcga ggtatgacct gattttaaaa tattcgaatt taccccaata
     2101 atttcatcat aggaataacc ggttgtttca agaaattttg aattgacgta ttcgattgtt
     2161 ccaaatttat ctgtaatctc aatagcaaca gggctttgtt ctactgcttg cgatagttgt
     2221 tttattttat cttgtgtttg tttttgttca gtaatgtcgt gaataataac ttgaacagca
     2281 ttttgttttt catataatgt tgggatagct tttagttcga catatactgt ttgaccggtt
     2341 aaagaaacaa gtttttcttc gaccactcta gaagcatatt cgtcaacaat tatttctttc
     2401 attttttcaa ttacgctttc taggctatct tgatgaacga attctaagac cgattttcca
     2461 ataatttgat cactattgtc ggcatttaca attcgaatcg cttcatcatt ggcataagtg
     2521 attttgtcat tgacataggt aataattccg tctggtgaat tttcaaccag acctcgatat
     2581 tttttttcgc tttctcgtag caactcttcg gctaattttc tttcggttat gttagtaaca
     2641 actccaatag tgcttgtaat agtattgttt ttatctttaa tagcagacac agaggtttga
     2701 ccccaaaaga ttgttttgtc tttttttaca tagcgttttt caagttcata tttatctatt
     2761 tcgccattta ttaattttga taacatcctt ttgcttttct ccaaatcatc tggatgggta
     2821 acacccaaat ttttcaagtt gctgaattct tcctgcgaat agcccgtcat tttgagccac
     2881 caattgttga attccaaaaa attaccattc aaatcggcta gaataatccc tgaattggct
     2941 agattaaaaa tattcattaa cttttcttcg ctttttcgaa gagcaatttc gttccttttg
     3001 cgtttggtta tgtcgcgggc ggtaacgatt agaacgtatt ttccataata ttttcctcta
     3061 ttgacgacca cttctttggg gaaaatttca ccatttttgc gaatagacca gaattctagc
     3121 gagataggaa caccgttgtc aaatacagat tgaattattc tatttacttc gtttaaatca
     3181 ttgagtccag gggcagcaac gctttgaggg ttttttccaa tgagttctga tttagacaat
     3241 tgatacatta attcggcacc tttatttaca tcaataaaag ttccattttc atcaagtaca
     3301 taaatggctt ccgaaactga atcaaaaata gattgcaggt tttcttcact ttttctgagg
     3361 gctatttcag tttttttacg ttcagaaata tcgtggatta ctgccacaat caactttttg
     3421 ccatttgcat ctataaatcg ggtctttctt gttgaaattg ttaaagactc accacctctt
     3481 attgtaagcg tttcctcgtt tatattttct agaccatctg caattacttg tttatctatt
     3541 tttagaaaag attcttgttc ctcttcactt acattttctg caagtgtttt gcctataatg
     3601 tctatcttgt ttataccaaa taatttacaa aatgcgtcat ttgcaatcag aatcctactt
     3661 tgttcatcct ttacaaaaag aggatcacca acattgttaa tgattttatc taagtattct
     3721 ctgctttttt ctgcttcttc tttggatttt attatttcaa attctgcaag tttgcgttca
     3781 gtaatgtctt gaattgtacc tatcattttt ataggctcat tcttttcatt gaaaattagt
     3841 tctccatttc cccaaaccca tcgcacttct ttatcatttt gtctaatgat ttggtattct
     3901 ttattaaaat tttctttttt gccaatcact tcattggcaa aatagtctat catcgtttgt
     3961 tgccattgag gatgaataat ggatgcccaa ctttcaattg tacgattaaa gttgttgtct
     4021 atgccaaaaa tcgaatcaag aagttcagaa cttatccatt tgccagtttc aatttctaaa
     4081 gtataaaatc ctagctgtgc tataagttga gtttgtttta aaaatttttc gttttctata
     4141 agtgcttttt ctattgcttt tcgctcagtg atgtttcgtg cgaggataat gaagcgatta
     4201 tctatgtttt tttctttggt ttcctttgtt tttgagaccg aaagctcaaa ccaaatcttt
     4261 ccttgcggca attgcagtga atattgtttg cctaaagacc atccattttc gttagcttct
     4321 tcaattgctg aaaagcaagt tgctactact tcttgaggta aagtgttttg aaaaaaactt
     4381 ccaacaattt cgtcgggtgg aacggccaaa aaatctttat tatgtgaatg gtagttatat
     4441 attttgccat tataaccaac ttcaaaaagt aaatctggta tagcttcgag aattgttttt
     4501 aggttttctt gagtctttct tgagttttct tcaatttttt tgctaacaga aatatctcgt
     4561 gtaatgttca ttacagcagg actaccttca taatttatta aaaccgcttt tacctcaata
     4621 tttctgttgt caccgttttt gtctctaata atggtttcaa aatctatttt tcctttagac
     4681 aataattgtt ttatcctgta gattttttct ttttcggaga ttatttcaag gtcattaaaa
     4741 cgcatcgcta ataactcttt ttgactaaat ccaaaaagtt cagttgtagc taggttgact
     4801 tcaataaaat gacctgaatt ccctttcgag tcaagtctga atatagtaac actatctcta
     4861 tttgattcga aaagggcacg atacttatct tcgtttgcag tgatgctttc ttttgctata
     4921 ttttctgttt tcatataaga atgaagattc tttaatgttt cagtttttag gattgttttg
     4981 aattaatttc aaaacattaa ttggtgtaaa aaataggttt tactaaaaaa aagaataatt
     5041 atgattcaga tattattctt ttcacaatat gtttttagcg tttttcgagc gttttcttga
     5101 atttttttct gcataaatcc tgtccatcct agaagcatac ctttaattcc caatgcttgt
     5161 tttgcccatt tccaaatgtc aaaatcatcg atgtgtttga caatcaatcc gtcttgaaag
     5221 tgaaattttg ccgatactga atttaccact ttatgatttg ttttgctaaa attgtaagtg
     5281 gcaatccaaa gtgcagagcc atactgtcct tttgttttaa tttctgagaa ttcgattttt
     5341 atgtttcctt tgcttttttc taggagcatt ttccacattt gacatacatc attgccgttt
     5401 aatgttccaa aagcaggatc actaaattgt attttgggat ggtaacattc acacatttga
     5461 ttgaaatcag catttgcaaa tgcggtgtag aattttatga tgatgccttc gtttgctgtc
     5521 attaatttaa ttgtataata aacgtaaaac taatgatttt tttttagaat tactttttta
     5581 tagcatctaa tttagtaata aaacagggat aaactcaaaa taaaaaaaat gtaattttta
     5641 atgtagaaat tgctattaaa tatttattat ttaattttta ataataaatc ggggtcagga
     5701 caattttcta ggtaatattc caaatctttt tggttgcaaa caccaggata atcactaaaa
     5761 ttatcttcta tgttttttat aatttgaaaa tgcaaatgcg gcgaataacc accatttact
     5821 gtcgaatctc ccaaaatagc tagctgttgt ccttttttga aaaatgagcc aatttcaata
     5881 ttttgaatac tttcaattga taaatgaccg tataaagtat aaaaaaaatg atttcctaaa
     5941 ttatgtctta agataatggt tggtccataa tttccctttc cagcgttgaa atcaaatcca
     6001 taaacgattc cgtcaagtgc tgctaatact ggcgttccag cttttatcca caaatccatt
     6061 ccaatatgaa tgtttctttt atctgcttgt ccatctccaa aaagtgacga ttgtttgtaa
     6121 agattcctca cttcattata acctccaaaa gctacttttg cattgttttt tttaagataa
     6181 gtttcaatat aaacttctaa atcattgggg ttttt
//
LOCUS       DAOSNN010000159         6210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12720_length_6210_cov_4.401300, whole genome shotgun sequence.
ACCESSION   DAOSNN010000159 DAOSNN010000000
VERSION     DAOSNN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12720_length_6210_cov_4.401300"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLQ70_08550"
     CDS             <1..659
                     /locus_tag="PLQ70_08550"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006264317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HQE34701.1"
                     /translation="DVHMTDLISGRETLEDIQFIGAVGGFSNSDVLGSAKGWAGAFLY
                     NEKAKNALDNFFKREDTLSVGICNGCQLLMELEKINPEHEVHGKMLHNDSHKHESIFT
                     SVTIQENKSVMLSTLVGSTLGVWVSHGEGKFNLPMGEENYNIVGKYGYDSYPANPNGS
                     DFNTAMLCDTTGRHLVMMPHIERSTFQWNWANYPKDRNDEVTPWHEAFVNARLWIEKH
                     "
     gene            776..2548
                     /locus_tag="PLQ70_08555"
     CDS             776..2548
                     /locus_tag="PLQ70_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQE34702.1"
                     /translation="MKKITRLFDFPYYQLEHYNISDALVTKYNGVWVKTSTQEYIAKA
                     NTISRALLQMGIQKNDKIALISSNNRTEWNIMDIGILQTGAQNVPIYPTISETDYEYI
                     LNHSGSIYCFVSDIEVLEKVNSILPNLPNLKGVYSFDEIVGCKNWKELLTLGEDQSNQ
                     EAVEERKNSIQTDDLATIIYTSGTTGKPKGVMLSHKNIVSNVLDSASRIPFTAGQSRA
                     MSFLPICHIFERMVLYIYQFYGVSVYFGESIDKISDNIKEVRPTVMTAVPRLIEKVYD
                     KIYSKGLELKGIKRKLFFWAVDLGLKYEPYGANGFWYEFQLKIARKLIFSKWKEGLGG
                     NLDLLVSGSAALQPRLARVFAAAEMPVVEGYGLTETSPVIAVNDMRNFGFKVGTVGKL
                     IDNVEVKIAEDGEILCKGPNVMLGYYKDEELTKKALVDGYFHTGDIGEIDSSGFLKIT
                     DRKKEMFKTSGGKYISPQLLENAMKQSRFIEQIMVIGDGQKMPAAFIQPNFEFVKTWE
                     KLHGIQVGTTNEEIISNPKVIERIQEEVDILNEKFGNWEKIKRFELTPDVWSIDGGHL
                     TPTLKLRRKIVMEKYIDLYNKIYN"
     gene            2729..3160
                     /locus_tag="PLQ70_08560"
     CDS             2729..3160
                     /locus_tag="PLQ70_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQE34703.1"
                     /translation="MKDKTIDYVLRATWQAVSRMYNEEATKYGATMATGFALLSIDKE
                     EGTPSTALGPKMGMEATSLTRTLKNMEEKGLIYKKPNPVDGRGVLIYLTEFGKEKREL
                     SKETVLKFNEAVKQNVSAEKLQHFMEVSEIINDLITEKKIF"
     gene            3236..5626
                     /locus_tag="PLQ70_08565"
     CDS             3236..5626
                     /locus_tag="PLQ70_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HQE34704.1"
                     /translation="MKRSIKKVAVIGSGIMGSGIACHFANIGVEVLLLDIIPNELTEA
                     EIKKGLTLENKIVRNRLVNEHLQNALKSKPSPIYHQKFASRITTGNTTDDMAKIANVD
                     WIIEVVVERLDIKKLVFEQIEKFRKPGTLITSNTSGIPIHFMSEGRSEDFQKHFCGTH
                     FFNPARYLKLFEIIPGPKTSVEVLDFLTTYGEKFLGKTSVVAKDTPAFIGNRIGIFGI
                     QSLFHLVKEMGLTIEEVDKLTGPVIGRPKSATFRTVDVVGLDTLIHVANGIYENCPND
                     EAHELFKLPDFITKMMENKWLGSKTGQGFYKKVGSDILSLDLNTLEYRPAKKASFATL
                     EMTKTIDKPINRFKVLVKGTDKAGEFYRKSFAGLFAYVSNRVPEISDELYKIDDAMKA
                     GFGWENGPFEIWDAIGVANGIEIMKAEGLVPATWVSDMLASGTSSFYSIKEGATYFYN
                     IPNKTQTKVPGQDAFIILNNIRESKKVWSNSGAIIQDLGDGILNLEFQSKMNTIGGDV
                     LQAINKAIDLSEKEYQGLVIGNQGANFSVGANIGMIFMMAVEQEYDELNMAIKMFQDT
                     MMRVRYSGIPVVVAPHGMTLGGGCEMSMHADKVVAAAETYIGLVEFGVGVIPGGGGSK
                     EMTLRASDLFRKNDVELNVLQEYFLTVAMAKVSTSAYEAFDLGVLQHGKDIVIVNKDR
                     QIAEAKKHALLLAEAGYTQPIRRNDIKVLGKQALGMFLVGTDQMQAGKYISEHDKKIA
                     NKLAYVMAGGDLSEPTLVSEQYLLDIEREAFLSLCTERKTLERIQYMLTKGKPLRN"
     gene            5701..6057
                     /locus_tag="PLQ70_08570"
     CDS             5701..6057
                     /locus_tag="PLQ70_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQE34705.1"
                     /translation="MHNFEKLKIWQKAMDIAVAVYDVSLQLPSDEKFNLIHQIKKCAV
                     SIPSNIAEGSGRNHNKEFIQFLGIANGSTFELITQLILAKRLNLIKEETIQPIISQLV
                     EVSNMNFAFQKTLKTT"
BASE COUNT         2200 a          977 c         1213 g         1820 t
ORIGIN      
        1 aagacgttca tatgaccgac ttgatttctg gacgtgaaac attggaagac attcagttta
       61 ttggagccgt tggaggattc tcgaattctg atgttttggg ttctgccaaa ggttgggctg
      121 gagccttttt atacaatgaa aaagccaaaa acgctttgga caatttcttc aaaagagaag
      181 atactttgtc agtcggaatc tgtaacggtt gtcagttgtt gatggaattg gaaaaaatta
      241 atccagagca cgaagttcac ggaaaaatgc ttcataatga cagtcacaaa cacgaaagta
      301 tttttacctc ggtaacgatt caggaaaaca aatcggtgat gttatcgact ttggtgggaa
      361 gcactcttgg agtttgggtt tcgcacggtg aaggaaaatt caatttgcca atgggtgaag
      421 aaaactacaa catcgttgga aaatacggtt acgacagtta tcctgccaat ccaaatggtt
      481 ctgattttaa taccgcaatg ctttgcgaca caacaggtcg tcatttggta atgatgccac
      541 atatcgagcg ttctactttc caatggaact gggcgaatta tccaaaagat agaaacgacg
      601 aggttacgcc ttggcacgaa gcctttgtaa atgccagatt gtggattgaa aagcattaat
      661 ctaaaaataa atactattcc attaaggagt tgattttttt cgactccttt ttttttgtta
      721 aaaagacaaa aacatcatat tttttactaa tttgcaatat taaacactat tccaaatgaa
      781 aaaaatcacc cgattatttg attttcctta ctaccaatta gagcattata acatttcgga
      841 tgcgttggtc accaaatata atggggtttg ggtaaaaact tcgacccaag aatacatcgc
      901 aaaagccaat acaatttcta gagctttatt gcaaatggga attcaaaaaa acgataaaat
      961 tgccttaatt tcatcgaata atagaaccga atggaatatt atggacattg gaatattgca
     1021 aactggggca caaaatgttc ccatttatcc aactatatct gaaacagatt acgaatatat
     1081 actaaatcat tccggttcga tatattgttt tgtatcggat attgaagtgt tagaaaaagt
     1141 aaattccatt ttacctaatc ttcctaatct aaaaggagtt tattcttttg atgaaattgt
     1201 aggctgcaaa aactggaaag aactactaac tcttggcgaa gaccaaagca accaagaagc
     1261 tgtcgaagaa cgaaaaaata gcatacaaac cgatgattta gccactataa tttatacttc
     1321 tggaactaca ggcaaaccaa aaggtgtaat gctttcgcat aaaaacatcg tttctaatgt
     1381 gttggatagc gcttcgagaa ttccattcac agctggccaa agtagagcca tgagcttctt
     1441 accgatttgc catattttcg aaagaatggt tttatatatc tatcagtttt acggtgtttc
     1501 ggtatatttt ggcgaatcga ttgataaaat tagtgataac atcaaagaag taagaccaac
     1561 cgtaatgacg gctgtaccaa gacttattga aaaagtatat gacaaaatct actctaaagg
     1621 attagagtta aaaggaataa aaagaaaatt atttttttgg gctgtagatt tgggattaaa
     1681 atatgaacct tacggagcta atggtttttg gtatgaattt caattaaaaa tagcccgaaa
     1741 actcattttc agcaaatgga aagaaggtct gggaggcaat ttagatttat tagtttcagg
     1801 aagtgctgct cttcaaccaa gattagccag agtatttgcc gcagccgaaa tgcctgtagt
     1861 agaagggtat ggattaaccg aaacttctcc tgtgattgct gtgaacgata tgcgaaattt
     1921 tggtttcaag gtgggaactg ttggaaaatt aatcgataat gtagaagtca aaattgctga
     1981 agacggagaa attctttgca aaggtcccaa tgtaatgctg ggttattaca aggacgaaga
     2041 attaaccaaa aaagccttag ttgatggcta tttccacact ggcgatattg gcgaaattga
     2101 tagctcaggt tttcttaaaa ttaccgatcg aaagaaagaa atgtttaaaa cttcgggtgg
     2161 aaaatacatt tcgccacaat tacttgaaaa cgctatgaaa caatcccgtt ttatagagca
     2221 aattatggtc attggcgatg ggcaaaaaat gccagctgct ttcattcagc ccaattttga
     2281 atttgtaaag acttgggaaa aactacacgg aattcaagtg ggtacgacca acgaagaaat
     2341 tatttcaaac ccaaaagtaa ttgaacgcat tcaggaagaa gttgatattt tgaatgaaaa
     2401 atttggaaat tgggaaaaaa taaaacgttt cgaattaact ccagatgttt ggtctattga
     2461 cggagggcac ctcactccta ctctaaaact tagacggaaa attgtaatgg aaaagtatat
     2521 tgatttatat aataaaatat acaattagca ttgtatgtat tatcatttat gatttttatt
     2581 ttttcaaaca attaatcatt atttaaatac tcaaactctt caatttttga agagtttttt
     2641 ataccaaatt aacaaatttt aaaaaattaa catatttatt atgcgtgcat agtatttttt
     2701 ttattacatt tgaaaattcc aaaaaaatat gaaagataaa accatagatt atgttcttcg
     2761 tgccacttgg caagctgtgt caagaatgta caacgaagag gctacaaaat acggtgctac
     2821 aatggcaact ggttttgcct tgttgagtat tgacaaagag gaaggaacac catcaactgc
     2881 cttggggcca aagatgggaa tggaagctac gagcctaacc agaacattaa aaaacatgga
     2941 agaaaaagga ctgatttaca aaaaacccaa ccctgttgac ggtcgaggtg tattgattta
     3001 tctaaccgaa tttggaaaag aaaaaagaga attatcaaag gaaaccgtgt tgaaatttaa
     3061 tgaagcggta aaacaaaatg tatctgctga aaaattgcag cattttatgg aagtttcaga
     3121 aattataaat gacttaatca ccgaaaagaa aatattttaa gtgtattaac gcttgtccta
     3181 tttaaaagtc aattgcaaga atcacaacaa tcaaaaaaaa cataaaccgt ttaaaatgaa
     3241 acgatctatc aaaaaagttg cagtaattgg atccggaatt atgggttcgg gaattgcttg
     3301 tcattttgcc aatattggcg tagaagtatt gcttttggat ataattccaa acgaactcac
     3361 cgaagccgaa atcaaaaaag ggttaacttt agaaaacaaa atagttcgca atcgtttggt
     3421 gaatgaacat ctccaaaatg ctttaaaatc aaagccatcg cctatttatc atcaaaaatt
     3481 tgctagccga atcactactg gaaatactac cgacgatatg gcaaaaattg ctaatgtcga
     3541 ttggattatc gaagtggttg ttgaacgttt ggatatcaaa aaattagttt tcgaacaaat
     3601 agaaaaattc cgcaaaccag gaacattaat tacttctaat acttctggaa ttccgattca
     3661 ttttatgagt gaaggaagaa gcgaagattt ccagaaacat ttctgcggaa cgcacttttt
     3721 taatccagct cgttatttaa aattattcga aatcattcca ggaccaaaaa cttctgttga
     3781 agtattggat ttcttaacga cttatggcga aaaattctta ggcaaaactt cggttgtggc
     3841 caaagatact ccggcattta ttggaaacag aatcgggatt ttcggaatcc aaagtttgtt
     3901 tcacttggtc aaagaaatgg gattgaccat cgaagaagtc gataaattga ctggtccagt
     3961 tattgggaga ccaaaatcag ccactttccg tacggttgac gtggttggtt tagatacttt
     4021 gatacacgtt gccaacggaa tttatgaaaa ctgcccgaac gacgaagctc acgaattgtt
     4081 taaactgcct gatttcatca caaaaatgat ggaaaacaaa tggttgggta gcaaaacggg
     4141 gcaaggtttt tataagaaag taggttctga tattctttct ttagatttaa atactttaga
     4201 atatcgccct gccaaaaaag cttcttttgc tactttagaa atgactaaaa ccattgacaa
     4261 acctatcaat cggtttaaag ttttggtaaa aggaacagac aaagcaggtg aattttacag
     4321 aaaaagtttc gctggattat ttgcttatgt ttccaataga gttcctgaaa tttcagatga
     4381 attatacaaa atcgatgatg ctatgaaggc gggttttggt tgggaaaacg gtccgtttga
     4441 aatttgggat gccattggtg tggccaacgg aatcgaaata atgaaagccg aagggttagt
     4501 tccagcaact tgggtttctg atatgttagc ttctggaact tcaagttttt attctataaa
     4561 agagggagct acttattttt ataatatacc aaacaaaaca caaactaaag ttcctggaca
     4621 agatgccttc atcattctga acaacattcg cgaaagcaaa aaagtttgga gtaatagtgg
     4681 agccatcata caagatttag gcgatggaat tttgaattta gagtttcaat cgaaaatgaa
     4741 taccattggt ggcgatgttt tgcaagctat caataaagcc attgatttat ccgaaaaaga
     4801 ataccaaggt ttggttattg gaaatcaagg cgctaatttt tctgtgggtg ctaatatcgg
     4861 aatgatattt atgatggcgg tcgaacaaga atacgacgaa ttgaatatgg ccatcaagat
     4921 gttccaagat acgatgatgc gggtgcgtta ttcaggaatt ccagttgtgg ttgctcctca
     4981 cggtatgact ttgggtggtg gttgcgaaat gagtatgcac gctgataaag tcgttgctgc
     5041 tgcagaaact tatatcgggt tggtggagtt tggtgttggc gtaattcctg gtggcggcgg
     5101 atcaaaagaa atgaccttga gagcatcgga tttattccgc aaaaatgatg tggaattgaa
     5161 tgtgttgcaa gaatatttct tgaccgtggc aatggcaaaa gtttcgactt cggcttatga
     5221 agcctttgat ttaggcgttt tgcaacacgg aaaagacatt gtcattgtca ataaagatcg
     5281 ccaaattgca gaagccaaaa aacacgcatt gctactagcc gaagcaggtt acacccaacc
     5341 gattcgcaga aatgacatta aagttttagg aaagcaagct ttagggatgt tccttgttgg
     5401 aactgaccaa atgcaagccg gaaaatacat ctccgaacac gacaagaaaa tcgccaacaa
     5461 actagcctat gtaatggctg gaggcgattt atccgaacca acattagtaa gcgaacaata
     5521 tttattggat attgaaagag aggctttttt gagtttgtgt acagaaagaa agacattgga
     5581 gagaattcag tatatgttaa ccaaagggaa accgttgagg aattagtatt aagactggag
     5641 tattaagtat taagactgaa agatttagtt ttaagactta gctaataaaa aatatagact
     5701 atgcacaact ttgaaaaact taaaatatgg cagaaagcaa tggatattgc tgttgccgtg
     5761 tatgatgttt ctttacaatt gcctagtgat gaaaagttta atttaattca tcaaattaag
     5821 aaatgcgcag tttcaattcc ttcaaatatt gcagaaggtt caggaagaaa tcataacaaa
     5881 gaatttattc agtttttagg aattgcaaat ggatcaactt ttgaattaat aacacagtta
     5941 atactagcaa aaagattaaa tcttataaaa gaagaaacaa tacaacccat tattagtcaa
     6001 ctagtagaag tttcaaatat gaatttcgct tttcaaaaaa cattaaaaac aacataaaaa
     6061 acaaagatta aaagtcttaa tacttactac taaaatctta atgcaaaaca gataaagtct
     6121 taatacttaa tacttaaatc ttaatacttt tcaaatatga aaacagccta tatagtaaaa
     6181 gcctaccgaa cagccgttgg aaaagcccca
//
LOCUS       DAOSNN010000160         6192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12762_length_6192_cov_4.596383, whole genome shotgun sequence.
ACCESSION   DAOSNN010000160 DAOSNN010000000
VERSION     DAOSNN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12762_length_6192_cov_4.596383"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..854)
                     /locus_tag="PLQ70_08575"
     CDS             complement(21..854)
                     /locus_tag="PLQ70_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HQE34706.1"
                     /translation="MKIKFLFIAVILTAIFCAFSFSKTNNSVKSNLYGNTIQHRFQPP
                     AGFVRQAESESSFGFFLRDLPLKPSGSNVLYFDGTVKSNRNVYEAVVNLPIGKQDLHQ
                     CADAVMRLRADYFYSLKQYDKIHFNFTNGFRVDFSKWAAGYRIAVKGNKTSWVKTAKP
                     SVSYETYWNYLETVFMYAGTASLEKELKTIKVSDVKIGDVFIKGGFPGHAVIVVDVAV
                     NPKTNQKVMLLAQSYMPAQELQILKNPNNVSINPWYDTDFGSTLKTPEWTFNASQLKR
                     F"
     gene            complement(911..2938)
                     /locus_tag="PLQ70_08580"
     CDS             complement(911..2938)
                     /locus_tag="PLQ70_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34707.1"
                     /translation="MTTNTILLLLLSLLIAGSLSFFQYLYKARIKSKVSLVLAFLRFL
                     SIFGLLLLLINPIMSRKTLETIKTPLPIVVDNSSSIVDLKAKEMALELFKKLSANKEL
                     QEKFEVQYYRFDSEFQQSDEFDFKGTQTNLDEVAKSLKSIYKNTVFPTVLITDGNQTS
                     GNDYVYSFDANNKIYPLIVGDTTTFLDLKVTQLNVNKYAFHKNKFPVEVFLNYSGNKN
                     ITADFRISQGNSVLSKQSVSFTPSKKSMILNVLLPADKVGLQVFKATISSKEKEKNSY
                     NNTKNFAVEIIDQKTNVAIISAINHPDIGALKRAIESNAQRKATVIKPNSINSLQDYN
                     VLILYQPTNEFKSFFEKNKVAGINTFIVTGNNTDFNFLNQQQPNLVFKMSGQKEDYLA
                     EFNSQFNLFAIDNIGFENFPPLQNPFGTITTNGTVSTLLTSKIRNIDTNASLLAFVEN
                     QGKRSAFLLGENVWKWRLQSHIDHQSFEKFDVFVDKIIQFLASNDSKKSLVVNHESFY
                     NSGEAIEILASYFNKNYEFDEKARLTISVTNTKTKQAKNYDLLKGNNSFKVNLDGLSA
                     GSYNFTVKELNSKTSYSSHFEILDFDIEKQFVNPDVQTLNQLASQTQGKVYFPDQVDS
                     LIKTLLENENYKALQKEISTKTPLIDWVWLLVLIAIILAAEWFLRKYNGML"
     gene            complement(3024..3770)
                     /gene="fabG"
                     /locus_tag="PLQ70_08585"
     CDS             complement(3024..3770)
                     /gene="fabG"
                     /locus_tag="PLQ70_08585"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HQE34708.1"
                     /translation="MKLLEGKVAIITGASRGIGRGIAEVFAKHGANVAFTYSSSVESA
                     QALENELNAMGIKAKGYQSNAADFNEAQTFVDAVLAEFGTVDILINNAGITKDNLLMR
                     MSEADFDQVINVNLKSVFNMTKAIQKTFLKQRAGSIINISSVVGVSGNAGQTNYAASK
                     AGAIGFTKSVALELGSRNIRCNAIAPGFIETEMTAKLSEEVVKGWREGIPLKRGGTTE
                     DVANACLFLASDMSAYVTGQVLNVCGGMLT"
     gene            complement(3901..4293)
                     /locus_tag="PLQ70_08590"
     CDS             complement(3901..4293)
                     /locus_tag="PLQ70_08590"
                     /inference="COORDINATES: protein motif:HMM:NF027791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5053 domain-containing protein"
                     /protein_id="HQE34709.1"
                     /translation="MLDEIKKLKEKYQNTDNENEIADIHSEMNLLFAENPDTFADSLL
                     KCIKESNKKADELLLKQKLEDILPVISVSYLAKNYFKKTPQWFYQRLNGNNVNGKEAH
                     FTHKELETLAIALKEIGQKLSASASLIL"
     gene            complement(4692..4973)
                     /locus_tag="PLQ70_08595"
     CDS             complement(4692..4973)
                     /locus_tag="PLQ70_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34710.1"
                     /translation="MNKKEPKSAYSILSNQRELAKNKLRDLIVSSASKKEIKEAQAAF
                     DALDEKVKKIDRAVGDYSKEVGNYSETNLSFFRFIGVYFLFNKIWNLFK"
     gene            complement(5044..5835)
                     /locus_tag="PLQ70_08600"
     CDS             complement(5044..5835)
                     /locus_tag="PLQ70_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34711.1"
                     /translation="MGCDISILSKHNLNISNVETLAIDLSNRFGFTIEYGYYANADYN
                     QLLQNGLKEDFISLGLIDKQPFVKKYKLIDEKFQQKQLYKKFGESLFDKKEYWWWYDD
                     DEMPSQERIEEEKKEFHITNYFLDIHSENAESSYLTIYDEIVSSDLHYYSRWWRFCDT
                     IQLRDNFENRYFQNFRKSVMKDTLLLGGEKAYFVNDQCNHLNGVGQGSENEYNWQELE
                     NYINSIETLEVISISKTVLDTHYQLDIRNREQNTLAFVDDFEDLL"
     gene            complement(5838..>6192)
                     /locus_tag="PLQ70_08605"
     CDS             complement(5838..>6192)
                     /locus_tag="PLQ70_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HQE34712.1"
                     /translation="TPFIEKADLMLSLNKDLQEQSQKFERTIQRKFELDDLPKKLQDW
                     YVLSYSEFIKELAKKKIKLSLSQEAEWEDYFTTEAQKVLQLKTQIDTTDKAIDKMVYE
                     LYGLSEDEIAIVEKS"
BASE COUNT         1983 a         1109 c          870 g         2230 t
ORIGIN      
        1 aaaatcaaga atcgtttcat ttaaaaacgt tttaattgtg acgcattgaa agtccattct
       61 ggagttttca acgtagaacc aaaatcggta tcataccaag gatttattga aacgttattg
      121 ggatttttca atatttgaag ttcctgtgct ggcatataac tttgtgccaa aagcatcact
      181 ttttggttgg ttttagggtt tacggcgaca tcaacaacaa taactgcgtg accaggaaag
      241 cctcctttta taaaaacatc accaattttc acatcagaaa ctttaattgt ttttagctct
      301 ttttctaagg aagctgttcc tgcatacata aaaacggttt ccaaatagtt ccaataggtt
      361 tcgtaactaa ccgacggttt tgccgttttt acccagctcg ttttgttgcc tttgacagca
      421 attctataac ccgccgccca tttactaaaa tcaactcgaa aaccatttgt aaaattaaaa
      481 tgtattttgt catattgctt gaggctataa aaataatccg ctcttagacg cattacagca
      541 tcggcacatt ggtgtaaatc ctgttttcca atgggtaaat taactactgc ttcataaaca
      601 tttcgattgg attttactgt tccatcaaaa tacaaaacgt tagatccaga aggttttaaa
      661 ggaagatctc tcaaaaaaaa gccaaacgaa gactccgatt ccgcttgtct cacaaatccc
      721 gctggaggct gaaaacggtg ctggatggta ttcccataaa gattgctttt gacactatta
      781 tttgtctttg aaaatgaaaa tgcacaaaaa atagccgtca aaatcaccgc aataaacaag
      841 aatttgattt tcataatttc gacttttagt ttatttctaa aatgaaaaaa acttgaatat
      901 attgtattaa tcacaacatc ccgttatatt tcctcaaaaa ccactcggca gccaaaataa
      961 tagctatcaa aaccaacaac caaacccaat caatcaaagg cgttttggta ctaatctctt
     1021 tttgaagagc tttataattt tcgttttcta aaagtgtttt aattaaagaa tctacttggt
     1081 ctggaaaata aactttccct tgagtttgtg aagccaattg gtttaacgtt tgaacatcag
     1141 gattgacgaa ctgcttttca atatcaaaat ctaaaatttc aaaatgactg gaataactag
     1201 tttttgaatt taattctttg accgtaaaat tataagaacc tgccgaaagt ccgtctaaat
     1261 taaccttaaa cgaattatta cctttcaaca agtcataatt tttggcttgc ttggttttgg
     1321 tattcgttac cgaaatagtc aaacgcgctt tctcatcgaa ttcgtaattt ttattgaaat
     1381 aagaagctaa aatctcgatg gcttctcctg aattataaaa actttcgtga ttgaccacca
     1441 acgatttttt ggaatcatta gaagccaaaa actggatgat cttatctaca aaaacatcaa
     1501 acttttcgaa agattgatga tcaatatgac tttgtaaacg ccatttccaa acattttctc
     1561 ccaataaaaa agccgaacgt ttgccttgat tttcgacaaa agccaataaa gaagcatttg
     1621 tgtcaatatt ccttattttg gaagtaagca aagtcgaaac agttccattt gtggttatcg
     1681 ttccaaaagg attttgcaaa ggcgggaaat tttcaaaacc aatattatca atcgcaaaaa
     1741 gattgaattg cgaattaaat tcggccaaat aatcttcttt ttgaccactc attttaaaaa
     1801 ccaaattggg ttgctgttgg tttaaaaaat tgaaatctgt gttatttcct gttacaataa
     1861 atgtattaat tccagctact ttattctttt caaaaaacga tttaaattcg tttgtcggtt
     1921 gatacaaaat caaaacattg taatcttgta atgaattgat tgaatttggt ttaataacag
     1981 ttgcttttcg ttgtgcatta gactcaatcg cacgtttcaa agctccaata tcaggatgat
     2041 ttattgccga aattatagca acattcgttt tttggtctat gatttcaacg gcaaaattct
     2101 tggtattatt ataactattt ttctcttttt ctttggaaga aattgtcgcc ttaaaaactt
     2161 gtaaacctac tttgtcggcg ggaagcaaaa cgttcaaaat catcgatttt ttggagggtg
     2221 taaacgaaac gctttgtttg ctcaaaaccg aatttccttg cgaaatacga aaatcagccg
     2281 tgatgttttt gttgccagaa taattcaaaa atacttctac cggaaattta ttcttgtgaa
     2341 aagcatattt attgacgttc aactgtgtta cttttaagtc taaaaaagtc gttgtgtcgc
     2401 caacaatcaa agggtaaatt ttattattag catcaaaaga atagacataa tcatttccag
     2461 aggtttgatt tccatcagtt atcaaaaccg ttggaaaaac ggtattttta tagatgcttt
     2521 tcaggctttt ggctacttca tctaaattgg tttgagttcc tttgaaatca aattcgtctg
     2581 attgttgaaa ttcactgtca aatcgatagt attgaacctc gaatttctcc tgtaattctt
     2641 tgttggcaga aagctttttg aacaattcta aagccatttc tttggctttc aaatcgacaa
     2701 ttgaactgga gttatccacc acaatcggca atggtgtttt gatagtttcc aatgtttttc
     2761 gactcattat tggatttatc aacaacaata atagtccgaa aatggacaaa aaacgcaaaa
     2821 aagccaaaac caagctgact ttcgacttga ttctggcctt gtataaatat tgaaaaaacg
     2881 acaaacttcc agctattagt aaagaaagca atagtaatag aattgtgttt gtagtcattt
     2941 tttttgatta aaagattgaa aaattgaaag atttaaaaat ttcaacaatc tttaaatctt
     3001 tcaatcataa aatcattaaa ttgttaagta agcattcctc cacaaacatt caatacttgt
     3061 cctgtaacat aagcactcat atccgaagcc aagaaaagac aagcattagc aacatcttca
     3121 gtcgttcctc cacgtttcaa cgggattcct tctctccaac cttttaccac ctcttcgctt
     3181 aactttgcag tcatttcagt ttcaataaat cctggagcga tagcgttgca acgaatgtta
     3241 cgtgaaccca attccaaagc tacggattta gtaaaaccga tggcacctgc tttggaagcg
     3301 gcataattcg tttgacccgc gtttccagaa actccaacaa ccgaactaat attgatgatg
     3361 gaacccgcac gttgtttcaa aaacgttttt tgaatcgctt tggtcatatt gaaaaccgat
     3421 ttcaaattca cgttgattac ttggtcaaaa tcagcttcgg acatacgcat caacaaatta
     3481 tctttggtaa ttcctgcatt attgattaaa atatcaaccg ttccaaattc tgccaaaaca
     3541 gcatccacaa aggtttgggc ttcattaaaa tcggcagcat tcgattgata tcctttggct
     3601 ttgataccca ttgcattcaa ttcattttcc aaagcttgag ccgattctac agatgaactg
     3661 taagtaaacg ccacattggc accgtgtttt gcaaaaactt ctgcgattcc tcttcctatt
     3721 ccgcgacttg cgccagtgat gatggctact tttccttcga gtaatttcat attctaaaat
     3781 tagtgtgatt attttttgta ttacaaatat agattattta agcgttttat aaaatttgtt
     3841 ctataaaaaa agccttaccg aagtaaagct tttaaaattt tgaatctata gttgtaatta
     3901 tcagagtatg agtgatgccg atgcgcttaa tttttgtcct atttctttta aagcaatagc
     3961 taatgtttct aattctttgt gcgtaaaatg agcttctttg ccattgacat tattaccatt
     4021 tagtctttga taaaaccatt gtggtgtttt tttgaaataa tttttagcca aataagaaac
     4081 tgatattaca ggcaaaatat cctccagctt ttgctttaac aacaattcat ctgctttttt
     4141 attgctttct tttatacatt ttaataatga atccgcaaac gtatcaggat tttcggcaaa
     4201 caacaaattc atttcactgt gaatatcagc gatttcgttt tcgttatcag tattttgata
     4261 tttttctttt agttttttga tttcgtctaa cataactttc tcttttaaat tattgtaatg
     4321 ttactttcca agttctttta gtttttgaac taattttttg atttctaaat ccaccacttt
     4381 tttcatttgt ggacttcgac tggataattc tttgtaaaat tccagataga ataataaatc
     4441 tttttaaagt ttgttcttct cgatttggaa ttgttctcta tcattcataa cttgattttt
     4501 caaaattaat aacatttttg ttattaaaca aatggtttaa cattttactt taaaaataac
     4561 caaagtagaa aaaatattat tcctactcca gcgattacat cataaatgga aaattcaatt
     4621 tcaaattcat aatcaatttc cttttctaac agattttaaa ataatgcctt aattattaag
     4681 caattatatc ctcatttaaa aagattccaa attttgttaa atagaaaata aacgccaata
     4741 aatctgaaaa aacttaaatt tgtttcagaa taattgccta cctctttaga ataatcaccc
     4801 actgccctat ctattttttt tactttttca tctaaagcat caaatgctgc ttgtgcttct
     4861 tttatttctt tcttagaagc agaactaaca attaaatcac gcaacttatt ttttgccagc
     4921 tctctttgat ttgacaaaat actgtaagcc gatttcggtt cttttttgtt catatttttt
     4981 gagatataaa aattcaagga tacaattcct taagttgtaa atcaattata attttaaaaa
     5041 ttattaaagt aaatcttcga aatcgtcaac aaaagcaagc gtattttgct ctctatttct
     5101 tatatctaat tgataatgtg tatctaaaac tgtttttgaa attgaaatca cttctaaagt
     5161 ttcgatagaa ttaatataat tttcgagttc ttgccaattg tattcgtttt cacttccttg
     5221 ccctactcca tttaaatgat tgcattggtc atttacaaaa taggcttttt ctccaccaag
     5281 taacaaggtg tctttcatta ctgattttct aaaattttga aaatatctat tttcaaaatt
     5341 atctcgtaat tgtatcgtat cacaaaaacg ccaccatctt gaataataat gcaaatcgct
     5401 tgaaactatt tcatcataaa tagtcaaata actgctttct gcgttttcag aatgaatatc
     5461 taaaaaatag tttgtaatgt gaaactcttt tttttcttcc tcaatccttt cttggcttgg
     5521 catttcatca tcatcatacc accaccaata ttctttttta tcaaaaaggc tttctccaaa
     5581 ttttttatac aattgttttt gctgaaattt ttcatctatc aacttgtact tttttacaaa
     5641 tggttgtttg tcaatcaatc ctaatgaaat aaaatcttct ttcaacccat tttgcaacaa
     5701 ttgattgtag tctgcattgg cataataacc atattcaata gtaaatccaa accggtttga
     5761 taaatcaatg gctaatgttt ctacatttga aatattcaaa ttatgtttac tcaatatgga
     5821 aatatcacaa cccataatta acttttctcc acaatcgcaa tctcgtcctc gctcaatcca
     5881 tacaattcat aaaccatctt atcaatagct ttgtcggtgg tgtcgatttg ggttttgagt
     5941 tggagaactt tttgagcttc ggtggtgaaa taatcttccc actcggcttc ttgcgagagc
     6001 gataatttta ttttcttttt cgccagttct ttgatgaatt ccgaatacga taacacatac
     6061 caatcttgca actttttagg taaatcgtct aactcaaatt tgcgctgtat cgttcgttca
     6121 aacttttggg attgttcctg taagtctttg tttaaagaca acattaaatc ggctttttca
     6181 atgaagggag tt
//
LOCUS       DAOSNN010000161         6185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12779_length_6185_cov_4.219413, whole genome shotgun sequence.
ACCESSION   DAOSNN010000161 DAOSNN010000000
VERSION     DAOSNN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12779_length_6185_cov_4.219413"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            586..1344
                     /gene="yaaA"
                     /locus_tag="PLQ70_08610"
     CDS             586..1344
                     /gene="yaaA"
                     /locus_tag="PLQ70_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HQE34713.1"
                     /translation="MKIVISPAKSLNFEKALPTSNFTESLFLKESRQVHKVLKQKSPK
                     ELSELMDISDKLADLNWQRNKDWKTPFSPENARPAVFAFDGDVYTGLDAYSIPIEKLD
                     VLQERLRILSGLYGLLKPLDLMQAYRLEMGTKLPIGESKNLYEFWKPIIAKALNKELK
                     KGELFVNLASNEYFSAVDVKVLKVPVITPEFKDYKDGKLKIISFFAKKARGLMMRYII
                     DTNAETIEDLKGFDYEGYRFDANLSKGNQLVFTR"
     gene            complement(1420..>2165)
                     /locus_tag="PLQ70_08615"
     CDS             complement(1420..>2165)
                     /locus_tag="PLQ70_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34714.1"
                     /translation="VMCFILGFGLYGSTLIIPIYTQAILGWTATDAGLLLIPGSITTA
                     FMMPIVGNMIQKGVPQQYMVAVGFLIFFFFTFVMYNNMTPDTGTEHLFWPLILRGIGL
                     GLLFVPITTLSLSTLKGKHIGEGAAFTGMMRQLGGSFGIAIITTFITRWTQEHRVNLI
                     ANLDPSKYEVQQRIATMQRGFMSKGFSSNESLQKAYQVLDYSVLKQSTVLSYMDIFLY
                     LGILFLCCIPIILFIKKGKNKVNPADAMH"
     assembly_gap    2166..2265
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2266..3164)
                     /locus_tag="PLQ70_08620"
     CDS             complement(<2266..3164)
                     /locus_tag="PLQ70_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HQE34715.1"
                     /translation="MKIVQAADDDLVEYGFRRVIITITAVLCALLEIVDTTIVNVALT
                     DMRGSLGATLTDVAWVITAYAIANVIVIPMTSWLSQQFGRRNYFVASIILFTVCSFLC
                     GNATNIWELVAFRFVQGIGGGALLVTAQTIITESYPVAKRGMAQAIYGMGVIVGPTLG
                     PPLGGYLVDHYSWPYIFYINIPLGIIATILAISFVRSPKYGEKLKSNQVDWWGIALLA
                     TFIGSLQFVLEHGQQDDWFNDSLITSLSVVTIFGLILFIWRELTYKHPIVNLSVLKDG
                     NLRIGTVMCFILGFGLYGSTLIIP"
     gene            complement(3282..4361)
                     /locus_tag="PLQ70_08625"
     CDS             complement(3282..4361)
                     /locus_tag="PLQ70_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HQE34716.1"
                     /translation="MEKKKTNKKFIIILSALVLIGGTYGIYKYIHSLSHEHTDDAQIE
                     KKMNPIIPRVPGYISKVYVKDNDFVKKGDTLFTIDQRDYQLKIDEATAALLAAESQFE
                     AAKADIGSANASISVSDANMKSASGSIESAKIRLKQITSDYNRYNNLYKTHTITKQQY
                     EQALTAKEEAENQVRVLEQQQRASAYQKSVIESKSKASDKQTEVAAANIKRAQTLLDV
                     AKLNLTYTVVTAAIDGQVSKVDIQPGQLVQAGQSLFYIINNNEAWVVANFKETQLDKM
                     VVGQKVGLKVDAYPDYEFQGIVTSFSPATGSRFSLLPPDNATGNFVKTIQRLPVKISL
                     DTTNDPEKVKLLRPGMNVDVDVHLK"
     gene            complement(4373..5695)
                     /locus_tag="PLQ70_08630"
     CDS             complement(4373..5695)
                     /locus_tag="PLQ70_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE34717.1"
                     /translation="MQIKHLLLFGISLIGITTVTAQEKKSLTLEEAVQLAWTKSNEVN
                     LANAKVNTSKYELQAVKNNQYPDFKLSGQYQRLTHASVEMANAGSNSSAMPSPDQVML
                     AQANLSLPVFAGFKIQNNIKLSDNLFQAENANAAKTKEDITMRVVNYYTALYKAQKTL
                     DILNENQKSAKQRVTDFTELEKNGIIPRNDLLKAQLLVSRTQISIDEAQNNLNNINFY
                     LATLLKIDTNTKLEVNEADFFNLKTDNAPKTDAVALENRKDLEAVHFQQKASEANIKI
                     AKSGYYPSISLIGGYTALDLKDIVTVKYAMNFGVGLSYDIGGILKNKAHVKAAESKAL
                     EVKNSEAILTDRIKNEVQKALEDYDLAINQSVVYEEAVQQASENYRIVKDKFDNGLSD
                     TNDLVEADVEFLGAKLNTAVSKATIIQKYYELLSVTGQIAQSFNLSKI"
     gene            complement(5685..>6185)
                     /locus_tag="PLQ70_08635"
     CDS             complement(5685..>6185)
                     /locus_tag="PLQ70_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQE34718.1"
                     /translation="MVSYYFGSKEKLLESLVLFKTSGLKEQLVNLIDENLEPLEKINK
                     LIALYINRLNCNRGIYRVLHFELASKKRVLELESFTEIKKSNLKSLELIIQEGQAKGV
                     FRKDIIIPLLTPTILGTYFHFQMNKPFFEEILNLKTDEEFNHYVKTDLTKHIQQTIKA
                     LLIYAN"
BASE COUNT         1797 a         1092 c         1156 g         2040 t
ORIGIN      
        1 tcggaaaaca aaatcccttt caagttgttg taaacggcta attctccatt gctattacta
       61 gaaagcaagg ttttaatcaa ttgatttttg ttgacaaaat cagatagaag gatgttccaa
      121 tggggtttgt tattcattgt taaagggatg atttgaaggc tggtattttc ttttgtaaaa
      181 ctaaaaaata atttgtgcta attggtgaaa tttgtggtaa aaaaatagca cgcagatgag
      241 gcggattttg gcgaataaaa cggattttct atctgtgtaa attagtttta ttcgttggaa
      301 tctgtgggtt aaaattagaa tagtatagaa atacattaaa ggatttttaa acacatagag
      361 acatagaaaa aaagaattgg atagccccaa atctgggttt cacatagcta tgattttctt
      421 tgataaagtg aaatgtcttt caaaattaac acgaactatg tttctatgtg tttaaaaatt
      481 gattcaaatg gaataggtcc actttgagga ataaaaatca atcttaattt tcgttttcgg
      541 attttcaatt tgtacttttg aaatcgtttt aaaccaaatt gaaaaatgaa aatcgtcata
      601 tcgcctgcca agtctttaaa cttcgaaaaa gcattgccaa catcgaattt tacggaatct
      661 ttgtttttga aagaatcacg tcaagtgcat aaagttttga agcaaaaatc accaaaggaa
      721 ctttcggaat taatggatat ttcggataaa ttggccgatt tgaattggca acgcaataaa
      781 gattggaaaa cgcctttttc tcctgaaaat gcacgtcccg ccgtttttgc tttcgatggt
      841 gatgtttata cgggtttgga tgcctattcg attccgattg aaaaattaga cgttttgcaa
      901 gagcgtttgc gcattttgtc gggtttgtac ggactcttga aacctttgga tttgatgcag
      961 gcttaccgat tggaaatggg aaccaaatta cccattggcg aaagcaaaaa tttgtatgaa
     1021 ttttggaagc ctattatcgc caaagcctta aacaaagaac tcaaaaaagg ggagttgttc
     1081 gtgaatttgg ccagcaatga atatttctcg gctgtagatg tcaaagtctt gaaagtgcct
     1141 gtaatcactc cagaattcaa ggattacaaa gacggaaaac taaaaataat cagttttttt
     1201 gccaagaagg cgaggggctt gatgatgcgt tacatcattg acacgaatgc cgaaaccata
     1261 gaagacttga aaggtttcga ctatgaaggt tatcgttttg acgccaattt gtctaaagga
     1321 aatcaattgg tttttacgag atagctaaaa aaagaccgca gattagcaga tttaaaatta
     1381 ataatctgcg aatctgcggt tttagttttt actaaaatct tagtgcattg catcagcagg
     1441 atttacctta ttttttcctt ttttgataaa tagaataatc gggatacaac ataaaaataa
     1501 gattcctaaa taaaggaaaa tatccatata cgataaaaca gtactttgtt ttagaacaga
     1561 ataatccaat acttgatagg ctttttgcaa agattcgtta ctgctaaaac ctttggacat
     1621 aaagcctctt tgcatagtag caattcgttg ttgtacctca tatttacttg gatctaaatt
     1681 ggcaattaaa tttacacgat gttcttgtgt ccaacgggtg ataaatgtgg taatgatggc
     1741 aataccaaac gaacctccca attgacgcat cattcctgta aaagcagctc cttcgccaat
     1801 gtgttttcct tttaaagtag aaagcgaaag tgtagttatg ggaacaaaaa gtaatcccaa
     1861 tccaattcct cttaaaatta atggccaaaa taaatgttct gttcctgtat cgggagtcat
     1921 attattgtac attacaaacg taaagaagaa gaaaattaaa aaaccaacgg ctaccatata
     1981 ctgttgtggc acaccttttt gaatcatatt ccccacaatt ggcatcataa acgcagtggt
     2041 tatcgaacct ggaattaata ataatcccgc atcagtagct gtccagccca agatggcttg
     2101 tgtgtaaatt gggataatca aggtagaacc atacagtccg aaacccaaaa taaaacacat
     2161 aacggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngggat aatcaaggta
     2281 gaaccataca gtccgaaacc caaaataaaa cacataacgg ttcctattct caggtttcca
     2341 tcttttaata cacttaaatt tacgatggga tgtttgtagg tgagttctct ccaaataaag
     2401 agtatcaacc caaaaatggt tactacgctc aagctggtaa tcaaggaatc attgaaccaa
     2461 tcgtcttgtt gtccgtgttc taaaacaaat tgcaaagatc ctatgaaagt ggccaacaag
     2521 gcaattcccc accaatcgac ctggtttgat tttaatttct caccatattt aggacttctc
     2581 acgaaagaaa tggccaaaat tgtggcaatg attcccaacg ggatattgat gtaaaaaatg
     2641 taaggccacg aataatgatc gaccaaatat cctcccaatg gcggacctaa tgtcgggcca
     2701 acaattactc ccattccgta aatagcctgc gccattccac gtttggcaac gggataactt
     2761 tcggtaatga tggtttgggc ggttaccaac aaggcgccac caccaatacc ttgaacaaat
     2821 ctgaaggcta ctaattccca aatattggtt gcgtttccgc acaaaaagga acagaccgta
     2881 aaaagaataa tggaggctac aaagtaatta cgtcttccga attgttgcga taaccaactc
     2941 gtcattggaa tcacgattac gttggcaatg gcataagccg tgattaccca agccacgtcg
     3001 gtcaaggtag cgccaagact ccctcgcata tcggttagcg ccacgttgac gatggttgtg
     3061 tccacgattt ccaacagcgc acaaagcact gccgtaatcg tgatgatgac gcgtcgaaat
     3121 ccgtattcaa ctaaatcgtc gtctgctgct tgtactattt tcatttgtat gaattataaa
     3181 gtatgtgttt taattcagtt attagtcatt agtaaagagt gattagcttc aggctaatta
     3241 ctaacagcta ttcactaatc actgaacagt tatccaaaat cttacttcaa atgcacgtcc
     3301 acgtccacgt tcattcccgg gcgcaataat tttactttct cgggatcgtt tgtcgtgtcg
     3361 aggctaattt ttactggcaa tctttggatg gttttcacga agtttccggt agcattatct
     3421 ggtggcaaca aggagaaacg agaacctgtt gctggcgaaa aggaagtcac gattccttgg
     3481 aattcataat caggataagc atctactttt aaacctactt tttgtcccac aaccattttg
     3541 tctaattggg tttctttgaa attagccacg acccaagctt cgttgttgtt gatgatgtaa
     3601 aacaaggatt gtcctgcctg aaccaattgt ccgggttgaa tgtccacttt ggaaacttgt
     3661 ccgtcgatgg cggccgtaac cacggtgtaa gtcaggttta atttggccac gtccaacaaa
     3721 gtttgggctc tcttgatatt ggctgcagcc acttcggttt gtttgtccga agcttttgat
     3781 ttggattcaa tcaccgattt ttggtaagca cttgctcttt gttgttgctc caaaacgcgc
     3841 acctgatttt cggcttcttc cttggcagtc aaggcttgtt cgtattgttg tttggtaatc
     3901 gtgtgtgttt tgtacaaatt gttgtaacgg ttgtaatcgc tggtaatttg tttcaacctg
     3961 attttggcac tttcgataga accgcttgcc gatttcatat tggcatcaga taccgaaata
     4021 ctggcattgg cacttccaat atcggccttg gcggcttcaa attgactttc ggcagccaat
     4081 aaagcggcgg tagcttcgtc aattttcaat tggtaatctc tttggtcaat ggtgaacaaa
     4141 gtatctcctt tttttacaaa atcattgtct tttacataaa ccttgctgat gtatccagga
     4201 actctcggga taatcgggtt catttttttc tcgatttggg catcatcggt atgttcgtga
     4261 gacaaagagt gaatgtattt ataaattcca taagtgccac caattagtac caatgcactt
     4321 agtatgataa tgaatttttt atttgttttt ttcttttcca tggtgattat ttttatattt
     4381 tagaaagatt gaatgattgt gctatttgtc ctgttaccga aagtaattcg taatattttt
     4441 gaatgatggt ggctttagaa actgctgtat ttagtttagc tccaagaaat tctacatcgg
     4501 cttctaccaa gtcgttcgtg tctgataaac cgttgtcaaa tttgtctttc acgattctgt
     4561 agttttcaga tgcttgttgc accgcttctt cataaaccac gctttgattg atggctaaat
     4621 cataatcttc aagcgctttt tgaacttcat tcttgatgcg gtcggtcaag atggcttcgg
     4681 aattttttac ttccaatgct ttgctttcgg ctgcttttac atgggcttta ttttttaaaa
     4741 ttcctccgat gtcatacgaa agacccacac caaaattcat cgcatatttg acggtaacaa
     4801 tatcttttaa atctagtgca gtataacctc caatgagtga aatggatgga taatatcctg
     4861 attttgctat tttgatattt gcctcgctgg ctttttgttg aaaatggaca gcttctaaat
     4921 cttttctatt ttctaaggcc acagcatcgg tttttggtgc attatcggtt tttagattga
     4981 aaaaatcagc ttcattaact tccaatttgg tgttcgtatc tatttttagc aaagtggcga
     5041 gatagaaatt gatgttgttg aggttatttt gggcctcatc aatagaaatt tgggttcttg
     5101 aaaccagcaa ctgagctttc aataaatcat ttcttggaat aattccattt ttctccagtt
     5161 ctgtaaaatc agtgacacgt tgtttggcac ttttttgatt ttcattcaat atgtccaatg
     5221 ttttttgtgc cttgtacaaa gcggtgtaat aatttaccac tcgcatcgtg atgtcttctt
     5281 tggttttggc ggcattggca ttttcagctt gaaacaaatt atccgataat ttgatattat
     5341 tctgaatttt gaatcctgca aatacgggca agctcaagtt ggcttgtgca agcatcactt
     5401 ggtctggcga aggcatagca cttgaattgg aacctgcatt ggccatttct accgaagcgt
     5461 gggtcaaacg ttggtattgt ccagataatt taaaatcggg atattggttg tttttaacag
     5521 cttgcaattc gtatttcgac gtatttactt tggcattggc caagtttact tcgttgcttt
     5581 tcgtccaagc cagttgaacc gcttcttcca aggttaaact ctttttttct tgtgctgtta
     5641 cagttgttat tcctatcaaa gaaataccaa aaagtaataa atgtttaatt tgcatatatc
     5701 aaaagtgctt ttatggtttg ttgaatgtgt tttgttaaat cggtttttac gtaatgatta
     5761 aattcttcat ctgtttttaa gtttaatatt tcttcaaaaa aaggtttgtt catttgaaaa
     5821 tggaaataag tgcccaatat tgttggtgtt agcaaaggga tgataatatc ttttctaaaa
     5881 actcctttgg cttgaccttc ttggataatg agttccaatg attttagatt ggattttttt
     5941 atttctgtga aagactccaa ttctaatact ctttttttag aagctaattc aaaatgcaac
     6001 acgcggtaaa ttcctctgtt gcaattgagt ctgttgatgt aaagtgcaat tagtttgttt
     6061 attttttcta aaggttctag attctcatca atcaaattaa ctaattgttc cttgagtccc
     6121 gaggttttaa ataacaccaa cgattccaat aatttttctt tactgccaaa ataataggaa
     6181 accat
//
LOCUS       DAOSNN010000162         6127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_12946_length_6127_cov_4.840250, whole genome shotgun sequence.
ACCESSION   DAOSNN010000162 DAOSNN010000000
VERSION     DAOSNN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12946_length_6127_cov_4.840250"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PLQ70_08640"
     CDS             <1..593
                     /locus_tag="PLQ70_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HQE34719.1"
                     /translation="WIAKMIKDFPELEFIGGGEESFGYMVGDAVRDKDAVAATLLICE
                     LAAQVKANGSSVYKELQHLYVEHGFYKEHLISLTKKGMDGLAEINKMMVSLRENPMKE
                     INGQRVIMLEDYKSSVAKNLLTGEEETMDMPKSDVLIYYTEDGSKICARPSGTEPKIK
                     FYISVNTELDSVENFTKVEAVLDNKIKNIIAAMQLS"
     gene            678..2504
                     /locus_tag="PLQ70_08645"
     CDS             678..2504
                     /locus_tag="PLQ70_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE34720.1"
                     /translation="MSNYKKIVPFIIPYKKYAFLNIFFNILYAIFGTLSFVSLMPMMD
                     VLFGQSKKNYILPVYKGISGLKTYLSDYLSYYLTSITDTNGIGYTLSILVAGIITIFL
                     LKNLCDYLAMFFITFLRNGVLRDMRNAMYKKTIDLPLAFYSEKRKGDVIARISGDVNE
                     VQTSFLSILELIVKEPLTIIFTLIAMVTISLQLTLFVFIFIPVSGYIISLIGKQLKRK
                     STKAQEEQGQFLSTIEETLGGLKVVKGYNSENYFNQVFQESTQRFFTLSNSIGNRQNL
                     ASPASEFMGIMVIAILLWYGGHMVLIEHSLKGAAFITYMGLAYNILTPAKSISKASYA
                     IKRGNAAAERILEILEQENPITSKANAVQKNTFNSEISIKNINFKYEEDYVLKNFSIE
                     VKKGQTVALVGQSGSGKSTIANLLTRFYDVNEGTIAIDSVNIKDLNLQSLRGLMGLVT
                     QDSILFNDTIKANIALGKLDATDDEIIEALKIANAYEFVKNLPQGIYTNIGDSGNKLS
                     GGQKQRLSIARAVLKNPPIMILDEATSALDTESEKFVQVALENMMQNRTSIVIAHRLS
                     TIQKADKIVVMQKGEIVEQGTHEELIALNGTYNKLVMMQSLE"
     gene            2601..3302
                     /locus_tag="PLQ70_08650"
     CDS             2601..3302
                     /locus_tag="PLQ70_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HQE34721.1"
                     /translation="MYLNNKNITLPEDPDTVVWKYLDLSKFLDLLLSKKLFMSRSDKF
                     EDQYEGTFSEPTYQEIKKIAEDNPEFLHYYKSHREKVAISSWHINEYESFAMWQIFTQ
                     NSEGLAIQSTVNRLKNAFNSETQHQQYIGEVNYIDYKKEYIPFDDMFFPFLFKRKSFQ
                     YEREVRIITDVSESNIKLNEGLKINVDINQLIEKIYIHPKSENWYKNLVIQLVEQLGF
                     DFTIEKSDLESDILI"
     gene            complement(3357..5819)
                     /locus_tag="PLQ70_08655"
     CDS             complement(3357..5819)
                     /locus_tag="PLQ70_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE34722.1"
                     /translation="MGLNKIILVVLFFCFGVVTSAQTARIKGVILDKNNQPVANANVS
                     CQNNGVQSNVNGFYILTVPANQKVVVVFSHVSLKKATVAVTLKSDEDYEFNLVMNDKD
                     EQMGEVIVTVNNKKRVQGITTIEPATIRKIPGANAGVENILKSMPGVNSNNELSTQYA
                     VRGGNYDENLVYVNEIEVYRPFLIRSGQQEGLSFTNTDLVQNIDFSAGGFQAKYGDKL
                     SSVLDITYRTPTQFGAVAEASFLGASVAVDAVSKNGKWSEVSGIRYRNNSLLVNSQET
                     QSNYTPTFADFQTNVNFKPSDKLEFSFLGNISSNDYKYQPLTRQTNFGTIDDPMALLI
                     YYEGQEKDKYTTVFGAVKTTYTPSKNATYKLIASAYHTQEQEYYDILAQYRLGEVDAN
                     LGSENFGDVKFTRAIGGQLNHARNDLDALIVNAELKGIHNWKKNQFEWGAKFTRESIR
                     DRVIEWEVIDSAGFSINPPKILLKNDQPYMPYTGPLVPYQNVRATNFVDINRFSGYVQ
                     WSRKGYIGDNEIWMNAGVRSQNWTVSGDGIASSTQFTFSPRAQFSIKPNWNKDMFFRL
                     SGGLYHQPPSYRELRDADGVVQPEVKAQQSIHIVLSNDYSFKMWERPFKMVSEIYYKA
                     LTDVNPYTLDNVRIRYAAANNAKAYAQGFDFRLNGEFVPGTESWFSFGYLKTEENRDG
                     KGYISRPTDQRLKFALLFQDYMPKIPSIKLYLNLVYNTGLPGGSPSYSDPYLYQSRLN
                     DYRRADVGFSKVLIDEQNPSTKRWLQPFKELAIGLEIFNLFDNQNAITNTWVRDVYSK
                     NQYAIPNYMTTRVFNVKVNMRF"
     gene            complement(5831..>6127)
                     /locus_tag="PLQ70_08660"
     CDS             complement(5831..>6127)
                     /locus_tag="PLQ70_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HQE34723.1"
                     /translation="DVIPPKISIAKPIEGKWLTNNKYIQFTIADDLSGIKSYNGYLNG
                     KWILFEYDNKTKKIIHNFSDGIVAEGANELKIIVVDNCGNSTIFETQFFRSQKQ"
BASE COUNT         2042 a         1084 c         1081 g         1920 t
ORIGIN      
        1 aatggattgc caaaatgatt aaggatttcc cagaattgga attcattggt ggtggcgaag
       61 aaagttttgg atatatggta ggcgatgccg ttcgtgataa agatgccgtt gctgctactt
      121 tattgatttg cgaattggca gctcaggtta aagccaacgg aagttcggtt tacaaagaat
      181 tacaacattt gtatgtggaa cacggttttt acaaagaaca tttgatttcc cttaccaaaa
      241 aaggaatgga tggtttggca gaaatcaaca aaatgatggt ttctttgaga gaaaatccaa
      301 tgaaagaaat caatggtcaa cgtgtgataa tgttggaaga ttacaaatca tcggttgcca
      361 aaaacttact tactggcgaa gaagaaacga tggatatgcc aaaatcggat gtattgattt
      421 attatactga agatggttct aaaatttgtg ccagaccaag cggaacagag cctaaaatta
      481 aattttacat cagtgtcaac accgaattag atagtgtgga gaatttcacg aaagtagaag
      541 ctgttttaga taataaaatt aaaaacatta ttgcagcaat gcagttgagc taaaagacta
      601 ttactccaaa aataaaaaca aacccgacat tttttaaaat tgtcgggttt taatgcctta
      661 acaacaaccg aaaataaatg agtaattaca aaaaaatagt tccttttata attccctaca
      721 aaaaatatgc tttcctaaac atatttttca acattctgta tgctattttc ggaacacttt
      781 cttttgtttc gcttatgcca atgatggatg ttttgtttgg tcaatcgaaa aaaaattata
      841 ttcttccagt ctataaagga atttcaggat taaaaaccta tttatctgat tatttgagtt
      901 attatttgac ttcaattaca gatactaacg gaattgggta taccttatcc atattggtag
      961 cgggcatcat tacaatcttt ttattgaaaa acttatgtga ctatctcgct atgtttttta
     1021 ttaccttttt gcggaatggt gttttaagag atatgcgaaa tgctatgtac aaaaagacaa
     1081 ttgatttgcc cttggcgttc tattctgaaa aaagaaaagg agatgtaatt gctagaattt
     1141 ctggtgatgt caatgaagtt caaacttctt ttttatcgat tttagaactt attgtaaaag
     1201 aacctttgac tattattttt acattaattg caatggttac catcagttta caattgactc
     1261 ttttcgtttt tatattcatt cccgtatcgg gatacattat ttcacttatt ggaaaacaat
     1321 taaaaagaaa atcaaccaaa gcacaggaag aacaaggtca atttttatct accattgaag
     1381 aaacccttgg cggattaaaa gtggtaaaag gatataattc tgagaattat tttaaccaag
     1441 tttttcaaga atcgactcaa cgatttttta ctttatcgaa tagtattggt aaccgtcaaa
     1501 acctagcttc accagcaagc gaattcatgg gaattatggt tatcgccatt ttgctttggt
     1561 atggaggcca tatggtttta atagaacaca gtctaaaagg tgctgcgttt ataacttata
     1621 tgggattggc ttacaacatt cttactccag caaaatctat ttcgaaagct tcttatgcca
     1681 tcaaaagagg taacgcagct gcagaacgaa ttttggaaat tttggagcaa gaaaacccaa
     1741 ttaccagcaa agctaatgcg gtacaaaaaa acactttcaa ttctgaaatt agtatcaaaa
     1801 atatcaactt caaatatgaa gaggactatg tcctaaaaaa cttttcaatc gaagtgaaaa
     1861 aaggacaaac cgtggcatta gttggtcaat cgggaagcgg aaaaagtacg attgccaatt
     1921 tattgactcg tttttatgac gtcaatgaag gaacaattgc cattgattca gtcaatatta
     1981 aagacttgaa tttgcaatcg cttcgtggtt tgatgggatt ggtgactcaa gatagcattt
     2041 tgtttaacga taccataaaa gctaatattg cacttggaaa attggacgct accgatgacg
     2101 aaattatcga agccttaaaa attgccaatg cgtatgagtt tgtcaaaaat ttgcctcaag
     2161 gaatctacac caacattggc gacagcggaa acaaactttc gggtggacag aaacaacgtt
     2221 tgtcgattgc tcgtgccgtt ttgaaaaatc caccaattat gattttagac gaagctacct
     2281 cagctttgga taccgaaagc gaaaaatttg tgcaagtggc attggaaaat atgatgcaaa
     2341 accgaacttc catcgtgatt gcacaccgcc tttcgaccat tcaaaaagcc gataagattg
     2401 tggtgatgca aaaaggagaa atcgttgaac aaggaactca cgaagaactg attgccttaa
     2461 acggaactta taacaaactg gtaatgatgc agtcacttga ataatacttt cgatgaaaca
     2521 ctgatgaaac ggatttgcta aagcaaaaac acagattttg acgaattttt caattcttaa
     2581 atcttttagg ttaactaaaa atgtatctca acaacaaaaa cataacactt ccagaagatc
     2641 ccgacactgt tgtttggaaa tacttggact tatctaaatt tctcgatttg ttgttgtcga
     2701 aaaagttgtt tatgtctcgt tcggataaat ttgaagacca atacgaaggc acttttagtg
     2761 aaccaaccta ccaagagatt aaaaaaattg ctgaggacaa tcctgaattt ttgcactatt
     2821 acaaatcaca tcgagaaaaa gtagccatca gtagttggca catcaacgaa tacgaatcgt
     2881 ttgcgatgtg gcagattttt actcaaaaca gtgaaggatt ggcgattcaa tcgaccgtga
     2941 accgattgaa aaatgctttt aattctgaaa cgcagcacca acaatacatt ggcgaggtca
     3001 actatattga ttacaaaaaa gaatacattc cgtttgacga tatgtttttc ccgtttttgt
     3061 tcaagcgcaa aagttttcag tacgaaagag aagttcgcat cattacggat gtcagcgaaa
     3121 gcaacatcaa actaaatgaa ggtctaaaaa taaatgtaga tatcaaccaa ttaatcgaaa
     3181 aaatctacat tcatcccaaa tccgaaaact ggtataaaaa cctcgtaatt caattagtgg
     3241 agcaattggg ttttgatttc accatagaaa aatcagattt agaaagtgat attttgattt
     3301 aaaacacttt taaaaatgac cgcagattcg cagattattt cgatttaaac tgcaaatcaa
     3361 aacctcatat tcaccttcac gttaaaaact cgtgtggtca tataattagg aatcgcatat
     3421 tgatttttgg aatagacatc acgaacccaa gtattggtta tggcgttttg attgtcgaaa
     3481 aggttgaaaa tttccaatcc aatggctaat tccttgaaag gttgcaacca tctttttgtg
     3541 gaaggatttt gttcatcaat caaaacttta gagaaaccta catcggcacg acggtaatca
     3601 ttcaaacgac tttgatacaa ataaggatcg gaataagaag gcgaacctcc cggtaatccg
     3661 gtgttgtaaa ctaaattcag gtataatttt atgcttggaa tcttcggcat ataatcttgg
     3721 aacaaaaggg caaatttcaa tcgttggtcg gttggtcttg aaatatagcc ttttccatct
     3781 ctgttttctt cggttttcag ataaccaaaa ctaaaccaag attctgttcc aggaacaaat
     3841 tctccgttca atctaaaatc aaacccttgt gcataagctt tggcattatt ggcggcggca
     3901 taacgaattc gaacattatc caaagtataa ggattgacat cggtcaatgc tttataataa
     3961 atttcggaaa ccattttgaa aggacgctcc cacatcttga aactataatc gttgctcaaa
     4021 acaatatgta tggattgctg tgctttgacc tcgggctgca ctactccatc ggcatcacgc
     4081 aactcacgat aagacggtgg ctgatggtat aatcctcccg aaagtcggaa aaacatatct
     4141 ttattccaat tcggttttat ggagaattgc gctcttggac taaaagtaaa ttgggttgaa
     4201 cttgcaattc catctccgga aaccgtccaa ttttgtgaac gaacacccgc attcatccaa
     4261 atttcgttgt cgccaatgta gccttttcta ctccattgta cataacccga aaatctatta
     4321 atatcaacaa aattggtcgc tctcacattt tgatacggaa ccaatggtcc ggtgtaaggc
     4381 atataaggtt ggtcgttttt gagcaaaatt tttggtggat ttatagaaaa tcctgccgaa
     4441 tcaatcactt cccattcaat cactcgatca cgaatagatt cacgggtgaa ttttgctccc
     4501 cattcgaatt gatttttttt ccaattatga attcctttta gctcggcatt tacaattaaa
     4561 gcgtctaaat catttcgagc gtgattgagt tgtcctccaa ttgcacgagt aaatttcaca
     4621 tcaccaaaat tttcagaacc caaattagca tctacttcgc ccaaacggta ttgtgccaaa
     4681 atatcataat attcctgttc ttgagtatga taagccgaag caatcaattt gtaagtagca
     4741 ttttttgaag gcgtataagt ggttttcaca gctccaaaaa cggtagtgta tttgtctttt
     4801 tcttgccctt cataataaat cagtaatgcc attggatcat cgatggttcc aaaattggtt
     4861 tgtcgtgtca aaggctgata tttataatcg ttggaagaaa tattgcctaa aaaactaaat
     4921 tctaacttat ctgaaggttt gaaattaaca ttcgtctgaa aatcggcaaa tgttggtgta
     4981 taattggatt gcgtttcttg actatttacc aaaagagaat tgtttcggta gcgaattcca
     5041 gaaacttcag accattttcc gtttttggaa acggcatcaa cggcgacact tgcaccaaga
     5101 aaactagctt cggcaactgc accaaattgg gttggtgttc gataggtaat atccaaaact
     5161 gaggataatt tatcaccata tttagcttga aaacctccag cagaaaaatc tatattttga
     5221 actaaatcgg tattggtaaa actcaatcct tcttgctgac ccgaacgaat caaaaacgga
     5281 cgatagactt caatttcgtt tacataaact agattttcgt cataattgcc tccacgaacg
     5341 gcatattggg tactcaattc attattggaa ttaacgcctg gcatagattt caaaatgttt
     5401 tcgactccag cattggctcc aggaattttc cgaattgttg caggttcaat agttgtaatt
     5461 ccttgaactc tttttttgtt attgacagta acaataactt cgcccatttg ctcatctttg
     5521 tcgttcatca ccaaattaaa ttcatagtct tcatctgatt ttaaagtaac agcaacagta
     5581 gcttttttta atgaaacgtg tgaaaaaacc acaacgactt tctgatttgc aggcacagtc
     5641 aaaatataaa atccattgac attggattga actccattgt tttgacaaga cacattggca
     5701 ttggcaacag gctgattgtt tttatcgaga attacacctt ttatacgggc ggtttgagca
     5761 gatgttacta ccccaaaaca gaaaaaaagg acaacaagta ttattttatt tagacccaag
     5821 gaatttgatt ttattgtttt tgacttctaa aaaattgagt ttcaaagata gtagaatttc
     5881 cacaattatc caccacaata atttttaatt cattggctcc ttcagcaaca attccatcgc
     5941 taaaattatg tatgattttc ttggttttat tatcgtattc aaataaaatc cactttccgt
     6001 tcaaatagcc attataggat tttatacctg ataaatcatc ggcaatagtg aactgaatgt
     6061 atttattgtt ggtcaaccat ttcccttcaa tgggttttgc aatactgatt tttggaggaa
     6121 tcacatc
//
LOCUS       DAOSNN010000163         6027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13275_length_6027_cov_5.650368, whole genome shotgun sequence.
ACCESSION   DAOSNN010000163 DAOSNN010000000
VERSION     DAOSNN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13275_length_6027_cov_5.650368"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLQ70_08665"
     CDS             <1..320
                     /locus_tag="PLQ70_08665"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HQE34724.1"
                     /translation="PILFFNLDKWYTAVNYSVFIGILTLTVFKIPNVLNTFFITFLII
                     LIPFLIVNGILTGSFIDEPVVIYNNAENLGIRIGTIPVEDVAYAFSMLLMSLILIKKI
                     ESN"
     gene            481..942
                     /locus_tag="PLQ70_08670"
     CDS             481..942
                     /locus_tag="PLQ70_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HQE34725.1"
                     /translation="MKVYKKETVQHINASMEECWAFFSSPRNLQKITPTTMGFNITDF
                     DGKNMYAGQIIQYKVSPLLGLKLRWTTEITVVKDNEYFIDEQRFGPYVLWHHKHFFEP
                     TANGVKMTDVVHYALPLGFIGRIMNALVVKNKLKEIFEYRVKAVNEIFNSK"
     gene            942..2237
                     /locus_tag="PLQ70_08675"
     CDS             942..2237
                     /locus_tag="PLQ70_08675"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQE34726.1"
                     /translation="MAKQEVTLFWFRRDLRLEDNVGLFHALQSQYPVIPIFIFDEAIL
                     DSLPKNDARVGFIHESLSKINQKLEVIGSSLLVKKGKTTAVWQQLIQEFDVKEVFFNK
                     DYEPYAIKRDATICKFLEDNKISTFSFKDQVIFEEKEITKADGLPYTVYTPYKNKWLE
                     EYHSITPVPEYDATNKFSNFHKNDFAFPSLNEIGFVENPIKVIPHNLKNVATYHETRD
                     FPALNTTTYLSPHLRFGTVSIRKLVNWAVKKNQVFLSELIWREFFMQILYSFPKVVTN
                     NFKSAYDGIQWRNDEEDFKRWCSGTTGYPMVDAGMRELNQTGYMHNRVRMVVASFLCK
                     HLLLPWQWGEAYFAQKLLDYELAANVGNWQWAAGTGCDAAPYFRVFNPEIQLQKFDEK
                     GIYIRKWIPEFDLGYGQPMVEHAFARDRAIATYKAGILK"
     gene            complement(2284..2898)
                     /gene="folE"
                     /locus_tag="PLQ70_08680"
     CDS             complement(2284..2898)
                     /gene="folE"
                     /locus_tag="PLQ70_08680"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HQE34727.1"
                     /translation="MIETKVLNYEKTEQYDTETTAILADNYRSIIENLGEDVNREGLL
                     KTPERAAKAMQYLTHGYGLNPLEILKSALFTENHKQMIVVKDIEVYSMCEHHMLPFFG
                     KAHIAYIPNGKIVGLSKIPRIVDAFARRMQVQERLTDEIKNCIQSALNPLGVAVVIEA
                     QHMCMCMRGIQKQNSVTTTSSFNGAFEKNKTRKEFISLITNKLS"
     gene            complement(2930..3613)
                     /locus_tag="PLQ70_08685"
     CDS             complement(2930..3613)
                     /locus_tag="PLQ70_08685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE34728.1"
                     /translation="MKNILIIGGSKGIGNAIVLQQLEKNKVINISRNAPEISHPNLTH
                     HSLDVLQDALPDIENIDVLIYCPGSINLKPIGNLSIDDFRTDFEINVIGAVKTIQKYL
                     PVLKKGNNPSIVLFSTVAAKLGMPFHASIATAKAGVEGLVKSLGAELASSIRINAIAP
                     TITETTLAAGILRNDRMKENMMERHPMKNYLKPKEVAEMANYLISDHAKSISGQVFEM
                     DYGIVTFKI"
     gene            complement(3624..3902)
                     /locus_tag="PLQ70_08690"
     CDS             complement(3624..3902)
                     /locus_tag="PLQ70_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016987979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HQE34729.1"
                     /translation="MKKELTDLDKDRIIEMAWEDRTTFDAILLQFGLKEQEVIELMRI
                     EMKPSSFRMWRERVQGRKTKHEKLRSFEKGKFKCNRQRQINNNKISKR"
     gene            complement(3899..5209)
                     /locus_tag="PLQ70_08695"
     CDS             complement(3899..5209)
                     /locus_tag="PLQ70_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HQE34730.1"
                     /translation="MKTIDYIPTSKIERATKLVQTGAKVGVNYLKYYGEKMVNSDLTR
                     DKLNEDNAEDIYDGLKNLKGSALKVAQMLSMDKSFLPQAYVEKFSLSQFSVPPLSAPL
                     VLKTFKNNFGKTPHEIFDEFNANSVNAASIGQVHLAVKNGKKLAVKIQYPGVANSISS
                     DLALVKPIAIRMFNLQGKDSDKYFKEVEDKLIEETNYLLELKQSQEVVKACYKIENLV
                     FPNYYPDYSSDKIITMDWMTGMHLSEFTNKNTNQEVADKIGQALWDFYMYQIHILKKV
                     HADPHPGNFLVNEKNELVALDFGCMKTIPNDFYVPYFELINKDVIDNNELFSNKLFEL
                     EILRTDDSVEEIGYFTQMFYDLLSLFTQPFQSETFDFSNEVFFQNIAQLGERFSKDTN
                     LKKMNGNRGSKHFIYMNRTFFGLYNLMFDLKAKIVVNEFKKYQD"
     gene            complement(5210..5515)
                     /locus_tag="PLQ70_08700"
     CDS             complement(5210..5515)
                     /locus_tag="PLQ70_08700"
                     /inference="COORDINATES: protein motif:HMM:NF036524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HQE34731.1"
                     /translation="MQLSSLRSEFKKYVAEIITDEERIQFEKLQNFQEKAIQESAWIQ
                     LLVTLKFWLEDESPAFEKTDIFIEKSVKLSFELMNTTPLDSLIDFGKFLFKEKTQKN"
     gene            complement(5490..>6027)
                     /locus_tag="PLQ70_08705"
     CDS             complement(5490..>6027)
                     /locus_tag="PLQ70_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HQE34732.1"
                     /translation="GALKRCQEYMDEGYRWLVDIDLALYFDTVNHDKLIGLIHKEVKD
                     IRVISLIRKYLNAGAIVNGVVTSTELGVPQGGNLSPLLSNIMLNELDKELTKRGLRFV
                     RYADDCNIYVKSKRAAERVMESITNFIEGDLKLKVNTDKSKVDRPWRCKFLGYSFYLS
                     KGRQKPTQKFNTIIKSSQ"
BASE COUNT         1783 a         1080 c         1067 g         2097 t
ORIGIN      
        1 ttcctatctt gtttttcaat ttagataaat ggtacactgc cgtaaattat agtgttttta
       61 ttggcatttt gacgctgacc gttttcaaaa taccaaatgt actgaatact tttttcatca
      121 catttctaat cattctaatt ccatttttga ttgtaaacgg aattctaacg ggtagcttta
      181 ttgacgaacc tgtggtgatt tataataatg cagaaaacct cggaatccga atcgggacaa
      241 ttcctgttga agatgtggct tatgcttttt cgatgctgtt gatgagttta attttgataa
      301 aaaaaataga aagcaattag ccatagatta cacggattga aaagattttc gccgatttta
      361 gattgtgaat tttatgttta tgattggtgt aatacaaatt gtcttattaa gataatttaa
      421 ataaaaacaa actgtgtaaa tcctttcaat ccgtgtaatc tgtggcaaaa aaataaataa
      481 atgaaagtat ataaaaaaga aaccgttcag cacatcaatg cgagtatgga agaatgttgg
      541 gcttttttct ccagtccacg aaatttgcag aaaattacgc cgactacaat gggttttaat
      601 atcaccgatt ttgacggaaa gaatatgtat gctggacaaa tcattcaata caaagtgtct
      661 ccacttttgg gtttaaaatt gcgatggaca accgaaatta ctgtggtaaa ggataacgag
      721 tatttcatcg atgaacaacg ttttggtcct tatgtgcttt ggcaccacaa acattttttt
      781 gaacccacag caaacggtgt aaaaatgacc gatgtggtgc attatgcctt gcctttggga
      841 tttattggca gaattatgaa tgcgttggtg gtcaaaaaca agctgaaaga aatctttgaa
      901 tatcgagtga aagccgtgaa cgaaattttt aattctaaat aatggctaag caagaagtaa
      961 ctcttttttg gtttcgtaga gatttgcgat tagaagataa tgtaggtttg tttcacgctt
     1021 tgcaatcgca atatccagtc attccaatat tcatttttga tgaagctatt ttggatagtt
     1081 tgccaaaaaa tgatgcccga gtaggtttca tccacgaatc actttcgaaa attaatcaaa
     1141 aattagaagt aataggaagc tcgcttttgg tcaaaaaagg taaaacgaca gccgtttggc
     1201 aacagcttat tcaagaattt gatgtcaagg aagtgttctt caataaagat tacgaacctt
     1261 atgctatcaa gcgcgatgca acaatttgca agtttttaga agataataaa ataagtactt
     1321 tctcttttaa agatcaagtc atttttgaag aaaaagaaat cacgaaagct gatggtttgc
     1381 cttatacggt ttacacgcct tataagaata aatggttgga agaataccat tctatcacac
     1441 cagttcctga atatgatgcc acgaataaat tttctaattt ccataaaaat gattttgctt
     1501 ttccatcttt aaacgaaatt ggatttgtag aaaaccctat aaaagtaatt ccacacaacc
     1561 taaaaaatgt cgccacctac cacgaaaccc gagattttcc ggccttgaac acgacgactt
     1621 atctatcgcc acatttgcgt tttggcaccg ttagcattcg aaaattagtg aattgggcgg
     1681 tcaaaaagaa ccaagttttt ttgagcgaac taatatggag agagtttttt atgcaaattc
     1741 tgtacagttt tcctaaagtg gtgaccaaca atttcaaatc ggcttacgat ggtattcaat
     1801 ggcgcaatga cgaggaagat ttcaagcgtt ggtgttcggg aactacaggt tacccaatgg
     1861 ttgatgccgg aatgcgagaa ctgaaccaaa ccggttatat gcacaaccga gttcgaatgg
     1921 tggtagcgag ttttctctgc aaacatttat tgctaccttg gcaatggggc gaagcctatt
     1981 ttgcacaaaa attattggat tatgaattgg cagctaatgt cggaaactgg caatgggcgg
     2041 caggaacagg ttgtgatgct gcgccttatt ttagggtttt caatcccgaa attcagctcc
     2101 agaaattcga tgaaaaagga atctacattc gaaaatggat tcccgaattt gatttgggtt
     2161 atgggcaacc aatggtcgaa cacgcctttg ccagagatcg ggcgattgcc acctacaagg
     2221 cggggatttt gaaatgacat tgtttttgtt tgtttgttcg tagagacgcg atgcatcgtg
     2281 tctctatgat aatttattgg taatcaaact aataaattct tttctggttt tatttttttc
     2341 gaacgcccca ttaaaagacg atgttgtcgt caccgaattt tgtttttgaa tgccacgcat
     2401 acacatacac atatgttgcg cttcaataac gacggcaacc cccaatggat ttaacgcact
     2461 ctgaatacaa tttttgattt cgtcggtaag tcgttcttga acctgcattc ttctggcaaa
     2521 agcatctaca attcttggaa ttttactcaa acctacaatc tttccgttcg ggatgtaggc
     2581 aatgtgagct ttgccaaaaa aaggcaacat atgatgttcg cacatcgagt agacttcaat
     2641 gtcttttacg acaatcattt gtttatgatt ttcggtaaaa agtgccgatt ttagtatctc
     2701 aagcggattc aacccataac cgtgtgttag atattgcatt gcttttgctg ctctttcggg
     2761 tgtttttaat aggccttcac gattgacgtc ttctcctaaa ttttctataa tagaacggta
     2821 attatctgct aaaatcgcag tggtttccgt atcatattgt tctgtttttt cgtaatttaa
     2881 tactttggtt tctatcattt tatgtttttt aaattaagga tggtttggtt tatattttaa
     2941 aggtcacgat gccataatcc atttcaaaaa cttgtcccga aatggatttg gcgtggtctg
     3001 aaatcaggta attggccatt tcggctactt ctttgggttt tagataattt ttcatcggat
     3061 ggcgttccat catattttct ttcattcgat cgttgcgaag aattcctgcg gcgagcgtag
     3121 tttcggtaat cgtcggagcg atggcattaa ttcgtataga agaagccaat tcggctccta
     3181 atgatttgac caaaccttcg actcctgctt tcgccgtagc gatactggcg tgaaaaggca
     3241 ttcccaattt cgctgctacg gtactgaaca aaacgatgga tggattgttg ccttttttca
     3301 atacaggtaa atatttttga atcgttttta cagctccaat cacattgatt tcgaaatcgg
     3361 ttctaaaatc atcgatgctc aaattgccaa tgggttttaa attaattgaa cctggacaat
     3421 aaatcaaaac gtcaatattt tcgatatcag gaagtgcatc ttgcaagaca tctaatgaat
     3481 ggtgagttaa attaggatgt gaaatttcgg gtgcatttct gctaatattt attactttgt
     3541 tcttttctaa ttgttgcagg acaatggcat ttccaattcc tttgctaccg ccaatgatga
     3601 ggatgttttt catttttttt tagctatctc ttcgaaattt tgttattgtt aatttgtctt
     3661 tgtcggttgc atttgaattt gcctttttca aaactcctta atttttcgtg ttttgttttc
     3721 cgaccttgca ctctttcgcg ccacattctg aaactcgaag gtttcatttc tatgcgcatc
     3781 aattcgatga cttcttgttc tttcaagcca aattgcagta agatggcatc gaatgttgtt
     3841 cggtcttccc aagccatttc tatgattctg tctttgtcta aatcggttaa ttcttttttc
     3901 aatcttggta ttttttaaat tcattgacca caatttttgc tttcaaatcg aacatcaaat
     3961 tgtacaatcc aaagaaggta cgattcatat aaataaaatg tttcgaccct cgattgccgt
     4021 tcattttttt gagattggta tctttcgaaa atcgttcacc taattgagca atgttttgaa
     4081 aaaacacttc gttcgaaaaa tcgaaagttt ccgattgaaa aggttgggta aaaagcgaca
     4141 ataaatcata aaacatttgg gtaaaatagc cgatttcttc gactgaatca tcggttctga
     4201 gaatttctaa ttcgaataat ttattgctaa aaagctcatt attatcaatc acatccttat
     4261 ttatcaattc aaaataagga acataaaaat catttggaat agttttcata cagccaaaat
     4321 ccaaggcaac taactcattt ttttcgttga ccaagaaatt cccaggatgt ggatcggcgt
     4381 gtactttttt caaaatatga atttgataca tatagaaatc ccataaagct tgaccaattt
     4441 tatccgcaac ttcctgattg gtgtttttat tggtgaattc cgaaagatgc attcctgtca
     4501 tccagtccat cgtgatgatt ttatcggatg aatagtcagg ataataattc ggaaaaacta
     4561 agttttctat tttatagcaa gcctttacaa cttcttgact ttgtttcaat tccaataaat
     4621 aattggtttc ttcaatcaat ttgtcttcta cttctttgaa atatttatcg gaatctttcc
     4681 cttggaggtt gaacattcga atggcaatgg gttttaccaa agccaaatcc gacgaaatgc
     4741 tattggcaac gcccggatac tgaattttta ccgccaattt tttaccattt ttcacagcca
     4801 aatgtacctg accaatactg gctgcattta cagaattggc attgaattca tcaaaaattt
     4861 catgaggagt ttttccaaaa ttatttttaa atgttttcaa gaccaaaggc gcagaaagag
     4921 gaggaaccga gaattgggat aatgaaaatt tctcgacata agcctgtggc aaaaaacttt
     4981 tgtccatact caacatttga gcaactttca acgcgcttcc tttgaggttt ttcaatccat
     5041 cataaatatc ttcggcattg tcttcgttca atttatcacg agttaagtct gaattgacca
     5101 ttttttcgcc gtaatatttc aaataattga cgcctacttt ggcaccagtt tgtaccaatt
     5161 ttgtagctct ttctattttg gaggtcggta tatagtctat agttttcatc tagttttttt
     5221 gtgtcttttc tttgaaaagg aattttccaa aatcgattag gctgtctaat ggtgtggtat
     5281 tcatcaattc gaaggataat tttactgatt tttcaataaa aatatctgtc ttttcgaaag
     5341 caggggattc atcttctaac caaaacttca aggttactaa caattgaatc caagccgatt
     5401 cctgaatggc tttttcttga aaattttgca gtttttcgaa ttgtattcgc tcttcatctg
     5461 taataatctc ggcaacatat ttcttaaact cactgcgaag acttgataat tgtattaaat
     5521 ttttgagttg gtttttgtct tccttttgat aagtaaaagg agtatcctaa gaatttacaa
     5581 cgccaaggtc tatccacttt gcttttatct gtgtttacct tcaatttaag gtcgccctca
     5641 ataaaatttg ttatgctttc cataactcgc tcagcagctc ttttgctctt tacataaata
     5701 ttgcaatcat cagcatatct aacaaatctt aaacctcttt tggtaagttc tttatcaagt
     5761 tcatttagca ttatgttgct taaaagagga gataaatttc caccttgagg gactcctaat
     5821 tctgttgatg tcacaactcc atttactatg gctcctgcgt taagatactt tcttatgagg
     5881 gatattaccc taatatcttt gacttccttg tgaatcaatc ctattaattt gtcatggttt
     5941 accgtatcaa aatataatgc caaatctata tcaaccagcc atctatagcc ttcatccata
     6001 tattcctgac atctttttaa tgcccca
//
LOCUS       DAOSNN010000164         6024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13291_length_6024_cov_4.120623, whole genome shotgun sequence.
ACCESSION   DAOSNN010000164 DAOSNN010000000
VERSION     DAOSNN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13291_length_6024_cov_4.120623"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLQ70_08710"
     CDS             <1..649
                     /locus_tag="PLQ70_08710"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HQE34733.1"
                     /translation="TWVSLNSVTKFSEHWGVAADVHLRTNDFFQDNNFFFLRGGIAYI
                     PNSKFSFTGGYAHMWLAPKNPTWSTFSDENRIYQQVQMNTKSGNVSILQRLRNEQRWQ
                     EIIVNDVETGKNKFSNRVRYLISTTIPIFKKKTAPSLVLSDELLIQFGKDIVYNTFDQ
                     NRFFVGIKQAINPKLSFDFGYMNVYQQKSTGYQYDMNHTLRLFFYLNSSVASTKK"
     gene            1106..1945
                     /locus_tag="PLQ70_08715"
     CDS             1106..1945
                     /locus_tag="PLQ70_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE34734.1"
                     /translation="MTNWIVFAQNASSEQARIIKLDEAIQLGIQNNRQLKMANTDVAI
                     ANENVSQAKIAKVPRLGLNSGYSYIGNPKIYNGFYESSTTIDYYNHQASGNIVGTVPL
                     YLGGVINSQIDQQKLISQMQESVVRMTEAEIKLNIAQQFFTLEKLYRQIETTKQNIVN
                     TELRIKQLQSRVANGQNLKSDLLRTEMQQSNFKVSVYQSSNNIKLISNYLDILIGLPA
                     DTILKPDLAGIERPTEAIDLQKSLSEAYQNRVEIKQSEIRVQLSESSLSITKSGFFTV
                     CKC"
     gene            1917..2390
                     /locus_tag="PLQ70_08720"
     CDS             1917..2390
                     /locus_tag="PLQ70_08720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE34735.1"
                     /translation="MVFLPYVNANVILNSQYPAQWPNYADNTVNFWAAGVSLNWDISS
                     FYNLKHKISSNKLAIDKSNIALEATKDRIDTDVNKAYVKYAESMEVIPTFKKDVELSQ
                     SNYKIVKSRYDNDFALISDMVDAEIQLNASKISLINANLDLMIQYYALQFAMGKL"
     gene            2404..3363
                     /locus_tag="PLQ70_08725"
     CDS             2404..3363
                     /locus_tag="PLQ70_08725"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HQE34736.1"
                     /translation="MSEETAKTAEVQQTEKKRNRILTILSFVFLLAGAIWIVSLFFDF
                     SRYETTNNAQVESYINTIAARATGHIVAIKFEANQQVRQGDTLLILDDSEYQIKVKQA
                     EADLAVAEGNLHSLEQTIITSQSNQAATKAKLEGDKASLDKAKKDYQRFKKMYADSAV
                     TQNQYEQALAKLQSSEAYIIAGENEVKASSSVALQSKTNLEASRATVARKQADLAAAQ
                     LQLSYTIITAPIDGFVGERTLSVGDLVSTNQVVASIVLKQKIWVTANFKETQVEKIKI
                     GQAVTISIDALDNKKFKGKVVGFSPATGAKFSMVEPDNSTGIL"
     gene            3471..3716
                     /locus_tag="PLQ70_08730"
     CDS             3471..3716
                     /locus_tag="PLQ70_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34737.1"
                     /translation="MVAYKKGSIFILVGLYFLTIPFFNALNVTTYDNSQILGHFGSST
                     TVFTYSSYIPVFALLAFLPLGLKIGKQIPVRILNFIG"
     gene            complement(3765..3977)
                     /locus_tag="PLQ70_08735"
     CDS             complement(3765..3977)
                     /locus_tag="PLQ70_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34738.1"
                     /translation="MDTKSPIINTSKINTNIFINVLTPLHNKLHNSIHYGHIECGIVL
                     EHNGNIVAKIPITETIANSERQKVNQ"
     gene            3999..5039
                     /locus_tag="PLQ70_08740"
     CDS             3999..5039
                     /locus_tag="PLQ70_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34739.1"
                     /translation="MGIILAWIFYRKDVAPMKGQFQFDWRGWGIMILFFAIMLFLCSE
                     GQTRNWFSDPMISYAVASLLLILGVYFLHVRFTENPIINTDVFKYKNVVIGSFLFFYT
                     GLMNGTGSVVTGFMTNVLGFDSIYVASSHLAILLGLCIAIPLSTYLLYKRIYLATIWI
                     VGFACYGMFHLLLYFRFYPGITTTDFFLPLVFKGLAIGFLYPISALYISEGVPAKLSD
                     SRMMSGIVSRVIIATLLGGAILGTFINDAKVQHKTGLSQQLSAVNQQAKKKLDNTKNY
                     YLHQGLSEVEAQKMAEKNLSNQTTQSSLLLAYKDIYLVMSAICFLPIFILLLFKLGRR
                     PIGRVDVEPIPF"
     gene            5226..5765
                     /locus_tag="PLQ70_08745"
     CDS             5226..5765
                     /locus_tag="PLQ70_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HQE34740.1"
                     /translation="MLFLMAGIFSLSSTNKQALQISIIPVLFIVFSWISVFKATPFVL
                     LTELCLSIFTFLLLIILVLKKVLEPGPINGYRIIGSIVVYMLMAHLWAVVYIFFYEHV
                     SGSFQLSLPAFDSNSLQANFLYFSYITLSTTGFGEIVSLHPFARALVNLEAIFGVLYP
                     ILLIGRLVSDANLPYKKIE"
     gene            5801..>6024
                     /locus_tag="PLQ70_08750"
     CDS             5801..>6024
                     /locus_tag="PLQ70_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34741.1"
                     /translation="MKNSSANNSKQLFETILQLGLVFLIIGFCFNLLSPFVTPILWAI
                     IFAIILYPLFNWLQKKLKGKKSLAATLIVL"
BASE COUNT         1955 a          948 c         1065 g         2056 t
ORIGIN      
        1 aacatgggtt tcactcaata gtgttaccaa attcagtgaa cattggggtg tagcagccga
       61 tgttcatctt cgaaccaatg atttttttca agataataat tttttctttt taagaggtgg
      121 tattgcgtat atacccaatt ccaaattctc ttttacaggg gggtatgctc atatgtggtt
      181 ggcaccaaaa aatcctactt ggagtacttt ttcggatgaa aatagaattt atcaacaggt
      241 tcaaatgaat actaaatctg gaaatgtgag cattctccaa agattaagaa acgaacaacg
      301 atggcaagaa ataattgtca atgatgtaga aacgggtaaa aataaattct cgaatagagt
      361 gaggtattta attagtacca ccattccaat ttttaagaaa aaaacagctc cttcgctagt
      421 tttatcggac gaattattaa ttcaattcgg aaaagatatt gtctataaca cttttgatca
      481 aaaccgattt tttgttggaa tcaaacaagc catcaatccg aaattgagtt ttgattttgg
      541 ttatatgaat gtgtatcaac aaaaatctac aggttatcaa tatgatatga atcatacctt
      601 gagattgttc ttttacttga attcgagtgt agcgtcaaca aagaaatagt tttagaaaaa
      661 aataaacttt gattttttga aataactcca aaaacctcga gtatgattat tttaattgta
      721 ctcgagtttt ttttgaactc atttttaaaa ttttaactag aatatttata attactgaac
      781 atctcattct aagattaaat agtattccaa ctaaaaagac ccttacggtt tagtataaat
      841 tatccgtgtc atctgtgtgc tattatgtgc aactgaatat tttttgacta accaaaatgt
      901 taaaaacaaa tgccttgaaa ttagattgat aattaatacg tattaaatat atatgcttat
      961 atttgttttg tattctatga atttttttaa taaaatttct aaaaaataga atgttacttc
     1021 atgtttattg ttaaaaatgt tcaatagaat tatttgcttt ctgctatgct aaaaataaaa
     1081 tcgctcacat taggaattct tttttttgac taattggatt gtatttgccc aaaatgctag
     1141 ttcagaacaa gcaagaatca taaaactcga cgaggccata caattgggta ttcaaaacaa
     1201 tcgccaactt aaaatggcga atactgatgt ggctattgcc aatgaaaatg tgagtcaggc
     1261 caaaattgct aaagttcctc gtttgggtct gaattcaggt tacagttaca ttggtaatcc
     1321 aaaaatttac aacggatttt atgaaagtag caccacaatt gattattata atcatcaagc
     1381 ctctggaaat atagttggta ctgtgccatt gtatctgggc ggagttatta atagccaaat
     1441 cgatcaacaa aaattaatca gtcaaatgca ggaatctgtg gtacgaatga cagaagccga
     1501 aatcaaatta aacattgcac agcaattttt tacactcgaa aaattgtaca gacaaataga
     1561 aaccaccaag caaaatattg ttaatacaga actacgtatc aaacagttgc agtctagagt
     1621 ggccaatggt caaaatctaa aaagtgattt gctacgaact gaaatgcagc aatccaattt
     1681 taaagtatca gtttatcaga gtagtaataa catcaagcta atcagtaact atttagatat
     1741 tttaattgga cttcctgccg atactattct aaaaccagat ttagcaggaa ttgaacggcc
     1801 taccgaagca attgatttgc aaaaaagcct atcagaagcc tatcaaaaca gagtagaaat
     1861 aaaacaatcc gaaattaggg ttcaactatc ggaatcgtca ttaagcatta ccaaaagtgg
     1921 tttttttacc gtatgtaaat gctaatgtta ttctgaattc ccaatatcct gctcaatggc
     1981 ccaactatgc tgataatact gtcaattttt gggcagctgg tgttagtctg aattgggata
     2041 tttctagttt ttataatttg aaacacaaaa ttagtagcaa taaattggca atcgacaaga
     2101 gtaacattgc attagaagcc actaaagacc gaattgatac cgatgtaaac aaagcatatg
     2161 taaaatatgc cgaaagtatg gaagtcattc ccactttcaa aaaagatgtg gaattgagcc
     2221 aaagcaatta taaaattgta aaaagtaggt acgataatga ttttgcttta atcagtgata
     2281 tggtagatgc tgagatacag ctcaatgcgt cgaagatttc gcttattaat gccaatttag
     2341 atttaatgat tcaatattat gccttgcaat ttgcaatggg taaactttag ataaaccaaa
     2401 attatgagtg aagaaacggc aaaaacagcg gaagttcaac aaaccgagaa aaagagaaat
     2461 aggattttga cgattctttc ttttgttttt ttattggcag gagcaatttg gattgtgagt
     2521 ctattttttg atttcagccg atatgaaact acgaataatg cacaggtcga atcttatatc
     2581 aataccattg ctgctcgggc aacgggacat attgtggcta tcaaattcga agccaatcaa
     2641 caagttcgtc aaggcgatac acttttgatt ctcgatgatt cagaatacca aatcaaagtc
     2701 aaacaagccg aagctgattt ggctgtagcc gaaggcaatt tgcattcatt ggaacaaact
     2761 attatcacat cacaatcaaa tcaagcggca acaaaagcta aattagaagg agacaaggca
     2821 agtcttgata aagctaaaaa agattaccaa cgatttaaaa aaatgtatgc tgattcggca
     2881 gttacccaaa atcaatacga acaagcttta gcaaaattac aatcctccga agcctatatc
     2941 attgcgggag aaaatgaagt gaaagcgagt agttcagtcg ctctgcaaag caaaaccaat
     3001 ctggaagcat caagagctac agtagccaga aaacaagccg atttagccgc tgcccaattg
     3061 caattgtctt acaccataat cactgcccca attgatggtt ttgtgggtga aagaaccctt
     3121 tcggtgggcg atcttgtcag tacaaatcag gttgtggcct ctatagttct caaacaaaaa
     3181 atatgggtaa cggccaattt caaggaaaca caggtcgaaa aaatcaaaat tggacaagct
     3241 gttactattt cgatagatgc tcttgataat aagaaattta aaggaaaagt ggttggtttt
     3301 tcgccagcaa caggagccaa gttttcgatg gtagagcccg ataattctac aggaattttg
     3361 taaaaattac acaacgcatc cctgtaaaaa tagaattcga cgccgccgcc aaggatttag
     3421 aagaagtaaa accaggaatg aatgttactg tggaagttaa aaaataaaat atggtagcat
     3481 ataaaaaagg aagcattttt attctcgtag gattgtattt tcttaccatt ccttttttca
     3541 atgctttgaa tgttacgact tacgataatt cacaaattct gggtcatttt ggatcttcaa
     3601 caaccgtgtt tacctattcg agttatattc ccgtttttgc cttgttggct tttttgccct
     3661 tgggtttaaa aataggaaag caaattcctg ttcgaatcct taattttatc ggttagtttt
     3721 ttatccattt tattcaatac agcttcctta ttttcggata gtatttattg gtttactttc
     3781 tggcgttcgc tattggcaat ggtttcggta attgggattt ttgctacgat gttgccattg
     3841 tgctcaagta caatcccaca ttcaatatgg ccataatgta tggaattatg caatttatta
     3901 tgcaagggag tcaacacatt tataaatata ttggtgttta ttttactagt atttatgatt
     3961 ggacttttag tatccatttt ctgaatttca atttttttgt gggtatcatt ttagcttgga
     4021 tattttatag aaaagatgtg gctccaatga aaggacaatt tcaatttgat tggcgaggct
     4081 ggggaattat gattttgttt tttgcgataa tgctttttct ttgctccgaa gggcaaaccc
     4141 ggaattggtt cagcgatcca atgatttctt atgcagttgc ttctttgttg ttaattttag
     4201 gtgtttattt tttgcatgta cgttttaccg aaaatccaat catcaacacc gatgttttta
     4261 aatataaaaa tgtagtaatt ggttcgtttc ttttttttta cacgggtcta atgaacggaa
     4321 caggtagtgt ggttacgggg tttatgacaa atgttctggg ttttgatagt atttatgtgg
     4381 cgagtagtca tctggcgata cttttgggtt tgtgtatcgc cattccgttg tcaacttatt
     4441 tattatacaa aagaatttat ttggccacca tttggattgt tggttttgct tgttatggaa
     4501 tgttccattt gttgctctat tttagatttt atcccggaat tacaaccacc gattttttct
     4561 tgcctttagt ttttaaagga ttagccattg gatttctgta tccgatttca gccctttata
     4621 tttccgaagg tgttcccgca aaattgagtg attctcgaat gatgagtggt attgtttctc
     4681 gggttattat tgccacattg ttgggaggcg ctattttggg aacttttatt aatgatgcta
     4741 aagtacaaca caaaacgggt ttgagccagc aattatcagc agtaaatcaa caggcgaaaa
     4801 agaaattaga caacaccaaa aattactatt tgcatcaagg attatccgaa gttgaagcgc
     4861 aaaaaatggc cgaaaagaat ctgtctaatc aaactactca atcttcgtta cttcttgctt
     4921 ataaggatat ttatttggtg atgtcggcta tttgtttttt gccaatattt attcttttgt
     4981 tattcaaatt aggacgaaga cctatcggaa gggtagatgt agagccaata cctttttaaa
     5041 attagtgatt aatatttaaa atttgataga atgaaaaaac aaaaaaagtt cgattattca
     5101 atcgtttatg gaatcaagaa agcggactta gcggtatgat attattactg accataatgc
     5161 aattcgtttt gattcctata tttggcagct acactttttt tattggaata ttgaatattt
     5221 tttggatgtt gtttctaatg gcgggtattt tttctttgtc gtcgaccaac aaacaagctc
     5281 ttcaaatttc aataattcca gtattattta ttgttttttc ttggattagt gttttcaaag
     5341 ccactccttt cgttttgctt actgaactct gtctgtctat atttactttt ttactattga
     5401 ttatattggt gttaaaaaaa gtattagaac caggaccaat aaacggttat cgaattatcg
     5461 gttcaatagt agtttatatg ctaatggctc atctttgggc agtagtttat attttctttt
     5521 atgagcatgt ttccggttct tttcagctca gtttgcctgc ttttgacagc aatagtttac
     5581 aagctaattt tttgtatttc agttatatta ccttatcgac aacaggtttt ggtgagattg
     5641 tgtcattaca tccctttgct cgtgcattgg tgaacttaga agcaattttt ggtgtattgt
     5701 atcccatatt actcattggc aggctcgtat cggatgccaa tttaccttat aaaaaaatag
     5761 aataaattct atataatttc aattcttaaa ttcaaacctt atgaaaaatt cttctgcaaa
     5821 caacagtaaa cagctttttg aaaccatctt gcaattagga cttgtatttc taattatagg
     5881 attttgtttt aatctattgt caccattcgt gacacccata ctttgggcaa taatctttgc
     5941 cattatattg tatccattat ttaattggct tcaaaaaaag ttaaaaggaa agaaatcttt
     6001 agctgcaact ttgattgttc tatg
//
LOCUS       DAOSNN010000165         6023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13295_length_6023_cov_4.290047, whole genome shotgun sequence.
ACCESSION   DAOSNN010000165 DAOSNN010000000
VERSION     DAOSNN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13295_length_6023_cov_4.290047"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLQ70_08755"
     CDS             complement(<1..226)
                     /locus_tag="PLQ70_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HQE34742.1"
                     /translation="MKVTLRQRQKANKISLYLDYYKNGKREYEYLGLYLIPEPEKGKL
                     TQAQKDENKKILSLAESVRSKRHLEIQNGMY"
     gene            complement(234..605)
                     /locus_tag="PLQ70_08760"
     CDS             complement(234..605)
                     /locus_tag="PLQ70_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQE34743.1"
                     /translation="MSSNMAIPKTCSYCGKAFIAKTTLTRYCGHTCNSRHYKQKVKED
                     KIQNSLVEQQQTMQSLPTLQTANSNSLTSKNFLSVQDAAELIGVSRWTINRMIKRGEL
                     QIHKFGRKKIIQRTQIDKLFN"
     gene            complement(824..2224)
                     /gene="mnmE"
                     /locus_tag="PLQ70_08765"
     CDS             complement(824..2224)
                     /gene="mnmE"
                     /locus_tag="PLQ70_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQE34744.1"
                     /translation="MIHHDSIVALATPSGAGAIAIIRISGEEAITIGNTVFQSIKGKD
                     LTQQKSHTLHLGHIVDGNKTLDQVLISIFKGPNSYTGENTIEISCHGSTYIQQQIIQL
                     LLRKGCRMANPGEFTLRSFLNGKMDLSQAEAVADLISSDNEASHQIAMQQMRGGFSNE
                     IAKLREELLNFASLIELELDFAEEDVEFADRTQFWELLNRIEFVLKRLIDSFAVGNVI
                     KNGIPVAIVGEPNVGKSTLLNALLNEERAIVSHIAGTTRDTIEDELVIGGISFRFIDT
                     AGIRETKDLVESIGIQKTFEKIEQAQVVLYLFESLKFKVQSSEYIIEIEKIKNQFPLK
                     PLVVVINKIDLLTTNEIVTIRQQLETLNIKLETISAKNNLGVDELKNQLLSFVNTGAL
                     RNNETIVTNTRHYDSLLKALDEIQKVKFGLETNLSSDLIALDIREALYQFGLITGQVS
                     NDELLGNIFANFCIGK"
     gene            2397..3227
                     /locus_tag="PLQ70_08770"
     CDS             2397..3227
                     /locus_tag="PLQ70_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQE34745.1"
                     /translation="MKKILFPTDFSETAENAFVHALEFAKIVNGELVLLHSFELPVYD
                     AQFFPENYGTVYDSLQLTQFDMFKDEIPKLHAIADARNLNHIKMSHRLMDGNLLYNIK
                     NAIKEENIDFIVMGTSGATGWEAFFLGTNTGNVLTAVDVPVLCIPLEAKFKKIETIGF
                     TTRYRTKDKKALKEVLKIAKKTNAKVKCLYVQTNNSDVDTSTIKEWEEDFQGQPVQFS
                     IIPSEEVQDTIADFILFKGIDILAMLTYKRNFFVELFKPSLTQKFSNKLSIPVLALHE
                     "
     gene            complement(3296..4414)
                     /gene="dnaN"
                     /locus_tag="PLQ70_08775"
     CDS             complement(3296..4414)
                     /gene="dnaN"
                     /locus_tag="PLQ70_08775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQE34746.1"
                     /translation="MKFIVSSSYLLKQLQVLGNVINSSNTLPILDNFLFELDHQTLTV
                     SASDLETTMSATLDIDSTSKGSVAVPAKLLLEILKTFPEQPLTFTVEENSTIEISSNS
                     GKYALAYAPGEEFPKSVNLDEPSVTLVPADVLATAISKTIFAAGNDDLRPVMSGVFFQ
                     FSPQGLIFVATDAHKLVKYARTDVSASEVADFIMPKKPLTILKNILGSSDAEVKIEYN
                     DSNATFSFDNYVLMCRLIDGKYPNYEAVIPKENPNKLMIDRSQFLSSVRRVAIFSNKT
                     THQIRLKIAGAELNISAEDIDYSNKAEERLTCDYQGDDMQIGYNSRFLTEMLTNLQSD
                     MIMLEMSLPNRAGILTPVDGLEEGETVTMLVMPVMLNN"
     gene            complement(4519..>6023)
                     /gene="gldG"
                     /locus_tag="PLQ70_08780"
     CDS             complement(4519..>6023)
                     /gene="gldG"
                     /locus_tag="PLQ70_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HQE34747.1"
                     /translation="IYLQGDLPAEFKRLQSETRDLFEEFQAYNKNIIFEFVDPLENED
                     ESMDNIKELYSKGLTPINITVDDKGKQSQEMVFPWAIAVYNNKEVNIPLLKNIMGAST
                     TQKVVGSVQHLEYSIADAINKITVEKQKKVAVIKGNGELQDVQIAKFLMQVRESYHIG
                     PFTLDSVAKNPTGSLEALQKYDLAIIAKPTETFTDAEKQVLDQFIINGGKTLWLVDQV
                     NMEMDSLYNDSGATLAFPRDLNLNDMFFKYGFRINPDLVKDEHGSPIKLATGEQGSAT
                     QYQEFNWKFAPQVYPISKHPIVKNLGGIKFDFASGIDTLKNGIKKTVLLQSSLYSKRI
                     GSPAEINLNIVEEETSPNHYLNTGYIPLSVLLEGSFHSMFENRVLPFQQKSFQTAGKP
                     NKIIVISDGDLIKNQLDKNFQPVELGYDQRSGNLYDNKDFLMNCVNYLLDDNGLINIR
                     SKDLDLPLLDKEKVYESYTTTQILTIGLPNLILALFGIIFTFLRKKKYAK"
BASE COUNT         1830 a         1044 c         1083 g         2066 t
ORIGIN      
        1 catacattcc gttttgaatt tctaaatgtc ttttggaacg tacactttcc gctaacgaaa
       61 ggattttttt gttttcgtct ttttgcgctt gtgtgagctt tcctttttcg ggttctggga
      121 tgaggtataa acctaaatac tcatattctc gtttaccatt tttatagtaa tccagataca
      181 agctaatttt gtttgctttc tgtctttgtc tcaatgtaac cttcataata ccatcaatta
      241 aaaagtttat ctatctgtgt gcgttgaatt attttctttc tgccaaactt gtgaatttgt
      301 agttctccac gttttatcat tctgttgatt gtccatcgac ttactccaat tagttctgct
      361 gcatcttgaa cggataaaaa gtttttggat gtaagagagt tggagtttgc agtttgcaag
      421 gttggcaaac tttgcattgt ttgctgttgc tcaactaatg agttttgaat tttgtcttcc
      481 tttacttttt gcttgtagtg tctggaatta caagtatggc cacaatatct ggttaaagtg
      541 gttttggcta taaaagcctt accacaataa gaacaggttt tcggaattgc catattgcta
      601 ctcataatat gttgcgttgt gttgcataat gtagcgaagt gtagcaatat gtagcatcat
      661 ttcgccaagt ttgttgcctt tgaatttggg caacaaaatc tatcattggg caacaataga
      721 acaacaaata taacgaataa aaggttatcg ggcaacaaat aaaagaagaa tatttgttat
      781 aaagtcaaca aaaataggta gttaacaaat aagagaatgg taattacttt ccgatacaaa
      841 aattagcaaa aatattcccc aacaattcat cgtttgaaac ctgcccagtg atcaacccaa
      901 attgatacaa agcttcacga atatccaagg caatcaagtc actagacaaa ttcgtttcca
      961 gaccaaattt tactttttgg atttcgtcca aggcttttaa cagcgaatca taatgacgag
     1021 tgttggttac gatggtttcg ttattgcgca atgcaccagt gttgacaaac gaaaggagtt
     1081 gatttttgag ttcgtcaact cctaggttgt ttttggcgga tatggtttca agtttaatgt
     1141 ttaaagtttc aagttgttgg cgtatagtca caatttcatt agttgtaagt aaatctattt
     1201 tattgatgac aacaaccaaa ggtttaagcg gaaactgatt ttttatcttc tcaatttcta
     1261 tgatatattc tgaactttga actttaaact ttaaactttc aaacaaatac aaaaccacct
     1321 gtgcttgttc tattttttcg aaggttttct ggataccaat actttctacc aagtctttag
     1381 tttctctaat tccagccgtg tcgataaaac gaaagctaat accgccaatg actaattcgt
     1441 cttcaatggt gtcgcgagtt gttcctgcaa tgtgcgaaac aatagcacgt tcttcgttca
     1501 acaaagcgtt caacaaagtc gattttccta cattgggttc gcctacaatc gctacgggaa
     1561 ttccgttttt gatgacattt ccaacagcaa acgaatcgat taaacgtttc aaaacaaatt
     1621 ctattcgatt caataattcc cagaattggg ttctatcggc aaattctaca tcttcttcgg
     1681 caaagtccaa ttcgagttcg attaaggagg caaaattcaa taattcttct cgcagtttag
     1741 caatttcgtt cgagaatcca ccacgcattt gctgcatcgc aatttggtgt gaagcttcat
     1801 tgtcggacga aattaaatcg gcaacggctt cggcttgtga taaatccatt tttccattca
     1861 gaaaggaacg aagagtaaat tcgccaggat tggccattcg gcagcctttg cgaagcaata
     1921 actgaataat ttgctgttgg atataagttg atccgtgaca ggaaatttcg attgtatttt
     1981 cgcctgtata ggagttaggt cctttgaaaa tggaaatcaa aacctgatct agtgttttgt
     2041 taccatcaac gatatgcccc aaatgcaagg tatgagattt ttgttgggtc aaatcctttc
     2101 ctttgatgga ttgaaaaacg gtatttccaa tagtaatggc ctcttcgcca gaaatcctga
     2161 tgatggcgat tgctccagct ccagaaggtg tggcaagagc aactattgaa tcgtgatgta
     2221 tcatataaat gggtctaagt gacaaaggta ctaaatcttg ataagtaaca aattttttaa
     2281 tttaatggct tgtctgtttg cttatgttat tggtcataaa tatctcataa ttagccttgt
     2341 tgattgatta tttatgctta actttaaagt gtaaattaat actaaaagtc attaaaatga
     2401 aaaagatttt gtttcccaca gatttttccg aaacggctga gaatgctttt gttcacgctt
     2461 tggagtttgc aaaaatagtg aatggcgaac ttgttttgtt gcattcgttt gaacttccag
     2521 tttatgacgc tcaatttttt cctgagaatt atggtactgt ttatgactcc cttcaattga
     2581 ctcaatttga tatgtttaaa gacgaaattc caaagcttca cgctattgcc gatgcaagga
     2641 atctgaatca tattaaaatg agccatcgat tgatggatgg taatttattg tacaatataa
     2701 aaaatgctat caaggaagaa aatattgatt ttattgtgat gggaacttct ggagctacgg
     2761 gttgggaagc ctttttctta ggaactaata ctggcaacgt tctcactgct gtagatgtac
     2821 ctgttttatg tattccttta gaagcaaaat tcaaaaaaat agaaactatt ggttttacaa
     2881 cacggtatcg aactaaagat aaaaaggcgc ttaaagaggt gttgaaaata gctaaaaaaa
     2941 caaatgcaaa agtaaaatgc ttgtatgtgc aaactaataa ttcagacgtt gatacatcaa
     3001 caataaaaga atgggaggaa gattttcaag gtcaaccagt tcagttttct atcattccaa
     3061 gtgaagaggt acaagatact attgccgatt ttattttgtt taaaggcatt gatatattgg
     3121 ctatgctgac atacaaaaga aacttttttg tagaattatt caaacccagt ttaacccaga
     3181 aattttcaaa taaattgagt attccagttt tggctttgca cgaataaaag tatagaacaa
     3241 aaaaaaacgt ccaaattcgt tttggacgtt tttagtataa aaagtgtagt taactttaat
     3301 tgtttagcat tacaggcata acgagcattg tcactgtttc gccttcttct aatccatcaa
     3361 caggagttaa gattccagct ctgtttggca acgacatttc aagcataatc atatccgatt
     3421 gtaagttggt caacatttca gtcaagaaac gagagttata cccaatttgc atatcgtctc
     3481 cttgataatc acaagtcaaa cgctcttcgg ctttgttaga ataatcaata tcttctgcag
     3541 aaatattcaa ttcggctcca gcaattttca atcgaatttg gtgtgtggtt ttgttagaga
     3601 aaatcgcaac acgacgtaca gaactcaaga attgagaacg atcaatcatc aatttgtttg
     3661 gattttcttt tgggataacg gcttcgtagt ttggatattt accatcaatc aaacggcaca
     3721 ttaaaacata attgtcgaaa gaaaaagtag cattcgaatc gttgtattcg atttttactt
     3781 ctgcgtccga agaacccaaa atatttttca aaatagtcaa aggtttcttt ggcataatga
     3841 aatcagccac ttctgaagcg cttacatccg tacgagcata ttttactaat ttgtgtgcat
     3901 cggtagctac aaatattaaa ccttgaggtg aaaattggaa gaaaacacca gacattactg
     3961 gacgtaaatc atcgtttcca gccgcaaaaa tagttttgct gatggcagtt gccaaaacat
     4021 cggcaggaac taaagttact gatggttcgt ctaagtttac agattttgga aattcttctc
     4081 ctggagcata tgccaaagca tattttcctg aattagagct gatttcaatc gtgctatttt
     4141 cttcaacagt gaaagtcaaa ggttgttcag gaaacgtttt gaggatttct aaaagtaatt
     4201 ttgcaggaac agcaacactt cctttgcttg tagaatcgat gtctaaggtg gctgacattg
     4261 tcgtttctaa atcggaagca gaaaccgtta atgtttgatg atccaattca aaaaggaaat
     4321 tgtctaaaat aggtaaagta ttgctactat tgataacatt tcctaaaacc tgtaattgtt
     4381 ttaataagta cgaactcgat actataaatt tcatctgttt tcttttattg gtatatattt
     4441 tttactgaat ttcaaaatac gatacagtct acaaatatat cgtaaactaa tttagaataa
     4501 aaactttttt tattaacatt acttagcata ttttttcttc ctcaagaacg taaatataat
     4561 gccaaaaagc gctaaaataa ggtttggaag tccgatggtt aggatttgtg tagtcgtgta
     4621 actttcgtaa actttttctt tatctaacaa aggcaaatcg agatctttgc ttcgaatgtt
     4681 aataagtccg ttgtcgtcga gtaaataatt cacacaattc atcaagaaat ctttgttgtc
     4741 atacaaattt cctgagcgtt ggtcataacc caattccaca ggttgaaagt ttttatccaa
     4801 ttggttttta atcaaatcgc cgtccgaaat tacaattatt ttattgggtt ttccagcggt
     4861 ttgaaatgat ttttgttgaa atggtaaaac cctattttcg aacatcgaat gaaacgatcc
     4921 ttcgagcaaa accgaaagcg gaatataacc cgtattcaag taatgattgg gcgaagtttc
     4981 ttcttcaaca atattcaaat tgatttctgc tggcgaacca attctcttcg aatatagaga
     5041 agattgcaat aaaaccgttt ttttgattcc gtttttcaaa gtatcgatac cgctggcaaa
     5101 atcgaacttg attccgccca agttttttac aataggatgt ttgctaatgg gatatacttg
     5161 tggagcaaat ttccaattga attcctgata ttgagtagca cttccttgtt cgccagttgc
     5221 cagttttatt gggcttccgt gttcgtcttt taccaaatct ggattaattc ggaatccgta
     5281 tttaaagaac atatcgttca aattcaaatc tcttggaaag gccaaagttg ctcccgaatc
     5341 gttatacaaa ctgtccattt ccatattcac ttgatctacc aaccacaagg tttttcctcc
     5401 attgatgatg aattggtcta atacttgttt ctcggcatct gtgaacgttt cggttggttt
     5461 tgcaataatg gccaaatcat atttttgcaa ggcttctagg cttcctgtag ggtttttagc
     5521 aactgaatct aaagtaaatg ggccaatgtg gtagctttct cgaacctgca tcaagaattt
     5581 ggcaatttgc acatcttgaa gttctccatt tcctttgata acggctactt ttttctgttt
     5641 ttctacggtg attttgttga tggcatcggc aatagaatat tctaaatgtt gcaccgaacc
     5701 cacgactttt tgggtagtcg aagcgcccat aatatttttc aataaaggaa tgttaacttc
     5761 tttgttgttg taaaccgcga ttgcccaagg aaaaaccatt tcttgcgatt gttttccttt
     5821 gtcgtcaacg gtgatgttta ttggggttag cccttttgaa tacaattctt taatgttgtc
     5881 catactttcg tcttcgtttt ctaagggatc tacgaattcg aaaataatat tcttgttata
     5941 ggcttgaaat tcttcgaata aatctcgagt ttcgctttgc aaacgcttga attcagcagg
     6001 taaatcgcct tgcaaataga ttt
//
LOCUS       DAOSNN010000166         5975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13424_length_5975_cov_3.806926, whole genome shotgun sequence.
ACCESSION   DAOSNN010000166 DAOSNN010000000
VERSION     DAOSNN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13424_length_5975_cov_3.806926"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PLQ70_08785"
     CDS             complement(<1..857)
                     /locus_tag="PLQ70_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HQE34748.1"
                     /translation="MKKHQFIFVCSLIFTLLFYDQSVGLNMSIFGLVLTGFICYFFQD
                     RFTSRTHLVLVVTSVLSCISFAWYGDFVSFLALALSVCFLQFKTQEKELKILQVFPIA
                     FINGITSLGRMLMFSQWLPKREVKSNFAKKLIAYFVIPTVFVTLFFTVYAFGSDHFSS
                     LFTDYTLDLNIWQLILITLLGFYISFTFWNYWVPEVFYEKNSLLANDFQDKNAIRNDR
                     TFSFLDIDFERKSGEITLFLLNVLILVFIVTYNYEQFFEVVEKSKLSAATHERVNAVI
                     FSIVMAVGV"
     gene            complement(913..1206)
                     /locus_tag="PLQ70_08790"
     CDS             complement(913..1206)
                     /locus_tag="PLQ70_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007649617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HQE34749.1"
                     /translation="MGIIDKLNKDFESRVRLGIMSILMVNDWVDFTEMKALLNITDGN
                     LASHSSALEKGDYIEIKKEFVGKKPKTSYRVTNLGRVSFVSHLNSLEKLFKTK"
     gene            complement(1216..1830)
                     /locus_tag="PLQ70_08795"
     CDS             complement(1216..1830)
                     /locus_tag="PLQ70_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34750.1"
                     /translation="MNQKHQEDLSHIRSMMERSSRFISLSGLSGVFAGLSALIGGLYV
                     YLLFKANGLDYFDGEHKIYSGNLVSELVWIALIILFFALTFGIFFTIRKSRKYNLPIW
                     TSATKNMLFNLAIPLSVGGVFCLALLYHQIYVLIAPATLLFYGLALVNAEKYTFSDIK
                     YLGFCELILGCISLFFLGYGLLFWIMGFGVLHILYGILVFKKYK"
     gene            complement(1972..2853)
                     /locus_tag="PLQ70_08800"
     CDS             complement(1972..2853)
                     /locus_tag="PLQ70_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HQE34751.1"
                     /translation="MQLLDGKKTSEDIKNEIAAEVQRMKADGQKVPHLAAVIVGTNGA
                     SLTYVGSKVKSCQQIGFESTLVALPENISEEELLDKIKELNEDDDLDGYIVQLPLPKH
                     IDEQKILMAIDPDKDVDGFHPANFGKMALDMETFLPATPFGIMELLERYKVETAGKHT
                     VVIGRSHIVGRPMSILLSRKGYPGDSTVTLTHSRTKNLEEFTKNADIIVTALGVPEFL
                     KGDMVKEGVVIVDVGITRVEDASNPKGYAIKGDVDFASVSPKSSFITPVPGGVGPMTI
                     AMLLKNTLLARKIRSRK"
     gene            complement(2981..4339)
                     /gene="ffh"
                     /locus_tag="PLQ70_08805"
     CDS             complement(2981..4339)
                     /gene="ffh"
                     /locus_tag="PLQ70_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQE34752.1"
                     /translation="MFDNLSDKLDKAFHILKGHGKITEVNVAETLKEVRRALLDADVN
                     FKIAKDFTTKVKDKAIGQNVLTTLQPGQLLVKLVKDELTELMGGDVSGINLSGNPTVI
                     LMSGLQGSGKTTFSGKLANYLKTKKNKKPLLVACDIYRPAAIKQLHVVGESLGIDVYS
                     EPDNTNPVIIAQNAISFAKHQGLNVVIIDTAGRLAVDEAMMTEISNVHQAIKPHETLF
                     VVDSMTGQDAVNTAKAFNDILNFDGVVLTKLDGDTRGGAAISIKTVVNKPIKFVGTGE
                     KMDAIDVFYPERMAERILGMGDVVSLVEKAQEHYDEEEARKIQKKIAKNEFGFDDFLS
                     QIQQVKKMGNMKDLVGMIPGASKAMKDVEIEDDAFKHIEAIIHSMTPIERSKPAIIDV
                     KRKARIAKGSGTKIEQVNQLMKQFDQMSKMMKMMQGPGGKNMMKMMGNMKGMPGGMPG
                     MR"
     gene            complement(4481..5515)
                     /locus_tag="PLQ70_08810"
     CDS             complement(4481..5515)
                     /locus_tag="PLQ70_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34753.1"
                     /translation="MLSFKKQLLYLSVVSLFLLFSCQSEIEEQVYNTQETITKSSPIT
                     SYLQRVAMVQTIQDNIIDKSSYCTIKLPYKVTVNYATISINTAADYQKVLDNINAYTN
                     DNDIVKITFPVTMIYYNYVEKVITNQTEFESLLSYWSKYPDLLSKINCVNINYPITID
                     IYNSANQIASSVKIESDSSFFSFINNLNESQFIALKYPISISDNNSVPISIANNSQFE
                     NAIKHAIDTCPDNVNANLNFIQILTTNTWKISYYSHESGKTSDYDGYTFVFNSYYKVI
                     ATKSGVTYNGTWSSKLDNGVREFKIKLDAPVLEKLDEDWKVFEFNNSQLRFRDIGSNN
                     STSYLYLEKI"
     gene            5804..>5975
                     /locus_tag="PLQ70_08815"
     CDS             5804..>5975
                     /locus_tag="PLQ70_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HQE34754.1"
                     /translation="MSNSLKSNTCDEIVFSSFFKTHAKSLRNFLFYKFGNKDQAEDLT
                     QEAFIKLWQNCTS"
BASE COUNT         2021 a         1100 c          856 g         1998 t
ORIGIN      
        1 attacaccaa cagccataac aatagagaaa atcactgcat ttactctctc gtgagtagca
       61 gcacttaatt ttgatttttc gactacttca aaaaattgct cgtaattata agtcacgata
      121 aaaaccaaaa tcaaaacatt taaaagaaac aatgtgatct ccccgctttt tcgttcgaaa
      181 tcaatatcta agaacgaaaa agtcctatca ttcctgattg catttttatc ttgaaaatca
      241 ttagctaaca gcgaattctt ttcataaaag acctctggaa cccaataatt ccaaaaagta
      301 aacgagatat aaaatcccaa aagagtaatt aaaatgagct gccaaatatt caaatctaaa
      361 gtgtaatctg taaatagaga agaaaaatga tcacttccga aggcgtaaac tgtaaaaaac
      421 aaagtcacaa aaaccgttgg aataacgaaa taagcgatta gtttcttggc aaaattgctt
      481 ttaacttctc gtttcggaag ccattggcta aacatcagca tacgacctaa agatgtgatt
      541 ccgtttataa aagcaatcgg aaaaacttgc agtattttaa gttctttttc ttgcgttttg
      601 aattgtaaaa aacagactga caaggctaaa gccaaaaaag acacgaaatc accataccaa
      661 gcaaatgaaa tacaagaaag tactgaggtc accacaagaa ccaaatgcgt ccgactcgtg
      721 aatcggtctt gaaaaaaata gcagatgaat cccgttagga ctaatccaaa aatagacata
      781 ttcagtccta ctgattgatc gtaaaaaagc agtgtaaaaa tcaaactgca cacaaaaatg
      841 aattgatgtt ttttcataat ttaaaattta aaagtacttt gcatttcaaa gcgaaaggtt
      901 aaaaaaaaag ggttatttag ttttgaataa tttttctaag ctgttgagat gtgagacaaa
      961 tgaaactctt cctaaattag tcacacggta agatgttttg ggctttttgc ccacaaattc
     1021 ttttttaatt tcgatataat cgcccttctc caatgccgaa gaatgactcg ctaaattgcc
     1081 atcagtaata ttcaatagtg ctttcatttc ggtaaaatca acccaatcat tgaccatcag
     1141 aatagacata atacctaatc gcacacggct ttcaaaatct ttatttagtt tatctataat
     1201 tcccattcgg atggattatt tatatttttt gaaaactagt attccgtaca aaatatgcaa
     1261 aacaccaaat cccataatcc aaaatagcaa accgtagcct aagaagaaaa gcgaaataca
     1321 tcctaatatt aattcgcaaa atcccaaata tttaatatct gaaaaagtat atttctcagc
     1381 attaaccaat gctaaaccat aaaacaacaa agtagctgga gcgatcaaaa catagatttg
     1441 atggtacaaa agtgccaaac aaaacacacc accaacactt aaaggaatag ccaaattaaa
     1501 tagcatgttc ttcgtagccg aagtccaaat cggcaaattg tactttctac tttttcgaat
     1561 agtaaaaaag attccgaagg tcaacgcaaa aaataaaatt atcaaagcta tccaaaccaa
     1621 ttctgaaacc aaattgccag aatagatttt gtgctcaccg tcaaaataat caagtccatt
     1681 ggctttaaac aatagataca catacaatcc gccaatcaag gcagataaac ctgcaaatac
     1741 tcctgagagt ccgcttaatg aaataaatct ggaagaacgt tccatcattg aacgaatgtg
     1801 tgataaatct tcttggtgct tctgattcat atttaaagta ctttgtactg caaagttaat
     1861 ttatattttg aattgacaaa caaataatta aaaaaaatta tggaaaagtt ttaaaacaac
     1921 aaagcctatt cggttgaata ggcttgttta ttggcttaaa gtaaaaataa tttattttct
     1981 acttctaatt tttctagcta aaagtgtatt tttcaacaac attgcaatgg tcattggtcc
     2041 tactccaccc ggaactggcg tgatgaaaga cgactttgga ctcacgcttg caaaatcaac
     2101 atccccttta atcgcgtagc cttttggatt ggaagcatcc tcaacacgag taattcccac
     2161 gtcaacaata accacaccct ctttgaccat atctcctttt aaaaattcag gaactcccaa
     2221 tgctgtaaca atgatgtctg catttttggt aaattcttcc aagttttttg tacggctatg
     2281 agtcaaagta acggttgaat ctccaggata tcctttgcga ctcaacaaaa tactcattgg
     2341 tcgacctaca atgtgactgc gcccaataac cacggtatgt tttccagcag tttctacttt
     2401 ataacgctct aacaattcca ttatcccaaa tggagtggct ggtaaaaacg tttccatatc
     2461 caaagccatt tttccaaaat tggcaggatg aaaaccatct acatctttgt caggatcaat
     2521 agccattagt attttttgct catcgatgtg tttgggtaaa ggcaattgaa cgatataacc
     2581 atctaaatca tcatcttcgt tgagttcttt gattttatct aacaattcct cttcagaaat
     2641 attttctggt aaagccacca aggtagattc aaaaccaatt tgctgacacg attttacttt
     2701 gctccccaca taagtcaaac tggcaccgtt tgtacccaca ataactgctg ctaaatgagg
     2761 tactttttgc ccatcggcct tcattctttg tacttcggca gcgatttcgt ttttaatatc
     2821 ttccgatgtt ttttttccgt ctagtagttg cattttttat attatttaaa agtttaaaaa
     2881 aagtttaaaa gtagtttaga agtttaaaaa atagtttaaa agtttaagag ttaccctcta
     2941 accttttaaa ctattaaacc ttaaactttt aaaccttttt ttatctcatt cctggcattc
     3001 cacctggcat tcccttcata ttccccatca ttttcatcat attctttccg cctgggcctt
     3061 gcatcatttt catcattttg ctcatttgat caaactgttt catcaactga ttcacttgtt
     3121 cgatttttgt tcctgaacct ttggcaattc tagcttttct tttaacgtca ataatagcag
     3181 gttttgatct ttcgattgga gtcatcgaat ggataatagc ttcaatatgt ttgaaggcat
     3241 cgtcttcaat ttctacatct ttcatcgctt ttgaagctcc tggtatcatt ccaaccaagt
     3301 ctttcatatt ccccattttc tttacttgct gaatttggga aaggaaatca tcaaaaccaa
     3361 attcattttt ggcgattttc ttttggattt ttctggcttc ttcttcgtca taatgctctt
     3421 gtgctttttc aaccaaagac accacatctc ccattccgag aatacgttca gccatacgtt
     3481 ctggatagaa cacgtcgatg gcatccattt tttcgccagt tcctacgaat ttaattggtt
     3541 tattgacaac tgttttaatc gaaatggcag ctccaccacg agtatcacca tctaatttgg
     3601 ttaaaacaac tccgtcaaaa tttagaatat cattgaaggc ttttgcagta tttactgcat
     3661 cttgtcctgt catcgaatca actacaaaca aagtttcgtg aggtttgatc gcttgatgaa
     3721 cattcgaaat ttcggtcatc atcgcctcat caacagccaa acgacctgca gtatcgataa
     3781 tcacaacatt caatccttga tgttttgcaa aactgatggc gttttgggca ataatcactg
     3841 gatttgtatt atcgggttct gaatatacat caattcctaa tgactctcca actacgtgca
     3901 attgttttat agccgctgga cgataaatat cacaagccac caaaagtggt tttttatttt
     3961 tcttagtttt aagataatta gctaattttc cagagaaggt agtttttcca gaaccttgca
     4021 aacctgacat taagataacg gttggatttc cagaaagatt gattccagac acatctccgc
     4081 ccattaattc agttagttcg tctttgacta actttaccaa taattgtccc ggttgaaggg
     4141 ttgtcaatac gttttgacca atggctttat cctttacttt ggtagtaaaa tctttggcaa
     4201 tcttaaagtt gacatcggcg tccaataaag cacgacgaac ttcttttagg gtttcggcaa
     4261 cgtttacttc ggttattttt ccgtgccctt ttagtatatg aaaggctttg tctaatttat
     4321 cgcttaaatt atcgaacata atggtaattg tcttttattg aggtgcaaat ataaactaaa
     4381 gttataaagt cacaaagtta ccaacttgta aaatacagcg tatcttaaaa aaaataaacc
     4441 agtcaataga ctggctttat tcttcaattt atttgttaga ctatattttt tctaaataaa
     4501 gataactagt actattattg ctaccaatat ctctaaaacg caattgtgaa ttattaaatt
     4561 cgaatacttt ccaatcttcg tccagttttt ctaatacagg tgcatccaat tttattttaa
     4621 attcccgaac cccattatct aattttgatg accaagtacc attataggta actcccgatt
     4681 ttgtggcaat aactttatag taagaattaa aaacaaaagt atagccatca taatccgagg
     4741 tttttccgct ttcgtgagaa taataagaaa ttttccaagt attggttgta agtatttgaa
     4801 taaaattcaa attagcattt acattatctg gacaagtatc aatagcgtgt ttgattgcat
     4861 tttcaaattg tgaattgttt gcaatagaga ttggaacact attattatca gatattgaaa
     4921 taggatattt taaggctata aactggcttt cgtttaaatt atttataaaa ctaaaaaaag
     4981 aggaatcgct ttctattttt actgaacttg caatttgatt agcactatta taaatatcaa
     5041 ttgttatcgg ataattaatg tttacgcaat taattttaga caataaatca gggtattttg
     5101 accaataaga caacaatgat tcaaattcag tttgatttgt aataactttt tcaacataat
     5161 tatagtaaat cattgttaca ggaaaagtta ttttgacaat atcattatca ttcgtatagg
     5221 cattgatgtt gtccaatact ttttgataat cggcagcggt gtttattgaa atagttgcgt
     5281 aattaactgt aactttataa ggcaatttta tggtacaata actcgatttg tcaataatat
     5341 tatcttgaat agtttgcacc atcgcaactc tttgcaggta agacgtaatt ggagaagatt
     5401 ttgtaattgt ttcctgtgta ttataaacct gttcttctat ttcactttga caagagaaga
     5461 gaagaaacaa actgactaca gacaaataga gtaactgttt tttaaaactt aacatggtag
     5521 ttttttaatc aaatgtaata aaattcaaat ttaaagattt ctttttacaa tttatgcatt
     5581 tgttataaat gcaatattca ttcaacaact tcaaaaataa gttcgttatt tttaacccaa
     5641 aacattaatt ttctaatcaa aaatattgtg tcaaaaaact gattttaaat tttctaagca
     5701 aacattaagc ttggtcagac ctctataaaa caatttagtt tacttttacc ctacttattg
     5761 aaaaataaat atatttgcaa agcccaaact gtattttata aaaatgtcaa actccttaaa
     5821 atcaaatact tgtgatgaaa ttgttttttc atcttttttt aaaacccatg caaaatcttt
     5881 gcgaaatttc cttttttaca aatttggcaa caaggatcaa gccgaagatt taacccaaga
     5941 agcatttata aaactttggc aaaactgcac atctg
//
LOCUS       DAOSNN010000167         5922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13588_length_5922_cov_6.770240, whole genome shotgun sequence.
ACCESSION   DAOSNN010000167 DAOSNN010000000
VERSION     DAOSNN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13588_length_5922_cov_6.770240"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PLQ70_08820"
     CDS             <1..249
                     /locus_tag="PLQ70_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HQE34755.1"
                     /translation="IVPKPKKFMWQFDNEGNLKSFANQQQKFLSNLMLRRGILTAEDT
                     IGQSIPLQLTGNAHSLLNLKVQDSVPADSLAVETPFEF"
     gene            complement(383..1420)
                     /locus_tag="PLQ70_08825"
     CDS             complement(383..1420)
                     /locus_tag="PLQ70_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQE34756.1"
                     /translation="MKYTTLPNTDIKISKTCLGTMTFGQQNTEAEGHAQMDYALEKGI
                     NFFDTAEMYSVPGRKETYGSTEKIIGTWFKKTGKRDQIILASKIAGPNPGLAYIRENM
                     DFSPESIALSIDKSLTRLQTDYIDLYQLHWPERKANFFGQRGFKVQNDAWEDNIHSVL
                     ETLESFIKQGKINHIGLSNETPWGIMRFLQESKYNNLPRIKTVQNPYSLLNRSFEVGN
                     AEVCWRENVGLLAYSPMGFGVLSGKFLSGESHPNARIKLFPQFARYNSAQSAEATRLY
                     NEVAQKNGLSLTELSLAFVNQQAFVTSTIIGATTMEQLKENIAAIDVVLSDEILKEID
                     AVQAVIPDPAP"
     gene            complement(1503..2444)
                     /locus_tag="PLQ70_08830"
     CDS             complement(1503..2444)
                     /locus_tag="PLQ70_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQE34757.1"
                     /translation="MIKRISLGLLILALSTSCVSKKIYNDLESKYADLKKENKSISEE
                     NEELKKAKSQLELDQAATKKELEKVKAERDKVQADYAALNKKMDVLKDSYAALEKNSD
                     ESLKANMAKNRQLLEQLAAKEKALALEQEKLDKNAKRLQELEDLIAAKEASMRKLKET
                     LSKALNGFEGKGLTVEQKNGKVYVSMENKLLFNSGSWAVGSEGKKAVVEVGKVLGDNP
                     DISVLIEGHTDDVPYGGSGPIADNWDLSTKRATAIVGILSENKAVNKQNLTAAGRGEF
                     SPLASNATPEGKAKNRRIEIILTPRLDEISKMLNDIN"
     gene            complement(2491..3255)
                     /locus_tag="PLQ70_08835"
     CDS             complement(2491..3255)
                     /locus_tag="PLQ70_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQE34758.1"
                     /translation="MKIISYNVNGIRSAISKGFIEWLQQANPDVICLQEIKATPEQIP
                     LLDFELAGYPYHYWFPATKKGYSGVAILSKIKPNNVVFGTGIEHMDFEGRNIRVDFDD
                     LSVMSLYLPSGTNSERLEHKFMYMDDFQNYINELKKEVPNLVICGDYNICHEAIDIHD
                     PIRNKKTSGFLPEERAWLDGFMKSGFIDSFRHLNKEPNNYTWWTMRFPSARLENKGWR
                     IDYCLVSEPLKQKIKRAVILPEAKHSDHCPSLVEIE"
     gene            3373..5175
                     /locus_tag="PLQ70_08840"
     CDS             3373..5175
                     /locus_tag="PLQ70_08840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HQE34759.1"
                     /translation="MGLVTAKEVAKAINTDKYGFLGTFSGWLLMKVLKISTLNKIYDR
                     NKHLSEVEFLNAILDEFQIKFEIPEEDFKRLPKDGAYITISNHPLGGIDGILLLKLML
                     EKEPNFKIIANFLLHRIDPMKPYIMPVNPFENHKDAKSSVIGIKETLRHLRDGKPLGM
                     FPAGEVSTYRDGKLVVDKEWEEGAIKVIRKAQVPVVPIYFHAKNSGLFYFLSKISDTL
                     RTAKLPSELLTQKNRVIKVRIGKPISVSEQNELTSIDEYSEFLRRKTYMLAKPFEKET
                     PFLPKPNLKLPKNPKKIATAVNQDKMITEVNSLREKDCRLLESKNYEVFFAKAEEMPN
                     ILQEIGRLREITFREVGEGTNESTDIDSYDTHYRHLFLWDGDAKKIAGAYRMGLGSEI
                     YPKYGIEGFYLTDLFGFEPELHDMLHHSIEMGRAFIIKEYQQKPMPLFLLWKGIIHIT
                     LRHPEHKFLLGGVSISNQFSNFSKSLMIEFMKSNFYDPYIAQYIHPKKEFKVKLKDAD
                     KDFIFDEAEADLNKFDKLIDELEPGSLRLPVLIKKYIKQNARVIAFNVDPLFNNAIDG
                     LMYIRISEIPDSTVKPVMEEFQAELERKLAEKEE"
     gene            complement(5203..5667)
                     /locus_tag="PLQ70_08845"
     CDS             complement(5203..5667)
                     /locus_tag="PLQ70_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HQE34760.1"
                     /translation="MTSINYTINTKFKRKIYGIIGAFITLMVPFFLIVFHQDNHLETA
                     QSLCPLKMLTGFPCPGCGITKSLVYLYEGDLQKSLYYHILGPFVILFCVATIGVLSAE
                     LITKKEYFTQLLFNKKLAYGLALFLATYHFIRIIYFIKNHSIDDIICQSIWR"
     gene            complement(5642..>5922)
                     /locus_tag="PLQ70_08850"
     CDS             complement(5642..>5922)
                     /locus_tag="PLQ70_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34761.1"
                     /translation="LYHIYWNIKVAEVLNAVTGREVISSPIAIFAGCCFPINVYFFYL
                     AGREGLPEVYKRIGYPQKDDSTLLMVLGFFFPMAAAMIVQNDINKLYN"
BASE COUNT         1860 a         1113 c         1044 g         1905 t
ORIGIN      
        1 attgttccaa aaccaaagaa atttatgtgg caatttgaca acgaaggcaa cttaaaatct
       61 tttgctaacc aacagcaaaa attcctaagc aatttgatgt tgcgaagagg aattctgacc
      121 gccgaagaca ccatcggaca atctatcccg ttgcaactta caggaaatgc ccattcgctt
      181 ttgaatttaa aagtacaaga ttctgttcca gcagattctt tggcggtaga gacacctttt
      241 gaattttgat tctttgcttt tttggacaca gatgacacag attgaaacgg atttacacag
      301 atagaaatta aaaaagtcca caaagaaaaa taattttctt tgtggacttt gtgccttctt
      361 tgtgcccttt gtgtttaaaa aactaaggcg caggatcagg aatcacggcc tgaaccgcat
      421 cgatttcctt taagatttca tccgataaca caacatcaat tgctgcaatg ttttctttta
      481 attgttccat cgttgtcgcg ccaataatgg tgctggtcac aaaagcttgt tggtttacaa
      541 atgccaaaga caattcagtt aaactcaagc catttttctg ggcaacttca ttatacaatc
      601 tggtagcttc ggcagattgt gcgctgttgt atcgggcaaa ttgtggaaat aatttgattc
      661 tcgcattcgg atgactttcg ccagacaaaa attttccaga caaaactcca aaacccattg
      721 gcgaataagc caacaatccc acattttccc tccaacaaac ttcggcatta cctacttcga
      781 aagatcggtt taacaacgaa tacggatttt gaacggtttt aattctaggt aaattattgt
      841 atttgctttc ttgcaaaaaa cgcataatcc cccaaggtgt ttcgttcgaa agtccgatgt
      901 ggttgatttt tccttgtttg ataaaacttt ccaaggtttc taaaaccgaa tgaatattat
      961 cttcccaagc atcgttttgc actttaaaac cacgctgtcc aaagaaattg gctttgcgtt
     1021 ctggccaatg caattggtac aaatcgatat aatcggtttg caagcgagtc aaacttttgt
     1081 caatggacaa ggcaatactt tctggagaaa aatccatatt ttctcgaatg taagccaagc
     1141 caggattggg tccagcaatt ttagaagcta aaattatttg gtctctttta cctgtttttt
     1201 tgaaccaagt tccaatgatt ttctcggtgc ttccgtaggt ttccttgcgt cccggaacgg
     1261 agtacatttc agccgtatca aaaaaattga ttcctttttc gagcgcataa tccatttgag
     1321 cgtgaccctc agcttcggta ttttgttgac caaaagtcat tgtaccaagg caagttttgc
     1381 taatttttat gtcggtattg ggtaaagtag tgtatttcat aagtcttaaa attataaagc
     1441 cgaaagccat aaagctaaag agggttcttt aaagctttat gactttcaac tttagacaaa
     1501 aattaattaa tatcattcaa cattttagaa atctcatcta atcttggagt caaaataatt
     1561 tcgattctac ggtttttggc ttttccttct ggagtggcat tgctggctaa tggtgagaat
     1621 tcacctcttc cggcagccgt taaattctgc ttgttgactg ctttgttttc gcttaaaata
     1681 ccaacaattg cggtagctct tttggtcgat aaatcccagt tatcggcaat gggtcctgaa
     1741 cctccataag gaacatcatc ggtatgacct tcaatcaaaa ctgaaatatc tggattgtcg
     1801 cccaatactt ttccgacctc aacaacggct ttttttcctt cagatcctac agcccaactt
     1861 cctgaattaa acaagagttt gttttccata gaaacatata cttttccgtt tttttgctcg
     1921 actgtcaatc cttttccttc aaatccattc aaagctttcg aaagggtttc ttttagtttt
     1981 cgcatactcg cttctttggc agcaatcaaa tcttctaatt cttgcaaacg tttcgcattt
     2041 ttatctaatt tttcttgttc caatgccaat gctttttctt tggcagccaa ttgttctaat
     2101 aattgacgat ttttggccat attggctttc aaagattcat cgctgttttt ttccaaagcg
     2161 gcgtacgaat ctttcaaaac atccattttt ttgtttaaag cggcataatc ggcttgcact
     2221 ttatcgcgtt ctgcttttac tttttctaat tctttttttg tggctgcttg atccaattcg
     2281 agttggcttt ttgctttttt cagctcttca ttttcttcag aaatactttt attttctttc
     2341 ttcaaatcgg catatttgct ttctaaatca ttgtatattt tcttggaaac acaagaagta
     2401 gaaagtgcta gaatcagcaa tccgagggaa attcttttta tcatttttac tgttttttta
     2461 ttgaaattga atttgacttt tgaatttgat ttactctatc tctaccaaac tcggacaatg
     2521 gtcagaatgc ttggcttcgg gtaatataac ggctctttta attttttgtt ttaaaggttc
     2581 gctaactaaa caataatcga tgcgccaacc tttgttctcc aaccttgccg atggaaatcg
     2641 catcgtccac caagtgtaat tattgggttc tttgttcaaa tgtcggaaac tgtcgatgaa
     2701 tccacttttc ataaaaccgt ctaaccaagc tctttcttcg ggcaaaaatc ctgaagtttt
     2761 cttgtttcga atgggatcat gaatgtcgat tgcttcgtga caaatgttat aatcgccaca
     2821 aatcacaagg ttaggaactt cttttttcag ttcgttgatg taattctgaa aatcgtccat
     2881 atacataaac ttgtgttcca gtctttcgct attggttcca gaaggcaaat acaaactcat
     2941 taccgaaagg tcatcaaaat caactcggat atttcttccc tcgaaatcca tatgctcgat
     3001 tccagttcca aaaactacgt tgttgggttt gattttcgac aaaatggcca ctccgctgta
     3061 gccttttttg gtagcgggaa accagtaatg atagggatag ccggcaagtt caaaatctaa
     3121 aagtggaatt tgctctggtg tggcttttat ttcctgaagg caaattacat cgggattggc
     3181 ttgttgcaac cattcgataa aacctttgga gattgcagat cgaatgccgt tgacattata
     3241 agaaatgatt ttcattgcgt ttttatttgt gattcaaaag taatgaaaac aataaagatt
     3301 gatgccaaaa atagatagaa aatagagttt tttgctatct ttgtttcttg ctcaaaaaaa
     3361 catacgctac ctatgggttt agtaaccgca aaagaagttg caaaagcaat aaacacagat
     3421 aaatatggat ttttaggtac tttttcggga tggttgctga tgaaagtctt gaaaatttcg
     3481 accctaaata aaatttacga cagaaacaaa cacttatcag aagtggagtt tttgaatgcc
     3541 atattggatg agtttcaaat aaaatttgaa attccagaag aagattttaa aagattacca
     3601 aaagatgggg cttacatcac tatttctaac catccgcttg gaggaattga cggcatttta
     3661 ctcttgaaat taatgttgga aaaagagcct aattttaaaa ttatagccaa tttccttttg
     3721 catcgcatcg atccgatgaa gccttacatc atgcctgtaa atcctttcga aaatcacaaa
     3781 gatgccaagt cgagtgtgat tggaattaaa gaaaccttac gccatttgag agacgggaaa
     3841 ccactaggaa tgtttccggc tggcgaagtt tcgacctaca gagatggtaa acttgtggtt
     3901 gacaaagaat gggaagaggg agctataaaa gtaattagaa aagctcaagt tcctgttgtt
     3961 cctatctatt ttcatgctaa aaatagtgga ttgttttact ttctttctaa aataagcgac
     4021 actttgcgaa ctgccaaatt accttccgaa ttattgactc aaaaaaatag agtaatcaaa
     4081 gttagaattg gaaaacctat ttcagtatca gaacaaaacg agctcacttc aatagatgag
     4141 tattcagagt ttttaagacg aaaaacctat atgttggcca aacctttcga aaaggaaaca
     4201 ccgtttttgc caaaacccaa tttaaaacta cccaagaatc caaaaaaaat agctactgct
     4261 gttaatcaag acaaaatgat tacagaagta aactccctaa gagaaaaaga ttgtcgatta
     4321 ttggaaagca aaaactatga agtcttcttt gccaaagccg aagaaatgcc aaatatcctt
     4381 caggaaatag gtcgattacg agaaattact ttcagagaag tgggcgaagg aaccaatgaa
     4441 tctaccgata tcgattccta cgacacccac tatcgtcatt tgtttttatg ggatggcgat
     4501 gccaaaaaaa ttgctggtgc ctatcgaatg gggctgggtt ctgaaattta tccaaaatat
     4561 ggaattgaag ggttttattt aacagatttg tttgggtttg aacccgaatt acacgacatg
     4621 cttcaccatt ctatcgaaat gggtcgtgcc tttatcatca aagaatacca acaaaaaccg
     4681 atgcctcttt tcttgctttg gaaaggaatt attcacatta cgctgcgtca tcctgaacat
     4741 aaattcttgc ttggaggcgt gagtatcagc aatcaatttt cgaatttttc gaaatcgctg
     4801 atgattgaat ttatgaagtc taatttttat gacccctata ttgctcaata cattcatcct
     4861 aaaaaggagt ttaaagtcaa gctaaaagat gccgataaag attttatttt tgatgaagcc
     4921 gaagccgatt tgaacaaatt tgacaaattg attgacgagt tagagccagg ttctttgcgt
     4981 ttgcccgttt tgatcaaaaa atacatcaaa caaaatgcac gtgtcattgc ttttaatgtt
     5041 gacccactat tcaataacgc tattgacgga ttgatgtaca ttcgaatttc tgaaattcca
     5101 gatagtactg taaaacctgt tatggaagaa tttcaagcag aattagaaag aaaattagca
     5161 gagaaggaag agtaatcaga tggcagaagg cggcttaact ctttacctcc aaatagactg
     5221 acatataata tcgtcaatcg aatgattctt gatgaaataa ataattctaa taaaatgata
     5281 cgttgctaga aaaagagcca atccataggc tagtttttta ttgaacaaca actgtgtaaa
     5341 atattctttt ttagtaatca attcggcaga aagtacacca attgttgcca cacaaaaaag
     5401 aatcacaaaa gggcctaaaa tatgataata caaacttttt tgtaaatcgc cctcatataa
     5461 ataaaccaac gattttgtga tgccgcagcc cggacacgga aaacctgtaa gcatcttcaa
     5521 agggcaaagc gattgagccg tttcgagatg attgtcctga tgaaaaacaa ttaaaaaaaa
     5581 cggaaccatc agcgtgataa aggcgccgat gattccgtag attttacgtt taaattttgt
     5641 attaattgta taatttattg atgtcatttt gaacaatcat tgctgctgcc attggaaaga
     5701 aaaaccctaa aaccatcaat aatgttgaat cgtctttttg tggataacca atgcgtttat
     5761 aaacttcagg caaaccttct cttcctgcta aataaaagaa atagacattg ataggaaaac
     5821 aacatcctgc aaaaatagca atcggcgacg aaataacttc acgtccggtg actgcgttta
     5881 gtacttcggc tactttaata ttccaataaa tatgatacaa tc
//
LOCUS       DAOSNN010000168         5909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13626_length_5909_cov_4.928425, whole genome shotgun sequence.
ACCESSION   DAOSNN010000168 DAOSNN010000000
VERSION     DAOSNN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13626_length_5909_cov_4.928425"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1046)
                     /gene="moaCB"
                     /locus_tag="PLQ70_08855"
     CDS             complement(135..1046)
                     /gene="moaCB"
                     /locus_tag="PLQ70_08855"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019386491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HQE34762.1"
                     /translation="MVNITFKSSTLRIAKAQAIVKVSKKETIDAIVNKTVPKGDVFEM
                     AKTAGLFAVKRTSDIIPDCHPIPVEFTAVRYNIKEMEITIEIEVHTIYKTGVEVEAMH
                     GASVVALTMYDMLKPIDKGIEIHSIKLLEKKGGKSDFTDKFRTDLKAAVIVCSDSISA
                     GTKFDKAGKAIIQKLETCKVGISDYSIISDDTLGIQEKVLDLYAQNIDLIILTGGTGL
                     SPRDNSPEAIKPLLDREIPGVMEAARNYGQDRTPFSMLSRGIAGMKGKTLIMTLPGST
                     KGAQESMDALFPSLLHIFRVVEGKQHE"
     gene            complement(1060..1506)
                     /locus_tag="PLQ70_08860"
     CDS             complement(1060..1506)
                     /locus_tag="PLQ70_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HQE34763.1"
                     /translation="MKEKKKHNVFVDGPISPEFIANSIAKHSTKTEIGAHDIFLGQVR
                     KDEIEGKDVVAINYEAYQEMADELFHEIREAAFVKYNLVCMHIYHSLGVVKAGEICLF
                     VFVSSKHRKVAFEACQDIVEQIKAMTPIWGKEIFEDDSYVWKENQI"
     gene            complement(1519..2631)
                     /locus_tag="PLQ70_08865"
     CDS             complement(1519..2631)
                     /locus_tag="PLQ70_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HQE34764.1"
                     /translation="MNSKPNNRYQRQIQLKEIGQEGQDKITQAKVLIIGAGGLGCPAL
                     QYLAAAGVGTIGIVDFDVVELSNLQRQILYNLDDIGKPKATTAAGKIKALNLEIQIET
                     YNLQITNKNALEILENYDLIIDGTDNFATRYLINDACVILDKPLIYGAVLRFEGQIGV
                     FNYSNFETNIKTNYRDLFPKPPNLDSAISCNDVGVLGVIPGIIGTMQATEALKIICGI
                     GKPLANKIVSYNVLENSFYDFEIAANENSNSDFPKNKAEFLAFNYECFCNANLDVETI
                     SIEEFDTIRKQETITIIDVREIGELPVVDEFAFLHIPLSELEKTLSTLSTENIIVVYC
                     KSGQRSAKAVQLLKEKFPNSQSFSLKGGIEAWKNIN"
     gene            complement(2673..2906)
                     /locus_tag="PLQ70_08870"
     CDS             complement(2673..2906)
                     /locus_tag="PLQ70_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQE34765.1"
                     /translation="MEINLLAFGQIVEITGKSAWKMAGIKDTNALIQDLEKQFPHLVK
                     TNYSIAVNKKVIQENTAINENDTIALLPPFSGG"
     gene            complement(2908..3477)
                     /locus_tag="PLQ70_08875"
     CDS             complement(2908..3477)
                     /locus_tag="PLQ70_08875"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HQE34766.1"
                     /translation="MKNGYILAGGKSSRMGADKGLLLFEGKAMIEYVINQMLPVFDNL
                     VIVSNNPEYEKFGLEVIPDLIKNIGPAGGIYTALHHSNAKLNFIVSCDMPFVTQEAIE
                     FVVNNTNESQIVLIENKGRIEPLFGVYSKECEENWLQLIEQKIIKLQDMVLQFKLKTI
                     PIENKEIFAASFFKNINTQEDFNNALNLL"
     gene            complement(3499..3831)
                     /locus_tag="PLQ70_08880"
     CDS             complement(3499..3831)
                     /locus_tag="PLQ70_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQE34767.1"
                     /translation="MKNLIIKGHLSIENEKGHLMGSGKKELLEKIKETGSIKAAASEM
                     KLSYRHAWEMIKKMNAISDAPLVIKNTGGSSGGGSVVTKEGEELIACYIKLNVAFEEF
                     KEQICKTI"
     gene            complement(3854..5029)
                     /locus_tag="PLQ70_08885"
     CDS             complement(3854..5029)
                     /locus_tag="PLQ70_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQE34768.1"
                     /translation="MINVKEAKQLIDENCCLMKSETLTLLEANGSILAEPIYAAIDTP
                     PFHQSAMDGYAFSYENWDKKSNLTLVGEIQTGNFSNSEVLANQVVRIFTGAPIPPGTD
                     TVVMQEKISREGNTIQILDDLLVKGTNVRPQGSQTQKGELALQENQLLTPVAISFLAG
                     IGINTVKVFSKPTISIIVTGKELASATDEITEGKIFESNSIGLIAALQQLGINPVSVE
                     VVDDVEAEIQKAISNQLSSDILILTGGVSVGDYDLVPASLEKCGVEKIFHKIKQKPGK
                     PFYFGRHNQTLVFALPGNPAAVMSCFYEYVVQAISSFTQKEYFKKMAFPLAEDFNKKA
                     GLTFFLKGKMGEKEVTVLNNQESYKLNSFAVADCLVEFDEEKEIFKKGDLVQVRMIL"
     gene            complement(5085..>5909)
                     /locus_tag="PLQ70_08890"
     CDS             complement(5085..>5909)
                     /locus_tag="PLQ70_08890"
                     /inference="COORDINATES: protein motif:HMM:NF018201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQE34769.1"
                     /translation="REFVKLGVKKIRLTGGEPLVRKDFREIVLALSKYPIELTLTTNG
                     LRTNEFIDVFKKAGIKSINVSLDTLDKDQFFTITKRNVFEKVWNNIQQLIDEGFHVKI
                     NTVVMRGINDTEILDFIEWTKNQPIHVRFIEFMPFDKNQWNTQKVFSYQEILDVAATK
                     YSFIRMKDGLNETAKKFKPFGHEGTFAVISTMSAPFCSNCNRMRLTADGKMKNCLFSK
                     GEADILSALRNSEDIVPIIKQCVASKAEALGGQFEGDYTKIDAAKIDNRSMINIGG"
BASE COUNT         1754 a         1157 c          897 g         2101 t
ORIGIN      
        1 tgaaagatgt agaaatgaaa aatcttataa cagaaaatca aaatttaaaa aataaatcaa
       61 ttggacttca actactattt ataaaaacaa aaaacgaatc tgtattatat ttttttaagt
      121 tttatttaag aactctattc gtgttgtttc ccctcaacaa ctctaaaaat atgtaaaagt
      181 gacggaaaca aagcgtccat cgattcttgc gccccttttg ttgaacccgg caaagtcata
      241 attagggttt tacctttcat tccagcaatt ccacgagaca acatcgaaaa aggagtgcga
      301 tcttgtccat aatttctagc agcttccata acaccaggga tttctctgtc taaaagtggt
      361 tttatggctt ctggcgaatt atctcttggc gaaagacctg ttccacctgt caatataata
      421 aggtctatgt tttgtgcata taaatccaat actttttctt gaattcccaa agtatcgtca
      481 gaaataatcg aataatcgct aatgccaacc ttacaagttt ctaatttttg gataatggct
      541 tttccagctt tatcgaattt agttcctgcc gaaatactat ccgaacacac aatcacagcc
      601 gcttttaaat cggtgcggaa tttatctgta aaatcagact tccctccttt tttctccaat
      661 aattttatag aatgtatttc gattcctttg tcgataggtt tgagcatatc atacatcgtc
      721 aacgccacca ccgaggcacc gtgcatcgcc tctacctcaa cacctgtttt gtaaatagta
      781 tgcacttcga tttcgatagt aatttccatc tcctttatat tgtatctaac cgctgtaaat
      841 tcgactggaa ttggatgaca atccggaatt atatcgctgg tgcgttttac tgcaaaaagt
      901 cctgcggttt ttgccatttc aaaaacatct ccttttggaa ccgttttatt gacaatagcg
      961 tcaatagttt cttttttact gacttttaca atggcttgcg cttttgcaat gcgaagagtg
     1021 ctcgatttga atgttatatt aaccatttga attttagttt tagatttgat tttctttcca
     1081 aacataagaa tcatcttcga aaatttcttt tccccagatg ggtgtcattg cttttatttg
     1141 ttctacaata tcctgacaag cttcgaaagc tactttgcgg tgtttggatg aaacaaatac
     1201 aaacaggcat atttcacccg cttttaccac tccaaggctg tgataaatgt gcatacaaac
     1261 caaattgtat ttgacaaaag cagcttctcg gatttcgtga aaaagttcgt cggccatttc
     1321 ttgataggct tcatagttga tagcaactac atcttttcct tcaatttcat cttttcgaac
     1381 ttgtcccaaa aaaatatcgt gtgcaccaat ttcggttttt gtactgtgtt tggcaatgct
     1441 attggcaatg aattcgggac ttattggtcc atcaacaaac acgttgtgct tttttttctc
     1501 tttcatttta aatgtttttt aatttatatt tttccaagct tcaattcctc cttttaaact
     1561 aaaggattga ctattaggga atttttcttt taataattgt actgctttgg cactccgttg
     1621 acccgatttg caatagacta caattatatt ttctgtcgaa agggtcgaaa gtgttttttc
     1681 caattcagac aatggaatgt gtaaaaatgc aaactcatca acaactggca attcccctat
     1741 ttctcgaaca tcaattattg tgattgtttc ttgctttcga atagtatcaa attcttcgat
     1801 tgaaatcgtt tcgacatcca aattggcatt gcaaaaacat tcgtaattga aagctaaaaa
     1861 ctcagcttta ttttttggaa aatcactgtt tgagttttca tttgcagcaa tttcaaaatc
     1921 ataaaatgaa ttttctagaa cattatagga aactatttta ttagctaatg gttttccaat
     1981 accacaaata attttcagag cttcggtggc ttgcatagtt ccaataattc ctggaattac
     2041 tcccaaaaca ccaacatcat tgcaagaaat cgcagaatct aaattgggtg gttttggaaa
     2101 taaatcacga taattagttt taatattggt ttcaaaattg ctgtaattaa aaacgccaat
     2161 ttgaccttca aaacgcaaaa ctgctccata aatcaaaggc ttgtcgagaa taacacaagc
     2221 atcatttatg agataacgtg tggcaaaatt atcggtacca tcaataatta aatcataatt
     2281 ttctagaatt tcaagcgcat ttttattggt gatttgtaaa ttgtatgtct caatttgaat
     2341 ctcaagattg agtgctttta tttttccagc ggcagttgtt gctttgggtt tgccaatgtc
     2401 atccaaatta taaagaattt gacgttgcaa atttgacaat tctacaacat caaaatcgac
     2461 aataccaatc gttcctacgc cagcagcagc aaggtattgc aaagctggac atcccaaacc
     2521 tcctgcgcca ataatcagca cttttgcttg tgttattttg tcctgacctt cttgcccaat
     2581 ttctttgagc tgaatttgtc tttggtatcg gttattaggt tttgaattca tatttaaatt
     2641 tattggtaaa gtttcgatag tttaatctcc tttcaaccgc cagaaaatgg aggaagtaaa
     2701 gctatcgtat cattttcatt gattgctgta ttctcttgaa tcactttttt gtttacagca
     2761 atcgaataat ttgttttgac caaatgggga aattgctttt ctaaatcctg tatcaaagca
     2821 tttgtgtctt ttattcctgc cattttccac gcagatttac ctgtaatttc aactatttga
     2881 ccaaatgcta gtaaattaat ttccatttta taatagattt aatgcgttat taaaatcttc
     2941 ttgggtgttg atgtttttga aaaaagaggc tgcaaagatt tctttatttt caattggaat
     3001 ggtcttcaat ttaaattgca aaaccatatc ttgtaattta attatttttt gttcgataag
     3061 ttgcaaccaa ttctcttcac attctttaga atatactcca aacaaaggct ctattcttcc
     3121 tttattttct attaaaacaa tttgtgattc attggtgtta ttgacaacaa attcaattgc
     3181 ttcttgcgtt acaaaaggca tatcacaact cacaataaaa ttcagctttg cattcgaatg
     3241 atgtaatgca gtgtaaattc cgccagctgg tccaatattt ttgatcaaat caggaattac
     3301 ttccagacca aatttttcgt actcgggatt attagaaaca ataaccaaat tgtcaaaaac
     3361 aggcagcatt tgattgatta catactcaat cattgctttt ccttcaaaca gaagcaaacc
     3421 tttgtcagca cccattctag aactttttcc acctgccaaa atatatccgt ttttcatttt
     3481 actgttcata acaaaaaact aaatcgtttt gcaaatttgc tccttaaatt cttcgaaagc
     3541 aacattcaat ttgatgtagc aagctatcaa ttcttcgccc tcttttgtaa caacagaacc
     3601 gccaccactg gatcctcccg tgttttttat aacaagaggt gcatctgaaa tggcgttcat
     3661 tttttttatc atttcccaag catgacgata cgaaagtttc atttcgcttg cagccgcttt
     3721 gatagaacct gtttccttga ttttttctag caattctttt ttgcccgacc ccatcaaatg
     3781 ccctttttcg ttttctattg aaagatgtcc ttttattatt aaatttttca tgtttttaat
     3841 gatataaata gcgttacaaa atcattcgaa cttgaaccaa atcgcctttt ttgaaaatct
     3901 ctttttcttc atcaaattct accaaacaat ccgcaacagc aaaagaattc aacttataac
     3961 tctcttgatt attcaaaacc gtcacctctt tttcgcccat ttttcctttc agaaaaaagg
     4021 tcaaaccagc ctttttatta aaatcttcgg ccaatggaaa tgccattttt ttgaaatatt
     4081 ctttctgtgt aaaactgcta atagcctgaa ccacatattc ataaaaacaa ctcattaccg
     4141 ccgcaggatt tccaggtaaa gcaaatacca aggtttgatt gtgacgccca aaataaaagg
     4201 gtttacccgg tttttgttta attttatgga atattttctc cactccacat ttttctaaag
     4261 aagcgggaac caaatcataa tcacctactg aaactccgcc tgttagaatc aaaatatcgg
     4321 aacttaattg attggaaata gctttttgaa tttctgcttc tacatcatcg acaacttcta
     4381 ccgaaactgg atttatgcct aattgttgta atgctgctat taacccaatg gagttggatt
     4441 caaaaatttt tccttcggtg atttcatcgg ttgccgaagc cagttctttc cctgttacaa
     4501 taatactgat agttggcttc gaaaaaacct tcaccgtatt gataccaatt ccagctaaaa
     4561 aagaaatggc aacgggtgtc aataattgat tttcttgcaa tgccaattcc cctttttgag
     4621 tttgtgaacc ttgaggacga acgtttgttc cttttactaa taaatcatcc agaatttgaa
     4681 tcgtatttcc ctcacgactg attttttctt gcattacaac cgtgtctgtc cccggtggaa
     4741 ttggcgctcc agtaaatatc cttacgacct ggtttgcaag tacttctgaa ttagaaaaat
     4801 tgccagtttg aatttctcca acaagagtta aattgctttt tttatcccaa ttttcataag
     4861 aaaaggcata accatccata gccgattgat ggaaaggtgg tgtgtctatt gcagcataaa
     4921 taggctcagc caaaatgctt ccgttggctt cgagcaaggt taaagtttct gatttcatca
     4981 aacagcaatt ttcatctatt agctgtttgg cttcttttac atttatcata attctaatta
     5041 tttcgatatg cctaaaaagg cataacggta ttcaaaaaaa agggttatcc tcctatgttt
     5101 atcatacttc tgttgtcaat tttggcagcg tcaattttgg tgtaatcgcc ctcaaactgt
     5161 ccgcccaatg cttccgcttt gcttgctaca cattgtttta ttatgggtac aatatcttcg
     5221 gaatttcgta atgccgacaa aatatcggct tcgccttttg aaaataaaca atttttcatt
     5281 ttaccgtctg ccgtcaatcg cattcgatta caattgctgc aaaacggtgc gctcattgta
     5341 ctgattacgg caaaagtgcc ttcgtgacca aagggtttga attttttggc ggtttcattc
     5401 aagccgtctt tcattcggat gaaactgtat tttgttgcag ccacatccaa aatttcctga
     5461 taactaaata ctttttgggt gttccattgg tttttatcaa aaggcataaa ctcaataaaa
     5521 cgaacgtgta taggttgatt ttttgtccat tcaataaaat caagaatttc agtatcattt
     5581 ataccgcgca ttacaacggt atttatcttt acgtgaaagc cctcgtcaat aagttgctga
     5641 atgttgttcc atactttttc gaaaacattg cgtttggtaa tggtaaaaaa ctggtcttta
     5701 tcgagtgtat ccaaacttac attaatcgat tttattccag cttttttaaa aacgtcgata
     5761 aactcattgg ttcgcaaacc gtttgtggtc aaagtaagtt caattggata ttttgaaagt
     5821 gccaaaacaa tttctctaaa atcttttcta accaaaggtt ctcctccggt aaggcgtatt
     5881 tttttgactc ccaatttcac aaattcccg
//
LOCUS       DAOSNN010000169         5835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13841_length_5835_cov_5.966263, whole genome shotgun sequence.
ACCESSION   DAOSNN010000169 DAOSNN010000000
VERSION     DAOSNN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13841_length_5835_cov_5.966263"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLQ70_08895"
     CDS             <1..806
                     /locus_tag="PLQ70_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HQE34770.1"
                     /translation="PNFNIVGEISMRDQAQLSYWQKDSPIGKIQNFNSHLPSVMDFNL
                     SDELLQVFNVDEGKWGLGMDKIYDNFTNDFLFPNPNNLLIFAENHDTHRVNHIYQNDI
                     RKYKMAMALIATARGIPQTYYGSEVGMAGNKDLGDAAIRQDFPGGWEGDTNNAFTKEG
                     RSQVQNDYFDFTSKLFNWRKNKSVVHFGKMTHYIPENNTYVYFRHNATEIVMVAFNNS
                     NETKTLKTERFAENIGNFKTGKDIITDQLIDITQEVRLEPKSVLILELK"
     gene            872..>2336
                     /locus_tag="PLQ70_08900"
     CDS             872..>2336
                     /locus_tag="PLQ70_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018941956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQE34771.1"
                     /translation="MKKLLFLLLISSLSFAQNANRKFESFKEVNNTLEINTSEGQYII
                     KAYSEKIVETSFIPKGETFNPNSQAVVLAPKTRISKIIETPSFIHLYTKGILVSVQKS
                     PFQIFYGKGNQMFLSEKNGYSKKENAEVIDFNLDSSEMIYGGGARVLGMNRRGNRLQL
                     YNRASYGYETRADLMNFCIPMVLSSKMYAVHFDNSAIGYLDLDSKKENTLAYETISGR
                     KTYQVIVGDSWTDLISNYTDLTGKQPLPARWTLGNFSSRFGYHSQEEVEKTIKKFEDD
                     QIPVDAVILDLYWFGKEMKGTMGNLDWEKETFPNPEKMMADLNAKGIKTVLITEPFVL
                     TTSKKWQEAVDKKVVATDKNGKPFTYDFYFGNTGIVDIFKPDGKDWFWNVYKRLIHQG
                     VGGLWGDLGEPEVFPSEALTAGGTADEIHNVYGHNWAKLIAEGYKKDFSNQRPFILMR
                     AGYSGSQLFGMMPWSGDVSRSWGGLQSQTEISLQMGMQ"
     assembly_gap    2337..2436
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2437..3390
                     /locus_tag="PLQ70_08905"
     CDS             <2437..3390
                     /locus_tag="PLQ70_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQE34772.1"
                     /translation="SWGGLQSQTEISLQMGMQGMAYMHSDLGGFAGDYFDNELYLRWL
                     QYGVFQPIFRPHAQEEVASEVARKDIITKAKAKKLVELRYQLLPYNYTLAFENSQKGT
                     PLMRPLFFEEPNNTKLLTVCETYLWGNDFLVTPITKAGVTSTSVYFPKNNNWFDFYSD
                     EEQLAGTTSTITVVEDHIPVFVRGGAFIPMIKTIQNTSKYSLDNFDLHYYFDAKTTQS
                     SGKLYNDDGKTANAFEKGAFEVLNFNGNTNGKTVVVKLSSEVGKNFPSSDKNVSFIVH
                     NIKAKSVTGNGKTIAFKTVKNNIEIPVSWKKGTAMEIKIQL"
     gene            3444..5096
                     /locus_tag="PLQ70_08910"
     CDS             3444..5096
                     /locus_tag="PLQ70_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQE34773.1"
                     /translation="MKKNIIFSIAFLSLLFISCSSTKTSTASSKTPFVWENANVYFLL
                     IDRFNNGDKTNDHTFNRNKPTGKLRGFEGGDIRGVIQKLDEGYFEKLGINAIWMTPVV
                     EQIHDGVDEGTGYSYGFHGYWTRDWSALDPSFGTKKDLAELVQKAHAKGIRILLDAVI
                     NHTGPVTTEDAAYPNDWVRTSPKCTYTSYDTYINCTLVENLPDVKTESNEVVALPPFL
                     VEKWKKEGRYEQEVAELDAFFTKTGYPRAPKYYIMKWLSDYITDYGIDGYRVDTAKHT
                     YEDVWADFKKVCDQSFADFKKNNPEKVLDNNAFFTVGEVYGYNIGAKKLFDFGDRKVD
                     YFANGFTGLINFDFRNEAKMNYEKLYSKYNSILQNDLKGNTVMNYVSSHDDSSPYDKK
                     REKTFESGTKLLLAPGISQVYYGDESARPLDIPGTIGDATLRSMMNWEEIKTNPETQK
                     VLLHWQKLGNFRKNHPAIGAGIHQEISASPYVFSRTFTQDNFTDNVVIGLDLPKGPKE
                     ISVGTIFENGTKLKDAYSGKKVVVSNGKVSIDSEFDIVLLEK"
     gene            5203..>5835
                     /locus_tag="PLQ70_08915"
     CDS             5203..>5835
                     /locus_tag="PLQ70_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQE34774.1"
                     /translation="MNQLLKIGHRGVKGYEPENTLISFQKAIDLKVDGIELDVHLSSD
                     GEIIVIHDETIDRTTNGKGLVKNFSSLQLKQFGIPTLSEVLELINKQCLVNIELKGIG
                     TSKPVAEFIALFISQKNWNHTDFLVSSFDWKMLEEVQLLNPKIRIGVLTEESVDLALA
                     FAKKIKAYSIHPDYRLLTKENVALMQENEFEVYPWTVNSKEDIQKIKSFNV"
BASE COUNT         1977 a         1042 c         1100 g         1616 t
ORIGIN      
        1 atccaaattt taatatcgtt ggcgaaattt cgatgcgcga tcaagctcaa ctttcctatt
       61 ggcaaaaaga cagtccgata ggtaaaatcc agaattttaa ttcccattta cccagcgtaa
      121 tggattttaa tttatcagat gaattattac aagttttcaa tgtagatgaa ggaaagtggg
      181 gacttggaat ggacaaaatc tatgataatt tcaccaatga ttttctgttt ccaaacccta
      241 acaatttgct gatttttgcc gaaaatcacg acacacatcg tgtgaatcac atttaccaaa
      301 acgacattcg aaaatacaaa atggcaatgg ccttgattgc tacagcacga ggaattccgc
      361 aaacgtatta cggttccgag gtgggaatgg caggaaataa agacttaggt gatgccgcta
      421 ttcgccaaga tttccctggc ggttgggaag gcgataccaa taatgctttt accaaagaag
      481 gccgaagcca agttcaaaat gactatttcg attttacttc caaactcttc aattggagaa
      541 aaaacaaatc ggtcgttcat ttcggaaaaa tgacgcacta cattccagaa aacaatactt
      601 atgtctattt cagacacaat gcaactgaaa tcgtgatggt tgcattcaac aacagcaacg
      661 aaaccaaaac actaaaaacg gaacgctttg ccgaaaatat aggaaacttc aaaacgggga
      721 aagacatcat cactgaccaa ctaattgaca ttacacaaga agttagattg gaacccaaat
      781 cagttttgat attggaattg aaataattta aaccatatta gaaaaactta aatgacctta
      841 tatgacttat agggttcaaa aaacacaaac aatgaaaaaa ttacttttct tattattgat
      901 aagttcactt tcctttgccc aaaacgcaaa tagaaaattc gaaagcttca aagaagtcaa
      961 caatacatta gaaataaaca cttcggaagg tcaatatatc atcaaagcct attctgaaaa
     1021 aattgtagaa acctctttta ttccaaaggg agaaaccttt aatccaaact ctcaagccgt
     1081 cgttttagct ccaaaaacta gaatttcaaa aataattgaa acgccaagtt ttattcatct
     1141 ttacaccaaa ggaatattag tttcagttca aaaatcacct tttcaaattt tttatggaaa
     1201 aggaaatcaa atgtttttgt cggaaaaaaa cggctactcc aaaaaagaaa acgccgaagt
     1261 catcgatttc aaccttgaca gttccgaaat gatttacggt ggtggcgcaa gagttttggg
     1321 aatgaatcgt cgcgggaatc gtttgcaatt gtacaatcgc gccagttatg gttacgaaac
     1381 ccgagctgat ttaatgaatt tctgcattcc aatggtgtta tcgtcgaaaa tgtatgcggt
     1441 tcattttgac aattccgcta ttggctactt ggatttagac agcaagaaag agaatacttt
     1501 ggcgtatgaa accatttccg gccgaaaaac ctaccaagta atcgtgggcg attcctggac
     1561 ggatttgatt tcgaattata ccgatttaac tggaaaacaa cctttgcccg ctcgatggac
     1621 tttggggaat ttttcctctc gttttgggta tcactcccaa gaagaagtcg aaaaaaccat
     1681 caagaaattc gaagacgacc aaattcctgt cgatgccgtg attctcgatt tgtattggtt
     1741 tggcaaagaa atgaaaggca caatgggcaa cttagattgg gaaaaagaaa ccttcccaaa
     1801 ccccgaaaaa atgatggccg atttgaatgc caaaggaatc aaaacagttc tcattaccga
     1861 accttttgta ctcacaactt ccaagaaatg gcaagaagcc gtggacaaaa aagtggtggc
     1921 aaccgataaa aacggaaagc cttttaccta tgatttctat tttggaaaca ctggaatcgt
     1981 agatattttc aaacccgatg gaaaagactg gttttggaat gtgtacaaac gattaatcca
     2041 tcaaggtgtt ggcggacttt ggggcgattt gggcgaaccc gaagttttcc cttccgaagc
     2101 cttgactgct ggcggaacag ccgacgaaat ccacaatgtt tacggacaca attgggcaaa
     2161 attgattgcc gagggttaca aaaaagactt ttctaatcaa cgtccattta tcttaatgcg
     2221 cgctggatat tcgggttctc aactttttgg gatgatgcct tggtctggcg acgtgagccg
     2281 ctcttggggt ggattgcaat cgcaaaccga aatttccctt caaatgggaa tgcaggnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntctt ggggtggatt gcaatcgcaa
     2461 accgaaattt cccttcaaat gggaatgcag ggaatggctt atatgcactc ggatttgggc
     2521 ggttttgccg gcgattattt cgacaacgaa ttataccttc gttggttgca atacggcgtt
     2581 ttccaaccga tatttcgtcc gcacgcccaa gaagaagtgg catcagaagt ggctcgcaaa
     2641 gacatcatca ccaaagcaaa agccaagaaa ctagtagaat tgcgttacca acttttaccg
     2701 tataactaca ctttggcttt cgaaaacagc caaaaaggaa cacctttgat gcgaccttta
     2761 ttttttgaag aacccaacaa caccaaatta ttgaccgttt gcgaaaccta tctttggggg
     2821 aatgacttct tggtgactcc aataaccaaa gcgggtgtga cttccacttc ggtatatttt
     2881 ccaaaaaaca acaattggtt tgatttctat tccgatgaag aacaattggc tggaacgact
     2941 tcaaccatta ccgttgttga agaccatata cctgtatttg ttcgtggagg cgcttttatt
     3001 ccaatgataa aaaccatcca aaacacatcg aaatattctt tagacaactt cgatttgcat
     3061 tattattttg atgcgaaaac cactcaaagt tccggaaaat tatacaatga tgacggaaaa
     3121 acggcaaacg cttttgaaaa aggagcattt gaagttttga attttaatgg caataccaat
     3181 ggcaaaacag tagttgttaa attgagttct gaagttggga aaaactttcc atcatctgat
     3241 aagaatgttt catttatcgt tcacaatata aaagcaaaat cagtaaccgg aaacggaaaa
     3301 actattgctt tcaaaaccgt taaaaacaat atagaaattc cagtttcttg gaaaaaagga
     3361 acagcaatgg aaatcaaaat tcaattataa tttataaaac ctaaaaggtt ttataaacct
     3421 gttaggttca aaacaaaaca aatatgaaaa aaaatatcat cttttcaatc gcatttcttt
     3481 cgctgctttt catcagttgt tcttcaacaa aaacatcgac agcatcatca aaaactcctt
     3541 ttgtttggga aaatgctaat gtgtattttc ttttaatcga ccgattcaac aatggagata
     3601 aaacgaacga tcatactttc aatagaaata aaccaacggg aaaattgcgt ggttttgaag
     3661 gaggtgatat tcgaggtgta atccaaaaat tagacgaagg ttactttgaa aaactgggga
     3721 taaatgccat ttggatgacg cccgttgtcg aacaaattca cgatggagtt gacgaaggaa
     3781 ctggttatag ctatggattt cacggctatt ggaccagaga ttggtcagca ttagatccaa
     3841 gttttggaac taaaaaagat ttggccgaat tggttcaaaa agcgcacgca aaaggaattc
     3901 gtattttatt ggatgctgtc atcaatcaca ccggacctgt tactactgaa gatgccgctt
     3961 atccaaacga ttgggtccga acctcgccaa aatgtactta cacatcctat gacacttata
     4021 tcaattgcac cttggtcgaa aatcttccag atgtaaaaac cgaaagcaat gaggttgtag
     4081 ctttgccgcc atttttagtt gaaaaatgga aaaaagaagg tcgttatgaa caagaagtag
     4141 ccgaattgga tgcgtttttc accaaaacag gttatcctcg tgcgccaaaa tattacatta
     4201 tgaaatggct gtcagattac atcaccgatt atggaattga tggctatcga gtggatacgg
     4261 cgaagcacac ttatgaagat gtttgggctg atttcaagaa agtttgcgat caatcctttg
     4321 cagatttcaa aaaaaacaat cctgaaaaag tattggacaa caatgcgttt tttaccgttg
     4381 gcgaagtgta tggctacaac attggagcaa aaaaactatt tgattttgga gacagaaaag
     4441 tggattattt cgcaaatgga tttactggtt taatcaattt tgattttaga aacgaagcca
     4501 aaatgaatta cgaaaaatta tattctaaat acaattcaat acttcaaaat gacttgaaag
     4561 gcaatacggt gatgaactat gtttcttcgc acgatgacag ttctccttat gacaagaaac
     4621 gtgaaaaaac cttcgaaagt ggcacaaaat tattgcttgc gccgggaatt tcacaggttt
     4681 attatggcga cgaaagcgct cgtccgctag acattccggg aactatagga gatgcaactt
     4741 tgcgttcgat gatgaattgg gaggaaataa aaaccaatcc cgaaacccaa aaagtgcttt
     4801 tacattggca aaagctaggt aatttcagaa aaaatcatcc cgccattgga gcaggaattc
     4861 atcaagaaat ttcggcaagc ccttatgttt ttagccgaac ttttacccaa gataatttca
     4921 cggataatgt ggttattgga ctggatttac caaaaggacc gaaagaaatt tcagtaggaa
     4981 ccattttcga aaacggaacg aaattgaaag atgcttattc tggaaaaaaa gtcgtggttt
     5041 cgaatggaaa agttagtatt gatagcgaat ttgatatcgt tttattggaa aaataattca
     5101 gagcaagaat tcaaacaatt attcgcaaag ttcccaaagt aaatgaatcc tttgggaact
     5161 ttgctttttt cttaagtacc tttgcggtaa atttagctgc aaatgaatca acttctcaaa
     5221 attggacatc gtggtgtaaa agggtatgaa cctgaaaata ctttaatctc ttttcaaaaa
     5281 gcgattgatt tgaaagtgga cggcatcgaa ctggacgtac atttgagttc ggatggtgaa
     5341 ataatagtca ttcacgacga aactattgac agaactacca atggaaaagg tcttgtaaaa
     5401 aatttttctt ctttgcaatt aaaacaattt ggaattccaa ctttatccga agttttagaa
     5461 ttgattaaca agcaatgtct tgtgaatatt gagctaaaag gaattggaac ttcaaaacct
     5521 gtagccgaat ttattgctct tttcatttca caaaaaaact ggaatcacac tgattttcta
     5581 gtttccagtt tcgattggaa aatgttggaa gaagtacaat tattgaatcc aaaaatccga
     5641 attggtgttt tgactgaaga atctgttgac ttagctttgg ctttcgccaa aaaaataaaa
     5701 gcctattcca ttcatcctga ttatagattg ctaacaaaag aaaatgtagc tttgatgcaa
     5761 gaaaacgaat tcgaagttta tccttggact gtgaattcaa aagaagacat tcaaaaaata
     5821 aaatcattca acgta
//
LOCUS       DAOSNN010000170         5818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13901_length_5818_cov_4.577824, whole genome shotgun sequence.
ACCESSION   DAOSNN010000170 DAOSNN010000000
VERSION     DAOSNN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13901_length_5818_cov_4.577824"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLQ70_08920"
     CDS             complement(<1..508)
                     /locus_tag="PLQ70_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQE34775.1"
                     /translation="MKYKGIIFDLDGTLVNSVEDIANAMNSVLKSLNFPTHSYEDYQY
                     FVGSGLRNLVSKALPSSHNDETQIDHCFQLMIEVYRENCTHQTKYYEGIIELLDYLKA
                     HNIKLSVFSNKADELTKKIVAVLFPNYFIEVVGLSIESLKKPNPFEALEICKKLELKP
                     EEMIFVGDS"
     gene            1415..2497
                     /locus_tag="PLQ70_08925"
     CDS             1415..2497
                     /locus_tag="PLQ70_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQE34776.1"
                     /translation="MKRIIAFTGILAVLSLASCANKKEEKEEVTNFAVTNPVQIDTSF
                     TKQYVAQIKSFRNIEIRAQEKGFLQNIYVDEGQFVKKGQLLFQIMPKVYEAELLGAQA
                     EEKAAEIELQNAKTLADKNIVSKNEQAVAQAKLQQARAEVSLAKLHLSFTQIRAPFDG
                     TIDRIPKKLGSLIDEGELLTSLSDNSQMYAYFNLSEPEYLDYETNIKNRAASNVGLLL
                     ANGNTFKDKGNVEVVESEFDNETGNIAFRARFPNLAKLLKHGETGKVLMEVPLHNVIV
                     IPQKATYEIQDKKYVFVVNKNDVVSSKEITIKGEIPDLYMIDSGITATDKILLEGVQK
                     VKDNDKIHYKYIVPQEVITHLRLKAE"
     gene            2604..5774
                     /locus_tag="PLQ70_08930"
     CDS             2604..5774
                     /locus_tag="PLQ70_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQE34777.1"
                     /translation="MFNKFIQRPVMSIVISLIIVFLGVLAVLNLPITQFPSISPAIVN
                     VTADYPGSNGELMIKSVVIPLERALNGVPGMKYMTSDAGNDGEASIQIAFNLGTDPNQ
                     AAINVQNRVASVVNKLPPIVVREGVKITREVPSMLMYVNLYSTDKNTDQKFLYNYADI
                     NVVSELKRVNGVGSGDILGTREYAMRIWLKPDRMLAYKISADEIMEALSSQSIEASPG
                     KTGESSGKRSQAFEYVLKYSGRFTTKEQYANIILKSNPNGEILRLKDVANIEFGSSMY
                     DIYSNLNGKPSAAIVLKQSFGSNANQVIADVKAKLAAIEKKFPKGMKYEISYDVSNFL
                     DASIEKVIHTLIEAFILVGLVVFLFLGDWRSTVISALAIPVSLIGTFVFMQYFDISLN
                     LITLFAMVLAIGIVVDNAIVVIEAVHAKMEEENLSALKATKKAMHEIAGAIIAITFLM
                     VAVFVPVTFMSGPVGIFYRQFSITMATAIVLSGIIALTLTPALCAMMLKNNHGVSKKR
                     TPINRFIESFNNKFNLAQGKYQNVLSKIIDRRIITLISLLVFCAGTWLISTTVPSGFI
                     PNEDQGMFYAIIQTPPGSSLERTNDVALKLQKIAENVEGVKSVSSLAGYEILTEGTGS
                     NAGTCLINLKNWSDRKQSVQEIMAELEAKSKDIPGANIEFFQPPAVPGYGAAGGFELR
                     LLDKTGSGDYKKMEAVNSDFVKELNKRKELSNVFSFYSASFPQYMLKVDNDLAQQKGV
                     SIENAMNTLSTLVGSNYEISFIKYGFNYKVIVQAAPEYRALPEDILKLYVKNDRDEMV
                     PFSAFMKLEKVYGLSEITRHNMYTSTEISGAAAEGFSSGTAIKVIQEVAAAKLPRGYD
                     IDWAGISADEVAQGNQAIWVFLICLGFVYLVLAAQYESFILPFSVILSLPAGIFGAFL
                     LLSLTGLENNIYAQVAMVMLIGLLGKNAVLIVEFAIQRHAAGKTVFEAAIEGAKTRFR
                     PILMTSFAFIAGLLPLVFATGPGKIGNRTIGTAAVGGMLIGTICGVFVIPGLYYIFGK
                     IAERFTYVKNQKENPLTEEFDDNYAQ"
BASE COUNT         1927 a          877 c         1091 g         1923 t
ORIGIN      
        1 cagaatcgcc aacaaaaatc atttcttcgg gctttaattc taatttttta caaatttcta
       61 aagcttcaaa cgggttgggt tttttaagtg attcgatact caaacctaca acttcaataa
      121 aatagtttgg aaataaaacg gcaacaattt tcttggtgag ttcatcggct ttattagaga
      181 aaacactcag ttttatgttg tgagccttca gataatctaa taattcgata atgccttcat
      241 aatattttgt ttggtgggta caattctcac gatacacttc aatcatcaat tggaaacaat
      301 gatctatttg tgtttcgtcg ttatgagaag aggggagtgc tttactaact agatttcgga
      361 gtccgcttcc aacaaaatat tgataatctt cataactatg agttgggaaa ttgagacttt
      421 tcagcacgct attcatagca tttgcaatat cttcaaccga attgactaat gttccgtcta
      481 aatcaaaaat aattccttta tacttcattt tttatttttt gagttggggt ttatatcttg
      541 gatatgtttt tatagatttt gattcaattc ttaagaattg aaccaatttt agtgcagtaa
      601 aattagtcaa atattcagat atagcccaat tattgatttc agtatgttct cctatagtat
      661 gagtatttgc aattgaaaag gcatcattct tctactaatt ttggttaaat aaaattaatt
      721 ttttgaaaca acaaataaat tcagattgga gttatttgca aacataaatt caaaaaaaaa
      781 gttaaaattt atacattaat atctcatagt tgctaacttc gccacgatta tgagagcaat
      841 tgtttattac atatcactaa tttttcttct actatgtgga ggaaattatg tgtatgcaaa
      901 tactaatcaa agctctggaa ctttctcttg ctctaaaaat agttttgaaa aacagcattt
      961 aaaacataaa ggttccaaac atcatgatga atctattcag agttcagaca tggaattgga
     1021 cgaagagttt cacttagaag acgacttaga ttgtacaaat tcgaatgcta ttttagtaaa
     1081 caatcaaaat ctactgaata gttggtatat aactttttca aatcagttta tttttaagaa
     1141 actttttgaa agtactaaat ttttcgagcc atatacgggc caatccaatc ctatttacct
     1201 ccaaatagga attttaagaa tttgattcca tttcacatca attactaaag gtgtgatttt
     1261 aagttatcca atgtttgtat tttaatgttg gtttattaag tatttatcat tctccgacag
     1321 cttaggttcc accttagtaa ctgattgtca tttcttgact taaggaattt ttatattcca
     1381 aaatttcaat cactaaattt ctatttttta tattatgaaa agaataattg cgttcacagg
     1441 catacttgcc gtgttgtccc ttgctagttg tgcaaacaag aaagaagaaa aagaagaagt
     1501 tactaatttt gcggttacta accctgtgca aattgatact tcgtttacca aacaatatgt
     1561 agcacaaata aaatcattcc gaaatattga gattcgtgct caagaaaaag gttttctgca
     1621 aaacatttat gttgatgaag gtcagtttgt aaaaaaaggg caattattgt ttcaaattat
     1681 gccaaaagta tatgaagctg aattattggg agcacaagca gaagaaaaag ccgctgaaat
     1741 agaattgcaa aatgctaaaa cattggccga taaaaatatc gtttctaaaa atgaacaagc
     1801 tgttgctcag gctaagttgc aacaagctag agccgaagtt tccctagcca aactccattt
     1861 gtcttttacc caaataagag cgccatttga tgggactatt gatagaattc caaaaaaatt
     1921 aggaagccta atcgatgaag gtgagttgtt aaccagtctt tctgacaaca gtcaaatgta
     1981 tgcttatttt aatctatcag aaccagaata tttggattat gaaaccaata ttaaaaatag
     2041 agcagcgagc aatgtaggat tgcttttagc taatggaaac acatttaaag acaaaggaaa
     2101 tgtagaagtt gtcgaaagtg aatttgataa tgaaacagga aatattgcct ttagagcccg
     2161 atttcccaat ttagcaaaat tgttgaaaca cggcgaaact gggaaagttt tgatggaggt
     2221 tccgttgcat aatgtaattg tgattccaca aaaagcaacc tacgaaattc aagacaagaa
     2281 atatgttttt gtagtaaata aaaacgatgt tgtgtcttct aaagagatta caattaaagg
     2341 cgaaattcca gatttgtata tgattgatag cggaataaca gctactgaca aaatcttgct
     2401 agaaggcgtt cagaaggtta aagacaatga taagattcac tataaataca tagtgcctca
     2461 agaagtaata actcatttgc gattaaaagc agagtaaact taagtggtca gtaatcagta
     2521 atcagtattt agtttacagt attcagtaat tgtttactat ctaaaaaacg aattttggtt
     2581 taatcctaaa aaagtagaaa caaatgttta ataaatttat acaaagacct gttatgtcga
     2641 tagtaatatc gttaataatt gtctttttag gggttttggc ggtattgaat ttgcccataa
     2701 cccaatttcc ttccatttca ccagccattg tgaatgttac cgcagattat ccaggatcta
     2761 acggtgaatt aatgataaaa tcggttgtaa ttccattaga aagagctttg aatggagttc
     2821 caggaatgaa atatatgact tcggatgctg gaaatgatgg tgaagcaagt attcaaatag
     2881 cttttaattt gggtacagac cctaaccaag cagctattaa cgttcagaat cgtgtagctt
     2941 ctgtggtaaa taaacttcca ccaattgtgg taagagaagg agtgaaaatt acccgtgaag
     3001 ttccgagtat gttgatgtat gtcaatcttt atagtactga caaaaatacc gaccaaaagt
     3061 ttttgtacaa ttatgccgat atcaatgtgg tatccgaatt gaaaagggta aacggagtag
     3121 gttctggtga tattctagga actcgtgaat atgcgatgcg tatttggttg aaacccgatc
     3181 gaatgttggc ttacaaaatt tctgcagatg aaataatgga ggccttatcc agtcaaagta
     3241 tagaagcctc acccggaaaa actggggaaa gttcaggtaa acgttcacaa gcattcgaat
     3301 atgttttgaa atattcagga cgatttacta ctaaagaaca atatgccaat attatattga
     3361 aatccaatcc aaacggtgaa atcctgaggt tgaaagatgt ggcaaatatc gagtttggta
     3421 gttcgatgta tgatatttat tctaatttaa atggaaaacc ttcagcagca attgttttaa
     3481 aacaatcttt tggtagtaat gctaaccaag ttattgcaga tgtaaaagcc aaattagctg
     3541 ccattgagaa aaaatttcca aaaggaatga agtatgaaat ttcgtatgat gtttctaatt
     3601 tcttagacgc ttctatcgaa aaagtaattc acactttgat tgaagcattt attttggtgg
     3661 gattggttgt gtttcttttc ttgggcgatt ggcgttctac agttatttcg gctcttgcca
     3721 ttcctgtatc gttgattgga acttttgtgt tcatgcaata tttcgatatt tcgctgaact
     3781 tgattacgtt gtttgctatg gttttggcta ttggaatcgt ggtcgataat gccattgtgg
     3841 tcattgaagc cgttcacgcc aaaatggaag aagaaaacct ctcggcttta aaagcaacta
     3901 aaaaggcgat gcacgaaatt gctggagcta ttatagcaat tacatttttg atggttgccg
     3961 tatttgttcc agtgacgttt atgtctggtc cggttgggat tttctatcgt cagttttcta
     4021 ttacgatggc aacagctatt gttctttcgg gtattattgc attaaccttg actccagcac
     4081 tttgtgcgat gatgttgaaa aacaatcacg gggtttctaa aaagagaact ccaataaata
     4141 gatttatcga aagttttaac aacaaattta atcttgcaca aggcaaatat caaaatgtat
     4201 tgagtaaaat tatcgacaga cgaataatta ctttgatatc tcttttggtg ttttgtgctg
     4261 gaacctggct tattagtact actgttcctt ctggatttat tcctaatgaa gatcaaggaa
     4321 tgttttatgc gattattcaa actcctccag gttcatcatt agaaagaacg aatgatgttg
     4381 cgttaaaatt acaaaaaatt gctgaaaatg tagaaggcgt aaaatcagtt tcatcgctag
     4441 cgggatatga aattttgacc gaaggtactg gttctaatgc aggaacttgt ttgatcaatc
     4501 ttaaaaattg gagcgataga aagcaatctg ttcaagagat tatggcagaa ttagaagcta
     4561 aatctaaaga cattccggga gctaatatcg aattttttca accaccagcc gtacctggat
     4621 atggagctgc gggaggattt gaacttcgtt tattggataa aactggttca ggtgattaca
     4681 aaaaaatgga agctgtgaat agtgattttg taaaagaact caacaaacgc aaagaattat
     4741 ccaatgtatt tagtttctat agtgccagtt ttccacaata tatgttgaaa gttgataatg
     4801 atttagccca acaaaaagga gtttctatag aaaatgcaat gaatacttta tctactttgg
     4861 tgggaagtaa ttacgaaatc agttttatta aatatgggtt taattacaaa gtaatcgttc
     4921 aagctgctcc agaatatcgt gccttgccag aagatatttt aaaactttat gtcaaaaatg
     4981 atcgagatga aatggtacct ttctctgctt ttatgaaatt agaaaaagtg tatggtctgt
     5041 ccgaaatcac gagacataat atgtacactt ctactgaaat tagtggtgct gctgcagaag
     5101 gttttagttc tggaacagct attaaagtaa ttcaagaagt agctgctgct aaactaccaa
     5161 gaggatatga tattgattgg gcaggaattt ctgctgatga agtagctcaa ggaaaccaag
     5221 ctatttgggt ttttcttatt tgtttaggtt ttgtttattt ggttttggca gctcaatacg
     5281 agagttttat tttgcctttt tcggtaattc tatccttacc agccggaata tttggagcct
     5341 tccttttgtt gagtttaact ggtttagaaa acaatatcta tgctcaggtg gcaatggtaa
     5401 tgcttatcgg tttgctgggt aaaaatgccg ttctaattgt ggaatttgcc attcaacgac
     5461 acgctgcagg aaaaacagta ttcgaggcgg ctatcgaagg tgcaaaaacc agatttcgcc
     5521 ctattttgat gacctcattt gcttttattg caggtttact tccgttggtg tttgctacag
     5581 gacctggaaa aattgggaac cgaacaattg gtaccgctgc agtaggagga atgttgatag
     5641 gaaccatttg tggcgtattt gtaatccctg gtttgtatta tattttcggt aaaatagccg
     5701 aaagatttac ttatgttaaa aatcaaaaag aaaatccatt aaccgaagaa tttgacgata
     5761 attatgctca ataaaaagtt atttaaatat agtgttccgc taagcatttg cttagcgg
//
LOCUS       DAOSNN010000171         5808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13934_length_5808_cov_6.171215, whole genome shotgun sequence.
ACCESSION   DAOSNN010000171 DAOSNN010000000
VERSION     DAOSNN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13934_length_5808_cov_6.171215"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLQ70_08935"
     CDS             complement(<1..854)
                     /locus_tag="PLQ70_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQE34778.1"
                     /translation="MSSQDIITIEDDFTLLRFQNDSDEVYCTQREVKSGLIQFHFGLK
                     GKAKFLFNQGSYALDLKEEKSLLLYNPQKELPLNLEIAPNSWVISVIISIQKFHNLFS
                     SEANYITFLSEDNRDKKYYKEGDISPSMAIVLTQLFHYNLHPSIKNLYYKGKGYELLS
                     LYFNRTEDPNAEQCPFLIDEENVLKIKKAKELVLANIAEPPSLQELADEIGLTLKKLK
                     MGFKQIYGDTVYGFLFDYKMDSARKLLDSGSYNVNEVGLKIGYSTGSHFIAAFKKKFG
                     TTPKKYLMA"
     gene            1078..>2330
                     /gene="hemA"
                     /locus_tag="PLQ70_08940"
     CDS             1078..>2330
                     /gene="hemA"
                     /locus_tag="PLQ70_08940"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HQE34779.1"
                     /translation="MENLNIPKHKTFYSIGLSYKKADAEVRGKFSLDAKTKTKVLLQA
                     KEEGIESLIVTSTCNRTEIYGYADHPFQLIKLICENSNGTVEEFQKAGFVYKNQEAID
                     HMFRVGTGLDSQILGDFEIIGQIKNSFEEARSLGLINAFLDRLINTVIQTSKRIKNET
                     EISSGATSVSFAAVQYIIRNIEDIGNKNILLFGTGEIGRNTCENLVKHTKNEQITLIN
                     RTKSSAEILAGKLNLVVKDYSDLHLELQKADVLVVATGAQNPTIDKTLLNLKKDLLVL
                     DLSIPKNVNADVQQIPGVTLIHMDYLSQMTDDTLERRKQHIPAAEAIIEDMKLELNTW
                     MNGRKYAPTIHALKAKLNDIVAGELTFQRKKLSNFDEEQAELITSRIIQKITNQFASH
                     LKDENTSVDESIEFIEKVFQIGLQTP"
     assembly_gap    2331..2430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2431..2906
                     /locus_tag="PLQ70_08945"
     CDS             <2431..2906
                     /locus_tag="PLQ70_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HQE34780.1"
                     /translation="MQKLTESDWSGAVFAAAGLERINLKPTDFINLDWMIPAPAQGAM
                     VVVAMGNDEFVLDAVSQLNDVETEICTYIERQFLKTLEGGCTAPIGALAKYCELEDTF
                     TFKGALLSIDGKQKLEIEKIVPVEEWKKLGFNCAKEILENGGAELMKTIKESLKK"
     gene            2906..3571
                     /locus_tag="PLQ70_08950"
     CDS             2906..3571
                     /locus_tag="PLQ70_08950"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQE34781.1"
                     /translation="MSNPISILSTKILLSHQKQALLNAGFSVTEANFIKTENSDFDLK
                     GINDNLIFTSQNAAQSVLLHPKCGELKSKNVFCVGMKTKALLEENGFNVEVYLDYASD
                     LAEIITLIYAKESYTFFSGNLRKETLPKALKATKVNFNEIQVYETTLTPQKVKTPVDG
                     ILFFSPSAIESYLKENKIKNEICFCIGETTAETLEKTTRNIIVADQPSVDNLIEDVIE
                     EYK"
     gene            3628..4656
                     /gene="hemE"
                     /locus_tag="PLQ70_08955"
     CDS             3628..4656
                     /gene="hemE"
                     /locus_tag="PLQ70_08955"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HQE34782.1"
                     /translation="MIKNDLFLKALKGETVERPPVWMMRQAGRYLPEFRALRDKYDFF
                     TRCETPELAAEITVQPIRIVGPDAAILFSDILVVPRAMGIHVELKDNLGPIIPNPIRT
                     MEQVNQVYVPDVNETLGYVFDAVKLTKEMLNDEVPLIGFAGSPWTIFCYAVEGKGSKS
                     FDTAKGFCFSNPVAAHTLLQKITDTTILYLKEKVKAGCNAIQIFDSWGGMLSPTDYQE
                     FSWKYINQIVDALAEITPVIVFGKGCWFALGEMGKSKASALGVDWTCTPRNARYLSGG
                     NVTLQGNFDPSRLLSPIPTIKKMVHEMINEFGKDKYIANLGHGILPNIPVDHAKAFVD
                     AVKEYKQK"
     gene            4703..5338
                     /locus_tag="PLQ70_08960"
     CDS             4703..5338
                     /locus_tag="PLQ70_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34783.1"
                     /translation="MFDNVAPRITPTRFEKNLAGQHNEIVETMKLEVLEKDGYYHIYN
                     RGINGCLIFENDENKSFFLKQFSKYLLGKISIFAYCLMDNHFHLVIRLNDEEKIVTQA
                     FSNFFNSYVKAFNKDIDRTGSLFEKHFKRIKLNDEEYLKQLILYVHLNPKHHLDLKFE
                     DYKYSSYQAFISNKETKIEREEVLKLFGGLENFIFCHNQRNDFLTEKHTLE"
     gene            5456..>5808
                     /locus_tag="PLQ70_08965"
     CDS             5456..>5808
                     /locus_tag="PLQ70_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34784.1"
                     /translation="MLNKGLRDQESIRIDNILKMLHSLTFVPKFWNLEDLLHLENELK
                     DLGMNVESLTDFSQEQLISHLERLHFDWNHFEIFADFLIAFSKESQFDFSGKAIAIYD
                     YVQQESKVFSFGIFN"
BASE COUNT         1993 a          913 c         1057 g         1745 t
ORIGIN      
        1 gccatcaaat atttctttgg ggtggtgcca aatttctttt tgaaagccgc gataaaatga
       61 cttcctgtgc tgtagccaat tttcaaacct acttcattga cattatacga accactgtcc
      121 aataattttc gggcagaatc cattttgtaa tcgaaaagaa aaccataaac ggtgtctcca
      181 taaatttgtt tgaaacccat ttttagcttc ttcaaagtta atcctatttc atcggcaagt
      241 tcctgtaaac ttggtggttc ggcgatatta gcaagaacga gttctttggc tttttttatt
      301 ttcaaaacgt tttcttcatc aatcaaaaac ggacattgct ctgcatttgg atcttcggtt
      361 cggttgaaat acaaacttaa taattcgtat cctttgcctt tataatacaa gttttttatg
      421 gatggatgca agttataatg aaacaattga gtcaaaacaa tcgccatcga cggactgata
      481 tcgccttctt tataatattt tttatcccga ttgtcttcac tcaaaaaagt gatgtaattg
      541 gcttctgaag aaaataaatt atgaaatttt tggatcgaaa tgataaccga aatcacccaa
      601 gaattagggg caatttctaa gtttaaaggc aattcttttt gtggattgta cagcaacaaa
      661 gacttttctt cctttaagtc taaagcgtag cttccttgat tgaaaagaaa tttggctttt
      721 cctttcaaac caaaatggaa ttgaatcaat ccgcttttta cttcacgctg tgtacaatac
      781 acttcatcac tgtcattttg aaaccttagt agggtaaagt cgtcttcgat tgttataata
      841 tcctgtgaac tcattgcgac atttttttaa aataatatga taattatcag gtaattgtta
      901 ataaataaat tttacatttg tcttataaat gcctgatttc attacaaaaa tagtgttttt
      961 aaattaaatt tcagacttat aaacaaaaat catttggcga tactattagt actttgagcg
     1021 ttgttttaat aaaaactgta gttctaactt tgccaatccg attagaaaac atcaaatatg
     1081 gaaaacctaa acatacccaa acacaaaact ttctactcta tagggttgag ttacaagaaa
     1141 gcagatgccg aagttagagg taagtttagt cttgatgcta aaacaaaaac caaagtattg
     1201 ctccaagcca aagaagaagg tatcgaaagt ttaatcgtta cttctacttg caatcgcacc
     1261 gaaatttatg gttatgctga tcatcctttt caactaataa aattaatctg cgaaaacagc
     1321 aatggaactg tcgaagaatt tcaaaaagca ggttttgtat ataaaaacca agaagctatc
     1381 gaccatatgt ttcgagtagg aacaggttta gacagtcaaa ttcttggcga ttttgaaatt
     1441 attggacaaa taaaaaacag ttttgaagaa gctcgttcct tgggattaat caacgccttt
     1501 ttagatagat tgatcaatac ggttattcaa accagcaaaa gaattaaaaa cgaaaccgaa
     1561 attagttcgg gtgctacttc tgtttctttt gcagctgtac agtacatcat tagaaatatt
     1621 gaagatattg gaaataaaaa tattttgctt ttcggaacag gagaaatagg tcgaaatact
     1681 tgcgaaaatt tagtaaaaca cactaaaaac gagcaaatta ccttaattaa tagaaccaaa
     1741 agttctgccg aaattttggc tggcaaactg aatttagtcg taaaagatta ttcagattta
     1801 catctcgaat tacaaaaagc cgatgttttg gtagtggcaa ctggtgcaca gaatccaact
     1861 atcgataaaa ctttgttgaa tttaaaaaag gaccttttgg tcttggattt gtctattcca
     1921 aaaaatgtga atgctgatgt acaacaaatt cctggtgtaa cattaattca tatggattat
     1981 ttgtcccaaa tgacagatga cacgctcgaa agaagaaaac aacacatccc tgctgctgaa
     2041 gccattatcg aggatatgaa attggaattg aatacttgga tgaatggtcg caaatatgcg
     2101 ccaactattc acgcattgaa agcaaaattg aatgatattg ttgctggaga attgactttt
     2161 caacgtaaaa aattatccaa tttcgatgaa gaacaagccg aattaatcac ttctagaatt
     2221 atccaaaaaa ttaccaacca attcgccagt cacctcaaag acgaaaacac ttcggttgac
     2281 gagagtatag agtttatcga aaaagtattt caaataggac tacaaactcc nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaatgcaaaa actaaccgaa agcgattgga
     2461 gcggagctgt ttttgcagct gcaggtttag aaagaatcaa cttaaaacct accgatttca
     2521 tcaatctgga ttggatgatt ccagcaccag ctcaaggtgc aatggttgtt gtggcaatgg
     2581 gtaacgacga atttgttctc gatgctgttt ctcaactcaa tgatgtagaa accgaaattt
     2641 gtacttacat cgaaagacaa ttcctgaaaa cactcgaagg cggttgtacc gcacccattg
     2701 gcgcattggc aaaatattgc gaattggaag acacttttac tttcaaaggt gctttgctgt
     2761 ctattgatgg aaagcaaaaa ctggaaattg aaaaaatcgt acccgttgaa gaatggaaaa
     2821 aattaggttt caattgcgcc aaagaaattt tggaaaacgg tggagcagag ttgatgaaaa
     2881 ccattaagga aagtttgaag aaataatgag taacccaatc agcattctat ctacaaaaat
     2941 acttttgagc catcaaaagc aagccttatt gaatgcaggt ttttcagtaa ccgaagccaa
     3001 tttcatcaaa accgaaaact cggattttga cttaaaagga atcaatgata atttaatttt
     3061 cactagtcaa aatgcggctc aaagcgtttt acttcatccc aaatgcggcg aattaaaatc
     3121 caaaaacgtg ttttgtgtgg gaatgaaaac caaagccttg ttggaagaaa atggttttaa
     3181 tgtggaggta tatttagatt atgcttctga tttggcggaa atcattactt tgatttatgc
     3241 caaagaaagt tatacttttt tcagtgggaa tttgcgaaaa gaaactttac caaaagcact
     3301 gaaagcaacc aaagtcaatt tcaacgaaat tcaagtgtat gaaactactt taacaccgca
     3361 aaaagtaaaa acgccagttg atggaatcct gtttttcagt ccgtcagcca ttgaaagtta
     3421 tttgaaggaa aacaaaataa aaaacgaaat ttgcttttgc attggcgaaa ccactgccga
     3481 aactttggaa aaaacaacca gaaatataat agtcgcggat caaccatcgg ttgataactt
     3541 gattgaggat gtaatcgaag aatataaata aaaacaaacc ctttcagagt tcaaaactct
     3601 gaaagggtta aatgatacaa aagaaaaatg ataaagaacg acctattttt aaaagcatta
     3661 aaaggagaaa ctgtagaacg tccaccagtt tggatgatgc gtcaagcggg aagatatttg
     3721 ccagaattta gagcattgcg tgataaatat gattttttta ctcgttgtga aactccggaa
     3781 ttggctgctg aaatcacggt gcaacccatt cgtattgttg ggccagatgc cgcaattttg
     3841 ttttcggata ttttggtagt gccaagagca atgggaattc acgtggaatt gaaagacaat
     3901 ttggggccga ttattccaaa tccaattcgc acaatggagc aagtaaatca agtttatgtt
     3961 ccagacgtaa acgaaacttt aggctacgtt tttgacgctg tgaaattgac caaagaaatg
     4021 ttgaacgatg aggtgccttt gataggtttt gccggttcgc cttggactat tttttgttat
     4081 gccgtggaag gaaaaggctc gaaaagtttt gacactgcca aaggattttg tttctcgaac
     4141 cctgttgcag cgcatacttt attgcaaaaa attacagata cgactatttt atatttgaaa
     4201 gaaaaagtaa aagcaggttg caacgccatt cagattttcg attcttgggg cggaatgctt
     4261 tcaccaacag attatcagga attctcctgg aaatacatca accaaatcgt ggatgctttg
     4321 gcagagatta cgccagtgat tgttttcgga aaaggatgtt ggtttgccct tggtgaaatg
     4381 ggaaaaagta aggcttcggc attgggagta gattggactt gtacaccaag aaatgctcgt
     4441 tatttgtctg gcggaaacgt gactttacaa ggaaatttcg atccgtcaag attattgtcg
     4501 ccaattccaa ccatcaagaa aatggtgcac gaaatgatta acgaattcgg aaaagacaaa
     4561 tacatcgcca atttaggtca cggaatttta ccaaatattc ccgtggatca cgccaaagct
     4621 tttgtggatg cggtgaagga atataagcag aaataggagg tgtacagtat atcctgcaag
     4681 gttttttttt aaccttgtag gtatgtttga taatgtcgcc ccaagaataa cacctacaag
     4741 gtttgaaaaa aaccttgcag gacaacataa tgaaatagtt gaaactatga aattagaagt
     4801 tttagaaaag gatggttatt atcatattta caacagagga ataaatggct gtttaatttt
     4861 tgaaaatgat gaaaacaaaa gcttcttttt gaaacagttt tcaaaatatt tattgggtaa
     4921 aatttcaata tttgcatatt gcttgatgga taatcatttt catttggtta tccgattaaa
     4981 tgatgaggaa aaaatagtaa cacaggcttt ttctaatttt tttaattcct atgttaaagc
     5041 ttttaataaa gatattgata gaacgggaag tctttttgaa aaacacttca agagaataaa
     5101 actcaatgat gaagagtatt tgaagcaatt gatactgtat gtacatttga atccaaaaca
     5161 tcatttagat ttaaagtttg aggactataa atattcatct tatcaggctt tcatttcaaa
     5221 taaagaaaca aaaattgaga gagaagaggt gttgaaatta tttggaggtt tagaaaattt
     5281 cattttttgc cataatcaaa gaaatgattt cttaacagaa aaacatacat tggaatgatt
     5341 tcaatttcct gcaaggtttt tttcaaacct tgtaggtatg tttgaaaacg agacccaaaa
     5401 agcaatacct acaaggtttg aaaaaaacct tgcaggataa cacacaataa ttaaaatgtt
     5461 aaacaaaggc ttacgagatc aggaaagtat tcgaatagac aacattctaa agatgttgca
     5521 ttcgctgact tttgtgccca agttttggaa tttagaagat ttgctacatc tcgaaaatga
     5581 attgaaagat ttaggaatga atgtcgaaag tcttacagat tttagccaag agcagttaat
     5641 ttcccatttg gaacgtcttc attttgattg gaatcatttc gagatatttg ccgatttttt
     5701 aatcgcgttt tcgaaagaaa gccaatttga tttttctgga aaagccattg ctatttacga
     5761 ttatgttcaa caagaaagta aggtcttttc gtttggaata tttaataa
//
LOCUS       DAOSNN010000172         5798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13967_length_5798_cov_5.885426, whole genome shotgun sequence.
ACCESSION   DAOSNN010000172 DAOSNN010000000
VERSION     DAOSNN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13967_length_5798_cov_5.885426"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PLQ70_08970"
     CDS             <1..1137
                     /locus_tag="PLQ70_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQE34785.1"
                     /translation="AENIDVKSLYAKLEKGKSILMEYFSGFERMYIFTLANQRIKLEH
                     INITDTAIPKIISFLDYFSDANKIIDDISGYNHYGNSIYKMLQLPTNWTYQNLVIIPD
                     GILCFLPFEALITKESTTTNFAQMHYLLNDFRIAYNNSATFYLNGKTASSDKKSVLGI
                     FPVFEKTNYELTFSKKEMQSIKNDFDGKYFKNSNATFDNFKMNAPNYSILHLSTHATS
                     GDTETPASIKFYDREILYSELYNLNISTDLVVLSACETGIGKLYKSEGAMSVARGFQF
                     AGAQNLLFSLWKVNDYTTSVFMADFYANIKNGSSYLEANHQAKLDFLNDKSVSNAKKS
                     PYYWSAFVYYGGIETAEKPTNYSFYILGLLLLIGLFLVYKHFRK"
     gene            1134..1574
                     /locus_tag="PLQ70_08975"
     CDS             1134..1574
                     /locus_tag="PLQ70_08975"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HQE34786.1"
                     /translation="MNYLPYLLKKENYKKIKFKVSKTQHLLIKAKINGVKGNFILDTG
                     ASNSCIGFESVELFQLEAKDSKTKASGAGATGMTTQTAHKNKLQLGNWKYSDFNLVIF
                     DLSHVNEALIAYKAKPVHGIIGADILMKGKGIIDYYNHCLYLGK"
     gene            complement(1626..2852)
                     /gene="odhB"
                     /locus_tag="PLQ70_08980"
     CDS             complement(1626..2852)
                     /gene="odhB"
                     /locus_tag="PLQ70_08980"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HQE34787.1"
                     /translation="MILEMKVPSPGESIKEVEIATWLVKEGDYVEKDQAIAEVDSDKA
                     TLELPAEASGIISLKAEEGDTVAVGAIVCHIDTDAAKPSAAAPVAEVKKEEKAAEVKK
                     EEVKVVAPVAPTSYASGTPSPAARKILDEKNIQPSTIVGTGKDGRITKDDAINAVPSM
                     GTPTGGNRGSERTKLSMLRRKVAERLVAAKNDTAMLTTFNEVNMTPINTLRNEYKDAF
                     KAKHGGLGLGYMSFFTKAVTRALELFPDVNSMMDGDYKVAYDFADISIAVSGPKGLMV
                     PVVRNAENLSFRGIEAEIKRLAIKARDGQITVDDMTGGTFTITNGGVFGSMLSTPIIN
                     PPQSGILGMHNIIERPIAVNGKVEIHPMMYVALSYDHRIIDGRESVGFLVAVKEALEN
                     PVELLCDGNPKKAFEL"
     gene            complement(2902..5679)
                     /locus_tag="PLQ70_08985"
     CDS             complement(2902..5679)
                     /locus_tag="PLQ70_08985"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HQE34788.1"
                     /translation="MDRFSFLNAAHTEFFAQLYDQYLENPDSVEPSWRSFFQGFDFGM
                     TTYNEENPVTQPEIKTVVAPENNSGTEKLLKEFKVIKLIDAYRSRGHLFTRTNPVHKR
                     RTYSPTLDLENFGLTKADLPIVFDAARIIKIEPCSLAEILGHLDKIYCQSIGVEYMYI
                     RNPNDIQWIQSKIGHNDNQPDFSVEQKKTILRKLNKAVAFENFLHTKYVGQKRFSLEG
                     GESIIPALDALIEQAAEKGVEHFVMGMAHRGRLNVLANIFGKSTQDIFSEFDGKDYQD
                     QEYFDGDVKYHLGLSAEKRTSTGKTININLAPNPSHLETVGAVIEGITRAKQDKYFPN
                     DFSKVLPIAVHGDAAIAGQGLLYEIIQMSQLDGYKTGGTIHIVINNQVGFTTNYLDAR
                     SSTYCTDIAKVTLSPVLHVNADDAEAVVHAVQFALDFRMHFGRDVFIDLLGYRKYGHN
                     EGDEPRFTQPILYKIIAKHKNPRDIYAEKLLAEGVIDATYVKELETEYKADLEVSLEE
                     SRKKELTIITPFLQNEWKGFETAGISQMLTKVNTTYPKEGLTAVANAICNLPTDKKFI
                     SKVQKLINDRKTMFFETNKLDWGMAEHLAYGSLLREGFDVRISGQDVERGTFSHRHAV
                     VKVEDSEEEVVLINSLEGKKGKFNAFNSLLSEYGVLGFDYGYALANPNALTIWEAQFG
                     DFSNGAQIMIDQYISCGEDKWNNHNGIVLLLPHGYEGQGAEHSSGRMERFLQLCARQN
                     MYVADCTTPANFFHLLRRQMKLKFRKPLVVFTPKSLLRDPRVVSSIEELENGAFQETI
                     DDNSIVKSEIKTLVFCTGKFYYDITAEREIRGRKDVAVVRIEQLFPFPAEQLKEIIAK
                     YPNADDYVWAQEEPKNMGAYGYMLMNFDLVKWRLASLRVSSASASGSYTRSKNRHAKA
                     LKMVFEKDYIR"
BASE COUNT         1712 a         1111 c         1012 g         1963 t
ORIGIN      
        1 gccgaaaata tagatgtaaa atccttgtat gctaaactag aaaaaggcaa atcgatattg
       61 atggaatatt tttctgggtt tgagagaatg tatattttca ccttggcaaa ccaaagaata
      121 aagcttgaac acattaacat aacagacacg gcgataccga aaataatttc gtttttggat
      181 tatttcagcg atgccaataa aataattgac gatatttcgg gttacaatca ttatggtaat
      241 tcaatttaca aaatgttgca attacctaca aactggactt atcaaaacct cgtcataatc
      301 ccagacggaa tactgtgttt tcttcctttc gaagccttga ttaccaaaga atccaccacg
      361 accaattttg cccaaatgca ttatttgctg aatgatttca ggattgccta taataattcg
      421 gcaacatttt atttgaatgg aaaaaccgct tccagtgaca agaaatcagt ccttggcatt
      481 tttcccgttt tcgaaaaaac gaattacgaa ctgacatttt ccaaaaaaga aatgcaatcg
      541 ataaaaaatg atttcgacgg gaaatatttc aaaaattcga atgccacttt tgataatttc
      601 aaaatgaatg cgcccaatta ttcgattttg catttgagca cgcacgctac ttccggcgac
      661 actgaaacac ccgcaagtat aaagttttat gatcgggaaa tcctgtattc ggaattgtac
      721 aacctcaaca tcagtaccga tttggtggtt ttgagtgcct gcgaaaccgg aatcggaaaa
      781 ctctacaaat ccgaaggtgc gatgagcgtt gcccgaggat tccagtttgc cggagcacaa
      841 aatctattgt tttcgttgtg gaaagtcaac gattacacga cttcggtttt tatggccgat
      901 ttttatgcaa acatcaaaaa cggaagttcg tatttggaag ccaatcatca agccaaacta
      961 gatttcttga acgacaaatc ggtttcgaat gccaaaaaat cgccttatta ctggagtgct
     1021 ttcgtgtatt atggcggaat tgaaactgcc gagaaaccaa cgaattattc tttttatatt
     1081 cttgggttat tgctattaat tggactattt ttggtctata aacactttcg aaaatgaact
     1141 accttcctta tcttctcaaa aaagaaaact acaaaaaaat aaaattcaag gtttcgaaaa
     1201 cccaacattt gctgataaaa gcaaagatta acggcgtaaa aggcaatttt attctcgaca
     1261 cgggagcatc caatagttgt attggttttg aaagtgtcga attgtttcaa ctcgaagcca
     1321 aagattctaa aactaaagct tctggtgctg gagcaaccgg aatgaccact caaacagcgc
     1381 ataaaaataa actacaattg ggcaattgga aatacagtga ttttaatctt gttatttttg
     1441 atttatccca cgtcaatgaa gcgttgattg catataaagc taaacctgtt cacggcatca
     1501 taggtgccga tattttaatg aaaggcaagg gaattattga ttactacaat cattgtttgt
     1561 atttggggaa ataaaaaatc ccgttcaatt tctcgaacgg gatttctact atttgaaata
     1621 gaattttaca attcaaatgc ttttttagga tttccatcgc aaagcaattc tactggattt
     1681 tctaaagctt ctttaaccgc tactaagaaa ccaacagact cacgaccatc gataattctg
     1741 tggtcataag aaagagctac atacatcatt gggtgaattt ccactttgcc atttacagca
     1801 atcggacgct ctataatgtt gtgcattcca aggattccag attgaggagg gttgataatt
     1861 ggagttgaca acatagatcc aaaaacacca ccatttgtga tagtaaatgt tcctcctgtc
     1921 atatcatcaa cggtgatttg tccgtcacgt gctttgatgg ccaatctttt gatttcagct
     1981 tcgattcctc tgaaagataa attttcggca ttacgaacca caggaaccat taatcctttt
     2041 ggtccagaaa cggcgataga gatatcagca aaatcgtaag ctactttgta atcaccgtcc
     2101 atcattgagt ttacatcagg gaacaattct aaagctcttg ttaccgcttt tgtaaaaaat
     2161 gacatatatc ctaaaccaag accgccgtgt ttggctttga acgcatcttt gtattcgtta
     2221 cgcaaagtat tgattggtgt catgtttact tcgttgaacg ttgtcaacat cgcagtatca
     2281 ttcttagcag ccactaatct ttcggctact ttacgacgca acattgataa ttttgtacgt
     2341 tctgaaccac gatttcctcc cgtaggggtt cccatagatg gaactgcgtt tatggcatca
     2401 tctttagtga ttcttccgtc ttttccggtt ccaacaatag tagaaggttg aatatttttt
     2461 tcgtctaata tttttctcgc tgctggcgat ggagttcctg aagcgtaaga agttggtgct
     2521 actggagcaa ctacttttac ttcttctttc ttaacttcgg cagctttttc ttcttttttc
     2581 acttctgcta ctggcgctgc agctgatggt tttgctgcat cagtatcgat atgacacaca
     2641 attgctccaa cggctacagt atctccttct tctgctttca aagagatgat tccgctagct
     2701 tctgctggca attctaatgt tgctttatca gaatctactt cggcaatggc ttgatctttt
     2761 tctacataat cgccctcttt tactaaccaa gttgcaattt caacttcttt tattgattcc
     2821 cctggtgatg ggactttcat ttctaaaatc atcttgtttg tattaaatta tgaattttaa
     2881 attttttata ggaactgttt tttatctaat ataatccttt tcgaaaacca ttttaagggc
     2941 ttttgcatga cggtttttag atcttgtata acttcctgac gctgatgctg aggaaactct
     3001 taatgatgcc aatctccatt ttactaaatc gaaattcatc aacatataac cgtaagcccc
     3061 catgttttta ggctcttctt gtgcccaaac ataatcgtcg gcatttgggt attttgcaat
     3121 aatttctttt agttgttcgg cagggaatgg gaacaattgt tcgattctca caactgctac
     3181 atcctttcta cctcttattt ctctttcggc tgtgatgtca taatagaatt ttcctgtaca
     3241 gaaaaccaga gttttaatct ctgatttgac tatagaattg tcatcaatag tttcttggaa
     3301 tgcaccattt tctaattctt cgatgctaga aactactctt gggtcacgaa gcaaactttt
     3361 tggtgtgaaa acaacaagtg gtttacggaa ttttaatttc atttgtcttc tcaataaatg
     3421 gaagaagttg gccggtgttg tacaatcggc aacatacata ttttgtctgg cgcaaagttg
     3481 caaaaagcgt tccatacgtc ctgaggaatg ctcagctcct tgtccttcat aaccgtgagg
     3541 caataataaa acgattccgt tatgattgtt ccatttgtct tctccacacg aaatatattg
     3601 gtcaatcatg atttgggctc cgttggagaa atccccaaat tgggcttccc aaatggttag
     3661 tgcatttgga tttgccaagg catatccata atcaaaacct aaaactccgt attcagacag
     3721 taatgaattg aaagcgttaa attttccttt ttttccttca aggctattga ttaaaactac
     3781 ttcttcttct gaatcttcaa cttttacaac tgcatgacga tgtgaaaaag ttccgcgttc
     3841 tacgtcttgt cctgaaatac gaacgtcaaa accttctctt agcaaagagc cgtaggctaa
     3901 atgctcggcc attccccaat ctagtttatt ggtttcgaaa aacattgttt ttctgtcgtt
     3961 gattaatttt tgaactttgc taatgaattt tttatcggtt ggcaaattac aaatggcatt
     4021 ggctacagcc gtaagacctt cctttggata agtcgtgtta acttttgtaa gcatttgtga
     4081 aattccagca gtttcaaaac ctttccactc attttgtaaa aacggagtga tgatagtcaa
     4141 ttcttttttg cgagattctt caaggctaac ctctaaatcg gctttgtatt cggtttctaa
     4201 ttcttttaca taagtagcat cgattacgcc ttcggccaat aatttttcag cataaatatc
     4261 tcttggattt ttatgtttgg cgatgatttt atataaaata ggttgtgtaa aacggggttc
     4321 gtcaccttcg ttatgaccgt attttctata gcccaacaaa tcgataaata cgtcacgacc
     4381 aaaatgcatt ctgaaatcta aagcgaattg tacagcatgt acaacagctt cggcatcatc
     4441 ggcattgacg tgtaatactg gcgaaagtgt tactttggca atatctgtac aataagttga
     4501 tgaacgtgcg tcaagataat tagtagtaaa accaacttga ttattgatta caatatgaat
     4561 agttccacct gttttataac catctaattg cgacatttga atgatttcat ataaaagacc
     4621 ttgtccagca atagcggcat caccgtgaac ggcaattggc aatacttttg agaaatcgtt
     4681 cgggaagtat ttgtcttgtt tggctcttgt tattccttcg attacagctc caacggtttc
     4741 taaatgcgat ggatttggtg ctaaattgat attaatggtt tttcctgtac tggttctttt
     4801 ttcggcactt aatcccaaat ggtattttac gtcaccatca aaatattctt ggtcttggta
     4861 atcttttccg tcaaattcgc taaaaatatc ttgggtagat tttccaaaaa tgtttgccaa
     4921 aacattcaaa cgaccacggt gagccattcc cattacaaaa tgttcgactc ctttttcggc
     4981 tgcttgttca atcaaagcgt caagtgctgg aatgattgat tctcctcctt cgagtgaaaa
     5041 acgtttttga ccaacatact tagtgtgcaa gaagttttcg aatgcaacgg ctttgtttaa
     5101 ttttcttaga attgtttttt tctgttctac cgaaaaatct ggctggttgt cgttgtgtcc
     5161 tattttagat tgaatccatt gaatatcatt aggatttcgg atgtacatat attctacacc
     5221 aatagattgg cagtagattt tatctaaatg acctaatatt tcggccaaac tgcaaggctc
     5281 aattttgatg attctagcag catcaaaaac gattggtaaa tcggcttttg ttaacccaaa
     5341 attctccaag tctaatgttg gcgaataagt tcttctttta tgaaccggat ttgttctagt
     5401 aaacaaatgt ccacgacttc tataagcatc aattaatttg ataaccttaa actctttcag
     5461 caatttttct gttccagaat tgttttcagg agctacaact gtcttgattt ctggttgagt
     5521 tactggattt tcttcgttgt aagtggtcat tccaaagtcg aaaccttgaa aaaaacttct
     5581 ccaacttggc tctacgctat ctggattttc taaatattga tcgtaaagtt gggcaaaaaa
     5641 ttcggtgtga gccgcgttta agaatgaaaa cctgtccata attgtataaa atatctttat
     5701 atgttaaaaa tagtttggca aaagtacaat aaaacaatac acataatatt tttttttacg
     5761 tacttttacg taataatttg ttaaattaat ccaaaaag
//
LOCUS       DAOSNN010000173         5794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_13980_length_5794_cov_4.900157, whole genome shotgun sequence.
ACCESSION   DAOSNN010000173 DAOSNN010000000
VERSION     DAOSNN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13980_length_5794_cov_4.900157"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLQ70_08990"
     CDS             <1..500
                     /locus_tag="PLQ70_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34789.1"
                     /translation="LIYLIMDSEWYLYNSSMITNAQLEFDSNEEINANQPNIKLPEWF
                     ADKLITDFFKYEEESLEQHWVEKGLAVNETAIEFYDTEIKPILYSEQHQDLVKDYKML
                     VGDVFNFETIFRDNNWNYEMNNDFQIKHQEIISKYRTIYSLNLDKWKKALASLNEIIL
                     QKNIK"
     gene            complement(507..1358)
                     /locus_tag="PLQ70_08995"
     CDS             complement(507..1358)
                     /locus_tag="PLQ70_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HQE34790.1"
                     /translation="MVFLILSIICSVSVGVIFKIARNYSINIPQIIAANYLIALSLCY
                     FFFRPDFAEIGEEIPWKIYFSLGVLMPTIFLFLAASIKNVGIVKTDAAQRLSLFIPIL
                     AAWLLFDEKFSTLKIAAFGIAFPALLFILNKPSEKTSNKWVYPAIVLIGFGIIDILFK
                     QIALFTDLRYTTSLFIVFGLALPIMLVVVGYEMGFKKVKFDYKNLLFGSLVGIFNFGN
                     ILFYLKAHQSFAENPSTVFAGMNMGVILIGSLTGIFIFKEKLSKMNYAGLFLALIAIV
                     LIVLSQQ"
     gene            complement(1399..1782)
                     /locus_tag="PLQ70_09000"
     CDS             complement(1399..1782)
                     /locus_tag="PLQ70_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HQE34791.1"
                     /translation="MNNSVHCFCGSTISFEDCCQKYINGSQKAPTALALMKSRYSAYA
                     THQADYLFDTTHISQRKYYSKADILNWATANEWQKLEIISFTETTVEFKAYFLDKNKT
                     AQVHYEFSTFKQENGSWFYVDGKFE"
     gene            complement(1775..2005)
                     /locus_tag="PLQ70_09005"
     CDS             complement(1775..2005)
                     /locus_tag="PLQ70_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34792.1"
                     /translation="MEKSQFEELKNGVQEIIDFIAAKDNKSANNKLLEVSDKLDDFLD
                     HTDEDEDLIEISKYQVLLNQLFKKINPEEEGE"
     gene            complement(2008..2160)
                     /locus_tag="PLQ70_09010"
     CDS             complement(2008..2160)
                     /locus_tag="PLQ70_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34793.1"
                     /translation="MGRNNERNIKIHNDKLHKEQQKVKAAKELRKEKLKQIVKKFNED
                     KSSENN"
     gene            complement(2160..2702)
                     /locus_tag="PLQ70_09015"
     CDS             complement(2160..2702)
                     /locus_tag="PLQ70_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34794.1"
                     /translation="MNKVFRPSPNSFKNTFCVFQELLPEKVQGMPIQYDSKSGSKYFY
                     TEEGMYRLSNHWGRLANSKWRLEPMEPETASKTKLGFAAWSEFYPDNAEEKIYYLEAN
                     FIKKTVNYQHKNNPNYDKKAILRTSFETTKKIKQIRNLFALTSWAKYYEYDDLDELRA
                     TIINQLIFTNKTLEEIKREL"
     gene            2795..3043
                     /locus_tag="PLQ70_09020"
     CDS             2795..3043
                     /locus_tag="PLQ70_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34795.1"
                     /translation="MTKSSQEKMLQKGVFTGIIEQDENNNFFCGDYLLDYKMVMANFK
                     LGDRVTIKSVIENPSDINYNTYPKKSKNFDKANNKPQQ"
     gene            3174..3614
                     /locus_tag="PLQ70_09025"
     CDS             3174..3614
                     /locus_tag="PLQ70_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HQE34796.1"
                     /translation="MKKIVEYRKLLNVDKTVELKELKTIYRNAMKESHPDKFQGDEAG
                     LKAAEENSTKIIEAYHFLVSINPETIKANLPEYTETISTATITDYKFVEGRLIINFSN
                     GSVYEYISVPKATYVKMVNADSPGRFAKRHILNAFTWRKTINQD"
     gene            3757..3996
                     /locus_tag="PLQ70_09030"
     CDS             3757..3996
                     /locus_tag="PLQ70_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34797.1"
                     /translation="MKDPCNLYISQRNKAKAVLDILEKQRAEINFKLKSNDFCANLHK
                     ELRNLNMDIRITLNEIEHAEYNIQECISKNSPISN"
     gene            4173..4940
                     /locus_tag="PLQ70_09035"
     CDS             4173..4940
                     /locus_tag="PLQ70_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HQE34798.1"
                     /translation="MKKIILLLSATVLLISCSKTKYTISGTAKGIENGKTVIMETQDA
                     SGMSLIAVDTVKVENGKFEITGKATEPSFHMINVEGVQGKFPFILENGDISIEINKDS
                     INKSKVSGTYNNDEFVSFNEELIKIQKEIMAFQKKNTPLMSQAQQSNDTATINKLMAE
                     YNKLQEVVTTESKKKYNSYTESHPKSFISVLIVQGMTNDPSVDIKAVEKLYNGLDESL
                     KTTKPGKAISQKIKAAKTPSVGAGAMPPAQAAPGTAK"
     gene            4959..5345
                     /locus_tag="PLQ70_09040"
                     /pseudo
     CDS             4959..5345
                     /locus_tag="PLQ70_09040"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
     gene            5510..5590
                     /locus_tag="PLQ70_09045"
     tRNA            5510..5590
                     /locus_tag="PLQ70_09045"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5544..5546,aa:Tyr,seq:gta)
BASE COUNT         2099 a          934 c          893 g         1868 t
ORIGIN      
        1 aactcatcta tttaattatg gattcagaat ggtatttata caattcatcg atgattacta
       61 atgctcaatt agagtttgat tctaatgaag aaataaatgc aaatcaaccc aatattaaac
      121 ttccagaatg gtttgcagac aaacttatca ctgatttttt taaatatgaa gaagaatctc
      181 tagaacaaca ttgggtagaa aaaggattgg ctgtaaacga aactgccata gaattttatg
      241 ataccgaaat caaacccatt ttatattctg aacagcatca agatttagtt aaagattata
      301 aaatgttggt gggagatgtt ttcaatttcg aaaccatttt tagagataac aattggaatt
      361 atgaaatgaa taatgacttt caaatcaaac atcaggaaat tatttcaaaa taccgcacga
      421 tttattcctt gaatcttgat aaatggaaaa aagcattggc atctttaaac gaaattatac
      481 ttcaaaaaaa tatcaaataa ttggaattat tgttgtgata acacaattaa tacaattgca
      541 atcaaagcca aaaataatcc cgcataattc attttggaca gtttttcttt aaaaatgaag
      601 atacctgtta ggctcccaat gagaattact cccatattca ttcccgcaaa aactgtcgaa
      661 ggattttcgg caaaagattg atgtgctttt agataaaaca gaatattgcc aaagttgaaa
      721 ataccgacca aacttccaaa aagaaggttt ttataatcga attttacttt tttgaacccc
      781 atttcataac caaccaccac cagcattatc ggcaaagcca agccaaaaac gataaacaaa
      841 gaagttgtgt atcgcaaatc tgtgaacaag gcaatttgtt taaaaagtat atcaataatt
      901 ccaaatccaa ttaaaacaat ggcaggataa acccatttgt ttgatgtctt ttctgaaggt
      961 ttgttcaaaa tgaaaagaag tgcaggaaaa gcaatcccga aagctgctat ttttaaagtg
     1021 ctgaattttt cgtcgaaaag taaccaagcc gccaaaatag gaatgaacaa cgaaagtcgt
     1081 tgagccgcat cggtttttac gattcccaca tttttaatcg aagccgctaa aaaaagaaaa
     1141 atagtaggca ttaaaacgcc aagagaaaaa tagatcttcc aaggaatctc ttctccaatt
     1201 tcagcaaaat ctggacgaaa aaaaaagtag caaagggaaa gcgctattaa atagtttgcg
     1261 gcaataatct gaggaatatt aatggaatag ttgcgagcta ttttaaaaat aacacccaca
     1321 ctcacgctgc aaataatact taaaatcagg aataccataa tttatttttt cttaagaagt
     1381 gttcttgaac tctaatgttt attcaaactt tccatctaca taaaaccaac tgccattttc
     1441 ctgcttgaag gtagaaaact cataatgtac ttgggcagtt ttatttttat ccagaaaata
     1501 ggctttgaat tccaccgtag tttctgtaaa ggaaataatt tctagttttt gccattcatt
     1561 ggctgtagcc caattcaaaa tatcggcttt agaatagtat tttcgttggg aaatgtgagt
     1621 agtatcaaac aaataatccg cttgatgcgt ggcataagcg gaatatcttg atttcatcaa
     1681 ggctaaagca gtaggtgctt tttggcttcc attgatgtat ttttggcaac aatcttcaaa
     1741 cgaaattgtt gaaccacaaa agcaatgaac tgaattattc accttcttct tctggattga
     1801 tttttttaaa caattgattc aaaagtactt gatatttact gatttcgatt aaatcttcgt
     1861 cttcgtctgt atgatccaaa aaatcgtcta atttatcgct tacttccaat aatttattgt
     1921 tggctgattt attgtctttg gctgcaatga aatctataat ttcttgaacg ccgtttttta
     1981 attcttcgaa ttgacttttt tccataatta attattttct gaagatttat cttcgttgaa
     2041 ttttttcacg atttgtttca gtttttcttt gcgcaactct ttggcagcct taactttctg
     2101 ctgttctttg tgcagtttgt cgttgtgtat tttgatattc ctttcgttat ttcttcccat
     2161 tatagttctc tttttatttc ttccaatgtt ttattggtaa aaattaattg attgattatt
     2221 gttgcacgta attcatccaa atcgtcgtat tcataatatt ttgcccaaga agttaacgca
     2281 aataaattcc gaatttgttt gattttttta gtcgtttcaa aactcgttcg cagaatcgcc
     2341 tttttatcat aattcggatt atttttatgc tgataattaa ccgttttttt gatgaaatta
     2401 gcttctaaat aatagatttt ttcttcggca ttatcgggat aaaattcgct ccaagcagca
     2461 aaacctaatt ttgtttttga tgcagtttct ggttccattg gctccaatcg ccatttgctg
     2521 ttagccaatc ttccccaatg gttcgacaat cgatacattc cttcctcggt ataaaaatac
     2581 ttacttcccg acttgctatc gtattgaatt ggcattcctt gaactttttc aggcagtagt
     2641 tcttgaaata cacaaaaggt gtttttaaaa gaattaggac tcggacgaaa tactttgttc
     2701 atcctgcaaa gataatcaga atgcgcagaa ttaagcaaga caaacaataa ttgattaact
     2761 ttgcgaccaa gaattcactt aatacaaaac gattatgacc aaatcttcac aagaaaaaat
     2821 gcttcaaaaa ggagttttca caggaatcat tgaacaagac gaaaacaata actttttttg
     2881 tggcgactat ttattggatt acaaaatggt aatggccaat tttaaacttg gcgatagggt
     2941 tactatcaaa tcagtgattg aaaacccaag tgatatcaac tacaatacat atcctaaaaa
     3001 gtcaaaaaac tttgacaaag cgaataacaa accgcaacaa taatcagttt taattgattt
     3061 tttttcagtc gaaaattgaa aacttaaaaa aaagtgtacc tttgcaaaaa attgtcaatt
     3121 ttggaactaa attagattga ttccatagta aaagacaagt agttatctta tttatgaaaa
     3181 aaatagttga ataccgcaag ctgctaaatg ttgataaaac agtagaatta aaagaattaa
     3241 aaaccattta tcgcaatgcg atgaaagaat cgcatcctga taaatttcaa ggagacgaag
     3301 ccggtttgaa agctgctgaa gaaaacagta ccaaaattat tgaagcctac cactttttag
     3361 taagcatcaa ccctgaaacg atcaaagcta acttgccaga atatacagaa acaatttcta
     3421 cagcaactat aactgattac aagtttgttg aaggaagatt aattatcaat ttctctaacg
     3481 gtagtgttta tgagtacatt agtgtaccta aagctactta cgtaaaaatg gtaaatgctg
     3541 attctccagg aagatttgca aaaagacaca ttctaaatgc ttttacttgg agaaaaacta
     3601 tcaaccaaga ttaaaacatt ttactagaat atatttagaa acactccttt taggggtgtt
     3661 ttttttgttg ttcatcgaaa aaacaaagca agaattgttt agtggtttta ttttgagtaa
     3721 gttagcagtc ctaaatacta tttcaaatta ctcattatga aagatccttg taatctatat
     3781 atttctcaac gtaacaaagc aaaagcggta cttgatattc tggaaaaaca aagagctgaa
     3841 atcaatttta aactcaaatc gaatgatttc tgtgctaatt tacataagga attaagaaac
     3901 ttgaacatgg atattcgaat tactttgaat gaaatagaac atgctgaata taatattcaa
     3961 gaatgtatct caaaaaacag cccaatttcg aattaagaaa ctctacttac atctcaataa
     4021 tttgattttt tgactaaaac tagtcataaa atcatttgtg cctgaatgag aaatactatt
     4081 tattagttat aacaaaaatt taaggcttaa aattattgat attttataaa attaaatact
     4141 tttgcacact taaatcattt aacttaatca taatgaaaaa aataatttta ttactatctg
     4201 ctactgtgct tttaatttct tgtagcaaaa ccaagtacac tatttcagga actgcaaaag
     4261 gaattgaaaa tggtaagacc gtaattatgg aaacacaaga tgcttcaggt atgagtctta
     4321 ttgccgtaga tacagtaaaa gtagaaaatg gaaaatttga aatcacagga aaagctacag
     4381 aaccttcatt tcatatgata aacgtagaag gagttcaagg aaaatttcct tttatcttag
     4441 aaaatggaga tattagtatt gaaattaata aagatagcat caataaatct aaagtttctg
     4501 gaacatacaa taatgatgag tttgtaagtt ttaacgaaga acttataaaa attcaaaaag
     4561 agataatggc tttccaaaag aaaaacactc cattaatgtc tcaagcacaa caaagtaatg
     4621 acactgctac tatcaataaa ttaatggcag agtataacaa acttcaagaa gttgtaacta
     4681 ctgaatctaa aaagaaatac aatagctata cagaatctca tcctaaatca ttcattagtg
     4741 tattgattgt tcaaggaatg acaaacgatc catcagttga tataaaagct gttgaaaaat
     4801 tatataatgg tcttgatgaa tcattaaaaa caacaaaacc aggtaaagct atctcacaaa
     4861 aaattaaagc cgcaaaaaca ccttcagttg gtgctggagc aatgccacca gctcaagcag
     4921 ctccgggtac agctaaatga agttctgatt tttcagctcc aaatcctgag ggaaaaatag
     4981 ttagtctaaa agaaagttta ggaaagataa ctattgttga tttttgggct tcttggtgcg
     5041 gaccttgtag agctgaaaac cctaatatgg ttgcccttta caaagatttt cattccaaag
     5101 gtttaaatat tattggggta tctctagata aagatgcagc taaatggaaa gaagctattg
     5161 caaaagataa attggtttgg actcaggtat cacatctaaa atattgggac gaacctatag
     5221 ccgcacaata tcaagttcaa gttattcctg ctccttttat actagatgcc tcgggtaaat
     5281 taattgcaaa agatttaaaa ggcgaagcac ttcgagcaaa aatagccgag cttttatcta
     5341 aataacattt aagttttata gaatataaaa aatctcccta gtggagattt ttttatttta
     5401 ttcaatacca acacattaaa aacttatcta aaattaagtt gaaatataat ttggaaacct
     5461 gaaaacagtt gttatatttg caaccgctta ccgctaataa agtggttctg gggagatact
     5521 caagcggcca acgagggcag actgtaaatc tgctgtgtga acttcgcagg ttcgaatcct
     5581 gctctcccca caagaataaa tgacacattt ttaagaagct tgcttcatta aaaatgtgtt
     5641 tttatttttg tgcaaggttt cctcttcaag gatcattgaa aataatccga ctttttttta
     5701 gaaattaaac cattcctagc caatcgtaat taggaaacct tgtccccaca aaaaaagtcc
     5761 tgaaatttca ggactttttt tatttactac agtt
//
LOCUS       DAOSNN010000174         5767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_14067_length_5767_cov_5.356268, whole genome shotgun sequence.
ACCESSION   DAOSNN010000174 DAOSNN010000000
VERSION     DAOSNN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14067_length_5767_cov_5.356268"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLQ70_09050"
     CDS             complement(<1..254)
                     /locus_tag="PLQ70_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQE34799.1"
                     /translation="MLLSNFSNFILESGTDEAGRGCLAGPVTAAAVILPTDFENAILN
                     DSKQLSEKVREKLKPIIEAQAITFAVTHLHPKEIDEINIL"
     gene            424..2376
                     /locus_tag="PLQ70_09055"
     CDS             424..2376
                     /locus_tag="PLQ70_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HQE34800.1"
                     /translation="MRIFFLVVLLFCSTLLFSQEKNSRKPNILDNLIGKDSVKKNEPV
                     ATIDMYRVITLERDTTYIDTSLTIQKKYSHNYLRKDLFGLLAFPNEGQTYNTLQYSLN
                     TFSPLPEFGFKAKHFNFIEANQIRYCSVATPVTEFYFKSVMQKGQNVDAFFTVNTSPR
                     FNFSLAYKGLRSDGKYINQMASTGNFRFTASYNTKNKRYFANTHFTGQDILNEENGGI
                     TDVEAFESGNPDYLTRERLEVYLKDARSFLKGKRVFLDHFFRINSVKGNNNLYVSHQF
                     NYENKFFEYNQPTVTSTVGGTTVYRFGDSYKTSGINDQTHYNKTYNKVGLAYENSTLG
                     KFHFFVDDFFSNYYYNQILILDDQTIPASLSRRIDSYGGQYDYQKNKWTGKFLFSRSF
                     TNQSLSNLDAKMQYELNDETLLSFQYQNINKLPNDNYNLYQSSFVNYNWSNDFKNEKI
                     NSIIVNANSPWVNLSVQYSTFNDHLYFADSTTEDQIKLHQQIVAPAQYANTINYLSVK
                     ASNEFKFWKFALDNTILYQKVDQSDLILNVPEFLTRNTLYFSQEMFNKSLFFQTGVTF
                     NYFTNYYGNEYNPVIGEFFVQNKKEIGNYTNFDFFFNARIQRTRIYVTLEHFNSSLSG
                     YNYLASPTNPIGDFIIRLGLVWSFFQ"
     gene            2522..4099
                     /locus_tag="PLQ70_09060"
     CDS             2522..4099
                     /locus_tag="PLQ70_09060"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HQE34801.1"
                     /translation="MKKIQILIFIFSIIGSTANAQNWIPDLGNDTYKNPILYADYSDP
                     DIIRVGEDFYMVASSFNAMPGIPILHSKDLVNWTIINHIYTSLPLQKYEKPAHGEGSW
                     APSIRYHNGVFYVYFCTPNDGLFMASTKNPATKWSLNLVEKVERWEDPCPLWDDDGTA
                     YLIRGKVCANELYLHKMSSDGMQLLDNGKLIYKDILKQPIIEGPKFLKKDGFYYIFAP
                     AGSVPEGWQTALRSKNIYGPYEDKIILHQGNTIVNGPHQGGLVELESGEWWFMHFQSK
                     KPFGRIVHLQPVSWSKGWPIFGIDANNDGIGEPVSVYKKPNVGKNYPITIPQTSDEFN
                     DTIIGKQWQWHANPKESWFSLSENKGSLRLFSEKNFTQNGNLWFVPNLFLQKISSPSF
                     TAKTKLTFKGDLAGDRSGLVIMGAEWAYIALQKTTQGNKMVVYKGEKDKCEDLTQELE
                     SFSLNSDTCYLKVEFFDTNDFQFSYSLDDKQYVKISRKFKAQEGVWIGAKVGLFSLNP
                     NIQKSNGYSDFDWFRME"
     gene            4314..5150
                     /locus_tag="PLQ70_09065"
     CDS             4314..5150
                     /locus_tag="PLQ70_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQE34802.1"
                     /translation="MQYSKNILDTARQTPLIKLNKVVAEISGAVFAKVENFNLVNFAK
                     DRMILKMVEEAESNGNLKPGGTIIDGTLQDIGLGLALAANVKGYQLICIISDTQPKEK
                     TDILRAIGAKVIVCKTDAEATHPDSCFSISKKLSEEITDSWSVNPMDYLSNRTHYEQS
                     DIEIWEQTKGKITHLVFDINAVEISSGVGQFLKEKNPNIKILGIEIESLKRNATQENE
                     PYSLDGLIQVSEEDALLCSKELTLKEGIVAGKSSGAVLNGVLQLQQQFKPDDVIVVVF
                     ND"
     gene            5197..5679
                     /locus_tag="PLQ70_09070"
     CDS             5197..5679
                     /locus_tag="PLQ70_09070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQE34803.1"
                     /translation="MTFTAIDFETATSFHPCSVGIVTVENGVIVDEFVTLIKPPRNEY
                     SPFTIAVHGIYPRDTVNAKTFAQIFPEIQKRLQNRIVVAHNESFDRNVLAKSMALYGL
                     DYESLNIGSRWECTVKIYKSKGLKPTKLSDCCREMRIELNHHEALSDARACAKLYLLR
                     "
BASE COUNT         1968 a          873 c         1012 g         1914 t
ORIGIN      
        1 ttcaagatgt tgatttcatc aatttctttc ggatgcaaat gggttacggc aaatgtaatg
       61 gcttgggctt caataatagg ctttagtttt tctcgaactt tttcagataa ttgtttggaa
      121 tcgtttagga tggcgttttc aaaatcggtt ggaaggatta ctgccgcagc agttactgga
      181 ccagccagac aaccccgacc ggcttcatct gtgccagatt ctagaataaa attagaaaaa
      241 ttagagagca acatagtaga tggtattgga acacaaatat tataaaaatt tcttcaaaaa
      301 atgtttctat tttcaatttg ctaaaatatt cttataaatc aacacctttt tttatttatg
      361 tgatttttca tttacctttg ccttgattaa aaaaatcgtt tttcataatt ataccctttc
      421 tcgatgagaa ttttctttct ggttgttttg ttgttttgct cgacgttgct tttttctcaa
      481 gagaaaaata gtagaaaacc caatattttg gataatctga ttggcaaaga ttcagtaaaa
      541 aagaatgagc cagttgctac aatagacatg tatcgagtga ttactttgga acgagacacg
      601 acttacatcg atacttcgct tacaattcaa aagaaataca gtcataatta tttgcgaaaa
      661 gacctttttg gacttttggc ttttcctaac gaggggcaga cttataatac tttgcaatac
      721 agtttgaata cgttttcgcc acttccggaa tttggtttca aagccaagca ttttaatttt
      781 attgaagcca atcaaatacg gtattgttca gtagcgactc ctgttaccga attttatttt
      841 aaatcagtga tgcaaaaagg gcaaaatgta gatgcttttt ttacggtaaa tacttctcca
      901 agatttaatt tttcgttagc ctacaaagga ttgcgctctg atgggaaata catcaatcaa
      961 atggcaagta ctggaaattt taggtttaca gcgagttata ataccaaaaa caagcgctat
     1021 tttgcaaaca cacattttac tggacaagac attttgaatg aagaaaatgg aggaattacc
     1081 gatgtcgaag cttttgaaag tggtaatccc gattatttaa cacgagaacg attagaagtc
     1141 tatttgaaag atgcacggtc ttttctaaaa ggaaaacgag tgtttttgga tcattttttt
     1201 agaatcaatt cggtcaaagg gaataataat ttgtatgttt ctcatcaatt caattacgaa
     1261 aataaatttt ttgaatacaa tcagcccaca gttacttcta ctgttggcgg aacaactgta
     1321 tatcgatttg gtgactctta caaaaccagt ggtataaacg atcaaaccca ttataataag
     1381 acctataaca aagtgggatt ggcttatgaa aattccactt tagggaaatt ccattttttt
     1441 gtagatgatt tttttagcaa ttattattac aatcaaatat taattttgga cgatcaaacg
     1501 attccagctt ctttaagtcg aagaattgat agttatggag gacaatatga ttaccagaaa
     1561 aataaatgga ctggaaagtt tttgttttct aggtcattta ccaatcaatc tctttctaat
     1621 ttggatgcta agatgcaata tgaattgaac gacgaaacct tgctttcttt tcagtaccaa
     1681 aacataaata agttgcctaa tgacaactat aatttgtatc aaagtagctt tgtaaattac
     1741 aattggtcta atgattttaa gaatgagaaa atcaattcga tcattgtcaa tgctaattca
     1801 ccttgggtga atctttcagt acaatattca acattcaatg atcatttgta ttttgccgat
     1861 agtacaactg aggatcaaat taaattgcat caacaaattg ttgcacctgc tcaatacgca
     1921 aatacaatta actatttatc ggtgaaagcc agtaatgaat tcaagttttg gaaatttgct
     1981 ttagacaata ctattttata tcaaaaagta gatcagtcag atttaatact taatgtccca
     2041 gaatttctaa ccagaaacac tttgtacttt tctcaagaga tgtttaataa atccttgttt
     2101 tttcaaacag gggtgacatt taattatttt acaaactatt acggaaatga atataatcct
     2161 gtaattgggg agttttttgt tcaaaacaag aaagaaattg ggaattacac caacttcgat
     2221 tttttcttca atgctagaat tcagcgcact agaatctatg tgactttaga acattttaat
     2281 tcttcgcttt caggatacaa ttatttggct tcaccgacta atccaatagg cgattttatc
     2341 attcgattag gattggtttg gagttttttc caataaggtt gaaaataatt ttttataatt
     2401 cgatttataa gttgaaattc aaacatcaaa ccaaatgaga atttgagatt tgtactagtt
     2461 tggaacaatg aaaaatagaa tccttgagaa aaaaatagtg tttttaaaat ttgacataag
     2521 tatgaaaaaa atacaaatac tcatttttat tttctcaatt attggtagta ctgctaatgc
     2581 acaaaattgg attcctgatt tgggtaacga cacttataaa aatccaatac tttatgcaga
     2641 ttattccgat ccagatataa ttcgagtagg agaggatttc tacatggtgg cttcaagctt
     2701 taatgcaatg cctggaattc ctattttgca ctctaaagat ttagtcaact ggacgataat
     2761 aaatcatatt tatactagtt tgcctcttca aaaatatgaa aaaccagcac acggcgaagg
     2821 ctcttgggct ccttcaattc ggtatcacaa cggtgttttt tatgtgtatt tctgtacccc
     2881 aaacgatggt ctctttatgg catcaactaa aaacccagct acaaaatgga gtctaaacct
     2941 tgtagaaaaa gttgaaagat gggaagatcc ttgtccgtta tgggatgacg atggtactgc
     3001 ttatttgatt agaggaaaag tttgtgcaaa tgaattatac cttcataaaa tgagtagtga
     3061 tggcatgcaa ctactcgata atgggaaatt aatttataaa gacattctca aacaacccat
     3121 catcgaaggt cccaaatttt taaaaaaaga cggtttctat tatatttttg ctccagcagg
     3181 aagtgttcct gaaggatggc aaacagcttt gcgctcaaaa aatatatatg gcccctatga
     3241 agataaaata attttgcatc aaggtaatac aattgtaaat gggccacatc aaggaggtct
     3301 tgttgaatta gaatcaggag agtggtggtt tatgcatttt caaagtaaga aacctttcgg
     3361 aagaattgtt catttacaac ctgtttcttg gtcaaaaggt tggcctattt ttggaattga
     3421 tgctaataat gatggaattg gtgagcctgt tagtgtgtac aaaaaaccca atgttggcaa
     3481 aaactatccc ataactattc cgcaaaccag tgatgaattt aatgatacga ttatcgggaa
     3541 acaatggcaa tggcacgcca atcctaaaga gagttggttt tcattatctg aaaataaagg
     3601 gagtcttagg ttgttttctg aaaagaattt tactcaaaat ggaaacctat ggtttgttcc
     3661 taatttattt cttcaaaaaa tttcttcccc ttcatttact gcaaaaacaa aacttacatt
     3721 caaaggagat ttggcaggtg atagaagcgg attagtgata atgggggcag aatgggcata
     3781 tattgcgctt cagaaaacga ctcaaggcaa taaaatggtt gtctataaag gtgaaaaaga
     3841 taaatgcgaa gatcttactc aagaactaga atcattttct ttaaattcag atacttgtta
     3901 tttgaaagtg gagttttttg ataccaacga ttttcaattt agttatagtc tagacgacaa
     3961 acaatatgtt aagattagca ggaaatttaa ggctcaagaa ggagtatgga ttggagccaa
     4021 agtcggtttg ttttctctaa atcccaatat tcaaaaaagt aatggttact ccgattttga
     4081 ttggtttaga atggaatgat tcgtctttaa aatgtggttg tgtgtaatta atcaaacgga
     4141 ttctcattat atcttttgta ctgtggcata actgaattcg cgttttttga aaattctaat
     4201 taagggttat caagaagaat tttattttgt atttttacca aaacactaaa aaaactaagt
     4261 ttgctgcttt aaggtatttt cttttgtttt ttcaaattat agtaaagtta taaatgcaat
     4321 attcgaaaaa catattagat accgcaaggc aaacaccatt gataaaactc aataaagtgg
     4381 ttgctgaaat ttctggggca gtgtttgcca aagtagaaaa cttcaatcta gtcaattttg
     4441 ctaaagacag aatgatttta aaaatggttg aagaagcgga gagcaatgga aatttaaaac
     4501 ccggaggaac tattattgac ggtactttgc aagatattgg tcttggactt gcattagccg
     4561 ccaatgtcaa aggataccag ttgatttgca ttatttcaga tactcagcct aaagaaaaaa
     4621 cagatattct tcgagctatt ggagcaaaag taatcgtttg taaaactgac gctgaagcaa
     4681 ctcatcctga ttcttgtttt tctatttcta aaaaattatc tgaagaaatt acagatagtt
     4741 ggtctgtaaa cccaatggat tatctgtcga atcgaactca ttatgagcaa tcagatatcg
     4801 aaatttggga acaaactaaa ggcaaaatca ctcatttggt ttttgatata aatgcagttg
     4861 aaattagttc aggagttggc caatttttaa aagaaaagaa tccaaatatt aaaattttgg
     4921 gaattgaaat cgaaagtttg aaaaggaatg ctactcaaga aaatgaacct tattcactag
     4981 atggtttaat acaagtttcg gaagaagatg ctctactttg ctctaaagaa ttaacactca
     5041 aagaaggaat agtagcagga aaatcatctg gtgcagtatt aaatggagtg ctgcaactcc
     5101 aacaacaatt caagcctgat gatgtaattg ttgttgtatt caatgattaa aatttaattt
     5161 agaaaaaaag aacaaccaaa agaaatacaa tcttaaatga cttttaccgc aatagatttt
     5221 gaaacagcca cttcctttca tccttgttca gtagggattg ttactgtcga aaatggggtt
     5281 attgttgacg aattcgttac tttaattaag ccaccaagaa atgaatattc gccttttacc
     5341 atagcagttc acggtattta tccgcgtgat actgtaaatg ctaaaacttt tgcacaaata
     5401 tttcccgaaa ttcaaaaacg attgcaaaac cgcattgttg tggctcataa cgaaagcttt
     5461 gatagaaatg ttttggccaa atcgatggcg ctttacggct tagattatga atccttgaat
     5521 ataggttcgc gttgggaatg cactgtaaaa atttataaat ctaaaggttt aaaaccaaca
     5581 aaattaagtg attgttgtcg tgaaatgaga attgaactca accatcacga agctttatct
     5641 gatgctcggg cttgtgccaa attgtattta cttcgctagt tttttttaaa atgtaaactt
     5701 attccttttt ttattacttt agtatttaat ctaatagtct aaaaatgaaa gaagattttc
     5761 tccatta
//
LOCUS       DAOSNN010000175         5704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_14293_length_5704_cov_4.650735, whole genome shotgun sequence.
ACCESSION   DAOSNN010000175 DAOSNN010000000
VERSION     DAOSNN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14293_length_5704_cov_4.650735"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..1018
                     /gene="pdhA"
                     /locus_tag="PLQ70_09075"
     CDS             20..1018
                     /gene="pdhA"
                     /locus_tag="PLQ70_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HQE34804.1"
                     /translation="MKEVTKEVYLKWYEDMLLWRKFEDKLAALYIQQKIRGFLHLYNG
                     QEAVLAGALHAMDLTKDKMITAYRNHVQPIGMGVDPRRVMAELMGKATGTSKGMGGSM
                     HIFSKEHGFYGGHGIVGGQIPLGAGIAFGDKYNNTGGVTLTYFGDGASRQGSLHEAFN
                     MAMLWNLPVVFIVENNGYAMGTSVERTANHPDIWKLGLGYEMPCGPVDGMNPVKVAEA
                     MSEAIERARNNGGPTFLEMKTYRYRGHSMSDAQLYRSKEEVEEYKKIDPITQVLDVIK
                     DQKYATDAEIEVIDQRVKDLVEECVKFSEESPFPEIQQLYDVVYAQENYPFTPHKL"
     gene            1026..2651
                     /locus_tag="PLQ70_09080"
     CDS             1026..2651
                     /locus_tag="PLQ70_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HQE34805.1"
                     /translation="MATIITMPRLSDTMTEGTVATWLKKVGDKVSEGDMLAEIETDKA
                     TMEFESFNEGTLLYIGIKEGETAPIDSLLAIIGNEGEDISALISGAAAPKAEAPVEAK
                     KEEAAAPAAATTAALPAGVVVVTMPRLSDTMTEGTVATWLKKVGDKVAEGDMLAEIET
                     DKATMEFESFNEGTLLYIGIKEGESAPIDSLLAIIGPAGTDVSGIIASGGTAPVAAPS
                     EEAKAAPAAAKAEPVVQEAAANGQRILASPLAKKIASEKGIQLNQVKGSGENGRITKS
                     DVENFTPQATAAPAASTSAAPAKSEAAAPVAKPYVPAGQVATEEIKNSTMRKIIAKRL
                     AESLFTAPHYNLVIEVTMDEAMKSRAVINSVPDTKVSFNDMVIKASALALKKHPKINS
                     QWSADAITINYHVNIGVAVAVEDGLVVPVLPFTDGMSLSQIGASVRDLAGRAKNKKLL
                     PTEMEGSTFTISNLGMFGITEFNSIINQPNSAILSVGAIVEKPVVKNGQIVVGNTMML
                     SLACDHRTIDGATGAQFLQTLKQYIENPVTMLA"
     gene            complement(2743..3264)
                     /locus_tag="PLQ70_09085"
     CDS             complement(2743..3264)
                     /locus_tag="PLQ70_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34806.1"
                     /translation="MNINLKIMKMKFYLILICFFALTSCNLREINYSDDEIKLMKRNI
                     VNHNDTISYADYDLYISNNSDFDSIYQNELLAYSLKIKEVYPNAYYDIFSNFLRINND
                     GKFNPNDILKLAKPEQDLLVYYLEEGAKKEQYYCIDVLAKFYEEGIYFEKNLKKSDSL
                     TNINIYPKEVSRP"
     gene            complement(3275..4516)
                     /locus_tag="PLQ70_09090"
     CDS             complement(3275..4516)
                     /locus_tag="PLQ70_09090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE34807.1"
                     /translation="MNANQHVLIIGFVWPEPNSSAAGGRMVQLISIFKQQGFEVTFTS
                     PAMDSDFMVDLESLGVVKKSIALNCSSFDVFVKKLNPTIVLFDRFMIEEQFGWRVAEN
                     CPNALRLLDTEDLHCLRLARQKAFKEKREFSTNDLLTEEVAKREIASILRCDVSLMIS
                     EYEIELLQTVFKIDSNLLYYLPLLLEPIEDSTIQNLPTFEERNDFVFIGNFLHEPNWN
                     AVQYLKETIWPLIRKQMPEAVLNVYGAYPSQKVVQLNNVKEGFLVKGRAENAQEVVKN
                     ARIVLAPLRFGAGIKGKLLEAMQCGTPSVTTTIGAESMSGDLPWNGFIADDVQVFVAK
                     AVELYQDKTLWLKAQENGFEIMEKRYLKSLFIDDFVKHILEVQIHLKQHRFHNFMGTL
                     LQHHTLTSTKYMSRWIEEKNK"
     gene            4621..5202
                     /locus_tag="PLQ70_09095"
     CDS             4621..5202
                     /locus_tag="PLQ70_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HQE34808.1"
                     /translation="MLGLKLVTDPKWVNIVESNIEEILTDHAWCEQKAATNIITIITY
                     NSEHEELVTDLLEIAKEELEHFQMVHNIIKERGLKLGRERKDHYVNELFKFMKKDGSR
                     NDAFVDRLLFSAMIEARSCERFKVLSQNIEDKELAEFYRKLMISEAGHYTTFLAFARK
                     YSEKVDVDKRWAEWLDFEGKLITNYGKSETVHG"
     gene            5256..>5704
                     /locus_tag="PLQ70_09100"
     CDS             5256..>5704
                     /locus_tag="PLQ70_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HQE34809.1"
                     /translation="MDATKVKSILVKTAKYIGISLAVVLALMFVTPLIFSDKIKEEIK
                     KTANERLAGEMNYSDVNVSFFKHFPSLTLTLNDFKLNGSAPFQKEHLINSKEVSFGIN
                     VYSLVFGKSIKIDEIYLSNAAINIKVNKEGLANYNVYIAEKDEEVNK"
BASE COUNT         2016 a         1034 c         1045 g         1609 t
ORIGIN      
        1 agcaaaataa acaattcaaa tgaaagaagt tacaaaagaa gtttatttaa agtggtacga
       61 agacatgcta ctttggagaa agtttgaaga caaacttgca gcattgtaca ttcaacaaaa
      121 aattagagga tttctacatt tatataatgg tcaagaagcc gtattagctg gtgccttaca
      181 cgctatggac ttaacaaaag acaaaatgat tactgcatac agaaaccacg ttcagccaat
      241 cggaatgggt gttgacccaa gaagagtaat ggcagaattg atgggaaaag ctacaggaac
      301 ttccaaagga atgggtggct ctatgcacat tttctctaaa gaacacggtt tttatggtgg
      361 acacggaatc gttggagggc aaatacctct aggagctgga attgcatttg gagacaaata
      421 caacaataca ggcggagtta cattgactta ctttggtgat ggtgcctccc gtcaaggttc
      481 acttcacgaa gccttcaata tggcaatgct ttggaatctt ccagtggtat ttatcgttga
      541 aaacaacggt tatgctatgg ggacttctgt agaaagaact gcaaaccatc cagatatttg
      601 gaaattgggt ttaggttacg aaatgccgtg tggaccagtt gatggaatga acccagtgaa
      661 agttgcagaa gctatgagcg aagccattga aagagcaaga aacaacggag gaccaacttt
      721 ccttgaaatg aaaacatacc gttacagagg ccactctatg tctgacgcac aattgtaccg
      781 ttcgaaagag gaagtggaag aatacaaaaa aattgacccg attactcaag ttttggatgt
      841 aatcaaagat caaaaatatg ctaccgatgc cgaaatcgag gttatcgacc aaagagtgaa
      901 agatttagtt gaagaatgcg tgaaattctc cgaagaatct cctttcccag aaattcaaca
      961 attatacgac gtagtttacg ctcaagaaaa ctacccattc acacctcata aattataaat
     1021 caattatggc aacaataatt acaatgcccc gtttgagcga tacaatgaca gaaggaacgg
     1081 tagcaacttg gttaaaaaaa gtaggagaca aagtaagcga aggcgatatg ctagctgaaa
     1141 ttgaaaccga caaagcaaca atggaatttg aatctttcaa cgaaggaact cttttataca
     1201 ttggaattaa agaaggagaa accgctccaa ttgactcttt attagctatt atcggaaacg
     1261 aaggcgaaga tatttctgct ttaattagcg gagcagcagc tccaaaagcc gaagctccag
     1321 ttgaagctaa aaaagaagaa gcagctgctc ctgcagcagc aactacagca gctttaccag
     1381 caggagttgt cgtagtgaca atgcctcgtt tgagcgacac aatgaccgaa ggaacagtag
     1441 ctacttggtt gaaaaaagta ggcgacaaag ttgccgaagg cgatatgctt gccgaaatcg
     1501 aaaccgacaa agcaacaatg gaatttgaat ccttcaacga aggaacttta ttatacatcg
     1561 gaattaaaga aggtgaatcc gctccaatcg atagtttatt ggcaattata ggaccagcag
     1621 gaacagatgt tagtggaatt attgctagtg gaggaactgc accagtagcc gctccatctg
     1681 aagaagcaaa agctgctcca gcagccgcaa aagcagaacc tgttgttcaa gaagcagctg
     1741 ctaacggaca aagaattttg gcttcgccat tggcaaagaa aattgcaagt gaaaaaggaa
     1801 ttcaactaaa tcaagtaaaa ggttcaggag aaaatgggcg tatcactaaa agtgatgtgg
     1861 agaactttac gcctcaagct actgctgctc cagccgcttc cacaagtgct gctccagcta
     1921 aatcagaagc agctgcacct gttgcaaaac cttatgttcc agcaggacaa gttgccaccg
     1981 aagagattaa aaactctaca atgcgtaaaa tcattgcgaa acgtttggca gaatctttgt
     2041 tcactgcacc acattacaac ttggtaatcg aagtaacaat ggacgaggcg atgaaatcaa
     2101 gagcagtcat taacagcgtt cctgatacca aagtatcttt taacgatatg gtgattaaag
     2161 cttctgcatt ggctttgaaa aaacatccaa aaatcaattc acaatggtct gctgatgcaa
     2221 tcactattaa ctatcacgtg aatattggtg ttgccgttgc ggttgaagac ggattggttg
     2281 ttccagtatt gccgtttact gacggtatga gtttatctca aattggagcc agcgtaagag
     2341 accttgcagg aagagccaaa aacaaaaaat tattgccaac cgaaatggaa ggaagcactt
     2401 tcactatttc caaccttgga atgtttggaa ttactgaatt caactccatc atcaaccaac
     2461 cgaattctgc catcctatcc gtaggagcga ttgtagaaaa accggtggtg aaaaatggac
     2521 aaatcgtggt aggaaacact atgatgttat ctttggcttg tgatcacaga actatcgacg
     2581 gtgcaaccgg agcacagttc ttgcaaactt tgaaacaata tattgaaaac ccagtgacga
     2641 tgctagctta atagcttttc actttaataa tagaaatccc gttttgagta aatcaaaacg
     2701 ggattttttt ctttctacaa tacattgaaa ctgatacata cctcaaggcc tacttacttc
     2761 tttggggtaa atattaatat tcgtcaaact atctgatttt tttaaatttt tctcgaagta
     2821 gattccttct tcataaaatt ttgctaaaac atcaatacaa taatactgtt ctttttttgc
     2881 tccctcttct aaataataga caagtaaatc ttgctcaggt ttagctagtt ttagtatgtc
     2941 attaggatta aactttccat cattatttat tcttaaaaaa ttcgaaaaaa tatcataata
     3001 agcattagga taaacttctt taatttttaa agaataagca agcaactcat tttggtaaat
     3061 tgagtcaaaa tcgctattgt tacttatata caaatcataa tcagcataac taattgtgtc
     3121 attatgattc acaatgttcc gtttcatcaa tttaatttca tcgtcagaat aattaatttc
     3181 tctcaaatta caagaggtta aagcaaaaaa acatattagt attaaataaa atttcatctt
     3241 cataattttt aaattaatat tcatttttca aaattcactt attcttctcc tcaatccatc
     3301 gcgacatata tttcgtacta gttaaagtat gatgctgcaa tagtgttccc ataaaattat
     3361 ggaatcggtg ttgttttaaa tggatttgta cttctaaaat atgcttgaca aaatcatcaa
     3421 taaacaagga ttttaaataa cgtttttcca taatttcaaa accgttttct tgggctttta
     3481 gccaaagggt tttgtcttga tacaattcca ccgctttagc gacaaaaacc tgaacatcat
     3541 cagctataaa tccattccaa ggcaaatcgc cagacattga ttcggctcca atagtggttg
     3601 ttacgcttgg cgttccgcat tgcattgctt cgagtaattt tccttttatg cctgcgccaa
     3661 aacgtaacgg agctaaaact attcgagcat ttttcaccac ttcttgagca ttttcggcac
     3721 gtccttttac taaaaaacct tccttgacat tattcaattg cactactttt tgcgaaggat
     3781 aagcaccata aacattcaaa acagcttccg gcatttgctt tctgatgagt ggccaaatcg
     3841 tttctttcag gtattgaact gcattccaat tgggttcgtg aagaaagttt ccaatgaaaa
     3901 caaagtcatt tcgttcttcg aaagttggca aattttggat agttgaatct tcaataggtt
     3961 ccaacaacaa aggcaaataa tacaataaat tcgagtcgat tttgaaaaca gtttgcaaca
     4021 attcgatttc atattcggaa atcatcaagg aaacatcgca acgcaaaata ctggcaattt
     4081 ctcttttagc aacttcttcg gtcaataaat cgtttgtaga gaattctctt ttttccttaa
     4141 atgctttttg acgggctaga cgtaaacaat gcaaatcttc ggtatccaac aatcgcaaag
     4201 cattgggaca attttcggca actcgccagc caaattgctc ttcgatcata aaacgatcaa
     4261 acaagacaat agttggattt aatttcttta caaaaacatc aaaactggaa caattcaaag
     4321 caatggattt cttgacaacg cctaatgact ccaaatcaac cataaaatca ctgtccatcg
     4381 caggactggt aaaagtaact tcaaaacctt gttgtttaaa aatggaaatc aactgcacca
     4441 tccttcctcc tgctgccgaa gaattgggct ctggccaaac aaaaccaata attaaaacgt
     4501 gctgatttgc attcataaat tccattttaa gacacaaaat aagggaaata atccgaaaat
     4561 caatctgtta tagcataaat aaaaactgta tttttgtaaa taattttcat aagcaaaaaa
     4621 atgttaggat taaaattagt taccgaccca aaatgggtaa acatcgtaga atcgaacatc
     4681 gaagaaatct tgaccgatca cgcttggtgc gaacaaaaag ctgcaacaaa catcatcact
     4741 ataattactt acaattcgga acacgaagaa ttagttacgg atttattgga aattgccaaa
     4801 gaagaattgg aacattttca aatggtacat aacatcatca aagaacgtgg actaaaattg
     4861 ggaagagaaa gaaaagatca ttatgtgaac gaacttttca aatttatgaa aaaagatggc
     4921 agccgtaacg atgcttttgt ggatagattg cttttctcgg caatgattga agccagaagc
     4981 tgcgagcgtt tcaaggtatt atctcaaaat attgaagata aagaattggc cgaattttac
     5041 cgaaaattaa tgattagcga agcaggacat tacaccactt ttctagcatt cgccagaaaa
     5101 tattcagaga aagttgacgt tgataaacgc tgggcagaat ggttagattt tgaaggcaaa
     5161 ctaattacaa actacggaaa aagcgaaacc gttcacggat aaaatctcat tatataaaga
     5221 cactgtaaat agttataaca tcagaaaatt aaaatatgga tgctacaaaa gtaaaatcaa
     5281 ttttggttaa aacggcaaaa tatatcggga taagtcttgc ggtagttttg gctctgatgt
     5341 ttgtcactcc tttaattttt tcagacaaaa taaaagaaga aatcaaaaaa acggctaatg
     5401 aaagactagc tggagaaatg aattattcgg atgttaatgt ttcatttttc aaacattttc
     5461 cgtcattgac cttaacttta aatgatttta aactcaatgg ttcggctcca tttcaaaaag
     5521 aacatttaat aaattcaaaa gaagtttctt ttgggattaa tgtgtacagt ttagtttttg
     5581 ggaaatccat aaaaattgat gaaatttatt tatctaatgc agcaataaac atcaaagtta
     5641 acaaagaagg attagccaat tacaacgttt acatcgccga aaaagatgag gaagtcaata
     5701 aaga
//
LOCUS       DAOSNN010000176         5688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_14344_length_5688_cov_5.399964, whole genome shotgun sequence.
ACCESSION   DAOSNN010000176 DAOSNN010000000
VERSION     DAOSNN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14344_length_5688_cov_5.399964"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLQ70_09105"
     CDS             complement(<1..742)
                     /locus_tag="PLQ70_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34810.1"
                     /translation="MLRTGLRNYINNFKGFRREVWILALITFINRAGTMVLPFLSKYL
                     KENLHFTYGQVGWIMVAFGLGSMLGSWLGGKLTDKIGFYKIMIFSLFTSGIAFFILQY
                     ITSFWGLCIGMFTIMAIADMFRPAMFVSLFVYAKPENRVRALSLVRLSVNLGFAAGPT
                     LGGLIILGMGYQGLFWVDGSTCILAILIFALKVKEKKRIPLAEGEILTVPNRKSIYND
                     KPFWLLLFTIFIIVMVFFQIFTTLPLYHH"
     gene            873..1475
                     /locus_tag="PLQ70_09110"
     CDS             873..1475
                     /locus_tag="PLQ70_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HQE34811.1"
                     /translation="MKTVLTLVFIALFNFGFGQEKFDTAAVEKFQKDLNTEYADAKTS
                     PLTAEDLKIFKTLDFYPISEKYFVTAKFIRTENEKPFEMKTSTSRRPLYVKYGEAHFT
                     IDGKPFQLNIYQNIELSQDSAYKDYLFLPFFDLTCGKESYIGGKYIDLKIPQGNTIAI
                     DFNTSYNPYCAYNHKYSCPKVPLENNLNIEIKAGVKKFHD"
     gene            1475..2131
                     /locus_tag="PLQ70_09115"
     CDS             1475..2131
                     /locus_tag="PLQ70_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQE34812.1"
                     /translation="MKYFNLHTHQYTNQPDVLELVNQYPQEFDVAIPFYSIGIHPLFI
                     DENRLESDFKVVDEKLALPECLALGECGLDKRSETPFEVQQLVFEKQLALAEKHQKPV
                     VIHCVAAFQELIEIKKRLKISVPMIVHGFSKKVELAKQLIDNGFYVSFGKNLLRYPEL
                     ESVFKSIPNDRFLLETDMIEEGIQEVYALAAKYKNLKLNELQEIVNGNYKAVFEFNKQ
                     "
     gene            2158..2871
                     /locus_tag="PLQ70_09120"
     CDS             2158..2871
                     /locus_tag="PLQ70_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HQE34813.1"
                     /translation="MKEWTERAELLFKAEGLEKLRNSTILVVGLGGVGSFAAEFLARA
                     GVGNMTIVDGDVVDITNINRQLPALHSTVGAPKVSIVGDRLMDINPELKLTRVEEFLS
                     PERAFDIVTTEFDYVLDCIDSVTPKLNLIIGAKRKKVKIISSMGAGGKMEASKVKVSD
                     ISKTINCKLARTIRKRLKKEKINKVKVVFSSEIQDEASLKMTDGSNFKKSFYGTNSYM
                     PGLFGLYAAETVIRYLLKK"
     gene            2913..3839
                     /locus_tag="PLQ70_09125"
     CDS             2913..3839
                     /locus_tag="PLQ70_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HQE34814.1"
                     /translation="MKYLNLIRYQNLIMLALMQFIFRYGFLELQNIPLALAHWQYILL
                     VLATVCIAAGGYIINNIMDVETDAENKPETLIVGKLISETKAYNLYFGFTVIGVVIGF
                     YLSNVIEKPSFASIFIIIAAMLYFYATNLKQTLLIGNFVVALLLLLSVIIIGIFDLYP
                     VTFEENRPVMGLLFGILLDYALFAFIINFIREIVKDLQDVNGDSIQEMSTLPIVFGVN
                     RTTKLVFVLSFIPIICIAYYINTNLFASGLIYATLYGLVFILAPLLYFSIKIVSAKET
                     QDFHHLSTVLKWILFFGILSIAVISLNIKCNA"
     gene            3832..4416
                     /locus_tag="PLQ70_09130"
     CDS             3832..4416
                     /locus_tag="PLQ70_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HQE34815.1"
                     /translation="MLKNKLQKYKLILASGSPRRQQFFKDLDLDFEIRLKEIEEIFPP
                     ELKAEEITNYLAELKASAFEGELQSNEILITSDTIVWHKNKALGKPKDAQGAFEILKS
                     LSNATHEVITSVCFKTNTTIEVFYEVTQVTFNELSDEAIQYYIENYKPFDKAGAYGIQ
                     EWIGFVGVSKIEGSYANVMGMPTDKVYEYLSKLA"
     gene            4427..4957
                     /locus_tag="PLQ70_09135"
     CDS             4427..4957
                     /locus_tag="PLQ70_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQE34816.1"
                     /translation="MIFLNNFTREAISTGILLVLVVLSRIIFSKLIRSFAKRSQTIEQ
                     RTRLVIKYIHLFVNIMAAFGLIIIWGVNTKDIIIAISSFTTVVGVAMIAQWSILSNIT
                     SGIILFFYFPFKIGDVIHIHDKDFPIEAEIEDIRAFYVYLRDKDGRIIVYPNNLLLQK
                     GISILKTPVAEKEFFD"
     gene            5050..5547
                     /locus_tag="PLQ70_09140"
     CDS             5050..5547
                     /locus_tag="PLQ70_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34817.1"
                     /translation="MKTLKLIIAGMFLIVSGAVQSQVSVSINIGNPPSWGPVGYSNVE
                     YYFLPDVQAYYDIRATQFIYLSNGKWCRSRYLPGPYRNYDLYGGYKVVLNDYHGSKPY
                     VHYNNHKVKYHKGYKGKPQKTIGNNPHFHSDNGNHYGNDNKGKDNKGNGNKGNGNNGH
                     GNGKH"
BASE COUNT         1985 a          890 c         1014 g         1799 t
ORIGIN      
        1 cgtgatgata cagcggaagg gtagtaaata tttgaaaaaa caccattaca ataataaaaa
       61 tggtgaataa gagcaaccaa aaaggtttgt cattataaat cgatttacga ttgggaacag
      121 tcagaatttc tccttcggct agtggaattc tttttttctc tttgaccttt aatgcaaaga
      181 ttaaaattgc aagaatacaa gtactaccat caacccaaaa taagccttga tagcccattc
      241 ctaagataat taatccgcct aaagtaggac cagcggcaaa accaagattt acagacaatc
      301 gtactaatga taaggcacga actctatttt cgggtttggc ataaacaaat aaggagacaa
      361 acattgcagg acgaaacata tctgcaatgg ccataatggt aaacattccg atacataacc
      421 cccaaaaact agtgatgtat tgtagaataa aaaaagcaat accactggta aaaaggctaa
      481 aaatcattat tttataaaac ccaattttat cagtcaattt gccacccaac caagagccca
      541 acatagaacc caagccaaaa gccaccataa tccatccgac ttgaccgtaa gtaaaatgca
      601 aattttcttt taaatattta gacaaaaaag gcaaaaccat agtacccgct ctattgataa
      661 aagtaatgag cgccaaaatc caaacttcac gtctgaaacc tttaaaattg ttgatataat
      721 tgcggagacc tgttctaagc ataagtaaat ttctagtttg caaagctaca aagattttgt
      781 ctttagtcat tgtgaggcac gaagctatct caccaaatta ttaacgttgc gactttctaa
      841 cttttaaact atttttgtcc aaaataataa taatgaaaac agttctcact ttagttttta
      901 tagcactatt caattttggt tttggccaag aaaagttcga cactgctgct gtcgaaaaat
      961 ttcaaaaaga tttaaatacc gaatatgccg atgctaaaac aagtccatta acagccgagg
     1021 atttgaaaat tttcaagact ttggattttt atcccatcag cgaaaaatat tttgtaacgg
     1081 caaaattcat ccgtaccgaa aacgaaaaac ctttcgagat gaaaacctct actagtagaa
     1141 ggcctttgta tgtaaaatat ggagaagctc attttacaat agatggaaaa ccatttcaat
     1201 tgaatattta ccaaaacatt gaattatccc aagattcagc atataaagat tatttattct
     1261 tgcctttttt cgatttgact tgcggaaaag aaagctacat tggcggaaaa tatatcgatt
     1321 tgaaaatacc tcaaggaaac acaatcgcga ttgatttcaa cacgtcttat aatccgtatt
     1381 gcgcttacaa tcataaatat tcttgcccaa aagtgccatt agaaaataat ttaaatattg
     1441 aaataaaagc gggagttaaa aaatttcacg attaatgaaa tactttaatt tacatacgca
     1501 tcaatacacc aatcagcctg atgttttgga attggttaat caatatccac aggaatttga
     1561 tgtagcaatt ccgttctatt ccattgggat tcatccttta tttattgacg aaaaccgttt
     1621 ggaatctgat tttaaagtgg tggacgaaaa attggctttg cccgaatgtt tggctctagg
     1681 cgaatgtggt ttagacaaac gcagcgaaac cccttttgaa gtgcaacaat tggtttttga
     1741 aaaacaatta gcattggccg aaaaacacca aaaacctgtt gtaattcatt gcgttgccgc
     1801 ttttcaggaa ctcatcgaaa tcaaaaaaag attgaaaatt agcgttccaa tgattgttca
     1861 cggattttcg aaaaaagtgg aactcgccaa acaattaatc gacaatggat tttatgtttc
     1921 gtttgggaaa aatttattgc gttacccgga attagaatcg gtttttaaaa gcattccaaa
     1981 tgacagattc ttgctagaaa ccgatatgat cgaggaagga attcaagagg tttatgcttt
     2041 ggcagcgaaa tataaaaatt tgaaattaaa cgaattacag gaaattgtaa atgggaatta
     2101 taaagcagtt tttgaattca acaaacaata aaccaaaaac aacaaacgat aaagataatg
     2161 aaagagtgga ccgagagagc agagctttta tttaaagcag aaggattaga aaaattaaga
     2221 aactccacta ttttagtggt gggattaggt ggagttggat cttttgctgc tgaatttttg
     2281 gcaagggctg gagttggaaa tatgactatc gttgacggtg atgtcgtaga tattacaaac
     2341 attaatagac aattgcctgc tttgcattct accgttggag caccaaaagt aagcatcgtt
     2401 ggagacagat taatggatat taatccagaa ttgaaattga caagagtcga agaattcctt
     2461 tcgccagaaa gagcttttga tatcgtaaca actgaatttg actatgtttt agattgcatt
     2521 gacagtgtga ctcccaaact caacttgatt attggagcca aacgcaaaaa agtgaaaatc
     2581 atttcctcga tgggagccgg cggaaaaatg gaagcctcaa aggttaaggt ttccgatatt
     2641 tccaaaacaa tcaattgcaa attggcacga accataagaa agcgtttgaa gaaagaaaaa
     2701 atcaataaag taaaagtcgt tttttcgtct gaaattcagg atgaagcgag tttgaaaatg
     2761 accgatggct ctaatttcaa gaaatctttc tatggaacca acagttatat gccgggcttg
     2821 ttcggtttgt atgctgccga aacggtgatt cgatatttat tgaaaaaata gttgttcgaa
     2881 aaaaatctaa aatctaaatt ctgcaatctg aaatgaaata cctaaatcta ataagatacc
     2941 aaaacctgat aatgcttgcc ttgatgcagt ttatttttag atatggtttt ctggaattgc
     3001 aaaatattcc tttggctttg gcgcattggc agtatatttt gttggttttg gcaactgttt
     3061 gtatagccgc tggaggctac attatcaaca atattatgga tgtagaaacc gatgccgaaa
     3121 acaagcctga aactcttatt gtaggtaaac tgatttccga aactaaggcg tataatttat
     3181 actttggttt taccgtaatt ggcgtggtta ttggttttta tttatcgaat gtaattgaaa
     3241 aacctagttt tgcctctatt tttataatta ttgctgcaat gctttatttt tatgccacga
     3301 acttgaaaca aactttgcta ataggaaatt ttgttgtggc tttgctactt ttgttaagtg
     3361 tcatcataat tggaattttc gatttgtatc ctgtaacttt tgaggaaaac agacctgtta
     3421 tgggattgct ttttggcatt cttctcgact acgctctttt tgcttttatc attaatttca
     3481 tccgtgagat tgttaaagat ttacaagacg taaatggtga ttctattcaa gaaatgagca
     3541 ctttgccaat tgtttttgga gtaaatcgaa caacgaagtt ggtttttgta ctgagtttta
     3601 ttccaataat ttgtatcgct tattacatta ataccaactt gtttgcttcg ggattgattt
     3661 atgccacttt atacggattg gttttcatat tggcgccact tttatacttt tcgataaaaa
     3721 ttgtatcggc aaaagaaaca caagatttcc atcatttgag tacggtttta aaatggattc
     3781 tgtttttcgg aatactttct attgcggtca tcagtctaaa tataaaatgc aatgcttaaa
     3841 aataaattac aaaaatacaa actgatatta gcttcgggtt cgccaagacg ccaacaattt
     3901 ttcaaggatt tagacctaga ttttgaaatt agattgaaag agatcgagga gatttttcct
     3961 ccagaactaa aagcagaaga aattaccaat tatttggctg aattaaaagc tagcgctttc
     4021 gaaggtgaat tgcaatccaa cgaaattttg ataacaagcg acaccattgt ttggcacaaa
     4081 aataaagctt tgggcaaacc aaaagacgca caaggtgcct ttgaaattct taaatcttta
     4141 tcgaatgcta ctcacgaagt aatcacctcg gtttgtttta aaaccaatac aacaattgaa
     4201 gtattttatg aagttaccca agttactttc aatgaattga gtgatgaagc gattcagtat
     4261 tatatagaaa actacaaacc atttgacaaa gcgggagctt acggtatcca ggaatggatt
     4321 ggttttgtgg gtgtttccaa aattgaaggt tcctatgcta acgttatggg aatgcctacc
     4381 gacaaagttt acgaatattt gagtaaatta gcctaaaaag taacctatga tatttttaaa
     4441 taattttacc agagaagcaa ttagcacggg aattttactg gtgttggtag tattgtctcg
     4501 gattattttt tcaaaattaa tacgaagttt tgccaaacga agtcaaacaa tcgaacaacg
     4561 aacgagattg gttatcaaat acattcattt gtttgtcaat attatggcag cttttggttt
     4621 gataatcatt tggggagtca ataccaagga tattattatt gccatttctt cttttacaac
     4681 cgttgtgggt gtggcgatga ttgcccaatg gtcgattttg agcaatatca cttcgggtat
     4741 cattttgttt ttttattttc ctttcaaaat tggggatgta atccatattc acgataaaga
     4801 ttttcccatc gaagccgaaa ttgaagacat cagggctttt tatgtttatt tgagagataa
     4861 ggatggaaga ataattgtgt atcccaataa tttgttgcta caaaaaggca tttcgatatt
     4921 aaaaactcca gtagctgaaa aagagttttt tgattgaaca aatgaataag aaatatattt
     4981 cggcatagtt attgtctaat gtttggtata attttttaat taagaataat tataaaaaaa
     5041 aacaaaaaga tgaaaacgtt aaaattaatt attgcgggaa tgtttcttat tgtatccggt
     5101 gcggtacaat cgcaagtttc ggtaagtatc aatataggta atcctccatc ttggggacca
     5161 gttggatatt ctaatgtaga atattatttt ttgccagatg ttcaagccta ttatgatatt
     5221 cgagccacac agtttatcta tttaagtaat ggaaaatggt gtcgatcaag atatttacct
     5281 ggaccatata gaaattatga tttgtatggt ggttataaag ttgttttgaa tgattatcac
     5341 ggatcaaaac cttatgttca ttataacaat cacaaagtaa aataccataa aggatacaaa
     5401 ggaaaacctc aaaaaaccat tggaaataat cctcattttc atagcgataa tggcaatcat
     5461 tatggcaatg acaataaagg aaaagacaat aaaggcaacg gaaataaagg aaatggaaat
     5521 aatggacacg gaaatggaaa acattaattt ctaatttagg tttaaaagag agagagtcaa
     5581 atttgatccc ctctcttttt tgttatttat caaacgaata attactatat ttgatttcat
     5641 tgcaaattta atttttgttg gtacaaaatt aacaaaattt gcaacgta
//
LOCUS       DAOSNN010000177         5680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_14364_length_5680_cov_4.695822, whole genome shotgun sequence.
ACCESSION   DAOSNN010000177 DAOSNN010000000
VERSION     DAOSNN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14364_length_5680_cov_4.695822"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1409)
                     /gene="glgA"
                     /locus_tag="PLQ70_09145"
     CDS             complement(183..1409)
                     /gene="glgA"
                     /locus_tag="PLQ70_09145"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HQE34818.1"
                     /translation="MKIALFSNEFPPNIYGGAGVHIDFLSQELAKLGQVEVRCFGDQN
                     VDTNSMHVRGINSSLTKMVDDANPHIKMFHNMSRNVEMSQATPEADVIHCHTWYTHLA
                     GIMTRELLQAPLILTTHSLETHRPWKVEQLGNGYFLSRWIENSAYNTADGIIAVSQQM
                     KEDVVEAYGVNPEKVTVIHNGIDPEFYQPTFDEKLLLELGIDPNIPFVLFVGRITRQK
                     GISQLISAAKHFNKNCQIVLCAGAPDTKEIAEETEALIADLCKERDGVILISEMLPRE
                     KIKVLYSHARVFACPSLYEPFGIINLEAMACETPVVGSHVGGIPEIIVEGESGYLIPL
                     ESVSRTDFNPAHPDAFQKAFAAKINILLDDEALATQMGKAGRERVLKIFSWESIAKTT
                     YDYYQKVIDGFVKEKA"
     gene            complement(1567..2664)
                     /locus_tag="PLQ70_09150"
     CDS             complement(1567..2664)
                     /locus_tag="PLQ70_09150"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HQE34819.1"
                     /translation="MKKNIQFRILLAMISLFMLQACGQTSNTKNTATTMENTINKPEN
                     PYYSNTDTTKLNVSDAEWKKVLSPDLYAVSRNADTERAFTGKLWNSDAKGTYYCAACG
                     NKLFKSDQKFASSCGWPSFFEQDNKNSVVYKPDNSYGMRRIEALCGRCDGHLGHLFDD
                     GPAPTGKRYCMNSIALDFVPDGVLANKNNLETITLGGGCYWCVEAVYEKLNGVKSVVS
                     GFAGGKIANPTYEEVCSGTTGHAEVVQITFDPSITNIDEIFKVFFTVHDPTTLNRQGA
                     DVGTQYRSVIFYANPKQEKEAHTIIDQLNAEKVFSSKVVTTIEPLTQFYKAEEYHQNY
                     YQNNKNQPYCQMVIQPKIEKFEKIFKDRLKK"
     gene            2817..3344
                     /gene="idi"
                     /locus_tag="PLQ70_09155"
     CDS             2817..3344
                     /gene="idi"
                     /locus_tag="PLQ70_09155"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HQE34820.1"
                     /translation="MEEENVILVNQNDEQIGLMPKMEAHEKAVLHRAFSVFVLNEKNE
                     IMLQQRASHKYHSPLLWTNTCCSHQRDGETNIQAGSRRLFEEMGFETSLKELFHFIYK
                     APFDNGLTEHELDHVMIGYYNYSPKINPDEVENWKWMSIEAVAKDMQLHPEIYTVWFK
                     IIFDEFYHYLEDHKI"
     gene            3386..3796
                     /locus_tag="PLQ70_09160"
     CDS             3386..3796
                     /locus_tag="PLQ70_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQE34821.1"
                     /translation="MKVTVSRKAHFNAAHRLYRKDWTFEQNDAVFGKCNNPSFHGHNY
                     ELIVSVTGEINPETGYVIDIKDLSDLILEEVEKPFDHKNLNLDVPEFENLNPTAENIV
                     VVIWNKIRKKIEPELDLEVVLFETPRNFVTYKGE"
     gene            3796..4251
                     /locus_tag="PLQ70_09165"
     CDS             3796..4251
                     /locus_tag="PLQ70_09165"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQE34822.1"
                     /translation="MALQVGDKIPHFSVKDNVGNDFNSESIIGKKAVVIYFYPKDNTP
                     GCTAQACSFRDQYQDFKDLGAEVIGISSDSVTSHEKFIQKYKLPFILLSDLDKKIRNL
                     FGVKTNLFGLLPGRVTYVADQNGIIQMVFDSLSATKHIPKALEMIKKIV"
     gene            4302..5270
                     /locus_tag="PLQ70_09170"
     CDS             4302..5270
                     /locus_tag="PLQ70_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HQE34823.1"
                     /translation="MKMKFYPLQFEPILKERIWGGEKLKTILNKPISSKITGESWEIS
                     TVEGDVSVIANGDLAGKSLNDIIKSNPEEVLGTEVYARFGKQFPLLFKYLDAREDLSI
                     QVHPNDELAKKRHNSFGKTEMWYVMQADTDSKLIVGFKEDSNAAEYLENLKNKTLLSI
                     LDNVAVQVGDVFFLETGTVHAIGAGMVIAEIQQTSDITYRLYDFDRKDAAGNERELHV
                     DLALDAINYNKIDTYKKYNKELNQSNVMVDCPYFTTNFLPLDGEVAVNKSGNSFTVYM
                     CVEGDFELEFDGVKSKYIKGDTILIPAALKSFVLRGKGSLLEIYIS"
     gene            5328..5597
                     /locus_tag="PLQ70_09175"
     CDS             5328..5597
                     /locus_tag="PLQ70_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34824.1"
                     /translation="MANVKNLKKDINYVLGDIIEAVYLFEISTTGKPTDETNALIDEA
                     IAAFDALITKVNAKNVENKKAHFKQINVELEQTANQLIAKINELQ"
BASE COUNT         1865 a          917 c         1041 g         1857 t
ORIGIN      
        1 aatttaatta taatacctaa aacagcaaca attataaatg atacaattgc ggtaatgtat
       61 ataaatgaca gaaaattttc aattagtaaa atcatcttat ttgttttcaa tatacttggc
      121 ttgcaataac tatgcaactc tcaaatttat aatagattta aaaggttatc tttaaaatta
      181 ccttaagcct tctccttcac aaaaccatca atcacttttt ggtaataatc ataagtagtt
      241 ttggcgatag attcccaact aaaaattttc aaaacacgtt ctcttccggc ttttcccatt
      301 tgggtggcga gggcttcgtc gtctaataaa atattaattt tggcagcaaa ggctttctgg
      361 aaagcgtctg gatgggctgg attaaaatcg gttctagaaa cgctttcgag tggtatcaaa
      421 taacccgatt ctccttcgac aataatttct ggaattccac ccacatgact tcctacgact
      481 ggcgtttcgc aagccatagc ttcgagattg atgatgccaa aaggttcgta caatgaagga
      541 catgcaaaaa cacgagcgtg actgtaaagc acttttattt tttcgcgagg caacatttcc
      601 gagattaaaa tgacgccatc tctttctttg cacaaatcgg caatcagtgc ttcggtttct
      661 tcggcaattt ccttggtgtc gggagctcca gcacaaagta cgatttggca atttttatta
      721 aaatgtttgg ctgccgaaat caattgcgaa attccttttt gacgggtaat acgccctaca
      781 aataacacaa acggaatgtt cggatcaatt cctaattcta aaagcaattt ttcgtcaaaa
      841 gtaggttggt aaaattcagg gtcaattccg ttatgaatta cagttacttt ttcaggattg
      901 acaccataag cttcgactac atcttctttc atttgttggc tcacggcaat aattccatcg
      961 gcggtgttgt aagcactatt ttctatccaa cgtgacagaa agtagccgtt tccaagttgt
     1021 tctactttcc aagggcgatg cgtttccaag ctgtgagtgg ttaaaatcaa tggtgcttga
     1081 agcaattctc gagtcataat tccagctaga tgcgtgtacc aagtgtggca atgaatgaca
     1141 tcggcctcag gtgtggcttg cgacatttcg acatttcgac tcatattatg aaacattttg
     1201 atgtgcggat tggcatcatc gaccattttt gttaaggaag aattaatgcc gcgaacgtgc
     1261 atcgaattag tatctacatt ttggtcacca aaacagcgta cttctacctg acctaatttg
     1321 gctaattctt ggcttaaaaa atcgatgtga acacctgctc ctccgtagat gtttggtgga
     1381 aattcattag aaaaaagcgc tattttcatt gtgtgagaat tttatattgt gaaagtaaat
     1441 tgtaacttat ttcaaatata ataaatatcg cttatctatc actataacaa caattgaaat
     1501 gtcaataaat tttgagggtt gaaaccaaaa aaaaacgaaa agaaattttc ggttttcaag
     1561 tcaattttat tttttcaatc ggtctttgaa aattttctca aatttttcga ttttgggttg
     1621 aatcaccatt tgacaataag gttgattttt attgttttga taataatttt gatgatattc
     1681 ttcggctttg tagaattgag ttagcggttc tatggttgtt acaactttac tgctaaatac
     1741 tttttcagca tttaattgat cgataattgt gtgtgcttct ttttcttgtt ttggattagc
     1801 ataaaaaatt accgaacggt attgcgttcc cacatcggca ccttgacggt ttagcgttgt
     1861 cgggtcgtga acagtaaaaa acactttgaa aatttcgtca atattagtaa tgcttgggtc
     1921 aaaagtaatt tgcactactt cggcgtgtcc tgttgttcct gagcagactt cttcataagt
     1981 aggattggct atttttcctc ctgcaaatcc tgaaaccact gactttactc cgtttaattt
     2041 ctcgtaaacg gcttccacac accaataaca tccgccacca agggtgatgg tttcaagatt
     2101 atttttgttt gctaaaacgc catccggaac aaaatctaat gcaattgaat tcatacagta
     2161 acgtttgcca gtaggagcag gtccatcgtc aaaaagatga cctaaatgtc cgtcgcatcg
     2221 accacaaagt gcttcaattc ttctcattcc atacgaattg tctggtttgt acaccacgct
     2281 atttttgttg tcttgttcaa aaaaactagg ccaaccacag ctactagcaa atttttggtc
     2341 ggatttgaat aatttattgc cacaagcggc acaataatag gtgccttttg catcagaatt
     2401 ccatagtttt ccagtaaaag ctctttcggt atcagcgttt cttgaaaccg catataaatc
     2461 tggtgatagt acttttttcc attcggcatc tgagacattt aatttagttg tgtcggtatt
     2521 ggagtaataa gggttttcgg gtttgttgat tgtgttttcc atagttgtag ctgtgttttt
     2581 agtatttgaa gtttgtccgc aagcttggag cataaatagc gaaatcattg cgagtaagat
     2641 tcgaaattga atattttttt tcatttttag attttaaatt ttaaaataaa ccaaactatt
     2701 tagaatgttt aaaattaagc ttttcaattc ttaaacagtc gttaaacttt tcacaaactt
     2761 tttagaccac aaaaaccaat ttcttttttt gtatttttgg caagctaaaa gagattatgg
     2821 aagaagagaa tgtaattttg gtcaaccaaa atgatgagca aataggcttg atgcctaaaa
     2881 tggaagcaca cgaaaaggca gttttgcatc gtgctttttc tgtttttgta ttgaatgaaa
     2941 aaaacgagat aatgcttcaa caacgggcaa gtcataaata tcactcacct ttgttgtgga
     3001 cgaatacgtg ctgtagtcat caacgcgatg gtgaaaccaa tattcaggct ggaagtcgcc
     3061 gattgtttga ggaaatgggt tttgaaacca gtttgaaaga attgtttcat tttatttaca
     3121 aagcaccatt cgataacggt ttgaccgaac atgaactaga tcacgtaatg attggctatt
     3181 ataactattc acctaaaata aatcctgacg aagttgaaaa ttggaaatgg atgagcattg
     3241 aagctgtagc aaaagacatg caattacatc ccgaaattta taccgtttgg ttcaaaatta
     3301 ttttcgatga attttatcat tacttagaag atcataagat ttgagaattc agattgcaat
     3361 cccaacttta aacctttaaa ctcgaatgaa agtcaccgtt tccagaaaag ctcattttaa
     3421 cgcagcccat cgattgtatc gaaaagattg gactttcgaa caaaacgatg ctgtttttgg
     3481 caaatgcaat aatcctagtt ttcacggaca caattacgaa ttaatagtta gtgttactgg
     3541 tgaaattaat ccagaaacgg gttatgtgat tgatattaag gatttaagtg atttgatttt
     3601 ggaagaagtc gaaaagcctt ttgatcataa aaacctcaat ttagatgttc ctgaatttga
     3661 aaacctcaat ccaactgccg agaatattgt agttgtgatt tggaataaaa ttcgaaaaaa
     3721 aatagaacca gagttagatt tagaagtggt tttatttgag actccaagaa attttgtaac
     3781 ctataaaggc gaataatggc attacaagta ggagataaaa tccctcattt tagtgtaaaa
     3841 gataatgttg gcaacgattt taactctgag tcaattattg gaaagaaagc tgttgtgatt
     3901 tatttttatc caaaagacaa tacgccaggt tgcactgcac aagcttgtag ttttcgagat
     3961 caataccaag attttaaaga tttaggagcc gaagttattg gcattagtag tgatagtgtt
     4021 acttctcacg aaaaatttat ccaaaaatat aaattaccct ttattttgct ttcggatttg
     4081 gataaaaaaa tcaggaatct gtttggtgta aaaactaatt tgtttggttt attgcccgga
     4141 agagtgactt atgttgctga tcagaatggt ataattcaaa tggtttttga tagtttatcg
     4201 gctactaaac atattccgaa agctttagaa atgattaaaa aaatagtata ataaaactgc
     4261 aaatagttta ttttgcacta acttcaattt gattaaaaca tataaaaatg aaattctatc
     4321 cattacaatt tgaacctatc ctaaaagaaa gaatttgggg tggcgaaaag ttaaaaacaa
     4381 tattaaacaa gcccatttct tctaaaatta ctggcgaaag ctgggaaata tcaactgttg
     4441 aaggagatgt aagtgtaatt gccaatggcg atttggctgg gaaatcgctt aatgacatca
     4501 tcaaatccaa tcctgaagaa gttttaggta cggaagttta tgctagattt ggaaaacaat
     4561 ttccgttgct ttttaagtat ttagacgcgc gtgaagattt gtccatacaa gttcatccca
     4621 atgatgagtt ggctaaaaag cgtcataatt cttttggaaa aacagaaatg tggtatgtga
     4681 tgcaagccga cacagattcg aaattaattg taggtttcaa agaggattct aatgcggcag
     4741 agtatttaga aaatctaaaa aacaaaacac tacttagtat tttggataat gtggctgtgc
     4801 aagtgggcga tgttttcttt cttgaaacag gtactgttca tgccattgga gctgggatgg
     4861 taattgccga aattcagcaa acttctgaca ttacctatcg tttgtatgac tttgatagaa
     4921 aagatgcggc aggaaatgaa agagaattgc acgtcgattt agctttggat gctattaatt
     4981 ataataaaat tgacacctat aaaaaataca acaaagagct aaatcaatcg aatgttatgg
     5041 tcgattgtcc ttattttacc actaatttcc ttccattaga tggggaagtt gcggtaaaca
     5101 aatctggaaa ttcatttaca gtgtatatgt gtgttgaggg agatttcgaa ctggaatttg
     5161 atggagtaaa aagtaaatat ataaaagggg atacaatttt aattccagct gctttgaaaa
     5221 gttttgtcct aagaggaaaa ggttcacttt tagaaattta tatttcttag tccgtaatac
     5281 aaagattatg tgtacttttg caaacgcaaa ttaaaattaa aaataaaatg gcaaacgtta
     5341 agaatttaaa aaaggacatc aactacgttt taggagatat tattgaagca gtttacttat
     5401 ttgaaatttc tactacagga aaaccaacag acgaaactaa cgctttaatt gatgaagcaa
     5461 tcgctgcttt tgatgctttg attacaaaag taaatgctaa gaatgttgaa aacaaaaaag
     5521 cccatttcaa acaaataaat gttgaattgg aacaaactgc aaatcaacta attgctaaaa
     5581 taaacgaatt acagtaaaaa aaagtcaata aaaaggtctg tttttctttg gaaaattaaa
     5641 aatcagactt atatttgcac ccgtaatgag tcgccagcgt
//
LOCUS       DAOSNN010000178         5622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_14552_length_5622_cov_4.884857, whole genome shotgun sequence.
ACCESSION   DAOSNN010000178 DAOSNN010000000
VERSION     DAOSNN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14552_length_5622_cov_4.884857"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="PLQ70_09180"
     CDS             <1..1357
                     /locus_tag="PLQ70_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34825.1"
                     /translation="DKATYKPEEKATINLSSKSKEGIAKSASFSLSVTDMNGELEDKD
                     LGSNIYSHFLMESDIRGKVYNPAYYFDPTNSKRLEYLDNLLLTQGWRDFVWKTMPKVD
                     ENSSYIIEKGFIISGNVKQTFGSRPLVNNKLTLTLKNKKQMNFFSAMTDSVGRFKFEN
                     PMFFGKTTLFLNNRNEKGKFGGEIVLDPTEYSPFPVLFKNSAFDFSERTRSIAKNVLK
                     KYVAYGVDPENMLNEVEIIGQKKNKVVTFYGSPDYSYTADENTKNLPGIYDILAEVPG
                     LVVNDRTLSIPGAGKGTPLVLIDNYPAMDGELNLVYPHEVAKIDIIRSAEVAKYFIID
                     MDSDENIAGVISIITNGQRGNKPKKESVHAFSGEIEGFQIARIFYSPNPEKPNPDLDD
                     KLDVRNTIYWNPYVHPDKTGNAMVSYNNSSVETKVKVDLEGITATGIPVVKKAYYTIK
                     K"
     gene            1364..3784
                     /locus_tag="PLQ70_09185"
     CDS             1364..3784
                     /locus_tag="PLQ70_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34826.1"
                     /translation="MTYSNNKNYCFFYFLWIVLSISFLQAQEQKTIPEIEKIYLHTDK
                     SIYFIGEDLWYKAYDVRASNNLLFDNSNILYVELISPDSEIIARNKTNIEIGLGHGDF
                     QLTDSLGVKPGVYQLRAYTNWNRNFGEDFVFKKNIEIIDAFKSYSKTDEAQNTATEKL
                     IAPMGNPNNFRIDFFPEGGSLIENVASIVGFKGVNSNGNPIDVTGEIYNSDDELVTPF
                     SSPHDGMGKFQMIPMEGKNYYAKIKTPDGKLIRIELPKVTKEGYSLSFKTFKGKNIIS
                     ILTNEKTLLQNPNAALTVVCKARGVSYFESSPTLTQTTFSFELSKDKTLDGISQITLY
                     DSNSKPQSERLIYIEKDQDLDVQLLTDKVIYKPYEKAIVNVVSKSKTGVAKSASFSVS
                     VTDMNRADEKNSGTTICSYFLLESDIRGKVFNPGYYFDPSNLKRLEHLDDLLLTQGWR
                     DFLWKSTSKFSDLTTYKAEKGITISGRVKQLFGNKAKVNNRVGLVLLNKNHMNSFKTF
                     TDVSGSFKFENLMFSGKTKMVLNSRNENGKYGGELIANPEQSPMPVSAIKEPFIWDES
                     TRLLVYNVFRKFTAFGVKPENMLDEIQIKGQKKKYAVVSYGAPDYKYIIDKEEAKKLS
                     NSTDLIEEQCKGVIPNNFSMELNSYFDESPENSKPIILIDGYQAYEAGELLGIAPEDI
                     LKIEVVSGYVVDALYGRGSVISVFTNGNRNKAVSENSYSVKQEIDGFYTERVFYSPTP
                     EQAELDNKLAVRNTIYWNPFVHPDATGNANLNFFNTKVETKVKVELEGITATGIPVAK
                     KVYYTIKK"
     gene            3913..5265
                     /locus_tag="PLQ70_09190"
     CDS             3913..5265
                     /locus_tag="PLQ70_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HQE34827.1"
                     /translation="MDVEILARIQFAFTIAFHYIYPPLSIGIGLIMVIFEGLYLKTGN
                     KDYEILTRFWIKIFALTFGIGVATGIIMEFEFGTNWAVYSRYVGDIFGSALAAEGLFA
                     FGLESTFLGILLFGWNRVKPWVHFVSTLGVFLGSMFSAVWIVVANSWQQTPAGYHIVG
                     TGLHARAEVTDFWEMVFNPSSVDRIIHTWQGAILAGAFLVLSVHAYYIRKGRFVEISK
                     KAFQISLVVATLFSLTQLLSGHSTADGVAVNQPAKLATMEGHFQKNSPADLYLLGWVD
                     KEKQTVTGIGIPGGLSFLVHQDFQAPIKGLNDFLVEDRPSQINAIFQFYHIMVAIGML
                     LIGMTLYASFLWWRGKLFETKWLMWIFSFSVILPQIANQVGWFTAEMGRQPWVVYGLL
                     RTSKAFSQEVSANQIVFSLVLFTVVYSLLLVLFLYSVNKKIKHGPYEEAVAFSNTKNF
                     "
     gene            5275..>5622
                     /locus_tag="PLQ70_09195"
     CDS             5275..>5622
                     /locus_tag="PLQ70_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HQE34828.1"
                     /translation="METFLGIDYPTLWYLVIGLLFSGYAILEGFDFGAGAWHLFFRKD
                     LSRRIAINAIGPIWDANQVWLVIGGGALFAGFPVMYATMLSAMYIPFMLFLMFNVLRA
                     AAIKFRSAEEMPWW"
BASE COUNT         1862 a          911 c         1095 g         1754 t
ORIGIN      
        1 cgataaagca acctataaac cagaagaaaa agcgacgata aatttgagtt caaaatcaaa
       61 agaaggaatt gccaaatcag ccagtttttc attgagtgta accgatatga atggagaatt
      121 agaagacaaa gatttaggct ccaatattta ctctcatttc ttgatggaat cggatattag
      181 aggcaaggtt tataatcccg cttattattt tgaccccaca aattcaaagc gtttggaata
      241 tttagataat ttgcttttaa ctcaaggctg gcgagatttt gtatggaaaa caatgccaaa
      301 agtggatgaa aacagcagtt atattataga aaaaggattt ataatatcgg gaaatgtaaa
      361 acaaactttc ggttcaagac ctttggtcaa caataaatta acgctaactt taaagaataa
      421 aaaacaaatg aattttttta gcgcaatgac agatagtgtt ggtcgtttta aatttgaaaa
      481 ccctatgttt tttggaaaaa ccactctgtt tttaaacaat agaaatgaaa aaggcaagtt
      541 cggcggcgaa atagtattag atcctactga atattcaccc tttcctgttt tgtttaaaaa
      601 ttcagcattc gatttttcag aaagaacccg ttccattgct aaaaatgtgt tgaaaaaata
      661 tgtagcttat ggtgtagatc cagaaaacat gttgaatgaa gtggaaatta taggccaaaa
      721 gaaaaacaaa gtggttacct tttatggaag tcctgattat agttatacag ctgatgaaaa
      781 cacaaagaat ctacctggta tttatgatat tttagctgaa gttccaggcc ttgtggttaa
      841 tgacagaaca cttagtattc caggtgcagg taaagggact ccacttgtat taattgataa
      901 ttatccagct atggatggtg aactaaattt agtttatcct catgaagttg ccaaaattga
      961 cataataaga tccgcagaag tggcaaaata ttttataata gatatggatt cagatgaaaa
     1021 cattgcaggt gtaatttcaa ttattacaaa tggacaaaga ggcaacaaac ccaaaaaaga
     1081 aagtgtgcat gcctttagtg gagaaataga aggttttcaa attgcccgta ttttctattc
     1141 acccaatcca gaaaagccaa atcccgactt ggatgataaa ttagatgtaa gaaacactat
     1201 ttattggaac ccttatgtgc atccagataa aacaggaaat gctatggtaa gttataacaa
     1261 tagtagtgtt gaaactaaag taaaagttga cttagaaggg attactgcca ctggaattcc
     1321 tgtggttaaa aaagcatatt atacaattaa aaaataaatt tatatgactt acagcaacaa
     1381 taaaaattat tgttttttct actttctatg gattgtttta tcaattagtt ttttgcaggc
     1441 gcaagaacaa aaaacgattc ccgagataga gaaaatttac cttcataccg acaaatccat
     1501 ctattttata ggggaagatt tatggtacaa agcctatgat gttcgagctt ccaataattt
     1561 actttttgac aatagcaata ttttgtatgt agaactcatc tctcccgatt ctgaaataat
     1621 tgcccgaaac aaaaccaata ttgaaatagg tttgggacac ggcgattttc aattgaccga
     1681 ttcgcttggc gtaaaaccag gggtttacca attacgggct tacaccaatt ggaatagaaa
     1741 ttttggagaa gattttgtgt ttaaaaaaaa tatcgaaatt atagatgctt ttaaatcgta
     1801 ttcaaaaacc gatgaggcac aaaataccgc taccgaaaag ctaatcgctc caatgggcaa
     1861 cccaaacaac tttagaattg atttctttcc cgagggaggt tcgcttatag aaaatgtggc
     1921 tagtattgta ggctttaaag gggtgaatag caatggaaat ccgatagatg ttacgggcga
     1981 aatttataat tccgatgatg aattagtcac acctttttca agtccacacg atgggatggg
     2041 gaaatttcaa atgattccca tggaaggaaa aaattattac gcaaaaatca aaactcccga
     2101 tggcaaatta atacgaatag aacttccaaa agtgactaaa gaaggctatt cgttaagttt
     2161 taaaaccttc aaaggcaaaa atattatatc catacttacc aacgaaaaaa ccttacttca
     2221 aaatccgaac gctgcactaa ctgtggtgtg caaagcaaga ggtgtttctt attttgaaag
     2281 ctcgccaaca ctaacccaaa ccacattttc ttttgaactt tccaaagata aaactctgga
     2341 tggtataagc caaataacac tttacgacag caattcgaaa ccccaaagcg agcgtttaat
     2401 ttatattgaa aaagatcagg atttagacgt tcaacttttg acagacaaag tgatatacaa
     2461 gccctatgaa aaagctattg taaatgtggt ttcaaaatcc aaaacaggcg ttgccaaatc
     2521 ggccagtttt tcagtaagcg ttaccgatat gaatagagcg gatgagaaaa attctggcac
     2581 tactatttgt tcttatttct tgttggagtc tgacattcga ggcaaagtat ttaatccggg
     2641 atactatttt gatccttcta atttaaaacg actagaacat ttagatgatt tacttttgac
     2701 gcaaggctgg cgagattttc tatggaaatc aacctcaaaa ttcagtgatt tgacaaccta
     2761 taaagcagaa aaaggcatta caatttctgg tagagtaaag caactttttg gaaataaagc
     2821 aaaagtaaac aacagagttg gtctcgtttt attgaataaa aaccatatga attctttcaa
     2881 gacttttaca gatgtttcag gaagttttaa atttgaaaat ctaatgtttt ctggaaaaac
     2941 caaaatggtt ttaaattcta gaaatgagaa tgggaaatat ggaggcgaat taatagcaaa
     3001 tcctgaacaa tctcctatgc cggtttctgc tataaaagaa ccctttattt gggatgaatc
     3061 aactcgctta ttagtatata atgtatttag aaaatttacc gcttttgggg taaaacccga
     3121 aaatatgttg gacgaaatcc aaatcaaagg ccaaaagaaa aaatacgcag ttgtctccta
     3181 tggagcaccc gattacaagt acattattga caaagaagag gcaaaaaaac tgagcaacag
     3241 taccgacctt attgaagaac aatgtaaggg agtaatcccg aacaatttta gtatggaatt
     3301 aaattcttat tttgatgaat caccagagaa cagcaagcca attattttga tagatggtta
     3361 tcaagcttat gaagccggag agctcttggg aattgcaccc gaggatatcc tgaaaataga
     3421 agtggtaagc ggatatgttg tagatgcttt gtacggaagg ggttctgtga tttccgtttt
     3481 tacgaatgga aacagaaaca aggctgtaag cgaaaactct tattccgtca agcaagaaat
     3541 cgatggtttt tacacagagc gtgtttttta ttcacccact ccagaacaag ccgagttgga
     3601 taataaactg gctgtaagaa acaccatcta ctggaatccg ttcgtgcatc cagacgcaac
     3661 aggtaatgcc aatttaaatt ttttcaacac taaggtcgaa accaaggtaa aagttgagct
     3721 ggaaggaatt accgctacag gaattccggt ggcaaaaaaa gtgtattaca ccatcaaaaa
     3781 ataaagattt ttttatggta tatccataaa tagacacaca ttttttttta atttctaatt
     3841 aataagttaa ttaacgaatg ttgaatgtca ttcccagatt tgttgcctaa atttgggtta
     3901 aaatctacta tcatggatgt cgaaatatta gctcgaatac aatttgcatt taccattgct
     3961 ttccattaca tctatccacc attaagtatc ggaattgggc tgataatggt tatttttgaa
     4021 ggcctttatc ttaaaaccgg aaacaaggat tatgaaattc taactcgttt ttggattaaa
     4081 atattcgcct tgacttttgg aattggcgtg gctaccggaa ttataatgga gtttgaattt
     4141 ggaaccaatt gggcggtcta ttctcgttat gttggtgata ttttcggaag tgccttggct
     4201 gccgaaggat tatttgcctt tggtttagaa agcacttttc taggtattct actatttgga
     4261 tggaatcgag taaaaccttg ggttcatttt gtttctactt tgggcgtatt tttaggttct
     4321 atgttctcag ctgtttggat tgtcgtggcc aattcttggc agcaaactcc agctggttat
     4381 catatcgtcg ggacaggttt gcacgctcgt gccgaagtga ctgatttctg ggaaatggtt
     4441 ttcaatccat cgagtgtcga tcgaattatt catacttggc aaggagcgat tttagctgga
     4501 gctttcttag ttttgagtgt tcacgcttat tatattagaa aaggacgttt tgtcgaaata
     4561 tctaaaaaag ctttccaaat ttctttggtg gtggctaccc tattttcgct tactcaattg
     4621 ctttcaggtc acagtactgc cgatggtgtt gccgtaaatc aacccgcaaa attggcgact
     4681 atggaaggac atttccagaa aaattctcct gccgatttgt atctcttggg ttgggtcgat
     4741 aaagaaaaac aaaccgttac gggaattgga attccaggtg gattatcatt tttagtacat
     4801 caagattttc aagctcccat aaaaggtttg aatgattttc ttgttgaaga cagacccagt
     4861 caaatcaatg ctatttttca gttttatcac attatggttg ccattggaat gctgttaatt
     4921 ggaatgacac tttatgctag tttcctttgg tggcgaggaa aattattcga aaccaaatgg
     4981 ctaatgtgga ttttttcttt ctcggttatt ttgcctcaaa tagccaatca agtgggctgg
     5041 ttcactgccg aaatgggaag acaaccttgg gtagtttatg gattattacg aaccagtaaa
     5101 gcattttctc aggaagtaag tgctaatcaa attgtgtttt cattggtatt gtttaccgtg
     5161 gtttactcgc ttttgttggt cttgtttttg tattcggtca ataaaaaaat aaaacacggc
     5221 ccttatgaag aagcagtggc attttcaaat actaaaaact tttaatcaaa acttatggaa
     5281 acttttttag gaatagatta tcccacttta tggtatttgg tcatcggatt gttgttttca
     5341 ggttatgcca ttttagaagg ttttgatttt ggtgcaggag cttggcattt gtttttcaga
     5401 aaagatttga gccgaagaat cgccatcaac gccattggtc ctatttggga tgccaatcaa
     5461 gtttggttgg tcattggcgg tggcgcatta tttgcaggat tcccagtaat gtatgctacg
     5521 atgttatcgg caatgtacat tccgtttatg ttatttttga tgtttaatgt actcagagca
     5581 gcagccatca agttcagaag tgccgaagaa atgccttggt gg
//
LOCUS       DAOSNN010000179         5613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_14576_length_5613_cov_6.352645, whole genome shotgun sequence.
ACCESSION   DAOSNN010000179 DAOSNN010000000
VERSION     DAOSNN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14576_length_5613_cov_6.352645"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLQ70_09200"
     CDS             <1..342
                     /locus_tag="PLQ70_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HQE34829.1"
                     /translation="WTELDVWSYIEQEQIEIPSIYFSHKRKVFLRDGMIWSHSPFVFQ
                     EEDEPTEERVVRFRTVGDMSCTAAVDSYAGTISEVVGEIRQSTISERGARIDDKRSEA
                     AMEKRKQQGYF"
     gene            488..1732
                     /locus_tag="PLQ70_09205"
     CDS             488..1732
                     /locus_tag="PLQ70_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQE34830.1"
                     /translation="MEVLKIATAGSVDDGKSTLIGRLLYDTQSLTTDKLEAIEKSSKQ
                     KGYDYLDFSLATDGLVAEREQGITIDVAHIYFSTAKKSYIIADTPGHVEYTRNMVTGA
                     STSQVSIILIDARKGVIEQTYRHFFINNLLRVKDVIVAVNKMDLVDYSEEVYNKIVAD
                     FQALNAKSAYKEQNVSYIPLSALTGDNVVETLGKMPWYTGQTILQHLENLESKDVYDN
                     GQARFPVQTVIRPKTEEYHDFRGYAGKLYGNTIKVGDAVTVLPSLTESKVTKIHFFDQ
                     QFDEASVGSSITIELENDINVTRGDMIVKSNELPKVEKDITTTVCWMDSKKLVAGAKY
                     FVQHNTNRVLAKIDSVKNVIATDYSGVTSASQLSINEIGEVTIKLSKAIYFDAYTDNK
                     SNGAFILIDATTNTTAGVGFIK"
     gene            1808..3898
                     /locus_tag="PLQ70_09210"
     CDS             1808..3898
                     /locus_tag="PLQ70_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HQE34831.1"
                     /translation="MESFRTEIENPIVQKDIIDLERKIALFRDGKIDDERFRSLRLAR
                     GVYGQRQEGVQMIRIKLPFGKVTSEQLHRISKVSDEYSRGRLHITTRQDIQIHFVSLD
                     RTPELWAELEKDDITLREACGNTVRNITASETAGIDVDEPFDVSPYAHAMFQFLLRNP
                     VCQEMGRKFKMSFSSSDKDTALSYLHDLGFIPRIVNGERGFKIMLGGGLGSQPHHAEL
                     LSEFVPANQIIPTTEGILRVFDRHGERSKRLKARMKFLIKDLGVEAFLKLVEEEKKAL
                     SCKSFEIDTTAFDGPIPEPLLVAPKVEIDDLEAFEAWKKSNVIQQKQAGYVAIGIKVL
                     VGDFYTDKARLLADLIKNYGANELRFSLRQDILIRNIKEDNLVFFYQELKKLDFVALG
                     YNSTADITACPGTDTCNLGIASSTGIATELERVLAVEYPQYANNQEIVIKISGCMNAC
                     GQHNMAHIGFQGMSINSGKLVAPGLQVLLGGGNLGNGSGRFADKVIKIPSRRGPAALR
                     SILDDFEANANEKSFLDYYDAQGEKYFFDLLTPLAETSNLTDEDFIDWGNADNYVKAI
                     GVGECAGVVIDLVATLIFEAKEKIIFASEALNDKKWSDAIYLAYAGFVNGAKALLLSE
                     NQKTNHHAGIIDLFDTVFIETNKIELKSTFKDLVYQINQNEPSEEFAAKYIQEATDFF
                     EAIEAYRAKELANG"
     gene            3891..4673
                     /gene="cobA"
                     /locus_tag="PLQ70_09215"
     CDS             3891..4673
                     /gene="cobA"
                     /locus_tag="PLQ70_09215"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HQE34832.1"
                     /translation="MDNIVKPKVTLVGAGPGDPDLLTIKGAKALAKANVVLYDALANE
                     EILALAPQKAIKIFVGKIRGCHEYSQDKINQLIVDNALTYGNVVRLKGGDPFIFGRGS
                     EEIEYIQSFGIPTEVIPGISSSIAVPANQGISLTKRGVSESFWVITGTTSDHQLSKDV
                     ALAAKSTATVVILMGMSKLEQIVSLFQNESKGETPVAIIQNGTMSNEKVGFGTIDTIS
                     KVVQENNLSSPAIIVIGEVVKERNKLKGFYKEFISNKVCARE"
     gene            4673..5254
                     /locus_tag="PLQ70_09220"
     CDS             4673..5254
                     /locus_tag="PLQ70_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HQE34833.1"
                     /translation="MERNELYPIFLKLHQLNVLIVGGGNVGLEKLSFMLKSSPNANVE
                     VVAPKFLPELEALAQKHPSVKLTYRKFNRWMLRKRNMVIACTDDLKVNKRVYDLSRKR
                     FLICNIADTPPLCDYYLGGIVTKGNVKIAISTNGKSPTTAKRLREFFEEVIPEDINKM
                     VENLNEYRKTLKGNFEDKVQKMNEITQVLKGKQ"
     gene            5482..>5613
                     /locus_tag="PLQ70_09225"
     CDS             5482..>5613
                     /locus_tag="PLQ70_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydrofolate--homocysteine
                     methyltransferase"
                     /protein_id="HQE34834.1"
                     /translation="MPNIQEEIKKRILVLDGAMGTMLQRYNFSEEDFRGEQFKDFPHS
                     "
BASE COUNT         1956 a          888 c         1135 g         1634 t
ORIGIN      
        1 tggacagaat tagatgtttg gagttatatc gagcaagagc aaatcgaaat accatcgatt
       61 tatttttcgc acaagcgtaa agtgttcttg agagacggca tgatttggtc acattcccct
      121 tttgtttttc aagaagaaga tgaaccaaca gaagaaagag tagttcgttt tagaaccgtt
      181 ggagatatga gttgtacggc tgcagttgat tcttatgcag gaacaatttc agaagtggtg
      241 ggcgaaatta gacaatcgac tatttctgaa agaggtgcta gaattgacga caaacgttcg
      301 gaagcagcaa tggagaaaag aaaacaacaa ggatattttt aatagttgat ggttgatggt
      361 ttttggttga tagtttaggt atcttcaata atcatcttta aaatttaata attaatagtt
      421 gttgtttgat ggttgatagt taagactacc aacaaccatc aaccaacaac caacaaccaa
      481 aaaaagaatg gaagttttaa aaatagcaac agcaggaagt gtagatgacg gaaaaagtac
      541 tttaatcgga agattattgt atgatacgca atccttgact acagataaat tagaagcaat
      601 agaaaaaagc agcaagcaaa aaggatacga ttacctcgat ttttctttgg ctaccgatgg
      661 tttggtagct gaaagagaac aaggaatcac gattgatgta gcacacattt atttttcgac
      721 tgccaaaaaa agttacatca ttgccgatac tccaggtcac gtagaatata caagaaatat
      781 ggttacagga gcttcgactt cgcaagtgtc tatcattttg atcgatgctc gtaaaggagt
      841 gattgagcaa acctaccgtc acttttttat caataattta ttgagagtaa aagatgtgat
      901 tgttgctgta aacaaaatgg atttggttga ttattcagaa gaagtttata ataaaattgt
      961 agccgacttt caagcattga atgccaaaag tgcttacaaa gaacaaaacg tgagttacat
     1021 tccgttgagc gccttgactg gagataatgt ggtagaaaca ttaggaaaaa tgccttggta
     1081 cacaggacaa accattctgc agcatttaga aaatttagaa tcgaaagatg tgtatgataa
     1141 tggtcaagct cgttttcctg tgcaaacggt tattcgccca aaaacagaag aataccacga
     1201 ttttagagga tatgctggaa aattatatgg taatactatt aaagttggag atgctgtaac
     1261 agttcttcct tctttgacag aatcgaaagt aaccaaaatt cacttttttg atcaacaatt
     1321 tgacgaggca agcgtgggtt catcaattac aatcgaattg gaaaatgata ttaatgtaac
     1381 tcgtggtgat atgattgtaa aatcaaacga attacctaaa gtagaaaaag acattacaac
     1441 aactgtttgt tggatggaca gcaaaaaatt agtagctgga gccaaatatt ttgtacaaca
     1501 caacaccaac agggttttgg ccaaaattga tagcgtgaaa aatgtaatcg ctaccgatta
     1561 ttcaggggtt acatcagcaa gccaattgag tattaacgaa ataggagaag taaccatcaa
     1621 gttgagcaaa gccatttatt ttgacgctta taccgataac aaatcaaatg gagcctttat
     1681 tttgattgat gcaacaacta atacaacagc aggagtagga tttattaaat agttgattgt
     1741 tgatgatttt tagttgatag tagagcaact aaaaaccatc aactaacaac caacactaaa
     1801 aaaagatatg gaaagtttta gaacagaaat agaaaaccca atagttcaaa aagatatcat
     1861 cgatttagaa agaaaaattg ctttattccg tgatgggaaa attgatgatg agcgttttcg
     1921 tagccttcgt ttagcgcgtg gtgtttacgg tcaacgtcag gaaggcgttc aaatgattcg
     1981 tatcaaatta cctttcggga aagtaacgag cgagcaattg catcgaattt caaaagtttc
     2041 tgatgaatat tcaagaggac gtttgcacat tacaactcgt caagatatcc aaattcactt
     2101 tgtgagttta gacagaacac ctgaactttg ggctgaatta gaaaaagacg atattacact
     2161 tcgtgaagct tgcggaaaca ctgttagaaa tattactgcc agtgaaactg ctggaatcga
     2221 tgttgatgaa ccttttgatg tgtcgccata tgcacacgct atgtttcagt ttctgttgag
     2281 aaatccagtt tgtcaggaaa tgggacgtaa attcaaaatg tcattttctt cttctgataa
     2341 agatactgca ctgagttatt tgcacgattt agggtttatt cctagaattg taaatggcga
     2401 aagaggtttc aaaattatgt tgggtggagg attaggctca caaccacatc acgccgaatt
     2461 attatcagag tttgttcctg ccaatcaaat tattccaact accgaaggaa ttctcagagt
     2521 ttttgaccgt cacggcgaaa gatccaaacg tttgaaagct cgtatgaaat tcttaatcaa
     2581 agatttagga gtagaagcgt tcttgaaatt ggtcgaagaa gagaaaaaag ctttgtcgtg
     2641 taaatctttc gagattgata caaccgcatt tgatggccca attccagaac cattattagt
     2701 tgctccaaaa gtagaaattg acgatcttga agcgttcgaa gcttggaaaa aatcgaatgt
     2761 aattcagcaa aaacaagcag gatatgtagc tattggaatc aaagttcttg tgggcgattt
     2821 ttatacagac aaagccagat tactagccga tttgataaaa aattatggag ctaacgaatt
     2881 gcgtttttca ttaagacaag acattcttat tcgaaatata aaagaggata atttagtctt
     2941 tttttaccaa gaattgaaga aattagattt cgtagcttta ggatataatt ctactgccga
     3001 cattaccgct tgtcctggaa ccgatacttg taatcttgga attgccagca gtacaggaat
     3061 tgctacagaa ttagaaagag ttttggctgt cgaatatcct caatatgcta acaatcaaga
     3121 aattgttata aaaattagtg gttgtatgaa tgcttgtgga caacacaata tggctcatat
     3181 tggttttcaa ggaatgtcta tcaattcagg aaaattagtg gctccagggc ttcaagtttt
     3241 gttaggagga ggaaatttag gaaatggatc aggaagattt gccgataaag taatcaaaat
     3301 tcctagtcgt cgtgggccag ctgctttgcg ttctattttg gatgattttg aagcgaatgc
     3361 caacgaaaaa tcctttttgg actattatga tgctcaaggc gaaaaatatt tcttcgactt
     3421 attgacacct cttgctgaaa cttctaatct tactgacgaa gattttatcg attggggaaa
     3481 tgccgataat tatgtaaaag ctattggtgt gggcgaatgt gctggtgtag taattgattt
     3541 agtagctact ttgatttttg aagctaaaga aaaaataatt tttgcaagtg aagctttgaa
     3601 tgacaagaaa tggtcagatg ctatttattt agcttatgct ggatttgtaa atggagcgaa
     3661 agcattatta ttatccgaaa accaaaaaac aaatcaccac gctggaatca ttgatttatt
     3721 cgatactgtt tttatagaaa ccaataaaat tgaattgaaa tctactttca aagacttggt
     3781 ttatcaaatc aatcaaaacg aaccgtctga ggaatttgca gcaaaataca ttcaagaagc
     3841 gaccgatttt tttgaagcta tcgaagccta cagagctaaa gaattagcaa atggataata
     3901 tcgttaaacc caaagtaact ttagtaggag caggtccggg cgatcccgat ttgctcacta
     3961 tcaaaggtgc aaaagcgttg gctaaggcca atgtggtttt gtatgatgct ttggcaaacg
     4021 aagaaatatt ggcattagca ccccaaaaag ctataaaaat atttgttgga aaaataagag
     4081 gttgtcacga atattcgcaa gacaaaatca atcaattaat tgtggataat gcgctgactt
     4141 atggtaatgt ggtccgacta aaaggaggcg atccattcat ctttggtcgt ggaagcgaag
     4201 aaatagaata cattcaaagc tttggaattc caacagaggt aattcctgga atttcatcat
     4261 ccattgcggt tcctgctaat cagggaattt ctttgaccaa aagaggcgtt tccgaaagct
     4321 tttgggtcat tacaggaaca acttcagatc atcaattgtc aaaagatgta gctttagcag
     4381 caaaatctac ggcaactgtt gtaattttga tgggaatgag taaattggaa caaattgtta
     4441 gtttatttca aaacgaatcc aaaggagaaa ctccagtggc tattattcaa aacggaacaa
     4501 tgtccaacga aaaagtaggt tttggaacca ttgatactat ttcgaaagta gttcaagaaa
     4561 ataatttgag ctcgccagcc atcatcgtta ttggcgaagt ggtaaaagag cgcaataaat
     4621 taaaaggatt ttacaaggaa tttatttcca ataaagtttg cgcaagagaa taatggaaag
     4681 aaacgaatta tacccaattt ttttaaaatt gcatcaactc aatgttctta tcgttggcgg
     4741 tggaaatgtg ggtttagaga aattgtcttt tatgctcaaa tccagtccta atgccaatgt
     4801 tgaggtagtt gcgcctaagt ttttgccaga attagaggcc ttagcacaaa aacacccttc
     4861 agtaaaatta acatacagaa aattcaatcg ctggatgctt cgcaaaagga atatggttat
     4921 cgcttgcacc gatgatttga aagtaaacaa gcgcgtttat gatttgtctc gaaaaagatt
     4981 cctaatttgt aatatcgcag acacaccgcc attgtgcgat tattatttag gtggaattgt
     5041 aaccaaagga aatgtaaaaa tcgccatttc tactaatgga aaatcaccaa caactgccaa
     5101 aaggttacgt gaattttttg aggaagtgat tccagaagac atcaacaaaa tggttgaaaa
     5161 cctgaacgaa taccgcaaaa ctttaaaagg aaattttgaa gataaagttc agaaaatgaa
     5221 cgaaatcacc caagttttaa aaggcaaaca ataaacactt cttatgtttg cgattcatcg
     5281 tgctatgtct ttactttgca acgccataaa acaatcgttt tttttggagc taattcttgt
     5341 tattcgctaa atctttgcgg ctgaacacca gccacaaagg ataacgcttc tatcagggct
     5401 aaaagaaaaa tagttagttt ttgcaaattc taatcagcct cgcgcggtag cgtctttgcg
     5461 gtgaaaaaat aaaaataaat tatgccaaac attcaagaag aaatcaaaaa acgaattctc
     5521 gtgctcgacg gagcaatggg aacgatgttg caacgctata atttctctga agaagatttc
     5581 cgaggagaac aattcaagga ttttccacat tcg
//
LOCUS       DAOSNN010000180         5562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_14744_length_5562_cov_5.727619, whole genome shotgun sequence.
ACCESSION   DAOSNN010000180 DAOSNN010000000
VERSION     DAOSNN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14744_length_5562_cov_5.727619"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..2284)
                     /locus_tag="PLQ70_09230"
     CDS             complement(8..2284)
                     /locus_tag="PLQ70_09230"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HQE34835.1"
                     /translation="MRQSSKLLICSFFLFFFGKIAAQSDHILWYKQPAEFFEESLVLG
                     NGKIGGTVFGGVNSDKIYLNDITLWSGEPVNANMSPEAYKNLPAIREALKNENYKLAE
                     ELNKKIQGKNSESYSPLGTIEINNHHNGKATNYYRELDISKAISKVSYEVDGVKFTRE
                     YFVSASDKIMIIKLTSSKKGALNFDINSNSLLKFKTEVKDNLLSMNGNAPIHENIEYD
                     KLTGNNRSSLYQSVVEVPLRKTDIEKGTRFTTLIKIKNTDGTITSSSSSLGVKNATEA
                     LIYVSIATSFNGFDKNPATEGLNDKAIALDNLNKAFSKSFDKLKQTHIADYQKFYNRV
                     SLNLGKTEAPNLPTDERLLRYSEGKEDKNLEVLYFNFGRYLLISSSRTLGVPANLQGL
                     WNPYLNPPWSSNYTTNINLEENYWLAENTNLSEMHLPLLSFIKNLSVTGKISAKTFYG
                     VDEGWSASHNSDIWAMTNPVGQFGKEEPMWACWNMGGAWLSTHIWEHYVFTQDKNYLK
                     NEGYQIMRGAAQFCLKWLVEDKNGNLITSPSTSPENQYVTPDGVVGATIYGGTADLAM
                     IRECFDKTIKASKVLNMDVEFRTKLETTVSKLHPYQIGKKGNLQEWYFDWNDKDSKHR
                     HQSQLFGLFPGDHINLLKTPELANACRQTLEIKGDETTGWSKGWRINLWARLWDGNRS
                     YKMFRELLRYVDPDGKKTEKPRRGGGTYPNLFDAHPPFQIDGNFGGAAAVAEMLVQSN
                     ETEIRLLPAFPMLGKAVL"
     gene            complement(2297..4246)
                     /locus_tag="PLQ70_09235"
     CDS             complement(2297..4246)
                     /locus_tag="PLQ70_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQE34836.1"
                     /translation="MKKSVLFLFLIFSLLSNANVRMPLLFSDGMVLQRNKPIPVWGWA
                     EANEKVEVRFNKQTKTVTADQNGKWMLNLDSEKAGGPFELTISGKNKIVITNVLVGEV
                     WICSGQSNMEFQVYKTMNAKAEIEDSNYPMIRHFGVAQDLSGTPKEDLKAGKWTVCNK
                     ENLPDFTAVGYFFSKKIYAELKIPIGIINTSWGGTCVETWTSRAAFEKSEEFKSMIAE
                     IPSVNMDAIFETYKKSVLDNLQKVQGFEVTMANENQFKDSNFDDKNWPEIKVPSLWEN
                     QQIGNIDGIVWMRKTIVLSAEQVQKEAVLHLSKVDDEDETYINGIKIGTNNLWDAQRV
                     YKIPAGVLKEGKNVIAVRISDYTGGGGIYGDAVDLKIDFKDSNIPLEGLWKFNVVKVK
                     IEISPNSYPSLLFNAMVNPLIPYAFQGVLWYQGEANVTRAKEYKKSFPLMINDWRTQW
                     KQGNFPFYFVQLSTFDEYGGNSEKGSRWAELREAQTETLKLPNTGMAVTTDIGNAKDI
                     HPTNKQDVGKRLAVIALNNVYGKKMVYSGPIFKSQEVKGNQIVLSFDNIGTGLSTTDP
                     TGILKGFEIAGADKIFYPAKAIIKDNKVIVSCDKIQNSVAVHYGWADDDTEINLFNKE
                     KFPASPFRTDNWEMITANEKYSVSK"
     gene            complement(4284..5348)
                     /locus_tag="PLQ70_09240"
     CDS             complement(4284..5348)
                     /locus_tag="PLQ70_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HQE34837.1"
                     /translation="MSSSAVQLAAKIKLGWNIGNTLEATGGETAWGNPKVTKALIDLV
                     KANGFNAIRIPCSWNQNLANTTTAQIKADWLARVKEVVQYCVDNDMYVLVNIHWDGGW
                     LENNCTNAKKVENNAKQKAFWEQIATSLRDFDEHLLFASANEPNVDDATQMDVLNSYH
                     QTFIDAVRSTGGKNAYRTLVVQGPSTDIEKTNKLMTTLPTDKVANRLMAEVHFYPYQF
                     TLMTKDETWGKMFYYWGAGNHSTTDTAHNPTWGEEADIDKFFLLMKNQFVDKGIPVIL
                     GEFGAIKRTNLTGNDLTLHLKSRADFLKYVVKSSKLNGLIPFYWDAGNLGVNTMSLFD
                     RSNNTVYDAQALTGLLDGLK"
     gene            complement(5470..5519)
                     /locus_tag="PLQ70_09245"
                     /pseudo
     CDS             complement(5470..5519)
                     /locus_tag="PLQ70_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024405.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1585 a         1123 c          961 g         1893 t
ORIGIN      
        1 attcctttca cagaaccgct ttcccaagca tcgggaaagc tggaagcaac ctgatttcgg
       61 tttcattaga ctgaaccaac atttcggcaa cggcagccgc tccgccaaaa tttccatcaa
      121 tttgaaacgg tggatgtgca tcgaataaat tgggataggt tcctcctcct cgtcttggtt
      181 tttcggtttt ctttccatca ggatcaacat aacgaagcaa ttcacgaaac attttataag
      241 aacgattgcc gtcccaaagt cttgcccaaa gattgatgcg ccaaccttta gaccaacctg
      301 tagtttcgtc gccttttatt tccaaagttt gcctacaagc attcgccaat tcaggtgttt
      361 ttaataaatt aatatgatcg cctggaaaaa gtccgaacaa ctgcgattga tgacgatgtt
      421 tagaatcttt gtcattccaa tcgaaatacc attcttgtaa attgcctttt tttccaattt
      481 gatagggatg caattttgaa actgtggttt caagtttagt tctaaattca acatccatat
      541 tcaacacttt cgaagcttta atggtcttgt caaaacattc acgaatcatc gccaaatcag
      601 ccgttcctcc ataaattgtg gcaccaacaa ctccatcggg agtaacgtat tgattttcgg
      661 gagaagttga tggcgaagtg attaaattac catttttatc ttcgaccaac catttcaaac
      721 aaaattgagc agcgccacgc ataatttggt aaccctcgtt tttgaggtaa tttttgtctt
      781 gtgtaaacac ataatgttcc caaatatgag tgctcaacca agctccgccc atattccaac
      841 acgcccacat cggttcttct tttccaaatt gaccaacggg attggtcatc gcccaaatat
      901 cagaattatg cgaagccgac caaccttcat ccactccgta aaatgtcttg gctgaaattt
      961 ttccggtaac cgaaagattt ttgataaaac tcaaaagtgg caaatgcatt tccgaaagat
     1021 tggtgttttc ggccaaccaa taattctctt cgagattgat gttggttgtg taattagaac
     1081 tccaaggcgg attgagatac ggattccaaa gtccttgcaa attggctgga acgcctaaag
     1141 ttctggaact gctaataagc aaataacgcc cgaaattgaa atataaaact tccagattct
     1201 tgtcctcttt tccttcggaa tagcgcaaca aacgttcgtc ggttggcaaa ttcggagctt
     1261 cggtttttcc taaatttaaa ctaacccgat tgtagaactt ttgataatca gcaatgtgag
     1321 tttgttttaa tttatcgaat gatttagaaa aagctttatt caaattgtcc aaggcaatgg
     1381 ctttgtcatt caatccttcg gttgcgggat ttttatcgaa accattaaaa ctcgtggcaa
     1441 tggatacata aatcaaggct tcggtggcgt ttttcactcc taaactagaa ctggaacttg
     1501 tgatggttcc atccgtattt ttaattttta tcaaagtggt gaatcgagta cctttttcta
     1561 tatccgtttt tcttagtggt acttctacta ctgattgata caatgatgat cgattgtttc
     1621 cagttaactt atcatactca atattttcgt gaataggggc atttccattc atcgaaagca
     1681 aattgtcttt aacttctgtt ttgaatttta gtaaactatt tgaattgatg tcaaaattca
     1741 atgctccttt tttgctgctt gtcagtttga taatcataat tttatctgaa gcagaaacaa
     1801 agtattcacg agtgaatttc actccatcca cttcataact cactttagaa atggctttgg
     1861 aaatatccaa ttccctgtaa taattggtag cttttccgtt gtgatgattg tttatttcaa
     1921 tcgtgcccaa tggcgaataa gattctgaat ttttaccttg aatctttttg ttcaattctt
     1981 cggcgagttt gtagttttcg tttttcaatg cttcgcgaat agctggcaaa tttttataag
     2041 cttcaggact catattagca ttgacaggct ctcccgacca tagtgtgata tcattcaaat
     2101 aaattttatc tgaattgact cctccaaaaa cggttcctcc tattttacca tttcccaaca
     2161 ccaagctttc ctcaaaaaat tctgctggtt gtttgtacca caaaatatgg tcagattgag
     2221 cagctatttt tccgaaaaag aataaaaaaa aggaacaaat aagtagtttg ctggattgcc
     2281 tcataattgc taaaaattat ttgcttacac tatatttttc attggcggta atcatttccc
     2341 aattatcagt tctgaaaggt gaagcaggga atttttcttt attgaaaaga ttgatttccg
     2401 tatcatcatc tgcccaacca taatgaactg caacagaatt ttgaatttta tcgcaagata
     2461 caattacttt attatccttg ataatagctt tcgcaggata gaaaattttg tcagcaccag
     2521 caatttcaaa tcctttcaaa attccggttg ggtctgtggt tgacaatcca gtacctatgt
     2581 tgtcaaaact taaaacgatt tggtttcctt ttacttcttg agatttaaaa ataggtcccg
     2641 agtaaaccat ttttttacca taaacattat ttaaggcaat tacagccaat cgtttcccaa
     2701 catcttgttt attggtagga tgaatatctt tggcgttgcc aatatcagtc gttacagcca
     2761 ttcccgtgtt gggcaatttc aacgtttcag tttgtgcttc gcgaagttct gcccaacgac
     2821 ttcctttttc actattaccg ccatactcat caaaagtgga taattgtaca aaatagaaag
     2881 gaaaattacc ttgtttccat tgagttctcc aatcgtttat cattaaggga aatgattttt
     2941 tgtattcttt tgctctagtt acattggctt cgccttgata ccacaaaacg ccttgaaaag
     3001 cataaggaat caatggattg accattgcat tgaacaataa tgaaggataa ctattgggag
     3061 aaatttctat ttttactttc actacattaa acttccaaag tccttctagc ggaatattcg
     3121 aatctttgaa atcgattttc aaatcgactg catcgccata aattccgcca ccacctgtat
     3181 aatcggaaat tcgaaccgca atgacattct tgccttcttt caaaacgccc gcaggaattt
     3241 tataaactct ttgggcatcc caaagattgt tggttccaat tttgattcca tttatgtagg
     3301 tttcgtcttc gtcgtccact ttggacagat gcagaacggc ttctttttgg acttgttccg
     3361 ccgataaaac aatcgttttt cgcatccaaa cgatgccgtc aatattgcct atttgctggt
     3421 tttcccaaag cgatggcact tttatttccg gccagttttt atcgtcaaaa ttcgagtctt
     3481 taaactggtt ttcattggcc attgttacct caaaaccttg cactttttgg agattatcta
     3541 aaaccgattt tttgtaggtt tcaaaaatgg catccatatt tactgaagga atttctgcaa
     3601 tcatagattt gaattcttca cttttttcga atgctgcccg acttgtccaa gtttctacac
     3661 aagttccacc ccaagaagta ttgatgattc ctattggaat ttttaattcg gcgtatattt
     3721 ttttggaaaa aaagtaacct actgccgtaa aatcaggaag attttctttg ttgcaaactg
     3781 tccattttcc agctttcaaa tcttctttgg gagtaccgct caaatcttgg gcaaccccaa
     3841 aatgccgaat catcggataa ttggaatctt cgatttcggc tttggcattc atcgttttat
     3901 agacttgaaa ttccatattg gattgtccgc tgcaaatcca gacttcgccc accaaaacat
     3961 tggttattac tattttattt ttgcccgaaa tggtcaattc aaaagggcct cctgcttttt
     4021 cggaatctaa attcagcatc cattttccgt tttggtcggc agtgacggtt ttggtttgtt
     4081 tgttgaaacg gacttctact ttttcattgg cctctgccca accccaaacc ggaatgggct
     4141 tgttgcgttg caaaaccatt ccgtcagaga acaataatgg cattcgaaca ttagcgttag
     4201 ataataaact aaatatcaga aaaaggaata ggacactttt tttcatttta aaattattta
     4261 caaatttgaa tctgattaat aaactatttt aatccgtcca gtaaaccagt taaagcctgt
     4321 gcatcataaa cagtattgtt gcttctatca aataaagaca ttgtgtttac tcccaaattt
     4381 ccagcgtccc aataaaaagg aatcagacca ttaagcttgg aacttttaac tacatatttc
     4441 aagaaatcag ctcttgattt taaatgtaaa gtcaaatcat tacctgtcaa attggtgcgt
     4501 ttaatggctc caaattcgcc caaaatgact ggaattcctt tatctacaaa ttgatttttc
     4561 attaaaagga aaaacttatc tatgtcagct tcttctcccc aagtaggatt atgagctgta
     4621 tcggtagtcg agtgattgcc tgcaccccag taataaaaca ttttgcccca ggtttcatct
     4681 ttggtcatta atgtaaattg ataaggataa aaatggactt cagccatcaa tcgattggca
     4741 actttatccg ttggcaaagt agtcattagt ttattcgttt tttctatgtc agttgaaggc
     4801 ccttgaacta ctaatgttcg gtacgcattt ttacctcctg ttgaacgaac ggcatcaata
     4861 aaggtttggt gataagagtt caaaacatcc atttgtgttg catcgtcaac gttgggttca
     4921 ttggcactgg caaaaagcaa atgctcgtca aaatctctta aactagtggc gatttgttcc
     4981 caaaaggctt tttgtttggc attattttct acttttttgg cattggtaca attattttct
     5041 aaccatccgc cgtcccagtg aatgtttact aatacgtaca tatcattgtc aacacaatat
     5101 tgaacgactt cttttacacg agcaagccaa tcagctttga tttgagctgt tgtagtgttg
     5161 gccaaatttt gattccagga acaaggaatt cgaatggcgt tgaaaccatt ggctttgact
     5221 aaatcgatca aggctttggt cacttttgga tttccccaag ccgtttctcc gccagtggct
     5281 tcaagtgtgt tgccaatatt ccaacccaat ttaatttttg ctgccaattg aaccgctgaa
     5341 ctgctcattc ctgtggcatc tgcagcgatt ggattagtat tgtaacttgg atataaatca
     5401 gttaccggag gaacaactcc tggttgagtt attgcaatta agaccgaagg agcttgactt
     5461 gaactgacag taattgttgc tgaacgagtg gtagttccag tatttgcagt tgctgtaata
     5521 ttgaagaagt tgttcctaat gttcctgatg ttttacttat tt
//
LOCUS       DAOSNN010000181         5560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_14750_length_5560_cov_4.433061, whole genome shotgun sequence.
ACCESSION   DAOSNN010000181 DAOSNN010000000
VERSION     DAOSNN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14750_length_5560_cov_4.433061"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLQ70_09250"
     CDS             complement(<1..509)
                     /locus_tag="PLQ70_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HQE34838.1"
                     /translation="MKIVFATNNKNKILEVQQMLPQTIEIISLESIGCFEEIPETATT
                     IEENAIMKANYVTEKYGYDCFADDTGLEVEALNGEPGVFSARYAGEQRNSDDNMNKLL
                     KTLTGEPNRNAQFKTVIALNLKGKQQCFTGIAKGEITIEKTGNQGFGYDPIFKPEGFD
                     VTFAQLSLV"
     gene            714..1157
                     /locus_tag="PLQ70_09255"
     CDS             714..1157
                     /locus_tag="PLQ70_09255"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQE34839.1"
                     /translation="MKKQSILFLLTLIIGTVFTSCTKDISGPVDMNYLEGKWNFNKST
                     ASSSGFTIPYSTDYLKNEDGCNKDYVEFITGGIVRFGDYKPNCVFEEKIGTWSQNGNS
                     IIINVVGSNFNGTFDVASLSANELILKIDGTYGGKSGTLNLYFTK"
     gene            complement(1520..1846)
                     /locus_tag="PLQ70_09260"
     CDS             complement(1520..1846)
                     /locus_tag="PLQ70_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34840.1"
                     /translation="MATLDGKHSFGSIGETRVTFVEKGVDEIRKDFLKQLLEHNGFEV
                     VLEEEKKKAEEDPQLYTVAVTDMVFNPTIWIFERKMKTLDGHRVTQDYWFQRTTETNP
                     MYWKNS"
     gene            complement(1850..3256)
                     /locus_tag="PLQ70_09265"
     CDS             complement(1850..3256)
                     /locus_tag="PLQ70_09265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HQE34841.1"
                     /translation="MRQEIFLLTILLLLVVGEIFIHKNKKGTLTHLALFLFGIHTIIG
                     FFAIEEASLFGGMFRTNSLIHFFKNALNVGVLIVLLQSADWIQEKMVPLNKGNEFFML
                     LFSSLLGMYFMISAGDFLMFYIGLELSTLPVAALVAWETSKRISSEAGVKFILSAGLA
                     SGTSLFGISLLYAATGSIYFADITSVLTSTNFTILGFVLFFSGLAFKISLVPFHFWTA
                     DVYEGAPIGVASYLSVISKGAAAFILMILLFTVLKPLMHVWENIIYVVALATMFIGNL
                     FALRQQNMKRFLAFSSIAQAGFILLGLITGTQLGTATIVYFVLVYVFTNLAAFGVVQA
                     ISLQTGKENRDDYNGLYRTNPNLSLVMMLALFSLAGIPPVAGFFGKFFLFTAAASKGY
                     YLLVFLAVVNVTISLYYYLLVVRAMFIRRSDNPIPFFKNKIYMRLGLILTVLGILILG
                     LYSPLYDYIFELSTIFNK"
     gene            complement(3291..4787)
                     /locus_tag="PLQ70_09270"
     CDS             complement(3291..4787)
                     /locus_tag="PLQ70_09270"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQE34842.1"
                     /translation="MDILSLFVIVPVLTVLALVFSKGLKQARLISMIGSFIQLGMAIN
                     LLFAYFRERAVDKSVMLFTQDYVWFKNFNVHYAIGVDGISVALLLLTAIVVLAGVFIS
                     WKTDDLPKEFFVSLLVLATGVYGFFISIDLFTMFVFYEIAVIPMYLLIGIWGSGPKEY
                     SAMKLTLMLMGASAVLMVGVLGIYFNSNADGGALTFNILEIAKVHIPFEAQKLFFPLT
                     FVGFAVLGALFPFHTWSPDGHASAPTAVSMLHAGVLMKLGGYGVFRIAMFLLPLGAIE
                     WSWFFIILSATGVIYGAFGALKQTDLKYINAYSSVSHLGMVLFALLMLNKTAWNGAIL
                     QSLSHGFMTALFFALIGMIYGRTHTRDITKLGGLLKVMPFISVIYVIAGMASLGLPGF
                     SGFIAEMNIFVGAFQHEGMFYRVATIISVSAIVVTAVYILRVLGIMLMGEVKNDEYLN
                     LPKATWFEITGILLLLIPMLGMGVAPLWLSDMTMDSIQPFIQGVLNHL"
     gene            complement(4801..>5560)
                     /locus_tag="PLQ70_09275"
     CDS             complement(4801..>5560)
                     /locus_tag="PLQ70_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQE34843.1"
                     /translation="KDMGSLRKYMPITNITFLIAALAIAGVPPFAGFWSKDEILVAAF
                     EKNQLLYFVGLFVAGLTAFYMFRLYFGIFWGKETKYHHEPHESPMSMAFPLLFLAVMS
                     IVAGFIPFSEFITADKAGFEGHLDLPLAAVAVGTGLVGIILAWIFYKKENDLAERFSN
                     AFGMFYKWTFHKFYFDELYLFITRKIIFNLISAPIAKFDKKYVDGTMEGIGNKTVIIS
                     EKIKGLQSGRLQDYAMFFVSGVVVVALVFIYLWT"
BASE COUNT         1916 a         1019 c          926 g         1699 t
ORIGIN      
        1 tctacgagcg aaagttgtgc aaaagtgaca tcaaaacctt ctggtttaaa gataggatca
       61 tagccaaaac cttgatttcc tgttttttct attgtaattt ccccttttgc gattcctgtg
      121 aaacactgct gtttgccttt taggtttaag gcaattacgg ttttaaactg agcatttcta
      181 ttcggttctc ctgtcaaagt ctttaataat ttattcatat tatcgtccga attgcgttgt
      241 tctccggcat atcgagctga gaaaacgcca ggttcaccgt ttagcgcttc tacttctagt
      301 ccagtatcgt ctgcaaagca atcgtagccg tatttttcag tcacataatt ggctttcata
      361 attgcatttt cttcgatagt tgtagcagtt tctggaattt cttcgaaaca gccaatactt
      421 tcgaggctaa taatttcgat agtttgagga agcatttgtt gaacttcaag gattttattt
      481 ttgttattgg ttgcaaaaac gattttcata attcagtttt aattggataa agaacaaagg
      541 tatcttattt ttaaaatggt tttgtgaaac tcaattgtaa tttattatat caatatttat
      601 aaaaacctaa gttttaactt aatatttatc gttattgtag ttaatgaact gatttgtttt
      661 ttggttggat attttttaat tattattgta gcgctaaccc aatttgaaaa acaatgaaaa
      721 aacaaagtat tttattcctt ttgacattaa ttataggaac agtttttact tcttgcacta
      781 aagatattag tggtcctgta gatatgaatt atctagaagg aaaatggaat ttcaataaat
      841 caactgcttc ttcaagcggt tttactattc cttactcaac agattattta aaaaatgaag
      901 atggatgtaa taaagattat gtcgaattta ttactggagg tatagttaga tttggtgatt
      961 ataaacccaa ttgtgttttt gaagaaaaaa ttgggacttg gtctcaaaat ggaaatagta
     1021 ttataataaa tgttgtaggt tctaatttta atggcacttt tgatgtagca agtctttctg
     1081 caaacgagtt aatattaaaa attgatggaa cctatggtgg gaaatcagga acattaaatc
     1141 tctattttac gaaataactt gtggctgtat tattttttat acaaaagatg aatacaaact
     1201 taatttggtg ttaaaatatt gaaaaacact gtgattaatc aaaattaatc gtaaatttac
     1261 gtgtgattct gattaattac ttgagcatat aatatcataa atatttaata ttagaaaaag
     1321 tatctcaata gtcagctata tttaaggttt aagtgtaagt tagataatga taaatcagaa
     1381 tttacaaatg ggaggtctgt aaaaaggcct ccttttttgt gttagatatt cagaatcact
     1441 ttcaataaaa aaaccaatct attactagat tggctttctg ggttttggtt aatttaattt
     1501 tggttggtta atataaaatt tatgaattct tccaatacat tgggttggtt tctgtagttc
     1561 tttgaaacca ataatcctga gttactctgt gtccgtctag agttttcatt tttctttcga
     1621 aaatccagat ggtgggattg aaaaccatat cggtaactgc aactgtatat aattgaggat
     1681 cttcttccgc ttttttcttt tcttcttcta aaaccacttc aaagccattg tgctctaaga
     1741 gttgtttcaa gaaatctttt cgaatttcgt caacgccttt ttcaacaaaa gtgactcttg
     1801 tttctccaat gcttccaaat gaatgttttc cgtctaatgt tgccataact tatttattaa
     1861 aaatggtgct taattcaaaa atgtaatcgt acaatgggct gtataatcct agaataagaa
     1921 tacccaaaac ggtgagtatt aatcccaatc tcatataaat tttgttcttg aaaaatggaa
     1981 tcggattgtc acttcttctg atgaacatgg ctcttactac taataaatag taataaagcg
     2041 agatggttac attaactacc gccaagaaaa ccaataggta atagcctttg cttgcggctg
     2101 ctgtgaaaag gaaaaacttc ccaaaaaatc ctgccactgg tggaattcca gccaaagaga
     2161 acaaggccaa catcattacc aaactcaagt taggattggt tctgtacaaa ccgttgtaat
     2221 cgtctctgtt ttctttgcct gtttgcaagg aaatggcttg aaccacacca aaagcggcca
     2281 aattagtgaa tacataaact agtacaaaat aaacgattgt ggctgttcct aattgtgttc
     2341 ctgtgattaa acccaacaaa ataaatccag cttgtgcaat ggatgaaaag gccaagaatc
     2401 gtttcatgtt ttgttggcgc aaagcaaata aattaccgat aaacatcgtt gctaatgcta
     2461 ccacataaat aatgttttcc caaacgtgca tcaatggttt tagaacggta aataataaaa
     2521 tcatcaagat gaaagccgca gctccttttg aaattacgga taaataggaa gccacaccaa
     2581 tgggggcacc ttcatacaca tcggctgtcc aaaagtgaaa gggaactaaa gaaattttga
     2641 atgccaatcc agagaaaaac aatacaaaac ctaatattgt aaagttcgtt gaagttaaaa
     2701 cgcttgtaat gtcagcgaaa taaatcgatc ctgtggcagc atacaaaaga gagattccga
     2761 ataaggatgt tcccgatgcc aatcctgctg aaagaatgaa ttttacaccc gcttcactcg
     2821 aaattctttt ggaagtttcc caagctacca aagcggctac aggcaaagta gataattcca
     2881 atccgatgta aaacattaga aaatcacctg ccgaaatcat aaaatacatt cccaaaagag
     2941 aagaaaatag caacataaag aattcatttc ctttgtttaa cggcaccatt ttttcttgaa
     3001 tccaatcggc tgattgaagt aggacaatca aaacacctac attaagtgca tttttgaaaa
     3061 agtgaatgag tgaattcgtg cgaaacattc cgccaaataa actcgcttct tcaatggcaa
     3121 aaaagccgat aatagtgtga atcccaaata aaaataaagc aagatgcgta agtgtacctt
     3181 ttttgttttt atggatgaaa atttcgccta caaccaacaa taataaaatt gtcaagagga
     3241 aaatttcttg tctcattgcg ataaaactgt tcaaattcat tttcgttatt ttataaatga
     3301 tttaatactc cttgaataaa aggttggata ctatccattg tcatatcgct caaccaaagt
     3361 ggagcaacac ccataccaag cattggaatt aatagtagca aaatacctgt aatttcgaac
     3421 caagttgctt ttggtaaatt taaatattcg tcgtttttga cttctcccat tagcataatt
     3481 cctaatactc gaagaatgta aaccgccgtt accacaatgg ccgaaactga aattatggtc
     3541 gctactctgt aaaacattcc ttcgtgttgg aacgctccga caaagatgtt catttcagca
     3601 atgaaaccgc taaatccagg caatcctaat gaagccattc cagcaattac ataaattacg
     3661 gatataaaag gcattacttt caataaacct cctagtttgg tgatgtctct agtgtgggtt
     3721 cttccgtaaa tcatcccgat taaggcgaag aataaggcag tcataaatcc gtgggaaagc
     3781 gattgtaaaa tagctccgtt ccaagcagtt ttattcaaca tcaacaaggc gaacaacacc
     3841 attcccaagt gactcacaga ggaataagca ttaatgtatt tcaaatcggt ttgttttaac
     3901 gctccaaaag caccataaat gactcctgtt gccgataaaa tgatgaaaaa ccaagaccat
     3961 tcaatggctc ccaaaggcaa taaaaacata gcaatcctga aaacaccata acctcccaat
     4021 ttcatcaaaa cgcctgcatg cagcattgat accgctgttg gtgccgaagc gtgaccatca
     4081 ggtgaccaag tatgaaaagg aaataaagca cccaatacag caaagccaac aaaagtcaaa
     4141 gggaaaaata atttctgtgc ttcgaatgga atgtgcactt tagctatttc caaaatatta
     4201 aacgttaaag cgccaccatc ggcattcgaa ttgaaataaa ttcctagaac gcccaccatc
     4261 aaaacggctg aagctcccat cagcatcaaa gtcaacttca ttgccgaata ttctttgggt
     4321 cctgagcccc aaattccaat caacaaatac attgggatta cagcaatttc gtagaataca
     4381 aacattgtaa acaaatcgat ggaaatgaag aagccgtaaa ctcccgtggc caaaaccaaa
     4441 agagaaacaa agaattcttt gggcaaatcg tcggttttcc aagaaatgaa aacaccggct
     4501 aatactacta ttgcagtcaa caatagcaag gcaaccgaaa ttccatcaac tccaatggca
     4561 taatgcacat tgaaattttt aaaccaaaca taatcttgag tgaaaagcat cacgctttta
     4621 tcgaccgctc tttctctaaa ataagcaaaa agtaaattga tagccattcc gagttgaatg
     4681 aaacttccaa tcattgaaat caatcgagcc tgtttcaagc ctttggagaa aaccaaagct
     4741 aaaaccgtga gaacaggtac aattacaaaa agtgataaaa tatccatatt aattgatttg
     4801 ttatgtccat aaataaatga aaaccaaagc gacaactaca actcccgaaa caaaaaacat
     4861 agcataatct tgtaatcgac cggattgcaa tccttttatt ttttcagaaa tgatgaccgt
     4921 tttgtttcca atgccttcca tcgttccatc cacatatttc ttgtcgaact tggctattgg
     4981 tgctgaaatt agattgaaaa tgatttttct agtaatgaaa aggtagagtt cgtcgaaata
     5041 gaatttgtgg aaagtccatt tgtaaaacat cccaaaagca ttggaaaatc gttcggccaa
     5101 atcattttct tttttataga aaatccaagc cagaataatt ccgactaatc ccgttccaac
     5161 agcaacggct gctaatggta aatccaaatg accttcaaat cccgctttgt ctgcagttat
     5221 aaattcgctg aatggaataa atcctgctac aatgctcata acggccaaaa acaataatgg
     5281 aaaggccatt gacattggcg attcgtgcgg ttcgtgatgg tatttggttt ccttgcccca
     5341 aaagatgcca aaatacaatc ggaacatata aaaagcagtt aatcctgcaa cgaaaagtcc
     5401 aacaaaataa agcaattgat ttttttcgaa agcagcgact aagatttcgt ctttactcca
     5461 aaaccctgca aatggtggca ctccagcaat cgctaaagct gcgattaaga aagtaatatt
     5521 ggtgattggc atatacttgc gcaaacttcc catatctttc
//
LOCUS       DAOSNN010000182         5553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_14771_length_5553_cov_5.555111, whole genome shotgun sequence.
ACCESSION   DAOSNN010000182 DAOSNN010000000
VERSION     DAOSNN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14771_length_5553_cov_5.555111"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1514
                     /locus_tag="PLQ70_09280"
     CDS             84..1514
                     /locus_tag="PLQ70_09280"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HQE34844.1"
                     /translation="MFFNSLAFAVFLPIVFLLYWFVFGKTKSMQNAFLVVVSYYFYSC
                     WDWRFLFLLIFSTLLCYFTGIQIEKNTQKARKFWFWLSIILNLGFLGIFKYYNFFASS
                     LSEALNNIGLQSSPLLLDVILPVGISFYTFHGLSYVIDIYYKRIQSEKNVVDYALFVS
                     YFPLLVAGPIERATHLLPEIKVKRTFNFEKAKEGVYQVIWGLVKKVVIADTCATYANA
                     VFDNYSSMNSLSLILGAVYFAFQIYGDFSGYSDMALGMSKLFGLDLLRNFNYPYFSRD
                     IAEFWRRWHISLSSWFRDYLYIPLGGSRGSKIKQVRNVFIIFVVSGFWHGANWTFLAW
                     GFINAMYFLPSLLLGTNRNNMETAELHWDFNSIKVFFSILATFTLACVAWIFFRANSI
                     QIAFDYIRKIFTNGTFVSQFLPNQRYSYELLLLVLAFVLVEWNSRYKIEPISGKYSWL
                     KMTLCILAIIALGTYADYKEFIYFQF"
     gene            1514..2392
                     /locus_tag="PLQ70_09285"
     CDS             1514..2392
                     /locus_tag="PLQ70_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34845.1"
                     /translation="MKKFISLIAKISLTTLVLLVVLDWVYTSVYLHSSNRGKVEKVFN
                     SKDGNYDVVILGSSRANNHFVPELFEKKGLKTFNYGMSGAHLFEASLMLKLMTERHYK
                     IKTVLLEADLGLCNEKESEMIAAKFLPYIHHSEVIKNHFSGQADFNELYYIPFYRYID
                     FDALIGFREMYNTATDKPTNILDNQGYHPLGHKPGNMKNDIRALKPIRNKYYEEIKQI
                     CKQNNYNLITVMTPMCTNVKGLDYFEKANKIYPEIHNLENVVQGDQYFSSCGHMNDAG
                     ARMFTKIVIESFFEKK"
     gene            2402..3394
                     /locus_tag="PLQ70_09290"
     CDS             2402..3394
                     /locus_tag="PLQ70_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQE34846.1"
                     /translation="MANKTENTKIIRWGILGCGDVTEVKSGPAYQKTEGFKIEAVMRR
                     DAEKAANYAKRHHIDKYYSDADALINDPEIDAIYIATPPDTHHFYGLKVALVGKICCI
                     EKPLAPSYKESLEIYEAFKTKNIPLFTAYYRRTLPRFEQIKTWLDENKIGEVRSIRWN
                     LTKSASEQDLSGEYNWRTDAKVAPGGYFDDLASHGLDLFAFLLGDIKEVSGYSLNQQG
                     LYSAKDAITACWLHESGVTGNGNWNFGSQKREDIVEIAGTKGKINFSIFENDAIILSN
                     DEGETELFIEHPENVQLHHVERIREHLLGNSEHPSADFSAVKTSWVMDKILGNL"
     assembly_gap    3408..3507
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3508..3999)
                     /locus_tag="PLQ70_09295"
     CDS             complement(<3508..3999)
                     /locus_tag="PLQ70_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34847.1"
                     /translation="MKNLFKFSLVLGVALLTMNVHASDIDFSLGVKKEEGRIITIVLN
                     DAKKMDLSIYDVKNRLIHSENVASQKVINRTYDLKDLPEGTYYLEAESELKISRYKIS
                     VVGSTAVLSETAISDIYKPVFIEKDGLLTVSFINSQKSSATIKIYDHEDNEVYDSGLL
                     KDQK"
     gene            4184..5056
                     /locus_tag="PLQ70_09300"
     CDS             4184..5056
                     /locus_tag="PLQ70_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQE34848.1"
                     /translation="MKNILPALEIIEPSFGSSFSLTKYVDNDYSTATFWHYHPEIQLI
                     YVNGGSCKRQVGSHISYYTNGALILVGSNLPHCGFTDENTGNTKETVVHFRPDFLGEH
                     FFEIAEMKKIKQLFERAKAGIAFIGETKKRIGSKLETMADQMPFERLLTLLSILSELE
                     EATEFKVLNAEGFSMDLQMQDNDKINTIFNYVKNNFQEQITLEEVADLVSMTVPSLCR
                     YFKKITRKTFTKFVNEYRLVHASKLLAERPISITEVCFESGFNNFSYFNKSFQQFTGK
                     SASQYRKEHRSVLV"
     gene            complement(5138..>5553)
                     /locus_tag="PLQ70_09305"
     CDS             complement(5138..>5553)
                     /locus_tag="PLQ70_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013305016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34849.1"
                     /translation="FCGPTNIEAIFGLGMASEFAAAMFPTLFFVMLGDAADYSEFKNG
                     RRATGLIYSAGSFATKFGGGIAGAIIGLILGAFHYNGQDTASINGSIPGIIMLMSWVP
                     AIITIIAAGFMFLYPLNQKKMDEITVELNSRRLKE"
BASE COUNT         1696 a          965 c         1033 g         1759 t
ORIGIN      
        1 aatctgcggt ttttttaaat ataggacaat aaaccatttt gcccaaaagc gtgatgtttt
       61 ttgtctcata atacaactaa aatatgtttt ttaattcatt agcctttgct gtttttctac
      121 caatagtttt tctgttgtat tggtttgttt tcggcaaaac caaaagcatg caaaatgcat
      181 ttttggtggt ggtgagttat tatttttatt cttgttggga ttggcgtttc ttgttcttgt
      241 tgattttctc gacgctttta tgctatttca ccggaattca aatcgaaaaa aacactcaaa
      301 aagcccgtaa attttggttt tggctgagta tcatcctcaa tttgggcttt ctgggaatat
      361 tcaaatacta taattttttc gcttcttctt tatcggaagc cctgaataac attgggttgc
      421 aatccagtcc gcttttgctg gacgtgattc tgcctgttgg aatttctttc tatacatttc
      481 acggactgtc ctatgtcatc gacatttatt acaaaagaat ccagtccgaa aaaaacgtgg
      541 ttgattatgc cttgttcgtg agttactttc cgcttttggt ggctggtccc atcgaaagag
      601 ccacgcatct tttgcctgaa ataaaagtaa aaagaacctt caatttcgaa aaagccaaag
      661 aaggcgttta ccaagtcatt tggggcttgg tcaaaaaagt ggtcattgcc gatacttgtg
      721 cgacttatgc caatgccgtt tttgacaatt acagttcaat gaattccttg tcgttaattt
      781 tgggtgcggt gtattttgca ttccagattt atggcgattt ctcgggttat tcggatatgg
      841 ctttgggaat gtcaaaattg tttggtttag acttattgcg caatttcaat tatccttatt
      901 tttctagaga tattgccgaa ttttggcgtc gttggcacat ttcgttatcc tcttggtttc
      961 gagattattt gtacattccg ttgggcggaa gccgtggcag caaaatcaaa caagtgcgca
     1021 acgtattcat catatttgtt gtgagcggtt tttggcacgg agccaattgg actttcttgg
     1081 cttggggatt catcaatgcg atgtatttct tgccatcttt acttttggga accaaccgaa
     1141 ataatatgga aacggccgaa ttgcattggg atttcaattc gataaaagtg ttttttagca
     1201 ttttggccac ttttactttg gcttgtgtgg cctggatatt tttcagggcc aattccatcc
     1261 aaattgcttt tgattacatt cgcaaaatat ttaccaacgg aactttcgtc tcgcaattcc
     1321 tgcccaacca acgttacagc tacgaattac ttttactcgt tttagctttt gtattggtcg
     1381 aatggaacag ccgttacaaa attgaaccca tttctggaaa atacagttgg ctcaaaatga
     1441 cgttgtgcat tttggcgatt atcgctttag gaacctatgc cgattataaa gaatttattt
     1501 atttccaatt ctaatgaaga agtttatatc attaatcgcc aaaatttcac tcacgactct
     1561 agtgctttta gtggtgctgg actgggttta cacgagcgtt tatctacatt cttcgaatcg
     1621 tggaaaagtg gaaaaggttt tcaattccaa agacggaaat tatgatgtgg tgattttggg
     1681 ttcatcgaga gccaacaacc attttgtgcc tgaacttttt gaaaagaaag gactgaaaac
     1741 cttcaattat gggatgagtg gagcgcattt atttgaagct tcgctgatgt taaaactaat
     1801 gacagaacgt cattataaaa taaaaaccgt gcttctcgaa gccgatttag gactttgcaa
     1861 cgaaaaagaa tccgaaatga ttgctgcgaa attcttgcca tacatccatc attcggaagt
     1921 catcaagaat catttttcgg gtcaggccga ttttaacgag ctgtattata ttccgtttta
     1981 ccgttatatc gattttgacg cgctgattgg tttccgggaa atgtacaata ccgcaacgga
     2041 caaaccgacg aatattttgg ataatcaagg gtatcatccc ttgggtcaca aacccggaaa
     2101 tatgaagaat gacatccgag ctttgaaacc cattcgcaac aaatattacg aagaaatcaa
     2161 acaaatttgc aaacagaata attacaactt aattaccgta atgacgccaa tgtgcacgaa
     2221 tgtaaaaggt ttggattact ttgaaaaagc aaacaaaatc taccccgaaa ttcacaatct
     2281 ggaaaacgtc gtgcaaggtg accaatactt ttcttcctgc ggccatatga atgatgcggg
     2341 tgcgaggatg tttacgaaga ttgtaataga aagttttttt gagaagaaat aaaaaaatca
     2401 aatggcaaac aaaaccgaaa acacaaaaat aatccgttgg ggcatcttgg gatgtggcga
     2461 tgttaccgaa gtgaaaagtg gtccggctta tcaaaaaacg gaaggtttca aaattgaagc
     2521 cgtaatgcga agagatgccg aaaaagcggc caactatgcc aaaagacatc acatcgacaa
     2581 gtattattcg gatgccgatg ccttaatcaa cgatcccgaa attgatgcca tttacattgc
     2641 cactccgccg gacacgcatc atttttatgg tttgaaagtg gctttggtgg gaaaaatttg
     2701 ctgtatcgaa aaaccattgg caccgagcta caaggaaagt ctcgaaatat acgaagcttt
     2761 taaaaccaag aacattcctt tgtttacagc gtattacaga cgaactttgc cgcgtttcga
     2821 acaaataaaa acttggcttg acgagaataa aattggcgaa gtgcgttcta ttcgatggaa
     2881 tttgaccaaa tcggcttctg aacaagattt gtcgggagaa tacaattggc gcaccgatgc
     2941 aaaagtggct ccaggaggtt attttgacga tttggcgagc cacggtttgg atttattcgc
     3001 ttttcttttg ggagatatca aagaggtttc gggttatagt ttgaaccaac aaggattgta
     3061 ttcggccaaa gatgccatca ccgcttgctg gcttcacgaa tcgggcgtta caggaaatgg
     3121 caattggaat tttggaagcc aaaaacgtga agatattgtt gaaattgctg gaaccaaagg
     3181 caaaatcaat ttttccattt tcgaaaatga tgccatcatc ctttccaatg acgaaggcga
     3241 aacggaattg tttatcgaac atcccgaaaa cgtgcaattg catcacgtgg aacgaattag
     3301 agaacatctt ttgggtaata gcgaacatcc atctgccgat ttttcggcgg taaagactag
     3361 ttgggtgatg gataaaattc ttggtaattt ataaacacaa aaaaaaannn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnctt ttgatctttc aacaatccac tatcataaac
     3541 ttcgttatct tcgtggtcat aaattttaat agttgctgaa gatttttgag aattaatgaa
     3601 gcttactgtt aacagtccgt ctttttctat gaaaactggt ttgtatatat ctgaaatggc
     3661 ggtttctgat agcactgctg ttgacccaac gactgaaatt ttgtatctag aaattttcaa
     3721 ttcagattca gcttctaaat agtaagtccc ttcaggtaaa tctttcaaat catatgttct
     3781 attaataact ttctgagaag ctacattttc tgaatggatt aatctatttt ttacatcata
     3841 aatagacaga tccatttttt ttgcatcatt tagcacaata gttataattc ttccttcttc
     3901 tttttttaca cctagcgaaa aatcaatatc actagcatgt acgttcatag ttaataatgc
     3961 tactcccaac actaaactaa atttaaataa atttttcata attttaattt ttaggtttat
     4021 aatgatttat ttgaagcaaa gatattgtag agatgtgatg cgaaatagca ctttcttttc
     4081 atatttgtgt gatatattac cttttacgaa aacgaaatag gttaaaattg gtaatcatgt
     4141 gttaatttgg gttaattttg tactcattct aaataacagc attatgaaaa acatactccc
     4201 agcattagaa attatagaac catccttcgg aagttccttt tctttaacta aatatgtaga
     4261 taatgattat agtacagcaa ccttctggca ttaccatccc gaaatacaat taatatatgt
     4321 aaatggaggt tcttgcaagc gccaagtagg tagccatatt tcttattaca ccaatggagc
     4381 tcttattctt gttggaagta atttgcctca ttgtggtttt accgatgaga atacaggtaa
     4441 cacaaaagaa acagtggttc attttagacc cgattttttg ggagagcatt tttttgaaat
     4501 tgctgaaatg aaaaaaatca aacaactttt tgaaagagcc aaagcaggta ttgcttttat
     4561 tggcgaaacc aaaaaaagaa ttggaagtaa attagaaacg atggcagatc aaatgccttt
     4621 tgaaagattg ctgacattgc tttctatttt aagcgaactt gaagaagcca cagaattcaa
     4681 ggttttgaat gccgaaggat tctcgatgga tttacaaatg caggacaatg acaaaatcaa
     4741 cactatattt aattatgtga aaaataattt tcaagaacaa attacgctcg aagaagtagc
     4801 cgatttggtc agtatgacgg tgccgtcttt gtgtcgctat ttcaaaaaga taactcgaaa
     4861 aacgtttacc aagtttgtta atgaataccg tttggttcac gcgtcaaagc tgttggccga
     4921 aagacccatt agtattaccg aagtttgttt cgaaagcgga ttcaataatt ttagttattt
     4981 caacaaatca tttcaacagt ttacaggcaa aagcgcatca caatatcgca aagaacatcg
     5041 ttcggtttta gtataattct aatggaatta attcaaatta aaacccctta aaataatttc
     5101 gttttaaggg gttttctatt aaatagacat ttttaaatta ttcttttaat cttctagagt
     5161 ttaattccac agtaatttcg tccatctttt tttggttaag cggatataaa aacataaatc
     5221 ctgctgcgat aatggttata atggcaggaa cccaactcat tagcataatt atgcctggaa
     5281 tcgaaccgtt tattgatgct gtatcttggc cattgtagtg aaaagcacct aatattaaac
     5341 caataatagc tccggcaatt ccacctccaa attttgtagc aaatgatccc gccgaataaa
     5401 taagtccagt ggctcttcgt ccgtttttga attcagaata atcggctgca tctccaagca
     5461 taacgaaaaa tagggtagga aacatagcag cagcaaattc agatgccatt ccaagtccaa
     5521 aaatagcctc aatattggtt ggtccacaaa aaa
//
LOCUS       DAOSNN010000183         5441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15130_length_5441_cov_4.191608, whole genome shotgun sequence.
ACCESSION   DAOSNN010000183 DAOSNN010000000
VERSION     DAOSNN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15130_length_5441_cov_4.191608"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..695)
                     /locus_tag="PLQ70_09310"
     CDS             complement(129..695)
                     /locus_tag="PLQ70_09310"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQE34850.1"
                     /translation="MKKILVIDNYDSFTYNLVHYLEDLDCEVTVYRNDEFDIDEIAHF
                     DKILLSPGPGIPDEAGLLKPVIAKYAPTKSILGVCLGQQAIGEVFGGTLSNLDKVYHG
                     VSSMVKTSVNDELLFEGLGNEFEVGRYHSWVVDANLPDVLEATSFDENGQVMSLRHKT
                     YDVRGVQFHPESVLTPNGKKILENWLKN"
     gene            complement(740..2140)
                     /locus_tag="PLQ70_09315"
     CDS             complement(740..2140)
                     /locus_tag="PLQ70_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HQE34851.1"
                     /translation="MKAFTLNTHYKQILADTITPVSVYFKIRDKFPNSLLSESSDYHG
                     NDNSFSYICCNPIASIKIENETIFKTFPDGTSETISIDSKTDIPQVIQEFSGQFQSEK
                     NDFKFINNGLFGYISYDAVRYFEKVTIAKKDGETTIPDVYYAVYQNIIAINHFKNEAY
                     IFCHSVDGKNNIAEIEQLLQSRNIASYKFSRDGEGSSNLTDEEFKHNVALAKKHCHRG
                     DVFQLVLSRRFTQGFKGDEFNVYRALRSINPSPYLFFFDYGDFKIFGSSPEAQIIVKN
                     RKAEIHPIAGTFRRTGDDEKDAVLAKKLSEDKKENSEHVMLVDLARNDLSRHGHGVEV
                     EKYREVQFFSHVIHLVSKVTGHLHKKATTMQVVADTFPAGTLSGAPKHRAMQLIEDYE
                     KTNRNFYGGAIGFMDFEGNFNHAIMIRTFLSKNHKLHCQAGAGIVADSSEENEMQEVY
                     NKLLALNKALDLAETI"
     gene            complement(2443..3006)
                     /locus_tag="PLQ70_09320"
     CDS             complement(2443..3006)
                     /locus_tag="PLQ70_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQE34852.1"
                     /translation="MKNLKTIALAFVAFATLSVSAQTKKIDVKTSTIEWVGKKVTGQH
                     NGTVNFKDGAVVLKGKKLVGGTFTVDMTSLTATDLTGEYLGKLNGHLKADDFFGTDKF
                     PTATLVIKKIGAKSANIYTTTADLTIKGITKPVTFDITVNGNTATTAFNVNRTIYGIQ
                     YGSKSFFDSIGDKAINDEFELKVTLKF"
     gene            complement(3036..3668)
                     /locus_tag="PLQ70_09325"
     CDS             complement(3036..3668)
                     /locus_tag="PLQ70_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQE34853.1"
                     /translation="MSTFLEHQNWRYATKKFDSTKKISTEDLNTLKEAIRLSSSSYGL
                     QLYKVFIIENPELRAKIQPAAWGQTQIVDASHLIVFANQTNITEADIDGYLKNVAETR
                     NIPETALSGYGDFMKGGILSRSDDDKANWTSKQTYLALGNLLNAAAELKIDVTPMEGF
                     VPAQVNEILGFNELGLNASLIAPIGYRSQEDDTQHYAKVRKSNEELFITL"
     gene            complement(3688..4143)
                     /locus_tag="PLQ70_09330"
     CDS             complement(3688..4143)
                     /locus_tag="PLQ70_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQE34854.1"
                     /translation="MKIEDVIKSTVPMENSKKIILNVMYTQNILAEKFNEILKPYDIS
                     GEQYNVLRILRGQKGNPANMCVIQERMLAKTSNTTRLVDKLLLKEFVTREVCPENRRK
                     IEVTITQKGLDVLKELDPKVTEHEIAFSKNLTDDEIVVLNQLLEKYRNQ"
     gene            4562..4867
                     /locus_tag="PLQ70_09335"
     CDS             4562..4867
                     /locus_tag="PLQ70_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQE34855.1"
                     /translation="MNLTQEDWVSQLKADDNSVILDVRTEAECDEGMIANAINIDIYE
                     GQGFVSKIEDLDKTKNYYVYCRSGARSAKACEVMNSLGIENTYNLLGGILEWDGEVV"
     gene            5000..>5441
                     /locus_tag="PLQ70_09340"
     CDS             5000..>5441
                     /locus_tag="PLQ70_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQE34856.1"
                     /translation="MNTIPQEFQISSLLTQDTYLVNGELKKWSGETTPVYSTISSTEN
                     YAPTLLGSIPFMGEKEALEVLESASSAYNNGQGLWPTMKVADRIKCMEKFVAQMKTTR
                     TEVVKLLMWEIGKSLGDSEKEFDRTVEYIYDTIEHYKQLDRNSAH"
BASE COUNT         1644 a          864 c          975 g         1958 t
ORIGIN      
        1 ttacaagaga tgaagccgaa aataaaaagg caaagtataa gatggatttt gactgttttc
       61 atttatttac cgcaaagacg caaaggcgca atttttaata ctgaacacta aaaactgatt
      121 actgaatact agtttttaag ccagttttct aaaattttct ttccgtttgg agtcaaaacg
      181 ctttctgggt gaaattgaac gccacgaaca tcatacgttt tgtgacgcaa agacattact
      241 tgtccgtttt cgtcgaaaga agtggcttcc aaaacatctg gtaaattggc gtcaaccacc
      301 cacgaatggt agcgtcccac ttcgaattcg tttcctaaac cttcaaataa aagttcatca
      361 ttaacagatg tttttaccat tgaggaaact ccgtgataca ctttgtccaa attagataat
      421 gtcccgccaa aaacttctcc aatagcctgt tggccaaggc atacaccaag aatacttttg
      481 gttggagcat atttagcgat tacaggtttc aataatcctg cttcatcagg aattccaggt
      541 cctggagaaa gtaatatttt gtcgaaatga gcaatttcgt caatatcaaa ttcatcgttt
      601 ctgtacacgg taacttcaca atccaaatct tcaaggtaat gcaccaagtt gtaagtgaag
      661 ctgtcgtaat tgtctataac tagtattttt ttcattttgt tatttttgtt ttagtgacaa
      721 gtcgtgactt gtccttacat tatattgtct ctgctaaatc cagcgcttta ttcaacgcca
      781 ataatttatt gtaaacctcc tgcatttcgt tttcttcgct agaatcggct acaattccgg
      841 ctccggcctg acaatgcaac ttatgatttt tacttaagaa agttcgaatc ataatagcgt
      901 gattgaaatt gccttcaaaa tccataaaac cgatagcacc tccgtagaaa ttacgattgg
      961 ttttttcgta atcttcgatt aattgcatcg ctctgtgttt gggcgctccg cttaatgttc
     1021 ctgccgggaa tgtatcggca acgacctgca ttgtggtggc ttttttatgt aaatgtccag
     1081 tcactttgga aaccaaatgg attacgtgag agaaaaattg aacttctctg tatttttcca
     1141 cttccacacc gtgaccatga cggcttaaat cgtttctcgc taagtcaacc agcattacgt
     1201 gttcgctgtt ttcttttttg tcttcggata attttttggc caaaacggca tctttttcgt
     1261 catcgccagt tcttctgaaa gttcctgcga tggggtgaat ttcggctttg cggtttttga
     1321 caataatctg ggcttctgga gaagaaccga atattttgaa atcgccataa tcaaaaaaga
     1381 acaggtaagg agaggggttg atgcttctca aagctctgta cacattgaat tcatcacctt
     1441 tgaaaccttg ggtaaatctt ctggacaata ccaactggaa cacgtcacct ctgtggcagt
     1501 gtttttttgc taaagctaca ttgtgtttga attcttcatc agttaaatta gaagaacctt
     1561 cgccatctct tgagaattta tacgaagcta tatttcggga ttgcaacaat tgttcaattt
     1621 cagcaatgtt gttttttccg tctacactgt ggcagaaaat ataagcttcg tttttgaaat
     1681 gattgatggc aatgatgttt tgatacactg cataatacac atcgggaatg gtggtttctc
     1741 cgtctttttt ggcgatagtt actttctcaa aataacgaac agcatcataa gaaatataac
     1801 caaacaatcc attgttgatg aatttgaaat cattcttttc ggattggaac tgacctgaaa
     1861 attcctgaat tacttgagga atatccgttt ttgaatctat cgaaatggtt tcggaagttc
     1921 cgtcaggaaa tgttttgaaa atggtttcgt tttcgatttt gatggaagca atcgggttgc
     1981 aacagatgta ggaaaaacta ttgtcgtttc cgtgataatc actactttcc gaaagcaagc
     2041 tgttggggaa tttgtcacga attttaaaat agacgcttac tggggtaatc gtgtcggcaa
     2101 ggatttgctt gtaatgtgta tttaaagtaa atgctttcat tttgttttta ttatttgatg
     2161 gagattttta caactcctgt atttttgaaa tagaaaaagg cttgtcgtga tgacaagcct
     2221 tttatatgat agtttatact acaggtgctt tgttcacgac gtttgacgta aattgttcca
     2281 ccaccaagta ttgtttaaaa tcgttttcat tcgacaaata tagaaatcaa atttgaaaat
     2341 ccaaacagaa taaattaaaa atactttttt ttgtcttttt gttaaaaaag taaccctgac
     2401 aattgcttgt cagggtttac taaaatttat tttaatttga aattagaatt tcaaagtcac
     2461 tttcaattcg aattcatcat tgattgcttt gtctccaatg ctatcaaaga aagattttga
     2521 accgtattga attccgtaaa tagttctgtt tacattgaaa gctgttgtag cagtatttcc
     2581 gtttacagtg atgtcaaaag ttaccggttt agtgattcct ttgatagtca aatcagcagt
     2641 tgtagtgtaa atgttggctg atttagcacc aatttttttg ataaccaagg ttgctgttgg
     2701 gaatttatcg gttccaaaaa agtcgtctgc tttcaagtgt ccgttcagtt tccctaaata
     2761 ttcaccagta agatcggtag cggtcaatga agtcatgtca actgtgaaag ttccacccac
     2821 caattttttt ccttttaata ctacggctcc gtctttgaaa tttacggtac cattatgttg
     2881 tccagttact ttttttccaa cccattcgat ggtacttgtt ttaacatcaa tttttttggt
     2941 ttgagctgat actgaaaggg tagcgaaggc tacgaatgct aatgcaattg tttttagatt
     3001 tttcataatg tttgaatttg tttttggatt tataattaaa gtgtgataaa taattcttcg
     3061 tttgattttc taacttttgc gtaatgctgg gtgtcatctt cttggcttct gtaaccgata
     3121 ggcgcaatta atgaagcgtt caatcccaat tcgttgaaac ctaatatttc gtttacttgg
     3181 gcaggaacaa aaccttccat tggcgtcacg tcaattttta gttccgctgc ggcattcaat
     3241 aagtttccaa gtgctaaata ggtttgttta gatgtccaat tggctttatc gtcatctgat
     3301 ctgcttagga ttccaccttt cataaaatcg ccgtaacctg ataatgctgt ttctgggatg
     3361 tttctagttt cggctacgtt tttcaaataa ccatcaatat ccgcttctgt tatattagtt
     3421 tgatttgcga aaacgataag gtgagaagca tccacaattt gggtttgacc ccaagcagca
     3481 ggttgaattt tggctcttaa ttcagggttt tcaatgataa aaactttgta aagttgtaat
     3541 ccataagaag aagaacttaa acgaatggct tcttttaatg tatttaaatc ttcggttgag
     3601 atttttttgg tggagtcgaa tttctttgtc gcgtatctcc agttttggtg ttctaaaaaa
     3661 gtactcatat tgtatttgat atttaattta ttgatttcta tatttttcta ataattggtt
     3721 taataccaca atttcgtcat ccgttaagtt tttcgaaaaa gcgatttcgt gttcggttac
     3781 ttttggatct agttctttca aaacatctaa ccctttttga gtgatggtga cttcgatttt
     3841 gcgtcggttt tctgggcaaa cttctcttgt aacaaattct ttcaacaata atttatcaac
     3901 taaccgggtg gtattacttg tttttgccag cattcgttct tgaatcacac acatattggc
     3961 tggatttcct ttttgacctc tcaagattcg caatacatta tattgttctc ctgaaatatc
     4021 gtaaggtttc aaaatttcat taaatttttc tgccaaaata ttttgtgtgt acatcacatt
     4081 caggataatt tttttggaat tttccatcgg tacagttgat tttataacat cttcaatttt
     4141 catatttgta tgtatgttat ttgttggtac aaatgtaata tgttttttag ttgtatatac
     4201 aaatgtttga gttaattttt tgttaaaaca attctaagaa aaattaatga ttagtagata
     4261 ttgtgtttat gtgcttgatt tttaatgttt taaaatgatg tttaaaaaat aattacaaca
     4321 tataattatt gattatttat aataattaaa aattttaatt agagataaaa acaaataaac
     4381 tgtaaattag ctgcataata gttagttttt cgagtagttg ttaatgaata ttacagtaga
     4441 attaaaattt tattgttttt ttatttatac taaaacgttt tctgtaaaaa attatccaat
     4501 taacagatag tgccataaat gttgggcaaa aactttgtaa ttttaccaaa aacaatatta
     4561 tatgaattta acacaagaag attgggtttc tcaattgaaa gccgatgata attcagttat
     4621 tctggatgtc agaaccgaag ccgagtgcga tgaaggaatg attgctaatg ctatcaatat
     4681 tgatatttat gaaggtcaag gatttgtatc aaaaattgaa gacttagaca aaaccaaaaa
     4741 ttattatgtc tattgccgct ctggagctag aagtgctaaa gcttgcgagg ttatgaatag
     4801 ccttggtatt gaaaatacct ataatttatt aggtggaatt ttagaatggg atggggaagt
     4861 agtttaaata tttaaaagtt taaatgttta agagtttaaa ggtttgaatg tttaaggttt
     4921 gaatgtttaa ggtttgaatg tttaaggttt gaatgtttta atcggttaaa caaattaatg
     4981 aatcaacaac aaaaaaagaa tgaatacaat accacaagaa tttcaaatta gcagtttact
     5041 tactcaagat acctatttag ttaatggcga attaaaaaaa tggtcaggcg aaacaactcc
     5101 tgtttattcg acaatttctt caacagaaaa ttatgcacct actttattag gaagtattcc
     5161 ttttatgggt gaaaaagaag cattggaagt gctagaatca gcttcttctg cttataataa
     5221 cggacaaggt ttgtggccta ctatgaaagt agctgatcga attaaatgta tggaaaaatt
     5281 tgtcgctcaa atgaaaacta ctcgaacaga agtggttaag cttttgatgt gggaaattgg
     5341 aaaatcatta ggagattctg aaaaagagtt cgatagaacg gttgagtata tttatgatac
     5401 catcgaacat tacaaacaat tggatagaaa tagtgctcat t
//
LOCUS       DAOSNN010000184         5421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15201_length_5421_cov_4.359486, whole genome shotgun sequence.
ACCESSION   DAOSNN010000184 DAOSNN010000000
VERSION     DAOSNN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15201_length_5421_cov_4.359486"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLQ70_09345"
     CDS             complement(<1..283)
                     /locus_tag="PLQ70_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34857.1"
                     /translation="MKTDNPIKNLEQAKAFFVSMGCSGFHMMRENHLKYDEYRALEID
                     PSIESEWIKEVFEKKLEKFNTISPNDYGWQLDNLASLINRKESHLEKLLE"
     gene            complement(351..2783)
                     /locus_tag="PLQ70_09350"
     CDS             complement(351..2783)
                     /locus_tag="PLQ70_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HQE34858.1"
                     /translation="MSSKKPAFFSALLLIAFSCLFAQERTTIELNKGWKFSKGNNEKA
                     FENNFNDSKWQAVSVPHDWAIYGPFDKEIDKQVTAIVQNGEKVATEKTGRTGALPYIG
                     EAWYRNQFSVPNLDKNKKVILLFEGAMSEPKVFLNGKKVGEWNYGYNYFYFDVTDFLV
                     ANGKNNLAVHLSNVGESSRWYPGAGLYRKVKLIVKNKESINQWGTFVTTPIVTDDLAK
                     VNIKTKVTGENLRIVTKILDASGNEVANNKSDAIFGNEFEQNIAVKNPKLWSPETPYL
                     YTAISQIYVGDVLKDETSTRFGIRTIKFEPNKGFSLNGKVRKFKGVCLHHDLGPIGTA
                     INKAALRRQLSILKDMGCDAIRSSHNMPSLEQLELCDEMGFMFLAESFDEWAKPKVKN
                     GYNRFFTTDAEKDVVNLIHATRNHPSIVMWSSGNEVPDQWGAEGVKRAKWLQEIFHRE
                     DPTRPVTVGMDQVKATMESGFGAIMDIPGLNYRLPLYDEAFKKFPQGFILGSETASTV
                     SSRGVYKFPVVKGHSKQYPDFQSSSYDLEYCSWSNVPDDDFVLQDDKPWVIGEFVWTG
                     FDYLGEPTPYDEMWPSRSSYFGMCDLAGIPKDRYFLYKSRWNTTDKTLHILPHWNWEG
                     REGQTTPVFVYTNYDSAELFVNGKSMGIQKKNNATPQNRYRLMWMDVKYEPGTIKVVA
                     LDKDGKAVAEESIHTAEKPYQIKLETDRSTLEANGEDLSYVTVSVVDKNGNPCPTATQ
                     QLNFSVSGKGTYRAACNGDATSLEQFHLPTMKLFSGKLVVLVQSTEEAGKLELTVTGK
                     GLKSGKIELESK"
     gene            complement(2825..3151)
                     /gene="rhaM"
                     /locus_tag="PLQ70_09355"
     CDS             complement(2825..3151)
                     /gene="rhaM"
                     /locus_tag="PLQ70_09355"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HQE34859.1"
                     /translation="MKNNTIRNAFKMKLKPGFEVEYKKRHDTIWPELQSLLSETGIQD
                     YSIFLDEETLILFAVQKISPDFDFDYLPNHPIVKKWWAYMADIMDTNPDNSPVAKPLT
                     EVFHAD"
     gene            complement(3199..4635)
                     /locus_tag="PLQ70_09360"
     CDS             complement(3199..4635)
                     /locus_tag="PLQ70_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQE34860.1"
                     /translation="MVLQQNSKVNLWGWAAPNEKINIQLGWLNSKLEITTKPDGTWKT
                     AVETPKGSEKPYTIVLEASNKIILKNVLIGEVWICSGQSNMYFPVGKEDKTWKTGVVN
                     YEEEIKNANFPNIRLFTVLTKASPKPLDDVTGSWAECSPESIKNFSAVAYFYGRDLYQ
                     KLNIPIGLISSSWGGTKAEAWTAQNVLEENPDFLSILENDAKNEKTYQEKLEKYYSDF
                     KTERIANNNDLTKSEVKKPKKEDNKTSYVLYNAMLYPLTNYTIKGAIWYQGESNAESA
                     ILYRSLFPAMVKNWRNVWNQGDFPFYYVQIAPHKGQNAFIREAQLLSLKTIPSSGMVV
                     TTDVGDANNIHPIDKQTVGHRLALIARAKTYNENKLVYSGPIYNQMKIKKDHIQLFFD
                     YADSGLVKTGDVLKEFEIAGDDKKFFPADAKIDGKTIIVSSKEVKNPVAVRFAWKAVP
                     EANLFNKENLPASPFRTDDWVIEPTKKE"
     gene            complement(4873..>5421)
                     /locus_tag="PLQ70_09365"
     CDS             complement(4873..>5421)
                     /locus_tag="PLQ70_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HQE34861.1"
                     /translation="KAGVGTYADALPYINKVRTRGAYKAGENRAAYADGSASWTVALN
                     AGIPSSYIAENSYYESNNIATTVNTATSLTISSISTLPAEDQAIITKLGYSTDYDRML
                     CLVLNEKTRELCGEFHRWEDLSRTKTLVARAKAYNPDAAPNIQDYHSLRAIPQTFLDA
                     IYTEGRPLTAAEKKAMQNPGYN"
BASE COUNT         1487 a         1028 c          998 g         1908 t
ORIGIN      
        1 actctaataa tttttcaaga tgggattctt ttcgattaat caaagaagct aaattatcta
       61 attgccatcc ataatcattc ggagaaattg tattgaattt ttcaagtttc ttttcaaata
      121 cttcttttat ccattcagat tcaatactcg ggtcaatttc aagtgctcta tattcgtcat
      181 attttaagtg attttcacgc atcatatgaa aaccactaca ccccattgat acaaaaaatg
      241 cttttgcttg ttctaaattt ttaattggat tgtctgtttt catcgtttgc ttttaatcaa
      301 taaatactag caattataat tttatttcaa ttacttttta aatctcaaaa ttactttgat
      361 tccaactcta tttttccgct tttcaatcct tttccggtta cggtcaattc cagttttcct
      421 gcttcttcgg tagattgaac caaaaccact aatttaccgc taaataattt cattgttggc
      481 aaatgaaatt gttccaatga agtggcatcg ccgttgcaag ctgctctgta agttcctttt
      541 ccgcttacgc taaaattcaa ttgttgcgtg gcagttggac acggatttcc gtttttatca
      601 accacagaaa cagtaacgta tgataaatct tcgccattgg cttcgagcgt gcttctatcg
      661 gtttcgagtt ttatttggta tggtttttca gcggtgtgaa tgctttcttc tgccactgct
      721 tttccgtctt tatccaaagc cacgactttg attgttccgg gttcgtattt gacatccatc
      781 cacatcaaac ggtagcgatt ttgtggcgtg gcgttatttt ttttctggat tcccatactt
      841 ttgccattga caaaaagttc ggcactatca taattggtgt acacaaaaac aggcgttgtt
      901 tgtccttcac gaccttccca attccaatgt ggcaaaatgt gtaaggtctt gtcggtcgtg
      961 ttccaacggc ttttatataa aaaatatctg tcttttggaa ttccagccaa atcgcacatt
     1021 ccaaaataag aacttctaga aggccacatt tcgtcataag gtgttggttc gcccaaataa
     1081 tcgaaacctg tccaaacaaa ttcgccaatt acccaaggtt tgtcatcttg taacacaaaa
     1141 tcatcatcgg gaacattcga ccaactgcaa tattctaaat cataggaaga cgattgaaaa
     1201 tcgggatatt gcttggaatg tcctttaaca actggaaatt tgtaaactcc acgagaactc
     1261 acggtcgaag ctgtttcgga acccaaaata aatccttgcg gaaatttttt gaacgcttcg
     1321 tcgtataaag gcaaacgata attcagtccg ggaatatcca ttatggctcc aaaacccgat
     1381 tccattgtgg cttttacttg atccattcca acggttacag ggcgagttgg gtcttcgcga
     1441 tggaagattt cctgcaacca tttggcacgt tttacgcctt ctgctcccca ttgatctggg
     1501 acttcgttac cagaactcca catcacgata gacggatggt tgcgagtggc gtgtatcaaa
     1561 ttgaccacgt ctttttcggc atcagtagta aaaaaacgat tgtatccatt ttttactttt
     1621 ggctttgccc attcgtcaaa actttcggcc aggaacataa atcccatttc gtcacacaat
     1681 tcgagttgtt ccaatgacgg catattgtgg gaactgcgaa tggcgtcgca acccatatct
     1741 ttcaaaatgg acaattgtct gcgcaaagcg gctttgttga tagctgttcc aattggacct
     1801 aaatcgtggt gcaaacaaac gcctttgaac ttgcgtactt ttccattcaa actaaaacct
     1861 ttgttgggtt cgaatttgat ggttctaata ccaaatcttg tgctggtttc gtctttaaga
     1921 acatcgccaa cataaatttg ggaaatggca gtatagaggt aaggcgtttc tggactccat
     1981 aattttggat tttttacagc aatattttgt tcgaattcgt tgccaaaaat tgcatctgat
     2041 ttattgttgg ctacttcatt tcccgaagca tccagaattt tggtgacgat gcgaagattt
     2101 tcgccagtaa ctttggtttt gatatttact tttgccaaat catcagttac gattggagtg
     2161 gtcacgaaag tgccccattg attgatgctt tccttgtttt tgacaatcag tttcactttt
     2221 cggtacaaac cggctcctgg ataccaacga gaggattcgc caacattaga caaatgaact
     2281 gccaaattgt tcttcccatt agcaaccaaa aaatcggtaa cgtcaaagta aaaataattg
     2341 taaccatagt tccattcgcc tacttttttg ccattcaaaa acactttggg ttcactcatc
     2401 gccccttcga aaaggaggat tactttcttg tttttatcca aattgggaac cgaaaattga
     2461 tttctgtacc aagcttcacc gatgtaaggc aatgcgccag ttcgacccgt tttttcggtg
     2521 gccacttttt ctccattttg aacaatggcg gttacttgct tgtcgatttc tttgtcgaaa
     2581 ggcccgtaaa tagcccaatc gtgcggaacg ctaacagctt gccatttcga atcattaaaa
     2641 ttattttcga aagctttttc gttgttgcct ttggagaatt tccatccttt atttagttcg
     2701 attgttgttc gttcttgggc aaaaagacaa gaaaacgcaa taagtagcaa tgcggaaaaa
     2761 aatgcaggtt ttttagaact cataattaag aatttgatta aattttaaag gtactaaaaa
     2821 ttatttaatc ggcatgaaaa acttctgtta ggggtttggc tacaggagaa ttatcaggat
     2881 tagtatccat aatatcggcc atataggccc accatttttt tactattgga tgattgggca
     2941 aataatcaaa atcaaaatca gggcttatct tttgaaccgc aaagagaatt agcgtttctt
     3001 catctaaaaa gatgctgtaa tcttgaattc ctgtctcaga aagcaaggat tgtagttcag
     3061 gccaaatggt atcgtgtctt tttttatatt ccacttcaaa gccgggtttt agtttcattt
     3121 tgaaggcatt tcggatggtg ttatttttca ttatttctga tgttttttgg ttaaaaaaag
     3181 gggggtatgc taatgaattt attctttttt ggttggttca atcacccaat cgtcggttct
     3241 aaaaggagaa gcaggtaagt tttctttatt gaataaattg gcttctggta cggctttcca
     3301 agcaaaacga acggctactg gatttttaac ttctttagac gaaacgataa tggttttacc
     3361 gtcaattttg gcatcggctg ggaaaaactt tttgtcatca cctgcaattt caaattcttt
     3421 tagcacatct ccagttttca ccagtccaga atcggcataa tcaaaaaaca gttggatatg
     3481 gtcttttttg attttcattt gattgtaaat aggaccggag taaaccaatt tattttcgtt
     3541 ataggtttta gccctagcga ttaatgccaa tcgatgtccc acagtttgct tgtctattgg
     3601 atgaatattg ttggcgtcac ccacatcggt agttacgacc attcctgaac tggggatggt
     3661 tttcaaggac aacaattgtg cttctctaat gaatgcattt tgccctttgt gaggagcgat
     3721 ttgaacatag taaaatggaa aatcgccttg attccatacg tttctccaat ttttgaccat
     3781 tgctggaaat aggcttcggt acaaaatggc tgattcggca ttgctctcgc cttgatacca
     3841 aattgcccct ttgatagtat aattagtcag aggatataac atggcattat acaaaacata
     3901 ggaagtttta ttgtcctctt ttttaggttt ttttacttcc gatttagtca aatcgttgtt
     3961 gttggcgatt cgttctgttt taaaatcaga atagtatttt tctagtttct cttggtatgt
     4021 tttttcgttt tttgcatcgt tttcaagaat ggataaaaaa tctggatttt cttctaaaac
     4081 attttgtgca gtccaagctt cggcttttgt tcctccccaa gaagaagaaa ttaatccaat
     4141 ggggatgttt aatttttggt atagatctct tccataaaaa taggctacag ccgaaaaatt
     4201 tttgatgctt tctggggaac attctgccca acttccggta acatcatcta atggttttgg
     4261 tgaagctttg gttaaaacgg taaatagtct gatattggga aaattggcgt ttttgatttc
     4321 ttcttcgtaa ttcacaacac ctgttttcca agttttatct tcttttccga ctgggaaata
     4381 catattggat tgccctgaac aaatccaaac ttcgccaata agaacgtttt tgagaattat
     4441 tttattagaa gcttcaagaa caatggtgta tggtttttcg cttccttttg gtgtttctac
     4501 tgctgttttc catgttccat caggttttgt tgtgatttct aactttgaat ttagccagcc
     4561 taattggatg ttaatttttt cgtttggtgc tgcccaaccc cataaattca cttttgaatt
     4621 ttgttgtaaa accatgttat tacctactaa agagggtaat ttgatttgag atattgctac
     4681 tagatttgtt gttagaacaa gtagaaggac tagaactttt ttggtgcaat tcattattag
     4741 ttttaatttg atagtaagag tttaaaggta aaggaattta actgtttagc tatcctgtac
     4801 tttcctgcaa aaaaaaagtg ctatagcagt ttgctatagc acttgattgg tttattttgg
     4861 ttatggttat aattaattgt atccagggtt ttgcattgct tttttctcag cagctgtcaa
     4921 cggacgacct tcggtgtaaa ttgcatctaa gaaagtttga ggaatagcac gcaaactgtg
     4981 ataatcttga atatttggag ctgcatcagg gttgtaagct tttgctcttg ctaccaatgt
     5041 tttagttcta cttaggtctt cccaacggtg aaactctcca caaagttctc tagttttctc
     5101 gtttaacact aaacataaca ttctgtcata atcagttgag taacctagtt ttgtaattat
     5161 tgcttgatcc tctgcaggta aagtgctaat gctagaaata gttaagctag ttgcagtatt
     5221 aactgtagta gcaatattgt tagactcata atatgagttt tcagcaatat aagaactcgg
     5281 aatacctgca tttaatgcaa ctgtccatga agctgaacca tcagcataag cagcacgatt
     5341 ctctcccgct ttgtatgcac cacgtgtacg aactttgttg atgtaaggta atgcatctgc
     5401 ataagtacct actcctgctt t
//
LOCUS       DAOSNN010000185         5361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15396_length_5361_cov_4.006408, whole genome shotgun sequence.
ACCESSION   DAOSNN010000185 DAOSNN010000000
VERSION     DAOSNN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15396_length_5361_cov_4.006408"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLQ70_09370"
     CDS             complement(<1..740)
                     /locus_tag="PLQ70_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HQE34862.1"
                     /translation="MKKVFYIFGFLISCFAWSQSKERTAEFRKEVPLNSSWETIVLDK
                     LPEQEESFVQNPKVDAQWQKVGVPHNWDQYYGFRRTKHGNLHGTAWYHKSLQLDKQDA
                     GKRLFLFFEGVSSYATVWVNGKKVGEHKGGRTTFTVDITKAVSFEKGNDILVKAAHPS
                     FIADLPWVCGGCSGEWGFSEGSQPMGIFRPVTLVVTDEVRIQPFGVHIWNDKTTSKEK
                     AVLNITTEIKNYGKFNRNLSIVNTPFDK"
     gene            complement(745..>3054)
                     /locus_tag="PLQ70_09375"
     CDS             complement(745..>3054)
                     /locus_tag="PLQ70_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34863.1"
                     /translation="EMVLRGSCIGMQKTAYLPGRDVYEYPYTPETFPWFYDKKLWTKY
                     LDMMVDNRMNSLYLWNGHPFASLVKLKDYPFALEVSEEDFKKNEEIYKYLTVEANKRG
                     IWVIQMFYNIIVSKPFAEHYNIKTQDRSRSITPLLSDYTRKSIAAFIEKYPNVGLLVC
                     LGEAINTVDDDVEWFTKTIIPGVQDGLKALGRTDEPPIILRSHDTDAPLVMEKSLPLY
                     KNLYTESKYTGESLTTYAPGGPWGETHRQLSALKSIHIENVHILANLEPFRYGSPDFI
                     QKTVKAMHNIHGANALHLYPQASYWDWPYSADKTKTGERLLEMDRDWIWYKAWSRYAW
                     DSKRDRNEEVKFWDKDLAAKYGTDEEAGNNILKAYEEAGEIAPKTLRQFGITEGNRQT
                     LLLGMFESQLVNPAKWRVYPGFHESCGPITELLLDYAKKEWNHEAHAGETPPQIISEI
                     TQHGRLAVEAIDKAEAKVTKDKEEFLRLKNDMHAYKAFADFFSEKVKAAMLVLRYSYS
                     NDISDLDKALPHLEKSIEEYQTLVNLTKDSYLYANSMQTAQRRIPIGGDDGNNKTWAE
                     LLPHYEREMANFKRNLALLKLSKDGKIATKEGKPWQTAEVTLLSESKGTYAVKNGTKV
                     YGTPISELTKIAPELQNLKGITFDETSQNESGTHLKFKNTKAVKLVVGYFNSDQKRFL
                     LPPSLETDAAGNAYGQAEVILASAMNLKELPRVNIHTYTFQPGENKLDLGKGKVLILG
                     FIDANQTITTRDVGYIDAGEKGAIDWLFY"
     assembly_gap    3055..3154
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3155..3632)
                     /locus_tag="PLQ70_09380"
     CDS             complement(<3155..3632)
                     /locus_tag="PLQ70_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HQE34864.1"
                     /translation="MKYLSLLFLCFYVSVSSAQNITIVRDANAPRATFGAEKLSEIAT
                     TKGFKVVFTDKAPKKSKDKVIVIGEKGTDFWKQNSKNAKIDDSQLTKKEGFQICTQKN
                     IIYIEGTDATGALYGTMELVDRIKTSGEIPSEINIDDSPEMVLRGSCIGMQKTAYLP"
     gene            complement(3767..>5361)
                     /locus_tag="PLQ70_09385"
     CDS             complement(3767..>5361)
                     /locus_tag="PLQ70_09385"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HQE34865.1"
                     /translation="WNPVLYQIPKGDLLLFYKIGPSPSTWWGMLLRSSDGGETWSKPE
                     KLPEGFLGPIKNKPVLLANGTLLIPSSIEGKGGWHLRMESTPDFGKTWIMGDTIGKKQ
                     DTINAISAIQPSILFHKKGKLQAIGRTRNRHLFSTFSNDNGKTWSALELLNLPNNNSG
                     TDAVTMKNGEHALIYNHVLPYGDDPKGVRTPLNIAVSKDGINWDASLVLEDSRIGQYS
                     YPAIIQGSDGMLHCVYTWRRQRIKYVKIDPSKLVSFPIVNGVWPGPTKKIYQVAVCDW
                     MILKRQKLGAFELAKSINSDGIELDMGGLGNRETFDSKLGDPVERQKFLDKSKETGVE
                     ISSIAMSGFYAQSFAERETVEKMVNDCIETMKNTNVQIAYLPLGTQCDLAKHPELRAK
                     VIERLRWAGEQVNKIGGVIAVETSLDAAGDKKLLEEIGNRNIKISFNFANAVDNGRDI
                     SKELKTLGRDNIAQIHASTTDGVWLQNNKAVNLPEIKKTLDKMQWGGWLIIERSRDVN
                     QVHDVKANYGANVEYLKSIFKN"
BASE COUNT         1386 a         1152 c          940 g         1783 t
ORIGIN      
        1 tctttgtcaa aaggcgtgtt tacaatgcta aggtttctat tgaatttacc gtagtttttg
       61 atttcggtcg taatattcaa aacggctttt tctttggaag tggttttgtc gttccaaatg
      121 tgaacgccga aaggttgtat tctaacttca tcggtaacga ccaaagttac gggtctaaaa
      181 atccccattg gctgggagcc ttcggaaaat ccccattcgc ctgaacaacc tccgcaaacc
      241 caaggcaaat cggctataaa tgacggatgg gccgctttga cgagtatgtc atttcctttt
      301 tcgaacgaaa cagccttggt gatatccacc gtaaaagtgg ttcttccgcc tttgtgttcg
      361 cccacttttt tgccgtttac ccaaaccgtt gcataggagc ttacaccttc aaaaaaaagg
      421 aaaagtcttt tccctgcatc ctgtttatct aattgcaatg atttgtgata ccaggcggtt
      481 ccgtgtaaat tgccgtgttt ggttcgtcgg aaaccgtaat attggtccca attgtgcggt
      541 acgcctactt tttgccattg tgcatccact tttggatttt gtacaaagct ttcttcctgc
      601 tccggaagct tgtccaaaac aatcgtttcc caagaagagt tcagcggaac ttcttttcga
      661 aattccgctg tcctttcttt gctttgcgac caagcaaagc aactaattaa aaaaccaaat
      721 atgtaaaata cttttttcat attgttaata aaacaaccag tcaatcgcac ctttttcgcc
      781 agcatcaata taaccgacat cgcgagtcgt gatggtttgg ttcgcatcga taaatcctag
      841 aatcaacact tttcctttgc ctaaatccag tttattttcg cccggctgaa aggtgtaagt
      901 atgaatgttt actcttggca attccttcaa attcatcgca cttgccaaaa ttacctcggc
      961 ttgaccgtag gcatttccgg cagcatctgt ctccaaactt ggcggcaaca aaaatctttt
     1021 ttgatccgag ttaaaataac cgaccaccaa ttttacggct ttggtatttt tgaatttcaa
     1081 atgcgttccg ctttcatttt gcgatgtttc atcgaatgta attcctttta ggttttgcag
     1141 ttctggagct atttttgtta gttccgaaat tggcgttccg taaacttttg ttccgttttt
     1201 aacggcataa gttcctttac tctcgctcaa caaagtaact tcggcggttt gccaaggttt
     1261 gccttctttg gttgcaattt ttccgtcttt cgacaatttc aataacgcta aattacgttt
     1321 gaaattagcc atttctcttt cataatgcgg caacaattct gcccaagttt tattgtttcc
     1381 atcgtctccg ccaattggaa ttcggcgttg tgcggtctgc atactgttgg catacaaata
     1441 actatctttc gtcaaattga ccaaagtttg atactcttcg atgctttttt ccaaatgagg
     1501 caaggctttg tccaaatcgg aaatgtcgtt ggaatagctg taacgcaaga ccaacatcgc
     1561 agcttttact ttctccgaaa agaaatcggc gaaggctttg taagcgtgca tatcgttttt
     1621 aaggcgcaga aattcttctt tgtctttcgt cacttttgct tcggctttgt caatggcttc
     1681 aacggccaat cttccgtgtt gggtaatttc gcttatgatt tgaggtggag tttcgccagc
     1741 gtgtgcttcg tgattccatt cttttttggc ataatccaaa agcaattcgg taattggtcc
     1801 acaagattcg tggaatccag gataaacacg ccattttgcc gggtttacca attggctttc
     1861 gaacattccc aacagcaatg tttggcgatt tccttcggta attccaaatt gtcttaatgt
     1921 ttttggggcg atttctccgg cttcttcata ggctttcaaa atattgtttc ccgcttcttc
     1981 atcggttccg tatttcgcag ccaaatcttt gtcccagaat ttaacctctt catttcggtc
     2041 tcttttactg tcccaagcat atctcgacca ggctttgtac caaatccaat cccgatccat
     2101 ttcgagtaaa cgttcacctg ttttggtttt atctgccgaa tatggccaat cccaataaga
     2161 tgcctgcgga tacaaatgca gcgcattggc accgtgaatg ttgtgcattg ccttaaccgt
     2221 tttttggata aaatccggag aaccatatcg gaaaggctcc aagttggcca aaatatgcac
     2281 gttttctatg tgaatggatt tcaaagcact tagctgtctg tgcgtttctc cccaaggacc
     2341 accgggcgca taagtcgtca aagattctcc cgtgtatttg ctctccgtgt acaagttttt
     2401 atacaacgga agcgatttct ccataaccaa tggcgcatcg gtatcgtggg aacgcaaaat
     2461 aattggtggc tcatccgttc ttcccaatgc ttttaaacca tcttgtactc ccggaataat
     2521 ggtttttgta aaccattcga catcatcatc aaccgtgttg atggcttctc ccaaacaaac
     2581 caacaatccc acattggggt atttttcgat aaaagccgca attgattttc tggtataatc
     2641 agaaagcaaa ggcgtaattg atcgtgaacg atcttgcgtt ttgatgttgt aatgttccgc
     2701 aaaaggcttg gaaacaatga tattgtaaaa catttgaatt acccaaattc ctcgtttgtt
     2761 ggcttctacg gtaagatatt tatagatttc ttcgttcttt ttgaaatctt cttcgcttac
     2821 ttccaaagca aatggataat cttttagttt caccaaggag gcaaaagggt gtccgttcca
     2881 caaatacaag gaattcattc gattatcgac catcatatcc aagtactttg tccacagttt
     2941 tttgtcgtaa aaccaaggaa aagtttcggg tgtgtatgga tattcgtaaa cgtcacgacc
     3001 gggaagatag gctgtttttt gcattccaat gcaagaacct ctcaacacca tttcnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgggaa gataggctgt tttttgcatt
     3181 ccaatgcaag aacctctcaa caccatttcg gggctgtcgt caatattgat ttctgaggga
     3241 atttcgccag aggtcttgat gcgatcaact aattccattg tgccatacaa agctcccgtt
     3301 gcatcggttc cttcaatgta aatgatgttt ttttgcgtac aaatttgaaa accttccttt
     3361 ttggttaatt ggctgtcgtc gattttagcg ttttttgaat tttgtttcca aaaatcggtt
     3421 cctttctcgc caataacgat tactttgtct ttcgattttt tgggagcttt atccgtaaaa
     3481 actaccttga atccttttgt ggttgctatt tctgataatt tttcagctcc gaaggttgct
     3541 ctaggagcat tggcatcacg aacaatggtg atgttttgcg ctgatgaaac agacacgtaa
     3601 aaacataaaa aaagtaaact taaatatttc atttgtattg ttattttttg accacaaatt
     3661 gcacaaatta gcacaaatta taatcttgat tttttcgcca cagattacac agatttccac
     3721 agatttaaaa attgctttta acctttaaat ctgtggttaa atatttttaa ttcttgaaaa
     3781 tgctttttaa atattccaca ttggctccat aattggcttt cacatcgtga acttgattta
     3841 catctctgga acgttcaata atcaaccagc caccccattg cattttatcc agcgtctttt
     3901 tgatttccgg caaattcact gctttgttgt tttgtaacca aacaccatcg gtagtcgaag
     3961 cgtgaatttg ggcaatattg tctcttccca atgtttttaa ctctttcgaa atatctcttc
     4021 cgttatccac agcattggca aaattgaatg aaattttgat gtttctattg ccaatttctt
     4081 ccaataattt tttgtcacct gctgcatcca atgacgtttc tacagcgata acaccgccta
     4141 ttttgttgac ttgttcgcca gcccatctta atctttcgat aactttagca cgcaattcag
     4201 gatgtttggc taaatcacat tgtgttccta atggcaaata agcaatttga acattcgtgt
     4261 ttttcatcgt ttcaatacaa tcgtttacca tcttttcaac agtttcacgc tctgcaaacg
     4321 attgggcata aaaaccagac attgcaatag aactaatttc aactccagtt tctttggatt
     4381 tgtccaagaa tttttgtctt tccactggat ctcccaattt actatcgaaa gtttctctgt
     4441 tccctaaacc acccatatcc aattcgattc catcagaatt aatgcttttt gccaattcga
     4501 aagcacctaa tttttgtctt tttagaatca tccaatcaca aactgcaact tggtatattt
     4561 tttttgttgg ccccggccaa actccattaa caataggaaa tgaaactaat tttgacggat
     4621 cgatttttac atatttaatg cgctgtcttc tccaagtata aacacaatgc aacattccat
     4681 cagaaccttg aattatggct ggataagaat actgaccaat tcttgaatct tccaaaacca
     4741 aggaagcatc ccaattgatt ccatcttttg aaacggcaat atttagtggc gttcgaaccc
     4801 cttttgggtc atctccataa ggcaaaacgt ggttgtaaat caaggcgtgt tccccgtttt
     4861 tcatcgttac cgcatcggtt cctgaattgt tgtttggcag attcaaaagc tctaaagccg
     4921 accaagtttt cccgttatca ttcgagaaag tgctaaacaa atgtcggttt cttgtacgtc
     4981 caatggcttg taattttcct tttttatgaa aaagaatact cggttggatg gcactaatag
     5041 cgttaattgt atcttgtttt ttaccgatag tatcgcccat aatccaagtt tttccaaaat
     5101 cgggagtcga ttccatacgc aaatgccaac ctccttttcc ttcgatgctt gaaggaatta
     5161 acaaagtacc attggctaac aaaaccggtt tgtttttgat aggccctaaa aatccttctg
     5221 gcaatttttc gggtttcgac caagtttcgc ctccatcaga agagcgcaat aacattcccc
     5281 accaagttga tggacttggg ccaattttgt aaaaaagcaa caaatcgcct tttggaattt
     5341 ggtacaaaac aggattccaa g
//
LOCUS       DAOSNN010000186         5357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15410_length_5357_cov_4.133346, whole genome shotgun sequence.
ACCESSION   DAOSNN010000186 DAOSNN010000000
VERSION     DAOSNN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15410_length_5357_cov_4.133346"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLQ70_09390"
     CDS             complement(<1..634)
                     /locus_tag="PLQ70_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQE34866.1"
                     /translation="MKIIAVIGSGTMGNGIAHTFAQSGFTVKLIDVSEKSLDKGMATI
                     FANLDRMVAKGTITEEDKFKTINNIITYTDIKDGVVGVDLVVEAATENVELKLNIFKQ
                     LNEVCSHNTIFATNTSSISITQIGAIVSHPERVIGMHFMNPVPIMKLVEIIRGYNTSD
                     EVTKNIMTLSEKLGKTPVEVNDYPGFVANRILMPMINEAIETLYNKVAGVY"
     gene            complement(648..1643)
                     /locus_tag="PLQ70_09395"
     CDS             complement(648..1643)
                     /locus_tag="PLQ70_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014938122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HQE34867.1"
                     /translation="MNYIGSKQKLSSFIQTTVNSVVGDDLSQKTFCDLFAGTGIVGRT
                     FKPNVKKIISNDLEYYSYVLNKNYIGNHEAIDFNSIIEELNQLEGIDGFIFHEYSENG
                     NQNRLYFSEHNGKKIDAIRQQIEHWKTKKSIDTKTYYFLLASLLESADKVANTASVYG
                     AYLKHIKKSAQKNLVLEPAIFSLNDNEHEVFNEDSNLLIQKIEGDILYLDPPYNARQY
                     GSNYHLLNTIAKYDTFIPKGKTGLRDYNRSDYCSKTNVRKSFEELIKNANFGSIFLSY
                     NNEGLMSENEIRTIMSKYGKYDLMTTNYQRFKADKTENRNHTASNTTEFLHILVK"
     gene            complement(1650..2261)
                     /locus_tag="PLQ70_09400"
     CDS             complement(1650..2261)
                     /locus_tag="PLQ70_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34868.1"
                     /translation="MDLVRVETELKKRLIHPYKWGRKQSDDWDAKTSFIYSTYTFEKL
                     LEKTALFDEKARDYALNRWYNFWSAEAVENLFSLHEKVTPNKNKFDKLIDFTINTTCF
                     DHKTTVFPKGFNQTVAYAKSNPTELIQWLYLNQSQESRKHLKNRLFVVLVEKNNEHWK
                     LKAEINLLKTAIDSYVEKFNCDSLIQINLENNAILSDLIWVEN"
     gene            complement(2320..3285)
                     /locus_tag="PLQ70_09405"
     CDS             complement(2320..3285)
                     /locus_tag="PLQ70_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQE34869.1"
                     /translation="MLKVGVLGAGHLGKIHLRLLQQSEKYELVGFYDENQENGAKVAN
                     EFGYKQFSTIATLIHAVDVIDIVTPTLSHFKCAKVAIKSGKHVFIEKPISNTVAEAEE
                     IIALAKEYNVKGQVGHVERFNPAFKATKEMIEKPMFIETHRLAEFNPRGTDVPVVLDL
                     MIHDIDAILSVVNSPVKDVHASGVSVISETPDIANARIEFENGCVANLTASRISLKNM
                     RKSRFFQKDAYISVDFLEKKCEVVKMKDAPEIPGDFDIILQNAEGVKKQIYFSNPDVE
                     QNNAILDELESFADAINNDTTPIVTLEQATEALRVAYQIIDCFKK"
     gene            3417..4058
                     /locus_tag="PLQ70_09410"
     CDS             3417..4058
                     /locus_tag="PLQ70_09410"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQE34870.1"
                     /translation="MKDTAKHQGLRNQLVSLLKEKGITDKAVLEAINKIPRHLFLNSS
                     FSDYAYQDKAFPIGAGQTISQPYTVAFQSQLLEVKKDDKILEIGTGSGYQTAVLCLMG
                     AKVYSVERQKELFKQTSALLPKLGVRPKHLSFGDGYKGLPNHAPFDSIIVTAGAPIIP
                     QALMAQLKIGGRIVIPVGEDTQIMTLLIRKNETQFEKHEFGDFKFVPLLENKK"
     gene            4307..>5357
                     /locus_tag="PLQ70_09415"
     CDS             4307..>5357
                     /locus_tag="PLQ70_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34871.1"
                     /translation="MFLDAFIDEESGKIDFDDTLIRNLEKLPNSENYIAPFINRNMVL
                     ANISNGLCNDLTYKKIDFLASSPKFGKLPIVIYRKAILDRIRMRYDSSLKLFDELGAI
                     TQWQFCGVFENLNSSGLDIEYEPELYAKNDKLFDANSNGKIGWYNPKEALAEGYHFFA
                     NEGEYGNGIIYAQTFIQANEKKDYVLSFGANSGLKIFLNDKEIYVNQDVKRTNLDSYN
                     IKIALKNGNNRLLFKLEMQNGGDYFSAQIKNLDTTIARDLKFLNTFQPYEVSIFENSN
                     AEEIKLNYEQYFDELVKKNPDNLLYRLFQFTAYESNFKKNEAFESIEGLDKKYPNSSF
                     IANYFLRYYDLLGDEA"
BASE COUNT         1755 a          829 c          885 g         1888 t
ORIGIN      
        1 cataaacgcc agccactttg ttgtataaag tttcgatggc ttcgtttatc attggcatca
       61 aaattctatt ggctacaaaa cccggataat cgttaacttc aacaggagtt ttacctagtt
      121 tttcggacaa cgtcataatg tttttggtta cctcgtcgct cgtattataa cctcgaatga
      181 tttcgaccaa tttcattatc ggcacaggat tcataaagtg cataccgata acccgttccg
      241 gatgagacac aatagcacca atttgcgtaa tggaaatgga agaagtattg gttgcaaaaa
      301 tcgtattgtg cgaacaaact tcgtttaatt gtttgaaaat gttgagtttt aattccacgt
      361 tttcagttgc agcttctaca accaaatcta cgccaacaac accgtctttg atgtcggtat
      421 aagtgataat attgttgatg gttttgaatt tgtcttcttc ggtaatcgtt cctttggcaa
      481 ccattcgatc caaattggca aaaatagtcg ccattccttt atccaaagat ttttcggaaa
      541 cgtctattag ttttacggta aaaccacttt gtgcaaaagt atgtgcaatt ccgttgccca
      601 ttgttcctga tccgatgacg gctattattt tcattttttt tctctattta ttttactaaa
      661 atatgtaaaa attcagttgt attacttgcg gtatgatttc tattttcggt tttgtcggct
      721 ttaaaacgtt gataatttgt ggtcatcaaa tcgtattttc cgtatttgga cataatcgtt
      781 ctaatctcgt tttccgacat taaaccttcg ttgttatagc ttagaaatat agaaccgaaa
      841 ttggcatttt ttattaactc ttcaaaggat tttctaacat ttgttttgct gcaataatcc
      901 gaacgattat agtctctaag acctgttttt cctttcggaa taaaagtgtc gtattttgct
      961 attgtgttta ataaatgata attagaacca tattgtcgag cattatacgg tggatccaaa
     1021 tataaaatat cgccttcaat tttttggatt aacagattgc tatcttcatt aaaaacttcg
     1081 tgttcgttgt cgttcaaact aaaaatagca ggttctaaaa ctaggttttt ttgagccgat
     1141 ttcttgatat gctttaaata agcaccataa accgaagctg tattcgccac tttatcagca
     1201 ctttctaata aggaagccaa aagaaaataa taggttttgg tatcgatgct ttttttagtt
     1261 ttccaatgct ctatttgctg ccgaatcgca tctatttttt ttccgttgtg ttcagagaaa
     1321 tacaacctgt tttgatttcc gttttcggaa tattcgtgaa atataaaacc gtcaattcct
     1381 tcaagttgat tgagttcttc aattatagaa ttgaaatcga ttgcctcgtg gtttccaata
     1441 taatttttgt tcaaaacata gctgtaatat tccaaatcat tgctaatgat tttttttaca
     1501 ttgggtttaa aagttctgcc tacaattcca gttccagcaa ataaatcgca aaaggttttt
     1561 tgagacaaat catctccaac caccgaattt actgtggttt gaataaagga agataacttt
     1621 tgtttagagc cgatgtagtt catataaatt taattttcaa cccaaatcag gtcagataaa
     1681 atagcgttat tttcaagatt gatttgaatt aacgaatcgc aattaaattt ttcgacataa
     1741 gaatcaattg cagtttttaa caaatttatt tcggctttta atttccaatg ttcgttgttt
     1801 ttttcgacca aaactacgaa caatctgttt ttcaaatgct ttctactttc ctgactttga
     1861 tttaaataca accattgaat taattcggtg ggattacttt ttgcatacgc taccgtttga
     1921 ttaaaacctt ttggaaaaac agtagtttta tggtcaaaac aagtagtgtt tatagtaaaa
     1981 tcaattagtt tgtcgaattt atttttattg ggcgttactt tttcgtgcaa ggaaaatagg
     2041 ttttcaaccg cttcggccga ccaaaaatta taccatctat tcaaggcata atcacgagct
     2101 ttttcatcaa acaaagctgt tttttctaac aatttttcaa aagtataagt cgaataaata
     2161 aaactggttt ttgcgtccca atcgtcagat tgttttcggc cccatttgta gggatgaatt
     2221 aatcgcttct ttaattcagt ttcaactctt actaaatcca tagattacat tggttttcga
     2281 atttaaattg aaaaatgaat tttgagatat tcagattttt tacttcttaa aacaatcaat
     2341 aatttgatat gccactctca aagcttctgt agcttgttcc aaagttacaa taggagtagt
     2401 gtcattattg atggcatcgg caaaagattc caattcgtcg agaatggcgt tgttttgctc
     2461 cacatcggga ttcgaaaagt agatttgttt tttgacccct tcggcatttt gtagaattat
     2521 atcaaaatcg cctggaattt ctggtgcatc tttcattttg acaacctcgc attttttctc
     2581 caaaaagtca accgagatgt aagcatcttt ctggaaaaaa cgggatttgc gcatattttt
     2641 aagggaaatt ctactggcag ttaaattggc aacacagcca ttttcgaact caattcgagc
     2701 attggcaata tcaggagttt cactaattac ggaaactccg ctggcgtgaa cgtcttttac
     2761 tggtgaattt acaacgctta aaattgcatc aatatcgtga atcattaaat ccaaaaccac
     2821 tggaacatca gtcccacgcg gattgaattc ggccaaacga tgggtttcaa taaacatcgg
     2881 tttttcaatc atttctttgg ttgctttgaa agcaggatta aatctttcga cgtgacctac
     2941 ttgacctttt acgttatatt ccttggctaa agcaataatt tcttcggctt cggcaaccgt
     3001 atttgaaatg ggtttttcta taaagacatg tttacccgat ttgatagcca ctttagcaca
     3061 tttgaagtga gaaagtgtag gtgttacaat atcgataaca tcaacagcgt gaataagagt
     3121 agcaattgtg gagaattgtt tatagccaaa ttcgttggct acttttgctc cgttttcttg
     3181 attttcgtca taaaaaccaa ctaattcata tttttcagat tgttgtaata atcttaaatg
     3241 tatttttcca agatgcccag cacctaaaac cccaactttt aacataaaaa tgatttttaa
     3301 caaatgtata atttatttgt ttattgtttg aattttgggt ttaaagtttt tggaaaatct
     3361 tacttgattt ttgagttttt taagtttaat tttaccgaaa caattaactc caaattttga
     3421 aagatactgc caaacatcaa gggcttcgca atcaattagt aagccttttg aaagaaaaag
     3481 gaattacaga taaagcggtt ttggaagcca taaataaaat tccgagacat ctgtttttaa
     3541 attctagctt ttcagattat gcttatcaag ataaagcatt tccgatagga gcagggcaaa
     3601 ctatttcgca accttatact gtggcttttc aaagtcagtt gttagaagta aaaaaagacg
     3661 ataaaatttt ggaaattgga actggttcag gttatcaaac tgctgttttg tgtttgatgg
     3721 gagccaaggt ttacagtgtc gaaagacaaa aagaattatt caaacaaaca tcggcattat
     3781 tacccaaatt aggtgttaga cctaaacatc tttcttttgg tgatggttac aaaggtttgc
     3841 ctaatcacgc tccttttgat agtataattg taacggctgg tgcgccaata attccacaag
     3901 ctttgatggc tcaattaaaa ataggaggaa ggattgtgat tccggtgggg gaagacacac
     3961 aaattatgac acttctgatc cggaaaaatg aaactcaatt tgagaaacat gaatttggtg
     4021 attttaaatt tgtgcctttg ttagaaaata aaaaataaaa gtaaaactta caaataaaat
     4081 atctttttac attaaaaatt gacagaaatt attacattta ctcattaaat ttatacttta
     4141 cttaatgaga aaaacatttt atatagtttt actactatgt gccaacttta tttactctca
     4201 agattacacc aatgttatta attaataaat taataataaa agagaagaag ctagaaagtt
     4261 atttgacaaa caatttgata aaacgaaaac aacaaatatt gatttattgt ttttagatgc
     4321 ttttattgat gaagaatcgg gtaaaataga ttttgatgat acattaatcc gaaatttaga
     4381 aaaactccct aattcagaaa attatattgc tccttttatt aatagaaata tggttcttgc
     4441 taatatttct aatggtcttt gcaacgattt gacctataaa aaaattgatt tcttggcatc
     4501 ttcgccaaag tttggaaaat tgcctattgt aatatatcgc aaagctattt tagatcgaat
     4561 aagaatgaga tatgatagtt cgttaaaatt atttgatgaa cttggagcta taactcaatg
     4621 gcagttttgt ggagtattcg aaaacttaaa cagtagcggt ttagatattg aatatgaacc
     4681 agaattgtat gctaaaaatg acaaactttt tgatgcaaat agtaatggaa aaatagggtg
     4741 gtataaccct aaagaagctt tggcagaagg gtatcatttt tttgcaaatg aaggcgaata
     4801 tggaaacgga ataatttatg cacaaacctt tattcaagca aatgaaaaaa aagattatgt
     4861 cttgagtttt ggagcaaatt ctggtctaaa aatatttttg aatgataaag aaatatatgt
     4921 aaatcaggat gttaagcgaa ctaatttaga ctcatataat ataaaaatcg cattgaaaaa
     4981 tgggaacaat agattattgt ttaaattaga aatgcaaaat ggaggggatt atttttcggc
     5041 tcaaattaag aatttagaca ctacaattgc tagggattta aagtttttga acacttttca
     5101 gccctatgaa gtatcaatat tcgagaatag taatgctgaa gaaataaaac ttaattatga
     5161 acaatatttt gatgagttag tcaaaaaaaa tcctgataat ttattgtatc gtttatttca
     5221 gtttacagca tacgaatcaa attttaaaaa aaacgaagca tttgaatcaa tagaaggatt
     5281 agataagaaa tatccaaata gctcgtttat agcgaattat tttttgagat attatgattt
     5341 actaggagat gaagctg
//
LOCUS       DAOSNN010000187         5354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15421_length_5354_cov_4.477449, whole genome shotgun sequence.
ACCESSION   DAOSNN010000187 DAOSNN010000000
VERSION     DAOSNN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15421_length_5354_cov_4.477449"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLQ70_09420"
     CDS             <1..292
                     /locus_tag="PLQ70_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQE34872.1"
                     /translation="GDLKEASEHMISISQKTVKERLAETLIYLDETFGKNPDGSLRIQ
                     LSREEMAGMIGTATESCIRLLSDFNKIGLISLIGKKIVVKDTAKLKRIADIV"
     gene            complement(352..1125)
                     /gene="mazG"
                     /locus_tag="PLQ70_09425"
     CDS             complement(352..1125)
                     /gene="mazG"
                     /locus_tag="PLQ70_09425"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQE34873.1"
                     /translation="MNSKQNQLQAFERLLNIMDDLREKCPWDKKQTLQTLRHLTIEET
                     YELGDAILDNDLNEVKKELGDLLLHIVFYAKIGSETNDFDIADVCNEICEKLIHRHPH
                     IYSDVVVKDEEEVKQNWEKLKLKEGKKSVLEGVPRSLPALVKASRIQDKVKGVGFDWE
                     EPHQVWDKVQEELAELQVEVEAGNQDKMEAEFGDVLFSMINYARFLNINPEDALERTN
                     KKFIKRFQYLESKAGELGKPLMDMTLAEMDVFWNEAKKL"
     gene            complement(1217..1672)
                     /locus_tag="PLQ70_09430"
     CDS             complement(1217..1672)
                     /locus_tag="PLQ70_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HQE34874.1"
                     /translation="MNLEKILSISGKPGLYALKVQTRTGFLAESLVDGKKISVSLKVN
                     VSLLSEISIYTVNEEKPLTEVMRNIAIKENEGPAISHKEDNATLIAYFSEILPEYDSE
                     RVYPSDIKKVLNWYNLLQSKGLVSKEEPKIENAEEVLEKVVENVTADKE"
     gene            complement(1724..2311)
                     /gene="def"
                     /locus_tag="PLQ70_09435"
     CDS             complement(1724..2311)
                     /gene="def"
                     /locus_tag="PLQ70_09435"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQE34875.1"
                     /translation="MILPIVGYGDPVLRKVGEEITPEYPNLKETIANMYETMYNAYGV
                     GLAAQQVGLNIRLFVIDTTPFGEDEDLPLEVQSQLKGFKRTFINAKMLKEEGELWSFN
                     EGCLSIPDVREEVYRNERITIEYCDENFNVKTEVFDGLIARVIQHEYDHIEGILFTDL
                     ISTLKRQLNKKKLQNIMDGKARPDYRMKFCNKKGR"
     gene            2503..4782
                     /locus_tag="PLQ70_09440"
     CDS             2503..4782
                     /locus_tag="PLQ70_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HQE34876.1"
                     /translation="MIPKIKDFTIGTNLHNAIKVVLSAIIPAFLFSYLGNLEVGLTMA
                     IGALFTFPTDTPSNLKHKINGLLVTVFILAGVNLLINATYPYPFLFYPIFAILVFILS
                     MLAVYGQRATMVSFAALMIISISFAHIHKGLDMLEHSGFLLAGGLFYLLISILFYYIN
                     PHRYTELQIVECIKLTSKYLKLRGDLWQLDSDRKEITRKQLVLQVELNDIHENIREVL
                     VRNRTDYGSSHQNRKLLLAFISLVEIMELAVSTSFDHNKLHQKFDAHPKVLMTYQSLA
                     YNLAKNLKSLSKKIKKRKQYIQKNNLVENLFAFEFAIADYEKTLGEAEASEGVHMLSN
                     MLHYAEKQVEKIKTLERAYTTTVKLKDLKGRDKDLEKLSISQYYPLNTLVENFSFSSL
                     EFRHSLRITTTLLIGLIIGKVLPFENVYWILLTIVVIMRPGYGLTKERTLNRFIGTLI
                     GGVLGFAVLTFEPSTPILATLTILFLILGLTFNPSNYKIGTSFITLHVIFIFAILNPS
                     DGNIILYRVLDTFVGAILSILANHFLWPYWESLNTNENIKNSIEANKNYLKQISILYN
                     NKQGINQEYRLARNQAFIEIGNMMASFQRMLQEPKSRQDKLQQVYKFTVINNALLSSA
                     ASLGTYTQSHKTTKASEAFNVVFDKIIKNLDAAISLLKEENQPNEIDAPIDDTFNNSF
                     IELKNIREKELRQGDVINSQEFKLKMQEAQLVIEQLVWLTNLSENILKTTKNLLKAEK
                     QPENNTLKTFFGSNSPEAL"
     gene            complement(4742..5158)
                     /gene="ruvX"
                     /locus_tag="PLQ70_09445"
     CDS             complement(4742..5158)
                     /gene="ruvX"
                     /locus_tag="PLQ70_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQE34877.1"
                     /translation="MPRILAIDYGQKRTGIAVTDELQIIASGLTTIPSSEAIPFLKDY
                     FSKEKVEAVLIGEPKQMNGEPSQSASIIKGFVTHFTNHFPEMKVIRVDERFTSKMAFQ
                     SMIDSGMKKKQRQNKALIDEISATIMLQDYLTRKMF"
BASE COUNT         1710 a          950 c          819 g         1875 t
ORIGIN      
        1 tggggatttg aaagaggcca gtgaacatat gatttctatt tctcaaaaaa cagtcaaaga
       61 acgattagca gaaactctta tttatctcga tgaaactttt ggtaaaaacc cagacggatc
      121 gctacgcatt caattgtctc gtgaagaaat ggctggaatg attggaacgg ccaccgaaag
      181 ttgcattcga ttgttatctg attttaataa aattggatta attagtttga ttggtaaaaa
      241 aatagtggtt aaggatactg ctaaactgaa gcgaattgcc gatatcgttt aaaaatattt
      301 tagattttag actgtagatt tttgaatact gatttgggaa aaatcaaaaa actacagttt
      361 ctttgcttca ttccaaaaaa catccatttc ggccaaagtc atatccatca agggtttgcc
      421 taattctcct gctttgcttt ccagatattg aaatcttttg atgaattttt tattggttcg
      481 ctccaaagca tcttcgggat ttatgtttaa aaatcgtgca taatttatca ttgagaatag
      541 gacatcgcca aattcggctt ccattttgtc ctgatttcct gcttcgacct ctacctgaag
      601 ctcggctaat tcctcttgga ctttatccca aacctgatgc ggttcttccc aatcgaagcc
      661 tactcctttt actttgtctt gaattcggct ggcttttact aaggctggca aacttcttgg
      721 aacgccttca agaactgatt ttttgccttc tttgagtttc agtttttccc agttttgttt
      781 cacttcttct tcatctttta ccacgacatc gctgtaaata tgaggatgac gatgaatgag
      841 tttttcacag atttcattac agacatcggc tatatcaaaa tcattggttt cgctccctat
      901 tttggcataa aaaacgatgt gtaacaacaa atcacccaat tcttttttga cttcgttcaa
      961 atcattatct aaaatggcat cgcccaattc ataggtttct tcgatggtta aatgcctcaa
     1021 cgtttggagt gtttgctttt tatcccaagg acatttttcg cgcaaatcgt ccataatgtt
     1081 caataatcgt tcgaaggctt gaagttgatt ttgtttggaa ttcatggtgt cttaattttt
     1141 gtaaaaataa gaaatcctgt caggaaaaca tcttgacagg attaaaatat aaaattaaag
     1201 tttattgctt tttaccttac tctttatcgg cagttacgtt ttcaacaact ttctctaaaa
     1261 cctcttcagc attctcaatt tttggttctt ctttagaaac taaacctttt gattgaagca
     1321 aattatacca atttaatact tttttaatat ctgaaggata cactctttct gaatcgtatt
     1381 ctggaagaat ttctgaaaaa taagctatca aagtagcgtt gtcttcttta tgcgaaatgg
     1441 ctggaccttc attttctttg atggcgatat tgcgcatcac ttcagttaaa ggtttttctt
     1501 cattgacagt gtaaattgaa atttcagaca ataaactcac gtttactttc aagcttactg
     1561 atattttttt tccatcaact aatgattctg ccaaaaatcc tgttcgagtt tgaactttta
     1621 atgcatataa acctggtttt ccagaaattg ataatatttt ttctaaattc attttttata
     1681 aattatatgt ttttatttat tttttgagaa actgaatact ttcctatctt ccctttttat
     1741 tgcaaaattt cattctataa tcaggtctag ctttgccatc cataatattt tgcaatttct
     1801 ttttatttaa ttgccttttt agtgtcgaaa tcaaatcggt aaacaaaatc ccttcgatat
     1861 gatcgtactc atgttgaatt acacgagcaa taagtccatc aaaaacttca gtttttacat
     1921 tgaaattttc atcgcaatac tcaattgtga ttctttcgtt tctataaacc tcttcgcgaa
     1981 catctggaat actgaggcaa ccttcgttaa aactccataa ttcgccttct tctttcaaca
     2041 ttttggcatt aataaaagtt cttttgaagc cttttaattg gctttgtact tctaatggca
     2101 aatcttcatc ttctccaaaa ggtgttgtat caattacaaa aagacgaatg ttcaaaccta
     2161 cttgttgagc tgcaagccca acgccataag cattatacat cgtttcatac atgtttgcaa
     2221 tagtttcttt taggtttgga tattctggag taatctcctc tcctaccttt cttaaaaccg
     2281 gatcgccata tcctacaatt ggtaaaatca ttaattttgt attagagtat tatctactgc
     2341 aaattctaaa tcgattattt aaaattcagt ccgcaaaagt agtaaaaaaa tatgcattca
     2401 atatatccta tattaataat taaatttact ttagaaatct aaaccctaca taccgatata
     2461 atataccaca attcttacct ttgcaatcaa tctaaactat ttatgatacc aaaaataaag
     2521 gactttacta ttggcactaa tttgcataat gcgatcaaag tagttctttc tgccattata
     2581 cctgcattct tattttccta tctaggcaat ttagaagtgg gtttgacaat ggcaattggt
     2641 gctttattta catttccgac agacacccca agtaacttaa aacataaaat aaatggcttg
     2701 cttgtaacgg tatttatact tgctggagtc aatttattaa tcaacgccac ttatccttat
     2761 ccttttcttt tttaccccat atttgccatt ctagttttca tattatccat gcttgctgtt
     2821 tacggacaac gcgctacgat ggtttctttt gcggcattaa tgataatcag catttcattt
     2881 gcacacatac acaaaggttt ggatatgcta gaacactctg gttttttatt agctggaggt
     2941 ttattttatc ttctaatatc tatccttttt tattatatca atccgcatcg ctataccgaa
     3001 ttacaaattg ttgaatgtat aaaattgaca tcgaagtatt tgaaattaag aggcgattta
     3061 tggcaattgg attcggatag aaaagaaatt acacgaaaac aattggtcct acaggtagaa
     3121 ttgaatgata ttcacgaaaa tattagagaa gttctagttc gaaaccgaac cgattatggc
     3181 tcttcgcatc aaaacagaaa attgcttttg gcctttattt ccttagtaga aataatggaa
     3241 ttggcggtat caacctcttt tgatcataat aaattgcacc aaaaatttga tgctcatcca
     3301 aaggtcttaa tgacttacca aagtctggct tataatttgg ctaaaaattt aaaatcttta
     3361 tccaaaaaaa taaagaagag aaagcaatac attcaaaaaa ataatttggt cgaaaattta
     3421 tttgcttttg aatttgccat agctgattat gaaaaaacac ttggagaagc agaggcttca
     3481 gaaggggttc atatgctttc gaatatgctg cattatgctg aaaaacaagt tgaaaaaata
     3541 aagactttgg agcgtgctta taccacaact gtaaagctaa aagaccttaa aggaagagat
     3601 aaagatttag aaaaattaag catctctcaa tattatccgc taaatacttt ggttgaaaat
     3661 ttcagttttt cttcgcttga atttagacat tcattacgaa tcaccaccac cttattaata
     3721 ggtttaataa tcggcaaagt ccttccgttt gaaaatgtct attggatatt attgaccata
     3781 gtagtaatta tgcgccctgg ttatggattg acaaaagaaa gaaccttaaa ccgttttata
     3841 ggaacgctca tcggaggtgt tttgggtttt gcagttttaa cttttgaacc aagcacacca
     3901 attcttgcca cactaaccat tcttttttta atcttaggac ttacctttaa tccttccaat
     3961 tataaaattg ggacttcctt cattacttta catgtcatct ttatttttgc catattaaat
     4021 ccaagtgatg gcaatattat tctgtatcgt gttttagata cttttgttgg agccatttta
     4081 tcgattttgg ccaatcattt tttgtggccc tattgggaat ccttaaatac aaatgaaaat
     4141 atcaaaaact ctattgaagc caataaaaac tatttgaaac aaatttcaat tctctacaat
     4201 aataaacaag gcattaatca agaatacaga ctagctagaa atcaagcttt tatagaaatt
     4261 ggtaatatga tggcgtcttt tcaacgaatg ttgcaagaac ccaaatcaag acaggataaa
     4321 ttgcagcaag tatataaatt caccgtaata aataatgctt tactttcctc ggctgcttcg
     4381 ctaggaacct atacgcaatc tcataaaacg accaaagctt ctgaggcttt taacgtcgta
     4441 ttcgataaaa taatcaaaaa tctggatgct gctatttccc ttttgaaaga agaaaatcaa
     4501 ccaaacgaaa ttgatgcgcc aattgacgac acctttaata acagctttat cgaattaaaa
     4561 aatattaggg aaaaagaatt aagacaagga gatgttatta atagtcaaga atttaaactc
     4621 aagatgcagg aagctcaact agtgattgag caattggttt ggctaactaa tttgtcagaa
     4681 aacattctta aaaccacaaa aaacttatta aaagctgaaa aacaacccga aaacaatacg
     4741 cttaaaacat ttttcgggtc aaatagtcct gaagcattat agttgccgat atttcgtcaa
     4801 ttagggcttt gttttgacgt tgtttttttt tcattccact atcaatcatc gactggaacg
     4861 ccattttgga agtaaaacgt tcatccaccc tgatgacctt catctctggg aaatgattgg
     4921 taaaatgcgt cacaaatcct ttaatgatgg aagcactttg agaaggttca ccgttcattt
     4981 gtttgggttc gccaataagc acagcttcca ctttttcttt cgaaaaatag tctttcaaaa
     5041 atgggatagc ttcacttgat ggaatagtgg tcaaacccga agcaatgatt tgcaattcat
     5101 cggtaacagc tattccagtt cgtttttgtc cgtaatctat ggcgagtatt ctaggcattt
     5161 attttttatt caaagatagg aaggaataat taattggcat gataattatt attattttgc
     5221 aaagttaaat atgtaacata ttctaacgaa tttgcctttt ggcttttggc taaaaatggt
     5281 atatttgcca aaaatttaac aaaaatgaac gaattacaat ccataataga acaagcttgg
     5341 gaaaaccgtg cttt
//
LOCUS       DAOSNN010000188         5335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15506_length_5335_cov_4.967045, whole genome shotgun sequence.
ACCESSION   DAOSNN010000188 DAOSNN010000000
VERSION     DAOSNN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15506_length_5335_cov_4.967045"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PLQ70_09450"
     CDS             complement(<1..1038)
                     /locus_tag="PLQ70_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HQE34878.1"
                     /translation="MKKIIFAFAFATVLWSCKTASTASATSNKEEIQVNINLNEIKND
                     KVLVTVKSPKIKTDEVTYHIPKTVPGTYSEDNYGKHIDDLKAFDKKGNPLTVKKTDEN
                     SWSIAKAKTLDKITYFVNDTYDTEKGEGFGSGDIFSPSGSNIDAGKNIMLNTHCFVGY
                     FSDYLSVPYKVSISHPTTLWGATSMTDQDPATTNDLFITPRYAELVENPIMYSKPNYT
                     TFTVDNMEIQIAVYSPNGKITAESITPEMKTMMTAQKRFLGSINSTKKYSVLLYLSTM
                     NKDAQGFGALEHPTATTVVFPEMLPKDELTKEMKDVVSHEFFHIVTPLTIHSQEIQNF
                     DYNAPKMSEHLW"
     gene            complement(1155..1433)
                     /locus_tag="PLQ70_09455"
     CDS             complement(1155..1433)
                     /locus_tag="PLQ70_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2805 domain-containing protein"
                     /protein_id="HQE34879.1"
                     /translation="MKKSNRTELNWEQTEKLVTFALEERNPFEIIKKEFGLSEKEVLE
                     IMKKKMPAEKFEMWKKKALASKPKPKPAKIDDFDEDLDGKYYIKNKLD"
     gene            complement(1558..2385)
                     /locus_tag="PLQ70_09460"
     CDS             complement(1558..2385)
                     /locus_tag="PLQ70_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQE34880.1"
                     /translation="MNEIIAYFSTIPSSHRSLILVGGITFFWLIENAFPLFHFKYKKW
                     QHAGINFFLTFTTILINFTLAFLLLKASDWTIATNFGILQWLPTMSLWLHTVIGLLLL
                     DLIGAYLAHLIEHKIKFLWRFHLIHHTDTWIDTTSGNRHHPGESIIRFAFTIIGIVVV
                     GSPMWLVFIYQTISVVSTQFTHANFSFPEKIDTIMSYFLVSPNMHKVHHHYKLPYTDS
                     NYGNIFSIWDRLFGTFMYLKKEEIVYGIDTHSKPDENNQLMNLLKIPFQKLRSPEND"
     gene            complement(2430..3032)
                     /locus_tag="PLQ70_09465"
     CDS             complement(2430..3032)
                     /locus_tag="PLQ70_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HQE34881.1"
                     /translation="MNSLFQSEPIVFNLPDADIIYYPHFFNKEEADAIFEQLTTDIPW
                     QQDEIRVFGKVHPQPRLTALFGNDGKSYSYSNIKMQPLPWNLILQKIKSQIETVIETD
                     FTTVLLNLYRDGKDSNGWHADNEKELGNNPIIASVSFGTERVFQLKHNSIPGLKQNVL
                     LEHGSLLVMKGTTQHFWKHQIPKTSKLIGSRINLTFRIIK"
     gene            complement(3105..3812)
                     /gene="aqpZ"
                     /locus_tag="PLQ70_09470"
     CDS             complement(3105..3812)
                     /gene="aqpZ"
                     /locus_tag="PLQ70_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HQE34882.1"
                     /translation="MRKLLAEFFGTYWLVFGGCGSALFAAGIPDLGIGFVGVSLAFGL
                     TVLTMAYAVGHISGGHFNPAVSFGLWAGGRFSAKELVPYIIAQCAGAIAAAGTLFAIA
                     SGKAGFSIDPSKAGAFASNGFDAFSPDGYSMQSAFIAEFILTMFFLLVILGATDKFAN
                     GKFAGIAIGLALTLIHLISIPITNTSVNPARSLSQAIFVGGEPLSQVWLFWAAPILGA
                     IAAGFIYKNLLQDTSEA"
     gene            complement(3968..4198)
                     /locus_tag="PLQ70_09475"
     CDS             complement(3968..4198)
                     /locus_tag="PLQ70_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HQE34883.1"
                     /translation="MLGITLTEAVGYLASLVLMISFLMKNINTLRIVNSTGCLLFVIY
                     GFMLATSWPIIISNAFILGVNIFYLTKHFRAN"
     gene            complement(4270..>5335)
                     /locus_tag="PLQ70_09480"
     CDS             complement(4270..>5335)
                     /locus_tag="PLQ70_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HQE34884.1"
                     /translation="GEQCIVFVQLNGGNDGLNTFIPFEDPLYYNPRPTIALSKNEVLN
                     SAKGMAFHPALKGFASMQQSGDLSVIQNVGYPNPVRSHFRSQEIWQTAPTHQEYLSDG
                     WLGRYLDLQCKEHQPTAGINIDTIDNLALKGEEPNSITVKDPDRFKTKSKAENDGQLS
                     KNPQLDFVRKVANSVVEGSDDIQKALAKSAPSDVVYPKTGLAKNLEWISKLIKGNLNS
                     KVYYTSLGGFDTHDNQLSIHKNKLTELNDAVFSFYEDLKKSQQLQNVTIVVFSEFGRR
                     VKDNGRGTDHGTAAPMFVIGGNNRGKIIGNNPNLSDLDNGDLKHEIDFRSVYASILKN
                     KLDFDFTKIGIQNKALEGLF"
BASE COUNT         1709 a          906 c          915 g         1805 t
ORIGIN      
        1 ccataaatgt tccgacattt ttggagcatt ataatcaaaa ttttgaattt cttgagaatg
       61 aatggtcaaa ggcgttacga tatgaaaaaa ctcatgcgaa acgacatctt tcatctcttt
      121 tgttaattca tcttttggaa gcatttctgg aaataccacg gttgttgcag ttggatgttc
      181 caaagcacca aatccttgag catccttgtt catagtggac aaatacaata aaacgctata
      241 ttttttggtt gaattgatag accctaagaa acgtttttga gccgtcatca tggttttcat
      301 ttcgggcgtt atgctctctg ctgtaatttt tccgtttgga gaatatacgg caatttgaat
      361 ttccatatta tctactgtga aagtagtata attaggtttt gaatacataa ttgggttttc
      421 gactaattca gcataacgtg gcgttataaa taaatcattt gtagtagctg gatcttgatc
      481 tgtcattgaa gttgctcccc aaagagttgt tgggtgagaa atagaaactt tataaggaac
      541 cgacaaataa tctgaaaaat agccaacaaa acagtgcgtg ttaagcatta tgtttttgcc
      601 tgcatcaatg tttgaaccac taggcgaaaa aatatctcca gaaccaaagc cctctccttt
      661 ttcggtgtca taagtatcgt ttacaaaata ggtaatttta tccaaagttt tagctttagc
      721 tattgaccaa gaattttcat ccgttttttt aacagttaaa gggtttcctt ttttgtcgaa
      781 agcttttaaa tcatcaatat gttttccata attgtcttca gaataggttc ctggaactgt
      841 ttttgggatg tgataagtca cttcatctgt tttgatttta ggtgatttaa cggtaactaa
      901 aaccttgtca ttcttgattt catttagatt aatgttaacc tgaatttctt ctttattaga
      961 agtcgcacta gcagtactag cagttttaca actccataga acagttgcaa aagcaaaagc
     1021 aaatattatt tttttcattt tatgttttta tttgtgatat atgactttga aagtatagat
     1081 atgttacact taaactcagt ttttaaagga taatttgaat aataaaaaaa ctcctgaata
     1141 tcaggagtca aatgttaatc aagtttgttt tttatataat atttaccatc taagtcttcg
     1201 tcaaagtcgt caatttttgc aggttttggt tttggtttac tggctaaagc tttctttttc
     1261 cacatttcaa atttctcagc aggcatcttt tttttcatga tttcaagaac ctctttttca
     1321 gataacccaa attctttttt gatgatttca aaagggtttc tttcctctaa ggcgaacgta
     1381 acaagttttt ctgtttgttc ccaatttaat tctgtgcggt tactcttctt cattaggtga
     1441 aaattaattc aattttatat ttagggttaa tgttaataga tttgttttta aatctttgtc
     1501 caatattaat aaaaaaaata attacttttt atctaaaggt gtattttttt atgattttta
     1561 atcgttttct ggcgaacgga gtttttgaaa tggtattttt aataaattca tcaattgatt
     1621 attttcgtct ggctttgaat gtgtgtctat tccatatact atttcttctt ttttgagata
     1681 cataaatgtg ccaaaaagcc gatcccaaat cgaaaaaata ttaccgtaat tgctatccgt
     1741 ataaggcaat ttatagtgat gatgcacctt gtgcatattg ggtgaaacca aaaaataact
     1801 cattattgta tctatttttt cgggaaatga aaaattagca tgagtgaact gtgtagaaac
     1861 aaccgaaata gtttggtaga taaaaactaa ccacattggg cttccaacaa ctactattcc
     1921 tatgattgta aatgcaaaac ggatgatact ttcgccagga tgatgcctat ttccagatgt
     1981 tgtatctatc caagtatccg tatgatgaat taagtgaaaa cgccatagaa atttgatttt
     2041 atgctcaatt aaatgagcca aataagctcc aattaaatcc agtagcaata acccaattac
     2101 tgtatgaagc cataaagaca ttgttggtaa ccattgtaaa ataccaaaat tggttgcaat
     2161 tgtccaatca gaagctttta gtaaaagaaa agctaaagta aaattaataa gaatggtagt
     2221 aaaggtcaga aaaaaattta ttccagcatg ttgccatttt ttatacttga aatgaaagag
     2281 cggaaaagca ttttctatta accaaaaaaa agtaattcct cccacaagaa tcaaacttct
     2341 atgtgaggat gggattgtag aaaagtaagc aataatctcg ttcataatca gtattttatt
     2401 ccaaatctaa taatttttgg ataatatgat tatttaatga ttcgaaatgt tagatttatt
     2461 ctggatccaa tgagttttga cgttttcgga atttgatgtt tccagaaatg ttgtgtggtt
     2521 cctttcatca ccaataagct gccgtgttca agcaaaacat tttgtttgag tcccggaatt
     2581 gaattgtgtt tgagttgaaa aacacgttca gtcccaaaac taaccgaagc aattatagga
     2641 ttgttgccca attctttttc gttgtcggcg tgccaaccgt tgctgtcttt tccgtctcga
     2701 tagagattga gtagcactgt ggtaaaatcg gtttcgataa cagtttcaat ctgtgatttt
     2761 atcttttgca gaattagatt ccacggcaga ggttgcattt tgatattcga ataggagtac
     2821 gactttccgt catttccaaa caaagctgtt aaacgaggtt gcggatgtac tttcccaaaa
     2881 actcgaattt cgtcttgttg ccaaggaatg tcagttgtta attgttcaaa aattgcatca
     2941 gcttcttcct tgttaaaaaa atgaggataa taaataatgt ctgcgtctgg gagattaaaa
     3001 acaatgggtt ctgattggaa aagtgagttc ataatgcaaa aataaagaga aaagcaaaga
     3061 ttttcatctt tgcttttctc tttaaaatat attttgaaaa aaaattaagc ttctgaagtg
     3121 tcctgtaaaa gatttttata aataaatcca gctgctattg ctcctaaaat tggtgctgcc
     3181 caaaacaacc aaacttgtga caatggttcg ccacctacaa atattgcttg agacaaagaa
     3241 cgagcaggat ttacagatgt attggtaatt ggaatactga ttaagtgaat taaggttaaa
     3301 gctaatccaa ttgcgattcc agcaaatttt ccgttggcaa atttatcagt tgctccaaga
     3361 attactaaca agaaaaacat agtcaaaatg aattcagcta taaatgccga ttgcatagaa
     3421 taaccatcag gagaaaaagc atcaaaacca ttagaggcaa aagcacctgc ttttgaagga
     3481 tctattgaaa aacctgcttt tcctgatgca attgcaaaca aagttccagc agcggcaatc
     3541 gctccagcgc attgtgcaat gatataagga actaattctt tggcagaaaa tcgacctcca
     3601 gcccataatc caaaagaaac tgcaggatta aaatgtccgc cagaaatatg acccacagca
     3661 taagccattg tcaaaacggt taaaccaaat gcaagagaaa cccccacgaa tccaattcct
     3721 aaatcgggaa ttccagcagc aaaaagtgcg ctaccgcaac ctccaaaaac caaccaatac
     3781 gtgccaaaaa attcagccaa taattttctc ataatgtaaa atttagattg ttaatgcata
     3841 cgaagttaag gtagatgcat tttaaaatca aaattttttg ttaaattttt gtaaatcgac
     3901 actgtttttg caattaatat tttcgatttt ttaaaattta accgtattat aacgttaaga
     3961 atggtgttta attggctcga aaatgcttgg tcaaataaaa aatattgact cccaagatga
     4021 aagcattgga aatgatgatc ggccaagagg ttgccaacat aaaaccataa atcacaaata
     4081 ataagcatcc agtagagttg actattcgta atgtgttgat gtttttcatt agaaaagaaa
     4141 tcatcaatac taatgaggcc aaatatccta ccgcttctgt taatgtaata cctaacattt
     4201 ttttattttt aaattgaagt ataaaaataa gggatagtta tggtttgaag atagaaattt
     4261 tagacttttt taaaacaatc cttctaaagc cttgttttgt attccaattt tggtgaaatc
     4321 aaaatccagt ttgtttttca aaatcgaggc atacacactt ctaaaatcaa tttcgtgctt
     4381 caaatcgcca ttgtccaaat ccgaaagatt gggattgttt ccgattattt tgccgcggtt
     4441 gtttcctcca atgacaaaca ttggtgcagc ggttccgtga tcggtgcctc gtccattgtc
     4501 tttcacgcga cgaccaaatt ccgagaaaac aacaatcgta acgttttgta attgttgcga
     4561 ttttttcaaa tcttcataaa aactaaaaac ggcatcattg agttctgtca atttattttt
     4621 atgaatggaa agctggttgt cgtgcgtgtc aaaaccgccc aaagaggtat aatacacttt
     4681 cgaattcagg tttcctttta tcaattttga aatccattcg agatttttgg ctaagcctgt
     4741 tttgggataa acgacatccg aaggtgctga ttttgccaat gctttttgaa tgtcatccga
     4801 gccttccact accgaattgg ctacttttcg aacaaaatcc aattgtggat tcttggataa
     4861 ttgaccgtca ttttcggcct tgcttttggt tttgaatcgg tcgggatctt tcaccgtaat
     4921 cgagttgggt tcttcccctt tcaaagctaa attgtcaatc gtgtctatat ttattccagc
     4981 cgtaggttgg tgttctttgc attgcaaatc aagataacgt cccaaccagc catcacttaa
     5041 atattcctga tgggttggtg cagtttgcca aatttcctga ctcctgaaat gagaacgaac
     5101 cggattcgga tagcccacat tttgtatcac tgacaaatcg ccactttgtt gcatactggc
     5161 aaatcctttc aatgcgggat gaaacgccat accttttgct gaatttagaa cttcattttt
     5221 tgaaagagca atcgtgggac gcgggttata ataaagaggg tcttcaaacg gtataaaagt
     5281 attcaatcca tcgtttccac cattcaattg aacaaaaacg atgcattgtt cgcct
//
LOCUS       DAOSNN010000189         5333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15517_length_5333_cov_5.003031, whole genome shotgun sequence.
ACCESSION   DAOSNN010000189 DAOSNN010000000
VERSION     DAOSNN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15517_length_5333_cov_5.003031"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1121
                     /locus_tag="PLQ70_09485"
     CDS             108..1121
                     /locus_tag="PLQ70_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HQE34885.1"
                     /translation="MKYIVCEKPHEFKLKEKPMPEPKEGEVLLKIKRIGVCGTDIHAY
                     GGTQPYFEYPRVLGHELAAEYIKGNAKGFEPGDKVTLIPYFNCGECVACRNGLTNCCA
                     NIKVFGVHIDGGMAEYITVPEQYLLHSQGLDYDELALVEPLAIAAHGVRRAAVEAKDT
                     VLVMGAGPIGIGLIQFAKIAGAKVIVLDINDFRLDFCKKELQADAVINPMNEDVTERL
                     KQITNGDMVNVVIDATGNRNVMNGAFNYVAHGGRFVLVGLQKGELSFSHPEFHKRETT
                     LMSSRNATIEDFEFVMKCLKSGKIDPKKYITHRANFTDMIDVFDQWIDPKNNTIKAII
                     ELN"
     gene            1139..1939
                     /locus_tag="PLQ70_09490"
     CDS             1139..1939
                     /locus_tag="PLQ70_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE34886.1"
                     /translation="MDLHLKGKIVVVSGSAGKEGSIGETIVKRLADEGAIPALVDRNS
                     RGFGYAEELKKRGIDAIFVQTDVTNPEQMENAVNKIVEKYGRIDAVINNVGVNDGAGL
                     DSSYEEFMNSLKLNVVSYFLLTKFALPYLKASKGNILNIGSKVALTGQGGTSGYAAAK
                     GGVLGLTREWAVDLIQFGIRSNAIIIAESFTPAYDEWLKTLPDGEAVLQKINKSIPFE
                     ARMTKAEEIADTALFIVSDRSSHTTGQFVCVDGGYIHLDRALINDVSL"
     gene            1945..2781
                     /locus_tag="PLQ70_09495"
     CDS             1945..2781
                     /locus_tag="PLQ70_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQE34887.1"
                     /translation="MKNRIDSHQHFWKFDPVRDSWIDDSMLKIQKDFLPEDLQPLLTE
                     NKFQGCVAVQADQSEEQTDFLVDLASKNDFIKGVVGWIDLRDKNVSERLNHFSENKII
                     KGFRHVVQGEADDFMFRHDFRNGIRELKAYNFTYDILIFHRQLPAAIDLVHSFPDQAF
                     VIDHIAKPDIKSGNIKSWKKDIIEIAKAENVMCKISGMVTEANWNTWQTADLKPYLDV
                     IFENFSTHNLMFGSDWPVCNLAADYSKIVNTLEDYISQLSNQDQQQIWTNNAKSFYKL
                     YI"
     gene            complement(2848..4113)
                     /locus_tag="PLQ70_09500"
     CDS             complement(2848..4113)
                     /locus_tag="PLQ70_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34888.1"
                     /translation="MIAKWNEKKSYPWIVVGLLWFVALLNYLDRQMLSTMKPAMMVDI
                     PELAKAENFGLLMAIFLWIYAFMSPISGIIADRLNRKRMIIGSLFVWSGVTMAMGYAT
                     TFNQIYILRGVMGFSEAFYIPAALSLIADYHQGKTRSFAIAIHTTGIYLGQALGGFGA
                     TISKYFSWHFTFHSVGLLGVLYSLILIFFIEEKKTYFFDSTKKTSIKTEFKQLFKGLG
                     IVFGNISFWVLLFYFSAPSLPGWAVKNWLPTLFSETLHLDMALAGPIATVTISMSSFV
                     GVLIGGIVSDQWVMKHLKGRIYTSAIGLSLTIPALFLLGNCSSIEAIIVGGIFFGLGF
                     GIFDTNSMPILCQFVSPRYRATGYGIMNFVGISAGAVITEFLGKAADNGGMGQIFVLL
                     LFVVIAAIVLQLVSLNPKTINMTEKVTKS"
     gene            4408..>5333
                     /locus_tag="PLQ70_09505"
     CDS             4408..>5333
                     /locus_tag="PLQ70_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34889.1"
                     /translation="MNHKESYFLRFLYLTLMVFGFGCFSIYAQNNFAVADILANQLHN
                     NTKNQNMDLVYLQTSKGIYETEEDLWFKGYVLDAQYFYPSGRSKTLFVQLIADKTDQV
                     VWEKKYEIEDGFVDGHLFLKNDLEEGTYTLAAYSAHSFDKGAKEFYAAKKLEIKKEIS
                     TKVVAIPVEKDSILHFVTFPEGGKLVSGIESTLAFKAVNSKGLPVVVSGTLFENDIPL
                     LPIATNRAGMGGFDFIPDANNKYHITLNGSDKIYSIATIASSGKVLHLIGSNKDVVYF
                     KITQTNDLKEEKVYLRFQMRGVVYSVATGLLK"
BASE COUNT         1841 a          822 c         1050 g         1620 t
ORIGIN      
        1 gtttatctta aagaaggtga tgtaatcgaa ttgggaatgg aaagtttagg atctagtaaa
       61 caattggcag tagggtataa aaagtaaatt aaaaaggaaa aattaaaatg aaatatattg
      121 tatgtgaaaa acctcatgag tttaagttga aagaaaaacc catgccagaa cccaaagaag
      181 gtgaagtttt attgaaaata aaaagaattg gcgtttgtgg aactgacatt catgcttatg
      241 gcggaacaca accttacttt gaatatccta gagttttagg tcacgaattg gctgccgaat
      301 acattaaagg aaatgccaaa ggttttgaac caggtgataa agtgactttg ataccttatt
      361 ttaattgtgg agaatgtgtg gcttgtagaa acggattgac caattgttgt gccaatatta
      421 aagtgtttgg cgttcatatt gatggtggaa tggccgaata tataactgtt ccagagcaat
      481 atttactaca cagtcaaggt ttggattatg atgaattagc cttagttgaa ccattagcca
      541 ttgccgctca cggtgtaaga agagctgctg ttgaagccaa agatactgtt ttggtaatgg
      601 gcgcagggcc aattggaatt ggattgatcc agtttgctaa aattgcgggt gccaaagtta
      661 ttgttttgga tattaatgat ttcagattgg atttttgcaa aaaagaactt caagctgacg
      721 ctgtcataaa tccaatgaat gaagatgtaa ccgaaaggct taagcaaatt acaaatggag
      781 atatggtcaa tgtagttata gacgccacag gaaatagaaa tgtgatgaat ggtgctttta
      841 attatgttgc tcacggaggg cgatttgttt tggtagggct tcaaaaagga gaattgagtt
      901 tcagtcatcc tgaatttcat aaaagagaaa ccaccttgat gagtagccga aatgcaacca
      961 tcgaggattt tgaatttgtg atgaaatgtc tgaaatcagg aaaaattgac cctaaaaaat
     1021 acatcactca tagggctaat tttaccgata tgattgatgt ttttgatcaa tggattgatc
     1081 caaaaaataa tactatcaaa gcaattatag aattgaatta attcttaaaa aaatcattat
     1141 ggatttacat ttaaaaggaa aaatagttgt tgtttcgggt tcagcaggca aagaaggaag
     1201 tattggcgaa accattgtca aacgtttggc cgatgaaggc gctattccag ctcttgttga
     1261 tagaaatagc cgaggttttg gttatgctga agaacttaaa aaaagaggaa ttgatgccat
     1321 ttttgttcaa accgatgtta caaatccgga acaaatggaa aatgcggtca ataaaattgt
     1381 tgaaaaatac ggcagaattg acgcagtaat caataacgtc ggtgtcaatg atggtgctgg
     1441 attggattct agttatgaag agtttatgaa ttctttgaag ctaaatgtgg tgagctattt
     1501 tttattgacc aaatttgcgc ttccttatct aaaagcatcc aaagggaata ttctgaatat
     1561 tggttctaaa gtggctttaa caggacaagg aggcacatcg ggttatgctg ctgcaaaagg
     1621 gggagttctt ggtttgacca gagaatgggc agtcgattta attcagtttg gaattcgttc
     1681 taatgcaata attattgccg aaagttttac tccagcttat gacgaatggt tgaaaacgct
     1741 acccgatgga gaagcagttt tgcaaaaaat aaataaaagt attccgtttg aagctagaat
     1801 gaccaaagca gaagaaattg ccgatacagc actttttata gtttcggatc gttcttcgca
     1861 taccacagga caatttgttt gcgttgatgg aggttatatt catttggatc gtgctttaat
     1921 taatgatgta agtctttaaa ttgtatgaaa aaccgaattg attctcacca acatttttgg
     1981 aaattcgacc ccgtaagaga tagttggatt gatgattcta tgctaaaaat tcaaaaagat
     2041 tttttgccag aagatttaca gcctttgctg accgaaaata aatttcaagg ctgtgtagct
     2101 gtccaagccg atcaatcgga agaacagacg gattttttag ttgatttggc atctaaaaac
     2161 gattttataa aaggagtcgt tggctggatt gatttacgag ataaaaacgt gagtgaaaga
     2221 ttgaatcatt tttcggaaaa taaaataatc aaagggttta gacatgtggt gcaaggcgaa
     2281 gcggatgatt ttatgtttcg acatgatttt cgcaatggca ttcgtgaatt gaaagcgtat
     2341 aattttactt atgacattct tatttttcat cgacagttgc cagctgctat agatttggtt
     2401 cattcgtttc ctgaccaagc ttttgtaatt gatcatattg ccaaaccaga tataaaatcg
     2461 gggaacataa aatcttggaa aaaggatata attgaaattg caaaagcaga aaatgtgatg
     2521 tgtaaaattt caggaatggt aaccgaagcc aattggaata catggcaaac cgctgatttg
     2581 aaaccttatt tagatgttat ttttgagaat ttttcgaccc ataatttaat gtttggatcg
     2641 gattggccag tttgcaattt ggctgccgat tattcgaaaa ttgtaaatac actagaagat
     2701 tatatttcgc aactttcaaa tcaagatcaa caacagattt ggaccaacaa cgcaaaatca
     2761 ttttacaaat tatatattta agacttgaaa tgatagaata aaaaaaggga cacaaactat
     2821 gcgtcccttt tttttagttt tattttatta agatttggta actttctccg tcatatttat
     2881 tgtttttgga tttaagctaa ccaattgcaa aacaatggca gcaataacca caaatagcaa
     2941 taaaacaaat atttgtccca ttcctccatt atcagcagct tttcctaaaa actcagtaat
     3001 taccgctcct gccgaaatgc caacaaagtt cataatccca tatcccgttg ctcgataacg
     3061 tggcgaaaca aattggcaaa gaataggcat actgttggta tcaaagattc caaaaccaag
     3121 tccaaagaat attccaccaa caataatggc ttcgatacta ctgcaattgc ctaataaaaa
     3181 aagcgctgga attgttagag acaaaccaat tgcgctagtg taaattcgtc cttttaggtg
     3241 tttcattacc cattgatcag aaactattcc tccaatcaaa actccaacaa atgacgacat
     3301 cgaaatggta acagtagcga tgggcccagc aagcgccatg tctaaatgta aagtttctga
     3361 aaacaaagtg ggtaaccagt tttttacagc ccaaccaggc aaacttggag ccgaaaaata
     3421 aaagagcaaa acccaaaatg aaatatttcc aaacacgatt cccaaccctt tgaacagttg
     3481 cttaaattcg gttttaatgg atgttttttt ggtagaatca aaaaaataag ttttcttttc
     3541 ttctataaag aaaataagaa ttagactgta taaaacacca agcaaaccaa cagaatgaaa
     3601 ggtaaaatgc caagaaaaat atttagaaat tgtagcacca aaccctccta aagcttgtcc
     3661 taaataaatt ccagtggtgt gaatggctat ggcaaaagag cgcgtttttc cttgatgata
     3721 atcggcaatt aaagacaaag cagctgggat atagaaggct tcgctgaaac ccataacacc
     3781 ccttaaaata taaatttgat tgaaagtggt ggcataaccc attgccattg taacgcctga
     3841 ccaaacaaaa agactgccaa taatcattct ttttcggttg agtctgtcgg caatgattcc
     3901 cgaaatggga ctcataaaag cataaatcca aagaaaaatg gccatcaaga gaccaaagtt
     3961 ttcggctttt gctaattctg gaatatcaac catcatagct ggtttcatag tcgaaagcat
     4021 ctgacgatct aaataattca ataaggccac aaaccataac aagcctacaa caatccaagg
     4081 ataggatttt ttttcattcc attttgcaat catattgtct ttccaaataa aggccgaata
     4141 tacgaattca aatgcaactg aagggcatac ttttgtattg attagaattc aaaactaaaa
     4201 agaatttgac tggtgaattt tgcctgttat aactattttt acttctacta taaaaacaat
     4261 ttttgttatg tctttcaatt aggattagga atagattgcg ttttgttaat ggcacaattt
     4321 ttgataatct taagaatcat tttcttataa aataaaaaaa cttttgcaaa ataaaaacta
     4381 aaatacagtg tgactgtttc aaaatcaatg aatcataaag aatcctattt tttgaggttt
     4441 ttatacttaa ccctgatggt atttgggttt ggctgttttt ctatttatgc tcaaaacaac
     4501 tttgcagttg cagatatatt agccaatcaa ttgcataata atactaaaaa ccaaaatatg
     4561 gatttggtgt atttgcaaac cagcaaagga atttatgaaa cagaggaaga cctttggttt
     4621 aaaggatatg ttttggatgc tcaatatttt tatccttcgg gacgtagcaa aacattgttt
     4681 gtacaattga tagccgataa aaccgatcag gtagtttggg agaaaaaata tgaaatagaa
     4741 gatggttttg ttgatggtca tttgtttctg aaaaatgatt tggaagaagg gacttatacc
     4801 ctggcagctt acagtgctca ttcttttgac aagggcgcta aagaatttta tgcggcaaag
     4861 aagttggaaa tcaaaaaaga aataagcacc aaagttgttg caattcctgt tgaaaaagac
     4921 agtattctac attttgtaac ttttcccgaa ggaggaaaat tggtctcagg gatcgagagt
     4981 accttggctt ttaaagcggt caattcaaaa ggattgcctg ttgtggtttc gggaacattg
     5041 tttgaaaatg atattccttt gcttcctatt gcaactaacc gagcaggtat gggtggtttt
     5101 gattttattc cagatgccaa caataaatac catatcacac tcaatggttc tgataaaata
     5161 tatagtattg caaccatagc atctagtggt aaagtattgc atctgattgg tagtaataag
     5221 gatgtagtct attttaaaat cacgcaaacc aatgatttga aagaagaaaa ggtgtacttg
     5281 cgattccaaa tgcgtggcgt ggtgtatagt gtcgcaacgg gtttgctgaa gga
//
LOCUS       DAOSNN010000190         5302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15638_length_5302_cov_7.862779, whole genome shotgun sequence.
ACCESSION   DAOSNN010000190 DAOSNN010000000
VERSION     DAOSNN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15638_length_5302_cov_7.862779"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..325
                     /locus_tag="PLQ70_09510"
     CDS             2..325
                     /locus_tag="PLQ70_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008255719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HQE34890.1"
                     /translation="MIIYNVTTNIHESVHDQWMIWMQHKHIPEMLATGKFTSAKMTRV
                     LIEEEMGGVTYSVQYLTDSRETLQKYYQTEAEALREEGVRLFGDKMLSFRTELEVISE
                     FFQNN"
     gene            335..658
                     /locus_tag="PLQ70_09515"
     CDS             335..658
                     /locus_tag="PLQ70_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34891.1"
                     /translation="MTNNKMLSDFDVLIRGQLTVNLPITVIMLAVFFGLLEFADLSLR
                     LNLLIAFIFGWISWSILVKKWILWAKENNVSDERLLKIGKPGLLVWSIHTIETVTKKN
                     KNPWI"
     gene            705..1484
                     /gene="rsmA"
                     /locus_tag="PLQ70_09520"
     CDS             705..1484
                     /gene="rsmA"
                     /locus_tag="PLQ70_09520"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006258031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQE34892.1"
                     /translation="MEKVKAKKHLGQHFLKDESVAQNIADTLSLEGYEDVLEIGPGMG
                     VLTKYLLEKPINTYVIEIDAESVEYLGVHYDKLKDKIISQDFLKYDINEVFKRKQLAI
                     IGNFPYNISTQIVFKVLEYKNQIPEFSGMFQKEVAERICEKKGSKAYGILSVLAQAFY
                     DTEYLFTVSEHVFDPPPKVKSGVMRMRRKEDYSLPCSEKLLFTVVKTAFQQRRKTLRN
                     SLKTLNLSDNLREDEVFNLRPEQLSVAQFIELTQKIEADGV"
     gene            1474..2823
                     /gene="mgtE"
                     /locus_tag="PLQ70_09525"
     CDS             1474..2823
                     /gene="mgtE"
                     /locus_tag="PLQ70_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQE34893.1"
                     /translation="MEFKVSKELIHELEQFIQSKDDQQLEVLLNDLHHADIAEILDEL
                     DIDEATYIFKVLDSEKTAEILLELEDDLREKILSRLSPKEIAEELDELETNDAADIIA
                     ELSQSKKQEVISELQDVEHAKDIVDLLRYKEDTAGGIMHKELVKVNENWDVFMCIKQM
                     RIQAENISRVHSIYVVDDEDRLLGRLSLKDLLTTPSKKPISEVYIKKLNSVMVDTEDV
                     EVARIMQKYDLEAIPVTDELGRLVGRITIDDIVDVIKDEADQDYQLAAGITHDVEAGD
                     SILELTKARLPWLLIGMVIEIVASFVLKSNESAFQKYSTLIIFVPLLSATAGNIGVQA
                     SAIVVQGLANGTLKEFSRDYFSKELAVAMISGSIISLLLLGYHSFMYQQYQVGLAISI
                     SMVVVILFAATLGTLVPLFLHKNKIDPAIATGPFVSTTNDVFGIILYFWIAQMILGF"
     gene            2836..3612
                     /gene="proC"
                     /locus_tag="PLQ70_09530"
     CDS             2836..3612
                     /gene="proC"
                     /locus_tag="PLQ70_09530"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQE34894.1"
                     /translation="MKVHIIGGGNLGVSVALGLARFSKDNQITITRRNTTSILYLEEL
                     GITVSKDNKHGIQEADVVILTIKPYQVDTVLAEILPIISNKVIASAVSGLSIENLQKK
                     IGDSNYAIRIMPNIAAQFGASATCIAFNEKHTEQAQNVVALFQDLGTAPIIDEKLMDA
                     ATVLAASGTAFALRYIRASMQAGIEIGFDWKTALDISAQTVKGAAEMILEENIHPEQL
                     IDRVTTPQGCTIAGLSEMETHGFSSSLVRGIKAALKKIKG"
     gene            3795..4691
                     /locus_tag="PLQ70_09535"
                     /pseudo
     CDS             3795..4691
                     /locus_tag="PLQ70_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
     gene            5145..>5302
                     /locus_tag="PLQ70_09540"
     CDS             5145..>5302
                     /locus_tag="PLQ70_09540"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HQE34895.1"
                     /translation="METTITEHWNKPNNPIPIFKVDPILVLILGFITCGLYHIYWNIK
                     VAEVLNAVT"
BASE COUNT         1818 a          909 c         1087 g         1488 t
ORIGIN      
        1 aatgatcatt tacaacgtta ccaccaacat acacgaaagc gttcacgacc aatggatgat
       61 ttggatgcaa cacaaacaca ttccagaaat gctggctacg ggaaaattta cgtcggccaa
      121 aatgacgcgg gttttaattg aagaagaaat gggaggtgtt acgtattccg ttcaatattt
      181 aacggatagc agggagactt tacaaaaata ttaccaaaca gaagcagaag cattgcgtga
      241 agaaggagta cgattatttg gagacaaaat gctttctttc cgaactgagt tggaagtgat
      301 ttcggaattt ttccagaata attaaaaagc aaaaatgaca aataacaaaa tgctttcaga
      361 tttcgatgta ctcataagag gacagcttac tgtgaatttg ccaattactg taattatgtt
      421 ggctgttttt tttggattac ttgaatttgc cgatttatct ttaagattaa atttattgat
      481 agcttttatt tttggctgga tttcttggag cattttagta aaaaaatgga ttctttgggc
      541 aaaagaaaat aatgtttcgg atgaaagatt attaaaaatt ggaaagccgg ggttgttggt
      601 ttggagtatt cataccatag aaacggtaac aaaaaagaat aaaaatcctt ggatataaat
      661 aaaagaataa aacctttgcg cctttgtggt ttaaaaaaac aattatggaa aaagtaaaag
      721 ccaaaaaaca cctgggacaa catttcttga aagatgaaag cgtggcacaa aacattgccg
      781 acaccttgag tttggaagga tacgaagatg ttctggaaat tggtccggga atgggcgttt
      841 tgaccaaata tttattggaa aaaccaatca atacctacgt gatcgaaatt gatgccgaat
      901 cggtagaata tttgggtgtt cattatgata aattgaaaga caaaatcatt tcccaagatt
      961 tcctgaaata cgacatcaac gaggttttca aaagaaaaca gttggctatc attggcaatt
     1021 ttccatacaa tatttccacg caaatcgtat tcaaagtatt ggaatacaag aatcaaattc
     1081 cggaattctc gggaatgttc cagaaagaag tggcggaacg catctgcgaa aaaaagggaa
     1141 gtaaagccta tggaattctc tccgttttgg cgcaagcttt ttatgatacc gaatatttgt
     1201 ttacggtaag cgaacacgtt tttgatccac caccaaaagt aaaatcgggc gtgatgcgaa
     1261 tgcgcagaaa agaagattac agtttgcctt gcagcgaaaa gttgttgttc acggtcgtga
     1321 agaccgcttt tcagcaacgc agaaaaacct tgagaaatag tttaaaaacg ctaaatttgt
     1381 ccgataattt gagagaagac gaagttttta acctgcgtcc agaacaactt agcgtagcac
     1441 aatttataga attgacccaa aaaatagaag cggatggagt ttaaagtcag caaagaacta
     1501 attcacgaat tagagcaatt tattcaaagc aaagacgacc agcaactgga agttttattg
     1561 aatgatttgc accacgctga tattgccgaa attctggatg agcttgatat tgacgaagcc
     1621 acttatattt tcaaggtttt ggattctgaa aaaaccgctg aaattcttct ggaattagaa
     1681 gacgatttgc gggaaaaaat attaagccga ctttcgccaa aagaaattgc tgaagaactt
     1741 gatgagcttg aaactaatga cgctgccgat attattgccg aactttcgca aagcaagaag
     1801 caggaagtaa tatcagagct tcaagacgta gaacacgcca aggatattgt cgatttgttg
     1861 cgttacaaag aagataccgc tgggggaatt atgcacaaag agttggtgaa agtcaacgaa
     1921 aattgggatg tatttatgtg catcaagcag atgagaatcc aagccgaaaa tatttcgaga
     1981 gtacattcta tttatgtagt ggatgatgag gatagattat tagggcgttt gtcgctcaag
     2041 gatttgttga ccacaccctc aaaaaaaccc atcagcgaag tttatatcaa aaaattaaac
     2101 tccgtaatgg tcgataccga agatgttgag gtggctcgta tcatgcaaaa atacgatttg
     2161 gaagccattc ctgtaacaga tgaattaggg cgattagtag ggcgaatcac gatcgatgat
     2221 atcgtagatg taattaagga cgaagccgat caggattacc aattggctgc aggtattacg
     2281 catgacgttg aagcaggtga tagtattctt gaattgacaa aagcacgctt gccttggctt
     2341 ttgataggaa tggtgattga aattgtcgct tcgtttgtgt taaaaagcaa tgaaagcgct
     2401 ttccaaaaat attccacttt gattattttc gtgccgttac tttcggcaac tgcgggaaat
     2461 attggggtgc aagcctcggc aattgtggtg caaggtttgg ccaacggaac tcttaaagaa
     2521 ttcagccgag attattttag taaagaactt gctgtggcaa tgatttcggg tagcatcatt
     2581 tccttgttgt tacttgggta tcattcgttt atgtaccagc aatatcaagt gggattagcc
     2641 atttccattt ctatggtcgt tgttatttta tttgcggcaa ctttgggtac cttggtgcca
     2701 ctttttctcc acaagaacaa aattgatccc gctatagcaa cagggccatt cgtttccact
     2761 acaaatgacg tattcggaat tattctttat ttttggattg cacaaatgat tttaggtttt
     2821 taatatacac aaaaaatgaa agtacacatt ataggaggag gaaatcttgg cgtttccgta
     2881 gcattaggat tagccagatt ttctaaggat aaccaaatta caattaccag aagaaacaca
     2941 acaagtattc tgtatttaga agaattagga ataacagttt caaaagataa caaacacgga
     3001 attcaagaag ccgatgttgt tattttgacc atcaagccgt atcaagtaga tactgttttg
     3061 gccgaaattc ttcctataat ttccaataaa gtcattgctt cggcagtaag tggtttgtcg
     3121 attgaaaatt tacaaaaaaa gataggcgat tccaattatg ccattcgaat tatgcccaat
     3181 attgcggctc aatttggcgc ttctgcaacc tgtattgctt tcaacgaaaa acataccgaa
     3241 caagcacaaa atgtggttgc tcttttccaa gatttgggaa cagcacctat cattgacgaa
     3301 aaattgatgg atgcagctac cgttttggca gcaagcggaa cggcttttgc tttgagatac
     3361 atccgtgctt cgatgcaagc cggaatcgaa ataggtttcg actggaaaac agctttagat
     3421 atttcggctc aaacggttaa aggagctgcc gaaatgattt tggaagaaaa catacatcca
     3481 gaacaattaa ttgaccgagt aacaacgcca caaggctgca cgattgcagg tttgagtgaa
     3541 atggaaaccc acggattcag ttcctcattg gttcgcggaa tcaaagccgc tttgaagaaa
     3601 atcaagggct aaagaattgc agatttttga ttaacgattt ttgatttcag atttggcgtt
     3661 aataattaaa tgattgttca atttcaaaaa gctcgcacat ctgcaatcaa aaatcgttaa
     3721 tcttcattca aaaatcaaaa aaattgaaac ctgaaaatat taaaatcctc cacatcgaca
     3781 gcaaccatcc cataatgatg gcacaattgc agcaagcagg atttaccaac cacgaagatt
     3841 tcacttcttc caaagcagaa attgaaacca aaatccaaga ttatcaaggt gtcgttatcc
     3901 gaagccgatt caaaatcgat aaaacatttc tggacaaagc caccaatttg caattcattg
     3961 ccagagtggg tgctgggttg gaaagtattg attgcgacta cgcattatcc aaaaatatcc
     4021 aacttattgc cgcaccagaa ggaaaccgaa acgccgttgc cgaacacagt ttgggaatga
     4081 ttttgtcgct tttcaacaat ttgaatcaag ccgacaacga agtaaaatca ggacattgga
     4141 accgggaaaa aaaccgtggt cacgaattgg atggtaaaac cgttgggatt ataggttacg
     4201 gcaatatggg aaaatctttt gccaagaaac tcagaggttt tgaggtagaa gttttgtgtt
     4261 atgacatctt ggaaaacgtt ggcgatgaaa atgcacgaca agtttcactc gcacaattac
     4321 aacaaaaaac cgatgtttta agccttcaca ttccttggac acccgaaacc gacaagatgg
     4381 tcaatgccga ttttatcaat gcttttgcca aacctttttg gataatcaac acttccagag
     4441 ggaaaaacat tgtcactgcc gatttggtcg aagcgatgaa aagcaagaag attctcggtg
     4501 ctggtttaga cgttttggaa tatgaaaaat tgtctttcga aacccttttt caagacaaag
     4561 acacccccga agcttttcaa tatttattga atgccaaaaa cgtaatcctg acgccacaca
     4621 ttgccggctg gacttttgaa agtcacgaac gattagcaca ggttattgtg aataaaatta
     4681 aggcgaaata ttatggtgag gtcaaagaaa aatcaattga aaaccgagtg actggaattg
     4741 gaggtttctt cttcaaggct gagaatcctc aaaaacttgt ggattggtat agtaagcatt
     4801 taggattaaa aacggatgat tatggttcca ctttttggtg gaaagacaaa aacggaaaag
     4861 actgttctac ccaatggtcg ccatttgcgg atgacacaaa ttacttcgat ccaagtaaaa
     4921 aacaatttat gcaaaatttt cgtgtggttg atttagaaaa attattagaa aaattattac
     4981 acgaaggtgt aacaattata ggggaaatgg agacatatga ttatggaaaa tttggttgga
     5041 ttctcgacat cgaaggaaat aaaattgaac tttgggaacc aattgataaa gcatttcaat
     5101 aatttttata attttacaaa aatcaactaa tcaacaatca atctatggaa accacaatca
     5161 cagaacattg gaacaaacca aacaacccaa ttcccatatt taaagtcgat cctattttag
     5221 tattaatact cggatttata acctgcggat tgtatcatat ttattggaat attaaagtag
     5281 ccgaagtact aaacgcagtc ac
//
LOCUS       DAOSNN010000191         5292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15684_length_5292_cov_3.886003, whole genome shotgun sequence.
ACCESSION   DAOSNN010000191 DAOSNN010000000
VERSION     DAOSNN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15684_length_5292_cov_3.886003"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /gene="dnaA"
                     /locus_tag="PLQ70_09545"
     CDS             <1..1405
                     /gene="dnaA"
                     /locus_tag="PLQ70_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQE34896.1"
                     /translation="WENCLAFIKDNIQEQAFKTWFEPIKSVELTDNALYIQVPSKFFY
                     EWLEEHYVKLLKVALTKELGKNAKLLYKIKMENTYGNKQPFTEQLPSANRVPMKPQDV
                     DAPYKNLNPELKNPFVIPGIRNLKIESQLNPNYSFDNFLEGDSNRLARSAGMAVANKP
                     GGTSFNPLLIFGGVGLGKTHLAHAIGVEIKDKYPEKTVLYISAEIFTQQYIDSVKKNN
                     RNDFIHFYQLIDVLIIDDVQFLSGKSGTQDVFFHIFNYLHQNGKQVILTSDKAPVDMQ
                     DIEQRLLSRFKWGLSAELHQPDYETRISILKNILYRDGVEIPDEIIEYVARNIKSNVR
                     ELEGAIISLIAQSSFNKKEVTLELAKNVVEKFVKNVKREISIDYIQKIVSDYFQLDLE
                     TLQSKTRKRHIVQARQLAMFFAKKFTKASLANIGSQIGDRDHATVLHACKTVDNLVAT
                     DKQFKKFVEDINKKLTL"
     gene            1414..1881
                     /locus_tag="PLQ70_09550"
     CDS             1414..1881
                     /locus_tag="PLQ70_09550"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HQE34897.1"
                     /translation="MPIKILMVCLGNICRSPLAEGILASKLPKKEFHIDSAGTGSWHI
                     GRQPDERSIAVAKKYRIDISNQKGRQFSKKDFDAFDYIYVMDNSNYQDVIRLATTIEQ
                     KNKVQLILNELFPNENVDVPDPYYGVANGFEVVYKMLDESCEIIAQKLLKKHS"
     gene            1917..2642
                     /locus_tag="PLQ70_09555"
     CDS             1917..2642
                     /locus_tag="PLQ70_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQE34898.1"
                     /translation="MKPNLFLGKLYLIPTTLGEEGDPNDVLPQTIKRSIDFIDDYIVE
                     NEKTARRFIKSIQPQKVQASLRLSALNKHTEISEHAKMLQACMEGKNVGLMSEAGCPG
                     VADPGAVIVKLAHEKGIQVIPLVGPSSILLAIMASGMNGQSFAFNGYLPIDKGEKKSA
                     LKSLEKLSQDKNQSQIFIETPYRNNKMLEDIIQALNPSTYICVATDISMPTEFIKTMR
                     ASEWKKTQVDLHNRPTIFIIHKM"
     gene            complement(2705..3343)
                     /locus_tag="PLQ70_09560"
     CDS             complement(2705..3343)
                     /locus_tag="PLQ70_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HQE34899.1"
                     /translation="MINKWSFYTSLTLIIAFLSSGFKPSDIDSNQWFLTNETDGTHYL
                     FPSQKQTDYSNLNVPYTGKFFIGYKEAIAFKESQGKYKKVNTLGYMGKYQFGKETLKT
                     IGIHDSNHFLNNPRIQEKAFVALIAKNKSELKDIIQKYEGQVVAGVLVTESGILAAAH
                     LGGVGSVKKFFKHDGKRYFKDAYGTSIRSYMREFGGYETSGIVADSKASVKL"
     gene            complement(3443..4303)
                     /locus_tag="PLQ70_09565"
     CDS             complement(3443..4303)
                     /locus_tag="PLQ70_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007650844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HQE34900.1"
                     /translation="MAQNSFDNFFKPSDSLNKKRKNAVFVTEAVLASGALVGLNQMWY
                     ADYPKSNFHFLNDNAQWMQMDKLGHLYSSYHIGRLGADALKWSGASKKNQLIYGASLG
                     FVFLTAVEVMDGHSAEWGASIGDVFANATGTALYVSQELVWNEQRIIPKFSFHTTQYA
                     NYRPDLLGNSLSEQILKDYNGQTYWFSVNLYSFAKKSKIPKWFNIALGYGAEGMLGEN
                     DTKNNLILATKQENYRQFYLSLDLDLTKINSKSHFLKTVFSVLNTVKIPAPALEYSPQ
                     RGLKFHALYF"
     gene            complement(4360..>5292)
                     /gene="mltG"
                     /locus_tag="PLQ70_09570"
     CDS             complement(4360..>5292)
                     /gene="mltG"
                     /locus_tag="PLQ70_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQE34901.1"
                     /translation="SQTEVYVFVPTGSTYEEVKKIIEPFVDNMDRFEMVANKRSYPEN
                     VKSGRFLFTKGMNSYQLVKALRSNIPVKLAFNNQERLEDFAVRVSSQIEPDSLMLMNT
                     FKDSLFLKENGFTEENVFAMFIPNTYEVYWNISAVKFRDKMIKEYHSFWNKERIAKAS
                     AQGLTPVEATILASIVHKESVKKDERPRIAGVYLNRLKIGMPLQADPTVIYAIKKKDN
                     DFDQVIKRVFYNDLFLTSPYNTYKVIGLPPGPIAMPDVTALDAVLDPEKHNFIYFCAS
                     VERFGYHEFAATLPEHNVNATKYADWINSQGVNR"
BASE COUNT         1820 a          976 c          850 g         1646 t
ORIGIN      
        1 atgggaaaac tgtctagcat ttataaagga caatattcaa gagcaagctt tcaaaacttg
       61 gtttgaaccg attaagtcag ttgaacttac agataacgct ttgtatattc aagtaccaag
      121 taaatttttc tacgaatggc ttgaagaaca ttatgtgaaa ctgctaaagg ttgccttaac
      181 caaagaactc ggtaaaaatg caaagttact ctataaaatc aaaatggaaa acacttatgg
      241 caataaacaa ccgtttactg aacaattgcc tagtgcaaat cgtgtgccca tgaaaccaca
      301 agatgttgat gccccataca aaaatctaaa tccagaatta aaaaaccctt ttgtaattcc
      361 aggtattcga aatttaaaaa tcgaatcaca actgaatccc aattatagtt ttgacaattt
      421 tttagaaggt gattctaata gattagctcg ttcagcaggt atggctgtgg ccaataaacc
      481 gggcggaaca tcctttaatc cattattgat ttttggtggt gttggcttag gaaaaaccca
      541 tttagcacac gcaattggtg ttgaaattaa agataaatac ccagaaaaaa cagtcttata
      601 tatttcggcc gaaattttca ctcaacaata tattgattct gtaaagaaaa ataatcgaaa
      661 tgattttatt catttctacc aacttatcga tgttttaatc attgacgatg ttcaattcct
      721 atctggaaaa tcaggaactc aagatgtgtt tttccatatt tttaattatt tgcaccaaaa
      781 tggcaaacaa gttatcctaa cttcggataa agcgccagta gatatgcaag acattgaaca
      841 acgactgtta tcccgtttca aatggggatt gtcggcagaa ttgcatcaac ccgattatga
      901 aaccagaatt tcaatcttaa aaaacatact ttatcgtgat ggcgttgaaa ttccagatga
      961 aatcatagaa tatgtagctc gaaatatcaa atcgaatgta agagaattag aaggtgctat
     1021 aatttcctta atagctcaat cgtctttcaa caaaaaagaa gtaactcttg aactagccaa
     1081 aaatgttgtc gagaaattcg tcaagaatgt aaaaagagaa atttctattg attacattca
     1141 aaaaattgtt tctgattatt ttcaattgga tttagaaaca cttcaatcta aaactagaaa
     1201 aagacatatt gttcaagccc gtcaattagc tatgtttttc gctaagaaat ttaccaaggc
     1261 atcattagca aatattggtt cccaaatagg tgatcgcgat catgctaccg ttttacacgc
     1321 ttgtaaaacc gttgacaatt tagtggccac tgacaaacaa ttcaaaaaat ttgtcgagga
     1381 catcaataaa aaattaacgc tataaacctc ggtatgccca tcaaaatttt aatggtttgc
     1441 ttaggtaata tttgcaggtc tcctttggcc gaaggaatat tagcttcaaa acttcccaaa
     1501 aaagaattcc atattgattc ggctggaaca ggttcttggc acattggccg acagcctgat
     1561 gagcgttcta ttgcagtagc aaaaaaatac agaatagata tttcaaatca aaaaggccga
     1621 caattctcta aaaaagattt tgatgctttt gactatattt atgtgatgga taattccaac
     1681 tatcaagatg taattagact tgccaccacc atagaacaaa aaaacaaggt acaactcatc
     1741 ttaaatgaat tgtttcccaa tgaaaatgtt gatgtacccg atccatatta tggcgttgcc
     1801 aatggttttg aagtagttta taaaatgcta gatgaatctt gtgaaatcat cgcacagaaa
     1861 ctcctaaaaa aacactcgta actatttttt ttcagatttt tctaaaaacc aaatttatga
     1921 aacccaacct ttttttagga aaactatatt taattccaac tactttagga gaagaaggcg
     1981 acccgaatga tgttttgcca caaaccataa aaagaagcat tgatttcata gatgattata
     2041 tagtagaaaa cgagaaaaca gctcgaagat tcatcaaaag tattcaacct caaaaagttc
     2101 aagcctcact tagacttagt gctctaaaca aacacaccga aatatccgaa cacgccaaaa
     2161 tgctacaggc ttgtatggag ggaaaaaatg tgggtttaat gagtgaagca ggttgtccag
     2221 gagttgccga tccaggcgct gtgatcgtaa aattggctca cgaaaaagga attcaagtca
     2281 taccactcgt tggtccatcc tctattttat tggcgattat ggcttctgga atgaacggac
     2341 aaagttttgc tttcaatggt tatcttccta ttgataaagg agagaaaaaa tcggctttaa
     2401 agagtctgga aaaattatct caagacaaaa atcagtccca aatttttatt gaaactccat
     2461 atcgaaacaa caaaatgcta gaggatatca ttcaagcttt gaatccttcg acttatattt
     2521 gtgttgctac agacatctct atgccaactg aatttatcaa aacaatgcga gcatctgaat
     2581 ggaaaaaaac gcaggttgat ttacacaatc gccctacgat atttattatt cataaaatgt
     2641 aaaaatacta ctcgaaaaaa aaccacgcta aggtggtttt attatttttt acacaaaact
     2701 gaaattacaa tttcacactt gctttactat ctgcaacgat tcctgaagtt tcataacctc
     2761 caaattctct catataactt cgtatcgaag ttccataagc atctttgaaa taacgctttc
     2821 catcgtgctt aaaaaacttt ttaactgaac ctactccacc aagatgagca gccgccaaga
     2881 tacccgattc cgtaacgaga acccctgcga caacttgccc ctcgtatttt tggataatat
     2941 ctttcaattc tgacttgttt ttggctataa gtgccacaaa ggctttttct tgaattctag
     3001 gattattcaa aaaatgattg ctatcgtgaa ttccaatggt ttttaaggtt tccttaccga
     3061 actggtattt tcccatatac cctaaagtat tcactttttt atattttcct tgagattctt
     3121 tgaaggcaat cgcttctttg taaccgataa aaaattttcc agtataagga acattcaaat
     3181 tcgaataatc ggtttgtttt tgtgatggaa ataagtagtg agttccatct gtttcattcg
     3241 ttagaaacca ttgattggaa tctatatcgg aaggcttaaa acctgagctt aaaaaagcaa
     3301 taattaaagt caaactagta taaaaagacc atttgtttat cataaattgt ttttcttcaa
     3361 aacgctgtcc cgttatgaaa tttctacggt gcaaatatac aataaaaaat atttatactg
     3421 attatcaagt agttaaaaaa atctaaaaat acaaggcgtg aaatttcaat cctcgctgag
     3481 gcgagtattc aagggcaggc gcagggattt tgaccgtatt taaaactgaa aaaacagtct
     3541 tcaaaaagtg tgattttgag ttaatttttg tcaaatctaa atccaaactt aggtaaaatt
     3601 gtcggtaatt ttcttgtttt gtagctaaaa taagattgtt tttagtgtcg ttttcaccca
     3661 acattccttc ggctccataa cccaaagcaa tattaaacca ctttggaatt ttcgattttt
     3721 tggcaaaaga gtataaattt accgaaaacc aataggtttg tccgttgtaa tcttttagga
     3781 tttgttctga gagtgaattt cctaataaat caggtctata attagcatat tgagtggtat
     3841 gaaaagagaa ttttggaatg atgcgttgtt cgttccaaac cagttcttgc gaaacataca
     3901 acgctgttcc tgtagcattg gcaaaaacat cgcctatcga ggctccccat tcagcagaat
     3961 gaccatccat aacctcaact gcagtaagaa acacaaaacc caaactggca ccgtaaatca
     4021 gttgattttt tttgctggca ccactccatt tcaaagcatc tgcacccaat cgaccaatat
     4081 gataggaaga ataaagatgt cctaatttat ccatttgcat ccactgggca ttgtcattga
     4141 ggaaatgaaa attagattta ggataatcag cataccacat ttggttcaag cccaccaatg
     4201 caccagatgc caaaaccgct tcagttacaa atacagcatt cttccttttc ttatttaagg
     4261 aatcagaagg cttgaaaaaa ttatcgaatg aattttgcgc caaaacattt tggaacacca
     4321 aaagaaacaa aaaacaaatt gtctttttgt aactcaaaat tacctattta caccctgact
     4381 attgatccaa tcagcatatt ttgtagcatt tacattgtgc tctggtaaag tagcggcaaa
     4441 ttcgtgataa ccaaaacgct ctacactagc acagaaataa ataaagttgt gtttctcggg
     4501 atccaaaacc gcatcaagag cagtaacgtc tggcatagcg atgggtcctg gcggaagtcc
     4561 aattactttg taagtattat aaggtgaggt taaaaacaaa tcattataaa aaactctttt
     4621 gataacttga tcaaaatcat tgtctttctt ttttatggca taaatcacag tgggatcagc
     4681 ctgaagtggc ataccaattt tcaaacggtt caaataaaca cctgcaattc taggtctttc
     4741 gtcttttttg acagattctt tatgcacaat tgaagctaaa attgttgctt caacaggagt
     4801 tagcccttgt gcggatgctt ttgctattcg ctctttattc cagaagctgt ggtattcctt
     4861 gatcatttta tcacggaatt taacagctga aatattccaa tacacctcat atgtattagg
     4921 aataaacata gcaaatacat tctcttcggt aaaaccattt tctttcaaaa aaagagaatc
     4981 cttgaaggta ttcatcaaca tcaaactatc gggctcaatt tgcgaactca ctcttactgc
     5041 aaaatcctct aatctttctt gattattgaa agccaatttc acaggaatat tgcttctcag
     5101 agcttttacc aattggtaac tattcattcc ttttgtaaat aaaaagcgcc ctgattttac
     5161 attttcggga tagcttcttt tattggctac catttcaaaa cgatccatat tatcaacaaa
     5221 aggctctatt atttttttga cttcttcata agttgaacct gttggcacaa atacatatac
     5281 ttcagtttga ga
//
LOCUS       DAOSNN010000192         5236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15892_length_5236_cov_4.344914, whole genome shotgun sequence.
ACCESSION   DAOSNN010000192 DAOSNN010000000
VERSION     DAOSNN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15892_length_5236_cov_4.344914"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PLQ70_09575"
     CDS             <1..717
                     /locus_tag="PLQ70_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34902.1"
                     /translation="TVTPSTPSSTTPTVTVPVNSTVINKGIVPASSFVSTPAGGTFAW
                     TNSNTAIGLAASGTGNVPSFTATNTGTTALVATITVTPTVNGLKGTPSSYTITVNPTP
                     PVTNAITLTGSSSGSTKSHNAGKNCMSCHTPGTSAGSKGVWVIGGTVYTSSAGSSIAK
                     NLVIKFYTGPNGTGTLKYILNSDALGNLYSANSVDFTGGLYPAITGATTTKYMGSPIT
                     NGACNSCHTGGSGTSRIWAN"
     gene            823..1284
                     /locus_tag="PLQ70_09580"
     CDS             823..1284
                     /locus_tag="PLQ70_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34903.1"
                     /translation="MKQKTITLFALMLMLTTMQSCTENEYGSITDSKSGSTESHNMGQ
                     NCMNCHKPGGGEAPAWKVAGTVYNEALTATNSNATVKLYTGPNETGILKYTIQVDAKG
                     NFYTTSAIDFTGGLYPSVTGATSTYSMSTPIETGACNSCHNGVIKSKIWTN"
     gene            complement(1395..3902)
                     /locus_tag="PLQ70_09585"
     CDS             complement(1395..3902)
                     /locus_tag="PLQ70_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE34904.1"
                     /translation="MFFCFVFALNLKAQDGSKAIALKTILQSIEKQHHVYFNYIENEI
                     VVIKIEPPKRSLSLNDKIVYLEKKTNLLFDKVDSQFITISSKKEVELICGYVFSKNDN
                     LPQESANIQILNGSHTTTDKKGYFEIKKENATEIEISHVGFRSQKIAVANLKNKNCLK
                     IFLELDVLELDNVMANHYLASGISKTTEGTFVVKPKKLGILPGLIEPDVLQTMLQIPG
                     IYTADESLSSINVRGGTHDQNLFLWNGIKMYQTGHFFGLISAFNPNLPSTVSISKNGS
                     SAFYGESVSSVVDISSDPNKFEKPQFSAGINMINADIYSKFNINKNGFLEIAARKSIT
                     DLVKTPTYTKYYNKAFQNTTVSDFATKQNIDYQNDEKFNFYDITLKYSQKIGEKDHLI
                     LDLIAINDQLDVNQKATINEIVQSENNRLYQKNHGANLLWKRNWNTNNSSKISIFSSS
                     YELDAEKNKIQDNQIVKQENTLLDNGLKIENNHSITSKFSFNNGYQFNEIGSTNLDQV
                     NTPAFYRKIKEVLRTHALIAEGKYKDTLSKIFFNVGMRLNYIEKFKKYLFEPRFQFNY
                     GISNSLSLEILGEFKSQNCFQVIDLQGDYFGVEKRRWILSNNSTVPIQKSKQASIGVS
                     YNKNNWLITLDNFYKKVTGINSSGQGFQNQLEFAKINGDYEVFGSEILVQKKINHFIT
                     WLSYAFNDNNYHFPGIEQTEFSNNFELNNVVSWAGTYDIGNLKIALGSKWYSGRPETT
                     PIGIDYSDSLNPTIEYNTPNNKNLENFFQVNFSTTYRWESSNNTQYKLGLSIINVFNK
                     QNEISEYYRINTHTNSIEDVKAYSLERTPNLSFRVIF"
     gene            complement(3930..4829)
                     /locus_tag="PLQ70_09590"
     CDS             complement(3930..4829)
                     /locus_tag="PLQ70_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HQE34905.1"
                     /translation="MEKNYILTKWLNDELTDAELAEFKADPDFEKYEKIKNYSAQLKV
                     TDFDEAKVLENILQHKKTTPKVIPLYKNWIFRVAAILVIALGITFTVQNFSSETQLAL
                     NGARTTFSLPDHSEVVLNAGSEIEYKKWNWDNNRNLQLKGEAYFKVAKGEKFEVKTTL
                     GKVTVLGTQFNVKARKNRFDITCFEGRVKVNYKDKEIILTPGKSVAFENGKQINLLVD
                     NQKPEWLENKIAFSKENLRNILDEIQRQYNLTIEVQADYPSELFTGKIPLNNLDVALE
                     IIATTYHLEPNKISPNKIIFEGK"
     gene            complement(4875..>5236)
                     /locus_tag="PLQ70_09595"
     CDS             complement(4875..>5236)
                     /locus_tag="PLQ70_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQE34906.1"
                     /translation="NCTSVPVEKAKSYIYTIANNSSLNEIKHKKVVLEYENNFTGADK
                     TNENPEFILEEKQFKNKLLKAIEDLNETQRVAFLMHRIDQKKYSEIAEELNISVKAVE
                     KRIHLALVELRKTIDFK"
BASE COUNT         1596 a          865 c          884 g         1891 t
ORIGIN      
        1 acagtaacac cgtcaacacc tagttctacg acaccaacag ttacagttcc agtaaacagt
       61 acggttatca ataaagggat tgtgcctgca tcgagttttg ttagtacacc agctggcgga
      121 acatttgctt ggaccaattc aaatacagct attggtttag ccgcaagtgg tactggaaat
      181 gtgccttctt ttacggcaac aaatactgga actacagccc tagtagctac aattacggtt
      241 actccaacgg tcaacggact taaaggaact ccttcttctt atactattac tgttaaccca
      301 actccaccag ttacaaatgc aataacgctt acaggtagtt cttctggctc aacaaaaagc
      361 cataatgcag ggaaaaattg tatgagttgt catacccccg ggacttcggc aggtagtaaa
      421 ggagtatggg taattggagg gacggtttat actagttctg ctgggtcttc aattgccaaa
      481 aatttggtta ttaaatttta tacagggcca aacggaacag ggactttaaa atatatacta
      541 aatagcgatg cacttggaaa tctatattct gccaattcgg ttgattttac gggaggctta
      601 tatccagcaa ttactggagc aacgaccaca aaatatatgg gttcccctat aacaaatgga
      661 gcttgcaaca gttgccatac gggaggttca gggacttcca gaatttgggc taactaatta
      721 aaatagatga aaaataagca gtaatacaaa gtatgttgat tgctgcttat tatgttttat
      781 agcaaaaaat aatttcaata attaataatt aaaaacaata aaatgaaaca aaaaacaatt
      841 acgctttttg cattaatgct aatgcttact acaatgcagt catgtacaga aaatgaatat
      901 ggctcaataa cagatagtaa atctgggagt actgaaagtc ataatatggg gcaaaactgt
      961 atgaattgtc acaagcctgg aggaggagaa gctcctgctt ggaaagttgc tggaacggta
     1021 tataacgaag cattgacggc taccaattcg aatgctactg taaaacttta tacaggtcca
     1081 aatgaaacag gaattttgaa atatacgatt caagtagatg ccaaaggaaa cttttatacc
     1141 acttcagcta tcgattttac aggaggtttg tatccttcag ttacaggagc cacttcaaca
     1201 tattctatgt caactcctat tgaaactggt gcttgcaaca gctgtcacaa tggggtgatt
     1261 aaaagcaaaa tatggactaa ctagaaaagg tattttgctt tttatagtga aaatagcatt
     1321 aaaaaagaaa ctgtctctta gttcgaggca gtttttttat gggaaaacga ataattcttt
     1381 ttaaaaagtt gggattaaaa aatcactcta aagctcaaat taggagttcg ttcaagcgag
     1441 taggctttta catcttcaat agagttggta tgggtattaa tcctgtaata ttcgctaatt
     1501 tcattttgtt tgttgaaaac atttattata gataatccca gtttatattg agtattattt
     1561 gaactttccc atctgtatgt tgtcgaaaag ttaacttgaa aaaaattctc aagattttta
     1621 ttgttgggag tgttgtattc gattgtagga tttaaagaat cgctatagtc aattcctatt
     1681 ggagtggttt caggtcttcc agaataccat tttgatccta acgctatttt tagattgcct
     1741 atatcataag ttccagccca agacaccaca ttattcaatt caaaattgtt cgaaaattct
     1801 gtttgttcaa tacctggaaa gtgataattg ttgtcattaa aggcatagct caaccaagtg
     1861 ataaaatgat ttattttttt ttgaaccagt atttcagaac caaaaacttc atagtcgcca
     1921 tttattttgg caaactccaa ttggttttga aacccttgac ccgaactatt aattccggtt
     1981 acttttttat aaaaattatc taaagtaatc aaccaattgt tcttgttgta agaaactcca
     2041 atcgaggctt gtttgctttt ctggatgggc actgtactgt tgtttgatag gatccaacgt
     2101 cttttttcga caccaaaata atcgccttga agatctataa cctgaaagca gttttggctt
     2161 ttaaattctc ccaatatttc caaactcaag ctgtttgaaa tgccataatt aaactgaaat
     2221 ctcggctcaa ataaatattt tttgaatttt tcaatataat ttagacgcat tccaacattg
     2281 aaaaatattt tagaaagggt atccttgtat tttccttctg ctatgagtgc gtgcgtgcgc
     2341 aaaacttctt tgatttttct ataaaaagcg ggcgtattga cttggtcgag atttgtagag
     2401 ccaatttcgt taaactggta gccattatta aaagaaaatt tggaagtgat gctgtggttg
     2461 ttttcgattt ttagaccatt gtctaatagt gtgttttctt gttttacaat ttggttgtcc
     2521 tgaattttat ttttctctgc atctagctcg tatgaggaac taaaaatgct gattttacta
     2581 ctattgttag tgttccagtt tcgtttccac aaaagatttg ctccgtgatt tttttgatat
     2641 aaacggttgt tttcagattg aacaatttca ttaatcgttg ctttttgatt cacatcgagt
     2701 tggtcattaa tggcaatgag atctaaaatt agatggtctt tttctcctat tttttgggaa
     2761 tatttaagcg taatgtcata aaaattaaac ttctcatcat tctggtaatc tatattttgt
     2821 ttggtggcaa aatcagaaac ggttgtgttt tgaaaagcct tgttatagta tttggtataa
     2881 gtgggagttt ttactaaatc agtaatagat tttcttgcag caatttctaa aaaaccattt
     2941 ttattgatgt taaatttaga ataaatatca gcattaatca tattaattcc agcactaaat
     3001 tgtggttttt cgaacttatt tggatcagaa gaaatatcca ctacactcga aacactttcg
     3061 ccataaaaag ctgaacttcc attttttgaa atagaaaccg tactaggcaa attaggattg
     3121 aatgccgata ttaaaccaaa aaaatgtccc gtttggtaca tttttattcc attccataag
     3181 aataggtttt ggtcgtgagt tccacctcta acattgatgc ttgataaact ctcgtcagcg
     3241 gtgtagattc ccggaatttg tagcatagtt tgcaatacgt cgggttcaat taagccgggt
     3301 aatattccaa gctttttggg ttttaccaca aatgttcctt ctgtagtttt tgaaattcca
     3361 gaagctaaat aatggttggc catcacattg tcaagttcta acacatcaag ttccagaaaa
     3421 attttgagac aatttttatt ctttaaattg gctacagcaa ttttttgaga tctaaaacca
     3481 acatggctta tttctatttc agttgcattt tcttttttaa tttcaaaata gcccttttta
     3541 tcggtggtag tatgactgcc atttaggatt tgaatgttgg cactttcttg gggtaagttg
     3601 tcatttttcg aaaatacgta accacaaatg agttcgacct ctttttttga agaaatcgtg
     3661 atgaactgtg aatcgacttt atcgaataaa agattggttt ttttttcgag atagacaatc
     3721 ttatcattca atgataatga tcttttgggt ggttcaattt taataacaac aatttcgttt
     3781 tctatataat tgaaataaac atggtgttgt ttttcaatag attgcaggat tgtttttaat
     3841 gcaattgctt tgcttccgtc ctgagctttt agattcaagg caaaaacaaa gcaaaaaaac
     3901 aaaacacaat tacgttttat aaaaaacatt tattttcctt caaaaattat tttatttggg
     3961 ctaattttat ttggttctaa atgatacgtt gttgcaataa tttctagagc gacatcaagg
     4021 ttgttgagcg ggatttttcc ggtaaacaat tcacttggat aatctgcttg aacctcgata
     4081 gttaggttgt actgtctttg aatttcatct aatatgttgc gtaaattttc tttactaaaa
     4141 gcaattttgt tttctagcca ttctggtttt tgattgtcca ccaacaagtt gatttgtttc
     4201 ccgttttcga aagccacact ttttccgggt gtcaaaataa tttctttgtc tttgtaattg
     4261 acttttactc ttccttcaaa acaagtaatg tcaaaacgat tttttctggc ttttacattg
     4321 aattgagttc caagaactgt tacttttcct agagtagtct ttacttcaaa tttttcacct
     4381 tttgctactt taaagtaggc ttcacctttt aattgcaggt ttctgttatt gtcccaattc
     4441 cattttttat attcaatttc tgaaccagcg ttcaaaacta cttctgaatg atcaggaagc
     4501 gaaaaagtag ttctagctcc attgagcgcc aattgagttt cactcgaaaa attttgaacc
     4561 gtaaatgtta ttcctaatgc tatcactaaa attgcagcca ctctaaaaat ccaatttttg
     4621 tacaacggaa tcactttagg tgttgttttt ttatgctgaa gaatgttttc taaaactttt
     4681 gcttcatcaa aatcggtgac ttttagttga gcagaataat tttttatttt ttcgtatttt
     4741 tcaaaatcag gatcggcttt aaattctgct aattccgcat cagtcaattc atcattgagc
     4801 catttcgtta agatgtaatt tttttccata tttttaatct tgttatgtat agaacaagca
     4861 acactgtttt taccctactt aaaatcaatt gtttttctta attcgactaa tgccaagtgt
     4921 atccgttttt cgactgcttt aacactaata tttaattctt cggcaatttc actgtatttt
     4981 ttttggtcaa ttcggtgcat caaaaaagcg actctttggg tctcgtttaa gtcttcaatt
     5041 gcttttagaa gcttattttt aaactgcttt tcttctaaaa taaattcagg gttttcattg
     5101 gttttatccg ccccagtaaa attattctca tactccaaaa ctactttttt atgcttaatt
     5161 tcgtttaacg aactattgtt ggcaatggtg taaatataag atttagcttt ttcgacagga
     5221 acagatgtgc agtttt
//
LOCUS       DAOSNN010000193         5227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_15943_length_5227_cov_3.982019, whole genome shotgun sequence.
ACCESSION   DAOSNN010000193 DAOSNN010000000
VERSION     DAOSNN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15943_length_5227_cov_3.982019"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PLQ70_09600"
     CDS             <1..930
                     /locus_tag="PLQ70_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQE34907.1"
                     /translation="LIDIAPKDFWGAQDTHLEMIKKYYPKLKIVARGTTLKAFGEKEV
                     LDEFENRFNRLMLHFTRYNNIDDNVIERVLQSTVQEDAKAFGHDKILVHGVGGKIIKA
                     MTPNQQLLVDTMSKNDMVFAVGPAGTGKTYTGVAMAVKALKEKQVKRIILTRPAVEAG
                     ENLGFLPGDMKEKLDPYMQPLYDALRDMLPNEKLEEHITKGIIQIAPLAFMRGRTLDN
                     AFVILDEAQNTTHSQMKMFLTRMGKNAKFMITGDPGQVDLPRRTISGLKEAILVLKDV
                     EGIGIIYLDDKDIVRHRLVKKVIDAYKQIENHD"
     gene            1447..2262
                     /locus_tag="PLQ70_09605"
     CDS             1447..2262
                     /locus_tag="PLQ70_09605"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HQE34908.1"
                     /translation="MTADFSKIKVIVSDLDGTLLNNKHQISEYTKAVFQQLHNENYLI
                     IVATGRHHLDALPIVEDLGCPAYLVTSNGARIHSPTKELIYSFDIKSDAIKSILDMDI
                     DPEFTTVLFKEKAWQTNKENKKLNSFQTVMNYPFEIVDYSVMEDLSAIKLFFTHDNHY
                     KLVELKDKIMKVHSEEFHYAFSLPFCLEFMDKSVDKSVAISKILEIENYTFEESIAFG
                     DGFNDEKMLISSGKSLIMENAVESLKSKLKHLEVINSNDNEGVAKYLSANLFS"
     gene            2384..3340
                     /locus_tag="PLQ70_09610"
     CDS             2384..3340
                     /locus_tag="PLQ70_09610"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQE34909.1"
                     /translation="MSNTITTTNFNFPNQKSVYRGKVREVYNINDELLVMVATDRLSA
                     FDVVLPKGIPYKGQILNQIATKFMELTQDIVPNWLVATPDPSVAVGHLCTPFKVEMVI
                     RGYVSGHAAREYAAGRRQVCGVTMAEGLKENDKFSEPIITPTTKADNGSHDEDISRED
                     ILSKGIVSEEDYLVLEKYTRALFQRGTEIAASRGLILVDTKYEFGKTKDGVIVLIDEI
                     HTPDSSRYFYSEGYQERQDRGEEQKQLSKEFVRRWLIENGFQGQEGQQIPNMTDEYIE
                     TVSDRYIELYENILGEKFVKADISNINERIERNVLGYLESLK"
     gene            complement(3475..4800)
                     /locus_tag="PLQ70_09615"
     CDS             complement(3475..4800)
                     /locus_tag="PLQ70_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQE34910.1"
                     /translation="MRKIILSVILITILVLSVFSIYLFPESTVLGAHVVELKLDTGDT
                     AWMLTATGLVLLMTPGLGFFYGGMVGKKNVISTVLQSFIAMVIVTVLWVIIAFGLCFG
                     PSIGGFIGDPSANLLFNGVNSTTAWELAPTIPFILFALFQAKFAIITPALITGAFAER
                     VRFWAYILFMVLFILFIYAPLCHMTWHPDGLFFGWGVLDFAGGTVVHMSAGWAALAGA
                     IFLGKRKTQKTNPARITYVLLGTGLLWFGWFGFNAGSAVGSNGLAAQALGTTTVAAGA
                     AAMAWVFLDKILGHKLSAMGACIGAVVGLVAITPAAGFVTIPSALAIGIIASIVSNLV
                     CSKFPKGKIDDALDVFACHGVGGMVGMLLTGVFASKSVNAIVGDNQGLIYGDATLFLI
                     QLKALVIVSIFAFCASYALFFIVNKITPLRVSEEKEELGLDISQHGEYL"
     gene            complement(4992..>5227)
                     /locus_tag="PLQ70_09620"
     CDS             complement(4992..>5227)
                     /locus_tag="PLQ70_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HQE34911.1"
                     /translation="HNLQYIDTHFKNRTKFTFHPSYPIIYFDDELISKNLFDNKIITT
                     SFKYTNASGKLNRIVITSNHIVTDLDQLIAQLY"
BASE COUNT         1801 a          821 c          947 g         1658 t
ORIGIN      
        1 ctcatagaca tcgcaccaaa ggatttttgg ggcgctcaag acactcatct tgaaatgatt
       61 aaaaagtatt atcccaaatt gaaaattgta gcccgaggaa cgactttgaa ggcttttggc
      121 gaaaaagaag ttttggatga gtttgaaaac cgcttcaacc gcttgatgct ccattttacg
      181 cgttacaaca acatcgatga taacgtgatt gaacgtgtgc ttcaaagtac ggtgcaggaa
      241 gatgcaaaag cctttggtca cgacaaaatt ttggtacacg gagttggcgg aaaaatcatc
      301 aaagcgatga cgcccaatca gcaattattg gtggatacta tgtccaaaaa tgatatggtt
      361 tttgccgttg gtccagcagg aacagggaag acttacactg gagttgctat ggccgtaaag
      421 gcgttgaaag aaaagcaagt caaacgaatt attttaactc gcccagcggt tgaagcagga
      481 gagaatcttg gttttcttcc aggagatatg aaggaaaaac tggatccgta tatgcagcct
      541 ttatacgatg ctttgcggga tatgttgccc aacgaaaagc tagaagaaca tatcacaaaa
      601 ggaattatac agattgcacc attagccttt atgcgtggta gaacgttgga taatgccttt
      661 gtaattctgg acgaagccca aaataccacg cattcgcaaa tgaaaatgtt cttgactcgt
      721 atgggaaaaa atgccaagtt tatgattact ggcgatcccg gtcaagtcga tttgccaaga
      781 cgcacaattt caggattgaa agaggctatt ttggtcttga aagatgtgga aggaatcggg
      841 attatttatc tggatgacaa agatatcgtt cgacatagat tggtcaaaaa agtgattgat
      901 gcgtataagc aaattgagaa tcacgattaa atttatgagg tataaagggt atatttttgt
      961 tgcgatcttt tttatagtat tgatttcgat attttataat attgattttt acagttctat
     1021 aataccagga tggcatacaa caatatatcc tttttgggaa gtagtttttt tggctttcgg
     1081 tattgctaat tatggttgta ttggcaattg taattttctt gaaattaata aaaatcttaa
     1141 ttagttttta gtttttgttt tttttttttt tcaatattat taataacggc tgagcagtta
     1201 tatatttata gaacgataaa tatttataga aattgaattg atttgtaatg atattcgacc
     1261 ccagctgggg tcgaatccaa taattgattt tgttttttta taaatatgaa atcccgctgg
     1321 gattttttgg tcaattaatt ctttcgttaa atactgaatc tctatgggat tttaaagagg
     1381 atcttagaaa tgattttttt tcctccaagt ataaatcctt aaatttgccc tacacaatta
     1441 tttgatatga ctgcagattt ttccaaaata aaagtaatag taagcgattt agacggaaca
     1501 ttgctcaaca ataaacatca aatttcagaa tatacaaagg ctgtttttca gcaacttcac
     1561 aacgaaaact atctcattat tgtggctaca gggcgtcatc atctggatgc gttgccaata
     1621 gttgaagatt taggttgtcc cgcttattta gtgacttcca atggagcgag aattcattcg
     1681 cccacaaaag aactaattta ttcttttgac attaaaagtg atgccataaa atcgattttg
     1741 gatatggata tcgacccaga gtttactacc gttttgttta aagaaaaagc ttggcaaacc
     1801 aataaggaaa ataaaaagtt aaacagtttt caaaccgtga tgaattatcc gtttgaaatt
     1861 gtagattaca gtgttatgga agatttgagt gccataaaat tattttttac tcacgacaat
     1921 cattataaat tggtagagtt aaaggataaa attatgaaag tacattcaga ggaatttcat
     1981 tatgctttta gtcttccgtt ttgtttggaa tttatggata aatcggtaga taaaagtgtc
     2041 gctatttcta aaattctaga aatagaaaac tatactttcg aagaatctat tgcttttgga
     2101 gatggtttta atgacgaaaa aatgcttatt tcttcaggga aatccttgat tatggaaaat
     2161 gcagtcgaaa gtttaaaaag taaattaaag catttagaag tgattaattc taatgataac
     2221 gaaggtgttg ctaaatattt gtcagctaat ttgttttctt aatttgattg aagtgtttat
     2281 tttaaaacct caaatgactt tgaaatagaa ttttgggttt tctttgtgtt ctctattaat
     2341 tagttttaaa tttgcattca taaaaaacaa caacacaaaa ccaatgagta atacaattac
     2401 cactaccaat ttcaattttc cgaatcagaa atcagtttac cgaggaaaag ttagagaagt
     2461 ttacaatatc aacgacgaac ttttggttat ggttgctacc gataggcttt cggcttttga
     2521 cgtggtttta ccaaaaggaa ttccctataa aggacaaatt ctgaatcaaa ttgccaccaa
     2581 atttatggaa ttaacccaag atattgttcc caattggtta gttgccactc ccgatccaag
     2641 tgtagctgtt ggccatttgt gtactccttt caaagtagaa atggttattc gtggttatgt
     2701 ttcgggtcac gctgcacgcg aatatgccgc aggaagaaga caagtttgtg gcgtaacaat
     2761 ggctgaaggt ttgaaagaaa acgataaatt ttcagaaccg attataactc caactactaa
     2821 agccgacaat ggttctcacg acgaagatat ttctcgtgaa gatattttat caaaaggaat
     2881 tgttagcgaa gaagattatt tggttttaga aaaatatacc agagctttat ttcaacgagg
     2941 aactgaaatt gctgccagtc gaggattgat tttagtagat acaaaatatg aatttggaaa
     3001 aaccaaagac ggagtaattg ttcttattga cgaaattcat actccagatt cttctcgtta
     3061 tttctattct gaagggtatc aagaaagaca agatagaggt gaagaacaaa aacaattatc
     3121 taaagaattt gtgcgtcgtt ggttaatcga aaatggtttt caaggacaag aagggcaaca
     3181 aattccgaat atgaccgatg aatatattga aactgtttcg gatagatata tcgagttata
     3241 cgaaaacatt ctaggtgaaa aatttgtaaa agctgacatt tccaatatta acgaacgtat
     3301 agagagaaat gttttagggt atttggaaag tttgaaataa cttttaagag tatttgtgag
     3361 aatcgcaatt catttatttg gattgcgatt ttttttgtta gaaagatttt taagcaaaaa
     3421 aaacactcta aaaactaatt tctagagtgt ttcaaaaatt aaataaatgg tattttatag
     3481 gtactctccg tgttgagaga tgtctaatcc taattcttct ttctcttcag aaactcttag
     3541 aggagttatt ttattcacaa taaagaataa agcataagaa gcacagaaag caaatattga
     3601 aacgattact aatgctttta attggattaa gaacaaagta gcatcaccgt agattaaacc
     3661 ttgattgtct cctacaatag cgtttactga ttttgaagca aatacaccag ttaatagcat
     3721 accaaccata ccaccaacac cgtgacaagc aaatacatct agggcatcat cgatttttcc
     3781 tttagggaat ttgctgcaaa caagattact cacaatacta gcaatgattc cgatggcaag
     3841 tgcagaagga attgtaacaa atccagcagc aggtgtaatg gcaactaaac caacaacagc
     3901 tccgatacaa gctcccattg cagataattt gtgacccaaa attttgtcta agaaaaccca
     3961 agccattgca gcagcaccag cagctactgt agtagttcct aatgcttgag cagcaagtcc
     4021 gttagatcca acagcagaac ctgcattgaa accaaaccaa ccaaaccata ataaaccggt
     4081 tcctaataat acataagtaa tacgtgctgg gtttgttttt tgtgtttttc tttttcctaa
     4141 gaaaatagct cctgcaagag cagcccaacc agcactcata tgtactacag ttccaccagc
     4201 gaaatctaat actccccatc cgaagaataa tccatcagga tgccaagtca tgtgacataa
     4261 tggtgcgtag ataaataaga taaacaatac cataaacaag atataagccc agaaacgaac
     4321 acgctctgca aatgctcctg taattaacgc aggagtgatg atagcaaatt tcgcttgaaa
     4381 taatgcaaaa agaataaatg gaattgttgg tgctaattcc caagctgtgg ttgaatttac
     4441 tccattgaaa agtaaatttg cggatggatc accaataaag ccacctatag atggtccaaa
     4501 acacaatccg aatgcaatga ttacccaaag aacagttaca ataaccatag ctataaagct
     4561 ttgaagtact gtgctaatta cgttcttttt acctaccata ccaccataaa aaaatcctag
     4621 acctggagtc attaatagta caagtcctgt agctgttaac atccaagcag tatctcctgt
     4681 gtcaagtttt agttctacta cgtgagctcc taatacagta gattcaggaa acaaataaat
     4741 cgaaaacact gataaaacca gaattgtgat taatatcaca cttaaaataa tctttcgcat
     4801 aattttagtt tttttagtta taaatttcga taaaagtata aaatcatttc atacccccat
     4861 aaaaaaactt gttttaagta tgatttttgt caaatttgta ttttaccccc ctaaatttta
     4921 tgggtgaaag ataaattatt gtcaaaattt acaatttgtt aatgctctta gaagtaaatt
     4981 ttaaggttta attaatataa ttgagctatc aattggtcta aatctgttac tatatgattg
     5041 gatgtaataa caattcgatt cagtttgccc gaagcatttg tatatttgaa agaagtagtg
     5101 atgattttat tgtcaaataa attttttgaa atcaattcat catcaaaata gataattgga
     5161 taggagggat ggaaagtgaa ttttgttcta tttttaaaat gggtatctat atactgtaga
     5221 ttatgtt
//
LOCUS       DAOSNN010000194         5202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_16050_length_5202_cov_4.931805, whole genome shotgun sequence.
ACCESSION   DAOSNN010000194 DAOSNN010000000
VERSION     DAOSNN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16050_length_5202_cov_4.931805"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PLQ70_09625"
     CDS             <1..1105
                     /locus_tag="PLQ70_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006266451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQE34912.1"
                     /translation="QFEEPLFHIHLNVDYPFNLTGILYFPKLGSDLQIQKDKIQLYQN
                     QVYVTDNVEGIVPEFLTMLKGVIDSPDIPLNVSRSGLQADGAVKKISNYITRKVADKL
                     KSLFTENREDFEQKWNDIKIVLEYGMLSEDKFYEKAGAFVLYPTVDDKYFTLEELKEN
                     LKENQTDKDGKLVVLYAGNKEAQHSYIEIAKEKGYEVLLLDSPIISHLIQKIEGDNKD
                     LTFVRVDSDHIDNLIKKEETTISKLSDTEKETLKASLETYIPKAYTVQLEALDSNAAP
                     FIITQPEFMRRMKEMSQSGGGGMFGMGNMPEMYNLVVNTNSDLASNILNTEDKTTQES
                     LVKQALDLAKLSQNLLKGEELTAFVKRSFDLIK"
     gene            1476..1967
                     /locus_tag="PLQ70_09630"
     CDS             1476..1967
                     /locus_tag="PLQ70_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34913.1"
                     /translation="MKKKNSFLLIVYVISFITTWLCFIFWSDKNEFDLEFSEKTTTLA
                     TIFNIGTDEVVEELYDGRKTIVHNVNYVEYFYSVNGKKYKYGSKYYGENYSISDKIKI
                     EYLENNPAISKIKGQNEYSFNYFIRNLIPVSIISLIIMFCFFGILDFFPKLKILDNLY
                     DKQ"
     gene            2130..2846
                     /locus_tag="PLQ70_09635"
     CDS             2130..2846
                     /locus_tag="PLQ70_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34914.1"
                     /translation="MKKIKKKKLINISVLLCVIAFILPFFFKKSNDSLLSTISVSFTA
                     IGATATLITLFIAIFLYERFGLESRFINNQTDKVLELVDQLKGKSIRCETNNGTYLLG
                     TNRDKLKFVKEFPKFNEIDKEKIVLITLEDYKNSWDKVLEISRSYWLPQKIKEKISFL
                     NLIMVTNVENPLNNEYVRLKFDTENENEWVEILPKLTLLEFINHLDNLSKSIEKWLKQ
                     HSDITIDFKLEEPEKYPSEK"
     gene            3179..3556
                     /locus_tag="PLQ70_09640"
     CDS             3179..3556
                     /locus_tag="PLQ70_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34915.1"
                     /translation="MHVILIILPLLFQIIFGRKAIGGDIKLSFGTICLISFLGQILFT
                     ILAFNLIAYYLEKNNNACGMPLVGLVMFSLLFTLILLITVFIQYRIKKSYGDDDEIED
                     EDEDENDKIDDEQDDEDEEYEIN"
     gene            3737..4141
                     /locus_tag="PLQ70_09645"
     CDS             3737..4141
                     /locus_tag="PLQ70_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020213604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HQE34916.1"
                     /translation="MTVHIETPALLFSATSLILLAYTNRFLTIATIIRGLKKAYKEKE
                     SSMVLLEIKNLNLRLTLIRYMQMAGVLSLFLSVFTMLLLFLDEQEFGVKLFGLSLLSL
                     LISLGLSFWEINISVGALRVHLSDLMEKKEAK"
     gene            4374..>5202
                     /locus_tag="PLQ70_09650"
     CDS             4374..>5202
                     /locus_tag="PLQ70_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HQE34917.1"
                     /translation="MDKNTLWSLRLGYSAKQAKTIEKIGLKKFLEQSFATKFDSKIPD
                     FLEDSPKSIKELQKAKKALMSENPEAKKELLKKEIQTSAEMRAWWIDKMNQDEFPLRE
                     KMTCFWHNHYVSTFQKVKVNYWVFQHNQILRENAFGNYKTLTKKIIQSNAMVRYLDNV
                     DNRKGKLNENLSRELLELFTLGIGNYSEDDIKNGAKGLAGLGVGEENAVYRRIFEDND
                     NFVYLGKKGNFKIDEMIDIIFEQPNIPYLITRKILKWFIYDNPKEELVRYYGDYFKKM
                     "
BASE COUNT         1978 a          785 c          864 g         1575 t
ORIGIN      
        1 gcaatttgaa gaaccgttgt tccacattca tttgaacgtg gattatccgt tcaacctgac
       61 tggtattttg tattttccaa aattgggttc tgatttgcaa atccagaaag acaaaatcca
      121 attgtaccaa aaccaggttt acgttaccga caacgtggaa ggaatcgtgc ctgaattctt
      181 gacaatgttg aaaggtgtga ttgattcacc ggacattcca ttgaatgttt ctcgttcagg
      241 cttgcaagct gatggcgcgg tgaagaaaat ctcgaactac atcactcgta aagttgccga
      301 taaattgaaa tcgctgttta ccgaaaatcg tgaagatttc gaacaaaaat ggaacgacat
      361 caaaatcgtt ttggaatacg gaatgctttc agaagataaa ttctacgaaa aagcaggtgc
      421 ttttgttttg tacccaaccg tggatgacaa atacttcaca ttggaagaat tgaaagaaaa
      481 cttgaaggaa aaccaaaccg acaaagacgg aaaactggtg gttctttacg ctggaaacaa
      541 agaagcgcaa cactcttata tcgaaatcgc caaagaaaaa ggatacgaag tattgctttt
      601 ggattcgcca atcatctcgc atttaatcca aaaaattgaa ggcgacaaca aagatttgac
      661 tttcgttcgt gtcgattcag accatatcga taatttaatc aagaaagagg aaactaccat
      721 ttctaaatta tcggataccg aaaaagaaac cttgaaagct tcattagaaa cttatatccc
      781 aaaagcctac accgtgcaat tggaagcttt ggacagcaat gctgctccgt tcatcatcac
      841 gcaaccagaa tttatgcgtc gtatgaaaga aatgagccaa tctggtggtg gcggaatgtt
      901 tggaatggga aatatgccgg aaatgtacaa cttggtggta aacaccaact cggatttggc
      961 ttccaatatc ttgaacactg aagacaaaac aacccaggaa agtttggtaa aacaagcttt
     1021 ggacttggcc aaattatccc aaaacctgtt gaaaggcgaa gaattgacgg ctttcgtaaa
     1081 gagaagtttt gatttgataa agtaagccga gaacctattt tatttaataa aatggagtga
     1141 atcgcttatt ccgaacttgg ttcggaatct tttcgaaaaa tagaaaattc aaaacctgca
     1201 agtgaaaatt tgcaggtttt tttgttttgg agcagaacaa atgttttcaa acagaccata
     1261 atcatcaatc aaatagactt tttcaaagta caatttcttt ttaagttaaa acttatttga
     1321 tgatttgtaa ataaatattt atatatttga tgaaataatg ctacgaataa atgtacccaa
     1381 aaacatacgc actacataac aatttagaac aaaaactggc aattttatgc gtataagttg
     1441 gcagacagtt gtagaaaaat acagaaaatc aaaatatgaa aaagaaaaat agttttttat
     1501 taattgttta cgtaatttct tttataacaa cttggttatg ctttattttt tggtctgaca
     1561 aaaatgaatt tgatttggag tttagcgaaa aaacaacaac tttagcaaca atatttaata
     1621 ttggaactga tgaagttgtt gaggaattat atgatggtag aaaaaccatt gtccataacg
     1681 ttaattatgt agaatatttt tattctgtaa atggaaaaaa atacaaatac ggaagcaaat
     1741 attatggaga aaattactct atttcagata aaattaaaat agagtatttg gaaaacaatc
     1801 ctgcaattag caaaattaaa ggacaaaatg aatatagttt taattatttt attcgaaact
     1861 taatacctgt gagtattatt tctctgataa ttatgttttg tttttttgga attttagatt
     1921 tttttccaaa attaaaaata ttagataatt tgtatgataa acaataaatt ataagaaata
     1981 aatacaacta aaaatagaat tcattcctta aaaaatttaa aatcaaactg cttcaattaa
     2041 acggaataga aaattggaat tgacaaacat tataaatatt caaccgatct accaacaggt
     2101 acaacagatt tgggtaaaaa aaataaacta tgaaaaaaat aaaaaagaag aaacttataa
     2161 atatcagtgt tttactttgt gtaattgcat ttattcttcc atttttcttt aaaaaatcta
     2221 atgacagttt attatctaca atttccgttt cctttacagc aattggtgct acagcaactt
     2281 taataactct atttattgca atctttcttt acgagagatt tggtttggaa tcaagattta
     2341 ttaataatca aacagataaa gttctagaat tagtagacca actaaaaggt aaatctatta
     2401 gatgtgaaac taacaacgga acgtatttat taggcacaaa tagagacaaa ttaaaatttg
     2461 tcaaagaatt tccaaaattt aatgaaattg ataaagaaaa aatagtttta ataactttag
     2521 aagattataa aaattcttgg gataaagtat tagaaattag cagaagttat tggcttccac
     2581 aaaaaataaa agaaaaaatt tcatttctca atttgattat ggtaactaat gttgagaatc
     2641 ctttaaataa cgaatatgtt agattaaaat ttgacacaga aaatgaaaat gaatgggtag
     2701 aaattttgcc aaaacttaca cttttagaat ttattaatca tttggataat ttatctaaat
     2761 caatagaaaa gtggcttaaa cagcattcag acattacgat tgattttaaa ttagaagaac
     2821 cagaaaaata tccatcagaa aaataaagca catctgctaa caaacgctac aagttcccca
     2881 attccccctg tgctggcgcg agcgtcccgc tcgtgcacac aaacaaaata aaaaataaag
     2941 aaattacaaa ttgaaatggg tactagcggg acgctcgcac tagctgtgag aaaattacaa
     3001 atagaaacta ataaataaat gcacgtaatt ttaattatac taccccctgt tagcgcgagc
     3061 gtcccgctcg tgcacacaaa caaaataaaa aataaaggaa ttacaaattg aaatggttac
     3121 gattggaact ctcttactag ctctgagaaa attacaaata gaaactaata ataaataaat
     3181 gcacgtaatt ttaattatac tacctctatt atttcaaatt atttttggca gaaaagctat
     3241 tggaggagat attaaattga gctttggaac aatttgttta attagttttt tgggccaaat
     3301 cctattcacc attttggcgt ttaacctaat tgcatattat cttgaaaaaa acaataatgc
     3361 ttgtggaatg ccattagttg gacttgtaat gttttcgtta ttatttactt taatcttatt
     3421 gataacagtg tttattcaat accgcattaa aaagtcatac ggagatgatg atgaaattga
     3481 agacgaagat gaagacgaaa atgataaaat agatgacgaa caagatgatg aagatgaaga
     3541 atacgaaatt aattgaaaga cgaagaactt actgctttcg taaatagaag ttttgatttg
     3601 attaaatagt atctagaaaa aagtattaac tagtatgacc tgcaagtgaa aatttgcagg
     3661 tctttttttg ttcaaaaaaa aaccacatat tgaagaaaga atcccgaact ttgaaagtaa
     3721 attaaatatt taaaaaatga ctgtacacat tgaaaccccg gctctacttt tctcggctac
     3781 ttcattaatt ttactcgctt acaccaatcg atttcttacg attgccacca ttattcgagg
     3841 attaaaaaaa gcttataagg aaaaagaaag cagtatggtt ttattagaaa taaaaaacct
     3901 caacctgcgt ttaacactca ttcgttacat gcaaatggcg ggtgtcttga gtttatttct
     3961 atccgtattc acgatgttgc ttttgttttt ggatgaacaa gaatttggag ttaaactttt
     4021 tgggttaagc ctacttagtt tgcttatttc attgggattg tcgttttggg aaatcaatat
     4081 ttctgttggt gcacttcgag tgcatttgag cgatttgatg gagaaaaaag aagcgaaata
     4141 aaacaaaact ctttgaggac taaactaaaa aatggcacac ggataacacg gattgaacgg
     4201 ataaaaactg atttttttta attttaattc gtgataatca gctaaaatcc gtttaatcag
     4261 cgtaccatta ttttattgac ataagtaacg gatagccata aaatcaaaag atttcataaa
     4321 gcgattaaac ccttttataa aatctaagtc caacttttat agcactttag attatggaca
     4381 aaaacacact ttggtcattg cgcctcggat attcggcaaa acaagcaaaa actatcgaaa
     4441 aaataggttt gaaaaagttc ttggaacaat cttttgccac caaatttgat tctaaaattc
     4501 ccgattttct ggaagacagt cccaaatcga ttaaagaatt acaaaaagct aaaaaagctt
     4561 taatgtccga aaatcccgaa gccaaaaaag aattactcaa aaaagaaatt cagacttctg
     4621 ccgaaatgag agcgtggtgg attgacaaaa tgaaccaaga tgaatttccg cttcgcgaaa
     4681 aaatgacttg tttctggcac aatcattatg tttctacttt ccagaaagta aaagtcaatt
     4741 attgggtttt tcagcacaat caaattctga gagaaaatgc ttttggcaat tataaaacct
     4801 tgaccaaaaa aatcatccaa agcaatgcaa tggtgcgtta tttggacaat gttgacaatc
     4861 gaaaaggcaa actcaacgaa aacctgagtc gagaattgct tgaacttttc acccttggca
     4921 tcggaaatta ttccgaagac gacattaaaa atggagccaa aggtctagct ggcttgggtg
     4981 ttggcgaaga aaatgctgtt taccgaagaa ttttcgagga caacgacaac ttcgtttatc
     5041 ttggcaaaaa aggaaatttc aaaatcgatg aaatgattga catcattttc gagcaaccca
     5101 acattccgta tcttattacc agaaaaatat tgaaatggtt catttacgac aatccaaaag
     5161 aagaattggt tcgttattat ggcgattatt tcaagaaaat ga
//
LOCUS       DAOSNN010000195         5199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_16068_length_5199_cov_4.678655, whole genome shotgun sequence.
ACCESSION   DAOSNN010000195 DAOSNN010000000
VERSION     DAOSNN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16068_length_5199_cov_4.678655"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLQ70_09655"
     CDS             complement(<1..253)
                     /locus_tag="PLQ70_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019387566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34918.1"
                     /translation="MKLPIIKHLTQFIDENDQDYLVETIEVLEALTEVSSLKDEELDV
                     IGELISNMYGALEVHKMTKDGMDKKEALNTFMKRVLGSID"
     gene            complement(256..564)
                     /locus_tag="PLQ70_09660"
     CDS             complement(256..564)
                     /locus_tag="PLQ70_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014084922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQE34919.1"
                     /translation="MLIELNEDTLQDLVAKEEKVIVQFSASWCGNCRIMKPKFKKLAS
                     ENDDLTFVLVDAENSPESRKLANVSNLPTFATFVNGKLVNETQTNKAEVLTELVNEIK
                     "
     gene            complement(738..1373)
                     /locus_tag="PLQ70_09665"
     CDS             complement(738..1373)
                     /locus_tag="PLQ70_09665"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQE34920.1"
                     /translation="MSLVGKKFPNITVDAISEMGDNLRINVLEEATENNQKVLLFWYP
                     KDFTFVCPTELHAFQAALGEFKKRNTIVIGASCDTNEVHFAWLNTPKNSGGIEGVTYP
                     ILADTTRNLAAELGILDIEMEYDEAFDGELLVGSNVPYRATYLIDEEGKIFHESVNDM
                     PLGRNVNEYLRLVDAYTHVQTKGEVCPANWEEGKDAMTADRNSTAAYLGSH"
     gene            complement(1520..3118)
                     /locus_tag="PLQ70_09670"
     CDS             complement(1520..3118)
                     /locus_tag="PLQ70_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQE34921.1"
                     /translation="MTLEQKIGQFFFPAVFINDTEENFQEIERLITEHNIGGLTFFHS
                     RASAATNYESKEKVEVNDSSFERLKELVVRFQKCASTPLLMSIDAEWGLAMRVENTPQ
                     YPYAISLGALPERYEYLVYEVGRRTGLDLKAAGIHYNLAPLADINNNPKNPVIGYRSF
                     GQNKNRVANFSTEYLRGLNEVGVLGCLKHFPGHGNTSVDSHLGLPILEETLEQLMENE
                     LVPFIKGIENNVDSIMIGHLAVPALNEGKNTSATLSKSIIEDLLRKQLKYDGLVISDA
                     LNMHSVSKLYDIKGQLEWEAFNAGNDVLCFAENVAEGIQEILKNATPKRIEESFNRLW
                     KCKQKAGILDGNFAPEGEFDFEATSILNRLIADKSTTLIKDKQNGLHLFEAKNNDKLA
                     KLCLYKNIDNIFYKSLKLVLPSEKFALETGDKTAIANIKEELKCFDTILVTLFVPKAK
                     PLNNFGIEDSVLEFLEDLFSKKKCVLYVFGNPYVLDVIPNLKSTIGIVQVYQDFREFQ
                     ESAARQLLRNSRCRGSLPVTINGF"
     gene            complement(3338..4615)
                     /locus_tag="PLQ70_09675"
     CDS             complement(3338..4615)
                     /locus_tag="PLQ70_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010520168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE34922.1"
                     /translation="METSKTKNEPWYWIPFLNFASGLPYAIIISVSVIMYKSLGISNE
                     DIGIYTSLLYLPWVIKPLWSPFIDLFSTKRNWFLVMQLLISIAFLVVGFTIPVSNFFI
                     LSLAIFWVAAFASASNDVASDGFYMLALTKDQQSFFLGIRSTFYRLAMLTGNGLIVII
                     AGYFEQLYADKTKAWSYTMIIVGLIMTALTIYNYFLTPKTEINSLETESQPTPKVSFG
                     TVFSSFFQKKQIGIALSFILLFRLGESQLLKMLTPFLIDDKAVGGMGLLTEDVGIIYG
                     TFGVLALTIGGILGGIAISKNGLGKWMLPMILTMHLPIIGFILLAHFHPTSIYYIYAV
                     VIAEQFGYGFGFASFMMYLIYLADGESKTSHYSIATGFMALGMMLPGMLSGYIQEYLG
                     YGNFFIWVFLATIPGLILSQFLNFPKEFGKKTN"
     gene            complement(4617..>5199)
                     /locus_tag="PLQ70_09680"
     CDS             complement(4617..>5199)
                     /locus_tag="PLQ70_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQE34923.1"
                     /translation="WANNSINANVYIGNGSYKIKNDSDKKWFVPGEIPNQIDLTRTFP
                     NIQGNSFYSAKAFVNINKDVADLLKANQYKYPAIPLPVPNSKKTGTERPLINSFSKEG
                     NLYQFAFKKTQSDQVRYIVVYITENNTKLDSNDPSQILEKIFIDAKSESIKYSIAKDQ
                     LNNIKKVAFTFIDFYGNESVPEIVNLNTEIKQN"
BASE COUNT         1659 a          883 c          798 g         1859 t
ORIGIN      
        1 tatcaattga gcccaaaacg cgcttcataa aagtattcag agcttctttt ttgtccattc
       61 cgtcttttgt cattttgtgt acttctagtg ctccgtacat attcgaaatt agttcgccaa
      121 taacgtctaa ttcttcatct ttcaaggaag aaacttcggt taaagcttcc aaaacttcga
      181 tagtttcaac aaggtaatct tggtcatttt cgtcaataaa ttgagttaaa tgtttgatta
      241 taggtagttt cataattact taatttcgtt taccaattct gtcaaaactt ccgctttatt
      301 ggtttgggtt tcgtttacta atttcccatt tacgaaagta gcaaaggtag gcaagttgct
      361 cacgttcgct aattttcttg attcgggaga attttcagca tccactaaaa caaaagtcaa
      421 atcgtcattc tctgaagcca attttttgaa ctttggtttc ataattcgac aatttccaca
      481 ccaagaggcc gaaaattgaa cgattacttt ttcctcttta gcaactaaat cttgtaatgt
      541 atcttcgttt aattcgatta acatagtttt ttgaaatttc aatttcaatg tcaatgtcaa
      601 aaatcaattt ttaaattggt gcaattaaaa tgtagctatt tatttaaaat aagagtttta
      661 taaaatcaaa ttctattatt cacattgaaa ttgcaattga tttttgacat tgtaattgaa
      721 attattgtta gtgaatgtta gtgagatcct aaataagcag ctgtactatt tctgtcagct
      781 gtcattgcat cttttccttc ttcccagttt gcaggacaaa cttcgccttt agtttgaacg
      841 tgagtgtagg catctactaa acgtaaatat tcgttcacat tacgacctaa tggcatatcg
      901 tttacacttt cgtggaaaat tttaccttct tcgtcaatta aataggtagc tctgtaagga
      961 acgtttgagc ctactaatag ttcaccgtca aaagcttcgt catattccat ttcgatgtct
     1021 aaaataccta attcggcagc caaatttcta gttgtatctg caaggattgg ataagttact
     1081 ccttcgattc caccgctatt ttttggagtg tttaaccaag caaagtgtac ttcgttagta
     1141 tcgcaagaag ctccaattac gatagtgttt ctttttttga attcgcctaa agcagcttga
     1201 aaagcgtgta attctgttgg acaaacgaaa gtaaaatctt ttggatacca aaacaaaagt
     1261 actttttgat tgttttctgt agcttcttca agtacattga ttcttaaatt gtcgcccatt
     1321 tctgaaatag cgtcaactgt aatgtttggg aatttttttc ctactaatga cattgtgtta
     1381 tgttttaaag ttattattat tttttactga gacaaatgta attcaaaagc taattttgaa
     1441 atggaaattg taattatata tttttattat tgtataataa aatcttattg tatttataaa
     1501 tacaatagta attagctatt taaaagccgt ttatagttac aggtaaactt cctctgcatc
     1561 ttgaatttct tagcaattgg cgtgcggcac tttcttgaaa ttcacgaaaa tcttgataca
     1621 cttgtacgat tccaatagtt gattttaaat taggaattac atctagtaca taaggatttc
     1681 caaatacgta caaaacgcat ttctttttag aaaatagatc ttctaaaaat tctaatacag
     1741 agtcttctat accaaaatta ttcaaaggtt tggctttcgg aacaaaaaga gttacaagaa
     1801 ttgtatcaaa acattttaat tcttctttaa tgtttgctat ggctgtttta tctcccgttt
     1861 ctagtgcgaa tttttctgaa ggaagaacta gttttagtga tttataaaat atgttatcaa
     1921 tatttttata gagacataat ttagcaagtt tgtcattatt tttagcttca aacaagtgca
     1981 aaccattttg cttgtccttg attaaagttg tgcttttatc agcaattaga cgatttaata
     2041 tggaagtggc ttcaaaatcg aattctcctt ctggagcaaa atttccatct aatattcccg
     2101 ctttttgttt gcatttccac aagcgattga aactttcttc gatacgtttt ggagttgcat
     2161 ttttgaggat ttcctgaatt ccttcggcta cattttcggc aaaacacagt acatcatttc
     2221 ctgcattaaa agcttcccat tcgagttgtc cttttatatc atacagtttt gaaacgctat
     2281 gcatattcaa agcatccgaa atgactaaac catcatattt caattgttta cgcaaaagat
     2341 cttcaataat tgatttagat aaagttgccg aggtgttttt tccttcattc aatgccggaa
     2401 ctgccaaatg tccaatcata atcgaatcga cattgttttc gattccttta atgaagggaa
     2461 ctaattcgtt ttccattaat tgctccaaag tttcttctaa aataggcaat cccaagtgcg
     2521 aatcgacact tgtattaccg tgacctggaa agtgtttcag acaacctaaa acgccaactt
     2581 cattcagtcc acgtaaatat tcagtagaaa aattagcaac tctatttttg ttttgaccaa
     2641 acgaacgata gccaattacg ggattttttg ggttgttatt aatgtctgcc aaaggcgcca
     2701 aattgtaatg aattccagct gctttcaaat ccaatcctgt tcgtcttcct acttcgtaaa
     2761 ccaaatattc ataacgctct ggcaaagcac caagagaaat tgcgtaagga tattggggtg
     2821 tgttttcaac tcgcattgcc aatccccatt cggcatcaat actcatcaaa agtggagtgg
     2881 aagcgcattt ttgaaaacgt acaacgagtt cttttaaacg ttcaaaacta gagtcgttaa
     2941 cttcaacttt ttctttggat tcataattgg tagcagcact ggcacgacta tgaaaaaaag
     3001 taagtccgcc aatgttgtgt tctgtaatta aacgttcaat ttcttgaaaa ttttcttcag
     3061 tatcattaat aaaaactgca ggaaaaaaga attgaccaat tttttgttct aatgtcattg
     3121 atttagaaat ttaaactaag atgaatctca gattaaactg atttttgaat ctgttttttt
     3181 tctgagtttt tgcaattaat attgctgcat ctgagtgcta ttttttgtag gtttaaattt
     3241 agcactattt ttttaaattt caattattta attggcatta aattgttagg ttacaaagtg
     3301 aatttaattc ttttgtttta ttttttttga gcattaatta gtttgttttt tttccaaact
     3361 cttttgggaa attcaaaaat tgtgataaaa ttaaacctgg aattgtggcc aaaaacaccc
     3421 aaataaagaa attaccatag cccaaatatt cctgaatata accactcaac attccgggaa
     3481 gcatcatccc aagagccata aatcctgttg caatagagta gtgcgaggtt tttgattcgc
     3541 catcagctaa gtaaattaaa tacatcataa aggacgcaaa accaaatcca taaccaaatt
     3601 gttctgcaat tacaactgca taaatataat agatagaagt tggatgaaaa tgagccagca
     3661 aaatgaaacc aattattggc aagtgcatag ttaatatcat aggcaacatc cattttccga
     3721 gtccattttt agaaatggca attccgccca gaattccacc aattgtaagg gctaaaacac
     3781 caaaagttcc ataaatgata cctacatctt cagttaacaa tcccattcct ccaactgctt
     3841 tatcgtcaat caaaaacgga gttagcattt ttaacaattg agattcgcct aatcgaaata
     3901 atagtatgaa tgataaggct attccaattt gttttttttg gaaaaagctc gaaaaaacag
     3961 ttccaaaact cactttagga gtgggttgag attcggtttc tagcgaatta atttcggttt
     4021 ttggagttaa aaaataattg tatatcgtta aagctgtcat tattagccct acaattatca
     4081 tcgtataaga ccaagctttg gttttgtcgg catataattg ttcaaaataa ccagcaataa
     4141 ttacaatcaa tccatttcct gttagcattg ccaatctata aaaagtactt ctaattccta
     4201 agaaaaacga ttgttgatct ttagttaaag ctagcatata aaacccatca ctggctacat
     4261 cattcgaagc agaagcaaaa gccgctaccc aaaaaatggc taaactcaaa atgaaaaagt
     4321 tgctaacagg aatcgtaaat ccaactacta aaaaggcaat tgaaattaac aattgcatta
     4381 ccagaaacca atttcttttg gtcgaaaaca aatcaataaa tgggctccat aacggtttta
     4441 ttacccaagg taaatacaat aaactcgtat agattccaat atcttcgttg ctgattccta
     4501 ggcttttgta cattattacc gaaaccgaaa ttataatagc ataaggcaag cccgaagcaa
     4561 agtttaaaaa cggaatccaa taccaaggtt cattttttgt ttttgaagtt tccattttag
     4621 ttttgtttta tttcggtgtt taaattgaca atttcaggaa cactttcgtt tccataaaaa
     4681 tcgataaatg taaaagctac ttttttgata ttatttaatt ggtctttagc aatcgaatat
     4741 tttatggact cgctttttgc atcgataaat attttttcta agatttgact agggtcgttg
     4801 ctgtcaagtt tcgtattgtt ttcggtgata taaaccacta tatatcgaac ttggtctgat
     4861 tgtgtttttt tgaatgcaaa ttgatataaa tttccttctt tagaaaatga attgattaaa
     4921 ggtcgttcag ttcctgtttt tttcgaatta ggtacaggca aaggaattgc aggatattta
     4981 tattgatttg cttttaacaa gtctgcaacg tctttattga tattgacaaa agctttggca
     5041 ctataaaagg aatttccttg tatattagga aaggttcggg ttaaatcgat ttgatttgga
     5101 atttcgcctg gaacaaacca ctttttgtct gaatcatttt taattttata acttccattt
     5161 ccaatataaa cattagcatt tatagaatta ttggcccac
//
LOCUS       DAOSNN010000196         5071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_16640_length_5071_cov_5.342105, whole genome shotgun sequence.
ACCESSION   DAOSNN010000196 DAOSNN010000000
VERSION     DAOSNN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16640_length_5071_cov_5.342105"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLQ70_09685"
     CDS             complement(<1..312)
                     /locus_tag="PLQ70_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQE34924.1"
                     /translation="MKNRLANQIICVILTVAFLGFFACSSDSGSTPVVTPVTKTLTVN
                     VSSVEFLTSGNSSGISITTNVDAWTMSSSDTSWIQISKTSGTSGTTSLNITATTNTGT
                     TT"
     gene            complement(338..1909)
                     /locus_tag="PLQ70_09690"
     CDS             complement(338..1909)
                     /locus_tag="PLQ70_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQE34925.1"
                     /translation="MKNNIFASVLLICLTNVSLFGQISTDRIIKVNSNQSSGKFNTMF
                     KECVGAGRANEGLRADWQQQLAIVKKECDFKYIRMHGLLTDDMAVYREDSKGNPEYNY
                     QYVDVLFDFLISIKMKPFVELGFMPNALASGKETIFWWKGNVTPPKDYKKWEDLIRNL
                     TQHFTERYGADEVKTWYFEVWNEPNLTPGFWTGTQAEYFKLYDYAVRGIKSVNPDYKV
                     GGPATAGAGWVPETIAFCDKNNVPIDFISTHSYGVGQGFLDEFGQSGTVLSKETSSVS
                     GDVINSRKQIASSVKPNLELHYTEWSSSYTPADPIHDSYHSAAYILQKLKQVGNAANS
                     MSYWVFTDIFEEPGPRFTPFHGGFGLLNTQGIKKPAYFSYSFLNKLGETELQNTDTSS
                     WATKNKKGDVQLLFWDFTYTLPEATNNQQYYIKDLPSKYKGKVKIEVAGLQEGNYNLE
                     IYKVGYKSNDAFTDYLGMNKPNQLTKEQVKKLKENNNGSVFKIEKLTVDAKGNFSKEI
                     EMNENDVYFLNFIKQ"
     gene            2249..2914
                     /locus_tag="PLQ70_09695"
     CDS             2249..2914
                     /locus_tag="PLQ70_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQE34926.1"
                     /translation="MNEISIDCVIFGFKNGILNVLLVKHAIGDSKGEWGLPGGLILKK
                     EGIDAAAQRTLKELTGLDNIFLEQLKAFGEPNRIPLGRVITIGYYALINFDDYDVKAG
                     ITVSEAKWYKIDSIPKLIYDHNEILDFGLKKLQTRILSTPIGFNLLPEKFTLLQLMHL
                     YEEILGKELDKSNFRRKILKMKLLVALDEKQQDVSHRAANLYKFDDAIYKKLSQKGFN
                     FEI"
     gene            complement(2920..3936)
                     /gene="lpxK"
                     /locus_tag="PLQ70_09700"
     CDS             complement(2920..3936)
                     /gene="lpxK"
                     /locus_tag="PLQ70_09700"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017494871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQE34927.1"
                     /translation="MNLVRKIFFPFAILYGIITSIRNFLFDKGILKSYSFDIPVIAVG
                     NLSVGGTGKTPQIEYLIRLLSSKYKVATLSRGYKRKSEGFVLANATSNAEILGDEPFQ
                     FYQKFKNIQVAVDADRKNGIEQLLSQLEKPDIILLDDAFQHRKVKAGFYILLTSYGDL
                     YSDDFMLPTGNLRESRSGAKRANLVIVTKCPSHLSLDEQNIIKDKLNLVSNQELYFSF
                     IDYDDSIYSEEKSMKVSEIKSVDKLLVAGIAKPEPFFNHLQSQNDEKLVFPDHHHFSE
                     NDLLEIKNKTQNKIILTTEKDFMRLKGKLPKEQLFYLPIRSSFLSNGESFDKTITDFV
                     QSKN"
     gene            4046..>5071
                     /locus_tag="PLQ70_09705"
     CDS             4046..>5071
                     /locus_tag="PLQ70_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQE34928.1"
                     /translation="MKIKEIISVLEEMAPLAYAEDFDNVGLLVGNQNWEATGALVCHD
                     ALESVIEEAIAKNCNLVVCFHPILFSGLKKITGKNYVERSIIKAIKNDIAIYAVHTAL
                     DNHQEGVNKIFCDALGLTNTKILIPKSNFIRKLVTYTIPENAEKLRNALFDAGAGNIG
                     NYENCSFNSKGIGTYMGNEHSNPEIGERFEFVENEEIKIEVTFEKHLENKILKALFKN
                     HVYEEVAYEIYDLQNKNQNMGLGMIGELPAPMDEIAFLNLVKDKMQADGIRHSSFLGK
                     SIKKVAVLGGSGSFAIKNAIQAGADAFLTADLKYHQFYEAENQLILADIGHFESERYT
                     KNYIVDFL"
BASE COUNT         1675 a          860 c          829 g         1707 t
ORIGIN      
        1 agtggtagtt ccagtatttg tagttgctgt aatattgagc gaagttgttc ctgatgttcc
       61 tgatgtttta cttatttgaa tccaactagt atcagaacta ctcattgtcc aagcatcgac
      121 attggttgtg atgctaatac ccgaactatt tcctgaagtt agaaattcaa cactacttac
      181 atttactgtt agcgtttttg ttactggagt tacaactgga gttgagccag aatctgaact
      241 acaagcaaaa aatcccaaaa aagccacagt caaaatgaca catattattt gattagcaag
      301 tctatttttc atttggtttt gtatttaaat ttgaatctta ttgtttaatg aaattgagga
      361 aatacacatc attttcgttc atttcaattt cttttgaaaa attccctttt gcatctacag
      421 taagtttttc tattttaaaa accgatccat tattattttc ttttagcttt tttacttgtt
      481 cttttgtcaa ctgattcggt ttattcatcc ctaaataatc ggtaaaagcg tcgtttgatt
      541 tataacctac tttgtaaatt tctaaattat aattaccttc ttgcaaacct gcaacttcaa
      601 tttttacttt tcctttatac tttgaaggta aatcttttat ataatattgt tgattgttgg
      661 tagcctctgg caaagtgtag gtaaaatccc aaaacagcag ttgcacatct ccttttttat
      721 tttttgttgc ccaagaagaa gtgtcagtat tttgaagttc ggtttcgcct aatttattca
      781 aaaatgaata cgaaaaataa gcgggttttt taattccttg agtattcaac aagccaaaac
      841 caccgtgaaa aggggtaaat cgtggtcctg gttcttcgaa aatatcagta aaaacccaat
      901 acgacatcga gttggcggca ttccccactt gtttcagttt ttgaagaata taagcagccg
      961 aatggtaact gtcgtgaatg ggatcggctg gtgtgtaaga ggaactccat tcggtataat
     1021 gcaattcgag attgggtttg actgaagagg caatttgttt acgagaatta ataacatcgc
     1081 cacttacgct cgaagtttct ttactcaaaa ccgttccgct ttgtccaaat tcatctaaaa
     1141 aaccttgtcc aacaccgtag ctatgggttg agataaaatc tattggcaca ttatttttat
     1201 cgcaaaatgc tatcgtttca ggaacccaac ccgctccagc tgttgcaggg ccacctactt
     1261 tgtaatctgg atttacactt tttattcctc taacagcgta atcatagagt ttgaaatatt
     1321 ctgcttgagt gcccgtccaa aatcctggag ttaaattggg ttcattccaa acttcgaaat
     1381 accaagtttt tacttcatca gctccataac gttctgtaaa atgttgggtt aaatttcgaa
     1441 ttaaatcttc ccatttttta taatcttttg gaggagttac gttgcctttc caccaaaaaa
     1501 tggtttcttt tccactggcc aaagcattag gcataaaccc caattcaaca aaaggtttca
     1561 tcttgatact tataagaaaa tcgaataaaa catcgacata ttggtaattg tattctggat
     1621 ttcctttgct atcttcacga taaaccgcca tatcatcggt caataaaccg tgcattctaa
     1681 tgtatttgaa atcgcattct ttcttgacta tagccaattg ctgttgccaa tcggcacgca
     1741 atccttcatt ggctcttcct gctccaacac attctttgaa catggtgttg aattttccag
     1801 acgattgatt cgaattcacc ttaatgattc tatctgttga aatttgtcca aacaagctaa
     1861 cattggtcag acagattagc aaaactgatg cgaaaatgtt atttttcatg atactttaat
     1921 atattttttt taatcgaata cattatttca tacttatggt aaatttgaca acaagtttac
     1981 tgttttattt ttacatttgc aatatggata gccatatatt tatgtatgtc ataatatatt
     2041 atttatgttt ttactaaatc atcaataatt acaacatttt atatttttaa atagcacatt
     2101 tatttattaa tattgaagtc taaagagtcc tttttcgaaa gaaaattaat ttaccatgag
     2161 aaatcattaa atttaatttc aaaaaaaata gaatgaagtc caataaaatc caaacaaaga
     2221 atcaataact tctaatactc taacaactat gaatgaaatt tctattgact gtgtcatttt
     2281 tggttttaag aacggtatcc ttaatgtcct tttggtaaaa catgccattg gcgacagcaa
     2341 aggcgaatgg ggacttcctg gtggtttgat tttgaaaaaa gaaggaattg atgctgctgc
     2401 tcaaagaaca ctgaaagaac ttacgggttt agataatatt tttttagaac aactaaaagc
     2461 ttttggagaa cccaatcgaa ttcctttggg aagagtcatc acaattggat attatgcttt
     2521 aattaatttt gacgattatg atgtaaaagc aggaattacc gtatctgaag ccaaatggta
     2581 taaaatagat tctattccta aattaattta tgaccacaat gaaattttag attttggtct
     2641 gaaaaagttg caaactcgca ttctatcaac cccaatagga tttaatttat tgcctgaaaa
     2701 atttacttta ttgcaattaa tgcatcttta tgaagaaatt cttggcaaag aattagacaa
     2761 atcgaacttt agaagaaaaa ttttgaaaat gaaattactt gtagccctag acgaaaaaca
     2821 acaagatgta tctcatcgcg cagccaattt atacaaattt gacgatgcta tttacaaaaa
     2881 actcagccaa aaagggttta attttgagat ttaaacccgc tagtttttag attgaacaaa
     2941 atcagtaata gttttatcaa agctttcacc attagaaaga aaagaacttc gaatgggtaa
     3001 atagaaaagt tgctctttgg gcaatttccc tttcaaacgc ataaaatctt tttcggtggt
     3061 aaggattatc ttgttttgtg ttttgttctt aatttctaat aaatcatttt ccgaaaaatg
     3121 atgatggtct ggaaaaacca atttttcatc attttggctt tgcaaatgat tgaaaaacgg
     3181 ctccggtttg gcaattcctg ccacaagcaa tttatctaca gacttaattt cactcacttt
     3241 cattgacttt tcttccgaat aaatagagtc atcataatca atgaaagaaa aatacaattc
     3301 ttgatttgaa accaaattca atttgtcctt aattatattt tgttcgtcaa gcgaaagatg
     3361 ggatggacat tttgtaacaa taaccaaatt ggctcttttt gctccgctcc tactttctcg
     3421 cagatttcct gttggcaaca taaaatcatc cgaatacaaa tcaccatacg aagtcagtaa
     3481 aatatagaaa cctgctttta cttttcggtg ttgaaaagca tcgtccagca aaataatatc
     3541 gggcttttcg agttgagaaa gcaattgctc aataccattt tttctatcgg catcaacggc
     3601 aacctgaatg tttttgaatt tttgataaaa ctgaaatggt tcgtctccca aaatttcagc
     3661 attcgaagta gcattggcca aaacaaaacc ttcggatttt cttttgtaac cacgacttaa
     3721 agtagccact ttatattttg aagaaagcaa ccgaattaaa tattcgattt ggggtgtttt
     3781 tccggttccg cccacgctga gatttccaac ggcaataacg ggaatatcaa atgaataaga
     3841 tttcaaaatt cctttatcaa aaagaaaatt ccgaatactg gtaatgattc catacaaaat
     3901 ggcgaaagga aagaatattt ttcgaactaa attcatacta cgaaagtata ataatttatc
     3961 tttgaattaa aattttgttt cttttgttta atgtttgaac ttttcaaatt aaaacttcaa
     4021 gccttaaact tttaacctaa aaacaatgaa aatcaaagaa ataatttccg tactcgaaga
     4081 aatggcacca ttagcttatg ccgaagactt tgacaatgta ggtttattgg ttggaaatca
     4141 aaattgggaa gcaacgggag ctttggtttg tcacgatgcg ctcgaaagtg taattgagga
     4201 agctattgca aaaaattgca atttggtggt ttgttttcat cctattttgt tttccggttt
     4261 gaagaaaatc acgggcaaaa actatgtaga acgctctata atcaaagcca taaaaaatga
     4321 cattgctatt tatgccgttc ataccgcctt ggacaatcat caagaaggcg tcaacaaaat
     4381 attctgcgat gctttggggt taaccaatac taaaattcta attccgaagt cgaatttcat
     4441 tcgaaaatta gtcacatata ccattccaga aaatgctgaa aaactccgaa atgccttgtt
     4501 tgatgctgga gcaggaaata ttggcaacta cgaaaattgt agtttcaact ccaaaggaat
     4561 tggaacttat atgggcaatg aacacagcaa tcccgaaatt ggagaacgct ttgaatttgt
     4621 agaaaatgaa gaaataaaaa tagaagtcac tttcgaaaaa catttggaaa acaaaatcct
     4681 gaaagcactt ttcaaaaatc acgtctatga agaagtggct tatgaaattt atgatttgca
     4741 gaacaaaaat caaaatatgg gattaggaat gattggcgaa ttaccagctc caatggatga
     4801 aatagctttt cttaatctgg taaaagacaa aatgcaagct gatgggattc gacacagctc
     4861 ttttttaggc aagtcgataa aaaaagtggc cgttttgggc ggttcaggaa gttttgctat
     4921 aaaaaatgct attcaggctg gagccgatgc ttttttgacc gccgatttga agtatcatca
     4981 gttttatgaa gccgaaaatc agttaatttt agccgatatt ggtcattttg aaagcgaacg
     5041 ctatacaaaa aattatattg ttgattttct t
//
LOCUS       DAOSNN010000197         5037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_16778_length_5037_cov_4.287435, whole genome shotgun sequence.
ACCESSION   DAOSNN010000197 DAOSNN010000000
VERSION     DAOSNN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16778_length_5037_cov_4.287435"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLQ70_09710"
     CDS             <1..693
                     /locus_tag="PLQ70_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE34929.1"
                     /translation="AIKRQITVLENKISILIGQAPAPIERGKTNSEFQIASTIPVSIP
                     SILLKNRPDVNQAERLYMAANERIGVAQAMRFPSFNIAALAGFANSSVSNLFDGSSYL
                     QNASTGVTGPIFNFGKNKRRVEIYRQIAEESRLSYQKTCITAVAEVEQALQNVRTYKE
                     EWTARNRQVIAARKNLELSNARYYNGYVSYLEVIDIERSLFDAELKLSELAQNQLSST
                     IELYRALGGGWN"
     gene            complement(817..2469)
                     /locus_tag="PLQ70_09715"
     CDS             complement(817..2469)
                     /locus_tag="PLQ70_09715"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HQE34930.1"
                     /translation="MTQSLIVLFLAISVGFFVGKIKFGNISLGVSAVMFVGLFLGHLG
                     FTMNTELLQFVREFGLILFVYGIGIQVGPTFFSSFKKEGLVFNALGVGTVFLGGIITY
                     LIHLLANIKVENAVGLMSGSVTNTPGLGAAKSTLIEIQKQLNLPADHFSDPAIAYAIT
                     YPVGVFGIILIIILAKNFLKIDLNKEVKLLQEKNKDSENKIVRQKCRVTNPAVFGKTI
                     KQILKEFKIENVIISRQKHSGTTIVYSPSLDAIVNHKDVLMVVAKQKDIDNFIENVGK
                     VSTDLFIESEDDITTKIISVTQKTATHKTLAQLDLYNQYDVRVTRVIRSGLELLAQPS
                     LELFYGDTLMVVGNRQAIEEAEKLIGNSKKILLEPDFLSLFGGLIFGVVIGSIPFFIP
                     SLPVPLKLGLAAGPLLAALFISRYGGVGVIHSYINNGAIHFMKDFGICLFFAAVGIKA
                     GHGFYDSFIENNGWMWIYYGCFITFIPLTILVLISRFVFKLNFYQMVGIMSGSYTDPA
                     ALAFSTKYLDSDIPNQSYATVYPLVTISRILVAQLLILLFAS"
     gene            complement(2560..3933)
                     /locus_tag="PLQ70_09720"
     CDS             complement(2560..3933)
                     /locus_tag="PLQ70_09720"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HQE34931.1"
                     /translation="MSNDPQKITEDRKTQSVLHLLTEIGEMMITSGAHTARIIRNLER
                     IAIGLGYHCELVLTYTGIVTSVYKENKHKAHTLARTIKGKGLNFETISEISILSWDVF
                     ENKISIEEIKETLKQIKAKKVYSDLQLYALAPFASVALCMLFDGDWLQSVIVYFSTLF
                     GYYARRSLMLKHHNHMFAFFIGATISTLFIHFSGVFFNVQIKEAMVVSVLYLIPGAVL
                     INSFIDYLEGYFESGTARFIYSFMLILMIAFGFFTSTMIYNIPAMNEFQFFDLSSLQT
                     QIKEAFEPQGFGIETSKLIFGGVTSLGFALMFNTPKRALWTVFILGAFGYLIKFILFK
                     ELDMNLTLSIFIASSFVGISGMYFAHRAHTPPIIFMIPAVINMIPGLISYEFMMGMID
                     WISGDKNQKPAVEEVIQTFSYGITTVFILFALAFGVAFPVVVFKSYTVKGKDLNVMIK
                     KLFVRNN"
     gene            4163..>5037
                     /locus_tag="PLQ70_09725"
     CDS             4163..>5037
                     /locus_tag="PLQ70_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014937789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HQE34932.1"
                     /translation="MKNIKIIQELHDLGITGYHEVAYNPSYEELYQAEVSHKRKGFEK
                     GALTDTGCVAVKTGIFTGRSPKDRYIVKDEVTKDTICWDKESGISTPNYPTTKEVWDD
                     LKALTLEQLSTSPKLYVVDAFCGTNVDTRLKVRFVMEVAWQAHFVTNMFIRPSIYELE
                     NFGEPDFIVMNASKATNPNWKEQGLNSENFVLFNLTEKIQIIGGTWYGGEMKKGMFAM
                     MNYYLPLKGMASMHCSANVGPEGDVAVFFGLSGTGKTTLSADPKRYLIGDDEHGWDNN
                     GVFNYEGGCYAKVIDL"
BASE COUNT         1834 a          842 c          805 g         1556 t
ORIGIN      
        1 gctattaaac gccaaataac tgttcttgaa aacaaaattt caattctaat tggtcaagca
       61 ccagcaccaa tagaacgagg taaaaccaat tcagaatttc aaatagctag tactattccg
      121 gtttcaattc catctatttt gttaaaaaac agaccggatg tgaatcaagc cgaacgattg
      181 tatatggctg ccaacgagcg aataggcgtg gcgcaagcaa tgcgttttcc atcgtttaac
      241 attgcagcat tagctggttt tgctaatagt tctgtcagca atttatttga tggttcttcc
      301 tatttgcaaa atgctagtac aggtgtaact ggaccgattt ttaattttgg aaaaaacaaa
      361 agaagagtag aaatctaccg tcaaattgct gaagaatcaa gactttctta ccaaaaaact
      421 tgtattacgg cggttgccga agtggagcaa gctttgcaaa acgtaagaac ttacaaagaa
      481 gaatggacag ctcgaaaccg tcaagtaatc gctgccagaa agaacttaga attatcaaat
      541 gccagatatt ataacggtta tgtttcttat cttgaagtta ttgatattga acgttctttg
      601 tttgatgctg aattaaaact ttcggaatta gcacaaaatc aattaagttc tactatcgaa
      661 ttgtatagag ctttaggtgg tggttggaat tagttcattt tcaaaaatat attttaaaaa
      721 cttgtcttct attggcaagt tttttttgtg atccaaataa aaaaagggct actcaccaat
      781 agtgaaatag ccctttgggt tgaaatatat agagtatcaa ctcgcaaaaa gcaagatgag
      841 taattgggcc actaaaattc tactgatagt cactaatggg taaactgttg cataggattg
      901 attaggtata tcagaatcca aatattttgt actaaaagct aaggcagctg gatcggtata
      961 agaaccactc ataattccga ccatttgata gaaatttaat ttaaaaacaa atcggctaat
     1021 caatactaat atcgtcaatg gaatgaaagt gataaaacag ccataataaa tccacatcca
     1081 gccattattt tcaataaaac tatcataaaa accatgacct gctttaattc ctactgcagc
     1141 aaaaaataaa cagataccaa aatctttcat aaagtgaata gcaccattat tgatataaga
     1201 gtgaatgacc ccaacaccgc catatctact aataaataaa gcagctaaaa gtggtcctgc
     1261 agccaatcct aattttagag gaactggcaa agaaggaata aaaaatggaa tagaaccaat
     1321 gaccacacca aaaatcaaac ctccaaaaag agagagaaaa tcaggttcca aaagtatttt
     1381 tttagaattt ccaatcaatt tttctgcttc ttcaatggct tgtctatttc caacaaccat
     1441 taaagtatct ccataaaaca actctagtga aggttgtgct agaagttcta atcctgaacg
     1501 aataacacga gtgactctaa catcatattg attatataaa tccaattgtg ccaatgtttt
     1561 atgagtagct gttttttggg ttacacttat gatttttgtt gtaatatcat cttcagattc
     1621 aataaataaa tcggttgaaa ctttaccaac attttcaata aaattatcaa tatctttttg
     1681 ttttgctaca accatcaaaa cgtctttgtg attgacaata gcatctaatg atggagaata
     1741 tacaatggta gttccgctat gtttctgtct cgaaataatt acattttcaa ttttgaattc
     1801 tttcaaaatt tgtttaatag tttttccaaa aacagcagga tttgtcactc tacatttttg
     1861 acgaacaatt ttattttcag aatctttatt tttctcttgg agtaacttaa cttctttatt
     1921 taaatcaatt ttcaaaaagt ttttggctaa aatgattata agaataattc caaaaacccc
     1981 aacaggatat gtaatcgcat aagcaatggc tggatccgaa aagtgatcgg ctgggaggtt
     2041 taactgtttc tgaatttcaa taagagtaga ttttgcagcc cccaaaccag gtgtgtttgt
     2101 taccgaacct gacattaatc caacagcatt ttctacttta atgtttgcca acaaatgaat
     2161 aagataggtg ataattcccc ctaaaaatac agttccaaca cctaaagcat taaacaccaa
     2221 accttctttt ttaaaggatg aaaagaaagt aggtcctact tgaataccaa ttccataaac
     2281 aaacaatatc aaaccaaatt cacgaacaaa ttgaagtaat tcagtgttca tcgtaaagcc
     2341 aagatgacct aagaaaagac caacaaacat tacggcagaa acccctaagg aaatatttcc
     2401 aaacttaatt tttccaacaa aaaagccgac acttatagcc aaaaataata caattaatga
     2461 ctgtgtcatc tccggctcat cagtcggagt gaataaaaat ttaaaccatt caaacataat
     2521 atgaaaattt aataattaga catttagaaa aataatattt tagttgttcc ttacaaatag
     2581 ctttttaatc attacattca agtcttttcc tttaacggta taggatttga aaacaacaac
     2641 tggaaatgca actccaaagg ctagagcgaa gagtataaat acggtggtta ttccataaga
     2701 aaaggtttgt atcacttctt caacggctgg tttttgattt ttatcaccac ttatccaatc
     2761 tatcatcccc atcataaatt cataacttat taaacctgga atcatattga ttactgctgg
     2821 aatcataaaa ataattggtg gagtatgagc tctgtgagca aaatacattc cagaaatacc
     2881 aacaaatgaa gaggctataa atatagataa ggtcaaattc atatctaatt ctttaaacaa
     2941 gatgaattta attaaatatc caaaagcacc aaggataaat acagtccata aagctctttt
     3001 aggggtgttg aacatcaatg caaatcctaa agaagtgact cccccaaata ttaatttaga
     3061 agtttcaatt ccaaaacctt gaggttcaaa agcttctttt atttgtgttt gcaaggagga
     3121 taaatcaaaa aactgaaatt cgttcattgc aggtatgtta taaatcatag tgctggtaaa
     3181 aaaaccaaaa gctatcatca aaataagcat aaaactatag ataaagcgtg ccgttccgga
     3241 ctcgaaataa ccttctagat aatcaataaa agaattgatt aataccgccc caggaattag
     3301 atacaatacc gatactacca ttgcctcctt gatttgaaca ttaaaaaaga ctcccgaaaa
     3361 atggataaat agggtagaaa tagtggcgcc aataaaaaaa gcaaacatat gattatgatg
     3421 ctttaacatt aatgatcttc tagcataata tccaaataaa gtcgaaaaat agactattac
     3481 ggattgcaac caatcgccat caaacagcat acaaagtgct acacttgcaa atggcgccaa
     3541 agcatataat tgtaaatccg aatatacttt tttggctttt atttgtttta aagtttcttt
     3601 aatttcttca atcgaaatct tattttcgaa gacatcccaa gataaaatac tgatttcgga
     3661 tatggtttca aaatttaaac ctttgccttt tatggttctt gctagtgtat gggctttgtg
     3721 tttattctct ttataaaccg atgtaacaat tccggtataa gtcaacacta attcacaatg
     3781 atatcctaaa ccaatagcaa ttctttctaa gttccgaatg attcttgctg tatgtgcgcc
     3841 actagtaatc atcatttcgc caatttcagt caataaatgg agtactgatt gtgtttttct
     3901 atcttcggtt attttttgag gatcgtttga catgtttgtg tatatttgga aataaatttc
     3961 ctattatttc tttgtcaaaa ttactaattt attatttagg aaatatgata tttatcatat
     4021 aaaaaacctc gcgaggcctt gtaaacaaag aaaacgtttt cgtagatttt ttgaaaatat
     4081 gataaatatc aggtttgtta gctgagaaat tcgtaatttt ggaaaaggaa attaaacaaa
     4141 taaacaaata acaaaaacaa ttatgaaaaa cattaaaatt attcaagagt tacatgattt
     4201 aggaattact gggtatcatg aagtagccta taatccttct tatgaagaat tgtatcaagc
     4261 tgaagtatct cacaaaagaa aaggattcga aaaaggagct ttgactgata caggatgtgt
     4321 tgctgtaaaa acaggtattt ttacaggacg ttctccaaaa gacagatata ttgtaaaaga
     4381 tgaagtaact aaagatacca tttgctggga taaagaatca ggaataagca ctcctaatta
     4441 tccaacaacc aaagaagttt gggacgattt gaaagcgctt actttagagc aactttccac
     4501 ttcacctaaa ttatatgttg tagatgcatt ttgtggtaca aacgtagata caagacttaa
     4561 agttcgtttc gtaatggaag tagcttggca agcacacttt gtgactaata tgttcatccg
     4621 accttcaatt tatgaattgg aaaactttgg tgaacctgat ttcattgtaa tgaatgcttc
     4681 taaagcgact aatccaaact ggaaagaaca aggcttaaat tctgaaaact tcgtattatt
     4741 taatcttact gaaaaaattc aaataatcgg tggtacttgg tacggtggag aaatgaaaaa
     4801 aggaatgttc gctatgatga actactactt gcctctaaaa ggaatggctt ctatgcactg
     4861 ttcagcaaac gttggaccag aaggcgatgt tgccgtattc ttcggtcttt caggaacagg
     4921 aaaaactact ttatctgccg acccaaaaag atatttaatt ggtgatgacg aacacggatg
     4981 ggataataat ggtgttttca actacgaagg tggatgttat gcaaaagtaa ttgacct
//
LOCUS       DAOSNN010000198         5034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_16789_length_5034_cov_4.944366, whole genome shotgun sequence.
ACCESSION   DAOSNN010000198 DAOSNN010000000
VERSION     DAOSNN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16789_length_5034_cov_4.944366"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1099
                     /gene="purU"
                     /locus_tag="PLQ70_09730"
     CDS             245..1099
                     /gene="purU"
                     /locus_tag="PLQ70_09730"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HQE34933.1"
                     /translation="MQKITILIHCEDQKAIIASVTNYIASKDGNIIYLDQHVDANENV
                     FFMRLECEFSAKNWDLESLKTDFKTQLATPFKMFWEMYTQEEKPKMALFVSKYDHCLY
                     DILGRYSAGELPLEIPVIISNHEDLKPIAERFSIPFYHVPFTKEIKEEGELAQLDLLQ
                     HYKIDFIVLARYMQIITPNLIAKYRNKIINIHHSFLPAFPGAKPYHSAFKRGVKIIGA
                     TSHYVTEGLDEGPIIEQDIARVSHSLSIEDFIMKGRDLERLVLARAIKLHAEHKTMVY
                     DNKTVVFS"
     gene            complement(1535..2320)
                     /locus_tag="PLQ70_09735"
     CDS             complement(1535..2320)
                     /locus_tag="PLQ70_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQE34934.1"
                     /translation="MINYITYTNENSDQWVTFVHGAGGSSSIWFKQIRDFKSQYNVLL
                     LDLRGHGDSKSVIKKAFQKKYTFEAIANDVLEVIDHLKIEKSHFVGISLGTILIRQLA
                     EMHPERVQSMILGGAILKMNFRSQILMKLGNTFKYVLPYLVLYQFFAFVIMPKKSHKQ
                     SRLLFINEAKKLYQKEFIKWFKLTAEINPLLKWFRQVELNIPTLYVMGGEDYMFLPSV
                     RLVVEKHYKSSKLFVIERCGHVVNVEQPMVFNNEVLSFIGSIE"
     gene            complement(2488..3300)
                     /gene="pyrF"
                     /locus_tag="PLQ70_09740"
     CDS             complement(2488..3300)
                     /gene="pyrF"
                     /locus_tag="PLQ70_09740"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQE34935.1"
                     /translation="MTTQQLIDQIKNKKSFLCVGLDVDLNKIPQHLLALEDPIFEFNK
                     AIIDATHDLAVSYKPNTAFYEAYGIKGWQSLEKTINYINENHPDIFTIADAKRGDIGN
                     TSSMYAKAFFEDLNFDSVTVAPYMGKDSVEPFLAFENKHTIMLALTSNEGAFDFQTLN
                     VDGTELYKQVLETSKNWKNSENLMYVVGATKAEYFTEIRKIVPDSFLLVPGVGAQGGS
                     LSEVCKYGMNANVGLLINSSRAIIYASNGTDFAEKAREEALKMQHEMEEILK"
     gene            complement(3379..3738)
                     /locus_tag="PLQ70_09745"
     CDS             complement(3379..3738)
                     /locus_tag="PLQ70_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015480906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HQE34936.1"
                     /translation="MRNFRDLEVWQSSVLFVKRIYTITGSFPNEEKFGLVSQINRCAV
                     SIPSNIAEGCSRSSQKDFSRFLQISLGSAFELETQIEIAKEIGFVDVILHLEIIAELN
                     VIQKRIQSLKKYVDSQV"
     gene            complement(3812..4888)
                     /gene="prfA"
                     /locus_tag="PLQ70_09750"
     CDS             complement(3812..4888)
                     /gene="prfA"
                     /locus_tag="PLQ70_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQE34937.1"
                     /translation="MLDRLQYVKQRFDEISDLIIQPDVIADQKRYVLLNQEYKGIKAL
                     VEKREEYILVLANIDEANEIIADGSDADMTEMAKMQLEEAKERLPQLEEEIKFMLIPK
                     DPEDAKNVMVEIRAGTGGDEASIFAGDLFRMYTKYCEGMGWRTSVVDMNEGTSGGFKE
                     VIFEVTGEDVYGTLKFEAGVHRVQRVPQTETQGRVHTSAATVMVLPEAEEFDVQIDMN
                     DVRVDFFCSSGPGGQSVNTTKSAVRLTHIPTGLVAQCQDQKSQHKNKDKALIVLRSRL
                     YEMELAKKQAEDATKRTSQVSSGDRSAKIRTYNYAQGRVTDHRVGLTLYDLGNIMNGD
                     IQKIVDELQLVNNMEKLKEASEVF"
BASE COUNT         1570 a          978 c          849 g         1637 t
ORIGIN      
        1 aatcaactac aaaatacgct aacaaactat ttattatcaa aaataagagt agttttgagg
       61 aaaattagga atttatttct agccctgatg gaaatggaaa gctcggaact ttagaaagtt
      121 atttttctta gcagaaaaaa gcgacaaata ccgatagcta tcgggacttt ttctggctta
      181 aaaaaatact ttataaagtg aggacttgaa atgtacagca ggattagctt caaattaaac
      241 aattatgcaa aaaattacca ttcttataca ttgtgaagat caaaaagcaa ttattgcttc
      301 tgttaccaat tatattgctt caaaagatgg aaacatcatt tatttagacc aacacgtaga
      361 tgccaacgaa aacgtgtttt ttatgcgttt agaatgtgaa ttcagtgcca aaaactggga
      421 tttggaatcc ttgaaaaccg attttaaaac tcagttagcc acaccattca aaatgttttg
      481 ggaaatgtac actcaagaag aaaaacccaa aatggctttg tttgtttcaa aatacgatca
      541 ttgcttgtat gatattttag gacgatacag cgctggagaa ttgcccttag aaattccagt
      601 aatcataagc aatcacgagg atttaaaacc aattgccgaa cgtttttcta taccgtttta
      661 ccatgttcct tttactaaag aaattaaaga agaaggcgaa ctagcccaat tggatttgtt
      721 acaacactat aaaatagatt tcattgtttt ggcgcgatat atgcaaatta tcacacccaa
      781 cttgattgca aaatacagaa acaaaatcat caatatccat cactcctttt tacctgcttt
      841 tccaggggca aaaccctatc attcggcttt caaaagaggg gtgaaaatta ttggtgctac
      901 cagccattat gttaccgaag gtttggatga agggccaatt attgagcaag atatcgcccg
      961 agtttcacac agtctttcta ttgaagattt tattatgaaa ggacgtgatt tagaacgtct
     1021 agttttggca agagccataa aattacacgc cgaacacaaa acaatggttt atgataataa
     1081 aacggtggtg ttttcgtaaa aattccttct gtaatttggt tttacattaa ttcacaactg
     1141 caataaatat gagttctttg ttcgtttttc ttaatttaaa aaaacattaa cggaactcca
     1201 acaaagtaat aacatatcat taacagcaaa aaaaggaaaa ctatctgatc tttgcagtgt
     1261 aataaactgg ttatttatta ttttggttag gttagttaaa aatatgccga tatcttagga
     1321 tgtcggcatt tttttttgca gaatctattt ggcaaaaaaa ctacctcatc taccataaaa
     1381 agttaacgga actacaacaa actaataaca tatcattaac agcaaaaaaa ggaaaactgt
     1441 cagatctttg cagtgtaata aactggttat ttattatttt ggttaggtta gttagtaaaa
     1501 aattgtcgat gtctttggat gtcggcattt ttttttattc aatagaacca ataaatgata
     1561 agacttcatt attgaaaacc attggttgct cgacattgac tacgtgacca catcgttcaa
     1621 taacaaataa ttttgaagat ttataatgct tttcgaccac caatcgaacc gagggcaaaa
     1681 acatataatc ttcccctccc attacataaa gcgttgggat attcaattct acttggcgaa
     1741 accatttgag caatggattg atttcggcag tgagtttgaa ccattttata aattctttct
     1801 ggtataattt tttggcttcg tttataaaaa gcaatcgcga ttgtttgtga ctttttttag
     1861 gcataatcac aaaagcaaaa aattggtaca aaaccaaata aggcaaaaca tatttaaaag
     1921 tatttcctaa tttcatcaag atttgagaac ggaaattcat tttcaaaatg gctccaccca
     1981 gaatcatact ttgcacacgt tcgggatgca tttcggccaa ttgtctaatc aaaatcgttc
     2041 cgagtgaaat gcctacgaaa tgtgattttt ctatctttag atgatcgata acttccaaaa
     2101 catcattggc aatggcttcg aaagtatatt ttttttgaaa cgcttttttt ataaccgatt
     2161 tcgagtctcc gtgacctctc aagtccaaca acaacacatt atactgactc ttaaaatccc
     2221 taatttgctt gaaccaaata gacgaactac cacccgctcc gtgaacaaaa gtcacccact
     2281 gatcactgtt ttcgtttgtg tatgtgatgt agtttatcat tattttaaat ggaacacaga
     2341 ttaaacggat tcgcaaaagc gaaaacacgg attaaaacgg attttttcaa taaatttaat
     2401 tcgttgcaat gtagaaaaat ttcagccacg aatttcacct attctcacga attaatttgt
     2461 ggaaatttgt gtaatttgtg gctagtttta ttttaaaatt tcttccattt catgttgcat
     2521 tttcagggct tcttcccttg ccttttcggc aaaatcagtt ccgttggaag cataaataat
     2581 cgctcttgaa gaattaatta ataatccaac attggcgttc attccgtatt tacaaacttc
     2641 agaaagactt cctccttgtg cgccaacgcc aggaaccaat aaaaaactat cgggaacaat
     2701 tttgcgaatt tcagtaaaat attccgcttt ggtagcgcca accacataca tcaggttttc
     2761 ggaatttttc caatttttag aagtttctaa aacttgtttg tacaattctg ttccgtccac
     2821 atttagggtt tgaaaatcga aagcgccttc attggaagtt aaggctaaca ttatggtgtg
     2881 tttgttttcg aaagccaaaa acggctccac cgaatctttt cccatataag gtgcaacggt
     2941 aacactatca aagttcaaat cttcgaagaa agctttggca tacattgatg aagtattgcc
     3001 aatatcaccc cgtttggcat cagcaatcgt gaaaatatcg ggatggtttt cgttgatata
     3061 gttgatggtc ttttcaagag attgccagcc tttgatgcca tacgcttcat aaaacgccgt
     3121 attgggttta taggaaacag ccaaatcgtg tgtggcgtca atgatggctt tgttgaattc
     3181 gaaaatcgga tcttcgagtg ctaataaatg ttgtggaatt ttattcaaat cgacatccaa
     3241 tcctacgcaa aggaaagatt tcttgttttt gatttggtct atgagttgtt gtgtagtcat
     3301 tttttggtat tgggtgttgg gtgttgggtg ttgggtgttg ggtgttgggt gttgggtgct
     3361 gggtgctggg tgctgggttt agacttgaga atcaacatat ttttttaagg actgaattct
     3421 tttttgaatt acattaagct cggcaataat ttctaagtgc aaaattacat ccacaaaacc
     3481 tatttcttta gcaatttcaa tttgagtctc taattcaaag gcagaaccta aacttatctg
     3541 taaaaacctg gaaaaatcct tttgagaact tctggaacaa ccttcggcta tgttcgacgg
     3601 aattgaaacc gcacatctat taatttgaga aaccagtcca aatttttctt cgtttggaaa
     3661 agaacctgtt attgtgtaaa ttctttttac aaataaaaca gaactttgcc aaacttctaa
     3721 atccctaaag tttctcatat attttatttt ttactaaaat cagcaccaaa aacctaagac
     3781 ctaacaccca agacccagca cccagcacct gctaaaatac ctcgctcgct tctttcaatt
     3841 tctccatatt gttgaccaat tgcaattcgt ccacaatttt ctggatgtcg ccattcatta
     3901 tgtttcctaa atcgtacaaa gtcaaaccga ctctatggtc ggttacacga ccttgcgcat
     3961 aattgtaggt tctaattttg gccgaacggt cacccgaact tacttgcgaa gtacgtttgg
     4021 tcgcatcttc cgcttgtttt ttggccaatt ccatttcgta taaacgagaa cgcaaaacga
     4081 ttaaggcttt atctttattc ttgtgttgcg atttttggtc ttgacattgc gccaccaatc
     4141 cagttggaat gtgcgtcaaa cgaaccgccg atttcgtagt atttaccgat tgtccaccag
     4201 gtcctgacga acagaagaaa tccacacgaa catcgttcat atcaatttgc acatcaaatt
     4261 cttcggcttc tggaagcacc ataaccgtcg ctgccgaagt atgaacacga ccttgagttt
     4321 ccgtttgtgg cacacgttgc acacggtgaa cacccgcttc aaattttaaa gttccataaa
     4381 catcttcgcc cgtaacttca aaaatcacct ctttgaaacc gcccgaagtg ccttcgttca
     4441 tatccacaac cgaagttctc cagcccattc cttcgcaata tttggtgtac atacggaaca
     4501 aatcgcctgc aaaaatactc gcttcatccc cacccgttcc agcacgaatc tccaccatca
     4561 catttttggc atcttcaggg tctttcggaa tcaacataaa cttgatttct tcctctaatt
     4621 gaggcaaacg ttccttggct tcttcgagtt gcatcttggc catttctgtc atatccgcat
     4681 cacttccgtc agcaattatt tcgttagctt catcaatatt ggctaaaact aaaatgtatt
     4741 cttctctttt ctccaccaaa gctttgatgc ctttgtattc ctgattcagt agcacataac
     4801 gcttttgatc ggcaatcaca tcgggctgaa taatcaaatc cgaaatctcg tcgaaacgct
     4861 gctttacata ttgaagtcta tctaacatat tcttgttcct tttattggag tgcaaattta
     4921 caaaaaagaa ttgcagtttg caagattgct tttttgctgt ttctggacaa gcgaagcaaa
     4981 gcaattaagt caatcctttt attcgtcact tcgagtggtt tttaatagta attc
//
LOCUS       DAOSNN010000199         5011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_16883_length_5011_cov_6.208434, whole genome shotgun sequence.
ACCESSION   DAOSNN010000199 DAOSNN010000000
VERSION     DAOSNN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16883_length_5011_cov_6.208434"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..1530
                     /locus_tag="PLQ70_09755"
     CDS             409..1530
                     /locus_tag="PLQ70_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HQE34938.1"
                     /translation="MAINIEDAVSYHYDQFPPKNLEISNFINELLQATSAISRYDQML
                     KNMHNSEILLAPLRNQEAVISSRMEGTISTMDEILKYTADESGDASSIPKYRSDVIET
                     ILYQRALNNAQNAMIDGYNLSSSMIRTVHQQLLSFGRGTKKSPGKFKVEQNYLADRLK
                     NSILFVPISPEKLQDGLDLLFDYIENHPDPILVKTAVMHLEFEALHPFEDGNGRIGRM
                     LITLLLWSKGVISQPHFYISGYLEENKNLYIDMMRNVSQNGDWNQWCAFFLKAVETQA
                     NKNLEIAESIQKLYEDMKQVFVDKLSSKLAINALDYIFTNPIFKNNNFTSKSGIPPAT
                     AQRFTRILIEENLIVTLEEAAGRRSALYSFEPLMQLVRI"
     gene            1570..1734
                     /locus_tag="PLQ70_09760"
     CDS             1570..1734
                     /locus_tag="PLQ70_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34939.1"
                     /translation="MPKGPEKNTKNKALHTKLLNRKKAKLQEEKKEKAIRLKALVAKM
                     NQQKKADDLG"
     gene            1870..2433
                     /locus_tag="PLQ70_09765"
     CDS             1870..2433
                     /locus_tag="PLQ70_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34940.1"
                     /translation="MSTIFSQVEFSNDSIPKNAAKSSIGYFDLFAGGAFGYVDGFAGG
                     GSINAMYANVLFGFGYVMDNKQGGLEYVEEGTNLNYYKHYRLDSFPITVGFYRPFGGM
                     SLSASAGLSVLFYSEYVDVNTFSIFKSNATEIYSKTTLGFPYEFNLKFFSKKGGTDFG
                     YGLKLFGNFGKYNYVGLGLVLGLGNYK"
     gene            2508..3047
                     /locus_tag="PLQ70_09770"
     CDS             2508..3047
                     /locus_tag="PLQ70_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34941.1"
                     /translation="MEFGRIIISETAANSENPQDIINSNISVINLMREEKIDDDLIHE
                     DALMSYYLDYYASKYIQGNFAQFVHDSKWNKELNELIEEGLALIGAEKHLALFQENAK
                     KIRLMSNIKLDKFLKGKFEGVNPVRDMLINDTFFELDENLIALNANFLKSHPDTEVLS
                     VDEMFEVLEEFVGYEIKRE"
     gene            3254..>3797
                     /locus_tag="PLQ70_09775"
     CDS             3254..>3797
                     /locus_tag="PLQ70_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQE34942.1"
                     /translation="MLQIAFIRENQEKVIQALAKRNMDAKSVVEEVVKLDETRRATQV
                     ELDNTLAESNKLSKDIGELMKAGEKAKAAILKEKTVLLKEKSKDLSEKADAFAADLLE
                     KLYTLPNLPADIVPEGKSADDNLNVFQEGDIPVLQEGAQPHWELVKKYDIIDFELGNK
                     ITGAGFPVYKGKGAKLQRALI"
     assembly_gap    3798..3897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3898..4680
                     /gene="serS"
                     /locus_tag="PLQ70_09780"
     CDS             <3898..4680
                     /gene="serS"
                     /locus_tag="PLQ70_09780"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQE34943.1"
                     /translation="AGFPVYKGKGAKLQRALINYFLDKNTAAGYNEVQVPHMVNEASA
                     YGTGQLPDKEGQMYHDKTDDLYLIPTAEVPVTNLFRDVILAENELPILTTAYTPCFRR
                     EAGSYGAHVRGLNRLHQFDKVEIVRIEHPDKSYEALEGMVEHVKNILQELKLPYRILR
                     LCGGDMGFTSALTYDFEVYSTAQERWLEISSVSNFETFQANRLKLRFKGKDGKTQLAH
                     TLNGSSLALPRVLAGILENYQTAEGIVIPEVLRPYCGFDIID"
BASE COUNT         1708 a          759 c          927 g         1517 t
ORIGIN      
        1 gtatatcttt ttggattctg taacataatt cagtttaatt tgctgcaaaa ataaggaata
       61 gaattgagag ttttggaata agctagaaat gtagaattaa gtttatacta aaatgtataa
      121 atactttggt aatcaaagta gctaaataat ggttgacaaa tggttttgat gtaaatttgt
      181 gcaatacaaa tatgtttgtg aatcaaaaaa cacgaaaaat gattaacaaa ttggtttgat
      241 gttaatttgt gtaatgctaa aatttttgtc aatcaaaaaa actaaatatt gattaacaaa
      301 tgagtttgat gttaatttac atctaacaaa gttgtttgtc aatcaaaaac aacaaaaaat
      361 gattgacaat tgcggctaaa aaatatatct ttacaaaaaa atcacattat ggctattaat
      421 attgaagatg cagtttcata tcattatgat caatttccgc caaaaaattt agaaatatct
      481 aattttataa atgagctttt acaagcgaca tcagctattt ctaggtacga tcaaatgctt
      541 aaaaatatgc acaatagtga aattttatta gcgcctttgc gaaatcaaga agcagttata
      601 tcgtcaagaa tggaaggtac tatcagtacg atggatgaaa ttttaaaata cactgccgat
      661 gaaagtggtg atgctagtag tattccaaaa taccgttctg atgtaattga aaccattctg
      721 tatcaaagag ctttaaataa tgcacaaaat gcaatgatag atggttataa tttgtcttcg
      781 agtatgattc gaaccgtaca tcaacaactt ttatcttttg gtagaggaac taaaaaatca
      841 ccgggtaaat ttaaagttga acagaattat ttggctgatc gtcttaaaaa cagtatttta
      901 tttgtgccaa taagtcctga gaaactacaa gacggattag atcttttgtt tgactacata
      961 gaaaaccacc cagatcctat tttagtaaaa actgctgtga tgcatttaga atttgaagct
     1021 ttgcacccat ttgaagacgg aaacggaaga ataggtcgta tgctaattac gttgttattg
     1081 tggtccaaag gtgttatttc acaacctcac ttttatataa gtggttattt ggaagaaaat
     1141 aaaaaccttt acattgatat gatgaggaat gtttcgcaaa atggtgattg gaatcaatgg
     1201 tgtgcttttt ttctaaaagc tgtggaaacc caagcgaata aaaatttaga aattgcggaa
     1261 agtattcaga aactctatga ggatatgaaa caagtattcg tagataaatt gtcatctaaa
     1321 ttagctatta atgctcttga ttatattttt acaaatccta tttttaaaaa taataatttt
     1381 acttcaaaaa gtggtattcc tcccgcgaca gctcaacgtt tcacccgaat tttaatcgaa
     1441 gaaaacttaa tagttacttt ggaagaagct gcagggagac gatctgcact ttattcattt
     1501 gaaccattaa tgcaattggt taggatttag tacttaatta gaagaaataa tcatcgaagc
     1561 ttgtaaatta tgccaaaagg acctgaaaaa aacaccaaaa acaaggctct gcacaccaaa
     1621 ctgctcaatc ggaaaaaagc taaattacag gaggagaaaa aagaaaaagc catacgattg
     1681 aaagccttgg ttgccaaaat gaatcagcaa aagaaggcag atgatttagg gtaaatgcga
     1741 ttataataaa tgaataacaa ctttactgga gttgatattt tttatacctt taactatctg
     1801 tatgttatta acttaatcga aatcaatatg aaacagttct acttttgttg ttttttttcg
     1861 atattcacgg tgagtacaat tttttcacaa gttgaattct ctaatgatag catcccaaaa
     1921 aacgctgcca aatcttcaat tgggtatttt gatctctttg caggaggcgc ttttggttat
     1981 gtcgatggtt ttgcaggagg tggaagcatt aatgcaatgt atgctaatgt tttattcggt
     2041 tttggttatg ttatggataa caaacaagga ggcttggagt atgtggaaga agggacaaat
     2101 ttaaattatt ataaacacta tcgtttggat agttttccaa ttacggtagg tttttaccgt
     2161 ccttttggtg gtatgtcgtt gagtgcaagt gcaggattgt cagtgctttt ttattcagaa
     2221 tatgtagatg taaacacttt cagtattttt aaatccaatg ctaccgagat atattctaaa
     2281 accactttgg gttttcctta tgagtttaac ttaaaatttt tcagtaaaaa ggggggtaca
     2341 gattttggtt atggattaaa attgtttggc aattttggta aatacaatta tgtcggactc
     2401 ggattagtat taggtttagg aaactataaa taatataaaa agacaataaa tttcttttct
     2461 gcttttattt aacggaaatt tgccgcaaac cttcaaacac aaaccctatg gaattcggca
     2521 gaatcattat ctcggaaacc gcagccaaca gcgaaaatcc tcaagacatc atcaactcca
     2581 atatttcggt catcaacctg atgcgagagg aaaaaataga cgacgacttg attcacgaag
     2641 atgccctgat gagttattat ctggattatt atgcctcgaa atacattcag ggaaactttg
     2701 cccaatttgt gcatgattcc aaatggaaca aggaactcaa cgaattaatt gaagaaggat
     2761 tggccttgat tggagccgaa aaacacttgg ctttatttca agaaaatgcc aaaaaaatcc
     2821 gtttgatgag caacattaaa ttggataaat tcctgaaagg aaaattcgaa ggtgtcaacc
     2881 cggtacgaga tatgttgata aatgatactt tcttcgaatt ggatgaaaat ctaatcgcct
     2941 tgaatgctaa tttcctaaaa tctcatcccg ataccgaagt gctttccgtg gacgaaatgt
     3001 tcgaagtttt ggaagaattt gtgggttacg agattaagag ggaataggta attatttcac
     3061 aaagatacgc caagaaaaaa actaaagaac acgaagaatt ttgaataatt atagaaaact
     3121 tcgtgaatct ctgtgactct tttgcgaatc ttcgtgatat agcttttctt aaaaaacttt
     3181 tgtcttattt ctaccaaaca tttccttaaa tttgctcccg ttaatataaa gttagctttt
     3241 taaaaatcag gatatgttac aaattgcatt tattagagaa aatcaggaaa aagtaatcca
     3301 agctttggca aaaagaaata tggatgccaa aagcgttgtg gaagaagtgg taaaactaga
     3361 cgaaacccgt cgtgctaccc aagtagaatt agacaatact ttagccgaat ctaataaatt
     3421 gtccaaagac attggcgaat tgatgaaagc gggcgaaaaa gccaaagctg ccattctcaa
     3481 agaaaaaacc gttttgttga aagaaaaaag caaagactta tctgaaaaag cagatgcttt
     3541 tgcggccgat cttttagaga aactatacac tttaccgaac cttccagcag atattgttcc
     3601 tgaaggaaaa tcagcagacg acaatttgaa tgttttccaa gaaggcgata ttccagtttt
     3661 gcaagaaggc gcacagccac attgggaact ggtaaaaaaa tatgacatta tagatttcga
     3721 attaggaaac aaaataacag gcgcaggatt tccggtttac aaaggaaaag gagccaagtt
     3781 gcaacgtgcc ttaatcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca
     3901 ggatttccgg tttacaaagg aaaaggagcc aagttgcaac gtgccttaat caattatttc
     3961 ttggacaaaa acaccgctgc aggttacaat gaagtacagg tgccacatat ggtcaacgaa
     4021 gcttcggcat acggaacagg acaattgccg gacaaagaag gacaaatgta ccacgacaaa
     4081 accgatgatt tgtacttgat tccaacggca gaagttccag taacgaattt gtttcgtgac
     4141 gtgattttgg cagaaaacga attgccaatt ttaaccacgg cttacacacc ttgtttccgt
     4201 cgtgaagccg gttcttatgg agctcacgtt cgtggtttga accgtttgca ccaatttgac
     4261 aaagtcgaaa tcgtgcgtat cgagcatcct gacaagtctt acgaagcttt ggaaggaatg
     4321 gtcgaacacg taaaaaatat tctgcaagaa ctgaaattac cttatagaat attacgactt
     4381 tgtggcggcg atatgggttt cacatcggct ttgacttatg atttcgaagt gtattctacg
     4441 gcgcaagagc gttggttgga gatctcttct gtttccaatt tcgagacttt tcaagcgaat
     4501 cgtttgaaat tacgtttcaa aggtaaagac ggaaaaactc aattggcaca caccctaaac
     4561 ggaagttcat tggctttgcc aagagttcta gcaggaattt tggaaaacta ccagactgca
     4621 gaaggcattg taattcctga ggttttgcgt ccgtattgcg ggtttgatat tattgattaa
     4681 aaattcacca tataagtcat ttaaggtcat ttaagtgttt gtaatagttt atacaattat
     4741 ggattcagta acattttgga aaattattgt tcaaagtctt atcgcaataa cagttggtgt
     4801 atttatattg cgatatgcga agaaactgcc agaaagcaaa ttgtttagta taaaatttaa
     4861 aggttacgct gctggggttg gttttattct tcttggcatt atacatttac tcaatgaatt
     4921 ccatctgtgg taatacttaa atgaccttaa atgccttata tggttaaata attttcgaaa
     4981 atcaatgaaa aaactctttc tacatatcgc c
//
LOCUS       DAOSNN010000200         5009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_16898_length_5009_cov_5.739806, whole genome shotgun sequence.
ACCESSION   DAOSNN010000200 DAOSNN010000000
VERSION     DAOSNN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16898_length_5009_cov_5.739806"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLQ70_09785"
     CDS             complement(<1..183)
                     /locus_tag="PLQ70_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HQE34944.1"
                     /translation="MSIQEISLSGRKKLVSNKEMTFLEKMYLIAILKGLGITIKHFFR
                     KKATINYPEQVREFSPV"
     gene            complement(352..1404)
                     /gene="nuoH"
                     /locus_tag="PLQ70_09790"
     CDS             complement(352..1404)
                     /gene="nuoH"
                     /locus_tag="PLQ70_09790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQE34945.1"
                     /translation="MDSTFIIEKSVVILVVFGVTMLMAMYSTWAERKVAAYLQDRVGP
                     NRAGWGGLFQPLADGLKLFAKEEFAPNTPNQFLFRVGPGIAMATALMTSAVIPWGDRL
                     HLFGRDILLQATDINVGILYIFGIVSVGVYGIMIGGWASNNKFSLIGAIRAASQMVSY
                     EVAMGLSIVALLMMTGTLSLKEISEQQAGMNWNVFYQPISFLIFLICAFAETNRTPFD
                     LAECETELIGGYHTEYSSMRMGFYLFAEYANMFISSTILAVLFFGGYNYPGMSWVAEN
                     WGVNLANIIGMGALFVKLCGFIFFYMWVRWTIPRFRYDQLMHLGWRILIPLSIINIMI
                     TGIVILRGEILAYLGL"
     gene            complement(1397..2452)
                     /locus_tag="PLQ70_09795"
     CDS             complement(1397..2452)
                     /locus_tag="PLQ70_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQE34946.1"
                     /translation="MKVTIDGHEIEVEPGTTILQAARMIGGEVVPPAMCYYSKLKGSG
                     GKCRACLVEVAKGSEADPRPMPKLMASCVTSCQDGMEVNSKSSPRVQETRKAVTEFLL
                     INHPLDCPVCDQAGECDLQNLSFEHGKSESRYIEEKRTFEPENIGPNIQLHMNRCILC
                     YRCVMVADQLTDNRVHGVMDRGDHSNISTCISKAIDNEFSGNMIDVCPVGALTDKTFR
                     FKSRVWFNKPFNAHRECTTPGCCGKTTVWMFGNEIQRVTGRKDEYHEVEDFICNTCRF
                     DKKEVSDWVIEGPREFEKDSVINQNNYTQKLEKVIINTEETILKGREQDRKKISMAEI
                     DYNEKIDGNPLNAKNNG"
     gene            complement(2467..3441)
                     /locus_tag="PLQ70_09800"
     CDS             complement(2467..3441)
                     /locus_tag="PLQ70_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34947.1"
                     /translation="MKRNIQIILFLLLINLSFTSKVSAQIAIDNIEELSKIKGGTTYV
                     AMKDPNAEKSKEYVEIFKNNWTYTKLEFIKYTDITKYIAPGNSFLTLSGYVTDVQMMS
                     LYNNGIRPGIDYSHTHIFLQLWTVDDNYFKKNKEFNDKDRIQIARLELYTDFETLVRP
                     SNLYQNDYDADNHIRNWGLGILKNHIQNLNTYLNTGKKKSLFSDILNEKEIKKLASET
                     LYIPDYILVKFNKFTGDESAKLKEEELLEDYKFKYKMLSTKELNDKILSDTKGFYYLQ
                     YIKSSTNKYISVINSLTGEIIYSIYVPVSYNLKSDDLKDLSKIIQKIN"
     gene            complement(3452..4819)
                     /gene="nuoF"
                     /locus_tag="PLQ70_09805"
     CDS             complement(3452..4819)
                     /gene="nuoF"
                     /locus_tag="PLQ70_09805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQE34948.1"
                     /translation="MSQKILLDKVNIPGIKTYEVYRQNGGYASVEKALKTLTPDEVVE
                     EVKTSGLRGRGGAGFPAGMKWSFIDKKSGKPRHLVCNADESEPGTFKDRYLMEFIPHL
                     LIEGMITSSYALGANLSYIYIRGEYMWVYKILERAIAEAKAAGWLGKNILGTGYDLEL
                     YVQIGGGAYICGEETALIESLEGKRGNPRIKPPFPAVSGLWANPTVVNNVETIAAVPW
                     IINNSGADYAKIGVGRSTGTKLISASGHIKNPGVYEIELGLSVYEFMNSDEYLGGMSS
                     DRPLKAFVPGGSSVPVLPAHLIYKTANGDDRLMTYESLSDGGFATGSMLGSGGFIVYN
                     DTSCIVRNTWNFSRFYHHESCGQCSPCREGTGWMEKVLHRIENGHGREEDIDLLLSIQ
                     SKIEGNTICPLGDAAAWPVAAAIRHFREEFEYHIRFPEKIKNRDHFVAEPFESVKHLV
                     SKVTV"
     gene            complement(4821..>5009)
                     /locus_tag="PLQ70_09810"
     CDS             complement(4821..>5009)
                     /locus_tag="PLQ70_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQE34949.1"
                     /translation="GVGIGETTPDGLFEVRGVECLGACGYAPMMQLGDFYKEHLTKEK
                     VDQIIADCRDNKITLHDK"
BASE COUNT         1518 a         1043 c          729 g         1719 t
ORIGIN      
        1 aacaggacta aattcgcgca cctgttcagg ataattaatg gtagcctttt ttctaaaaaa
       61 gtgcttaata gtaatcccta agccttttaa tatagctata agatacattt tctctaaaaa
      121 agtcatctcc ttatttgaga ctagcttttt tctgccggaa agtgatattt cttgtattga
      181 catttatctt ttagatttta aattttaaat tcaggaactc ccgattcaaa acctgatttt
      241 cataatttct tttcttgtag cttaatcctg ctatccgcta tatcttttgt tttttaaaga
      301 aaaaaacaaa aggatgccgc ttctatcagg gctagggcat ttgttttcta tttacaaccc
      361 taaataagct aatatttctc ctctcaaaat tacaattcca gtaatcatga tattgataat
      421 cgatagtgga attaatattc tccaacccaa atgcatcaat tgatcgtatc taaatcttgg
      481 aatcgtccaa cgaacccaca tataaaagaa tatgaatcca cataatttta caaacaaggc
      541 tcccattcct atgatattag caagattcac tccccaattt tcagcaaccc aactcatgcc
      601 tggataatta tatcctccga agaataaaac agccaaaata gtagatgaaa taaacatatt
      661 ggcatattca gcaaataaat aaaaccccat tctcatagac gaatattccg tatgataccc
      721 accaattaat tcggtttcac attccgctaa gtcaaaagga gttcggtttg tttctgcaaa
      781 agcacaaatc aagaaaatca aaaaagaaat aggttgatag aaaacattcc aattcattcc
      841 agcttgttgt tctgaaattt cttttaaact caatgttccg gtcatcatca acaaggcaac
      901 gattgacaat cccatggcaa cttcataaga aaccatttga gaggctgcac gaatagcacc
      961 aatcaatgaa aatttattgt tagaagccca acctccaatc atgattccat aaactccaac
     1021 agatactata ccgaaaatat ataaaatacc aacattaata tccgtagctt gtagcaaaat
     1081 atctctacca aaaagatgca atcgatctcc ccaaggaatt acagcactag tcatcaaggc
     1141 agttgccata gcaataccag gacccactct aaataaaaat tggttgggtg tgtttggagc
     1201 aaattcttct ttagcaaaca attttaatcc atcggcaagc ggctgaaaca aacctcccca
     1261 acccgcacga ttaggaccaa ctctatcctg aagataagcc gcaacttttc gttctgccca
     1321 agtagaatac atcgccataa gcatggttac accaaataca accagaatta caacactttt
     1381 ttctataata aatgtactat ccattgttct ttgcgtttaa tgggttaccg tctatttttt
     1441 cgttgtaatc aatctctgcc atacttattt tttttcgatc ttgttctctt cctttaagaa
     1501 tcgtttcttc ggtgttgatg atcacttttt ctaatttttg agtataatta ttctggttga
     1561 taaccgaatc tttttcaaat tctcttggcc cttcaattac ccaatccgaa acctcttttt
     1621 tgtcaaaacg acaagtattg caaatgaaat cttctacctc gtggtattcg tcttttcttc
     1681 ctgtaactct ctgaatttcg tttccaaaca tccaaacagt tgtttttccg caacatcctg
     1741 gagtggtgca ttctctgtga gcattaaatg gtttgttgaa ccaaactctc gatttgaatc
     1801 taaaagtttt gtcggtcaaa gcaccaacag gacaaacatc aatcatattt cctgaaaact
     1861 cgttgtcaat cgctttagaa atacaagtcg aaatattcga atgatcgcct ctatccatca
     1921 ctccgtgtac tcggttgtct gtcaattgat ccgcaaccat tacacaacgg tagcacagaa
     1981 tacaacgatt catatgcaat tgaatattcg ggccaatatt ttcgggttcg aacgttcttt
     2041 tttcttcaat ataacgagat tctgattttc cgtgttcgaa actcaagttc tgcaaatcgc
     2101 attctcccgc ttgatcacaa actggacaat ccaaagggtg attaatcagc aagaattccg
     2161 tcactgcttt tcttgtttct tgtactcttg gtgaagattt gctgtttact tccattccat
     2221 cctgacaact tgttacacaa gaagccatta atttaggcat tggtcttggg tcggcttcac
     2281 ttcctttggc tacttctacc aaacaagctc ggcattttcc accgctgccc tttagcttag
     2341 aataatagca catcgctggc ggaactactt ctcctccaat cattctggct gcttgcagga
     2401 ttgttgttcc tggttctact tctatctcgt gaccgtctat tgttactttc attatgttga
     2461 aaatagttaa ttaatttttt gaattatttt tgacaaatct ttcaaatcat cggatttaag
     2521 attgtatgat acaggcacat atatagaata tattatttcg cctgttaatg aatttattac
     2581 gctaatgtat ttattggtgc tactttttat atattgtaaa taataaaatc cttttgtgtc
     2641 acttaaaatt ttatcattca attcttttgt agatagcatt ttatatttga atttataatc
     2701 ttctaaaagc tcttcttctt ttaatttagc tgattcgtca ccagtaaatt tattgaattt
     2761 aactagaata taatctggta tatacaatgt ttcactagct aattttttta tttccttttc
     2821 gtttaagatg tctgaaaaca agcttttttt ctttccagtg ttcaaataag tattcagatt
     2881 ttgaatgtga tttttaagaa ttcctagtcc ccaattgcga atatgattat cagcatcata
     2941 atcattttga tagagattgc ttggtcttac aagggtttca aaatctgtat ataattccaa
     3001 acgagcgatt tgaattctat ctttatcgtt aaactctttg tttttcttaa agtaattatc
     3061 gtccacagtc caaagttgta gaaagatgtg agtgtgagag taatcaatac ccggacgtat
     3121 tccattgtta tacaaactca tcatttgtac atctgtaaca tatccagata gggtcaaaaa
     3181 agaattccca ggagcaatat atttggtgat atctgtatat ttaataaatt caagtttagt
     3241 ataagtccag ttatttttaa atatttctac atattcttta gatttttcgg cattagggtc
     3301 tttcattgca acgtatgtag ttcccccttt aattttagaa agttcttcaa tattatcaat
     3361 tgcaatttga gcacttactt ttgaagtaaa tgacaaattt atgagcaata aaaacaatat
     3421 aatttgaata tttcttttca ttatttgcct attataccgt tactttagaa actaaatgct
     3481 ttacactttc aaaaggttcg gcaacaaaat gatctctgtt ttttattttt tctgggaaac
     3541 ggatatggta ttcaaactcc tctctaaagt gacgaatggc tgctgccact ggccaagctg
     3601 ctgcatcacc caaaggacaa atcgtgtttc cttcaatttt gctttgaatg cttaaaagca
     3661 aatcgatatc ttcttcacga ccgtgaccgt tttctattct atgcaatact ttttccatcc
     3721 aaccggttcc ttcacgacaa ggtgaacatt gtccgcaaga ttcgtggtgg taaaaacgag
     3781 agaaattcca tgtgtttcta acaatacaag atgtgtcgtt gtaaacaata aatcctcctg
     3841 aacccaacat tgaacccgtt gcaaaaccac cgtcgctcaa actttcgtaa gtcatcaaac
     3901 ggtcgtcgcc attggctgtt ttataaatta aatgagctgg taaaactgga actgaacttc
     3961 ctccaggaac aaacgctttt aaaggtctgt ctgaactcat tccgcccaaa tattcatcag
     4021 aattcataaa ctcgtaaacg cttaaaccca attcaatttc ataaactccc gggtttttga
     4081 tgtgtcctga agccgaaatt aatttggttc ctgttgatct tcccacacca attttggcat
     4141 aatcagcacc tgaattgttt ataatccaag gcactgcagc aatcgtttca acattgttta
     4201 caaccgttgg attcgcccaa agtccagaaa ctgctggaaa tggaggtttg atacgaggat
     4261 ttcctctttt tccttctaat gattcaatta atgcggtttc ttctccacaa atgtaagcac
     4321 caccaccaat ttgaacatac aattctaaat cgtatcctgt tcctaatata ttttttccta
     4381 accaacctgc agcttttgct tcggcaatgg cacgttccaa aattttgtac acccacatat
     4441 attcaccacg aatgtaaatg taagataggt tcgcacccaa agcatagctg gaagtaatca
     4501 ttccttcaat taataaatga ggaataaatt ccatcaaata tctgtctttg aaagttcccg
     4561 gctctgattc gtcagcgttg caaactaaat gtcttggttt tcctgatttt ttgtcaataa
     4621 aactccattt cattcccgct gggaaaccag caccaccacg accacgtaat cctgaagttt
     4681 ttacttcttc aaccacttcg tctggtgtta acgttttcaa ggctttttct actgaagcat
     4741 aaccaccatt ttggcgatac acttcatagg ttttaatccc tggaatattt actttgtcta
     4801 ataatatttt ttgtgacata ttatttatcg tgtaacgtaa ttttattatc tctacaatcg
     4861 gctataatct gatctacttt ttctttggtc aagtgttctt tatagaaatc acccaattgc
     4921 atcattggag cataaccaca agcgcccaaa cattctacgc ctcttacttc aaaaagtccg
     4981 tctggagtag tttcgccaat tccaacgcc
//
LOCUS       DAOSNN010000201         5003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_16918_length_5003_cov_5.077203, whole genome shotgun sequence.
ACCESSION   DAOSNN010000201 DAOSNN010000000
VERSION     DAOSNN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16918_length_5003_cov_5.077203"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..502)
                     /locus_tag="PLQ70_09815"
     CDS             complement(5..502)
                     /locus_tag="PLQ70_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34950.1"
                     /translation="MDANNEIRLRLRFYKDVPGNIESIRQKFENYKVNCTEDCHLKIK
                     HNHIWMNMPDAKREYWSPHLHLELESKGNNETHIRGLFGPEPTLWTLFMFLHFMVAGI
                     FVIFSAIAYSNWVLKQPTTMDFIVMLLMVIVWFLLYFIAKQIRFKGNGQMNELEGKFL
                     EILES"
     gene            609..1919
                     /gene="der"
                     /locus_tag="PLQ70_09820"
     CDS             609..1919
                     /gene="der"
                     /locus_tag="PLQ70_09820"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQE34951.1"
                     /translation="MNNIVAIVGRPNVGKSTLFNRLIQRREAIVDSVSGVTRDRNYGK
                     SEWNGKEFSVIDTGGYVRGSDDVFEGEIRKQVELAIDEADVIIFVVDVEEGITPMDET
                     VARLLRKVTKPVLLAVNKVDNAMREKDAIEFYNLGLGDYFTFASISGSGTGDLLDALI
                     EAFPVKPEPAKEEVVLPRFAVVGRPNAGKSSFINALIGKDRFMVTDIAGTTRDAIDTK
                     FDRFGFEFNLVDTAGIRRKAKVKEDLEFYSVMRSVRAIEHADICILVIDATRGFEGQD
                     QSIFWLAEKNRKGVVILVNKWDLVEKDTMSTRDYEEKIRKELMPFTDVPILFVSALTK
                     QRLLKALEATVKVYENRQQRIATSKFNEYMLKVIEAYPPPATKGKYVKIKYCMQLPTA
                     TPQFVFFANMPQYVKEQYKRYLENKIRENWDFSGVPIDIYIREK"
     gene            2041..4842
                     /locus_tag="PLQ70_09825"
     CDS             2041..4842
                     /locus_tag="PLQ70_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQE34952.1"
                     /translation="MAKIYSFLILFLFVYAANAQNEVIIKGTVYDINTQVPLELATVY
                     FSNAKDSTIIEYANTDKNGAFKIATKKYDKAILLKVNYMGYQTYTEEQNGLLESKDFG
                     KIYLLHNTSELKEVVINSAPPIRIKKDTLEFNAASFKVRPDANLEVLLKQLPGFEVDS
                     DGKITVNGKEVNQFLVNGKAFFDKDGAIALKNLPADIINKVQVSDYKTKKEELSKQES
                     SSDYSSINLTIDEKKNKGYFGKFLAGYGTDDRYETSMILNFFKDKQKISILASANNIN
                     STGFTQDEVFDSMGGGRNANGNARATGTGRGITESNMVGFNYNDEWSKKFEASGSYNF
                     DNAVNKNESKSNQANFLPTSTFFTESNSKARSENTGNKANFEFEYKINPTMRLVVTPR
                     VDQTRSNSNSASSSSSKDENDALLNESNGISQRESTATNFANTINFNKTFEKRSRNLS
                     FVFSNNNSDNESDALNISKTIFYQGSKPNDERNQNSKGNNNYGAYSADIEYTEPITDS
                     LRVRIGADFDWQNAMNDSKTYDFDAASQTYSTLNDLQTNYTSYTRNSITPKVGLSFEK
                     NNFTFNLNSSTSIIDFDNHSLYLNKITDLNQKYVLPFGRAQIRYRVDRSKFFTLRYDY
                     SSSLPSSTQLMPVANLSNPLSTIIGNPNLNPTEKNSFNMNYRNFDVRTRSGYTLFMRG
                     DFFNNEIVSTSVYDQSGKRTTTYRNISGTYTTSVGGNWNQTIKREAHVLRYGLSINGS
                     YSLDKGFTNTVLYTSKRLGATPRVYFAYDYGELFTISPSYSLSYNDSKYTNSSLKASS
                     NVVHRINIQTTNYWPKNWVFGNDFGYTYNSNISNNFKKDFYLWNTSLSYAFLAKKMTL
                     KVKVYDVLNQNQSATRTISPTTIRDEENTVLKRYAMFSLAYKLGNFGGREGRSRNARG
                     GGGNGGEGNGSGRGMDNMD"
BASE COUNT         1743 a          794 c          979 g         1487 t
ORIGIN      
        1 tagtttagga ttccaaaatc tccaaaaact tcccttccaa ttcattcatt tgtccgttgc
       61 ctttgaatct tatttgtttg gcgatgaaat agagcaaaaa ccaaacgata accattagca
      121 acatcacgat gaaatccatt gtggtgggtt gtttcaagac ccagttagag taggcgatgg
      181 cactaaaaat gacaaaaatt cccgccacca taaaatgcaa aaacataaaa agcgtccata
      241 aagtgggttc tgggccaaac aatccccgaa tgtgggtttc gttgttgcct ttggattcca
      301 attctagatg caaatggggc gaccaatatt cgcgtttagc atcgggcata ttcatccaaa
      361 tgtggttgtg cttgattttt agatggcaat cttcggtgca atttacttta taattttcaa
      421 atttttgacg aatggattct atgtttccag gaacgtcttt gtaaaaacgc aagcgaagtc
      481 ttatttcatt attggcatcc ataatggtaa tttttttggt tcgaaaagat taataacgat
      541 gcaaaatagt aaaaaaatgg atagtatggt atttaacact acctttgcaa aatatttgaa
      601 tttatattat gaataatatt gttgcgatag taggaagacc taatgtgggg aaatcgaccc
      661 tttttaatag gctgatacaa agaagagaag ctattgtaga ttcagtatct ggggtgaccc
      721 gagacagaaa ctatggtaaa agcgagtgga acggaaaaga gttttccgtg attgatacgg
      781 gaggatacgt tcgcggaagt gacgacgtat tcgaaggtga aatccgcaaa caagtagaat
      841 tggccatcga cgaagccgac gtaattatct ttgtggtaga tgtggaagaa ggcatcacgc
      901 caatggatga gaccgttgcc cgattgttac gcaaagtgac aaaacccgtt ttgttggccg
      961 taaacaaagt agataatgcg atgcgtgaaa aagacgccat cgaattttat aatctaggtt
     1021 tgggtgatta tttcaccttt gccagtattt caggaagtgg gacaggcgat ttgttggatg
     1081 ctttgattga ggcattccca gtaaaaccag aacccgcaaa agaagaagtg gttttgccac
     1141 gttttgcggt tgtaggtcgt ccaaatgctg gaaaatctag tttcatcaac gccttgattg
     1201 gtaaggatcg ttttatggtt acagatattg cgggaacgac tcgtgatgcg attgatacca
     1261 aattcgaccg ttttggtttc gaattcaact tggtcgatac ggctggaatt cgccgtaaag
     1321 ccaaagtgaa agaagattta gaattttact ccgtgatgcg ttcggttcgt gccattgaac
     1381 acgctgatat ttgtattttg gtaatcgatg ccactcgtgg attcgaaggt caagaccaaa
     1441 gtattttctg gttggccgaa aaaaaccgta aaggagtcgt gattttggtc aacaaatggg
     1501 atttggtcga aaaagacacg atgtcaaccc gagattacga agaaaaaatc cgaaaagaat
     1561 tgatgccatt taccgatgtg cccattcttt ttgtttcggc attgaccaaa caacgtttgt
     1621 tgaaagcact ggaagcaact gttaaagttt atgaaaacag acaacaacgc attgccacat
     1681 caaagttcaa cgaatatatg ttgaaggtaa ttgaagctta tccgccgcca gcgaccaaag
     1741 ggaaatatgt aaaaattaaa tattgtatgc agttgccaac cgcgacacca cagtttgtgt
     1801 tttttgccaa tatgccgcaa tatgttaaag agcaatacaa aagatatctt gaaaataaga
     1861 ttagagaaaa ttgggacttc tcgggagtgc caatcgatat ttatattaga gaaaaataaa
     1921 atgaaatccc gagcaatcgg gattttttta tccctttttt agattttgga aataaatgta
     1981 gaaattacaa ttaaaccttt agaaatttat ttagtctaaa cactttataa aaattattca
     2041 atggctaaaa tctattcttt tttaattctc tttctatttg tttatgctgc caatgcgcaa
     2101 aacgaagtaa tcatcaaggg aacagtttat gacatcaata cacaagttcc gctagagtta
     2161 gcgacggtct atttttcgaa tgccaaagat tcaaccatta ttgagtatgc caatactgat
     2221 aaaaatggag cttttaaaat tgccaccaaa aaatatgata aagccattct tttgaaggtc
     2281 aattatatgg gctatcagac atatactgaa gagcaaaatg ggcttttgga aagcaaggat
     2341 tttggaaaaa tatatttgtt acacaataca agcgaattga aagaagttgt aatcaacagt
     2401 gcaccgccaa ttcgaataaa aaaagacact ttagagttta atgcggcttc tttcaaggtg
     2461 cgtccagatg ccaatctaga agttttgttg aaacaattgc cgggatttga agtggatagt
     2521 gacggaaaaa taactgtaaa tggaaaagaa gtcaaccagt ttttagtaaa cggtaaagct
     2581 tttttcgaca aagacggagc catcgcttta aaaaacttgc cagcagatat tatcaacaaa
     2641 gtgcaagtat cggattataa aaccaaaaaa gaggaattat ccaaacaaga atctagttct
     2701 gattattcca gcatcaattt gaccattgac gaaaagaaaa acaaaggcta ttttgggaaa
     2761 ttcttagcgg gatacggaac tgatgatcgc tatgaaacaa gtatgatttt gaatttcttt
     2821 aaggataaac aaaaaataag cattttggct tcagccaata atatcaattc aactggattt
     2881 acccaagacg aagtttttga tagtatggga ggaggaagaa atgctaatgg gaatgcccga
     2941 gcaacgggaa caggaagggg aattaccgag tctaatatgg ttggatttaa ttataacgat
     3001 gaatggtcga aaaaatttga agcatcaggg agttataatt ttgataatgc ggtaaataaa
     3061 aatgaaagta aatccaatca ggccaatttc ttgcctacaa gtactttttt tacagaatct
     3121 aattcgaaag caagaagcga aaacactggg aataaagcca attttgaatt tgaatataaa
     3181 attaatccaa ctatgaggct ggttgtaact ccaagagtag atcaaacccg ttctaatagt
     3241 aattcggcat cttctagctc ttcaaaagat gagaatgacg ctttattgaa cgaaagtaat
     3301 ggaatatcgc agagagaaag tacggccaca aattttgcaa acacaataaa ttttaataaa
     3361 actttcgaaa agagatctcg taatttaagt tttgtcttta gtaataataa ttctgataac
     3421 gaatcggatg cgctcaatat ttctaaaact attttttatc aagggtctaa acctaacgat
     3481 gaacgaaatc aaaatagcaa aggaaataat aattatggtg cttattcggc cgatattgaa
     3541 tatacagagc ctattacaga ttcgttgcgt gttcgaattg gtgctgattt tgattggcag
     3601 aacgccatga acgacagcaa aacttatgat tttgatgctg cttctcaaac atattctact
     3661 ctaaatgatt tgcaaacaaa ttatacttcc tatacacgaa attccattac accaaaagtg
     3721 ggacttagtt tcgaaaaaaa taattttaca tttaacttga attcgagtac ttctattata
     3781 gattttgaca atcactcttt ataccttaat aaaattaccg atttgaacca gaaatatgtt
     3841 ttaccttttg gaagagcaca aatcagatat agggtagatc gctctaaatt ttttacgtta
     3901 agatatgatt attctagcag tcttccttca tctactcaat tgatgcctgt tgcgaatttg
     3961 tctaatccgt tgagcaccat aataggaaat ccaaacttga atccaaccga aaaaaatagt
     4021 ttcaatatga attataggaa tttcgatgtg cgaactcgtt caggttacac cttgtttatg
     4081 agaggagatt ttttcaacaa cgaaatagtt tcaacttcgg tttatgatca aagtggtaaa
     4141 aggaccacca cttatagaaa catttctgga acctatacca cttcagttgg tggaaactgg
     4201 aatcaaacta taaaaaggga agctcacgtt ttgcgatacg gactaagtat aaatgggagt
     4261 tattctttgg acaaaggatt tacaaatacc gttttgtaca cctcaaaaag actaggggct
     4321 acaccgagag tttattttgc ttatgattac ggtgagttgt ttaccatttc accttcctat
     4381 agtttgtcgt ataacgactc taaatatacc aactcatctt taaaagcgag ttcaaatgtt
     4441 gttcatagaa ttaatattca aacgaccaac tattggccta aaaattgggt ctttggaaat
     4501 gattttggat atacttacaa ttcgaatatc tcaaataatt tcaaaaaaga tttttattta
     4561 tggaatacga gtttgtccta tgcttttttg gctaaaaaaa tgacattgaa agtaaaagtt
     4621 tatgacgtct taaatcaaaa tcaaagtgct actagaacca tatcgccaac cacaattcgt
     4681 gatgaagaaa acacggtttt aaagcgatat gcaatgtttt ctttggctta taaattaggg
     4741 aattttggtg ggagagaagg tcgttctaga aatgctagag gcggtggcgg taatggaggc
     4801 gaaggaaatg gaagcggaag aggaatggat aatatggatt aattgtttta aataatttat
     4861 aattaatcaa ttaaaccatt ctgtaattgt atagtcatat aaatctaaac ttaaaaaaaa
     4921 ataaaaatga aaaaattaat tatcgcagca ttataggtta taggtatata gactattgca
     4981 caagaaaaca aaccagaaaa aaa
//
LOCUS       DAOSNN010000202         4996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_16947_length_4996_cov_4.998988, whole genome shotgun sequence.
ACCESSION   DAOSNN010000202 DAOSNN010000000
VERSION     DAOSNN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16947_length_4996_cov_4.998988"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /gene="hisG"
                     /locus_tag="PLQ70_09830"
     CDS             <1..807
                     /gene="hisG"
                     /locus_tag="PLQ70_09830"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQE34953.1"
                     /translation="SIQILKDCGISINNGNDQLKAEATNFPLEVLYLRNSDIPQYLID
                     GVVDIAIVGDNLLVEKGKNIKVIQKLGFSKCKVSVAVPKAFEYKSIKDLEGMRIATSY
                     PNTVIEYFAKFGMTVDIHQISGSVEIAPNIGLADGIVDIVSSGSTLFKNNLKEVEVIF
                     KSEAVLAVSPKVTPEVQSIIDTLQFRIESVLRARKSKYILMNVPNDKIEAIGQILPVL
                     KSLTVMPLAQEGWSSVHSVIDKDTFWDVIDQLKEAGAEGILVCPIEKMVL"
     gene            840..2132
                     /gene="hisD"
                     /locus_tag="PLQ70_09835"
     CDS             840..2132
                     /gene="hisD"
                     /locus_tag="PLQ70_09835"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQE34954.1"
                     /translation="MNKIFNPKPETWSEILKRPTQTIDDIEVTVKEIFKEIQKKGDEA
                     VAKYTSLFDGVLFENIEVAESEIEQAIATIPDELKTAIQLAKSNIEKFHSAQKTTKIE
                     VETIEGVNCWQEKRPIQKIGLYIPGGTAPLFSTVLMLAVPANIAGCNEIVLCSPPDKS
                     GNINPAILYAANLCGVTKILKVGGIQAIAGMTFGTKSIPKVYKIFGPGNQFVTVAKQL
                     ATQYGVAIDMPAGPSELLIVADDTAVPAFIASDLLSQAEHGTDSQVILVSTSKVLIDE
                     VEKEIQSQLEVLPRKAIAEKAIANSKLIYVENDKTALELIDEYGPEHFIICTKDEDFY
                     VQNIGNAGSVFIGNYTPESAGDYASGTNHTLPTNGYAKNYSGVNLDSFTKSITFQKIS
                     EKGIQNIGNAIEVMAEAEGLQAHKNAVSLRLKSLKIEK"
     gene            2198..3241
                     /gene="hisC"
                     /locus_tag="PLQ70_09840"
     CDS             2198..3241
                     /gene="hisC"
                     /locus_tag="PLQ70_09840"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010178536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQE34955.1"
                     /translation="MNVFNINNLVRENVKSMKPYSSARDEFEDFDTADMIFLDANENP
                     FQNGVNRYPDPQQSSVKVVLAKQKNVNPNQILLGNGSDEVLDLLFRAFCEPKIDNVIT
                     LPPTYGMYSVLSNLNNIENREVLLSTDFQPQVNKILDAVDANSKIIFLCSPNNPTGNS
                     FTTESVTTLLEKFNGFIVIDEAYIDFSDKASWLEKLNQYPNLIITQTLSKAYGLAGIR
                     LGICYASAEVIAVLNKIKPPYNVNELTQQRALERLKDENKIKDEITSILKERESLLKV
                     LNEVDFVEKIYPTEANFILIKVDDANKRYDELIAKGIVIRNRTTQPLCENTLRLTIGT
                     EVENKKLIEALKA"
     gene            3245..4381
                     /gene="hisB"
                     /locus_tag="PLQ70_09845"
     CDS             3245..4381
                     /gene="hisB"
                     /locus_tag="PLQ70_09845"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HQE34956.1"
                     /translation="MKKVLFIDRDGTMVLEPNDYQLDSFEKLEFYPKAFQYLGKIATE
                     LDFELVMVTNQDGLGTDSHPEANFWPVHNLVMKAFENEGVVFSEVFIDKTFPHENVPT
                     RKPGTAMLTKYINNPEYDLENSFVIGDRITDVELAKNLGSKAIFISNDEELGSDEISS
                     KRHELDAVIALQTTEWKTIYEFLKLEERTASITRKTHETDIYINLNLDGTGKSKIETG
                     IAFFDHMLDQIARHGQMDLEIIVKGDLEVDEHHTIEDTAIALGEVFAKALGNKLGIER
                     YGFCLPMDDCLSQVAIDFGGRNWLVWETEFKREMVGKMPTEMFYHFFKSFSDGAKANI
                     NIKAEGINEHHKIEAIFKAFAKAIKVAVKRDTEKMILPSTKGML"
     gene            4393..4740
                     /locus_tag="PLQ70_09850"
     CDS             4393..4740
                     /locus_tag="PLQ70_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQE34957.1"
                     /translation="MDAQKFAKEWIESWNSHDLENIMKHYSEDIEITTPMIKLAGGIE
                     SGSLFGKESVKDYWNKALTKFPDLHFELIEVTSSVNSVALYYKSIMDKMAIEVMFFDD
                     KGLVNKMIAHYTN"
     gene            4741..>4996
                     /locus_tag="PLQ70_09855"
     CDS             4741..>4996
                     /locus_tag="PLQ70_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQE34958.1"
                     /translation="MKIVIINYGAGNIQSIMFAIERLGYKAVLSNNPDEIKAADKVIF
                     PGVGEASYAMKMLQESGLDVLIPTLKQPVFGICLGMQLMCN"
BASE COUNT         1740 a          822 c         1023 g         1411 t
ORIGIN      
        1 agcattcaaa ttcttaaaga ttgtggtatt tccatcaaca acggaaatga ccaactcaaa
       61 gccgaagcca ccaattttcc tttggaagtt ttgtatttga gaaactcgga tattcctcaa
      121 tatcttattg acggagtggt agatattgcc attgtgggcg ataatctttt agtcgaaaaa
      181 ggaaaaaaca ttaaagtaat ccagaagtta ggattttcta aatgcaaagt ttccgtagcc
      241 gttccaaaag ctttcgaata caaatccata aaagacttgg aaggaatgcg tatcgccact
      301 tcttatccaa acaccgttat cgaatatttt gccaaattcg gaatgaccgt tgacattcac
      361 caaatctctg gttcggttga aatagcacca aacatcggtc ttgccgacgg aatcgtggac
      421 atcgtttcca gcggaagcac tttattcaaa aataatttga aagaagtgga agtgattttc
      481 aaaagtgaag ccgttttggc cgtttcccca aaagtgactc cggaagttca atctataatt
      541 gatacacttc aattcagaat cgaatcggtt ttgagagcca gaaaatcaaa atacatcttg
      601 atgaacgttc ccaacgacaa aattgaagcc attggccaaa ttctgccggt tttgaaaagt
      661 cttaccgtaa tgccattggc acaagaaggc tggagcagcg ttcattccgt aatcgacaaa
      721 gacacttttt gggatgtcat cgaccaattg aaagaagccg gagccgaagg aattttagtt
      781 tgtccaatcg agaaaatggt actttaaaaa tattggaatt tatcttaatg atataaaaaa
      841 tgaacaaaat attcaatcca aaacccgaaa cttggtctga aatcctgaaa agaccaactc
      901 aaaccataga cgatattgaa gttaccgtga aagaaatttt caaggaaatt caaaaaaaag
      961 gggatgaggc tgttgccaaa tacacttcac tgttcgacgg tgttttattt gaaaacatag
     1021 aagttgctga atcggaaata gagcaagcca ttgcaactat tcctgatgaa ttaaaaactg
     1081 ccattcaatt ggcaaaatcc aacatagaaa aattccattc ggctcaaaaa acgactaaaa
     1141 ttgaagtaga aacaatcgaa ggagtaaact gttggcagga aaaacgccca attcaaaaga
     1201 taggtttgta cattccagga ggaacagcgc ctttgttttc aacagtttta atgcttgccg
     1261 ttccagccaa tattgcaggt tgcaacgaaa ttgttttgtg ttcgccacca gacaaaagcg
     1321 gaaacattaa tccagcgatt ttatatgccg ccaatttatg tggtgtgacc aaaatcttga
     1381 aagtgggcgg tatccaagcg attgcaggaa tgactttcgg aacaaaatca attccaaaag
     1441 tgtacaagat ttttggacct ggaaatcagt ttgtgactgt agccaaacaa ttggcaactc
     1501 aatatggtgt ggcaatcgat atgccggccg gtccttctga attgttgatt gtggccgatg
     1561 atactgccgt tcccgctttc atcgcttccg atttgttgtc gcaagcggaa cacggaaccg
     1621 acagtcaggt gattttggtt tctacttcca aagtattgat tgatgaagtg gaaaaagaaa
     1681 ttcaatccca attggaagtt ttgccaagaa aagccattgc cgaaaaagcc attgccaatt
     1741 cgaaattgat ttatgtcgaa aatgacaaaa ccgctttgga attaattgac gaatatggtc
     1801 cagaacattt tattatttgt actaaagacg aagattttta tgtacagaat ataggaaatg
     1861 caggttcggt tttcattgga aattatacac cggaaagtgc tggagattat gcttcaggaa
     1921 ccaatcacac cttgccaacc aacggatatg ccaagaatta cagcggcgtg aatctggata
     1981 gttttaccaa atcgataact ttccaaaaaa tatctgaaaa agggattcaa aacattggca
     2041 acgccattga agttatggca gaagccgaag gattgcaggc acacaaaaat gcggttagtt
     2101 tgagattgaa gagtttaaaa attgaaaaat aaaatcagct tttatccgtg tttttgcttt
     2161 agcaaatccg ttttatcagc gtccaaaata taatgtaatg aatgttttta atataaataa
     2221 tttagtccgt gaaaacgtca agtcgatgaa gccttattct tcggctcgtg acgaatttga
     2281 ggattttgac acagcggata tgattttctt ggatgccaat gaaaatccat tccaaaatgg
     2341 agtaaaccgt tatccagatc cgcaacaaag cagcgtgaaa gtggttttgg cgaagcagaa
     2401 aaacgtaaat ccaaatcaaa ttttattagg taatggaagt gatgaagtgt tggatttgct
     2461 atttagagct ttttgcgagc ccaaaattga caatgtaatc actttgcctc caacctacgg
     2521 gatgtatagt gttctatcaa atctgaataa tattgaaaac agagaagttt tgctttcgac
     2581 tgattttcag ccacaggtaa acaagatttt agatgctgtt gatgctaatt ctaaaattat
     2641 ctttttatgt tcgccaaata atccaacagg aaattctttt acaacagaaa gtgtgaccac
     2701 tttattggaa aaattcaatg gatttattgt gattgacgaa gcttatattg acttttcaga
     2761 taaagcaagc tggttagaaa aattgaatca atatccgaat ttgattatca cacaaacttt
     2821 gtcaaaagcg tatggtttag cgggaattcg tttgggaatt tgctatgctt cagccgaagt
     2881 aattgcggtt ttgaataaaa tcaaaccgcc ttataacgtg aatgaattga cacaacaaag
     2941 agctttggaa cgtttgaaag atgaaaataa aattaaagat gaaataactt caattttaaa
     3001 ggaaagagag tctttactta aagtgttgaa tgaagttgat tttgtagaaa aaatctaccc
     3061 aacagaagcg aattttatct tgataaaagt agatgatgcc aataaaagat acgatgaatt
     3121 gattgccaaa ggaatcgtga ttcgtaacag aacaacacaa ccactttgtg aaaacacctt
     3181 gcgtttgact attggaactg aggttgaaaa taaaaaatta attgaagcat taaaagctta
     3241 aaaaatgaaa aaagtacttt ttatagatcg tgatggaacg atggttttag aaccaaacga
     3301 ctatcaatta gacagtttcg aaaaattgga attctatcca aaagcttttc aatatctggg
     3361 aaaaattgct actgaattgg attttgagtt ggtgatggta accaatcagg atggtttggg
     3421 aacggattct catccggaag ctaacttttg gccggtgcat aatttggtga tgaaagcttt
     3481 cgaaaatgaa ggagttgttt tcagcgaagt ttttattgac aaaacatttc ctcacgaaaa
     3541 cgtaccaacc cgcaagccgg gaacggctat gttgacgaaa tacatcaaca atccagaata
     3601 tgatttagaa aattcattcg tgattggcga cagaataacc gacgtggaat tggcgaaaaa
     3661 tttgggttca aaagcgattt ttatcagcaa tgatgaagaa ttgggaagcg atgaaatttc
     3721 gtctaaaaga cacgaattgg atgccgttat tgctttgcaa accacggaat ggaaaacgat
     3781 ttacgagttt ttgaaattgg aagaacgcac tgcttcaatt accagaaaaa cgcacgaaac
     3841 cgatatttac atcaacctga atcttgacgg aaccgggaaa agcaaaatcg aaacaggaat
     3901 tgcttttttc gaccatatgc tagatcaaat cgctcgtcac ggacaaatgg acttggaaat
     3961 cattgtaaaa ggagatttgg aagtcgatga acaccacaca attgaagaca cggcgattgc
     4021 cttgggggaa gtttttgcta aagctttagg aaataaatta ggaatagagc gttacggttt
     4081 ttgtttgcca atggatgatt gtttgtcgca agtagccatt gattttggag gcagaaactg
     4141 gttggtttgg gaaaccgaat tcaaacgcga aatggtgggt aaaatgccaa ctgaaatgtt
     4201 ctatcatttc ttcaaatcct tttcggatgg agccaaagcc aacatcaaca tcaaagccga
     4261 aggaatcaac gaacaccaca aaattgaagc tattttcaaa gcttttgcga aagcgataaa
     4321 agtagctgta aaacgcgaca cggagaaaat gattttgcca tcaacaaaag gaatgcttta
     4381 atagtaacaa ttatggatgc tcaaaagttt gctaaagaat ggatagaatc gtggaattct
     4441 cacgatttgg agaacattat gaaacactat tccgaagata ttgaaatcac tacaccaatg
     4501 ataaaattgg ctggaggaat tgaaagtggt tctctttttg gaaaagaatc ggttaaagac
     4561 tattggaata aagctttaac gaagtttcct gatttgcatt ttgaactcat agaagttaca
     4621 tctagtgtta attcagttgc tttatattac aaatcgatta tggataaaat ggctatcgaa
     4681 gtaatgtttt ttgatgataa aggattggta aataaaatga tagcacatta tactaattaa
     4741 atgaaaatag taatcataaa ttacggagca ggaaatattc aaagcattat gtttgccatc
     4801 gaaagattgg gatacaaagc ggttttgagc aacaatcctg acgaaataaa agcagccgac
     4861 aaagtgattt ttccaggagt gggagaggcg agttatgcaa tgaaaatgct tcaggaaagc
     4921 ggattggatg ttttaattcc tacgctgaaa caaccggttt ttggaatttg tctcggaatg
     4981 cagttgatgt gtaatt
//
LOCUS       DAOSNN010000203         4964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_17073_length_4964_cov_5.727032, whole genome shotgun sequence.
ACCESSION   DAOSNN010000203 DAOSNN010000000
VERSION     DAOSNN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17073_length_4964_cov_5.727032"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLQ70_09860"
     CDS             <1..978
                     /locus_tag="PLQ70_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-hairpin glycosidase"
                     /protein_id="HQE34959.1"
                     /translation="QYIPTRSPGFVNSNAKIWGQKTSDNRYATVYNPSEYRWPLAIST
                     SDDGLNYKDLLLVHGEISPMRYGGNYKSAGPQYVRGISETDGTPPDGKLWVSYSVNKE
                     DIWVASVPVPVTSEVKENANDVFNNLPNGEELKLWNTYDLAWASAKIEKKTDGKKSLT
                     LRDQDAFDYSRAERVIPFAQKMEATFIVTPEQNNHGLLQIEFQNKQGLPAIRLVFDSD
                     GELKAKNGARFGGITKYEAGKEYTVTVKLDVTSRSYTVKVNEGKETNKIFYAPVDGIS
                     RIMFRTGNQRYTPNADTEPDTPDFVDLPDTGKLIPEAVFNIKSLITKKL"
     gene            1178..3835
                     /locus_tag="PLQ70_09865"
     CDS             1178..3835
                     /locus_tag="PLQ70_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HQE34960.1"
                     /translation="MAENPLAVSQNQPRLSWQLVSGASNVLQTAYQIMVASSEKKLHN
                     DEADVWDSGKVVSNKNLQLSYDGKSLKSETKYFWKVKVWNQTSKASHWSKPAFFRTAP
                     SESDLNPIWIGAITKADSHLPEGRNYHTATFNKEKKASLINASDSLSRRSIMLRKPFS
                     VNKEIKEAVVYISGLGHYELVINGEKIGNSEFAPLWTDYDKTVNYNTYELTADELEKG
                     ENVIGVLLGNGMYNTLAERYSKFYVSFGPPTLFFKMKVIYKDGSEEIIKSDETWKYSK
                     SPITYNSIFGGEDYNANLEQKGWSNKGFNDANWKKIVVQEAPKGVLRAQTTTPIIVQK
                     QYEVKEAKEQKPNFYVFNMGQNLSGFTTIKVKGKKGQTVRVWVGESLNEDGTVGQGKT
                     GKPYYYDYTLKGDGVEEWQPKFSYYGFQYVQIENINYKEDKNKNLPTLVDLKSNFIYN
                     SAGEAGTFECSNDIFNKTHLLINNAIKSNFQSVFTDCPQREKLGWIEEIHLNGPGLMF
                     NYNLESYIPATMQNISDSQRDNGLIPTIVPEYVVFGGDFTDSPEWGVTGVILPWMYYE
                     QYGDASLIEKYYPVMKKYVDYLGTKATNHIVSHGLGDWYDYGTHAAGYSKNSPIALSA
                     TSHYFYGASLVAKAAKLLNKTDDIAKYETLTSDIKKAFNDKFFNAETKQYGTNSQFSN
                     AVPIFMDIVEPQYKAAVMKNLLNDIKAKGDRLTTGDVGNRYLFQALARNGENETMYKM
                     NNHYDAPGYGFQIKFGLTTLTEQWDPRKGNSWNHFMMGQIEEWFYQSLAGIVPDAENP
                     GFKHFFIQPEIVGDMTFVKATYKSIYGNIASAWEKKDGKLILKIEIPANTSATVKLPV
                     SKNSEIKVNGKPTKDLKLGSGKYTIECKI"
     gene            3986..>4964
                     /locus_tag="PLQ70_09870"
     CDS             3986..>4964
                     /locus_tag="PLQ70_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HQE34961.1"
                     /translation="MKNLKNITFTLLVSIFTLTLANAQCTSDTNFRKPVTETIKNIET
                     IFHITVIDDRGLLKGKELDYADWRIEQGNLEVSFANVLVPFELTYFKQPDGSYQIRKF
                     ENHKVSVDKGKERLEFLTNLYSNVSDWEARKKEIKACMITSFGLDKAPPMPKSKPILT
                     PKRIYKDYSVENIALEILPGVYTTGSIYKPYPLNKKSAIILTPDGHFGDGRYRKDEQY
                     RCAIMAKMGAIVVSYDLFAWGESLLQFPEETHRNSIASTVQVLSGIRLLDYLSTIKNA
                     DVTRVGVTGGSVGGSHTMFLAALDDRIKVSAPVVMVSSHFSGGCPCESGR"
BASE COUNT         1674 a          898 c         1065 g         1327 t
ORIGIN      
        1 caatacattc cgacaagatc acccggtttt gtcaacagca atgccaaaat ctggggacaa
       61 aaaacttctg ataatcgcta tgcaacggtt tataatccat cggaataccg ttggccattg
      121 gcaatttcaa ccagtgatga cggattgaat tacaaagatt tattgttggt tcacggcgaa
      181 atcagtccga tgcgttacgg tggaaactac aaatcggcag ggcctcaata cgttcgcgga
      241 atttccgaaa ctgatggaac tccgccggac ggcaaacttt gggtgagtta cagtgtgaac
      301 aaagaagata tttgggtcgc ttcggtaccg gttccagtga caagtgaagt gaaagaaaat
      361 gccaatgatg ttttcaataa tttgccaaat ggtgaagaat taaaattatg gaatacctat
      421 gatttggctt gggcttccgc caaaatcgaa aagaaaaccg atggcaaaaa aagcctaact
      481 ttgagagacc aagatgcttt tgattattcc cgtgccgaac gcgtgattcc gtttgcacaa
      541 aaaatggaag cgacttttat cgtaacgcca gagcaaaata atcacggctt gttgcaaatc
      601 gaatttcaaa ataaacaagg acttcccgcc attcgattgg tgttcgattc ggatggagaa
      661 ttaaaagcta aaaatggtgc ccgttttggt ggaatcacga aatatgaggc aggaaaggaa
      721 tacaccgtta ccgtaaaatt agacgttact tcccgttcct acaccgtaaa agtgaatgaa
      781 ggtaaagaaa cgaacaaaat attctatgct cccgttgatg gtatttcaag aataatgttt
      841 cgcaccggca atcagcgtta tacaccaaat gccgatacag aaccagatac accggatttt
      901 gttgatttac cagatacagg aaaattgatt ccggaggcag tatttaacat taaatcattg
      961 attacgaaaa agttataaaa atatatatca ccacaaatta cacaaattcc cgcaaatctt
     1021 ttagctgaaa taaaaagaat aatttgtgaa aatttgtgca atttgtggta aaaaaaaaga
     1081 aagcgtgaag tttttaaaaa acatagtaat ccttttttgt cttctgatat cgttcatttc
     1141 taatggacag atttccgttg cccatttaag atgtgaaatg gctgaaaatc cattagccgt
     1201 tagtcaaaac cagccaagat taagttggca attggtttcg ggagcgtcga atgtcttgca
     1261 aacagcttat caaataatgg tagcttcttc cgagaaaaaa ttacacaatg acgaagctga
     1321 tgtttgggat agcggaaaag tggtttccaa taaaaatctg caactatctt atgacggaaa
     1381 atctttaaaa agtgaaacta aatacttctg gaaagttaaa gtttggaatc aaaccagcaa
     1441 agcttcccat tggagcaagc cagccttttt caggacagca ccttcagaat ccgacttgaa
     1501 tccgatatgg attggtgcca ttaccaaagc cgacagtcat ttgccggaag gccgaaatta
     1561 ccacacggcc actttcaaca aagagaaaaa agcctcatta atcaacgctt ccgattcgtt
     1621 gtcccgcaga agtatcatgc ttcgcaaacc attttcggtt aacaaagaaa ttaaggaagc
     1681 cgtagtctat atttctggtt tggggcatta tgagttggtt attaacggcg agaaaatcgg
     1741 caacagcgag tttgcgcctt tatggacaga ttacgacaaa accgtcaatt acaatacgta
     1801 cgaattaacc gctgatgaac ttgaaaaagg tgaaaatgta atcggagttt tgttgggcaa
     1861 cggaatgtac aacacgcttg ccgaacgcta ttcgaaattc tatgtgagtt ttggtccgcc
     1921 gactttattt ttcaaaatga aagtgattta taaagacggt tcggaggaaa ttataaaatc
     1981 ggatgaaact tggaaataca gcaaaagccc gatcacctat aacagtattt ttggagggga
     2041 agattacaat gccaatttgg aacaaaaagg ttggagcaac aaaggattca acgatgccaa
     2101 ttggaaaaaa atagttgttc aggaagcacc aaaaggtgtt ttgagagcac agaccacaac
     2161 gccaataatc gttcaaaaac aatacgaagt taaggaagcc aaagagcaga aacctaattt
     2221 ttatgttttt aatatggggc aaaatctttc gggattcaca accataaaag tcaaaggtaa
     2281 aaaaggacaa accgttcgcg tgtgggtggg cgagagcctg aatgaagacg gaactgttgg
     2341 gcaggggaaa accggaaaac cgtattatta cgactatact ttgaaaggtg acggagttga
     2401 agaatggcag ccgaaattca gttattatgg ttttcagtat gttcagatag agaacatcaa
     2461 ttataaagaa gacaaaaaca aaaatcttcc aacattggtt gatttgaaat cgaattttat
     2521 ttacaattcg gctggagaag caggaacttt tgaatgttcg aatgatattt tcaacaaaac
     2581 tcacttactg attaacaatg ccatcaaaag taattttcag agtgttttta ccgattgtcc
     2641 gcagcgcgaa aaactgggtt ggatagaaga aattcacttg aatggtccag gcttgatgtt
     2701 caattataac ctcgaaagtt atattccggc tacgatgcaa aacatctcgg attcacaaag
     2761 agataatggt ctgattccaa cgattgttcc cgaatatgta gtttttggag gcgattttac
     2821 cgattctccg gaatggggtg taaccggagt gattctgcct tggatgtact atgaacagta
     2881 tggcgatgct tcgttaatcg aaaaatatta tcccgtaatg aaaaagtacg tggattatct
     2941 aggaaccaaa gcaaccaatc atatcgtttc gcacggattg ggcgattggt atgattacgg
     3001 aacacacgca gcaggatatt cgaaaaacag tccgattgca ctttctgcaa cttcacatta
     3061 tttttatgga gccagtttgg tggcgaaagc cgctaagttg ttgaacaaaa cggatgatat
     3121 tgctaaatac gaaaccttga cttcggatat taaaaaggct tttaacgaca agttttttaa
     3181 tgctgaaacc aaacaatacg gaaccaacag ccaatttagc aatgccgttc cgatttttat
     3241 ggatatcgtg gaaccgcaat acaaagctgc ggtgatgaaa aatttattga acgacatcaa
     3301 agccaaaggc gaccgattaa caacagggga tgtcgggaat cgctatttgt ttcaggcttt
     3361 ggcacgcaat ggcgaaaacg aaacgatgta caaaatgaac aaccattatg atgcgccggg
     3421 ttatggtttt cagataaaat ttggtttgac cactttgacg gagcaatggg atcctaggaa
     3481 aggaaattcg tggaatcatt ttatgatggg acaaattgaa gaatggtttt accaaagcct
     3541 tgccggaatt gtgcctgatg ctgaaaatcc tggtttcaaa catttcttca ttcagccgga
     3601 aatcgtgggc gatatgactt ttgtcaaagc gacttacaaa tccatttacg gaaatattgc
     3661 ttcggcttgg gaaaagaagg acggaaaatt aatcctgaaa atagagattc ctgccaatac
     3721 atcggcgaca gtaaaattgc cagtttcgaa aaattcagag ataaaagtca atggaaaacc
     3781 gactaaagat ttgaaattag gttcaggaaa atatacaata gaatgcaaaa tataacgata
     3841 aaatggaaca cggatgacac agattcgctg aagcgaagac gcagattatc acggatttta
     3901 tataaaattt aaaaaaatcc gtttttatcc gtgtttttac gaagtaaatc cgtgtcatcc
     3961 gcgtgccaat ctcaacaata ttaaaatgaa aaatttaaaa aacataactt ttactttact
     4021 agtttcaatc tttacattga cgcttgcaaa tgcgcaatgc acatcggata ctaatttcag
     4081 gaaaccagtt acggaaacga taaaaaatat tgaaaccatt tttcatatca cggttattga
     4141 cgaccgaggt ttattgaaag gaaaagaatt ggattatgcc gattggcgca ttgaacaagg
     4201 caatttggaa gtttcttttg ccaatgtttt ggttcctttt gagttgactt atttcaaaca
     4261 accggacgga agctatcaaa ttcgaaaatt cgaaaaccat aaagtttcgg ttgataaagg
     4321 gaaagaaagg ttggaatttc taaccaattt gtattcgaat gtttccgatt gggaagcacg
     4381 caaaaaagaa ataaaagcct gtatgatcac gtcatttggt ttggacaaag ctccgccaat
     4441 gcccaaatca aaacccattt taaccccaaa aagaatctac aaagattaca gcgttgagaa
     4501 catcgcattg gaaattttgc caggcgttta caccacggga tcgatttaca aaccctatcc
     4561 gttaaacaaa aaatcggcaa ttatcttgac gcccgacgga catttcggcg acggaagata
     4621 cagaaaagac gagcagtacc gttgcgccat aatggcgaaa atgggagcaa tcgtagtgag
     4681 ttacgattta tttgcctggg gcgaatcctt gttgcaattc cccgaagaaa cgcaccgaaa
     4741 cagcattgca tcaaccgttc aggtattgag cggaatacga ttgttggatt atttgtcgac
     4801 cataaaaaat gccgatgtta caagagttgg cgttaccgga ggttctgttg gcggttcgca
     4861 cacgatgttt ttggcagctt tggacgacag gattaaggtt tctgctcctg ttgttatggt
     4921 gtcttcgcat ttttcgggag gatgtccgtg cgagagtggt cgcg
//
LOCUS       DAOSNN010000204         4955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_17123_length_4955_cov_6.394082, whole genome shotgun sequence.
ACCESSION   DAOSNN010000204 DAOSNN010000000
VERSION     DAOSNN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17123_length_4955_cov_6.394082"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PLQ70_09875"
     CDS             <1..977
                     /locus_tag="PLQ70_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQE34962.1"
                     /translation="GADGVRVGLLLSASAGNDIMFDEELCNQGKGFTNKIWNAFKLIK
                     GWEVSDSIPQPESSKVAIEWYEAKLQQTLLEIEDNFEKYRISDALMGIYKLVWDDFCS
                     WFLEMIKPGYQQPIDSVTLAKAIEMLENNMKLLHPFMPFLTEEIWQLLDERTPEEALI
                     VSTWPEIKPFNAQLITDFENTIEVISGIRTIRKDKNIPFKDTIELKVVNYDKASTYFD
                     AVVTKLGNITSLEYVSDKVDGALSFRVKSNEYFIPITGNIDVEAEIAKLTEELNYIKG
                     FLKSVQVKLSNEKFVANAKPEIIENERKKEADALSKIATIEQSLAGLK"
     gene            complement(1211..3457)
                     /locus_tag="PLQ70_09880"
     CDS             complement(1211..3457)
                     /locus_tag="PLQ70_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HQE34963.1"
                     /translation="MKTILLPVILLLGALNGFAQETASSTEKNNTVEGFYSDSDTIKG
                     KKREVLKEVIVTTNKQPKPVTALRSGLKPMDTPQSVQVIGSEIIEQQQAIRLSEVLKN
                     ANGVYVGSARGGAQESFFSRGYDMSANNMFKNGFRYNGGSIPDVSGLEKVEFLKGGSA
                     LLYGNVAPGGILNLVTKTPSFKSGGEISMQAGSYSYYKPSIDFYGPLNKFIAYRINGS
                     YENSESFRDVVKNERLYVNPSLLFNVSDKTQITVQGDYLNADWTPDFGTGIIGKELVD
                     VPRNNFYGALWSTGKTKSASASALVNHDFNKNWKLNFNSSFQTYNRTSKSTAQLSTVT
                     ANGDWTRPLVENKNLEQIVGNQLSLQGTFHTGSIKHQLFTGVDFENSFATAYTFAFTP
                     ANYDTINLYNFDPSTQRNDIPNSKNTRIVKTDTNRFGVYAQDLVSFTEKIKLLIGLRW
                     SWQEATVNTYDLTKTPTVLTITPARKDEALSPKVGLVYQPTKDMSLFASYSNSFTPNT
                     GTTVDLQPIEASIIDQYEAGVKKDFWKGILSTNVTVYQITNSNLAQTAELKADGTPNT
                     DTSIKTLGGGTKSKGIEVDITARPLKGMNINGGYSYNDMRYTNTSGTSGSFVEGDRVV
                     RTPRNTANLSFFYTLPSGVLKGLSFGAIGNYIGDRLGGWNDDYVWTEKKPATNPKTYT
                     VTIRDRDIPLEGYTTIDASVGYTWKKISVLCKLSNITNELNYTVHENYSVNPIAPRQV
                     LTSIKYKF"
     gene            complement(3782..4765)
                     /locus_tag="PLQ70_09885"
     CDS             complement(3782..4765)
                     /locus_tag="PLQ70_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019974647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQE34964.1"
                     /translation="MTIKHFFLFLILCGFSSHSQVLRKRTTLLMGGRFDISIVAKDSL
                     LSEQNIDIVIAEISRIENLISDWKPTSQVSEVNQNAGIRPVKVDREVFELTQRAIRFS
                     EITNGGFDISFAAMDKIWKFDGSMTEMPSAEAIKKSVEKVGYKNIVLDSLNSTIFLKL
                     KGMKIGFGALGEGYATNKCRSMMLTKGIEAGIINGSGDMSTWGKQPNGKAWNIGITNP
                     FDTDKLVAVVPINNGAVTTSGSYEKFVVFDGKRYSHIINPATGYPATGLCSVTVFGPN
                     AETANGLSTSMMVLGKTEGLLLLEKFPDYSCLMITDTGKVVKSKNFKKKRK"
BASE COUNT         1478 a          914 c          984 g         1579 t
ORIGIN      
        1 ttggtgcaga tggtgttcgt gtagggttgc ttttgagcgc ttcagcagga aacgatatta
       61 tgttcgacga agagctttgt aatcaaggaa aaggttttac caataaaatt tggaatgctt
      121 tcaaattgat aaaaggttgg gaagtttcgg attcgattcc gcaaccagaa tcttcaaaag
      181 tggcgattga atggtacgaa gccaaattac aacaaacgct tttggaaatc gaagataatt
      241 tcgagaaata tagaatttcc gatgccttga tgggaatcta taaattggtt tgggacgatt
      301 tctgttcttg gtttcttgaa atgataaaac cgggatacca acaaccaatt gacagcgtaa
      361 ctttggcgaa agccatagaa atgctcgaaa acaatatgaa attgttgcat ccatttatgc
      421 cgttcttgac agaggaaatt tggcaattat tggacgaaag aacgccagaa gaagcgttaa
      481 tcgtttcgac ttggccagaa atcaagcctt tcaatgcgca attaatcacc gatttcgaaa
      541 acacgattga agtgatttcg ggaatcagaa cgattcgtaa agacaagaat attccgttca
      601 aggacacgat tgaattgaaa gtggtgaatt acgataaagc ttcgacttat tttgatgcgg
      661 tggtaaccaa attggggaac atcacatcgt tggaatatgt ttcggataaa gtggatggcg
      721 ctttgtcttt ccgtgtcaaa tcgaacgaat atttcatccc gattacagga aacattgatg
      781 tggaagccga aatcgccaaa ttgaccgaag agttgaatta catcaaaggt ttcttgaaat
      841 cggtgcaagt gaagctgtcc aacgaaaagt ttgtggcaaa cgccaaaccc gaaatcatcg
      901 aaaacgaacg caaaaaagaa gccgacgcgc tttccaaaat tgccacaatc gagcaaagtt
      961 tggcaggatt gaaataattc ggttccgttt ttaaaataga aatcccaatg ctgaaatttt
     1021 tcaggattgg gatttttttt gaattaaaaa atttgagatt ctatattttt tgcaaaaatt
     1081 acaagtgatg atttgaaaat taactactac aatattagaa ctcgggtaaa aaaccaaaac
     1141 cttttctact tatcaagatt gaaagcggta gaaaaaggta taggcaataa aacaaactga
     1201 aaatctttat ttagaattta tattttatgc tagttagcac ttggcgtggc gcaatcgggt
     1261 ttacgctata attttcgtga acggtgtaat tcaactcgtt ggtaatgttg gataatttgc
     1321 aaagaactga gatttttttc caagtataac ccacagaagc gtcgatggtg gtgtatcctt
     1381 ctagcggaat atctcggtct ctaatagtta cagtataggt ttttgggttt gtcgcaggtt
     1441 ttttttctgt ccacacataa tcatcattcc aacctcctaa acggtcacca atatagtttc
     1501 cgatggctcc aaaggaaaga cctttcaaga ctcccgaagg caaggtgtag aaaaagctta
     1561 gattggcagt atttcgggga gttcttacca cgcggtcgcc ttcaacaaaa ctgccacttg
     1621 ttcctgaagt attcgtataa cgcatatcgt tgtagctgta tccgccatta atattcatcc
     1681 ctttcagtgg tcttgcggtg atgtcaactt caattccttt gctttttgtt cctccgccca
     1741 atgttttgat actcgtatcg gtattgggtg ttccgtcggc ttttagttcg gcagtttggg
     1801 ccaaattact gttggtgatt tggtaaacgg tgacattggt actcaaaatt cctttccaaa
     1861 aatctttttt gactcctgct tcatattggt caatgattga ggcttcaata ggttgcaaat
     1921 cgactgttgt tcctgtattt ggcgtgaacg agttagagta gctcgcaaat aatgacatat
     1981 cttttgttgg ctgatagact aatcccactt ttggcgaaag tgcttcgtct ttgcgggcag
     2041 gagtgatggt aagaacagtt ggcgtttttg ttaaatcata ggtgttaaca gtggcttctt
     2101 gccaagacca acgcaatcca attagcaatt taattttttc agtaaatgaa actaaatctt
     2161 gagcataaac tccaaaacgg ttggtatctg tttttacaat tctcgtgttt tttgagtttg
     2221 gaatatcatt tctttgagtt gaaggatcaa aattatagag attgatggtg tcataattag
     2281 ccggagtaaa cgcaaaagtg taagccgttg caaatgaatt ttcaaagtca actcctgtaa
     2341 ataattggtg tttgatgctt cccgtgtgga atgttccttg caagctcaat tgatttccta
     2401 ctatttgttc taggttttta ttttcaacca aaggtctggt ccaatcgcca ttggcggtaa
     2461 cagttgataa ttgtgctgtc gattttgaag ttctgttgta tgtttgaaac gaactgttga
     2521 aattcaattt ccagtttttg ttgaaatcgt ggtttaccaa tgcagatgca cttgccgatt
     2581 tggtttttcc agttgaccaa agtgctccat agaagttgtt tcgaggcaca tcgactaatt
     2641 cttttccgat aattcccgtt ccaaaatcgg gtgtccaatc agcattcaaa taatctcctt
     2701 gaacagtgat ttgggtttta tcgcttacat taaaaagtaa agaaggattt acatacaaac
     2761 gctcgttttt cacgacatcc ctgaaacttt ctgaattttc ataagaacca ttaattctat
     2821 aagcaataaa tttattcaaa ggaccgtaaa aatctatcga aggtttgtaa taggaataac
     2881 ttcctgcttg catcgaaatt tcgccaccgc ttttgaaaga aggtgttttg gttaccaaat
     2941 tcaaaattcc gcctggagct acatttccat ataaaagtgc cgaacctcct tttaggaatt
     3001 ccactttttc aagacctgaa acatcaggaa tagaacctcc attataacga aaaccgtttt
     3061 taaacatatt attggcactc atatcatagc ctctagagaa aaaagattct tgtgctccac
     3121 cacgggcgga acccacataa acaccattgg catttttcaa tacttcactc aatcggatgg
     3181 cttgttgttg ttcgatgatt tccgaaccaa ttacctgtac actttgtggt gtgtccattg
     3241 gttttaatcc agaacgtagg gcagtaacag gtttgggttg tttgtttgtt gttacaatta
     3301 cttctttaag aacttctctt ttttttcctt taatggtgtc agaatcagaa taaaatcctt
     3361 caacagtatt gtttttttca gttgaacttg ccgtttcttg ggcaaatccg tttagtgcac
     3421 ctaataagag tataacaggg agtaaaatag ttttcattta tatttattta gactgtttaa
     3481 aaataacagt acaaatatat aaaccatttt tgattacaca aatttattta gactaaatct
     3541 taatagcgat ttattaactt ttttttaagg attgattatt agtgttttat gatgtaaaaa
     3601 acagacaaaa aaataggctt tttgggcaaa gtaaatagaa agatgagtcc gtttcaatga
     3661 gtaattggcg aagtagataa gaaaaaaagg gattatattc aagtgatgaa agcaaatttg
     3721 aaaagcctac gttttcattt agctaatcat tatgagttaa ggactaatca ctaaaatcca
     3781 atcactttct tttctttttg aagtttttcg atttcacgac ttttcctgta tcggtaatca
     3841 tcaggcaact ataatccggg aatttttcga gcaaaagaag tccctctgtt ttacctaaaa
     3901 ccatcatcga agtgctcaaa ccattggccg tttcggcatt gggtccaaaa accgttacac
     3961 tgcacaaacc cgttgccgga taacccgttg ccggattgat gatgtgcgaa tagcgtttgc
     4021 catcaaaaac gacgaatttt tcatagctgc cggaagtggt cactgcacca ttgtttatcg
     4081 gaacaacggc aacgagtttg tcggtgtcaa aaggattcgt gatgccaatg ttccaggctt
     4141 tgccgttggg ttgttttccc caagtgctca tatcgcccga accgtttatg attccggctt
     4201 cgatgccttt agtcagcatc attgatcggc atttgttggt ggcatagcct tcgcccaaag
     4261 cgccaaaacc aattttcatt cccttcaatt tcaagaaaat ggtcgaattt agactgtcca
     4321 aaacaatgtt tttatagccc actttttcca ccgatttttt gatggcttcc gccgaaggca
     4381 tttcggtcat agagccgtca aacttccaga ttttgtccat tgccgcaaaa ctgatgtcga
     4441 agccaccatt ggttatttca gaaaaacgga tggctctttg ggtcaattca aaaacttctc
     4501 gatccacttt cacgggacga atgcccgcat tttggttcac ttccgaaact tgtgacgtgg
     4561 gtttccaatc cgaaatcaaa ttttcgattc ggctgatttc ggcaatgaca atgtcaatat
     4621 tctgttcgga caacaaagaa tccttggcca cgatgctgat gtcaaaacgg cctcccatca
     4681 acaaagtggt tctttttcgc aagacttggc tgtggctcga aaagccgcaa agtattaaaa
     4741 aaagaaagaa gtgtttgatg gtcatattaa aatttatgta ggtcgaaatg acgaaaaaaa
     4801 ataataaaag gagaaaccaa cttattgtat aaaaaaccaa ccgtggcatc ctgcaactgc
     4861 tcccgcaatt gttatcaagc tgatgatgag caaaacccaa tgtaatcggt gcattatcct
     4921 gaatgttttt tcggggtttt cggtggcata ttttt
//
LOCUS       DAOSNN010000205         4951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_17145_length_4951_cov_4.485703, whole genome shotgun sequence.
ACCESSION   DAOSNN010000205 DAOSNN010000000
VERSION     DAOSNN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17145_length_4951_cov_4.485703"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLQ70_09890"
     CDS             <1..566
                     /locus_tag="PLQ70_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34965.1"
                     /translation="EGRQGVASFFPYKVGDKSLGFYSGAFPFQSWADYPKKSGKGWFV
                     GLAESNSLEGPWSRIKDGSNPIKSMHPFFVENPIVSQLPNGLYIAVFDGGPDGWGHHL
                     PNMMAYSLSADGKKWSEAHYLPIETKVDKWWDIMRTPLCLIPEGNDVYTIVYNAIDLK
                     RRFHPTGMVKLKLNKDVMESRLKELEK"
     gene            680..1810
                     /locus_tag="PLQ70_09895"
     CDS             680..1810
                     /locus_tag="PLQ70_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylase"
                     /protein_id="HQE34966.1"
                     /translation="MKSTIKYLAIGMVMLFIASCATKFKKREITETVMQEIYNEIKTP
                     YKYGLVMVPTDNSYKMDCPSVFRKDGKWFMTYLIYDGRGYETWLAESNDLLNWKHLGK
                     VMSFSKDETHWDVNQKAGYIALQDYTWGGSYEWKKYDDKYWMSYFGGNTTGYEAGVLS
                     IGMAYTKEAPTKPHEFERLPNPVLTPKDTDVSFWDNSTMYKNSVIEDKEKLTGSKFIM
                     YYNARGDSLNPAKGAERIGMAVSNDMKTWKRYGKKPLLNHHRGITGDAYIQRVNDTWV
                     MFYFGAFWRDWNQGAFNRFAVSNDLVNWKEWKGDDLIKSSEPYDDKFAHKSFVVKHNG
                     VVYHFYCAVNKADQRGIAVATSKDLGKSEMNFVAPPEKKEKK"
     gene            1930..>4951
                     /locus_tag="PLQ70_09900"
     CDS             1930..>4951
                     /locus_tag="PLQ70_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQE34967.1"
                     /translation="MFSNSARLGISVLILNTFVLILPFLGNAQSVTGEPAGIPELHKR
                     YEFAPWEDPTVTSINRQPSRATGYSYASVEDALKGDRTKSRIQMLNGDWNFKYAVNLK
                     EASKDFYKTTVSGWDKIEVPSNWEMKGYDNPIYKSAVYPFRPINPPYIPKDYNGVGSY
                     QRSFTVPANWKDMTVTLHFGGVSSGFQVWLNGEFLGYGEDSCLPSEFDITPYLKEGEN
                     VVSVQVIRWADGSYLEDQDHWRVSGIQREVFIMAEPKLRIQDFFYQTKLDKEYKDAVF
                     KLRPKVENLTGAKIKDYTFQAQLYDADNKPLFKEPLQKPVIDMINESYPRLDNVRFGM
                     FQETIKNPKKWSSEEPNLYTLVMSIKDPNGNITESKSCKVGFRSIEFSKENGKMLING
                     KETYVYGINRHDHNPVRGKALTREDIKQDITTIKKFNFNFIRTSHYPNDPYFYELCDQ
                     YGIMVMDEANQETHGIGGKLSNDPKWTNAYLERMTRMVYRDKNHPSVVFWSLGNEGGK
                     GPNHSAMAGWVHDFDITRPVHYEPAQGNAKLDGYIDPLDPRYPKTIDHAYRFENPQDD
                     SYVDMVSRFYPGVFTPKFLVDQKKDTRPIIFVEYSHAMGNSVGNLKELWDEFRSLPRV
                     IGGCIWEFKDQGLVKFDSRSGQYYFAHGGDFGEKYHDGNFNTKGLVDSNGKPKGSMYE
                     NKWIYQPATSTLKDGNQLEIKNRQAVKSLADYIPVLKILENGNVIKTQVLKPFNLEAG
                     QSTVLNVNPYLPKMKSDAEYYLNIEFQLSKDEPWAPKGYSVAEDQFLLKKKEAVSPIV
                     KKENLNVSESDSGFKIKGKSFDINISKANGALSSYVFNGVEQIFSPLLPNFVRPLTDN
                     DKRGWKSNKVLKQWYKAKPKLTNIKMDKSNVEIKITSDYEIIKDSANVSVVYNIKPDG
                     TIKVDYSLKASNKLPNIPKIGMQMGVQRKFDQISWYGKGELENYSDRSFGSFVGKYSL
                     PINDFIEHYPKPQENGNRCDVRWMALTTPQKNKGF"
BASE COUNT         1674 a          850 c         1091 g         1336 t
ORIGIN      
        1 gggaagggcg tcaaggcgtg gcttcttttt ttccgtataa agtgggcgat aaatcccttg
       61 gattttatag cggagcattt ccgtttcaat cttgggctga ttatcccaag aaatctggaa
      121 aaggttggtt tgttggtttg gctgaatcga atagtttgga aggtccttgg tcacgaataa
      181 aagacggcag caatccaatc aagtcgatgc atcctttttt tgtggagaat ccaatagtga
      241 gccaattgcc aaacggtctg tatatagccg tttttgatgg tggcccagac ggttgggggc
      301 atcatttacc caatatgatg gcgtattcgc tttctgctga tgggaaaaaa tggagtgagg
      361 cgcattattt gccaatagaa acaaaagtcg ataaatggtg ggatattatg agaacgccat
      421 tgtgtttgat tcccgaagga aatgacgttt atacaatcgt atataacgcc atcgatctga
      481 aaagaagatt tcatccgaca ggaatggtaa aactaaaatt gaacaaagac gtaatggaat
      541 cccgattgaa ggaattagaa aaataggcca cggataacac ggattaagcg gatctacact
      601 gattgtttaa ttttaaaatc tgagaaaatc agtttaatcc gagtcatccg cgtcccattg
      661 aagcaaataa cattagaaaa tgaaaagtac aattaaatat ctcgcaattg gaatggtaat
      721 gttattcata gccagttgcg ccacaaaatt caaaaaaaga gaaatcaccg aaaccgtaat
      781 gcaggagatt tacaacgaaa tcaaaacgcc ttacaaatat ggtttagtga tggtgccaac
      841 ggacaactcc tacaaaatgg attgcccgtc ggtttttaga aaagacggaa aatggtttat
      901 gacgtatcta atttatgatg gacgtggtta cgaaacctgg ttagccgaaa gcaacgattt
      961 gctaaattgg aaacatttgg gaaaagtaat gtcgttttcg aaggatgaaa cccattggga
     1021 cgtgaaccaa aaagcaggct atatcgcatt acaggattat acttggggcg gttcgtacga
     1081 gtggaaaaag tatgacgata aatattggat gagttatttc ggaggaaata ccacgggtta
     1141 tgaagcgggc gttttgtcta tcgggatggc atatacgaaa gaagcgccaa ccaaaccaca
     1201 cgaatttgaa cgtttaccaa atccggtttt aacgccaaaa gatactgatg tgagtttttg
     1261 ggacaacagt acgatgtata aaaattcggt gattgaagat aaggaaaaat tgacaggcag
     1321 taaatttatt atgtattaca atgcccgtgg cgatagtttg aatcctgcaa aaggtgctga
     1381 acgtattgga atggcagttt ctaatgatat gaaaacctgg aaacgttacg gcaaaaagcc
     1441 tttacttaat catcatagag gaattacggg ggatgcctat attcaacgtg ttaacgatac
     1501 ttgggtgatg ttttattttg gtgctttttg gagagattgg aatcaaggtg catttaaccg
     1561 ttttgccgtt tcgaatgatt tagtaaactg gaaagaatgg aaaggtgatg atttaatcaa
     1621 atcatcggag ccttatgatg ataagtttgc acacaaatca tttgtggtaa aacataacgg
     1681 tgtggtgtat catttttatt gcgccgtgaa caaagcagat caacgcggaa tagccgtggc
     1741 aacttccaaa gatttaggta aaagtgaaat gaattttgtg gcaccaccag agaaaaaaga
     1801 gaagaaataa aagtattaag tacgaagaat taagtattaa gatttaaata gtaagttttg
     1861 aaattaaata aaagaaatat aatccatttt gttcagcatt tttttgagga aaaatgttcg
     1921 aaaccattaa tgttttcaaa ttcagctcgt ctgggtatat cggtcttaat acttaatact
     1981 tttgtcttaa tacttccctt tttagggaat gcccaatcag taaccggcga acctgcggga
     2041 attcccgaac tgcacaaaag atacgaattt gccccttggg aagacccaac ggttacaagc
     2101 atcaacaggc aaccttccag agcaacgggc tattcttatg ccagcgtgga agacgcatta
     2161 aaaggcgaca gaaccaagag ccgaatccaa atgctgaacg gcgattggaa tttcaaatat
     2221 gcggtaaact taaaagaagc gtcgaaagat ttttataaaa ccacggtatc aggctgggat
     2281 aaaatcgaag tgccttcgaa ctgggaaatg aaaggctatg ataatccaat atacaaaagt
     2341 gcggtttatc catttagacc cataaatccg ccttatattc ctaaagatta taacggagtc
     2401 ggttcctatc aacgcagttt taccgttcct gcaaattgga aagatatgac cgtgactttg
     2461 cattttggag gtgtaagttc cggctttcaa gtttggttaa acggggagtt tctgggttat
     2521 ggagaagaca gttgtctgcc atcagaattc gatatcacgc cttatttgaa agaaggtgaa
     2581 aatgtggttt cggttcaggt aattcgctgg gctgacggtt cgtatctgga agatcaagat
     2641 cactggcgcg tgagcggaat tcaacgtgaa gttttcatta tggcggaacc aaaactacgc
     2701 attcaggatt tcttttacca aacgaaattg gacaaggaat acaaagacgc cgtttttaaa
     2761 ttgcgtccaa aagtagaaaa cttgacaggt gcgaaaatca aagattacac gtttcaagca
     2821 caattatacg atgcagataa caagccgtta ttcaaggagc ctctccaaaa accggttatc
     2881 gatatgatca acgaaagtta tcctcgcttg gataatgtgc gttttggaat gtttcaggaa
     2941 accatcaaaa acccgaagaa atggagttct gaagagccca atctctatac tttggtgatg
     3001 tccatcaaag atcccaacgg caatataacc gaatcaaaat cctgtaaagt gggtttccgt
     3061 tccatcgaat tttcgaaaga aaatggcaaa atgctcatca acggaaaaga aacttacgtt
     3121 tacggaatca atcgccacga ccataatccg gttcgaggaa aagccttgac cagagaagat
     3181 attaaacaag atattaccac aatcaagaag tttaacttca attttattcg caccagccat
     3241 tacccaaacg atccttattt ttatgaatta tgcgatcaat acggtattat ggtaatggac
     3301 gaggccaatc aagaaaccca cggtattgga ggaaaattga gcaacgaccc caaatggacc
     3361 aacgcttatt tggagcgtat gacccgaatg gtgtatcgag acaaaaatca tccttcggtt
     3421 gttttctgga gtttgggtaa cgaaggtgga aaaggcccaa accattccgc aatggcgggt
     3481 tgggtgcacg attttgacat tactcgtccc gtgcattacg aaccggcaca agggaatgcg
     3541 aaattagatg gttacatcga tccgctcgat ccaagatatc ctaaaacgat cgatcacgct
     3601 taccgattcg aaaatccgca agatgattcg tatgttgata tggtcagccg tttttatccc
     3661 ggagttttca cacctaaatt tttggtagat caaaagaagg atacaagacc gattattttc
     3721 gttgaatact cacacgcaat ggggaattca gttgggaatt taaaagaatt atgggatgaa
     3781 ttccgttcat tgccaagagt tattggcggt tgtatctggg aattcaaaga tcagggattg
     3841 gtgaaattcg actccagatc aggtcaatat tattttgccc acggaggtga ttttggtgaa
     3901 aaataccacg acggaaattt caatactaaa ggtttggtag attctaatgg aaagccaaaa
     3961 ggctcgatgt atgaaaacaa atggatttac caaccggcga cttcaacttt gaaagatggc
     4021 aatcaattgg agattaaaaa tcgtcaggcg gtaaaatctt tggcagatta tattccggtt
     4081 ttgaaaatat tagaaaacgg aaatgtcatc aaaacacaag ttttaaaacc tttcaattta
     4141 gaagctgggc aatccacggt tttgaatgtg aatccgtatt tgccaaaaat gaaatcggat
     4201 gcggaatatt atttgaatat cgaatttcaa ctttcgaaag atgaaccttg ggctccgaaa
     4261 ggctattcgg ttgcagaaga tcaatttttg ttgaaaaaga aagaagctgt ttctccaata
     4321 gttaaaaaag aaaatctgaa cgtttccgaa tccgattcgg gattcaaaat caaaggaaaa
     4381 tcgttcgaca tcaatattag taaagcaaat ggtgcgttga gttcttatgt ttttaatgga
     4441 gtagaacaga ttttttctcc cttgttgcca aatttcgtca gaccacttac agacaatgac
     4501 aaaagaggtt ggaagtcgaa caaagttttg aaacaatggt acaaagccaa accaaaattg
     4561 acgaatatca aaatggataa gtcaaacgtg gagataaaaa tcaccagcga ttacgaaatt
     4621 ataaaagaca gtgccaatgt gagtgtggtt tacaacatca aaccagacgg aacgataaaa
     4681 gtggattaca gtttgaaagc atctaacaag cttccaaata ttccgaaaat tggaatgcaa
     4741 atgggtgtcc aaagaaaatt tgaccaaatt tcgtggtatg gaaaaggaga attggagaat
     4801 tacagcgaca gaagttttgg ttcattcgtt ggaaaatatt cactcccaat caatgatttt
     4861 atcgaacatt acccaaaacc gcaagaaaat ggaaaccgat gtgatgtgag atggatggca
     4921 cttacaactc cgcaaaaaaa caagggtttt g
//
LOCUS       DAOSNN010000206         4943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_17183_length_4943_cov_4.310966, whole genome shotgun sequence.
ACCESSION   DAOSNN010000206 DAOSNN010000000
VERSION     DAOSNN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17183_length_4943_cov_4.310966"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLQ70_09905"
     CDS             complement(<1..266)
                     /locus_tag="PLQ70_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQE34968.1"
                     /translation="MKKIKDKKEDNKTSSWTVCPECKGYSKKSRGITKKALLKYQKAF
                     DQFEKTNNEGTPPVLPKGHLHLCPKCDGSGLISSANFPIPDTEN"
     gene            complement(616..1656)
                     /locus_tag="PLQ70_09910"
     CDS             complement(616..1656)
                     /locus_tag="PLQ70_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQE34969.1"
                     /translation="MIQFIKIDEHSRVPKYKQIVDSIIFNISIGNLKIDQKIPSINRF
                     SEDFYISRDTVEKAYNILKEREIISSIKGKGYYITGTKLVSKLNILFLINKLSTYKMT
                     IYNSFVNSIGTNSNTDLQIYHCDESLFLNLLDKNKNNYDYYVIMPHFKTNDSRHVSFT
                     TEIIDAIKQIPKEKLIVLDNIKLALDNDIIEIYQDFENDIYNALIEGLPKINNYDKLI
                     LVYPEKAVYPYPRRILSGFKKFCGEHSISFEILNEVYDDMVIKKGDLFITIEESDLVN
                     LVKIIRDSKLTLGKDIGIISYNDTPLKELLGITVISTDFKVMGETASFMILNKEKGKV
                     KVPFNFIDRNSI"
     gene            2171..3220
                     /gene="rhaT"
                     /locus_tag="PLQ70_09915"
     CDS             2171..3220
                     /gene="rhaT"
                     /locus_tag="PLQ70_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HQE34970.1"
                     /translation="MQVLLGIIFHSIGGFSSGSFYMPYKKVRGWAWESYWIIGGFFSW
                     LIVPPLAAYLTVPGFVDIISNAAPTVKAVTYTMGLIWGIGGLTYGLGVRYLGMSLGNS
                     ITLGFCSAFGALVPSIYYNFYPVAGKTSFTDMLHSTGGQMVLLGVVVCLVGIAFSGKA
                     GMLKEKDFATGHEDKDKDFSLVKGLIIAIISGILSSFFNFGIEAGKPMAEEAVAQGFN
                     PLFQNNVTYIVILWGGLTINFLWCMYLNFKNKTFGDYTNSQTPITKNIMFSALAGTMW
                     FLQFFFYGMGESKLGNGASSWILHMATIILTANFWGFYLKEWTGVSKKTFNTFLIGIG
                     LILASIVIVGIGNSL"
     gene            3255..>4943
                     /locus_tag="PLQ70_09920"
     CDS             3255..>4943
                     /locus_tag="PLQ70_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HQE34971.1"
                     /translation="MSNINSNNMTFKHVSYLWDEAKAASMAGDEVALFIYRSNLLGAD
                     LRLTNYGGGNTSVKITDKDPLTGAASEVMWIKGSGGDIGTLTKSGCAALYLERLLNLE
                     NVYRGIEFEDEMVELFNHCIFDLASKAPSIDTPLHGFLPFKHIDHLHPDAAIAIAAAK
                     DGKKITEELFNGEIGWVGWQRPGFDLGLQMRACLQEAEAKGKKLKGVMLGSHGLFTWG
                     DTAYESYINTLEVIEKCAEYLESNYGKTRPVFGGQKLESLPEDARQLQAAKLAPILRG
                     FCSSERKMIGHYTDDSRVMEFINSNDLEKLAPLGTSCPDHFLRTKISPLVIELAPNED
                     LSDVAAIKAKLTPAFEAYREMYKEYHNTCKKSNSPAMRDPNPVVILYPGVGMFTFAKD
                     KTMARLAAEYYTNAVNVMKGAEAVSEYTSLPRQEAFDIEYWLLEEAKLQRMPKPKALS
                     GRIAVITGSAGGIGKAIAKKFAEEGACVVINDINEERLQGATADFVKAFGKDAVSSTL
                     LNVTDEKSTELALDAACLAFGGADIIVNNAGISISKSIAEHSLEEWDRLYDILVK"
BASE COUNT         1573 a          697 c          925 g         1748 t
ORIGIN      
        1 taattctctg tgtcaggtat agggaaatta gcggaagaaa tcaatccaga accatcgcat
       61 tttgggcata aatgcaaatg gcctttaggt aaaacaggag gtgttccttc gttgtttgtt
      121 ttttcaaatt gatcgaaggc tttttggtat ttgagtaacg cttttttggt tattccgcga
      181 ctttttttgc tgtatccctt gcattcagga caaacagtcc aacttgatgt tttgttgtct
      241 tcttttttat cttttatttt tttcacttga ttttgatttt ctataaaaga aaacttgctt
      301 atagtattga ttatggttgg gcaaagttaa ggatttctag caattagaat tgtagggttt
      361 aattttaatc gttttttcaa gctatagtca gaaattaatt tatagtatag gagggatgag
      421 catacttctt tttcacggat tttttagaaa cactactatt tgattcgaga ataagtctat
      481 agagttcacg aaaaaccact caagcgagtg tctttttatt ttatatcagg aatgacgtga
      541 atttaagtct gtatttttat aaaaaatagc ctcgtaacca tataattaca aggctttttt
      601 tttgtatttt ttggtttaaa ttgaatttcg gtctataaaa ttaaaaggaa cttttacttt
      661 tcctttttct ttgttgagaa tcataaacga agcggtttca cccataactt tgaagtcggt
      721 tgaaataaca gtaatgccta gtaattcttt tagaggagtg tcgttgtatg agatgatgcc
      781 aatgtctttg cctaaagtaa gtttggaatc tcgaatgatt ttgaccaaat taaccaagtc
      841 agattcttcg atggtgataa ataaatcacc ttttttgata accatatcgt catatacttc
      901 attgagaatt tcaaaactga ttgaatgttc tccgcaaaat ttcttaaaac cacttaaaat
      961 ccttctagga tagggataaa cagctttttc aggatatact aatataagtt tatcgtagtt
     1021 gtttatttta ggaagacctt cgataagtgc gttataaata tcgttttcaa aatcctgata
     1081 tatttctatt atatcgttat ctaaagctag ctttatattg tctaaaacta ttagtttttc
     1141 tttcggaatt tgttttatag catcgataat ttcagtggta aaactaacat gtcttgaatc
     1201 attagtttta aaatgtggca ttattacata gtaatcatag ttgtttttgt ttttgtccaa
     1261 taaattcaaa aacaaggatt cgtcacagtg gtatatttgt aaatcggtat tcgaatttgt
     1321 gccaatgcta tttacaaaag aattgtaaat agtcattttg taagtgctta atttatttat
     1381 taaaaataga atattaagct ttgaaactaa ttttgttcca gtaatataat acccttttcc
     1441 tttgatggat gaaattattt cacgttcttt taggatgttg tatgcttttt caacagtatc
     1501 tctagaaata taaaaatctt cactaaatct attgatcgaa ggtatttttt ggtcaatttt
     1561 tagatttcct atagagatat taaaaataat cgagtcaaca atttgcttgt attttggtac
     1621 tcgcgaatgt tcatctattt taataaattg aatcatattc atattaaaaa atattttata
     1681 atttgattat tttttttgca aaagtaaaga atatagacaa aactaactgt ttaatgtttg
     1741 atactattta ttttattgtg tgatgttttg gctgaaaatt ctaaatagaa taattagttt
     1801 cagtttttgt tttcgagatt ttagaaagaa tcaatattag ttaaaaatag attattcaaa
     1861 agtatgtctt tttaatcaaa aagccgaata ctttaacggt aaatttagaa actttaaagt
     1921 gtttgttttt aacattttac acattgtttg cattaaaaat ggaaatttat cagagcataa
     1981 ttcattaaaa attaagatat aaatttaata ttatgtggtt ttgaaagatt aaaattactc
     2041 atcaaaaaaa atatggtgct ttaaaggata gtacaggata gttttgtttt tcatattcat
     2101 ttattttacg tcactataac gattgactta gaaactaaag tataaaataa cctattaatt
     2161 aatttatcaa atgcaagtat tactcggaat tatttttcac tccataggag gtttttcatc
     2221 aggaagtttt tatatgccct acaaaaaagt taggggatgg gcttgggaaa gctattggat
     2281 aattggagga tttttctctt ggttgattgt tccgccactt gccgcttatt tgaccgttcc
     2341 tggatttgtt gatattatca gtaatgctgc gccaactgta aaagcggtta cttatactat
     2401 gggattgatt tggggaattg ggggacttac ttatggtctt ggagttcgct atttggggat
     2461 gtcattgggt aattcgataa cattaggttt ttgttctgct tttggagcat tggttccttc
     2521 catatattat aacttttatc cagtagcagg caaaacctct tttaccgata tgttgcatag
     2581 cacaggcgga caaatggttt tgttgggggt tgtggtttgt ttagtaggta ttgccttttc
     2641 aggaaaagca ggaatgctta aagaaaaaga ttttgctaca ggtcatgaag ataaagacaa
     2701 agattttagc cttgtaaaag gtttgattat cgccataata tcgggtattt tgagttcttt
     2761 tttcaatttc ggaattgaag caggaaaacc aatggcagaa gaagccgttg ctcagggttt
     2821 taatccattg tttcaaaaca atgtaactta tatcgtaatc ctttgggggg gattgaccat
     2881 caatttttta tggtgtatgt atcttaattt taaaaacaaa acttttggag attataccaa
     2941 tagccaaact ccaataacta aaaatattat gttttcggct ttggcaggaa ccatgtggtt
     3001 tttgcaattc tttttttatg gaatgggcga aagcaaatta ggaaatggtg ctagttcatg
     3061 gattctacac atggcaacaa ttattttaac ggccaacttt tggggttttt acttaaaaga
     3121 atggacgggt gtttctaaaa aaaccttcaa tacatttctt ataggtattg gtcttatcct
     3181 tgcttcgatt gtaatcgtag gaatcggaaa ttcgctttaa acttataatt agtaacaata
     3241 ttaaatcaaa aacaatgtca aatataaatt caaacaacat gacttttaag cacgtaagct
     3301 acctatggga tgaagctaaa gccgcatcaa tggctggaga tgaagtagca ttatttattt
     3361 accgttctaa tttattagga gcggatttaa gattaacaaa ctacggaggt ggaaatactt
     3421 ctgtaaaaat aactgataaa gatccattga caggagccgc ttcagaagta atgtggatta
     3481 aaggttctgg aggagatatt gggacattaa ccaaatcagg ttgtgcggca ctttatttag
     3541 agagattgct taaccttgaa aatgtgtaca gaggaatcga attcgaagac gaaatggtgg
     3601 aattgttcaa ccactgtatt tttgatttag catcaaaagc accttctatt gataccccat
     3661 tgcacggttt cttgcctttc aaacacatcg atcacttgca cccagatgct gcaattgcaa
     3721 tcgctgctgc aaaagatggt aagaaaatca cagaagaatt attcaacgga gaaatcggtt
     3781 gggtgggatg gcaacgtcca ggttttgatt taggtcttca aatgcgtgct tgcttgcaag
     3841 aagctgaagc aaaaggaaaa aaactaaaag gagtaatgtt aggttctcac ggtttgttta
     3901 cttggggaga tacagcttac gaaagttaca tcaacacttt ggaagttatc gaaaaatgtg
     3961 ctgaatattt agagtctaac tacggaaaga ctcgtccagt ttttggaggt cagaaattag
     4021 aaagtcttcc agaggatgct cgccaattgc aagcagcaaa attagctcca atcttgagag
     4081 gtttttgttc ttcagagcgc aaaatgattg gacattatac agatgattca agagttatgg
     4141 aattcatcaa ttctaatgat ttagagaaat tagctcctct tggaacttct tgtccggatc
     4201 acttcttgag aactaaaatc agtcctttag taattgaatt ggctccaaac gaagatttat
     4261 cagatgtagc tgctatcaaa gcaaaattaa ctcctgcttt tgaggcttac cgtgaaatgt
     4321 acaaagagta tcacaatact tgcaaaaaat caaactcacc agctatgcgt gacccgaatc
     4381 cagttgtaat tttatatcct ggagttggta tgtttacttt tgcaaaagac aaaacaatgg
     4441 ctcgtttagc agctgaatat tataccaatg cggtaaacgt aatgaaaggt gctgaagcag
     4501 tttcagaata tacatcattg ccacgtcaag aagctttcga catcgaatat tggttgttag
     4561 aagaagctaa attgcaacgt atgccaaaac caaaagcatt atctggaaga atcgctgtta
     4621 ttacaggttc ggctggtgga attggaaaag ctatcgctaa aaaattcgcc gaagaagggg
     4681 cttgtgtagt tatcaatgac attaacgaag agcgtttaca aggcgcaact gctgatttcg
     4741 ttaaagcttt tggtaaagat gcagtttcta gtactttatt gaatgtaact gatgaaaaaa
     4801 gcactgaatt ggctttggat gctgcttgtt tagcatttgg tggagctgat attattgtga
     4861 acaatgctgg aatcagtatt tcaaaatcta tcgctgaaca ttcattagaa gagtgggata
     4921 gattatacga tatcttggtg aaa
//
LOCUS       DAOSNN010000207         4937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_17208_length_4937_cov_5.039533, whole genome shotgun sequence.
ACCESSION   DAOSNN010000207 DAOSNN010000000
VERSION     DAOSNN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17208_length_4937_cov_5.039533"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1116)
                     /locus_tag="PLQ70_09925"
     CDS             complement(175..1116)
                     /locus_tag="PLQ70_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQE34972.1"
                     /translation="MDNLNSNSIPKNSTGNSGVWWHENNPGPLANIPFIKQFGSMRFT
                     KVQMDENMRPHLNDGIEIHFVTSGKYDWVVEGNQIELLPGNLSVTAPWHWNGSPNGKM
                     DIGYINWIIIKPQEFSQKKNLNLGNWTKLSKSIQENLGNLIADKSNAVLEKAKNFEKY
                     FNDIRKELADQEVGFEIMVGNILENLLIDLYRQLSSIGQKSEEETNFIDKFTKIVSED
                     ISKKWNIDDLANQFGMGKTKFTYEVKRLTGYPPNSYIINLKIEKAIEMILNPKNKSMP
                     DIAYSCGFSSLQHFTSSFLQRTGTTPGKYRNKETKEN"
     gene            1375..>3691
                     /locus_tag="PLQ70_09930"
     CDS             1375..>3691
                     /locus_tag="PLQ70_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQE34973.1"
                     /translation="MNLKQQSKLFLAFFLIGMMLTANAAEIWVSTQGKDTNLGTKSSP
                     LATIQMAVRKARELRRLKDASIKDGIHIIVMDGTYNLSEPLFIRPEDSGTAESPTTIE
                     ADANAKPILSGGIEIKNWKKSTLAINGLKVGTVWEADAPQQAGEIINYRQLWVNGKKA
                     VRAKSTSGNKMDRILSWDAVTETCWIPFKDKSVKFEPGMEMFIVQWWAIANLRIKNIE
                     VVKDSARLSFEQPESRIQSEHPWPAPWISKNNGNSAFFLNNGISMLDEAGEWYLDRKK
                     AKIYYIPRAGENMASAKVTIPVLENLVEMKGTLDSPVHHFNFKGISFQYSNWLRPSQQ
                     GHVPLQAGMYLLDAYKLKTPGTPNQDNLENQAWVGRPRGAVEVNYANNTLFESCTFEH
                     LSSTGLDFHRGTNHNVVKGNLFKDIGGNGINLGVFSDEAIEAHLPLQVKDDREICSDE
                     MVSDNLITNVANEDWGCLGIAAGFVKNLTIEHNEISDVAYSGISMGWGWTHTENVMRN
                     NKIIGNKIHHYAKHLHDVAGIYTLSSQPDSRIEENYIDKVYNSPYAHDPFLWLYLYTD
                     EGTQGFTIKNNWIPIQKNLKNHNGPAGNIWQDNYPFVNPEIRKNAGIRAPYGNPNKEE
                     DKLSLQVVPKALASEYPIGNPKTKKNASNSASQGSLDKEVVVDEAWGLQEMPKAVAIE
                     LIGKNFDVETIKSTIKGFRIVGEELYQWENHLVIYGLMNQPERTKRKLALAFPAMDIK
                     IYENPVYDFQNFERCKDVKVASEWENIVLTAN"
     assembly_gap    3692..3701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3702..4015
                     /locus_tag="PLQ70_09935"
     CDS             <3702..4015
                     /locus_tag="PLQ70_09935"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HQE34974.1"
                     /translation="DDAKMQKEYVDYHKTQFEKWPEIAKGFCNADFQQLQVFKNGRQL
                     ILVISIPKGESLDKLNPKTTENNPRVNDWNNLMKKYQTGIEGAKPGETWIFLKKLEEK
                     K"
     gene            4106..>4937
                     /locus_tag="PLQ70_09940"
     CDS             4106..>4937
                     /locus_tag="PLQ70_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQE34975.1"
                     /translation="MLKIAILGLGEGRSTMSAALKSSKLELVKICDCNEELCKQRAKE
                     FDFHSYTTNYDDLLKDSAIDIIAIYTPDHLHAQHVKQALLAGKHVVCTKPFIDDLSDA
                     KELLALSKSSGKKVFIGQSSRFFEPAKRQRADFEAGLIGDLITIEAHYHADHRWFLAK
                     EWSLLQSFKWLYGGLSHPVDFIRWYLPNIEEVMGYGMISSNGKSAGLKNEDTMHFIFK
                     ATDGRIARVSGVYTSPTQPTKRDSGMSTILRATEGASQSDYHELRYAITDKTGEEKII
                     H"
BASE COUNT         1659 a          875 c          996 g         1397 t
ORIGIN      
        1 aatcagactt tattagtatt caaatataat aattttttct tacaaatcat caaaataatt
       61 ttttatttgt agaatacttt tcgtacttat tggggcagta aggttcaata attaaacttc
      121 ctttttcagg ttaataatgg atagaataat ctcatgttcg aaagcaaatt ccctctaatt
      181 ctctttcgtt tccttatttc tgtattttcc cggagtggtt cctgttcttt gcaaaaagga
      241 agaagtaaaa tgttgtaatg atgaaaaacc gcacgaataa gcaatatctg gcatcgattt
      301 gtttttaggg tttagaatca tttcaatggc tttttcaatt tttagattga tgatatagct
      361 attgggtgga tatccagtaa gtcttttgac ttcataagta aatttggttt tccccattcc
      421 aaattgattg gccaaatcat ctatattcca ttttttgctg atgtcttctg aaacgatttt
      481 ggtgaatttg tctataaaat tagtttcctc ttcggatttt tgtccaatgc tagaaagttg
      541 tcggtacaaa tcgattagta gattttctaa aatattaccc accataattt caaatcctac
      601 ctcttgatcg gctagttctt tgcgaatgtc attaaaatat ttttcgaaat ttttagcctt
      661 ttctaataca gcattactct tgtcagcaat caaattacct aagttttctt gaatgctttt
      721 ggataattta gtccaatttc caagattcaa attctttttt tgactaaatt cttgtggttt
      781 tataatgatc caattgatgt atccaatatc cattttgcca ttaggacttc cgttccaatg
      841 ccatggtgct gtaaccgata aattgccggg taataattca atttgattgc cctcgacaac
      901 ccagtcatat ttgccactgg ttacaaaatg aatttcgatt ccgtcattca agtgcggtcg
      961 catattttcg tccatttgca ctttggtaaa acgcatactt ccaaattgtt tgataaaagg
     1021 aatgtttgct aatgggccag gattgttttc gtgccaccaa acgcctgaat ttccggtgct
     1081 atttttgggt atgctatttg aatttaaatt atccataatt ttttatcaaa ttactaagtt
     1141 gatttctgag ggccaaggta caaaataatt atataattgt tcgttgctta aaaataacat
     1201 agaattatta tataaagatt acttttaaca tttaaataat taatctcaag ataaaatata
     1261 atattcacag aaatagatta ctataaatag gattaataaa taaaagggca aatgttatgc
     1321 aatatatgac gattcaaaaa tatatatttg tcaaattaac aattaataaa aacaatgaat
     1381 ttgaagcaac agtctaaact ctttttggct ttcttcctca taggaatgat gcttactgcc
     1441 aatgctgccg aaatctgggt ttctactcaa ggaaaagaca ccaaccttgg aacaaaatca
     1501 agtcctttgg caactattca aatggcggta agaaaagcca gggagctacg ccgattgaaa
     1561 gatgcgtcga taaaagatgg aatccacatc attgtgatgg acggaactta taatttaagc
     1621 gaacctttat tcataagacc tgaagattct ggaactgccg aaagtcccac taccattgaa
     1681 gccgatgcaa acgcaaaacc aatactaagc ggtggaatcg aaatcaaaaa ttggaaaaaa
     1741 tcgacacttg ccataaacgg tttaaaagtt ggaacggttt gggaagccga tgcgccacag
     1801 caagcgggcg aaatcatcaa ttacagacaa ttgtgggtca acggaaaaaa agccgtgaga
     1861 gccaaaagta cttcaggaaa caaaatggac agaatcctgt cttgggatgc tgtaacggaa
     1921 acctgttgga ttcctttcaa ggacaagtca gtcaagttcg agccaggaat ggaaatgttc
     1981 attgtgcaat ggtgggccat cgccaatttg cgaatcaaga atatcgaagt ggtcaaagat
     2041 agcgcccgac tttctttcga acaaccagaa agccgtattc aaagcgagca tccttggcca
     2101 gcgccttgga tttccaaaaa caacggaaac tcggcatttt tcttgaacaa cggaatttcg
     2161 atgttggacg aagccggtga atggtatttg gacaggaaaa aagccaaaat ttattacatt
     2221 ccaagagcgg gagaaaatat ggcttcggca aaagtaacga ttccggtttt ggaaaatttg
     2281 gtcgaaatga aaggaacttt ggattcgccc gtgcatcatt ttaatttcaa gggaatttcg
     2341 tttcaataca gcaattggct tcgtccttcg cagcaaggcc acgttccgtt gcaagccgga
     2401 atgtatttgt tggatgccta caaattgaaa acgccgggaa caccaaatca agataattta
     2461 gaaaatcagg cttgggtagg tcgcccaaga ggagccgttg aggtgaatta tgccaacaat
     2521 actttgttcg aatcttgcac ttttgagcat ttgtcctcca ccggactgga ttttcacaga
     2581 ggaacgaatc ataatgttgt caaaggaaat ttattcaagg acattggcgg caacggaatt
     2641 aatctaggtg ttttctccga cgaagctatc gaagcgcatt tgccattaca agtaaaagat
     2701 gatagagaaa tttgttcgga tgaaatggtt tccgacaatt tgattaccaa cgtggccaac
     2761 gaagattggg gatgtttagg aatcgcggct ggtttcgtaa aaaatcttac gattgaacac
     2821 aacgaaattt cggatgtggc ttacagcggg atttcgatgg gttggggttg gacacacacc
     2881 gaaaacgtga tgcgaaacaa caaaattatt ggcaataaga ttcatcatta tgccaaacat
     2941 ttacacgatg tagccggaat ttacacgctt tcatcgcaac cggacagccg aatcgaagaa
     3001 aattacattg acaaagttta caatagtccg tatgcacacg acccgttttt gtggttgtat
     3061 ttgtacaccg acgaaggaac acaaggtttt accatcaaaa acaactggat tccaattcaa
     3121 aaaaatctga aaaaccacaa tgggcctgct gggaatattt ggcaggacaa ttatcctttc
     3181 gtgaatcctg aaatcaggaa aaatgccgga attcgtgcgc cttacggcaa tccgaacaaa
     3241 gaagaagaca aattgagttt gcaagtagtg ccaaaagcgc ttgcctccga atatcctata
     3301 gggaatccca aaacaaagaa aaatgcaagt aatagtgcat ctcaaggtag tcttgacaaa
     3361 gaggttgtag tggacgaagc ttggggtttg caggaaatgc ccaaagcggt tgccatagaa
     3421 ttaataggga aaaattttga tgtggaaacg atcaaatcga ccataaaagg tttccgaatc
     3481 gtgggcgagg aattgtatca atgggaaaac catttggtca tttacggttt gatgaaccag
     3541 ccggaaagaa ccaaaagaaa actggctttg gcttttccag caatggatat caaaatctac
     3601 gaaaacccgg tttatgactt ccaaaatttc gaaagatgca aagacgtaaa agtcgcttcg
     3661 gaatgggaaa acatcgtttt gactgcgaat tnnnnnnnnn ntggatgatg caaaaatgca
     3721 aaaagaatat gtcgattatc acaaaaccca attcgaaaaa tggccggaaa ttgccaaagg
     3781 cttctgtaat gcagattttc aacaactgca ggtttttaaa aatggaagac aattaatctt
     3841 ggtcatcagt attccgaaag gggaaagttt ggataagtta aatccaaaaa caaccgaaaa
     3901 caatccacgt gtcaacgact ggaacaactt gatgaaaaaa taccaaaccg gaatcgaagg
     3961 ggcaaaaccc ggagaaacct ggatattttt gaagaaactt gaagaaaaaa aatagattaa
     4021 aatatttagc cacagattaa atggattaaa aaatccttaa tctgtggcaa aaaaaaaaaa
     4081 taataacagc gaataacaaa aagaaatgtt aaaaatagcc attttaggtc taggagaagg
     4141 tcgaagtaca atgtctgccg ctttaaaaag tagcaaatta gagttagtaa aaatttgtga
     4201 ttgcaatgag gaattatgca aacaaagagc caaggaattc gattttcatt cctacacaac
     4261 caattacgat gatttattaa aggatagcgc aatcgatatc atcgccattt acacgccaga
     4321 ccatttgcac gcccagcacg taaaacaggc tttattggct ggaaaacacg tagtttgtac
     4381 caagccattt attgatgatt tatcagatgc gaaagaatta ttagcgttga gcaaaagcag
     4441 tggaaagaag gttttcattg gtcaaagttc tcgatttttt gaaccggcaa aaagacaaag
     4501 agccgatttt gaagctggat taattggcga tttaattacc attgaagccc attatcacgc
     4561 cgaccaccgt tggtttttgg ccaaagaatg gtcgttgttg caatctttca aatggttgta
     4621 cggcggattg agtcatcctg tggatttcat ccgttggtat ttgcccaata tcgaagaagt
     4681 gatggggtac ggaatgatca gcagcaacgg aaaatcggca ggcttgaaaa acgaagacac
     4741 gatgcacttt attttcaagg cgaccgacgg cagaattgcc agagtgagcg gtgtttatac
     4801 ttcgccaacg caaccaacga aaagagatag tggaatgagc acgattttga gagccacgga
     4861 aggtgccagt caatccgatt accacgaatt gcgttatgcc attactgata aaacgggaga
     4921 ggaaaaaatc atacatt
//
LOCUS       DAOSNN010000208         4874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_17480_length_4874_cov_5.336792, whole genome shotgun sequence.
ACCESSION   DAOSNN010000208 DAOSNN010000000
VERSION     DAOSNN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17480_length_4874_cov_5.336792"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLQ70_09945"
     CDS             complement(<1..195)
                     /locus_tag="PLQ70_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorT family protein"
                     /protein_id="HQE34976.1"
                     /translation="MKKTHLITFALLLLAMNTQAQLLQIGVKAGLNYANFSETNIQTD
                     AITSYHAGVIAEVKLLDKFAI"
     gene            366..968
                     /locus_tag="PLQ70_09950"
     CDS             366..968
                     /locus_tag="PLQ70_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34977.1"
                     /translation="MRKIILLVVVLLFANGYSQEAGKFRVGLDFGYVPAGGGGGGLVS
                     LEPKFNIKDNMNVGLRLGGAAVVRDLVTNSNDEFTAKVAGISSFVGTYDYYFHKSGSS
                     FAPYIGGGVGYYSLANVELDDTDINEGSSTELNPAVSGVFGGLIRGGFEWAGFRMGLE
                     YNFLPESDIEDVNGAKVGTAKNAYLGIHIGFFVGGGKWGR"
     gene            1251..1724
                     /locus_tag="PLQ70_09955"
     CDS             1251..1724
                     /locus_tag="PLQ70_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE34978.1"
                     /translation="MKKIILSVALVFVFGFANAQSGKFKLGAHLGLPMGDIKDIRSLN
                     LGVDAAYVWNVAENFSAGIASGYTNYSGKSGFDSVGFIPIAATGQFSLSKSMFLGADL
                     GYGIYVGSGSGDGGFYYQPKLGYQTEKFEVYLGYKGISVDGGTFSSLNVGFNYKL"
     gene            1805..2701
                     /gene="xerD"
                     /locus_tag="PLQ70_09960"
     CDS             1805..2701
                     /gene="xerD"
                     /locus_tag="PLQ70_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HQE34979.1"
                     /translation="MNWKSDIKNYQSYLKIERGLSKNTIDNYSFDIERLCLFLDENNI
                     SVSPLKISEETIQQFIYSVSKEVNPRSQARIISGLKSFFSYLIFEDYRTDNPMELIET
                     PKTGRKLPDTLTVEEIDALIAAIDLSSNEGERNRAMLETLYGCGLRVSELITLKISDL
                     FFEEGFIKITGKGNKQRFVPVGKLTQKYIQIYRDYKRSELNIKKGQEDTLFLNRRGNQ
                     LTRAMVFTIIKNLTAAIGLKKSISPHTLRHSFATHLLENGADLRSIQLMLGHESITTT
                     EIYVHLDRKFLTEVINNFHPRG"
     gene            2881..4386
                     /locus_tag="PLQ70_09965"
     CDS             2881..4386
                     /locus_tag="PLQ70_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQE34980.1"
                     /translation="MIFSKDKNSDDLNYFYSKLFKETPDLIFQFSIDPFNKYTFSLIS
                     SSILQIVELSSEDFTNDNKQSIYDRIIQEDRDSFFQSLVYAKKSIGRWEPKFRVLLPI
                     KGLRWLKISARTEALDDGSVVFYGRVSDITEIKKQELELEISEERFQFALEASTAGVW
                     DWDLRTDKVFYSSQSLKILDLESTDVFDNPERWDKIVHPDDLAKYYSDIQKHFDNIVP
                     FYENYHRVLTANGKYKWILDRGKVIERDLDGKPLRVIGTHTDISAQKEKELELVRTTE
                     LFEEHNRRLLNFSYIVSHNLNTHAGNIKSLLDFIEIEEDNETKKEFLEHLRTVSNDLN
                     CTIANLSQIVDIQNNLNIKKEPLNLNDYLEKTLKVSNLYSGKKVLFKNRIPKDVVVNF
                     NSAYLESVLQNLTTNAIKYSHTERVCLIEYDFYVENGKKVLSISDNGLGIDLDKYGDT
                     LFGLYKTFHQHVKSRGMGLYITKNQIEAMNGSIGVESEVGKGTTFKITFEN"
     gene            complement(4495..>4874)
                     /locus_tag="PLQ70_09970"
     CDS             complement(4495..>4874)
                     /locus_tag="PLQ70_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022232.1"
                     /note="protein from Staphylococcus aureus has
                     phosphodiesterase activity against 2'-3'-cAMP and
                     2'-3'-cGMP; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQE34981.1"
                     /translation="KEEVCNAIGAHHDEIEMKSLLSPIIQVCDAISGARPGARRQVLD
                     SYIQRLKDLEEVAYGFGGVKNAYAIQAGRELRVIVESEKVSDENAATLSFEISQKIQT
                     EMTYPGQVKVTVIRETRAVNIAK"
BASE COUNT         1599 a          646 c          914 g         1715 t
ORIGIN      
        1 aatggcaaat ttatctagaa gtttaacttc tgctatcact ccagcatgat agctcgttat
       61 ggcatcagtt tggatgtttg tttcagaaaa gttggcgtaa tttaaaccag cttttacacc
      121 aatttgtaat aattgcgctt gagtattcat tgctaagagt agtaaagcga aggtaattaa
      181 atgtgtcttt ttcataatga tataaatttt gggttgcttt tccaacgcta aaattatttg
      241 attattgtaa aatttaactt tttactgatt tcgtatttgt taataacttg atgtgacgat
      301 ttattttatt aaaaagaaaa atactatttt taagaaaaaa ttacaaaaat taatcttaaa
      361 aaattatgag aaaaattatt ttattagttg ttgttttgct gtttgctaac ggctattcac
      421 aagaagctgg aaagtttaga gtaggtttgg attttggtta tgtgccagca ggaggaggtg
      481 gaggaggatt agtttctctt gagcctaagt ttaatataaa agacaacatg aatgtaggat
      541 tacgattggg tggagccgca gtagtccgtg atttggtgac caatagtaat gatgaattta
      601 ctgcaaaagt tgctggaatt agttcctttg taggtacgta tgattattat tttcacaaat
      661 caggaagttc ttttgcacct tatattggag gtggtgttgg ttattattct cttgcaaatg
      721 ttgaattgga tgatactgat attaatgaag gtagttctac tgaattaaat cctgctgtat
      781 ctggcgtatt tgggggttta attcgtggag gttttgaatg ggcaggtttt agaatgggtt
      841 tagagtacaa ttttcttcct gaatctgata tagaagatgt taatggagct aaagttggaa
      901 cagcaaaaaa tgcttattta gggattcata ttggattctt tgtaggtggt ggaaaatggg
      961 gtagataaca tcttgattga acatatagca tctaaccacg caatttttgc gtggtttttt
     1021 tgttttttat agctagtaaa acttgtgttt ttatattaat tagaaaacaa ttgtagaatt
     1081 attctatttg gcgagttgtg aatttttagt aaattaggat ttaataagat ttaaattgat
     1141 gatttaataa tatttgttat caattttgtt aataacttgg tttttaagtt aaaagcgctt
     1201 ccagtttaat gtctaactta gtagtattaa taactttaaa atatatgaat atgaaaaaga
     1261 ttattttatc agtagcgtta gtgttcgttt ttggatttgc taatgcacaa agcggaaagt
     1321 ttaaattagg agctcattta gggcttccaa tgggtgacat taaagacatt aggtcattaa
     1381 atcttggagt tgatgctgct tatgtttgga atgtagcaga aaatttttct gcaggaattg
     1441 catcaggtta caccaattac tcaggtaaat caggttttga ttctgttgga tttattccta
     1501 ttgctgccac aggccagttt tcattatcta aaagtatgtt tttgggtgca gacttagggt
     1561 atggaatcta tgttgggtca ggtagtggcg acggcggatt ttattaccaa ccgaaattag
     1621 gatatcaaac agaaaaattc gaggtttatc ttggatataa aggtatttct gttgatggag
     1681 gaacattttc ttcgcttaac gtaggattta attataaatt ataacctaaa aattcaatca
     1741 taattctgac catcagttta ggctgatggt ttttttatgc ccgaaaaatt cgtttcttta
     1801 cagtatgaat tggaaatcgg atatcaaaaa ttatcaatct tatttgaaaa ttgaaagagg
     1861 tttgtcgaaa aatactatcg ataattattc ttttgatatc gaacgattgt gtcttttttt
     1921 ggacgaaaat aatatttcag tttcaccatt aaaaattagt gaagaaacca ttcagcaatt
     1981 catttatagt gtttcaaaag aggtaaatcc tcgttcgcag gctagaatta tttcgggctt
     2041 aaaaagtttt tttagctatt taatcttcga agattatcga acagataatc caatggaatt
     2101 gattgaaacc cccaaaacag ggaggaaact gccagatact ttaacggttg aagaaattga
     2161 tgctttaatt gccgccatag atttaagttc gaacgaagga gaacgaaaca gggcgatgtt
     2221 ggaaacactt tatggttgtg gacttcgagt ttcggaattg attacgctta aaatttcgga
     2281 cttgtttttt gaagaaggtt ttatcaaaat tacgggtaag ggcaataaac aacgatttgt
     2341 tcctgtgggg aaattgacgc aaaaatacat ccaaatttat agagattata agagatctga
     2401 attgaatata aagaaaggcc aagaagatac tttgttctta aatagaagag gaaatcagtt
     2461 gacacgagca atggttttta caataattaa aaacctaact gctgcaattg gattaaaaaa
     2521 aagtatcagc ccacatacct tgcgacattc gtttgcgaca catctcctcg aaaatggcgc
     2581 cgatttgcgt tcgattcagt taatgttggg gcacgaatcg attacaacga cagaaatata
     2641 cgtgcattta gacaggaaat ttttgactga agtcattaat aattttcatc caagaggata
     2701 acttttttgt ataattaatt taaaaccata catttgtcta aaggtaactt ttttcgagat
     2761 ttattagtct catttgtatt gttaagataa tgctacttaa attgtttttt tagttttgtc
     2821 ttgtatttgg tgatgaagtt gtctgtaact tatttgtttt aatcttaatt taaaaatagt
     2881 atgatttttt ctaaggacaa aaattccgat gatttaaatt atttttacag caaattattt
     2941 aaagaaactc ctgatttaat atttcagttt tcaattgatc cgttcaataa atatactttc
     3001 tctttaataa gctcatctat tcttcaaata gtagaattat cttcagaaga ttttaccaac
     3061 gacaataaac aatctattta tgatagaata attcaagaag atcgtgattc tttttttcaa
     3121 tctttagttt atgcgaaaaa aagtattgga agatgggaac ctaagtttcg agttttgttg
     3181 ccaattaaag ggttaagatg gctcaaaatt tcagctagaa cagaggcctt agatgatggt
     3241 agtgttgttt tttatggtag agtttctgat ataactgaaa ttaaaaaaca ggaactagaa
     3301 cttgaaattt cggaggaacg attccagttt gctttagagg catctacagc tggagtatgg
     3361 gattgggact tgagaaccga taaagtgttt tattcttccc aatcattaaa aattcttgac
     3421 ttagaatcga cagatgtttt tgacaatcca gaacgttggg acaaaattgt tcatcccgat
     3481 gatttagcaa aatattattc ggatatccag aagcattttg acaatatcgt tccattttat
     3541 gaaaattatc accgggtttt gactgccaac ggaaaatata agtggatatt agatcggggt
     3601 aaagtaattg aacgagactt ggacggaaaa cctttgcgcg taattgggac tcatacagat
     3661 atttcggcac aaaaagaaaa ggaattagaa ctagtaagaa ctactgaatt gttcgaggaa
     3721 cataatagaa ggcttttgaa tttttcttat atagtctcac acaatttaaa tactcatgca
     3781 ggaaatatta aatcattgct cgattttatt gaaattgagg aagacaacga aactaaaaag
     3841 gagtttttag agcatttgag aaccgtttca aatgatttga attgtacaat tgctaatctg
     3901 tctcaaattg ttgatattca gaataatttg aatatcaaaa aagagcctct taatttgaat
     3961 gattatttag aaaaaacatt aaaggtttct aatttatatt ctggtaaaaa agtactattc
     4021 aagaaccgca ttcccaagga tgttgtagta aattttaatt cagcatatct tgagagtgtt
     4081 ttgcaaaatt taaccaccaa tgccattaaa tattcacaca ctgaaagggt ttgtttaatt
     4141 gaatatgatt tctatgtaga gaatggcaaa aaagtgctga gtatttcgga taatggcttg
     4201 ggtatcgatt tagataaata tggagataca ttatttggac tgtataaaac ttttcatcaa
     4261 catgtaaaat cacgcggaat ggggctttat attactaaaa accaaataga agctatgaat
     4321 ggttcaatag gagttgaaag cgaagttggc aaaggcacta cttttaaaat tacttttgaa
     4381 aattaatctt tcaaataagt ccagaaaata aaaaattcca aataccaata taactattgg
     4441 aatttggaat ttttatttcc actccctctc cttagagagg gctggggtaa ggatttattt
     4501 agcgatatta accgctctgg tttctctaat tacagtaact tttacttgac ctggataagt
     4561 catttctgtt tggatttttt gagaaatttc aaaagataaa gttgctgcat tttcatctga
     4621 tactttctcg ctttctacaa taacgcgaag ttctctaccg gcttgaatgg cataagcatt
     4681 tttcacgcca ccaaatccat aagctacttc ttctaaatct ttcaaacgtt ggatataaga
     4741 atctaaaact tgtcttcttg cgcctggtct cgcaccagaa atagcatcac aaacctgaat
     4801 gattggagat aacaatgatt tcatttcaat ttcgtcgtgg tgtgctccaa tggcgttgca
     4861 aacttcttct tttt
//
LOCUS       DAOSNN010000209         4742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_18042_length_4742_cov_4.946448, whole genome shotgun sequence.
ACCESSION   DAOSNN010000209 DAOSNN010000000
VERSION     DAOSNN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18042_length_4742_cov_4.946448"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PLQ70_09975"
     CDS             <1..908
                     /locus_tag="PLQ70_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQE34982.1"
                     /translation="QKKLKGKKSLAATLIVLCILGIVILPSISFFSNVTSGILEIKTG
                     IDEGTLIISKPGQNIKDWPIIGEKVYAFLDSASTNLEKTFIDHKDQIKEIATKVVGNL
                     VSSIVTILQIVLSVIIAGVLMASTDAQNLATNFIMRIAGDKGDEYIEIFVSTVRQVVK
                     GVIGVAVIQTMIQAVGLFMCGVPFPGILTLLCLILSIIQVGPIIVNIGVIAYLFSTGD
                     SSTAAIFWTVYFILSGLSDNVLKPLLLGKGALVPMLVIFIGVIGGFMMSGFIGLFVGP
                     IVFSIGYKLFIAWMEDKPEVEVKIN"
     gene            1184..3691
                     /gene="nirB"
                     /locus_tag="PLQ70_09980"
     CDS             1184..3691
                     /gene="nirB"
                     /locus_tag="PLQ70_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HQE34983.1"
                     /translation="MIKVIVVGNGMVGYKFCEKFITKAGSEKYQITVFGEEPRRAYDR
                     VHLSEYFAGKSADDLSMSTANWYDDNNIILNTSELITDINREQKTIHTHLEKTHEYDY
                     LVLATGSAAFVPPIEGVDKEGVFVYRTIEDLDAIMSYAKKIKQKGATEAAVLGGGLLG
                     LEAAKAVRDLGLNPHVVEFAPRLMPRQLDKGASDMLQSKIEALNIGIHLNKSTQYIAG
                     EDAITGMMFEGDDLLKVDMLVVSAGIKPRDELGRVSGLEVGVRGGVVVNNKMQTSDPY
                     IYAIGEVALYNHMIYGLVAPGYEMADVAAEQIIGNEKLMRETIDMSTQLKLIGVEVAS
                     FGDPFIENDEVTAIVYENKFSGIYKRINVTKDGKTLLGGILVGDSSDYNGLFQIYSNA
                     MKLPANPEDLILGSRGGEGSTMGSAMDLPDSAVICSCENVTKGSICCSITDGSCETFS
                     DVVKATKATSGCGGCKPMVVDLVKAAQKSIGKEVKESLCEHFNYTRQELFDIVKINKY
                     TNFYEVIDHHGNGDGCEVCKPVVASIFSSIYNDTANKHVTTQDTNDRYLANIQRNGTY
                     SVVPRIAGGEITPEKLIVIGEVAKQFNLYTKITGAQRVDLFGAELNDLPKIWKILIEN
                     GFESGHAYGKSLRAVKSCVGNAWCRYGMDDSTTLAIELENRYRGIRSPHKLKGGVSAC
                     IRECAEARGKDFGVIAVEGGWNLYICGNGGANPKHAVLLAEQIDKETVFKYMDRFLMY
                     YIRTAGPLVRTSTWLEKLDGGLDYLKEVIIHDRIGICEALEAEMAALVDTFECEWKQV
                     LEKPRLMKRFSHFVNSEEKDDNIEYVGLRDQKMPRPW"
     gene            3799..4176
                     /gene="nirD"
                     /locus_tag="PLQ70_09985"
     CDS             3799..4176
                     /gene="nirD"
                     /locus_tag="PLQ70_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HQE34984.1"
                     /translation="MENIVSQYKSVAVEDVNIWFKAGTLKDFPSNRGGCIKYKNKQIA
                     VFNFERRNEWYACQNACPHKMEMVLSRGMTGSADGVPKIACPMHKKTFSLVDGSNLNG
                     EDYSIATYPVKVVDGEVFVGFLE"
     gene            4212..>4742
                     /locus_tag="PLQ70_09990"
     CDS             4212..>4742
                     /locus_tag="PLQ70_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HQE34985.1"
                     /translation="MTPFQKASEWIDAENAQDPNQEIYQSQTYPKELLYSNRMYERLM
                     DFYPNASEEIQIAAKAQHICRWKMPRESYPMDRVGYLKWREDLKKFHAKTTAEILAKA
                     GYLQKFIDRVSFLIEKKLLKKDEETQLLEDVICLVFLEYYFDPFVQKHDREKMKNIVL
                     KTWNKMSETGHQEALKI"
BASE COUNT         1630 a          689 c          976 g         1447 t
ORIGIN      
        1 ttcaaaaaaa gttaaaagga aagaaatctt tagctgcaac tttgattgtt ctatgtattt
       61 tggggatagt tattttgcca tccatttcat tttttagtaa tgtaacttcg ggtatattgg
      121 aaattaaaac tgggattgat gagggtactc taattataag caaaccagga caaaacatta
      181 aagattggcc aattatcgga gaaaaagttt atgctttttt ggattcagcc tctactaatt
      241 tagaaaaaac atttatagat cataaagatc aaattaaaga aatagctaca aaagttgttg
      301 gaaatcttgt cagttctatt gtgaccatat tgcaaattgt cctttctgtt attattgccg
      361 gagttttgat ggcttctact gatgctcaga atttagcaac caattttatt atgagaatag
      421 caggagataa aggagatgag tatatagaga tttttgtttc tactgttcgt caggtagtca
      481 aaggagtgat aggagtagcc gtgattcaaa ctatgattca ggcagtggga ttatttatgt
      541 gtggcgttcc gtttccggga atattgacat tgttgtgctt gatactttca attatacaag
      601 taggaccaat tattgtcaat attggtgtga ttgcctatct tttttccact ggagatagtt
      661 cgacagctgc tattttttgg actgtatatt ttattttgag tggactatca gataatgttt
      721 tgaagccgct tttattaggt aaaggagctt tggttcctat gcttgttata tttattggtg
      781 tgataggagg atttatgatg tcaggattca ttggcttgtt tgtaggacca attgtgtttt
      841 ctataggcta taaattattt attgcttgga tggaagataa accagaggtt gaggttaaaa
      901 tcaattaagt aataatgcga atcaagggtt gaatatctga tgaactggtt taagaaaatc
      961 taaaagtttt ttttaaacct ttgaggttta gttttagtac tatactttcc ataattttaa
     1021 aaccgtaata cgtttaaaat aaatgtatta cggttttttt atgcaataga ttcaaaacta
     1081 ttttcatttt ttttcaaaat ttattttaaa aatattcaaa attaagtatt taaattaagt
     1141 attaatacgt atatttgcgt aataatactt aattaatgca attatgatta aagttatagt
     1201 agtcggaaac ggcatggtag gttataaatt ttgtgaaaaa ttcatcacaa aagcaggaag
     1261 tgaaaaatac caaattacgg tatttggaga agaaccaaga cgtgcttatg atagagtaca
     1321 tcttagtgaa tactttgcag ggaaaagtgc agacgatttg tcaatgtcaa ctgcaaattg
     1381 gtatgacgat aacaatatta ttttaaacac atctgaatta attacagata tcaatagaga
     1441 acaaaaaacc atacatacac atttagagaa aacacatgag tatgactact tagttttggc
     1501 tacgggttcg gctgcttttg ttcctccaat tgaaggggtt gataaagaag gggtttttgt
     1561 ttatagaacc atcgaagatt tagatgcaat tatgtcttat gccaaaaaaa taaaacaaaa
     1621 aggagccact gaagctgccg ttttgggtgg cggattgtta ggacttgagg ctgctaaagc
     1681 ggtacgagat ttgggattaa atcctcacgt agtagaattt gcccctcgat tgatgccaag
     1741 acaattagac aaaggtgcta gtgatatgtt gcaatctaaa atcgaagcac taaacattgg
     1801 tattcatctt aataagtcaa cacaatatat cgctggagaa gacgctatca ctggaatgat
     1861 gttcgaaggt gatgatttgc ttaaagtaga tatgttagtg gtttctgcag ggattaaacc
     1921 tcgagatgag ctaggaagag tttcagggct tgaagttggt gttcgtggtg gagttgtggt
     1981 aaataataaa atgcaaacat cggatcctta tatttatgct attggtgaag tagcacttta
     2041 caaccatatg atttacggac ttgtagctcc aggttatgaa atggcagatg tagctgcaga
     2101 gcaaatcatt ggtaacgaaa aactaatgag agaaaccatc gatatgtcaa cgcaattgaa
     2161 attaataggt gttgaagtag ctagttttgg tgatccattt atcgaaaacg acgaagtaac
     2221 cgctatcgtt tatgaaaata aattcagtgg aatctacaaa agaatcaatg ttaccaaaga
     2281 tggaaaaaca cttttgggtg gaattcttgt aggcgattct agcgattaca acggattgtt
     2341 tcaaatttat agcaatgcga tgaaattgcc agcaaatcca gaggatttaa tcttgggttc
     2401 aagaggaggt gaaggttcta caatgggaag cgcaatggat ttgccagatt cggctgtgat
     2461 ttgttcttgt gaaaacgtga ccaaaggaag catttgttgt tctatcactg atggtagttg
     2521 cgagactttc tcagatgtag taaaagccac aaaagccact tcgggttgtg gtggttgcaa
     2581 accaatggtg gtcgatttgg taaaagcagc tcaaaaatca attggaaaag aagtaaaaga
     2641 aagcctttgt gaacacttta attacaccag acaagaatta tttgatattg taaaaatcaa
     2701 taaatacact aatttctatg aagtaattga tcatcatgga aatggtgacg gttgcgaggt
     2761 ttgcaagcct gttgttgcat ccattttttc gagtatttac aacgatacgg caaacaaaca
     2821 cgtgactacc caagacacaa atgaccgtta cttggctaat attcaacgaa acggaactta
     2881 ctcagtagtg ccaagaattg ctggaggcga aattactcca gagaaattaa ttgttattgg
     2941 cgaagtagcc aaacaattca atttatatac aaaaatcaca ggagcacaac gcgtcgattt
     3001 gtttggtgct gaattaaacg atttgccaaa aatttggaaa atattgattg aaaatggttt
     3061 cgaaagtggt cacgcttacg gaaaatcatt gagagccgta aaaagttgcg tgggtaatgc
     3121 ttggtgtcgt tatggtatgg acgatagtac taccttggcc atcgaactag aaaatcgcta
     3181 ccgcggaatc cgttctccac acaaattaaa aggaggcgtt tcggcttgta tcagagaatg
     3241 tgccgaagct agaggaaaag attttggggt aattgccgtt gaaggaggtt ggaatcttta
     3301 tatctgtgga aatggtggtg ctaatccgaa acacgcggtg cttttggccg aacaaataga
     3361 caaagaaact gttttcaaat atatggatcg ttttctaatg tattatatcc gcactgctgg
     3421 gcccttagta agaacatcaa cttggttaga aaaactagac ggaggattag attatcttaa
     3481 agaagtaatc attcacgacc gcatcggtat ttgtgaagct ttagaagccg aaatggcagc
     3541 attagttgat acttttgaat gtgaatggaa acaagttctt gaaaaaccaa gattgatgaa
     3601 acgttttagc cacttcgtga attctgaaga aaaagacgat aatatagaat acgtgggatt
     3661 aagagaccaa aaaatgccta gaccttggta attaaataat taatactatt ctttccaaag
     3721 ctacttcaac aattttaaga tctttcgcaa tcctaaaaaa agttgaagta gtggaaagat
     3781 atcaaacaca taaaaatcat ggaaaatata gtaagccaat acaaatcagt agccgtagaa
     3841 gatgtaaata tttggttcaa ggcaggaacc ctaaaagact ttccaagtaa tagaggaggt
     3901 tgcatcaaat ataaaaacaa acaaattgct gttttcaatt tcgaaagaag aaacgaatgg
     3961 tatgcttgtc aaaatgcttg tcctcataaa atggaaatgg ttttgtcaag aggaatgact
     4021 ggttcagccg atggtgtgcc aaaaattgct tgtccaatgc acaaaaagac cttttcgctc
     4081 gtggatggtt ctaatcttaa tggtgaagat tattctatcg ccacttatcc tgtaaaagtg
     4141 gtcgatggag aagtattcgt tggctttttg gaataattgt gtatctttga atttcaacaa
     4201 ttcaaataaa aatgacacca tttcaaaaag ctagtgagtg gatagatgcc gaaaatgcac
     4261 aagatccaaa ccaagaaata taccaatccc aaacctatcc aaaggaactt ttatattcca
     4321 atagaatgta tgaaagactg atggattttt atccaaatgc ttctgaagaa attcaaatag
     4381 cagccaaggc acaacatatt tgccgatgga aaatgccaag agaatcctat ccaatggatc
     4441 gtgtaggtta cctaaaatgg cgtgaagatt tgaaaaaatt tcacgccaaa actactgctg
     4501 aaatcctagc caaagcagga tatttacaaa aatttatcga tagagtttct tttcttatcg
     4561 aaaaaaaatt actcaaaaaa gacgaagaaa cccaactttt ggaagacgta atttgtctcg
     4621 tgtttttaga atattatttt gatcctttcg ttcaaaaaca cgatagagaa aaaatgaaaa
     4681 acatcgtact aaaaacctgg aataagatgt cagaaacggg gcatcaagaa gccttaaaaa
     4741 tc
//
LOCUS       DAOSNN010000210         4726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_18131_length_4726_cov_4.412331, whole genome shotgun sequence.
ACCESSION   DAOSNN010000210 DAOSNN010000000
VERSION     DAOSNN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18131_length_4726_cov_4.412331"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PLQ70_09995"
     CDS             complement(<1..148)
                     /locus_tag="PLQ70_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HQE34986.1"
                     /translation="MKNLFDNTQIAFSLKSDTELERAFFLFKMIANEPLVRIGTAVTN
                     FALKA"
     gene            241..1308
                     /gene="aroB"
                     /locus_tag="PLQ70_10000"
     CDS             241..1308
                     /gene="aroB"
                     /locus_tag="PLQ70_10000"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQE34987.1"
                     /translation="MQTIQANNYPIYFNEKGYEVLNSFIKEGKYSNVFIIVDSNTNEY
                     CLSKFLPYLATELTIEIVEFEAGEPNKNIETCVQLWNVLTELGADRKSLVLNLGGGVV
                     TDLGGFVASTFKRGVDFIHIPTTLLSMVDASVGGKTGVDLGNLKNQIGVINVPKMVLI
                     DTQYLETLSKEEMRSGLAEMLKHGLIYDKGYWEQFLDLKAIDFADFDELIYRSVEIKN
                     EIVMQDPTEKNIRKALNFGHTLGHAIESYFLENENKKSLLHGEAIAIGMILESFISLE
                     KNLINPQEYNQIKTAIKGIYDDVVFEENDIEPIMELLIHDKKNEYGNIQFALIEGIGK
                     IKINQSVENELILKAFQDYKS"
     gene            1599..2999
                     /locus_tag="PLQ70_10005"
     CDS             1599..2999
                     /locus_tag="PLQ70_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HQE34988.1"
                     /translation="MNTKYFDLINQTYYFPQEEFTLNKDNLEFHNIDLMKLVEQYGTP
                     LKFTYLPQISNNINKAKSWFRKSMEKNNYEGKYYYCYCTKSSHFEYIMNEAFKNNIHI
                     ETSSAFDINIVESLLESGKINKSTYVICNGFKRDQYIENIARLINNGHKNTIPIIDNY
                     EELDILQENIKKKFKIGIRIAAEEEPKFEFYTSRLGIGYKNIVQFYKKQIQDNKKLEL
                     KMLHFFINTGINDNAYYWNELVKCIKVYVALKKECPTLDGLNIGGGFPIKNSLAFEYD
                     YQYMIDEIINQIKIACDEAEVDVPNIFTEFGSFTVGESGGAIYQILYQKQQNDREKWN
                     MIDSSFITTLPDTWAINRRFIMLSINRWNDTYERVLLGGMTCDSDDYYNSEQNMNAIY
                     LPKYNKEKPLYIGFFNTGAYQETIGGYGGLHHCLIPQPKHILIDRDENGILATEVFSQ
                     QQTADEVLEILGYNKK"
     gene            3080..4051
                     /locus_tag="PLQ70_10010"
     CDS             3080..4051
                     /locus_tag="PLQ70_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HQE34989.1"
                     /translation="MSKGPISQFIEKHYLHFNSASLVDAAKAYEQQLANGAKMMVSMA
                     GAMSTAEIGKIFAEIIRKDKVQIISCTGANLEEDIMNLVAHSHYERVPHYRDLTPEDE
                     WALLERGLNRVTDTCIPEHEAFRRLQKHIYKIWKDADDKGERYFPHEFMYKMLLSGVL
                     EEYYEIDLKDSWMYAAAEKNLPIIVPGWEDSTMGNIFASYVIKGDLKASTMKSGIEYM
                     TFLADWYPKNSANGIGFFQIGGGIAGDFPICVVPMLYQDMEMHDIPFWSYFCQISDST
                     TSYGSYSGAVPNEKITWGKLDIKTPKFIIESDATIVAPLIFAYLLDL"
     gene            4060..4470
                     /locus_tag="PLQ70_10015"
     CDS             4060..4470
                     /locus_tag="PLQ70_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQE34990.1"
                     /translation="MKRVIVDYAKLTHEILNLLVDKFPDGYDDSNIIRFRNAQNELIE
                     AVEVRTEDTIYLVKISTKLADRIENYDEDDEIAAEIEPIEEGIIDDDDAVSDDDDDTD
                     EYKDPVDKGDDDDDDDDDDEEADSSEEEDEDDEE"
BASE COUNT         1736 a          691 c          822 g         1477 t
ORIGIN      
        1 ttgctttgag agcaaaattt gttacagcag ttccaattcg aacaagtggt tcattagcaa
       61 tcattttgaa tagaaaaaag gctctttcga gttctgtatc gctttttaat gaaaaggcaa
      121 tttgtgtatt atcgaataaa tttttcatta tttgttatat ttttacacaa atatagagag
      181 agttttcaaa attattaagc aaaaaatacc ttattttgca ctttaaatta atccaagatt
      241 atgcaaacca ttcaagccaa caattatcca atatatttta acgaaaaagg atatgaagtt
      301 ctaaactctt tcatcaaaga aggcaaatat tctaacgtat ttattattgt agatagcaat
      361 acaaatgaat attgtttgtc taaatttctt ccttatttag caaccgaatt gaccatagaa
      421 attgttgaat ttgaagccgg tgaacccaat aaaaacattg aaacgtgtgt tcaattatgg
      481 aatgttttga ccgaactagg tgctgataga aaaagtcttg ttctaaatct gggtggcgga
      541 gttgttactg atttaggcgg ttttgtagct tcaacattca agcgtggagt agattttatt
      601 catataccaa ccactttatt atcaatggtt gatgcttcgg ttggaggaaa aactggtgtt
      661 gatttaggaa atttaaaaaa tcaaattggg gttatcaatg ttccaaaaat ggttcttatt
      721 gatactcaat atttagaaac actttctaaa gaagaaatgc gttctggact tgccgaaatg
      781 ctcaaacacg gattgattta tgacaaaggc tattgggaac aatttctaga tttgaaagcc
      841 attgattttg ccgattttga tgaattgatt tatcgttcgg ttgaaattaa aaacgaaatt
      901 gtaatgcaag atccaaccga aaaaaatatt cgcaaggcat tgaattttgg tcacacactg
      961 ggtcacgcca tcgaaagtta ttttcttgaa aatgaaaaca agaaaagtct attgcacggc
     1021 gaagctattg ctataggaat gattttagaa agttttattt cgttagaaaa aaacttgata
     1081 aacccacaag aatacaacca aatcaaaact gcaataaaag gcatttatga tgatgttgtt
     1141 tttgaagaga atgatatcga acccataatg gaattgctca ttcacgacaa aaaaaatgag
     1201 tacggaaaca ttcagtttgc gttaattgaa ggtattggaa aaataaaaat caatcaatct
     1261 gttgaaaatg aattgatttt aaaggctttc caagattata aatcttaaca atttttataa
     1321 aaaaggattg taattggtat atattatttt ctacatttgc aacaatgaaa aaaagtcaaa
     1381 atacaaggaa ttatagcgct ctaaaaaacg aaaaaagaag ttctttttta agaatattaa
     1441 ggcgtttttt tgctgttaag gattctttta gttttgatat ttttccctat ttgaaagccg
     1501 aaaacgaaaa acttcaaatc gtttttgaaa acattagagt ttgaatttta gcttaattaa
     1561 ttatttcaat taaattaatc taaaatatta aaaatcaaat gaatacaaaa tatttcgatt
     1621 taataaatca aacttactat tttcctcaag aagaatttac attaaataaa gacaacctag
     1681 aatttcataa tatagatttg atgaaattgg tagagcaata tggtacgcca ttaaaattta
     1741 cctatttgcc tcaaatttcc aataacatta acaaagccaa aagttggttt cgtaaatcga
     1801 tggaaaaaaa caattatgaa ggcaaatatt actactgtta ttgcacaaaa agttctcatt
     1861 ttgaatacat aatgaatgaa gctttcaaaa ataatattca tatcgaaacc tcttcggctt
     1921 ttgatattaa tattgttgaa agtttattag aatctgggaa aataaataaa agtacttatg
     1981 taatatgtaa cggtttcaaa agagatcaat acatcgaaaa cattgcacga ttaatcaata
     2041 atggacataa aaataccatt ccaattattg ataattatga agaattggat attttgcaag
     2101 aaaatatcaa aaagaaattc aaaatcggaa ttcgaattgc agctgaagag gaacctaaat
     2161 ttgagtttta tacttcacgt cttggaattg gttataaaaa catcgttcag ttctataaaa
     2221 aacagattca ggataataaa aaactggaac ttaaaatgtt gcactttttt ataaacaccg
     2281 gaatcaacga caacgcctat tattggaacg aattagtaaa atgtatcaaa gtgtatgtcg
     2341 ctttgaaaaa agaatgccct acacttgatg gtttgaacat tggtggcgga tttccaatca
     2401 aaaattcatt ggcattcgaa tacgattacc aatacatgat tgacgaaatt atcaatcaaa
     2461 ttaagattgc ttgtgatgag gccgaagtag atgttccaaa catatttact gaatttggtt
     2521 cttttaccgt tggtgaaagt ggtggtgcga tttatcaaat tttgtatcaa aaacaacaaa
     2581 atgacagaga aaaatggaat atgattgatt cctctttcat tactacttta cctgatactt
     2641 gggctataaa cagacgtttt attatgttat ccatcaaccg ctggaatgat acttacgaaa
     2701 gagttttact aggcggaatg acttgtgata gtgatgatta ttacaattca gaacaaaata
     2761 tgaacgccat ttatttgcca aaatacaaca aagagaaacc gctatatatc ggttttttca
     2821 acacaggagc ctatcaagaa actataggcg gttatggagg tttgcaccac tgtttgattc
     2881 cgcaacccaa acatattttg attgatcgtg acgaaaacgg aatcttagcc accgaagttt
     2941 tctcacaaca acaaaccgcc gatgaagtat tagaaatatt aggctacaac aaaaaataat
     3001 ttttaaaatt tttcattgag ttatcaaaaa aaaatatttt ctttgaacac tcaggaatcc
     3061 gcttttaaac aataaaaaaa tgagtaaagg accaataagt cagtttatcg aaaagcatta
     3121 tttgcatttc aattctgcat ctttagtcga tgccgccaaa gcctacgagc aacaattagc
     3181 taacggagct aaaatgatgg tgagcatggc tggagcaatg agtacagccg aaataggcaa
     3241 aatttttgct gaaatcattc gcaaagacaa agtgcaaatc atttcttgta caggagcaaa
     3301 tcttgaggaa gacatcatga atttagtagc acattcgcac tacgaaagag taccacatta
     3361 tcgtgattta actcctgaag acgaatgggc attactagaa agaggtttaa atcgtgttac
     3421 cgatacttgc attccagagc acgaagcttt ccgtcgtttg caaaaacaca tctataaaat
     3481 ttggaaagat gccgacgata aaggggaacg ctattttcct catgaattta tgtacaaaat
     3541 gctactttcg ggagttttgg aagaatatta cgaaattgac ctaaaagaca gctggatgta
     3601 tgctgcagca gagaaaaatt tacctattat tgtaccaggt tgggaagata gcacgatggg
     3661 aaacattttt gcttcctatg ttatcaaagg cgatttgaaa gcatctacaa tgaaatcagg
     3721 tattgaatac atgactttct tggcagattg gtatccaaaa aatagcgcta acggtattgg
     3781 atttttccaa attggtggtg gaattgctgg agactttcca atttgtgttg taccaatgct
     3841 gtatcaagat atggaaatgc atgacattcc gttttggagt tatttctgcc aaatttcaga
     3901 ttctacaacc agctatggtt cgtattcggg agctgtgcca aatgaaaaaa tcacttgggg
     3961 taaattagac attaaaaccc ctaagtttat aatcgagtcg gatgcaacca ttgttgctcc
     4021 attaattttt gcctatttgt tggatttata atataattta tgaaaagagt aattgttgat
     4081 tacgcaaaac ttacccacga aattttaaat ctattagtag ataaatttcc ggatggctat
     4141 gatgattcaa atattatccg ttttagaaat gcccaaaatg aattgatcga agctgttgaa
     4201 gttcgtaccg aggacactat ttatttagtg aaaatcagta ccaaattggc cgatagaatc
     4261 gaaaattatg atgaagatga tgaaatcgca gcagaaattg aaccaatcga agagggcatc
     4321 atagacgatg acgatgctgt cagtgatgac gatgatgaca cagatgaata caaagaccca
     4381 gtggataaag gcgatgacga tgacgacgac gatgatgacg atgaagaagc cgattcatct
     4441 gaggaagagg atgaggatga cgaagaataa tttattcttc aaaataatac tatttaaaag
     4501 gaactcttaa tggagttcct ttttttgtta aaactatctt gctgatacac agaaattata
     4561 ccaaacgaat ttatgtttta gtccaaagtg ttctggtata atgtcgatat agtattaaaa
     4621 tagtcctcca gaaacttcgg gatcattgaa ctatatcgaa tacacaaaaa atattcaaca
     4681 aataaaaagt ccttttgatg atttgtaaga aaaaattatt atattt
//
LOCUS       DAOSNN010000211         4698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_18254_length_4698_cov_7.369158, whole genome shotgun sequence.
ACCESSION   DAOSNN010000211 DAOSNN010000000
VERSION     DAOSNN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18254_length_4698_cov_7.369158"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLQ70_10020"
     CDS             complement(<1..798)
                     /locus_tag="PLQ70_10020"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQE34991.1"
                     /translation="MKLAVVGATGMVGEIMLKVIEETNFPYTELILVASEKSVGKVIE
                     FKGQKHTVVGLQTAVDMKADIAVFSAGGQTSLDWAPKFAAAGTTVIDNSSAWRMDPTK
                     KLIVPEINANELTKDDKIIANPNCSTIQMVLALAPLHKKYNIKRVVVSTYQSITGTGV
                     KAVQQFENECAGIEGDMVYKYKINRNCIPQCDSFEANGYTKEEMKLSNETKKILGDNC
                     VAVTATAVRVPVVGGHSEAVNVEFSNDFDVNEVRTILSQTEGIVVQDN"
     gene            complement(968..1384)
                     /gene="mscL"
                     /locus_tag="PLQ70_10025"
     CDS             complement(968..1384)
                     /gene="mscL"
                     /locus_tag="PLQ70_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQE34992.1"
                     /translation="MGFFKDFKASLMKGDVLSLATAVVIGGAFGKIVGSAVDDVIMPI
                     VGLITGGIDFTQKFITLDGNSYDNLAAAKTAGAAVITYGNLVQAVINFVIISFFIFVV
                     LRAAEKAKKKEEAAPAAPAGPTQEELLTQIRDLLKK"
     gene            complement(1444..3885)
                     /locus_tag="PLQ70_10030"
     CDS             complement(1444..3885)
                     /locus_tag="PLQ70_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HQE34993.1"
                     /translation="MTLSIQNIEKMLHAKRFGDNNGIIDTISIDSRSLQNNQKTLFFA
                     LVGSNNDAHIYISDLIKKGVQNFVVTHIPEGLANKAVFLVVENTLDALQKFAAHHRSL
                     FKFPVFGLTGSNGKTIVKEWLNFLLNPDFNIIRSPKSYNSQVGVPLSVISINDKHNLG
                     IFEAGISKMDEMEKLENIIRPTIGIITNIGTAHDEGFEDLEKKIKEKLKLFKHSKLII
                     YQKNKIIDALIKSKIKSFSWSFNDDTADVFISKKEIQDKTTLQVRYGKENFDVQIPFQ
                     DEASVENAATCLMVLLSFKYDKKTIQNRMQWLYPVEMRLKVKNGINKTTLIDDSYSCD
                     FQSLKIALDFLESQKQYKNKTLILSDIFQSGLSNEELYTKVAQLLKNNKVNRVIGIGE
                     TISAFKNKFTNCTTFKTTADFFLNFNYLNFVNETILIKGARHFQFEEIVSALEEKTHE
                     TILEINLNSISHNLSFFKSKLKPTTKMMVMVKAFGYGSGGFEICKLLEHHKVDYLGVA
                     FADEGISLKNAGITLPIMVLNPETTSFSAIIQYKLEPEIYSLKGLNAFLKIAENRKLK
                     HFPIHIKLDTGMHRLGFEEENLDELIAILKENKFVTVKSILSHMATSDDFEHQEFAHS
                     QIDLFEKLSSKLMNELQIKPIRHILNTSGISNFPQAQYDMVRLGIGLYGVSNDAEEQK
                     YLENVGTLKSIISQIRTIQAGESVGYGRRFVADKPTKIATIPIGYADGISRHWGNGVG
                     FVTINNQKATIVGSICMDMLMVDVSEINCKEGDSVIIFGESPSVSFIAEQLQTIPYEI
                     LTSISQRVKRIFYRE"
     gene            complement(4095..4685)
                     /locus_tag="PLQ70_10035"
     CDS             complement(4095..4685)
                     /locus_tag="PLQ70_10035"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HQE34994.1"
                     /translation="MFLENTVNHKEQFGWIEVICGSMFSGKTEELIRRLKRAQFAKQK
                     VEIFKPEIDTRYHDEMVVSHDANEIRSTPVPAAANIAILAQGCDVVGIDEAQFFDDEI
                     VKICNDLANQGIRVIVAGLDMDFKGNPFGPMPALMATAEYVTKVHAVCTRTGNLANYS
                     HRKTDSDKLVLLGETEEYEPLSRAAYFYAMKKDEEK"
BASE COUNT         1434 a          862 c          767 g         1635 t
ORIGIN      
        1 gttatcttga actacaatac cttcggtttg gctaagaatt gttctaactt cgttcacatc
       61 aaaatcattg ctgaattcca catttacggc ttcgctatga ccaccaacaa caggcacacg
      121 aacagcagtt gcagtaaccg caacacaatt atcacctaat atttttttag tttcgttcga
      181 taatttcatt tcttctttgg tgtaaccatt ggcttcaaaa ctatcgcatt gaggaataca
      241 gttgcggttg attttgtatt tataaaccat atcgccttca attccagcac attcgttttc
      301 gaattgttgt accgctttta cacctgttcc tgtgatggat tggtaggtag aaacgaccac
      361 acgtttgata ttgtattttt tgtgcaaagg cgctaaagct aaaaccattt gaattgttga
      421 acagtttgga ttagcgatga ttttatcatc tttggtcaat tcgtttgcgt tgatttcagg
      481 aacaattaat tttttggttg gatccattct ccaagccgaa gaattgtcga ttactgtagt
      541 tcctgcagca gcaaattttg gtgcccaatc taaggatgtt tgacctccag ccgaaaaaac
      601 agcaatatcg gctttcatat ccacagcggt ttgcaatcct actacagtgt gtttttgtcc
      661 tttgaattcg attacttttc caactgattt ttcagaagca accaaaatta attcggtgta
      721 aggaaaattt gtttcttcga ttacttttaa cattatttcg ccaaccattc ctgtggctcc
      781 tacaactgca agtttcatat gtgtattttt taaattttag ttctaatttt gagatgcaaa
      841 aataaggaat aatggaatga aaacaagcgt gttcacaaaa aataacaaat tgttatattt
      901 ataacaatat aaaaaagaac cacgcttata ggcgtggttt agttagaata tataatgtag
      961 cggaaattta ttttttaagc aaatctctga tttgtgtaag caattcttct tgagttggac
     1021 cagctggtgc agcaggagca gcttcttctt tctttttcgc tttttcggca gctctcaata
     1081 ctacaaaaat gaagaatgaa ataatcacaa aattaataac ggcttgcaca aggttaccat
     1141 aagtaattac agcagctcca gctgtttttg cagcggctaa attatcataa ctgtttccgt
     1201 caagtgtaat aaatttttgt gtaaaatcta ttccacctgt aattaaacca acaattggca
     1261 taataacatc atcgactgcc gagccaacaa tttttccaaa tgcccctcct attacaaccg
     1321 cagttgctaa acttaaaacg tctcctttca ttaaagaagc cttaaaatct ttgaaaaatc
     1381 ccataattcc cctgtttttt agttaaaatt aatacgaata taattaattt attttaaaaa
     1441 aacttattct ctataaaaaa ttctttttac cctttgtgaa atactggtca gaatttcata
     1501 aggaatggtt tgcaattgtt cggctatgaa agaaacgctt gggctttcgc caaaaataat
     1561 aacagaatca ccttctttgc aattgatttc gctaacatca accatcaaca tatccataca
     1621 aatgctgcca actatggttg ctttttgatt atttatggtt acaaaaccaa caccatttcc
     1681 ccaatgtctt gaaattccat cggcgtaacc aattgggatt gtagcaattt ttgtgggttt
     1741 gtcggcaaca aatcgtcttc cataacccac gctttcacca gcttgaatag ttctaatttg
     1801 ggaaataata gattttaatg taccaacgtt ttccagatat ttttgttctt cagcgtcatt
     1861 agaaacacca taaagtccaa tgccaaggcg aaccatatcg tattgtgctt gtggaaaatt
     1921 actgattcca gaagtattta agatgtgacg aatgggtttt atttgtaatt cattcatcaa
     1981 ttttgacgac aatttttcga ataaatcaat ctgcgaatgg gcaaactctt gatgttcaaa
     2041 atcatcgctt gtggccatat gggacagaat actttttacg gttacgaatt tattttcttt
     2101 taaaattgca attaattcgt ccaaattttc ttcttcaaaa cccaaacggt gcattccggt
     2161 gtcgagtttg atgtgaattg ggaagtgttt tagttttcga ttttcggcaa ttttcaagaa
     2221 tgcattcaag cctttcaaac tataaatctc aggttctaat ttatattgta taatcgccga
     2281 aaaactcgtg gtttcaggat ttaaaaccat tatgggcaaa gttattcccg catttttcaa
     2341 cgaaattcct tcatcggcaa aagccactcc caaataatct actttgtgat gttcgagaag
     2401 tttgcaaatt tcaaatccgc cgcttccata accaaaagcc tttaccatta ccatcatttt
     2461 ggtcgtgggt tttaatttcg atttaaaaaa actcaggtta tgactaatgg aattcagatt
     2521 gatttccaga atagtttcgt gtgttttctc ctctaaagcc gaaacaattt cttcaaattg
     2581 aaaatgtctg gctcctttga tcaaaatggt ttcattgaca aaattcaaat aattgaaatt
     2641 cagaaaaaaa tcggccgtgg ttttaaaagt agtgcagtta gtaaatttgt ttttgaatgc
     2701 cgagatggtt tcgccaatac caattacacg atttacttta ttgtttttta acaattgagc
     2761 cactttggta tataattctt cattagacaa accgctttga aaaatatccg aaagaatgag
     2821 tgttttgttt ttgtattgtt tttgactttc aagaaaatcc aaagcaattt tcaacgattg
     2881 aaaatcgcag ctgtaactat catcaataag ggttgttttg ttaattccgt ttttgacttt
     2941 taagcgcatt tcaactggat acaaccactg catacggttt tgaatggttt ttttatcgta
     3001 tttaaaactc aataaaacca tcaaacaagt agctgcattt tctaccgaag cctcgtcctg
     3061 aaaaggaatt tgcacatcga aattttcttt gccataacga acttgaagcg tcgttttgtc
     3121 ttgaatttct ttttttgaaa taaaaacatc agcagtatca tcgttgaaac tccaagaaaa
     3181 tgattttatt ttagatttga ttaaggcatc aataatttta tttttttgat aaatgatgag
     3241 tttcgaatgc ttaaaaagtt ttagtttttc cttgattttt ttctccaaat cttcaaaacc
     3301 ttcatcgtgt gcagtcccaa tattggtgat gattccgatg gttggacgga tgatgttttc
     3361 caatttttcc atctcatcca tttttgaaat tcccgcttca aaaatgccta aattgtgctt
     3421 gtcatttatc gaaattaccg acaacggaac accaacttgc gaattatagc ttttaggact
     3481 tcgaatgata ttgaaatcag gattcaaaag aaaattcagc cattctttaa cgatggtttt
     3541 tccgttgctt cccgtcaaac cgaaaacagg aaatttaaac aaactgcgat gatgcgctgc
     3601 aaatttttgc aaagcatcca aagtgttttc gacaactaag aaaactgctt tattggctaa
     3661 tccttcagga atgtgtgtaa cgacaaaatt ttgaacccct ttttttatta agtccgaaat
     3721 ataaatatga gcgtcattat tggacccgac taaggcaaaa aataacgttt tttgattgtt
     3781 ttgcaatgaa cggctatcga tagaaatagt atctattatt ccgttgttat ctccaaacct
     3841 tttggcatga agcatttttt cgatgttttg gatagataaa gtcaagagtc taactttttt
     3901 tggagtgtaa ttaaatattc agtgcttgtc attactggaa ttacgcgttt ttaaataaaa
     3961 aaaaacgatt ctcaataatg acttatttgt atgtataaaa gtacataata aaagcttaca
     4021 agaaataaaa ttggcttttt tctaacaaaa aataatttct gaattctatg ttttttttag
     4081 agtatcttag gaaattattt ctcctcatcc tttttcatcg cataaaaata ggcagctcga
     4141 ctcaaaggtt cgtattcttc ggtttcgccg agtaaaacaa gtttgtcgct atccgttttt
     4201 ctatggctgt aattggccaa atttcctgtt cgagtacaaa cagcgtgcac tttggtcaca
     4261 tattcggcag tggccatcaa ggcaggcatt ggaccaaaag gattgccttt gaaatccata
     4321 tccaatccgg caacaataac acgaatacct tgattggcca aatcgttgca aatttttacg
     4381 atttcgtcat caaaaaactg agcttcgtca atgccaacta cgtcgcaacc ttgtgctaaa
     4441 attgcaatat tggcagcagc gggaactggc gtcgaacgaa tttcattggc atcgtgagac
     4501 actaccattt cgtcatgata acgggtgtcg atttcgggtt tgaaaatttc tactttttgt
     4561 ttggcaaatt gcgctctctt aagtctgcga atgagttcct cggttttgcc cgagaacatt
     4621 gaaccgcaaa taacttcgat ccaaccaaat tgttctttat gatttactgt attttcgaga
     4681 aacattttgt atttttct
//
LOCUS       DAOSNN010000212         4619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_18680_length_4619_cov_4.974365, whole genome shotgun sequence.
ACCESSION   DAOSNN010000212 DAOSNN010000000
VERSION     DAOSNN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18680_length_4619_cov_4.974365"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLQ70_10040"
     CDS             complement(<1..763)
                     /locus_tag="PLQ70_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LETM1-related biofilm-associated protein"
                     /protein_id="HQE34995.1"
                     /translation="MINPSAIGWIDKFFLKQKSSLLPIETHPNTFYKKVRETGFIYGH
                     IVSFDTAFEIEKKGWLQSEISKVALLNTLFRIYQLTTNDNNPDEFVSKAVEFYNQMNP
                     QGFNLLRKVLPVGTLSLDLEKIIDTRVQTNVDIISKNFSHIVTNALLFIDVLAFRQYL
                     IHGEIPEKYLKKIEEAIISIVSLALKTKTTKSKYDDLLIKLFEASVRYSKFSKVNVQS
                     QNFGMDELKLDYFTHILEKQYLVDMAGMALWSDGIV"
     gene            complement(815..1384)
                     /locus_tag="PLQ70_10045"
     CDS             complement(815..1384)
                     /locus_tag="PLQ70_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HQE34996.1"
                     /translation="MDKKEQHYFKNAKEWREWLHENHTKSTGICLIFYKVGSTFESMR
                     WEEAVQVAICYGWIDSTVKKMDDERRKQVFSPRKDKSVWSKLNKTYIEKLLKENLIHE
                     SGLAKIEIAKQNGSWNSLDAVEELIIPEDLKMAFEQNEIAFENYKKFSPSYRKSYLYW
                     LNQAKREETRSSRIVQIINFCQQNKKSRE"
     gene            complement(1401..1928)
                     /locus_tag="PLQ70_10050"
     CDS             complement(1401..1928)
                     /locus_tag="PLQ70_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HQE34997.1"
                     /translation="MKKIIITAAIVLAILIGCKSKGNSDDSKKLNLAFESKSNSNVTG
                     TATFTEKKGMVTFVAKLTGLKPGIHAIHIHEKSDCTAADGSSAGGHWNPTFKKHGKWG
                     VEEHHKGDIGNFTADEKGNGTITLTTDEWCIGCEDETKNILGKGLIVHQGADDFTSQP
                     AGNAGARVSCSAIIK"
     gene            2131..2880
                     /gene="modA"
                     /locus_tag="PLQ70_10055"
     CDS             2131..2880
                     /gene="modA"
                     /locus_tag="PLQ70_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQE34998.1"
                     /translation="MYKLKINTKITFLALLFSLNIQAQTVKVAAASNLRFVLDEIKEK
                     YEMAHPKAKIIINLGASGALVQQIANGADFDLFMAADKVFPEKLKAEGIVTGKIQTYA
                     FGKLVIWSNTIDLSKGLNCVANSDVKRIAIAKPEIAPYGKRAIECLKYYKLFDKVKTK
                     IVYADNIAQTAQFAQTGNAEIGFLALSLAMAPEMKGTYFVLDTKSYKPVEQAMVLIKS
                     WQKNPEAAKFVKFVLSNQCKPIFEKYGFLVP"
     gene            2883..3296
                     /locus_tag="PLQ70_10060"
     CDS             2883..3296
                     /locus_tag="PLQ70_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HQE34999.1"
                     /translation="MNILTGTITQIQQSGAILLVDIDVNGQNFSALLIESAIRPDWLQ
                     KSNEIDLVFKETEVSLAKKMSGLISLRNRMQCKVIQVERGELLSKITMQFQQQIISSA
                     ITTRAVDSLQIVVGDSVEAMVKSNEISVMKTKKLT"
     gene            3300..3980
                     /gene="modB"
                     /locus_tag="PLQ70_10065"
     CDS             3300..3980
                     /gene="modB"
                     /locus_tag="PLQ70_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009389851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HQE35000.1"
                     /translation="MDAAFVQTLWITFKLAFSTTVILILIGLPISYWLAYSRFKLKPL
                     VEALVSMPMVLPPSVIGYYMLVLYSPRNFFGKWLAETFDLRLAFTFEGVLIASVLFSL
                     PFMIQPLQTGLRSLPESLREASYTLGKSKTTTFFKVLLPNIKPSIITAIALTFAHSIG
                     EFGIVLMVGGNMPGETRVASIAIYDEVQSLNFAAANQYAFILFAVSLVLLTTIYSVNK
                     KHDGWKIM"
     gene            3965..>4619
                     /locus_tag="PLQ70_10070"
     CDS             3965..>4619
                     /locus_tag="PLQ70_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQE35001.1"
                     /translation="MENNVIAIDIEKKMFTVNGEINLAIQTSIKMGELVALFGSSGAG
                     KTTLLRILAGLVTPDKGIIKFGETVWFDSGNRINIPPQNRNISLMFQDYALFPNMTVE
                     GNIQFAQSEKDDQAVDELLSVFGLNEFRKHKTNGLSGGQKQRVALARALARKPQLLLL
                     DEPLSALDATMRASLQDEIVQAHQLLGATTIMVSHDLSEVFRLSNQVLCIENGIIQKS
                     "
BASE COUNT         1417 a          791 c          840 g         1571 t
ORIGIN      
        1 cgacaattcc atcactccac aatgccattc cagccatatc aaccagatat tgtttttcta
       61 agatatgagt aaaataatcc aatttcaatt catccatacc aaaattttgg gattgcacat
      121 ttactttcga aaatttgctg taacgaactg aggcttcgaa taattttatc aataaatcat
      181 cgtattttga tttggtagtc ttggttttca acgctaacga aacaatgcta ataatggctt
      241 cctcaatttt ttttaaatat ttttcaggaa tttcaccgtg aatcaagtat tggcggaaag
      301 ccaaaacatc tataaacaac aaagcattgg ttacaatatg agaaaagttt ttgctaatga
      361 tatcaacatt tgtttgaact ctagtgtcaa taattttttc taaatccaaa gaaagagttc
      421 caactggcaa tacttttcgt agtaaattga acccttgcgg attcatttgg ttataaaatt
      481 caactgcttt cgacacaaat tcatcgggat tattgtcgtt tgtggtcaat tgataaattc
      541 taaacaaagt attgagcagg gctactttag aaatttcgct ttggagccaa cctttctttt
      601 caatttcaaa tgcagtatca aatgaaacaa tatgtccata aatgaaccca gtttccctaa
      661 cttttttgta aaatgtgttt gggtgtgttt cgataggaag caaagaggat ttttgcttca
      721 agaaaaattt gtctatccaa ccaattgccg aagggttaat cattgtttta atttgtcttg
      781 caaatctata cctttttatg gttttgagta aaaattattc tctagatttt ttattttgtt
      841 ggcaaaaatt gatgatttgt actattctgc ttgatcgagt ttcttctctt ttggcttggt
      901 ttaaccaata aagataactt ttcctatagg aaggactgaa ttttttataa ttttcaaaag
      961 cgatttcatt ttgctcaaaa gccattttta aatcttctgg aattatcaat tcttcaacag
     1021 catccagcga attccaagaa ccattttgct tggcaatttc aattttggcc aacccacttt
     1081 cgtgaataag attctctttt agtagttttt cgatataggt tttgttgagt ttgctccaaa
     1141 cacttttgtc ttttcggggc gaaaaaactt gttttctgcg ttcatcatcc atttttttta
     1201 cggttgaatc aatccaaccg tagcaaatgg caacctgaac ggcttcttcc caacgcatac
     1261 tttcaaaggt actgcccact ttgtagaaaa ttaggcaaat tcccgttgat tttgtatggt
     1321 tttcgtgcaa ccattctcgc cattctttgg catttttaaa atagtgttgt tcttttttat
     1381 ccaaataatt gaaattatat ttatttaata attgccgaac aagacactct agctccagca
     1441 ttaccagcag gttgtgaagt aaaatcatca gcgccttgat gaacaattaa tcctttgcct
     1501 aaaatgtttt tggtttcgtc ttcacatcca atgcaccatt catcagtggt tagagtgatg
     1561 gttccgtttc ctttttcatc agcagtaaaa ttaccaatat cgcctttatg gtgttcttca
     1621 actccccatt tgccatgttt tttgaaggtt ggattccaat gtccaccagc cgaacttcca
     1681 tcagcagcgg tgcaatctga tttttcgtga atgtgaatgg cgtgaattcc tggttttaaa
     1741 cccgttaatt tagcaacaaa agttaccatt cctttctttt cagtaaatgt agcagttcca
     1801 gttacattac tattgctttt ggattcgaat gcaaggttca atttcttaga atcgtcagaa
     1861 tttccttttg atttacagcc aattaaaatg gctaatacta ttgcagcggt gataattatt
     1921 tttttcatag tttttggaat tagaatgtta tacaatatta ggcatttcta tggaatgaaa
     1981 taattaaagt tatcttaaaa agggttgctt tttatgaaat ggatatgcta atttagcatt
     2041 cagtgaaact taacaataaa atctagagtt agcaactcaa ttttaaaggg ataaaacggc
     2101 ttcaatacta gaacctaatc ttaattttga atgtataaat tgaaaatcaa taccaaaatc
     2161 acatttttag ctcttttatt ttctttaaat attcaggctc aaaccgtaaa agtagccgca
     2221 gcatctaact tgcgttttgt tctcgatgaa ataaaagaga aatacgaaat ggctcatcct
     2281 aaagcaaaaa tcattataaa tttgggagcg tctggagcat tagttcagca aattgcaaac
     2341 ggggccgatt tcgatttatt tatggcagct gataaagtgt ttccagagaa gttgaaagca
     2401 gaaggaatcg ttacaggtaa aattcaaacc tacgcttttg gaaaattagt catttggagc
     2461 aataccatcg atctgtcgaa agggttgaat tgtgttgcca attctgatgt gaaaagaatt
     2521 gcgattgcca aaccagagat tgctccatat ggtaaaagag ctatcgaatg tttgaaatat
     2581 tacaaacttt ttgataaggt aaaaactaaa attgtgtacg ccgacaacat tgcccaaaca
     2641 gctcaatttg cccaaactgg aaatgccgaa attggatttt tagcactttc tttagcgatg
     2701 gctccagaga tgaaaggcac ttattttgtg cttgatacta aaagttacaa accagttgaa
     2761 caagcaatgg ttttaattaa aagttggcaa aaaaatcccg aagcagctaa gtttgttaaa
     2821 tttgttttga gcaatcaatg caaacccatt tttgaaaaat acggattttt agttccttaa
     2881 ttatgaatat actaacaggt acaataacac aaatacaaca atctggggca attttgctag
     2941 tagatattga tgttaatggg caaaattttt cggctttgct tattgagtct gcgattagac
     3001 cagattggtt gcaaaagtcg aatgaaatag accttgtatt caaggaaact gaagtgtcac
     3061 ttgccaaaaa aatgtcgggt ttaatcagtt tgcgaaatcg gatgcaatgc aaagtcattc
     3121 aggtggaacg tggcgaattg ttgagcaaaa taacaatgca atttcagcaa caaatcattt
     3181 cgtctgccat tacgactcgt gcggtcgatt cacttcaaat agtggttggc gattctgttg
     3241 aagcaatggt caaatccaac gaaatttcag tgatgaaaac taaaaaactc acctaaatta
     3301 tggatgctgc ttttgttcaa actttgtgga ttacgtttaa attggctttt tcgacaactg
     3361 taatattaat tctaattgga ttgccaattt catattggtt ggcttattct cgatttaaat
     3421 tgaaaccttt agtagaagct ctggttagta tgccaatggt tttgcctccc tccgtaattg
     3481 gttattatat gttggtttta tacagtccac gaaacttttt cgggaaatgg ctcgccgaaa
     3541 ctttcgattt gcgattggct tttactttcg aaggtgtttt gattgcttcg gtacttttta
     3601 gtttgccttt tatgattcaa cctttacaaa cgggactccg ttcgttgcca gagagtttgc
     3661 gtgaagcatc ttacacatta ggaaagtcaa aaacgactac ttttttcaag gtattgcttc
     3721 ctaacataaa accgtcaatt atcacggcaa tagcgcttac ttttgcccat agtattggcg
     3781 aatttggaat tgtgcttatg gttggtggca atatgccggg cgaaactcgt gtggcttcga
     3841 ttgctattta tgacgaagtg caatcactca attttgctgc tgccaatcaa tatgctttta
     3901 tcctttttgc tgtatcgctt gtattgttaa ctacgattta cagtgtcaat aaaaaacacg
     3961 atggatggaa aataatgtaa ttgcgattga catcgagaaa aagatgttta ctgtaaatgg
     4021 tgaaatcaat cttgctattc aaaccagtat caaaatgggc gaattagtag cattatttgg
     4081 ttcatctggt gcaggaaaaa caacactttt acggattttg gcagggcttg taactcctga
     4141 caaaggtatt atcaagtttg gcgaaacggt ttggtttgat tcaggaaacc gtatcaatat
     4201 tccgccacag aatagaaaca taagtttgat gtttcaagat tatgcgctgt ttccgaatat
     4261 gactgtcgaa ggaaatatac aatttgcaca atctgaaaaa gacgatcaag cagttgacga
     4321 attgttgtcg gtttttggtt taaatgagtt tagaaaacac aaaaccaacg gattgtcggg
     4381 tgggcaaaaa cagcgagttg ctttggctcg tgcattggct cgaaagccac aattattact
     4441 tttagacgag ccactttcgg ctctagatgc tacaatgcgt gctagtttgc aagacgaaat
     4501 tgttcaagca catcaattat tgggagctac aacaattatg gttagccacg atttgagcga
     4561 agtttttcga ttgagtaatc aggtgctttg cattgaaaac ggaatcattc aaaaaagtg
//
LOCUS       DAOSNN010000213         4598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_18808_length_4598_cov_5.558221, whole genome shotgun sequence.
ACCESSION   DAOSNN010000213 DAOSNN010000000
VERSION     DAOSNN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18808_length_4598_cov_5.558221"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /gene="ric"
                     /locus_tag="PLQ70_10075"
     CDS             complement(<1..648)
                     /gene="ric"
                     /locus_tag="PLQ70_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HQE35002.1"
                     /translation="MSLQEITTIGDFVAKDFRTAAIFTKYGIDFCCKGQRTIDEVCRK
                     MAIDEDLLLEELNVVLATKNDSGVDFSSWPLDLLIDYIEKTHHRFVEEKTHMLMPLLD
                     KLCSVHGDNHPELFEINTLFKESAKELAAHMKKEELILFPFVKEMINATNSHGTIGKP
                     QFETVKNPIVAMMNEHEAEGERFRTISALTHNYTTPEDACNTYKVTYALLEEFEQD"
     gene            1001..1435
                     /locus_tag="PLQ70_10080"
     CDS             1001..1435
                     /locus_tag="PLQ70_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQE35003.1"
                     /translation="MLSKSQARAFFLGGTLVTFLIFIGLTVYSFMPSNDQTNYSSIDK
                     NVVRGKEIWESNNCMGCHTILGEGAYYAPELTKVIDRRGEAYVKVVLMSKEPWQPRGR
                     KMVAYGMNEADADAVVAYFKWIGKIDLNGFDRVVSPLSKVNN"
     gene            1447..2784
                     /locus_tag="PLQ70_10085"
     CDS             1447..2784
                     /locus_tag="PLQ70_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQE35004.1"
                     /translation="MKYKSQKVAYWFFALCMLLFSLQIIYGFIMGFDRIGLQGLHNIV
                     PFNVARAVHTNLLVVWLLTGFMGAAYYIIPEEAQRELISVKWAYIQLISLAVVGVVAV
                     VGFHLNHWEGRKFLEIPRELDFLVVINVLLFLGLILGTLFKGKRQTTTALVLSMGLLF
                     AALLYLPGMIWFDSQVMDSFFRWWVVHLWVEGVWELIMGGILSFLLIKLTGVDREVIE
                     KWLYVIVGLTFLSGVLGTGHHYYYIGVNKIWLIVGGIFSALEPLAFLAMALFAVNMYR
                     KGEKSHPNKIALFWTIGSAIVSFIGAGLLGFAHTLPQVNLYTHGTLVTAMHGHYAFWG
                     AYAMIVFAIISYALPNLTGRKRYDSTQGHLAFWLSNIGILGMTVAFGVAGVAQVYLER
                     KMKMEYGDVQNEIAVHFVVLLICATLFTVGISMFIYDFIKYGRPTDEALDENQ"
     gene            2902..3681
                     /locus_tag="PLQ70_10090"
     CDS             2902..3681
                     /locus_tag="PLQ70_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HQE35005.1"
                     /translation="MLENTPYYHAVGKEIEVFNQAYKNKIPFLLKGPTGTGKSRFVEY
                     MAHQLNKNIITISCHEETSSTDLIGRFIIKGAETIWLDGPLTTAVKEGAIIYLDEVAE
                     ARPDVIVAIHSLTDHRRELFIDKLGETVKAHDDFMLVASFNPGYQRGFKELKPSTRQR
                     FIAVSFDYPEPKIEIEILINETNIDQDTAKKLVAIGNKIRNLTELGLTETVSTRLLVD
                     AAKIIHSGLPKRLSVHVAIVEPLTDDLQTVEALKDLCNLMI"
     gene            3747..>4598
                     /locus_tag="PLQ70_10095"
     CDS             3747..>4598
                     /locus_tag="PLQ70_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NorD protein"
                     /protein_id="HQE35006.1"
                     /translation="MGFEIDEYIVGKFFKHLKRIKKVNPEISARTISLNDIKPRLTIL
                     ARAFTGKPIEIFPAEREGGYKNNNFFLPISFAEFSTIEENRTFYLFRILYLTIQQRLD
                     LNWKAEENEPTLSLSQQKAEETSHQILSVLFEEFPITKEIYNKAKRHFEQKIDSKTGV
                     DYSWIYGKWMRNEAEQKTDSVLENFSDKTKKPNENQALTTLKAKAVEEVITVELDKKQ
                     QEDYVLLHNFEKAETAEEFNGTWRDFDGSDELEDHQEALEELNMKYTVRVDDTSHSVY
                     QADFIENT"
BASE COUNT         1426 a          764 c          897 g         1511 t
ORIGIN      
        1 atcttgttcg aattcttcta atagtgcata ggttacttta taggtgttgc aagcgtcttc
       61 gggcgttgtg taattatggg tcaaagccga gatagttcta aatcgttctc cttcggcctc
      121 atgttcattc atcatggcaa ctataggatt ttttactgtt tcaaattgtg gttttcctat
      181 agttccgtga ctgtttgtgg cgtttatcat ttcttttacg aatggaaaca aaatcaattc
      241 ttcctttttc atgtgtgcag ctaattcttt tgcactttct ttaaaaaggg tattgatttc
      301 gaataattca ggatgattgt ctccgtgaac actacataat ttatctaaaa gaggcattag
      361 catatgtgtt ttttcttcga caaaacgatg gtgcgttttt tctatataat cgattaataa
      421 gtctaaaggc caagagctaa aatcgactcc cgaatcattt tttgttgcca aaacgacatt
      481 taattcttct aataaaaggt cttcgtctat cgccattttt cggcaaactt catcgatggt
      541 tctttgtcct ttacagcaaa aatcgattcc atattttgta aatattgcag ctgtcctaaa
      601 atccttggcg acaaaatctc caatggttgt tatttcttgt aaactcatga ttttttattt
      661 aatagaggat ttaaattcaa tgaattagga aaaaaaaaat actccttttt ttcgagtata
      721 attctacaga ttaaaagcat tatttcatat gatttaaatc atatttcttt ttatttatca
      781 tttttttatt aacacaaggt tgataaaatt tgaattctaa tctgcatcaa tttttatatt
      841 tttttgttcg aaatggtgtc tcaataattt ttttaatgaa tatgattgca atcataagat
      901 acaaattatt tcgatgctta ctttgcataa aataaataat ggatgatttt gtccgctatt
      961 taaataagta aatttattaa ctaacaaaaa actgataatt atgctttcaa aatcacaagc
     1021 aagagcattt tttctgggag gaacattggt caccttttta atttttatag gactgactgt
     1081 ttattcattt atgcccagta atgatcaaac caactacagt tctatcgata agaacgtagt
     1141 taggggaaaa gaaatttggg aatccaacaa ttgtatgggt tgtcatacca ttctgggaga
     1201 aggggcttat tatgctccag aattgaccaa agttatcgac cgtcgtggcg aggcttatgt
     1261 aaaagtagtc ttgatgtcta aggaaccttg gcaaccacgt ggacgaaaaa tggtggctta
     1321 cggaatgaac gaagccgatg ccgatgccgt tgttgcttat tttaaatgga ttggcaaaat
     1381 tgatttaaat ggttttgacc gtgtggtttc tcctttatct aaagttaata attaaattat
     1441 atcaaaatga aatataaatc acaaaaagta gcctattggt tttttgcatt gtgtatgctt
     1501 ttgttctcct tgcaaattat ctatggtttt attatgggct tcgaccgcat tggattgcag
     1561 ggattacaca atattgttcc gtttaacgtg gctcgtgcgg ttcatactaa tttattggta
     1621 gtatggttgt taacgggttt tatgggagca gcatattaca ttattcccga agaagcacag
     1681 cgcgaattaa tcagcgtaaa atgggcttat attcagctta tttccttggc ggttgttggt
     1741 gttgtagccg ttgtcggttt tcacttgaac cattgggaag gaaggaaatt ccttgaaatc
     1801 ccaagagaat tagacttttt agtagtaata aatgtattgc tttttctagg gttaatttta
     1861 ggaacgctat tcaaaggaaa acgccaaaca acgacagctc tagtgttgtc aatgggatta
     1921 ttgtttgccg ccttacttta tttgccagga atgatttggt ttgatagcca agtaatggat
     1981 tccttcttcc gttggtgggt agttcacttg tgggttgaag gtgtttggga attaattatg
     2041 ggaggtattc tttctttctt gttaattaaa ttgacgggag tcgatagaga agtgatcgaa
     2101 aaatggttgt acgtaatcgt tggattgact ttcctttctg gagttttggg aacaggacac
     2161 cactattatt atataggagt aaacaaaatt tggttgattg tgggtggtat tttctctgct
     2221 ttggaacctt tggctttctt ggcaatggca ttgtttgccg ttaatatgta tcgaaaaggt
     2281 gaaaaaagtc atcccaacaa aatcgcttta ttctggacca ttggttcggc tatcgtttct
     2341 ttcattggag ctggattgct tggttttgca cacaccttgc cacaagtaaa cctttatact
     2401 cacggaacct tggtaacggc aatgcacggt cactacgcat tttggggtgc ttatgcaatg
     2461 atcgtttttg ccataattag ttatgcgctt cctaatctaa cgggtcgaaa acgttacgat
     2521 agtacccaag gtcatttggc tttttggttg tccaatatag gaattttggg aatgacggta
     2581 gcctttggtg ttgctggtgt tgcccaagta tatttggaac gaaaaatgaa aatggagtat
     2641 ggtgatgttc aaaatgaaat tgccgttcac tttgtggtgc ttcttatttg tgccaccttg
     2701 tttacagtgg gaattagtat gttcatctac gatttcataa aatacggccg acctacagac
     2761 gaagctttgg atgaaaacca ataaaacaga cattaattaa ttcaataatt gcccgccaga
     2821 atcctttaat ttcccttttt agaaatgcat ttccatagaa aagacggtta atgggttctg
     2881 gtttttttaa aataaaataa aatgttagaa aacacacctt attatcacgc tgtgggaaaa
     2941 gaaattgaag tctttaacca agcgtataaa aacaaaatcc cttttctgtt aaaaggccca
     3001 acaggtactg gaaaatctcg ttttgttgaa tatatggcgc accaattgaa taaaaatatt
     3061 atcaccatta gctgtcacga agaaacgtca tctaccgatt tgattgggcg attcatcatc
     3121 aaaggtgctg aaacgatttg gctcgacggg ccattgacaa ctgcggtaaa agaaggtgcc
     3181 attatttatc tggatgaagt agccgaagcc cgtcccgatg taattgttgc cattcactcc
     3241 ttgacggatc acagacgcga actttttatc gacaaattag gcgaaacggt aaaagcccac
     3301 gacgatttta tgttggttgc ttcctttaat ccgggctacc aaagaggttt taaggaattg
     3361 aaaccttcaa cgcgtcaacg atttattgcg gtttcttttg attatccgga accaaaaatt
     3421 gagattgaaa ttttgattaa tgaaaccaat attgaccaag acaccgccaa aaaactagtt
     3481 gccattggta acaaaatcag aaacttgacc gaattagggt tgaccgaaac ggtttcgaca
     3541 cgacttttgg tcgatgctgc gaaaataatt cacagcggat tacccaaaag attgtccgtt
     3601 cacgtcgcca tcgtagaacc tttgactgat gatttgcaaa ccgtggaagc actgaaagat
     3661 ttgtgtaatt tgatgatata aaaaagtggt cattggtcag taacagagca ctgattactg
     3721 aacactgaat actgaacact aaaaaaatgg gtttcgaaat agatgaatat atagttggaa
     3781 aatttttcaa gcatttaaaa aggataaaaa aagtaaatcc tgaaataagt gccagaacta
     3841 tttctttgaa tgacatcaag ccacgattga ctattttggc acgagcattt actggaaagc
     3901 caatcgaaat tttcccagcg gaacgagaag gaggttataa aaacaataat ttttttcttc
     3961 ccatttcctt tgccgaattt tcaactattg aagaaaatcg aactttttac ctttttcgaa
     4021 ttttatattt aactattcaa caacgtttgg atttgaattg gaaagctgaa gaaaatgaac
     4081 caacattatc tctttcgcag caaaaagccg aagaaacatc ccatcaaatt ctgtctgttt
     4141 tattcgaaga atttccgatt acaaaagaaa tttacaacaa agccaaacgg cattttgaac
     4201 aaaaaattga ttcaaaaaca ggtgttgatt attcctggat ttacggaaaa tggatgcgaa
     4261 acgaagcaga acaaaaaaca gattcggttc ttgaaaattt ttcggataaa accaaaaaac
     4321 ccaacgaaaa ccaagcttta accacgttga aagcaaaagc cgtggaagaa gtgattaccg
     4381 ttgaattaga caaaaaacaa caggaagatt atgtgttgtt gcacaatttc gaaaaggcag
     4441 aaaccgccga agaattcaac ggaacttggc gcgattttga tggttcggac gaactcgaag
     4501 accatcagga agccttggaa gaattgaata tgaaatacac tgttcgggtc gatgatactt
     4561 cgcattcggt gtatcaagct gattttatcg aaaatacc
//
LOCUS       DAOSNN010000214         4598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_18810_length_4598_cov_4.065155, whole genome shotgun sequence.
ACCESSION   DAOSNN010000214 DAOSNN010000000
VERSION     DAOSNN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18810_length_4598_cov_4.065155"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PLQ70_10100"
     CDS             <1..853
                     /locus_tag="PLQ70_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQE35007.1"
                     /translation="SKKINICKLPDIELFKRLKVNEVPHEIIDWAGLSIFMRLQTLAT
                     RTAEMYIALGSDVHETAFTPTTYNGDYTVWLKNRLTYQFQNRLNILENNLHKLDGLAL
                     ELANQFLDHKKEIRKIFLDFDWTKMKSERIRIHGDYHLGQVLVNGDDFYILDFEGEPE
                     STIRDRKVKQPPLKDVAGMFRSFHYAIYATIFNNKDKYPFDQEELFKAGEILFKYFVG
                     VFIQTYTDVAQGENLNIGYRKEIDFLLKYCLLEKAVYELGYELNSRPRWAVIPLTGIA
                     SIMEFKN"
     gene            898..2799
                     /gene="glgB"
                     /locus_tag="PLQ70_10105"
     CDS             898..2799
                     /gene="glgB"
                     /locus_tag="PLQ70_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HQE35008.1"
                     /translation="MNKVQSYSLFTDFDIDLFKAGKHFRLYEKLGAHLVEVDGVKGVY
                     FAVWAPSARSVSVVGDFNYWIQGDHLLQVRWDSSGIWEGFIPGIEKGTTYKYKIQSNN
                     GGIITEKADPFALYCEHPPHTASVVWDLDYQWKDKKWMETRKEHNDLDKPYSVYEVHL
                     GSWKRNSEGKFLTYLELADDLVAYIKETGFTHVEFMPIMEYPYDPSWGYQLVGYFAPT
                     SRFGTPQDFMVLVDKLHQAGIGVILDWVPSHFPEDAHGLGFFDGSNLFEHPDRRKGYH
                     PDWKSLVFNYGRNEVRAFLISNAIFWLQHYHVDGLRVDAVASMLYLDYSRNEGEWEPN
                     IFGGRENLDTISFLKEFNEAVYSSFEGVQTIAEESTSFPMVSRPTFVGGLGFGMKWMM
                     GWMHDTLQYFQKETVYRKYHQNDLTFSMTYAFSENFMLPLSHDEVVYGKKSIAGRMPG
                     DEWQKFANLRLLYGYMFMHPGAKLLFMGSEFGQSAEWNFEGSLDWHLLQYDFHEGVKK
                     TITDLNALYKTQPALHEKQFSPEGFEWINYSDHQNAVMTFIRKGNNSKDDVIVVCNFT
                     QVVRENYRIGVPKKGKLTEIFNSDSSVYGGSGVKNTKKLTIEALPYDGRDYSVELLLP
                     PLSVTVYKY"
     gene            2852..3670
                     /locus_tag="PLQ70_10110"
     CDS             2852..3670
                     /locus_tag="PLQ70_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQE35009.1"
                     /translation="MKKHNLLVGICALGLFFSCATNPFTGKQSLNFVSNSELFPSSFQ
                     QYGTFLKENKVIAGTTDAIRVETVGMKIKAAAERWLKANGQTNYLNGYQWEYKLIENK
                     EVNAWCMPGGKIVVYSGILPVSQDDAGLATVMGHEVSHALANHGAQRMSAAQLQQLGA
                     VGVAIATGNKSAEQQQMWQQYYGLGSEVGVMLPFSRNHESEADKIGLTLMAIAGYNPE
                     LAIKFWERMSASSSGNKPPEFLSTHPADATRIANLRRLIPEAKAEALKFGVVFN"
     gene            complement(3738..>4598)
                     /locus_tag="PLQ70_10115"
     CDS             complement(3738..>4598)
                     /locus_tag="PLQ70_10115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HQE35010.1"
                     /translation="EVTDNNGNVTKSKIQHVWVGKNLDVAPKIAFEPVSNYSATPIDV
                     KITATDSNDPSPKVYYTTDGSTPTTLSPTFVSTKTINITTTTTFNILAVDADGNQSTA
                     TQKFSIGNISPITVYFKPLASWGIPKIYYWSALPSGAVPAITWAQSIAMTADTNGWYK
                     YTFDGVTSVNVIFRNQAGNIQSLDLTGITNDKWFDSSYNSVLSVYSKEISTNDFILYP
                     NPTYGTLKVESREKISEAGVFDLKGTLVSFQSIGNNQLIELGNLISGTYIIKMITEKR
                     QTIYKKIIKK"
BASE COUNT         1488 a          694 c          920 g         1496 t
ORIGIN      
        1 gtctaaaaaa attaatatct gcaaattacc tgatattgaa ttgtttaaaa gattaaaagt
       61 caacgaagtt ccacacgaaa tcatcgattg ggccggattg agtatattta tgcgacttca
      121 aactttggca acacgaactg ccgaaatgta tattgcatta ggaagtgatg ttcacgaaac
      181 cgcttttact ccaacgactt ataatggaga ttacaccgtt tggcttaaaa acagattgac
      241 ctatcagttt caaaatcggt tgaatattct ggaaaataac cttcataaac tggatggttt
      301 ggctttagaa ttagccaatc agtttttgga tcataaaaag gaaattagaa aaatatttct
      361 agatttcgat tggacaaaaa tgaaatctga aagaattcgc attcatggcg attatcattt
      421 aggtcaggtt ttggtcaatg gtgatgattt ttatatttta gattttgaag gagaacctga
      481 aagtactatt cgggatcgaa aagtaaaaca accaccattg aaagatgttg caggaatgtt
      541 tcgttcgttt cattatgcta tttacgctac gattttcaac aacaaagaca aatacccttt
      601 tgaccaagag gaacttttca aagctggaga aattttgttt aaatattttg taggtgtctt
      661 tattcagact tatactgatg tagctcaagg cgaaaacttg aatattggct accgaaaaga
      721 aatagatttc ttattgaaat attgtttgct tgaaaaagca gtttatgagt tgggatatga
      781 actcaattct cgtccgcgtt gggctgtaat tcctctgact ggaattgcta gtattatgga
      841 gtttaaaaat taaataactg gttaaccata ttggttaaat aaaagaaaaa aaataaaatg
      901 aacaaagtac aatcctattc cctttttacc gattttgata ttgatttatt caaagcggga
      961 aaacattttc gattatatga aaaactaggc gcacatcttg tagaagttga cggagttaaa
     1021 ggtgtatatt ttgctgtttg ggcaccatca gcgcgatctg tttctgtagt tggggatttc
     1081 aattattgga ttcaaggcga tcatttattg caagtgcgtt gggattcctc tggaatttgg
     1141 gaaggattta ttccgggaat cgaaaaagga acaacatata aatataaaat tcaatccaac
     1201 aatggtggaa ttataactga aaaagccgac ccatttgcgc tgtattgcga gcatcctcct
     1261 catactgcct cagtagtttg ggatttagat taccaatgga aggacaaaaa gtggatggaa
     1321 acccgcaagg aacacaatga tttagacaaa ccttattcgg tttatgaagt tcatttgggg
     1381 tcttggaagc gcaattcaga aggaaaattt ttgacatatc tcgaattggc agatgattta
     1441 gttgcatata ttaaagaaac aggttttact cacgttgagt ttatgcccat aatggaatat
     1501 ccatatgatc cttcttgggg ctatcaattg gtggggtatt ttgcacccac ttctcgtttt
     1561 ggaacacctc aggattttat ggttttggtc gataaattgc accaagcagg aattggagta
     1621 attttggatt gggttccatc ccattttcca gaagacgctc acggtttagg cttttttgat
     1681 ggctcgaatt tatttgaaca tcctgaccgc cgaaaaggct atcatcctga ttggaaaagt
     1741 ttagttttca attatggacg aaatgaagtg cgagcgtttt tgataagtaa tgcgattttt
     1801 tggttgcaac actatcacgt cgatggactt cgggttgatg ctgtagcttc gatgttgtat
     1861 ttggattatt ccagaaatga aggggaatgg gaacctaata tttttggcgg aagagaaaat
     1921 ctggacacca taagtttcct aaaagaattt aatgaagccg tttattcaag ttttgaaggc
     1981 gtacaaacta ttgctgaaga aagtacttct ttcccgatgg tttctcgtcc aacatttgta
     2041 ggaggattag gttttggaat gaaatggatg atgggttgga tgcacgatac tttgcagtat
     2101 ttccaaaaag aaacggtgta tcgaaaatac catcagaatg atttaacttt ttcgatgact
     2161 tatgcttttt ctgaaaattt tatgctacca ctttctcacg acgaagtagt ttatggtaaa
     2221 aaatctatcg ctggaagaat gcccggtgac gaatggcaga aatttgctaa tttaagattg
     2281 ctttatggct atatgtttat gcacccggga gcaaaattat tgtttatggg aagtgaattt
     2341 ggacaaagtg ctgaatggaa ttttgaaggt agtttagact ggcatttgtt acaatatgat
     2401 tttcacgaag gtgtcaaaaa aacaataacc gatctgaatg ctttgtataa aacacagcca
     2461 gcattgcacg aaaaacaatt tagtccagaa ggtttcgaat ggatcaatta ttcagaccat
     2521 caaaacgccg tgatgacttt tattcgaaaa gggaacaact ctaaagacga tgtaattgtg
     2581 gtttgtaatt ttacccaagt agttcgcgaa aattatcgta ttggtgtacc caaaaaagga
     2641 aagttaacag aaattttcaa tagtgattcc agtgtttatg gtggaagtgg tgtaaaaaat
     2701 acaaaaaaat tgaccatcga agcgttgcca tatgatggca gagattattc ggttgaattg
     2761 ttattgccac cattgagtgt gacggtttat aaatattaga gccaaatttt tctcgagtaa
     2821 ttatccaaaa atattgatac ttttattttt tatgaaaaag cataatttat tagttggaat
     2881 ttgcgcatta ggattatttt tttcttgtgc cacaaatcca ttcaccggga aacaaagttt
     2941 aaattttgta tcaaatagtg aattatttcc ttcttctttt caacaatatg ggactttttt
     3001 gaaagagaat aaagtcattg caggcaccac ggatgcgata cgagtagaaa ctgttggtat
     3061 gaaaatcaaa gcagctgcag aacgctggtt aaaagctaat ggacaaacca actatttaaa
     3121 tggttatcaa tgggaatata agttaataga aaataaagaa gttaatgctt ggtgtatgcc
     3181 aggtggtaaa attgttgtgt actcgggaat tttaccagta tctcaagatg atgcagggtt
     3241 agctactgtt atgggacatg aagtttcgca tgccttggcc aatcatggtg ctcaacgaat
     3301 gagtgctgca caattgcagc aacttggagc agtaggtgtt gcgatagcta caggaaataa
     3361 aagtgccgag cagcaacaaa tgtggcaaca atattacgga cttggttctg aagtaggagt
     3421 gatgcttcct tttagtagaa atcacgaaag tgaagccgat aaaatcggtt taactcttat
     3481 ggcgattgca ggatataatc ctgaattagc aataaaattt tgggaaagaa tgtcagccag
     3541 tagtagcggc aataaaccac ctgaattttt gagtacacat ccagccgatg ctacaagaat
     3601 tgctaattta agaaggctaa ttccagaagc taaagccgaa gctttaaaat ttggagttgt
     3661 ttttaattaa ttgaacttat gttaaaaaag gaagtaatct aaaaacaaga ttacttcctt
     3721 ttttttatga aaactattta ttttttgata atctttttgt aaatagtttg tcttttttca
     3781 gttatcattt ttatgatata agtacctgaa attaaattac ccaattctat aagctgattg
     3841 tttccaatac tttgaaatga aaccaaagtt ccttttaaat caaatactcc agcttcacta
     3901 attttttctc ttgattcaac ttttagagtt ccgtaagttg gatttggata tagaataaaa
     3961 tcattagttg aaatttcttt actataaaca gaaagaacag aattataact agaatcgaac
     4021 catttgtcgt tggtaattcc tgtcaaatca aggctttgaa tgttacccgc ttgatttctg
     4081 aaaattacat ttactgaagt aacgccatcg aaagtatatt tataccaacc atttgtatct
     4141 gctgtcatcg caatagattg tgcccaagta attgcaggaa ctgcaccaga aggcaatgcc
     4201 gaccaataat agatttttgg aattccccaa cttgccaaag gtttaaaata aacagtaata
     4261 gggcttatgt ttcctataga aaacttctgt gtggctgtac tttggtttcc atcagcatca
     4321 actgctaaaa tattgaaagt tgtagtcgtt gtaatgttga tggttttggt cgaaacaaaa
     4381 gttggagaca aagtagttgg tgtacttcca tcagtggtat aatatacttt tgggcttgga
     4441 tcatttgaat ctgttgcagt aatttttaca tcgataggag ttgcagaata attggataca
     4501 ggttcgaaag caatttttgg tgcaacatct aaattttttc ctacccaaac atgctgaatt
     4561 tttgattttg taacatttcc attattgtcg gtaacttc
//
LOCUS       DAOSNN010000215         4584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_18897_length_4584_cov_4.722014, whole genome shotgun sequence.
ACCESSION   DAOSNN010000215 DAOSNN010000000
VERSION     DAOSNN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18897_length_4584_cov_4.722014"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLQ70_10120"
     CDS             <1..430
                     /locus_tag="PLQ70_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQE35011.1"
                     /translation="LSKKFIIRPNFTLSDNKNVDMNIGLGDVTTNIAYSPSVIFGNIL
                     VYSPIEQLHISLLQKFVGDQYMNNFDSVESRLPDYFVNDLNIVYSIKPKTIFKEIVLT
                     GLVNNMLNKKYVSNGYMWDVYPYYYPQSGINFLAGMTLKF"
     gene            complement(492..941)
                     /locus_tag="PLQ70_10125"
     CDS             complement(492..941)
                     /locus_tag="PLQ70_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35012.1"
                     /translation="MKKYLLLLIAFPLLFGCDTNSTNNNNPYIPNYSFTLDINMSLPA
                     YSSLKFVSNAVYYPSQGARGLYIFNTGSGYVAFDGACPNQALTSCSTMNFKKLDPNDP
                     LKIDKTTIVCPCDNAEYSLFSGQSAGKQYPLKQYRVEVNGEILRIYN"
     gene            1108..1584
                     /gene="greA"
                     /locus_tag="PLQ70_10130"
     CDS             1108..1584
                     /gene="greA"
                     /locus_tag="PLQ70_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQE35013.1"
                     /translation="MTKVSYYTAEGLKKLRDELDYLKSVMRPKASQDIADARDKGDLS
                     ENAEYDAAKEAQGMLEMRIAKLEEVHSNARLIDETQLDMSKVLVLSNVKIKNQTNGME
                     MKYTLVAESEADLKTGKISVTSPIGRGLLGKSVGEVAEITVPNGVLKFEILEVSRD"
     gene            1684..2070
                     /locus_tag="PLQ70_10135"
     CDS             1684..2070
                     /locus_tag="PLQ70_10135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HQE35014.1"
                     /translation="MTVFTKIINGEIPCYKIAEDENFFAFLDINPNAKGHTLCVPKQE
                     IDKLFDLDDTTYLGLMQFSKKIAAALKKTVPCDRIGMTVIGLEVPHAHVHLIPLNEMR
                     EMTFKHKVNLTKEEFEVLAKEIQANL"
     gene            complement(2118..3263)
                     /locus_tag="PLQ70_10140"
     CDS             complement(2118..3263)
                     /locus_tag="PLQ70_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQE35015.1"
                     /translation="MQFSKSRNTSRWIIIFASFFIISLILWNTYTFFQIFKHEERLKM
                     SLWANAEKILINAGENTDLELPLQIFSNNTSIPIMLTENDSIINSKNIDEKIIEDKKR
                     ANAFLSGLKNENDPIIIEYVPGKFQKIYYGDSELLKKLKYYPIALLLIIILFAALVYN
                     FYKSTKMATQNKLWAGMAKETAHQIGTPLTSLVGWVEILKSENIDKSTTDEIEKDIER
                     LQTITERFSKIGSEPKLENRDIVEETHQSYKYLQSRFSKQVEFSFKGPDNPIFASFNP
                     TLHGWTIENLVKNAIDAMKGRGKLSVSVEEEGEFVKIKVSDTGSGIPKNQYKSVFEPG
                     FTTKKRGWGLGLSLTKRIVEEYHKGTIKVLHSEIGKGTTMQVSILKN"
     gene            3450..3827
                     /locus_tag="PLQ70_10145"
     CDS             3450..3827
                     /locus_tag="PLQ70_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HQE35016.1"
                     /translation="MEVIGKIKMIDQTKEVGSSGFKKRDVVVTTDEQYPQHILVQFVQ
                     DKCDLLNNYQVGEAVKIDINLRGREWTNPQGETVYFNTIQGWRIAKAQAEAPAAPAPP
                     MPAAAAFPPATSINEDEPDDLPF"
     gene            complement(3883..4584)
                     /locus_tag="PLQ70_10150"
     CDS             complement(3883..4584)
                     /locus_tag="PLQ70_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQE35017.1"
                     /translation="MVFIGNAQTKDSISSLKPKEKITYKQLIAPAALITTGTLLLNTK
                     LNDDLQKNANKLFGDNFHTSADNYLQFVPVAQIYLGKTFGFQPKNNFKQQTINIVVAN
                     LIMGTTVFALKNTTNQERPDQSDNLSFPSGHTALAFTNASLLYYEYKDSNLWYASSGF
                     LFATATGVLRIANNKHYTSDVLAGAGIGLASGLIVSYWNPFHSMTLGKKKKTTAFVYP
                     QIGSQIGMGALIQLN"
BASE COUNT         1531 a          694 c          836 g         1523 t
ORIGIN      
        1 attgtctaaa aagtttatta ttcgacctaa ttttactttg agtgataata aaaatgtcga
       61 tatgaatatt gggttaggag atgtaaccac taatattgct tattcacctt cggtaatttt
      121 cggaaatatt ctggtgtatt cgcccatcga gcaattgcat atttcgttgt tgcaaaagtt
      181 tgtaggagat cagtatatga ataattttga ttcagtagaa tccagattgc ctgattattt
      241 tgtgaacgat cttaatattg tatattccat aaaacctaaa accattttca aagaaatagt
      301 tttaacaggt ctggtaaata atatgttgaa caagaaatat gtgtcaaatg gttatatgtg
      361 ggatgtgtat ccttattatt atcctcagtc aggaattaat ttcttggcag gaatgacttt
      421 gaagttttag attatttttg ttatactttg tagaatcctg acagtttttt tagacggtca
      481 ggattttttt gctaattgta aatgcgtaat atttctccgt taacttccac gcgatattgc
      541 ttcaatgggt attgtttgcc agcactttga cctgaaaaca aactgtattc tgcgttgtcg
      601 caagggcaaa caattgttgt tttgtcaatt tttaatgggt cattagggtc taattttttg
      661 aaattcattg tagaacaact tgtcaatgct tggttaggac aagcaccatc aaaggcaaca
      721 taaccacttc cagtattgaa aatatacaaa cctctagcgc cttgacttgg gtaataaaca
      781 gcattactca cgaattttag actggaataa gcaggcaaac tcatgttaat atcaagagta
      841 aaagagtaat tcgggatgta aggattgttg ttattcgtag aattcgtgtc gcaaccaaaa
      901 agcaatggaa aggcaatcaa gagaagaaga tattttttca ttattcagaa tataattgat
      961 ttattagtca gacaaattta atttatttag tttttaaatg gatatgttta acttatattt
     1021 atagattaaa ataaaaatag tactatcttt gtgaaaagaa atcccgtcct gatgggattt
     1081 tttctatttt atataaatga tgaagttatg acgaaagtat cttattacac agccgaagga
     1141 ttaaaaaaat taagagacga actagactat ttaaaaagcg tgatgcgtcc aaaagcatct
     1201 caagatatag cagatgcaag agataaaggc gatttatccg aaaatgccga atacgatgct
     1261 gccaaagagg cacaaggaat gttggaaatg cgaattgcta agctagaaga agtacattcg
     1321 aatgcgagat taattgacga aactcaatta gatatgtcaa aagttttggt attatcgaac
     1381 gtgaaaatca agaaccaaac caatggaatg gaaatgaaat acacacttgt ggccgaaagt
     1441 gaagctgatt tgaagaccgg aaaaatctct gtgacttcac caattggtcg aggtttgttg
     1501 ggaaaatctg ttggagaagt tgctgaaatc acagtcccaa acggtgttct gaaatttgaa
     1561 atattagaag tttcgagaga ctaatttttt gtttaagagt ttaaaaattg tttgaaggtt
     1621 taagttgttt ttagaagttt aaacctttaa actattttaa acaaaatagt aaatttatta
     1681 aaaatgacag tttttactaa aatcataaac ggagaaattc cttgctacaa aatagccgag
     1741 gacgaaaatt tctttgcttt tttggatatt aatccaaatg caaaaggaca tactttatgt
     1801 gttccaaaac aagaaataga taaattattc gatctcgatg acactactta tcttgggcta
     1861 atgcagttct ccaaaaaaat tgcagcagca ctcaaaaaaa cagttccttg cgatagaatt
     1921 ggaatgactg tcataggact tgaagtgcca cacgctcacg ttcatttgat tcctttgaac
     1981 gaaatgcgag aaatgacatt caaacataaa gtgaatttga ctaaagaaga atttgaagtt
     2041 ttggctaagg aaattcaggc taatttgtaa gaaaagtgag ttataaaata aaaaacacta
     2101 aactaaaaca cgacaaatca attttttaga atactcactt gcattgtcgt tccttttcca
     2161 atttcagaat gcaagacttt tattgttcct ttatggtact cttcgacaat tcttttggtt
     2221 agcgaaagtc ctaaacccca acctcttttt ttggtcgtaa aaccaggttc gaagacgctt
     2281 ttgtattggt tttttggaat tccacttcct gtatccgata cttttatttt tacaaattcg
     2341 ccctcttctt caacagaaac agataacttt ccgcgacctt tcatggcgtc aatagcattt
     2401 tttacaagat tttctattgt ccagccgtgt aaagtgggat tgaaagaagc aaatattgga
     2461 ttatcaggac ctttgaatga gaactccact tgttttgaaa atcgggattg caaatattta
     2521 tacgattgat gggtttcttc aacaatatct ctgttttcga gtttaggttc tgagccaatt
     2581 ttcgaaaaac gttcggtgat ggtttgcaag cgttcaatat ctttctcgat ttcatcggtt
     2641 gtcgatttat cgatattttc agatttaaga atttcgaccc agcctactaa tgatgtcaat
     2701 ggtgtgccaa tttgatgcgc agtttctttg gccattcctg cccaaagctt gttttgagtt
     2761 gccattttgg tactcttata aaaattataa accaatgctg caaacaagat aatgatgagt
     2821 aataaagcaa taggataata tttgagtttt ttcaataact ctgaatctcc ataatatatt
     2881 ttttgaaatt ttccgggcac atattcaata atgatggggt cgttttcatt ttttagtcca
     2941 gacaaaaagg catttgctct ttttttatcc tcaataattt tctcgtctat atttttagaa
     3001 ttgatgatgc tgtcgttctc cgttagcata atcggaatag aagtattgtt gctgaaaatt
     3061 tgaaggggaa gttctaagtc agtgttttct ccagcattga ttagaatttt ttcagcattg
     3121 gcccaaagac tcattttcaa tcgttcttcg tgtttgaaaa tctgaaaaaa agtataggtg
     3181 ttccaaagta ttaatgaaat aataaaaaag gaagcaaaaa taataatcca acgactggtg
     3241 tttctacttt tggaaaactg cataaattaa tttttgaggc ataaatataa cgaaataatt
     3301 taagtatgat tgtgtttatt tgggagttat tctttttaga gaatatccga aaaaaatcga
     3361 attaaaatat tttctgaaaa ttgtatttaa tttgaaacta actgataaaa tactatattt
     3421 gtgtatcaca aaaaaaataa aaaattaaga tggaagttat agggaaaatc aaaatgattg
     3481 atcaaaccaa agaggttggg tcatcgggtt tcaaaaaaag agatgttgtt gttacaactg
     3541 acgaacaata tccacaacat attttggttc agtttgttca agacaaatgt gatttgttga
     3601 ataattatca agtaggcgaa gctgtaaaaa ttgacattaa tttaagaggt cgtgaatgga
     3661 ctaatccaca aggagaaacc gtttatttta atactattca aggatggaga atagcaaaag
     3721 cacaagcaga agctcctgcg gctccagcgc caccaatgcc tgccgctgca gcatttccgc
     3781 cagcaacaag catcaacgaa gatgaaccag acgatttacc gttctaaata aaactaaaaa
     3841 atccttgaaa ttttcaagga ttttttttgg tctttgacga aattaattca actgaattaa
     3901 tgctcccatc ccgatttgac ttccaatttg gggataaaca aaggccgttg tttttttctt
     3961 tttacccaaa gtcatcgaat gaaacggatt ccaataggaa acaatcaatc ctgatgctaa
     4021 tccgattcca gctccggcaa gaacatccga agtataatgt ttgttgttag caattctaag
     4081 aactccagtt gctgtggcaa acaaaaatcc gctactagca taccacaaat ttgaatcttt
     4141 gtattcgtaa tataataaag aagcgttggt aaaagctagt gccgtgtggc ctgaaggaaa
     4201 acttaaatta tcggattgat cgggtctttc ttggttggta gtatttttta gggcgaaaac
     4261 agtggttccc ataatcaaat tggcaacgac tatattaatg gtttgctgtt tgaaattgtt
     4321 tttgggttga aatccaaagg tctttcctaa ataaatttga gcaacaggaa caaattgaag
     4381 ataattatca gccgaagtat gaaaattgtc gccaaacaat ttgttagcat tcttttgtaa
     4441 atcatcattc agtttagtgt tcaaaagcaa ggtccctgtg gttatcaggg cagcgggagc
     4501 aataagttgt ttgtaagtta ttttttcttt gggttttagg cttgaaatag aatctttggt
     4561 ttgcgcattt cctatgaaaa ccaa
//
LOCUS       DAOSNN010000216         4581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_18911_length_4581_cov_6.076668, whole genome shotgun sequence.
ACCESSION   DAOSNN010000216 DAOSNN010000000
VERSION     DAOSNN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18911_length_4581_cov_6.076668"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..544)
                     /locus_tag="PLQ70_10155"
     CDS             complement(236..544)
                     /locus_tag="PLQ70_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35018.1"
                     /translation="MKSTREIFKTNPSLLDEPEVAKLIAYCEELQDEIVEFKFQKTDN
                     KELAMLDMLKEVIKGCNAIEKEQMEHERFGFEAPDYQSTISNLKSYILKRCQDEKIYL
                     "
     gene            1014..3797
                     /locus_tag="PLQ70_10160"
     CDS             1014..3797
                     /locus_tag="PLQ70_10160"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQE35019.1"
                     /translation="MPKTSLLIAILSLFSLSVFAQKASVTGVLTDGNEKTPVYNSVVA
                     LLTPKDSILYKFTRSDKKGNFELKNVKAGNYIVMTSHRQYADYLDDITVNETEKNLGT
                     IALVSKINALREVIIKTGSIRIKGDTTSYRASDFVVSANANVEELLKKLPGIQVDKNG
                     TIKAMGETVQKVLVDGEEFFGDDPGMAVKNLRADAIKEVQVFDKKSDQAEFTGIDDGE
                     KKKTINLKLKEDKKKGYFGKIDGANEPFTGSDSRYNTNLMFSSFKGKRKISAFLLNGN
                     TGQDGLSWQDSDKFGGRDSNVSMSMDDDGNVNYEWSGGNNDEEPYVDTQNGFVKNTNA
                     GLQYSNKWNDKQTLNLTPKYNKQIYTNNNNKKTQTQVGETELIENTSKVSNVDRSNFK
                     LNAIYDVKIDSLNSLQITTKTNFYNTESGEFTNGNTTGIDGLLKNKQLKTFTTKSDKQ
                     SLFASVLYKHKFAKPRRTFSVNASASTLNTDANNFLKSSNESYEDGNFASRVDLDQNK
                     IEEKKNQNLTLNLTYSEPIGKKYAMQFAYQVTQNKGDSNYLTYNYSDLTGNYDELVNS
                     LSNQFYQTIITNKPNIRLSYNSKKINYNLGGGIGFTSFDLEDQTMDKEYKRNYINFFP
                     SANFTFKYKSNSNLRINYQGATKQPTLDQLQPLRDNQDFFNQTLGNPDLKQSFTNSFN
                     LFNSTYSILTETQFYQSFSYRTTSNLISYSRVIDPESALTITKPINTNGNYSLNLYVG
                     YGFKVKKLDLWVNLNPNLNYNKSVNSINLRTNDTKTLNSGFSVYLNKSKEKKYDITLS
                     NNFSNSRNTTSQSDEIKSFNTNNLALDIGVYFKEKWKLSTDYNLYSRQKTVDFQDNLT
                     NQLWNARLQRTFKNDEFTAYIMVRDILNQNIGIRRYVYENSITEEQNDRLKRYAMIGF
                     TWNFKNKGEKTNK"
     gene            3848..>4581
                     /locus_tag="PLQ70_10165"
     CDS             3848..>4581
                     /locus_tag="PLQ70_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HQE35020.1"
                     /translation="MKSILFILTTLLFATTATAQQFIEKAVVEYEVNTNLKKTMSNDS
                     WDEMMKDNLSDLKISYYNYTFADNKSIFKFDRWSPKTRIPKYQKDLDEENTWYFDFGA
                     NKMMMQKQIVGTNFVISDSIQNIEWKITNENREIAGYNCRKAVGKIMNDVYVFAFYTD
                     EITITGGPCSIHGLPGLILGLSIPRLYTSYVATKVDLTMTKASEIKPITAKKTYDLLG
                     LKKEMEEKTKEWFNYGDDKEENLRQK"
BASE COUNT         1778 a          689 c          752 g         1362 t
ORIGIN      
        1 acaattcatt gctttggaat agcagtaatt ccaattgtga tttaaaatcg ggtgtctata
       61 tcatttcttt ttctaacacc atttcaacta ttaaaaaaga agtgaaacta atcaaaaaat
      121 aagattgaca tcgatgattg gataaaaagg aaatcaataa taacaagttg taagggttga
      181 aggtgtaaaa acattcaacc ctttttgata gttgtaaatt tgttaatgga atatattaca
      241 aatagatttt ctcgtcttgg caacgcttta aaatatagct ttttagattt gaaatggtgc
      301 tttgataatc aggtgcttca aaaccaaaac gctcgtgttc catttgttct ttttcgatgg
      361 cgttgcaacc tttgataact tccttgagca tatcgagcat tgccaattcc ttgttgtccg
      421 ttttctgaaa tttaaattcc acgatttcgt cttgcaattc ttcgcaataa gcaataagtt
      481 ttgctacttc gggttcatcc aaaagggaag ggttggtttt gaatatttct cgggtggatt
      541 tcataattaa attataattt aaaaaaattc ggttttacag gaaatgtagt gagaattaca
      601 aacacattac tactaaaatc cgaaaaaaaa acaaatttca catttcattt ttaaccgcaa
      661 aaatcagaaa ggtatcgcaa gcaaaggttg caaaaaatag taaagacaca agacgcttcg
      721 tttgacttga aattatattt tcaggtaaaa acgagttgag ttaaaaaaat ttaaattatt
      781 gaaataaaac gaattataat tttctgtcga agcatttgtt ttcattcata cgaatagtat
      841 tatgaattaa ttaacagctt gttttaaata atttaggaat attaatacta tttttgattt
      901 ttttgagttc tattttctaa ttcaaaacta actttatcga aagagtttct gctttttgga
      961 taccttaatt ttataaattt ttaaagaata ataaagtaaa aataaaacta tttatgccaa
     1021 aaacttcact tttaattgca attttatctt tattttcttt atctgttttt gctcaaaaag
     1081 ccagtgttac gggggttttg actgatggta acgaaaaaac tccagtgtac aattcggtgg
     1141 ttgccttact aacacccaaa gattctattt tatataagtt tacccgatcg gataaaaagg
     1201 ggaactttga gcttaaaaat gtaaaagcag gaaactatat tgtgatgact tctcacagac
     1261 aatatgccga ttatttagat gatatcactg taaatgaaac tgaaaaaaat ttaggaacaa
     1321 tagctttggt aagcaaaatt aatgcactta gagaagtaat tataaaaaca ggttccataa
     1381 gaattaaagg cgatacgact agttacagag ccagtgattt tgttgttagt gccaatgcta
     1441 atgtagaaga attactaaaa aaacttccag gaatacaagt cgataaaaat ggtacaataa
     1501 aagctatggg cgaaacggta caaaaagtat tggttgatgg cgaagaattt tttggagatg
     1561 acccaggaat ggccgtgaaa aatttgcgag cagatgccat aaaagaagtg caggtttttg
     1621 ataagaaaag tgaccaagcc gagtttacag gaattgatga tggtgaaaaa aagaaaacga
     1681 ttaatctaaa attaaaagaa gacaaaaaga agggatattt tggaaaaata gatggtgcca
     1741 atgagccatt tacaggttcc gattccagat acaataccaa tcttatgttt agtagtttta
     1801 aaggcaaacg aaaaatatcg gcgtttttat tgaatggcaa taccggtcaa gatggattga
     1861 gttggcaaga tagcgataaa tttggaggac gtgatagtaa cgtgagtatg agtatggatg
     1921 atgatggcaa cgtaaattat gaatggtcgg gcggcaataa tgatgaagaa ccatatgttg
     1981 atactcaaaa tggatttgtc aaaaacacca acgcaggatt gcaatacagc aacaagtgga
     2041 atgataaaca aacattgaat ttaacaccaa aatacaacaa gcaaatttat accaacaaca
     2101 ataacaaaaa aacacaaacc caagtaggag aaaccgaatt aatcgaaaac acgtctaaag
     2161 taagcaatgt tgataggagt aattttaaat tgaatgctat ttacgatgta aaaatagatt
     2221 ctttaaacag tttacaaatt accaccaaaa ctaattttta taatacggaa agtggtgaat
     2281 ttaccaacgg aaacactacc ggaatagatg gattgttaaa aaacaaacag ctaaaaacat
     2341 ttacaacaaa atcagataag caatccttgt ttgccagtgt tttatataaa cataaatttg
     2401 ccaaacctcg tcgtacattt tctgtaaacg ctagtgcgag caccttgaat acagacgcaa
     2461 ataatttttt aaagtcttcg aacgaaagtt atgaagacgg taattttgct tctagggtcg
     2521 atctcgatca aaacaaaatt gaggagaaaa aaaatcaaaa tttaacttta aatcttactt
     2581 attcagaacc tattggtaag aaatatgcaa tgcaatttgc ctatcaagtt actcaaaaca
     2641 agggagacag caattatttg acttataatt attcggatct aacaggaaat tatgacgagc
     2701 tcgtcaattc tttgtcaaac cagttctacc agactataat aaccaacaag cccaacatta
     2761 gattgagtta taattccaaa aaaatcaatt acaacttagg aggtggtatt ggatttactt
     2821 cgtttgattt ggaagatcaa actatggaca aagaatacaa acgcaattat atcaattttt
     2881 ttccgtctgc aaattttact tttaaataca aaagcaatag caatttgcgt atcaattatc
     2941 aaggagctac aaaacagcca acactcgacc agttgcagcc tttgagagac aatcaagact
     3001 tttttaatca aaccttggga aatccagatt taaagcaatc ttttactaat agtttcaatt
     3061 tatttaacag cacttatagc attttgacag aaacgcaatt ctatcaaagt ttctcataca
     3121 gaacgacttc taatttaatt tcttatagca gagtcatcga tccagaaagt gccttaacca
     3181 ttaccaaacc catcaatacg aatggtaatt attcactcaa tttgtatgta ggatatggtt
     3241 ttaaggtaaa gaaactcgat ttatgggtta atcttaatcc aaatctaaac tacaataaat
     3301 cagtaaatag catcaacctt agaactaacg atactaaaac tttaaattca ggattttcgg
     3361 tttatttaaa taaatcaaaa gaaaagaaat atgacattac tttgagtaat aatttttcaa
     3421 atagccgaaa tacgacttca caaagcgatg aaataaaatc attcaatact aataatttag
     3481 ctttggatat tggggtttat ttcaaagaaa aatggaaact atctacagat tataatttgt
     3541 attctcgtca aaaaacagtc gattttcagg acaatctcac caatcaattg tggaatgcaa
     3601 gactacaacg cactttcaaa aacgatgaat ttaccgctta tataatggtg cgagacattt
     3661 tgaatcaaaa tattggcatc agaagatatg tttatgaaaa ctccattacc gaggagcaaa
     3721 atgacagact caaaagatat gctatgatag gatttacctg gaattttaaa aacaaaggcg
     3781 aaaaaacaaa taaataaatt cggtattgca ctaaaatcca aaacgaatta ccataaaaat
     3841 tacagctatg aaatcaatac tttttattct tacaacactc ctttttgcaa ctacagcaac
     3901 agcgcaacaa tttatagaaa aagcagtagt tgaatatgaa gtaaatacga atttaaaaaa
     3961 aacaatgagc aatgatagtt gggatgagat gatgaaagac aacctttctg acctgaaaat
     4021 atcgtattac aactatactt ttgcagacaa taaaagcatc ttcaaatttg accgatggag
     4081 tccaaaaaca agaattccta aataccaaaa agaccttgac gaagaaaata cttggtattt
     4141 tgattttggc gccaataaaa tgatgatgca aaaacaaata gtaggaacta attttgtcat
     4201 atcggacagc atccaaaata ttgaatggaa aattacaaat gaaaacagag aaatagcagg
     4261 ttataattgc cgaaaagccg ttggcaaaat aatgaatgat gtttatgtat ttgcttttta
     4321 tactgatgag atcactatta ctggagggcc ttgttccatc cacggattgc caggtttaat
     4381 tttggggctt tccattccca gattatacac ctcctatgta gccaccaaag tagatttgac
     4441 gatgactaaa gcttccgaaa taaaacccat cacagccaaa aaaacctatg atttattggg
     4501 tctaaaaaag gaaatggaag aaaaaacaaa agaatggttc aactatggag atgataaaga
     4561 ggagaactta agacaaaaaa a
//
LOCUS       DAOSNN010000217         4541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_19153_length_4541_cov_4.131297, whole genome shotgun sequence.
ACCESSION   DAOSNN010000217 DAOSNN010000000
VERSION     DAOSNN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19153_length_4541_cov_4.131297"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /locus_tag="PLQ70_10170"
     CDS             <1..1842
                     /locus_tag="PLQ70_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HQE35021.1"
                     /translation="MYSENPTGIDPYNYSTSVSSDKCGSYSVEGDCYNREHIIPQSVF
                     NSNSPMVADAHFITPTDGKVNGLRSNFPHGIVASASKTTLNGSKLGTSGVSGYSGTVF
                     EPIDEFKGDIARMYFYFATRYENNVAGYSYDMFNGTSGQVFTPEFLSMLLAWHAQDPV
                     NAREIARNNAIYARQNNRNPFIDHPEYVQLIWNPTADTVAPTAATNLTVTGTTSSTVS
                     LSWTAATDNVAVTSYDIYMNSVLKTSVSSSNLTATITGLTASTTYSFYVIAKDAAVNS
                     SPASNTANGTTTIAIPDTQNPTAPTNLAVTGSSSSTVSLSWTASTDNVGVTSYDVYVN
                     SVFKSTVSGTTALVNGLTPSTTYSFYVVAKDAAGNSSAQSNAINGTTTVLTSSCANES
                     FENIPTAASSYGTQTWIGDSALNWTATDARTDQTLNTKAILVRNGSLTASTSPDGIGS
                     LTVTTQQKFTSGNNGTFSLRVNGNIVGTIPFGAPGGLATTTTISNINIAGDVIISITD
                     NSNLATSRVAFDNLSWTCYSGLGLETLSQKDFKIYPNPSNGDFNIVFEDANGTHSVEI
                     FSLLGQKVFEKKNSQTASISVTNLQKGTYLIKVTKDSKSTTKKIIIN"
     gene            1924..>4541
                     /gene="purL"
                     /locus_tag="PLQ70_10175"
                     /gene_synonym="purI"
     CDS             1924..>4541
                     /gene="purL"
                     /locus_tag="PLQ70_10175"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HQE35022.1"
                     /translation="MIHFFENQSKTIFAVQTQSEISTEDISKLNWLFADAHKIEKSVL
                     SDFFVGPRATMITPWSTNAVEITQNMGISGIIRIEEFYKVSEDFTDFDPMLSQKYTEL
                     NQDIFTIHIQPEAILNIGDIAAYNQSEGLSLSVEEVEYLNNLSTKLGRKLTDSEIFAF
                     SQANSEHCRHKIFNGTFVIDGVEKETSLFKLIKKTSQENPNDIVSAYKDNVAFVKGPK
                     VQQFAPKSADKPDFYEIKEFDSVISLKAETHNFPTTVEPFNGAATGSGGEIRDRLAGG
                     QGSLPMAGTAVYMTSYSRLEENRPWEDAVAERKWLYQTPIDILIKASNGASDFGNKFG
                     QPLITGSVLTFEHEENNRKLGYDKVIMQAGGIGYGKLDQAIKHKPQEGDKIVILGGEN
                     YRIGMGGAAVSSADTGAMSSGIELNAVQRSNPEMQKRAANAIRGLVESDHNPIVSIHD
                     HGAGGHLNCLSELVEETGGLIDLDKLPVGDPTLSAKEIIGNESQERMGLVIGQKDIDL
                     LQKIADRERSPMYQVGDVTADHRFTFESKTTGLKPMDYALEDFFGSSPKTVMTDKTVN
                     YNYPALEYDVKNISTYLEQVLQLEAVACKDWLTNKVDRCVGGKVAKQQCAGPLQLPLN
                     NCGVVALDYTGKEGIATSIGHSPIASLIDPVAGTRTAIAESLSNIVWAPIKDGMKGIS
                     LSANWMWACKNEGEDARLYAAVEACSDFAIELGINIPTGKDSLSMKQKYPNDEVIAPG
                     TVIISAGGNCTDIRKVVEPVLQKDGGSIYYINLSQDEFKLGGSSLAQTLNSIGNEAPT
                     IKDAAFFKNAFNTLQELISNNQILAGHDIGSGGLITTLLEMCFADVNLGAKIDFSVFE
                     EKDIIKYLFSENIAVV"
BASE COUNT         1452 a          876 c          898 g         1315 t
ORIGIN      
        1 atgtattctg aaaatccaac aggaattgat ccttacaatt attctacttc tgtatcttca
       61 gacaaatgtg gtagttattc cgttgaaggc gattgttaca atagagaaca tatcatacca
      121 caatcggttt ttaattctaa ctctcctatg gttgccgatg ctcattttat tacacccact
      181 gatggaaaag taaacggtct tagaagcaat tttccacacg gaattgttgc ttcggcatct
      241 aaaactactt taaacggaag caaactcgga actagcggtg tttctggata ttcaggaact
      301 gttttcgaac caattgacga attcaaaggt gatattgcga gaatgtattt ttattttgcc
      361 actcgttatg aaaataatgt tgcaggatat agttatgata tgtttaatgg cacaagcggt
      421 caggttttta caccagaatt tttaagtatg ttattggcct ggcacgcaca agaccctgta
      481 aatgcaagag aaattgctcg taacaatgct atttatgccc gtcaaaacaa tagaaatcca
      541 tttattgatc atcctgaata tgttcaatta atttggaatc caacagccga tacagtagca
      601 ccaaccgctg ctacaaattt aaccgttaca ggaactacat ctagtacggt ttctttgagc
      661 tggacagctg ctactgataa cgttgctgta acaagttatg atatctatat gaatagcgtt
      721 ctgaaaactt cggtttcaag ttcaaatcta acagcgacaa taacaggact tacagcatca
      781 acaacttatt ctttttatgt tattgcaaaa gatgctgctg taaattcatc tcctgcgagt
      841 aatactgcaa atggaacaac aacgattgcc attcctgata ctcaaaaccc aactgcacca
      901 accaatttgg ccgttacagg aagttcttca agtacagtct ctttgagttg gactgccagt
      961 actgataatg ttggagtaac aagctatgac gtttatgtga actccgtttt caaatcaaca
     1021 gtttctggaa ctactgcatt ggtaaatggt cttactccct ccactactta ttctttttat
     1081 gttgtagcca aagatgctgc tggaaattct tcagctcaaa gtaatgcaat aaacggaacg
     1141 accacagtac taacttcttc ttgtgccaac gaaagttttg aaaatattcc taccgctgct
     1201 tcatcctatg gaacacaaac ttggataggt gactcggcgt tgaattggac agcaactgat
     1261 gcacgtacgg atcaaacctt aaacacaaaa gcaattttag ttcgaaatgg ttcactaaca
     1321 gcttcaactt ctccagatgg aataggaagt ttaacagtta ctacgcaaca aaaattcact
     1381 tcaggaaaca acggtacctt ttctcttcgt gtaaacggca atatcgttgg aactattcct
     1441 tttggtgcac ctggaggatt ggcaactaca actactattt ccaatattaa tatcgctgga
     1501 gatgtaatta tcagcattac tgataatagc aacctcgcta caagtcgcgt ggctttcgac
     1561 aatctttctt ggacttgtta ttcaggatta gggcttgaaa ctttaagtca aaaggatttc
     1621 aaaatatatc caaatccatc aaacggtgat ttcaatattg tttttgaaga tgccaatggt
     1681 actcattctg ttgaaatatt ctctttatta ggacaaaagg tttttgagaa aaaaaattca
     1741 caaactgctt cgatatcggt aacaaatctt caaaaaggaa cttacttaat taaggtcaca
     1801 aaagattcca aatcaacaac caagaagatt atcatcaact aaatctaaag cggcaaattt
     1861 gccgcttttt ttttaagtct taattaccta aatttgcacc gcaatacaac aaactacaca
     1921 acaatgatcc atttctttga aaaccaaagc aagactattt ttgcagttca aacgcaaagc
     1981 gaaatttcga ccgaagacat ttcaaaactc aactggcttt ttgccgacgc acataaaata
     2041 gaaaaatccg tattgtcgga tttttttgtt gggcctcgtg ccacgatgat tacgccttgg
     2101 agcaccaatg ccgtggaaat cacccaaaat atgggcattt ctggaatcat tcgaattgaa
     2161 gaattttaca aagtgtcgga agatttcact gatttcgatc cgatgctttc gcaaaaatat
     2221 accgagttga atcaagatat tttcacgatt cacattcaac ccgaagccat cctaaacatt
     2281 ggcgatatcg ctgcttacaa ccaatcggaa ggattgtcat taagcgttga agaagtagaa
     2341 tatttgaata atttgtccac caaattaggc agaaaactga ctgattccga gattttcgct
     2401 ttctctcaag ccaattcaga acactgtcgc cacaagattt tcaacggaac ttttgtgatt
     2461 gacggcgtgg aaaaagaaac ttctcttttc aaattaatca aaaaaacatc gcaggaaaat
     2521 cccaacgata ttgtttcggc ttacaaagac aatgtggctt tcgtgaaagg accaaaagtg
     2581 caacaatttg ccccaaaatc ggccgacaaa cccgattttt acgaaataaa agaatttgat
     2641 tcggttatct ctttaaaagc agaaacccat aattttccaa ctactgtaga gcctttcaac
     2701 ggagctgcaa caggttcggg aggagaaatt cgcgaccgtt tggctggagg acaaggttcg
     2761 ttgccaatgg ctggaactgc tgtttatatg acttcttatt caagattgga agaaaatcgt
     2821 ccttgggaag atgccgttgc cgaaagaaaa tggttgtacc aaaccccaat cgatatcttg
     2881 atcaaagcgt cgaatggagc gtctgatttt ggaaataaat ttggacaacc tttgattact
     2941 ggttcggttt tgactttcga acacgaagaa aacaaccgca agttgggtta cgacaaagta
     3001 attatgcaag cgggaggaat tggttacggt aaactggatc aagccatcaa acacaaacca
     3061 caagaaggcg ataaaatcgt tattctaggt ggagaaaatt atagaattgg aatgggtgga
     3121 gcagcggttt cctctgccga tactggagcg atgagttctg gaatcgagtt gaatgccgtg
     3181 caacgttcga accctgaaat gcaaaaacgt gctgccaacg ccattcgtgg tttggtagaa
     3241 agcgaccata atccaattgt ttccattcac gatcacggag ctggtggaca tttaaactgc
     3301 ctttcggaat tggtggaaga aacgggtggt ttgattgatt tggataaatt acctgtgggc
     3361 gaccctaccc tttcggctaa agaaattatc ggtaacgaat cccaagaaag aatgggattg
     3421 gttattggtc aaaaagacat cgatttactt caaaaaattg cagatcggga acgttcgcca
     3481 atgtaccaag tgggtgacgt tacagcggat caccgcttta cttttgagtc aaaaactacg
     3541 ggtttaaaac caatggatta tgctttggaa gatttcttcg gaagttctcc aaaaacggtt
     3601 atgaccgata aaactgtaaa ttacaattat ccagcattag aatatgatgt aaaaaacata
     3661 tctacttatt tagaacaagt attgcaatta gaagccgttg cttgtaaaga ctggttgacc
     3721 aacaaagtcg atcgttgtgt aggcggaaaa gtagccaaac aacaatgtgc cggccctttg
     3781 caattgccat tgaacaactg tggcgtggtt gccttggatt atacaggaaa agaaggaatt
     3841 gccacctcta ttgggcactc gcctattgct tccttgattg atccggttgc aggaaccaga
     3901 acggccattg ccgaatcctt gtcgaatatt gtttgggctc caatcaaaga cggaatgaaa
     3961 gggatttcat tgtcggcgaa ctggatgtgg gcttgcaaga acgaaggcga agacgctcgc
     4021 ttgtacgccg ccgttgaagc ttgttcggat tttgccatcg aattgggaat caatattcca
     4081 actggaaaag attcgctttc gatgaaacaa aaatatccaa acgacgaagt aattgcgccg
     4141 ggaacggtaa ttatttcggc tggtggaaac tgtactgata ttcgaaaagt agtagaaccc
     4201 gttttacaaa aagacggtgg ttctatttat tatatcaatt tgtctcaaga tgaattcaaa
     4261 ttgggaggtt cttcattggc acaaacttta aacagtattg gtaacgaagc accaactata
     4321 aaagatgcag ctttctttaa aaatgcgttc aatactttac aagaattgat ttcgaataat
     4381 caaatcttgg cgggtcacga tattggaagt ggtggtttaa tcacaacttt actagaaatg
     4441 tgttttgcag acgtaaattt aggagctaaa attgatttct ctgttttcga agaaaaagac
     4501 attatcaaat atttattctc tgaaaatatc gctgtggttt t
//
LOCUS       DAOSNN010000218         4523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_19259_length_4523_cov_4.805506, whole genome shotgun sequence.
ACCESSION   DAOSNN010000218 DAOSNN010000000
VERSION     DAOSNN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19259_length_4523_cov_4.805506"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..554)
                     /locus_tag="PLQ70_10180"
     CDS             complement(384..554)
                     /locus_tag="PLQ70_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35023.1"
                     /translation="MTLTVKELIEKLNEYPEDYLVYTRGHFNDLSNVEYISDQEDLKR
                     IYINTSKPEIKK"
     gene            complement(819..2135)
                     /gene="ahcY"
                     /locus_tag="PLQ70_10185"
     CDS             complement(819..2135)
                     /gene="ahcY"
                     /locus_tag="PLQ70_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQE35024.1"
                     /translation="MSTTTTPYVAFKVKDISLAAWGRKEIELAEAEMPGLMALRAEYK
                     DEQPLKGARIAGCLHMTIQTAVLIETLIALGAEVTWSSCNIFSTQDQAAAAIAAAGIQ
                     VYAWKGLDEESFDWCIEQTLFFGEDRKPLNMILDDGGDLTNMVIDRFPELVAGIKGLS
                     EETTTGVHRLYERVKAGTLPMPAINVNDSVTKSKFDNKYGCKESAVDAVRRATDIMLA
                     GKRVVVCGYGDVGKGTAASFRGAGSIVTVTEIDPICALQAAMDGFEVKKLDTVVGNAD
                     IIITTTGNKDIVLGSHFEQMKDKTIVCNIGHFDNEIDMAWLNKNHGASKIEIKPQVDK
                     YTIAGKDIIILAEGRLVNLGCATGHPSFVMSNSFTNQTLAQIELWKNSAAYNNEVYML
                     PKHLDEKVAMLHLAKLGVELETLRVDQADYIGVPVEGPFKPEYYRY"
     gene            2388..3023
                     /locus_tag="PLQ70_10190"
     CDS             2388..3023
                     /locus_tag="PLQ70_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HQE35025.1"
                     /translation="MPLFKIINITPTTQILIWKVTESFDELVEKVVLKEKTQLRLNGM
                     KSEMHQRAFLSVRMLIQEIGINDYDLHYDEFGKPYLNDHQHISITHSHNFAAIIISEE
                     TVGIDMEMQREKIIRIADKFVNEKELKRLQSFNAEDYIKKLTVKWGAKEAIFKIRNEK
                     GISFKDHIQVKPFEIGEKETTAILEFENLKQAFFIYFEEIENFTLVYAFEK"
     gene            3219..3692
                     /locus_tag="PLQ70_10195"
     CDS             3219..3692
                     /locus_tag="PLQ70_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE35026.1"
                     /translation="MIIRKGNPEDMTGVLSLIQELADFENEPNAVVITVEDLIRDGFS
                     STPLFHVFVAEVESTIVGIALYYYRYSTWKGKTIHLEDLVVKENMRGSGVGFALYSEI
                     MKQGKKDQVRRIEWNVLDWNTPAINFYKKSGAKILDDWRVVQMDEAAIDAFLEKN"
     gene            3752..>4523
                     /locus_tag="PLQ70_10200"
     CDS             3752..>4523
                     /locus_tag="PLQ70_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQE35027.1"
                     /translation="MRVFKFGGASVKDAAGIKNVYDVLQKVGYEDVLLVVSAMGKTTN
                     ALEDVIKNYFEKSEELNASVQEVKKYHNQILLDLFEDEKNEVFAAANTLFSELEYFLA
                     HNKSPNYNFVYDQIVSYGELISTTILSHFMSYMGIKTQWLDVRNFVKTNSNYRDAEVD
                     WELTQKNIAKNVKKKMLNITQGFLGSDENNFTTTLGREGSDYTAGIFAYCLNAESVTI
                     WKDVPGVMNADPRYFENASLLSHISYREAIEMAFYGATV"
BASE COUNT         1533 a          773 c          724 g         1493 t
ORIGIN      
        1 tctacctgag gtgttaagtt ttctccaaag ttttctttaa gtgagtttat gtaagtttca
       61 atattgggtt tcatatttat ttaaattaag attcaaatgg ttttaagtaa tttcaatata
      121 agtgcatttg ttatccataa ggcaatcaaa ttgtccaata aaaacttctt ctttggatat
      181 ttcgaatgta atattttgac ctagaaaaga tccgttattt gtagaaaaga atatgcttgg
      241 tgaataactt attattttga aattttcata acttaagttg ttgatttttg ataataaaac
      301 tattgcttcg tcaattttat taaagaactc attttgagag aaattgcccc tagcgttaat
      361 ataaatattt tccataatta atattatttc tttatttctg gttttgaagt atttatataa
      421 attcttttta agtcttcttg atcagaaata tattccacat tactcaagtc attaaaatgc
      481 cctcttgtat atactaaata atcttcggga tattcattaa gtttttcgat taattctttt
      541 acggttaaag tcattttggt tttaattgtt aaatgagatt acaaatatag ataaaaactg
      601 tcataaaatg aaaacccata agaaattagt atagtgtaaa aataatttag tttataaagg
      661 tttatcctaa actaaaatcc caacaaatat attctcttga tttttaataa ctttacggga
      721 aaccgtaaaa agcatatttt attttaaaaa taaatataat tcaacaaaaa accctcacat
      781 ttctgtaagg gttctatctt aatactaatt tcttaatact aatatctgta atattctggt
      841 ttaaacggtc cttcaacagg aactccaata taatctgctt gatccacacg taaagtttcc
      901 aattcaacac ctagtttagc caagtgcaac atagccactt tttcatctaa atgttttggt
      961 aacatataca cttcattatt gtaagctgcg ctgtttttcc acaattcgat ttgagctaaa
     1021 gtttggttag taaatgagtt actcattaca aaacttgggt gacctgtagc acaaccaagg
     1081 tttaccaaac gaccttcagc caaaatgata atgtcttttc cagcaatagt atatttgtcc
     1141 acttgtggtt tgatttcgat tttcgatgca ccgtggtttt tattcaacca agccatatca
     1201 atttcgttgt caaagtgtcc gatgttacaa acgatagttt tgtctttcat ttgttcgaaa
     1261 tgagaaccca aaacgatgtc tttattccca gttgtggtga tgatgatatc agcatttcca
     1321 acaacagtat ctaatttttt aacttcaaaa ccgtccattg cagcttgcaa agcacaaatt
     1381 gggtcaattt cggtaacagt tacaatagat ccagcacctc taaaagaagc tgctgttcct
     1441 tttcctacgt ctccgtatcc acaaactaca actctttttc cagccaacat aatatcggta
     1501 gcacgacgaa cggcatcaac tgcactttct ttacaaccgt atttgttatc aaatttcgat
     1561 ttggtaaccg agtcattcac gttgatggca ggcattggta atgttccagc ttttactctt
     1621 tcgtacaatc tatgaacacc agttgtagtt tcttcgctca atcctttgat tccagcaacc
     1681 aattcaggaa aacggtcaat aaccatatta gtcaaatctc caccatcatc caaaatcata
     1741 ttcaatggtt ttctgtcttc gccaaagaac aatgtttgtt cgatacacca gtcaaaagat
     1801 tcttcgtcta gacctttcca agcgtaaact tgaattccag cagcagcaat tgcagcagca
     1861 gcctgatctt gagtagagaa aatattgcaa gatgaccaag tcacttcggc accaagagca
     1921 ataagagttt cgatcaaaac cgcagtttga atcgtcatat gcaaacatcc tgcgatacgt
     1981 gctcctttaa gcggttgttc gtctttatat tctgcacgaa gcgccatcaa acctggcatt
     2041 tcagcctcgg ccaattcaat ttcttttctt ccccaagctg ctagagaaat gtcttttact
     2101 ttgaaagcca cataaggcgt agtcgttgta ctcatttata gtatatttgt attgtaaatt
     2161 tttttgcaaa tttacgcaat aactttataa tttaaaaagc gttcgggaat atttatgatt
     2221 tgtttaattt cctaatcttt tgaattgtaa tgaaataatt tttcttagaa gctaatcctg
     2281 ctgttcacta tatcttttgt ggcgaacccc gccacaaaag gatgccgttt ccatcagggc
     2341 taaaacaacc ttcataattc aaaacgccta attcgtaatt taaggaaatg cctttattca
     2401 aaatcataaa cataactccc acgacacaaa tcttgatttg gaaagtaacc gaatcctttg
     2461 atgagttagt agaaaaagta gttttaaaag aaaaaaccca acttcgatta aacggaatga
     2521 agtccgaaat gcaccaacgt gcttttttga gtgttcgtat gctgattcaa gaaatcggga
     2581 ttaacgatta tgatttacat tacgacgaat tcggcaaacc ctatttaaat gaccatcaac
     2641 acatttcaat cacccattcc cataattttg ctgcgataat tatcagcgaa gaaaccgttg
     2701 gaattgatat ggaaatgcaa cgagaaaaaa taattcgcat cgccgataaa tttgtaaacg
     2761 aaaaagaatt gaagcgtttg caaagtttta atgcagaaga ttacatcaaa aaactaactg
     2821 taaaatgggg agccaaagaa gccattttca aaattagaaa cgaaaaagga atcagtttca
     2881 aagatcatat tcaagtgaaa ccattcgaaa taggagaaaa agaaacaact gccattcttg
     2941 aattcgaaaa cctaaaacaa gccttcttta tttactttga agaaatagaa aactttactt
     3001 tggtttatgc tttcgaaaag taaattctcc taaaaattat taacccacaa caattttgtt
     3061 gtgtttcttc aatgaaatcg ttttagcaaa attgaagtca caaatctcgc tttttttgtg
     3121 aaatcaaaac tattttgtgc ctttcgatta tcataattgt atatttgcca agtattttag
     3181 caaattttgt ctttataatt attttcacaa cccaaattat gattatcaga aaaggaaatc
     3241 ccgaagacat gacaggcgta ttaagcctta ttcaggaact agctgatttt gagaatgaac
     3301 caaacgctgt agtcattacg gtcgaggatt taattcgcga tggcttttcc tccactcctt
     3361 tatttcatgt ttttgttgct gaagtcgaat ctactattgt cggaattgct ttgtattact
     3421 accgatattc cacttggaaa ggcaagacga ttcatttaga agatttggtt gtaaaagaaa
     3481 atatgcgtgg ttcaggcgta ggttttgctt tgtattctga aattatgaaa caaggaaaaa
     3541 aagatcaggt tcgaagaatt gaatggaatg ttttagactg gaatacgccc gccataaatt
     3601 tctataaaaa atcgggcgca aaaatccttg acgattggag agttgtacaa atggacgagg
     3661 ctgcaattga tgcgttttta gaaaaaaatt aataaataca aaactccaaa tttggaattt
     3721 ggaattttaa aaaaatagaa ttttaataat tatgagagta ttcaaatttg gtggtgcatc
     3781 agtaaaagat gccgcaggaa taaaaaacgt atatgacgtt ttacaaaaag taggttatga
     3841 agatgtattg ttggtggttt cagcaatggg aaaaacaaca aatgcactgg aagatgtaat
     3901 caaaaattat ttcgaaaaat cggaagaatt aaatgcatca gttcaagaag taaaaaaata
     3961 ccacaatcaa attttgttgg atttatttga agacgaaaaa aacgaagttt ttgccgctgc
     4021 aaatacgctt ttctcagaat tagaatattt cttggcacac aacaaatcac ccaattacaa
     4081 ttttgtttat gaccaaattg taagttatgg agaattaatt tcgaccacta ttttaagcca
     4141 ttttatgagt tatatgggca tcaaaaccca atggttggac gttcgtaatt ttgtaaaaac
     4201 caattcaaat tatagagatg ccgaagtaga ttgggaatta actcaaaaaa atattgccaa
     4261 aaatgtaaag aaaaaaatgc tcaacattac ccaaggattt ttgggttctg acgaaaataa
     4321 tttcaccaca acccttggtc gtgaaggctc tgattacacc gctggaattt ttgcgtattg
     4381 ccttaatgcc gaaagcgtaa ccatctggaa agacgttcct ggagtgatga atgccgatcc
     4441 aagatatttt gaaaatgcga gtttactaag tcatatttca tatcgtgaag ctattgaaat
     4501 ggcgttttat ggagcaaccg tta
//
LOCUS       DAOSNN010000219         4456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_19611_length_4456_cov_4.368780, whole genome shotgun sequence.
ACCESSION   DAOSNN010000219 DAOSNN010000000
VERSION     DAOSNN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19611_length_4456_cov_4.368780"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLQ70_10205"
     CDS             <1..630
                     /locus_tag="PLQ70_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009781037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HQE35028.1"
                     /translation="IGGLSSIKWIMHPDGRFKMELVALKNAENAGGFDGAYFEDKISA
                     FGITFSFPEKEVTGMKWFGKGPYHVWKNRIKGTTYGVWEKAYNTTITGESFENLVYPE
                     FKGYHANLLGANLKAGASSFKVFSESDNLFLRLFTPDLPKNGFPGSNPQPAFPEGDIS
                     FMYEIPAMRDFKPLEQQGPESQPTNIRIKKGDDGIAMNLWFDFRTLTEK"
     gene            650..>1656
                     /locus_tag="PLQ70_10210"
     CDS             650..>1656
                     /locus_tag="PLQ70_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQE35029.1"
                     /translation="MKLIKLLSIVCLAFLLFNFADKKQDKPIVYTVGDSTVKNGAGKG
                     DGGLWGWGDFIGQFLDSSQITVENHALGGTSSRTFQDKGLWEEVFKKLKKGDYVMIQF
                     GHNDDGPLNDDSRARGTIKGIGDETQEIDNMLTKKHEVVHTYGWYIQKVVREAKSKGA
                     IPIVCSPIPRNDWKEGKVPRNTQSYGLWAKQIAEKENVTFIDLNTIMAQKMEKLGESN
                     VTETYFYKRDHTHTSAKGAVMSATSIIEGLKASDNSLKKYILANPKIVLPAKKKVYLI
                     GDSTMANNGNNPNAVGWGVEFPKYCDTTRIEVINKARGGRSTRTFVKEGLWDAVKNQL
                     KP"
     assembly_gap    1657..1756
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1929..3266
                     /locus_tag="PLQ70_10215"
     CDS             1929..3266
                     /locus_tag="PLQ70_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQE35030.1"
                     /translation="MMKDISIIIALFLTSIFSYGKDYYVAPNGNDSNIGSKDQPVETI
                     KRAQELASSGDTVYIRGGLYSMREDQIASYQRAYAYVTQLDKNGISYLAYANEIPVFD
                     YKNVKPTNKRVVAFFVKGDHIHVKGIEVIGVQVTITGHTQSECFEVQGSNNTLEQLKM
                     HDGMAIGVYMLSGSNNLILNCDAYNNWDSVSEGAKGGNTDGYGAHLKKGSVNNIFRGC
                     RAWFNSDDGFDLINNAEPVLIENCWAFYNGLTSDFVSRGDGNGFKVGGYAKGRRPYND
                     VVENFTPIPKNTIRFCLAVGNKQGGFYANHHLEGNYWYNNTAYKNRVNYDMLNCLALN
                     PTDFGTDGPGWNHEMVNNLGFAAKAKELGNIDKSRCVLKTNYFDLNVSSSDFLSLDES
                     FLTEPRQADGSLPKTDFLKLAPSSKLINAGTNIGFPFQGKAPDLGCFESDASH"
     gene            3333..>4456
                     /locus_tag="PLQ70_10220"
     CDS             3333..>4456
                     /locus_tag="PLQ70_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HQE35031.1"
                     /translation="MKNSILIITLFLSSLYSFGATFYVAPNGVDTNAGDLNNPVETIQ
                     KAQALASSGDTVYIRGGLYSMREDQIARYNSIWAYVTELNKNGISYLAYPNETPIFDY
                     KNIKPADYRIFAFFITGSNIHIKGIEVVGVQVTILTHTQSECFDIQGSNITLEQVKMH
                     DGMAIGVYMTKGSNNLILNCDAYNNYDSVSESGVGGNTDGFGFHLKKGSVNNVIRGCR
                     AWYNSDDGYDLINNAEPVLIENCWAFNNGYSISGTNKFVSRGDGNGFKAGGYGAGGTP
                     YTTIQSSYTPIPKNTIRFCLAVRNKQSGFYANHHLDGNYWYNNTAYVNKRNYNMLNCV
                     ALNATDFATDGPGWNHTLANNLGFAATTAELENIDKPRCF"
BASE COUNT         1467 a          742 c          894 g         1253 t
ORIGIN      
        1 attggtggtt tatcgtccat aaaatggatt atgcacccag acggtagatt taaaatggaa
       61 ttagtcgctt tgaaaaatgc cgaaaatgct ggcggttttg acggcgcata ttttgaagat
      121 aaaattagtg cgttcggaat cacgtttagt ttccctgaaa aagaagttac ggggatgaaa
      181 tggtttggaa aagggcctta tcacgtttgg aaaaacagaa ttaagggaac gacttatggt
      241 gtttgggaaa aagcatacaa cacaactata acgggcgaaa gtttcgaaaa tttggtttat
      301 ccagaattca aagggtatca cgccaattta ttgggagcca atctaaaagc aggagcttca
      361 tcatttaaag tatttagcga atctgataat ttatttttgc gattattcac tcctgatttg
      421 cctaaaaatg gtttccccgg aagcaatccg caacctgctt ttccggaagg cgacatttcc
      481 tttatgtatg aaattccggc tatgcgtgat ttcaaaccct tggaacaaca aggaccagaa
      541 agccaaccta caaacattcg aatcaaaaaa ggcgacgacg ggattgcaat gaatctttgg
      601 ttcgatttta gaacattaac tgaaaaataa aaaatttaca tcttatacta tgaaactgat
      661 taaactactt tccatcgtct gtttagcatt tttgctattt aattttgcag ataaaaaaca
      721 agacaaacca attgtttata ctgttggaga ttcaaccgta aaaaatggcg ctggcaaagg
      781 agacggcgga ctttggggct ggggtgattt cattggccaa tttttggatt cttctcaaat
      841 aacggttgaa aaccacgctt tgggtggcac cagcagccga acttttcaag acaaaggttt
      901 atgggaagaa gttttcaaaa aactcaaaaa aggcgattat gtaatgatcc aatttggtca
      961 caatgacgat ggtcctttaa atgatgattc cagagccagg ggaacgatca aagggattgg
     1021 agacgaaacc caagagattg ataatatgtt gaccaaaaaa cacgaagtgg ttcacactta
     1081 cggttggtat atccagaaag tagttcgtga agccaaatca aaaggagcta ttcctattgt
     1141 ttgctcgcct attcccagaa acgattggaa ggaaggaaaa gtgccgcgca acacccaatc
     1201 gtatggatta tgggccaaac aaattgccga aaaagagaac gtgactttta tcgatttgaa
     1261 taccataatg gctcaaaaaa tggaaaaatt aggagagtcg aatgttacgg aaacttattt
     1321 ttacaaaaga gatcataccc atacttctgc caaaggagcc gtgatgtcgg ctacttcaat
     1381 tattgaaggt ttgaaagcga gtgataattc attaaaaaaa tatattctag ccaatccaaa
     1441 aattgtactt cctgccaaga aaaaagttta tttgattggc gattcgacaa tggccaataa
     1501 tggtaacaat ccaaatgccg tgggttgggg agttgaattt ccaaagtatt gcgacacgac
     1561 aagaatcgag gttatcaata aagcccgtgg cggaagaagc acccgaactt ttgtaaaaga
     1621 aggattgtgg gatgcggtaa aaaatcaatt gaaaccnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnaaag catttttccc aaaagaccac acccataccg gtgttgaagg
     1801 agccaatctc aatgcactaa ccgcagcgga aagcctcaaa aaaataaaag aatgtggctt
     1861 gaaagagtat atcgaaattc ctaaagacaa tactaagcaa gaatccaaat aaaaagtaca
     1921 ttcaccaaat gatgaaagat atctctataa ttattgcact ctttttaact agtatttttt
     1981 cctatggaaa agattattat gttgcaccaa atgggaatga ttccaatatt ggtagtaaag
     2041 accagcctgt tgagaccata aaaagagcac aagaattagc cagttcggga gatactgttt
     2101 acatcagggg tggactttat tcgatgaggg aagatcaaat agcgagctat caacgagctt
     2161 atgcttatgt cacacaacta gacaaaaacg gcatcagtta tttggcttat gctaacgaaa
     2221 ttccagtatt tgattataaa aacgttaaac ctacaaataa acgtgttgtc gctttttttg
     2281 tcaaaggaga tcatattcac gttaaaggca ttgaagttat tggtgttcaa gtaaccataa
     2341 cagggcatac tcaatccgaa tgttttgagg ttcaaggcag caacaacaca ttggaacaac
     2401 tgaaaatgca cgacggaatg gctataggag tttatatgct ttcgggttcc aataatctca
     2461 tcttaaattg tgatgcctac aacaactggg attctgtttc agaaggagca aaaggaggaa
     2521 atactgatgg ttatggcgca catctcaaaa aaggaagtgt caataacatt ttcagggggt
     2581 gccgagcttg gtttaacagc gatgatggat tcgatttaat caacaatgca gaacccgttt
     2641 taatagaaaa ttgctgggct ttttataatg gtttaacatc cgattttgtg agtcgtggtg
     2701 atggcaatgg gtttaaagtt ggaggttatg ccaaaggtag aaggccttat aatgatgtcg
     2761 tggagaattt caccccaatt cccaaaaaca ccatccgttt ttgtttggct gtcggaaaca
     2821 aacaaggtgg tttttatgcc aatcatcatt tagaaggaaa ttattggtac aacaacaccg
     2881 cttataaaaa ccgagtaaac tacgatatgt taaattgttt agccctaaat ccaaccgatt
     2941 ttggtactga tggcccaggt tggaatcacg aaatggtcaa caacttaggt tttgctgcta
     3001 aagcaaaaga attaggaaat attgacaaat cacgttgtgt gttgaaaacg aattattttg
     3061 atttaaatgt ttcttcatcg gattttctaa gccttgacga aagttttcta acagagccaa
     3121 gacaagcaga tgggagtctg cctaaaacag actttttaaa attagctcct tccagtaaat
     3181 taatcaatgc aggaactaat attggatttc catttcaagg taaagctccc gatttgggtt
     3241 gttttgaatc cgatgctagt cattagaaca ttaagatttt taaaataatt gccatcaaat
     3301 tgtttcaata cttttatgta aaatttcaga atatgaaaaa tagtatttta atcatcaccc
     3361 tatttttaag tagtttgtat tctttcggag caacttttta cgtcgctcca aatggtgtcg
     3421 ataccaatgc tggagaccta aacaatcccg ttgaaacaat tcaaaaagca caagcattgg
     3481 ctagttcagg ggatacggtt tatataagag gcggacttta ttctatgagg gaagaccaaa
     3541 ttgctcgtta taatagtatt tgggcttatg taacagaact taataaaaat ggcatcagtt
     3601 atttggctta tcccaatgaa actccaattt ttgattataa aaatattaaa cctgctgatt
     3661 atcgcatatt tgcctttttc attacaggaa gtaatattca tattaaaggg attgaagtag
     3721 ttggtgttca agtaacgata ttgacccata cccaatccga atgttttgat attcaaggaa
     3781 gcaatatcac gcttgaacaa gtaaaaatgc acgatggtat ggctattgga gtctatatga
     3841 caaaaggctc caataacttg attctaaact gtgatgccta taataattat gattctgttt
     3901 ccgaaagtgg agtaggcgga aatacggatg gttttggttt tcacttaaaa aaaggaagtg
     3961 tcaataatgt gattagagga tgtcgtgctt ggtacaatag cgacgatggt tatgatttaa
     4021 tcaataatgc ggagcctgtt ttaattgaaa actgttgggc ttttaataat ggctattcca
     4081 ttagtggaac caataaattt gtaagtcgtg gcgacggaaa tggtttcaaa gccggtggat
     4141 atggtgctgg cggaactcct tataccacta ttcaatccag ttacacgccc attccaaaaa
     4201 acactattcg attttgtctg gcggtcagaa acaaacaaag cggtttttat gccaaccacc
     4261 atttagacgg caattattgg tacaacaaca cggcctatgt taacaaaaga aactacaata
     4321 tgttgaattg cgtagcatta aatgcaaccg attttgccac tgatggccct ggatggaatc
     4381 ataccttggc aaataattta ggttttgccg ctaccacggc cgaattggaa aatatagata
     4441 aaccacgttg cttctt
//
LOCUS       DAOSNN010000220         4421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_19807_length_4421_cov_3.541686, whole genome shotgun sequence.
ACCESSION   DAOSNN010000220 DAOSNN010000000
VERSION     DAOSNN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19807_length_4421_cov_3.541686"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..3596
                     /gene="cas9"
                     /locus_tag="PLQ70_10225"
     CDS             207..3596
                     /gene="cas9"
                     /locus_tag="PLQ70_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HQE35032.1"
                     /translation="MAKILGLDLGTNSIGWALIDDKLNKILGIGSRIFPMGVENLGDG
                     DNEMSKNASRTGARGVRRQFFRRRLRKKVLLKSLSENNMCPMSLKDFEDWKQTKQFPS
                     EKLATWFAMNPYELRQKALNEKLSLEEIGRIFYHLIQRRGFLSNSRKGGSDDGAIFKG
                     NAKEGKIGIDDTLESIEGKTLGSYLYEIYPKENQPFQDGLERIRNRYTTRKMYIDEFE
                     LIWDKQSQFHSVLNYDLKTTFGGRKQDGYKEDGIIFHQRPLRSQKHLVGNCSFEPTKT
                     KCPLSAIPNEKRRAWEWVNTVEYNGKKISQGEKEKMIEILYSIPLGKEKVDFKVLRKA
                     IGKESAEFKFNYKDDDKIVGTHTISNLSNKKFFGKKWFDFSEKEQEDIWHVLYFFDSK
                     SNLKDYAIKNWSFTEEQAIAISKFNVKDGYASLSRKAIGNILPFLQQGYTYDVAVVMG
                     GIKNSFGDKWQNDTAENNQKQLDLIDNVYDIVRAKISGGFIDTVKDLLRKEFQFNNKQ
                     LKKLYHHSATIDVTALLEKLPIGKEADKEIQAIRNPIVITALFELRKLVNELIEEHGQ
                     LDEIKVEMARDLKISKSQRNKIRRDQKRLERENDRVKTEVEKYTRVSHDNILKFKLWE
                     ECKQTCPYTGVSIPVSKLFTGEIQIEHIHPWSRSLNDSFNNKTLCYADENRRKGDKTP
                     FEFYGNDELDWIARKERALKLFSDTKEYPNAYQKFKRFVQQKFDDDFSSRQLNDTRFI
                     SKEASTYLTQICKKVNVSPGQMTANLRQKWGLNTVLNDENAKTREDHRHHAIDALVMA
                     CGKTSYLQELSKWNRYNRNYDLKDFPMPWETFRFDAEKAVDKILISHKRISNDITIRY
                     TKVEKNGKTHINKGVAARGQLHKEFVYGKRTAPFSDEAYHIRKSIDSLTTEKQLEKVV
                     DEVIKKLIHKRIQELGGFVKGTIPVNTFFIVDENGVKQPQILLPNKNGSPVPILKVRM
                     KENFSGAEKLKDDVNQWVNPRNNHHVLIYKDEKGNLKEEVVTFWTVVERKSKGFPTYQ
                     LPAEGKEIVTALHINDMFLLGLNEDEINWVNPDYEILRDSLYRVQKLTSGDYFFRKHK
                     SSTVTDDDYKQIRGFGDGKTGWFTFNPIKVKVSVSGKISRI"
     gene            3647..3892
                     /locus_tag="PLQ70_10230"
     CDS             3647..3892
                     /locus_tag="PLQ70_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HQE35033.1"
                     /translation="MTRQSITLAKQNDEWLKTQIAEEEFTSKSEAVNYLIKQARERDA
                     HIEFVRMKLDVAEKSGFAKKQTREEMLAEFKKNLPNV"
     gene            3885..4160
                     /locus_tag="PLQ70_10235"
     CDS             3885..4160
                     /locus_tag="PLQ70_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019038731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQE35034.1"
                     /translation="MYSYYLSSEAKEDLRRIYYYGVGQFGIDQADHYFNMIHDCFDRI
                     EKDPFLFPSAEHIKKGYRYCVCGVDTIYYKINENEIEIITIIGRQNF"
BASE COUNT         1704 a          571 c          857 g         1289 t
ORIGIN      
        1 gcaaattaca aaaaaaaagt cccaccgaag caggactaaa gattttacat ctacggcata
       61 tagccttatt gtgataagat taaagattag aaattttaat ccttacaaat gtaaataaaa
      121 aaaactttag tttgaatgag gaaaaccgta aaacagttaa ttattttctt cttaactttc
      181 aaaattcttt aaaaagacag attattatgg ctaaaatatt aggtttagat ttaggaacaa
      241 actcgattgg gtgggctttg attgatgata agttgaataa aattttaggc attggtagta
      301 gaatttttcc aatgggggtt gagaatttgg gtgatggaga taatgaaatg tctaaaaatg
      361 caagtcgaac aggagcaaga ggcgttcgtc gtcaattctt tagaagaaga ttaagaaaga
      421 aagtgttact aaaatcactt tcagaaaata atatgtgtcc aatgagcttg aaagattttg
      481 aagattggaa acaaaccaaa caatttccct ctgaaaaatt agccacttgg tttgcaatga
      541 atccttatga attgcgccaa aaagcattga atgagaagtt gtctttagaa gaaattggta
      601 gaatttttta ccatttaatt caaagaagag gtttcttaag taatagcaga aaaggaggaa
      661 gtgatgatgg agcaattttt aaaggaaatg ctaaagaagg aaaaattgga attgatgata
      721 ctctagaaag tattgaaggt aaaactttag gttcttattt gtatgagatt tatccaaaag
      781 aaaatcagcc ttttcaggat ggattagaaa gaatccgaaa tagatacacc actcgtaaga
      841 tgtatatcga tgaatttgaa ttgatttggg ataaacaatc tcagtttcat tcggttttga
      901 attatgattt aaaaacaact tttggaggaa gaaagcagga tggttacaaa gaagatggaa
      961 ttatttttca tcaaagacct ttgcgttcac aaaagcattt ggttggtaat tgttcttttg
     1021 aacctacaaa aacgaaatgt ccattaagtg ctattcccaa tgaaaaacgt agagcttggg
     1081 aatgggtaaa tactgttgaa tataatggca agaaaatttc tcaaggagaa aaagagaaaa
     1141 tgattgaaat cttatattca attccattgg gaaaagaaaa agtagatttt aaagttttaa
     1201 gaaaagccat tggtaaagaa agtgctgaat tcaaattcaa ttataaagac gacgataaga
     1261 tagttggcac acatactatt tctaatttat cgaataaaaa attctttggc aaaaaatggt
     1321 ttgatttttc agaaaaggag caggaagata tttggcacgt tttgtatttt tttgacagta
     1381 aatcaaatct taaagattat gctattaaga attggagttt tacagaagaa caagcaattg
     1441 caatttctaa atttaatgta aaagacggtt atgccagttt gagccgaaaa gccattggta
     1501 atattttacc ttttctacag caaggttata cctatgatgt agcagtggtt atgggtggaa
     1561 taaaaaatag ttttggcgac aaatggcaaa atgatacagc agaaaacaat caaaaacaat
     1621 tagatttaat tgataatgtt tatgacattg ttagggctaa aatttcgggt ggatttatag
     1681 atacagttaa agatttgctt cgaaaagaat ttcaattcaa taacaaacaa ttaaagaaac
     1741 tctaccatca ttcggcaaca attgatgtta ccgcattatt agaaaaatta ccaattggca
     1801 aagaggctga caaagaaata caagctattc gaaatccaat cgtaataact gctttatttg
     1861 agttgcgaaa attagtcaat gaattaattg aagaacacgg acaactcgac gagataaaag
     1921 tggaaatggc tcgtgatttg aaaatctcca aatcgcaacg aaataaaatc cgtagagatc
     1981 aaaaacgatt agaacgagaa aatgacagag taaaaaccga agtggaaaaa tacacgagag
     2041 tttctcacga caatatttta aaattcaaac tttgggagga gtgcaagcaa acttgtccat
     2101 atacaggtgt ttcaattcct gttagtaaac tttttacggg tgagattcaa atagaacata
     2161 ttcatccgtg gagtcgttcg ttaaacgata gtttcaataa taaaacatta tgctatgctg
     2221 atgagaacag acgaaaagga gataaaacac cttttgagtt ttacggaaat gatgagttgg
     2281 attggattgc cagaaaagaa cgagcattaa aattattttc ggacaccaag gaatatccaa
     2341 atgcttacca aaaattcaaa cgatttgtac aacaaaaatt tgatgatgat ttttcttcaa
     2401 gacagttaaa tgacacccga tttataagca aagaagcttc aacctatttg acgcaaattt
     2461 gcaaaaaagt aaatgtttcg ccaggtcaaa tgacagccaa cttgcgccaa aaatggggtt
     2521 tgaatacggt tttgaatgat gaaaatgcta aaacccgaga agaccatcgc caccacgcca
     2581 ttgatgcttt ggttatggct tgtgggaaaa caagttattt acaggaattg tctaaatgga
     2641 atcgttacaa taggaactac gatttaaaag attttccaat gccttgggaa accttccgat
     2701 ttgatgcaga aaaagcagtg gataaaatat tgatttcgca taaacgtata tctaatgata
     2761 taactattag atataccaaa gtagagaaaa acggaaaaac gcatatcaat aaaggggttg
     2821 ctgcacgagg acaattgcat aaagagtttg tttatggcaa aagaacagct ccgtttagtg
     2881 atgaagctta ccacataagg aaatcaatag atagtttaac tactgaaaaa caacttgaaa
     2941 aagtagttga tgaagtgatt aaaaagttaa ttcataaaag aattcaagaa ttaggtggat
     3001 ttgtaaaagg aactattcct gtaaacactt tttttattgt tgatgaaaac ggagtgaaac
     3061 agccacaaat tttattgccg aataaaaatg gttcaccagt tcctattttg aaagtaagaa
     3121 tgaaagaaaa ttttagtgga gcagaaaagt taaaagatga tgtaaatcaa tgggtaaatc
     3181 ctagaaataa tcatcacgta cttatttaca aagatgaaaa aggaaattta aaggaagaag
     3241 tggtaacttt ttggactgtt gtggaacgaa aaagcaaagg atttccaacg tatcaattgc
     3301 cagctgaggg aaaagaaata gtaacagctt tgcatattaa cgatatgttt ttgttgggac
     3361 taaatgagga tgaaattaat tgggtgaatc cagattatga aattttaaga gatagtttgt
     3421 acagagttca aaaattaaca tctggagatt atttttttag aaaacataaa tcttcaacgg
     3481 ttacggatga tgattacaaa caaataaggg gttttggaga tggtaaaaca ggatggttta
     3541 catttaatcc aatcaaagta aaagtttcag tatcaggaaa aatttcaaga atatagtttt
     3601 attttaaaaa gttggtgtaa atttacacca actaaaaaat attgttatga cacgacaaag
     3661 tatcacttta gccaaacaaa acgatgaatg gcttaaaaca caaattgcag aagaagaatt
     3721 tacgagtaaa agcgaagcgg taaattattt aatcaagcaa gcgcgggagc gagatgctca
     3781 tatagaattt gttagaatga agttggatgt tgcagaaaaa agcggttttg caaaaaagca
     3841 aaccagagaa gagatgttag ccgaattcaa aaagaatctg cctaatgtat agctattatt
     3901 taagtagcga agccaaagag gatttaagaa gaatttatta ttatggtgtt ggtcaatttg
     3961 gcatcgatca agcagaccac tattttaata tgattcacga ttgttttgat agaatagaga
     4021 aagatccttt tttgtttcct tctgccgagc atatcaaaaa aggctatcgc tattgtgttt
     4081 gtggcgtgga caccatttat tacaaaatca acgagaatga aattgaaatc attaccataa
     4141 ttgggagaca aaatttttag tttcaaaagt tctttgacat aagtttttca aactcttaaa
     4201 catctcaaac cacaactgaa aggtatggca tccgcccaga agggatgcag tagctttcag
     4261 ttgtggtttg attaaagatt agaaaacacg atatttcgaa acgtttaaaa tagcaagggc
     4321 tgcaggttgt ggtttgatta aagattagaa aacacgatat tcatcttcgt cggctgtttc
     4381 tatccactta tgttgtggtt tgattaaaga ttagaaaaca c
//
LOCUS       DAOSNN010000221         4356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_20183_length_4356_cov_5.144385, whole genome shotgun sequence.
ACCESSION   DAOSNN010000221 DAOSNN010000000
VERSION     DAOSNN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20183_length_4356_cov_5.144385"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLQ70_10240"
     CDS             <1..561
                     /locus_tag="PLQ70_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQE35035.1"
                     /translation="PNPAALKFVVSRMLTKSPVEFKNIDETTASPLAKELFKFPYVKE
                     LFMDENYISVTKYEINNWDEITLELRTFIKQYIENGGTVLDESLIKANAEQEKTKNQN
                     FDNLDVTSQQIINILEEYVKPAVQADGGNIAFDSYDETNKIVNVTLQGACSGCPSSTF
                     TLKSGIENMLKSMLNDENIKVEAVNG"
     gene            685..2703
                     /locus_tag="PLQ70_10245"
     CDS             685..2703
                     /locus_tag="PLQ70_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQE35036.1"
                     /translation="MNQLSQETSPYLLQHANNPVHWKAWNANSLAEAKEKNSLIIISI
                     GYSACHWCHVMEHESFEDQEVADVMNKNFVNIKVDREERPDVDAVYMKAVQIMIRRGG
                     WPLNVVTLPDGRPIWGGTYFRKEEWMQTLGQLQEVYQSNPDKVIDYAERLHEGIQSLN
                     IIENLKSGTSVNQEDLTLLVEKWKEEFDLEFGGMSRSPKFMMPNNYQFLLRFAFQKKD
                     MNLFDFVNLTLTRMAYGGVFDTVDGGFSRYSVDNKWHVPHFEKMLYDNGQLVSLYSDA
                     YKLTQNPLYKEVVIKTLSFVERELMNAENGFYCALDADSLNAENHLEEGAFYVWKIPE
                     LKTIIGDDFELFSQVFNINTFGLWEDGNYVLIQNKSLKDIAELNHIKVSSLEKKKIFW
                     EKILYLKREKRSKPRLDDKCLTSWNAIMLKGFVDAYKALEDKNYLKLALQNANFIIKN
                     LWSSEGNLYHNYKNKKSTINGYLEDYCFVIAAFISLYEVTFDEKWLQKAKQLTDYSLE
                     HFYDETSGFFAFTSNLDEALISSHFETEDNVIPASNSVMGKNLFQLSIYFNNSYYEKV
                     SQRMLQNIISFIEYPSAYSNWMDLALNYSEENQELAICGDLALDYLSQINKIYTPNIV
                     LAGTEKKSNLPFLKERFNESKTLFYLCQNKTCLKPTTDFQQIINEMIK"
     gene            2753..>3007
                     /locus_tag="PLQ70_10250"
     CDS             2753..>3007
                     /locus_tag="PLQ70_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HQE35037.1"
                     /translation="MGLSSFFKNLFGSAKKTADEIATSAETAFEEAKVAATPYIDKAE
                     AFAEETFEKVKETATPIIEKTKEAAAPIIEKAEVYADQAQE"
     assembly_gap    3008..3107
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3223..4047
                     /locus_tag="PLQ70_10255"
     CDS             3223..4047
                     /locus_tag="PLQ70_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQE35038.1"
                     /translation="MTVNPEHIANYAETFIKVLIDYSPKLISAFLILFVGLYVIRVIN
                     RLIRRIMVKRELDPTLTKFLADILLWILRVLLFVTFISKLGIETSSFVAILGAMGLAV
                     GLSLQGSLSNFAGGMLIILFKPFKVGDTIEAQGNIGTVAEIQIFVTKLINGNNQTIFV
                     PNGSLSNGTIINYSLQGIRRADLTLSISYDTDIKKAKDIITEVLNNNPKVLKTPEAEV
                     SVKLLTDSSIQLAVRPWANNADFGAVSSDTLENCKLAFDAAGIVIQPFVKEVSKNN"
     gene            complement(4052..>4356)
                     /locus_tag="PLQ70_10260"
     CDS             complement(4052..>4356)
                     /locus_tag="PLQ70_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQE35039.1"
                     /translation="VYSAIFTPNFENKRAVQAEIITENSFEDLQETLYFVGDCAEKCK
                     PILTKENHVFLEEIKYPSAREMSYLSFDKYKKNDTVDVAYFEPYYLKDFMMTVSKK"
BASE COUNT         1495 a          716 c          757 g         1288 t
ORIGIN      
        1 ccaaatccag ctgctttgaa atttgtagta agcagaatgt tgactaaaag ccctgttgaa
       61 ttcaaaaata ttgatgaaac gacggcttct ccattggcaa aagaattatt caaatttccg
      121 tatgtaaaag aactttttat ggatgaaaat tatatttctg taaccaaata tgaaatcaat
      181 aactgggatg aaatcactct agaattgaga acattcatta aacaatatat cgaaaatggc
      241 gggacagttc ttgacgaaag tttaatcaaa gccaacgctg aacaggaaaa aactaaaaat
      301 caaaacttcg ataatcttga tgtcacttcg caacaaatca tcaatatttt agaagaatat
      361 gtaaaaccag ccgttcaagc cgatggaggg aatattgctt ttgactctta tgacgaaaca
      421 aataaaattg tcaatgtaac tcttcaaggt gcttgtagtg gatgtccatc atcaacattt
      481 actctaaaaa gtggcattga aaatatgttg aaaagcatgc ttaatgacga aaacatcaaa
      541 gttgaagctg taaatggata aattcgggtt agccctaaca agttttggaa acctgttagg
      601 gcttagctga aaatcattaa acaacacaat tagcattccg atttcagcat tcagtcactt
      661 caaactgaac aaatacttaa aaaaatgaat caactttcac aagaaaccag tccgtatttg
      721 ctgcaacacg ctaataatcc tgtgcattgg aaggcttgga atgccaattc attggctgaa
      781 gccaaagaaa aaaatagcct tatcattata agtattggct attcggcttg ccattggtgt
      841 cacgttatgg agcacgaaag tttcgaagat caggaagttg ctgatgttat gaacaaaaat
      901 ttcgtcaaca tcaaagtcga tcgcgaagaa cgacctgatg tggatgccgt ttatatgaaa
      961 gccgttcaaa taatgattcg tcgtggtgga tggcctttga atgtggtgac acttcctgat
     1021 ggaagaccta tttggggcgg cacttatttt agaaaagagg aatggatgca aacgcttggt
     1081 caattgcaag aagtttatca atcgaatcct gataaagtga ttgattatgc cgaaagatta
     1141 cacgaaggaa ttcaatcgct gaatattatc gaaaacttga aatcagggac ctccgtaaat
     1201 caagaagatt taactctatt agtcgaaaaa tggaaagaag aatttgattt ggaatttgga
     1261 ggaatgtcaa gatctcccaa atttatgatg cccaacaatt atcagttttt gttgcgtttt
     1321 gcattccaaa aaaaagatat gaatctgttc gattttgtaa atctaacctt aactcgaatg
     1381 gcttatggag gagtttttga caccgtagat ggtggatttt ctcgctattc ggtagataat
     1441 aaatggcacg ttcctcattt cgaaaaaatg ctttacgaca acgggcaatt ggtttctttg
     1501 tacagcgatg cttataaatt gacccaaaac ccattatata aagaagtagt cataaaaaca
     1561 ctttctttcg ttgaaagaga attaatgaat gccgaaaacg gattttattg tgcgctcgat
     1621 gctgatagct tgaatgccga aaatcatctt gaagaaggag ccttttatgt ttggaaaatt
     1681 ccagaattga agacaatcat tggagatgat ttcgaattat ttagtcaggt tttcaatatc
     1741 aatacttttg ggttatggga agacgggaat tatgtcttga tacaaaacaa aagcttgaaa
     1801 gacattgcag aactcaacca tatcaaagtt tcaagcctcg aaaaaaagaa aattttctgg
     1861 gagaaaatac tctatctcaa aagagaaaaa cgttctaaac ccagattaga tgacaagtgt
     1921 ttgacttctt ggaatgctat aatgctcaaa ggatttgttg atgcctacaa agctttagaa
     1981 gacaaaaact atttaaaatt agctttacaa aacgccaatt tcatcatcaa aaatctgtgg
     2041 tcaagcgagg gcaatttgta tcataattac aaaaacaaaa aaagtaccat caacggatat
     2101 ttagaagatt attgctttgt aattgctgct tttatcagtt tgtatgaagt aacttttgac
     2161 gaaaaatggc ttcaaaaagc aaagcaatta accgattaca gcttagaaca tttttatgac
     2221 gaaacatcag gtttttttgc ctttacttcc aatctagacg aagctttgat cagttcgcat
     2281 tttgaaacag aagacaatgt aattccggcc tcaaactcgg ttatgggtaa aaatctattt
     2341 cagttgagta tttatttcaa taattcctat tacgaaaaag taagtcaacg catgttgcaa
     2401 aatataattt cgtttataga atatccttct gcatattcga attggatgga tttggcattg
     2461 aattactctg aagagaatca ggaattagct atttgtggtg acttggcatt agactatttg
     2521 tctcaaatca ataaaatata tacacccaat atcgtcttag ctggaacaga aaaaaaatca
     2581 aatcttccgt tcttaaaaga gcgatttaac gaatcgaaaa ctctatttta tttatgtcaa
     2641 aacaaaactt gtttgaagcc aacaactgat tttcagcaaa ttattaacga aatgataaag
     2701 taaaaccaaa aaaaattgct tactattgta acctaattaa aaacgaaaaa ctatgggttt
     2761 atcatcattt tttaaaaatt tgtttggctc agcgaaaaaa actgccgacg aaattgcaac
     2821 tagcgcagaa actgcttttg aagaagccaa agttgccgca actccttata tagacaaagc
     2881 cgaagcattt gccgaagaaa catttgaaaa agtaaaagag actgcaacgc caataattga
     2941 aaaaaccaaa gaagctgctg cacctattat tgagaaagca gaagtttatg ccgatcaagc
     3001 tcaggaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa acaaaaattg
     3121 aaaaagacca aatttaattt caaaaaaaag ggaataaatt ttacttttgc caaacaaaca
     3181 tataccttct cctattgaga aggtttttta ttaaacaaaa aaatgacagt caatccagaa
     3241 catattgcca actatgctga aacttttatc aaagttttaa ttgattattc gccaaaatta
     3301 atttcggctt tcctgattct atttgtcgga ctttatgtaa ttcgagtaat caacaggctc
     3361 attaggagaa ttatggtaaa aagagaatta gacccaacat taaccaaatt tctcgctgac
     3421 attctgcttt ggattttgag agtcttgttg ttcgtgactt ttatttctaa attaggaatc
     3481 gaaacctcct cttttgttgc catcttaggc gcaatgggac ttgcggtagg attgtcactt
     3541 caaggttcct tgtctaattt tgccggtgga atgttgatta ttcttttcaa accattcaaa
     3601 gtgggtgaca cgatcgaagc acaaggcaac attggaacag tagccgaaat tcaaatattc
     3661 gtaaccaaat taatcaacgg gaacaatcaa accatttttg ttccgaatgg ttcgttgtcg
     3721 aatggcacga ttatcaatta ttctttgcaa ggaatccgaa gagccgattt gacgctttcc
     3781 atttcttacg acaccgacat taaaaaagca aaagacatta ttaccgaagt tttaaacaac
     3841 aatccgaaag ttcttaaaac ccccgaagcc gaagtttcgg taaaactttt gaccgacagt
     3901 tcaattcaac tggccgttag accttgggca aacaatgccg attttggtgc ggtttcttct
     3961 gacactttag aaaattgtaa attagctttt gatgccgctg gaattgtgat tcagcctttt
     4021 gtgaaagagg tttctaagaa taattaaaaa attatttctt cgaaacggtc atcataaagt
     4081 ctttcaaata ataaggttca aaataagcga catccacagt gtcgtttttt ttgtatttat
     4141 cgaaacttaa gtaactcatt tcccttgccg aaggatattt gatttcttcc aaaaaaacat
     4201 gattttcctt ggtcaaaatt ggtttgcatt tctcggcaca atcaccaaca aaataaaggg
     4261 tttcttgtaa atcttcgaaa gagttttcgg tgatgatttc cgcttgaact gctcttttat
     4321 tttcaaaatt tggagtgaaa attgcactgt acactt
//
LOCUS       DAOSNN010000222         4352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_20203_length_4352_cov_5.071911, whole genome shotgun sequence.
ACCESSION   DAOSNN010000222 DAOSNN010000000
VERSION     DAOSNN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20203_length_4352_cov_5.071911"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1458
                     /locus_tag="PLQ70_10265"
     CDS             25..1458
                     /locus_tag="PLQ70_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE35040.1"
                     /translation="MLNKIKTFKYIVPLGICLAVASCTPVLAPLAETKAVPESFGKST
                     DTTNTSRTIWRNYFKDPNLVNLIDTALKNNQELQITLQEIEIAKNDIRLKKGLLLPQV
                     GIGAGAGIEKVGRYTSQGAGDASTEIKPGKEMPDPLGDFKIGAYAHWEVDVWKKLRNS
                     KKATVSRYLSTIEGKNFVITNLIAEVANSYYELLALDSQLEIVKQNIALQSNALEIVK
                     IQKEAARATELAVQKFQSEVLASKSMEFDILQNIKETENRINFLLGQYPQEIKRDKTN
                     FLSLLPSEVKSGIPSQLLENRPDIKQAELELEAAKLDVKVARAEFYPSLDITASIGVN
                     AFKPAYLFTLPESLLYSLAGDIAAPLINRNAIKAEYSSANARQLQALYNYERTILNAY
                     LEISTELSKINNLEKSYDLKSQQVAALNRSIEVSGDLFKSARVDYFEVLMTQRDALET
                     KLELIETKKSQLNASVLVYRDLGGGWK"
     gene            complement(1557..3248)
                     /gene="ilvD"
                     /locus_tag="PLQ70_10270"
     CDS             complement(1557..3248)
                     /gene="ilvD"
                     /locus_tag="PLQ70_10270"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQE35041.1"
                     /translation="MRSDEVKKGYQRAPHRSLFRATGLTDADFDKPFIGVANSFIEII
                     PGHFFLDRVAKIIKEEIKANGCVPFEFNTIGVDDGIAMGHDGMLFSLPSRELIASSIE
                     TVMNAHKLDAMIAIPNCDKIVPGMIMGALRVNVPTVFVSGGPMKKGYTKDGTPIDLAT
                     AFEAVGKFEAGQINEAELKDIECNACPSGGSCSGMFTANSMNTLMEAMGIALPGNGTI
                     LALTKEREQLYRSAARRICEIAKDEAKSKKFKLKNILNENAVRNAFAVDMAMGGSSNT
                     VLHMLAIAKEADVNFNLEDINSISKKVSHIAKISPSLSTVHMEDINTAGGVNAVMKEM
                     TKRGDSILIDNLTISGETILEKIKDAFIKDTSIIHTIDNPYSQVGGLAILYGNLAEQG
                     AVIKTAGITGDRVFTGTAVCFDGQQEAVDGITSGKVKAGDVVVIRYEGPKGGPGMQEM
                     LAPTSLIMGMGLGSKVALITDGRFSGATRGASIGHVSPEAAEGGMIGLLKDGDEIHID
                     VDQYILSVNLSDEEIAKRKAEFVPLKKVITSKWLGQYRSLVTNASSGAVLKTDLF"
     gene            complement(3431..4237)
                     /gene="cobA"
                     /locus_tag="PLQ70_10275"
     CDS             complement(3431..4237)
                     /gene="cobA"
                     /locus_tag="PLQ70_10275"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HQE35042.1"
                     /translation="MQTNNSKLTVVGAGPGDVELITLKAIKALESADVVLYDALVNEE
                     LLHYATKATCIFVGKRLGCHAYSQDQINQLIVSQAKRFGHVVRLKGGDPFVFGRGSEE
                     IEYAQRFGIQTAIVPGISSALGVPASNGISLTQRGISESFWVITGTTSDHKLSKDVTL
                     ASKSSATVVILMGMNKLDEIISIYQNNRTDNLPIAIIQNGTKNSQKKVVGTINSITKI
                     VANQQISSPAIIVIGEVVNHISNLTKSFQEDNFLEDEFIFQNLNLTEILS"
BASE COUNT         1336 a          789 c          775 g         1452 t
ORIGIN      
        1 taaccgaaga atttgacgac aatcatgctg aataaaataa aaacatttaa atatattgtt
       61 ccgctaggta tttgcctagc ggtggcaagt tgcacaccag tccttgctcc tttggcagaa
      121 actaaagctg ttcccgaatc tttcggaaaa agtactgata caacgaatac ttctcgcaca
      181 atttggcgaa attatttcaa ggatccaaat ctggtcaatt tgattgatac tgctttgaaa
      241 aacaatcagg aattacaaat tactctacaa gaaattgaaa tcgccaagaa tgatattcgt
      301 ctaaaaaagg ggttgttatt accacaggtt ggaattggag caggagctgg aattgagaaa
      361 gtaggtcgct ataccagtca aggtgctgga gatgcatcta ccgaaataaa accaggtaaa
      421 gaaatgcctg acccacttgg tgattttaaa attggagcat acgctcattg ggaagtagat
      481 gtttggaaaa aacttcgaaa ttctaaaaaa gctaccgtga gccgctattt atcaactatc
      541 gaagggaaga atttcgtgat tacaaatctt attgccgaag tggccaattc ttattacgaa
      601 ttgttggcct tagacagtca attagaaatt gtaaaacaaa atattgcatt gcaatctaat
      661 gctttagaaa ttgttaaaat tcagaaagaa gctgctagag caactgaatt ggcagttcaa
      721 aagtttcagt ccgaggtttt ggcttctaaa agtatggagt ttgatatttt gcaaaacatc
      781 aaagaaaccg agaatagaat caactttttg ttgggtcaat atccacaaga aattaaaaga
      841 gataaaacca attttttgag tttgttgcct tcagaagtga aatctggtat tccatcgcaa
      901 ttattagaaa atcgtccgga tattaaacaa gctgaattag agttggaagc cgcaaaatta
      961 gatgtaaaag tagctcgagc cgagttttat ccttcacttg atattacagc ttcaatagga
     1021 gtaaatgctt ttaaaccagc ttatttgttt acgcttccag aatcgttgtt gtattcattg
     1081 gcgggagata tcgctgctcc tttgattaat agaaacgcca ttaaagccga atatagttcg
     1141 gccaatgcga gacaacttca agcattatac aattacgaac gtactatttt gaatgcgtat
     1201 ttagaaattt caaccgagct ttctaaaatt aataatttag aaaaaagtta tgacctaaaa
     1261 tcacaacaag tggcagcttt aaatcgttca atagaagtgt ctggcgattt attcaagtct
     1321 gctagagtcg attattttga agttttgatg acccaacgtg atgctttgga aaccaaactt
     1381 gaactaattg aaaccaaaaa atcccagctc aatgcctcgg ttcttgttta tagagatctt
     1441 ggaggtggtt ggaaataatt tcttttttgt tgctttaaaa gcttaaaaag atacaaagta
     1501 ttaaactgcc cccaaatagt ttagagcttt ggggggcagt ttttttttaa ataatcttaa
     1561 aacaaatcgg tttttaatac agcgccgcta ctagcattgg taaccaaact tctgtattgt
     1621 cccaaccatt ttgatgtaat tacttttttc aaaggaacaa actcggcttt tcttttagcg
     1681 atttcttcgt cgcttaaatt tacagaaaga atgtattgat ctacatcaat atgaatttca
     1741 tcaccgtctt ttagtaatcc aatcattcca ccttcggcag cttctgggct tacgtgtcca
     1801 atacttgctc cacgggttgc tccactaaat ctaccatcgg tgattaaggc tacttttgag
     1861 cctagtccca ttcccataat caaggaagtt ggagctagca tttcttgcat tcctggacca
     1921 ccttttggcc cttcataacg aatcacaact acatcaccag cttttacctt tccgctagta
     1981 attccgtcaa cagcttcttg ttgaccatca aaacaaactg cagttcctgt aaaaacgcgg
     2041 tctccagtaa ttccagcagt tttgataaca gcaccttgtt cggcaagatt tccatacaaa
     2101 atagctaaac ctcccacttg agaataggga ttatcgatgg tgtgaataat tgaagtatct
     2161 tttatgaaag cgtcttttat tttttctaaa attgtttctc cgcttatggt aaggttatct
     2221 ataagaatag aatctcctct tttggtcatt tctttcatta cagcatttac gccaccagca
     2281 gtattaatat cttccatgtg aacagtagaa agagatggtg aaattttagc aatatgtgac
     2341 acttttttag agatgctatt gatatcttca agattgaaat tcacatcggc ttcttttgca
     2401 atagctagca tgtgtaaaac ggtattcgaa cttccaccca ttgccatatc tactgcaaat
     2461 gcatttcgta cagcattttc gtttaagata tttttgagtt tgaatttttt agatttcgct
     2521 tcgtcttttg ctatttcgca aattcttctg gcagcacttc tatacaattg ttcacgctct
     2581 ttggtcagtg ctaaaatagt tccatttcca ggaagtgcaa ttcccatggc ttccataaga
     2641 gtattcatgg agtttgcggt aaacattcca gagcaacttc cgccacttgg acaagcattg
     2701 cactcaatgt ctttaagttc ggcttcattg atttgacctg cttcaaattt tccaaccgct
     2761 tcaaaagctg tggctaagtc aataggcgtt ccgtctttgg tatatccttt tttcattgga
     2821 ccaccgctta caaatacagt aggaacatta actcttaagg ctcccataat catacccgga
     2881 acaattttat cacagtttgg aatggcaatc atggcgtcca atttgtgtgc attcattacg
     2941 gtttcgatcg agctggctat cagttctctt gaaggtaatg aaaatagcat tccgtcatgt
     3001 cccatggcaa taccatcatc tacaccaata gtgttgaatt caaaaggaac acagccgttt
     3061 gctttgattt cttcttttat aattttggct actctgtcta aaaaaaagtg acccggaatg
     3121 atttcaataa atgaattggc cacaccaata aacggtttgt caaaatcggc atcggtcaat
     3181 cctgttgctc taaataatga tctatggggt gctctttgat accctttttt tacttcatca
     3241 cttctcatgt tgttgttttt attttttaat atatgtacga cttgtttttg ttaaaagatt
     3301 agacaatctt ctagaataac tgcaaaagta cataaaatcc ttaggggata aaataataat
     3361 tgtattcagg ttgcaaattt agaattgcaa aaagattttt ttttattgaa aggggagttt
     3421 caagtgtttt ttaggataaa atttcagtaa ggttcaaatt ttggaaaata aattcgtctt
     3481 caaggaaatt atcttcttga aaggatttcg ttaaatttga aatgtgattt accacttcgc
     3541 caataactat aatggctggg gaagaaattt gttgattagc aactattttg gttattgagt
     3601 ttatggttcc aactactttt ttctgactgt tttttgttcc gttttgaatg atggctatgg
     3661 gcaaattatc ggttcggttg ttttgataaa tggagatgat ttcgtctaat ttgttcattc
     3721 ccatcagaat aaccactgtt gccgaagatt ttgaagccaa agttacatct ttggataatt
     3781 tatggtcaga agtagtaccc gttattaccc aaaaactttc tgaaattcct ctttgtgtca
     3841 aactaattcc gttggatgca ggaacaccta aggctgatga aattcccgga acaattgccg
     3901 tttgtattcc aaaacgttga gcatattcaa tttcttcact tcctcgaccg aaaacaaaag
     3961 ggtcgccacc ttttagcctt actacgtgtc caaaacgttt ggcttgtgaa acaataagtt
     4021 gattgatttg gtcttggcta taagcgtgac aacccaatcg tttgccaaca aaaatgcatg
     4081 ttgctttggt agcgtagtgt aataattctt catttacgag tgcatcatac aagactacat
     4141 cagcactttc aagggctttg atggctttga gagtaattaa ttctacatct ccaggaccag
     4201 ctcccacaac ggttaatttt gaattattcg tttgcataat tttctcgttt aaattaagat
     4261 tattttgatg tttggtaaat aaataatttt aatatgattt aaaattaagt ataaatacgt
     4321 atgcaaatat aattaaaaaa tacgtagttt ga
//
LOCUS       DAOSNN010000223         4343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_20267_length_4343_cov_4.366838, whole genome shotgun sequence.
ACCESSION   DAOSNN010000223 DAOSNN010000000
VERSION     DAOSNN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20267_length_4343_cov_4.366838"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLQ70_10280"
     CDS             <1..333
                     /locus_tag="PLQ70_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQE35043.1"
                     /translation="STILRATEGASQSDYHELRYAITDKTGEERIIHWGDATLKHYFR
                     FEGQSHHAGEYQNYLDYFVDSIEQGFTAYPNMEEGIGTVALLQAMDKTLQTGMPVKIA
                     DVLNEYGL"
     gene            330..>1746
                     /locus_tag="PLQ70_10285"
     CDS             330..>1746
                     /locus_tag="PLQ70_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026086.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HQE35044.1"
                     /translation="MNSIYDKLTTLDFIIVAIYLVILLVIGYVVSVKQSKKDETLFMA
                     GNSLNWYNIGFNMWGTNVGPSSLLAFASIGYATGIVAGNFEWYAFVFLLLLAIVFAPR
                     YIASKVSTMPEYMGKRYGDSTQTILAWYAMVKILVSWLSLGLFSGGILVRQILGIPMW
                     QSVTVLVIFSGIFTFAGGLKAIAKVNVFQMILLIVVSLTLSYLGLEKVGGIHALIAKT
                     PANFWNLVQPANDAHYPWPAILLGYPVAAVAFFCTDQSMVQSVLGAKNLEQGQLGVNF
                     IGWLKVMALPLFILPGILCSVLFPELGKNSDLAYMTMVTNLFPSGMNGLVICVMIAVL
                     VGTIGSSLNALSTVFTNDIYVKKINPAATIKEQIKIGRITVAAGCVFAILIAIAIDNI
                     KGQNLFNIFQAVLGFLAPSLSVVFLLSIFWKRTTKKAVNATLSWGSAFSLFVGVLYLW
                     IFPADKYPVWPHFLLISFYIFV"
     assembly_gap    1747..1846
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1847..3919
                     /locus_tag="PLQ70_10290"
     CDS             <1847..3919
                     /locus_tag="PLQ70_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-rhamnosidase"
                     /protein_id="HQE35045.1"
                     /translation="DFEVWLSNKMQVRRTEREAVFPPLWQYYSPYALVTFQTEVDIPE
                     SDEVKIYSEGSFQLLLDGVQVYGQPKSIKIPAGKHKISFKVYNQEVLPAIYIAGKYVK
                     SNASWKVTNEDKLWIDETGKAQQSGTPWVPVGSWNFDSPESKPSGFKLTTTPWNAKKT
                     EKIGDGKLVDFGKETFGYIKIHGLKGKGKVALYYGESREEALDTAKCETLDHLSFDGN
                     QPETYTHNGSKAFRYVRVQADPTVKYDSISMLYEYLPLDYRGAFKSSDKQLNQIWDVS
                     AYTMHLTSREFFIDGIKRDRWVWSGDAYQSYLMNYYLFFDSASVERTLLALRGKEPVT
                     AHVNIIMDYSLYWFVGVYDYYLHTGDTKFIKTFYPRMKSLMEFCLKRRNKNGFLEPLE
                     GDWVFIDWANGLPKTGEVSFEQMLLARSLEAMAVSAKIAGENEDQKQYQKLAVDLKTK
                     LFDVFWDKKENVMKHQRIDGKMQDIVTRYANMFGIFFNYFNEEQKQSIKNKVLLNDKI
                     LKITTPYMRFYELEALCAMGEQDYVLKEMKDYWGGMLKEGATSFWEEYNPTKKGTEHL
                     AMYGRPYGKSLCHAWGSSPIYLLGKYYLGVKPTAPGYAEYEIKPNLGGLQWMEGKVPT
                     PKGEVTLYVSKSEIKVKAAEGEGKLIFESQSKPKTNSGTITELSKNKYQLTVQPNVEY
                     QISYKAVK"
     gene            3919..>4343
                     /locus_tag="PLQ70_10295"
     CDS             3919..>4343
                     /locus_tag="PLQ70_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35046.1"
                     /translation="MIQKSKHIYCLFLVFMGFSVLAQNTSWQAAPFEVVNSNYKTFKT
                     TQYAHVFSGSKHNIVRIAPELQGLTGVELPLESYKNGTNSPLKLKFKEPVQVLIGIFQ
                     DKDNADYRQLNNAKLVLENGLTITGLPPVNVYAISFSKGT"
BASE COUNT         1304 a          702 c          949 g         1288 t
ORIGIN      
        1 agcactattt tgagagctac cgaaggcgcc agccaatccg attatcacga attgcgttat
       61 gccattaccg acaaaacggg cgaagaaaga atcatacatt ggggtgatgc aaccctgaaa
      121 cattatttcc gttttgaagg acaaagtcac catgctgggg aatatcaaaa ttatttggac
      181 tattttgtgg acagcatcga acaaggtttc acggcttacc cgaatatgga agaaggcatc
      241 ggaaccgtgg cattattgca ggcaatggat aaaacattac aaaccggaat gccagtaaaa
      301 attgctgatg ttcttaacga atacggacta tgaatagtat ctacgataag ttaacgaccc
      361 tagattttat aattgttgcc atttatttgg tgatattatt ggtcattggt tatgtggtca
      421 gtgtgaaaca aagcaagaag gacgaaacgc tttttatggc gggaaattcc ttgaattggt
      481 acaacatcgg attcaatatg tgggggacaa atgttgggcc ttcgtctttg ttggcttttg
      541 ccagtattgg ttatgccacc ggaatcgtgg cgggaaattt tgaatggtat gcttttgtgt
      601 ttttattgct gttggctatc gtttttgctc caagatatat tgcgagtaaa gtcagcacga
      661 tgccagaata tatgggaaaa cgctatggcg acagtaccca aactattttg gcctggtatg
      721 ctatggtgaa aattttggtg agttggttgt cgttgggatt gtttagtggc ggaattttgg
      781 tacgccaaat tttaggaatt ccaatgtggc agagtgttac ggttttagtg attttttcag
      841 ggatttttac tttcgccgga ggattgaaag ccattgccaa agtgaatgtt tttcaaatga
      901 ttttattgat tgtcgtttcg cttacattat cttatttagg gctcgaaaaa gtgggaggca
      961 ttcacgcatt gattgcaaaa acgcctgcca atttctggaa cttggtacaa cccgccaacg
     1021 atgcccatta tccttggcca gccattttgt tgggttatcc tgttgcggca gtggctttct
     1081 tctgtaccga ccaatcgatg gtgcaaagtg tattgggagc caaaaatttg gaacaaggac
     1141 aattgggagt gaatttcatc ggctggctaa aagtgatggc gttgcctttg tttattttac
     1201 cgggaatttt atgttcggtt ttgtttccag agctagggaa aaattctgat ttggcttata
     1261 tgacgatggt tacgaattta ttcccaagcg gaatgaacgg tttggtcatt tgcgtgatga
     1321 ttgccgtttt ggtggggaca attggttctt cattgaatgc tttgagtacg gtttttacca
     1381 atgatattta tgtaaagaaa atcaatcctg cggcgactat caaggagcaa attaaaattg
     1441 gtcgtatcac cgttgcagct ggatgcgtct ttgcaattct gattgctatt gccatcgata
     1501 atatcaaagg acaaaattta ttcaacattt ttcaggcggt tttgggcttt ttggctcctt
     1561 cgttgtctgt ggtttttttg ttgagtattt tctggaaaag aactacgaag aaagccgtga
     1621 atgccacttt atcttggggt tcagccttta gtttattcgt tggagttcta tatttatgga
     1681 ttttccctgc tgataaatat ccggtttggc ctcacttttt gttgatttcc ttttacattt
     1741 ttgttannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngatt tcgaagtttg
     1861 gttaagcaat aaaatgcaag taagacgtac cgaacgcgaa gcagtatttc caccgctttg
     1921 gcaatattac agtccttatg ccttggtaac ttttcaaaca gaagtagata ttccggaatc
     1981 agacgaggta aaaatctact cggaaggatc ttttcaatta ctattagatg gtgttcaggt
     2041 ttacggacag ccaaaatcaa taaaaattcc tgctgggaaa cacaaaattt cctttaaagt
     2101 atataatcaa gaagtgttgc cggctattta tattgctggc aagtatgtga aatcgaatgc
     2161 atcttggaag gtaaccaatg aggacaaact ttggattgat gaaactggaa aagcacaaca
     2221 atccggtacg ccttgggtgc ctgttggttc gtggaatttt gattcaccag agagtaaacc
     2281 ttccgggttt aaactgacaa ccacgccctg gaatgccaaa aaaacagaaa aaataggaga
     2341 tggtaaattg gtagattttg gtaaagaaac ttttggttat attaaaattc acggtttaaa
     2401 aggaaaaggc aaagtagcat tgtattatgg tgaatctcgt gaggaagctt tggatactgc
     2461 caaatgcgaa acattagacc atttgtcttt tgatggaaac cagcctgaaa cctacaccca
     2521 taatggttcc aaggcattcc gctatgttcg agtacaagcg gatccaacgg taaaatatga
     2581 ttctatttcg atgctttatg aatatttgcc attagattat cgtggtgcat tcaaatcatc
     2641 ggacaaacaa ttgaatcaaa tttgggatgt gtcggcttac acgatgcatt taacgtcccg
     2701 cgaatttttt atagacggaa tcaaacgcga ccgttgggtt tggtctggcg acgcttatca
     2761 aagttattta atgaattatt atttattctt tgattcggct tccgtagaac gaacgctgtt
     2821 ggcactgcgt ggcaaagaac ccgtgacggc tcacgtaaat atcataatgg attattcgct
     2881 ctattggttt gtgggggttt acgattacta tttacacact ggcgatacga aatttatcaa
     2941 aactttttat ccacgaatga aatcgctgat ggaattctgc ttaaaaagaa gaaacaaaaa
     3001 cggattcctg gaaccattgg aaggcgactg ggtttttatt gattgggcaa atggattacc
     3061 aaaaactggt gaggtgagtt ttgagcaaat gcttttggcg agaagtttag aagctatggc
     3121 agttagtgcc aagattgcag gagagaatga agaccaaaaa cagtatcaaa aattggctgt
     3181 tgatttaaaa acaaaattat ttgacgtgtt ttgggacaag aaagaaaacg tgatgaagca
     3241 ccagcgtatt gatggtaaaa tgcaggatat tgtaaccaga tacgctaata tgttcggtat
     3301 ttttttcaat tatttcaatg aagaacaaaa acaaagcata aaaaataaag tgttgctcaa
     3361 tgataaaatt cttaaaatca cgacacccta tatgcgtttt tacgagttgg aagccttgtg
     3421 tgcaatgggc gaacaagatt atgtgttgaa agaaatgaaa gactattggg gaggaatgtt
     3481 aaaagaaggc gctacttctt tctgggaaga atacaatcca acgaaaaaag gaacagaaca
     3541 tttagctatg tatggacgtc cttatggaaa aagtctttgc cacgcctggg gttccagccc
     3601 gatttatttg ttggggaaat actatttggg tgtaaaacca acagctccgg gttatgctga
     3661 atatgaaatc aaaccaaacc taggtggatt gcaatggatg gaaggaaaag tgcctacgcc
     3721 aaaaggtgaa gtgaccttgt atgtaagcaa atcagaaatc aaagtaaaag ctgccgaagg
     3781 cgaaggaaaa ttgattttcg aaagtcaatc aaaaccaaaa accaactcgg gaacgattac
     3841 agaattgtct aaaaacaaat accaattgac ggttcaaccg aatgtggaat atcaaataag
     3901 ttataaagca gttaaataat gattcaaaaa agtaaacata tttattgcct attcctggtt
     3961 tttatgggct tttcagtttt ggctcaaaat accagttggc aagccgctcc ttttgaagtg
     4021 gtgaattcga attacaaaac ttttaaaacg actcaatatg ctcacgtatt ttcgggcagt
     4081 aaacataata ttgttcgaat tgctccagaa ttgcaaggat tgacaggtgt agaacttcca
     4141 ttggaaagtt ataaaaacgg aacaaattcg ccattaaaat taaaattcaa ggaaccggtt
     4201 caagtactta tcggaatttt tcaagataaa gataatgccg attaccgcca attaaacaat
     4261 gccaaattgg tgcttgaaaa cggactgacc ataacaggat tgcctcccgt aaatgtgtat
     4321 gcaatttctt tttcgaaagg aac
//
LOCUS       DAOSNN010000224         4286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_20609_length_4286_cov_4.670763, whole genome shotgun sequence.
ACCESSION   DAOSNN010000224 DAOSNN010000000
VERSION     DAOSNN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20609_length_4286_cov_4.670763"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1258
                     /locus_tag="PLQ70_10300"
     CDS             179..1258
                     /locus_tag="PLQ70_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQE35047.1"
                     /translation="MKKIIAILSISAVVLTSCGGDKKEQITKEPAIAVKVSGVSENTE
                     GGFITASGKIEAENSANLSTRMMGYVTKIHVKVGQKVGAGQLLVSINNSDLQAKKAQV
                     DASILQATAAYNNAKKDYDRFVNLFKQQSASQKELDDMTARYEMAKAGLEGAKQMRNE
                     VLAQFSYSNITAPFSGTVTNTFVKEGDMANPGMPLVSMEGATQLQVTAMVSENDISSI
                     KNGMPVKVLVKSSNDRLTGKVSEVSSSATNTGGQYLVKINLDKTDNSVLSGMFVNVQF
                     PIENKVQVAKTEMVLVPETALVKQGQLTGIYTIGTGNVAILRWLRTGKTFGNQVEVLS
                     GLSANEQYIVSAEGKLYNGVLVSIK"
     gene            1342..>4286
                     /locus_tag="PLQ70_10305"
     CDS             1342..>4286
                     /locus_tag="PLQ70_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQE35048.1"
                     /translation="MQEGISGKIAHFFINSKLTILLMVALMIIGVYSSFLIPREEEPQ
                     INVPMADVMVGYPGASPTEVESRVAKPLEKIISNIKGVEHVHTTAMNGQAMLIVQFYV
                     GQDIERSYVKLYDELAKHEDMFPKGVYKPMVKTRSIDDVPMLGLTLWSEKEDDFQLRQ
                     IAEEVTSEIEKVKDVAITKEIGGSNRELKVVLDKDKMAENGVDALGIMQMIQANNGSS
                     QSGSFVQNDQEYLVTTGQFLSNSEDVENLVVGVNKNMPVYLKQVATVQDGPSTARSYV
                     SFGYGKANEKFKTAKSEYPAVTISIGKVKGADAMKISAKIIDKVEHLKKTLIPDDVHV
                     EVTRNYGETASHKVGELLLHLGIAIIAVTILVILAMGWRGGLVVFFSVPLTFALTLFA
                     YYLLGYTLNRITLFALVFVVGIVVDDSIIIAENMHRHFKMKRLPFKQAAIYAINEVGN
                     PTILATFTVIAAILPMAFVSGMMGPYMSPMPIGASIAMLLSLFVALTVTPYLGYHLLQ
                     EKEEQEHKHEEGMETSYIYKIYNKLERPLLESNSKRRILLAVTVVLLFGSVLMFFTKS
                     VVVKMLPFDNKNEFQVVIDMPEGTTLERTTAVTKEIAQYLSTKPEVVNYQNYIGTSAP
                     ITFNGLVRHYDMRGGSNMADIQVNLLHKEDRDLQSHDIAKAMRPDIQKIAKKYGANVK
                     IIEVPPGPPVLSTLVAEIYGPDYKEQIKVANQVKTILENTSDIVDIDWMVEDNQTEYK
                     LEVDKEKAMLNGIAPQQVVGNLTYLLKEYPVSNLYDENSNDNVGIVLSLDDKDKTSLQ
                     DIQNLKIRGSQGNMIPVSDLVKVVQDTLQKTIYRKDQKRVVYVTADMAGALESPVYAI
                     LGMNEKLAKMNVPKGYKVNELYMEQPTDESDFTVKWDGEWQITLEVFRDLGVAFLVVI
                     VIIYMLIVGWFQNFKTPMVMMLAIPLSLIGIVLGHWLLNAYFTATSFIGMIALAGVMV
                     RNSVLLIDF"
BASE COUNT         1383 a          708 c         1028 g         1167 t
ORIGIN      
        1 aaggactgga aaaaaccacc gatttgttgc aggcagaaac ccaaatgttc cagaaagaac
       61 tggagttgct gcaagcggtt ttcgaatatg attttacaca ggaatatttg cagtttttga
      121 ctaaataaga aaaaagagta aagaacaaag aaaaaagata attatcaata gagacaatat
      181 gaaaaaaata atagccattc tttcgatttc cgcagttgta ttaacttctt gtggaggaga
      241 caaaaaagaa caaatcacca aggaaccagc cattgccgtg aaagtaagcg gtgtttctga
      301 aaacaccgaa ggtggattta taacagccag cggaaaaatt gaagccgaaa actcagccaa
      361 tctaagcacc cgaatgatgg gttatgtgac caaaatccac gtgaaagtgg gtcagaaagt
      421 aggagcgggg caattgttag ttagcatcaa caacagcgat ttgcaagcca aaaaagcaca
      481 agtcgatgcg agtattctac aagccacggc agcctacaac aatgccaaaa aagattacga
      541 tcgtttcgtg aatttgttca aacaacaatc ggcttcccaa aaagaattag acgatatgac
      601 ggcacgttac gaaatggcaa aagcaggatt agaaggcgcg aaacaaatgc gaaacgaagt
      661 cttggcgcaa ttttcctatt cgaatatcac ggcgcctttt tcgggaacgg taaccaatac
      721 tttcgtgaaa gaaggcgata tggcgaaccc gggaatgcct ttggtgagta tggaaggtgc
      781 aacacaattg caagttaccg caatggtttc agaaaatgat atttcgagca tcaaaaacgg
      841 aatgcccgtg aaagttttgg tcaaatcatc taacgatagg ttgacaggaa aagttagtga
      901 agtgagttca tcggctacga atactggtgg acaatatttg gtaaaaatca atttagacaa
      961 aactgataat tcagtattat caggaatgtt tgtcaacgtg cagtttccta ttgaaaacaa
     1021 agttcaagtt gccaaaactg aaatggtatt ggtgccagaa accgctttgg tcaaacaagg
     1081 acaactaacg ggaatttata cgataggaac agggaatgtc gccattttgc gatggttgcg
     1141 aacaggaaaa acttttggca atcaagtgga agtcttatcg ggattgtcgg ccaacgagca
     1201 atacattgtt tcggctgaag ggaaattgta taacggagtt ttggtgtcaa ttaaataatt
     1261 tataaatgtg aaaatgtaac aattaattga aattcgattt cattgttaca ttgatatatt
     1321 aacacattaa aacattagac tatgcaagaa ggtatttcag gtaaaatagc ccattttttc
     1381 atcaattcga aattgaccat tttgttgatg gtggccttga tgataattgg cgtgtacagt
     1441 tcgttcttga ttccaaggga agaagaaccg cagattaatg ttccgatggc cgacgtaatg
     1501 gtggggtatc cgggagcgag tccaacggaa gtcgaaagcc gtgtggcgaa accattggag
     1561 aaaattattt cgaacatcaa aggtgtcgag cacgtgcaca cgacagcaat gaatgggcag
     1621 gcgatgctga ttgtccaatt ttatgtaggt caagacatcg agcgttccta cgtgaaattg
     1681 tatgacgaat tggcgaaaca cgaagatatg tttccgaaag gcgtttacaa accaatggtg
     1741 aaaacccgtt cgattgacga tgtaccgatg ttgggattga cgctttggag tgaaaaagaa
     1801 gatgatttcc agttgcgcca gattgcggaa gaagtaactt cggagattga gaaagtgaaa
     1861 gatgtggcga ttaccaaaga aattggtggc agcaatcgcg aactgaaagt cgttttggat
     1921 aaagataaaa tggccgaaaa tggtgtggat gctttgggaa ttatgcaaat gattcaagcc
     1981 aataacggta gttcacaatc aggtagtttt gtgcaaaatg accaagaata tttggtgacc
     2041 actggacaat ttttatcgaa ttcggaagat gtggaaaact tggttgttgg cgtgaacaaa
     2101 aatatgccgg tttatttaaa acaagtggcg acggtacaag acgggccatc aacagccaga
     2161 agttatgtgt cttttggcta tggcaaagcc aatgaaaaat tcaaaactgc caaatcagaa
     2221 tatccagccg ttacgatttc aatcggaaaa gtaaaaggtg cggatgcgat gaaaatttca
     2281 gctaaaatta ttgataaagt tgagcattta aagaaaactt taattccaga tgatgttcac
     2341 gttgaagtga ctagaaatta tggtgaaacg gcttcgcaca aagtaggcga gttgttgctg
     2401 cacttaggta ttgcgattat tgccgttacg attttggtga ttttggcaat gggttggaga
     2461 ggcggattgg tcgtgttttt ctctgttccg ttgacgtttg ccttgacctt gtttgcctat
     2521 tatttattgg gttatacctt gaatagaatt acgctttttg ccttggtttt cgtggtggga
     2581 atcgtggtcg atgacagtat tatcatcgcc gaaaatatgc accgccattt caagatgaag
     2641 cgactgccct ttaaacaagc agcaatttat gcgataaatg aagtaggaaa tccaacgatt
     2701 ttagcgacgt ttacggttat tgcggctatt ctgccaatgg cgttcgtatc tggaatgatg
     2761 ggaccttata tgagtccgat gccgattggt gcttcgattg cgatgttgtt gtctttgttt
     2821 gtggccttga ccgtgacgcc ttatttgggg tatcatttgt tgcaggaaaa agaggagcaa
     2881 gagcacaaac acgaagaggg aatggaaacc agctatattt ataaaatcta taataaacta
     2941 gaacgcccat tattggaaag taactctaaa agaagaattc ttttggctgt aactgtggta
     3001 ttattgttcg gttcggtttt gatgtttttt accaaatcag tagtcgtgaa aatgttgcct
     3061 ttcgacaaca aaaacgaatt tcaagtggtg attgatatgc ccgaaggaac aacattggaa
     3121 cgcacgacag ccgtgaccaa ggaaattgcg caatatctat cgaccaaacc cgaagtggtg
     3181 aattaccaaa attacattgg tacatctgca ccgataactt tcaacggatt agtgcgtcat
     3241 tacgatatgc gtggtggcag caatatggcg gatattcagg tgaacttgtt gcataaggaa
     3301 gaccgtgatt tacaaagtca cgatatcgcc aaagctatgc gtcccgatat ccaaaagatt
     3361 gccaagaagt atggtgcgaa tgtgaaaatt attgaagttc cgccaggacc gccggtttta
     3421 tcgactttgg ttgccgagat ttatggacca gattataagg aacaaatcaa agtggcgaat
     3481 caggtgaaaa ccattttgga aaacacttcg gatattgtcg atatcgattg gatggtagag
     3541 gacaaccaaa cggaatacaa actggaagtc gataaagaaa aagcaatgct taacggaatt
     3601 gctccacaac aagtggttgg gaatttgacg tatttgttga aagaatatcc cgtttctaat
     3661 ttgtacgacg aaaattccaa tgacaatgtt ggaatagttc tttctctgga cgacaaagac
     3721 aaaacgagtt tgcaggacat tcaaaacctg aaaataagag gcagccaggg gaatatgatt
     3781 ccggtgagtg atttggtgaa agtggttcaa gataccttgc agaaaaccat ttacagaaaa
     3841 gatcaaaaac gcgtggttta tgtaacggct gatatggctg gagctttgga aagtcctgtg
     3901 tatgcgattt tgggaatgaa tgaaaaacta gccaaaatga atgtcccaaa aggctataaa
     3961 gtcaacgaat tgtacatgga acaaccaacg gacgaaagcg attttaccgt aaaatgggat
     4021 ggtgaatggc aaattacatt ggaagttttc cgtgatttgg gtgtcgcatt tctggtagtg
     4081 atcgtgatta tttatatgct gattgtgggt tggttccaga acttcaaaac gccaatggta
     4141 atgatgttgg cgattccgct ttcactcatt ggaattgtgt tggggcactg gttgttgaat
     4201 gcttatttca cagcaacttc ttttattgga atgattgctt tggcaggcgt tatggttcga
     4261 aattcggttt tacttattga ctttat
//
LOCUS       DAOSNN010000225         4279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_20657_length_4279_cov_4.236742, whole genome shotgun sequence.
ACCESSION   DAOSNN010000225 DAOSNN010000000
VERSION     DAOSNN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20657_length_4279_cov_4.236742"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1946
                     /locus_tag="PLQ70_10310"
     CDS             <1..1946
                     /locus_tag="PLQ70_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HQE35049.1"
                     /translation="TITGFLQDDETRILGELSLHHNHALEDLQKNAWIKQIKILKDAL
                     QNFDTGQIYFEFSIPRMGKRVDNVLIINDLIFVLEFKVGDKEYQKHSIEQVVDYCLDL
                     KNFHEGSHHEKLIPILVSTKAEAIENIFEVTDNLFEPLKANEQNLSEVISNSLLQSNG
                     RQINSTDWENSIYKPTPTIIEAAQALYKGHNVKEISRHDAGAINLSKTTDCINNIIET
                     AKRENKKSICFLTGVPGAGKTLAGLNIANERMKADEDEHAVFLSGNGPLVDVLREALT
                     RDEVATSKDNGEPLTKKVAAIKANAFIQNIHHFRDDNLISKKAPVEKVVVFDEAQRAW
                     AKEQVSSFMKRKKGVEDFDMSEPEFLISVMDRHEDWCTIICLIGGGQEINTGEAGVYG
                     WLSSLKNNYPHWDIHYSNLISSDDNYLSDLELRNWVQSVATEKHELHLSVSVRSFRSE
                     KISELMHEILDAKNERAKTLIGEVKNDFPIFITRDLSTAKKWLRTQAKGSERIGLVAS
                     SGGRRLRPLGIDVKNEISAPNWFLNNSDDIRSSYFLEEIATEFDIQGLEIDWVCLAWD
                     INYYFENGKWNCQYFSGTKWQNIHNENDKTYLQNAYRVLLTRARQGLIIYIPDGDDLD
                     HTRPKVLYDTTFNYFKSCGIQTI"
     gene            2100..2339
                     /locus_tag="PLQ70_10315"
     CDS             2100..2339
                     /locus_tag="PLQ70_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35050.1"
                     /translation="MTQTDLIGFIGVTILLVAFFLNLNNTISKDSKTYLLLNFIGAGL
                     ACLASVLMKYLPFIILEGSWTLVSALGLLKLMKQK"
     gene            complement(2515..3171)
                     /gene="fsa"
                     /locus_tag="PLQ70_10320"
     CDS             complement(2515..3171)
                     /gene="fsa"
                     /locus_tag="PLQ70_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQE35051.1"
                     /translation="MKFFIDTANLKDIKEANDLGILDGVTTNPSLMAKEGITGADNIL
                     AHYVKICELVDGDVSAEVIATDFEGMVAEGEMLAALHPQIVVKLPMIKDGVKACKYFS
                     NKGIRTNVTLVFSAGQALLAAKAGATYVSPFIGRLDDISTDGMALIEEIRLIYDNYGY
                     ETQILAASVRNVMHIINCAKIGSDVITGPLSAIEGLLKHPLTDIGLATFLADFKKGNS
                     "
     gene            complement(3198..>4279)
                     /locus_tag="PLQ70_10325"
     CDS             complement(3198..>4279)
                     /locus_tag="PLQ70_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQE35052.1"
                     /translation="TANPALAQKMEMFLSGKLPDLDLSQVVQKANVATREASSIVLGY
                     LAENLENVIVSSADLSNSDKTDGFLKKTTVLQKNNFKGAFLQAGVAELTMTAIANGIA
                     LHGGVVPVVATFFVFSDYMKPAIRLAAIQELPVKYVLTHDSFRVGEDGPTHQPIEQEA
                     QMRLMEKIKNHSGHQSLLALRPADAIETSVAWDMALKNTTTPTALILSRQNIKDIPAM
                     GTSRYQEATEANKGGYLVKSVANPDITLFANGSEVATLMDAAVILEKENNLKVNVASI
                     ISEGLFKQQSAAYQESVIPKGKLVFGLTAGLPVNLEGLIGDSGKVIGLGHFGYSAPAS
                     VLDQKFGFNPESASKEILKYIQESK"
BASE COUNT         1449 a          754 c          759 g         1317 t
ORIGIN      
        1 aaaccataac cggcttcttg caggacgatg aaacaagaat tcttggagag ttgagtttac
       61 accacaatca tgcacttgag gacttacaga aaaacgcatg gattaagcaa atcaaaattc
      121 tcaaagacgc tctgcaaaac tttgacaccg gacaaatcta ttttgagttt tcaatcccaa
      181 gaatgggaaa acgggttgac aatgtattaa tcattaacga cctaattttt gttttagaat
      241 ttaaagttgg tgacaaggaa tatcaaaaac attctattga gcaagtcgtt gactattgct
      301 tggacttgaa aaactttcac gaaggaagtc atcatgaaaa attaattccg attttggttt
      361 caacaaaagc cgaggcgata gaaaatattt ttgaggtgac agacaattta tttgaaccgc
      421 ttaaagctaa cgaacaaaat ctaagtgaag taatttccaa ttcactttta caaagtaacg
      481 gcagacaaat aaattcaacc gattgggaga actcaatata caaacctact cctactatta
      541 tagaagctgc acaagcactt tacaagggac ataatgtaaa agaaatttca agacatgatg
      601 ccggtgctat aaatctttcc aagacaacgg actgcattaa caacattatt gagacagcaa
      661 aaagagaaaa taaaaaatca atttgctttt tgaccggagt tcctggtgca ggaaaaacgc
      721 tagctggatt aaatatagcg aacgaaagaa tgaaagcaga tgaagacgaa catgcagttt
      781 ttctatcagg aaacggacct ttagtagatg tattgagaga agccttaacg agagatgaag
      841 ttgcaacctc aaaagacaat ggtgaaccac ttacaaaaaa agttgctgca ataaaagcta
      901 acgcttttat tcaaaatatt catcactttc gagatgataa tttaatttca aagaaagccc
      961 cagttgaaaa agttgtagta tttgatgaag cacaaagagc atgggcaaaa gaacaggtaa
     1021 gctcttttat gaaaagaaaa aaaggagttg aagattttga catgtcagag cccgaattct
     1081 taatcagtgt tatggataga catgaagatt ggtgcacaat catctgttta ataggtggtg
     1141 gacaagaaat taatactggc gaggcaggag tttatggatg gctttcctct ttaaaaaaca
     1201 actatcctca ttgggatatt cattattcga atttgatttc ttcagatgat aattatttat
     1261 cagacttaga attacgaaat tgggttcaat ctgtggctac tgaaaaacat gaattacatt
     1321 tatcagtttc tgtaaggtct tttcgttcag aaaaaatttc cgagttaatg catgaaattt
     1381 tagacgctaa aaatgaaaga gcaaaaacac ttataggaga agtcaaaaat gattttccaa
     1441 tttttattac aagagatttg tcaacagcaa aaaaatggct gcgaactcaa gcaaaaggaa
     1501 gtgaaagaat tggacttgtt gcaagttcag gtggcagaag attaagacct ttaggaattg
     1561 acgtcaaaaa tgaaatttct gctccaaatt ggttcttaaa taattcagat gatattcgtt
     1621 cttcttattt tttagaagaa atagctactg aatttgacat acaaggttta gaaattgatt
     1681 gggtttgttt agcctgggac atcaactatt attttgaaaa cggaaaatgg aattgtcaat
     1741 atttttcagg gactaaatgg caaaatattc ataacgaaaa cgataaaaca tatttgcaaa
     1801 acgcttacag agttttacta accagagcaa gacaaggatt aataatttat attcctgatg
     1861 gtgacgacct tgaccatact agaccgaaag tactttatga cacgacattt aactacttta
     1921 aatcttgtgg aattcaaact atataacgac taaaaaagaa gtacgaaccg gtaacaagcg
     1981 tttggcaaag tggcggttca gtgttacgca gacacatttg tggtgaaaat cgccaccttc
     2041 gccaagcgcc aaaacattgc gcttcagttt accacaacta aaactaaaat caaaatttta
     2101 tgactcaaac agatttaatt ggatttattg gagtaacgat attgttagtc gctttctttt
     2161 tgaacttaaa caataccatt tctaaagaca gtaaaaccta tttgttgcta aattttattg
     2221 gagcaggatt ggcgtgtttg gcatctgtgt taatgaaata tcttcctttt ataattttgg
     2281 aaggaagttg gactcttgtt tctgctcttg gattattaaa attgatgaaa caaaaatgaa
     2341 ctctactcca tttgtcttgc cagaagcgtt ctacttacaa atgcacataa ttgagacgct
     2401 tacagcgtga caaatagcaa gataaaaaag cacaaaacag ataatacaaa caccaatcaa
     2461 aacaaaaaaa ccgcctcaat gagacggttt ctttagtttt aaactattta ttttctagct
     2521 atttcccttt ttgaaatcag ctaagaaagt agccaaacca atatcggtca atgggtgttt
     2581 caacaaacct tcgatagcgc tcaaaggacc tgtgattaca tccgatccaa ttttggcaca
     2641 attgataatg tgcattacat tacgaactga tgcagctaga atttgagttt cgtacccgta
     2701 attatcataa atcaatctga tttcttcaat taaagccatt ccatctgtag aaatatcatc
     2761 caatcttccg atgaaaggag acacataagt tgctccagct tttgcagcca ataacgcttg
     2821 accagcagaa aacactaaag taacattggt tctaattcct ttgtttgaaa aatatttaca
     2881 tgctttaact ccgtctttaa tcatcggtag tttaaccacg atttgaggat gcaaagcagc
     2941 aagcatttcg ccttcggcaa ccattccttc aaaatctgta gcgattactt cggcgcttac
     3001 atcaccatct accaattcac atatttttac ataatgagca aggatattat cggctcctgt
     3061 aattccttct ttcgccatta gcgatggatt agtcgttaca ccgtctaaaa ttccaagatc
     3121 attggcttct tttatgtctt ttaaattggc agtatctata aaaaatttca tttctttttg
     3181 ggtttatttt tgattaatta tttactttct tgaatgtatt ttaggatttc tttgctagca
     3241 ctttcagggt tgaaaccgaa tttttggtct aaaacgcttg ctggtgctga ataaccaaaa
     3301 tgacccaatc cgatcacttt tccgctatcg ccaattaacc cttcaaggtt tacaggaagt
     3361 cccgcagtaa ggccgaaaac taattttcct ttaggaatta cgctttcttg gtaagctgca
     3421 gattgttgtt tgaacaaacc ttctgagata atagaagcta catttacttt taggttattc
     3481 tctttctcca aaataacagc agcatccata agtgttgcaa cttcagaacc gttagcgaat
     3541 aaagtaatat caggatttgc aactgatttt actaaatatc ctcctttgtt agcttcggta
     3601 gcctcttggt atcttgaagt tcccatagct ggaatgtctt tgatattttg tcttgaaaga
     3661 atcaaagccg taggagttgt tgtgtttttc aacgccatat cccaagcaac agaagtttcg
     3721 atagcatcgg caggacgaag tgctagtaaa ctttggtgac ctgaatggtt tttgattttt
     3781 tccatcaaac gcatttgagc ttcttgctca attggttgat gtgttggtcc gtcctcacct
     3841 actctaaatg aatcgtgagt caagacatat tttactggca attcttgaat agcagccaat
     3901 cgaatagcgg gtttcatata atctgaaaac acaaagaaag tagctacaac tggaacaaca
     3961 cctccgtgaa gggcaatacc atttgcaata gcagtcattg ttaattcagc aactccggct
     4021 tgtagaaaag ctcctttgaa gttgtttttt tgcaaaacag tagttttttt caagaaacca
     4081 tctgttttat cactgttaga taaatctgct gaagaaacaa ttacgttttc aagattttcg
     4141 gccaaatatc ctaagactat agaagaagct tctctagtag ccacattcgc tttttgaacc
     4201 acttggctta aatctaaatc aggtaatttt ccagaaagga acatttccat tttttgagct
     4261 aaagctggat tagctgttt
//
LOCUS       DAOSNN010000226         4270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_20714_length_4270_cov_5.578410, whole genome shotgun sequence.
ACCESSION   DAOSNN010000226 DAOSNN010000000
VERSION     DAOSNN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20714_length_4270_cov_5.578410"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..929
                     /locus_tag="PLQ70_10330"
     CDS             258..929
                     /locus_tag="PLQ70_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQE35053.1"
                     /translation="MRSHILEVDSIRKHFDYKLVISDVYLKCVSNDIIALLGRNGSGK
                     STLLRIIFGIVSAENKYIRIDGIVKNKTASLLDEISYLHQESFIPSHLSVKKAIFLSI
                     SFEKRICFLEDSLIQSILSKKISYLSSGELRYLEIKLVLFNSSKFVLLDEPYNGLSPI
                     MIEKVNQLIKDNSLKKGIVITDHNYENVIRISTQLLLMKDGKIHAIKDKNELVEKGYL
                     SLRMT"
     gene            complement(926..1708)
                     /locus_tag="PLQ70_10335"
     CDS             complement(926..1708)
                     /locus_tag="PLQ70_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQE35054.1"
                     /translation="MNQILYKNTKISYSDTGKGNAVVLLHGFLENQTMWQDLVPELSK
                     KYRIITIDLLGHGETECLGYVHSMEDNADVVQAVLSKLRIRKAVFVGHSMGGYVALAF
                     AELYPENVRGLVLMNSTSKADSDERKANRDRAIKAVKKDYTTFIRLSIGNLFSPDNRE
                     RLIDEIEKVKIEALKTPLQGVVASLEGMKIRKDREVLLHLTPYPKMLILGEKDPVLNY
                     EDALEQIENTTVKLITLPDGHMSHIENREELKKVLLDFFKGV"
     gene            complement(1879..3168)
                     /locus_tag="PLQ70_10340"
     CDS             complement(1879..3168)
                     /locus_tag="PLQ70_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HQE35055.1"
                     /translation="MKYHPIDRQLFIKNRAKFTTQMKPNSVAIFNSNDIYPVSADSSL
                     PFAQHRDIFYLSGVDQEESILLLFPDAPYENQREMLFLKETSEHIAIWEGEKLTKDRA
                     FEVSGIKTVHWLQDFEKVLFELMTYSDTIYINTNEHYRATIETETREARFVKWWKEKY
                     PAHTVAKSNPILQRLRSVKESEELDLIQNACNITEKGFRRLLPFVKPNVMEYEIEAEL
                     IHEFIRNRSKGFAYTPIIASGNNANVLHYIENNQQCKAGDLILLDVAAEYANYSSDLT
                     RTIPVSGRYSDRQKAVYNAVLRVKNEATKMLTPGTLWKQYHLEVGKIMTSELLGLGLL
                     DKADVQNENPDWPAYKKYFMHGTSHHMGLDTHDYGKLTEPMQANMVFTVEPGIYIPAE
                     GFGIRLEDNVVIQKTGEPFNLMRNIPIEIEEIESLMN"
     gene            3532..4200
                     /locus_tag="PLQ70_10345"
     CDS             3532..4200
                     /locus_tag="PLQ70_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HQE35056.1"
                     /translation="MAKSAILKSSIAKKVAMALSGLFLIMFLSLHFFINMVSVFSADS
                     FNAMSHFMGYNPLIQFVMQPVLIAGVVFHFVMGFVLELKNRSARPVAYVKYDGAANAS
                     WASRNMIISGVVVLAFLGLHMYDFWVQEIAYKYCLANPIDETRYYGELVHKFESPVRT
                     GLYSVAFVLLGLHLWHGFTSSLQSVGFDNKIGKSLHKICYAFAVIVPFGFIFIALFHH
                     FINN"
BASE COUNT         1285 a          671 c          768 g         1546 t
ORIGIN      
        1 ttcgagtttt tcaaaagcac tcttttgatt aatttatctg tttgtttagt ttccttattc
       61 tttggaacta tagatacttt cttttttgtt tttgccagtt acggttttgt gataacaata
      121 ctctataaag aaatttacaa aaaaaacgat tatttatttt attacaataa tggagtttca
      181 aaattacaat tgattggttt tagttatttt tttacaatct ttttgtcagt tttattttta
      241 atactaaagg aaatattttg agaagtcata tattagaagt ggatagtatt cgaaaacatt
      301 ttgattataa attagtgatt tcagacgtgt atctgaaatg tgtcagtaat gatattatag
      361 ccttgttggg aagaaatggt tcaggaaaat ctactttgtt aaggattatt tttggaatag
      421 tttctgctga aaataaatac attcgtattg atgggattgt aaaaaataaa acagcttcat
      481 tacttgatga aattagttac ttgcaccaag agtcatttat acccagtcat ttatctgtta
      541 agaaagctat ttttttgtcg atttcttttg aaaaaaggat ttgttttcta gaagatagtc
      601 taattcagtc aattttaagt aaaaaaataa gctatttgtc aagtggagaa ttacggtatt
      661 tagaaatcaa gttagtgctt tttaattcat ccaaatttgt tctactagat gaaccctaca
      721 atggattatc tcctattatg attgaaaaag tcaatcaatt aataaaagat aattcattaa
      781 agaaaggaat tgtcatcaca gatcataatt atgaaaacgt gattcgtatt tcgacccaat
      841 tattactgat gaaggatggt aaaattcacg ccattaaaga taaaaatgaa ttggtagaaa
      901 aaggatattt aagtttgaga atgacttaaa ctcctttaaa aaaatccaac aacactttct
      961 ttaattcttc ccgattttct atatgactca tatgtccgtc gggcaaagtg attaatttga
     1021 cggtagtatt ttctatttgt tctaaagcat cttcataatt caaaacgggg tctttttctc
     1081 ccagaatcag catttttgga taaggtgtga ggtgcaataa aacttctcgg tctttgcgga
     1141 ttttcattcc ttcgagtgag gcgactactc cttgtaacgg tgttttcaaa gcttcaattt
     1201 ttactttttc gatttcgtca attaatcgtt cgcgattgtc gggactaaaa agattgccta
     1261 tagaaagtct aataaatgtg gtataatcct ttttaaccgc tttgatggcg cgatctcgat
     1321 tggcttttcg ttcgtcgctg tcggctttgg aagtggagtt cattaaaacc aaacctctca
     1381 cattttcagg gtacaattcg gcgaaagcca gagccacgta accgcccata gagtgcccta
     1441 caaaaactgc ttttcggatg cgtaatttgg acaaaactgc ttgaacaaca tcggcgttgt
     1501 cttccataga atgaacgtaa cccaaacatt ccgtttcgcc gtgacccaac aaatctattg
     1561 ttatgattcg atattttttg ctcaattctg gaactaaatc ctgccacatt gtttggtttt
     1621 ccaaaaaacc gtgaagcaac acaacggcat tgccttttcc ggtatcggaa taggagattt
     1681 tcgtgttttt gtaaaggatt tgattcatcg gtgtaaactt gaaacaaaaa taagattaaa
     1741 atttttaaac catataagaa atttaaggtc atataagaaa atcttaaatg ttcttaaatg
     1801 ccttatatgg taaataaaaa acggctcaca ttgctgcaaa ccgttttaga tattaaatct
     1861 aaattctgaa atctaaaatt aattcattaa gctctcaatt tcctcaattt caatcggaat
     1921 attgcgcatt aaattaaaag gttctccagt tttttgaatt accacattat cttctaatct
     1981 aattccgaaa ccttcggcag gaatatagat tccgggttct acggtgaaaa ccatattggc
     2041 ttgcatgggt tcggtaagtt ttccgtaatc gtgggtgtct agtcccatat gatgagaagt
     2101 tccgtgcata aaatattttt tgtaggctgg ccaatccggg ttttcgtttt gcacatcggc
     2161 tttgtccaaa agtcccaaac caagcaattc cgaagtcatt attttaccta cttcaagatg
     2221 gtattgtttc caaagtgttc ctggtgtcaa cattttggta gcttcgtttt ttactcttaa
     2281 aacggcattg taaaccgctt tttgtctgtc ggaataacgt ccggaaacgg gaatggttct
     2341 ggttaaatca ctcgaataat tggcatattc ggcggcaacg tccaataaaa tcaaatcgcc
     2401 tgccttgcat tgttggttgt tttcgatgta atgcaagaca ttagcattgt ttcccgaagc
     2461 gataatgggc gtgtaggcaa aacctttgga acgattgcga atgaattcgt gaatcaattc
     2521 ggcctcgatt tcgtattcca ttacgttggg ttttacaaac ggaagcaatc ttctgaaacc
     2581 tttttcggta atgttgcagg cattttggat taaatccaat tcttcacttt ccttaacaga
     2641 acgcaaacgt tgcaaaatcg gattgctttt ggcaacagtg tgtgcaggat atttctcttt
     2701 ccaccatttt acaaaacggg cttcacgagt ttcggtttcg attgtggcgc gataatgttc
     2761 gttggtgttg atgtaaatcg tatcggaata ggtcattagt tcgaacaaaa ctttttcgaa
     2821 atcttgcaac caatgcacgg ttttgattcc cgaaacttca aaagctcggt ctttggtcag
     2881 tttttcgcct tcccaaatcg caatgtgttc gctggtttct ttcaggaaca acatttctct
     2941 ttggttttca taaggagcat cgggaaaaag caacaaaatg ctttcttctt ggtcaacgcc
     3001 agacaaatag aaaatatctc ggtgttgtgc aaaaggtaaa ctgctgtcgg cagaaacagg
     3061 ataaatatcg ttggagttga aaatggccac gctgttaggt ttcatttgag tagtgaattt
     3121 ggcgcggttt tttataaaaa gttgtcggtc gattgggtgg tatttcataa caattcagat
     3181 ttttattttg aatactcaaa agtagaaact ttgtgaccaa taataaaaaa aataacattt
     3241 ttattttagt ttgtttcaaa atttgaattg caactatttc tattgtattt tctgaaatgc
     3301 cgtttttata tttttcttga aaaacaagta aaaacacgta ttgatttttg tttatacgta
     3361 aaaatactta ttttttaaaa atatataata atatttgatg gtatttgaga gtaaagttta
     3421 ttattaatta aacactttag acctaatata aattagttga aaaaggttgt agggtttttc
     3481 aaataaccct atttttgtga aactttttaa aaaacaattt attttatact tatggcaaaa
     3541 tctgcaatat tgaagtcgtc aattgctaaa aaagtggcaa tggcgctttc aggacttttt
     3601 ttgattatgt ttctatcgct tcactttttt attaatatgg tttcggtttt tagtgccgat
     3661 tcatttaatg cgatgtcaca ttttatgggt tacaatccgt taatccaatt tgtaatgcaa
     3721 cccgttttaa ttgctggtgt tgtttttcat ttcgttatgg ggtttgtttt ggaattaaaa
     3781 aaccgaagcg ctagacctgt tgcctatgtg aaatatgatg gtgcagcaaa tgcttcttgg
     3841 gcttccagaa acatgattat ttctggagtt gtggtgttgg cttttttagg tttgcatatg
     3901 tatgactttt gggtacaaga aattgcctat aagtattgtt tagcaaatcc aatagacgaa
     3961 actcgttatt atggagaatt ggttcataaa tttgaaagtc cagttcgtac aggattgtat
     4021 agtgtggctt tcgtgttgtt aggtttacac ctttggcacg gtttcacttc ttctttgcaa
     4081 tcggttggat tcgacaacaa aatagggaag tcattgcata aaatctgtta tgcgtttgcg
     4141 gtaatagttc cttttggatt tattttcatt gcattatttc accattttat taataattaa
     4201 tatcaatcta tcataatgaa actagattct aaaataccag aaggtcacat ttcacaaaaa
     4261 tggactgatt
//
LOCUS       DAOSNN010000227         4202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_21194_length_4202_cov_5.049916, whole genome shotgun sequence.
ACCESSION   DAOSNN010000227 DAOSNN010000000
VERSION     DAOSNN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21194_length_4202_cov_5.049916"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..993
                     /locus_tag="PLQ70_10350"
     CDS             46..993
                     /locus_tag="PLQ70_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HQE35057.1"
                     /translation="MKKIILSAAVVLMLFSCQNESTEPTTSAITHRLCASHDVLQEQL
                     EADPTLAIRMNEIEAFTQNRISKSNFRLVNGKVVIPVVVNVLYRTASENISDAQIQSQ
                     IDVLNQDFTATNTDFGSVPAEWSEIAANVGITFELVTINRKSTSKTSWGTRDAMKSTK
                     KGGLDPTSPTTTLNLWACTIGGGILGYAQFPGGSSSTDGVVIDSKYFGLSGTASYPYN
                     LGRTGTHEVGHWMNLRHIWGDASCGNDLVADTPVAKTSNFGVPAYPYVSTCLPAHNEM
                     TMNYMDYTDDRGMYMFTNGQKARMAAIFVSGGPRAAIGQ"
     gene            1072..2163
                     /locus_tag="PLQ70_10355"
     CDS             1072..2163
                     /locus_tag="PLQ70_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQE35058.1"
                     /translation="MITSKIIANGILRAIAILVFTALSFYFLYLIQSVLIYLVVALIL
                     TLIGNPILDFFKKKLKFKHTLATIATVSIFIMLIAGFILMFIPLITSQGENLSLLKSA
                     EIERNITQLKTQTFTFLENHNIDTSRMMKEANLASKINLNFIPNFLNSILGTISSFGI
                     GLASVLFITFFFLKDRLMFIIGAKKIIPDTHEEKILNSAHKINHLLSRYFIGLLLQLF
                     IVFILYIIVLFIFDIPNLFIIAFLCAVLNIVPYIGPLIASILAAILTMLSNLGSDFQT
                     VILPTTIYVLIGFWIVQIIDNNLSQPLIFSKSVSSHPLEIFLVILIAGFLSGILGMII
                     AVPLYTILKVIGKEFFPENKVIQLFTKNI"
     gene            2166..3350
                     /locus_tag="PLQ70_10360"
     CDS             2166..3350
                     /locus_tag="PLQ70_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQE35059.1"
                     /translation="MDLKILNPEIQQFINNNLDKNIAQLALQKNPFPEIEWITILNQI
                     EAKTKSKDKLPHWFTAKNIIYPSKISVEQTSSEKTASYKSELVSGESLIDLTGGFGVD
                     DFYFAKKIKEVTHCEINPELSQIVQHNFEQLNVKNIDCQSGDSLEILAKSNSKYDWIY
                     IDPSRRNDTKGKVFMLKDCLPNVPEHLDFLFNSSDKILIKTAPLLDISAGLSELKNVK
                     SIHIVALENEVKELLWELHRDYFGKTNLKTVNIVKKNRETFEFVLDENSIIPEYGLPE
                     KYLYEPNSAIMKSGGFDEVSTFYKIKKLHKHSHLYTNSEIILFPGRIFEIENTIPYHK
                     NDMKIFLAGKQANITTRNFPETVESIRKKWKIKEGGHHYCFFTTDENDNKIVLICKKI
                     TS"
     gene            3350..3916
                     /locus_tag="PLQ70_10365"
     CDS             3350..3916
                     /locus_tag="PLQ70_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35060.1"
                     /translation="MKRVSLLFFLLLSSYLFAQKTCEYSADVKDSIGTYKLTKEYLIY
                     EKNFAGNNSYIFLSLSLTDGTPTLNIQMIQKSKEFMKANCFDKNSKLFFQLNNGKIVT
                     LLHIDQENCGSLVRDGNGFDNRLLVGNFMFIKGTMEDLKSSPVNLMRIKYLTDIEDYV
                     LRKEITSELDTKVYEPENYFLNYLHCIQ"
     gene            complement(3917..>4202)
                     /locus_tag="PLQ70_10370"
     CDS             complement(3917..>4202)
                     /locus_tag="PLQ70_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQE35061.1"
                     /translation="DPAKGSIHNRGGAVDITLVDANGKALDMGTSFDFFGIEASHNYQ
                     NLSQEIKNNRELLKTTMIQNGFNPLDSEWWHYNLKTASNDKVSNAKWKCD"
BASE COUNT         1425 a          692 c          641 g         1444 t
ORIGIN      
        1 ttgttgaaaa ttaacttaaa ccactaatta tataacactt ttattatgaa aaaaattatc
       61 ttatcggcag cagttgtact gatgcttttt tcttgtcaaa acgaatcaac tgaaccaaca
      121 actagtgcta ttactcatcg cctttgtgct tcacacgatg ttcttcagga gcaattagaa
      181 gccgatccaa ctttagccat tagaatgaac gaaattgaag cttttacgca aaatagaatt
      241 tcaaaatcaa attttagatt agttaatggt aaagttgtaa ttccagttgt agtaaatgtt
      301 ttgtaccgaa ctgcttccga aaatatttct gatgctcaaa tacaatctca aattgatgta
      361 ttaaatcaag attttactgc tacaaacaca gattttggta gtgttcctgc agaatggtca
      421 gaaatagcgg caaatgttgg aattactttt gagttagtaa ctatcaacag aaaatctaca
      481 tctaaaactt cttggggaac gagagatgct atgaaaagta ctaaaaaagg aggtttagac
      541 cctacttcac caacaacgac tcttaatctt tgggcttgta ctattggtgg tggaattttg
      601 ggttatgcac aatttcctgg tggttcatct tctactgacg gagttgtgat tgattcaaaa
      661 tattttggtc tttctggcac agcaagttat ccttataatt taggtagaac aggaactcat
      721 gaagtgggtc actggatgaa tttacgtcat atttggggag atgcttcttg cggaaatgac
      781 ttagttgccg atactccagt tgctaaaact tcaaactttg gagtacctgc atatccttat
      841 gtaagcactt gtttgccagc acacaacgaa atgacaatga attatatgga ttatactgac
      901 gatagaggaa tgtatatgtt tacaaacggt caaaaagcaa gaatggctgc catatttgta
      961 tctggtggtc caagagctgc aattggtcag taatgttatg tttcatacga atacaaaaac
     1021 tcgctatttt tagcgagttt tttttatctt tatcctcaaa attattcaaa gatgattaca
     1081 tcaaaaatta ttgcaaacgg tattttaaga gctattgcca ttttagtttt tacagcacta
     1141 tcattctatt ttttatattt aatccaatcg gttttaattt atctggtcgt ggctttaatc
     1201 cttactttaa taggaaatcc aatccttgat ttctttaaga aaaaattaaa attcaaacat
     1261 actttggcta ctattgcaac tgtttcaata tttattatgc ttattgctgg ttttattttg
     1321 atgtttatcc cattaattac ttctcaagga gaaaatttat ccttgctaaa atctgctgaa
     1381 atagaacgta atatcactca attaaaaacc caaactttca cctttttaga aaatcataat
     1441 attgacacct ccagaatgat gaaagaagcc aatttagctt ccaaaataaa tttaaatttt
     1501 atacctaatt tcttgaactc cattttagga accattagca gtttcggcat cggattagcc
     1561 tctgtattat ttattacttt tttctttctc aaagaccgat taatgttcat cattggtgct
     1621 aaaaaaataa ttccagatac tcacgaagag aagattctga attcagcaca caaaataaat
     1681 catttgcttt ctcgctattt tattggctta ttgcttcaat tatttattgt gtttattttg
     1741 tatatcattg tattgttcat ctttgatatt ccaaatttgt tcatcattgc gtttttatgt
     1801 gccgttttaa acattgttcc ctacattggc ccattaattg cgtcgatttt ggcagctatt
     1861 ctaacgatgt tgagcaactt aggttccgat tttcaaaccg taatattacc caccacaatt
     1921 tatgttttga taggattttg gattgtgcaa attattgaca acaacttgtc acaaccccta
     1981 attttttcta aaagtgttag ttcacatcct ttagaaattt tccttgttat cttgattgca
     2041 ggattcttat ctggaatttt aggaatgatc attgccgttc ctttatacac cattttgaaa
     2101 gttattggaa aagaattttt tccagaaaac aaggtgattc aattgtttac taaaaatatt
     2161 taattttgga tttaaaaata ctaaatcctg aaattcaaca atttatcaat aacaatcttg
     2221 ataaaaatat tgcccaatta gctttgcaaa aaaatccatt tcctgaaata gaatggatta
     2281 ctattttgaa tcaaattgaa gctaaaacca aatcaaaaga caaactacct cattggttta
     2341 cggctaaaaa catcatttat ccgagcaaaa tatcggttga acaaacctca tctgaaaaaa
     2401 cagcttccta caaatcagaa ctagtttctg gtgaaagtct aattgattta accggaggtt
     2461 ttggtgttga cgatttttat tttgccaaaa aaataaaaga ggtaactcat tgcgaaatca
     2521 atccagaact ctctcaaatc gttcaacaca acttcgaaca attaaacgta aaaaacattg
     2581 attgtcagtc gggagatagt ttagaaattt tggcaaaatc aaactctaaa tacgattgga
     2641 tttacattga tccttctaga agaaatgata ccaaaggcaa agtatttatg ctcaaagatt
     2701 gtttgcccaa tgttcctgaa catcttgatt ttttatttaa ttcttcggat aaaattctaa
     2761 tcaaaacagc acctctactc gatatttccg ctgggttatc tgaacttaaa aatgtaaaat
     2821 cgatacatat tgtagcttta gaaaatgaag tcaaggaatt attatgggaa ttacacagag
     2881 attattttgg aaaaacaaac ttaaaaaccg tcaatattgt caagaaaaat cgtgaaactt
     2941 tcgaatttgt tttggatgaa aattctataa ttcctgaata tggtttaccc gaaaaatatc
     3001 tttatgaacc caacagcgcc ataatgaaat ctggtggatt tgatgaagtg agtacatttt
     3061 ataaaattaa aaaactgcac aaacattcgc atttatatac caattcagaa attattcttt
     3121 ttcctggtcg aattttcgaa attgaaaata ctattcccta tcacaaaaat gacatgaaaa
     3181 tttttcttgc gggaaaacaa gctaatatca ccacccgaaa ttttccagaa acggttgaaa
     3241 gcattcgcaa aaaatggaaa ataaaagaag gtggacatca ctattgtttt tttacaactg
     3301 atgaaaatga caataaaata gtcttaattt gcaaaaaaat tacatcctaa tgaaacgagt
     3361 ttccctacta ttttttttac tactaagtag ttatttattt gcccaaaaaa cctgtgagta
     3421 tagcgccgac gtaaaagatt caattggaac ctataaattg accaaggaat atctaattta
     3481 cgaaaagaat ttcgctggga ataacagtta tatctttctc tctttgtctt tgaccgatgg
     3541 tactccaact ttgaatatac aaatgattca aaaaagcaaa gaatttatga aagccaattg
     3601 ttttgacaaa aattcaaagt tattttttca attaaataat ggaaaaatag taaccttact
     3661 acatattgat caagaaaatt gtggctcctt ggttcgggat ggaaatggct ttgacaacag
     3721 acttttagtc gggaatttta tgtttataaa aggtactatg gaagatttga aaagttctcc
     3781 tgtgaatcta atgcgtatca aatatttgac cgatatagaa gattatgtgt tacgcaaaga
     3841 aattacttcg gagcttgaca ctaaggtcta tgaacccgaa aattacttcc taaattattt
     3901 acactgcatt caataattaa tcacatttcc atttggcatt cgaaactttg tcgtttgaag
     3961 ccgttttcaa attgtaatgc caccactctg aatctaaagg attaaaacca ttttgaatca
     4021 ttgtggtttt taataattct ctgttatttt ttatttcctg cgaaagattt tgataattat
     4081 gactggcttc aataccaaaa aaatcaaaag aggttcccat atcgagtgct tttccgttag
     4141 catcgactaa agtgatatcg acagctcctc ctctattgtg aatagaacct ttggccggat
     4201 ca
//
LOCUS       DAOSNN010000228         4197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_21226_length_4197_cov_6.261226, whole genome shotgun sequence.
ACCESSION   DAOSNN010000228 DAOSNN010000000
VERSION     DAOSNN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21226_length_4197_cov_6.261226"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..672
                     /locus_tag="PLQ70_10375"
     CDS             187..672
                     /locus_tag="PLQ70_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HQE35062.1"
                     /translation="MAIHKLDFDEFDEIDYHLVAIHTSLEDYRLAYFINQKLPINLSI
                     FKDEIHINIKEGETKFSRFYYFDKENDISWNLIQNKNKVIQQKNETSQNLFSDVNMEV
                     ATKVYLLPEFKKVDYFLKIENIEEGLLTQMQLLLNTIENISTAYNVDTNKIKSKNNLI
                     F"
     gene            678..2108
                     /gene="pyk"
                     /locus_tag="PLQ70_10380"
     CDS             678..2108
                     /gene="pyk"
                     /locus_tag="PLQ70_10380"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQE35063.1"
                     /translation="MSTNKKTKIVATLGPACSTREIIKDMIDAGVNVFRINFSHADYN
                     DVKERIAIVRQLNEEFGYTTAILGDLQGPKLRVGVMEDGVIVNDGDLITFTTAEDIIG
                     TAQKVFMKYQNFPNDVNPGERILLDDGKLIFEIIETDKKSEVVAKVIQGGELKSKKGV
                     NLPNTKISLPALTEKDIADAIFAIEQKVDWIALSFVKTPRDLQDLHELIAKHSDEKIP
                     IVAKIEMPEALVNIDKIVAYCDALMVARGDLGVELPAHEVPLVQKELILRAKTARIPV
                     IVATQMMETMINSLTPTRAEVNDVANSVMDGADAVMLSGETATGLYPVQVIQKMTQIL
                     EAVEDSPLINVPQNTPQVRTNRFITKTICHHAALTANIIEAKAICTLTNSGYTAFQIS
                     AWRPSAHILVFTSNKRILTQLNLLWGVKSYFYDKMKSTDDTIADVNEIAREKGYVAKG
                     DFLINLAAMPLKDKGMVNTLRVSEVM"
     gene            2257..3030
                     /locus_tag="PLQ70_10385"
     CDS             2257..3030
                     /locus_tag="PLQ70_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HQE35064.1"
                     /translation="MAFESKNPFLNNKSFTATSASTDHKVHQATLIDYNQDMTLAGTI
                     NKTLILFLLLTAASLITWWMAFNGMNPMLPTIGGAIVGLILVVIAAFKPQYSPVLAPG
                     YALFEGLFIGGVSAIFEAKYPGIVIQAVGATFVTFAVCLGLYKFKIVKVTEQFKSVVV
                     AATLAIATYYLISWLFSMFTSFVPIHYGNSMMSIGISVFVIVIASLNLFLDFDRMEKG
                     VEQKMPKFMEWFAAMGLIITLVWLYIEFLRLLSKLSSRN"
     gene            complement(3082..4137)
                     /gene="dinB"
                     /locus_tag="PLQ70_10390"
     CDS             complement(3082..4137)
                     /gene="dinB"
                     /locus_tag="PLQ70_10390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQE35065.1"
                     /translation="MIHIDMDAFFASVEQMDNPDLRGKPIAVGGAENRGVVAAASYEA
                     RKFGVRSALSGVLAKKYCPDLIFVRPRFERYKEISNKIRKIFYEYTDLVEPLSLDEAY
                     LDVTINKKGNPSATLLAQEIRLRILNEVGLTASAGISVNKFVAKIASDYNKPNGQKTV
                     NPDEVIPFLEVLPIRKFYGVGKVTTEKMYQLGVFTGLELKSKSLEFLEKHFGKSGSFY
                     YNVVRGIHNSEVKSDRITKSVAAEHTFDINLSSEIFMIEQLENIANSLEKRLKKHHVA
                     GKTVTLKIKYSDFTQQTRSKTLPYFISDKGLILETVKELLYQERMKDSVRLLGISLSN
                     LNTEEKKTVVVQLKFAF"
BASE COUNT         1429 a          709 c          695 g         1364 t
ORIGIN      
        1 taaaaagaaa gcagaagaaa aagcatcaca acgagcctat tttgcctttc aaaagaaaat
       61 tgatttaaaa tagtcgaaat ttctttaatt tataacgttt tcgcaattaa aagagaaaaa
      121 acatagattt tgtcaggtta atgctccgtt agaaatgcta tctttacaac ttatttttta
      181 tagaaaatgg cgattcacaa attagatttt gacgaatttg atgaaattga ttatcaccta
      241 gttgctattc atacttcatt agaagactat cggttagcct attttattaa tcaaaagttg
      301 cctataaatt taagtatatt taaagatgaa attcatataa atataaaaga aggagaaaca
      361 aaattctcaa gattttatta tttcgacaag gaaaatgata tttcttggaa tttgattcaa
      421 aataaaaaca aggtaattca acaaaaaaat gaaactagtc aaaatctatt ttcagatgta
      481 aatatggaag tggcaacaaa agtgtatttg cttccagaat ttaaaaaagt ggattatttt
      541 ttaaaaatag aaaacataga agaaggtctt ttaacccaaa tgcaactttt attaaataca
      601 attgaaaaca tctcgacagc ttataacgtc gatacaaata aaataaaatc aaagaataat
      661 ttaatttttt aatatcaatg tcaacaaaca aaaaaacaaa aattgtagcc acacttggac
      721 ctgcatgtag tacaagagaa atcatcaagg atatgattga cgcaggagta aatgtgttta
      781 gaatcaattt ttcgcatgct gattacaatg atgtaaagga gagaattgct attgttagac
      841 aacttaacga agagtttggc tataccacag caattctagg agatttacaa gggccaaaac
      901 ttagagttgg cgttatggaa gatggggtaa tcgtaaacga tggcgattta ataactttta
      961 caactgccga agacatcata ggaactgcac aaaaagtttt tatgaaatac caaaattttc
     1021 caaacgatgt aaatcctgga gaacgtattt tacttgatga tggtaagctt atttttgaaa
     1081 ttattgaaac cgacaaaaaa tcggaagtag tagcaaaagt tattcaaggt ggtgaattaa
     1141 aatctaaaaa aggagtaaac cttccgaata caaaaatatc acttcctgct cttaccgaaa
     1201 aagacattgc cgatgccatt ttcgctatag aacaaaaagt tgattggatt gcactttctt
     1261 ttgtaaaaac tccaagagac cttcaagacc tacacgaatt aattgcaaaa cattctgacg
     1321 aaaaaattcc tattgttgcc aaaatagaaa tgccagaagc acttgtcaat atcgacaaaa
     1381 tcgtagctta ttgtgatgct ttgatggttg ctcgtggcga tttaggagta gaacttcctg
     1441 ctcacgaagt accattagta cagaaagaat tgattcttag agcaaaaact gctcgaattc
     1501 ctgtaattgt agccactcaa atgatggaaa caatgatcaa cagcttgaca ccaactcgtg
     1561 ccgaagtgaa tgacgtagcc aactcagtta tggacggagc cgatgctgta atgctttctg
     1621 gagaaactgc tacaggactt tacccagttc aagtaattca aaaaatgact caaattcttg
     1681 aagctgtcga agattcacca cttatcaatg tgccacaaaa cacaccacaa gttagaacaa
     1741 accgctttat tacaaaaacc atttgtcatc acgcagcgct tacagccaac atcatcgaag
     1801 caaaagccat ttgtacttta acaaacagtg gttatactgc tttccaaatt tcggcttgga
     1861 gaccatctgc acatattctt gtttttacat ctaataaaag aattcttact caacttaact
     1921 tattgtgggg agtaaaatct tatttttatg ataaaatgaa aagtaccgat gatactattg
     1981 ctgatgtaaa tgaaattgca agagaaaaag gatatgttgc caaaggagat tttcttatca
     2041 atctggctgc tatgccgcta aaagacaaag gaatggtaaa caccttgaga gtttcagaag
     2101 taatgtaatc taaattaatc gaatactaat taatccgtgt caattacaaa gtggcacgga
     2161 tttttttgtt tatacagcta atgaaaaaat ttaagaataa ataaacattc ttctaccgaa
     2221 aatattctat ttttgaacaa acaaacattt tatactatgg catttgaatc aaaaaatccg
     2281 tttttaaata ataaatcatt tacagccaca tcggcttcaa ctgatcacaa agtacatcag
     2341 gcaactctta ttgattacaa ccaagatatg actttggctg gcacaattaa taaaacatta
     2401 attctgtttt tattactcac agcagcctct ttgattactt ggtggatggc ttttaatggc
     2461 atgaatccaa tgttgcccac tattggcggt gctatcgtgg ggttaattct agttgtaatt
     2521 gctgctttca aacctcaata ttcgccagtt ttagctcccg gttacgcctt attcgaagga
     2581 ttattcatcg gaggagtttc agcaattttt gaagccaaat atcctggaat agtaatccaa
     2641 gctgttggag caacatttgt aacttttgca gtttgtttgg gattgtataa atttaaaatc
     2701 gtgaaagtta ctgagcaatt caaatcggtt gttgtagctg cgactcttgc tattgctact
     2761 tattatctaa tttcttggtt gttttcaatg tttacgagct tcgttcctat tcattatggc
     2821 aattcaatga tgagtattgg catcagtgtt tttgtgattg ttatagcctc tttaaatttg
     2881 tttttagatt ttgaccgaat ggaaaaaggt gtagaacaaa aaatgccaaa atttatggaa
     2941 tggtttgccg caatgggatt aataatcacc ttggtttggt tgtatatcga atttttaaga
     3001 ttgctttcta aactttcgag tagaaattaa attcagtttt taagatactt ataaccacgc
     3061 tttcgggcgt ggttttttgg tttaaaaagc aaattttaac tgcaccacaa ccgtcttttt
     3121 ttcttcggta ttgagattgc tcaaggatat tcccagtaat cgaacagagt ctttcattcg
     3181 ttcttgatac aaaagttctt tcaccgtttc tagaatcaaa cccttatcag agataaaata
     3241 aggcaatgtt ttgctgcgag tttgttgtgt aaaatcgctg tattttattt ttaaggtaac
     3301 tgtttttcca gcgacatggt gtttttttaa tcttttttct aatgaattgg caatattttc
     3361 taattgttct atcataaaaa tttcggaaga aagatttata tcaaaagtat gttcggctgc
     3421 caccgattta gtaatcctat ctgattttac ttcactatta tgaatgcctc tcactacatt
     3481 ataatagaaa cttcctgatt ttccgaaatg cttttctaga aactccaatg atttgctttt
     3541 aagttccaat cctgtgaaaa cacccaattg atacattttt tcggtggtga ctttcccaac
     3601 tccataaaat tttcgaatgg gtaaaacttc caaaaaagga ataacctcat caggattgac
     3661 tgttttttgt ccattaggtt tgttgtaatc gctagcaatt ttggccacaa acttattgac
     3721 agaaattcct gccgaagctg tcaaacccac ttcattgaga atgcgcaatc ttatttcttg
     3781 tgccaataag gtcgcacttg gattcccttt tttgtttatt gtaacatcga gataagcttc
     3841 gtcaagcgaa agaggctcaa ccaaatcggt atattcgtag aaaattttac gaattttatt
     3901 ggatatttct ttgtatcgtt caaatcgagg tcggacaaaa atcaaatcag gacaatattt
     3961 tttggccaaa accccactta gtgcgcttcg aactccaaat tttctggctt cataactagc
     4021 tgccgcaact acacctcgat tttctgctcc gccaacggct ataggctttc ctcgcaaatc
     4081 aggattatcc atttgctcta ccgaagcaaa aaaagcatcc atatcgatgt gtatgatttc
     4141 ctatttgaaa acgaatcttg catgttacaa atttaatcaa aaagtaataa tcgattt
//
LOCUS       DAOSNN010000229         4195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_21246_length_4195_cov_6.107005, whole genome shotgun sequence.
ACCESSION   DAOSNN010000229 DAOSNN010000000
VERSION     DAOSNN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21246_length_4195_cov_6.107005"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLQ70_10395"
     CDS             <1..725
                     /locus_tag="PLQ70_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HQE35066.1"
                     /translation="GTPLENITQITYQVPNFNTKINLLKHLVQTNEDMSRVLVFVNNK
                     KISDMVHTRIEEDFEGQFGVIHSNKSQNYRLSTMAEFQEGNLRGLITTDIMARGLDIS
                     NITHVINFEMPELPELYMHRIGRTGRADATGTAISFIAPREEEAKFAIELLMDMELPF
                     EDFPETVEISTKLIEPEKDRQPMKFMMKKVKLEGDGAFHEKSKKNKKVNLGGPGVTKK
                     KTHGSVNRNMLKNQAAKKKKNK"
     gene            complement(794..1906)
                     /locus_tag="PLQ70_10400"
     CDS             complement(794..1906)
                     /locus_tag="PLQ70_10400"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HQE35067.1"
                     /translation="MKNIYAAILSFLTFATMQSQETKLNLIVGTYTKTCESKGIYVYE
                     FDSKTGDFSLKNNTENILNPSYLSVSNDNKFVYSVSENDKKSSVSAFGFNSKSGKLDF
                     INYQNPNGLNPCYIINDDKNVITANYSSGSISVLGKKNDGSIGEVKQVVQHVGKSING
                     SRQEAPHAHMVCFSPDKKYVLANDLGTDKVYLYQYNPNVANEVLVLKNAVPVKPGSGP
                     RHLILSKDGKFVYLLQEMDGTLTSFSYADGTLKKVSETTVVASDFKGDIGAADIHISP
                     DGKFLYATNRGTANDISVFKILKNGKLEFVQRTSTLGKGPRNFTIDPTGNFLLVGHQY
                     TNDIVIFKRDSKTGEITDTGKKIALCSPVCLVFTKI"
     gene            2047..3975
                     /locus_tag="PLQ70_10405"
     CDS             2047..3975
                     /locus_tag="PLQ70_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HQE35068.1"
                     /translation="MQFKHPEILYFLFLLVIPILVHLFQLRRFKKEYFTNVRFLKELS
                     IQTRKSSKIKKWLLLATRLLLLTCIIIAFAQPFFKAKNSINSANEMYIVLDNSFSMQA
                     KGQKGELLKRAVQELLENTPENQTFSLITNSETFWNTDIKSIRTELQNLKYSALPFQL
                     ESAMAKIKSRKSAFNKDIIVITDAVGLEAKQLKSINSDFNTYFIIPEAEQKNNTAIDS
                     VFIHQNLDNFYEIGLNLSAFGEKNKDIPVALYNQNKLIAKTQTKFETNKKTIYFTIPK
                     KDFHGYASITDNGLEYDNNFYFSISKPKKNNIISIGTAEKSNFLSRIYTNDEFNFNNF
                     DIASLDYNAVEKQDAIVLNELDEIPQALQTTLKSFVEKSGNLIFIPSAKTSTANINPF
                     LKIFGNLQFKSLENKEKQITKINFNHPLFSGVFENKINNFQYPKTKTSFGIASTNPAV
                     LYYEDQTAFLTSINNPISAVYLFTAPINLENSNFQQSPLIVPTFYKMGISNGNNGVNA
                     LSISDNNPFYVDANLSKEEILKIKNDTETFIPVQQILNGKVKMMFNDLPQQAGNFGVF
                     NKAENIKNISFNYDRAESDLDKVNESALSDYKTIDSVNTVFNSIQTDRTDNQIWKWFV
                     IFALLFLLTEMAIIRFVK"
BASE COUNT         1472 a          753 c          653 g         1317 t
ORIGIN      
        1 caggaacacc tttggaaaac attacacaaa ttacctatca agttcctaac ttcaatacca
       61 aaatcaattt attgaaacat ttagtacaaa cgaatgaaga tatgagtcgt gtcttggttt
      121 ttgtgaacaa caagaagatc tcggatatgg ttcatactcg aattgaagaa gattttgaag
      181 ggcaatttgg agtgattcac tctaataaat ctcagaatta tcgtttgagc acgatggcag
      241 agtttcaaga aggcaacctt cgcggattaa tcactaccga cattatggcg agaggtttgg
      301 atatttcgaa tattacgcac gttatcaact tcgaaatgcc agaacttcct gaattgtata
      361 tgcaccgtat tggtcgtact ggtcgtgccg atgctacagg aacagccatc agttttatcg
      421 ctccacgtga agaagaagcc aaatttgcta ttgaactttt gatggatatg gaattacctt
      481 tcgaggattt ccctgaaact gtagaaattt caacaaaact aatcgaacct gaaaaagacc
      541 gccagccgat gaagtttatg atgaaaaaag taaaattaga aggcgatggc gcttttcacg
      601 aaaaaagcaa aaagaataag aaagtcaact tgggtggtcc tggtgtaacc aaaaagaaaa
      661 cacacggttc tgtgaacaga aatatgttga aaaatcaggc cgctaaaaag aaaaaaaata
      721 aataaatttt accgcaaatt cgcaaattaa acccagctaa gaatgtatta aatttgcgaa
      781 tttgcggtta aaattaaatt ttggtgaaaa ccaaacaaac tggcgaacac aatgctatct
      841 ttttacctgt atctgtaatc tctccagttt ttgaatctct tttaaaaatt acaatgtcat
      901 tggtatattg atgtcccact aaaaggaaat ttcctgttgg atcaatcgta aaatttcgag
      961 gcccttttcc caaagtgctg gttctttgca cgaattctaa ttttccattt ttcaaaatct
     1021 tgaaaaccga aatatcattg gcagttcctc gattggtagc gtacaaaaat ttcccgtcag
     1081 gcgaaatatg aatgtcggca gcaccaatat ctcctttaaa gtctgatgca acaacggtgg
     1141 tttcactcac ttttttcaat gttccgtcag cataactgaa tgaagttaac gttccatcca
     1201 tttcttgcaa caaatagaca aacttgccat ctttgctcaa tatcaaatgt cttggtccac
     1261 ttccgggttt tacaggaaca gcatttttta aaaccaaaac ttcgttcgcg acattgggat
     1321 tatattgata caaatacact ttatcggttc ctaaatcatt ggccaaaaca tactttttat
     1381 ctggcgaaaa acacaccata tgggcgtgag gtgcttcttg tcgactgcca ttaatacttt
     1441 ttccaacgtg ttgcaccact tgcttcactt cgccaatact gccgtcattt tttttaccca
     1501 agaccgaaat actaccgctt gaataattgg ccgtaatcac gtttttatca tcgttaatga
     1561 tgtaacaagg attcaatccg tttggatttt gataattaat aaaatctaat tttccacttt
     1621 ttgaattaaa tccaaaagcg ctaaccgaac tttttttatc gttttcactt accgaataga
     1681 cgaatttatt gtcattggaa accgataaat aactgggatt taaaatgttt tcggtgttgt
     1741 ttttaagact gaaatcccca gttttagaat cgaattcgta aacgtaaatt cctttgcttt
     1801 cacaagtttt agtataagtc ccaacaatta aattgagttt ggtttcttgt gattgcattg
     1861 ttgcaaaagt taaaaaacta agaatagcag cataaatatt tttcataatt atcaaatcta
     1921 ttgggctaag ataaaacatt tttttaccac aaatttgcaa attattccaa ttaaaattat
     1981 tttaactctg cgaatctgcg gtctaatttt tatttaaaaa taaaatcaaa atactatctt
     2041 tgaaaaatgc aattcaaaca cccggaaatc ctttattttc tctttttatt ggtaatacca
     2101 attctggttc atttgtttca attgcgacgt tttaaaaagg aatattttac caatgttcgt
     2161 tttctaaagg aactttccat tcaaacccga aagagttcca aaattaagaa atggttgctt
     2221 ttagcaacac gtttattgtt gttgacttgt attattattg cttttgcgca accttttttc
     2281 aaagccaaaa acagtataaa cagtgccaac gaaatgtata tcgttctgga taattccttt
     2341 tcgatgcaag ccaaaggtca aaaaggagaa ttgctaaaaa gagccgttca ggaattacta
     2401 gaaaacacgc ccgaaaacca gactttttcc ttaattacca attccgaaac gttttggaat
     2461 accgacatca aatccattcg aaccgaatta caaaacctga aatacagcgc cttgcctttt
     2521 caattggaaa gtgcaatggc gaaaataaaa tcccgaaaat cggctttcaa caaagatatt
     2581 attgtgatta ccgatgctgt tggtctagaa gcgaaacaat tgaaaagcat caacagcgat
     2641 ttcaatacct atttcattat tcccgaagcg gaacaaaaaa ataatacggc tattgacagt
     2701 gtttttattc atcaaaattt ggataatttt tatgaaattg gcttgaattt atccgctttt
     2761 ggagaaaaga acaaagacat tcctgtggct ttatacaatc aaaacaagct gattgcaaaa
     2821 acgcaaacga aatttgaaac caataaaaaa actatttatt tcacgattcc aaaaaaggat
     2881 ttccacggtt atgcttctat taccgacaat ggattagaat acgataacaa cttttatttc
     2941 agtatttcaa agcctaaaaa gaacaatatc atcagcattg gaactgctga aaaatcaaac
     3001 tttttatcga gaatttatac taatgacgaa ttcaatttta ataattttga catcgcttct
     3061 ttagattata atgccgtcga aaaacaagac gctatcgtat tgaatgaatt ggatgaaata
     3121 ccgcaggccc tacaaaccac tttgaaatca tttgtagaaa aaagtggaaa tttgattttt
     3181 attccatcgg cgaaaacttc cacagcgaat atcaatccgt ttttgaaaat ctttggaaac
     3241 ctccaattca aatctttaga aaacaaagaa aaacagatta ccaaaatcaa cttcaatcat
     3301 cctttgttta gcggtgtttt cgagaataaa atcaataatt ttcaatatcc aaaaaccaaa
     3361 acttcctttg gaatagcaag tacaaatcca gctgttttgt attatgaaga ccaaacggct
     3421 tttttaactt caattaataa tccgatttct gcagtttatc tttttacggc tccaatcaat
     3481 ttggaaaatt ctaattttca acaatcgcct ttaattgttc ctacctttta caaaatggga
     3541 atcagcaatg gaaacaatgg cgtgaatgca cttagtatta gcgataacaa tcccttttat
     3601 gtagatgcca atttatccaa agaagaaatt ctgaaaatca aaaacgatac agaaactttt
     3661 attcctgtac aacaaatttt gaacggaaaa gtaaaaatga tgttcaatga tttgccacaa
     3721 caagcgggga attttggagt tttcaacaag gcagaaaaca taaagaatat tagtttcaac
     3781 tacgacagag ccgaaagtga tttagataaa gtgaacgaaa gtgctctttc tgactataaa
     3841 actattgatt cggtaaatac cgttttcaat agcatacaaa ccgaccgaac agacaatcaa
     3901 atttggaaat ggtttgttat atttgcattg ctttttctgc tgacggaaat ggcaattata
     3961 cgattcgtta agtaaaattc atatataatt ttaccgcaaa ttcgcaaatt attctaattg
     4021 taagaaatta aaaatctgcg gtctaaaaaa tatacaaata attcaataat tattatgaac
     4081 acaatcctcc gagaagcgaa aataattgat tctgaaagtc cttttcacaa tcagactgtc
     4141 gatattttaa ttgttgatgg catcatccaa aaaattggaa cttcacttcc taatt
//
LOCUS       DAOSNN010000230         4195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_21249_length_4195_cov_4.392512, whole genome shotgun sequence.
ACCESSION   DAOSNN010000230 DAOSNN010000000
VERSION     DAOSNN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21249_length_4195_cov_4.392512"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..694)
                     /locus_tag="PLQ70_10410"
     CDS             complement(158..694)
                     /locus_tag="PLQ70_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQE35069.1"
                     /translation="MRKLENSELDRKSIEDFKQSEKTPIIIVLDDIRSLHNIGSVFRT
                     ADAFLIEKIYLCGITATPPNKEIHKTALGATETVAWEHHKDVLEVIQNLKNEKVSVLA
                     IEQVESAVFLQDFEIEKDKKYALVFGNEVFGVAQEAVELCDGCIEIPQLGTKHSLNIS
                     VSAGIVVWDLFKKMKWNL"
     gene            complement(769..1239)
                     /locus_tag="PLQ70_10415"
     CDS             complement(769..1239)
                     /locus_tag="PLQ70_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQE35070.1"
                     /translation="MKKLITLSIALLAFTFSNAQETSKATKKTAKKTATTKVTTPALP
                     KVEGAGMVFANETIDYGTIAHNADGKREFVFTNNGNKPLIITNAVGSCGCTVPTFPKE
                     PILPGAKASIGVKYATDRIGAFTKTVTITSNAEGTPSKTLTIKGTVLGDDAPKS"
     gene            1391..3997
                     /gene="mutS"
                     /locus_tag="PLQ70_10420"
     CDS             1391..3997
                     /gene="mutS"
                     /locus_tag="PLQ70_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044047604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQE35071.1"
                     /translation="MSSKEKVVKETPLMKQYNEIKRKYPDACLLFRVGDFYETFGEDA
                     IRASKILGITLTKRGAGSETETALAGFPHHSINTYLPKLVKAGLRVAICDQLEDPKMT
                     KTIVKRGVTELVTPGVSMNDEVLQSKSNNFLASVYFSNKLIGISFLDVSTGEFLTAQG
                     NAEYIDKLLQNFSPSEVLVPKNNKNDFRETFGDDYHSFYLEDWIYKEDYAFETLTKHF
                     QVVSLKGFGVEELKEGIIASGAILYYLSETQHNKVQHITSIQRIAEDAYVWMDRFTIR
                     NLELYHSYNPNAVTLLDVIDKTLSPMGGRLLKRWLALPLKDANKISGRHQVVAYLKEN
                     QEVLKNIQKQIKQISDLERLISKIAAGKVSPREVVYLKESLDAIIPIKTLALESPQEA
                     VKVIGDSLHSCDLLREKIKTVLNQDAPVAITKGNAIAKGVNEELDDLRAISTSGKEFL
                     EGIEKRESERTGISSLKISFNNVFGYYIEVRNTHKDKVPAEWIRKQTLVSAERYITEE
                     LKEYETKILGAEEKIYKIEVELFEQLVSWIALYIKPVQMNANLVAQLDCLCSFTQLAI
                     ENKYVCPELDETFELNIKNGRHPVIEKQLPVGVPYIANDVFLDRETQQLIMITGPNMS
                     GKSAILRQTALIVLLAQMGSFVPADSVRMGIIDKIFTRVGASDNISMGESTFMVEMNE
                     TASILNNISDRSLVLLDEIGRGTSTYDGISIAWAIAEFLHEHPSQPKTLFATHYHELN
                     EMSESLTRIQNYNVSVKELKDTVLFIRKLVKGGSAHSFGIHVAKMAGMPQIVILKAQK
                     LLKKLEKDHSNEALNGIKSSKDEMQMSFFNLDDPLLEEIKDEILSLDINTITPMEALM
                     KLNEIKRMLTKK"
BASE COUNT         1358 a          700 c          841 g         1296 t
ORIGIN      
        1 aaattcgcat ctggaattgt agtatattgg gcttgcccaa aaaaactgga caaaaggata
       61 aacagtaaaa aaagtttttt catagcttag gttttcttct aaagaaaagg ttgcctaaga
      121 tataaaaaaa cttcaaatta atttgataaa aatataatta caaattccat ttcatttttt
      181 tgaataaatc ccaaacgact attccagcac ttaccgaaat gttcaaagaa tgtttggtgc
      241 ctaattgcgg aatttcgata cagccatcac agagttcaac ggcctcttgt gctactccaa
      301 agacttcgtt tccaaaaact aaggcgtatt ttttatcttt ttcaatttca aaatcttgaa
      361 gaaaaacggc gctttctact tgctcaatgg caagtacact aactttttcg ttttttaaat
      421 tttgaatcac ttccaaaacg tctttgtgat gttcccaagc cacagtttct gtagcgccaa
      481 gagccgtttt gtgaatttct ttgttaggcg gcgtagccgt gatgccacac aaatatattt
      541 tttcaatcaa aaaagcatcg gctgttctaa aaaccgaccc aatgttgtgt aaacttcgaa
      601 tgtcatccaa aacaatgata attggtgttt tttcggattg tttaaaatct tcaatggatt
      661 ttcgatcgag ttcactgttt tcaagctttc tcataaggtt ttgaagtgta tttaaataaa
      721 aaaagcttcc aaaaagaggg aagctttttt ataagattat tttaaaattt agctttttgg
      781 agcgtcgtct cctaaaacag ttccttttat agtaagtgtt tttgatggag ttccttcagc
      841 atttgaagtg atagttaccg tttttgtaaa agcaccaatt ctatctgtag catatttcac
      901 tccgatagaa gcttttgcgc caggaagaat aggttctttt gggaaagttg gaacagtaca
      961 tccacaagaa ccaacagcat tagtgataat caaaggcttg ttaccgttgt tagtaaaaac
     1021 aaattcacgt tttccatcag cattgtgagc aattgtaccg taatcaatgg tctcgtttgc
     1081 aaataccatt cctgcacctt ctacttttgg taaagcagga gttgttactt tggttgtagc
     1141 tgttttttta gctgtttttt ttgtggcttt tgaagtttct tgagcattag agaatgtgaa
     1201 tgccaatagt gctattgaaa gtgtaattaa ttttttcatc attttttagt ttagaatgag
     1261 tcacaaattt atataaaaaa acaaaacgcc atcctaaaat tttcttaaaa tgatttttaa
     1321 attctaggct gtatattatt cgccataaaa tctataaatt cgctttctgt aaaatttaaa
     1381 aaatagcatt ttgtcatcaa aagaaaaagt ggtcaaagaa acacctttaa tgaagcaata
     1441 caacgaaatc aaaaggaaat atcctgatgc ttgcttgttg tttagggtgg gggattttta
     1501 tgaaacattt ggcgaagacg ctattcgtgc ttccaaaatt ctgggaataa ccttgaccaa
     1561 acgtggtgca ggttccgaaa ccgaaacggc tttggcaggt tttccacatc attccatcaa
     1621 tacctatttg ccaaaattgg taaaagcagg acttcgagtg gcaatttgcg accagctcga
     1681 agatccaaaa atgaccaaaa ccatcgtgaa acgtggtgtt acggaattgg tgactccagg
     1741 cgtttcaatg aacgacgagg tgttgcaatc caaatcgaat aattttctgg catctgttta
     1801 tttttccaat aaattgattg gaatttcatt tttggatgtt tccacgggcg aatttcttac
     1861 cgcccaaggc aacgccgaat atatagataa attgttgcag aatttcagtc cgagcgaggt
     1921 gctggttccc aaaaacaaca aaaatgattt ccgtgaaact tttggggatg attaccacag
     1981 tttttatctc gaagactgga tttacaaaga agattacgct ttcgagactt taaccaagca
     2041 ttttcaggtt gtttccctga aaggttttgg ggtcgaagaa ttaaaagaag gaattattgc
     2101 ttctggagcg attttgtatt atttgtccga aacgcagcat aacaaagtac agcacatcac
     2161 gtcaattcag cgtatagccg aagatgctta tgtttggatg gatcgattca cgattcgaaa
     2221 tctcgaattg tatcatagct ataatccaaa tgcagtgaca cttctcgatg ttattgataa
     2281 aacgctttcg ccaatgggtg gtcgtttgct caaacgttgg ttggctttgc ctttgaaaga
     2341 tgccaataaa attagtggtc gtcatcaagt agttgcttat ttgaaagaga atcaagaggt
     2401 cttgaaaaac attcaaaagc aaatcaaaca aatctccgat ttggaacgtt tgatttcgaa
     2461 aattgcggca ggaaaagttt ctccgcgtga agtcgtttat ttgaaagaat ccctagatgc
     2521 cattattccg ataaaaactt tggctttaga aagtccacaa gaagcggtaa aagttattgg
     2581 agacagcttg catagttgtg atttattacg ggagaaaatc aaaaccgtct tgaatcagga
     2641 tgcgccagtg gctattacca aaggaaatgc cattgctaaa ggtgtaaatg aagaattaga
     2701 tgatttaaga gcgatttcca cttcgggaaa agaattttta gaaggaattg aaaaaagaga
     2761 atccgaaaga acgggtattt catccttgaa aatctccttt aacaatgttt ttggctatta
     2821 cattgaagtt cgaaacacgc ataaagacaa agtaccagca gaatggattc ggaaacaaac
     2881 attggtcagt gctgagcgtt atattactga ggaattaaaa gaatacgaaa ccaaaatcct
     2941 cggtgccgaa gaaaaaatat ataaaataga agtcgagcta ttcgaacaat tggtaagctg
     3001 gattgcactt tacatcaaac cagttcaaat gaatgccaat ttggtggcgc aattggattg
     3061 tttgtgttct tttacccaat tggctatcga aaataaatac gtttgtcctg aactcgacga
     3121 aacattcgaa ttgaatatca aaaacggaag gcatcctgtg atcgaaaaac aattgccagt
     3181 gggtgtgccg tatattgcca atgatgtttt cttggataga gagactcaac agttgataat
     3241 gattaccgga cccaatatgt ctggaaagtc ggctattttg cgtcaaaccg ccttgattgt
     3301 gctcttggct caaatgggaa gttttgttcc ggcagacagc gtgagaatgg ggattatcga
     3361 caaaatattt accagagtgg gagcttcgga caatatttcg atgggagaat ctacgtttat
     3421 ggtcgaaatg aatgaaacgg cttctatctt gaacaatatt tctgatagaa gtttggtttt
     3481 gctagatgaa atcggaagag ggacgagtac ctatgacgga atttccatag cctgggcgat
     3541 tgccgaattt ttgcacgaac atccatcgca acccaaaaca ctttttgcga cgcattatca
     3601 cgagttgaac gaaatgagtg aatcgttgac tcgaattcag aattacaatg tttcggtcaa
     3661 agaattaaaa gatacagtgc tttttattcg aaagctggta aaaggaggaa gtgctcacag
     3721 ttttggtatt cacgtggcaa aaatggcggg aatgccacaa atcgtgattt tgaaagcgca
     3781 aaagcttttg aagaaattag aaaaagacca ttccaatgaa gctttgaacg gaatcaaatc
     3841 gtctaaggat gaaatgcaaa tgagtttctt taatctggac gatccattat tagaagaaat
     3901 caaagacgag atattgagtt tagacatcaa caccattaca ccaatggaag ctttgatgaa
     3961 actcaatgaa atcaaaagaa tgttgaccaa gaaatagttt ctttgtgttt aaagttaggg
     4021 ttatcagaag gttatatttt gtctgtatat tttttgcaaa aaaggcttgt ttaattgaat
     4081 taatgtatta aatttgcatc cgcaatacgg aacaagtatt gagttctttt tagaatgtaa
     4141 atgcgaaaat agctcagttg gtagagcgcg accttgccaa ggtcgaggtc gcggg
//
LOCUS       DAOSNN010000231         4160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_21465_length_4160_cov_4.697199, whole genome shotgun sequence.
ACCESSION   DAOSNN010000231 DAOSNN010000000
VERSION     DAOSNN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21465_length_4160_cov_4.697199"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..570)
                     /locus_tag="PLQ70_10425"
     CDS             complement(121..570)
                     /locus_tag="PLQ70_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQE35072.1"
                     /translation="MSLSIQIMEEIKTAMRAKDTVALEALRAIKSEILLAQTSSGSKE
                     EITEADEIKLLQKLVKTRKDSAKIFIEQNRMDLAEPELAQIAVIEKFLPAQLSEAEVE
                     AVIAKIIAETGASGIASMGKVMGIASAQLGGTAEGKTISTIVKKLLV"
     gene            complement(762..2723)
                     /gene="ftsZ"
                     /locus_tag="PLQ70_10430"
     CDS             complement(762..2723)
                     /gene="ftsZ"
                     /locus_tag="PLQ70_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQE35073.1"
                     /translation="MMSNSEFGSISFDLPKNQSNVIKVIGVGGGGSNAINHMFKQGIK
                     GVDFIVCNTDSQALDNSAVPNKIQLGVNLTEGLGAGANPDVGQQSAIESISDIEKMLD
                     SNTKMVFITAGMGGGTGTGAAPVIAQLAKERDILTVGIVTLPFSFEGKVRLEQAHIGI
                     KKLRKQVDSLIVINNNKLREVYGNLGFKAGFSKADEVLATASRGIAEVITHHYTQNID
                     LKDAKTVLANSGTAIMGSAVATGANRAKEGIISALDSPLLNDNKITGAKNVLLLIVSG
                     SDEITIDEIGEINDYIQAEAGHNANIIMGVGEDESLGESISVTIIATGFDIEQQHEIV
                     NVEPTKIIHTLDDEQKSNAFDLTKNTVTSFNNPIDSPIAKTEERIVFELLEEDEIVEE
                     IVEEPAKVVLKSEELMGMNEFIKNLDVTFEIVSPIKDLDFVVSSPEVKQIEVVEPKFF
                     HKEEQASFSFDLPLFKSEPVKSEPVQLEETKTLFELSKETRDIKVNEAVQFVPVTELT
                     DNGIIRHTLEEYTEIENTFMNSKPTPAVVEEVIPEELNITMKKIDVIENTTSTLESIS
                     PMDMTIQESMKLRTEERRKKLKEFNYKFHNNVSRIDELEREPAYKRHGIDLSKSQLSS
                     TNSRISVGTDSNDDLQLRSNNSFLHDNVD"
     gene            complement(2764..4146)
                     /gene="ftsA"
                     /locus_tag="PLQ70_10435"
     CDS             complement(2764..4146)
                     /gene="ftsA"
                     /locus_tag="PLQ70_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQE35074.1"
                     /translation="MEKENIAVGLDIGTTKIVAMIGKKNEYGKLEILGVGKSKSLGVA
                     RGVVNNITQTIQSIQQAVLEAENKSGYKINDVVVGIAGQHIRSIQHSDYIIRTNAEEV
                     ISNYDIQLLIDQVNKLAMLPGEEIIHVLPQEYKIDGQADIKEPVGMYGGRLESSFHVV
                     VGQASSIRNVARCIQSSDIELSGLTLEPLASANAVLSQEEKEAGVALIDIGGGTTDLA
                     IFKDGIIRHTAVIPFGGNVITDDIKEGCSIIENQAELLKVKFGSAWPGENKDIEIVSI
                     PGIRGREPKEISLKNLSKIIHARVNEIIEQVFSEIKDYGHDDPRKKLIAGIVLTGGGA
                     QLKHIKQLVEYITGMDTRIGYPNEHLAGNSDEEISCPLYATAVGLVMNSIENKTQSAI
                     KIDKNAGPKIVTPPVFQPQEEPIVTEKIEDLNIPIATKKTTEETSNKKNFFDLSGYLG
                     KIKEFLDNAE"
BASE COUNT         1107 a          768 c          680 g         1605 t
ORIGIN      
        1 cccaaccctc agagccgaaa tctgatattc tatccagttg aactacgcag ccattttata
       61 aatctcaaat tttaaaattt caaattccaa tattggtgtt ggaatttgga ttttaaacta
      121 ttaaaccaat aattttttaa cgatggtaga aatggttttt ccttcggctg tgcctcctaa
      181 ttgtgctgag gctattccca ttactttccc catagaggct attcctgatg cgccagtttc
      241 agctataatc tttgcaatta cggcttctac ttcggcttcg gatagttgtg ctggcaagaa
      301 tttttcgatt acagcaattt gagccaattc tggttctgcc aaatccatac gattttgctc
      361 gataaaaatc ttggcactgt ccttacgggt tttaaccaat ttttgaagta attttatttc
      421 gtctgcttcg gtgatttctt cttttgatcc agatgaagtt tgagctaaaa gaatttctga
      481 tttaatggct cttaaagctt ctaatgcaac ggtatctttg gctctcatgg cggtttttat
      541 ttcctccatg atttgtatag ataagctcat aataatttat gatttagatt tatgatttta
      601 gaatgtttaa accccaaata ttgggttgca ttcgggagca caaatttata gtaatttaat
      661 cgaattagca actgattata gtagcgttcc ttgttctgat accaaaaaat aacccgaaaa
      721 ttgaataaat tttcgggtta gacaactttg gtttgaatta gttagtcaac attatcgtgc
      781 aaaaatgaat tatttgatct taattgtaga tcatcattgc tgtctgttcc aactgaaatc
      841 ctagaatttg tactagatag ttgtgatttt gaaagatcta ttccgtgtct tttgtaggca
      901 ggttctcttt ctagttcgtc aattcttgaa acgttattgt gaaatttata gttgaattct
      961 tttaattttt ttcttctttc ttcagttctt aatttcatcg attcttgaat ggtcatgtcc
     1021 ataggagaaa tactttctag tgttgaagtg gtgttttcaa tcacatcaat ttttttcatt
     1081 gtgatgttca attcttctgg aattacttcc tcaacaactg caggagttgg tttagagttc
     1141 ataaaagtat tttctatttc agtatattct tcaagagtat gtctaataat tccattgtcg
     1201 gtcaattctg ttactggaac aaattgcaca gcctcattga ctttgatgtc gcgcgtttct
     1261 ttacttaatt caaaaagcgt tttggtttct tctaattgta caggttctga ttttacaggt
     1321 tctgatttaa aaagaggtaa atcaaatgaa aatgaagctt gttcttcttt gtggaaaaat
     1381 tttggctcaa caacctctat ttgttttact tctggtgacg aaacaacaaa atcaagatct
     1441 tttataggag aaactatttc gaatgtaaca tctaagtttt tgatgaattc attcataccc
     1501 ataagttctt cacttttgag tacaactttt gcaggttctt caacgatttc ttcaacgatt
     1561 tcatcttctt ctaataattc aaaaacgatt ctttcttctg tctttgcaat tggtgaatcg
     1621 ataggattat tgaaagatgt aactgtgttt tttgtcaaat caaacgcatt acttttttgt
     1681 tcgtcatcaa gagtgtgaat gatttttgtt ggctcaacat ttacaatttc gtgttgttgt
     1741 tcgatatcaa atccggtggc aataatggta actgaaatag attctccaag cgattcatct
     1801 tcaccaactc ccataataat attggcatta tgtcccgctt cggcttgaat ataatcgttg
     1861 atttctccaa tttcatcaat tgtaatttca tctgaaccag aaacgatgag caacaatacg
     1921 tttttagctc cagtaatttt attgtcgttc aataaaggag aatccaaggc agaaattatt
     1981 ccttctttag ctctgttggc tccagtagcg acagccgatc ccataatagc agttccgctg
     2041 ttggctaata ctgttttggc atcttttaaa tcgatatttt gcgtgtagtg atgtgtaatt
     2101 acttctgcaa ttcctcttga ggcagttgct aatacttcgt cagcttttga gaatccagct
     2161 ttaaaaccta gattaccata cacttctctc aatttgttat tgttgataac aattagtgaa
     2221 tcgacttgtt tgcgtaattt ttttattcca atatgagctt gttctaaacg aactttccct
     2281 tcaaatgaga atggtaaagt tacgattcca actgttaaga tgtctctttc tttggccaat
     2341 tgagcaatta ctggcgctgc acctgtaccg gttcctccac ccattccagc ggtgataaaa
     2401 accattttgg tgttactatc aagcattttt tcaatatcag aaatgctttc tatagcagat
     2461 tgttgtccta catctggatt tgcacctgcg ccaagtccct cggttaggtt tactcctaat
     2521 tgaattttgt tgggcacagc actgttgtcc aaagcttgtg aatcagtatt acaaacgata
     2581 aaatctacgc ctttaatacc ttgtttgaac atgtggttta tagcgttgct tccgccgccg
     2641 ccaacaccta ttacttttat tacatttgat tggttttttg gtaaatcaaa tgaaatgctg
     2701 ccaaattctg agttgctcat catcttatgg attttattat ttttaattta ttttttttat
     2761 tctttattct gcgttgtcta agaattcttt aatttttcct aagtaaccac ttaaatcaaa
     2821 aaagtttttt ttgtttgaag tttcttcggt tgttttttta gtagcaattg ggatatttaa
     2881 gtcctcaatt ttttctgtaa caattggctc ttcttgaggt tgaaatactg gaggtgtaac
     2941 gattttaggt ccagcgtttt tgtctatttt gatagcactt tgagttttgt tttcgatgct
     3001 attcattact aaacctactg ctgtggcata caatgggcat gaaatttctt cgtcagagtt
     3061 gccagctaaa tgttcgtttg gataaccaat tcgtgtatcc attccggtga tgtattctac
     3121 taattgtttg atgtgtttta attgggcacc tccaccagtt aatacaattc cagctattag
     3181 ttttttgcga ggatcgtcat gtccataatc ttttatttct gaaaaaactt gctcaataat
     3241 ttcattcaca cgcgcatgaa ttattttcga taaattttta agagaaattt ctttaggttc
     3301 tcttcctctt attcctggaa tagaaacaat ttcaatgtct ttattttctc caggccaagc
     3361 tgatccaaat ttcactttca ataattcggc ttggttttct ataatcgagc agccttcttt
     3421 gatgtcatcg gtgattacat taccaccaaa agggattact gctgtgtggc gaataatacc
     3481 gtctttgaat atggctaaat cggttgttcc tccgccaata tcaatcaatg caactcctgc
     3541 ctctttttct tcttggctta aaacagcatt tgcagatgct aaaggttcta aggttagacc
     3601 tgacaattcg atatctgaac tctgaatgca tcttgcaaca tttcgaattg aagatgcttg
     3661 ccctacaaca acgtgaaaac tagattctaa tcttccaccg tacattccta ccggttcttt
     3721 gatgtctgct tgtccgtcaa ttttatattc ttgtggcaaa acatgaataa tttcttctcc
     3781 aggtaacatt gccaatttgt ttacttggtc tatcagaagt tgaatgtcat aattgctaat
     3841 tacttcttca gcatttgttc tgataatata atcactgtgt tgaatgcttc gaatgtgttg
     3901 accagcaata cctactacaa catcgtttat tttgtaacct gatttgtttt ctgcttcaag
     3961 aacggcttgc tgaatggatt gaatggtttg agtaatgttg tttacaactc ctcttgccac
     4021 acctaaactt ttggattttc caacccctaa aatttctaat tttccgtact cgtttttctt
     4081 gccaatcatg gcaactattt ttgtcgtccc aatatctaga cctactgcaa tattctcttt
     4141 ttccattatc tattatttag
//
LOCUS       DAOSNN010000232         4150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_21555_length_4150_cov_6.228816, whole genome shotgun sequence.
ACCESSION   DAOSNN010000232 DAOSNN010000000
VERSION     DAOSNN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21555_length_4150_cov_6.228816"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..89)
                     /locus_tag="PLQ70_10440"
     CDS             complement(<1..89)
                     /locus_tag="PLQ70_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQE35075.1"
                     /translation="MISEKLKKGKLLIAEPSIIGDLSFNRSVI"
     gene            complement(206..478)
                     /locus_tag="PLQ70_10445"
     CDS             complement(206..478)
                     /locus_tag="PLQ70_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQE35076.1"
                     /translation="MNKSELIDAIAADAGITKAAAKLALESFLGNVGSTLKKGGRVSL
                     VGFGSWSVSSRAARDGRNPQTGKTIKIAAKNVVKFKAGAELEGAVN"
     gene            complement(661..1608)
                     /gene="fmt"
                     /locus_tag="PLQ70_10450"
     CDS             complement(661..1608)
                     /gene="fmt"
                     /locus_tag="PLQ70_10450"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQE35077.1"
                     /translation="MEKLRIIFMGTPEFAVGILDTLIKANYNVVGVITVADKPAGRGQ
                     KIKYSDVKEYALANNLPLLQPTNLKDETFLAELKAWNANLQIVVAFRMLPEVVWKMPK
                     LGTFNLHASLLPNYRGAAPINWAIINGDAKTGVTTFFIDDKIDTGAMILSAETEIDPN
                     ENAGLLHDRLMRLGSETVIETLNLIENGNVNATIQEENAEIKTAYKLNKENCKIDWTQ
                     SAREIFNLIRGLSPYPSAWCIFQDKNEEWNVKIHEAKLVLENHNFESGSLICSKKEMK
                     IAVKDGFIQVISLQFPGKKKMNVGELLNGMTFSDNAKVV"
     gene            complement(1608..3503)
                     /locus_tag="PLQ70_10455"
     CDS             complement(1608..3503)
                     /locus_tag="PLQ70_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HQE35078.1"
                     /translation="MPTALQILQKYWKHDKFRSPQDEIIHSVLDGKDTFGLMPTGGGK
                     SICFQVPALMKDGICLVISPLVALMKDQVQRLQSMNIKAIALTGGIQSDEMITLLDNC
                     EFGNYKFLYLSPERLQSDWILERIKNLPINLIAIDEAHCVSQWGHDFRPAYLKISKLK
                     THFPKVPFLALTASATQRVLEDVILQLGLENPVVFKKSFARKNIAYMVFEVEDKLYRM
                     EQILKKNPQPSIIYVRNRKSCSDTASQLQALGFKATFYHGGLSVKEKEKNMSLWMNEE
                     AQIMVSTNAFGMGIDKSNVKTVIHIHLPESLESYYQEAGRAGRNDEKAFAIILTSPSD
                     KMQAQSQFIDVLPDKKILTQIYIKLCNYFRIAYGEGIDEQFSFNLNHFCQKYGFPTLK
                     TYNAIQFLDRQGILTLSQEYSEKITMQFIIESKEVIRYMSLNPNEEPVILSLLRNYPG
                     IYESQTAVNTSLIAKKSNCEEALVHSILDKLKERDLIDYHKKNNDASIIFNEVREDER
                     TINRVSKYLENQNKQKVAHFESVVHYINEKKVCKSKLILDYFGEKTETDCGICSYCIS
                     RKKKSDDELLVPKKIMQLLKIQELDSREIQKLTKNSEDDVIFALQTLLENNKIIIQAN
                     NKYTLKS"
     gene            complement(3567..4106)
                     /locus_tag="PLQ70_10460"
     CDS             complement(3567..4106)
                     /locus_tag="PLQ70_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQE35079.1"
                     /translation="MSKEIIVIIGGPGTGKSTIIDGLVEKGFCCYPEISRQVTLEAKK
                     QGIEQLFLENPLLFSELLLEGRKNQFKSALEETHDTVFIDRGIPDVLAYMHYIGDSYP
                     AHFDLACRENLYTKIFILPPWEEIYVSDEARYENFEQAKLIHNHLVETYQNYGYELIE
                     VPKDTLDNRILFILDEISR"
BASE COUNT         1237 a          755 c          675 g         1483 t
ORIGIN      
        1 aaaattactg acctattgaa agacaaatct ccaattatag aaggctctgc aataagtagt
       61 tttccttttt ttaatttttc tgaaatcata ggttgtattt taattaaatt taagaaaaaa
      121 agattaaaat aaaacaaata aatcgataaa aagtataaaa aaaccttcca taggaaggtt
      181 ttatatattt taagaaagcc ttaaattaat ttacagctcc ttctaactca gctccagctt
      241 taaatttcac tacatttttt gcagcaattt ttatagtttt tcctgtttga gggtttctac
      301 catctcttgc agctcttgaa gaaactgacc atgatccgaa acctactaaa gatactcttc
      361 cgcctttttt caaagtacta cctacgtttc ctaaaaatga ttctaaagct agtttagcag
      421 cagcttttgt aattcctgca tcagcagcga tagcgtcgat taattctgat ttgttcataa
      481 tgtttagtta ttaaatgttg gttaaaaaaa ctttgttaat aataacaaaa ttagtaggat
      541 taacttccct tacaagtgtt ttgtctaatt ttagcctaat ttgttgataa cttgtttttt
      601 ttgttcataa gccgatgtaa aatcatcata aaacacccta aaatccccgt ttttgttggt
      661 ctaaaccacc tttgcattat cagaaaaagt cattccattc aataattctc ccacattcat
      721 cttctttttt ccaggaaatt gaaggctaat cacctgaata aaaccatctt ttacggcaat
      781 tttcatttct tttttactgc aaatcagact tccagattca aaattgtgat tttctaatac
      841 caatttcgct tcgtgaatct taacattcca ttcttcattt ttgtcctgaa aaatgcacca
      901 agccgatggg tatggactta gtcctctaat taaattaaat atttctctgg ccgattgggt
      961 ccaatcgatc ttgcaatttt ctttgtttaa tttgtatgcc gtttttattt cagcattttc
     1021 ttcctgaatg gttgcgttaa catttccatt ttcgattagg ttcaatgttt ctataaccgt
     1081 ctcgcttccc aatcgcatca atctatcatg caaaagtcct gcgttttcgt ttggatcaat
     1141 ttcggtttcg gcgctaagaa tcatggctcc tgtatctatt ttatcatcaa taaaaaaagt
     1201 ggtaacccct gtttttgcat caccattaat tatcgcccaa ttaattggag cagcacctcg
     1261 ataattagga agtaatgagg catgaagatt gaatgtgcct aatttcggca ttttccaaac
     1321 gacttcgggc aacattctaa aagcaaccac gatttgtaaa ttggcattcc atgcttttaa
     1381 ttcggccaaa aaagtttcgt cttttaagtt ggtgggttgc aataaaggaa gattattagc
     1441 caaagcatat tcttttacat ccgaatattt tattttctgg ccgcgacctg ctggtttatc
     1501 ggcaacggta ataactccaa cgacattata attggctttg atgagggtat ctagaatgcc
     1561 aacggcaaat tctggagttc ccataaaaat gattcttaat ttttccatta tgattttaaa
     1621 gtgtatttgt tgttggcttg aatgattatt ttattgtttt ctaataaagt ttgcaaagca
     1681 aagataacat cgtcttcact atttttggtc agtttttgaa tttccctcga atccaattct
     1741 tggattttta atagttgcat tattttttta gggactaaca attcatcatc agacttcttt
     1801 tttcgggaaa tacaatacga acaaattcca caatcggttt ctgttttttc tccaaaatag
     1861 tccaaaatca atttgctttt gcaaactttc ttttcgttaa tataatgcac aacagattcg
     1921 aaatgagcta ctttttgttt gttttggttt tccaaatatt tcgaaacccg gttgatagtt
     1981 ctttcgtctt cacgcacttc attaaaaata atcgaagcat cattgttttt tttgtgataa
     2041 tcaataaggt cgcgttcttt taacttatct aaaatagaat gaactaaagc ctcttcgcaa
     2101 tttgattttt tggcaatcaa tgatgtatta accgcagttt gcgattcata aattccagga
     2161 tagtttcgaa gtagtgataa aataacaggt tcttcatttg ggtttaaact catatagcga
     2221 attacttctt tggactcaat gatgaattgc atcgtgattt tctctgaata ttcttgtgac
     2281 aaagtcaaaa ttccttgacg atccaaaaac tgaatagcat tgtaggtttt caatgtagga
     2341 aagccatatt tctggcaaaa gtgattcaga ttgaacgaaa actgttcgtc tatcccttcg
     2401 ccataagcaa ttcggaaata gttgcacagt ttaatgtaaa tttgggtcaa gatttttttg
     2461 tcgggcaaca catcaataaa ttgactttgg gcttgcattt tatcagatgg actcgttaga
     2521 ataatggcaa aggctttttc atcatttcta cccgctcgac ctgcttcctg ataataactt
     2581 tctaaacttt cgggtagatg aatatgaatg accgttttta cattcgattt gtcaattccc
     2641 attccaaagg cattggtcga aaccataatt tgtgcttctt cattcatcca aaggctcata
     2701 tttttttctt tctccttaac agataatccg ccgtggtaaa aagtggcttt gaatcctaaa
     2761 gcttgcaatt gtgaagcggt atccgagcac gattttctat ttcgtacata tattatagaa
     2821 ggctgcggat tctttttcaa gatttgctcc attcgataca atttgtcttc gacctcaaaa
     2881 accatataag caatgttttt tcgggcaaat gattttttga aaaccacagg gttttctaat
     2941 cccaattgaa gaatcacatc ctcaagaact ctttgggtcg ccgaagcagt tagagctaga
     3001 aaaggaactt tcgggaaatg agtttttagt tttgaaattt ttaaataagc aggacgaaaa
     3061 tcgtgtcccc attgcgatac acaatgcgct tcgtcgatgg caattaaatt aataggaaga
     3121 tttttgatgc gctctaaaat ccaatcggat tgcaggcgtt ctggtgataa gtataagaat
     3181 ttataattgc caaattcaca attatccaaa agtgtaatca tttcgtccga ttgaattccg
     3241 cctgtcaatg caatagcttt gatgttcata ctttgcagcc gttgcacctg atctttcatc
     3301 aaggcaacca agggagaaat gaccaagcaa attccgtctt tcatcaaggc aggaacctga
     3361 aaacaaattg attttccgcc accagtaggc atcagtccaa aagtatcttt accatccaaa
     3421 accgaatgga taatttcgtc ttgtggcgac ctaaatttgt cgtgtttcca atatttttga
     3481 agaatttgta atgcggttgg catagatttc agattttaga tttcagattt ttgtagaaat
     3541 cctaaattta aattctactt tgtaaattat ctggaaattt catctaagat aaataaaatt
     3601 ctgttatcga gggtgtcttt tggaacttct atgagttcat aaccataatt ttgataggtt
     3661 tcaacgaggt gattgtgaat gagtttcgct tgttcgaagt tttcataacg ggcttcgtcg
     3721 ctcacatata tttcttccca aggcggaagg ataaagattt ttgtgtataa attttctcgg
     3781 caagccaagt caaaatgtgc gggataactg tcgccaatat agtgcatata agccaaaaca
     3841 tctggaattc cacggtcaat aaaaacagta tcgtgggttt cttctaaagc acttttgaat
     3901 tgattttttc gaccttctaa taataattcg ctgaaaagca aagggttttc aaggaacaat
     3961 tgctcgatgc cttgtttttt tgcttctaag gtaacttgtc tggaaatttc gggataacag
     4021 caaaaacctt tttctactaa tccatcgata atggtgcttt tgcctgttcc tggtccgcca
     4081 atgatgacta taatttcttt actcacgttt ttcaaataaa gcgcaaattt acctaaactt
     4141 attgggattc
//
LOCUS       DAOSNN010000233         4101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_21889_length_4101_cov_4.402620, whole genome shotgun sequence.
ACCESSION   DAOSNN010000233 DAOSNN010000000
VERSION     DAOSNN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21889_length_4101_cov_4.402620"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1815)
                     /locus_tag="PLQ70_10465"
     CDS             complement(<1..1815)
                     /locus_tag="PLQ70_10465"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HQE35080.1"
                     /translation="MKLNDILKDSNYKLTQFKPEKITRFEDSIFLKEIKGLETPYIIC
                     LVRKKEIKLTPEEAVRQLYLMVLNEEYEYPFERMELEYAVTFGREKKRADIVIFDKQN
                     TTSVNIMVELKKPKLKDGKEQLKSYCNATGAPIGIWSNGDSISFYNRRDPNYFKDIPE
                     IPKASQKLTDVLTERWTIDDLIAKDKLVNERKSLKDLILEMEDEVLANAGVDVFEELF
                     QLIFTKLFDELESTRNKSRSLEFRNYGETETELKNKIQSLFEKAKAKWEGVFADSAKV
                     TLTPSHLAICVSSLESIKLFNSNLDVVDEAFEYLINKSSKGEKGQYFTPRYVIDMCVK
                     MLDPKAEETVIDTAAGSCGFPVHAIFHVWEKQLKEKGLTKSHLFTAEEKLPEQTDYVK
                     NNVFAIDFDEKAVRVSRTLNLIAGDGQTNVLHLNTLDFERWDDNYNDNNWLDVYGNGW
                     KNLRKQLKDTKAKTESIEEERNGKIYVKDTQRYNCRDFQFDVLMANPPFAGDIKETRI
                     ISKYELGKKPTGKYQNAVGRDILFIERNLDFLKPGGRMAVVLPQGRFNNSSDKQIRDF
                     IAEHSRILAVVGLHGNVFKPHTGTKTSVLFIQKWDDKLC"
     gene            1917..2171
                     /locus_tag="PLQ70_10470"
     CDS             1917..2171
                     /locus_tag="PLQ70_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQE35081.1"
                     /translation="MKRIRKTEIPQAVIELGNRIKDIIKEKNLVSKNVAYDAGLDVEN
                     LRKYIKGIQEMKITTMLKIASALDVEPSELIKGLKIDFGK"
     gene            2330..>4101
                     /locus_tag="PLQ70_10475"
     CDS             2330..>4101
                     /locus_tag="PLQ70_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HQE35082.1"
                     /translation="MKTQLFLITPPFTQLNTPYPATAYIKGFLNTKNISATQADLGIE
                     VILKLFSKEGLIDLFQSNSQQPTTDNCKRIFALQDEYIKTIDSVIAFLQGKNPTLSLQ
                     ICQEDYLPEASRFAQLEELDWAFGTMGTQDKAKHLATLYLEDISDFIVECVDANFGFS
                     RYAERLGRSANSFDELYSALQEEPTYIDEVLFSLLKDRIETIEPELFLISVPFPGNLY
                     SAFRSAQYVKKQYPNIKIAMGGGFPNTELRSLSDARVFEFFDYITLDDGELPVELLME
                     AVGKPESFEGQFKRTFLLENGKVVYKNNSTRNDYKQSEVGTPDYSDLCLDKYISVIEI
                     VNPMHRMWSDGRWNKLTMAHGCYWGKCTFCDISLDYIKVYEPIAANLLCNRMEEMIAQ
                     TGQNGFHYVDEAAPPALMRALALEILRRKLSVTWWTNIRFEKSFTADLCLLLKASGCI
                     AVSGGLEVASDRLLELIDKGVTVEQVAKVTRNFIEAGIMVHAYLMYGYPTQTVQETVD
                     SLEMVRQLFEAGVLQSGFWHQFAMTAHSPVGMYPEKFGVVPTSPPLEGLGEVFANNDI
                     NYVDKTGIDHEKFSFGLKKSLFNF"
BASE COUNT         1235 a          701 c          758 g         1407 t
ORIGIN      
        1 acacaattta tcatcccatt tttgtataaa taatacagaa gttttggttc ctgtgtgtgg
       61 tttgaaaaca tttccgtgca aaccaactac tgccaaaatt ctagaatgtt cagctataaa
      121 atctctaatt tgtttgtcgc tactgttgtt aaaacgccct tgtggcaaaa ccactgccat
      181 tcttccacct ggtttcaaaa aatcgaggtt tcgttctata aaaagaatgt ctctgcctac
      241 ggcgttttgg tatttacctg ttggtttttt gcctaattca tatttagata taatgcgtgt
      301 ttctttaatg tctccagcaa atggagggtt tgccatcaat acatcaaact gaaaatctct
      361 gcaattgtaa cgttgagtat cttttacata aatttttccg ttgcgttctt cttcgatact
      421 ttctgtttta gctttggtgt ctttgagttg tttacgtagg tttttccatc catttccata
      481 aacatcgagc caattgttat cattgtaatt gtcgtcccaa cgttcaaaat ctaaagtatt
      541 tagatgtaat acattggttt gtccgtcgcc tgcaataagg tttaaggttc tagagacacg
      601 tacagctttt tcgtcaaaat cgatggcaaa aacattattt tttacataat cggtttgttc
      661 gggtagtttt tcttcggcag taaaaagatg gcttttggtt aatccttttt ctttgagttg
      721 tttttcccaa acgtgaaaaa tggcgtgaac aggaaagcca caacttcccg ctgctgtatc
      781 aattacggtt tcttcggctt ttgggtcaag catttttaca cacatatcaa ttacataacg
      841 aggtgtaaaa tattgccctt tttcgccttt gctacttttg tttatcaaat attcaaaggc
      901 ttcatccact acatctaaat tggaattaaa caatttgata ctttctaaag atgaaacaca
      961 aatggctaaa tgcgatggtg taagtgtaac tttggcacta tcggcaaaaa cgccttccca
     1021 tttggctttt gctttttcaa ataatgattg tattttgttt tttagttcgg tttcggtttc
     1081 gccatagttt ctaaattcta acgaacgaga tttatttcgg gtactttcta attcatcaaa
     1141 taattttgta aaaatcagtt gaaataattc ttcaaataca tcaacaccag cattagctaa
     1201 tacttcgtct tccatttcaa gaattaaatc tttcaaagat ttacgttcgt tgactagttt
     1261 atctttagca attaaatcat caattgtcca gcgttcagtc aaaacatcgg ttaatttttg
     1321 cgatgctttt ggtatttcag gaatgtcttt aaaatagttt gggtcacgtc tattgtaaaa
     1381 cgaaatactg tcgccattac tccaaattcc tattggagca cccgttgcat tgcaatagga
     1441 tttcaattgt tcttttccgt ctttcaattt tggttttttt agttcaacca ttatattgac
     1501 agaagtggtg ttttgtttgt cgaaaataac aatatcggca cgtttttttt cacgcccaaa
     1561 agttactgcg tattctaatt ccattctttc aaatggatat tcatattctt cattcaatac
     1621 cattaagtac aattgacgaa cggcttcttc tggtgttaat ttgatttctt tttttctaac
     1681 caaacagatt atatatggtg tttctaatcc tttaatttct tttagaaaaa tactgtcttc
     1741 aaatcttgtt attttttcag gtttgaattg agtgagtttg taattggaat cctttagaat
     1801 atcgttaagt ttcattttta tgtctgattt tcttcaaatg taaaaataaa aacggttgta
     1861 aacaatcgat tgaatacaat cggttaaaaa caatcttgac atcataaatt tatcttatga
     1921 aaaggataag aaaaacagaa attccacaag ctgttattga actgggtaat aggataaaag
     1981 atattattaa agagaaaaat cttgtatcta aaaacgtggc atatgatgca ggattagatg
     2041 ttgaaaattt gagaaaatat atcaaaggaa ttcaagaaat gaagattaca acgatgttga
     2101 aaattgcatc agctcttgat gtagaaccat ccgaactaat caagggttta aagattgatt
     2161 ttggaaaata gcaaatgtta tcatttagta aaacaaagtt ttctttggta cgattacagc
     2221 atatataaga cttgttctgt gtgttttttt acaaaatttc acttaccaat tgttttgaaa
     2281 aaacagtaaa caccaaattt tagttttact tttgcacaaa acaatttctt tgaaaaccca
     2341 actcttcctc atcacgccac cctttaccca actgaataca ccgtatccag caactgctta
     2401 tatcaaaggg tttctgaata ccaaaaacat ttcggcgaca caagcagatt taggtattga
     2461 agtgattttg aaattgtttt cgaaagaagg attaatcgat ttatttcaat cgaatagcca
     2521 acaaccgaca actgacaatt gcaaaagaat tttcgctctc caagacgaat acatcaaaac
     2581 cattgattcg gttattgcat ttttgcaggg aaaaaatcca acattgtcac tccaaatttg
     2641 tcaggaagat tatttgccag aagcttcgcg ttttgcacaa ttagaagaat tggattgggc
     2701 ttttggtacg atgggaacac aggacaaagc caaacatttg gcgactctat acctcgaaga
     2761 tatttccgat tttattgtgg aatgtgtcga tgccaatttt ggattcagcc gttatgcgga
     2821 gcgtttgggc agaagtgcta attctttcga cgaattatat tcggcattac aggaagaacc
     2881 aacttatatt gacgaagtat tattctcgct tttaaaagat aggatagaaa ccatcgaacc
     2941 cgaattgttc ttgatttctg tcccttttcc ggggaattta tattcggctt ttcgttcggc
     3001 gcaatatgta aaaaaacagt atccaaatat taaaattgca atgggtggcg gttttccgaa
     3061 taccgaattg cgttcgcttt ctgatgcccg agtttttgag tttttcgatt acatcacttt
     3121 ggacgatggt gaattaccgg tagaattatt aatggaagca gttggtaaac ccgaatcttt
     3181 tgaaggacaa ttcaagagaa ctttccttct cgaaaacgga aaagtggttt acaaaaacaa
     3241 ttccactcga aatgactaca aacaatccga agttggaacg cccgattatt cggatttgtg
     3301 tttggataaa tacatttcgg ttattgaaat agtgaatcca atgcaccgaa tgtggagcga
     3361 cggacgttgg aataaattga cgatggcgca cggatgctat tggggaaaat gtacgttttg
     3421 tgatatttca ttagattata tcaaagttta cgaacccatc gccgccaatt tattgtgcaa
     3481 tagaatggag gaaatgatag cgcaaacagg tcaaaatggt tttcattatg tagatgaagc
     3541 agcaccacca gctttgatgc gcgctttggc tttagaaatt ttacgcagaa aattatcggt
     3601 tacttggtgg actaatattc ggtttgagaa aagttttacg gcagatttgt gtttattgct
     3661 aaaagcttct ggttgcattg ctgtttctgg aggactcgaa gtggcctcgg acagattgtt
     3721 agaactaatt gacaaaggcg taacggtgga gcaagtggca aaagtcaccc gaaattttat
     3781 cgaagccgga attatggttc acgcttattt gatgtacggt tatccaacgc aaacggttca
     3841 ggagacggtg gacagtctgg aaatggttcg ccaattgttt gaagcaggcg ttttacaatc
     3901 aggattttgg catcaatttg cgatgacagc tcatagtcca gttggaatgt atccagagaa
     3961 atttggtgtg gttcctactt cccctccttt ggaggggtta ggggaggttt tcgccaataa
     4021 cgatattaat tatgtggaca aaacgggaat cgatcacgag aaattcagtt ttggattgaa
     4081 gaaatcgctt ttcaatttta t
//
LOCUS       DAOSNN010000234         4072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_22103_length_4072_cov_5.202141, whole genome shotgun sequence.
ACCESSION   DAOSNN010000234 DAOSNN010000000
VERSION     DAOSNN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22103_length_4072_cov_5.202141"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLQ70_10480"
     CDS             <1..660
                     /locus_tag="PLQ70_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQE35083.1"
                     /translation="GSDRIGIVAYAGSAFPVLPITSDYGVAKMFLQGMNTDIVSSKGT
                     SLDEAIKLSATYFDEKSKTSKLLIMISDGEDHSEGAEDAAEEAAKLGMKIITIGVGTE
                     KGGAIPLRVNGVVESFKRDNNNEVVITKLNKEGLTTIAKATKGGYVDGNNTKQVLDYV
                     KNALDNIQKTEFESTQMANFQSQFQWFLAFAFVLLFLDVFLLERKTKWVQKLNLFNEK
                     E"
     gene            660..1433
                     /locus_tag="PLQ70_10485"
     CDS             660..1433
                     /locus_tag="PLQ70_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE35084.1"
                     /translation="MKNLILYILVLLSFAVSAQEKDKTLPKANDEYAQKQFVEAEANY
                     RISHSKFPKRMVAPFNLGNAIYKQNQTSEAKYAYAKALENTKSRVQRHKVFHNLGNVF
                     MKEKNYSAAVEAYKDALRNKPSDEETRYNYALAKKMLKENPPKNDKNKDKNKDKNKDK
                     NKKDKDKDKKDDKKDGDKDKKDNKDGKDKDKKDDKGDKDKNGEKPKDDGKPKPMPGGI
                     SKQRTDNLLDAVNNEEKKIQDKMNAQKVKGKPVQTEKDW"
     gene            1493..3250
                     /locus_tag="PLQ70_10490"
     CDS             1493..3250
                     /locus_tag="PLQ70_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014085335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HQE35085.1"
                     /translation="MKRYLIIVLLSFQSLLAQVQFEAKVSKTTLGLNERLRIDFVMNM
                     DGDNFNQPSFEGFRVIAGPSQQVSQSWVNGRSSFEKIYSYYLLPMQKGTLIIKQATIE
                     YNGQIYKTSPVKINVTNAVQEARDPNDPTPISADNNLYLVAEVSKANPYVNEPITVVY
                     KLYVSYDIGVSNWRELDKPKYNDFWSQNIDVKQLVVEQGMFKGEKYQYMVLRKTVLYP
                     QKSGKLVIEPLSLDIDVQLPTNRRDMFGRVLLTNGNKRVSTGTKTINVKALPEAGKPA
                     DFSGAVGKFDFRVIPSKTNLKSGESLDLLVNVTGSGNMKLFNLPKPVVPNSLEMYDPV
                     HDEKINTTLSGMSGKIADKYTIIPQFKGKYPIKPLQFSYFDLGSGTYKTITSPEIMIN
                     VLDGPTSNDVVASTPTGISKNSISSSEQFKYIKSKTDLVSMKETDFLGSKTFYGLLLL
                     PFLILPLIVLLKKKKEALDSDVFGNKIKMNNKLAKKYLSEAKKQINNKEPFYVALEKA
                     MHNFLKAKLHIETSDMSKSNIQELLASKNVNAQTIIDFITLTENCELARYALTSSSVI
                     HQDYDKAVMIISDLEKQIT"
     gene            3371..>4072
                     /locus_tag="PLQ70_10495"
     CDS             3371..>4072
                     /locus_tag="PLQ70_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE35086.1"
                     /translation="MRKILFILLLTTQFFFAQSGFEKGNELYQKGKYEQAITEYETVL
                     ASHKHSAELYFNLGNCYYKLNKVASAIYNYEKALVLNPDDAEVQNNLKFAQKLQIDEI
                     KEVPQVGFSKMLHDFTSIFHYNTWAWIGVGLSFLFLLFFIGYYFSQTALFKRIFFIGM
                     FVFLFLVVVSVSSAISGKNHYENEKPAIVFATATEVKSEPQKAGNTAFILHEGTKVFV
                     LEALDNWKKIQLTDGT"
BASE COUNT         1549 a          646 c          758 g         1119 t
ORIGIN      
        1 ggtagtgaca gaataggaat tgttgcctat gctggaagtg cctttccggt tttgcccatc
       61 acttcagatt atggtgtggc aaaaatgttt cttcaaggaa tgaataccga tattgtttct
      121 tcaaaaggaa cctcattgga tgaagccata aaattgtcgg ctacttattt cgacgaaaaa
      181 agcaaaacca gtaagttgtt aatcatgatc tccgatggag aagatcattc agaaggtgcc
      241 gaagatgcag ctgaagaagc agccaaactg ggaatgaaaa tcattaccat cggtgtggga
      301 accgaaaaag ggggggcaat tccattgaga gtcaacggtg ttgtcgaaag ttttaaaaga
      361 gacaataaca acgaagtggt gattacaaaa ctcaacaaag aaggtttgac aactattgcc
      421 aaagccacaa aaggcggtta tgttgacgga aataatacaa aacaagtatt ggattatgtg
      481 aaaaatgcac tcgataatat tcaaaaaaca gaattcgaat cgacacaaat ggctaatttt
      541 caatcccaat ttcagtggtt tttggctttt gcttttgtct tgttgttttt agatgttttc
      601 ttgttagaaa ggaaaacaaa atgggttcaa aaattgaatt tatttaacga aaaagaataa
      661 tgaagaattt aattttatat atactagtat tactttcttt tgcagtttcg gctcaagaaa
      721 aagataaaac tttgccaaaa gcaaacgatg aatatgctca aaagcagttt gtcgaggctg
      781 aagccaatta caggatttcg cattccaaat ttcctaaaag aatggttgct cctttcaatt
      841 taggaaatgc aatatacaaa caaaaccaaa cctctgaggc caaatatgct tatgcaaaag
      901 cattggaaaa cactaaatct agagttcaga gacataaagt atttcacaat ttgggaaatg
      961 tttttatgaa ggaaaaaaat tattcggcag ctgttgaagc ctataaagat gctttgcgaa
     1021 acaaaccgtc agacgaagaa acgcgctata attatgcttt ggccaaaaaa atgctaaaag
     1081 aaaacccgcc taaaaacgat aaaaataaag acaagaataa agataaaaat aaggacaaga
     1141 acaaaaagga taaagacaaa gacaaaaagg acgataaaaa agacggagat aaagataaaa
     1201 aggataacaa agacggtaaa gacaaagata aaaaggacga taaaggcgat aaagataaaa
     1261 atggcgaaaa gcctaaagat gacggtaaac caaaaccaat gcctggtgga atttctaaac
     1321 aaagaacgga taatcttttg gatgccgtaa acaatgaaga gaagaaaatt caggacaaaa
     1381 tgaatgctca aaaagtaaaa ggcaaaccag ttcagacaga gaaggattgg taagatttta
     1441 aaatggttta aagaatttta aacacttaaa cgattaaaca gttacaccag taatgaaaag
     1501 gtatttaatt atagtattat taagttttca atcgcttttg gctcaagtac aatttgaagc
     1561 aaaggtgagc aaaaccacgc tgggtcttaa cgaaagactt cgcatcgatt ttgtaatgaa
     1621 tatggatggt gacaacttca accaaccttc tttcgaagga tttagagtta ttgctggacc
     1681 cagtcagcaa gtgagtcagt cgtgggtaaa tgggagaagt tctttcgaaa aaatctattc
     1741 ctattatctt ttgccaatgc aaaaagggac tttaattatc aagcaggcta ccatcgaata
     1801 taatggtcag atatataaaa catcacccgt taaaattaat gttaccaatg cggttcaaga
     1861 agcaagagat ccaaacgatc caactccaat ttctgcagat aacaacctct atctagtagc
     1921 cgaagtttct aaggctaatc cgtatgtaaa tgagccgata acagtggttt acaaattgta
     1981 tgttagctat gatatagggg tatcgaattg gagagaattg gacaaaccca aatacaatga
     2041 tttctggagc caaaatattg atgtgaaaca acttgtggtt gaacaaggaa tgttcaaagg
     2101 cgaaaaatat caatatatgg tactccgaaa aaccgtttta tatcctcaaa aatcaggtaa
     2161 actggtcata gaacctttgt cattggacat cgatgtacaa ttgcctacaa atagaagaga
     2221 tatgtttggt agagttttgc ttaccaatgg caataaaaga gtttcaacag gaaccaaaac
     2281 aataaacgtc aaagcacttc cagaagctgg aaaaccagcc gatttttcgg gagctgtagg
     2341 gaaatttgat tttcgggtaa ttccatccaa aacgaatttg aaaagtggtg aaagcttgga
     2401 tttattagta aatgttacag gaagcggcaa tatgaaattg tttaacttgc caaaacctgt
     2461 agttcccaat tcattagaaa tgtatgaccc tgttcacgac gaaaaaataa ataccacttt
     2521 gtcaggaatg tcgggtaaaa tagcagacaa atacaccatt attccacaat tcaaaggtaa
     2581 atatcccata aaaccattgc aattttccta ttttgattta ggatcaggaa cctataaaac
     2641 cataacttca cccgagataa tgattaatgt tttggatggc ccaactagca atgatgtagt
     2701 ggcttcaacc ccaacaggaa tttcaaaaaa cagcatttca agtagtgaac aattcaagta
     2761 tattaagtca aaaaccgatt tagtttcaat gaaagaaacc gatttcttgg gttcaaaaac
     2821 tttttatggt ttgttgttat tgcccttttt aatccttcca ttgatcgtat tgctcaaaaa
     2881 gaaaaaagaa gcattagaca gcgatgtttt tggaaataaa atcaaaatga acaacaaatt
     2941 ggcgaagaaa tatttatcag aagccaaaaa acaaatcaac aacaaagaac cgttttatgt
     3001 ggctttggaa aaagcaatgc acaatttcct aaaagccaaa ttgcatattg aaacctcgga
     3061 tatgagcaaa agtaacattc aggaattgtt ggcttccaaa aatgtaaatg ctcaaacgat
     3121 tattgatttt attacactta ccgaaaattg tgaactggca cgttatgcat taacatcgag
     3181 ttcggttatt catcaagatt atgataaagc ggtaatgata atttcggatt tagaaaaaca
     3241 aattacttaa aatagtacac ggataaaacg gatgcggata gcacagataa aaacagatta
     3301 aaaaatccgt aataatctgt gtctttgatt gatcgaatct gcgtcatccg cgttccaaaa
     3361 aacatacata atgagaaaga tactttttat attactacta acaactcaat ttttctttgc
     3421 tcaaagtggc tttgaaaaag gaaatgaatt gtaccaaaaa gggaaatacg agcaagccat
     3481 tacggaatac gaaacggttt tggcgagcca taaacattcg gcagaattgt atttcaattt
     3541 aggaaattgt tattataaat taaacaaagt agcttcagct atttacaatt acgaaaaagc
     3601 attggtacta aatccagacg atgctgaagt acaaaacaac ctaaaatttg cacaaaaact
     3661 acaaatcgac gagataaaag aagtgcctca agttggtttt tcaaaaatgc tacacgactt
     3721 tacatccatc tttcattaca atacttgggc ttggattgga gttggattgt catttttgtt
     3781 tttgctattt tttataggct attatttttc tcaaacggcc ttgttcaaaa ggatattttt
     3841 catcggaatg tttgtctttc tttttttagt tgtggtaagc gtttcgtctg ccatttcagg
     3901 taaaaatcac tatgaaaatg aaaaaccagc aattgttttt gcaacagcaa cagaggtaaa
     3961 aagtgaacca caaaaagcag gtaacaccgc atttattttg cacgaaggca ctaaagtttt
     4021 tgttttggaa gctttagaca attggaaaaa aattcaattg accgatggaa cc
//
LOCUS       DAOSNN010000235         4045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_22312_length_4045_cov_3.747619, whole genome shotgun sequence.
ACCESSION   DAOSNN010000235 DAOSNN010000000
VERSION     DAOSNN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22312_length_4045_cov_3.747619"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="PLQ70_10500"
     CDS             complement(<1..1645)
                     /locus_tag="PLQ70_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HQE35087.1"
                     /translation="MKKLKPQFLFLFILIPFLQVSAQENLTKLNDGIEIKIKKPQQND
                     AAVLRIQVVSNAILHITASPTSTFDTTKSLMLVDLKPNVAKFDVKSTNESVTVSTETL
                     NATVSIETGEIIFKDATGKTLLQETKGGGKTITGTTLDGKQSYIVKQSFNGETDEALY
                     GLGQHQNGAFNYKGTKVLLSQYNTEVAIPFLVSSKNYGILWDNNSLTKSIDTQNYDKI
                     STLRLYSAAGDKGWLTATYTDKKDPEKVLTRPESYIDYSYIPSLKQLPEGIKLENTLV
                     KWEGSFESDFSGEHQFNLWLSGYTNVYIDDKLVTARWRQGWNATNELIRINLEKGVKH
                     KLKIEWDPLGNESFISFTWSKPKTEAQKNQFAFESEAGQKIDYFFVSGKNIDEVISGY
                     RTLTGKATMMPKWSMGFWQSRERYNTQDEILNTVAEFRKRKIGLDNIVLDWRYWREGQ
                     WGSQEFDPARFPDPKGMMKTLHEKYNTNLMISVWAKFYEDTQNYKDLDAKGYILKRNV
                     AENRKDWLGFNNAIYDPYNPDARKEFWKLINKNIYSTGID"
     gene            complement(1692..2723)
                     /locus_tag="PLQ70_10505"
     CDS             complement(1692..2723)
                     /locus_tag="PLQ70_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35088.1"
                     /translation="MKYILLFSTFLTVLTSYSQTNLVKNGGFETELTSWRGDVATISP
                     YDKKSGKNSCVINQFVGSEWKGIDQIITFQKNTAAIEISGWIKTEAIEKGQNIWNTGK
                     FDIEFLNSGEKGIENQSIASVLGTSPWTFYKKIITVPAGASKIRIMLALGQTNGTIFF
                     DDIKAIAITLEQLNKIHEEENAKTTAALISANSDSKPTVFSNGGFENGMNSWRGNVTT
                     STSIFKEGKAALILKSPTMDWTGIDQIADVPENVSSITLSAWLKSDAIKQGKDVWNNG
                     LFNVEFTGNTQQKTGEQNIAFITGTTDWTYFTKTFQLPAGTKKYRIMLALGFASGTLY
                     ADDISVIFK"
     gene            complement(2739..3932)
                     /locus_tag="PLQ70_10510"
     CDS             complement(2739..3932)
                     /locus_tag="PLQ70_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HQE35089.1"
                     /translation="MSQKTKNLKTELTAELDNILEYWSKHTIDSKNGGFIGQIDYKEF
                     KNFEAEKGSVLNARILWSFAAAYSITKDEAHKQLAKQAFEYIITHFYNAEFGGIFWSI
                     NPDKTPKDAKNQIYALAFTIYGMSEYYAISNDERALKYAIALYKKIQKYSYDPINKGY
                     FEAFTHDWQPIEDLRLSDKDANEKKTMNTHLHIVEGYANLYKVWKDKDLQKYIVKLLE
                     VIETHFIDQKTGHLKLFFNENWVEKPDVISYGHDIEAAWLLLQCAEISGDENIIERYK
                     KYAILLTDATFEGIDSKDGGLWYELDPETNTLMAEKHWWPQSELIIGFFNAYQMTNNQ
                     KYLDVVLKNWEFVKQYILDPENGEWIWGINQDYSKIEKDKTGFWKCPYHNSRACIELI
                     HRIKN"
BASE COUNT         1084 a          701 c          713 g         1547 t
ORIGIN      
        1 catcaatacc tgtactgtag atatttttgt tgattaattt ccaaaattct tttcgagcat
       61 cgggattgta aggatcataa atggcgttgt tgaaacccaa ccaatcttta cggttttcgg
      121 ctacgtttct ttttaaaata tagccttttg catctaaatc tttgtagttt tgagtgtcct
      181 catagaattt tgcccagact gaaatcatta aattggtatt gtatttttcg tgaagggttt
      241 tcatcattcc ttttggatct ggaaaacgtg ctggatcaaa ctcttggctt ccccattggc
      301 cttctctcca gtatctccaa tcgagaacaa tattgtctaa tccaattttt ctttttcgga
      361 attcggcaac agtattcaaa atttcgtctt gtgtattgta gcgttcacgg ctttgccaaa
      421 atcccattga ccattttggc atcatagttg cttttcctgt aagagttctg taaccgctaa
      481 ttacttcgtc gatatttttt ccagaaacaa agaaataatc tattttttga cctgcttctg
      541 attcaaaagc gaattgattt ttttgtgctt cagtttttgg ttttgaccaa gtgaaactta
      601 tgaaagattc atttcctaat ggatcccatt cgatttttag tttgtgtttt actccttttt
      661 caagattgat tcgaattaat tcgttggtag cattccatcc ttgtctccat cttgcggtta
      721 ctaatttgtc gtcaatataa acattggtgt atcctgataa ccaaaggttg aattggtgtt
      781 cacccgaaaa atcagattcg aaagagcctt cccattttac aagtgtattt tctaatttaa
      841 ttccttcagg aagctgtttt aatgatggaa tataactgta atcaatataa gattctggac
      901 gagtaagtac tttttcaggg tcttttttat cggtgtatgt tgcagttaac caacctttgt
      961 ctccggcagc agaatacaat cttagcgtag aaattttgtc ataattttga gtatctatcg
     1021 actttgtaag tgaattattg tcccacaaaa tgccataatt tttgctggat accaagaatg
     1081 gaatcgccac ttcggtattg tattgtgaca ataaaacttt ggttcctttg taattgaaag
     1141 ccccattttg atgttgccct aaaccgtaca aggcttcgtc agtttcgccg ttgaaggatt
     1201 gttttacgat ataagattgt tttccatcca atgtggttcc tgtaatcgtt tttccgccac
     1261 cttttgtttc ttgcaagagt gtttttccag tagcgtcttt aaatattatt tctccagttt
     1321 caatagaaac ggttgcattc aaagtttctg tggaaacagt aacactttcg ttggtgcttt
     1381 ttacatcgaa tttggctacg ttgggtttca aatcaactaa catcaagctt tttgtggtat
     1441 caaaagttga agttgggctc gctgtaatgt gcaaaatagc gttcgaaact acttgaattc
     1501 taagaacagc agcgtcgttt tgttgtggct ttttaatttt aatttcaatt ccatcgttca
     1561 attttgttag gttttcttga gccgaaactt gtaaaaaggg tataaggata aataaaaata
     1621 gaaattgtgg ttttagtttt ttcatttgga tagttattta agtaacaatt ttattgagct
     1681 aaagataggt tttatttgaa gattacggag atatcatctg cataaagtgt gcctgaggca
     1741 aagcctaaag ctaacattat tctatatttt tttgttcccg caggcaactg aaatgttttg
     1801 gtaaaatatg tccaatcggt tgttccagtt ataaaagcga tattttgttc gcccgttttt
     1861 tgttgcgtat ttcctgtgaa ttctacattg aataatccgt tgttccaaac atcttttcct
     1921 tgtttgatag catctgattt gagccaagcc gaaagtgtta tggaacttac attttctgga
     1981 acatcggcga tttgatcaat tcctgtccaa tccatagtgg gggattttaa aataagtgca
     2041 gcttttcctt ctttgaaaat actggtagat gttgttacgt tgccacgcca agaattcatt
     2101 ccgttttcga aacctccatt tgaaaatact gttggttttg agtctgaatt tgctgaaatt
     2161 aaagcggctg ttgttttagc attttcttct tcgtgtattt tgttgagttg ttctagtgtt
     2221 atggcaatag cttttatatc atcaaaaaag atggttccat tggtttgtcc taaagccaac
     2281 attatgcgga ttttggaggc accagcagga actgtaatta tttttttgta aaaagtccaa
     2341 ggacttgttc ctaaaacaga tgcaatactt tggttctcga ttcctttctc tcccgaattt
     2401 aaaaattcga tgtcaaactt tcctgtgttc catatatttt ggcctttttc gattgcttct
     2461 gtttttatcc aaccacttat ttcgatagct gctgtgtttt tttgaaacgt tataatttgg
     2521 tcaattcctt tccattccga acctacaaat tgattgatga cacaactatt tttgcccgat
     2581 tttttgtcat agggtgaaat tgttgctaca tctcctctcc aagatgttaa ttcggtttca
     2641 aaaccaccat ttttaacaag attggtttgt gaatagctag ttaagacagt caaaaatgta
     2701 ctgaatagaa ggatgtattt catataataa ttaaatttct agttttttat tcgatgaatc
     2761 aattcgatgc aggctctact attgtgatag gggcatttcc aaaatcccgt tttgtctttt
     2821 tcaattttgg agtaatcttg gtttattccc caaatccatt ctccgttttc ggggtctaag
     2881 atgtattgct ttacaaattc ccagttttta agtacaacat ccaagtattt ctggttattt
     2941 gtcatttgat aagcattaaa aaaaccaatt atcaattcgg attgaggcca ccaatgtttc
     3001 tctgccatca aggtgttggt ttcgggatct aattcgtacc aaagtccgcc atcttttgag
     3061 tcaattcctt cgaaagtggc gtcggttaaa agaatagcgt attttttgta acgttcaatt
     3121 atattttcat cacccgaaat ttcagcacat tgcaataata accaagctgc ttcaatatcg
     3181 tgtccataag aaatcacatc tggcttttca acccaatttt cgttaaaaaa cagttttaaa
     3241 tgtcccgttt tttggtcgat aaaatgggtt tctatgactt cgagtaattt tacaatgtat
     3301 ttttgtaagt ctttgtcttt ccatacttta tataaattag catagccttc gacgatgtgt
     3361 aaatgcgtgt tcattgtctt tttctcgttg gcatctttat cgcttaaacg caaatcttcg
     3421 atgggttgcc aatcgtgagt aaaagcttcg aaatagcctt tgttaatggg gtcgtaactg
     3481 tatttttgaa tttttttata cagtgctatt gcatatttta aagctctttc atcattagaa
     3541 attgcgtaat attcgctcat tccataaatg gtaaaagcca acgcataaat ttggtttttg
     3601 gcatcttttg gtgttttatc aggattgatg ctccaaaaaa tcccaccaaa ttccgcatta
     3661 taaaaatggg ttataatata ttcaaaagct tgttttgcaa gctgtttgtg tgcttcatct
     3721 ttagtgatag aataagcagc agcgaatgac caaagaatcc tagcattgag cacagaacct
     3781 ttttcggctt cgaaattttt gaactctttg taatctattt gaccaataaa accaccgttt
     3841 tttgagtcaa tagtgtgttt cgaccaatat tcaagaatgt tgtcgagttc agcggttaat
     3901 tcggttttaa gatttttagt tttttgagac acgtttgttt ttttataact attggtttat
     3961 agtagcggtc agagactgct aatcacctcc acaaagtcgg agactttgcg gagcaggatt
     4021 tattatttaa tttctttgtt cttgt
//
LOCUS       DAOSNN010000236         4027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_22455_length_4027_cov_4.532226, whole genome shotgun sequence.
ACCESSION   DAOSNN010000236 DAOSNN010000000
VERSION     DAOSNN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22455_length_4027_cov_4.532226"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..889
                     /locus_tag="PLQ70_10515"
     CDS             98..889
                     /locus_tag="PLQ70_10515"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HQE35090.1"
                     /translation="MTKDEIIDTIAAIKKHSSIEYGVKTMAVDFTKKLFSTLFDTNAP
                     LDQSIEELEQLFMSISKLSFNKSDDVNESIWQQFLEKLPSVLVNLNQDAAYILENDPA
                     SNSIEEVYLAYPGFYAIAIYRLSHELYLLNMLLLSRLMSEYAHHTTGTDIHAGATIAS
                     PFFIDHATGIVIGETTVIEKNVKLYQGVTLGALSVSKDMKNTKRHPTVEKNVCIYANA
                     TILGGETTIGKDSIIGGNTWITKSIPAKSIVTNTTITEVKIKELK"
     gene            889..1776
                     /gene="cysM"
                     /locus_tag="PLQ70_10520"
     CDS             889..1776
                     /gene="cysM"
                     /locus_tag="PLQ70_10520"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HQE35091.1"
                     /translation="MQFKKLSDLIGNTPLVESVNLVKNKNVKILLKLEGNNPGGSVKD
                     RAAYNMIASAIERDDIKKGDKLIEATSGNTGIALAMIAQLFNVEIELILPEDSTIERT
                     QTMRAYGATVILTPASEGIIGSRDYADKKVAEGGYVMLNQFANDDNWKAHYKTTGPEI
                     WKDTQGTVTHFVSAMGTTGTIIGTSTYLKEKNPAIQIVGAQPSDGSQIPGIRKWPQEY
                     LPKIFDASKVDVVMEVSETEAREMTKRLAKEEGIFAGMSSGGSVAVALKLAEKLESGV
                     IVAIICDRGDRYLSSDLFD"
     gene            complement(1896..2306)
                     /locus_tag="PLQ70_10525"
     CDS             complement(1896..2306)
                     /locus_tag="PLQ70_10525"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQE35092.1"
                     /translation="MKRHATAVWNGSLKEGAGHLTTQSGILQNTQYSFKSRFEQGIGT
                     NPEELVAAAHSGCFTMQLSAYITEAGFEIESIETKCDINLLEGRIVSSHLTVSAKIKG
                     ISNETFQELVSKAEENCPISKLFDTEISTEASLV"
     gene            2507..3151
                     /locus_tag="PLQ70_10530"
     CDS             2507..3151
                     /locus_tag="PLQ70_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQE35093.1"
                     /translation="MGNKLQLQDWPYLKKYQEDNAKLPLLKTGEQRIVFMGDSITEFW
                     SVHSPEFFIGKPYINRGISGQTSPQMLVRFRADVIALKPTIVVLLAGANDLAGNTGPS
                     TLEMITDNIFSMAELAKVHQIKVILCSVLPAYDFDWKPGSFPAEKIVTLNTMMQQYAE
                     ANEILYLDYHSALVDERKGLDANFTDDEVHPNKTGYEVMGPIVEKAIAEALLSK"
     gene            3162..3641
                     /locus_tag="PLQ70_10535"
     CDS             3162..3641
                     /locus_tag="PLQ70_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35094.1"
                     /translation="MKVLITVLLLFSFNISSYSQVDNFQKKYTSEIIFLKDLNLKNLI
                     KEIIKKDSYCKTEGLDWYIDFMKDDIILISKNSIGNLIAARGINQIYTTTIDDKILFI
                     INAENINDTFIKSGYLSDLSSFIDKSNYSTIDYSFWVIQKNEKNKYKIIKEKIYRCK"
     gene            3767..>4027
                     /locus_tag="PLQ70_10540"
     CDS             3767..>4027
                     /locus_tag="PLQ70_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35095.1"
                     /translation="MLLISCNKIENSKTLDKKDIEYIKSLGLLQDDELIYGFYSQFKN
                     SVAGNFFTNKRVASYWIDKRNANKNEVKFAYYNEIIKIDTVYN"
BASE COUNT         1417 a          676 c          699 g         1235 t
ORIGIN      
        1 atgagtctct ggaataaatt tatttcttga attagtacgg caatttctaa aaacaactta
       61 cttttacctt ttcaactaac atttgaattc taatattgtg actaaagacg aaatcataga
      121 taccattgct gctataaaaa aacattcttc tattgaatat ggagttaaaa ctatggcagt
      181 agattttact aaaaaactat tctcgacatt atttgacacc aatgctcctc tagatcaaag
      241 cattgaagaa ctcgaacagc ttttcatgtc aatttcaaaa ttatctttta ataaatcaga
      301 tgatgtaaat gaaagtattt ggcaacaatt tctagaaaaa ctaccctcag ttcttgtcaa
      361 tttaaatcag gatgctgctt atatcttaga aaacgaccct gcttctaata gcatagaaga
      421 agtttatttg gcttatcctg gattttatgc cattgctatt tatagattaa gtcacgaatt
      481 gtatttgctc aacatgttgc ttttgtcaag gttgatgagt gaatatgcac atcatactac
      541 cggaactgat attcatgctg gagctactat tgcttcacct ttttttattg accatgccac
      601 tggaattgta attggagaaa ccactgtcat cgaaaaaaat gtcaaattat atcaaggtgt
      661 tactttgggc gccttgagcg ttagcaaaga catgaaaaac accaaaagac atccaaccgt
      721 tgagaaaaat gtgtgtattt atgccaatgc cactattttg ggcggagaaa ccaccattgg
      781 aaaagacagc attattggcg gaaacacttg gataaccaaa tcgattccag caaaatctat
      841 tgtaaccaac accaccatta ccgaagtaaa aatcaaagaa ttaaaataat gcaatttaaa
      901 aaattatcag atcttatcgg aaacacacct ttagtcgaaa gcgtgaattt ggtaaagaac
      961 aaaaacgtca aaattttgct aaaacttgaa gggaataatc ccggaggaag cgtaaaagac
     1021 cgtgctgcct ataatatgat tgcatcggca atagaaagag acgacatcaa aaaaggcgac
     1081 aaactcatag aagccacaag cggaaacaca ggcattgctc tagcgatgat tgcccaatta
     1141 ttcaatgtag aaattgaact tatcctgccc gaagattcta caatagaacg cacccaaacg
     1201 atgcgagctt atggtgcaac tgtgatttta actcccgcaa gcgaaggaat tattggatcc
     1261 agagattatg ccgataaaaa agtagccgaa ggcggatatg taatgctcaa ccaatttgcc
     1321 aatgacgaca actggaaagc gcactacaaa actactggcc ccgaaatttg gaaggatact
     1381 caaggaactg taacccattt tgtttcggca atgggaacta caggaaccat aattgggact
     1441 tctacttact tgaaagaaaa aaatccagcc attcaaattg tgggtgctca acctagcgat
     1501 ggttcccaaa ttcctggcat tagaaaatgg cctcaagaat atttacccaa aatatttgat
     1561 gcatcgaaag tcgatgtcgt gatggaagtt tctgaaacgg aagcgcgtga aatgaccaaa
     1621 cgattggcca aggaagaagg catttttgcc ggaatgagca gtggcggttc cgtagctgtt
     1681 gccttaaaac tagccgaaaa actagaatca ggagtgattg tagctattat ttgtgaccga
     1741 ggcgaccgct atttgtcatc agatttgttt gattagtttt tagcttcaat ggcacacgga
     1801 tgaaactgat gtgacagatt tagaccgatt cttattaaaa aaaggctcga tttgttttgc
     1861 aaatcgagcc ttttttactg gatacaaacc tgaatttaaa ccaaactggc ttcggtagaa
     1921 atttctgtgt cgaataattt tgaaatggga caattttcct cggctttact aaccaattct
     1981 tgaaaagtct cgttggaaat tcctttgatt ttggcactta cggttaggtg cgaactcacg
     2041 attctacctt ctaataaatt aatatcgcat ttagtttcga tgctttcaat ctcgaaacca
     2101 gcttctgtaa tataggccga aagttgcatc gtaaaacagc ccgaatgtgc tgcagcaact
     2161 aactcttctg gattagtacc aattccttgt tcaaaacgag atttaaatga atattgggta
     2221 ttttgcaata ttccactttg tgtggttaaa tgaccggctc cttctttgag cgagccgttc
     2281 caaactgcgg ttgcgtgtct tttcataatt ctatttttaa tggttaccca aatttaatca
     2341 tttaaaatcc aatatctttt gacttttcac catttaacta aaatttaaaa tataattcaa
     2401 acaatacttt tcatcactac aattaatagg taatactgga cgaaatgctt atttttgttg
     2461 actgctttat taggatttac taaaaccaga atgtcaattt caccgtatgg gaaacaaatt
     2521 acaactacaa gactggccgt atttaaaaaa ataccaagaa gacaatgcca aactccctct
     2581 tttgaaaaca ggagaacaaa gaatcgtatt tatgggcgat tctataaccg agttttggtc
     2641 ggtacattct cctgagtttt ttattggaaa accgtatata aaccgtggaa ttagcggtca
     2701 aacctcgcct caaatgctag ttcgtttcag ggccgatgta attgctttaa aacccacaat
     2761 tgtggtgtta ttggctggag ccaatgatct tgcaggaaat acaggccctt cgactttaga
     2821 aatgataact gacaacattt tctcgatggc tgaattggcc aaagttcacc aaatcaaagt
     2881 gattttatgc tcggtcttgc ctgcttatga tttcgattgg aaaccgggtt cttttcctgc
     2941 cgaaaaaatt gtgacgctca atacaatgat gcaacaatac gccgaagcca atgaaattct
     3001 atatttggat tatcattcag ctttggttga tgaacgaaaa ggattagatg ctaactttac
     3061 tgacgatgaa gttcacccca ataaaacagg atatgaagtg atggggccga ttgtggaaaa
     3121 ggcgatagcg gaagcgttat tgtctaaata aatatcaaat tatgaaagta ttaattacag
     3181 tactcttgct ttttagtttt aacatttctt cttattcaca agttgacaat tttcaaaaaa
     3241 aatacacctc agaaattatc tttttgaaag atttaaattt aaaaaactta ataaaagaaa
     3301 ttataaaaaa agatagttat tgtaaaacag aaggccttga ttggtacatt gattttatga
     3361 aggatgatat aatattgatt agcaaaaata gtattggcaa tttgatagct gcgagaggca
     3421 ttaaccaaat atatacaact acaatagatg ataaaatttt attcattata aatgctgaaa
     3481 acattaatga tacattcatt aaatctggat acttgtcaga tttaagttct tttatagata
     3541 aatctaatta ctcaaccata gactattcat tttgggttat tcagaaaaat gagaaaaata
     3601 aatataaaat aatcaaggaa aaaatataca gatgtaagta ataatttttt aagtgaattt
     3661 agatatttac actaatcttg agaggttaat aactaaaaaa ccataaaact ttaaaaataa
     3721 aaattaatca gttatgcgga attattttaa tgtaacattc attttattgc tactaatttc
     3781 ttgtaacaag attgaaaata gtaaaactct tgataagaaa gatatagaat atatcaaaag
     3841 tttaggactg ttacaagacg acgagttaat atatggtttt tatagccaat ttaagaacag
     3901 cgttgcaggc aattttttca ctaataaaag agtcgcttct tattggatag ataaaagaaa
     3961 tgcaaacaaa aacgaagtta aatttgctta ttataatgaa attataaaaa tcgatactgt
     4021 ttataat
//
LOCUS       DAOSNN010000237         3980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_22815_length_3980_cov_4.588025, whole genome shotgun sequence.
ACCESSION   DAOSNN010000237 DAOSNN010000000
VERSION     DAOSNN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22815_length_3980_cov_4.588025"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLQ70_10545"
     CDS             <1..753
                     /locus_tag="PLQ70_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE35096.1"
                     /translation="NIFYAVKSKVFAVGYSVTSECNTYNFTTPVAIPDGPATYTTRTI
                     NVPASSASIVDVNVGIGIQHAFMSDVEIEIVSPEATTVVIQKSSCGTNLNINYDDSGN
                     AISCTSTTLQNVIPKQSLSAFNGQNPSNTWTLRFRDASSGGSGTLNTASITICTKTYV
                     ALGTNDLYLTNFEMYPNPSKGQFNIQFSSQSKNDIKVLVHDLLGRKVFENKYKNNSSF
                     NEDIQLKNVTSGIYLLTVVDGDRKEERKIIIE"
     gene            864..1598
                     /gene="rlmB"
                     /locus_tag="PLQ70_10550"
     CDS             864..1598
                     /gene="rlmB"
                     /locus_tag="PLQ70_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQE35097.1"
                     /translation="MEKEHLIFGIRAIIEAIQAGKEVDKVFIQKEISGELMKDLMKVM
                     KRANVNFSYVPVEKLNRLTPNNHQGAVASISPIGFIDLEHLVDATLESGKTPLFLILD
                     QISDARNFGAIIRTAECTGVNGIIVQKAGSAPVNGDTVKTSAGAVFNVPICKVEHIKD
                     AIFYLQGSGIKTVAATEKTDQNIYDIALNEPVAIIMGSEDRGINPSVLKIVDEKAKLP
                     MFGTIGSLNVSVACGAFLYEVVRQRS"
     gene            complement(1632..2417)
                     /locus_tag="PLQ70_10555"
     CDS             complement(1632..2417)
                     /locus_tag="PLQ70_10555"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQE35098.1"
                     /translation="MNPKIEDFNHFKFTPSVWIVPTLMMLLNWFIFYLDNLFHLNLDE
                     HGIYPRTLSGFQGVLFSPFLHGDLNHIASNTVPLFILTTALIYFYRDVSLKVLVYGIW
                     FSGLITWVIGRESYHIGASSLIYVLVSFIFFKGIQTQYYRLVALSLSVILVYGGLVWY
                     IFPKVDETISWEGHLAGLITGFVLSLYYKTPEYKKVIKYDWEHPDFNPMEDKFMQRFD
                     ENGNFVNLPEVEPDEEIQSNYFSNFPIDYTIVESEKNESKPEL"
     gene            2588..3865
                     /locus_tag="PLQ70_10560"
     CDS             2588..3865
                     /locus_tag="PLQ70_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQE35099.1"
                     /translation="MEAPLAERIRPQKLEDYISQSHLVGPNGSLTQQIAKGIIPSLIF
                     WGPPGTGKTTLAQIIAQESKRPFYILSAINSGVKDIRDVIEKAKQSGGLFTSKNPILF
                     IDEIHRFSKSQQDSLLAAVEKGWITLIGATTENPSFEVIPALLSRCQVYILNAFTKDD
                     LESLLHRALKTDNYLSTKNIKLTETEALLRLSGGDGRKLLNIFELVVNASDEDEISIT
                     NDRVFELVQQNTVLYDKSGEQHYDIVSAFIKSIRGSDPNGAVYWLARMIEGGEDVKFI
                     ARRMLILSSEDIGNANPTAFIMANNTFQAVTTIGYPESRIILSQCAVYLATSPKSNAS
                     YLAIGNAQQLVKQTGDLPVPIHLRNAPTKLMKELGYGDEYKYSHDYANNFAEQEFLPD
                     AIKETVLYNPGNNSRENSNREFLKNRWKDKYGY"
BASE COUNT         1449 a          674 c          673 g         1184 t
ORIGIN      
        1 aatatatttt atgcagttaa atccaaagta tttgctgttg gctattctgt aacttctgaa
       61 tgtaacacat acaactttac tactccagtc gcaattcctg atggcccagc aacttataca
      121 acaagaacaa ttaatgttcc ggcatcatct gcatcaattg tagatgttaa tgtaggaatt
      181 ggaattcaac atgcttttat gtcagatgtt gaaatagaaa ttgtaagtcc agaagctaca
      241 actgtagtaa tacaaaaaag tagttgtgga acaaatttaa atattaatta tgacgattca
      301 ggaaacgcta ttagttgtac atcaacaaca ttacaaaatg ttataccaaa acaaagtcta
      361 agcgccttta atggtcaaaa cccatcaaac acttggacat tgagattccg tgatgcaagt
      421 agtggtggtt caggaacatt aaatacagct tccataacta tttgtacgaa aacatatgtt
      481 gcattaggaa caaacgactt gtatttaact aattttgaaa tgtacccaaa tccaagtaaa
      541 ggccaattta acattcaatt ttcaagtcaa tctaaaaacg atattaaggt tttagttcac
      601 gatttattag gtagaaaagt ttttgaaaac aaatacaaga ataactcttc ctttaatgaa
      661 gacatccaat taaaaaatgt aacctcaggc atttacttac ttacagttgt tgatggagat
      721 agaaaagaag aaagaaaaat aataattgaa taattaagac actaaatttt aaaaaagggt
      781 aacactaaaa atgttaccct tttttattgg ttgaaaataa aaaatttaga ttctcgaaat
      841 ataaaaaaac ctatctttgc ccaatggaaa aagaacattt aatattcggg ataagagcca
      901 ttattgaggc tattcaagca ggaaaagaag tcgataaagt atttatacaa aaagaaatat
      961 caggcgaatt gatgaaagac ttgatgaaag taatgaaacg tgccaacgta aatttctctt
     1021 atgttcctgt cgaaaaatta aatcgtttga ctccaaacaa ccatcaagga gcagtagcat
     1081 ccatttctcc aataggtttt attgacttag aacacttagt cgatgctacg cttgaatctg
     1141 gaaaaacacc tttgtttttg attctagatc aaatatcaga tgctcgaaat tttggagcta
     1201 ttattagaac tgctgaatgt acaggtgtga acggaattat tgttcaaaaa gcaggttccg
     1261 ctccagtaaa tggagatact gttaaaactt cggctggtgc agtctttaat gtacctattt
     1321 gcaaagtaga acatatcaaa gatgctattt tctatttaca aggttcagga ataaaaaccg
     1381 ttgcagcgac tgaaaaaaca gaccaaaata tttatgatat tgccttaaat gaacccgttg
     1441 ctattattat gggttcagaa gatagaggaa ttaatccttc tgttcttaaa atcgtagatg
     1501 aaaaagcaaa attacctatg tttggaacca ttggctcact aaatgtttcc gtggcttgcg
     1561 gagctttttt atacgaagtc gttagacaaa gaagttaagt gtttttgatt ttaagtaaaa
     1621 tataaaacga attagagctc tggttttgat tcgttttttt cgctttcaac aatagtataa
     1681 tctataggaa aatttgagaa atagttcgat tgtatttctt cgtcaggttc aacttctgga
     1741 agatttacaa aattgccatt ttcatcaaat cgttgcataa acttatcctc cattggatta
     1801 aaatctggat gttcccaatc gtatttgatt acttttttgt attctggagt tttgtaatat
     1861 aacgaaagta caaaaccagt aatcaatcca gccaaatgtc cttcccaaga aatggtctca
     1921 tccacttttg gaaaaatata ccaaaccaat cctccataaa caagaattac tgacaatgac
     1981 aaagctacta aacggtaata ttgggtttga attcctttaa aaaaaataaa actcaccaaa
     2041 acataaatca aactactggc tccaatatga taggattcac gaccaataac ccaagtaatt
     2101 aacccagaaa accaaattcc atacaccaaa actttcaacg aaacatcccg ataaaaataa
     2161 atcaaagctg tagttaaaat aaaaagcggt acagtattac tagcaatatg attcaaatcg
     2221 ccgtgcaaaa aaggactaaa caaaacccct tgaaaacccg ataaagtcct tggataaatt
     2281 ccgtgctcgt ctaaatttaa gtgaaacaaa ttatccaaat aaaaaataaa ccaattcaac
     2341 agcatcatca atgttggaac aatccaaacc gatggcgtaa atttaaaatg attgaaatct
     2401 tctatttttg ggttcaaggt attgtgtctt aggttttaga ctttgacaat taatagtcaa
     2461 aaacagaacc aaactaaaag taatgctatt ttgtcagaac tcttaatctg gttgttcgag
     2521 tatttgttga tttaaaagta caaataaact aaatttgtga ttcaagaaat caaaactcta
     2581 aattattttg gaagcaccgc tagcagaacg cattcgacca caaaaattag aagattatat
     2641 cagtcagtcg catttagtgg ggccgaatgg ttcgttgacg caacaaattg ccaaaggaat
     2701 tattccgtca ttgattttct ggggacctcc cggaactgga aaaacaactt tggcacaaat
     2761 tattgctcaa gaatcgaaac gtccttttta tattctgagc gccattaatt cgggagtgaa
     2821 agatattcgc gatgtgattg aaaaagccaa acaaagtggc ggacttttta cttccaaaaa
     2881 ccccattctg tttatcgatg aaattcatcg cttcagcaaa tcacaacaag attctttatt
     2941 ggctgccgtc gaaaaaggtt ggataacatt aataggtgcc actaccgaaa atccaagttt
     3001 tgaagtaatt cctgcattat tatctcgttg tcaagtctat attctgaatg cgtttactaa
     3061 agatgatttg gaaagtttat tgcatcgcgc tttgaaaacg gacaattatt taagtacaaa
     3121 aaatataaaa ctcaccgaaa ctgaagcctt attaagactt tcgggtggcg atggacgcaa
     3181 actattaaat attttcgaac tggtcgtaaa cgcttccgat gaagacgaaa tttcaatcac
     3241 aaacgatcga gtttttgaat tggtgcaaca aaacacggtt ttgtatgaca aaagtggcga
     3301 acaacattat gacattgttt cggctttcat caaatcgata cgtgggagtg atcctaatgg
     3361 tgccgtttat tggctagccc gaatgattga aggtggcgaa gatgtaaaat tcattgccag
     3421 acgaatgctc attttatcga gcgaagatat tggaaatgcg aatccaaccg cttttattat
     3481 ggcgaataat acttttcaag ccgtaaccac tattggctat cccgaaagta gaattatttt
     3541 gagtcaatgt gccgtttatt tagccacttc acccaaaagc aatgcctcgt atttagccat
     3601 tgggaacgct caacaattgg ttaaacaaac cggagattta ccagttccta ttcatttgcg
     3661 caatgcccca accaaattaa tgaaagaatt aggttatggt gatgaatata aatattcgca
     3721 tgattatgcc aataattttg ccgagcaaga gtttcttccg gatgctataa aagaaacggt
     3781 tctttacaat ccaggcaaca attcaagaga gaacagcaac cgtgaatttt taaagaaccg
     3841 ttggaaagat aaatatggat attagtttca gaaggcagat agcagaataa aaaaagaaga
     3901 cagactagat gtttaacatt cttaatctga aatctgaaat ctgaaatctg aaatctgaaa
     3961 tctgaaatct gaaatctgaa
//
LOCUS       DAOSNN010000238         3971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_22871_length_3971_cov_4.593207, whole genome shotgun sequence.
ACCESSION   DAOSNN010000238 DAOSNN010000000
VERSION     DAOSNN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22871_length_3971_cov_4.593207"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLQ70_10565"
     CDS             complement(<1..460)
                     /locus_tag="PLQ70_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35100.1"
                     /translation="MKNSITKIATVITLLLCNAFYAQNIITVDNNVNSSANYTSLQTA
                     IDNAQANDYIYLYPSETTYGNATCAKKLHFRGIGHTPEATNGIKGIINVLTFGTNASA
                     SSVAGLELTQINISAVVHNLVIENNKIGYISSDRDADNWVIRGNVIENQII"
     gene            631..1398
                     /locus_tag="PLQ70_10570"
     CDS             631..1398
                     /locus_tag="PLQ70_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQE35101.1"
                     /translation="MKIALIQSTLFWENPQANRNHFEEEINALTEKVDLIVLPEMFST
                     GFTMNPKAVFETMESETILWMQSLAKAKNCAITGSLVIKENDNFYNRLLFVFPSGEIQ
                     TYDKRHLFTLAGEDKVYTAGEEKLIVDYLGWKICPLVCYDLRFPVFARNVEEYDVLLY
                     VANWPKVRIQAWDILLKARSVENMCYTIGVNRVGLDNNNFEHDGHSQVVDFMGNYVLE
                     PQENEGVFVIELNKEKLLEARNRLGFLNDKDFFDLKP"
     gene            complement(1387..3054)
                     /locus_tag="PLQ70_10575"
     CDS             complement(1387..3054)
                     /locus_tag="PLQ70_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQE35102.1"
                     /translation="MLKNNYKYILLLLILVMTSCAKRGSITGGLKDTLAPVVKLSFPK
                     NYSTNFKGTEIKLTFDEYIKLKDLKKQLIISPPLKNEPLILPTSVGKFISIKINDTLQ
                     ANTTYNFNFGQSIEDNNEGNPLNQFKYVFSTGSYIDSLSISGAVKDAYTKDVESFVSV
                     MLYDVNDKFNDSVVYKGTPRYITNTLDSLKTFKLENLKAGKYLLVAMKDYGSNNKFNP
                     SKDKIAFNKQFITIPNDTIFELKLFKEVQPFKAIKPIQASGNRLIFPYEGKADGVKLA
                     LKNKGVDLPIIITKFPKKDSLQVWYKPLKTDSLSLAVSKEKYNKNFTFKFKEQKKDSL
                     NITALQNGVLNFREQFTIESATPLTKFDNSKIKLTNKDSVAVPFTTKYDEYNQRLFFD
                     FKKDFSQNYTFKMLPGAMTDYLEKTNDTLIYRLSTKELEDYGNLKVNLQNVRRFPVMV
                     QLTNQAGDVVATEYTETNTKVEFNLVEPALYLLRAIYDNNKNKEWDSGNYLEKRQAEE
                     VVYLSKEIDIRANWDVEQVFDLSIPYTPEPKKKVDKNKVEKKKKSGF"
     gene            complement(3184..3867)
                     /locus_tag="PLQ70_10580"
     CDS             complement(3184..3867)
                     /locus_tag="PLQ70_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQE35103.1"
                     /translation="MFKSIVNLFFPPVCAGCHSFLVSNENVICTLCRHNLPLTNHHLN
                     PENEAFKKFYGRIPVEHSSAMLYFHKKGIVQELIHNLKYKGQEEIGTVLGEWYAYELK
                     NIETLQSVDEIIPVPLHKRKYRERGYNQLTNFGKTLSKKLNIPYNSNVLVRNIYSKTQ
                     SRKSFLNRSEGIDTIFDVNFSEKDHGKHFLLIDDVLTTGSTLEACSHALLKIPGTKIS
                     IVCMAMAHS"
BASE COUNT         1239 a          645 c          672 g         1415 t
ORIGIN      
        1 tgattatctg attttcaatg acatttcccc ttatcaccca attgtcagcg tctctatctg
       61 aactgatata gccgattttg ttgttttcga ttactaaatt atggactaca gcagagatgt
      121 taatttgtgt tagttccaga ccggcaaccg agctagcaga tgcatttgtt ccaaaagtta
      181 aaacgttaat tattccttta ataccgttag ttgcttctgg agtatgtcca atccctctga
      241 aatgtaattt tttggcacaa gtagcatttc cgtatgtagt ttcagacggg tagaggtaga
      301 tgtaatcatt ggcttgtgca ttgtcaatgg cggtttgcaa tgaagtataa ttggcagacg
      361 aattgacatt attgtccacc gtaataatgt tttgagcata aaacgcattg catagcaata
      421 gcgttataac tgtagcgatt tttgtaattg aatttttcat tggtttactt tttataattg
      481 ctttaaattt ttgatgcaag ttatattcat caaacaattc attcatcagg ggaattgacc
      541 attttaaaaa ttcggggttt tccctgataa ataagttact ttgttgagga taattctatt
      601 ttatgtaact ttgatattat ttgacaaaaa atgaaaatcg ccttaatcca atccacatta
      661 ttctgggaaa atccccaagc caatcgaaac catttcgaag aagaaatcaa tgctcttact
      721 gaaaaagtag atttgattgt gcttccagaa atgttctcaa caggttttac gatgaatcct
      781 aaagctgttt ttgaaacaat ggaaagtgaa actattttgt ggatgcaatc tttggcaaaa
      841 gccaaaaact gtgcgattac aggaagttta gtcatcaaag aaaacgacaa tttctacaat
      901 agattactgt ttgtgtttcc atcaggtgaa atacaaacgt atgataaacg acatttgttt
      961 acgctggctg gcgaagataa agtctataca gctggcgaag aaaaattgat tgtcgattat
     1021 ttaggttgga aaatttgtcc gcttgtatgt tacgatttgc gttttcctgt ttttgctcga
     1081 aatgtcgaag agtatgacgt attactttat gttgccaatt ggccaaaagt aagaattcaa
     1141 gcttgggata ttttacttaa agcccgttcg gtcgaaaata tgtgttatac tattggcgtg
     1201 aacagagtag gtttggacaa taacaatttc gagcacgatg gtcattcgca agtggttgat
     1261 tttatgggga attatgtttt ggaaccacaa gaaaacgaag gtgtttttgt gatagaatta
     1321 aacaaagaaa aactccttga agcaagaaat aggttaggat ttctcaatga taaagatttt
     1381 tttgacttaa aaccctgatt tctttttctt ctccacttta tttttgtcca ctttcttttt
     1441 gggttctgga gtgtatggaa tactcaaatc aaaaacttgt tctacatccc aattggcacg
     1501 aatatcaatt tctttcgaaa gatagaccac ttcttcggct tgacgttttt cgagataatt
     1561 tcccgaatcc cactccttat ttttattgtt gtcataaata gccctgagta aatacaaagc
     1621 aggttctacc aaattgaatt ctacttttgt attggtttcg gtatattctg ttgcaactac
     1681 atcacctgct tgattagtca gttgaaccat taccggaaag cgtctaacgt tttgtaaatt
     1741 cacctttaga ttaccataat cttctaattc tttggtggat aatctataaa ttaaggtgtc
     1801 gtttgttttt tctaaataat cggtcattgc tccaggcagc atcttgaaag tataattttg
     1861 cgaaaaatct tttttgaaat caaaaaacaa tctttggtta tactcatcat atttagttgt
     1921 aaaaggcaca gcaaccgaat ctttgttagt gagttttatc ttcgaattgt cgaattttgt
     1981 caatggtgtt gcactttcta tagtaaattg ttctctaaaa ttcaaaacgc cattttgaag
     2041 agcggttata tttaaactgt cttttttctg ttctttaaat ttaaacgtaa aattcttgtt
     2101 gtatttttct ttagaaactg cgagagaaag tgaatccgtt tttaaaggtt tataccaaac
     2161 ctgaagtgaa tcctttttgg gaaatttagt gatgatgata ggcaaatcga cacctttgtt
     2221 tttcaaggcc aattttactc catctgcttt tccttcatac ggaaaaatca atctgtttcc
     2281 agaggcttga ataggcttta tggctttaaa aggctgtact tctttgaata attttagttc
     2341 aaagatagtg tcgttcggaa tggtgataaa ttgtttatta aaagcaattt tatctttact
     2401 agggttgaat ttattattag aaccataatc tttcatcgcc acaagaaggt atttccccgc
     2461 ttttaaattc tctaatttga aagttttcaa actgtccaaa gtattcgtaa tatatcttgg
     2521 agttcctttg taaactactg aatcattgaa tttgtcattt acatcataaa gcatcaccga
     2581 aacaaaagat tcaacatcct ttgtataagc gtcttttaca gctccagaaa tggaaagcga
     2641 atcgatataa gaacctgtcg aaaacacata tttgaattgg ttgagcggat ttccttcatt
     2701 attatcctca atactttgtc caaaattgaa attataagta gtgttggctt gcaaagtgtc
     2761 atttattttt atgctaataa atttgcccac acttgttggt aaaatcaaag gttcattttt
     2821 caagggaggc gaaataatca attgtttttt taggtctttc aacttaatat attcgtcaaa
     2881 agtcagtttg atttccgttc ctttaaaatt agtgctatag ttttttggaa aactcaattt
     2941 cacaaccggc gcaagtgtat ctttcaaacc gcctgtaata ctccctcttt tagcgcaact
     3001 tgtcattact aatataagta acaacaaaat atatttatag ttgtttttca acataagaaa
     3061 tcaaaattta gaagctacaa aataacaatt atattcgctg taatcgaaat ttttatgaaa
     3121 ttattagtct ttttagacct tacaggtttc aaaaacgggt taggtcttgt ttgactggaa
     3181 aaatcaagaa tgcgccatgg ccatacagac aatgctgatt tttgtccctg gaatttttag
     3241 taaagcgtga gagcaagctt ctaaagtcga gccggtggtc aatacatcat caatcaataa
     3301 aaaatgtttg ccgtgatctt tttcggaaaa attaacatca aaaatggtgt ctataccttc
     3361 gcttcgattc aaaaaacttt ttcgagattg ggttttggag taaatattcc taaccaaaac
     3421 attagagtta taaggaatat tcagtttttt agaaagtgtt ttcccaaaat ttgtcaactg
     3481 attgtagccc ctttctctgt attttctttt atgcaaaggt acaggaataa tttcatcgac
     3541 agattgaagt gtttctatgt ttttcaattc ataagcgtac cattcgccca aaactgttcc
     3601 aatttcttct tgacctttgt atttcagatt gtgaatcaat tcctgaacaa ttcctttttt
     3661 atgaaaatat aacattgccg aagaatgttc gacaggaatt cgaccataaa actttttgaa
     3721 agcctcgttt tctggattca aatgatgatt ggtcaacgga agattatgcc tgcaaagggt
     3781 acaaatcacg ttttcattcg atactagaaa cgaatgacaa ccagcacaaa cgggaggaaa
     3841 aaagagattg acaatgcttt tgaacataaa aaagccaaat ttttataaaa atagcaaaat
     3901 caatgcttca aactttaaaa atgtttcttt ttttaagttc actttttata tttttgcacc
     3961 actatggcaa a
//
LOCUS       DAOSNN010000239         3925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_23199_length_3925_cov_4.556072, whole genome shotgun sequence.
ACCESSION   DAOSNN010000239 DAOSNN010000000
VERSION     DAOSNN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23199_length_3925_cov_4.556072"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..874)
                     /locus_tag="PLQ70_10585"
     CDS             complement(77..874)
                     /locus_tag="PLQ70_10585"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQE35104.1"
                     /translation="MNETLKRAISGAIYIILLLSSILYSTESFFILFGIFLVIAIYEF
                     CNLIQTHKLFPILFGTLLYIAITLVSHYNKITTNTVNTFFNTDFEIPVNIQELDIVLL
                     VVSLVVSIKCILFLFYDKFQKISTSSKYLYLLGYIILPFIFITKISFGINDYNPKIII
                     GLFILIWTNDTFAYIVGKSIGKNKLYEKISPKKTIEGFIGGIIFAILAGYLISKYYIK
                     ASPQFSDKSIMIWTSIALIVGVAGTIGDLIESKFKPGVAVPYAKLFS"
     gene            complement(876..1478)
                     /locus_tag="PLQ70_10590"
     CDS             complement(876..1478)
                     /locus_tag="PLQ70_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B/C"
                     /protein_id="HQE35105.1"
                     /translation="MSLFKKFFGSNNPDSEEGKNKENIDFSLENDAPLEEQFIYNFKK
                     NGGKFLYCENESEVKDHFENILEENDWFEGEALCYEPNLFGILEENKIVFQNANNPSF
                     LLANCENLIADEGSILFSSKQIKQNKPNELPTNIVIIATTSQIIETKSDGLSVIKKKY
                     IKDYPTNITTIKYFKKVVEEDFTQYGSIAKNLYLLLLEDL"
     gene            complement(1591..3516)
                     /gene="ftsH"
                     /locus_tag="PLQ70_10595"
     CDS             complement(1591..3516)
                     /gene="ftsH"
                     /locus_tag="PLQ70_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQE35106.1"
                     /translation="MAKDNKPNSNNFKVSPWLVYTAILLIFLTISFITGGTNFQEPAQ
                     LKSSDIDNMLSKGEIKNVIIFNNKDAEIYLTDAALKDPANKKVAKNVLDQPNKGPHYT
                     TKFGDLKSFQEKLDKAKSEKKLVDYDFKDASNWSDIIISLLPIIIIIGVWIFIMRRMG
                     GGAGGGGGQIFNIGKSKAKLFDEKTDIKTSFKDVAGLEGAKEEIQEIVEFLKNPEKYT
                     NLGGKIPKGALLVGPPGTGKTLLAKAVAGEAQVPFFSLSGSDFVEMFVGVGASRVRDL
                     FKQAEEKSPAIIFIDEIDAVGRARGKNNMSGGNDERENTLNQLLTEMDGFGTNSNVIV
                     LAATNRADVLDKALMRAGRFDRQIFVDLPDIREREEIFKVHLAPLKKVENLDTDFLAK
                     QTPGFSGADIANVCNEAALIAARNNKPAVDKQDFLDAVDRIIGGLEKKNKIITAEEKK
                     AIAIHEAGHATVSWMLEHAAPLIKVTIVPRGQSLGAAWYLPEERQIVRTDQMLDEMCA
                     TMGGRAAEKVTFNKISTGALSDLEKVTRQARAMVTIYGLNEKIGNVTYYDSSGQSEYS
                     FSKPYSDETAKTIDEEISILIEGQYERAIKILEENKDKLNQLADILIEKEVIFKDDLE
                     NIFGKRTFDSNLGEVVS"
     gene            complement(3532..3903)
                     /gene="rsfS"
                     /locus_tag="PLQ70_10600"
     CDS             complement(3532..3903)
                     /gene="rsfS"
                     /locus_tag="PLQ70_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQE35107.1"
                     /translation="MTKKAINNDVLLANIIKGIEEVKGNDIDILDLREIDTAVCDYFV
                     ICNGNSNTQVNAIVNSIQKVVSKELKDKPWHVEGSDNAEWVLMDYVSIVVHVFQKHIR
                     EYYNIESLWGDAKITKIENKY"
BASE COUNT         1229 a          669 c          585 g         1442 t
ORIGIN      
        1 aatctaaaga aggactcata acatttgaat cgaatccgtg gccttcgaat tctatcgaaa
       61 tagatagcca gtcctattat gaaaataatt ttgcatatgg gacagcgact ccaggtttaa
      121 atttggattc aataaggtct ccaatagttc cagcaactcc aacaattaat gctatcgaag
      181 tccaaatcat tatggatttg tcgctaaatt gcggactagc ttttatgtaa tatttggata
      241 ttaaataccc tgctaaaatg gcaaaaataa ttccaccaat gaatccttct attgtttttt
      301 ttggtgaaat tttttcatat aatttgtttt ttccaatcga ttttccaacg atataggcaa
      361 aggtgtcatt tgtccaaatt agtatgaata acccaatgat aatttttgga ttataatcat
      421 tgataccaaa cgatattttt gttataaata tgaaaggtaa gatgatgtat cctaataaat
      481 ataaatattt tgaagaggta cttatttttt gaaatttatc ataaaataaa aatagaatac
      541 attttataga aactaccaag gacaccacta ataatacaat atctaattct tgtatgttta
      601 caggaatctc aaaatcagta ttgaaaaacg tattgacggt attggttgtg attttattgt
      661 aatggcttac taatgttata gcgatatata ataaagttcc aaaaagaata ggaaaaagtt
      721 tgtgagtttg aattaaatta caaaattcat aaatagcaat tactaaaaat attccaaaca
      781 agataaaaaa actttcagtc gaatatagta tcgatgatag taataaaatt atataaatcg
      841 caccagatat tgctctcttg agtgtttcat tcatcttata aatcttctaa aagcaataaa
      901 tagagatttt ttgcaatact accatattgt gtaaaatctt cttcaaccac ttttttgaaa
      961 tattttatag ttgttatatt cgtaggataa tcttttatgt atttcttttt aattacgctt
     1021 aaaccatcac tttttgtttc aattatttga ctagtagttg caataatcac aatatttgtt
     1081 ggaagttcgt taggtttatt ttgtttgatt tgtttggaag aaaataatat cgaaccttca
     1141 tcagcaatta gattttcgca atttgctaaa agaaaggaag ggttatttgc attttgaaaa
     1201 acaattttat tttcttcaag aatgccaaaa aggttaggct cataacacaa agcttcgcct
     1261 tcaaaccaat cgttttcttc gagaatgttt tcaaaatggt cttttacttc tgattcattt
     1321 tcgcagtaca aaaatttacc tccattcttc ttgaaattat agatgaattg ctcttctaaa
     1381 ggggcatcat tttctaggga aaaatcaatg ttttccttat tttttccttc ttcagaatcg
     1441 gggttatttg aaccaaaaaa ttttttaaaa agactcatac tttttctatg ctacttgata
     1501 aactaattga aaacgtccaa agataaaaaa atcttaattc aaaaggcact tttgaattaa
     1561 gattttaaaa taatatgatt ttttattgaa ttaagagact acttcaccta aattagagtc
     1621 aaaagttctt tttccgaata tgttttctaa atcatcttta aaaataactt ctttttcaat
     1681 cagaatatcg gcaagttgat ttaatttgtc tttgttttct tctaaaattt tgatggctct
     1741 ttcatattga ccttcaataa gaatcgaaat ttcctcatca attgttttag cagtttcatc
     1801 agaatacggt ttagaaaaac tgtattcact ttgccccgac gaatcataat aagtaacatt
     1861 tccaattttt tcattcaagc catatatggt taccatagca cgtgcttgac gagtaacttt
     1921 ttctaaatca cttaatgcgc cagttgaaat tttattaaaa gttactttct cggcagctct
     1981 tccgcccatt gtagcacaca tttcgtctag catttggtca gttctcacga tttgtctttc
     2041 ttcaggaaga taccaagcag ctcctaaact ttgtccacga ggaacaatgg ttactttgat
     2101 tagaggtgca gcgtgttcta gcatccagct tacagtggcg tgacctgctt cgtgaatggc
     2161 aattgctttc ttttcttcgg cagtaattat tttgtttttc ttttctaaac ctccaataat
     2221 tctatccaca gcgtcaagaa agtcttgttt atctactgca ggtttattat ttcttgcagc
     2281 aattaaagca gcttcgttac aaacattggc tatgtcagca ccagaaaaac ctggagtttg
     2341 tttggctaaa aagtcagtgt caagattttc cacttttttt aatggagcaa ggtgaacttt
     2401 gaaaatttct tcacgctcgc gaatgtctgg taaatccaca aaaatttgtc tgtcaaaacg
     2461 acctgcacgc atcaaagctt tgtctaaaac atcggctcta ttggttgcgg ctaaaacgat
     2521 tacattcgaa ttggttccaa aaccatccat ttcggtaagt aactggttta aggtgttttc
     2581 tcgttcgtca tttccacctg acatattgtt ttttcctcta gctctaccta cggcatcaat
     2641 ttcatctata aaaatgatgg caggagattt ttcttcggct tgtttgaaca aatcacgaac
     2701 acgtgaagca ccaactccaa caaacatttc aacaaaatca gaacctgata aggagaaaaa
     2761 aggtacttga gcttcgcctg ctacagcttt tgctaataat gttttacctg ttcctggagg
     2821 gccaacaagt aaggctcctt ttggaatttt ccctcctaaa ttggtatatt tttctgggtt
     2881 tttaaggaat tcaacaattt cttgaatttc ttctttagca ccttctaaac cagcaacatc
     2941 tttgaatgag gtttttatat ctgttttttc atcgaatagt tttgctttcg attttccgat
     3001 attgaaaatc tgtccgccac caccaccagc gccgccaccc attcttctca tgatgaatat
     3061 ccaaactcca atgatgatga taattggaag aagacttatg atgatatcac tccaattgct
     3121 agcatcttta aaatcataat cgactaattt tttttcagac ttggctttat ctaatttttc
     3181 ttgaaatgat ttaagatctc caaattttgt agtataatgt ggtcctttgt taggttgatc
     3241 caaaacattt ttggctactt ttttattggc aggatctttt aatgctgcat ccgttaggta
     3301 gatttcggca tctttattat taaaaataat gacatttttg atttcgccct tagatagcat
     3361 gttatcaata tcagacgatt ttagttgggc aggttcttga aaattagtcc caccagtaat
     3421 aaaacttata gtcaagaaaa ttaataaaat tgcagtataa acaagccaag ggcttacttt
     3481 aaaattattc gaatttggtt tattgtcttt agccattacg ggtatatttc tttaatattt
     3541 attttcgatt ttagtgattt tggcatctcc ccaaaggctt tcgatgttgt aatactctcg
     3601 gatgtgcttt tggaaaacgt gaaccacaat gctaacatag tccataagaa cccattcggc
     3661 attgtcagat ccttcaacat gccaaggttt atctttcaat tcttttgaaa ctactttttg
     3721 aatggagtta acaattgcgt taacttgtgt atttgaatta ccgttgcaga ttacaaaata
     3781 gtcacaaacg gcggtgtcta tttctcttaa atccaaaata tcgatatcat tcccttttac
     3841 ttcttcaatt cctttgatga tgtttgccaa aagaacatca ttatttattg ctttttttgt
     3901 catgaattat ttttaaataa gtttg
//
LOCUS       DAOSNN010000240         3919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_23239_length_3919_cov_5.136646, whole genome shotgun sequence.
ACCESSION   DAOSNN010000240 DAOSNN010000000
VERSION     DAOSNN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23239_length_3919_cov_5.136646"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLQ70_10605"
     CDS             <1..580
                     /locus_tag="PLQ70_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HQE35108.1"
                     /translation="VSASGVGIYGAKNGSEICTENSSFANDFLGTTCQKWEAASDTIA
                     SLGIRTVKIRTGLVLGKNEGFLKQLVPVFKLKLGSALGSGKQYMPWIHIDDLCGIYLK
                     SLNNSEIKGAYNAAVLDNTTNTVFSKTLAKLFGYSMWLPNVPAFIMQLILGEMAQIVL
                     TGQRVSSNKIESSGFKFQHTSLEEALRDCVLK"
     gene            complement(558..1121)
                     /locus_tag="PLQ70_10610"
     CDS             complement(558..1121)
                     /locus_tag="PLQ70_10610"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQE35109.1"
                     /translation="MKKILFIVFLFAVKNSFAQKEIATTMGLVYFEASVPLFEEVAAT
                     NKTVSCVLNLKTGEITSVVKMNEFRFKLSLMEEHFNQKYLETNYFPNATLKGKIEGFN
                     INIIDENFKEFKLNGVLKIHGKSKKISILVKVKNLNKTIEISSNFNLSVKDFDIKIPE
                     ILSMKVAEVAKIQTLFVFEKPILAHNP"
     gene            complement(1192..1557)
                     /locus_tag="PLQ70_10615"
     CDS             complement(1192..1557)
                     /locus_tag="PLQ70_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HQE35110.1"
                     /translation="MKKILIIITAILTLVLICYFAFLYNATYSEGVRSGELIKFSSKG
                     IIFKTYEGELSQGISGAQIFSFSVLDSEEKVISDLKELEGHYVKLTYIERYKTFPWWG
                     DSKYFIIGVQKEESPFKIK"
     gene            complement(1570..2928)
                     /gene="rmuC"
                     /locus_tag="PLQ70_10620"
     CDS             complement(1570..2928)
                     /gene="rmuC"
                     /locus_tag="PLQ70_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQE35111.1"
                     /translation="MSNMLSFLLLFIVALAIGIFIGKLIFSARFQSEKISLEQKLIAL
                     NNQFDQQKEQFLLDKTAFEKQLELANSEKENIRNEKDSLAIQLSKKEVDFDNLWERNK
                     EQKEEVEKLQEKFTKEFENLANKILDEKSNKFTEQNKENMKNILSPLQDKIQLFEKKV
                     EDTHKESIDYHAALRQQILGLKEMNLQMSKETLNLTKALKGDSKMQGNWGELILERVL
                     EKSGLEKDREYFVQQSHTNAEGNRVFPDVVINLPDGKKMIVDSKVSLTAYEKYINEED
                     DDLKNGFLKEHVNSIKRHVEQLGDKNYQDLYQIESPDFVLLFIPMEPAFALALNEDTT
                     LYNKAFEKNIVIVTPSTLLATLRTIDSMWTNQKQQENAFEIARQAGALYDKFEGFVAD
                     LIKIGKKIDESKVEYQGAMNKLVDGSGNLIKRVENLKKMGAKTQKSLPANILTRADKD
                     EI"
     gene            3034..3606
                     /locus_tag="PLQ70_10625"
     CDS             3034..3606
                     /locus_tag="PLQ70_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQE35112.1"
                     /translation="MSEHRHFILHKPYGYLSQFIYELKRKKKLLGELHDFPAGTMAIG
                     RLDEDSEGLLLLTTDGKVSEQVRSKKVDKEYYVQVDGVITQEAIDMIKEGVEIGFDGG
                     KYKTKKCESRLITEIPDFGPRGKKIRDERHGPTSWASITVNEGKFRQVRKMTAAVGFP
                     TLRLVRVRIGNVYLNDLKAGEVREIMNYEL"
     gene            3672..>3919
                     /locus_tag="PLQ70_10630"
     CDS             3672..>3919
                     /locus_tag="PLQ70_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQE35113.1"
                     /translation="MLNIVLVEPEIPNNTGNIGRLCVGTESRLHLIHPFGFVINDKNL
                     KRSGLDYWVHLDVTEYQNVEEWISQIPDKSRVFLMSSHA"
BASE COUNT         1209 a          636 c          685 g         1389 t
ORIGIN      
        1 cgtttcagct tcaggagttg gaatatacgg agctaaaaat ggttctgaaa tttgtacaga
       61 aaacagttcg tttgccaatg attttttggg aacgacttgt caaaaatggg aagcagcttc
      121 tgataccatt gcgagtttag gcattcgaac tgtaaaaatt cgaactggat tggttttagg
      181 aaaaaacgaa gggtttttga aacaactggt tcctgttttt aaattgaaat tgggttcggc
      241 tttaggttca ggtaagcaat acatgccttg gattcatatt gacgatttgt gtggaattta
      301 tctcaaatca ttaaacaatt cagaaattaa aggtgcttac aatgcagccg ttttggataa
      361 cacaactaac accgttttct caaaaacctt agcaaaacta ttcggttatt cgatgtggtt
      421 gcctaatgtt cctgctttta taatgcaatt gattttggga gaaatggctc aaattgtatt
      481 gacaggacaa agagtttctt caaataaaat tgaaagttca ggtttcaaat ttcaacatac
      541 ttctttagaa gaagcactca gggattgtgt gctaaaatag gtttttcaaa cacaaaaagg
      601 gtttgtattt tcgctacttc tgccactttc atacttaaaa tttcaggtat tttgatatcg
      661 aaatctttaa cagaaaggtt gaaattggag ctaatttcaa ttgttttgtt tagatttttt
      721 accttgacta agatgcttat tttttttgat tttccgtgaa ttttcagtac tccatttagc
      781 ttgaattctt tgaaattttc atcaatgata ttgatgttaa atccttcgat tttacctttt
      841 aaagtggcat ttggaaagta atttgtttcc aaatattttt ggttaaaatg ctcttccatc
      901 aaagaaagtt tgaatcggaa ttcattcatt ttaacaacac tggttatttc acctgttttt
      961 agattaagaa cacaagaaac cgttttgttg gtagcagcaa cttcttcgaa aagtggtaca
     1021 gaagcctcaa aataaactaa tcccatagtg gttgctattt ctttttgagc aaaagaattt
     1081 ttaactgcaa aaagaaacac tatgaaaagt atttttttca ttttgactgt gtaaagaatt
     1141 atctaaattt acaaaattaa tcagacaatt tcatgtagat tgtgtagttt tttattttat
     1201 tttaaatggg gattcttctt tttgaactcc gatgataaaa tactttgaat ctccccacca
     1261 aggaaaagtt ttgtagcgtt caatgtaagt cagttttacg taatgacctt ccagttcttt
     1321 caaatcagaa attacttttt cttcgctatc caacaccgaa aaactaaaaa tttgtgctcc
     1381 cgaaattcct tggcttagtt caccttcata ggttttgaaa atgattccct tgctgctaaa
     1441 ttttatcaat tcgcctgaac gaacaccttc gctgtaagtt gcattgtata aaaaggcaaa
     1501 atagcaaatt agtactaatg ttaaaatagc agtaataatg attaaaattt ttttcatttt
     1561 gtttttggtt taaatttcgt ctttatcggc tctggttaat atgtttgctg gaagtgattt
     1621 ttgagtttta gcccccattt tttttaaatt ttcaaccctt tttatcaagt ttccgctgcc
     1681 atctaccaat ttgttcatag caccttggta ttcgactttg ctttcgtcaa tcttctttcc
     1741 aattttgatt aaatcggcca caaacccttc aaatttatca tacaatgcac cagcttgacg
     1801 ggcaatttca aaagcatttt cttgttgttt ttgattagtc cacatactgt caatagttcg
     1861 taaagttgcc aatagggtag aaggcgttac aatcacaata tttttctcga aagccttgtt
     1921 gtataaagtc gtatcttcat tcaatgccaa agcaaaagcc ggctccatcg ggataaaaag
     1981 caagacgaaa tcgggacttt ctatttggta taaatcctga taatttttat cccccaattg
     2041 ctcaacgtgg cgtttgatcg aattgacgtg ttctttcaaa aagccatttt tcaagtcatc
     2101 gtcttcctcg ttgatatatt tttcataagc ggttaaggaa actttcgaat cgacaatcat
     2161 ctttttgcca tcgggaagat tgatgacaac gtctggaaaa actctatttc cttctgcatt
     2221 ggtgtgggat tgttgcacaa aatattctcg gtctttttct aatcctgatt tttctaaaac
     2281 tctttccaga atgagttctc cccagtttcc ttgcattttg ctgtcgcctt ttagtgcttt
     2341 ggtcaagttc aaggtttctt tgctcatttg aaggttcatt tcttttaaac ctagaatttg
     2401 ttggcgcaaa gctgcgtgat aatcaatgct ttctttatga gtgtcttcga ctttcttctc
     2461 gaacaattga attttatctt gtagaggtga caagatgttt ttcatatttt ctttattctg
     2521 ttctgtaaat ttattcgatt tttcgtctag aattttatta gctaaattct caaattcttt
     2581 ggtaaatttt tcttgaagtt tttctacttc ttccttttgt tctttgttgc gttcccaaag
     2641 attgtcaaaa tcaacttctt ttttagaaag ttgaatggca agactgtctt tctcatttcg
     2701 gatgttttct ttttcagaat tggctaattc caattgtttt tcgaaagcag ttttatcgag
     2761 taaaaattgt tctttttgtt ggtcgaattg gttgttcagt gcaattaatt tttgttccag
     2821 acttattttt tcggattgaa aacgagctga aaaaataagc tttccaatga aaattccaat
     2881 ggcgagcgca acaataaaaa gcaataaaaa cgaaagcata tttgacataa aaagtaaggt
     2941 ttttgtagaa agtaaaagta aggaattata tcaagttaaa attgtatcag aagataattt
     3001 gaaaaatgta tttttgcctt tcaattaaaa ccaatgtcag aacaccgtca tttcatcctt
     3061 cacaaacctt acgggtattt aagtcaattt atttatgagt tgaaacgcaa gaaaaaactt
     3121 ttgggtgaat tacacgattt tcccgcagga acaatggcta ttggtcgcct agacgaagac
     3181 tccgaaggct tacttttgtt gacaactgat gggaaagtaa gtgaacaagt tcggtctaaa
     3241 aaagtcgata aagaatatta cgttcaagtg gatggagtta tcacacaaga agcgattgat
     3301 atgataaaag aaggtgtcga aataggtttt gatggcggaa aatacaaaac caaaaaatgc
     3361 gaatccagac taattactga aattccagac tttggaccga gaggaaaaaa aataagggac
     3421 gaacgtcacg ggccaacctc ttgggcttcc atcacggtta acgaaggtaa gtttcgacaa
     3481 gttcgaaaaa tgacggcagc agttggtttt cctactttaa gattagtaag agttcgaata
     3541 ggaaacgtat atttgaatga tttaaaagcg ggagaagtta gagaaattat gaattatgag
     3601 ttatgagttt tttcaataat aaattttaat tgcaattacc taaaacccaa aacccaaaac
     3661 ccaaaacaag aatgttaaac atcgttttag tagaacctga aatccccaac aatacaggaa
     3721 atattggtcg tttgtgcgta ggtacagaaa gtcgtttgca cctgattcac ccttttgggt
     3781 ttgtcattaa tgataaaaac ttgaaacgtt ctggactcga ttattgggta catttggatg
     3841 taaccgaata tcaaaatgta gaagaatgga ttagtcaaat tccagataaa tcccgtgtat
     3901 ttttgatgag ttcccacgc
//
LOCUS       DAOSNN010000241         3918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_23249_length_3918_cov_3.829148, whole genome shotgun sequence.
ACCESSION   DAOSNN010000241 DAOSNN010000000
VERSION     DAOSNN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23249_length_3918_cov_3.829148"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..648)
                     /locus_tag="PLQ70_10635"
     CDS             complement(184..648)
                     /locus_tag="PLQ70_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35114.1"
                     /translation="MSEIQFNIQQEFREKQEKYVYYLIAISVSAIGFSVYKTENHELN
                     ITLIPLAFAVICWSISIFLGLKFLKYVISVLYANNGYFDIIKGKHPDLGNHPELIKAG
                     VEGFKEAMQINSKRMKKYFNWQGYLFYSGIIFFLIWHILEMYLRTLCQMSCH"
     gene            complement(843..1607)
                     /locus_tag="PLQ70_10640"
     CDS             complement(843..1607)
                     /locus_tag="PLQ70_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HQE35115.1"
                     /translation="MNQKELKSFLDEKVLQYNTLDFIESDPVQIPHLFSQKEDIEIAG
                     FLSATIAWGNRKMIIKNAHRMMDLMGNSPYDFVMSHSEDDLERLESFVHRTFNGQDFA
                     SFIKGLQHIYKNHNGLEAVFAQHQEPNSMQKSISEFKKIFFEIEHQNRAQKHISDPMN
                     NSAAKRINMYLRWMCRQDNKGVDLGIWKSISPSLLSCPLDVHSGNVARKLGLLTRKQN
                     DGKALAELDLKLRELDPNDPVKYDFALFGLGVFEGF"
     gene            complement(1684..>3918)
                     /locus_tag="PLQ70_10645"
     CDS             complement(1684..>3918)
                     /locus_tag="PLQ70_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HQE35116.1"
                     /translation="RFNFKTKKALIWNSKTDQGEFKVKAVITKKENDSVYFLKKARFT
                     TSTNVENPEYYFQTNKVKIIPGKKVVTGLTNLVIANVPTPIALPFAFFPMTKETSVSG
                     ILLPSYNDSNNRGFSLQNGGYYFALSDNYDLTVLGDYYTNGSYALRFESSYAKRYSFR
                     GNLNFRFENLINSERGYPDYSKQSIYNLQWSHSKDSKGSPNSTFSASVNMGSSKYFQQ
                     SINQSNVGSNLNNTLSSSVSYSKTFPGTVGSRLALTATHSQNTQTSEINMTLPDLQYS
                     VDRIYPFVGKDGVKKGFFKNINLLYNLSGKNSFVTTDSLFFTKEMFDNAKLGVQHSIP
                     LSTNFKLFKHFSASTSMNYNEVWTIKTIRRNYDKEQSAVVDQTVNGFDSFRTYNFTSS
                     LGTTIYGTFNFKPDSKIKSIRHVMRPSISYGYTPSFEKYYDTYATDATGKMTKDYTRF
                     EGGVFGAPGNSNSNIVGFDLSNTFEAKITDPDSTKVEPKKIMLLNNLNFATSYNFNAD
                     GKTTFAWAPIRVSGGTQLFKDKLNMNFGATLDPYAIDNAGNTINVYNINNGGSLFRMT
                     SANMTLNYSISSRDSDKDEKDKDKNKQGQRNGGRDDDLFGRSTMDHARGSSQFDDEED
                     TGTDKITEFFASKLPWDMTFAYSLTYGNNNRQNKITGNSIMISANGDLTPKWKVGVST
                     GYDFVLNGVTYTQLRFERDLLSWRMDFNWQPFGDQANWSFFIGIKSGILSDIKYDKRS
                     ASNR"
BASE COUNT         1202 a          688 c          645 g         1383 t
ORIGIN      
        1 cagtttgttt cacagattgt tcattcgttt catcaaattg acgcctttgg aatacaggaa
       61 cagttacaaa tcgatctaat acgaattaat tcaattgtcg ttgactttag aatttgccaa
      121 atcattccaa atacaaaacc aacttctcat taagacgata gcacaaatct gttgtcttag
      181 atgttagtgg caggacattt gacataaagt tcttaaatac atttctaaaa tatgccaaat
      241 caaaaaaaat attattccag aataaaatag atagccttgc caattaaaat attttttcat
      301 ccgtttacta tttatttgca ttgcttcttt aaaaccttca actccggctt taattaattc
      361 aggatgattt ccaagatctg gatgtttgcc tttgattatg tcaaaataac cgttgtttgc
      421 atataatacg ctaattacat attttaaaaa tttaaggcct aaaaaaatac ttatactcca
      481 gcaaatcacg gcaaaagcta atggaattag agtaatgttt aattcgtgat tttcagtctt
      541 ataaactgaa aatcctatag ctgatacaga aattgcgata agataataaa cgtatttctc
      601 ttgcttttct ctaaactctt gctgaatgtt aaattgtatt tcactcattt gccatttctt
      661 ttagttttgt cttgccacta acacatttat tgacgagaca aacctttata tttacacgca
      721 agtttagata gattgtcgaa ggtttgtttc gctttatagt ttgtcaaata tataaataat
      781 tatttacaaa tcatcaaaag tactttttga ttgtaaatat ttaaaaatct ctgtgatatc
      841 tatcaaaatc cctcaaaaac gcccaatcca aacaaggcaa aatcatattt cacgggatcg
      901 ttcgggtcga gttcacgaag ttttaaatca agttctgcca aagctttgcc gtcgttttgt
      961 tttcttgtta gcaagcctaa ttttcgagcc acattgccgg aatgcacgtc taaaggacaa
     1021 gataggagcg aaggcgaaat gcttttccaa atgcctaaat caacgccttt gttgtcttgg
     1081 cgacacatcc atctcaggta catgttgatg cgtttcgccg cggaattatt cattgggtcg
     1141 gaaatgtgtt tttgggctcg gttttgatgt tcaatttcga agaatatttt cttgaattcg
     1201 gaaatgcttt tttgcattga attcggttct tggtgttggg caaaaacagc ttcaagacca
     1261 ttgtggtttt tataaatgtg ttgcaaacct tttataaaac tggcaaaatc ctgtccgttg
     1321 aaagttcggt gaacaaaaga ttccaatcgt tccaaatcat cttcggaatg tgacatcaca
     1381 aaatcatacg gcgaattgcc catcaaatcc atcattctgt gggcgttttt gataatcatc
     1441 ttgcgattgc cccaagcgat ggtagcgctc aaaaaaccgg caatttcgat gtcttctttt
     1501 tgcgaaaaca aatgcggaat ttgcacggga tcactttcga taaaatcgag cgtgttgtat
     1561 tgcaggactt tttcgtcaag gaaggatttg agttcttttt ggttcatctg aagattcaat
     1621 tttgaaaaca ggactttatt ttacctaaat tgacactaaa ttttgcaata aaagacattt
     1681 taattatcta ttgctagcgc ttcgcttgtc atattttata tcgcttaata tgcctgattt
     1741 tattccaata aagaaactcc aattggcttg atctccaaag ggttgccagt taaaatccat
     1801 tcgccaactc aataaatctc tttcaaaacg aagttgggta taagttactc cgtttagcac
     1861 aaaatcataa ccagttgaga caccaacttt ccattttggg gttaaatctc cattggcaga
     1921 aatcattatt gaatttcctg taattttatt ttgtcggtta ttgttgccat aagtaagaga
     1981 ataggcaaat gtcatatccc aaggcaattt tgaggcaaaa aattcggtaa ttttatctgt
     2041 tccggtatct tcttcatcat caaattggct gcttcctctg gcgtgatcca tggtgcttct
     2101 tccaaacaaa tcatcatcac gaccaccatt tctttgacct tgcttgtttt tatctttatc
     2161 tttttcatct ttatcagagt ctctgctgga aatagaataa ttcaaagtca tattggcact
     2221 tgtcattcta aacaaacttc caccattatt gatgttataa acattaattg tattgcccgc
     2281 attatctatt gcataaggat ctaaagtcgc tccaaaattc atatttagct tgtctttaaa
     2341 tagttgagtt ccgccgctta cacgaatcgg agcccaagca aatgtagttt tgccatcagc
     2401 attgaagttg taacttgtag caaagtttaa attgttaaga agcattattt ttttaggttc
     2461 taccttggtg ctatctggat cagtaatttt agcttcgaaa gtattgctta aatcaaatcc
     2521 tactatatta gaatttgaat ttccgggtgc tccaaaaacg cctccttcaa atcgggtgta
     2581 atcttttgtc atttttcctg tagcatcggt agcataagta tcataatatt tttcgaaact
     2641 aggagtgtaa ccatacgaaa ttgaaggtct catcacgtgt ctgatggatt ttattttact
     2701 gtctggcttg aaattaaatg ttccgtaaat ggtggtacct aaactacttg taaaattata
     2761 ggttctaaaa gaatcaaatc cgttaacggt ttgatctaca acagcacttt gttctttgtc
     2821 atagtttctt cgaatggttt ttatggtcca aacttcatta taattcatag aagtggaagc
     2881 gctaaaatgt ttaaataatt tgaaattggt gcttaatgga atgctgtgtt gcactcctaa
     2941 ttttgcatta tcaaacattt cttttgtaaa aaacaaggag tcagttgtga caaaactgtt
     3001 tttaccactc aaattgtaca ataaattgat gtttttgaag aagccttttt ttaccccatc
     3061 tttacctaca aacggataaa ttcgatccac actatattgc aaatcaggca aggtcatatt
     3121 gatttcagaa gtttgagtgt tttgtgaatg tgttgcagtt agagctaaac gagaaccaac
     3181 tgtgcctgga aaagttttgc tataagaaac cgatgaactc aaagtattat tcaaattcga
     3241 ccctacattg gattggttta tagattgttg aaagtattta ctacttccca tatttacaga
     3301 agccgaaaaa gttgaattgg gactcccttt agagtcttta gaatgcgacc attgcagatt
     3361 ataaatactt tgtttggaat aatctggata gcctctttcg ctattgatta ggttttcaaa
     3421 tctaaagttt aaatttcctc taaaactgta tctttttgca taacttgatt cgaatctcag
     3481 ggcataactc ccgttggtat aataatcacc aagaacagtt aggtcataat tatcactaag
     3541 tgcaaaataa tatcctccat tttgcaatga aaatccccta ttatttgaat cgttataact
     3601 tggtaaaagg atacctgaaa cgctagtttc tttggtcatc ggaaaaaacg caaaaggcaa
     3661 agcgatagga gtgggcacat tggcaataac caaattagtt aagccggtaa ctactttttt
     3721 gccaggaatg atttttactt tattggtttg aaaataatat tcaggatttt ctacattagt
     3781 tgaagtagta aaacgtgctt tttttaagaa atagaccgaa tcattttctt tttttgtaat
     3841 tactgcttta accttgaatt ctccttgatc ggtttttgaa ttccatatca aagctttttt
     3901 tgttttgaaa ttaaaacg
//
LOCUS       DAOSNN010000242         3912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_23301_length_3912_cov_5.397718, whole genome shotgun sequence.
ACCESSION   DAOSNN010000242 DAOSNN010000000
VERSION     DAOSNN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23301_length_3912_cov_5.397718"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1589
                     /locus_tag="PLQ70_10650"
     CDS             48..1589
                     /locus_tag="PLQ70_10650"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HQE35117.1"
                     /translation="MAKQEDLFKNVVSHAKEYGFIFPSSEIYDGLSAVYDYAQNGVEL
                     KKNIREYWWKAMVQMNDNIVGLDAAILMHPTTWKASGHVDAFNDPLIDNKDSKRRYRA
                     DVLIEDYAEKLNLKAKKEIEKAKVRFGDAFNEQEFITTNARVVEYLAKEREILERMGR
                     SLGNGDLEDVKALIEELEIACPESGSRNWTEVRQFNLMFGTKLGASADTAMDLYLRPE
                     TAQGIFVNFLNVQKSGRMKVPFGIAQTGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GEEMQWYEHWKTARLNWHLSLGLGKENYRFHDHEKLAHYANAAADIEFNFPFGFKELE
                     GIHSRTDFDLKAHEQYSGRKLQYFDAELNQNYVPYVVETSVGLDRMFLAIFSTSLKEE
                     TLEDGSTRTVLKLPAVLAPTKAAILPLVKKDGLPEIANKIIDDLRWDFNVAYDEKDAV
                     GRRYRRQDALGTPFCITVDHQTIEDQTVTIRHRDTMKQDRVAIADLKSIIENEVSMKN
                     WLMKM"
     gene            complement(1661..2191)
                     /locus_tag="PLQ70_10655"
     CDS             complement(1661..2191)
                     /locus_tag="PLQ70_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HQE35118.1"
                     /translation="MKKLFALICLSVFAFGYSQEKEKEKIDFPRNEIKGNALFLVIGA
                     LEGTYERLLNEESGVGVSLFVPYDNSIDVNFSLTPYYRFYFGKKPAAGFFVEGFGMLN
                     SYDDTIYLTSTNSYIEKDVTRTDFALGFGLGAKWMTKNGFLFEINTGVGRNLFNSSDS
                     DFEIVGRGGITLGYRF"
     gene            2515..3597
                     /locus_tag="PLQ70_10660"
     CDS             2515..3597
                     /locus_tag="PLQ70_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQE35119.1"
                     /translation="MNYPYIIIDDNQESVLKTKAIADGFSELVFVASANNYADGLNLI
                     LEHSPKLIFLEIDPADTASNLSLALINELYRYLKVIPKIIITTSKKDLAFEAIQYEVA
                     DYIIKPVQKIDLIKSILKIKKSIGEDDGFIAQNQVAHQETKVEQPKIPVDVQKLIEEE
                     ILILNENDQELLEEHLKTPVDVQETLEEEILILNENDQELLDDQLQTTSDDTTEDDVV
                     ALDETQELLEGQIPEIYIQQSKPNVEQPLVLCIKSYGDYRYIDASDICYFQADNNSTD
                     IHLNNGEMVTAFKTLKHFEGVLPYPFARIHNSYIVNRNYISRIHTGNAVCYIKNTTTK
                     LPFSKSYKENVDLIISEFANGNYLEI"
BASE COUNT         1397 a          634 c          659 g         1222 t
ORIGIN      
        1 aaaaatgttt ctttttttaa gttcactttt tatatttttg caccactatg gcaaaacaag
       61 aagatttatt taagaatgta gtttcgcacg caaaagaata cggatttatt tttccgtcaa
      121 gcgaaatata cgatggtttg agtgcagttt atgattatgc acaaaacgga gtagagttaa
      181 aaaagaacat acgcgaatat tggtggaaag caatggttca aatgaacgat aacattgttg
      241 gtttggatgc agcgattttg atgcacccaa ccacttggaa agcatcgggt cacgtggatg
      301 ccttcaacga tccattgata gacaacaaag attccaaaag gagataccgt gccgatgttt
      361 tgattgaaga ttatgccgaa aagctaaatc taaaagcaaa aaaagaaatt gaaaaagcga
      421 aagttcgttt tggagacgct ttcaatgaac aagaattcat cacaaccaac gcaagagtgg
      481 tcgaatattt ggctaaagaa agagaaattc tagaaagaat gggacgttct ttgggtaatg
      541 gcgatttaga agacgtaaaa gcactaatcg aagaattgga aattgcttgt ccggaatcag
      601 gttctagaaa ttggaccgaa gtgcgtcaat tcaacttgat gtttggtacc aaattaggag
      661 catctgctga tacggcaatg gatttgtatt tgcgtccaga aaccgctcaa ggtatttttg
      721 tcaacttctt gaacgttcaa aaatcaggaa gaatgaaagt tccttttggt attgcacaaa
      781 ctggaaaagc ctttagaaat gaaatcgtag ccagacaatt tatcttccgt atgcgggaat
      841 tcgaacaaat ggaaatgcaa ttttttgtgc gtcctggaga agaaatgcaa tggtacgaac
      901 attggaaaac cgctcgtttg aactggcatt tatctcttgg tttaggaaaa gagaactacc
      961 gtttccatga tcacgaaaaa ttagcgcact atgctaatgc ggctgccgat attgagttta
     1021 atttcccttt tggattcaaa gaattagaag gaattcactc tcgtaccgat tttgacttaa
     1081 aagcccacga acaatattca ggtagaaaat tacagtattt tgatgccgaa ttgaaccaaa
     1141 attatgttcc ttatgtagtg gaaacttctg taggattgga tagaatgttc ttggcaattt
     1201 tctcaacttc attaaaagaa gaaactttgg aagacggttc aaccagaaca gttttgaagc
     1261 ttccagcggt tttagcacca acaaaagcgg ctattttacc tttggttaaa aaagatggat
     1321 taccagaaat tgccaataaa atcatcgacg atttgcgttg ggatttcaat gttgcttatg
     1381 acgaaaaaga tgctgtaggt cgtcgttaca gaaggcaaga tgctttagga actcctttct
     1441 gtatcacggt agatcatcaa actatcgaag accaaacagt aaccattcgt catagagata
     1501 caatgaaaca agatcgtgtc gctattgccg atttgaaatc aatcattgaa aatgaagttt
     1561 cgatgaaaaa ttggttgatg aaaatgtaat tacacattta tttataaaaa gaaaggcttc
     1621 tcattgagaa gcctttcttt ttatactatt tttataaatt ttaaaaccta taaccaagag
     1681 taattccgcc tcgaccaaca atttcaaaat cagaatcact actattgaat aaatttcgac
     1741 caacaccagt atttatttca aaaagaaatc catttttggt catccatttt gcgcctaatc
     1801 caaaacctaa tgcaaaatca gttctagtta catccttttc aatataagaa ttagtactgg
     1861 ttaaataaat agtatcatca taagaattta acattccaaa tccttctaca aagaatcctg
     1921 ctgctggttt tttaccgaaa taaaaacgat aataaggtgt aagactaaaa ttcacatcta
     1981 tgctgttgtc gtagggtaca aaaagggaaa ctccaactcc agattcctca ttaagtaatc
     2041 gttcgtatgt gccttctaat gcacctataa ccaaaaatag ggcgtttcct ttaatttcat
     2101 ttcttggaaa atcaattttt tctttttctt tctcttggct gtagccaaaa gcaaatacag
     2161 ataaacaaat tagtgcaaat aattttttca tagttttatt tttatttcaa atgtataaaa
     2221 tttttctgat tattgatgcg ctgttttata tatttagaat gagaataaag tattaatata
     2281 ttgcatttta tcggatttat ttgcagttaa ttttaatgta ttaacatttg ttactaacat
     2341 attaacaact taaatgtaaa atgatgtaaa tgtttacttt tagcttgtta aatagagtct
     2401 ttttatatga ttttatttaa ttagtttccc caaaactacc aaaattgctt aatattttaa
     2461 agtatttact ttaaacgtct aatgttatgc ctaacaaatt aggacaaaac attgatgaat
     2521 tacccgtaca ttattattga cgacaatcaa gaaagtgttt tgaaaaccaa agcaattgct
     2581 gacggttttt cggagcttgt ctttgtagct tcggcaaata actatgcgga tggtttaaat
     2641 ttaattctag agcattctcc caaattaatt tttctggaaa ttgacccagc tgatacagca
     2701 agcaatttgt cattggctct tatcaatgaa ttgtatcggt atttgaaggt aattcctaaa
     2761 attattataa ctaccagcaa gaaagatttg gcatttgaag ccatacaata tgaagtggca
     2821 gattatataa ttaagccagt tcagaaaatt gatttaataa aatcgattct taaaataaaa
     2881 aaatcaattg gagaggatga tggttttatt gctcaaaatc aagtagccca tcaggagact
     2941 aaagtagaac aaccaaaaat tcctgttgat gttcaaaagc tcatagaaga agaaattcta
     3001 attctaaatg aaaacgatca agagctttta gaagaacacc taaaaactcc tgttgatgtt
     3061 caagagacat tagaagagga aattctaatt ctaaatgaaa acgatcaaga gcttttagac
     3121 gaccaattac aaactacttc tgatgataca acagaagatg atgttgtagc tctcgatgag
     3181 acacaagagc ttttagaggg acaaatccca gaaatctata tacaacaatc aaaaccaaat
     3241 gttgaacaac ctctagtatt atgtattaaa tcttatggtg attatcgata cattgacgcc
     3301 agtgatattt gctattttca agccgataat aattcaacag atatccattt aaataatggt
     3361 gaaatggtta ccgctttcaa aacattaaaa cattttgaag gagttttgcc ttatcctttt
     3421 gctagaattc acaacagtta tattgtaaat cgtaattata tttccagaat tcatactgga
     3481 aatgcggttt gttatattaa aaacactacc acaaaacttc cattttcaaa atcttataag
     3541 gaaaatgttg atttaattat ctccgaattt gccaatggca attatttgga aatttaaaaa
     3601 aatccatcca cccagatttg atggtcattc actatcaaac gagtgcattc taccaaaacc
     3661 cctccatttt actgcaaaat tcaagataac cgatgtagtt ttacaccgtg atacttcact
     3721 atgaaggtca ccaaaaatgt aaaaacgaat taaaaacata aaaaacccaa actatgaaaa
     3781 agtcattctt aaccatcgga ttgttaacat tagtaatgat attaacttct ttcactacac
     3841 ctgaaactaa aattgatgct tcaggacaag gagcagcagg aggaaatgtt agattagaag
     3901 cttcaggaca ag
//
LOCUS       DAOSNN010000243         3885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_23498_length_3885_cov_4.171279, whole genome shotgun sequence.
ACCESSION   DAOSNN010000243 DAOSNN010000000
VERSION     DAOSNN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23498_length_3885_cov_4.171279"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLQ70_10665"
     CDS             complement(<1..627)
                     /locus_tag="PLQ70_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE35120.1"
                     /translation="MKISVVIAQEEHYKYAQEICDTIESSALLRGTGIAKRTPEYIKK
                     KITSGDAVIALDKNGVFAGFCYIESWQHGQYVAHSGLIVHPDFRNHGLAKKIKTFVFN
                     YSLKKYPGAKVFGITTGLAVMKINSDLGYKPVPFSELTSDPSFWKGCQTCTNYEILKS
                     KDNKMCLCTGMLYDPAEKPKDPPKHPFNVRIWNRLKEIKAALFLENKEK"
     gene            complement(1063..2244)
                     /locus_tag="PLQ70_10670"
     CDS             complement(1063..2244)
                     /locus_tag="PLQ70_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35121.1"
                     /translation="MKKHYFIFAFLLLTACGVKQTQNLLSSGNYDEAINNSISNLRSN
                     KDKKGNQDYVYLLEEAFAKAKERDLNAINLWEKEKNPNNFEKIYNTYIQLNNRQEKIK
                     PLLPLRLLKEGRNAIFPFDNYNDAMVSSKNNLSGYLYANAKSLLSTSDKMNFRKAFDD
                     LSYLNEINPNYKNVVSLMDEAHYKGTDYVFVNAKNETDKIIPIRLQNDLLDFNTYGLN
                     NKWTVFHGSKEKGINYDYGMTIRFQNILVSPEQIKEKEFIKERQIKDGQKRLLDANGK
                     EVIDNTGKVVMVDNMIKVTARIFESRQFKSSQIQAKVDYIDFRSNQLIQSFPLVSEFV
                     FENIYATCKGDKRASDDNYYSYFDKRAVPFPSNEQMVYDTGENLKTKLKELINRNTFR
                     N"
     gene            complement(2373..3656)
                     /gene="fahA"
                     /locus_tag="PLQ70_10675"
     CDS             complement(2373..3656)
                     /gene="fahA"
                     /locus_tag="PLQ70_10675"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HQE35122.1"
                     /translation="MPISANNTNRKSWIEVPADSDFPIQNIPFGVFITKDDVITIGTR
                     IGDCAIDMGALQQLNYFEGIELTDDMFMQDTLNDFISDGKKTWRLVRNRLAELFDETN
                     PKLRDNKEHREIVIFDVKDIEMQLPVLIGDYTDFYTSKEHAINIGKIINNPEKALPLN
                     WFHMPVGYHGRSSSIIPSGIPVHRPMVQSLPNGETTPVFGPSRMVDFELETAFITTDA
                     NIMGENIPINEAEDYIFGMVLMNDWSARDIQKWESLSFGPFLAKNFATSISAWIVTMD
                     ALEPFRIKGPNQDPAPFPYLLLKGKHNFDINLEVSIQTEKAINTIVSKTNFKYIYWSM
                     IQQLTHHTSNGCRVNSGDLMGSGTISGPTPDSFGSMLELTWGGKTPILLNDGTERKFI
                     SDGDTVIIKGFCKNNGARIGFGEVSTKLLPPFLRK"
     gene            3765..>3885
                     /locus_tag="PLQ70_10680"
     CDS             3765..>3885
                     /locus_tag="PLQ70_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35123.1"
                     /translation="MQRDKQIFDLILEEQDRQIHGLELIASENFVSDEVMEAAG"
BASE COUNT         1144 a          655 c          706 g         1380 t
ORIGIN      
        1 tttttctttg ttttccaaga acagggctgc tttaatttct ttcaatcgat tccaaattct
       61 cacattgaaa ggatgtttgg gcggatcttt cggtttttcg gccgggtcgt aaagcattcc
      121 tgtacacaaa cacattttgt tgtctttgct tttcagaatc tcgtaattgg tacaggtttg
      181 gcagcctttc cagaaacttg gatctgaagt aagttccgaa aaaggaacag gtttataccc
      241 taaatcggaa ttgattttca ttacagccaa accagtcgtg ataccaaaaa ctttggcacc
      301 tggatatttt ttcaaggaat aattgaaaac aaaggtcttt attttcttgg ccaaaccgtg
      361 atttctaaaa tcaggatgaa caatcaatcc agagtgcgcc acatactgac cgtgttgcca
      421 actttcgata tagcaaaaac cagcaaaaac gccattttta tccaaggcaa taacggcatc
      481 tccgctagta atcttttttt tgatgtattc tggggttctt tttgcaattc cagtacctct
      541 taataaagcc gaagattcta tagtgtcgca tatttcttgc gcatatttat aatgttcttc
      601 ttgagctata actacagata ttttcatttc aagagttaaa gtttgggtta aaaaataaaa
      661 attttgttgg tttcgaaaag ctgttcttgt tcgagtttat cgttgcatta ctaccataaa
      721 aaatcgcccc taaaagcaga gtgcaggata ataagcagaa aagaatggta ttttcagaat
      781 gaaataatcc gatggatgtt attaataatt tcaaaatgat aaaaaatgaa aaatgaataa
      841 aaaaacacat ttggagctat attacagcat ccgtacttcg ggcgaagcgg ttggtttgtt
      901 tatccgtgat aaagtagtta tatgtaaact tgtatttttc attggtgcaa aattacgatt
      961 tatttattaa ttcactcctt tttttttcta acaaaattga aattaattgt taattttcaa
     1021 taaataaact gtttgggatg aaatagcttg aatagtaatg ggttagtttc taaaagtatt
     1081 tctgttaata agctctttta attttgtttt caagttttcg cctgtgtcat acaccatttg
     1141 ctcgttggat ggaaaaggaa cggctctttt gtcgaaataa gaatagtaat tatcgtccga
     1201 agcgcgtttg tcgcctttgc aagtggcata aatattttca aaaacaaact cactaactag
     1261 tggaaacgat tgtattaatt gattgcttcg aaaatctata taatctactt ttgcctgaat
     1321 ctggctggac ttgaattgcc tagattcgaa tattcgtgcc gtcaccttta tcatattgtc
     1381 taccatgacc acttttccgg tattgtctat tacttctttt ccattggcgt ccaacagtct
     1441 tttttgaccg tcttttattt gtctttcctt gataaattct ttttccttga tttgttctgg
     1501 cgaaaccaat atgttctgga aacgaatagt cattccgtaa tcataattga ttcctttttc
     1561 tttgctgccg tgaaatacgg tccatttgtt gtttaatcca taggtgttga agtccaataa
     1621 atcattttgc aaacggatag gaataatttt gtcggtttcg tttttggcat tgacaaatac
     1681 gtaatcggtt cctttgtaat gtgcttcgtc catcaaacta accacgtttt tgtagttggg
     1741 attgatttcg ttcaggtaac ttaaatcgtc gaatgctttg cggaaattca ttttgtccga
     1801 agtggacaac aaggatttgg cattggcata cagataaccg gacaagttgt ttttgctgga
     1861 caccattgcg tcattgtaat tatcgaaagg gaaaatggcg tttctgcctt cttttagtaa
     1921 tctcaaaggt agcaatggtt ttattttttc ttgtcggttg ttcagttgaa tataagtatt
     1981 gtatattttt tcgaaattgt tcggattttt ttctttttcc caaagattga tggcattcaa
     2041 atcgcgttct ttggctttgg cgaaagcctc ttccagcaaa taaacgtaat cttggtttcc
     2101 ttttttgtct ttgttggaac gtaaattcga tatggaattg ttgatggctt cgtcataatt
     2161 cccggaactc aacaagtttt gggtttgttt gaccccacag gcagttagta gtagaaaagc
     2221 gaatataaag tagtgttttt tcatctttaa atttttttgc gaatttacaa taattttgaa
     2281 ctgtattttg agttttttgt aggctaaaat aatgccaaaa aaaatccgac actttaaata
     2341 aatgtcggat tatatataag gtaaaaaagt ttttattttc tcaagaaagg aggtagtaat
     2401 ttagttgaaa cttcgccaaa accaattcgg gcaccattgt ttttgcaaaa tccttttatg
     2461 attacagtat caccatcgct gatgaatttt cgttcggttc catcgttgag gagtattggg
     2521 gtttttcctc cccaagtcaa ttctagcatt gagccaaaac tatcgggagt tggtcctgaa
     2581 atagttcctg atcccatcaa atcacctgaa tttactcggc aaccattaga ggtgtgatgg
     2641 gttaattgtt gtatcatcga ccaatagatg tatttgaaat tggttttaga aacaatggta
     2701 tttattgctt tttccgtttg gatggaaact tctaaattga tatcaaaatt gtgttttcct
     2761 tttaaaagta aataaggaaa cggagcaggg tcttggttag gaccttttat tcggaatggt
     2821 tctaaggcat ccattgttac tatccaagct gagatggaag tagcgaaatt tttagccaag
     2881 aaaggaccaa atgataatga ttcccatttt tgaatgtcac gagcactcca atcgttcatt
     2941 aaaaccatgc caaaaatata atcttcggct tcgttaatgg gaatgttttc tcccataata
     3001 ttagcatcgg tagtaatgaa agcggtttct aattcaaaat cgaccattct tgatggccca
     3061 aaaacaggcg tggtttcgcc atttggtagc gattgaacca ttggtctgtg aactgggatg
     3121 ccagaaggaa ttatggagga acttcttccg tggtagccaa caggcatgtg aaaccaattc
     3181 agtggtaagg ctttttcggg attattaatt attttaccta tattgatagc atgttctttg
     3241 ctagtgtaaa aatcagtata atcgccaatt aaaacgggca attgcatttc gatgtccttt
     3301 acatcaaaaa ttacaatttc tctatgttct ttattatctc taagttttgg attggtttcg
     3361 tcaaacaatt cagccaagcg atttcgaacc aatctccaag ttttttttcc gtccgaaata
     3421 aaatcattta aggtgtcttg catgaacata tcatcggtca attcgattcc ttcaaaataa
     3481 tttaattgct gtaaagcccc catatctatg gcacaatcgc ctattcgagt gccaattgta
     3541 atcacgtcgt cttttgtaat aaaaacccca aatggaatgt tttgaatagg gaaatcactg
     3601 tcggcaggaa cttctatcca agattttctg tttgtattat tagctgatat tggcatacga
     3661 ttgttaatta tattgttaag aattactcat caaatatatc ataatcttac tatttaccaa
     3721 acgttttttt gtatttttgc gccaaattaa ctaaaaacaa agaaatgcaa cgcgacaaac
     3781 aaatttttga ccttatccta gaggaacaag acagacaaat tcatggatta gaacttatcg
     3841 cttcagagaa ttttgtaagt gatgaagtaa tggaagcagc tggtt
//
LOCUS       DAOSNN010000244         3869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_23621_length_3869_cov_4.804929, whole genome shotgun sequence.
ACCESSION   DAOSNN010000244 DAOSNN010000000
VERSION     DAOSNN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23621_length_3869_cov_4.804929"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1295
                     /locus_tag="PLQ70_10685"
     CDS             102..1295
                     /locus_tag="PLQ70_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQE35124.1"
                     /translation="MNNDFIKYQAQTSPYPLGMEVSHAKGSYIYDTNNKKYLDFTAGV
                     SACTLGHQPPRVNQAIKDQLDKYSHVMVYGEYSQSPAVEYCKLMASLLPKPLDKTYLV
                     NSGTEAIEGALKLARRTTGRSQLISCHNAYHGNTMGSMSVMGFEERKQIFRPLIPDVD
                     FITFNNEADLEKITTKTAGILLETIQGGAGFIEPHNNFLKKVRERCTEVGAMMILDEI
                     QPGFGRTGKLFGFQNYDVVPDIVVMGKGMGGGMPVGAFTASSEMMDLLSHDPKLGHIT
                     TFGGHPVIAAASLATLQEITETNLMAETLEKEKLFKSLLVHPLIKEIRGKGLMLAAMT
                     ETAEITNEVILKCQDKGLILFWLLFEGCAIRITPPLTISEDEIREGCAIMIEVLNEVQ
                     LTINN"
     gene            1410..2804
                     /locus_tag="PLQ70_10690"
     CDS             1410..2804
                     /locus_tag="PLQ70_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE35125.1"
                     /translation="MQLSDEEEDYNLSLSKFESMLKTNKVLFFDSEEFEDIILHYLDM
                     GKANLAKKALKLALEQHPRSTGLKLVQVEMLVYDDKLDQAEKLLNELYAIEPTNEEIY
                     IQQANIFSKRDNHEKAVELLNIALKYTDDYADVYNLMGMEYLFMDNLEMAKESFIKCL
                     EEDFEDQSALYNVVYCFEFLDQNQEAIDYLTQYIDKNPYSEIAWHQIGRLHYGLKEYE
                     NAIRAFDYATLIDDEFLGAFMERAKALERLKKYEEAIESYNRTIELDDATSYALLRIG
                     KCYEKLGNKVQALNFFNKTVHEDPLLDKGWIAITDFYVRQKNFQKALFFVNKALAIDN
                     QNRMYWKRYATINKQMNFFEEAEFGYRKAVEFGDYALDTWLFWVDILQFLGEFETCIQ
                     TLLQASEYFPEENEVEYRLAGLYFMIQDTTKAKFHLSNALRLNFDNYIILEDLFPVVW
                     TRKMVQNYIAKHKK"
     gene            2955..3707
                     /locus_tag="PLQ70_10695"
     CDS             2955..3707
                     /locus_tag="PLQ70_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQE35126.1"
                     /translation="MLDTIRKRFGLLGRNISYSFSKGYFTEKFSKDELFEGCTYENFD
                     IPEITAFPEIIKNTPDLKGMNVTIPYKEVVIPYLDKLSKKATEIGAVNTIKFTKKGKL
                     KGYNTDYYGFMKSLEPLLQPHHKKALILGTGGASKGVAYALKELGILYTFVSREAKEG
                     IIDYDRINATTFDNYQIIINSSPVGTSPNIDAFPLLPYEYFTQKHIAYDLIYNPAETQ
                     FLKKAAAQGAQTKNGQDMLIFQAEEAWRIWNK"
BASE COUNT         1314 a          720 c          737 g         1098 t
ORIGIN      
        1 cattccgacg aaggaggaat cccaacaaac gagagctact actataatta cttcttctca
       61 gaagaatgag ttataagata aaaaaaatca acacataaaa cgtgaacaac gatttcataa
      121 aataccaagc ccaaacgtct ccctatcctt taggaatgga ggtttcgcac gccaaaggct
      181 cttatattta cgacaccaac aataaaaaat atttggattt tacagcagga gtttcggctt
      241 gcacactcgg acaccaaccg ccaagagtca atcaggcgat aaaagatcaa ctggacaaat
      301 attcgcacgt gatggtgtat ggcgaatatt cgcaaagtcc agctgtggaa tattgcaaat
      361 tgatggcttc actcctaccc aaacccttgg acaaaaccta tttggtcaat tccggtacag
      421 aagccattga aggcgccttg aaactcgccc gaagaaccac cggcagaagc caattgattt
      481 cttgccacaa cgcctatcac ggcaacacga tgggttcgat gagcgtgatg ggattcgaag
      541 aacgcaaaca aatttttcgt ccgttaattc ccgatgtcga ttttatcact ttcaacaacg
      601 aagccgattt agaaaaaata accaccaaaa ctgccggaat cctactcgaa acgattcaag
      661 gtggtgccgg ttttatagaa cctcataata atttcctgaa aaaagtccgc gaacgttgca
      721 ccgaagtagg cgcaatgatg attctcgacg aaatccaacc gggtttcggc agaacaggta
      781 aactttttgg tttccaaaat tacgatgtcg ttcctgatat tgtagtgatg ggaaaaggaa
      841 tgggtggtgg aatgcctgtg ggagctttca cggcttcctc agaaatgatg gatttgttga
      901 gccacgatcc caaactaggt catatcacga cttttggagg ccatcctgtc attgcggcag
      961 ctagtttggc cactttgcag gaaattacag agacaaacct gatggcagaa actttagaaa
     1021 aagaaaagct cttcaaatcg cttttggtac atcctttgat aaaagaaatt agaggaaaag
     1081 gattgatgtt ggcagcaatg acggaaactg ccgagattac caacgaagta attctgaaat
     1141 gccaagacaa aggattgatt ctattttggc ttttatttga aggatgcgcc attcgaatta
     1201 cacctccttt aaccatttca gaagatgaaa ttagagaagg ttgtgctatt atgatcgaag
     1261 tcttgaacga agttcaattg acaattaata attgataatt atcaattcat tttctgttga
     1321 ttaaattgtt cacaacaatt ccaaattttc ctcacatcaa tacctaggtt gcctactttt
     1381 attttgggca aactcaaaaa aaattaatta tgcaattaag cgacgaggaa gaagactata
     1441 acttatccct atccaaattt gagtcgatgt tgaaaaccaa caaagtgctc ttttttgact
     1501 ccgaggaatt cgaggatatc attcttcatt atctcgatat gggaaaagcc aatttggcca
     1561 aaaaagcttt aaaattagct ttggaacaac accctagatc aactggacta aaattagttc
     1621 aggttgaaat gttggtgtat gacgacaagc tcgatcaggc tgaaaagcta ttgaacgaat
     1681 tgtatgccat agaaccaacc aatgaagaaa tttacattca acaagccaat attttttcta
     1741 aaagagacaa ccacgaaaag gcggttgaat tactaaatat tgctttgaaa tacaccgatg
     1801 attatgccga tgtctataat ttaatgggga tggaatacct gtttatggac aatctcgaaa
     1861 tggccaaaga aagctttatc aaatgtttgg aagaagattt cgaagatcaa tctgccttat
     1921 ataatgttgt ctattgtttt gaatttctag atcagaatca ggaagccatt gattatttaa
     1981 cccaatacat tgacaaaaat ccgtatagcg aaatcgcttg gcatcaaatc ggtcgtttgc
     2041 attatggatt gaaagaatac gaaaatgcca ttcgtgcttt tgattatgcg acacttatcg
     2101 acgacgaatt tttgggtgct tttatggaaa gagccaaagc tttggaacgc ctgaaaaaat
     2161 acgaagaagc catcgaaagc tacaacagaa ctatcgagtt agacgatgct acatcttatg
     2221 ctttgcttcg aattggaaaa tgttacgaaa aattaggcaa caaagttcaa gccttaaact
     2281 ttttcaacaa aacggttcac gaagatcctc ttttggacaa aggttggatt gctattaccg
     2341 atttttatgt gcgtcaaaag aactttcaaa aagccctatt ttttgtcaac aaagcattag
     2401 cgattgacaa tcaaaacaga atgtattgga aacgctatgc tacgataaac aaacaaatga
     2461 acttctttga ggaggccgaa tttggataca gaaaagcggt agaatttggc gattatgcct
     2521 tagatacttg gctgttttgg gtagatattt tacagttttt gggcgaattc gaaacttgta
     2581 ttcaaacttt attacaagcc tcagaatatt ttccagaaga aaacgaagtc gaatatcgtt
     2641 tggcgggatt gtattttatg attcaggata caaccaaagc caagtttcat ttgagcaacg
     2701 ctttgcgatt gaattttgac aattacatca ttttggaaga tttatttccg gtggtttgga
     2761 ctcgaaaaat ggtgcaaaac tacattgcga aacacaaaaa ataagtaatt tatttgaccg
     2821 caaatttagc tcataggcga tgatctattc ggtattcgct gatttttcaa ttcacaaatt
     2881 ttcacaaatt aatttgcggt aatttgtgaa atttgtggtt ttaaaaaacc tttaaaaact
     2941 caatataaaa tataatgcta gacacaatac gcaaacgctt tggtttactg ggacgcaaca
     3001 ttagttattc tttttcgaaa ggctatttta cagaaaaatt cagcaaagac gaacttttcg
     3061 aaggttgtac ttacgagaat tttgacattc ctgaaataac cgcttttcct gaaatcatta
     3121 aaaacacgcc cgacctaaaa gggatgaatg taacgattcc gtacaaagaa gtcgtgattc
     3181 cgtatttgga caaattgtct aaaaaagcaa ccgaaattgg agctgttaac acaataaaat
     3241 ttaccaaaaa aggtaaattg aaaggataca acacggatta ttatggtttt atgaaatcat
     3301 tagaaccgct attgcaaccg catcacaaaa aagccttgat tttgggaact ggtggcgctt
     3361 ctaaaggtgt ggcttatgct ttgaaagaat tgggaatact atacactttt gtttcccgcg
     3421 aagccaaaga aggcattata gattatgacc gcatcaacgc tactactttt gataattacc
     3481 aaatcatcat caattcgtca cccgttggaa caagcccaaa tattgatgct tttccgttac
     3541 ttccttacga atatttcacc caaaaacaca ttgcttacga cttgatttac aatcctgccg
     3601 aaacccaatt tctaaaaaaa gcagcagcgc aaggagcaca aaccaaaaac ggacaggata
     3661 tgcttatttt tcaggcagag gaagcttgga ggatttggaa taagtaggga ttacatttaa
     3721 tcatttgtaa gaaaaaatta atatctttga ttacaaataa agtctgattg ccgtcagact
     3781 tatccaccca aaattgggtt cataggtctg attttatcag acctatatac tagttggacg
     3841 caatggccga gccaatgcgt ccaataagc
//
LOCUS       DAOSNN010000245         3866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_23644_length_3866_cov_3.896090, whole genome shotgun sequence.
ACCESSION   DAOSNN010000245 DAOSNN010000000
VERSION     DAOSNN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23644_length_3866_cov_3.896090"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..1202)
                     /locus_tag="PLQ70_10700"
     CDS             complement(399..1202)
                     /locus_tag="PLQ70_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQE35127.1"
                     /translation="MSQDTKELKLAVLIDADNVPYSNVKGMMEEIAKYGTPTTKRIYA
                     DWTKPNANGWKSVLLEHAITPIQQYSYTVGKNSSDSALIIDAMDLLYSDKVDGFCIVS
                     SDSDFTRLAIRLRESGMKVIGIGEKKTPNSFIVACDRFIYIEVLDGAIKKTRKTTNSQ
                     SKTPKPTPTPVAESKKPVEKETPNKIDNQTIELIEDTLDAIGDEDGWAFLGDVGNLIV
                     KKKPEFDPRNYGFSKLTPMLKSLTDILEIDERESDKKGIKHVYVRLRFA"
     gene            complement(1206..1790)
                     /locus_tag="PLQ70_10705"
     CDS             complement(1206..1790)
                     /locus_tag="PLQ70_10705"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HQE35128.1"
                     /translation="MKNKNILIILFLFVAMAQLFVPSQMIYNQEDILNTGKMLKFQCE
                     PIDPNDPFRGKYITLNFKEEGIKVKNLKEWNRNETIFAKIETAKDGFAKIKSISRTEP
                     TDNSIYLKLKIDYIADYDYEHKIYLDFPFDRFYMNENKAKTAETVYAESTIDTTKIAY
                     ALVATKNGEAVIKDVLIDNISIKELAKAKNNSNK"
     gene            complement(1780..3063)
                     /locus_tag="PLQ70_10710"
     CDS             complement(1780..3063)
                     /locus_tag="PLQ70_10710"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HQE35129.1"
                     /translation="MSNSILNELHALLKANVINEETAQKIKEHYTKNGDQSQSKLFMI
                     FGILGAILSGLGIILILAHNWDDLSNSIKSILSFLPLVIGQLFCGYTILKKAETPIWR
                     ESSSVFLFFGIGASISLIAQVYNISGDLNSFLITWMLLSLPLVYLLRTSIVSLLYIIG
                     ITYYCCNSNYFTYPKEQNYLFWLLFLGMIPHYYQLLTTKSQSNFTLFHNWFVAISLVI
                     SLGSIRDGNEKLLYLSYMCLFAIYYFIGKSSFFKNQKLKFNSYRIIGKLGTLILLSVY
                     TFKSFWNFFHEKKVHENPIITSPEFIITSILSIIAAVLFYKKNNTTHFKNFDLIEIIF
                     LLNIPLLFMGEVAAIVANGIVLAIGLLEIKRGNQLHNLRILNFGLLIITILITCRFFD
                     TDFSFIVRGILFISLGLGFFLTNYVMLKKRNRNEK"
     gene            3267..>3866
                     /locus_tag="PLQ70_10715"
     CDS             3267..>3866
                     /locus_tag="PLQ70_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQE35130.1"
                     /translation="MVVDLFARIQNNKGPLGKWASQAEGYFVFPKLEGDLGPRMKFQG
                     KEVLNWSLNDYLGLANHPEVRKADADAALEFGAAAPMGARMMSGHTKYHEQLENELAA
                     FVKKESAYLLNFGYQGMVSIIDALVTRNDVIVYDVDGHACIIDGVRLHSGKRFTYKHN
                     DIESMEKNLQRATKLATEQGGGILFITEGVFGMRGQQGKL"
BASE COUNT         1260 a          551 c          705 g         1350 t
ORIGIN      
        1 caatataata tttttgataa tttaaaataa tattttaacg caccagcact aatttgtttt
       61 tataaattta ttaaaagtaa tgtaactatt tggtagataa aaaaacttac cacagttttt
      121 ccaaattagg aatcaattta gttattaaaa gctgatgtat tttattgtaa aaaagaagaa
      181 ttccattttt tttacccagt tacattgtgt caaaacaatt ttattttggc attggaaggt
      241 ctgctgttgc aatttttttt attatatatt aaaaattaag tagtttaagt ttaataatgg
      301 tttggattag ttgcttattt atataaatat taaaataagg atgattattt aataattaac
      361 gaaaggatgg ttgagtaata ttattgtcta taatgaaatt atgcgaagcg caagcgcaca
      421 tacacgtgct tgatgccttt tttgtccgat tctctttcgt caatttcaag aatatcagtt
      481 agggatttca gcataggtgt tagtttagaa aatccataat ttctagggtc gaattctggt
      541 tttttcttta caattaagtt tccaacatct ccaagaaatg cccagccatc ttcatctcca
      601 atggcgtcga gggtgtcttc tatgagttct atagtttggt tgtcgatttt gtttggggtt
      661 tctttttcta caggtttttt agattctgct acaggagttg gagttggttt tggagttttg
      721 ctttgtgagt tggtcgtttt tctagtcttt ttgatggcgc cgtccaaaac ttctatgtaa
      781 ataaatctat cgcaagctac aatgaacgaa tttggtgttt ttttctcgcc aattccgata
      841 actttcatcc ctgattcacg aagtcggatt gctaatcgag taaaatcgct atcgctggaa
      901 acgatgcaaa aaccatctac tttgtcggag tacaacaagt ccattgcgtc aataatcaaa
      961 gcagaatctg aggaattttt cccaactgtg taactgtatt gctgaattgg agtgatggcg
     1021 tgttccaaca aaaccgactt ccagccgttg gcattgggtt tagtccaatc agcataaata
     1081 cgttttgtgg ttggagtgcc gtatttggcg atttcttcca tcattccctt tacgttgcta
     1141 tacgggacat tgtcggcatc aataaggacg gctagtttta attctttggt atcttgtgac
     1201 atattttatt tgttactatt atttttggct tttgccaatt ctttaatgga tatattatcg
     1261 attaaaacat cttttattac tgcttctccg ttttttgtag cgactaatgc gtaagctatt
     1321 tttgtggtgt caattgtaga ttcggcatat actgtttcgg cggtttttgc tttgttttca
     1381 ttcatataaa aacgatcaaa cggaaaatcc aaatatattt tgtgttcata atcatagtca
     1441 gcaatatagt cgatttttag ctttaaataa atcgaattat ctgtaggttc tgttcttgaa
     1501 atggatttaa ttttcgcaaa tccgtcttta gcagtttcaa ttttggcaaa gatagtttcg
     1561 tttctattcc attcttttaa atttttgacc tttattccct cttctttaaa gtttaaagta
     1621 atgtattttc ctctaaaagg atcgttgggg tcgataggtt cgcattgaaa ttttaacatt
     1681 ttgcccgtat ttagaatatc ttcttggttg taaatcattt gagaaggaac aaataattgt
     1741 gccattgcaa cgaaaaggaa tagtataatt aggatgtttt tatttttcat ttctatttct
     1801 tttttttagc attacataat ttgtcaagaa aaaaccgaga cccaacgaaa taaataggat
     1861 tcctcgaact ataaaactaa agtcggtatc aaaaaaacga caagtgatta atatagtaat
     1921 aatcagcaaa ccaaaattta gaattcttaa gttatggagt tgatttcctc gttttatttc
     1981 taaaaggcct attgccaaaa caattccatt ggcaacaatt gcggcaactt cacccataaa
     2041 tagtagagga atattcagca gaaaaataat ttctattaag tcaaaatttt tgaaatgggt
     2101 tgtattgttt tttttgtaaa ataaaacagc agctataatg gatagaattg aagtaattat
     2161 aaattcggga gatgtaataa tcggattttc gtgtactttt ttttcgtgga aaaagttcca
     2221 aaaacttttg aatgtataaa cggaaagtag gatgagggtt cctagtttac caataattcg
     2281 gtaactattg aatttgagtt tttgattctt gaaaaaggaa ctttttccaa tgaaatagta
     2341 aatggcaaac aaacacatat acgataaata caataatttt tcgtttccat ctctaataga
     2401 acctaggctt attactagag aaatagctac gaaccaattg tgaaataagg tgaaattgct
     2461 ttgtgatttt gtggttaaaa gttggtaata atgaggtatc atacccaaaa ataaaagcca
     2521 aaaaagatag ttttgctctt tgggataagt aaaataattt gaattgcaac aataataagt
     2581 aatgcctatg atgtataaaa gagatactat cgatgttcgt agcaaataaa ccaagggcaa
     2641 actcaggagc atccaagtga taagaaatga gtttaaatcg ccagaaatat tataaacttg
     2701 ggctattagc gatatgcttg ctccaatacc aaaaaataga aaaacagatg aactttctct
     2761 ccagattggg gtttcagcct ttttaagaat ggtgtaaccg caaaataatt gtccaattac
     2821 tagcggaaga aacgatagta ttgatttaat ggaattggat aaatcatccc aattatgagc
     2881 aagaataagt attattccca atccggatag gattgctcct aaaatcccaa aaatcataaa
     2941 taacttactc tggctttggt caccattttt agtgtaatgt tcctttattt tttgagctgt
     3001 ttcttcatta atgacatttg ctttgagtaa agcgtgaagt tcatttaaaa tggaattgga
     3061 cataacatag attttgttaa ccaaatatac aaaatttatt gttaaatctg ctaaatagtc
     3121 aggaggaaac ggcatccttt tacttttttc ttaaaaagta aaagatacag taaacagctg
     3181 gaaatagctc ctaaaaaaac attatgataa gacagattaa ataattaatt ttgcacttca
     3241 aaaaatatat aatacttcaa aatagtatgg tagtagattt attcgcaaga attcaaaaca
     3301 ataaaggacc attgggaaaa tgggcttcac aagcagaagg ttattttgta tttccaaaat
     3361 tagaaggaga tttaggacca agaatgaaat ttcaaggaaa agaggttttg aactggagtt
     3421 tgaatgatta tttaggtcta gcaaatcatc cagaagttcg taaagcagat gcggatgcgg
     3481 ctttagaatt tggtgctgct gcgccaatgg gagctcgtat gatgagcgga cacaccaaat
     3541 atcacgaaca attagaaaat gaattagctg ctttcgttaa gaaagaatcg gcttatttgt
     3601 tgaattttgg gtatcaagga atggtgtcta tcattgacgc tttggtgact agaaatgacg
     3661 tgattgtgta tgatgtagat ggtcacgctt gtattattga cggtgttcgt ttgcatagtg
     3721 gaaagcgttt tacgtacaaa cacaacgaca ttgaaagtat ggagaaaaac ttgcaacgtg
     3781 ctacaaaatt ggctacggaa caaggtggag gaattctttt tattaccgaa ggtgttttcg
     3841 ggatgcgtgg tcaacaagga aagttg
//
LOCUS       DAOSNN010000246         3856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_23723_length_3856_cov_3.676927, whole genome shotgun sequence.
ACCESSION   DAOSNN010000246 DAOSNN010000000
VERSION     DAOSNN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23723_length_3856_cov_3.676927"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..597)
                     /gene="cas2"
                     /locus_tag="PLQ70_10720"
     CDS             complement(268..597)
                     /gene="cas2"
                     /locus_tag="PLQ70_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HQE35131.1"
                     /translation="MELNAYRIMWLFVFFDLPTETKKDRRNASGFRNNLLKDGFSMMQ
                     YSVYVRHCASGESADVHEKRIHNLLPPLGKVSVLRITDKQFGNILNFWGKAEVPKAPQ
                     PTQLELF"
     gene            complement(573..1481)
                     /gene="cas1"
                     /locus_tag="PLQ70_10725"
     CDS             complement(573..1481)
                     /gene="cas1"
                     /locus_tag="PLQ70_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HQE35132.1"
                     /translation="MLKKSILIENKTAITAKNLQLVIKSEIRESAIPIEDIGFLVLDH
                     QEIYISLPAMNLLVENNTSVIICSKNHLPNGMFLNLNSHHIQQEVFKNQIDASIPLKK
                     QLWQQTIVEKITNQGLLLERITQNKNSFEFLASKVLSGDTSNMEGVAASQYWKSFFDT
                     YDIKFRRERFGDYPNNFLNYGYAILRAATARALSGSGLLNTLGIHHKSKYNSFALADD
                     IMEPFRPLVDEKVFEIMQTYDEQELNTKIKAELLQVLTRTVYFKEEKSPLMVALQKTA
                     SSLQQCFMGQRKKIKYPKLWNLTPTE"
     gene            1753..2883
                     /gene="tgt"
                     /locus_tag="PLQ70_10730"
     CDS             1753..2883
                     /gene="tgt"
                     /locus_tag="PLQ70_10730"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQE35133.1"
                     /translation="MKFDLLQKDPHTKARAGSITTDHGVIETPIFMPVGTVASVKGVH
                     QRELKEEINPDIILGNTYHLYLRPQTEILEKAGGLHKFMNWDRNILTDSGGYQVYSLS
                     SNRKIKEEGVKFKSHIDGSYHVFTPENVMEIQRTIGADIIMAFDECTPYPCDYRYAQR
                     SMHMTHRWLDRCINHLEKVPFKYGYEQTFFPIVQGSTYKDLRRQSAEYIANSGQQGNA
                     IGGLSVGEPAEEMYAMTEVVCEILPEDKPRYLMGVGTPINILENIALGIDMFDCVMPT
                     RNARNGMLFTANGTINIKNKKWEDDFSVIDEMGHTFVDTEYSKAYLRHLFAANEYLGK
                     QIATIHNLGFYMWLVREARKHILAGDFRTWKEMMVRNMSQRL"
     gene            2971..>3856
                     /locus_tag="PLQ70_10735"
     CDS             2971..>3856
                     /locus_tag="PLQ70_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQE35134.1"
                     /translation="MLTIIDKYILKKYLATFGVMLGLFAPIGIVIDISEKINKMIENK
                     IPIMEILVYYYHFTIYFMNLLFPIFLFLSVIWFTSKLANDTEIIAILSSGISFTRFLR
                     PFMIGATIVSLIVLLMGFFIVPKSNEGFNNFRYTYLRGDGKETVRGKNTDVFRQLSND
                     EYIYVNSFDVASKTAYNFTYEKFTKDKLAYKISASRIQWNPKTKNYTMYDYVKRTVGG
                     LDDKIEKSDKKDAKFSFDLDDITPVIYIAETLSLGKLNQFIEKEKKRGSSNINVYKVV
                     LYKKYSIPVSAFILTVIAV"
BASE COUNT         1201 a          610 c          778 g         1267 t
ORIGIN      
        1 cttggtgcag tggttgtggt ttgattaaag attagaaaac acgatattta tgacaaacac
       61 gttatcgcag attttgaagt tgtggtttga ttaaagatta gaaaacacga tattagttgg
      121 cttgttacct tctgatattc atagaattaa aagtaaatat cgtataaaaa aatgcttctt
      181 tattggtttg ataacccaat attgaggcat ttttttgttt ctagaaacca ccccgccctt
      241 tgggcacccc tccagtggag gggaagatca aaataattct aattgtgtgg gttgaggtgc
      301 tttggggact tcggctttgc cccaaaaatt taggatattg ccaaattgct tgtcggttat
      361 tcgtaaaaca ctcactttgc ccaatggagg caaaaggttg tgaatgcgtt tttcgtgtac
      421 atcggcactt tcgccactgg cacaatggcg aacataaacc gaatattgca tcatcgaaaa
      481 accatctttg agcaagttgt tccgaaatcc cgaagcgttt cttcggtctt ttttggtttc
      541 tgttggcaaa tcaaaaaaca caaacaacca cattattcgg taggcgttaa gttccataat
      601 ttgggatatt taattttctt tctttgtccc ataaagcatt gctgcaagga gctagccgtt
      661 ttttgcaagg ctaccatcaa cggacttttt tcttccttga aataaacggt tcgggttagt
      721 acttgcagga gttcggcttt gattttggtg tttaattcct gttcgtcata ggtttgcata
      781 atttcaaaaa ctttttcgtc aaccaagggg cggaacggtt ccataatatc atcggcaaga
      841 gcaaaggagt tgtatttgct tttgtgatga atgcccaagg tgttcaataa accgctgcca
      901 gaaagtgctc tggcggttgc tgctcgaaga atggcatagc cataattgag gaaattattg
      961 ggataatctc caaaacgttc ccttctgaat ttgatgtcgt aagtatcaaa aaaagatttc
     1021 cagtattggc ttgctgcaac gccttccata ttagaagtgt ctccgctcaa aactttgctg
     1081 gctaggaatt cgaaactgtt tttgttttgg gtgattcttt ctaaaagcaa accttgattg
     1141 gttatttttt cgacaatggt ttgttgccat aattgttttt ttagtggaat ggaagcatca
     1201 atttggtttt tgaagacttc ttgctggatg tggtgactgt tgaggttcag gaacattccg
     1261 ttgggcaaat gatttttaga acaaataatt acggatgtat tgttttctac caataaattc
     1321 atagctggta aactaatgta aatttcttga tggtctaaaa ccaaaaaacc aatatcttca
     1381 atgggaatag cactttctct aatttcagat ttaatgacta attggaggtt tttggcagta
     1441 atagccgttt tattttctat taaaatggat ttttttagca taatagtagg attagatatt
     1501 gtagaagact aatttactga tagatatgat ggtgttttta cgggaaacct cattttcata
     1561 attttttttg gtttgcgtga agtatggaag cgacatcctc gtagtgaagc ggagagatat
     1621 agcggacagc ctgacagcat catagcttga ggaggtgatg gcacgctcaa ataaatacat
     1681 tgttaaaaaa actcacaatt cacaattcac aattcacaat taacttatct ttgcgcactg
     1741 aaaaaagcac aaatgaaatt tgatttatta caaaaagatc cacataccaa agccagagca
     1801 ggaagtatta ctactgatca tggtgttatt gaaaccccaa tttttatgcc tgttggaacg
     1861 gttgcttcgg taaaaggagt gcaccaacgc gaactgaaag aagaaataaa cccagatatt
     1921 atcttgggga atacctatca tttatacttg cgtccgcaaa ccgaaatctt ggaaaaagcg
     1981 ggtggattgc ataaatttat gaattgggac agaaatattt tgaccgattc tggtggatac
     2041 caagtgtatt cgctttcttc taaccgaaaa attaaggaag aaggggtgaa attcaaatcg
     2101 catattgatg gttcctacca cgtttttaca cccgaaaatg taatggaaat tcagcgtacc
     2161 atcggtgctg atatcattat ggcttttgac gaatgtacgc cttatccgtg cgattatcgt
     2221 tatgcgcaac gttcgatgca catgacgcac cgttggttgg acagatgcat caatcatttg
     2281 gaaaaagtgc cttttaaata tgggtatgaa caaacttttt ttccgattgt tcaaggaagt
     2341 acctacaaag atttacgccg tcaatcggcg gaatatattg ctaattcagg tcaacaagga
     2401 aatgctattg gaggactttc agtaggagaa ccagccgagg aaatgtatgc gatgaccgaa
     2461 gtggtttgtg aaattctgcc agaagacaaa cctcgctatt tgatgggagt aggaactccg
     2521 attaatatct tggaaaatat tgccttagga attgatatgt tcgattgtgt gatgccaact
     2581 cgaaacgcta gaaacggaat gttgtttact gccaatggaa ccatcaatat caaaaataaa
     2641 aagtgggaag acgatttctc ggtaattgac gaaatgggcc atacttttgt ggatacagaa
     2701 tacagcaaag cttatttgcg ccacttattt gccgccaatg aataccttgg aaaacaaatt
     2761 gccacgattc acaatcttgg gttttatatg tggttggttc gtgaggctag aaaacatatc
     2821 ttagccggag attttagaac ttggaaagag atgatggttc gcaatatgag ccagagactt
     2881 taaaataatt atgaatttta aattatgaat tatgagttag ctcgtagttc ataattcata
     2941 attcataact cataactcat aattcaaaat atgctgacaa taatagacaa atacattcta
     3001 aagaaatatt tggccacatt tggggttatg ttgggtttat ttgcacctat tggaatagta
     3061 attgatattt cggaaaagat taataaaatg atcgaaaaca agattcccat tatggaaatt
     3121 ttggtttact attatcattt tacaatttat tttatgaatt tgttgtttcc gatattcttg
     3181 tttttatcgg tcatttggtt tacttcaaaa ttagccaatg atactgagat tattgctatt
     3241 ttaagttcag gaatttcgtt tactcggttt ttgagacctt ttatgattgg agctactatt
     3301 gtttcgttaa tcgttttgtt gatggggttt tttatcgttc caaaatcgaa tgaaggtttt
     3361 aataatttta gatatactta tcttcgtggt gatggtaaag aaactgtaag gggaaaaaat
     3421 acagatgttt tccgtcagct tagcaatgat gaatacatct atgtcaatag ttttgatgta
     3481 gcatctaaaa cggcttataa tttcacttat gaaaaattca ccaaagataa attagcttat
     3541 aaaatttcgg cttcaaggat acagtggaat cctaaaacta aaaattatac gatgtatgat
     3601 tatgtcaaac gaactgttgg aggattggat gataaaattg aaaaatcgga taaaaaagat
     3661 gctaaattta gttttgatct cgacgatata acaccagtta tttatatagc cgaaaccttg
     3721 agtttgggta agttaaacca atttatcgaa aaagaaaaaa agcgtggttc ttcaaacatc
     3781 aatgtttata aggtcgtttt gtataaaaag tacagcatac ccgtatcggc atttattctt
     3841 actgtaattg cagttg
//
LOCUS       DAOSNN010000247         3822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_23977_length_3822_cov_5.333422, whole genome shotgun sequence.
ACCESSION   DAOSNN010000247 DAOSNN010000000
VERSION     DAOSNN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23977_length_3822_cov_5.333422"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1180
                     /gene="mgrA"
                     /locus_tag="PLQ70_10740"
     CDS             191..1180
                     /gene="mgrA"
                     /locus_tag="PLQ70_10740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HQE35135.1"
                     /translation="MTYQPATNRYDQMEYRRCGNSGIKLSAFSLGLWHNFGHNSDFEN
                     SRNLIKTAFDNGITHFDLANNYGPPPGSAEECLGKILKLDFKNHRDEMIISSKAGYTM
                     WDGPYGDWGSKKYLVSSLDQSLKRMDLDYVDIFYHHRPDPETPLEETMAALNLIVQQG
                     KALYVGISNYRPAEAEKAFKILKEMGTPCLIHQPKYSMFERWVEDGLLDLLGKEQVGC
                     IPFSPLAQGLLTDKYLNGIPADSRVATSGQFLNAKHITPEVLEKIFALNNIAKERGQK
                     LAQMALAWILRDPRVTSVLIGSSKPEQIIDSIKALENTSFSKEELNKIDLILK"
     gene            1195..3546
                     /locus_tag="PLQ70_10745"
     CDS             1195..3546
                     /locus_tag="PLQ70_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQE35136.1"
                     /translation="MENLKKTEAKRFRFSFILILVFATAAVFSQGRKTINFDANWQFT
                     KGEIAGAEKPTFNDKAWRTLDVPHDWSIEGAYDRENPSARGGGYLPTGIGWYRKTFQL
                     DATDANKILTIVFDGVMANSDVWINGKHLGKRPYGYISFTYDLTPYLNFGKEKTNVIA
                     VRADNSVQPASRYYTGAGIYRHVRLVAVNPTHFKHWGTQITTPNATAQKAVVSVKTEI
                     ENKGAAGTYKLQIDIVDSKGKIVKSSESQENIAANTTSNLVKEMEVQNPKLWDIEAPN
                     LYTVTTKLYSGKTLIDNETTTIGIRNAEFRADTGFWLNNKNYKIKGVCLHHDGGAVGA
                     AVPLGVWKERLKKLKEVGVNGIRTSHNPVAPEFLDLCDQMGFVVMDETFDTWTAAKHN
                     GEKGYNLIFKEWWEQDTKDMVLRDRNHPSIVIYSIGNEVHDDLSYPEGFKTYKMQEDV
                     VKKQDNTRPVTMALFRPANSKVYLSGFADHMEIVGQNYRENELIAAHEAHPTWKVIGT
                     ENTHVLVQWLALRDKPYMAGQFLWTGYDYLGEADWPETTNNQGLFDRAGNWKQQSLQR
                     DSWWSEEPVVHIVRKSDNAGAGVWVADWTPNDFDTYDNAKVNVYSNCEEVELFLNEKS
                     LGALKKPADDSPREWNVTFEAGTIKAIGKNNGKIVAQEEQTSAGKPAKIILTKSIPTL
                     ANNWDDVTFITATIVDEKGIRCANADNQIKFTITDAGKIIAVDNGNIINHDGYQGPAT
                     RAYQGKAIAIVKATKDSGTITIKAETEGLGHGSIPIEIVAEKK"
BASE COUNT         1350 a          656 c          787 g         1029 t
ORIGIN      
        1 cagagcacct cgtttacacc gagggggtcg ggggttcgaa cccctcatcg cccaccaaga
       61 aacttcaaca gaaatgttgg agtttttttt tgcccaaata cttccacaca acttttattt
      121 taggattgtt ttaaaaagtt ctaaaaaaaa tgaaatatct tcgtaaacga ttacgaaaaa
      181 tctataaaaa atgacttacc aaccagcaac aaacagatat gaccaaatgg aatatcgcag
      241 atgtggaaac tcaggaatta aactttcggc tttttcatta ggattatggc acaatttcgg
      301 tcacaacagt gattttgaaa acagcagaaa cttgataaaa accgcttttg ataatggaat
      361 tacccatttt gatttggcta acaattatgg gccacctcct ggatcggcag aagaatgtct
      421 ggggaaaatt ttaaaattag atttcaaaaa ccacagagat gaaatgatta tttcgtcgaa
      481 ggcgggttat acgatgtggg atggccctta tggcgattgg ggttcaaaaa aatatttggt
      541 ttccagtcta gaccaaagtt taaaaagaat ggatttggac tatgtagata ttttttacca
      601 tcacagacca gacccagaaa cgcctttgga agaaactatg gcagcattga atttaattgt
      661 gcaacaagga aaagcgttgt atgttggtat ttcaaactac cgacctgccg aagccgaaaa
      721 agcgttcaaa atattaaaag aaatggggac accatgccta attcaccaac ccaaatattc
      781 gatgtttgaa cgttgggtcg aagatggact tttggatttg ttaggaaaag aacaagtggg
      841 ttgcattccg ttttcgcctt tggcacaagg attattaacc gataaatatt taaacggaat
      901 tcctgctgat tccagagtgg ctaccagcgg acaatttcta aacgccaagc atattactcc
      961 cgaagtatta gaaaaaatat tcgctttaaa caatatcgca aaagaaagag gtcaaaaatt
     1021 agcccaaatg gctttggctt ggattttacg tgacccaaga gtgacttcgg tattaattgg
     1081 ttcaagcaaa cccgaacaaa ttatagattc tatcaaagct ttagaaaaca ccagtttcag
     1141 caaagaagaa ttaaacaaaa ttgatttgat tttaaaataa caaacacaat tataatggag
     1201 aatctcaaaa aaacagaagc aaaacgattt agatttagct tcatattaat tttagtattt
     1261 gctacagcag cggtcttttc gcaagggcga aaaacaatca attttgacgc taattggcaa
     1321 tttacaaaag gagaaatagc tggagccgaa aaacctacat ttaatgacaa agcatggaga
     1381 acactagatg ttccgcacga ttggagtatt gaaggggctt atgatcgtga aaatccgtcg
     1441 gcacgtgggg gaggttattt gccaacagga attggttggt atcgcaaaac atttcaatta
     1501 gacgccactg atgctaacaa aatactaacc atagtatttg atggtgttat ggccaacagc
     1561 gatgtatgga ttaacggaaa acatttagga aaacgtcctt atggctacat cagttttacg
     1621 tatgacctta cgccttattt gaactttgga aaagaaaaaa ccaatgtcat tgcggttcga
     1681 gctgataatt ctgtccaacc tgcttctcgt tattatactg gtgctggaat ttatcgtcac
     1741 gtacgtttgg tagctgtaaa tccaacacat ttcaaacatt ggggaacaca aattacgact
     1801 ccaaatgcaa ctgcacaaaa agcggttgtg agcgtgaaaa ctgaaataga aaacaaagga
     1861 gcagctggaa cttataaact gcaaattgat attgttgaca gcaaaggaaa aattgtaaaa
     1921 tctagcgaaa gtcaagaaaa tattgcagcc aatacaacca gcaatttagt caaagaaatg
     1981 gaggttcaaa atcctaaatt atgggatatt gaagcaccca atttatatac tgtaactacc
     2041 aaattgtatt ccggaaaaac gcttattgat aatgagacga ctaccattgg aatcagaaat
     2101 gctgaattta gagccgatac tggtttttgg ttaaacaata agaattacaa gataaaagga
     2161 gtttgcttgc atcacgatgg tggagcggtt ggcgctgcag ttccgcttgg agtttggaaa
     2221 gaacgcttga aaaaattaaa agaagtgggc gtaaacggca ttcgaacttc acacaatcct
     2281 gtcgctccag aatttttgga tttatgcgat caaatgggat ttgtggtgat ggacgaaact
     2341 ttcgacactt ggacagcggc caaacacaat ggcgaaaaag gctataattt aattttcaaa
     2401 gaatggtggg aacaagacac caaagacatg gttttaagag accgaaacca tccttcgata
     2461 gttatttata gcattggcaa cgaagttcac gatgacctta gttatcctga aggctttaaa
     2521 acctataaaa tgcaggaaga tgttgttaaa aaacaggaca atactagacc tgtaactatg
     2581 gcacttttca gacccgccaa ttctaaagtt taccttagtg gctttgccga tcatatggaa
     2641 attgtgggtc aaaattatcg cgaaaacgaa ctgatagctg cacacgaagc gcatccaact
     2701 tggaaagtta ttggcaccga aaacactcat gtacttgttc aatggttggc tttgagagac
     2761 aagccctata tggcaggaca atttttatgg acgggttacg attatttagg cgaagccgat
     2821 tggccagaaa ccactaacaa tcaaggttta tttgacagag ccggaaactg gaaacaacaa
     2881 tctttgcaaa gagacagttg gtggtctgaa gagcctgtgg tgcacattgt gagaaaatcg
     2941 gataatgcgg gagctggagt ttgggttgct gattggacgc cgaatgattt tgacacttac
     3001 gacaatgcca aagtgaatgt ctatagcaat tgtgaggaag ttgaactttt tttgaacgag
     3061 aaatcattag gtgccttaaa aaaacctgcc gatgattctc caagagaatg gaatgttact
     3121 tttgaagctg gaacaatcaa agccattgga aaaaacaacg gaaaaattgt tgctcaggaa
     3181 gaacagactt ctgctggaaa acctgctaaa attatcttaa ccaaaagcat tccaacttta
     3241 gctaacaatt gggatgatgt gacatttatt accgcaacga ttgtggacga aaaaggaatt
     3301 agatgtgcca atgccgacaa tcaaattaaa tttacgatta cggatgctgg caaaattatt
     3361 gcggtcgata atggaaacat tatcaatcac gatgggtatc aaggtccagc aacaagagcc
     3421 tatcaaggaa aagcaattgc cattgtaaaa gcaaccaaag acagcggaac cattaccata
     3481 aaagccgaaa ccgaaggttt aggacatggt tccatcccga tagaaattgt ggctgaaaaa
     3541 aaataatgat aaaccgtaag gtttaaatat caaaaacgtc agttcgagtg ttttataaaa
     3601 aatgcgaaag ctttttttat aaaatgtatc gagaaccgtt ctttaaagtc aggaaaaaaa
     3661 gtgatttttt ttaacagaaa aaggtattct agcaatagag tacctttttt gcttttgttt
     3721 gacactccat ttgttttgaa tatttataac aaaatactca cataagatga aagatttttt
     3781 atacattagc tgaatacgaa aaaagctgat ttgtagagag aa
//
LOCUS       DAOSNN010000248         3799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_24175_length_3799_cov_4.914797, whole genome shotgun sequence.
ACCESSION   DAOSNN010000248 DAOSNN010000000
VERSION     DAOSNN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24175_length_3799_cov_4.914797"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1062)
                     /locus_tag="PLQ70_10750"
     CDS             complement(19..1062)
                     /locus_tag="PLQ70_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQE35137.1"
                     /translation="MNWKAKIITHRGSKRIAVFFEKNAELIARIKKLEGSRWSQTLGV
                     WHLPDTEENRIRFKIAPLTHSIPSTEGMAQIEKFKQWLRSKRYSESTITTYSEALKSF
                     LVFYRNKAIIDITNEDVIVYNNEHILKNNFSASFQNQTVNAIKLFFQTIRDTKMMVDK
                     IHRPKRAKVLPNVLSKEEIKLILNAHSNIKHKAMLSMIYSCGLRRSELLHLRFSDIDA
                     KRNIVLLKNAKGKKDRIAPLSPKILQMLREYYINYKPAVWLFEGQIKGEQYSEKSLQS
                     VLKQALQKTGITKPVTLHWLRHSYATHLLESGTDLRYIQELLGHSSSKTTEIYTHVST
                     KSIQQIKSPFDDL"
     gene            1254..1691
                     /locus_tag="PLQ70_10755"
     CDS             1254..1691
                     /locus_tag="PLQ70_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35138.1"
                     /translation="MKINLKNGIDKLLFGMKQNDVTALYGKPNRNYKDEDDNVIFAYN
                     ALKMRLTFYQEEDLKLGYIVASSADLELFGNTIIGKKITEVKKELATKGITKYNQEDF
                     DTFENYFNEDNWFILQTEFDEVVKFEIGAIINAKDEFDWKFKK"
     gene            complement(1815..3029)
                     /locus_tag="PLQ70_10760"
     CDS             complement(1815..3029)
                     /locus_tag="PLQ70_10760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQE35139.1"
                     /translation="MKSKILVLLFLGIVNLQAQTAKKPVTATKKPTATTKTAPKATSP
                     AAAEGIFATIATSKGNIVLQLEYKKTPVTVANFISLAEGTNTFVTDAKLKGKPFYAGL
                     KFHRVIADFMIQGGDPLGTGSGDPGYSFKDEITDAKFDKAGILAMANSGPATNGSQFF
                     ITHKDTPWLTGKHTIFGYVIQGQNVVNAIAQNDLILKVTIARKGTAAKAFDAPKVFST
                     YFLNKDEDAKKQALIDAENQKKQAEAEALAKKEYEVKYGSVIKAKAAYFANEKTTGTT
                     TESGLIYKITKKGSGVKPANGTSVNINYAGYFEDGNLFDSNYEEVAKTYGKYDPNRAA
                     QGGYNAFPFEIGKQGMIPGFLEGLNLLSFGDKALIFIPSKLAYGEAGAGGVIPPNATL
                     IFEIEMNEIKAK"
     gene            complement(3073..>3799)
                     /locus_tag="PLQ70_10765"
     CDS             complement(3073..>3799)
                     /locus_tag="PLQ70_10765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQE35140.1"
                     /translation="NNGPATNGSQFFITHLETPWLEGKHTIFGQVVENGMDVVNKIVQ
                     GDYMDKVTIIRNGEAAKKFDAIKVFRDYYTVEAENQKNKLAIEAAENAKYKAIFDQKT
                     TLFEALKTKAITTPTGLAYTITKKAGGKKPKAGATVYIHYAGYLENGSLFDSSIEAIT
                     KIYGKYDPNRGAQNGYQPIPFQYGKKDGLIPGFIEGIEQLSFGDKATLFIPSKLGYGE
                     AGANGVIPPNANIIFEIELLEKI"
BASE COUNT         1089 a          659 c          721 g         1330 t
ORIGIN      
        1 aaatataata attttttctt acaaatcatc aaaaggactt tttatttgtt gaatactttt
       61 tgtactaacg tgcgtgtaaa tttcggtagt tttactgctg ctatgtccta atagttcttg
      121 tatatacctc aaatcggtgc cactttctaa taaatgagta gcgtaactat gtcttaacca
      181 atgcaaggtc accggttttg ttatgcctgt tttttgtaga gcttgtttca agacgctttg
      241 taaacttttt tcagaatatt gttcgccttt aatttgtcct tcaaataacc acactgctgg
      301 tttgtagttt atataatatt ctcgtagcat ttgcaaaatt tttggagata atggtgcaat
      361 tcggtctttt ttgcctttgg catttttgag caaaactata tttcttttgg catcgatatc
      421 cgaaaaccta agatgcaata actcactacg acgcaaccca caactgtaaa tcatagatag
      481 cattgctttg tgcttgatgt tgctatgggc attcaaaatc aattttattt cttctttact
      541 caatacatta ggtaaaacct tggcgcgttt gggtcggtgt attttatcga ccatcatctt
      601 agtatcacga atggtttgaa aaaagagttt aatagcatta acggtttggt tctgaaaaga
      661 agccgaaaaa ttgtttttca atatatgttc gttattatag acaatcacat cctcattggt
      721 aatatcgatt atggctttgt tacgataaaa aaccaagaag gattttaggg cttcgctata
      781 agtagtaatc gtactttcgc tatagcgttt ggatcgcagc cattgcttga atttctcgat
      841 ttgtgccatt ccttcggtcg atggaatgct gtgtgttaaa ggggcgattt taaaccgaat
      901 ccggttttcc tctgtgtctg gaagatgcca aacgcccaga gtttgactcc agcgagagcc
      961 ttccagcttt tttattcgag cgattaattc ggcattcttt tcgaaaaaaa cggcgattct
     1021 tttgctgccg cggtgcgtga tgattttggc tttccagttc ataatcagtg gttttgaacg
     1081 actaaaataa ggaattgttt ttaatttaaa aacgtgatgt actttgcaaa aaactttgcg
     1141 aactttgcgg taaaatacaa tcgaatgaaa tgagcgtaac gaaaatttga taaaaagacg
     1201 aatattgata tgcgaattac atttgaccga atgaaaacaa taaaaaacaa caaatgaaaa
     1261 taaatttaaa aaacggaatc gacaagctcc ttttcggaat gaaacaaaac gatgtaacgg
     1321 cgctgtacgg aaaacccaac cgcaattaca aggacgaaga cgacaacgtg atttttgctt
     1381 ataatgccct aaaaatgcgt ttgactttct atcaagaaga agatttaaaa ctaggatata
     1441 tagtagcttc gagtgcagac ttagaattgt ttgggaatac aattattggt aaaaaaatca
     1501 ccgaagtcaa aaaagaattg gccaccaaag gcattactaa atacaatcaa gaggatttcg
     1561 atacgttcga aaattatttt aatgaagaca attggtttat tctacaaacc gaatttgacg
     1621 aagtggtgaa atttgaaatt ggagccatta tcaatgccaa agacgaattt gattggaagt
     1681 ttaaaaaata atcgtagaga cgcaatgtat tgcgtttctg tattcaagac accacctaaa
     1741 acaaacaaac cccaaccatt ttgaattggt tggggtttgt tgttgtaata aaataataac
     1801 gttttgtata ttgtttattt tgctttaatt tcgttcattt caatttcaaa aataagggtt
     1861 gcgtttggcg gaatgacacc accagctcca gcttcgccat aagctagttt cgatggaatg
     1921 aagatcaaag ctttatcacc aaaagaaagc aggtttaatc cttctagaaa tccaggaatc
     1981 attccttgtt tgccaatttc aaaaggaaag gcattgtatc cgccttgagc ggctctgttg
     2041 ggatcatatt ttccatacgt tttggctact tcttcataat tgctgtcaaa caagttccca
     2101 tcttcaaaat aacctgcata gttaatgttg accgaagttc cgttggcagg ttttactcca
     2161 gatccttttt tggttatttt ataaatcaac cctgattctg ttgtagttcc agtagttttt
     2221 tcattggcaa aataggctgc ttttgcttta attacactac cgtattttac ttcatattct
     2281 tttttggcta atgcctcggc ttcagcttgt tttttctgat tttcagcatc aatcaaagct
     2341 tgttttttgg catcttcatc tttattcaaa aagtaggttg agaatacttt tggagcgtca
     2401 aatgctttgg cagcagttcc ttttcgggca atggttactt ttagaatcag atcattttga
     2461 gcaatcgcat tgactacatt ttgtccttga attacgtagc caaaaatagt gtgtttgcca
     2521 gttaaccaag gggtgtcttt gtgtgtaata aagaattggc taccattagt agcaggacca
     2581 gaattggcca tagccagtat gcctgctttg tcaaatttag catcggtgat ttcatcttta
     2641 aatgaatagc ctggatcgcc agaacctgtt cctagaggat cgccaccttg aatcataaaa
     2701 tcagcgatta cacggtgaaa ttttagtccg gcataaaaag gttttccttt tagttttgcg
     2761 tcggttacaa aggtgtttgt accttcggca agcgaaataa aattcgctac tgtaacgggt
     2821 gtttttttgt attctaattg caaaacgata tttcctttgc tggtagcaat agtggcaaaa
     2881 attccttcgg cagctgctgg tgaagttgct ttgggtgcag ttttggtggt tgcagtaggt
     2941 tttttggttg ctgtaactgg tttcttggcg gtttgtgcct gaagatttac tattcctaaa
     3001 aacaatagga ctaaaatttt agatttcatt tttaataatt taatgttcgt ttttcgtaat
     3061 tctaacttgt aattatattt tttctaacaa ttcaatttcg aaaataatat tggcattggg
     3121 cggaataact ccattagctc ctgcctcgcc atagcccaat tttgatggga taaaaagtgt
     3181 ggctttgtcg ccaaaagaca attgctcaat tccttcaata aatccaggaa tcaaaccgtc
     3241 ttttttgcca tattgaaaag ggatgggttg ataaccattt tgagcaccac ggtttgggtc
     3301 atattttccg tagatttttg taatggcttc aatactggaa tcaaaaaggc ttccgttttc
     3361 aagataacct gcatagtgaa tgtaaacagt ggctcccgct tttggttttt tgcctcccgc
     3421 ttttttggtg atggtatagg ctaaaccggt tggtgtagtg atagctttgg tttttaaggc
     3481 ttcaaataaa gtggtttttt ggtcaaaaat agctttatat tttgcgtttt cggctgcttc
     3541 tatcgccaat ttattttttt ggttttcagc ttctacggta taataatctc tgaatacttt
     3601 tatggcatca aattttttgg ctgcttcacc atttctaatg atggtaactt tgtccatata
     3661 atcgccttgt acgattttgt tcactacatc cattccgttt tcgacaacct gaccaaaaat
     3721 ggtgtgtttg ccttctagcc aaggtgtttc taaatgcgtg ataaaaaact gactgccatt
     3781 ggttgctgga ccattatta
//
LOCUS       DAOSNN010000249         3779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_24340_length_3779_cov_5.923469, whole genome shotgun sequence.
ACCESSION   DAOSNN010000249 DAOSNN010000000
VERSION     DAOSNN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24340_length_3779_cov_5.923469"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..723)
                     /locus_tag="PLQ70_10770"
     CDS             complement(121..723)
                     /locus_tag="PLQ70_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HQE35141.1"
                     /translation="MKKLLLVLALVSMGYTNAQTRNKGTMEITPKIGSSSFYENSEYD
                     SSKYNSGVQFGVTADYYFNNRWSLRSGLIFDKMGGTNTDAEDKYEDILNYLSIPINAN
                     WHFGSTRKWNLNFGLSPSFLTKATAKYNGTSEKIPSSAIESFQLGFTYGIGYKIGITE
                     KFGILIDAQFFNGLTNINKATDFRVVNAGYSFNLGGVIQL"
     gene            complement(874..2256)
                     /locus_tag="PLQ70_10775"
     CDS             complement(874..2256)
                     /locus_tag="PLQ70_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQE35142.1"
                     /translation="MKNKALVLILFLSFQSLFSQEIQGSWAGSLSIQGTQLPLIFNIQ
                     KNGDSYKTTLDSPMQGAKGIPVAETTFANNQLQIVAQNLNLKFSGLLKEEAIEGTFIQ
                     NGASFPLVLTRKKEDTPALNRPQEPKAPFDYPIEEVVFVNAKDKNTLAGTLTLPKNKK
                     DFPVVVLITGSGAQNRDSEIFGHKPFAVIANDFAQKGIGVLRLDDRGVGGSSKGTTED
                     TSANFATDISAAVDFLSAKGFGKIGLIGHSEGGLIAPIVAVQNKKVQFIISLAGPGIP
                     IDQMMLLQSQAIAKSQGATPAQIEASTAFNQKVYAYIINYKGADLKTDFQPFVKEEFK
                     KITQSQGLSESQIDNFVAQQTQNITSSWYVYFIKFNPDLYWSKLKIPVLALNGSLDVQ
                     VKATENLAGIQASLSKAGNKKVAIKELPGLNHLFQEAKTGATAEYLTIEQTFAPLVLD
                     TMSNWILGIK"
     gene            complement(2442..2624)
                     /gene="tatA"
                     /locus_tag="PLQ70_10780"
     CDS             complement(2442..2624)
                     /gene="tatA"
                     /locus_tag="PLQ70_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQE35143.1"
                     /translation="MGRLGVTEILVILAVVLLLFGGKKIPELMKGLGSGIKEFKNAAK
                     DDQPADKKETKEETKE"
     gene            2830..3330
                     /locus_tag="PLQ70_10785"
     CDS             2830..3330
                     /locus_tag="PLQ70_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016988976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HQE35144.1"
                     /translation="MSENRLKRRKLHHKLFTKNRLVILNEETFEELFSFKLNLMNVFV
                     AATSGAILLITFTTFIIAFTPLREFIPGYSSSKLKKDATELALKSDSLTLALQQNEAY
                     IKSIQKVLTGELEYAKFNKDSILASASDTVSKADLSPSKEELELRKEVAKEEKNTKSN
                     ASKAKK"
     gene            3340..>3779
                     /locus_tag="PLQ70_10790"
     CDS             3340..>3779
                     /locus_tag="PLQ70_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HQE35145.1"
                     /translation="MSLKSFFAKIFAQQIYRKTQLWATNPIATQQQVFEDLIRQAKNT
                     QFGIDHHFDQIKTFEDFSKNVPVRDYEGLKSYVDKVVKGEENVLWKGKPIYFAKTSGT
                     TSGAKYIPLTKESMPFHIEAARNAILHYIHETGKANFVDGKMIFL"
BASE COUNT         1288 a          666 c          581 g         1244 t
ORIGIN      
        1 tgaggtaaaa gtaacagagg ttgatgagga tagaaagaga attcagttga cgatggtttt
       61 gtaaaataaa aaagccagtt tcaagatttt gagactggct atacttttag aattactatt
      121 ttataactga attacaccac caagattgaa gctgtaacca gcattgacta cacgaaaatc
      181 tgttgcttta tttatattag tcaaaccatt gaagaattgt gcatcaatta aaattccgaa
      241 tttttctgta atcccaattt tataaccaat accatatgta aatcctaact gaaaagattc
      301 aatagctgat gaaggaattt tttcagatgt accattatat tttgccgttg cttttgttaa
      361 aaaagaagga cttaagccaa aattcaaatt ccattttcgt gtgctaccaa aatgccaatt
      421 ggcattgatt ggaatgctta aatagtttaa aatatcctcg tatttatcct cagcatcagt
      481 atttgttcct cccattttat caaatatcaa acccgaacgt aaactccacc tattgttaaa
      541 ataataatca gcagttactc caaattgtac acctgaattg tatttagagg aatcatattc
      601 actattttca taaaaacttg agcttccaat ttttggagta atttccatag ttcctttgtt
      661 tctagtttga gcatttgtgt atcccattga tactaaagct agtactaata ataatttttt
      721 catttcttaa attttaaaaa taatactaca aaaataaaaa ttttagttga aaaagaaaag
      781 aaaaaaatcg ataatttaag attgataaat ctattttaga agatagagta aaatgaattc
      841 agttgacaat ggttttgtaa aatttttcat cttctatttt atccccaaaa tccaattact
      901 catcgtgtct aaaaccaaag gagcaaaggt ttgttcaata gttaaatatt cggctgttgc
      961 tcctgttttg gcttcttgaa acaaatgatt taatcccgga agttctttta ttgcaacttt
     1021 tttatttcct gctttggata atgaagcctg aattcccgct aaattttcag ttgcttttac
     1081 ttgaacatct aatgaaccat ttaacgccaa tacaggaatt ttcaacttag accaatacaa
     1141 atcgggattg aattttataa aataaacata ccacgaactt gtaatgtttt gagtttgttg
     1201 cgcaacaaaa ttatcgattt gactttctga aagaccttga ctttgagtta tttttttaaa
     1261 ttcttcttta acgaatggtt gaaaatcagt ttttaaatcg gctcctttat aattgataat
     1321 ataggcatat actttttggt taaaagcagt actagcctca atttgagcgg gagttgcacc
     1381 ttgcgatttt gcaattgctt gactttgcaa aagcatcatt tggtcaattg gaatgcccgg
     1441 accggcaagc gatatgataa attgtacttt tttgttttga accgctacaa tgggtgcaat
     1501 taatcctcct tcgctgtggc cgattaaacc aatttttcca aaaccttttg cactcaaaaa
     1561 atcgacagct gcgctgatat ctgtagcaaa attagccgaa gtatcttctg tagttccttt
     1621 gctagaaccg ccaacacctc gatcgtctag acgcaatact ccaattcctt tttgagcaaa
     1681 atcattagca atgacagcaa aaggtttgtg accaaaaatt tcggaatcgc gattttgagc
     1741 acctgaacct gtaattaaaa ctactacagg aaaatctttt ttgtttttag gaagtgtcaa
     1801 agttcctgcc agtgtatttt tgtcttttgc attcacaaaa accacttctt ctattggata
     1861 atcaaaagga gctttgggtt cttgcggacg atttagtgct ggagtatctt cttttttcct
     1921 agttaatacc agtggaaaac tagccccatt ttgtataaaa gtcccttcga tggcttcctc
     1981 ttttaagaga ccactaaatt tcaaattgag attttgtgcc actatttgca attgattatt
     2041 agcaaaagtt gtttctgcaa caggaatccc tttggcacct tgcatggggc tgtctaaggt
     2101 agttttatag gaatctccat ttttttgaat attaaaaatc agcgggagtt gagttccttg
     2161 aatacttaaa cttcccgccc aagagccttg aatttcttga gaaaaaagcg actggaatga
     2221 taaaaaaagg attagtacta aagctttgtt tttcattgga taaggacttt attaaaaata
     2281 aaactctaag gtaatcgaag ctagattaaa acccaaataa ttttgacaaa taacgtttct
     2341 aagaatgcaa aagcagtaat agttataaaa acaaaatccc aaaccccaaa cagattaatt
     2401 gtttggaatt tggaatatta aattttaaac ctaaagaaaa attattcttt ggtttcctct
     2461 ttagtttctt ttttatccgc cggttggtcg tcttttgcag catttttgaa ttctttgatt
     2521 ccgctaccta aacctttcat taattctgga atttttttac ctccaaaaag taataaaaca
     2581 accgccaaaa taacaaggat ttctgttaca cccaatcttc ccataatata aaattttatt
     2641 gccgaagcaa atttctaata aatcgcgcaa caaaggtata tagaattact taacataaaa
     2701 tcaattaggg ataattattt tagtttctta tacgttaaaa atttggtaaa aaagccaaga
     2761 cacaaacaca aacaagaaaa caagcgaatg attgcattaa ttattatatt tgtatcataa
     2821 acgcaaaata tgtctgagaa taggcttaaa agaagaaaac ttcaccataa attattcaca
     2881 aaaaaccgtt tggttatttt aaatgaagaa acatttgaag aacttttttc cttcaaattg
     2941 aatctgatga atgtttttgt ggccgcaact tcaggggcta ttttattgat tactttcacc
     3001 acttttatca ttgcttttac ccctttgaga gagtttattc caggatattc ttcttctaaa
     3061 ttaaaaaaag acgctaccga attagctctt aaatcagatt ccttaacttt ggctttacag
     3121 caaaatgaag cctacatcaa atctattcag aaagtattga caggcgaatt agaatatgcc
     3181 aaattcaata aagactctat tcttgcctca gcctctgaca cagtttctaa agcggattta
     3241 tctccttcaa aagaagaatt ggaattaaga aaagaagtcg ccaaagaaga aaaaaacacg
     3301 aaatccaacg catcaaaagc gaaaaaataa aaccatacaa tgtctttaaa atcttttttt
     3361 gctaaaatct ttgctcaaca aatttatcga aaaacacagc tttgggcaac caatcccata
     3421 gcaacgcagc agcaagtttt cgaagattta atccgtcaag ccaaaaacac tcaatttggc
     3481 attgaccatc atttcgacca aataaaaacc tttgaagact tttcgaaaaa tgttcccgtt
     3541 cgcgattacg aaggtttgaa atcctatgtg gataaagttg tgaaaggcga ggagaacgtg
     3601 ctctggaaag gcaaaccaat atattttgcc aaaacttcag gtacgacttc gggagccaaa
     3661 tacattccac tcaccaagga atcgatgcca tttcatatcg aagcggctcg aaacgccatt
     3721 ttgcattaca ttcacgaaac aggcaaggcc aattttgttg atggcaaaat gattttcct
//
LOCUS       DAOSNN010000250         3737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_24684_length_3737_cov_4.318305, whole genome shotgun sequence.
ACCESSION   DAOSNN010000250 DAOSNN010000000
VERSION     DAOSNN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24684_length_3737_cov_4.318305"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /locus_tag="PLQ70_10795"
     CDS             <1..1721
                     /locus_tag="PLQ70_10795"
                     /inference="COORDINATES: protein motif:HMM:NF026034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide lyase family protein"
                     /protein_id="HQE35146.1"
                     /translation="TYSILKHKATYPNFDLGSWRQVLWINSSVTNKICVNELKTWEMP
                     QPGDAWSPTSIAEIIKIESGVRAGKYDGKYQFSEPLYSLKAYGHSSDTNNLGIWAVTG
                     NHEYFSGGPTSHDLNSASGIIHILMNGVHYNSTGFQVPLGEEWSKIYGPYLIYANQNA
                     TATANWDEAKARAAVDKTEWPFSWLTNTPDYPLAAGRGNIIGNFNITDPSKTNVNGNE
                     AWIGVTQLSPDAGGNWQFEEKNYQYWVQTDASGNFDIKNVRPGTYTLFAYKNGTTGEY
                     RQETVVVTAAATTNLGNINWTIPRTNGNLVFEIGVPNRTAEEYKFGDFDYCEGFVENK
                     FAATFTNPIEYNVEDKNWPTALSYVHSAYFNADGTRSVWDWNVNFNLTGTIPTTGNAK
                     LTIAYASSDHAQNWIFINGSRVTPSSGYYPPNGGGNAFLRQSNHAKYGLATFDIPYSK
                     LKSGKNTLKLQMPSTSSGGNHVMYDYISLEGDLTTLGINSKGVDVADFRMYPNPTKGI
                     FEIALPLSIQETTIELYTMNMQLISKKAYTPVNGKVQLNIENQANGVYFAKIGTTGVT
                     FKVIKE"
     gene            complement(1794..2318)
                     /locus_tag="PLQ70_10800"
     CDS             complement(1794..2318)
                     /locus_tag="PLQ70_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE35147.1"
                     /translation="MATIEITKATLSHLDQLQKIGRQTFEETFSESNSEENMKNYLEE
                     GFSNEKLTLELKDQNSEFYFATLDNEVIGYLKINFGASQTELKDSKALEIERIYVSKD
                     FHGKSVGQLLYDKAIQIAKQKNVDYVWLGVWEENPRAIRFYKKNGFVEFDKHIFKLGD
                     DEQTDIMMKLYLNN"
     gene            complement(2333..3730)
                     /locus_tag="PLQ70_10805"
     CDS             complement(2333..3730)
                     /locus_tag="PLQ70_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQE35148.1"
                     /translation="MNNFNSKIGFTFTLLFFFLVSFGQKKQVKSIEKPNIILIIADDA
                     GWNDVGYNGSEIQTPNIDRLAKNGVILNRFYANPTCSPSRASLLTGKPASRMGIVAPI
                     SDKSTLKLPDSIPTLPQLLHQNNYETALFGKWHLGLQPSSGPKAYGFDYSYGFLHGQI
                     DQYTHKYKNGDESWYRNGEFIVEKGHATDLITQEAIQWISEKRDSKKKFFLQVAYSAP
                     HFPLQEEQKWKDPYMTSIKNSSRRDYAAAMSHLDNSIGLLLDKLKQENLDKNTLIVFM
                     SDNGAMENWDSKGEYNDEHPSNDRLGDNTPLRDWKTSNYEGAIRVPCVFYWKGQLVHY
                     KNEGYISIIDVLPTFLFLAGDKKLPKTVEGKNAWSVISEHDPDSYKDLEIPNQEIYVR
                     GHLQESLIQSPWKIIRTRHLTIPADYELYNIEKDPSEKNNVILQNQTLAEKMKIALEN
                     QFKKDAKEVNVNGIK"
BASE COUNT         1166 a          679 c          662 g         1230 t
ORIGIN      
        1 atacctactc tattttaaaa cataaagcca cttaccccaa ttttgattta ggttcttgga
       61 gacaagtatt atggataaat agtagtgtta caaataaaat atgtgtaaat gaattaaaaa
      121 catgggagat gccgcaacct ggcgatgcat ggtctcctac atctattgcc gaaataatta
      181 aaattgaatc tggagtgcgt gctggaaaat atgatggtaa ataccaattt agtgagccat
      241 tatatagttt aaaagcttat ggacattcat ctgatacaaa taatttaggt atttgggcag
      301 ttactggaaa tcacgaatat tttagtggag gaccaaccag tcatgattta aattcagcat
      361 caggaattat tcatatatta atgaatgggg tacattataa ttcaactgga tttcaagtgc
      421 cgcttggcga agaatggtca aaaatttatg gtccttattt gatttatgct aatcaaaacg
      481 caacagcaac tgccaattgg gatgaagcaa aagcaagagc ggcagttgat aaaacggaat
      541 ggccatttag ttggttaacc aatactcctg attatcccct agctgcggga agaggaaata
      601 tcattggaaa ttttaacatc accgatcctt ctaaaaccaa tgtcaatgga aacgaagctt
      661 ggataggagt tactcaatta agtccagatg ctggtggaaa ttggcaattc gaagaaaaaa
      721 attatcaata ttgggttcaa acagatgctt ctggaaattt tgatattaaa aatgtacgcc
      781 caggaaccta tactttattt gcctacaaaa atggtactac tggagaatat cgacaagaaa
      841 cagttgttgt taccgcagca gcaaccacta atcttggaaa cataaattgg actattcctc
      901 gcaccaatgg aaatttagtt tttgaaattg gagttcctaa tcgaactgcc gaggaataca
      961 aatttggcga tttcgactat tgcgaaggtt ttgtagaaaa caaatttgca gcaactttta
     1021 ctaatccaat cgaatacaat gtcgaagata aaaattggcc cacagcactt tcttatgttc
     1081 attcagccta tttcaatgcc gatgggacaa gatctgtttg ggattggaat gtaaatttta
     1141 atttgactgg gaccatccct accacaggaa atgcaaaatt aacaattgcc tatgccagtt
     1201 cagaccacgc tcaaaattgg atttttatca acggaagtcg cgttacacca tcttctggat
     1261 attatccacc taatggcgga ggaaatgcat ttttaagaca atccaatcac gcaaaatatg
     1321 gattagccac ttttgacatt ccatatagta aattaaaatc gggaaaaaac accctcaaat
     1381 tgcaaatgcc atctacatca agtggaggca atcacgtgat gtatgattat atcagtttag
     1441 aaggggatct aaccaccttg ggcatcaatt caaaaggggt ggacgtagct gatttcagaa
     1501 tgtatcctaa tcctacaaaa ggaatttttg aaattgcctt gccgctttca attcaagaaa
     1561 ccactatcga attatatacg atgaatatgc aattgatttc caaaaaggca tatactccag
     1621 ttaatgggaa agtgcaatta aatatagaaa atcaagctaa cggagtttac tttgcaaaaa
     1681 ttggaactac aggagtgact tttaaagtta taaaagaata ggtcagtggc ttgatgactt
     1741 cttattaaag ttgaataaaa aaggtttgat gaaattcgtt tcatcaatct tttttaatta
     1801 ttcaaataga gtttcatcat tatgtccgtt tgctcgtcat ctcctaattt gaaaatgtgt
     1861 ttgtcaaact ctacaaaacc atttttcttg taaaaacgaa tggctcttgg attttcttcc
     1921 caaaccccca accaaacata atccacattt ttttgttttg ctatttgaat ggctttgtca
     1981 taaagcaatt gtccaacact ttttccgtga aagtctttcg acacatagat tcgctcaatt
     2041 tcaagggctt tgctgtcttt taattcggtt tgcgatgctc caaagttgat ttttaaatag
     2101 ccaattactt cattgtcgag tgttgcaaaa tagaactcag agttttgatc tttaagttct
     2161 aaagtcagtt tttcgttgga aaatccttcc tccaaatagt ttttcatatt ctcttcggaa
     2221 ttggattctg aaaaggtttc ctcaaaagtt tgcctgccaa ttttttgcaa ttggtcaagg
     2281 tggcttagtg ttgcttttgt tatttcaata gttgccattt tctgattcgg tattatttaa
     2341 ttccatttac atttacttct ttagcatctt ttttaaattg attttcaagg gcaattttca
     2401 ttttttcggc taaagtttga ttttgcaaaa tcacattatt tttctcactt ggatcttttt
     2461 cgatattata caattcatag tcagcaggaa tagtcaagtg tcgggttcta attattttcc
     2521 aaggactttg tattaaactt tcctgcaaat gacctctcac ataaatttct tgattgggaa
     2581 tttctaagtc cttatagcta tcgggatcat gttcagaaat aacagaccaa gcattttttc
     2641 cctcaacggt tttaggaagt tttttatctc cagccaaaaa caaaaaggtt ggcaatacgt
     2701 ctataatcga aatgtaacct tcatttttat aatggacaag ctgtcctttc caataaaata
     2761 cgcaaggcac tcgaatcgca ccctcataat tagaagtttt ccaatcccta agcggcgtat
     2821 tgtcacccag tctgtcattc gaaggatgct catcattgta ttcgccttta gaatcccagt
     2881 tttccattgc gccattgtca ctcataaaaa caatcagggt gtttttatct aaattttctt
     2941 gttttagttt atccaataac aagccgatac tgttgtccaa atgactcatt gcggcggcat
     3001 aatctcttcg ggaactattc tttatggaag tcatataagg gtctttccat ttctgttctt
     3061 cctgcaaagg aaaatgcggc gcactataag ccacttgcaa aaagaatttt ttcttcgaat
     3121 ctcttttttc cgaaatccac tgaatggctt cttgggtgat taaatctgtt gcgtgtcctt
     3181 tctctacgat aaactcgcca tttctgtacc aactctcgtc accattttta tatttatggg
     3241 tgtattggtc gatttgtccg tgcaaaaagc cataggaata atcaaatcca taggcctttg
     3301 ggccgctact aggttgcaaa cccaaatgcc attttccaaa aagtgccgtt tcgtaattat
     3361 tttgatgtaa taattggggt agagtaggga tggagtcggg tagttttaag gtactcttgt
     3421 cactaattgg tgccacaatt cccatacgac tggctggttt tcctgtcaat aaactggctc
     3481 tcgatggaga acaagttgga ttggcataaa aacggtttaa gatcacccca ttttttgcca
     3541 aacgatcaat attgggagtt tgtatttccg aaccattata gcccacgtca ttccaaccgg
     3601 catcatcggc aataattaaa ataatattgg gtttttcaat tgatttgact tgcttttttt
     3661 gaccaaatga aaccaaaaag aagaacaata gcgtgaatgt aaaacctatt tttgaattaa
     3721 aattattcat tgtaagg
//
LOCUS       DAOSNN010000251         3680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_25186_length_3680_cov_5.589241, whole genome shotgun sequence.
ACCESSION   DAOSNN010000251 DAOSNN010000000
VERSION     DAOSNN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25186_length_3680_cov_5.589241"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLQ70_10810"
     CDS             <1..641
                     /locus_tag="PLQ70_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQE35149.1"
                     /translation="SEVSALLGRMPSAVGYQPTLATEMGAMQERITSTNKGSITSVQA
                     VYVPADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILAPHIIGDEH
                     YNCAQRVKEILQKYKQLQDIIAILGMEELSEDDKLAVSRARRVQRFLSQPFHVAEQFT
                     GIPGVLVDIKDTIKGFNMIIDGELDHLPEAAFNLKGTIEDAIEAGEKMLAEA"
     gene            719..1000
                     /locus_tag="PLQ70_10815"
     CDS             719..1000
                     /locus_tag="PLQ70_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HQE35150.1"
                     /translation="MILEIVSPEAKLFSGEVTSVTLPGVDGSFQILDNHASIVSILGK
                     GLVKIAAPSFNFSEEVEAKFTKVNPQTYTLAIASGTIEMKDNKVIVLAD"
     gene            1138..2295
                     /locus_tag="PLQ70_10820"
     CDS             1138..2295
                     /locus_tag="PLQ70_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HQE35151.1"
                     /translation="MKFPKNLTTKLEIRKQNNALRKLAAPNNLIDFSSNDYIGFSKNE
                     TIFEQTHQYLLDNNCIQNGATGSRLISGNHKMYQEAENYIAQFHQSESALIFNSGYDA
                     NVGFFSSVPQKGDLILYDELCHASIRDGIIMSNAKSYKFKHNDFEDLERLLLKFLPTP
                     NTQHPAPIYIVTETVFSMDGDCPNMEELVAVSEKHKCYLVVDEAHALGVFGENGEGLV
                     QMLDLQNKVFARIMTFGKGLGCHGAAIVGSQELCDYLVNFARSFIYTTGLSPHSVATI
                     LVAYQNLQSEKETIEKLRENIVHFNQEKNLLGLKPMFVRSKSAIQSAIIPGNENVKSI
                     ACQLQEKGFDVKAILSPTVPVGQERLRFCLHSFNSKEEISGVLSLLSTFVF"
     gene            2301..2708
                     /locus_tag="PLQ70_10825"
     CDS             2301..2708
                     /locus_tag="PLQ70_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HQE35152.1"
                     /translation="MEDIFELIGAYQYSSEAIIYKGKLESEGIAVFMRDNYTVDSNPL
                     YSNAVGGVKLFVNQKDYPKAKDIISQVSQYSLDDDHKLLKCPNCGAEQIDMVTSVKDL
                     KSLIIYIFSLLLGTLPFYSKHKYKCNKCNFEFN"
     gene            2709..3326
                     /gene="bioD"
                     /locus_tag="PLQ70_10830"
     CDS             2709..3326
                     /gene="bioD"
                     /locus_tag="PLQ70_10830"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HQE35153.1"
                     /translation="MKIFVTGISTDVGKTIASSIIVEALQADYWKPIQAGDLENSDSH
                     KIKSYISNTESKFHTNAYALNTPASPHLAAELDGITLDLKNSKEPETKNHLVIEGAGG
                     VLVPLNNNDCVIDLIQKDYKVIVVSRHYLGSINHTLLTIEALKNRQLNIVGIIFSGDE
                     NKSSESIILNKTGVKCIGKIEQEPYFDKNVIKEYADLFRENLLNL"
     gene            3386..>3680
                     /locus_tag="PLQ70_10835"
     CDS             3386..>3680
                     /locus_tag="PLQ70_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015783113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQE35154.1"
                     /translation="MNLTERDQNNIWHPYTQHKTAALPIAIKKGKGALLWDENNKEYI
                     DAIASWWVNPYGHSNTFIAEAIYKQLTTLEHVLFGGFTHEPAIVLAEKLLKILP"
BASE COUNT         1217 a          642 c          693 g         1128 t
ORIGIN      
        1 gttctgaggt atcggcactt ttgggtcgta tgccatctgc tgtaggatat caaccaacat
       61 tggcaacaga gatgggagcc atgcaagagc gtattacttc tacaaacaaa ggatctatta
      121 catctgtaca agcggtttac gttcctgcgg atgacttaac tgacccggca ccagcaacaa
      181 catttgctca ccttgatgca acaactgtat tgtctcgtaa aattgctgag ttaggtattt
      241 atccagcggt agatccattg gattctactt ctagaatatt agccccacac attattggtg
      301 atgagcatta taactgtgca caaagagtaa aagaaattct tcaaaaatac aaacaattgc
      361 aagatattat cgcaattttg ggtatggaag aactttctga agacgataaa ttggcagttt
      421 caagagctag acgtgtacaa cgtttcttgt ctcaaccttt ccacgtagcc gagcaattta
      481 caggtattcc tggagttttg gttgatatta aagataccat caaaggattc aacatgatta
      541 ttgatggaga attagaccac ttgccagaag cagctttcaa ccttaaaggt acaattgaag
      601 acgctatcga agcaggagaa aaaatgttgg cagaagcgta attggttgtt agttgttagt
      661 tgatagttgt tagaaaacaa ccatcaacca tcaaccatca actattaact aaaaaattat
      721 gattttagaa atagtatcac cagaagcaaa attattttca ggagaagtta cttcggtaac
      781 cttacctgga gttgatggta gctttcaaat tttggataat cacgcttcga ttgtttctat
      841 tttaggaaaa ggactagtga aaattgcagc accaagtttt aatttttctg aggaagtaga
      901 ggctaagttt acgaaagtaa atccacaaac ttatactttg gctattgctt cgggaacaat
      961 cgaaatgaaa gacaataaag taattgtttt agcagactaa gacaatttca aggcaaagaa
     1021 ttaaaagtca tacagcaatg tgtgactttt tttatgtcaa aaaatccggg tttatccgtg
     1081 tttttgcttt ggcaaatctg tttcatctgc gttccatttt ttcctaactt tacccttatg
     1141 aagtttccaa aaaatctcac aaccaaactc gaaatccgca agcaaaacaa tgctttgcga
     1201 aaattggctg cgcccaataa tttaatcgat ttttcgtcta atgattacat tggtttttca
     1261 aaaaatgaga ccatttttga gcaaacgcat cagtatttat tggataataa ttgcattcaa
     1321 aacggggcaa caggttctcg attaatatca ggaaaccaca aaatgtatca agaagcggaa
     1381 aattatattg cccaatttca tcagtcggaa tcggctttga ttttcaattc gggttacgat
     1441 gctaatgtgg gtttttttag ttccgttcct caaaaaggcg atttgatttt gtatgatgaa
     1501 ttgtgtcatg cttccattcg ggatgggatt ataatgtcga atgccaaatc ctataaattc
     1561 aaacacaatg attttgagga tttggaacgt ttgcttctga aatttctccc aacacccaac
     1621 acccaacacc cagcacccat atacattgta accgaaaccg ttttttcgat ggatggcgat
     1681 tgccctaata tggaagaatt ggttgccgtt tccgaaaaac acaaatgtta tttggtggtt
     1741 gatgaagcac acgcattggg tgtttttgga gaaaacggtg aaggactagt tcaaatgctg
     1801 gatttgcaaa ataaagtttt tgcccgaata atgactttcg gaaaaggatt gggctgtcac
     1861 ggagcagcca ttgtgggaag tcaggaattg tgtgattatc tagtcaactt tgcacgaagt
     1921 tttatttaca ccacgggact ttctccgcat tcggttgcca caattttagt ggcttatcaa
     1981 aatttacaat ccgaaaaaga aaccattgaa aaacttcgag aaaacatcgt tcatttcaat
     2041 caagaaaaaa acctattagg attgaaaccg atgtttgtta gaagtaaatc agccattcaa
     2101 agtgccatta ttcctggaaa cgaaaacgta aaatcgatag cctgtcaact tcaggaaaaa
     2161 ggatttgacg tgaaagccat tttgtcgcca acggttccgg taggacagga acgattacgg
     2221 ttttgtttgc atagtttcaa ttcgaaagaa gaaatctctg gagtcttaag tttgttgagt
     2281 acttttgttt tttaaaattt atggaagata tttttgaatt aataggggca tatcaatatt
     2341 catccgaagc aataatttat aaaggaaaat tagagtcgga aggcattgcc gtttttatgc
     2401 gggataatta tactgtcgat tccaatccat tatacagtaa tgccgtcggt ggagtaaaac
     2461 tttttgttaa ccaaaaggat tatccgaaag cgaaagacat tatttctcaa gtgagtcaat
     2521 attcattaga tgatgatcat aaattactga aatgtccaaa ttgtggggca gagcaaatag
     2581 atatggtcac ttccgtaaaa gatttgaaat cgcttatcat ttacattttt tcattgcttt
     2641 taggaacatt gcctttttat tccaagcata aatacaaatg caataagtgt aattttgaat
     2701 tcaattaaat gaaaatattt gttacaggaa tctctactga tgttggcaaa accattgcct
     2761 catccatcat tgtcgaagca ctccaagccg attattggaa acccattcaa gctggtgatt
     2821 tggagaattc cgacagtcat aaaatcaaaa gctatatttc aaatacggag tcaaaattcc
     2881 atactaatgc ttatgcgctc aacacgccag ccagtccaca tttggcagct gaattagacg
     2941 gaattactct cgatttaaaa aatagtaaag aacccgaaac caaaaatcat ttggttatag
     3001 aaggagctgg cggtgttctc gttccattaa acaacaatga ttgcgttatc gatttaatcc
     3061 aaaaagatta taaagttatt gttgtttctc gccattattt gggaagtatc aatcatactt
     3121 tattgacgat tgaggcttta aaaaacagac aactaaacat cgttggaatc attttcagtg
     3181 gtgatgaaaa taaatcttcc gaatcgataa ttttgaataa aactggagtg aaatgtattg
     3241 gaaaaatcga acaagaacct tattttgata aaaatgtcat caaagaatac gccgatttgt
     3301 ttcgagagaa tttattaaat ctttaaaatt tctttgtata tctctgtgtt ttcttcgtgt
     3361 atctttgtga aataaatttt tcgaaatgaa cttaaccgaa agagaccaaa acaatatttg
     3421 gcatccttat acccagcata aaaccgcagc gcttcccata gcgataaaaa aaggaaaagg
     3481 cgcactactt tgggacgaaa acaacaaaga atatatcgac gccattgctt cttggtgggt
     3541 aaacccttac ggacattcca atacatttat tgctgaagca atttacaagc aattgaccac
     3601 tttggagcac gttctttttg gcggattcac tcacgaacca gcgatagttt tggctgaaaa
     3661 actgttgaaa attttgccga
//
LOCUS       DAOSNN010000252         3652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_25424_length_3652_cov_3.886850, whole genome shotgun sequence.
ACCESSION   DAOSNN010000252 DAOSNN010000000
VERSION     DAOSNN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25424_length_3652_cov_3.886850"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PLQ70_10840"
     CDS             <1..1252
                     /locus_tag="PLQ70_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35155.1"
                     /translation="AEVTLFNENLEPLAERLVYVNQDQKLLIKTELNQSGFSTRDKAN
                     LKIKVTDKDGKPIVAHLGVSVYDALYQNKQDSKNIQSHYLLSTQLKGNIYDPAYYFNE
                     KNKDSKQALNLLLLTQGWRNYVWNESNLKEQVANHPIVFDEWKGSVRLKKIKKKDTEP
                     IGKKTLTVVTANEKRGKYFIATDSIGGFNVGPKELKRGEAGYAYLELLSNPDSKYRTH
                     FTDTFFEGINQNRKSKNLIYPISAPQEIKPEAASPFVGRATITKLKEVIITSKKTKGF
                     RDKYIGKLDSLARLDRISDYYCINGILNCKNHPQARFEYRDLTEAELLEMFNILMIEG
                     YYGKKEFYEAVYDEVTLMDSTPDFRNTLFWKSDVITNKNGEASLDFFCSDINSLFIGN
                     VEGVSSDGLLGADNFEFKVRKKTN"
     gene            1475..3181
                     /locus_tag="PLQ70_10845"
     CDS             1475..3181
                     /locus_tag="PLQ70_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HQE35156.1"
                     /translation="MKRLKNALLLSITILLALIFSSKTEAQTSLQKQSASSAFVFSKG
                     ADVGWLSQMEATGYVFYDTDGTPKDCLQLLKDRGMNTIRLRVWVNPSNDKTNGHCSKA
                     ETVAMAVRAQNLGMRVMIDFHYSDSWADPAKQTKPAAWATHTFAQLLDDVYNHTFDVL
                     TDLKTAGVTPEWVQIGNEIPGGMLWPEGSNSTSFSQLSQLLNKGYDATKAINPAIKVV
                     VHIDKGNDNARFRWFFDNITANSVKYDVIGMSYYPYWLGSDYSATIAALGTNLKDMAS
                     RYGKEVMVVEVGGDYTLVQNTKDMLIAVISAVKSVPNEKGLGVIYWEPEGEKTWSGYQ
                     LNAWQSNGRPSPALDAFKDGAVSEAEQVTINGFVANPVEQGKTVTTNLTYTANTATDY
                     FYVGLFKRNSSGAWMQTIAESTGNQFLIPSTGVNVPSQVTLSIPLATTPSTSLANGEY
                     YDVLVELWTAGWGLKLGSSLSPKLTIAASGSLGVEAKKYDTELVVYPNPVSDTLNIKI
                     PNGKIIKSFKIANILGETIYVKENISNQNAIDVSNLSSGVYILSVNFGNYTNEHIKFQ
                     KK"
     gene            3254..>3652
                     /locus_tag="PLQ70_10850"
     CDS             3254..>3652
                     /locus_tag="PLQ70_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQE35157.1"
                     /translation="MILNIKPIFIFCAILCLFSCKSQTVVASKKAEIVSDTTFVNLKD
                     YSQDFVYDMKYATSDNFLKAKVYDCAECFLRLKTIKALISANADFIKMGYKIKIFDCY
                     RPLDIQKRMWKIVPNPSYVADPSKGSIHNRG"
BASE COUNT         1256 a          589 c          723 g         1084 t
ORIGIN      
        1 tgccgaagtc actcttttta atgaaaattt agaaccattg gcagagcgat tggtatatgt
       61 aaatcaagat caaaaattac taattaagac agaactcaac cagagtggtt tttccacccg
      121 agacaaagca aatctcaaaa taaaggttac tgacaaagac ggaaaaccca tagtagccca
      181 tttgggagta agcgtttacg atgccttgta tcaaaacaaa caagattcca agaacattca
      241 aagtcattat ttgctttcta ctcaattgaa aggaaatatt tatgatccag cttattattt
      301 taacgagaaa aacaaagaca gtaaacaagc cttgaattta ttgttgctca cccaaggttg
      361 gagaaattat gtttggaatg aaagcaatct aaaagaacaa gtagccaatc atcccattgt
      421 ttttgatgag tggaagggaa gtgttcgctt aaaaaaaatc aagaagaaag atactgaacc
      481 cattggaaag aaaacattaa ctgtggtcac agccaatgag aagaggggca agtattttat
      541 agcgactgat tcaattggag ggtttaatgt gggaccaaaa gaattaaaaa gaggagaagc
      601 tggttatgct tatttagaat tgttgtccaa tcctgattct aaataccgta cccattttac
      661 ggatactttt tttgaaggaa taaaccaaaa tcgcaaaagt aaaaacctga tttatcccat
      721 ttcggcacca caagaaataa aaccagaagc agcttctcct tttgttggcc gtgccaccat
      781 aaccaaatta aaggaagtta taattacatc aaaaaagacg aagggttttc gtgataaata
      841 catcggaaaa ctagacagtt tggcgagatt ggatcgaatt agtgattatt attgtattaa
      901 tggaattctt aactgtaaaa accacccaca agctagattt gaataccgag acctgactga
      961 agccgagttg ttggaaatgt ttaatatcct aatgatcgaa ggctattatg gcaagaaaga
     1021 attttacgaa gccgtctatg atgaagtaac actaatggat tcaaccccag atttccgcaa
     1081 tactttattt tggaaatcgg atgttattac gaataaaaat ggcgaagcta gcctggattt
     1141 tttctgttcc gatatcaatt ctctttttat tggaaacgtc gaaggagtca gtagcgatgg
     1201 attattggga gcggataatt ttgaatttaa ggttagaaaa aaaacaaatt aaattaggta
     1261 attaattgta aatgagttag atggttgttt atttggtgct atgcgaaaat aacttcataa
     1321 ggttagctta catttaggga ctaatgatta tttcgacgaa tagattatca aaaaatagtt
     1381 tgtaaatttg ggaattaaaa tagtttctta gaaaagaaaa atcaatgatt tgaaatagtc
     1441 ttgactataa ataatttata aatagagtac cattatgaaa agattaaaaa acgcattgtt
     1501 attgtcaata accattttat tggcattgat tttttcttcg aaaactgaag cgcaaacttc
     1561 tctacaaaaa caatcggctt catcagcttt tgtcttttca aaaggagccg atgtgggttg
     1621 gttgtcacaa atggaagcca caggctatgt tttttatgat accgatggaa ctccaaaaga
     1681 ttgtttgcaa ttgctaaaag accgtggaat gaacaccatt cgtcttcgag tttgggtcaa
     1741 tccttcgaac gataaaacca atggacattg cagcaaagcc gaaacggttg cgatggccgt
     1801 tcgtgctcaa aatttgggaa tgcgagtgat gatcgatttt cattatagcg attcttgggc
     1861 cgatcctgcc aaacaaacca aacccgcagc ttgggcaacc catacttttg cacaattatt
     1921 agacgatgtt tacaatcata cttttgatgt tttgactgac cttaaaacgg caggcgtaac
     1981 tccagaatgg gttcaaatag gcaatgaaat tccaggcgga atgttatggc ctgagggaag
     2041 taattcgaca agttttagtc aactttcaca acttttgaat aaaggttatg acgcaacaaa
     2101 agcaataaat ccagccatca aagtggtggt tcatattgac aaaggaaatg acaatgccag
     2161 attcagatgg ttttttgata atattactgc caatagcgta aaatatgatg taatcgggat
     2221 gtcttattat ccatattggc tgggaagcga ttattcagcg accattgccg ccttgggaac
     2281 taatctgaaa gatatggcaa gccgatacgg aaaagaagta atggtagtag aagttggagg
     2341 cgattacacc ttggttcaaa acactaaaga tatgttgatt gcagtgattt cggctgtgaa
     2401 aagtgtgcca aacgaaaaag gccttggcgt gatttattgg gaaccagaag gcgaaaaaac
     2461 ttggagcggc tatcaattga atgcttggca atccaatgga agaccttcgc ctgctttaga
     2521 cgcttttaaa gacggtgccg tttctgaagc ggaacaagtt accataaacg gatttgttgc
     2581 caatcctgtg gaacaaggca aaacggtaac tacaaatttg acctatacag ccaacaccgc
     2641 aaccgattat ttttatgttg gtttatttaa aaggaattcg agtggagctt ggatgcaaac
     2701 tattgccgaa agtacaggaa atcaattttt aattccatca acaggtgtta atgtgcctag
     2761 tcaagttacg cttagcatac ctttagccac aacaccaagt accagtttag ccaatggcga
     2821 gtattatgat gttttggtag aactttggac agcgggttgg ggattgaaat tgggttcaag
     2881 tctttctcct aaattgacca ttgcagcttc aggttcatta ggggttgagg ctaaaaaata
     2941 tgataccgaa ttagtggtct atcccaatcc tgtttcggat actttgaata ttaaaattcc
     3001 taatggaaag atcattaaat cattcaaaat tgccaatatt ctgggtgaaa ctatttatgt
     3061 taaggagaat atttcgaatc aaaacgccat tgatgtgtcg aatttaagtt caggtgttta
     3121 cattttatcg gttaattttg gtaattatac caatgaacat attaaatttc aaaaaaagta
     3181 aattgcaact agattttaag gagtatttac tcaatttgaa aatgtatcaa tcgatttaag
     3241 ctcaataaaa gccatgattt tgaatatcaa acctatcttt attttttgtg caattctgtg
     3301 tctgttttcg tgtaaatcac aaactgttgt tgcgtccaaa aaagccgaaa tagtgagtga
     3361 tacaacattc gtaaatttaa aagactacag tcaagatttt gtttatgata tgaaatacgc
     3421 cacttcagac aattttttga aagctaaagt atatgattgc gccgaatgtt ttttgcgatt
     3481 aaaaaccatt aaggcattga ttagtgccaa tgccgatttt ataaaaatgg ggtacaaaat
     3541 caaaatattc gattgttacc gccctttaga cattcaaaaa aggatgtgga aaattgtacc
     3601 aaacccttcc tatgttgcag atccaagcaa aggttcgatt cacaatagag gt
//
LOCUS       DAOSNN010000253         3651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_25433_length_3651_cov_6.650723, whole genome shotgun sequence.
ACCESSION   DAOSNN010000253 DAOSNN010000000
VERSION     DAOSNN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25433_length_3651_cov_6.650723"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2120
                     /gene="recG"
                     /locus_tag="PLQ70_10855"
     CDS             <1..2120
                     /gene="recG"
                     /locus_tag="PLQ70_10855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQE35158.1"
                     /translation="ETPIEYLKGVGPNRGTLLRKELGIHKYGHLLNFFPNRYIDRTRY
                     YKINELQNNIAEVQIIGKIINVKTVEFGKNQKRLVATFVDDTGQMELVWFQGHKWIRE
                     SLKLNEICVIFGKCTSFGNTFNMAHPEIELMTEHQQSLRSAMQPVYPSTETLANRGIS
                     NRVINKMMQQLFVETQAKFSETLPDYLIAELKLIPKNAALFNIHFPKSAEALAKAQFR
                     LKFEELFYIQLQLITKNLIRKHKIKGHPFTKVGDYFNNFYQNHLPFELTNAQKRVIKE
                     IRIDMGSNAQMNRLLQGDVGSGKTIVALMSMLIALDNGFQSCLMAPTEILANQHFNGL
                     SELAKELNINIKILTGSTKTAARKIIHEELENGSLHILIGTHALLEDKVKFQNLGLAV
                     IDEQHRFGVEQRSKLWKKSNPNPSDEGEHIPPHVLVMTATPIPRTLAMSLYGDLDISV
                     IDELPPGRKPIQTVHRFDSNRLKVWKFIRDEIAIGRQIYIVYPLIEESKTMDLKDLMD
                     GYESISRDFPLPQYAISILHGKMKPADKDAEMKRFAEGKTNIMVATTVIEVGVNIPNA
                     SVMIIESAERFGLSQLHQLRGRVGRGADQSYCILMTSHKLSADSKTRMETMVKTTDGF
                     EIAEVDLKLRGPGDLMGTQQSGVLNLQIADIVRDRDILQLARNYALKILKDDAPMQKP
                     ENAVLRATYIELTKKKNIWNYIS"
     gene            2373..3434
                     /locus_tag="PLQ70_10860"
     CDS             2373..3434
                     /locus_tag="PLQ70_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQE35159.1"
                     /translation="MKIKHIVYTLLVIGFGAFIAYRIVSNKNKNGDSKGKDGKNKEIT
                     VNGIVIKTETFDNTISLSGSIEANEQVEIRSEISGIVEGIYFKEGSNVSKGQLLFKVN
                     DVELRAQLQQAKTKQGLASENERRAKLLLAKEAISQEEYDVARADFKLAQSQVQLIDA
                     QIAKTSVRAPFSGKIGLRSISPGTYITPSLLVAKLVNVGQLKITFSVPEKYANQIKVN
                     SNLSFTVVGTSEKHFAKVYAIEPEIEITTRTLQIRALAENKDGKLLPGTFANVELPLD
                     VIKDAVVIPTEAIVPVQNGKKVFISNNGKATEIMVETTTRTDASVLVLSGLKVGDTLI
                     TSGVMSLKKESPVKIKIKG"
BASE COUNT         1235 a          703 c          697 g         1016 t
ORIGIN      
        1 tagaaactcc catagaatac ctcaaaggcg tcggtcccaa ccgcgggact ttgctgcgca
       61 aggaattggg aattcacaag tacggtcatt tgctcaattt ttttcccaat cgttacattg
      121 atagaacacg ctattataag atcaacgaat tacaaaacaa tattgccgaa gtgcaaatca
      181 ttggcaaaat catcaatgta aaaacggttg aatttggtaa aaatcagaaa cgattggtcg
      241 ctactttcgt ggatgatacg ggacaaatgg aactcgtttg gtttcaagga cacaaatgga
      301 ttcgcgaaag tttgaagctc aacgaaatct gcgtaatttt cggaaaatgt acttcgtttg
      361 gaaacacttt caatatggca catcccgaaa ttgagttgat gacggagcac caacaaagtc
      421 tgcgctcggc gatgcaaccc gtttatccat cgaccgaaac tttggcaaac cgaggcattt
      481 cgaatcgggt catcaataaa atgatgcagc aattgttcgt ggaaacccaa gccaaattca
      541 gcgaaacttt gcccgattat ttgattgccg aattgaaact gattccaaaa aatgcggctt
      601 tgttcaatat tcattttcca aaaagtgccg aagctttggc gaaagcccaa ttccgattaa
      661 aattcgagga attgttttat attcaattac aattaattac caaaaacctg attcggaagc
      721 ataaaatcaa aggacatccg tttacgaaag tgggcgacta tttcaataat ttttaccaaa
      781 atcacctgcc ttttgaactg accaatgccc aaaaaagagt catcaaagaa atccgtattg
      841 atatgggcag caacgcccaa atgaaccgat tgctgcaagg cgacgtgggt tcaggaaaaa
      901 caattgtggc tttgatgagt atgctcattg ccttggacaa cggttttcaa tcctgcttga
      961 tggcaccaac ggaaatcttg gccaaccaac atttcaacgg cttgtcagaa ttagccaaag
     1021 aactgaatat caacataaaa atactgacag gttccaccaa aactgcagct cgaaaaatca
     1081 ttcacgaaga acttgaaaac ggcagcttgc acattctcat tggtacacac gctttattgg
     1141 aagacaaagt aaaatttcaa aatttgggct tggccgtgat tgacgagcaa catcgttttg
     1201 gggtagaaca acgatcaaaa ttatggaaga aatccaatcc taacccctct gatgaaggag
     1261 aacatattcc gccacacgtt ttggtaatga ccgccacgcc tattccgcgg actttagcga
     1321 tgagtttgta tggtgatttg gatatttcag tgattgacga attgcctccg ggacgaaaac
     1381 ccattcaaac tgtgcatcga tttgatagca atcgactgaa agtatggaaa tttattcgag
     1441 acgaaattgc cattggcaga caaatttata tcgtctatcc attgattgaa gaatcgaaaa
     1501 caatggattt aaaggatttg atggacggtt acgaaagcat ttcccgtgat tttccgttgc
     1561 cgcaatacgc tatttccatc cttcacggaa aaatgaaacc cgccgacaaa gatgccgaaa
     1621 tgaaacgctt tgccgaagga aaaaccaata ttatggtcgc tacaaccgtg attgaggttg
     1681 gcgtgaatat tcccaacgct agcgtaatga tcatagaaag tgccgaacgt tttggactgt
     1741 ctcaactcca ccaacttcga ggtcgcgtgg gtcgtggtgc tgaccaaagt tattgcatcc
     1801 tgatgacgag tcacaaattg agtgccgata gcaaaacccg aatggaaacg atggtaaaaa
     1861 caactgacgg tttcgaaatt gccgaagtcg atttgaaatt gcgtggtccc ggagatttga
     1921 tgggaaccca acaaagtggc gtactaaacc ttcaaattgc cgatattgtt cgtgatagag
     1981 atattttaca attggcacgc aattatgcct tgaaaatcct aaaagacgat gccccgatgc
     2041 aaaaaccgga aaatgccgtt ttgagagcca cttacatcga gttgaccaag aagaaaaaca
     2101 tttggaatta tattagttaa agtgagctga atagcacaca gataaaacgg atttacttcc
     2161 taaaaacact gataaaaaat cgattttttt taaattaaat taaaccattg tatttttttc
     2221 cgtgtctaac taaattgtca tttttagagt attaaaattc attgttaatc agttgttaaa
     2281 taattgacaa aagccccatt tttcaaaatt ctaaaaccta aatttgtcat tatatacaaa
     2341 acgagaaatc taatctcact ttctggacta ttatgaaaat aaaacatatc gtttacacct
     2401 tgctagttat tggtttcgga gcatttattg catatcgaat tgtttcaaat aaaaataaaa
     2461 atggcgattc taaaggaaag gatggcaaaa acaaagaaat aaccgttaat ggaattgtca
     2521 taaaaacaga aactttcgac aatactattt cgctttctgg ttctattgaa gccaatgaac
     2581 aagtagaaat tcgctctgaa atttctggaa ttgtagaagg aatttatttt aaagaaggca
     2641 gcaatgtttc aaaaggacaa ttgcttttta aagtaaatga tgtcgaattg agggcgcaat
     2701 tgcaacaagc aaaaaccaaa caaggattgg cttctgaaaa cgaaagaaga gccaaattat
     2761 tactcgcaaa agaagccatc agccaagaag aatatgatgt ggccagagcc gatttcaaat
     2821 tggcacaatc tcaagtacaa cttattgatg cccaaattgc caaaacttcg gttagagcgc
     2881 ccttttctgg aaaaattggt ttgcgttcca tttcacccgg aacctacatc actcctagcc
     2941 ttttggttgc caaattggtg aacgttggtc aattaaaaat cactttttca gtacctgaaa
     3001 aatatgccaa ccaaataaaa gtcaattcga atttgagttt taccgttgtg gggacttctg
     3061 aaaaacattt tgccaaagtg tatgccatcg aacccgaaat tgaaatcact acccgaactt
     3121 tacaaatcag agctttggca gaaaacaaag atggaaaatt attgcctgga acttttgcca
     3181 acgtcgaatt gccattggat gttataaaag atgccgtcgt gattccaaca gaagccatcg
     3241 ttcctgttca aaacggaaaa aaagttttta tatccaataa cggaaaagcg accgaaatta
     3301 tggtagaaac aactacaaga actgacgcat ctgttttagt cctttctggg cttaaagttg
     3361 gagacaccct gattacaagt ggtgtaatgt ctttgaaaaa agaatctcca gttaaaataa
     3421 aaatcaaagg ctaagattta ttaggttttg agttatggat tttaattatc tattctcttc
     3481 attctaaaat ctaatgtcta aaatctaaag tcacaatgag tttatcaacc accagcataa
     3541 gaagacccgt ttttacaatc gttatcaatc tagccatagt cttgtttgga attattggtt
     3601 acacttttct tggcgttcga gaatttcctt ccattgatcc tgctcaagtt t
//
LOCUS       DAOSNN010000254         3642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_25524_length_3642_cov_4.596878, whole genome shotgun sequence.
ACCESSION   DAOSNN010000254 DAOSNN010000000
VERSION     DAOSNN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25524_length_3642_cov_4.596878"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /locus_tag="PLQ70_10865"
     CDS             complement(<1..1791)
                     /locus_tag="PLQ70_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HQE35160.1"
                     /translation="MKKTFLHTFILIASLCFSSAQAQNKISVTNLQCEMLNNPEGIDV
                     VQPRLSWQIKSDVNDVKQTAYQIMVASTLENLNANKADLWDSGKVTSNESVNIIYSGK
                     KLGDRQNAFWKVTVFSNKGEIKSTSTAHFSIGILTYADWKSTRWIGYEKLAKDDSISQ
                     FSRLSARYLRKGIDLKKPVKSAKVYIMGMGLYELYINGNKIGDQVLAPVPTDYTKNVK
                     YNVFDVTSQLKEGKNMLGTILGNGRFFAMRQDYKPYKIKSFGFPKMALQLFVEYTDGS
                     NEVIRTDDTWKLTTDGPILSNNEYDGEEYDARKEMKGWNTTNFDDKNWVNARYVQEPG
                     GFFEAQMSPNMKIMGEVKPISIKATPKGTYILDMGQNMVGWLQLKVKGKSGDQITMKF
                     AESLQKDGSLYIANLRDAKTTDVYTLKGEGEEIWEPRFIFHGFRFVEISGFPTKPTLD
                     NFVGKVVYDDIKTTGTFESSDATMNQIFKNAWWGISGNYKGMPIDCPQRNERQPWLGD
                     RTTGAYGESFLFDNQTLYAKWLDDIKYSQTQDGGLPDVAPAFWRYYGDNVTWPGTYIT
                     VADMLYTQFADKEVIIKQYPTMKKWMVYMEE"
     gene            complement(1856..3100)
                     /locus_tag="PLQ70_10870"
     CDS             complement(1856..3100)
                     /locus_tag="PLQ70_10870"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HQE35161.1"
                     /translation="MRTISNLDLKLQLTNEYLRNGGVQKILDPDLLQDIIDFNIHKPE
                     TITSRLNAFMLVIMGSQMQPPFFSEDFISEYSSFLQKSYCFDQYKIDTIEEFDKIYDE
                     YKNTSEILYRGQSEAKWRLYSNLQRTWILNKLHKKENSYEGFLQKLVLTGQEDYEDKI
                     NEVLKEHNIDTINSISVLGFLQHHGCPTPLLDWTYKFQNALFFAMDGLTRNNVPREIE
                     DYFSVYHIKQSDMEGGGMRQVMDESLDDFDGQYKSELISTFAIDEEQRIEMEKHFQGR
                     KSIDIDRIFGSGIIEYITKIENMMNFNLSFFSDKDSKKGFIFSLNNSHNILNQKGVFT
                     WNSSSMKPLEVVGNEQYFEDVVDGKADDYRFCNCLNINKNLVGHISKRLLQDGITRDF
                     IYPTKDIDTRSLFDKISTKANS"
     gene            complement(3140..>3642)
                     /locus_tag="PLQ70_10875"
     CDS             complement(3140..>3642)
                     /locus_tag="PLQ70_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQE35162.1"
                     /translation="TCEWDGAIWPFASSQTLTALANVLNNYNQTFVAKTDYFKQMDLY
                     VQSQYYRGKPYIGEYLDETTGYWLMGDKERSRYYNHSTFNDLMITGLVGLRPRADEKI
                     EVNPLIPQEKWDWFCLDNVLYHGNIITILWDKTGEKYHKGKGFRIFKNGKEIGVSEKL
                     EKLVSE"
BASE COUNT         1052 a          762 c          531 g         1297 t
ORIGIN      
        1 ttcttccata tacaccatcc attttttcat agttggatac tgttttataa tcacttcctt
       61 gtcggcaaat tgcgtgtaca acatatcggc aacggtaata tacgttcccg gccaagtcac
      121 attatcgcca taataacgcc aaaaagccgg agccacatcg ggcaatcctc catcctgggt
      181 ttgggaatat ttaatatcat ccagccattt ggcatacaaa gtctggttgt caaacaaaaa
      241 actttcacca taagcaccag tagttctatc gcccaaccaa ggctgacgct cgttacgttg
      301 cggacaatca attggcattc ctttgtaatt tccgctgatt ccccaccacg cattcttgaa
      361 aatctggttc atcgtggcat ccgaagattc gaaagttccg gtcgttttaa tatcgtcata
      421 caccactttt ccaacgaaat tgtccaaagt aggtttggtc ggaaaacctg aaatttctac
      481 aaatcggaat ccgtgaaaaa taaaacgagg ttcccaaatt tcttcgcctt cgcctttcaa
      541 cgtataaaca tcggtggttt tggcatcacg caaattggca atgtataatg aaccatcttt
      601 ttgcaaagac tcagcaaatt tcatcgtgat ttggtcgcca gattttcctt ttactttcag
      661 ttgcaaccag cccaccatat tctgacccat atccaaaatg taagttcctt ttggagtcgc
      721 tttaatcgaa attggtttta cttcgcccat aattttcata ttcggcgaca tttgcgcttc
      781 aaaaaaaccg cccggttcct gaacgtatct ggcatttacc cagtttttat cgtcaaaatt
      841 ggtcgtgttc caacctttca tttccttgcg ggcatcatat tcttcgccgt cgtattcgtt
      901 gttcgataaa atcggtccgt cggtggtcag tttccaagta tcatcggttc gaattacctc
      961 attgcttccg tcggtgtatt cgacaaacaa ttgcaaagcc atttttggga aaccaaatga
     1021 tttaatttta taaggtttgt aatcctgacg cattgcaaaa aatcgtccgt ttcctaaaat
     1081 cgttcccaac atatttttac cttctttcaa ttgcgaagtc acatcaaaaa cattgtattt
     1141 tacgtttttg gtgtaatctg ttggaacagg tgccaaaact tggtcgccaa ttttatttcc
     1201 gttgatgtac aactcataca agcccatccc cataatataa actttggcac ttttgacagg
     1261 ctttttcaaa tcaatccctt ttcgcaaata acgcgctgac aaccttgaaa attgagaaat
     1321 actatcgtct tttgctaact tttcatagcc aatccaacgt gtcgatttcc agtcggcata
     1381 agttaaaatc ccgatgctga aatgcgcagt tgaagtcgat tttatttctc ctttattgct
     1441 aaaaacagtt actttccaaa acgcattttg tcggtctccc aacttttttc cgctgtagat
     1501 aatattgaca gattcgttgc tggttacttt tccgctatcc cataaatcgg ctttgttcgc
     1561 attcagattt tccaaagtcg aagcgaccat aatttgatat gccgtttgtt ttacatcatt
     1621 cacatccgac tttatctgcc agctcaaacg gggttgcacc acatcaattc cttccggatt
     1681 gttcagcatt tcgcattgca gattggttac gctgattttg ttttgggctt gcgccgaaga
     1741 aaaacagagc gatgcaataa ggatgaatgt atgtagaaat gtttttttca taagttttat
     1801 ttttttgcca cagatttgaa gaatccgtac gcctattttt agtgttctag ttcaattaac
     1861 tattggcttt cgtagatatt ttatcaaaca agctccgtgt atcgatatct ttagttggat
     1921 aaataaaatc tcttgtgatt ccatcttgca ataatcgttt cgaaatgtgc ccaactaaat
     1981 ttttatttat atttaaacaa ttacaaaatc tataatcatc tgcttttccg tccactacat
     2041 cttcaaaata ttgttcattt cctacaactt ctaaaggttt cattgaactt gaattccaag
     2101 tgaatactcc cttctgattt aaaatattat gactattgtt taaagaaaat ataaaacctt
     2161 tttttgaatc tttatcacta aaaaaactca aattaaagtt catcatattt tcaatttttg
     2221 tgatatattc aataatacct gaaccaaaga ttctatctat atcaatactt tttcttcctt
     2281 gaaaatgttt ttccatttca attcgttgtt cttcatctat agcaaatgtg gatattaatt
     2341 cagatttata ctgcccatcg aaatcatcta aactttcgtc cataacttga cgcattcctc
     2401 caccttccat atcactttgc tttatatggt aaacactaaa ataatcttct atttctcttg
     2461 gaacattatt ccttgtcaat ccatccatag caaagaaaag cgcattttga aatttgtatg
     2521 tccaatctaa caacggtgtt ggacaaccgt gatgttgaag aaagcctaaa acagatattg
     2581 aatttattgt gtcaatatta tgttctttta agacttcatt tattttatct tcataatctt
     2641 cttgtcctgt taagactagt ttttgtaaaa acccttcata agaattttct tttttatgca
     2701 atttgtttaa aatccaggtt cgttgtaaat tactatataa tctccatttc gcttcgcttt
     2761 gtcctctata aagaatttcg gaagtgtttt tatattcatc ataaatttta tcaaattctt
     2821 cgatagtatc aattttatat tgatcgaagc aatatgattt ttgtaaaaac gaactgtatt
     2881 cagatataaa atcttcagaa aaaaatggcg gttgcatttg tgaccccata attacaagca
     2941 taaatgcatt taatcttgaa gtaattgttt ctggtttgtg aatattgaaa tcaataatat
     3001 cttgaagcaa atctgggtct aatatttttt ggactccacc atttcgaaga tattcattcg
     3061 ttaattgcaa ctttaagtca agatttgaaa tggttctcat tttattattt tttaagcata
     3121 taattaataa atcgattttc tattccgata ctaatttctc caatttttct gaaaccccaa
     3181 tttcttttcc gtttttaaag attctaaacc cttttccttt atgatacttc tctcctgttt
     3241 tatcccaaag aatcgtgata atattcccgt gatacaacac attatccaga caaaaccaat
     3301 cccatttttc ctgtggaatc aacggattta cttcaatttt ttcatccgct cttgggcgta
     3361 aacccaccaa tcctgtaatc atcaaatcat taaaagtcga atgattgtaa taacggcttc
     3421 tttctttgtc gcccattagc caataaccgg tcgtttcgtc cagatattct ccaatgtatg
     3481 gttttcctcg gtaatattgc gactgcacat acaaatccat ttgcttgaaa taatcggttt
     3541 tggcaacgaa agtttgattg taattattca aaacattcgc caaagctgtc aaagtctgcg
     3601 aactcgcaaa aggccaaatc gcaccgtccc attcacaagt tc
//
LOCUS       DAOSNN010000255         3585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_26090_length_3585_cov_3.994051, whole genome shotgun sequence.
ACCESSION   DAOSNN010000255 DAOSNN010000000
VERSION     DAOSNN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26090_length_3585_cov_3.994051"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1519)
                     /locus_tag="PLQ70_10880"
     CDS             complement(59..1519)
                     /locus_tag="PLQ70_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HQE35163.1"
                     /translation="MGKVTGFKEFERIDESYIAVEERVVNYNEFTVALSEADITKQGS
                     RCMDCGIPFCHSGCPLGNLIPDFNHMVHQGEWQKASWILHSTNNFPEFTGRLCPAPCE
                     KACVLGLIEDPVSIENLEKNIVERAFKEGWIKPQPPKTRTGKTVAIVGSGPAGLAAAQ
                     QLNRAGHLVTVFERDDAIGGLLRYGIPNFKLEKGIIDRRIAVLEAEGISFKVNTHVGV
                     NYDINDLKAFDAIVLCGGATERRGLPTPGADADGVVQAMDFLTQQTKVVFGQKVENQV
                     LATGKDVIVIGGGDTGSDCVGTSNRHGAKSVTNFEIMPKPPVGRSESTPWPYWPLQLK
                     TSTSHKEGCERNWLINTKEFIKDDKGKLIALKTVNVEWKMVPGQRPELIEVAGSEKTW
                     PCDLALLALGFTGPEKTLSSQLGIELDFRSNYKAEFGKFQTNIPNIFTAGDMRRGQSL
                     IVWAISEGREAARQVDIYLMGSSDLPTKEGGDLPGV"
     gene            complement(1524..>3585)
                     /locus_tag="PLQ70_10885"
     CDS             complement(1524..>3585)
                     /locus_tag="PLQ70_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HQE35164.1"
                     /translation="QQAVRLSDQSSYDAYSKAVNDQAHNLMTIRGLFEFDNLNPISID
                     EVEPWTDIVKRFKTGAMSYGSISREAHENLAIAMNRIGGKSNSGEGGENRKRFQPDMN
                     GDSRNSAIKQVASGRFGVTSHYLSSAKEIQIKMAQGAKPGEGGQLPGEKVLPWIAEAR
                     NSTPYVGLISPPPHHDIYSIEDLAQLIFDLKNANREARINVKLVSEVGVGTIAAGVAK
                     AKADVVLIAGYDGGTGASPLTSLKHAGLPWELGLAEAQQTLVLNNLRSRIVVECDGQL
                     KTGRDVAIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIATQDKELRKNFKGT
                     PEHVINFFYYVAEELRGIMAQLGFRTLAEMVGQTHKINTNKAITHYKAKGLDLSAILH
                     TPDGYSERIVRNTEKQDHALDNVLDFQILKDSHRAMYRKEKMTLNYPIKNTDRSVGAI
                     VSNEISKIYGYLGLPEDTLNINFTGSAGQSLGAFSAHGLTFTVEGNTNDYLGKGLSGA
                     KLIIKKPAKATFVAENNIIVGNVCLFGAIEGEAYINGIAGERFAVRNSGATAVVEGVG
                     DHGCEYMTGGKVVVLGRTGRNFAAGMSGGVAYIYDPENKFKNGLCNTESIAFETIEAE
                     EAEELKALIAKHVAYTNSTRGAELLADWANSLSKFVKVMPTEYKKALKRLETEEQIFE
                     ELTA"
BASE COUNT          987 a          765 c          613 g         1220 t
ORIGIN      
        1 ccccaaaaaa aaagaccttg aaattacatc aaggtcttta tattatttct gtttcagatt
       61 atacgccagg caaatctcca ccttctttag taggtaaatc actagaaccc attagataaa
      121 tatctacttg tctagcagct tctctacctt ctgaaatggc ccaaacgatc aaggattgtc
      181 ctcttctcat atcgccagca gtaaagatat ttggaatatt ggtttggaat ttaccaaatt
      241 ctgctttgta gttacttctg aaatcaagtt caatgcctaa ttggctgctc aatgtttttt
      301 caggtcctgt aaaacctaag gcaagcaaag ctaaatcgca aggccaagtt ttttcggaac
      361 ccgctacttc aattaattca ggacgttgac caggaaccat tttccattct acattaaccg
      421 tttttagagc gattaatttt cctttgtcat ctttgataaa ttctttagta ttgatcaacc
      481 agtttctttc gcaaccttct ttgtgagaag tagaagtttt taactgcaaa ggccaatacg
      541 gccaaggagt agattcgctt cttcctactg gaggttttgg cataatctca aagttagtta
      601 ccgatttagc tccgtgacga ttagaagttc caacgcagtc agatcctgtg tctccaccac
      661 caattacgat aacatctttt cctgttgcta atacttggtt ttctactttt tgaccaaaaa
      721 ctactttggt ttgttgtgtt aagaaatcca tagcttgaac tacaccgtct gcatcagctc
      781 ctggagttgg caaacctctt ctttcggttg ctccaccaca caatacgatg gcgtcaaaag
      841 ctttcaagtc gttaatatca tagtttacac ccacgtgagt gtttacttta aagctgatgc
      901 cttctgcttc taaaacggct attctacgat cgataattcc tttttctaat ttgaaattag
      961 gaattccgta gcgcaacaat cctccgatag catcatctct ttcgaagact gtaactaaat
     1021 gtccagctct gtttagttgt tgtgctgcgg ctaaaccagc tggtccagaa cctacaatag
     1081 caactgtttt tcctgttctt gttttgggtg gttgtggttt gatccatcct tctttgaagg
     1141 cacgttctac aatatttttt tctaaatttt caatagatac tggatcttct ataagaccta
     1201 aaacacatgc tttttcgcaa ggagcaggac acaaacgtcc tgtaaattct ggaaaattgt
     1261 ttgttgagtg taatatccaa gaggcttttt gccattcgcc ttggtgtacc atatgattga
     1321 aatcaggaat caaattgcct agagggcatc cgctatgaca aaatggtatg ccgcaatcca
     1381 tacaacgtga cccttgtttt gtaatatcag cttcactcag ggcaactgta aattcattgt
     1441 aatttactac acgttcttct actgcaatat atgattcatc tattctttcg aattctttaa
     1501 aaccggtaac ttttcccatt gtattatgct gttaattctt caaaaatttg ttcttctgtt
     1561 tccagacgtt tcaacgcttt tttgtactca gtaggcatta ctttaacaaa tttgcttagg
     1621 ctattagccc aatctgctaa taattcagcg cctcttgtac tatttgtata ggcaacgtgc
     1681 ttggctatca atgctttcaa ttcttcagct tcttcagctt caattgtttc aaaagctatt
     1741 gattcagtgt tgcataaacc gtttttgaat ttgttttctg gatcgtaaat gtaagcaaca
     1801 cctccactca ttccagcagc aaagtttctt ccagttctac ctaatacaac tactttacca
     1861 ccagtcatat attcgcaacc gtgatctcca actccttcaa caactgctgt tgctccagag
     1921 tttctaacgg caaaacgttc acctgcaata ccatttatgt aagcttctcc ttcgattgct
     1981 ccaaataagc aaacgtttcc aacaatgata ttgttttcag caacaaatgt cgcttttgct
     2041 ggttttttga taattaattt ggcaccagaa agtccttttc ctaaatagtc attcgtgttt
     2101 ccttcaacag taaatgtcaa tccgtgagca ctaaaagccc ctaaactttg accagcagaa
     2161 ccagtgaagt ttatgtttaa ggtgtcttcg ggtaaaccta ggtatccgta tatttttgaa
     2221 atttcattac ttacaatagc accaactgaa cgatcggtat ttttgatagg gtaattcaat
     2281 gtcatttttt cttttctgta cattgcacgg tgtgaatctt tcaagatttg gaaatcaagg
     2341 acattatcta aggcatggtc ttgtttttcg gtatttctaa caattctttc gctgtatccg
     2401 tcaggagtgt gcaaaatggc tgataaatct aaacctttag ctttataatg agtaatcgct
     2461 ttattggtat tgattttatg agtttgtccc accatttcgg ctagggttct aaagcctaac
     2521 tgagccataa tacctcttaa ttcttcggca acgtagtaga agaagttaat gacgtgttct
     2581 ggagttcctt tgaaattttt acgcaattct ttgtcttgag ttgcaatacc aactggacaa
     2641 gtatttaagt gacatttacg catcataatg cagcctgaag caacaagtgg agcagtagca
     2701 aaaccaaatt cttcagctcc taataaagca gcaattgcta catcacgacc cgtttttaat
     2761 tgtccgtcac attcaacaac gattctactt ctaaggttgt ttaaaactaa agtttgttga
     2821 gcttcggcca atccaagttc ccaaggaaga cctgcgtgtt tcaaggatgt taatggagaa
     2881 gctccagttc caccatcata acctgcaatt aacacaacgt ctgcttttgc tttagcaaca
     2941 ccagcagcaa ttgttccaac acctacttct gaaactaatt tcacattgat acgtgcttct
     3001 ctgttggcat ttttaaggtc gaaaattaat tgtgctaaat cttcgataga gtagatgtcg
     3061 tggtgtggtg gtggcgaaat caatcctaca taaggagttg agttacgagc ttctgcaatc
     3121 caaggcaata ctttttcgcc gggcaattgt ccaccttctc caggttttgc accttgagcc
     3181 attttaattt gaatttcttt tgcacttgat aaatagtgag aagttactcc aaaacgacca
     3241 gaagctactt gtttgatagc cgagttgcga ctgtcaccat tcatatcagg ttggaaacgt
     3301 tttctgtttt ctccaccttc accagaatta gattttccac caattcgatt catggctatt
     3361 gctaaattct cgtgtgcttc tctagagata gatccgtaag acatcgctcc cgttttgaaa
     3421 cgttttacga tatcagtcca aggctctact tcgtcaatag aaattggatt caagttgtca
     3481 aattcaaata aaccacgaat ggtcatcaaa ttatgtgctt gatcatttac tgctttcgaa
     3541 taggcgtcat aactagattg atcgcttaat cgaacagctt gttgt
//
LOCUS       DAOSNN010000256         3558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_26348_length_3558_cov_4.656580, whole genome shotgun sequence.
ACCESSION   DAOSNN010000256 DAOSNN010000000
VERSION     DAOSNN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26348_length_3558_cov_4.656580"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..1351
                     /locus_tag="PLQ70_10890"
     CDS             365..1351
                     /locus_tag="PLQ70_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQE35165.1"
                     /translation="MKENRKIRWGIIGLGNIAHQFAKDLLLVEDAELAAVASRDSEKS
                     KEFAEKYQCKKAYSTYDDIINDDNIDILYIATPHNSHASLTIKGLQNNKHILCEKPIA
                     LNYDDALQMIQASKANTKFFMEAFWTRFNPSFREAFSRIKKGEIGEIKYINADFAFYV
                     EKFGNIGDRKVDLKLGGGALLDIGVYPLFLCYIVLGIPTEIVAKSNFHTSGADLQTSM
                     ILQYENAQAILHSSFVSTSNIKATINGTKGRINLNTMWHEAQSYTLNIDNHEEEFLLP
                     TKGKGFTYEIEECHQCIKENRIESELWSHQNSLDLIKIVDLVKNQIGLEYPS"
     gene            1441..2976
                     /locus_tag="PLQ70_10895"
     CDS             1441..2976
                     /locus_tag="PLQ70_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQE35166.1"
                     /translation="MLVKVFGSAVFGVEATTITVEVNIDKGVGYHLVGLPDNAIKESS
                     YRIAAALKNNGYSLPVKKITINMAPADLRKEGSAYDLTLAIGILVASGQIKGDEIDQY
                     IIMGELSLDGSLQPIRGALPIAIKAKEEGFKGFFLPKQNVKEAAIVAGLDVYGVENVQ
                     EVIDFFEGKGSLEPTIIDTRAEFYKTLDFPEFDFSDVKGQESIKRCMEIAAAGGHNII
                     LIGPPGAGKTMLAKRLPSILPPMTLREALETTKIHSVAGKLKEVGLMNQRPFRSPHHT
                     ISNVALVGGGSYPQPGEISMAHNGVLFLDELPEFKRDVLEVMRQPLEDREVTISRAKF
                     TVTYPSSFMLVASMNPSPSGFFNDPDSPQTSSPHEMQRYLSKISGPLLDRIDIHIEVT
                     PVPFEKLSDDNKAESSVEIRKRVTAAREIQSERFVQMENIHYNAQMNTKHIREYCALD
                     DDSKQLLKTAMERLNLSARAYDRILKVARTIADLEAAPKVISSHIAEAIQYRSLDRDG
                     WLG"
     gene            3228..>3558
                     /locus_tag="PLQ70_10900"
     CDS             3228..>3558
                     /locus_tag="PLQ70_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35167.1"
                     /translation="MKNLIKLLNSNIIAENSNNETFQETLNPSDKDIIINLNYDVDGI
                     TKSECAKLLVFQNKNVYQNYTKWSENSSLYSNGKRYYFTGDTYDFLNIYVALIVPYSN
                     EYIVENKK"
BASE COUNT         1223 a          560 c          669 g         1106 t
ORIGIN      
        1 gcttcgccat agccagaata gccaccactt tgtaattcga ctattaagga aggttgtatc
       61 gtatgagatt ctcttgaaat ggtaaaagga tgttttaatt taagattaaa tgttctaagt
      121 attaatttca tttttaggac ttaaagttga ataaatgtta aatttataac aaagtaaagt
      181 attaaatgtc ggttttcaaa caaaaaatac aagtaaattt tattttgaaa tcaattttat
      241 gaaatagaaa aaattgctct attttaaatg cttttttatc ttttttagat tctcatttta
      301 ccaataaaag aaacatataa tgtatatttg aatctaaatc agttgagtat aaattcaaaa
      361 aaagatgaaa gagaatagaa agattcgttg gggaattatt ggtttgggaa atattgccca
      421 tcaatttgct aaagatttat tgttggtcga agatgctgag ctagcagccg ttgcttctag
      481 agattctgaa aaatcaaaag aatttgccga aaaataccag tgcaaaaaag cctattctac
      541 ctatgatgat attattaatg acgataatat tgacattcta tatatcgcaa cgcctcacaa
      601 ttcgcacgct tctttgacca taaaaggttt gcaaaataac aagcatattc tttgcgaaaa
      661 accaatagcc ttaaattatg acgatgcttt gcaaatgatt caagcatcaa aagccaatac
      721 taaatttttt atggaagctt tctggactcg atttaatcct tcttttcgag aagctttttc
      781 aagaattaaa aagggcgaga ttggagaaat caaatacatt aatgccgatt ttgcatttta
      841 tgtcgaaaaa tttggcaaca ttggagatag aaaagttgat ttgaaactag gcggaggagc
      901 acttttagat attggtgttt atcctttgtt tttatgttat attgtgttgg gaattcccac
      961 cgaaatagta gcaaaatcaa attttcatac atcaggagcc gatttgcaaa cttccatgat
     1021 tttgcaatac gaaaatgcgc aagccatttt gcattcgagt tttgtatcga cttccaatat
     1081 aaaagcaact ataaatggta ccaaagggag aattaatttg aatacaatgt ggcatgaagc
     1141 acaatcctat actttgaata ttgataatca tgaagaagaa tttctattgc caaccaaagg
     1201 caagggtttt acttatgaaa tagaagaatg tcatcagtgt attaaagaaa acagaattga
     1261 aagtgaactt tggtcacacc aaaatagttt agatcttata aaaatagtag atctggtcaa
     1321 aaatcaaatc ggattagaat atccttcctg attaaggaat atataaattg taacaattcg
     1381 aaaatatttt tcattttgaa aggaaattta gattaaattt agatttttaa ttgtacaatt
     1441 atgttagtca aagtttttgg aagtgccgtt tttggtgttg aagcaacaac cattaccgtt
     1501 gaagtcaata tagacaaagg agttggatat catttagttg gtttgcccga caatgcaatc
     1561 aaagaaagta gttaccgaat tgctgctgcg ctaaaaaata atgggtattc actaccggtt
     1621 aagaaaatta ccatcaatat ggcgcctgcc gatttgagaa aagaaggatc ggcttatgat
     1681 ttgactcttg ccattggaat acttgtggct tctggacaaa taaaagggga tgaaattgac
     1741 caatatataa ttatgggaga actttcgtta gacggaagtt tgcaacccat tcgtggcgct
     1801 ttacccattg ctattaaagc caaagaagaa ggatttaaag gtttttttct acccaaacaa
     1861 aatgtaaaag aagccgcaat tgttgctgga ttagatgttt atggagtaga aaatgtgcaa
     1921 gaagttattg atttcttcga gggaaaaggc agtttagaac ccaccataat cgataccaga
     1981 gccgagtttt ataaaacctt agactttccc gaattcgatt ttagcgatgt aaaaggacaa
     2041 gaaagcatca agcgttgtat ggaaattgca gccgctggcg gtcataatat aattctaatt
     2101 gggcctcctg gagcaggaaa aacaatgctt gccaaacgat tacctagtat tttgcctcca
     2161 atgactttgc gtgaagctct cgaaactacc aagatccata gtgtggctgg taagttgaag
     2221 gaagtgggtt tgatgaacca aagaccgttt cgcagtccgc accatactat ttcgaatgtg
     2281 gcacttgttg gaggaggaag ctacccacaa cccggcgaaa tttcgatggc gcacaatggc
     2341 gttttatttt tggacgaatt gcccgaattc aaaagagacg ttctcgaagt aatgcgccaa
     2401 cctttggaag atagggaagt gaccatttcc agagccaagt ttaccgtgac gtatccttcg
     2461 tcttttatgt tggtggcgag tatgaatccg agtccgagtg gcttttttaa cgatcccgat
     2521 tcgccacaaa cgtcttcgcc gcacgaaatg caacgttatt tgagcaagat ttcaggtcct
     2581 ttattagaca gaatcgacat tcatattgaa gttacaccag ttcctttcga aaaattatcc
     2641 gatgacaaca aagccgaaag cagtgtcgaa atcaggaaac gagtgaccgc tgcgagagaa
     2701 attcagtcag aacgtttcgt gcaaatggaa aatattcatt acaatgccca aatgaatacc
     2761 aaacacatcc gagaatattg cgctctcgac gatgattcca aacaattact aaaaactgca
     2821 atggaacgtt tgaatctttc ggcacgagcc tacgacagaa tcctgaaagt agccagaact
     2881 atcgctgact tggaagccgc acccaaggtt atttcctccc acattgccga agctattcag
     2941 tatagaagtt tggatcgtga tggttggttg gggtagtttc agattatatg ctccgtaaag
     3001 tctccgactt tgaggtagtg attttcggtt tccaaccgat tttgtttcgg attgtttaat
     3061 ttaaatgtca gtcagagact gacgaacaca tccacaaagt cggagacttt gcggagcgat
     3121 attgttttta gttattaata tgtagttaga tatagaataa agctatcctc taagttgttt
     3181 taaattctca atttagtaat ctatttttgc tatctaaata aatcaatatg aaaaacttaa
     3241 ttaaattatt aaacagtaat ataattgctg aaaattcaaa taatgaaaca tttcaagaaa
     3301 cattgaaccc tagtgataaa gatataatta tcaatcttaa ttacgatgtc gatggtataa
     3361 ctaaaagtga gtgtgcaaaa ttacttgttt tccaaaataa aaatgtatat cagaattaca
     3421 caaaatggag tgaaaattct tcactttata gtaatggaaa aagatattat tttactggag
     3481 atacttatga ttttttaaat atttatgtcg ctttaatagt tccatattct aatgaatata
     3541 ttgttgagaa taaaaaat
//
LOCUS       DAOSNN010000257         3536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_26570_length_3536_cov_4.863258, whole genome shotgun sequence.
ACCESSION   DAOSNN010000257 DAOSNN010000000
VERSION     DAOSNN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26570_length_3536_cov_4.863258"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2755)
                     /locus_tag="PLQ70_10905"
     CDS             complement(<1..2755)
                     /locus_tag="PLQ70_10905"
                     /inference="COORDINATES: protein motif:HMM:NF027309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HQE35168.1"
                     /translation="MKNTILTFLFILLFLSKTEAQTEIASKFEVFTKDQKASILYDKA
                     ITLDSILAYSLAEDIFKVSGFKPLVTQNKAAVSGNVIVIGRIQSKLVQSFLDKKNDFK
                     NFEKQWESYGYKIILNPNSKITKAFIIAGTDPRGTAFGVFDLSQKMGVSPWYWWTDVP
                     VAKQKELILTQNSFQSKAPSVKFRGIFLNDEDWGLRPWAAKIFEPEIGNIGPKTYAKI
                     FELLLRLKANTIWPAMHEGTTGFFKVPGNAKIAEDYQIVVGTSHAEPMLRNNVDEWKE
                     KEMGHFNYVSNRDNVLKYWEDRVKESKNVDAIYTIGMRGVHDSKMEGVKDNKEGAALL
                     KQIIIDQRKLLETYINRDATKVPQAFTIYKEVLDLYDEGLKVPEDITLVWTDDNYGYI
                     RRLSNPEEQKRPGGGGVYYHASYWGRPHDYLWVDSVHPALLREEMMKAYQSNCKEIWI
                     LNVGDLKSIEYSTTLFLDMAYDAPFFEDASNVKKHMTAFYSSIFGEIYGKSIADLKWE
                     YFDLAFERKPEFMGWSQTEPTTKTKQTAYNLFFFGDENQRRIDQYQNLENQADAIKSK
                     LPKELQEAYFQLVYYPTKAASLMNKKFLYADKANIYGKQGRLNAKDFALKSENAFNEI
                     KAITQEYNQIGNGKWNFIMDMSPRKLPVFDKANIEIPLQTDGVDSAGILVENETDSSP
                     NESKLTYSQDSNTSHFVDLFLKKDAVVSWQLQNLPDWIIANKKTGILNVATNNSERIQ
                     ISIDWNQWKKNPIEKAQFSIVYGEKKKVITLEIDSNNAAVAKNSFVAIDNKVVIYAQN
                     FTNIKNQGAYEWKKIDGLGYSKAAMQAYPPTALFQTDSSSYLEYHIHLDNLNSLDAEL
                     FLYAIPTLPLTDKNGVRVGVQWNNEPMQIVDFATVGRSEEWKQNVLSNKATKKIALKS
                     FNKG"
     gene            complement(2930..>3536)
                     /locus_tag="PLQ70_10910"
     CDS             complement(2930..>3536)
                     /locus_tag="PLQ70_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE35169.1"
                     /translation="ATDGPGWNHTLANNLGFAATTSELTDIDKTRCFLTTNYFDMSVT
                     VNSADFLSTDETLLTAPREADGSLPKNNFLKLKAGSDIINAGTDIGFAFKGAAPDLGC
                     FEFDSSLGLKDFTEKKGIFNYPNPFSVETNVVFQNNQNAKLSIELYNMMGMKVMEIPA
                     KTYPNGENTITIKRDGLAAGNYVLVIKGDDSERSSKLITIQ"
BASE COUNT          975 a          713 c          631 g         1217 t
ORIGIN      
        1 ttcctttatt aaaagatttc agggctattt ttttggttgc tttattagac agaacgtttt
       61 gtttccattc ttcgcttctt ccaacagttg caaaatcaac tatctgcata ggttcattat
      121 tccattgcac gcctacacga actccgtttt tgtctgttag aggaagggtt ggaatggcat
      181 ataaaaacaa ttctgcatct aaactattca aattatctaa atgaatatga tattctaagt
      241 aagaagagga atcagtctga aaaagtgcag ttggcggata agcttgcatc gccgctttgc
      301 tataacccaa cccatctatt ttcttccatt cataggctcc ttgatttttt atgttagtga
      361 agttttgcgc ataaatcacc actttattgt caattgctac aaaagagttt ttagcaacag
      421 ctgcattatt ggaatcaatt tcaagagtga tgactttctt tttttcgcca taaacgatgc
      481 tgaattgcgc tttttcaatt ggatttttct tccattgatt ccaatcaata ctgatttgga
      541 ttcgttccga attgtttgtt gcaacattca aaattccagt ttttttattg gcaataatcc
      601 aatccggaag gttttgcaat tgccacgaaa ccaccgcatc tttctttaga aataagtcaa
      661 caaaatggga agtattcgaa tcttgagaat atgtcagttt actttcattt ggactagaat
      721 cggtttcgtt ttctaccaaa atccctgctg aatcaacgcc atcagtttgc aatggaattt
      781 caatatttgc cttatcaaaa acgggcaatt ttcgtggact catatccata atgaagttcc
      841 attttccgtt cccgatttga ttgtattctt gggtaatggc cttgatttcg ttgaacgcat
      901 tttccgattt caaggcaaaa tccttggcat ttagtctccc ttgtttgcca taaatgttgg
      961 ctttgtcggc atacaaaaac tttttgttca tcaacgaagc cgcttttgtt ggataataaa
     1021 ccaactggaa ataggcttct tgaagttctt ttggcaattt gcttttgatg gcatcggcct
     1081 ggttttccaa attttggtat tggtcaattc ttctctggtt ttcatcccca aaaaagaaca
     1141 gattgtaagc tgtttgcttg gtttttgtag tcggttcggt ttggctccag cccataaatt
     1201 cgggttttct ttcaaatgcc aaatcgaaat actcccattt caaatctgca atcgactttc
     1261 cgtatatttc cccaaaaatc gacgaataaa aagcagtcat atgttttttg acattagacg
     1321 catcctcaaa aaatggtgca tcatacgcca tatccaaaaa aagcgtggtc gaatattcaa
     1381 tcgatttcaa atcgcccaca ttcaaaatcc agatttcttt gcaattggat tgataggctt
     1441 tcatcatttc ttcccgaagc aaggcaggat gaaccgaatc aacccaaaga taatcgtgcg
     1501 gtcttcccca ataggaagcg tggtaataaa cgccacctcc gccgggacgc ttttgctcct
     1561 cgggattgct caatcttcgg atgtagccgt aattgtcgtc ggtccaaacc aaggtgatgt
     1621 cttcgggaac tttcagtcct tcgtcgtaca aatccaggac ttctttataa atcgtaaaag
     1681 cctgcggaac cttggtcgca tcccgattga tgtaggtttc cagtaatttt ctttggtcta
     1741 tgataatttg tttcaataat gcggcacctt ctttgttgtc ttttacgcct tccattttgc
     1801 tgtcgtgaac gccacgcatt ccgatggtgt aaatggcatc gacatttttg ctttctttta
     1861 ctcggtcttc ccagtattta aggacattat ctctattgga aacataatta aaatgcccca
     1921 tttccttttc tttccattca tcgacattgt tgcgcaacat cggttcggcg tgcgaggttc
     1981 ccaccacaat ttgataatct tcggctattt tggcattgcc tgggacttta aaaaaacctg
     2041 tcgttccttc gtgcattgcc ggccaaatcg tgttggcttt caagcgcaac aacaattcga
     2101 atattttagc ataggtttta ggtccgatat tcccaatttc aggttcgaaa attttggctg
     2161 cccaaggtcg caatccccaa tcttcatcgt tcaggaatat tcccctgaat ttgaccgatg
     2221 gcgctttgga ttgaaatgaa ttttgggtta aaatcaattc tttttgtttt gcaacaggca
     2281 cgtcagtcca ccaataccaa ggtgaaacgc ccattttttg gcttaaatca aaaactccga
     2341 aagccgttcc tctagggtcg gttccggcaa taataaaggc tttggtgatt ttagaattgg
     2401 gatttagaat tattttgtag ccataacttt cccattgttt ttcgaaattt ttgaaatcat
     2461 tttttttatc caaaaaagat tgcactaatt tcgattgaat ccgtccaatg acgatgacat
     2521 tgcccgatac agcagccttg ttttgggtaa ccaagggttt gaaaccgcta actttgaaaa
     2581 tatcttccgc caaggaataa gccaaaatcg aatccaatgt tatggctttg tcgtataaaa
     2641 tgcttgcttt ttggtctttg gtaaacactt caaattttga tgcaatctca gtttgagcct
     2701 ccgtttttga aaggaacaat agaataaaca aaaaggtcag gatggtattt ttcattatat
     2761 aaaatgtaat tgaatcataa tttattccgt ttgtcaagct ggaagtgtct agattatgtt
     2821 gctctcgtgc gaggagacgc ttccagcgtg acaaatagca agaaaaacca cagttgttta
     2881 ggctggttaa ttgattttaa agttgatttt cagctaaatt taaaaactat tattgaatgg
     2941 tgattaattt actacttctt tcagagtcat cgcctttgat aactaaaacg taattaccag
     3001 cagctaatcc atcacgtttt attgtaatcg tgttttcgcc atttggatag gtctttgctg
     3061 gaatttccat cactttcatt cccatcatat tgtataattc aatggacaat ttagcatttt
     3121 gattgttttg aaaaacaaca ttagtttcaa cagaaaacgg atttggataa ttgaatattc
     3181 ctttcttttc ggtaaaatct ttcagtccta atgaactatc aaattcaaaa caacccaaat
     3241 ccggtgctgc tcctttgaac gcaaatccaa tatcggttcc ggcattaatg atgtcgcttc
     3301 ccgcttttaa ttttaagaaa ttatttttag gcaaacttcc atcggcttct cttggagcgg
     3361 ttagtaatgt ttcatctgta cttaaaaaat cagcactatt aaccgttacc gacatatcaa
     3421 aataattggt agtcaaaaag caacgggttt tgtctatatc tgtcaattcg gatgtggtcg
     3481 cagcaaaacc taaattattt gccaaggtat gattccatcc agggccatca gtggca
//
LOCUS       DAOSNN010000258         3531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_26612_length_3531_cov_6.310414, whole genome shotgun sequence.
ACCESSION   DAOSNN010000258 DAOSNN010000000
VERSION     DAOSNN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26612_length_3531_cov_6.310414"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="bioA"
                     /locus_tag="PLQ70_10915"
     CDS             <1..966
                     /gene="bioA"
                     /locus_tag="PLQ70_10915"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HQE35170.1"
                     /translation="KVFFSDNGSTSVEVAIKVALQYFFNKREKKTTIIAFENAFHGDT
                     FAAMAASGISFYTQAFQGMFIDVVRIPVPTKGQEQESFYALRNVIKKHNCAGFIFEPL
                     VQGAAGMVMYEPESLNELIRICKKNKVLTIADEVMTGFGKTGKTFACDYLTETPDMMC
                     LSKALTGGTIPMAITTFTQDIFEAFYDEDINKALFHGHTFTANPTGCAAALASLELLQ
                     TQEMQDNIARVNQRHLAFQKQIETHPKVATTRVLGTIFALEIKTESAASYYGSLRNKL
                     YDFFIENGVILRPVGNIVYILPPYIITDEQLQKVYEVVEKVLKIV"
     gene            complement(1079..2164)
                     /gene="bioB"
                     /locus_tag="PLQ70_10920"
     CDS             complement(1079..2164)
                     /gene="bioB"
                     /locus_tag="PLQ70_10920"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HQE35171.1"
                     /translation="MSITRHDWTKEEIIAIYNKPLMELLYEAATIHREHHDPNVVQIS
                     TLISLKTGGCSEDCGYCPQAKRYHTNIETNPFMTVEDVKVQAVKAKEGGSSRVCMGAA
                     WRNVKDGPEFDQVLEMVRTINKMDMEVCCTLGMITENQAQRLAEAGLYAYNHNIDTSE
                     EYYKEVISTRGFEDRLQTIENVRKTNITVCSGGIIGMGESIEDRAGMLKALSTLDPMP
                     ESVPINALVPVAGTPLEAEQSVEIWDMIRMIATTRIVMPATQVRLSAGRINWNREGQA
                     LCFFAGANSIFSGDKLLTTPNNDLNEDMKMFELLGLKNQQPFAKTYKRVSVEAADSKF
                     APLGEKPKWSRPGHTIERNVEATVKGK"
     gene            complement(2332..3261)
                     /locus_tag="PLQ70_10925"
     CDS             complement(2332..3261)
                     /locus_tag="PLQ70_10925"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQE35172.1"
                     /translation="MTVFNSINDFNCAKKTVLTLGTFDGVHIGHRKILEKLTQNTENG
                     KYENLVLTFFPHPRMVLQGQSDVELLNTIDEKIDLLEKIGIQNLVIHPFDETFSQLSA
                     EDFVKTILVDRFQIQKIIIGHDHRFGRNRTANIDDLIAFGEKYNFEVEQISVQEIQDV
                     SISSTKIRNALTEGNMALANEFLGYEYFLTGTVSKGKQLGRTIGFPTANLQIKKNYKL
                     IPKKGVYVIQSIINQKNVFGMMNIGFNPTVAGKQLAIEVHFFDFDTDLYHQEIRISIL
                     KYLRSEHKFESVALLKEQLEKDKNTALAYIESL"
BASE COUNT         1049 a          600 c          665 g         1217 t
ORIGIN      
        1 aaagtctttt tctccgacaa tggttcgact tcggtggaag ttgcgattaa agtagcttta
       61 caatattttt tcaacaaaag agaaaagaaa accacgatta tagctttcga aaatgctttc
      121 catggtgata cttttgcggc gatggcggct agtggaattt ctttttatac tcaagctttt
      181 caaggaatgt ttattgatgt ggtgcgaatt cctgtgccaa ctaaaggtca ggaacaagaa
      241 agtttttatg ccttgagaaa tgtcataaaa aaacataatt gcgcgggatt tattttcgaa
      301 cccttggtgc aaggagcagc tggaatggta atgtatgagc cagaatcatt gaacgaacta
      361 atacgaattt gcaagaaaaa caaagtcctc acgattgccg acgaagtaat gacgggtttt
      421 ggaaaaacag gaaaaacgtt tgcttgtgat tatctaaccg aaacgcccga tatgatgtgt
      481 ttgtccaaag cattgactgg cggaacgatt ccaatggcga ttaccacatt tacgcaggat
      541 atttttgaag ctttttatga tgaagatatc aataaagcgt tatttcacgg acatactttt
      601 actgccaatc caacgggttg tgcggcagct ttggcaagtt tagaattgtt gcaaactcaa
      661 gaaatgcagg ataatattgc ccgagtaaat caaagacatt tggcttttca aaaacagatt
      721 gaaacgcatc caaaagtggc tacaacccga gtgttgggaa ctatttttgc cttggaaatc
      781 aaaaccgaaa gcgccgctag ttattacgga agcttacgaa ataaactcta cgatttcttt
      841 attgaaaatg gagtgatttt gcgccctgtt gggaatatag tttacatttt gccaccttat
      901 atcattactg atgaacaatt gcaaaaagtg tacgaagtgg tcgaaaaagt attgaaaata
      961 gtttagaaat tatttctata accaattaat atgatagtct ttgatcatga atgaataccg
     1021 aattgcatat agtttgcttt aatagactaa atgcaattca gtattctcat aaatattttt
     1081 attttccttt tacagttgct tccacatttc tttcgatagt gtgtccagga cgagaccatt
     1141 ttggtttttc gccaagagga gcaaatttag aatcggccgc ttcaacagaa actcttttat
     1201 aggtttttgc aaaaggttgt tggtttttca atcccaataa ttcaaacatt ttcatgtctt
     1261 cgtttaagtc attattagga gtagtaagta atttatcacc tgaaaaaata gaattggcac
     1321 cagcaaaaaa gcacaaagcc tgaccttcac gattccaatt tattcttcct gcagaaaggc
     1381 gcacctgagt ggctggcatt actattcttg ttgtagcaat cattcgaatc atatcccaga
     1441 tttcgacaga ttgttctgct tcaagtggtg ttcctgcaac tggaactagt gcatttattg
     1501 gcaccgattc tggcattgga tccaaggtag agagagcttt aagcattcct gctcggtctt
     1561 cgatgctttc acccattccg ataattccac cactacaaac tgtaatattg gttttacgaa
     1621 cattttcgat ggtttgcaaa cggtcttcaa atccacgggt cgaaatgact tctttataat
     1681 attcttcaga agtgtcgata ttgtggttgt aggcatataa accagcttcg gccaatcgtt
     1741 gtgcttggtt ttcggtaatc atacccaacg tacagcacac ttccatatcc attttgttga
     1801 tggtacgaac catttctaaa acttggtcaa attcagggcc gtcttttaca tttctccaag
     1861 cagcacccat acaaactctt gatgaacctc cttcttttgc tttgacagct tgtactttta
     1921 catcttcaac agtcataaaa ggattggttt caatattggt gtggtagcgt ttggcttgcg
     1981 gacaatagcc acaatcttct gagcaaccgc ctgtttttag tgatattaaa gtagatattt
     2041 gaactacgtt tggatcgtgg tgttctctgt gaatggtagc tgcttcataa agcaattcca
     2101 ttaggggttt attatatatg gcaataattt cttcttttgt ccagtcgtgt cttgtaatac
     2161 tcatcgatct tattttttag tgtgtcaaaa gtagttaaat tgttccaatc aagcaatgat
     2221 gtagtattga aaagtattgg actttaatta ttgggattgt cttcttattg gagtttttca
     2281 acaatgggaa tgcaattaat tttatttgaa gaaaaaagaa tctgggtaaa tttaaagact
     2341 ttctatataa gctaaagctg tatttttgtc tttctctaat tgttctttta gtaaagctac
     2401 cgactcgaat ttatgttcgg agcgaagata tttcaggata gaaattctta tttcttgatg
     2461 gtataaatca gtatcaaagt caaaaaagtg gacttcaata gcaagctgtt ttcccgcaac
     2521 agttggatta aatccaatat tcatcattcc gaagacattt ttttggttga tgatgctttg
     2581 tataacataa accccttttt tgggaatgag tttgtaattt tttttaattt ggaggtttgc
     2641 cgtgggaaat ccaatggttc ttcctagttg ttttccttta gaaactgttc cagtaaggaa
     2701 atattcataa cccaaaaact cattggccaa agccatgttt ccttcggtta aggcatttct
     2761 gattttggtc gaacttatcg aaacgtcctg tatttcttga accgaaattt gttctacttc
     2821 aaaattatat ttttcaccaa aagcgatcaa atcgtcaata ttggcagttc ggtttcttcc
     2881 aaatcgatgg tcgtgaccaa ttattatttt ttgaatttgg aatcgatcta ctagtatagt
     2941 ttttacaaaa tcttcggcag ataattgaga aaaggtttcg tcaaaaggat gaataaccag
     3001 gttttgaatg cctatttttt ctaataaatc tattttttca tcgatggtgt tgagtaattc
     3061 cacatccgat tgtccttgca ataccattct tggatgcgga aaaaaggtaa gtaccaggtt
     3121 ttcgtacttg ccattctcag tgttttgagt tagtttttct aatatttttc gatgaccaat
     3181 gtgcacacca tcaaaagtgc ccaaagtgag aacggttttt ttagcgcaat tgaaatcatt
     3241 tatagaattg aaaacggtca aagcaatttg tttttagaat gctgcaaatt tatgctaata
     3301 aaacagataa aaaaaagagg attgatagat aaatcaaccc tcttaattgg tttgtgttat
     3361 gaaattagtc tttgatcaac tgcaatgttt ttgcttggtc gtctttggta actgttataa
     3421 aatatatccc tttgctataa gatgaagtgt ttagtgttaa atcgtctgct tttgaaactg
     3481 cttttcggct aatgatttga cctaagctat tgctaatagt taaacttgtt g
//
LOCUS       DAOSNN010000259         3497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_26951_length_3497_cov_4.540093, whole genome shotgun sequence.
ACCESSION   DAOSNN010000259 DAOSNN010000000
VERSION     DAOSNN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26951_length_3497_cov_4.540093"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1759)
                     /locus_tag="PLQ70_10930"
     CDS             complement(131..1759)
                     /locus_tag="PLQ70_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HQE35173.1"
                     /translation="MKKLFFFISFGLAVLSFQNLLAQAPKKILVEHSDYFDVNQVEAP
                     DAALLTGNVRVNHDGIIMTCNKAYYFQKENYIKAFGDVQLVQGDTLFLNSKYAEYNGN
                     MRKAFATGNAKMRSPESTLVTDTINFDRNTQEVFYNTNGTITNRDNTLKSKSGKYYVA
                     EKKFQFLTAVTITNPKYVIKSNHLDYYSNSGHSYLFGPSTITSKTNYIYTEKGFYDTK
                     KNVSHFLRKSYIKYDDRLIEGDSLYYDRNKEFASATRNVKIKDSVNRGIIKGHYAELY
                     KNKDSMFVTKRAVAVNFVENDSVYIHGKKLLVTGPEGNRIIRGFNNVRFYKTDMSGKC
                     DSIHSSTKTALTKLIGNPILWNGESQITGDVMHLIGDQKTQKLDSLKVLNNTFIISKD
                     TIGTGFNQVKGQNLFGKFLEGKLHEVDIIKNAEVIYYMRNDAQELIGINKNVSSKINI
                     IFDKKEIETITFFKQVDGTIYPEKDLPEPDRKLKGMVWRGDERIKSKDDIFPPEENID
                     NEKLVQQGKDEKAKENVPMKIRKETLNYDKKKKK"
     gene            2042..>3497
                     /locus_tag="PLQ70_10935"
     CDS             2042..>3497
                     /locus_tag="PLQ70_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HQE35174.1"
                     /translation="MMKKITIVASISVLLLATACKTTAMKMDTEKKKTPQKKEVVYQV
                     FTRLFGNQNTTNKPWGTIEENGVGKFNDFTDKALKEIKDLGVTAVWYTGVPHHALIRD
                     YTSIGISNDDPEVVKGRAGSPYAVKDYYNVNPDLAVNPTNRLQEFEALIARTHKAGLK
                     VIIDIVPNHIARKYEGKSNPKGVRDFGADDDVSVEYKRDNNFYYIPNTRFEIPDAGKP
                     LNGESNPLIDGKFEEFPAKWTGNGSRLAKPDKNDWYETVKVNYGIRPDGSKDFPELPA
                     GFDTKSYQEHFDFWKDKSVPNSWIKFRDIALYWTAKGVDGFRYDMAEMVPYEFWSYMN
                     SAIKVKNPDAFLMAEVYNPNEYRNYIRLGKMDYLYDKVETYDHLKAVIQGKTWPDGLS
                     DIQHRMADIEHNMLKFLDNHDEQRLASPEFAGTPEKGKPLMVVSATISTAPTMIYFGQ
                     EVGEAGNENAGFGTHSRTSIFDYIGVPNHQRWMNG"
BASE COUNT         1068 a          572 c          675 g         1182 t
ORIGIN      
        1 taaccctata aaagaaagta ttccgtattt gatgtaattg ttgttcaata gttatttgtt
       61 ttttatgaaa ttatttcgtt cttcgcaata acattacaac tgaaaactgc gacttaaaac
      121 tgaccactga ttatttcttt ttcttcttgt cgtaattcaa ggtttctttc ctgattttca
      181 tcgggacatt ttccttggct ttttcgtctt ttccttgctg gactaatttt tcgttatcaa
      241 tattttcttc aggaggaaaa atatcgtctt tcgatttgat tctttcatct ccgcgccaga
      301 ccattccttt tagttttcga tcgggttcgg gcaggtcttt ttcgggataa atagtaccat
      361 caacctgttt gaaaaaagta atggtctcta tttctttttt atcaaaaata atattgattt
      421 tactactcac gtttttgtta ataccaatga gttcttgggc atcatttcgc atataataaa
      481 tgacttccgc atttttgatg atatcgactt catggagttt tccttccagg aattttccaa
      541 atagattttg tcccttgact tggttaaaac ctgttccgat ggtgtctttt gagattataa
      601 aagtattatt caagactttt agtgagtcta atttttgggt tttttggtcg ccaataaggt
      661 gcataacatc gccagttatt tgactttctc cattccaaag aataggattt cctatcaatt
      721 ttgtcaaggc ggttttggta ctggaatgaa tggaatcgca tttgccgctc atatcggttt
      781 tatagaaacg aacattgttg aaaccacgta taattcggtt gccttctggg ccagtaacca
      841 ataatttttt tccgtgaatg taaaccgaat cgttttcaac aaagttaact gccacggctc
      901 ttttggtcac aaacatcgaa tctttgtttt tgtacaattc ggcataatgt cctttgataa
      961 ttcctcgatt gacggaatct tttattttta catttcgtgt tgccgaggca aattctttat
     1021 ttcggtcgta gtacaaacta tctccttcaa tgagtcggtc atcgtatttg atgtaggact
     1081 ttcgaagaaa atgcgatacg ttttttttgg tatcataaaa ccctttttcg gtataaatat
     1141 aattggtttt actagtaatt gtcgatggtc caaaaaggta agaatgtccg gaattgctgt
     1201 aataatccaa atgattggac ttgattacat atttaggatt tgtgatggtt accgccgtga
     1261 ggaattggaa ttttttttcg gcaacataat attttccaga tttacttttt agtgtattat
     1321 ctctgttggt aatggttccg tttgtgttgt aaaaaacttc ttgtgtattt cggtcaaagt
     1381 tgatagtgtc cgttacaaga gtagattctg gtgaacgcat tttggcattt cctgtagcaa
     1441 aagcttttct catattgcca ttgtattcgg catatttact gtttaaaaat aaagtgtcgc
     1501 cttgcactaa ttgtacatct ccaaaggctt tgatgtagtt ttctttttga aaataataag
     1561 ctttgttgca agtcataatg atgccatcgt gattgacacg tacatttcca gttagcaatg
     1621 cagcatctgg agcttcgact tggtttacat caaaatagtc ggaatgttct accagtattt
     1681 tttttggggc ttgtgccaaa aggttttgga aacttaaaac ggctaagcca aaacttatga
     1741 aaaagaataa tttcttcaat ggatttattt tttgccaaat ttaagaaaaa ggaatcaacc
     1801 tgaattcata ttatgattta tttataactc cttacaattg atttgaggag cattcgattt
     1861 agaaaaaatt attaacaaaa aagaataata tttcgttgta gttccaattc tagcaaatag
     1921 attcaggtaa atattgatga attctcaaat tgatgccaaa aaaatagtat ttttaaaagt
     1981 tgttaatcca tttagattgg atttcataat tagcactaaa aatcaggata aataatttac
     2041 tatgatgaaa aaaataacta ttgttgcaag tataagcgta ttacttttgg caacagcttg
     2101 caaaactaca gctatgaaaa tggatacaga aaagaaaaag acaccgcaga agaaagaggt
     2161 tgtatatcaa gtgtttacta ggttgtttgg gaaccaaaat acgaccaata aaccttgggg
     2221 aactatcgaa gaaaatggag tagggaagtt taatgatttt accgataagg ccttgaaaga
     2281 aatcaaggat ttgggtgtta cggctgtttg gtacacggga gttccacacc acgctttgat
     2341 tcgcgattat acttcgattg gtatttcgaa cgatgatccc gaagtggtaa aaggtcgtgc
     2401 cggctcgcca tacgccgtga aagattatta taatgtaaat ccagatttgg cggtaaatcc
     2461 taccaatcgt ttgcaagaat ttgaagcctt gattgcccga actcataaag cgggattgaa
     2521 ggtaattatt gatattgttc ccaatcatat tgcccgaaaa tacgaaggaa aaagcaatcc
     2581 aaaaggggta agagattttg gtgccgatga tgatgtttcg gtagaataca aacgcgataa
     2641 taacttttat tatattccaa atacccgttt tgaaatcccc gatgcaggca aacctctaaa
     2701 cggggaaagc aatccactaa ttgatggaaa atttgaggaa tttccagcaa aatggactgg
     2761 taacggttcg agattagcaa aaccagacaa gaacgattgg tacgaaaccg tgaaagtaaa
     2821 ttacggaatt cgtcctgacg gttcgaaaga ttttccagaa cttcctgcag gttttgatac
     2881 taaatcctat caagaacatt ttgatttttg gaaagacaaa agcgtaccaa attcttggat
     2941 caaattccgt gatattgctt tgtactggac cgccaaaggc gtagatggtt ttcgttatga
     3001 tatggccgaa atggtgcctt atgaattttg gagctatatg aattcggcga taaaagtgaa
     3061 gaatcctgat gctttcttga tggccgaagt gtataatcca aacgaatatc gcaattatat
     3121 tcgtctcgga aaaatggatt atttgtatga taaagttgaa acctacgatc atttgaaagc
     3181 cgtaattcaa ggaaaaactt ggcctgatgg actttcggat attcaacata gaatggctga
     3241 tatcgagcat aatatgctta agtttttaga caatcacgat gagcaacgtt tggcaagtcc
     3301 tgaatttgct ggaactcctg aaaaaggaaa accactaatg gtagtttcgg caacgataag
     3361 tactgcgcca acaatgattt attttggtca ggaagtggga gaagccggaa atgaaaatgc
     3421 aggttttgga acccattccc gaacttccat ttttgattat atcggagttc caaatcatca
     3481 acgatggatg aacggag
//
LOCUS       DAOSNN010000260         3459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_27334_length_3459_cov_5.167156, whole genome shotgun sequence.
ACCESSION   DAOSNN010000260 DAOSNN010000000
VERSION     DAOSNN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27334_length_3459_cov_5.167156"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1528)
                     /gene="purH"
                     /locus_tag="PLQ70_10940"
     CDS             complement(2..1528)
                     /gene="purH"
                     /locus_tag="PLQ70_10940"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQE35175.1"
                     /translation="MSTTKKIQSALISVFSKEGLEPIIKKLHEQNVTFYSTGGTEEFI
                     KNLGIPVVPVEDVTSYPSILGGRVKTLHPKVFGGILNRQDNESDVQQMKEYDIPQIDL
                     VIVDLYPFEKTVASGASESDIIEKIDIGGISLIRAAAKNFKDTVIVPSVAEYSLLLDL
                     ISTQNGATTLEDRKMFATKAFHVSSHYDTAIFNYFNTDETIFKTSIANGQVLRYGENP
                     HQKGFFFGDFDAMFNKVHGKELSYNNLLDVDAAVNLINEFKNDGPTFAILKHNNACGL
                     ATRSTIAEAYNAALACDPTSAFGGVLIANTKIDVAAATEINKLFCEVVIAPSYDEAAL
                     DSLQEKKNRIILIQNEVELPQKQVRTCLNGVLVQERNNITDTKADLKTVTITAPTTQE
                     VEDLIFASKVCKNTKSNTIVFAKNGTLISSGTGQTSRVDALMQAVDKAKAFGFSLEGA
                     SMASDAFFPFPDCVQLAKEAGITAVIQPGGSIKDQLSIDYCNENKVAMVFTGTRHFKH
                     "
     gene            1693..2940
                     /locus_tag="PLQ70_10945"
     CDS             1693..2940
                     /locus_tag="PLQ70_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE35176.1"
                     /translation="MLVYFRLLSESFRFAMNALRNNKLRTLLSLLGVTIGIFSIIAVL
                     AAVDSLDRKITKDLSSLDKNTIYLMKMSFGPSEIPQWKREQFPNVKYDEYLYLKGAMT
                     NTDQMGYQIFTKSESLKYGSNTVTSVNVVPVSHEFIDIQGLEFDEGRFYTESEANSAA
                     PVVVIGNEIAKSLFEDSEPIGKKIRMYGQRFTVIGVLKKQGSGMFGNDDSSAFIPVNF
                     VRQQFGDNNKGLVNVIIFKPEKGVDMDAYKAEISQKLRAFRGVKAGEIDNFFINVFSG
                     FTDFIDGIISQMNVVGWIISGFSLLVGGFGIANIMFVSVKERTNLIGIQKSLGAKNRF
                     ILFQFLFEAIILSVIGGIIGLLLVWIIAMILTKALDFEFVLGMGNILLGTGLAAIIGL
                     ISGILPAISAAKLDPVEAIRTGM"
     gene            complement(3160..>3459)
                     /locus_tag="PLQ70_10950"
     CDS             complement(3160..>3459)
                     /locus_tag="PLQ70_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HQE35177.1"
                     /translation="AQLADAKIPYISLCTDPTTGGTTASYAMLGDINISEPGALIGFA
                     GPRVVKDTTGKDLPEGFQTAEFVMEHGFLDFITPRKELKDSINLYIDLIQNIDIR"
BASE COUNT         1025 a          559 c          642 g         1233 t
ORIGIN      
        1 attaatgttt aaaatgacgt gttcctgtaa ataccattgc aactttattt tcattgcaat
       61 aatcaatact caattggtct tttatagaac ctcctggttg aattacagct gttattccag
      121 cttcttttgc caattgtaca caatctggaa atggaaagaa tgcatcactg gccattgaag
      181 caccttctag gctaaaacca aaagcttttg ctttatcgac agcttgcatc aaagcatcta
      241 ctcttgaagt ttgtcccgtt cctgatgaaa ttaaggtgcc gtttttggca aaaacaatcg
      301 tattcgattt ggtgtttttg caaactttag acgcaaaaat caaatcttct acttcttgcg
      361 tagttggtgc agttatggta acggttttta agtctgcttt agtatcggta atgttgtttc
      421 tttcttgaac taaaactccg tttaggcaag tgcgcacttg tttttgtggc aattctactt
      481 cgttttgaat taagataatt ctgtttttct tttcttgtaa gctgtctagt gctgcttcat
      541 catagcttgg agcaataaca acttcacaaa ataatttgtt gatttcggtt gcagcggcta
      601 catcaatttt ggtattggca atcaaaactc ctccaaaagc agaagttgga tcgcaagcta
      661 atgctgcatt ataagcttcg gcaatagttg accttgttgc caatccgcaa gcgttattgt
      721 gttttaaaat ggcgaaagtt gggccatcgt ttttgaattc gttaatcaaa tttacggctg
      781 catcaacgtc taataaattg ttgtatgaca attcttttcc gtgaacttta ttaaacatgg
      841 cgtcgaaatc accaaagaaa aatccttttt ggtgtggatt ttctccatat ctcaaaactt
      901 gaccgttggc aatacttgtt ttaaaaatag tttcgtcggt attgaagtag ttgaaaattg
      961 cggtatcata atgagaagaa acgtggaatg ctttggtagc aaacattttt ctgtcttcta
     1021 atgttgtggc accattttga gtactaatca aatccaaaag caaagaatat tcagccaccg
     1081 atggaacgat tactgtgtcc ttgaaatttt tggcagcagc acgaatcaat gaaataccac
     1141 caatatcaat tttttcgata atgtcgcttt cactagctcc agaagccaca gttttttcaa
     1201 aaggatacaa gtccacaatc accaaatcaa tttgaggaat gtcgtattcc ttcatttgtt
     1261 gcacatcgct ttcattgtct tgacggttta agattccacc aaaaactttt ggatgcaaag
     1321 ttttaactct tcctccaaga atggatggat atgatgttac atcttctaca ggaacaaccg
     1381 gaatacctaa gtttttgata aattcttcag ttcctccagt ggaataaaaa gttacatttt
     1441 gctcgtgtaa tttcttaatt attggttcca aaccttcttt tgaaaagaca gaaattaatg
     1501 ctgattggat tttttttgtt gtgctcatcg tgtagttaaa attttgtggt gcaaaagtag
     1561 tttttttgat gataaaattc aaatctattt ctgtaacaat atcttaaaaa aagctaccaa
     1621 tgagttattg atttttcatt aggattatga agaatatttt tggaatttta caataacaca
     1681 aaaccccgtt ttatgctagt ttattttcga ctattatctg agagtttccg atttgcaatg
     1741 aatgctttgc ggaacaataa actgcgtact ttattatcct tgcttggtgt tactatcgga
     1801 attttttcga taattgcagt ccttgctgcg gtcgattcct tagatagaaa gattactaaa
     1861 gatttgagtt ctttagataa aaatactatt tatttgatga aaatgtcatt tggtccttct
     1921 gaaattccac agtggaaaag agaacaattt ccgaatgtaa aatacgatga atatctctat
     1981 ttgaaaggtg caatgaccaa cacagatcaa atgggctacc aaatatttac caaaagcgaa
     2041 tctttaaaat acggttcgaa tacggtaaca tctgtaaatg ttgtgccagt ttcgcacgag
     2101 tttattgaca tacaagggct ggaattcgat gagggcagat tttataccga gtctgaggcc
     2161 aattctgcag ccccagtagt ggtaattgga aatgaaattg cgaagtcttt gtttgaagat
     2221 tctgaaccta ttggaaaaaa aatcagaatg tatggacaac gttttacggt tattggagtt
     2281 ttgaaaaaac aaggctcagg gatgtttggc aatgatgata gttctgcttt tatccctgta
     2341 aattttgttc gtcaacaatt tggggacaat aataaaggat tagtgaatgt aattattttt
     2401 aaacctgaaa aaggagttga tatggacgct tataaagctg aaatctctca aaaactaaga
     2461 gcctttcgcg gtgttaaagc gggtgaaatc gacaattttt tcattaatgt gttttctgga
     2521 ttcaccgatt ttattgacgg aattatttct caaatgaatg ttgtgggttg gattattagc
     2581 ggattttcct tgcttgtggg tggttttggt attgccaata ttatgtttgt ttcagtaaaa
     2641 gaaagaacca atcttatcgg aattcaaaaa tcgctgggag ccaaaaaccg ttttattttg
     2701 tttcaatttt tgtttgaagc cattattcta tcagtaattg gcggaattat tggattgctt
     2761 cttgtttgga ttattgccat gattttgacc aaagcattag atttcgaatt tgttttggga
     2821 atgggaaata tccttttagg aacgggatta gcggccatta ttggactcat ttcgggaatt
     2881 cttccagcta tttctgctgc aaaacttgat cctgttgagg cgattagaac agggatgtaa
     2941 ggaagtgttc agtagtcagt ttttagtgtt cagttgaagc atctctttct tatttttaca
     3001 gtctctttta tttgattgcg tcattttaat tttataaata gcaagaataa aagttaattt
     3061 attactattt ttttcatctt ccaaaatttg aattaaagat ataaaaaaac acaaagcaat
     3121 cactttgtgt ttttttggta tttgtgtgtt tgtaaaaaac tatctaatat cgatattttg
     3181 tatcaaatcg atatacaaat tgatagaatc tttcaattct tttcttggtg taataaaatc
     3241 cagaaaacca tgttccataa caaattcggc agtttgaaaa ccttctggca aatcttttcc
     3301 ggtggtgtct tttacaactc ttggtccagc aaagcctatt aatgctcctg gttctgaaat
     3361 gttgatgtca ccaagcatcg cataagaagc tgttgttcct ccagttgttg ggtcggtaca
     3421 caaagagatg taaggtattt ttgcatcagc taattgggc
//
LOCUS       DAOSNN010000261         3438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_27569_length_3438_cov_5.896246, whole genome shotgun sequence.
ACCESSION   DAOSNN010000261 DAOSNN010000000
VERSION     DAOSNN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27569_length_3438_cov_5.896246"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLQ70_10955"
     CDS             <1..652
                     /locus_tag="PLQ70_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQE35178.1"
                     /translation="PQKNFEDYLGKGFSGDVEGKRAQIKKFTFSKFEFNSPIVAFPDS
                     SSIKNVRMVKNRAGSIGAEILKRFNLIFDYHNQKMFLKKNRNYNLPFNYNKSGIEVQH
                     YGVQWVRETVSQESVTFAGSESAAKSNTIFSDFKYKTVLKPIYIIVHVRKNSPADLAG
                     LKKGDVIVSINQKKGYDFSLEKINSLLKSDEENWIHFEVERENQILKFKFQLLNTL"
     gene            complement(700..1104)
                     /locus_tag="PLQ70_10960"
     CDS             complement(700..1104)
                     /locus_tag="PLQ70_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35179.1"
                     /translation="MNTLQYLKGLTLLLLLTTMVSCTDTDDTNTLIGTWSWTETSGGI
                     AGIHETPASTGKTIDLKFTSNGQYFYYTNGVISSEGTYKFSTQKSIVDGTTKKSIVFS
                     VDREMVIAKIDNENLELDDNVYDGFGSSYIRK"
     gene            complement(1129..1518)
                     /locus_tag="PLQ70_10965"
     CDS             complement(1129..1518)
                     /locus_tag="PLQ70_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HQE35180.1"
                     /translation="MKNIATLIAIVVISSFGFAQNGAKIQFKTTDNTIDYGTISKKND
                     TGIRSIEFTNTGDAPLIISNVLSTSGFTISSKPAASIAPGKTGKFEIKYNMVSGPIRK
                     TITVESNAVNYDSGRIPLKIKGEVIAD"
     gene            1727..>3438
                     /locus_tag="PLQ70_10970"
     CDS             1727..>3438
                     /locus_tag="PLQ70_10970"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQE35181.1"
                     /translation="MTIPAQFDAKTIENKWYDYWMKNNYFHSEPDHRTPYSIVIPPPN
                     VTGVLHMGHMLNNTIQDVLIRRARLKGFNACWVPGTDHASIATEAKVVAKLKSEGINK
                     SDLSREEFLKHAYDWTDKYGGTILEQLKQLGCSCDWDRTKFTMDPDMSASVIKSFVDL
                     YNKGLIYRGYRMVNWDPEAKTTLSDEEVIYEEQNGKLFFLKYKIEGSEDFLTVATTRP
                     ETIFGDTAICINPNDERFSHLKGKKAIVPICGRIIPIIEDEYVDVEFGTGCLKVTPAH
                     DMNDKTLGEKHNLEIIDIFNEDATLNSFGLHYQEKDRFVVRAEIAKELEETGALAKTE
                     IYLNKVGTSERTKAVIEPRLSDQWFLKMEDLVKPAIKSVLEDGDIKLHPARFNNTYAH
                     WLNNIRDWNISRQLWWGQQIPAYFYGDGKEDFVVAENIEDALKLAQAKTHNSQLTIHN
                     LKQDADALDTWFSSWLWPMSVFGGIMDPENEDFKYYYPTNDLVTGPDILFFWVARMII
                     AGYEYAGEKPFSNVYLTGLVRDKQRRKMSKSLGNSPEPLELIEKFGADGVRVGLLLSA
                     SAGN"
BASE COUNT         1131 a          529 c          646 g         1132 t
ORIGIN      
        1 tcctcaaaaa aatttcgagg attatttagg taaaggtttt agtggtgacg ttgaaggaaa
       61 aagagcccag attaagaaat ttactttttc aaaatttgaa tttaattctc caatagtagc
      121 ttttccagat tcgtcttcga ttaaaaatgt gagaatggta aagaatcgag cgggttcaat
      181 cggagcagaa attttaaaaa gattcaattt gatttttgat tatcacaatc aaaagatgtt
      241 tctaaagaaa aatagaaatt ataatttacc tttcaattat aataaaagtg gaatagaagt
      301 gcaacattat ggtgtgcaat gggttagaga aacggtaagt caagaatcgg ttacttttgc
      361 aggaagtgaa tctgctgcaa aaagcaatac tattttcagc gattttaaat ataaaacagt
      421 tttaaaaccc atttatatca ttgtacatgt tcggaaaaat tcaccagcag acttggcagg
      481 actaaaaaaa ggagatgtaa ttgttagtat aaatcaaaag aaaggctatg atttttcttt
      541 agaaaaaatt aattcgttgt tgaaatcgga tgaagaaaac tggatacatt ttgaagtgga
      601 aagggaaaat caaattctaa agtttaaatt tcaattgcta aatactttat aaaagaaacg
      661 cttcttagat tattcttaga agcgttttgt taactagttt tattttctaa tgtaagaact
      721 tccaaaacca tcataaacat tgtcatctaa ttctaaattt tcattatcaa ttttggctat
      781 taccatttcc ctatcaactg aaaacactat tgattttttg gttgtaccat ccacgatact
      841 tttttgagtt gaaaatttat aagttccttc acttgaaata actccattgg tataatagaa
      901 atattggccg ttacttgtga attttaaatc gatggttttt ccagtcgagg caggcgtttc
      961 atgaattccc gcaataccac cactagtttc agtccaactc catgtcccta tcaaagtatt
     1021 agtgtcgtcg gtatctgtgc aagaaaccat tgttgttaat agtaatagaa gcgttaatcc
     1081 ttttagatat tgtagcgtat tcattttttt aaatttcaac taatttactt aatcagcaat
     1141 gacttctcct tttattttca atggaattct tccgctgtca tagtttacag cattcgattc
     1201 aacagtaatg gtttttctaa taggacctga aaccatattg tatttaattt caaatttacc
     1261 agtttttcct ggagcaatag aggctgctgg ttttgatgaa atagtaaaac cagaagtgga
     1321 gagaacattc gaaataatca atggtgcatc accggtatta gtgaattcta tagatcgaat
     1381 tcctgtatca ttttttttcg agatagttcc gtaatctatt gtgttgtccg tggttttgaa
     1441 ttggattttt gcaccattct gagcaaaacc aaaactgctt attactacaa ttgctattag
     1501 tgtggctata tttttcatct tcattttttt agatttggtt tagtaaaaat actttttttt
     1561 aattaagagt acaattattt atttcgcttt ttatactgta ctttattatt tacttttgtc
     1621 ctcaatttaa attacaaaaa tcagaccaaa atagaggttt atttgtttaa tcggttattt
     1681 cgcccaatcg actaaacaaa taaccgatta acaaataaat acaacaatga caattcctgc
     1741 acaatttgac gctaaaacca ttgaaaataa gtggtatgac tactggatga agaacaatta
     1801 ttttcattcg gagcctgacc atagaactcc atattctatt gtaattccgc caccaaatgt
     1861 tactggagtt ttgcatatgg gacatatgtt gaacaacacg attcaagatg ttttgattcg
     1921 tcgtgctcgt ttaaaagggt tcaacgcttg ttgggttcct ggaacggatc acgcttcgat
     1981 tgctacggaa gctaaagtag tggccaaatt aaaatcggaa ggaattaata aatcggattt
     2041 gagtcgggaa gagtttttga aacacgctta cgattggaca gacaaatacg gaggaaccat
     2101 cttggaacaa ttgaaacaat tgggttgctc ttgcgattgg gatagaacca aattcacgat
     2161 ggatcctgat atgtcagctt ctgttattaa atctttcgtt gatttgtaca acaaaggatt
     2221 aatttatcgt ggttatcgaa tggtaaactg ggatccagaa gccaaaacga ctttgtctga
     2281 cgaagaagtt atttatgaag aacaaaacgg aaaattattt ttcctaaaat ataaaatcga
     2341 aggttctgaa gactttttga cagtagcaac aacgcgtcca gaaactattt ttggagatac
     2401 agctatttgt attaatccaa atgacgaacg tttttctcat ttgaaaggga agaaagcgat
     2461 tgtgccaatt tgtggaagaa ttattccaat tatcgaagac gaatatgttg atgtagagtt
     2521 cggaacaggt tgcttaaaag tgacaccggc tcacgatatg aatgacaaaa cgctaggtga
     2581 gaaacacaat ctagaaatca tcgatatttt caatgaagat gccactttga acagttttgg
     2641 tttgcattat caagagaaag atcgttttgt ggttcgtgcc gaaattgcaa aagaattaga
     2701 agaaactggc gctttggcaa aaactgaaat ctacctgaat aaagtgggaa cttctgaaag
     2761 aaccaaagcc gtaatcgaac ctcgtttgtc cgatcaatgg tttttgaaaa tggaagattt
     2821 ggtaaaacca gccatcaaat ctgttttgga agatggcgat atcaaattgc atccagcacg
     2881 ttttaataat acctatgcgc attggttgaa caatattcgc gattggaata tttctcgtca
     2941 gttgtggtgg ggacaacaaa ttcctgctta tttctacggc gacggaaaag aagatttcgt
     3001 agttgccgaa aacattgaag atgccttaaa attagcgcaa gccaaaactc acaattcaca
     3061 actcacaatt cacaatttaa agcaagatgc cgatgcgtta gatacttggt tttcttcttg
     3121 gttgtggcca atgtcggttt ttggtggaat tatggatcct gaaaacgaag attttaaata
     3181 ctattatcca acgaatgatt tagttacggg tccagatatt ttatttttct gggtagcgcg
     3241 aatgattatt gcgggttatg aatatgcggg cgaaaaacca ttttcgaatg tgtatttgac
     3301 tggtttggtt cgtgacaaac aaagacgtaa aatgtctaaa tcattaggaa attctccgga
     3361 gcctttggaa ttaatcgaaa aatttggtgc agatggtgtt cgtgtagggt tgcttttgag
     3421 cgcttcagca ggaaacga
//
LOCUS       DAOSNN010000262         3420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_27747_length_3420_cov_4.399108, whole genome shotgun sequence.
ACCESSION   DAOSNN010000262 DAOSNN010000000
VERSION     DAOSNN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27747_length_3420_cov_4.399108"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="PLQ70_10975"
     CDS             <1..1322
                     /locus_tag="PLQ70_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HQE35182.1"
                     /translation="PKRAITMGVSTILRSKRIVLMAWGQNKASIIKRTIQGEISSEVP
                     ATFLQNHNNATFVLDQSAASELTRFETPWLVGECIWTKELKHKAIVWLCKETNQAILK
                     LTDRDYNNNGMSDLLAQEGSAYDLNINMFNVLQHTITGWPGGKPNVDDSHRPERANPA
                     QKRVILFSPHPDDDVISMGGTFSKLIKQGHDVHVVYQTSGNIAVTDDEALKFAEVCND
                     FIGGKKDLDINFQEVIDFINNKKENEVDSSEVRKLKGLIRRRESYAATRYIGLKDENT
                     HFLDLPFYETGKIKKNPLGAEDIAIVKDIIEKIKPHQVFAAGDLADPHGTHEVCLNAI
                     LLAMNLLKHESFMNDCWLWLYRGAWHEWDVHEIDMAVPLSPDEVLLKRHAILYHQSQK
                     DRVMFQGNDSREFWVRAEDRNKNTAKIYDELGLSEYQAIEAFKRYNY"
     gene            1602..>3420
                     /locus_tag="PLQ70_10980"
     CDS             1602..>3420
                     /locus_tag="PLQ70_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQE35183.1"
                     /translation="MAFSNLFRKKTVHDILKQVDKNESDGHHVLGKHLNARDLTAFGI
                     AAIVGAGIFSTIGKASADGGPAVIFLFLFTAIACSFAAFAYAEFASMVPVSGSAYTYS
                     YVAFGEIIAWIIGWALIMEYAIGNITVAISWSDYFTGLLESGGIKLPQWIQMDYLTAS
                     NGFKDATALMHGGKSFENLSAGLQASYTAWTTSPVIGSFHFVADLPALLIIVLITALV
                     YRGMKESRNASNLMVVIKLCIVLLVIAVGVFYVNTDNWHPFAPNGVSGVLKGVSAVFF
                     AYIGFDAISTTAEECKNPQRDLPRGMMWAIIICTLLYIAIALVLTGMVSYNELSVGDP
                     LAFVFDKLHIGWMSGIIGVSAVIAMASVLLVFQMGQPRIWMSMSRDGLLPKRFSTVHP
                     KFKTPSFATIVTGFVVAVPALFLNLTMVTDLCSIGTLFAFVLVCAGVLVLQNKKDIPR
                     GKFKTPYVNSKYVIPFLLIIALVFAFKYNNKATMAFLTNEKQISSPSVIVTSLNKDKS
                     KEVYEFIAQNYGDKKIASEDLEQVLSSFSQDDVLYKTVVDSLPIDISLKYESGFSLFK
                     HKIPMWIFLIVMIGLTVWSNRSNLSLIPLLGLICCLYMMA"
BASE COUNT         1082 a          487 c          695 g         1156 t
ORIGIN      
        1 ttcctaaaag agctattaca atgggagttt ccactattct cagatcaaaa agaattgtat
       61 taatggcttg gggacaaaat aaggcttcca tcattaagag aaccattcaa ggagaaataa
      121 gctccgaagt tccagctact tttttgcaaa atcataataa cgcaactttc gttttagacc
      181 aatcggcagc ttcagaatta acccgatttg aaacaccatg gttagttgga gaatgtattt
      241 ggacaaaaga attgaaacac aaagctattg tttggctgtg taaagaaacc aatcaagcca
      301 tattaaaact taccgataga gattacaata ataatggaat gtcagacctt ttggcacaag
      361 aaggttctgc ttatgatttg aatatcaata tgttcaatgt tttgcagcat actataacgg
      421 gttggccagg cggaaaacca aatgttgatg attctcatag gccagaaaga gccaatcctg
      481 cccaaaaaag agttattctt tttagtccac atccagatga tgatgtaatt tctatggggg
      541 gaactttttc taaattgatt aagcaaggac acgatgtgca tgttgtttat caaacctcag
      601 gaaacatagc tgttactgat gatgaagctt tgaaatttgc cgaagtgtgc aatgatttta
      661 ttggaggcaa aaaagatttg gatattaatt ttcaagaggt tattgatttc attaataata
      721 aaaaagaaaa tgaagtcgat tcttcggaag ttagaaaatt aaagggatta atcagacgtc
      781 gagaatctta tgcagccaca cgttacattg gattaaaaga tgaaaacacc cattttttgg
      841 atcttccttt ttatgaaaca ggaaaaataa agaaaaatcc attgggagca gaagatattg
      901 ctatagtaaa agacattata gaaaaaatca aaccacatca ggtttttgct gcaggcgatt
      961 tagccgatcc acacggtact catgaagttt gtctaaatgc cattttgttg gcaatgaatt
     1021 tattgaaaca cgaatctttt atgaatgatt gttggttgtg gttgtataga ggagcttggc
     1081 acgaatggga tgttcatgaa atagatatgg cggttccatt aagtcctgac gaggttttgt
     1141 taaaaagaca tgctattttg tatcaccaat ctcaaaaaga cagagtaatg tttcaaggca
     1201 acgattcaag agaattttgg gtaagagcag aggatcgcaa caagaatact gcaaaaattt
     1261 atgatgagct agggttgtcc gaatatcagg cgattgaagc cttcaaacgc tataattact
     1321 agtaaaaagc aattcaaaag tcagattata aaagactaat tcaaatattg aacttttaga
     1381 aaaagtggtt ggaagaaatt tcgaccactt tttttagttt tattcaactt aaaaaaaaag
     1441 aaatagattt taaaataaag aaggacattt taagaacaat tggaagttaa aaatagcaac
     1501 tcaaaacttt ataaattgaa tttgttttct atatttgaaa atataaaata gggaactaaa
     1561 atagatttta aattagtagc ccataactat aaaattcacc aatggcattt tcaaatttat
     1621 ttagaaaaaa aacagttcac gatattttaa agcaagtaga taaaaacgag tctgatggac
     1681 atcatgtttt aggaaaacac ttaaacgctc gtgatttgac tgcgtttgga attgcagcaa
     1741 ttgttggagc aggaattttt agtaccatcg gaaaagctag tgccgatggt ggtccagccg
     1801 ttattttcct gtttttattt actgcaattg cttgtagttt tgcggctttt gcttatgccg
     1861 aatttgcgtc aatggttccg gtttctggga gtgcttatac ttattcttat gtggcttttg
     1921 gagaaattat tgcttggatt attggttggg ctttgattat ggagtatgct attggcaata
     1981 taacggttgc catttcgtgg agtgattatt ttacaggctt gctcgaaagt ggtggaatta
     2041 aacttccgca atggattcag atggattatt taacagcttc gaatggcttt aaagatgcaa
     2101 cagcattgat gcacggaggg aaatctttcg aaaatttaag tgctggttta caagcttcct
     2161 atacggcttg gacgacttcg cctgtaattg gttcttttca ttttgttgct gatttacctg
     2221 cattattaat tatagtttta attacggctt tggtttatcg tggaatgaaa gagtccagaa
     2281 atgctagtaa tttgatggta gtaattaaat tatgcattgt acttttagtg attgctgttg
     2341 gtgtttttta tgtaaatact gataattggc atccgtttgc acccaatgga gtttcaggag
     2401 ttttaaaagg agtttcggct gttttctttg cttatattgg ttttgatgct atttcaacta
     2461 ctgccgaaga gtgcaaaaat ccacagcgtg atttgcctcg aggaatgatg tgggcaataa
     2521 ttatttgtac ccttttatat attgccatag ccttagtgtt gacaggaatg gtcagttata
     2581 atgaattgag tgtgggcgat ccgttagctt ttgtttttga taaattacac atcggatgga
     2641 tgtcaggaat tattggtgta agcgccgtaa tagcaatggc gagcgttctt ttagtttttc
     2701 agatggggca acctcgaatt tggatgagta tgagccgtga cggattgttg cctaaacgtt
     2761 tttctacagt gcatccaaag ttcaaaacac cttcgtttgc caccattgtt actggatttg
     2821 ttgtagcagt tccagctttg tttttgaatt tgacaatggt aaccgatttg tgtagtattg
     2881 gaaccttgtt tgcctttgtt ttggtttgtg ctggggtttt ggttttgcaa aataaaaagg
     2941 atattcctcg aggaaaattc aaaacacctt atgttaattc aaaatatgtc attccttttt
     3001 tgctaattat cgctttggtt tttgccttta aatacaacaa taaagcaaca atggcttttt
     3061 tgaccaatga aaaacaaatt agttcgccat cagttattgt gacttcctta aataaggata
     3121 aatctaaaga agtttatgaa tttattgctc aaaattatgg agataaaaaa attgcttccg
     3181 aagatttaga gcaagttttg agtagtttta gtcaggatga tgtgctttat aaaacggttg
     3241 tagattcatt gccaatagac atttctctga aatatgaaag tggttttagc ctttttaaac
     3301 ataaaattcc aatgtggatt ttcctgattg ttatgatagg tctcacagtt tggtctaata
     3361 gatctaattt gtctttaatt ccattattgg gattgatttg ttgtttgtat atgatggcag
//
LOCUS       DAOSNN010000263         3406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_27899_length_3406_cov_4.139063, whole genome shotgun sequence.
ACCESSION   DAOSNN010000263 DAOSNN010000000
VERSION     DAOSNN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27899_length_3406_cov_4.139063"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLQ70_10985"
     CDS             <1..223
                     /locus_tag="PLQ70_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HQE35184.1"
                     /translation="SYIYLNSQYTFLNSLTYEQRISELSLNPDRSDVIIPATQIYLNA
                     MKWSGARNIYVPKIGLSDGIVKAMYYGKI"
     gene            496..>3406
                     /gene="ccsA"
                     /locus_tag="PLQ70_10990"
     CDS             496..>3406
                     /gene="ccsA"
                     /locus_tag="PLQ70_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQE35185.1"
                     /translation="MDKKILSILFSTRLMAILFLTFALAMGIGTFIESKYNTDTARIW
                     IYNAWWFEGIMLVFMINFIGNIKRYKLLRKEKWATLMLHLSFIFIIAGAFITRYISYE
                     GIMPIREGNTENIFYSDKMYLTVFVDGEYQGGMKRRVFEKPLLLSPVTNNNFSISGDF
                     ADIPFEVNYENFILGAKEVIKPDPKGSLYFKLVEAGQSGREEHFLKEGEVQNIHNTLF
                     ALNKPTKGAINITLNGSEATIQTPFEGEFMRMADKLQGKVAKDIVQPLMMRSLYSIGD
                     MRVVFPEPAIKGTIGYDSKNDYKAKEHNDALTLKLKAEGQEKTITLIGTKGLVGQSKS
                     VKIGKIDYTFTYGNKVYNLPFSIKLNDFIAQKYPGTERSFSSFESQVTVTNKSESFDA
                     RIFMNNILDYEGYRFFQSSFDPDEMGTVLSVSHDYWGTMITYIGYFMLYFALMAIMFT
                     KHSRFADLKRKLEVVKTKKANLLPLLLLFLSFGAFAQNHNNAQFLSEKQLDSIIEKRK
                     VTEDHAAKFGRLVVQDAGGRMKPINTFSSELLRKVSHKNDYKGMNSDQVFLSMTQGGN
                     IWIQVPLIYIKAGNDSIRKIIGIDAEKPFASFIDFFDNKGNYKLSPYLDAAYKAANPN
                     QFEKDFIETDKKVNLMESALSGSILKIFPIPNDPNNKWVSYPELINSGMKGMAATYTQ
                     NILQMYFTALEEASVSNDYKKADGLLESINGFQKKYGSKVLPSDQKISSEILYNKYDI
                     FQKLPYWYMYAAILMLLFTILKIFKERKFLDYAINTMHIIVGLLFALHTFALIARWYI
                     SGHAPWSNAYESIIYVAWATMFFGLAFDRKSKLTVASSAFVTAMILTAAYMNWIDPEI
                     GNLQPVLNSYWLMIHVAIIVASYGPFALGMILGVVSLLLILFTTEKNKDKMLLNINEI
                     SYINEMALTIGLIMLTIGNFLGGQWANESWGRYWGWDPKETWALISIMVYAFVIHA"
BASE COUNT         1132 a          542 c          605 g         1127 t
ORIGIN      
        1 gtcttatatt tacttgaact cacaatatac attcttgaat tcattgacat acgaacaaag
       61 aatttctgaa ctgagtttga atcctgaccg ttctgacgta attattcctg caacacaaat
      121 ctatcttaat gcaatgaaat ggagtggtgc cagaaatatt tatgtgccta aaattggtct
      181 ttcagacgga attgtaaaag caatgtatta tgggaaaatt tgatttttat aacagtatct
      241 aaaattttca agggggataa aaataatttt caatagttat tttattccct tttttattcc
      301 ctcgttaatg aaattgaatc tgtaaaacaa ttaatcccac agtaactttt gctagccaga
      361 taactgaaaa ttaaatttaa gaaatgaaac tgattcttag cttaaatttt gattaaatta
      421 acaatctagg gaagagaaaa ttcaacaatt ataagtactt ttgtagtacc ttttataaaa
      481 cgtaatttct tacagatgga taaaaaaatt ctttctattt tgttttcaac acgattaatg
      541 gcaatacttt tcttgacttt tgcacttgca atgggaatag ggactttcat cgaaagcaaa
      601 tacaacaccg atacagcccg aatttggatt tataatgcct ggtggttcga aggaattatg
      661 ttggttttta tgatcaattt cattggtaac atcaaacgat ataaactact aagaaaagaa
      721 aaatgggcta cactgatgct tcatttatct tttattttta ttatagctgg agcttttatt
      781 actcgttata tcagttatga aggaataatg cctattcgtg aagggaatac cgaaaatata
      841 ttctattccg ataaaatgta ccttactgtt tttgtcgatg gtgagtatca gggaggtatg
      901 aaacgccggg tttttgaaaa accattacta ctttcgcccg taaccaataa taatttttct
      961 atttcaggtg attttgccga cattccattc gaagtcaatt acgaaaattt cattttagga
     1021 gccaaagagg tgattaaacc cgatccaaaa ggaagtttgt attttaaact ggttgaagca
     1081 ggccaaagtg gtcgcgaaga acatttcttg aaagaaggcg aagttcaaaa cattcacaac
     1141 acactttttg ctttaaataa accaacaaaa ggagccataa atattacttt aaacggtagt
     1201 gaagcaacta ttcaaactcc atttgaaggc gagtttatgc gaatggccga caaactacaa
     1261 ggcaaagttg ccaaagatat tgttcagcct ttaatgatgc gttctttgta ttctattggc
     1321 gatatgagag tggtttttcc agaaccagct atcaaaggaa ctattggtta cgattcaaaa
     1381 aatgattata aggctaaaga acacaacgat gcccttactc taaaattaaa agccgaaggt
     1441 caagaaaaaa caataacatt aatcggtaca aaaggacttg ttggacaatc caaatctgtg
     1501 aaaataggga agattgatta tacttttact tatggaaaca aagtctataa tttgcctttt
     1561 agtattaaat tgaatgattt tattgcccaa aaatatcctg gaaccgaaag aagtttttct
     1621 tctttcgaaa gccaagttac cgttacaaat aaaagtgaaa gttttgatgc tagaatcttt
     1681 atgaataaca ttttagatta tgaaggctat cgcttttttc aatcctcatt cgaccccgat
     1741 gaaatgggaa ctgttttatc agtaagccac gattattggg gaactatgat tacttatatt
     1801 ggttatttta tgttgtattt tgctttaatg gccattatgt ttaccaaaca ttctcgcttt
     1861 gccgatttaa aacgaaaact agaagtggtg aaaactaaaa aggctaattt gctaccctta
     1921 ttgttgctgt ttttgagttt tggagctttt gctcaaaacc ataataatgc tcaattttta
     1981 tctgaaaaac agttagattc tatcatcgaa aaacgcaaag ttactgagga tcacgctgcc
     2041 aaatttggaa gattagttgt tcaggatgca ggcggtagaa tgaaacctat aaacactttc
     2101 tcctctgaat tattgcgaaa agtaagtcat aaaaatgact acaaaggaat gaattccgac
     2161 caagtctttt tgtctatgac tcaaggcgga aacatttgga ttcaagttcc tttgatttat
     2221 atcaaagccg gaaacgacag tattcgtaag attattggaa ttgatgccga aaagccgttc
     2281 gcttcgttta ttgacttttt tgacaataag ggaaattata aattatcacc ttatttagat
     2341 gcagcataca aagcagcaaa tccaaaccaa tttgaaaaag attttatcga aacggataag
     2401 aaagtaaact taatggaatc ggcactgagc ggtagtattt tgaaaatttt tccaattcca
     2461 aacgatccta ataataaatg ggtttcctat cccgaattga taaattctgg tatgaaagga
     2521 atggctgcaa cttatactca aaatattctt caaatgtatt ttaccgcttt agaagaagca
     2581 tctgtttcta acgattataa aaaagccgat gggctgctag aaagtatcaa tggattccaa
     2641 aaaaaatacg gtagcaaagt attgccatcc gaccaaaaaa ttagttcgga aatcttgtat
     2701 aacaaatatg atatttttca aaagttgcct tattggtata tgtatgccgc aatattgatg
     2761 cttcttttca ccattttaaa aatatttaaa gaaagaaaat ttctcgatta tgctattaat
     2821 acaatgcaca ttattgttgg gttacttttt gccttacata cttttgcttt aattgctcgt
     2881 tggtacattt ctggtcatgc accttggagt aacgcttatg aatccatcat ttatgttgct
     2941 tgggcgacca tgttttttgg tttggctttt gaccgcaaat cgaaactaac agtggcttca
     3001 tctgcttttg tcacagccat gattttgacg gctgcttata tgaactggat tgatcctgaa
     3061 attggcaatt tgcaacctgt attaaattct tattggttaa tgattcacgt ggcgataatc
     3121 gtggcaagtt acggaccatt tgccttggga atgattctag gagtggtttc attgctgttg
     3181 attttgttta ccactgaaaa gaataaagac aaaatgcttc taaatatcaa tgaaatatca
     3241 tacatcaacg aaatggcact caccatagga ttgattatgt tgaccatcgg aaattttctt
     3301 ggcggtcaat gggccaacga aagttgggga cgttattggg gatgggaccc taaagaaact
     3361 tgggctttga ttagtataat ggtctatgct tttgtgattc atgccc
//
LOCUS       DAOSNN010000264         3378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_28192_length_3378_cov_4.205236, whole genome shotgun sequence.
ACCESSION   DAOSNN010000264 DAOSNN010000000
VERSION     DAOSNN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28192_length_3378_cov_4.205236"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1263)
                     /locus_tag="PLQ70_10995"
     CDS             complement(22..1263)
                     /locus_tag="PLQ70_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQE35186.1"
                     /translation="MFKLFRENVRIAFGSIRTQLLRTILTVLIIAIGIMALVVILTVI
                     AALENKLTSNFASMGANTFNINQYENTSRRRGGEEREIINPIISYPEAVAFKNKYKFP
                     LTETSISFTATSTAEIKFESNKTDPENKIVGVDEFFMANSGLETSSGRNFTGFDIQNN
                     TYSCIVGSDFEKGLLKDVNPIDKIISIRGAKFKVIGVLKEKGSTFGNSQDLRVLIPIQ
                     LARSLFTAPNINYTVSAMVSKNELLEQAVDNAIITMRKVRKLSPVKDNNFAVSRSDDL
                     IHRIEDMTGYLKIAAFVIGLITILGSSIALMNIMIVSVTERTREIGVRKALGAKKSTI
                     AFQFFIETLLIGQLGGLVGVLLGISIGNLIALAMEFDFVIPWDAIIVAFGISFIVAVV
                     SGSYPAVKASKLDPIEALRYE"
     gene            1408..2778
                     /gene="hisS"
                     /locus_tag="PLQ70_11000"
     CDS             1408..2778
                     /gene="hisS"
                     /locus_tag="PLQ70_11000"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008256711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQE35187.1"
                     /translation="MASKPSIPKGTRDFSPAEVAKRQYIIATIKSNFEKFGFQPIETP
                     SFENSETLMGKYGEEGDRLIFKILNSGDYLAKADAALLQNKESNKLTSSISEKALRYD
                     LTVPFARYVVQHQSEIEFPFKRYQIQPVWRADRPQKGRFREFFQCDADVVGSKSLWQE
                     VELVQLYDSVFTQLGLEGVTIKINNRKILSGIAEVIGAKDKLIDFTVALDKLDKIGED
                     GVKKEMIEKGIAESAIEKVQPLFNFTGTISEKIEKLSALLVESEEGLKGVEELRFICD
                     NVTNLGLNKAVLDLDVTLARGLNYYTGAIFEVAAPKTVSMGSIGGGGRYDDLTGIFGL
                     KDMSGVGISFGLDRIYLVIEELNLFPKTVTSTSKALFINYGEKEAFYSMNAITKLRSF
                     GVKVELYPDNVKVGKQFQHADKRGIPFAVIVGESEMNEGKFALKNLFSGEQNLLDFEG
                     LKAVLE"
     gene            complement(2876..3046)
                     /locus_tag="PLQ70_11005"
     CDS             complement(2876..3046)
                     /locus_tag="PLQ70_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35188.1"
                     /translation="MKKLFTVLAIALFTITISAQQTKPATSTKKEATAKKCTAEEKAK
                     CEKTTKSCCSKK"
     gene            complement(3199..>3378)
                     /locus_tag="PLQ70_11010"
     CDS             complement(3199..>3378)
                     /locus_tag="PLQ70_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQE35189.1"
                     /translation="NLVEKVTLYSINGQSIANWKIENQDQQNIQISINTISSGIYIAK
                     LKTTTGELSKKIIIN"
BASE COUNT         1098 a          498 c          643 g         1139 t
ORIGIN      
        1 taaatatcgt aaatcgttta tttattcata tctcaacgcc tcaatcgggt ctagtttaga
       61 agctttaacg gcaggatatg aacctgaaac tactgcgaca atgaaactga tgccaaaggc
      121 aacaattatg gcatcccaag gaattacaaa atcaaattcc attgctaatg cgataaggtt
      181 tccaatcgaa atacctagta aaactccaac aagaccacct aattgaccaa ttagcaacgt
      241 ttctatgaaa aactggaagg caatagtgct ttttttggct cccaatgctt tacgaacacc
      301 aatttctcgt gtgcgttcgg taaccgaaac aatcataata ttcattaaag ctatagagga
      361 acctaaaatg gtaatcagtc caattacaaa ggcggcaatt tttaaataac ctgtcatatc
      421 ttcaattcta tgaatcaaat catcacttct tgaaacagca aaattattgt cttttacggg
      481 acttaatttt cgaactttcc tcattgtaat tattgcatta tcaacagctt gctctaataa
      541 ttcgttttta cttaccatcg cactcacggt gtaatttata ttaggtgcgg taaacaagga
      601 acgagccagt tgaataggaa ttaaaactct taaatcctgg ctgttgccaa aggtagagcc
      661 tttttctttt aatactccaa tcactttgaa tttggctcca cgaattgaaa ttattttatc
      721 tatagggttg acatctttta gcaatccttt ttcaaaatca gatccaacga tgcaagaata
      781 agtgttgttt tggatgtcaa atccagtaaa atttcggcct gaactggttt ctaaacctga
      841 gtttgccata aaaaattcat ctacacctac aattttattt tcgggatcgg ttttgttgga
      901 ttcaaatttt atctcagcag tagaagttgc tgtaaatgaa atagaggttt cggtcaaggg
      961 aaatttgtac ttgtttttga aagccactgc ttcagggtaa gaaatgatgg gatttataat
     1021 ctctctttct tcgccacctc ttcttcggga tgtgttttca tattgattga tgttgaaagt
     1081 attagcgccc atcgaagcaa agttggaggt caatttattt tctaaagcag caattaccgt
     1141 caaaataact actaaagcca taattccaat agcgataatc aacacggtta ggatggtacg
     1201 caacaattgg gttcggatag aaccaaaagc aattcggacg ttttctctaa ataatttaaa
     1261 catagtgctt atttgacacg aaattacaat ttttgttaca agtttgtttt agaagatggg
     1321 atatttattt taaaaacaag atatttgcag tgagaattaa taattgacaa ttgttaattc
     1381 ttaattgata attcgtaatt cattaaaatg gcatcaaaac caagcattcc aaaaggaacc
     1441 agagattttt caccagctga ggtggcaaaa cgtcaatata ttatagcaac tataaaaagt
     1501 aatttcgaga aatttggatt tcaacccata gaaacaccct cgttcgaaaa ctccgaaacc
     1561 ctgatgggaa aatatgggga agaaggcgat cgattgattt ttaagatttt gaattctggt
     1621 gattatttgg cgaaagccga tgctgcatta ttgcaaaata aagaaagcaa taaactgact
     1681 tctagtattt ctgagaaagc acttcgctat gacttaaccg ttccgtttgc tagatatgtg
     1741 gtgcaacacc aaagcgaaat cgaatttcca ttcaaaagat accaaattca accggtttgg
     1801 cgtgctgatc gtccgcaaaa aggacgtttc agagaatttt ttcaatgtga tgccgatgtt
     1861 gtgggttcaa aatccttgtg gcaagaagta gaattggtgc aattgtacga ttcggttttt
     1921 acacaattag gtttggaagg cgtgacgata aaaatcaata accgaaaaat cctttcagga
     1981 attgccgaag tgattggtgc caaagataaa ttaattgatt ttacggtggc tttggacaaa
     2041 ctcgacaaaa taggtgagga tggtgtaaaa aaggaaatga tcgaaaaagg aattgcggaa
     2101 tctgcaatcg aaaaagtgca accgttattc aatttcacag gaacaatttc tgagaaaatc
     2161 gagaaacttt cggctttgtt ggtagaatct gaagaaggat tgaaaggagt agaagagttg
     2221 cgatttattt gtgacaacgt gactaacctt ggtttgaata aagcggtttt agatttagat
     2281 gtaactttag ctcgtggatt gaattattat acaggagcta tttttgaagt agccgcacca
     2341 aaaacagttt cgatgggttc tatcggaggt ggtggaagat atgatgattt gactgggatt
     2401 ttcggtttga aagatatgag tggagtaggg atttcttttg gtttagaccg aatttatctg
     2461 gttatcgaag aattgaattt atttccaaaa acggtgactt ctacttcaaa agctttgttt
     2521 attaattatg gtgaaaaaga agctttttat tcgatgaatg ccataacaaa attgagaagt
     2581 tttggagtaa aagtagaatt atatcctgat aatgtgaagg taggaaaaca gtttcaacat
     2641 gctgataaac gtggaattcc gtttgccgtt atcgttggag aatcagaaat gaatgaggga
     2701 aaatttgcct tgaagaattt attttcagga gagcaaaatt tgcttgattt tgagggtttg
     2761 aaagcggttt tggagtaatt tagcttttga tttttgaatg aagatttgaa aatagaatta
     2821 cataaaaaaa tgcctcgaaa tttcgaggca ttttttgtaa atataaaagt ggttgttatt
     2881 tttttgaaca acagctttta gttgtttttt cgcattttgc tttttcttcg gcggtgcatt
     2941 tttttgcagt tgcttctttt ttagtagaag ttgctggttt tgtttgttgt gcactaattg
     3001 taatagtgaa taaagctatt gctaatacgg taaataattt tttcatttta atattgaatt
     3061 aattgttatt aactaagtat agtgttcaaa atcagtagca aatatagtaa gaaaaaagca
     3121 tataaaacta ctaagcaagt taatgtttcc ttaataaatc gcatttttga tttggctgat
     3181 ttttccaaaa taattagatt aattaatgat aattttttta ctcaattctc cagttgttgt
     3241 ttttagtttt gctatgtaaa ttcctgaact aattgtgtta attgaaattt gaatgttttg
     3301 ttggtcttga ttttcaattt tccaattagc tatagattga ccgttgatgc tatataatgt
     3361 gactttttca actaagtt
//
LOCUS       DAOSNN010000265         3373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_28244_length_3373_cov_4.959011, whole genome shotgun sequence.
ACCESSION   DAOSNN010000265 DAOSNN010000000
VERSION     DAOSNN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28244_length_3373_cov_4.959011"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PLQ70_11015"
     CDS             <1..579
                     /locus_tag="PLQ70_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HQE35190.1"
                     /translation="NTQAVFSLRGKPLNSYGMSKKIVYENEEFNLLQAALDIEDDMEN
                     LRYNNIVIATDADVDGMHIRLLLITFFLQFFPELIKEGHLYILQTPLFRVRNKKETIY
                     CYSEEERKDAIEKLKPKPEITRFKGLGEISPDEFKNFIGDTIRLDPIMMDKNTSIEQL
                     LSFYMGKNTPDRQDFIIKNLKVELDTLEEIKI"
     gene            597..3323
                     /locus_tag="PLQ70_11020"
     CDS             597..3323
                     /locus_tag="PLQ70_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HQE35191.1"
                     /translation="MKDEEDDNIIPNEENNSEENPIDGNQEDSDADGIIKVEAKHFEG
                     GHFYENDENAEDTITKVTGMYKDWFLDYASYVILERAVPAIEDGFKPVQRRIMHSLKE
                     LDDGRYNKVANVVGHTMQYHPHGDQSIGDAMVQIGQKELLIDCQGNWGNILTGDGAAA
                     SRYIEARLSKFALEVLYSPKITDWGVSYDGRRAEPNNLPVKFPLLLAQGAEGIAVGLS
                     TKVLPHNFNELIDASIKILKGKSFTLYPDFMTAGIADVSNYNDGMRGGRVRVRAKIAQ
                     LDKNTLVITQIPFSTNTTTLIDSILKANEKGKIKIKKIEDNTAAEVEILIHLYPGVSP
                     DKTIDALFAFTACETSVAPLGCVIEDNKPLFVGVSDMLKISTERTVDLLRQELEIQLE
                     ELKNKWHFSTLEKIFIREEMYIDFKLYSDRESLYKYMYDRFEPFKKSFVREINDDDLQ
                     RLTQIPMIRITRFDSDKADDLIAKLEDEMKEVEHNLANLTDFAIAYFTKLKEKYGKGR
                     ERQTELRSFDNIEATKVALRNTKLYVNREEGFIGTGLKKDEYVTDCSDIDDVIVFLRD
                     GNMMICKVDDKKFVGKDIIHVAVFDKGDKRTIYNMIYRDGKSGPSYIKRFNVSGVTRD
                     KLYDLTNETKGSQILYFTCNPNGEAEVITIILRQIGSIKKLKWDVDFANTAIKGRASK
                     GNLVSKYPIKKIEIKEKGISTLKPRKIWFDDTVQKLNVDARGELLGEFRPNDKILVIS
                     QTGKLKVIIPELATHFDEDMVVLEKWHPKKPVSAIYYDGEKERYYIKRFLIENENKEE
                     TFITEHPKSQLEIVSTDYRPVAELIFAKVKGVQKENQTVDIESFIAVKGLKALGNQLT
                     TEKLKQVNLLEPLPYEEPEEVVAEDLEVQGNTEIEEGEIQLDDDGQITLSLE"
BASE COUNT         1155 a          549 c          723 g          946 t
ORIGIN      
        1 aatacacaag ccgtttttag tttgcgtgga aagcctttga attcgtatgg aatgagcaag
       61 aaaattgttt atgaaaacga agaattcaac ctcttgcaag cggctttaga tatcgaagac
      121 gatatggaaa acttgcgtta caacaacatt gtaatcgcta ccgatgctga tgtcgacgga
      181 atgcacattc gattgttgtt gattacattc ttcctgcaat tttttccgga attaatcaag
      241 gaaggacatt tgtatatttt gcaaacacca cttttcaggg ttcgaaacaa gaaagaaacc
      301 atttattgct attcggaaga agagcgtaaa gatgccatcg aaaaattgaa accaaaacca
      361 gaaatcaccc gatttaaagg tttaggagag atttcgccag acgaattcaa gaattttatt
      421 ggtgatacta ttcgattaga ccccattatg atggacaaaa acacttcaat cgagcaatta
      481 ttgtctttct atatgggtaa aaacacgcca gacagacaag attttattat caagaatttg
      541 aaagtggaat tagacacttt agaagaaatt aaaatctaaa aaagatttca aaataaatga
      601 aagacgaaga agacgataac atcattccaa acgaagaaaa taattcggaa gagaatccca
      661 ttgatggaaa tcaagaagat agcgatgccg atggaattat aaaagtggaa gccaaacatt
      721 ttgaaggcgg acatttctat gagaacgacg aaaacgcaga agacaccatt accaaggtta
      781 ccggaatgta caaagactgg tttttggact atgcttcgta tgtaattttg gagcgtgccg
      841 ttccggctat tgaggacggt ttcaaacccg ttcagcgtag aattatgcac tcgctcaaag
      901 aattggatga cggtcgttac aacaaagtgg cgaacgttgt tggacacacg atgcaatacc
      961 atccgcacgg agatcagagt attggagatg cgatggtgca aattggccaa aaagaattgc
     1021 tgatcgattg ccaaggaaac tggggaaata tcctcacggg cgatggagca gcggcttctc
     1081 gttatatcga agcccgtttg tcaaaatttg ctttggaagt tttgtattcg ccaaaaatta
     1141 ccgattgggg cgtttcctat gacggtcgtc gtgccgaacc gaataatctt ccggtgaagt
     1201 ttccgttgct tttggcacaa ggagctgaag ggatagcggt tggtctttca actaaagtct
     1261 taccccataa tttcaacgaa ttaattgacg cttcgattaa gattctgaaa gggaaatcct
     1321 ttacactcta tcctgatttt atgacagcgg gaattgccga tgtgtctaat tacaatgacg
     1381 gaatgcgtgg cggacgagtt cgtgttcgtg ccaaaattgc gcagttggac aaaaatactt
     1441 tggtgattac ccaaattccg ttttcaacca acaccacgac tttgattgac agtattttga
     1501 aggccaacga aaaaggtaaa atcaaaatca agaaaattga agacaatacg gctgccgaag
     1561 tcgagatttt aattcatttg tatcccggcg tttcaccaga caaaaccatt gatgcgctgt
     1621 ttgctttcac ggcttgcgaa acttcggtgg cgcctttggg atgtgtgatt gaagataata
     1681 agcctttgtt tgttggtgtt tcggatatgt tgaaaatttc gactgaaaga acggtcgatt
     1741 tgcttcgtca ggaattagaa attcaattag aggaattaaa aaacaaatgg catttttcga
     1801 ccttggaaaa aatcttcatt cgtgaagaaa tgtatattga tttcaaattg tattctgata
     1861 gagaatcgct ttacaaatat atgtatgacc gatttgagcc tttcaaaaaa tcatttgttc
     1921 gtgagataaa tgatgatgat ttgcaacgat tgactcagat tccgatgatt cgtattaccc
     1981 gatttgattc tgacaaagca gatgatttga ttgccaagtt ggaagacgaa atgaaggaag
     2041 ttgagcataa tttggcaaac ttgaccgatt ttgccattgc ttattttacg aagttaaaag
     2101 agaaatatgg taaaggacgc gaacgccaaa ctgaattgcg cagtttcgat aatattgaag
     2161 caacaaaagt agctttgcga aacaccaaac tctatgtcaa tagggaagaa ggtttcatcg
     2221 gaacaggatt gaaaaaagac gaatatgtta cagattgttc cgatattgac gatgtaattg
     2281 tttttctgcg agacggaaat atgatgattt gcaaagtcga tgataaaaaa tttgttggaa
     2341 aagatattat tcatgtggct gtttttgata aaggtgacaa acgcacgata tacaatatga
     2401 tttatcgtga tggaaaatcg ggaccatctt acatcaaaag atttaatgtt tcgggagtga
     2461 ctcgagataa attatacgat ttgaccaatg aaaccaaagg ttctcaaatt ctttatttta
     2521 cttgtaatcc aaatggggaa gccgaagtaa ttacgattat tttgcgccaa ataggaagta
     2581 tcaaaaaact gaagtgggat gtggattttg ccaatacagc cataaaagga cgtgcttcca
     2641 aagggaattt ggtttctaaa taccccatca agaaaattga aatcaaggaa aaaggaattt
     2701 cgaccctgaa accaagaaaa atttggtttg acgatacggt tcaaaaatta aatgtcgatg
     2761 ccagaggtga attattgggc gagtttagac caaatgataa gattcttgtg atttcacaaa
     2821 ccggaaaatt gaaagttatc attccagaat tggccactca tttcgatgaa gatatggtgg
     2881 ttttagaaaa atggcatcca aagaaaccag tttcggccat ttattatgat ggcgaaaaag
     2941 aacgttatta catcaaacgg ttcttgattg aaaacgaaaa caaagaagag acttttatca
     3001 cagaacaccc taaatcacaa cttgaaattg tttcaaccga ttaccgtcca gttgcagaat
     3061 tgatttttgc gaaagtaaaa ggcgttcaaa aagaaaatca aacggttgac atagagagtt
     3121 tcattgctgt aaaaggactg aaagcactcg ggaatcaatt gactactgag aaattaaaac
     3181 aagtcaattt gttggagcct ttgccttatg aagagcctga agaagtagtt gctgaggatt
     3241 tagaagtaca aggcaatact gaaattgagg aaggagaaat tcaactcgac gatgacggtc
     3301 aaattacttt aagtttagaa taaaaaaagc tccgaaagga gctttttagt ttgttgtcaa
     3361 tctgattttt aca
//
LOCUS       DAOSNN010000266         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_29102_length_3296_cov_5.469300, whole genome shotgun sequence.
ACCESSION   DAOSNN010000266 DAOSNN010000000
VERSION     DAOSNN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29102_length_3296_cov_5.469300"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLQ70_11025"
     CDS             <1..786
                     /locus_tag="PLQ70_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013447225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE35192.1"
                     /translation="SANMFYISAFAAFILGIYAFTLPKCPPQKLIEKGSSLSQKLGLD
                     AFKLFGTYKMAVFFIFSMFLGAALQLTNMYGDVFLSEFGNMPQYADSFVVQKSTLIMS
                     ISQFSETAFILAIPFFLKRFGIKQVMLISMLAWVLRFGFFAYGDPSFGGTSLIIMSCI
                     VYGMAFDFFNISGSLFIETTTDSKIRSSAQGLFMMMSNGFGAFFGGIISGQVIDAFFT
                     HDGVRDWHNIWLAFATYALVIAIAFSVMFKHKHNPEEVANVSH"
     gene            complement(898..1728)
                     /locus_tag="PLQ70_11030"
     CDS             complement(898..1728)
                     /locus_tag="PLQ70_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35193.1"
                     /translation="MKKSFLLLFVITSQLSIAQTKNELDFKIQYNPETKYDQTMDQTS
                     HLEMTYSGDPEIIQMLKDKGIQNPTITDNNSIIESVCKTGKLKDKTYYPLTIEFLKTT
                     SSNNKIAIPNGTIIYGKGTIGNMPTLDSIVSADLSDELKKTTLETVQSTFSQLNFPER
                     KVKVGDVFTVDSPLSIPIANAQLDMTITTTYKLVSIKNNVGNFDISQVYTMKANTGKF
                     PITATGTGKGKLEYDVIHNFNLKYQIDMDMSANLKIEKVELSIVTKSGITQTARISKI
                     "
     gene            complement(1852..2514)
                     /locus_tag="PLQ70_11035"
     CDS             complement(1852..2514)
                     /locus_tag="PLQ70_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HQE35194.1"
                     /translation="MQDLKPNEQRSQNAILLIWIALALNCISLVSSYFQYDLLQTAAN
                     GGEISTEAVSSNDNREQAIGIIQLIIFVLSAITFIQWFRRAYFNLHLRVNHLSQTEGW
                     AAGCWFVPIVNLFKPYQIMKELFQETHLFLKRNEVHTREHLSIASLGLWWTFWIIDRF
                     VGQFVFKYSMKAETIDELTTSTIAQLISNGIGIVLAIITINIITEYSKLESLLHKVKI
                     TE"
     gene            complement(2585..3133)
                     /locus_tag="PLQ70_11040"
     CDS             complement(2585..3133)
                     /locus_tag="PLQ70_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQE35195.1"
                     /translation="MSRKYKFHEKEGAYFVSFAKVNWIDVFTRDLYFSIMTESLDYCR
                     KNKGMEIYGYCIMPSHVHLIFRSALGAPSGLMRDLKGFTSRKIMKTIQDNPQESRREW
                     LLWMFERAGKKNCNVKEKQFWQQNNQPIEIWSLKVFEQKLNYIHNNPVESGFVTDPTD
                     WKYSSARNYSNNDHTILEIDLN"
BASE COUNT          905 a          537 c          593 g         1261 t
ORIGIN      
        1 tcggccaata tgttttatat atctgctttt gctgccttta ttctcggaat ttatgctttt
       61 accttgccta aatgtccacc tcaaaaattg attgaaaaag gttcttcttt gtctcaaaaa
      121 ttaggtttgg atgcttttaa attatttgga acctacaaaa tggctgtttt ctttatcttc
      181 tcgatgtttt tgggagctgc tttgcaattg accaatatgt atggagatgt attcttgtct
      241 gaatttggaa atatgcctca atacgcagat agttttgtag tgcaaaaatc tactttaata
      301 atgtctatat cgcaattttc ggaaacagcg tttatcttgg cgattccgtt tttcttgaag
      361 cgatttggaa tcaagcaagt gatgttgatt tctatgttag cttgggtgtt gcgttttggc
      421 ttctttgctt atggcgaccc ttcttttgga ggaacatcct taattattat gtcgtgcatt
      481 gtttacggaa tggcctttga cttctttaat atttcgggtt cgttatttat cgaaaccact
      541 accgattcca agattcgttc tagcgcacaa ggattgttta tgatgatgtc caatggtttt
      601 ggcgctttct ttggcggaat tatcagtgga caagtgattg atgctttttt tacccacgat
      661 ggcgtgagag actggcacaa tatctggttg gcatttgcga cttatgcttt ggtgattgcc
      721 attgcgtttt cggttatgtt taaacacaaa cacaatcctg aagaagtggc gaatgtgagt
      781 cattaacaaa aattttataa atataaaacc tcctttaaaa ctgatttaaa ggaggttttt
      841 ttgtggttga aatgctaaaa tagtagtaaa attagccaat attgatttca aacagtttta
      901 gattttcgaa attctcgcag tttgagttat tccagatttt gttacaatag ataattccac
      961 tttctcgatt tttaggttgg cagacatatc catatcaatt tggtatttga ggttgaaatt
     1021 atgaattaca tcatattcta attttccctt tccagttcct gttgcggtaa ttgggaattt
     1081 gccagtatta gccttcattg tatatacttg tgaaatatca aaattgccaa cattgttttt
     1141 gatgcttact aatttgtaag ttgtagtaat ggtcatatcc aattgtgcat tggcaattgg
     1201 aatggatagg ggagaatcta cagtaaaaac gtcaccaact ttgacttttc tttctggaaa
     1261 attcaattga gaaaaagtag attgcaccgt ttctaatgta gtttttttta attcatcact
     1321 taaatcagct gatacaatag aatctaatgt aggcatattt ccaatggttc cttttccgta
     1381 gataatagtg ccatttggaa ttgctatttt gttgttgctg cttgtagttt tcagaaactc
     1441 aattgttaat gggtaatagg ttttgtcttt tagttttccc gttttgcaaa cagattcgat
     1501 tattgagtta ttgtcagtaa tggttgggtt ttgaattcct ttgtctttca acatttggat
     1561 gatttctgga tcgcctgaat aggtcatttc taaatgactt gtttgatcca ttgtttgatc
     1621 gtatttggtt tcaggattgt attgaatttt gaaatccaat tcgtttttgg tttgagcaat
     1681 gcttagttgt gatgtaataa cgaagaggag taagaaggat tttttcattt gagtatttgg
     1741 tttgattgtg taaatatgga aaatatttca gatatgtttg agaatgatta gttcgtttct
     1801 tttagtaatt gtttgattta ttgatttgag ctttattact gttgctatat ttcattccgt
     1861 tatttttact ttgtgcagta atgattccag tttgctatat tcagtaatga tatttatcgt
     1921 aattatggct aatactattc caataccatt ggagattagt tgagcaattg tactcgtagt
     1981 caattcatca attgtttcag ctttcataga atatttaaag acaaactgtc caacaaatcg
     2041 atcaataatc caaaatgtcc accataaacc caaggaagct attgataagt gctcccttgt
     2101 atgcacctca tttcttttta aaaataaatg ggtttcctga aacaattctt tcatgatttg
     2161 atacggttta aataggttta caatgggcac aaaccaacaa cctgcagccc aaccttcggt
     2221 ctgcgataag tgattcactc tcaaatgaag attaaaataa gcccgtcgga accattgaat
     2281 aaaagtaatt gcagacaaca caaaaataat aagttgaatg ataccaatgg cttgttcccg
     2341 attgtcattg gaacttactg cttctgtcga aatttcgcct ccattggcag ctgtttgcag
     2401 taaatcatat tgaaaataac tggaaactaa tgaaatacaa ttcagtgcca aagcaatcca
     2461 aatgaggaga attgcatttt gagaccgctg ttcgtttggt tttaaatctt gcattttgtt
     2521 tttcttacta tttagtattt atttggggct agtgcaagct tcaagctcgt acccgttatc
     2581 acaatcaatt caaatctatt tctaaaatgg tgtggtcgtt attgctataa ttccttgcgc
     2641 tgctgtattt ccaatctgtg gggtcggtta caaaacccga ttcgacagga ttattatgta
     2701 tataattcag tttttgttca aacactttca aactccaaat ttctataggc tggttgtttt
     2761 gttgccaaaa ctgtttttct ttgacattgc agtttttctt tcctgctctt tcaaacatcc
     2821 aaagcaacca ttctcgtctg ctttcttgcg gattgtcttg aatcgttttc attatttttc
     2881 tggatgtaaa accttttaaa tcccgcatta atcctgatgg ggctcctaaa gccgacctga
     2941 aaattaagtg tacatgactt ggcataatac aataaccata aatttccata cccttgtttt
     3001 ttctgcaata gtctaatgat tcggtcatta tcgaaaaata tagatctctt gtaaaaacat
     3061 ctatccaatt gactttggca aaacttacaa aataagctcc ttctttttcg tggaatttat
     3121 attttcggct cattttactt ttttgtaaat ataaatattc ttacaacaaa aagctataac
     3181 ttttttgtgt gtgtagtttg tttgctcaac tttgtgggta cgagctagaa gctcgcacta
     3241 gcgctctgtt aactactgct cgcgccagct gggggttatc atttattgta ggtctt
//
LOCUS       DAOSNN010000267         3290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_29187_length_3290_cov_4.200927, whole genome shotgun sequence.
ACCESSION   DAOSNN010000267 DAOSNN010000000
VERSION     DAOSNN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29187_length_3290_cov_4.200927"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLQ70_11045"
     CDS             <1..343
                     /locus_tag="PLQ70_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQE35196.1"
                     /translation="EAIIVFLLAGIVLVAGANFLSNLISPKSDNSQKREPYESGMTTI
                     GPTWVQFKVGYYLYAILFLVFDVEVAFLIPWAVVFKEIGMVAFVEIIIFLVILGLGLA
                     YAWKKGALKWE"
     gene            334..894
                     /locus_tag="PLQ70_11050"
     CDS             334..894
                     /locus_tag="PLQ70_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013306249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HQE35197.1"
                     /translation="MGVNNTSEIPADFPGKVIPGGNGANILVTSLDDIINWGRANSLW
                     SLYFGTSCCAIEMMQTGAARHDYSRFGFEVARPSPRQADLIIIAGTIVNKMAPVLRRL
                     YDQMAEPKYVIAMGACAISGGPFYYNSYSVVKGADHVIPVDVYVPGCPPRPEALLEGM
                     LMLQAKIKTESMNNKVFPIDGFDEGL"
     gene            905..1366
                     /locus_tag="PLQ70_11055"
     CDS             905..1366
                     /locus_tag="PLQ70_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQE35198.1"
                     /translation="MTNEALQNLISLWIPELEFTEEKSQFLNITVPPEQLIPLMTQLK
                     SNSETNFDYLFCLSGVDWGKELGVVYHLESTTHRHQLVVKVKTEDRENPTFDTVCKIW
                     YTAEFHEREVFDFFGIQFTNHPNLKRLFMTEEWDGFPLRKDYVDEINMIIK"
     gene            1373..2494
                     /locus_tag="PLQ70_11060"
     CDS             1373..2494
                     /locus_tag="PLQ70_11060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194946.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQE35199.1"
                     /translation="MDTTPIINNFKSEEYQINMGPQHPATHGVLRLLLTIDGEIIKKV
                     EPDLGYIHRSIEKMCERDSYQQIVHLTDRMDYLSSHINNEAVCLTVENALELEIPERV
                     KVIRTIIAELTRIASHTLWWGVMGMDVGALTTYFYGFRDREMINDIFEETCGARLTMN
                     YNIPGGLMFDIHPNFVKRTKEFIAHFKTKLPEYDLLLSNNIIFRKRMEGIGILTKEDA
                     ISFGASGPVGRASGYSCDVRKHHSYSAYDKVKFNEVLKTEGDTYSRYQVRVLEMWESL
                     SIIEQLIDNIPEGDFFAKTNAVIKLPEGEFYQKVETARGELGVYIVSTGTKNPYRMKF
                     RSPGFSNLSLLNHIAVGGKIGDLVATMATLDLVIPDIDR"
     gene            2505..>3290
                     /locus_tag="PLQ70_11065"
     CDS             2505..>3290
                     /locus_tag="PLQ70_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQE35200.1"
                     /translation="MNITKNIHEWLFSLMPETTASIVEMVLIAVVYLAIFAVAGLYLV
                     LLERRVAAWFQLRLGPNRVGYQGLLQTMADALKLVSKELTGTLKADKFLYNLAPYFVI
                     VTALMAMSLFPFTKELHAFDINIGIFFLIAISSIGVIGILLGGWSSNNKFALIGAMRS
                     GVQTISYELSVGLSLLTMVLMTGSLQLSQIVEVQEKGGWLIVQGHIPALIAFCIYMIA
                     GTAETNRAPFDLVEAESELGAGFHTEYSGMKFAYFFLAEFINMF"
BASE COUNT         1031 a          621 c          657 g          981 t
ORIGIN      
        1 agaagcaatt atagttttct tattagccgg aatagtcttg gtcgccggtg ccaatttcct
       61 ctctaattta atttcaccaa aatcagacaa tagccaaaaa cgggaaccgt atgaaagcgg
      121 aatgaccacc attggaccaa cttgggttca gtttaaagtt ggctactatt tgtatgctat
      181 tttattcttg gtttttgatg tcgaggtagc ctttttgata ccttgggcag tagttttcaa
      241 agaaattgga atggtcgctt ttgtcgaaat catcatcttt ttagtaatat taggtttagg
      301 cttggcctac gcttggaaaa aaggagcatt aaaatgggag taaataacac atctgaaata
      361 ccagcagatt tcccgggaaa agtaattcct ggaggtaatg gagccaatat tttggtcact
      421 tcattagacg acatcatcaa ttggggacga gcaaactcgc tttggtccct ttattttgga
      481 acaagctgtt gcgctatcga aatgatgcaa accggagcag ctcgtcacga ttattctaga
      541 tttggattcg aagtagcaag accttcgcca agacaagccg atttaattat cattgctgga
      601 actattgtca acaaaatggc acccgttcta cgcagattgt acgatcaaat ggcagaacca
      661 aaatatgtaa tcgccatggg agcttgcgcc atttctggtg gtcctttcta ttataattcg
      721 tattctgtcg taaaaggagc cgatcacgta atccctgtag atgtttacgt tccgggttgt
      781 ccacctcgtc cagaagcttt gctcgaagga atgctaatgc ttcaagctaa aatcaaaaca
      841 gaaagtatga ataataaagt tttccctatc gacgggtttg acgaaggact ataataccaa
      901 aattatgaca aacgaagcct tacaaaattt aataagcctt tggattccag aattggaatt
      961 cacagaagaa aaatcacagt ttctaaatat caccgttcca cccgaacaac ttatcccact
     1021 aatgacgcaa ttaaaaagta attccgaaac gaatttcgat tatttgtttt gtcttagcgg
     1081 tgtggattgg ggcaaagaat taggtgttgt ttaccattta gaatctacaa ctcaccgcca
     1141 tcaattggtc gtaaaagtaa aaaccgaaga ccgagaaaac ccaacatttg atacggtttg
     1201 caaaatttgg tacacggcag aatttcacga aagagaagtt tttgatttct tcggaattca
     1261 attcaccaat catcccaatc taaaaagact ttttatgacc gaagaatggg acggatttcc
     1321 gttgagaaaa gattatgtag atgaaattaa tatgattatc aaataaggaa ctatggacac
     1381 aacacccata ataaacaact ttaaatccga agaataccaa atcaatatgg ggccgcaaca
     1441 cccagcaact cacggtgttt tgcgactttt attgactatt gatggtgaaa taatcaaaaa
     1501 agtagaaccc gatttaggat acatccaccg ttctatcgaa aaaatgtgtg aacgcgacag
     1561 ctatcagcaa atcgtgcatt tgaccgaccg aatggattat ctttcttcac atatcaacaa
     1621 cgaagccgtt tgcctaacgg ttgaaaatgc cctagaatta gaaattcctg aacgtgtaaa
     1681 agtcattcga accatcattg ccgaattgac ccgaatcgca tctcacactt tgtggtgggg
     1741 cgttatggga atggatgttg gcgctttgac cacctacttc tacggtttta gagacagaga
     1801 aatgatcaac gacattttcg aagaaacctg tggcgcacgt ttgacaatga actacaacat
     1861 tccaggagga ttgatgtttg acattcaccc aaactttgta aaaagaacca aagaattcat
     1921 tgcgcatttc aaaaccaaat taccagaata cgatttgctt ttatccaata atatcatctt
     1981 cagaaaaagg atggaaggca ttggtatttt aaccaaagag gatgcgattt cgtttggagc
     2041 ttctggccct gtgggtcgtg cttctggcta ttcttgcgat gtaagaaaac accattcgta
     2101 tagtgcttac gacaaagtaa aattcaacga agttttaaaa accgaaggcg atacttattc
     2161 ccgttaccaa gttagagttt tagaaatgtg ggaatccttg tcgatcatcg aacaattaat
     2221 tgacaacatt cccgaaggtg atttttttgc caaaaccaat gcagtaataa agttgccaga
     2281 aggcgaattt taccaaaaag tagaaactgc aagaggagaa ttaggggttt acatcgttag
     2341 tactggaaca aaaaatccgt acagaatgaa attccgttcg ccaggattct ctaatttatc
     2401 cttattaaat catattgctg taggtggaaa aattggagat ttagtagcaa ctatggcaac
     2461 attagacctt gtaatccctg atatcgaccg ataaaattaa ttttatgaac attacaaaaa
     2521 atattcacga atggcttttt agcctgatgc cagaaactac tgcaagcatt gtagaaatgg
     2581 tattgatagc cgtagtttac ctagctattt ttgcagttgc tggtttatac ttggttttac
     2641 tcgaaagaag agtcgccgct tggtttcaat tgcgtttggg tccaaacaga gtaggttacc
     2701 aaggtttatt acaaactatg gccgatgcct tgaaattggt ttccaaagag ttaacaggaa
     2761 ccctcaaagc cgacaaattt ttatacaatt tagctcctta ttttgttatc gtaactgcct
     2821 tgatggcgat gtcgcttttc ccttttacga aagagttaca cgcctttgac atcaatattg
     2881 gtattttctt tttaatagct atttcctcga ttggtgtaat cggaatttta ttaggaggat
     2941 ggagtagcaa taataaattt gccttgattg gtgcaatgcg aagcggtgtt caaaccatta
     3001 gctacgaatt gtccgttgga ttatcactgc tcacaatggt tttaatgacc ggttctttgc
     3061 aactttcgca aatcgtagaa gttcaagaaa aaggaggttg gcttatcgtt caaggacata
     3121 ttcctgccct aatcgctttt tgcatttata tgattgctgg aactgctgaa acgaacaggg
     3181 cacctttcga cttagtagaa gccgaatccg aattgggtgc tggttttcac accgaatact
     3241 cgggaatgaa gtttgcctat ttcttcttgg ccgaatttat caatatgttt
//
LOCUS       DAOSNN010000268         3273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_29369_length_3273_cov_4.567744, whole genome shotgun sequence.
ACCESSION   DAOSNN010000268 DAOSNN010000000
VERSION     DAOSNN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29369_length_3273_cov_4.567744"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..1291
                     /locus_tag="PLQ70_11070"
     CDS             221..1291
                     /locus_tag="PLQ70_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQE35201.1"
                     /translation="MKTIALLSLLLISISSCSKDDNTIPPTPSETMYFPSNTNSTWET
                     KSISSLNWNQNAIQPLKDYLAQKNTKSFMILVNGRIVMEEYFNGHSASADWEWNSAGK
                     TLVGTTIGVAQQNNLVNINNKASDYLGLEWTSMPLAKENLITVRNLLTMTSGNDDTKQ
                     YVIKSNLTYVADAGTRWAYSNIFQKLTDVVASASNKTFETYFNEKIKNKIGMDGFWNF
                     GTIYTIYHSTTKSMARFGLLALNKGKWNNEQIINESFFNESIATSQNINLSYGYFWWL
                     NGKTNYMIPGEQTVYQGYLVPNAPSDMYAAMGAKDQRIYVIPSKKMVIIRMGDASDPA
                     NPNFAVSGFDNELWGKINALIN"
     gene            complement(1336..2778)
                     /locus_tag="PLQ70_11075"
     CDS             complement(1336..2778)
                     /locus_tag="PLQ70_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HQE35202.1"
                     /translation="MMKKSVITLIVFCLSLTAVAQKVYDVKKYGAKGDGKTNDAIAIQ
                     KAIDACSKTGGRVLIPAPFTFLTGPFDVKSGIDLHIEAGAKLLASPDEKLYTKSAFRS
                     NPGEGTIWIGGKNIENFSISGTGQIDGNGISFMGEELEDSYVLKPFVGLDPRPHVLTI
                     IGGKNIRIRDIHIGNSAYWTVHLIGCNDVVISGITLLNSLKVRNSDGIDLDHSKNVRI
                     SDCYIESGDDCICLKNRREFEEFGACENITVTNCTMTSSSCAIKIGSENMDAIRQVVF
                     NNCIIKNSNRGLGIQNRDEGTVSDVIFSNIIVEGRLTTDTWWGKAEPIYITAYRRAKG
                     NHKDANWRFPKGATEGKVGKISNILFSNIQCTGENGVYVSAESNDKISNIVFDNVGVF
                     IDKTTSNPGGIYDRRPSNIDGFVKGSTSGFYFDAAESIKLQNCSVQWGKNKPDYFKYA
                     VESKNVDSLIINNLEGQSAFPDKLEAVKKE"
     gene            complement(2838..>3273)
                     /locus_tag="PLQ70_11080"
     CDS             complement(2838..>3273)
                     /locus_tag="PLQ70_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE35203.1"
                     /translation="TILPTQVFGEVIKNGGTGSIVNISSVAADHALTRVLGYSLAKSA
                     IDSYTKWFAVELGNRFGDAIRMNSIAPGFFLAEQNRALLTNEDGSLTERGNLILQNTP
                     FSRFGNPDELTGALVWLLSDASKFVSGTKITVDGGFSVNSGV"
BASE COUNT         1068 a          647 c          533 g         1025 t
ORIGIN      
        1 tttgttgggg aatatttttg ctaatttttg tatcggaaag taaaccagtc aactatttta
       61 cacccttttt aggcggactt tttattgcta ctgtgggttg actcacaaag taggataaaa
      121 aaataaaaag cgaaaactaa aaaaacgaaa cttaggataa tttaatcata tcattaaacc
      181 atttagctaa taaaatgtca aatacggaaa taatgaaact atgaagacaa tagccctact
      241 ttctttatta ctgatatcaa tttctagttg tagtaaagat gataatacga ttccacctac
      301 tccatcagaa acgatgtatt tcccttctaa tactaattcg acttgggaaa ccaaatcaat
      361 ttcaagtttg aattggaatc aaaatgccat ccaacctttg aaagattatc ttgctcaaaa
      421 aaacacaaaa tcatttatga tacttgtgaa tggtagaata gttatggaag aatattttaa
      481 tggtcacagc gcttctgcag attgggaatg gaatagtgct ggtaaaactt tagtcggcac
      541 aacaatagga gtcgctcaac aaaataatct agtgaatatt aacaataaag catccgatta
      601 tttaggttta gaatggacaa gtatgccttt ggcaaaagaa aatttgataa cagtgcgaaa
      661 tctattgaca atgacatcag gcaatgatga tacgaaacaa tatgttatta aatcaaattt
      721 gacctatgtt gctgatgctg gaaccagatg ggcttatagt aacatttttc aaaaactaac
      781 cgatgtagtt gcaagtgcta gtaacaaaac atttgaaacc tatttcaacg aaaaaataaa
      841 gaacaaaatt ggaatggatg gtttttggaa tttcggaaca atttatacca tatatcacag
      901 caccactaaa agtatggcaa gatttggact tttggcattg aacaaaggaa aatggaacaa
      961 tgaacaaata attaatgaat ccttttttaa tgaaagtata gctacttctc aaaacatcaa
     1021 tctttcttat ggttactttt ggtggctcaa tggaaaaacc aattatatga ttccgggcga
     1081 acaaactgtt tatcaaggct atttagttcc taatgctcct tccgatatgt atgcagctat
     1141 gggcgcaaaa gaccaacgca tttatgtaat tccaagtaaa aaaatggtaa tcatcagaat
     1201 gggtgatgct tctgacccag caaatccaaa ttttgcagtg tcaggttttg ataacgaact
     1261 atggggtaaa attaatgctt tgataaatta aaacaaaaac gtccaaacgt ctgtgtaatt
     1321 acaaactcaa tccccctact ctttttttac cgcttctaat ttatcaggaa atgcagattg
     1381 tccttcgaga ttattgatga ttaaggaatc cacattttta ctttcgacag catatttaaa
     1441 ataatcgggt ttgttctttc cccattgtac agagcagttt tgcaatttta tactttcggc
     1501 agcatcaaaa taaaagccag aagtgctccc ttttacaaaa ccatcaatat tagaggggcg
     1561 tctatcgtaa attcctcctg gattactggt tgttttatca ataaaaacgc caacattatc
     1621 aaaaacaata ttgctgattt tgtcattgga ttcggcactt acataaacgc cattttcacc
     1681 cgtacattga atattagaga atagaatatt gctgatttta cccactttgc cttcagtagc
     1741 tcctttgggg aaacgccaat tggcatcttt gtgattgcct tttgcacgac gataagcggt
     1801 aatataaatg ggttccgctt ttccccacca agtatcggta gtcaatcgac cttccacaat
     1861 tatattggaa aaaataacgt cgctcacggt tccttcgtcc cgattttgaa ttccaagtcc
     1921 acgattgcta tttttaataa tgcaattatt gaacacaact tgcctaatag cgtccatatt
     1981 ttcggaacca atcttgatgg cacaactgct gctagtcatc gtgcaattgg taaccgttat
     2041 gttttcgcaa gcaccaaatt cttcaaattc tcttcggttt tttaaacaaa tgcaatcgtc
     2101 accgctttcg atataacaat cactaattcg aacattttta gaatgatcta aatcgattcc
     2161 atcgctattg cggactttca aactattgag caaagtaatt ccgctaataa ccacatcgtt
     2221 acaacctatt aagtgaaccg tccaataagc tgaatttcct atatgaatgt ctctaattcg
     2281 aatgtttttt ccgccaatga tagtcaatac atgcggtctt ggatccaaac ccacaaaagg
     2341 tttcaataca taagaatctt ccaattcttc gcccataaaa gaaataccgt ttccatcgat
     2401 ttgacctgta ccactgatgc taaaattttc tatgtttttg cccccaatcc aaatcgttcc
     2461 ttcaccggga ttagaacgaa aagcactttt ggtatataat ttttcgtcag gactcgccaa
     2521 tagttttgct cccgcttcaa tatgcaaatc aatacccgat ttcacgtcaa aaggacctgt
     2581 caaaaaggtg aacggcgcag gaatcaatac ccgacccccc gttttactac aagcgtctat
     2641 tgctttttga atggcaatgg catcattagt tttgccatcc cctttggctc cgtatttttt
     2701 gacatcatac actttttgcg ccacggcagt caatgaaaga caaaaaacga ttaaagtaat
     2761 tacagatttc ttcatcattg tttttattta atgaattctc gaaactgaac ttcaagaaat
     2821 tcttttatta acatttttta taccccgcta ttcacagaga agcctccatc tacggtgatt
     2881 tttgttccag aaacaaattt ggaagcatca cttaatagcc aaaccaaagc tcctgttagt
     2941 tcgtctggat ttccaaaacg gctgaaagga gtattttgaa gaatcaaatt ccctctttcg
     3001 gtcaaacttc catcttcgtt ggtcaataag gctctatttt gctcggccaa gaaaaaaccg
     3061 ggcgcaatag aattcattct tatggcatcg ccaaatctat ttcccaattc gaccgcaaac
     3121 catttggtat aagaatcgat ggctgatttt gccaaactat atcccaatac ccttgttaat
     3181 gcgtgatcgg cggcaacgga cgaaatattg acaatgctac ctgttccacc attttttatg
     3241 acttcgccaa aaacttgggt cggtaaaatg gtt
//
LOCUS       DAOSNN010000269         3262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_29486_length_3262_cov_7.256314, whole genome shotgun sequence.
ACCESSION   DAOSNN010000269 DAOSNN010000000
VERSION     DAOSNN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29486_length_3262_cov_7.256314"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLQ70_11085"
     CDS             <1..222
                     /locus_tag="PLQ70_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQE35204.1"
                     /translation="TNSSFQVNGKSTFKEEFVTAGGIDLKEINFKTMESKLHENLYFA
                     GEIVNIDAVTGGFNFQNAWTSGFILANGL"
     gene            complement(287..718)
                     /locus_tag="PLQ70_11090"
     CDS             complement(287..718)
                     /locus_tag="PLQ70_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HQE35205.1"
                     /translation="MSIAELFDSEFKHRVKGRFSAVVRVLYADGEFDPDEKNFLDKLA
                     IKLDISEEEYKEILKNPLKYPLNPPYLYVERLESLYNISRVVHHDHHLGDKQETLLRK
                     FAIALGFTTANVNYIVNKALALIDKKVDLDTFISEMQNMNR"
     gene            918..1511
                     /locus_tag="PLQ70_11095"
     CDS             918..1511
                     /locus_tag="PLQ70_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQE35206.1"
                     /translation="MSEFWIFFEKGLRHILNLTAYDHILFLMALTIPYAFKDWKKLLL
                     LVTIFTVGHTLALLLSVYEIVIIKVNLIEFLIPITILIIAFFNLFTAGKSSKGESISV
                     IGFVTLFFGIVHGLSFAGYFKTLVSGSSQSKLLPLTEFSLGIEASQIVVVFIVLILSY
                     IVQTVFRFSKRDWTLVMSSFIIGVVLPMIISSKIWIR"
     gene            1520..1948
                     /locus_tag="PLQ70_11100"
     CDS             1520..1948
                     /locus_tag="PLQ70_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HQE35207.1"
                     /translation="MEKKKLNKYDKAYLRIAKEWSLLSYCKRKQVGAIIVRDKMIISD
                     GYNGTPSGFENCCEDEAGLTNWYVLHAEANAILKVAKSTQTCEGATLYITLSPCKECS
                     KLIHQSGIKRVVYQLGYRDTSGVDFLEKAGVIVEQIEVLD"
     gene            1950..>3262
                     /locus_tag="PLQ70_11105"
     CDS             1950..>3262
                     /locus_tag="PLQ70_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQE35208.1"
                     /translation="MKTNPKYLPILLFATLAFGILLGGWLNFPGQNQFLAKNNSKNKL
                     NKLLDFIDNEYVDKVNADTITNRAIDNILAQLDPHSVYIPPSEQAQVAESMRGEFVGI
                     GVNFYMYKDSVAIIKPVENGPSEKAGIQSGDRILYADKTKLFGRKLPTDSLFSKLKGK
                     EGSEILVTVYRKSENKKINIKIKRGVIPIKSVDAALLLNPTTGYIKINRFAESTFDEF
                     KTGLTQLKEKGIKSLVIDLRDNGGGYLEQAVAIADEFLTDKQLIVYTKDKNGTIEKTI
                     ATKTGSFENGKLFVLINENSASASEILAGAIQDNDRGTIVGRRSFGKGLVQREMDFED
                     GSAVRLTIARYYTPTGRSIQKPYSKGTEDYMKESESRFKSGELYHKDSIKTPNTPKFK
                     TPKGKIVYGGGGIVPDIFVPLELESSKEHTAYLLQTGILGQFVFE"
BASE COUNT         1129 a          552 c          573 g         1008 t
ORIGIN      
        1 acgaattcta gttttcaagt gaacggaaaa agcactttca aggaagaatt tgtaaccgct
       61 ggaggcattg atttaaaaga aattaacttt aaaactatgg aaagtaaact gcacgaaaat
      121 ttatatttcg ctggagaaat tgtaaatata gacgcagtaa ctggaggatt taattttcag
      181 aatgcttgga caagcgggtt tattttggca aatggattgt agttattaat aaaattaaat
      241 tgggtgcttc ttgccttaat ctagcaacaa acacccaatt ttattactat cgattcatat
      301 tttgcatttc actaataaaa gtatctaaat ctactttttt atcaatcaaa gctaatgctt
      361 tatttactat ataattgacg ttagcagtag taaaacctag agctatagca aattttctca
      421 aaagcgtttc ttgcttatcg cctaaatgat gatcgtgatg caccactctc gaaatattgt
      481 ataaactctc caacctttcg acatataaat aaggtggatt taatggatat ttcaaaggat
      541 tcttcaaaat ctctttatat tcttcctcag aaatatcaag ttttatcgct aatttgtcta
      601 aaaaattttt ctcatcggga tcaaattccc catctgcata caaaacacgt actaccgcag
      661 aaaaacgtcc ttttactcgg tgtttaaatt cactatcaaa taattcagct attgacataa
      721 taaacagatt ttaaagttgt cctataaaga tacaacttat ttcgattttt tcaaatacca
      781 aaatcaatat tttcatatga atttctctgc ttagataaaa taagtgaagc aagaaatgct
      841 tttaacagta tcctataaaa ctaatttcaa aataaattgt aagtttgaca acccctaatc
      901 ttataaaact attagtaatg tcagaatttt ggattttttt cgaaaaaggc ttgcggcata
      961 ttctaaacct aaccgcttat gaccacatat tatttctaat ggctttgacg attccgtatg
     1021 ctttcaaaga ttggaaaaaa ttattgcttt tggttactat tttcaccgtt ggacacacct
     1081 tagcattgct tttgtctgtt tatgaaattg tgattataaa agtcaatttg atcgaatttt
     1141 taattccaat aaccattcta atcatcgctt tttttaatct tttcacggca ggaaaatctt
     1201 caaaaggcga aagcattagc gtaattggat tcgtaacttt gttttttgga attgtacacg
     1261 gtttgagttt tgcgggatat tttaaaacct tagtaagcgg ttcgtcacag tcaaaattat
     1321 tgcccttaac tgagttttca ttagggatag aagcatcgca aatagttgtg gtttttattg
     1381 tattgatact ttcgtatatt gttcaaacgg tctttagatt ttcaaaacgc gattggacac
     1441 tcgtaatgtc gtcatttatc atcggagttg ttttgccaat gatcatttcg agtaaaattt
     1501 ggattagata aaattaaaaa tggaaaagaa aaaattaaat aaatacgaca aagcctatct
     1561 ccgaattgcc aaagaatgga gccttttgtc ctattgcaaa cgcaaacaag tgggtgctat
     1621 aatcgttaga gataaaatga ttatttctga cggatacaat ggaacgcctt ctggttttga
     1681 aaattgctgc gaagacgaag caggtttaac caattggtat gtacttcacg cagaggcaaa
     1741 tgcgattcta aaagtagcca aatctaccca aacttgcgaa ggagcaacac tttatatcac
     1801 gctttctcct tgtaaagaat gtagcaaatt aattcaccaa tcaggcataa aaagagtggt
     1861 ttatcaatta ggttaccgag acacttctgg agttgatttt ctagaaaaag ctggtgtaat
     1921 agtagaacaa attgaagttt tagactaaaa tgaaaacaaa ccctaaatac cttccaatac
     1981 tcctttttgc aacacttgcc tttggaattc ttttgggtgg atggctgaat tttcctgggc
     2041 aaaaccagtt tttggctaaa aacaattcaa aaaacaaact caataaatta cttgatttta
     2101 ttgacaacga atatgtcgat aaagtaaacg ctgacaccat taccaatcga gcaatcgaca
     2161 acattttggc acaattggat ccgcattcag tctatattcc acccagcgag caagcacaag
     2221 ttgccgaaag tatgcgtggc gaatttgtgg gaattggggt gaatttctat atgtataaag
     2281 attctgttgc gataataaaa ccagtcgaaa atgggccttc ggaaaaagcg gggattcaat
     2341 ctggcgaccg aattctttat gccgacaaga ccaaattgtt tggacgaaaa ttacccacag
     2401 atagtttgtt ttctaaacta aaaggaaaag aaggttccga aattttagtc accgtttatc
     2461 gaaaatccga aaacaaaaaa ataaacatta agatcaaacg aggtgtcatt cctataaaaa
     2521 gtgtggatgc agccctactt ttgaacccaa ctacaggtta cataaaaatt aatcgatttg
     2581 ccgaaagcac tttcgacgaa ttcaaaacgg gtttgacaca actgaaagaa aaaggcataa
     2641 aatcactcgt tatcgatctt cgtgacaatg gcggtggcta tctcgagcaa gctgtggcga
     2701 ttgcagacga atttctaaca gacaaacaat taattgttta taccaaagac aagaatggca
     2761 caatcgaaaa aacaattgct acaaaaacag gtagtttcga aaacggaaaa ttatttgttt
     2821 taatcaacga aaacagtgca tcggcgagcg aaatattggc tggagccatt caagataatg
     2881 atagaggaac tattgtaggt cgtcgttctt ttggaaaagg attggtgcaa agagaaatgg
     2941 attttgaaga tggctcagcc gttcgattga caattgctcg atattacact ccaacaggac
     3001 gttcgattca aaaaccttat tcaaaaggta ccgaagatta tatgaaagaa tctgaatcgc
     3061 gttttaaaag cggagaattg tatcataaag acagcataaa aacaccaaac acacccaaat
     3121 ttaaaacccc aaaaggcaaa atagtatatg gtggtggcgg gattgttccc gatatttttg
     3181 tccctcttga attggaatcg agcaaagaac acacggctta tttattgcaa acaggaattt
     3241 tgggccaatt tgtttttgag ca
//
LOCUS       DAOSNN010000270         3245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_29696_length_3245_cov_5.688401, whole genome shotgun sequence.
ACCESSION   DAOSNN010000270 DAOSNN010000000
VERSION     DAOSNN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29696_length_3245_cov_5.688401"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2115)
                     /locus_tag="PLQ70_11110"
     CDS             complement(<1..2115)
                     /locus_tag="PLQ70_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HQE35209.1"
                     /translation="MTNIQFIAKSVQTAAINIQNTVQLLEEDCTIPFISRYRKDKTGN
                     LDEVQIEQIAKFQKDYEVIVKRKEAILKSIEEQNALTPELDKKIQQSFDLQELEDFYL
                     PFKKKKKTKADVARENGLEPLAKIIMAQNNDDVDFLATKYLNDNVINEDAALQGARDI
                     IAEWINENIYVRKQLRRLFQRKAIITTKVIKTKKEEQEAQKFNQYFDWSEPLTKAPSH
                     RLLAMLRAENEGFIKFKVEVDIDEAYDIIDELVLKGQNKTTPHVQLAIEDSYKRLLNP
                     AISNEILQEAKAKADANSIQVFANNLGQLLLAPPLGEKRILAIDPGFRSGCKVVCLDE
                     KGDLLYNETIYPHAPQNETAMAMKKIKSMVNAYNIDAISIGNGTASRETEFFIKKIAF
                     DKPVQVFIVSEAGASVYSASKIAREEFPNYDVTVRGSVSIGRRLSDPLAELVKIDPKA
                     IGVGQYQHDVDQTKLKEELDSTVIRCVNSVGININTASKHLLSYVSGIGEKLAENIVQ
                     YRSENGPFEDRKQLKKVPRLGEKAYQQGVAFIRISNAKNPLDNSAVHPEAYPIVEKMA
                     KDLKLSVNDLIANKEKTALIKVENYITPEIGLLTLKDIIKELEKPGLDPRKSAKVFEF
                     DPNLKSIKDLKTGMILPGIVNNITNFGCFVDLGIKESGLVHISQLKAGFVSDVNEVVK
                     LHQHVEVKVTEVDEDRKRIQLTM"
     gene            complement(2194..2886)
                     /locus_tag="PLQ70_11115"
     CDS             complement(2194..2886)
                     /locus_tag="PLQ70_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35210.1"
                     /translation="MERINLKNDLKVDLKLEKIWSDRYKEKDEIIAPLYQIEVLEKEV
                     TFLSINPSLPPTGRIDATKGYYPHPPYPIIDYNKDKKEIYPFFRKFYEIGEALNQPWT
                     MLDLLYERESTQAELEIKYNPKNITEDDKNFLQNQIKLTFEILESIRPKVVVISNAGT
                     DKLIHFNIKDLKIVQELPCEGNGFIYKLNGIPFISNESRFLGSPQHFNRSKKDGRLEK
                     LVNEIKRVSNTK"
     gene            complement(2997..>3245)
                     /locus_tag="PLQ70_11120"
     CDS             complement(2997..>3245)
                     /locus_tag="PLQ70_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009641034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HQE35211.1"
                     /translation="SDCLNIQIICSFNTDLSKVDSALMRKGRLIAKYEFKALEVGKAQ
                     QLSDKLGFQTEILTPTILTDIYNQNETDFQEKKRVIGF"
BASE COUNT          893 a          620 c          513 g         1219 t
ORIGIN      
        1 catcgtcaac tgaattctct ttctatcctc atcaacctct gttactttta cctcaacgtg
       61 ttggtgtaac tttaccactt cattcacatc gctcacgaag ccagctttga gttgagagat
      121 gtgaaccaaa ccactttcct tgattcccaa atcgacaaaa caaccaaaat tggtgatgtt
      181 gttgacaatt cctggtaaaa tcattccggt tttcaagtct tttattgatt ttaaattggg
      241 gtcaaattca aaaactttgg ctgattttct agggtctaat cctggttttt cgagctcttt
      301 gatgatgtct tttaaagtca acaatcctat ttctggtgta atataatttt cgactttaat
      361 caaagctgtt ttctctttat tggcaatcaa atcatttacc gaaagtttta aatctttggc
      421 cattttctcc acaatcggat aagcctcagg atgcacggcc gaattatcca acgggttttt
      481 agcattcgaa attcgaataa aagcaacacc ttgttgatac gctttttcgc ccaaacgagg
      541 cacttttttc aattgttttc ggtcttcgaa agggccgttt tccgaacggt attgtacgat
      601 gttttcggcc agcttctctc ctattccgct cacgtaactc aacaaatgtt tgctcgctgt
      661 gttgatgttt attccaaccg aattcacgca acggattact gtcgaatcta attcttcttt
      721 caattttgtt tgatctacat catgttggta ttgccctact ccaatggctt ttgggtcgat
      781 ttttaccaat tcggccaacg gatccgacaa tcgtcttccg atagaaaccg aaccacgaac
      841 cgttacatca taatttggaa attcctctct cgcaattttc gaagctgaat acaccgaagc
      901 gcctgcctcc gaaacaataa agacctgaac cggtttgtca aaagcaattt tcttgataaa
      961 aaactccgtt tctctggaag cagttccgtt tccgatggaa atggcatcaa tattataggc
     1021 atttaccatc gattttatct ttttcatcgc cattgcagtt tcattttgcg gcgcgtgcgg
     1081 ataaatggtt tcgttgtaca gcaaatcgcc tttttcgtcc agacaaacca ctttacaacc
     1141 tgaacgaaaa ccgggatcaa tagccaaaat tcgtttttcg cccaaaggtg gtgccagcaa
     1201 caattgcccc aaattattcg caaaaacctg aatcgaattg gcatccgcct tggctttggc
     1261 ttcctgtaaa atttcgtttg aaatggcagg attcaacaat cttttatagg aatcttcgat
     1321 ggctaattga acgtgtggcg tagttttatt ttgccctttc aaaaccaatt catcaatgat
     1381 atcataagct tcgtcaatat caacttctac tttaaacttg atgaaacctt cattttcggc
     1441 acgaagcata gccaacaatc tgtgcgaagg cgctttggtc aatggttctg accaatcgaa
     1501 atattgattg aatttttgag cttcttgttc ctcttttttg gtttttatca ccttggttgt
     1561 aatgattgct tttctttgaa aaagtcttcg caactgttta cgaacataaa tattttcgtt
     1621 aatccattcg gcgataatat ctcttgcgcc ttgcaaagcc gcatcttcat taatcacgtt
     1681 atcgttcaaa tatttcgtcg ccaagaaatc gacatcgtcg ttgttttgag ccattatgat
     1741 ttttgccaag ggttccaatc cgttttcgcg agcgacatca gccttggttt ttttcttctt
     1801 tttgaacggc aaataaaaat cctccaattc ctgtaaatcg aagctctgtt ggattttttt
     1861 atccaattcg ggtgttaacg cgttttgctc ttcaatcgat tttaaaatgg cttcttttcg
     1921 ttttacaatg acttcgtaat ccttttggaa tttggcaatt tgttcgattt gaacttcatc
     1981 caaatttcct gttttatctt ttcgataacg ggaaataaaa ggaatcgtgc aatcttcttc
     2041 taataattga acggtgtttt gaatatttat ggctgctgtt tgaacagact tggcgatgaa
     2101 ttgtatgttg gtcatttttt gatttttcta aacgcaaaga agcgaagttt tcgcaaagtt
     2161 cgcaaaggtt ttaggtttaa aaataaaaaa ttattattta gtgtttgaaa ctcttttaat
     2221 ttcatttact aacttttcta atcttccatc tttttttgac ctgttgaaat gttgaggact
     2281 tcctaaaaac ctactttcat ttgaaataaa aggaattcca tttaatttat aaataaaacc
     2341 atttccttca caaggtaatt cttgtacaat ttttaaatct tttatattaa aatgaataag
     2401 tttgtctgtt cctgcatttg aaataacaac aacttttggt cttatgcttt ctaaaatttc
     2461 aaatgttagt tttatttgat tctgcaagaa attcttatcg tcttcagtta tgtttttcgg
     2521 attatattta atctccaatt ctgcttgtgt agattctctt tcgtacaata aatctaacat
     2581 agtccaaggt tgatttaatg cttctcctat ttcataaaat ttacgaaaaa aaggataaat
     2641 ttctttttta tctttattat aatcaattat tggataaggt ggatgtggat aataaccctt
     2701 tgtggcatca attcttccag taggtggtaa tgaaggattt attgaaagga aagttacttc
     2761 tttttctaac acctctattt ggtataaagg ggcaattatt tcgtcttttt ctttgtatct
     2821 atccgaccaa attttttcta attttaaatc aacttttaaa tcatttttta gattaattct
     2881 ttccattttt tactttttaa tagattgcta ataatatttt cacgaatgta aacttatcta
     2941 taacgaaaaa tttacggttt tccgtaattg agtttttatt gttttttatt gaaaaactaa
     3001 aacccaatca ctcttttctt ttcttgaaaa tcggtttcat tttggttgta aatatcagtt
     3061 agaatagttg gtgtgagaat ctctgtttga aagcctaatt tatctgaaag ctgttgcgct
     3121 ttgccgactt ctaatgcttt gaactcgtat tttgctatca aacgtccttt ccgcattaat
     3181 gccgaatcta cttttgataa atcagtgttg aacgaacaaa taatctgaat attcaagcaa
     3241 tccga
//
LOCUS       DAOSNN010000271         3236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_29799_length_3236_cov_5.399246, whole genome shotgun sequence.
ACCESSION   DAOSNN010000271 DAOSNN010000000
VERSION     DAOSNN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29799_length_3236_cov_5.399246"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..677
                     /locus_tag="PLQ70_11125"
     CDS             12..677
                     /locus_tag="PLQ70_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQE35212.1"
                     /translation="MSSLFLLATLFASCEATKNANNTQKGAGIGAAAGALIGGIIGNN
                     SKIGTAGGALIGAAIGGGSGALIGNKMDKQAREIDQALPGADVERVGEGIRLVLKEDA
                     VRFDTGKATLTTQAKANLDKLVPVFKDYADTNIEIYGYTDSTGSPEFNLTLSQKRAES
                     VKDYLVSKGLLVSRFKTTGLGIADPIATNDTAEGRTQNRRVEFAIMANDKMVQDAKKE
                     AGQ"
     gene            782..1015
                     /locus_tag="PLQ70_11130"
     CDS             782..1015
                     /locus_tag="PLQ70_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35213.1"
                     /translation="MVNSKKHSIDKKQDSVQEPTTDYKATFKEKLDPSNTDEQEILLQ
                     KLLAKGLEESEMGIGISNEEMKRITKLKYPFLK"
     gene            1012..1317
                     /locus_tag="PLQ70_11135"
     CDS             1012..1317
                     /locus_tag="PLQ70_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35214.1"
                     /translation="MIYRIEWKSIATRTYFGEINFILSKWNEAEVQKFEDLVYDFLEI
                     VSKNPKTGIYKPKNNCYSLVISKQTTLYYKIIEDKSQIDLILFWNNKQNPDDLIKLL"
     gene            1332..2465
                     /locus_tag="PLQ70_11140"
     CDS             1332..2465
                     /locus_tag="PLQ70_11140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQE35215.1"
                     /translation="MQLSVIILNYNVRYFLELCVSSVQNAIQNIDAEIIVVDNNSQDD
                     SCAMMKQRFPNVKLIENKENSGFPKGNNIGVAIANGEYICILNPDTVVAEDTFTKVLA
                     FAKKQEDLGIVGVKLIDGTGNFLPESKRGIPTPFVAFTKITGLYKIFSKSFGKYYAQH
                     LSENETGKVEILVGAFMLMKRDLYNEIGGFDEKCFMYSDDIDLSYMVLQKGKSNYYFH
                     ETSVIHYKGESTFRDGTYMKRSREAMNFFYKKHFKVSFLFSVFMEIGIVFFSFAKMFW
                     GNPKSKLKPNNYILVSNNLILKEKLENSLAKPIQLQDISHSIQENFTGEIILDQNQFN
                     FKTIINFFEVHKNKGFTFKILPQNSHFVIGSNSSFDRGEIVKI"
     gene            2566..>3236
                     /locus_tag="PLQ70_11145"
     CDS             2566..>3236
                     /locus_tag="PLQ70_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HQE35216.1"
                     /translation="MARFELKLPKMGESVAEATITNWLKQVGDKIEADEAVLEVATDK
                     VDSELPSEVSGILVEQLFGKDDMVQVGQTLAIIETEEHSVSETPKQEVVVPEVAAAIE
                     KSIEVAKESLVVPTNFSDSEKFFSPLVKNIAKEEGISLAELENITGSGKEGRVTKEDI
                     LSFVESRKSKVESEGVKEVEPTPISIPTPIKTIQHPAPNTQHPAPISLNGGDEIIEMD
                     RMRKL"
BASE COUNT         1178 a          487 c          616 g          955 t
ORIGIN      
        1 caattttagt aatgagcagt ttgttcttat tggctactct ttttgccagt tgtgaagcaa
       61 caaaaaatgc aaataacact caaaagggag caggaattgg agctgctgca ggagcgctta
      121 ttggagggat tatagggaat aattcaaaaa ttggaactgc tggaggcgca cttatcggag
      181 cagctattgg tggaggttct ggagctttga ttggaaataa aatggacaaa caagctagag
      241 aaattgacca agcattacca ggagctgatg tagagagagt aggcgaagga attcgcttgg
      301 tgttgaaaga agatgctgtt cgttttgata cgggtaaagc tacattgacc acacaagcca
      361 aagccaattt agacaaattg gttccagtgt ttaaagatta tgccgacacc aatatcgaaa
      421 tttatggtta tactgacagt acaggaagtc cagaattcaa tttgacactt tcgcaaaaaa
      481 gagcagaatc ggtgaaagat tatttagttt caaaaggctt gctagtttct cgttttaaaa
      541 caacaggttt aggaattgca gatccaattg caaccaatga cactgccgaa gggagaactc
      601 aaaaccgtcg tgttgaattt gctattatgg ccaatgacaa aatggtgcaa gacgccaaaa
      661 aagaagcagg tcaataataa agactcaatt attaaatacc cagaaagctg tttcagattt
      721 gaagcagctt ttttgttttg gtttattttg tatatatttg aggtttaaaa tgatttcgaa
      781 aatggtaaat tcaaagaaac attcgattga taaaaaacag gattctgttc aggagcctac
      841 aaccgattat aaagcaactt tcaaagaaaa gctagatcca agtaatacag atgagcaaga
      901 aattttgttg caaaaattat tagcaaaagg tttggaggag agtgaaatgg gaataggtat
      961 ttctaatgaa gaaatgaaaa ggattaccaa attaaaatat cctttcttga aatgatttat
     1021 agaattgaat ggaaatcaat tgcaactcgc acctattttg gagaaataaa tttcatatta
     1081 tcaaaatgga atgaagcaga agttcagaaa tttgaagatt tagtttacga ttttcttgaa
     1141 atagtatcta aaaacccaaa aacaggaatt tataaaccca aaaataattg ttactcattg
     1201 gtgatttcaa aacagaccac tttgtattat aaaatcattg aagataaatc tcaaatcgac
     1261 ttgattttat tttggaataa taaacaaaat ccagacgact taattaagtt gctataatat
     1321 aatcctcaaa aatgcaacta tcagtcataa tcctcaacta taatgtgcgt tacttcttgg
     1381 aactttgtgt ttcaagtgtt caaaacgcta tacaaaacat cgatgccgaa attattgtcg
     1441 ttgataacaa ctctcaagac gatagttgtg caatgatgaa acagcgattt ccgaacgtaa
     1501 aactcatcga aaacaaagaa aattcaggtt ttccaaaagg gaataatatt ggtgttgcaa
     1561 ttgccaatgg agaatatatt tgtattctaa accccgatac ggttgttgcc gaagatactt
     1621 ttacgaaagt attggctttt gctaaaaagc aagaagattt aggaattgtc ggggtgaaac
     1681 tcatcgatgg aacaggaaat tttctacccg aaagcaaacg tggaattcca actccttttg
     1741 tggcttttac caaaattaca ggtttgtata aaatcttttc aaagagtttt ggcaaatatt
     1801 acgctcaaca tttatccgaa aatgaaactg gaaaagtgga aattctcgtg ggtgctttta
     1861 tgctaatgaa acgggattta tacaatgaaa ttggtggttt tgacgaaaaa tgttttatgt
     1921 attcagacga tatcgatttg tcctatatgg ttttgcaaaa gggaaaatca aattattatt
     1981 ttcacgaaac ttctgtcata cattacaaag gggagagcac tttcagagac ggaacttata
     2041 tgaagcgatc tcgtgaagcg atgaattttt tctacaaaaa gcattttaaa gtttcctttt
     2101 tgttttctgt ttttatggaa attggaattg tattcttttc attcgcaaaa atgttttggg
     2161 gcaatccaaa atcaaaatta aagcctaaca attatattct tgtttcaaat aatctgattt
     2221 tgaaagaaaa gttagaaaac agtttagcta aaccaatcca acttcaggat atttcacatt
     2281 caattcagga aaattttaca ggagaaatta ttttagacca aaatcaattc aatttcaaaa
     2341 ctataattaa cttttttgaa gtgcataaaa ataaagggtt tacctttaaa attcttcccc
     2401 aaaactccca ttttgttatt ggtagtaaca gtagttttga tagaggtgaa atagtgaaaa
     2461 tttagaattt taggtttcgt gtaaatttgt gaaattcatg gctgaaactg aaaataatca
     2521 ctaattttgc agtcacgaaa tcaaaatcac cttttaggaa atactatggc aagatttgaa
     2581 ttaaagcttc caaaaatggg agaaagtgta gcagaagcaa ccatcaccaa ttggttgaag
     2641 caagtgggag acaaaataga agcagacgaa gctgttcttg aagttgctac tgacaaagtc
     2701 gatagtgaat tgccaagcga agtatcagga attttagtcg aacaattgtt tggcaaagac
     2761 gatatggttc aagtaggaca aacccttgct attattgaaa ctgaagaaca ttcagtttcg
     2821 gaaactccaa aacaagaagt agttgttcct gaggttgcag cagctatcga aaaatcgata
     2881 gaagttgcta aagaaagtct tgttgtacca accaattttt ctgattctga aaaattcttt
     2941 tctcctttag taaaaaatat tgctaaagaa gaaggtattt ctcttgccga attagaaaat
     3001 attactggtt caggcaaaga aggaagagtg actaaagaag atattttgag ttttgttgaa
     3061 agtcgaaagt cgaaagtcga aagtgaagga gtaaaagagg ttgaaccaac accaatttct
     3121 attccaaccc caataaaaac tattcagcac ccagcaccca acacccaaca tccagcacct
     3181 atttctctaa acggaggcga cgaaattatc gaaatggaca gaatgcgcaa gctgat
//
LOCUS       DAOSNN010000272         3232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_29839_length_3232_cov_4.873780, whole genome shotgun sequence.
ACCESSION   DAOSNN010000272 DAOSNN010000000
VERSION     DAOSNN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29839_length_3232_cov_4.873780"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2353)
                     /locus_tag="PLQ70_11150"
     CDS             complement(<1..2353)
                     /locus_tag="PLQ70_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HQE35217.1"
                     /translation="MKLKSKITAICFGIVSFASTAQTKSELKLWYDKPATIWNEALPL
                     GNGRLGAMVFGDPAVERLQLNEETIWAGSPNTNAHTKSIDALPKVRKLVFEGKFDEAQ
                     ALATQDIMSQTNDGMPYQTFGSTYISFPGHQKYTNYYRDLNIENATAKVKYTVNGVEF
                     TREILSSFSDQVIVVKLSASQPGQITCNVFMNSPIDKTVPSTEGNQIILSGIGTNFEG
                     VKGKVEFQGRIEAKNKGGEVSASNGILSINKADEVTLYISIATNFKNYQDITEDEVAK
                     SKLYLEQAITKDFETIKKAHVAYYQKFFNRVSFDLGKNDLVSKPTNERIRDFSKQFDP
                     QLASLYFQFGRYLLISCSQPGGQPANLQGIWNDMITPPWDSKYTTNINAEMNYWPAEV
                     TNLSEMHEPFIEMAKDLSVTGAATAKMMYNANGWVLHHNTDIWRTTAPVDSAASGMWP
                     TGGAWISQDLWERYLYTGDIKYLKEIYPIMKGAADFFLDFMIMDPNTGYLVVVPSVSP
                     ENVHAGSNGKSTIASGTTMDNQLVFDLFTHVIEASKLVAPDADYAKKVNAALAKMAPM
                     KIGKHSQLQEWQEDWDNPEDIHRHISHLYGLFPSNQISPFKTPELIEAAKTTLKQRGD
                     ESTGWSMGWKVNYWARLLDGNHAYKLLQDQMHLVTAEQKKGGGTYPNMLDAHQPFQID
                     GNFGCTAGIAEMLMQSYDDAIQLLPALPSVWAAGSIKGLVTRGGFVIDMTWKNNKVST
                     LKVYSKIGGNCRLRLSTTLTAGKGISLKKAKGKNPNPLFYDVTI"
     gene            complement(2536..>3232)
                     /locus_tag="PLQ70_11155"
     CDS             complement(2536..>3232)
                     /locus_tag="PLQ70_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HQE35218.1"
                     /translation="FPEETHRNSIASTVQVLSGIRLLDYLSTIKNADVSRVGVTGGSG
                     GGSHTMFLAALDDRITVSAPVVMVSSHFSGGCPCESGRGIHLCGNGTNNAEISAMMAP
                     KPQLIVSDGKDWTLAVPELEFPFIQRTYELYGNKDLVENAHFAKEGHDFGVSKRMALY
                     PFMAKYLGLDLNKVKNAKGEIDESTCVIEPYDKLYVFGNKAENLPKNALKDINKLYEM
                     FGEKNLKVYEVKK"
BASE COUNT          860 a          717 c          597 g         1058 t
ORIGIN      
        1 tgatagtcac gtcataaaac aacggatttg gatttttccc ttttgctttt ttaagcgaaa
       61 ttccctttcc ggctgtcaaa gtagttgata atcgcaatcg gcagtttcct ccaatttttg
      121 aatacacttt caaagtcgaa actttgttgt ttttccaagt catgtcaatt acaaaaccac
      181 ctcttgttac taaccctttg atgcttccgg ctgcccaaac gcttggcaaa gctggcaata
      241 attgaatcgc atcgtcataa ctttgcatca acatttcggc gattccggcg gtgcaaccaa
      301 agttcccgtc aatttgaaac ggttggtgtg catcgagcat atttggatag gttccaccac
      361 cctttttttg ttcggcagta accaaatgca tttggtcttg caataattta tacgcgtgat
      421 ttccgtccaa caatcttgcc caataattca ctttccaacc cattgaccat cctgtagatt
      481 catcacctct ttgtttcaaa gtagttttgg cggcttctat caattctgga gttttgaaag
      541 gcgaaatttg gttgctcgga aacaatccat acaaatgaga aatgtgtcgg tgaatgtctt
      601 cagggttgtc ccaatcttct tgccattcct gcaattggct gtgttttccg attttcattg
      661 gagccatttt agccaaagct gcatttactt ttttggcata atctgcatca ggagcaacca
      721 attttgaagc ttcgataacg tgggtaaaca aatcaaaaac caattgattg tccatcgtgg
      781 taccagaagc aatggtcgat tttccattgc tgccagcgtg tacattttca ggagaaacgg
      841 atggaaccac aaccaaataa cccgtgtttg gatccataat cataaaatcc aagaagaaat
      901 cggcagcgcc tttcataatt ggataaattt cttttaaata ttttatatct ccagtgtaca
      961 aatatctttc ccataaatcc tggctaatcc aagcgccacc cgtaggccac attcctgatg
     1021 cggcagaatc tactggggca gttgtacgcc aaatatcggt gttgtggtgc aagacccaac
     1081 cattggcatt gtacatcatc ttggcagttg ctgctcccgt aacgctcaaa tcttttgcca
     1141 tttcaataaa aggctcgtgc atttccgaaa ggttggtcac ctcggccggc caataattca
     1201 tctcggcatt gatgtttgtc gtgtatttac tgtcccaagg aggcgttatc atatcgttcc
     1261 aaattccttg cagattggct ggttgacctc caggctgtga acacgaaatc agtaaataac
     1321 gtccaaattg gaagtacaaa ctcgccaatt gcggatcaaa ttgtttggag aaatcccgaa
     1381 ttctttcgtt tgtaggtttt gaaaccaaat cgttttttcc taaatcgaat gaaaccctgt
     1441 tgaagaattt ttggtaataa gctacgtgtg ctttttttat agtttcgaaa tcctttgtaa
     1501 tggcttgttc caaatacaat ttgcttttcg ccacttcgtc ttcggtaatg tcttgataat
     1561 ttttaaaatt ggtggctatc gaaatgtaaa gcgttacctc atcggctttg ttgatgctaa
     1621 ggattccgtt gcttgccgaa acttcgcctc ctttattttt ggcttcaatt cttccttgaa
     1681 attccacttt cccttttacg ccttcgaaat tagttccaat tcccgaaagg ataatttggt
     1741 tgccttcagt cgaaggaacc gttttgtcaa tcggactgtt cataaaaaca ttacaggtaa
     1801 tttgccccgg ctgactcgcc gacaatttta ccacaatcac ctgatctgaa aacgagctca
     1861 atatttctct tgtaaattct actccattta cggtgtattt cacttttgcc gtggcatttt
     1921 caatattcaa atcacgataa tagttcgtgt atttttgatg tcccggaaac gaaatatagg
     1981 tacttccaaa agtttggtac ggcattccat cattggtttg cgacataata tcttgggttg
     2041 ccaaagcctg tgcttcatca aatttccctt cgaaaaccaa ctttcttact ttaggcaaag
     2101 catcgattga ttttgtatgc gcattcgtgt ttggcgaacc tgcccaaatg gtttcttcgt
     2161 tcaattgcaa acgttccaca gcgggatctc caaaaaccat tgctcccaat cggccgttac
     2221 ccaaaggcaa agcttcattc catattgttg cgggtttgtc gtaccacaat tttagttcgc
     2281 ttttggtttg cgctgttgag gcgaaggaaa caattccgaa acagattgcc gtaattttac
     2341 tttttaactt catattttat tttttttgac acgaattcac gaattgaatt taattcggga
     2401 ttgtgtttaa tttctcaaat ttatttagcc acagattaaa cggattattt ggattttttt
     2461 ttaatattta aataatctgt gcgaatcatt ttaatctgtg gcagaaaaaa aattcgtgaa
     2521 tttgtggcta tctctctact tttttacctc gtaaacctta agattttttt ctccgaacat
     2581 ttcatataat ttgttgatgt ctttcaaggc atttttaggc aaattttcgg ctttgttgcc
     2641 aaaaacatat aatttgtcgt aaggttcaat cacacaagtc gattcatcga tttcgccttt
     2701 ggcattcttc actttattca aatccagacc taaatatttc gccataaacg ggtacaaagc
     2761 catacgtttt gaaacgccaa aatcgtgccc ttccttggca aaatgggcgt tttctaccaa
     2821 atctttattg ccgtacaatt cataagttct ttggataaaa ggaaattcca actctggaac
     2881 ggccaatgtc cagtcttttc catcggaaac aatcaactgt ggttttggcg ccatcatcgc
     2941 ggaaatttca gcattgttgg ttccgtttcc gcaaaggtga attccgcgac cgctttcgca
     3001 aggacatcct cccgaaaaat gcgaagacac cataacaacc ggagcagaaa ccgtaattcg
     3061 gtcgtccaaa gcagccaaaa acatcgtgtg cgaacctccg ccggaaccgc ccgtaacgcc
     3121 cactcgcgaa acatcagcat ttttgatggt ggacaaataa tccaacaatc gtattccgct
     3181 caagacctga acggttgagg caatgctgtt tcggtgcgtt tcttcgggga at
//
LOCUS       DAOSNN010000273         3210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_30070_length_3210_cov_4.413312, whole genome shotgun sequence.
ACCESSION   DAOSNN010000273 DAOSNN010000000
VERSION     DAOSNN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30070_length_3210_cov_4.413312"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            627..2573
                     /gene="thrS"
                     /locus_tag="PLQ70_11160"
     CDS             627..2573
                     /gene="thrS"
                     /locus_tag="PLQ70_11160"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQE35219.1"
                     /translation="MIKITLPDGSVREFDSGVTPMEVAKSISEGFARNVISASFNGTI
                     VETTTPLTTDGSLILYTWNDEGGKKAFWHSTSHVMAQVLEEMYPGIKLTLGPAISNGF
                     YYDVDFEDQKITDADFKKIEDRVLEISREKHEFKLRPVSKAEALEVYKDNVYKTELIS
                     NLEDGTITFCDHSTFTDLCRGGHIPNTGIIKAMKIMSVAGAYWRGDEKNKQLTRVYGV
                     SFPKQKDLTEYLELLEEAKRRDHRKLGKELELFAFSSKVGQGLPLWLPKGAALRDRLE
                     QFLKKAQKKAGYEQVVTPHIGQKELYVTSGHYAKYGADSFQPINTPAEGEEFLLKPMN
                     CPHHCEIFNTRPWSYKDLPKRYAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCTPD
                     QLDQEFKNVIDLVLYVFGSLGFENFTAQISLRDQENRDKYIGSDENWEKAENAIINAA
                     ADKGLKTVVEYGEAAFYGPKLDFMVKDALGRSWQLGTIQVDYNLPERFELTYKGSDDQ
                     LHTPVMIHRAPFGSMERFIAILIEHTAGKFPLWLMPEQAIILSLSEKYENYAKKVLSL
                     LENHEIRALIDNRNETIGKKIRDAEMQKIPFMLIVGEEEEKNGTISIRRHGQEGKGNI
                     TVTIEEFAAIVNEEISKTLKVFTV"
     gene            2596..3147
                     /gene="infC"
                     /locus_tag="PLQ70_11165"
     CDS             2596..3147
                     /gene="infC"
                     /locus_tag="PLQ70_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQE35220.1"
                     /translation="MRSNRGYQPRVEKKDAHRINNHIRGVQEVRLVGENIEPGVFKLS
                     EALRLADEFELDLVEISPNAEPPVCKIMDYKKFVYMQKKREKELKAKSTQIVVKEIRF
                     GPQTDEHDYEFKRKNAEKFLKEGSKLKAFVFFKGRSIIYKDQGQILLLRLATDLEEFG
                     KVEAMPVLEGKRMIMFIAPKKKK"
BASE COUNT         1059 a          539 c          716 g          896 t
ORIGIN      
        1 attgtttgcc agtatttcaa atgaacgtct gcgtcattgc gggtgcaaaa gtagccactc
       61 ttttcgatta aacaataccc ttttcaatct ttttttaaac tattttttaa gtgattggtt
      121 tttcgtgttt tacaacagca ctttttttgg gtcttgtcat ttttccgtag ctgtttttga
      181 ggatttgttc ggttttagtg cgttttgagc gcgcttgaat tgaaatggaa cgccgataac
      241 agcgattcgc taaagcaaaa tcagcgatta aaaaggtttt ttgttcttca atagtctaaa
      301 aaaacctctc accgacccgc tccaaggaga gaaaaacgtg acgcaaggag tatttgtgga
      361 gcaatgttgt cgattattgt ccaaaacagg aaagttcttt ttaaaaagtg tacttatata
      421 taaggagaag tgctttatag aaaccaacga tctagccctg atggtagcgg catcctcttg
      481 tgccggggtt cggcacaaga gatatagcgg acagcaggat taagctcctg aaaaaaatca
      541 gtcttatata taatgtataa acttgtactt atatattgta tgtattttag taatgcgcta
      601 tatttgcatt cacaaaatta aaaacaatga ttaagattac tttgcccgac gggtcagtta
      661 gagagtttga ttcaggcgta acgccaatgg aggttgccaa aagcattagt gaaggatttg
      721 ccagaaacgt gatttcggct tctttcaatg gtacaattgt agaaaccacg actcctttga
      781 cgacggacgg cagtcttata ttatatactt ggaatgacga aggaggcaag aaagccttct
      841 ggcattcgac ttcgcacgta atggcacagg ttcttgaaga aatgtatccg gggattaaac
      901 taacccttgg gccagcaatc tcgaatggat tttactatga cgtggatttt gaagaccaaa
      961 agattacgga tgccgatttc aaaaagatag aagaccgtgt cttggaaatt tcaagagaga
     1021 aacacgaatt caaattgcgt cctgtttcta aagccgaagc tttggaagtt tacaaagaca
     1081 atgtatataa aaccgaattg atttcgaatc tggaagacgg aacgattact ttttgcgacc
     1141 actctacttt caccgattta tgtcgcggtg gacatattcc aaatactgga atcatcaagg
     1201 cgatgaaaat tatgagcgtt gccggtgctt actggcgtgg tgatgaaaaa aacaaacagc
     1261 ttactcgtgt ttatggtgtt tcgtttccga agcagaaaga cttgacggaa tacctggaat
     1321 tgttggaaga agccaaacgc agagaccaca gaaaactagg aaaagaattg gaattgttcg
     1381 cattttcgtc aaaagtgggt caagggttgc ctttgtggtt gcctaaagga gccgctttgc
     1441 gtgacaggtt agaacaattt ttgaaaaaag cacagaaaaa ggcgggttac gaacaagtag
     1501 tgacgcctca cattggacaa aaagaattat acgtaacttc tgggcattat gctaaatatg
     1561 gagcggatag ttttcagccg ataaacactc ctgccgaagg cgaagagttt ttattgaaac
     1621 ctatgaactg tcctcatcac tgtgaaatct tcaacacaag accttggtca tacaaagatt
     1681 taccaaaacg ttatgctgaa tttggaacgg tttatcgcta tgagcaatca ggcgaattgc
     1741 acggtttaac tcgtgttcgt ggttttactc aagatgacgc tcatattttt tgtacgcctg
     1801 accaattgga tcaagagttc aaaaatgtga ttgatttggt tttgtatgtt tttggctcgt
     1861 tgggattcga gaacttcaca gcacaaattt cgttgcgtga ccaagaaaat agagacaagt
     1921 atattggtag cgatgaaaat tgggaaaaag cagaaaacgc cattattaat gctgctgccg
     1981 ataaaggatt gaaaactgtt gtagaatatg gcgaagcggc cttctatggt ccgaagttgg
     2041 attttatggt aaaagatgct ttagggagaa gctggcaatt aggaacaatt caggtggatt
     2101 acaacttgcc ggagcgtttc gaattgacat acaaaggctc tgatgatcaa ttgcatacac
     2161 cagtaatgat tcacagagca ccatttggat cgatggaacg ttttatagct attttgatcg
     2221 aacacacggc aggaaaattc ccgctttggt tgatgccaga acaggctata atattgtctt
     2281 tgagcgagaa atatgaaaat tatgcaaaaa aagttttaag tctgctagaa aatcacgaaa
     2341 ttcgcgccct tattgacaac cgaaacgaga ctatcggaaa gaaaatcagg gatgcagaaa
     2401 tgcagaaaat cccgtttatg ctgattgtag gcgaggaaga agagaaaaac ggcacaatat
     2461 ccatccgtcg tcatgggcag gaaggaaaag gaaacattac cgtcacaatt gaagaatttg
     2521 ctgcaattgt aaacgaagaa ataagcaaaa cactaaaagt atttacagtt taacttaaat
     2581 tataaagtca tagcaataag aagtaacaga ggttatcaac ctcgagtaga aaaaaaggat
     2641 gcccacagga taaacaatca tattcgtggc gtacaagaag taaggctggt aggtgaaaat
     2701 atcgagcctg gagtttttaa actttccgag gcattaagat tggccgacga atttgaactg
     2761 gatttagttg agatttcgcc aaacgctgaa ccgcctgttt gcaaaataat ggactataaa
     2821 aagtttgttt acatgcaaaa gaaacgcgag aaagagctta aagcaaaatc tactcaaatt
     2881 gtagtgaaag aaattcgttt tggtcctcaa actgatgaac acgattatga gtttaaaaga
     2941 aagaatgccg aaaaattcct gaaagaaggt tctaaattga aagcgttcgt tttctttaaa
     3001 ggacgttcta tcatctataa agatcaaggt caaatcttgc tattgagatt ggctactgat
     3061 cttgaagaat ttggaaaagt ggaagctatg ccagttctgg aaggaaagag aatgattatg
     3121 ttcattgctc cgaagaaaaa gaaatagtat tcagtctgca gaacgcagat tgcagaaaat
     3181 ataatgagat tgcttcgcaa gctcgcaaag
//
LOCUS       DAOSNN010000274         3202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_30150_length_3202_cov_3.735303, whole genome shotgun sequence.
ACCESSION   DAOSNN010000274 DAOSNN010000000
VERSION     DAOSNN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30150_length_3202_cov_3.735303"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1986
                     /locus_tag="PLQ70_11170"
     CDS             199..1986
                     /locus_tag="PLQ70_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQE35221.1"
                     /translation="MAHFKENDLPKSKITATSLNKATVIFKYAGSHRWKFYVGLVFLL
                     LTGATALAFPKLMGILIDSVKTKSYDQANDIAILLIVILFLQSICSFFRLSLFVNFTE
                     HTLANLRLSLYSNLVKLPMSFFSQKRVGELNSRISADITQIQDTLTSTIAEFLRQFIL
                     IIGGIILLATESIKLTLLMLSVVPLVAVAAVIFGRFIRKYSKKVQDQVAESQVIVEET
                     MQGISIVKAFANEWYEIARYNGKIKEVVKLAIKGGKYRGYFASFIIFCLFGAIVAVVW
                     FGVRLSISGEMSVGQLISFVLYSTFVGASFGGIAELYAQIQKAIGATERVFELLDETP
                     EKINATQNSHSVQKIQGNVTFNNVGFTYPSRKEMKVLKGVNFTADFGQKIAVVGPSGA
                     GKSTIASLLLRFYDIDSGEILVDGKNIYDYDLENLRGNMSIVPQDVILFGGTIKENIA
                     YGNPDATNDEIILAAKQANALVFIESFPEKFETLVGERGVKLSGGQRQRIAIARALLK
                     NPSILILDEATSSLDSESEKLVQEALEILMEGRTSIIIAHRLSTIRSADQILVLDKGV
                     IAEQGTHQELIALENGIYKNLSHLQFSHS"
     gene            complement(2126..>3202)
                     /locus_tag="PLQ70_11175"
     CDS             complement(2126..>3202)
                     /locus_tag="PLQ70_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HQE35222.1"
                     /translation="NEPLVRIGTAVTNFALKASLPVEGLIRATVFDHFCGGVNEDDSL
                     KVVDKLFTKGVSSVLDYSVEGKEEEEQFDAVLEKTLKLIDFAKEKKSIPFAVFKPSGF
                     GRINLYEEVSLGKKLTPAESDEWDRVVARFEKVCKASYDSDVALLIDAEESWMQDSVD
                     NLVEEMMQKYNKEKPIVFNTLQMYRWDRLDYLKKVHEKAKTEGFFIGMKLVRGAYMEK
                     ENERAIEKNYPTPICASKEATDENYNASVKYMLDHIDMMSIFEGTHNEESTYYLMDLM
                     EKKGIQSTDNRIWFGQLYGMSDNISYNLADSGYNVAKYLPFGPVKDVMPYLIRRAEEN
                     TSVAGQTSRELAMIKAERNRRKGK"
BASE COUNT         1038 a          577 c          524 g         1063 t
ORIGIN      
        1 cccgaccccc tcggtgtaaa cgaggtgctc tgaaccagct gagctaatcg ccctaattgt
       61 gggtgcaaat atacacttag atattggatt cgcaaacaaa atttaaaaaa atataatttt
      121 aatttgaaat gaaataaaac atatttttat cattacttca ggatagtatt atatttgtaa
      181 aaaaacaatt aaatttaaat ggcacatttc aaagaaaatg atttaccaaa atcaaaaata
      241 acagcaacat ctctaaacaa agcaacagtt atttttaaat atgcaggaag tcatcgttgg
      301 aaattttatg taggtctagt ttttctactt ttaaccggcg caactgcttt agctttcccc
      361 aaattaatgg gaattctaat tgatagtgta aaaaccaaaa gttacgatca agctaatgat
      421 attgctatat tacttatagt aatattgttt ttgcaatcta tatgttcctt ttttaggctt
      481 tctttatttg tcaattttac ggaacatact ttagcgaatc ttcgattatc attgtacagt
      541 aacttagtaa aactgccaat gtcattcttt tcacaaaaac gtgttggaga actaaatagt
      601 agaataagtg ccgatattac tcaaattcaa gatacattaa catctacaat tgccgaattt
      661 ttaagacaat ttattttaat tattggcggc atcattttgt tagccaccga aagtataaag
      721 cttactttat tgatgctttc agtagttcca cttgtggcgg tagctgcggt tatatttgga
      781 agattcattc gaaaatactc caaaaaagta caagatcaag ttgcagaaag tcaagttatt
      841 gttgaagaaa cgatgcaagg tattagtatt gtaaaagctt tcgccaatga atggtatgaa
      901 attgcccgat acaacggaaa aattaaagaa gtagtaaaac tagctatcaa aggaggaaaa
      961 tacagaggct actttgcttc cttcatcata ttttgcctat ttggagctat cgtagctgtg
     1021 gtttggtttg gcgtgagatt gagtattagt ggcgaaatga gcgtgggaca attaatctct
     1081 tttgtactat attctacatt tgttggggca tcttttggag gaatagctga actttatgct
     1141 caaattcaaa aagcaattgg agccacagag cgtgtttttg aattattaga cgaaactcct
     1201 gagaaaatta atgctacaca aaattcgcat tccgttcaaa aaattcaagg caatgtcact
     1261 ttcaataatg ttggattcac ctacccttct cgaaaagaaa tgaaagtatt gaaaggagta
     1321 aatttcacag ctgattttgg tcaaaagatt gctgttgttg gtccaagtgg tgctggaaaa
     1381 tctactatag cttctttatt attgcgcttt tacgacattg acagtggaga aatattggta
     1441 gatggaaaaa atatctacga ttatgattta gaaaatcttc gtggtaatat gagcattgtt
     1501 cctcaagacg ttattttatt tggcggaact attaaagaaa acattgctta cggaaatcct
     1561 gatgcaacaa atgatgaaat tattttggct gcaaaacagg ccaatgcttt ggttttcata
     1621 gaaagtttcc ctgaaaaatt tgaaactttg gttggagaaa gaggcgttaa actttctgga
     1681 gggcaacgcc aacgtattgc tattgcaaga gcattattaa aaaatccaag tattttgatt
     1741 ctggacgaag ctacttcttc attagatagc gaaagcgaaa aactggttca ggaagcttta
     1801 gaaatcttga tggaaggaag aacgagcatc attattgcac accgcctttc gaccattcga
     1861 agtgcagacc aaattttggt tttagataaa ggagtaattg ctgagcaagg aactcatcaa
     1921 gaattgattg ctttagaaaa cggtatttat aaaaacctga gccatttgca gtttagtcat
     1981 tcttaattta tagacgctga tgaaactgat tcgcaaaagc gaaaacgcaa ataaaaactg
     2041 attagcattt tatttatgca actaaaatca gtttttatcc gcgtctttac gaagtaaatc
     2101 cgtgttatcc gtgttccaat aacgactact tcccttttct cctatttctc tccgctttta
     2161 tcattgctaa ttcacggctg gtttgtcccg ctaccgaagt attttcttcg gcacggcgaa
     2221 ttaagtaagg catcacatct tttacaggac caaaaggcaa atatttagca acattataac
     2281 cactatcggc caaattataa ctgatattgt cgctcatacc atacaattga ccaaaccaaa
     2341 ttctattgtc tgtcgattgt attccttttt tttccattaa atccatcaaa taataagtac
     2401 tttcttcgtt gtgtgttcct tcaaaaatag acatcatatc aatatgatct aacatatatt
     2461 ttactgatgc attataattt tcatcagtag cttctttgga agcgcaaatt ggagttggat
     2521 aatttttttc aatggcacgt tcattttctt tttccatata agcaccacga actaatttca
     2581 ttccaatgaa aaaaccttct gttttagctt tttcgtgcac ttttttcaaa taatccaatc
     2641 gatcccaacg atacatttga agcgtattga aaacaattgg cttctctttg ttgtacttct
     2701 gcatcatttc ttcaaccaaa ttatcgaccg aatcttgcat ccaactttct tcagcatcaa
     2761 tcaataaggc cacatcacta tcataagagg ctttgcacac tttttcgaaa cgtgccacta
     2821 ctctatccca ctcatcagat tctgctggtg ttaatttttt tcctaaacta acttcttcat
     2881 acaaattgat acgcccaaaa cctgatggtt tgaacacagc aaacggaatt gatttttttt
     2941 cttttgcaaa atctatcaat tttaaagttt tttctaaaac cgcatcaaat tgttcttctt
     3001 cctccttgcc ttccacagaa taatctaaaa ctgatgaaac tcctttagta aataatttgt
     3061 ccacaacttt caagctgtcg tcctcgttta ctcctccaca aaaatggtca aaaacagtgg
     3121 cacgaatcaa tccttctaca ggcaaacttg ctttgagagc aaaatttgtt acagcagttc
     3181 caattcgaac aagtggttca tt
//
LOCUS       DAOSNN010000275         3170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_30551_length_3170_cov_4.406100, whole genome shotgun sequence.
ACCESSION   DAOSNN010000275 DAOSNN010000000
VERSION     DAOSNN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30551_length_3170_cov_4.406100"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..341
                     /locus_tag="PLQ70_11180"
     CDS             12..341
                     /locus_tag="PLQ70_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35223.1"
                     /translation="MFLIVTKYLIPKGYRGLAIFPFVFVKYRLDKGNSVFVNHERIHL
                     RQQLELLIIPFFIWYFLEFCLRWIQFKNFDLAYRNISFEREAYANEKDLDYLKRRSFW
                     NFLKYVR"
     gene            387..1292
                     /locus_tag="PLQ70_11185"
     CDS             387..1292
                     /locus_tag="PLQ70_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQE35224.1"
                     /translation="MQEIQTQEIKLSHKSITLFIRREDLIHPFISGNKFRKLKYNLDQ
                     AKSENHDTLLTFGGAFSNHIAAAAYAGAAYRFKTIGIIRGDELESKIDQNPTLKFAKE
                     CGMQFEFVTREEYRHKTEVFFLENLKQKYGDFYLVPEGGTNEFAVKGCEEILTKEDSK
                     YDFVCTAVGTGGTISGIINSSLPHQKVLGFPALKGDFLNDEIRKFAHKDNWELITDYH
                     FGGYGKVSSELIEFINQFYVVNQVPLDPIYTGKMVFGVMDLINKNYFPEHSKILLIHT
                     GGLQGIKGMNINLKNKHLPIINIDV"
     gene            1285..2106
                     /locus_tag="PLQ70_11190"
     CDS             1285..2106
                     /locus_tag="PLQ70_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HQE35225.1"
                     /translation="MCKKIVLIAFILILVGCSSSKSVSVTTKSGNSRYNKTNSTPNKN
                     SQSNSRALVTSDLVNGYVFHYKDIAMSNMKKYGIPASIILAQGILESGSGQSNLAVTA
                     NNHFGIKCYTDWKGETIHQDDDSAQECFRKYKNPEESFQDHADILSKRNRYASLFNLK
                     KGDYKAWARGLKAAGYATDPNYPEKLIGYIERFHLDQYDNMVMGKDYVLDENQYVKPK
                     PKVVSNTNSNQYIVAKGDTLYSISKKFNIAIEELKRQNNLNDNAISIGQSILIQK"
     gene            2112..>3170
                     /locus_tag="PLQ70_11195"
     CDS             2112..>3170
                     /locus_tag="PLQ70_11195"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HQE35226.1"
                     /translation="MLYQRSSQLFAEAEKVIPGGVNSPVRAFKAVGGTPIFVKSAKGA
                     YLLDEDGNQLIDYINSWGPMILGHAFQPVVDAVIEKAKLGTSFGMPTELETQIAALAV
                     KMVPNIDKIRFVNSGTEACMSAIRLARGCTKRDKIIKFAGCYHGHSDSFLIQAGSGAI
                     TFGSPNSPGVTAGTAKDTLLAKYNDLENVSALIEANKDEIAAIIVEPVAGNMGCIPPN
                     KGFLEGLRQLCTDNGILLIFDEVMTGFRLSAGGVQELFNIKADIVCFGKVIGGGLPVG
                     AFAARNEIMNYLAPLGPVYQAGTLSGNPLAMAAGLAMLQALDSDREIYKRLEEKTAYL
                     AAGIDKVLKTNKVDFTINR"
BASE COUNT         1084 a          511 c          614 g          961 t
ORIGIN      
        1 ctttataaca aatgtttcta attgttacca aatatttaat tccaaagggc tatcgcggat
       61 tagctatatt cccgtttgtg tttgtgaaat atcgattgga taaagggaat tctgtttttg
      121 ttaatcacga gcgaattcac ttgcggcaac aattagaact gttaatcatt ccttttttta
      181 tttggtattt tcttgagttt tgtttgcgat ggattcaatt taaaaatttt gatttggcct
      241 atagaaatat cagttttgag cgtgaggctt atgccaatga aaaagacctt gactatttaa
      301 agcgaaggtc tttttggaat tttttgaaat acgttcgtta gttacctata ttcagtattg
      361 tatctttgtc taaaataata cctgttttgc aagaaattca aactcaagaa ataaaattat
      421 cgcacaaatc aattaccctt tttataagaa gggaagatct cattcatcct tttatttctg
      481 gaaacaagtt taggaaatta aaatacaatc tcgatcaggc aaaatcagag aatcacgata
      541 cgctactcac ttttggcgga gcattctcca atcacattgc cgctgccgct tatgcaggtg
      601 ccgcgtatag attcaaaacc ataggaatta ttcgtggaga tgaattagaa agtaaaatag
      661 accaaaatcc aaccctaaag tttgctaagg aatgcggaat gcaatttgag tttgtcaccc
      721 gagaagaata tcgtcataaa accgaagtct tttttttaga aaatttaaag caaaaatatg
      781 gtgactttta tctagttcca gaaggtggaa ccaatgaatt tgcagtcaaa ggatgcgaag
      841 aaattctaac taaagaagat tcgaaatatg attttgtgtg tacagccgtt ggaacaggcg
      901 gaacgatttc agggataata aacagcagtt tgccacatca aaaagttttg ggctttcctg
      961 ccctgaaagg tgatttttta aacgatgaaa ttcgtaaatt tgcccacaag gacaattggg
     1021 aattaattac agactatcat tttggtggct acggaaaagt cagttccgaa ttaatcgaat
     1081 tcatcaatca gttttatgtt gtaaatcaag ttcctttgga tcccatttat acaggaaaga
     1141 tggtttttgg cgttatggat ctaatcaata aaaattattt tccagaacat tccaaaattt
     1201 tactcattca cactggagga ctacagggaa taaaagggat gaacattaat ttaaaaaaca
     1261 agcatttacc aataataaac atagatgtgt aaaaagatag tacttatcgc cttcattcta
     1321 attttagtgg gttgttcctc ttctaaatct gtgagtgtaa ctacaaaatc aggaaattcg
     1381 aggtataata aaacaaattc caccccaaat aaaaactcac aatccaattc aagagcactt
     1441 gtaacttctg atttggtaaa cggatatgtg tttcattaca aagacattgc aatgagtaat
     1501 atgaaaaaat atggcattcc tgccagtatc attttagctc aaggaattct tgaatcgggt
     1561 tcaggtcaaa gcaatttagc ggtgacagca aacaatcatt ttggaataaa atgctacacc
     1621 gattggaaag gcgaaacgat ccatcaagat gacgattctg cccaagaatg tttcaggaaa
     1681 tataaaaatc cagaagaatc atttcaggat cacgccgata ttctttctaa aagaaaccgt
     1741 tatgcttcct tatttaactt gaaaaaagga gattataagg cttgggcgag aggtttaaaa
     1801 gcagcaggtt atgcaacaga tcctaattat ccagagaaat taattggtta cattgaacgc
     1861 tttcatttgg atcaatatga taatatggtt atgggcaaag actatgtttt ggatgaaaat
     1921 caatatgtaa agccaaaacc gaaagttgta tcaaatacaa attcaaatca atatatagtt
     1981 gccaaaggcg ataccttata ttcgatttcc aaaaaattta atatcgcaat tgaagagttg
     2041 aaaaggcaaa ataatttgaa tgacaatgcc atttcaattg gacaatccat actaattcaa
     2101 aaataaaaca aatgttatat caaagaagta gtcagctttt tgctgaagca gaaaaagtaa
     2161 ttccgggtgg tgtaaattcg cctgttcgtg cattcaaagc ggttggagga actccaatat
     2221 ttgtaaaaag tgccaaagga gcctatttgt tagacgaaga cggaaaccaa ttaatagatt
     2281 atatcaattc ttgggggcca atgattttgg gtcacgcttt tcaacccgtt gtagatgccg
     2341 taatcgaaaa agccaaatta ggaacctcat ttggaatgcc aaccgaatta gaaactcaaa
     2401 tcgctgcttt ggctgtgaaa atggttccca atatcgataa aataagattt gtaaattcag
     2461 gaaccgaagc ttgtatgagt gccattcgat tggctcgtgg atgtactaaa agagacaaaa
     2521 ttatcaaatt tgctggttgt tatcacggtc attccgattc gtttttgata caagccggaa
     2581 gtggcgccat tacttttggt tctcctaata gtcctggcgt aactgcagga acagctaagg
     2641 atacattgct tgccaaatac aatgatttag aaaatgtttc ggctttaatc gaagctaata
     2701 aagatgaaat cgctgccata attgtagaac ctgtggctgg aaatatgggt tgtattcctc
     2761 caaacaaagg ctttctggaa ggtttacgcc aattgtgtac agacaacgga attctattga
     2821 tttttgatga ggtaatgact ggctttcgat tatcagcagg tggagttcaa gaattattca
     2881 acatcaaagc cgatattgtt tgcttcggaa aagtaattgg tggaggtttg ccagtaggtg
     2941 catttgctgc cagaaacgag attatgaatt atttggctcc actaggtcca gtgtatcaag
     3001 cgggaacatt atctggaaat ccattggcaa tggcggcagg attagcgatg ttgcaagcct
     3061 tggattctga tagagaaatt tacaaaagat tagaagaaaa gaccgcttat ttggcagcag
     3121 gaattgataa agtcctaaaa acaaataaag tagattttac catcaataga
//
LOCUS       DAOSNN010000276         3169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_30565_length_3169_cov_3.576108, whole genome shotgun sequence.
ACCESSION   DAOSNN010000276 DAOSNN010000000
VERSION     DAOSNN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30565_length_3169_cov_3.576108"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..517)
                     /locus_tag="PLQ70_11200"
     CDS             complement(128..517)
                     /locus_tag="PLQ70_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HQE35227.1"
                     /translation="MRFHTRKWVKPEDLNPNGSLFGGKLLAWIDEELALYTIIQLENS
                     RIVTKHMSEINFRSSARQGDIIEIGIDVVRFGKTSLTLICEARNMMTRETIITIESTT
                     MVNLGPDGKPKAHGKTEIEYVKDRLED"
     gene            587..988
                     /locus_tag="PLQ70_11205"
     CDS             587..988
                     /locus_tag="PLQ70_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HQE35228.1"
                     /translation="MDQRDVFLKEFRGETIGIVTPQSSADELFQNEVLRPILKLQNDL
                     FIASFINYLGKNRIHFDTFSVEKKLTAIENAIQKDIKFRNALKGMIIALFTIDEYAQY
                     IKNSSSLNKRMMGMLIERLKSQVQLFEVQGI"
     gene            complement(985..2640)
                     /locus_tag="PLQ70_11210"
     CDS             complement(985..2640)
                     /locus_tag="PLQ70_11210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008636165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQE35229.1"
                     /translation="MKSYLTLICFSLLIFYSAKSQNLLDGKAEVFTRQDTLRGSITKE
                     RAWWDVKYYHLDVKVNPADSTISGSNTIRYKVLKSYNRMQIDLQKPMEISKVTQDGKE
                     LKFERDENVFYINLVEPQNVGDIKEVVVFYGGKPKIAVNPPWDGGITWSKDKNGKPFI
                     ASTCQGLGASVWWPCKDHMYDEVDNMLISVNVPKNLTDVSNGRLQSIIDLKDGTKTFN
                     WYVSNPINNYGVNINIGDYVSFSEVFKGEKGDLDCEYYVLRDDLEKAKIQFKDVPRML
                     KAFEHWFGPYPFYEDSYKLVQAPYLGMEHQSCVTYGNGFQNGYLGRDLSGTGWGLKFD
                     FIIIHESGHEWFANNITYKDIADMWIHESFTNYSESLFVEYYYGKEAGAEYVRGTRKG
                     IKNDKPIIGQYDVNNEGSGDMYPKGGNMLHTLRQIVNDDPKWRTILRGLNSTFYHQTV
                     TTKQIEDYLSKAVGLDLNPFFNQYLRAIRIPTFEYKFLKGKLSYRWTNCVPDFDMPVK
                     VTINGKAKWLKPQAEWTNLPQTSKNLKLEVDKDFYVNVLKTES"
     gene            complement(2655..>3169)
                     /locus_tag="PLQ70_11215"
     CDS             complement(2655..>3169)
                     /locus_tag="PLQ70_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQE35230.1"
                     /translation="VASDVIHSYNGTSIEIIDTDAEGRLVLADGLSYLIKNFKPEYII
                     DIATLTGSAMGTFGHECAALFTNNELVSKKLQESGDAIGERLWPLPLWDCYKSDIESE
                     IADVKNYSGKPLAGAITAAKFLEYFTDNHKAWAHLDIAGVAFGNDEFAKSKHATAYGV
                     HLLTKFIENL"
BASE COUNT          979 a          628 c          498 g         1064 t
ORIGIN      
        1 aataaccaaa attttattat tattttaaga gcctcactac ttccctttgc cactttttat
       61 aatgagaaaa agtatttaag aaaaatataa ccctcaaaaa ttatttttga aaacagaatt
      121 gaaacaatta gtcctctaga cggtctttta catattcaat ttctgttttt ccgtgcgctt
      181 ttggcttacc atcaggacca aggttaacca tcgtggtgct ctcgatagta ataatggttt
      241 cgcgtgtcat catatttcga gcttcacaaa tcaaagtaag cgaagtttta ccaaaacgaa
      301 ccacatcaat tcctatttcg ataatatcgc cttgacgggc cgagcttctg aaattaattt
      361 cagacatatg cttggttaca attcttgagt tttccaattg aattatagtg tataaagcca
      421 attcttcgtc aatccaagct aataattttc cgccaaataa actaccgttt gggtttaaat
      481 cttcgggttt tacccatttt ctagtatgaa atcgcatatt gtttttttta tattggcgaa
      541 aataaggttt tctatctctt tttttttatt tttaaagaaa aaaaagatgg atcaaagaga
      601 tgtttttttg aaagaattta gaggtgaaac cattggtatc gtaactcctc aatcctcggc
      661 agatgaatta tttcaaaacg aagtacttcg ccctattttg aagctacaaa atgatttgtt
      721 tatcgcttcg ttcatcaatt atttgggtaa aaatcgaatt catttcgata ctttttcagt
      781 tgaaaaaaaa ttgactgcca tcgaaaatgc gattcaaaaa gacatcaaat tccgaaatgc
      841 cttgaaagga atgattatcg cacttttcac catcgacgaa tatgcgcagt acatcaaaaa
      901 ctcttcaagc ctcaacaaaa gaatgatggg aatgctcatc gaaagattga aaagtcaagt
      961 gcagttgttt gaagttcaag ggatttaaga ctcagttttc aaaacattca cataaaaatc
     1021 cttatccact tccaacttta aatttttaga agtttgtggc agattcgtcc attctgcttg
     1081 aggttttaac cattttgctt ttccgtttat ggtgactttt actggcatat cgaaatcagg
     1141 aacacaattt gtccaacgat aagaaagttt acctttcaga aacttatatt cgaaagttgg
     1201 aattctaata gctctcaaat attgattgaa aaatggattc aaatctaaac cgacagcttt
     1261 actcaaataa tcttcaattt gcttggtggt aacagtttga tggtaaaaag tactgttcaa
     1321 acctcgtaaa atagttctcc attttgggtc atcatttaca atttgacgca aagtatgcag
     1381 catatttcca ccttttggat acatatcacc cgagccttca ttgtttacat catattgtcc
     1441 aatgatgggt ttgtcgtttt taattccttt tcgggttcct ctaacatatt cggctccagc
     1501 ttcttttccg tagtaatatt cgacaaaaag actttctgaa tagttggtga aactctcgtg
     1561 aatccacata tcggcaatgt ctttgtaggt aatattattg gcaaaccatt cgtgacccga
     1621 ttcgtgaatg atgataaaat cgaatttcaa tccccaacct gttccgctca aatctctgcc
     1681 taaatatcca ttttgaaaac cattgccata agtaacacaa ctttgatgtt ccattcccaa
     1741 ataaggcgct tgtaccaatt tgtaactgtc ttcataaaac ggataaggac caaaccaatg
     1801 ttcgaaagct ttcaacattc gtggcacatc tttgaattga attttggctt tttctaaatc
     1861 atctctcaaa acataatatt cgcaatctaa atcgcctttc tctcctttga agacttctga
     1921 aaatgaaaca taatccccta tattgatatt cactccataa ttattaattg ggttcgaaac
     1981 ataccaattg aaggttttgg tgccatcttt caaatcgatt atactttgca aacgaccgtt
     2041 ggaaacatcc gttaaattct tgggaacatt cacgctgatg agcatattat cgacttcgtc
     2101 atacatatgg tctttgcaag gccaccaaac actggcaccc aatccctgac aagtcgaggc
     2161 aataaaaggt tttccgttct tgtctttgct ccaggtaatg ccaccatccc aaggtggatt
     2221 tactgcaatt ttgggcttac caccatagaa aacaacaact tcttttatgt ctccaacatt
     2281 ttgtggttct actaaattga tataaaaaac attttcgtct ctttcgaatt ttaactcttt
     2341 gccatcttgg gttactttgg aaatctccat cggtttttgc aagtcaattt gcattcgatt
     2401 ataggatttc aaaaccttat aacgaatggt attcgaaccc gaaatggtac tatcggcagg
     2461 attaactttg acatcaagat ggtaatattt tacatcccac caagctcttt ctttagtaat
     2521 actgcctctt aaagtatctt gtctggtgaa tacctctgct tttccatcaa gaagattttg
     2581 ggattttgca ctatagaaaa tcagtaagga gaaacaaatt agagttaaat aggatttcat
     2641 ataaataaac ttatttacaa attctcaata aatttagtca ataaatgaac accataagcc
     2701 gtggcgtgtt tgcttttggc aaattcgtcg ttaccaaacg ctactcctgc aatatccaaa
     2761 tgtgcccaag ctttatgatt atcagtgaaa tattctaaaa atttggcggc tgttatggct
     2821 ccagccaatg gttttccgct ataattcttg acatccgcaa tctcactttc tatatcggat
     2881 ttgtagcaat cccaaagcgg caacggccaa agtctttcgc caatggcatc gcctgattct
     2941 tgcagttttt tggaaacaag ttcattattg gtaaacaacg ccgcacattc gtgaccaaaa
     3001 gttcccatcg cacttcctgt taatgttgca atatcaataa tatattcggg tttgaaattt
     3061 ttgattaaat atgacaatcc atcggctaaa accaatcgcc cttcggcatc ggtgtcgata
     3121 atttctatcg aagttccgtt ataggaatga atgacatcac tggctacaa
//
LOCUS       DAOSNN010000277         3134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_31058_length_3134_cov_4.730432, whole genome shotgun sequence.
ACCESSION   DAOSNN010000277 DAOSNN010000000
VERSION     DAOSNN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31058_length_3134_cov_4.730432"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLQ70_11220"
     CDS             complement(<1..243)
                     /locus_tag="PLQ70_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HQE35231.1"
                     /translation="MSKEIEAKIEKIPVLRNVVRPLKKIKLPKLGGLSFYDLLELYGL
                     GIAESAFSYHASAIAFSFFMALFPFALFILNLIPYIP"
     gene            complement(253..1110)
                     /gene="nadC"
                     /locus_tag="PLQ70_11225"
     CDS             complement(253..1110)
                     /gene="nadC"
                     /locus_tag="PLQ70_11225"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HQE35232.1"
                     /translation="MISEKQFNNELQLIIHNAIREDIGDGDHSSLACIPTSATGKAKL
                     IVKEDGIIAGVAFAKMIFEYVDSNLIMETFITDGTAVKKGDVVFHVSGSSQSILKSER
                     LVLNSMQRMSAIATKTKNYVDLLQGTKTQILDTRKTTPGFRACEKWAVKIGGGENHRF
                     ALYDMIMLKDNHIDFAGGITLAIAKTKAYLKENNLDLKIIVEARDLDEIKEILESDGI
                     YRILIDNLDFEDTKKAVALIGDKCQTESSGNINENTIRSYAECGVDFISSGALTHSIY
                     NMDLSLKAF"
     gene            1363..2856
                     /locus_tag="PLQ70_11230"
     CDS             1363..2856
                     /locus_tag="PLQ70_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HQE35233.1"
                     /translation="MKKRKFPSAQTILLIIAAFVALLTWIIPSGKYDSLIYNKSDNTF
                     SLDSKGKTSQIPANQKSLDELSIKIPLKNFTNGAIYKPISIPNTFEKVEANPQGFFEF
                     VQSPVKGIIEASDVIFLVLFIGGLIGIMNLTGAFDAGIFWLSDVLKGREYLLIIFTTT
                     LIAIGGTTFGLAEETLAFIPILIPVFIAAKYDAMVGIACIFLGSSVGSMCSTTNPFAT
                     IIASDSAGINWTTGIYGRIIMFCICTTISIIYILRYAKRIKNDPTKSIIFDQKEEIEK
                     LFGSNKENIQSKLTLRLRIILFIFTASFIIMVIGVSMLDWWFTEMASVFFVGAIGIGF
                     VAKIKENDFVNAFSNGAGELLSVAFIIGIARGVSILMKDGCISDTVLYNTSAITEGMN
                     KGVFVNTLFFIYNGLSFFIPSSSGMAVLSMPIMSPLADTVGIGRETIVNSYLYGMGIF
                     GFINPTGMILATLGIVKIGFDKWLKFVWPLVVVLVLISMVFLTLSVY"
     gene            complement(2873..>3134)
                     /locus_tag="PLQ70_11235"
     CDS             complement(2873..>3134)
                     /locus_tag="PLQ70_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HQE35234.1"
                     /translation="RKAKGKETWFLGAITDENARKSEIKLDFLDKGQKYKAIIYEDAK
                     DAHWQKNPIAYKIRTLEVTNKSKINLNLAPGGGTAISFEPIK"
BASE COUNT          943 a          523 c          527 g         1141 t
ORIGIN      
        1 aggaatatac ggaatgaggt ttaaaataaa caaagcaaag ggaaacaaag ccatgaaaaa
       61 gctaaatgca attgcgctgg catgataaga aaaggcactt tcggcaattc cgagaccgta
      121 caattccaat aagtcgtaaa aagacaaacc tcctaatttg ggtagtttta tcttttttaa
      181 tggacgaacc acattgcgca gtactggtat tttttcaatt tttgcttcta tttctttact
      241 cattttttag atttaaaaag ccttcaaact caaatccata ttataaattg aatgtgtcaa
      301 tgctcctgat gaaataaaat caacaccaca ttcggcataa ctgcgaatag tgttttcgtt
      361 gatgtttcca gaagattctg tttgacattt atctccaatc aaggcaactg cttttttggt
      421 gtcttcaaaa tcaagattat caatcaagat tctataaata ccatcacttt cgagaatttc
      481 cttgatttca tccaaatctc tggcttcgac aatgattttt aaatcgagat tattttcttt
      541 gagataggct ttggttttgg ctatagccaa tgtaattcca cctgcaaaat caatatgatt
      601 gtcctttagc attatcatat catagagtgc aaaacggtgg ttttcgcctc cgccaatctt
      661 cactgcccat ttttcgcaag cacggaaacc aggtgtagtt ttgcgcgtat ctagaatttg
      721 tgttttggtt ccttgcaata aatctacata atttttggtt ttggtagcaa tagcactcat
      781 tctttgcata gaatttaata ctaacctttc ggattttaaa atagattgag aacttcctga
      841 aacatgaaag actacatcgc ctttttttac agctgttcca tcagttataa atgtttccat
      901 tattagattg ctatccacat attcgaaaat cattttggca aaagccactc ctgcaataat
      961 accatcttct ttgactataa gttttgcttt tcctgttgcc gatgttggaa tacaggccaa
     1021 tgaactgtga tcgccatcac caatatcttc acgaatggca ttatgaatta tgagttgtag
     1081 ttcgttgtta aattgttttt cgctaatcat ttttatctaa aatttcaaga tgctaaagta
     1141 tgattttatt tggtgaatta aaaatttgag gtttcccaaa ttcattaaat tacagaatcg
     1201 ttttaatttt ctgaatgttt ttttaagcac ttttgattac atttgatact tgtaagtttt
     1261 cttatctttt aactcacaac aattaagaat tgcggattat gttactaata cgaattttat
     1321 gctgaaaatg agatagaaaa ttatgaatca taaaaaatca atatgaaaaa aagaaaattt
     1381 ccttctgcac aaactatttt actaattatt gcagcatttg tagccctact cacttggatt
     1441 attccttctg gaaaatatga tagtttaatt tacaacaaat cagacaatac atttagcctt
     1501 gacagcaaag gaaaaacaag tcagattcct gccaatcaaa aatcgcttga cgaactttcg
     1561 ataaaaatac ctctcaaaaa ctttactaat ggagccattt acaaacccat tagtattccg
     1621 aatacattcg aaaaagtgga agccaatccg caaggttttt ttgagtttgt gcaatcgcct
     1681 gttaagggaa taattgaggc ttcggatgtt atttttttgg tactttttat tggtggatta
     1741 atcggaatta tgaatcttac aggagcattt gatgctggaa ttttttggtt atcagatgtg
     1801 ctgaaaggga gagaatacct gctaataata tttaccacca ctctaattgc tattggcggt
     1861 accacttttg gcttagccga agaaacctta gcttttatac ctattttaat tccagttttc
     1921 attgctgcta aatatgatgc tatggtggga atagcttgca tttttttagg aagttctgta
     1981 ggttcgatgt gttctactac taacccattt gcgaccatta tagcttctga ttctgctgga
     2041 ataaattgga ctactggaat ttatggaagg attattatgt tctgtatttg tacaaccatt
     2101 tctattattt atattcttcg ttatgcgaaa cgaatcaaga atgaccccac aaaatcaatc
     2161 atttttgacc aaaaagaaga aatagaaaaa ttatttggga gcaacaaaga aaatattcaa
     2221 tcaaaattaa ccctcagatt aagaatcata cttttcatat tcacggcaag ttttatcatt
     2281 atggttattg gagtttcgat gttggattgg tggtttacag aaatggcatc cgtgtttttt
     2341 gttggagcta ttggcattgg ttttgttgcc aaaataaagg aaaatgattt tgtaaatgca
     2401 ttttctaatg gagctggcga attgttgagt gttgctttta tcattggcat tgccagagga
     2461 gtttcgatcc tgatgaaaga tggttgtatt agtgacaccg tactttacaa taccagcgcc
     2521 ataaccgaag gaatgaacaa aggagttttt gtaaacaccc tgttttttat ctataatggt
     2581 ttgtcttttt tcatcccttc ttcatctgga atggcggttt tatctatgcc aattatgtct
     2641 ccattggctg atactgttgg cattggaaga gaaaccatag tcaatagtta tctatatgga
     2701 atgggaatat tcggatttat caacccaact ggaatgatat tagcaacctt aggaatcgtt
     2761 aaaattggct ttgacaaatg gctcaaattt gtatggccat tggtggtcgt attagtctta
     2821 atttcaatgg tatttttaac tctttctgtt tattaattgc agcttgcaat ttttacttta
     2881 tgggttcaaa acttattgct gttcctcctc ctggagccaa attcaaattg atttttgatt
     2941 tattggtcac ctctaatgtt cttattttat aagcaatagg atttttttgc caatgggcat
     3001 cttttgcatc ttcatagata atggctttat acttttgccc tttgtcaaga aagtctaatt
     3061 taatttctga ttttcttgca ttttcatcgg ttattgctcc aagaaaccaa gtttcttttc
     3121 ctttggcttt tcta
//
LOCUS       DAOSNN010000278         3133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_31065_length_3133_cov_5.341780, whole genome shotgun sequence.
ACCESSION   DAOSNN010000278 DAOSNN010000000
VERSION     DAOSNN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31065_length_3133_cov_5.341780"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2165)
                     /locus_tag="PLQ70_11240"
     CDS             complement(<1..2165)
                     /locus_tag="PLQ70_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35235.1"
                     /translation="MLKNILILLAILGFSELHAQESNSLYKTKKILVSNDTIPIEKES
                     LNSSFFKLLDANNQPIDSSFYKINFEKGTLLLNEKLTSKSDSITVQYLKLPEILTKEY
                     RIYDSNKMVSNEASKGSLYTVETISQKKNIPFEGLNTSGSISRGITIGNNQNSVLTSN
                     LDLQITGKLSDKVSLRASLQDNNIPLQDGGYSQKLDQFDNIFMELFSEKWNIRAGDVF
                     LENHKSQFLNFNKKAQGISTSFDFGGEDNKTNIFASAALVKGQYAKSNFVGQEGNQGP
                     YKLVGKNGELYVLVISGSERVYVNGILLNRGENKDYTIDYNAGEILFTPLFTITSEMR
                     ITIEYQYSEQNYTRLVTYVGGTHENKKWSLGGYLYSESDLKNQPLQQSLSTEQTQILA
                     DAGDDMNLMVAPSAYLDSYSDNKILYKKTIINGVEVFEYSNNSQDVLYNVKFNQVAAN
                     KGNYILKNAAAISRIYEYIEPLNGIPQGNYEPIILLVAPIKTQVATFLGKYNPTEKTL
                     IDFEIGLSNNDKNLFSSIDDSNNTGLATKINARQRLFSKKWKVDAFANYQFAQKDFST
                     VERLYSIEFGRDWNLGTTTIGNQSYLVSGLQMTLPEKGNLTYQFEKLDFTGNFSGNRH
                     ILNANFKLKNWDILSRGSFLNSDATVSTSKFLRNQTQAKYHFKKNWIGTRLRLEDNQE
                     KNKTTNEFSALSQRFTEYGFFAGRGDSTKVFVELGYFKRA"
     gene            complement(2292..>3133)
                     /locus_tag="PLQ70_11245"
     CDS             complement(2292..>3133)
                     /locus_tag="PLQ70_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQE35236.1"
                     /translation="QWLDVRNFVKTNSNYRDAEVDWELTQKNIAKNVKKKMLNITQGF
                     LGSDENNFTTTLGREGSDYTAGIFAYCLNAESVTIWKDVPGVMNADPRYFENASLLSH
                     ISYREAIEMAFYGATVIHPKTLQPLQKKEIPLYVKSFINPMLPGTCVSKGSDSEWQIP
                     SFIVKRNQLLISLSSIDFSFIMEENISEIFALFHQFKLKVNLIQNSAISFSVCVEDKF
                     GNFKDLNPILSKKFKVDFNENVTLYTIRHFNDKAAQTVEQNKTVLLKQVSRETMQVVT
                     KES"
BASE COUNT          940 a          535 c          546 g         1112 t
ORIGIN      
        1 gctcttttaa aataacctaa ttccacaaaa accttggtgc tgtcgccacg tcctgcaaaa
       61 aaaccatatt cggtgaatcg ttggctcaaa gccgaaaact catttgtagt tttatttttt
      121 tcttgattgt cttccaatct caagcgggtt cctatccagt ttttcttgaa atgatatttg
      181 gcttgggttt gatttcgcaa gaattttgat gttgaaactg ttgcatcact attcaaaaaa
      241 ctacctcggc taagaatatc ccaatttttc aatttaaaat tagcatttaa aatatgacga
      301 ttgccagaga aatttccagt aaaatccaat ttttcgaatt gataagtcaa atttcctttt
      361 tcaggaagag tcatttgcaa acccgaaact agataacttt ggttgccaat tgtggtggtt
      421 cccaaattcc aatctcgccc aaattcaatg ctgtaaaggc gttcgactgt gctgaaatct
      481 ttttgtgcaa attgataatt ggcaaaagca tctaccttcc attttttaga aaacagccgc
      541 tggcgtgcat ttatttttgt tgccaaacct gtattattag aatcgtcaat ggacgaaaac
      601 aagtttttat cgttgttgct aaggccaatt tcaaaatcaa tcaaggtttt ttcggtggga
      661 ttgtattttc ctaaaaaggt ggcaacctga gtttttattg gtgccaccaa tagaataata
      721 ggttcgtaat ttccttgtgg gattccattt aggggttcaa tatattcata aattctacta
      781 atggcggcag cgtttttcag aatgtaattt cctttattgg cagccacttg attaaatttc
      841 acattgtaca aaacatcctg tgaattattc gaatattcga aaacttcgac cccattgatg
      901 atcgtttttt tgtacaaaat tttattatca gaataagagt ccagataggc agatggtgct
      961 accattaaat tcatatcatc acctgcatcg gccaaaattt gcgtttgttc agtcgaaaga
     1021 ctttgttgca gaggttgatt ttttaaatcg ctttcagaat acaaatagcc gcctaaactc
     1081 cattttttat tttcgtgagt tccgccaaca taagtgacta atcgggtgta attttgttca
     1141 gaatattggt attctattgt aattctcatt tcggaagtaa tggtaaatag cggtgtaaac
     1201 aggatttcac cagcattata atctatggtg taatctttgt tttcgccacg atttaaaaga
     1261 attccgttga cataaactcg ctctgaaccc gaaatgacaa gaacatacaa ttcgccattt
     1321 ttgcctacta atttgtatgg accttgattg ccttcttgtc ctacaaaatt acttttagca
     1381 tattgccctt tgaccaatgc cgccgaagca aaaatattgg ttttgttgtc ctcgcctcca
     1441 aagtcaaaac tggtcgaaat tccttgggct tttttattga aatttagaaa ttggcttttg
     1501 tggttttcaa gaaaaacatc accagcacga atattccatt tttcgctgaa aagttccatg
     1561 aaaatattgt caaattgatc tagcttttga gaatatccgc catcttgaag cggaatattg
     1621 ttgtcttgca aggaagctct cagacttact ttgtctgaaa gttttccggt gatttgcaaa
     1681 tcgagattgg aggttaaaac cgagttttgg ttgttgccaa tggtaatgcc tcgcgaaata
     1741 cttcctgatg tgttaaggcc ttcaaaaggg atgttttttt tctgcgaaat agtttctacg
     1801 gtatataaac ttcccttaga agcttcatta ctgaccattt tattggagtc ataaatccga
     1861 tattctttgg ttaaaatttc aggaagtttt aaatactgaa ccgttataga atctgatttt
     1921 gaagtcaatt tttcgtttaa taataatgta cctttttcga aatttatttt gtagaaacta
     1981 ctatcaatcg gttggttatt ggcgtcaagc aatttgaaaa aactggaatt tagactttct
     2041 ttttcaatgg gaatggtgtc gttcgaaaca agaatttttt tggttttgta tagcgaattg
     2101 ctttcctgag cgtggagttc agaaaagcct aatattgcca acaaaatcaa gatatttttt
     2161 aacataaata ctttaactcg aaagtgtcaa aagtagtatt tataattggg aaaaagtatt
     2221 cataaaaaaa ccgcaaattc tcaaatattt aaatttgaga atttgcggta aaattatttg
     2281 gaagaggttt gttaactttc cttagtaacc acttgcatcg tttcgcggct tacttgtttt
     2341 agcaaaacag ttttgttttg ttcgacagtt tgtgccgctt tatcattaaa atgtctgatg
     2401 gtgtaaagcg ttacattttc attaaaatcg actttgaatt tcttagaaag aattggattt
     2461 aaatctttaa aattcccgaa tttgtcttca acacaaaccg aaaaactgat ggctgaattt
     2521 tgaattaaat taactttcag tttaaattgg tgaaacagag caaatatttc gctaatgttt
     2581 tcttccataa taaaagagaa atctatagag gaaagcgaaa tcaaaagttg atttcttttg
     2641 acaataaaac tagggatttg ccattcagaa tcggatcctt tagatacgca agttccaggt
     2701 agcattgggt ttataaaaga ttttacatac aaaggaattt cctttttttg tagtggttgt
     2761 aacgtttttg ggtgaataac ggttgctcca taaaacgcca tttcaatagc ttcacgatat
     2821 gaaatatgac tcaacaaact cgcattttca aaatatcttg gatcggcatt cattactccc
     2881 ggaacgtctt tccagatggt tacactttcg gcattgaggc aataagcaaa aattcctgcg
     2941 gtgtaatctg agccttcacg acctagagtt gtggtgaaat tattttcgtc agaacccaaa
     3001 aatccttggg taatgtttag catttttttc tttacgtttt tggcaatatt tttttgagtt
     3061 aattcccaat ctacttctgc atctctataa tttgaattgg tttttacaaa attacgaacg
     3121 tccaaccatt ggg
//
LOCUS       DAOSNN010000279         3130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_31113_length_3130_cov_4.376585, whole genome shotgun sequence.
ACCESSION   DAOSNN010000279 DAOSNN010000000
VERSION     DAOSNN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31113_length_3130_cov_4.376585"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..403
                     /gene="rplL"
                     /locus_tag="PLQ70_11250"
     CDS             32..403
                     /gene="rplL"
                     /locus_tag="PLQ70_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008466917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQE35237.1"
                     /translation="MADLKQFAEQLVNLTVKEVNELATILKDEYGIEPAAAAVVVAAG
                     GGEAAAEEAQTEFTVVLKEAGASKLAVVKAVKELTGLGLKEAKDLVDSAPSNIKEGVS
                     KDEAEGLKKSLEEAGAVVELK"
     gene            661..>3130
                     /gene="rpoB"
                     /locus_tag="PLQ70_11255"
     CDS             661..>3130
                     /gene="rpoB"
                     /locus_tag="PLQ70_11255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQE35238.1"
                     /translation="MITNQTERLNFASTKNIPQYPDFLDVQVKSFKDFFQLETKSDER
                     GDEGLYNTFMENFPITDTRNNFVLEFLDYFVDPPRYTIQECIERGLTYSVPLKARLKL
                     YCTDPEHEDFETIVQDVYLGTIPYMTPSGTFVINGAERVVVSQLHRSPGVFFGQSFHA
                     NGTKLYSARVIPFKGSWIEFSTDINSVMYAYIDRKKKLPVTTLFRAIGFERDKDILEI
                     FDLAEEIKVSKTGLKKYIGRKLAARVLNTWHEDFVDEDTGEVVSIERNEIILDRDTII
                     DKDNVEEIIDSNVKSILLHKEDSNAVDYSIIHNTLQKDPTNSEKEAVEHIYRQLRNAE
                     PPDEETARGIIDKLFFSDQRYNLGEVGRYRINKKLGLDTPMEKQVLTKEDIITIVKYL
                     IELINAKADIDDIDHLSNRRVRTVGEQLSQQFGVGLARMARTIRERMNVRDNEVFTPI
                     DLINAKTLSSVINSFFGTNQLSQFMDQTNPLAEITHKRRLSALGPGGLSRERAGFEVR
                     DVHYTHYGRLCPIETPEGPNIGLISSLGVYAKVNGMGFIETPYRKVTKGVVDLESTPV
                     YLSAEEEEGMMISQANIEMDATGKILADNVIARQEGDFPVIDPSKVDYADVAPNQIAS
                     ISASLIPFLEHDDANRALMGSNMMRQAVPLLRPEAPIVGTGLERQVASDSRVLINAEG
                     PGTVEYVDANIITIKYDRSEEERMVSFEPDEKTYNLIKFRKTNQSTSINLKPIVRKGD
                     RVVLGQVLSEGYATQNGELALGRNLKVAFMPWKGYNFEDAIVISEKVVRDDIFTSIHV
                     DDYSLEVRDTKLGNEELTNDIPNVS"
BASE COUNT         1066 a          515 c          632 g          917 t
ORIGIN      
        1 ttttacaaat cattttaaaa cgatagaaaa aatggcagat ttgaaacaat tcgcagaaca
       61 attagttaac ctaacagtaa aagaagttaa cgaattagca acaatattaa aagatgagta
      121 cggaatcgaa ccagctgctg cagctgtagt agttgctgct ggtggtggag aagctgctgc
      181 agaagaagct caaactgaat ttacagttgt attgaaagaa gctggtgctt ctaaattggc
      241 agttgtaaaa gcagttaaag aattaactgg tttaggtttg aaagaagcta aagatttagt
      301 ggattcagca ccatcaaaca ttaaagaagg tgtttctaaa gacgaggctg aaggtcttaa
      361 aaaatcttta gaagaagctg gagcagttgt tgaattaaaa taattcactc cgctttagag
      421 aattaggttt aggtcttgga tacgaatcca atggcctaaa ccatttttcg tataataaat
      481 ttacactcta aaatttatct taaagagcgt aaaataaata aaacttttga tcaatacgaa
      541 gaaagaaatg agcgaacttg gttcattttt aagaggtgtt gatcgtaaaa agaaagtaga
      601 gattacaccg tgtttttgca caaaaaaaat actttttttt aatcaaaatt ttgtccattg
      661 atgataacaa atcagactga aagattgaat tttgcctcca caaaaaacat acctcaatat
      721 ccagattttc tagatgttca ggtaaaatcg tttaaagatt tcttccaatt ggaaactaaa
      781 tctgacgaga gaggcgacga aggtttatac aacaccttca tggaaaactt tccaattact
      841 gatacaagaa ataactttgt attagaattc ctcgactatt ttgtagatcc accacgttac
      901 accattcaag aatgtattga aagaggactt acgtatagtg tgcctttaaa agctaggtta
      961 aaactatact gtacagaccc agagcacgaa gattttgaaa ctattgttca ggatgtttat
     1021 ctaggtacaa ttccttatat gactccttcg ggtacttttg taatcaatgg tgccgagcgt
     1081 gttgttgtat cgcaattgca ccgttctcca ggggttttct ttggacaatc attccatgcc
     1141 aatggaacaa aattatattc tgccagagtt attcctttca aaggatcttg gatagaattt
     1201 tcaaccgata tcaacagcgt aatgtacgcc tatatcgaca gaaagaaaaa attacctgta
     1261 acgactttat tccgtgccat cggtttcgaa agagacaagg atatcttgga aattttcgac
     1321 ctagccgaag aaattaaagt gtctaaaaca ggacttaaaa aatatatcgg tagaaaatta
     1381 gccgctcgtg tattgaatac ttggcacgaa gatttcgtgg atgaagatac cggcgaagtt
     1441 gtttctattg aacgtaacga gattatcctt gatcgtgaca ccattatcga caaagataat
     1501 gtggaagaaa tcatcgattc taacgttaaa tctattttgt tgcacaaaga agatagcaat
     1561 gcggttgact attctatcat tcacaacacg ctacaaaaag atccaaccaa ctctgaaaaa
     1621 gaagccgttg agcacatcta cagacaattg cgtaacgctg aaccgcctga tgaagaaact
     1681 gctcgtggta ttatagataa attattcttc tctgaccaac gttacaattt aggtgaagta
     1741 ggtcgttata gaattaacaa aaaactgggt cttgatactc caatggaaaa gcaagtgctt
     1801 accaaagaag atatcatcac cattgtgaag tatttgattg agttaatcaa tgcgaaagct
     1861 gatattgatg atatcgatca cttgtcaaac cgtcgtgtta gaacagttgg agagcaattg
     1921 tctcaacaat ttggtgttgg tttggctcgt atggcaagaa ccattcgtga gagaatgaac
     1981 gttagagata acgaggtgtt tacaccaatc gatttgatta atgctaaaac attgtcgtcg
     2041 gttatcaact ctttctttgg aaccaaccag ttgtctcaat ttatggatca aacgaatcca
     2101 ttggctgaga ttacgcacaa aagaagatta tctgcacttg gaccaggtgg actttcgaga
     2161 gaaagagctg gatttgaggt tcgtgacgtt cactatacgc actacggacg tttatgtcca
     2221 attgaaactc ctgagggacc aaacattggt ttgatttcat ctttgggtgt ttatgctaaa
     2281 gttaacggaa tgggtttcat tgaaacacct taccgtaaag taactaaagg tgtggtagat
     2341 ttagaatcta ctccggttta cttgagcgca gaagaagaag aaggaatgat gatttctcag
     2401 gcaaacattg aaatggatgc tacaggaaaa atccttgccg ataacgtaat tgctcgtcaa
     2461 gaaggggatt ttccagttat tgacccgtca aaagtggatt atgctgacgt tgctccaaac
     2521 caaattgctt cgatttcagc ttcattgatt cctttcttgg aacacgatga tgcgaataga
     2581 gccttgatgg gatctaacat gatgcgtcaa gccgtaccat tgttgcgtcc agaagctcct
     2641 attgttggaa caggtttaga gcgtcaagtt gcttcagatt ctagagtatt aattaatgct
     2701 gaaggtcctg gaactgttga atacgtagat gcaaatatca tcaccatcaa atacgatcgt
     2761 tccgaagaag aaagaatggt aagttttgag ccagatgaaa aaacatataa tctaattaaa
     2821 tttagaaaaa ccaatcaaag tacaagtatt aacttgaaac caatcgtaag aaaaggagac
     2881 agagtggttc ttggacaagt attatcagaa ggatatgcta ctcaaaatgg tgaattagct
     2941 ttaggtcgta acctaaaagt agcgttcatg ccttggaaag gatataactt tgaggatgcg
     3001 attgtgattt ctgaaaaagt agttcgtgac gatattttta cctctattca cgttgatgat
     3061 tattcattag aagtgagaga tactaaatta ggtaacgaag aattaacaaa tgatattcct
     3121 aacgtttctg
//
LOCUS       DAOSNN010000280         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_31491_length_3101_cov_4.478989, whole genome shotgun sequence.
ACCESSION   DAOSNN010000280 DAOSNN010000000
VERSION     DAOSNN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31491_length_3101_cov_4.478989"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLQ70_11260"
     CDS             complement(<1..669)
                     /locus_tag="PLQ70_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQE35239.1"
                     /translation="MDLNFNKNEDHNKLLLTDLRQKLAKVKLGGGEKRIQKLHSEGKM
                     TARERIDYLLDAKGKSIEIGAFVGEGMYAEHGGCPSGGVIVKIGYIKGKQCIVVANDA
                     TVKAGAWFPITAKKNLRAQEIAMENRLPIIYLVDSAGVYLPLQDEIFPDKEHFGRIFR
                     NNALMSSMGITQISAVMGSCVAGGAYLPIMSDEALIVDKTASIFLAGSYLVKAAIGEN
                     IDNET"
     gene            complement(830..1825)
                     /gene="lpxD"
                     /locus_tag="PLQ70_11265"
     CDS             complement(830..1825)
                     /gene="lpxD"
                     /locus_tag="PLQ70_11265"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQE35240.1"
                     /translation="MTYTIQEINEVLKGTIVGDTSIKITAPEQLELASNSEISFIGNK
                     KYEKCWQNSKACVAVVNEDISINPGENRAFIKVKNADLAMSQVLSLFALPTPLFNTDI
                     HPSAIIDITAIIGKGTRIGAGSYIGPKVVLGENVTIYPNVTILDECTVGKNTVIWPGA
                     VIRERCHLGDHCIIHPNATIGADGFGFRPCPEKGLVKIPQIGNVIIGNSVEIGANSCV
                     DRGKFSSTVLGDGCKIDNLVQIGHNSILGKFCIMAGNSGLAGSVTLGNGVIIGGSASI
                     KDHTTIGDGAIVGAGSGVTCDIPPGKTMLGYPAVEARDALKQWAILKRMVSDSKK"
     gene            2020..2184
                     /locus_tag="PLQ70_11270"
     CDS             2020..2184
                     /locus_tag="PLQ70_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35241.1"
                     /translation="MASFSSGCVAIQEEYMKIMVSWLDPKQNPTIIMGNLDVLKAGFK
                     KKDLTSYKNC"
     gene            complement(2214..>3101)
                     /gene="glgP"
                     /locus_tag="PLQ70_11275"
     CDS             complement(2214..>3101)
                     /gene="glgP"
                     /locus_tag="PLQ70_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQE35242.1"
                     /translation="VGLRLSHIANGVSKLHGEVSRDMWKEYPGICPIIHITNAQNKKY
                     WADAWLDEAFENKDREALIKRKTRLKAKLFDVVADQTGKLFDPNILTIVWARRFADYK
                     RPDLITRDYDRFIKLLSNTKRPVQIIFAGKPYPMDYGAIHNFDNLTHISKNIKNMAVL
                     TGHELKLSRQLKEGADVWLNNPRVTREASGTSGMTAAMNGAVNFSTNDGWVREFKDLN
                     KTQNSFVLPIVDHNLTTHQQDEMDLDNLLEMLENKILPLYYDTPKKWWNLVETSMKQI
                     VPFFDSSRMVDEYYKKLYK"
BASE COUNT          925 a          616 c          523 g         1037 t
ORIGIN      
        1 agtttcgttg tcaatatttt cgccaatggc tgctttaacc aaataacttc ctgcaaggaa
       61 aatactagct gttttgtcca caatcaaagc ttcgtcactc attatcggaa gataagcacc
      121 accagcaaca caacttccca ttacggccga aatttgggta attcccatac tgctcatcaa
      181 ggcattattt ctaaaaatgc gaccaaaatg ttctttgtca ggaaaaattt cgtcttgcaa
      241 aggcaaataa accccagccg aatccactaa ataaataata ggcaatctgt tttccattgc
      301 aatttcttgc gctctgaggt ttttctttgc cgtaatggga aaccaagcac ccgcttttac
      361 ggtagcatca ttggctacaa caatacattg tttccctttg atatacccaa ttttcacaat
      421 cacaccaccc gacggacaac caccgtgttc ggcatacatt ccttctccaa caaaagcacc
      481 aatttcaatg ctttttcctt tagcatctag caaataatca atgcgttctc gtgcggtcat
      541 tttgccttcg gaatgtagtt tttggatgcg tttttctcca ccgcccagtt ttactttggc
      601 caatttttga cgcaagtcag taagaagtag tttgttgtgg tcttcgtttt tattgaagtt
      661 caaatccata atgcaattgt gtttgtctaa aaatagcgat ggctaaatta cgaaaactgt
      721 tggaacaaat tttagaatat tcgagagaaa tattttacct cagattcgca gatttttttt
      781 taaataattt taaaatttgc gaatttgcgg tcaattttat ggtaacaatt tatttcttcg
      841 aatcgctgac catacgtttt agaatcgccc attgttttaa ggcatcacga gcttcgacag
      901 caggataacc caacattgtt tttccgggtg gaatatcaca agtaacacca gaaccagctc
      961 caacgatagc gccatcacca atcgtggtgt gatcttttat ggaggcgctt ccgccaatga
     1021 taacaccatt tcctaaagtt acagaacccg ctaatcctga atttccagcc attatgcaaa
     1081 atttacctaa tatgctattg tgaccaattt gaactaaatt gtcaatcttg cagccatcgc
     1141 ctaaaacagt agagctaaat tttcctctat caacacacga attagctcca atttcgacac
     1201 tatttcctat aattacgttt ccaatttgtg gaatttttac caagcctttt tctgggcaag
     1261 gtctaaatcc aaaaccatct gcaccaatag ttgcattggg atgtataatg caatgatctc
     1321 ctaaatgaca acgctcccga ataactgctc ctggccagat tactgtgttt ttgccgactg
     1381 tacattcgtc cagaatagtt acattggggt aaatagtaac attttcgcca agaactactt
     1441 taggaccaat ataacttcca gctccaattc gagttccttt tcctataata gccgtaatat
     1501 caataattgc tgatgggtga atatctgtgt tgaatagtgg agtaggtagg gcaaaaagag
     1561 ataaaacttg tgacatagcc aaatcagcat ttttaacttt tataaatgct ctgttttcgc
     1621 caggatttat ggaaatatct tcgttaacta cagcaacaca cgccttagaa ttttgccaac
     1681 atttttcgta ttttttgttt ccaataaaag aaatttccga attactggct aattctaatt
     1741 gttctggtgc ggttattttt atggaagtat cgccaacaat tgtgcctttt aaaacttcgt
     1801 ttatttcttg aattgtatat gtcattaagg ggtcctgatt tagatggatt tgtatttacc
     1861 aaataaaacg aatttgaaca ggaattccta attaattatg cataactaaa ctaattaggt
     1921 cttttaatag gttttattta taaaatgata taataatcat acaatttttt aaaattggta
     1981 ttgcagccga agtcaacatt tatttcaaat gacataaaaa tggcttcttt cagttcaggt
     2041 tgtgtggcta ttcaagaaga atatatgaag attatggtta gttggctcga ccccaaacaa
     2101 aatccaacta ttattatggg aaatttggat gttttgaaag cgggatttaa aaaaaaagac
     2161 ctaacaagtt ataaaaactg ttaggtcagc attattctta ctgaaacagt aatttactta
     2221 tacaacttct tgtagtattc atcgaccatc cttgatgaat caaaaaacgg aacaatttgt
     2281 ttcatactgg tctctactaa attccaccat ttttttggag tatcataata taaaggaagt
     2341 attttgtttt caagcatttc aagtagattg tccaaatcca tttcgtcttg ttggtgcgtg
     2401 gtcaggttat gatctacgat tggcaatacg aaagaatttt gggttttgtt caaatcttta
     2461 aattcacgaa cccaaccatc atttgtcgaa aagttgacag cgccgttcat agccgccgtc
     2521 attccagatg ttcccgaagc ttctctggtg actctcgggt tgttcaacca aacatcggca
     2581 ccttctttta gctgacgcga taatttcaat tcgtgtcccg taagaaccgc catatttttg
     2641 atgtttttac tgatatgggt tagattgtca aagttgtgaa tggctccata atccattgga
     2701 tacggttttc ctgcaaaaat gatttgaacc ggtctcttgg tattggataa caacttgata
     2761 aaacggtcgt aatctctggt aattaaatcg ggacgtttgt aatcggcaaa tcgtcttgcc
     2821 caaacaatgg ttaaaatatt gggatcaaac aattttccgg tttggtcagc gacaacatca
     2881 aaaagctttg cttttaatct tgtttttctt ttgattaacg cttctctgtc cttgttttca
     2941 aaggcttcat ctaaccaagc atcagcccaa tatttcttgt tttgagcatt ggtaatatga
     3001 ataattggac aaattccggg atactctttc cacatatctc gggaaacttc tccgtgcaat
     3061 tttgaaactc cgttggcaat atgactcaat cgaagtccaa c
//
LOCUS       DAOSNN010000281         3047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_32205_length_3047_cov_4.325535, whole genome shotgun sequence.
ACCESSION   DAOSNN010000281 DAOSNN010000000
VERSION     DAOSNN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32205_length_3047_cov_4.325535"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="PLQ70_11280"
     CDS             <1..105
                     /locus_tag="PLQ70_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQE35243.1"
                     /translation="DDTAEAKKRIMRECGIHVVDSPAEIGKKVKEVLG"
     gene            184..612
                     /locus_tag="PLQ70_11285"
     CDS             184..612
                     /locus_tag="PLQ70_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35244.1"
                     /translation="MYKELKKIKVQNQFTFSTDDSLEEVCNAPEAGSGIFLVYAVDGE
                     EKVLIMVGSTGTIQNDGTLKIKNGGLFDKIVNGHQFAKTGRKYSWPAQMKLENISLLE
                     VAWYETFNDKNKVIPTFLEGQILQNFLDENAKLPRWNVAF"
     gene            complement(696..1025)
                     /locus_tag="PLQ70_11290"
     CDS             complement(696..1025)
                     /locus_tag="PLQ70_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HQE35245.1"
                     /translation="MREHLAHFDIAGFTYYEGVLAFNELKIGVQLQLKLEEENKYDAR
                     AVAIYYNEFKLGFVPRSENRIIYKLLKVGIDCLEVRIQRVDKKENPENQIGVVIHLVI
                     TEKATQE"
     gene            1120..2127
                     /locus_tag="PLQ70_11295"
     CDS             1120..2127
                     /locus_tag="PLQ70_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HQE35246.1"
                     /translation="MSITKNAIIRYRALDRCFSNFGRKFSNEDLLKACNDALYDYNFT
                     NEGIKRRQLFEDIKFMESPQGWEIPLQRNKEGRKVFYRYETKDFSINNQPINDFEVEK
                     LKSALLVLSRFKGMPQFEWVNELILKLQQAFHLDSSKKEIISFDANEYLKNIHLIGDL
                     FNAIIYQKVLELKYQKFDNEQSKTFEFHPYYLKQYNNRWFIIGRFPKYENLTHFALDR
                     IVQFKEIDTKFIGTTINFNEYFEDVIGISKPKNSTIEKVVLLATPTLKPYITTKPIHG
                     SQKRISENENGYLFSIDVFINFELENLLLSYGETLKIVEPKSLQETIKNRLLKNLENY
                     L"
     gene            2176..>3047
                     /locus_tag="PLQ70_11300"
     CDS             2176..>3047
                     /locus_tag="PLQ70_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HQE35247.1"
                     /translation="MPLFQNSVVTKQLQSQDKSKLLEGWNNFKNHFHNPTIQDNIRSS
                     KEIQYQGEFLIDLFVNVLGYTKNPTPNFNLNTEYNNVKDSKAADGAILIKDKVRAVIE
                     LKGTNTTDLNKIEVQAFGYKNNQPDCVYVITSNFEKLRFYIDNATEYLEFNLFTLTEK
                     EFELLYLCLAYENIANDIPKKLKEQSLSQEKDITKTLYTDYSLFKRELHHDLVKQNPD
                     FDALELFQKSQKLLDRFLFIFFAEDRNLLPTNLIFRINQEWRQLQEMRINISLYDRYK
                     IYFNDLNQGAKVVLP"
BASE COUNT         1115 a          475 c          468 g          989 t
ORIGIN      
        1 gacgatacag ccgaagccaa aaaacgcatt atgagagaat gtggaattca cgtggttgac
       61 tctcctgctg aaattggaaa aaaagtaaaa gaagtattgg gataaataaa ttccaatgtt
      121 ttaaataaca aattccaaaa tgcctcacta atttggtgag gcattttaat taataaataa
      181 gaaatgtaca aagaattaaa aaaaatcaaa gttcaaaatc agtttacttt ttcaactgat
      241 gatagtttag aagaagtttg taatgcccca gaagccggaa gcggaatttt cttggtatat
      301 gcagttgatg gagaagaaaa agtattgatt atggtaggtt ctacaggaac catccaaaac
      361 gacggaactt tgaaaatcaa aaatggtgga ttgttcgaca aaatcgtcaa cggtcaccaa
      421 tttgccaaaa caggaagaaa atattcttgg ccagcccaaa tgaaattaga aaatatctca
      481 ctattggaag tggcttggta cgaaactttc aacgacaaga acaaagtaat tccaactttc
      541 ttagaaggac aaattttgca aaatttcttg gacgaaaatg ccaagttgcc aagatggaac
      601 gtggcttttt aaatccaaaa tccttaaaaa aacgaaaatg cccgcgcaat tcgtagggca
      661 ttttttgttt aaaggattca acatacaaaa attatttact cttgggttgc tttttctgtt
      721 atgactaaat gaattacaac tcctatttga ttttcgggat tttctttttt atctactcgt
      781 tgtattcgaa cttcaaggca atcaattcct acttttagta atttgtaaat aattctgttt
      841 tcacttctag gaacaaatcc taatttgaat tcgttgtaat aaatagcaac tgctcttgcg
      901 tcatatttat tttcttcttc taattttaat tgcaattgga cacctatttt taactcatta
      961 aaagctaaaa caccttcgta ataggtaaat cctgcaatat caaaatgggc taaatgttct
     1021 ctcataactt ttgtttttaa atcttcaaca aaactatttt gtattagtgc agtacaaatg
     1081 catttatttg ttttattttt gattcataaa aaaatcagta tgtcaatcac taaaaacgca
     1141 ataattcgtt atcgagcttt agaccgatgt tttagtaatt ttggtcgtaa attttcaaac
     1201 gaagatttat taaaagcctg taatgatgca ctctatgatt ataattttac taatgaggga
     1261 attaagcgaa gacaactttt tgaggacatt aagtttatgg aaagtccaca aggttgggaa
     1321 attccgttgc aacgaaataa agaaggtaga aaagtttttt acaggtacga aaccaaagat
     1381 ttttctataa acaaccaacc cataaacgat tttgaagtag aaaaactaaa atccgctttg
     1441 ctagttttat ctcgtttcaa agggatgcca caatttgaat gggttaacga actcattttg
     1501 aaattacaac aggcgtttca tcttgacagt tcaaaaaaag aaatcatcag ttttgatgct
     1561 aacgaatatt taaaaaatat tcatcttatt ggcgatttat tcaatgccat tatctatcaa
     1621 aaagtattag aattgaaata tcaaaagttt gataacgaac aaagtaaaac tttcgagttt
     1681 catccttatt atctcaaaca atacaacaac cgttggttta ttattggtcg atttccgaaa
     1741 tatgaaaacc tcactcattt tgcattggat agaattgttc aattcaaaga aatagacaca
     1801 aaatttattg gaaccacaat aaattttaat gaatattttg aagatgttat tggtatttct
     1861 aaacctaaaa attcaacaat tgaaaaagta gtattgctgg caacacctac tttaaagcct
     1921 tacattacaa ctaaacccat tcacggttca caaaaaagaa tttctgaaaa tgaaaatggg
     1981 tatctatttt ctatcgatgt ttttatcaat ttcgaattag aaaatttatt gctttcgtat
     2041 ggcgaaacgc taaaaatagt agaaccaaaa tcgttgcaag aaactattaa aaatcgtttg
     2101 ctaaaaaatt tagaaaacta tctttaattt ttttatttac ttaacttgta ccagatatta
     2161 aaacacaact attctatgcc tttatttcaa aattcagttg ttaccaaaca gttacaaagc
     2221 caagataaat ctaaactact tgaagggtgg aataatttta aaaaccattt tcacaatcca
     2281 accattcaag acaatatacg ttcgagcaaa gaaattcaat accaaggtga atttttgatt
     2341 gatttgtttg taaatgtttt ggggtacacc aagaatccaa caccaaattt taatttaaat
     2401 actgaataca ataacgtaaa agatagtaaa gctgctgatg gtgctatcct cataaaggat
     2461 aaagttaggg cagtaattga gctgaaagga accaatacta ccgatttgaa caaaatcgaa
     2521 gttcaggctt ttgggtacaa aaacaatcag cctgattgcg tttatgtaat cacttctaat
     2581 tttgaaaaac tacgttttta tattgataat gctaccgaat atctagagtt taaccttttt
     2641 acgcttaccg aaaaggaatt tgaattgctc tatttgtgtt tggcttatga gaacattgcc
     2701 aacgatattc ccaagaaact caaagaacaa tccttgagtc aggaaaaaga tattaccaaa
     2761 actttatata ccgattattc ccttttcaaa cgggagttac atcatgattt agtaaaacaa
     2821 aatcctgatt ttgatgcctt ggaattgttc caaaaatcgc aaaaattatt agatcgtttt
     2881 ctatttattt tctttgcaga agatagaaat ttattgccaa cgaatttgat tttcagaatc
     2941 aatcaggaat ggcgacaatt gcaggaaatg cgaataaata tttcgttgta tgaccgttac
     3001 aaaatctatt ttaacgattt gaaccaaggt gccaaagtcg ttctgcc
//
LOCUS       DAOSNN010000282         3013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_32690_length_3013_cov_4.916498, whole genome shotgun sequence.
ACCESSION   DAOSNN010000282 DAOSNN010000000
VERSION     DAOSNN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32690_length_3013_cov_4.916498"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PLQ70_11305"
     CDS             complement(<1..162)
                     /locus_tag="PLQ70_11305"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35248.1"
                     /translation="MFLFGISTQLFAQRTVEKLNRGLVAMRLNSSQVYVGWRMLGTEP
                     TDVSYNLYCN"
     gene            complement(398..1588)
                     /locus_tag="PLQ70_11310"
     CDS             complement(398..1588)
                     /locus_tag="PLQ70_11310"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQE35249.1"
                     /translation="MNNLLSDRINNLATSQTLAMAALARELKAQGKDIISLSLGEPDF
                     NTPDFIKEAAKKAIDENYSTYSPVDGYGELKEAICRKFKRDNGLDYKPSQIVVSTGAK
                     QSLYNIAQVMLNDGDEVILPAPYWVSYFEIVKLSGGVPVEVPTSVETDFKITPEQLEA
                     AITPKTKMMWFSSPCNPSGSVYNREELTALAKVLEKHPNIIVVADEIYEHINFSGTFC
                     SIGSIPGMLEKTVTVNGVAKAFAMTGYRIGYIGAPEFIAKACTKIQGQVTSGANSVAQ
                     RATITAVDADPSVLNHMVQAFHSRRDLVVGLLKEIPGIKINVPEGAFYVFPDVSSFFG
                     KTLKGTEIKDANDMAMYLLAEACVATVTGDAFGNPNCIRFSYATSEEQLTEALKRIKE
                     ALAA"
     gene            complement(1637..2731)
                     /locus_tag="PLQ70_11315"
     CDS             complement(1637..2731)
                     /locus_tag="PLQ70_11315"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQE35250.1"
                     /translation="MNNVAPTFAKQDNLKFFRTLNTRVNSYFKENNIAKTGNWKIHLK
                     TIILFTVFLVPYFLILTLPMPFWGHLLLTIVMGIGMAGIGMNVMHDANHGSYSTKSWV
                     NKFMGGTIYVLAGNVHNWQVQHNVLHHTYTNIIGHDEDLEAGRIMRFSKEAEWHKFHR
                     FQQYYALFLYGLLTFNWAITTDFKQMRSYLKRKLSYGEPKSPKILWTTLIITKLIYIA
                     IWIVLPMIIGITWWKVLVGFFVMHYTAGLILSIVFQLAHVVDEAANPVPNEEGEMENT
                     WAIHQLFTTVNFAPKNKIVNWYTGGLNHQIEHHIFPHISHVHYNKISKIVKETAKECQ
                     LPYHEYKTFRAAVVAHFKHLKELGMKPALQ"
     gene            2838..>3013
                     /locus_tag="PLQ70_11320"
     CDS             2838..>3013
                     /locus_tag="PLQ70_11320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQE35251.1"
                     /translation="MDEIQKYFPYLTDIQKEQFEKLDFLYHDWNAKINVISRKDIDEL
                     YTKHILHSLGIAKVV"
BASE COUNT          900 a          536 c          548 g         1029 t
ORIGIN      
        1 gttgcaatac aggttatagc tcacatctgt tggttcggtt cccaacattc tccagccgac
       61 atagacttga ctgctgttta aacgcattgc aaccaaacct cgattgagtt tttcaacagt
      121 gcgttgagca aaaagttggg tggatattcc aaataggaac attagaaaaa agatgatttt
      181 tcgaaatgag ttttgagtaa tttttttatg cataattatg gttaatagtt gttggttagt
      241 ttgttgttaa cacataaaat agattgtagc cgctaaagtt aagcttaaaa aaaagttata
      301 ctatcctgta ctatcctacc catgaatttt ttgagttaat caaaaaaaag cctcaagaga
      361 aatcttgagg cttgtactaa tataaaaaga ctttcgacta tgctgccaat gcttctttga
      421 ttctttttaa tgcttctgtt agttgctctt cgcttgtagc ataagagaaa cggatgcagt
      481 ttggatttcc aaaagcatct ccagttactg tagctacaca agcttcagcc aaaagataca
      541 ttgccatatc atttgcatct ttaatttcag tgcctttcaa agttttacca aagaaagaag
      601 aaacgtctgg gaatacatag aaagctcctt caggaacgtt gattttgatt cctggaattt
      661 ctttcaacaa tcctaccact aaatctctac ggctgtggaa ggcttgaacc atgtgattca
      721 atacgctagg gtcagcatca acagcggtga ttgtagcacg ttgtgccaca gaatttgctc
      781 ctgaagttac ttgtccttgg atttttgtac atgcttttgc gataaattct ggtgctccaa
      841 tgtaaccaat tctgtatcca gtcattgcga aagctttggc aactccgttt acagtaactg
      901 ttttttctaa cattccaggg attgatccaa tactgcaaaa agttccagag aaattgatgt
      961 gttcgtagat ttcgtcagca accacgataa tgtttgggtg tttttccaaa actttagcta
     1021 aagcagtcaa ttcttctctg ttgtaaaccg aacctgatgg attacaaggc gaagagaacc
     1081 acatcatttt tgtttttggc gtgatggcag cttctaattg ctcaggagtg attttgaaat
     1141 cagtctctac agaagttgga acttctacag gaactccgcc tgataatttt acgatttcga
     1201 agtaagaaac ccagtaaggt gcaggcaaga ttacttcgtc accgtcgttt agcattactt
     1261 gcgcaatgtt gtataaagat tgttttgctc ctgttgaaac cacaatttgt gatggtttgt
     1321 aatccaatcc gttgtctctt ttgaattttc tgcaaatagc ttctttcaat tcgccatatc
     1381 catcaactgg agagtaagtg ctgtagtttt cgtcgatagc ttttttggca gcttctttaa
     1441 tgaaatcagg agtgttaaaa tctggttcac ccaaactcaa actgatgatg tcttttcctt
     1501 gtgcttttaa ttctctggcc aaagcagcca ttgctaatgt ttgtgatgta gctaagttgt
     1561 taattctgtc tgaaagcaaa ttgttcattg aaatgtaatt ttgatttata tttttatatt
     1621 atggttttgt tcgtttttac tgtaaagcgg gtttcattcc cagttctttt aagtgtttaa
     1681 aatgtgccac aactgcagct ctaaaagttt tatattcgtg atagggcaat tgacattctt
     1741 ttgccgtttc ttttacaatt tttgaaattt tattataatg tacatggctg atgtgcggaa
     1801 aaatatggtg ctcaatttga tggttcaaac ctccagtgta ccagtttaca attttgtttt
     1861 ttggcgcaaa atttactgtg gtaaacaatt gatgaatggc ccaagtgttt tccatttcac
     1921 cttcctcgtt tggaactgga ttagcagctt cgtcaactac gtgtgctaat tggaacacga
     1981 tgctcaagat taatccagcc gtgtaatgca tcacgaaaaa tccaaccaaa actttccacc
     2041 aagtgattcc gataatcatt ggcaaaacaa tccaaatcgc tatatagata agtttggtaa
     2101 taattaaagt cgtccaaaga attttagggc tttttggttc gccataagat agttttcttt
     2161 tcaggtaact tctcatttgc ttgaaatcgg tagtgatggc ccaattgaaa gtcaataaac
     2221 catataaaaa taaggcataa tattgttgaa atctgtggaa tttatgccac tcagcttctt
     2281 tggaaaaacg cataattcga cctgcctcta aatcttcgtc gtgaccaata atgttagtgt
     2341 aagtatgatg taaaacattg tgttgcactt gccagttatg aacatttcca gccaaaacat
     2401 aaattgttcc tcccataaat ttattcaccc aacttttggt cgaataggaa ccgtgattgg
     2461 cgtcgtgcat cacattcatt ccgattcctg ccattccaat tcccataact atggtcaaga
     2521 gtaaatgtcc ccaaaaaggc ataggcaaag tgagtatcaa aaaatacggt accaagaaaa
     2581 cagtaaaaag aattatcgtt tttaaatgga ttttccagtt gccagttttg gcaatattgt
     2641 tttctttgaa gtagctgttc acccgagtgt taagcgttct gaagaactta agattgtctt
     2701 gctttgcaaa tgtaggggca acattgttca ttgttatttt tgaatattgc gcaaagataa
     2761 ttattataaa ttttcaatct gcaaaattga gttaaaattc aaaactaaaa tctgtacttt
     2821 tgttaaaatt tataacaatg gatgagattc aaaagtattt tccttattta actgatattc
     2881 agaaagaaca atttgagaaa ctggattttt tataccacga ttggaatgcc aaaatcaatg
     2941 tcatttcccg aaaagatatt gatgaattat acaccaaaca tattttgcat tccttgggga
     3001 ttgcgaaagt cgt
//
LOCUS       DAOSNN010000283         2930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_33848_length_2930_cov_4.621217, whole genome shotgun sequence.
ACCESSION   DAOSNN010000283 DAOSNN010000000
VERSION     DAOSNN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33848_length_2930_cov_4.621217"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLQ70_11325"
     CDS             complement(<1..597)
                     /locus_tag="PLQ70_11325"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HQE35252.1"
                     /translation="MNKITQLFNIKYPIIQAGMIWNSGYKLASAVSNAGGLGLIGAGS
                     MYPEVLREHIQKCKKATDKPFGVNVPMLYPNIEEIMTILVEEGVKIVFTSAGNPKTWT
                     PFLKEKGITVVHVVSSTFFALKAQDAGVDAVVAEGFEAGGHNGREETTTFTLIPMVKE
                     KIKIPLIAAGGIANGRGMLAAMVLGAEGVQVGSRFAAST"
     gene            complement(598..2226)
                     /locus_tag="PLQ70_11330"
     CDS             complement(598..2226)
                     /locus_tag="PLQ70_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQE35253.1"
                     /translation="MKKIILFLFLVSSFVGFSQEDAWVYFNDKPDSATYLSNPLTMLS
                     QRALDRRSNQGIALDSKDVPIYQPYIDQVATSTGISVKAKSKWFNCVHVRGSQADINA
                     LKLLSFVLNVDFADKTLNSSPNKTKAKLKYTPVNKTMETAVIYNYGNSLNQVQMLNGH
                     LLHQANYTGSGKIIAVLDSGFPGVNTATPFQRLRDNTQILGGYNYVTKSSDFYSGGTH
                     GTAVLSLMGGFVDGQLVGTAPDAKYYLYITEDDASENPVEESNWVEAAEEADRVGVDI
                     ITTSLGYFEFDDASYGHTYADMTGNAAFASKGANVAFSKGIVVLASAGNEGANPEPHI
                     GVPAEALNVIAVGAVKDDRTYASFSSIGPSYDGRVKPDVMAQGQYPYTYSGGTINNGG
                     SGTSYSCPILAGMVACLWQALPGKNAQQIKQLVVQSSDNFAEPAVKSRTQYGYGIPNF
                     SLALSNGLSVAAFSTNDFKVYPNPTDDSISVTLPNGSEVRTIAIYTILGQKVLEKNIS
                     TQSPTISLKSLNSGIYFYKIESNGISESGKIIKQ"
     gene            complement(2254..>2930)
                     /locus_tag="PLQ70_11335"
     CDS             complement(2254..>2930)
                     /locus_tag="PLQ70_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006265447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQE35254.1"
                     /translation="GELTKPEVREIALELDLVTADKKDSQGLCFIGKVRLPEFLQQKL
                     QPKDGIIYQIDKDSPIYNLEKPAHLSVEEALAFDAKKIDYTPEMGKVIGKHQGAHYFT
                     IGQRKGLNVGGTTDPLFIISTDVETNSIYTGLTSQHPGLFRKALFIEKSEVHWIRTDL
                     ALANGETMEVMARIRYRQPLQKATLHQFEGGMYVAFDEPQSAITEGQFVAWYLEDELV
                     GSGVIS"
BASE COUNT          875 a          627 c          506 g          922 t
ORIGIN      
        1 tgtcgaagcg gcaaatcgac ttcctacttg cactccttcg gctccaagaa ccattgcggc
       61 tagcattcca cgaccgtttg caattcctcc agcggcaatc aatggaattt tgattttttc
      121 ttttaccatc ggaattaaag taaaagtagt ggtttcctct cttccgttgt gtcctccggc
      181 ttcaaaacct tcggcaacca cagcatcgac tccagcatct tgtgctttca aagcaaaaaa
      241 cgtactgctg acaacgtgaa caaccgttat tcctttttcc ttcaaaaaag gagtccaagt
      301 tttgggattt cctgccgatg tgaaaacaat tttcacccct tcttcgacca gaatagtcat
      361 aatttcctcg atattgggat acaacatcgg aacatttacc ccaaaaggtt tatcagtagc
      421 ttttttgcat ttttgaatgt gttctcttaa aacttcggga tacattgaac ctgcaccaat
      481 caaccccaaa ccgccagcat tgctgactgc actagccaat ttatagccgg aattccaaat
      541 cattccagct tgaataatgg gatatttgat attgaaaagt tgagtgattt tgttcattta
      601 ctgtttgata atttttccac tttcggaaat accattcgat tcaattttat aaaaataaat
      661 gccgctattc aaagatttca atgaaatagt tggcgattgt gtagagatat ttttttctaa
      721 aactttttgt cccaaaatag tataaatagc aattgttcta acctctgacc catttggtaa
      781 agtgaccgaa atggaatcat cagtaggatt tggatagacc ttaaaatcat ttgtagaaaa
      841 agctgcaacc gacaatccat tggataaagc caaactaaaa ttaggaattc cataaccata
      901 ttgagttctt gattttactg ctggttcagc aaaattatcc gaagattgaa cgactaattg
      961 tttgatttgt tgcgcatttt ttccgggtaa agcttgccac aaacaagcca ccattccggc
     1021 taaaatagga caagaatagg aagttccgct gccaccatta ttaattgttc caccagaata
     1081 agtataagga tattgtccct gagccattac atctggtttt actcgaccat catagcttgg
     1141 accaatagaa ctgaacgaag cataagttct atcgtctttc accgcgccaa cggcaataac
     1201 atttaaagct tcggctggaa caccaatgtg tggttctgga ttagcacctt cgtttccagc
     1261 acttgccaac acaacaattc ctttgctaaa agcaacattc gccccttttg aagcaaaagc
     1321 agcatttcca gtcatatcag cataagtgtg accgtaagaa gcatcgtcaa attcaaaata
     1381 gcccaaagaa gttgtgatga tatctacacc tactctatcg gcttcctcgg cggcttctac
     1441 ccaattcgat tcttcaacag gattttcgga agcatcatct tctgtgatgt acaaataata
     1501 ttttgcatcg ggagccgttc ctaccaattg tccatcgaca aaacctccca tcaaggagag
     1561 tacagccgtt ccgtgtgttc ctccagaata aaaatcactg cttttagtca cataattata
     1621 tccacctaag atttgtgtat tatctcttaa tctttgaaat ggcgtagcgg tattcacccc
     1681 aggaaaaccg gaatccaaaa ccgcaattat ttttccagaa cccgtataat tggcttgatg
     1741 taacaaatgt ccgttcagca tttgaacctg attcaaagaa ttgccataat tatagattac
     1801 ggccgtttcc atcgttttat ttacaggagt atattttaat tttgctttag ttttgttagg
     1861 agagctattc aacgttttat cagcaaaatc aacattaagc acaaaagaca ataattttag
     1921 tgcattaata tcggcttgcg aaccccgaac gtgaacgcaa ttgaaccatt tagacttggc
     1981 tttgacacta attccagtcg aagttgctac ttggtcaata taaggttgat aaataggaac
     2041 atctttactg tccaatgcaa ttccttggtt ggaacgtcga tccaaagctc tttgcgaaag
     2101 catcgtcaac ggattactca aataagtagc tgaatcgggt ttgtcgttaa aatacaccca
     2161 agcatcttct tgcgaaaaac caacaaaact cgaaactaaa aacaggaaaa gaattatttt
     2221 tttcatatca attttaggaa ataaaatact aatttaggaa ataactcccg aacctactaa
     2281 ttcatcttct aaataccaag ctacgaattg cccttcggtg atagctgatt gtggttcatc
     2341 aaaggctacg tacattccac cttcgaattg gtgtaaagtt gctttttgca agggttgacg
     2401 gtagcgaatt ctagccatta cttccattgt ttctccattg gctaaagcca aatcagtgcg
     2461 aatccaatgc acttcggatt tttctataaa caacgctttt ctgaacaatc ctggatgttg
     2521 actcgttaaa ccagtgtaaa tagaattggt ttctacatca gttgaaatga tgaacaatgg
     2581 atcggttgtt ccgcccacat tgagcccttt tctttgaccg atggtaaaat aatgtgcgcc
     2641 ttgatgtttg cctatcactt tccccatttc tggagtataa tctatttttt tggcatcgaa
     2701 ggctaatgct tcttcaacag aaagatgggc tggtttctct aaattataaa tcggtgaatc
     2761 tttatctatt tggtaaatga ttccgtcttt gggttgcaat ttttgttgta aaaattcggg
     2821 taaccttact tttccaatga aacacaaacc ttgtgaatct ttcttatcgg cggttaccaa
     2881 atccaattct agagcgattt cacgcacttc gggttttgtt aattcgccta
//
LOCUS       DAOSNN010000284         2923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_33969_length_2923_cov_4.831241, whole genome shotgun sequence.
ACCESSION   DAOSNN010000284 DAOSNN010000000
VERSION     DAOSNN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33969_length_2923_cov_4.831241"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PLQ70_11340"
     CDS             <1..570
                     /locus_tag="PLQ70_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HQE35255.1"
                     /translation="DDLSIPKYATHLIYMTNAGRTDEIEEKVMYSILRKRPKRADIYW
                     FVHVNILNEPYKTEYKVTEIAEDTLFRVDFNLGFREPTKINLMFKEVIKDMVKKGEVD
                     VTSRYESLNKNNIIGDLKFVLSEKYLSNDSDLTFFEKIIMNSYFFIKKLSLSEESAFG
                     LDSGSVKIEKFPMVLHAPENIGLTRINNN"
     gene            684..1898
                     /locus_tag="PLQ70_11345"
     CDS             684..1898
                     /locus_tag="PLQ70_11345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008468472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQE35256.1"
                     /translation="MRLHRNLVYTTIDALNAIFNEGEYADKVVARSLKKDKRWGSSDR
                     KFVAETIYEIVRWKRLYSEIAEVKEPFDRDNLWRMFSVWAVLRGYPIPDWRQLEGTPE
                     RKIKGRFDELSKIRTLKESIPDWMDELGVKELGEKVWAKEIAAQNQPAKVILRTNTLK
                     TTKEQLRAILMDLNIETEYLKDQPDALVLKERANVFLTDAFKQGLFEVQDANSQLVAA
                     FLDVKPGMRVVDTCAGAGGKTLHIAALMENKGQLIAMDLYESKLKQLKLRAKRDGAFN
                     IEYRIIDSTKVIKKLHERADRVLIDAPCSGLGVLKRNPDAKWKLQPEFIDNIRKVQAE
                     VLENYSKIVKPGGKLVYATCSVLPSENQEQVEKFLKTEIGKQFNFIKDQKILAHESGF
                     DGFYMALLERKE"
     gene            complement(1983..2213)
                     /locus_tag="PLQ70_11350"
     CDS             complement(1983..2213)
                     /locus_tag="PLQ70_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HQE35257.1"
                     /translation="MEQDQKQLIKLAHTKMPFGKYEGKYLIDLPEYYVVWYSNKGFPK
                     GELGQQLQLIYELKLNGLEELIRNIKKRYPKP"
     gene            complement(2268..2669)
                     /locus_tag="PLQ70_11355"
     CDS             complement(2268..2669)
                     /locus_tag="PLQ70_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQE35258.1"
                     /translation="MTSKVTYLGDLRTSSIHLQSGSEIISDAPLDNNGKGEAFSPTDT
                     VANALASCAMTVMGIKANSLNVDFTGSTAEVTKVMNAEPRRIGAIEIRFNMVGVADQK
                     DRIILERTAMTCPVFLSLNPEIEKKISFNWK"
     gene            complement(2723..>2923)
                     /locus_tag="PLQ70_11360"
     CDS             complement(2723..>2923)
                     /locus_tag="PLQ70_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HQE35259.1"
                     /translation="TRGFDVTFDTLLRLSQGKSYQETAESTATEQVDNKFEYYKKADG
                     GFTNKGIYILYYDTDLTIKVAN"
BASE COUNT          991 a          480 c          522 g          930 t
ORIGIN      
        1 gatgatttat ccatacccaa atatgctacc catttaattt atatgacaaa tgctggtaga
       61 accgatgaaa tagaggaaaa agtaatgtac tccattttgc gaaaaagacc aaagcgagcc
      121 gatatttatt ggtttgttca cgtaaatatc ttaaacgaac cctataaaac ggaatacaaa
      181 gtaactgaaa ttgctgaaga cacactattc agagtagatt ttaatcttgg tttcagagaa
      241 cccacaaaaa tcaatttgat gtttaaagaa gttatcaaag atatggtgaa aaaaggggaa
      301 gtagatgtta ctagtagata tgaatctttg aataaaaaca atattattgg tgatctcaaa
      361 tttgtgttat ccgaaaaata tttatctaat gatagcgatt tgactttctt cgaaaaaata
      421 attatgaact cttatttctt tatcaaaaaa ctaagtcttt ccgaagaaag cgcttttggt
      481 ttagatagtg gttctgtaaa aatcgaaaaa ttcccaatgg tgcttcacgc tccagaaaat
      541 attggtttaa cgagaattaa taacaactaa tcatttgttc aattataaaa aaacactgct
      601 ttggaagcag tgtttttttt tgtgaaaaat ataaaacaaa cttttaatca tttagtagta
      661 cctttgcacc tcaattattt aaaatgagat tacacagaaa tttagtttac accaccatcg
      721 acgctttgaa tgccatcttc aatgaaggag aatatgctga taaagttgtt gcaagatcct
      781 taaaaaaaga caaacgttgg ggaagctctg atagaaaatt tgttgccgaa accatctacg
      841 aaattgtacg ctggaaaaga ttatattcag aaatagccga agtgaaagaa ccttttgata
      901 gagataatct ttggagaatg ttttctgttt gggcagtctt gagaggttat cctatccccg
      961 attggcgcca attagaagga actcctgaaa gaaaaataaa agggcgtttt gacgaacttt
     1021 caaaaattag aacccttaaa gaatctatcc ccgattggat ggacgaattg ggcgtgaaag
     1081 aattaggcga aaaagtttgg gcaaaagaaa tcgcagctca aaaccagcca gccaaagtaa
     1141 ttctgagaac taacacttta aaaaccacca aggaacaatt aagagccatt ttaatggact
     1201 tgaacattga aaccgaatac ttgaaagacc aacctgatgc tttagtacta aaagaaagag
     1261 cgaacgtttt cttgaccgat gcattcaaac aaggtttatt tgaagttcag gatgccaatt
     1321 cacaacttgt tgctgctttc cttgatgtaa aacctggaat gcgagtggtt gacacttgtg
     1381 caggtgcagg tggaaaaact ttgcacattg ctgctttgat ggaaaacaaa ggccaattga
     1441 ttgcaatgga tttatacgaa agtaaattga agcaattgaa attgagagcc aaaagagacg
     1501 gagctttcaa tattgaatat cgtattattg attctacaaa agtcattaaa aaattacacg
     1561 aaagagccga tagagtttta atcgatgctc cttgcagcgg tttgggagtt ttgaaaagaa
     1621 atccagatgc taaatggaaa ttacaacccg aattcattga caacattcgt aaagttcaag
     1681 ccgaagtttt agagaattat tctaaaattg taaaaccagg tggaaaatta gtttatgcta
     1741 cttgttctgt tttaccttct gaaaatcaag agcaggtcga aaaattcttg aaaaccgaaa
     1801 tcggaaaaca attcaacttc ataaaagatc aaaaaatatt ggctcacgag tctggctttg
     1861 atggttttta tatggctttg ttggaacgaa aagaataagt ttttcaaaag acagattttt
     1921 gtacacagaa taaaaaaaag tgattttcat attgaaaatc acttttttta tgggcaattt
     1981 ttttatggtt ttggataccg ttttttaata tttcggatta attcttcgag accattgagt
     2041 ttcagctcat aaattaattg caactgctgt ccgagttctc cttttggaaa acctttattg
     2101 gaataccata ccacataata ttctggcaaa tctatcaaat atttgccttc atatttacca
     2161 aaaggcattt tggtatgagc caatttgata agttgttttt ggtcttgttc cattttcttt
     2221 ttgactattc caacactgca aactgcgact gtaaactgaa tactagatta tttccaatta
     2281 aaactaatct tcttctcgat ttcgggattt aaactcaaga aaacaggaca ggtcatagcg
     2341 gttctttcga gaattattct gtctttttga tctgcaactc caaccatatt aaacctaatt
     2401 tcgattgctc caattcgtct aggttcggca ttcataacct tggtgacttc ggcggttgaa
     2461 cctgtgaaat caacattcaa actattggct tttatcccca taacagtcat tgcacaacta
     2521 gccaaagcat tggcaacagt atccgttgga gagaatgctt ctccttttcc gttattgtct
     2581 aaaggtgcgt ccgatatgat ttcacttccc gattgtagat gtatggacga tgttcttaaa
     2641 tcgcctaaat aggttacttt tgaggtcatt tttttgaatt atgagttatg aattatgaat
     2701 tatgcaaact gattatggaa cactaattcg ctacttttat ggtcaaatcg gtgtcgtaat
     2761 acaagatata aattccttta ttagtaaagc ctccgtcggc ttttttgtaa tattcaaact
     2821 tgttatcgac ttgttctgtc gctgtagatt cagcagtttc ttgatagctt tttccttgtg
     2881 acaaacgcaa taaggtatca aaagtcacat caaatcctcg agt
//
LOCUS       DAOSNN010000285         2915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_34097_length_2915_cov_4.107692, whole genome shotgun sequence.
ACCESSION   DAOSNN010000285 DAOSNN010000000
VERSION     DAOSNN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34097_length_2915_cov_4.107692"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLQ70_11365"
     CDS             <1..535
                     /locus_tag="PLQ70_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HQE35260.1"
                     /translation="FDLDATKLNNVDPDPSLQNYNKFTPNFGAGLYFHSDKAYVGFSI
                     PNFLQTNRYDDNEVAIFKERINYYLIAGYVFDLKDYIKFKPAVLTKMVEGAPLQVDAS
                     ANFMFYDKLMLGVSYRWSAALSAMVGFQVSDGLYIGYGYDNETTNLKYSNSGSHEIFL
                     RYELFKNNNKITTPRFF"
     gene            547..2496
                     /locus_tag="PLQ70_11370"
     CDS             547..2496
                     /locus_tag="PLQ70_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQE35261.1"
                     /translation="MKNFILLYITIISVFSFNSYGQKNKITSADKKYDSYAYIKAVST
                     YERVAEKGYKSEDMFQKLGNSYYFNGELDKAAKWYEELFKMNPNQESEYCYRYAQSLK
                     AIGQTEKANKMLETFQQKAGNDSRGKLFEKNKNYLDQIKANSGRYKIEDAGINSKYSD
                     YGSAFYGNKLVFSSARDTGSLGQRKHTWTDQHFTNLYTSDLGEELTPGKVNKFSSKIN
                     SKFNESTPVFTKDGKTMYFTRNNYLDGKKGKDGKRITLIKIYKASFENNNWDKITELP
                     FDSDQYSVAHPTLSPDEKTLYFASDMPGTLGQSDLFKVKINEDGSFGVPQNLGAAINT
                     EGRETFPQITDENELYFASDGHPGLGGLDVFVTKIGIDGTIGEIQNVGDGVNSPKDDF
                     AFLIDTKSRRGFVTSNRDGGQGYDDIYKFLETRKLVCLQELNGTVTDLNTAQALPDTK
                     MSLFDSEFKLVNIGVSDAVGKYKFEVECGKTYFVRAAKPDYVVKEKMVTIAKENGRTQ
                     LDIALEKEVCKVAIGDDLGKCFQIKMIYFDLDKYNIRTEAALDLEKILDVLNQNPTMK
                     LDIRSHTDCRQTAQYNQVLSDKRAKSTIAWLVKNGVDPKRLTGRGYGESQLVNNCGCE
                     PTNKSNCTEEQHQMNRRSEFIITAL"
BASE COUNT         1077 a          489 c          505 g          844 t
ORIGIN      
        1 atttgattta gatgctacca aactaaacaa tgtcgatcct gatccaagtt tgcaaaatta
       61 caacaaattt actccaaact ttggtgctgg tttatacttt cattctgaca aggcttatgt
      121 tgggttttcg attcctaatt ttttacaaac caatcgttat gatgataatg aagtagctat
      181 tttcaaggaa agaataaatt attaccttat tgcaggttat gtttttgatc ttaaggatta
      241 cataaaattc aaacccgctg tactcaccaa aatggtcgaa ggagctccac tacaagtgga
      301 tgcatctgcc aattttatgt tttatgacaa actgatgctt ggagtttcct acagatggag
      361 tgccgctttg agtgcaatgg taggttttca agtttcagac ggattgtaca ttggatatgg
      421 atatgacaat gaaaccacta atctaaaata ctctaattcg ggttcgcacg aaatattctt
      481 gcgttatgaa ttgttcaaaa acaacaacaa aatcaccact ccaagattct tctaaaaaca
      541 attattatga aaaattttat actcctttac ataacaataa taagtgtttt ttcatttaat
      601 agttatggtc aaaaaaacaa aatcacttct gcagataaga aatacgatag ctatgcctat
      661 ataaaagctg tgagtaccta cgaaagagta gccgagaaag gatacaagtc agaagatatg
      721 ttccaaaaac taggcaattc ttactacttt aatggcgaat tggataaagc cgccaaatgg
      781 tatgaagaat tattcaagat gaatccaaac caagaatcag aatattgcta tcgctatgcc
      841 caatctctaa aagctattgg acaaacagag aaagccaata aaatgttgga aacatttcag
      901 caaaaagcag gcaacgatag cagaggaaaa ctattcgaaa aaaacaaaaa ttatctagac
      961 caaatcaaag ccaattctgg aagatacaaa atagaagacg ccggaatcaa ctctaaatat
     1021 tctgattacg gaagtgcttt ttatggcaac aaacttgttt ttagttctgc tagagacact
     1081 ggaagtttag gacaaagaaa acatacttgg actgatcaac attttaccaa tctttataca
     1141 tccgatttgg gcgaagaatt aacacctgga aaagtaaaca agttttcgag taaaatcaat
     1201 tctaaattca acgaatcgac cccagttttt acaaaagatg gtaagacaat gtactttacc
     1261 cgaaacaatt atttggatgg aaaaaaagga aaagacggaa agagaattac tttaattaaa
     1321 atttacaaag ctagttttga aaacaacaat tgggacaaaa taaccgaatt gcctttcgat
     1381 agcgatcaat acagtgtagc acatccaacg ctaagtccag acgaaaaaac attgtatttt
     1441 gcctcagata tgcctggaac tcttggtcag tcggatttat tcaaagtcaa aataaatgag
     1501 gacggcagct ttggtgtacc ccaaaatcta ggtgcagcaa tcaataccga aggaagagaa
     1561 acttttcctc aaatcacaga cgaaaacgaa ctctattttg cctcagacgg acatccaggc
     1621 ttaggcggtt tagacgtttt tgtgactaaa ataggaattg atggaacaat tggagaaatt
     1681 caaaatgtgg gagatggtgt caattcgccc aaagatgatt ttgctttttt aatagacacc
     1741 aaatccagaa gaggttttgt tacctccaat agagacggag gtcaaggata tgatgatatc
     1801 tacaaatttt tagaaaccag gaaattagtt tgtcttcaag aattaaatgg aaccgtaacc
     1861 gatttgaata ccgcgcaagc acttcctgat acaaaaatga gtttgtttga tagtgaattc
     1921 aagctagtaa atataggagt ctcggatgca gttggtaaat acaaatttga ggtagaatgt
     1981 ggcaaaacct actttgtaag agcagcaaaa ccagactatg ttgtgaagga aaaaatggtt
     2041 acaattgcaa aagaaaacgg aagaacccaa ttggacattg cactagaaaa agaagtgtgc
     2101 aaagtagcca ttggagatga tttaggaaaa tgtttccaaa tcaagatgat ctatttcgac
     2161 ttggataaat acaatattcg aactgaagct gctttggatt tagaaaaaat attagacgtt
     2221 ttgaatcaaa atccaacaat gaaattagac attcgttctc ataccgattg ccgacaaaca
     2281 gcccaataca atcaagtttt atcagacaaa agagccaaat cgaccatcgc ttggttagtg
     2341 aaaaatggtg ttgatcctaa aagattgaca ggcagaggat atggtgaatc gcaattagta
     2401 aacaattgtg gttgcgaacc aaccaacaaa tccaactgta ccgaagaaca acaccaaatg
     2461 aacagacgta gcgaatttat tattactgct ctgtaaaaac atccaaaaga tattaatcaa
     2521 aaccctcgaa agcgaatgcc gtcgaggttt ttttttctat ttttagagta gttttataag
     2581 ttatttagag acctattttc gctctatttc tttagataaa tcttgttgtt tttcattcat
     2641 catttttgtg acagtaatgt gcatccaaat caaaattatc aggttgacat taaatcaaaa
     2701 aaagcctttg aactcaatca aaggcttctt actttttact aatttctatt ttaattttga
     2761 aaaattgatt cgaatttaaa agtcaaatct atgccaacac tacttgaggt ttctttacat
     2821 cttttactgc taatttcaca gctaaaacaa caactccagt tattagaatg ataattccta
     2881 atataaaaaa agcattggta taatctatta aatct
//
LOCUS       DAOSNN010000286         2860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_34972_length_2860_cov_6.048128, whole genome shotgun sequence.
ACCESSION   DAOSNN010000286 DAOSNN010000000
VERSION     DAOSNN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34972_length_2860_cov_6.048128"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLQ70_11375"
     CDS             <1..527
                     /locus_tag="PLQ70_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35262.1"
                     /translation="INKSVTLGTGLNLEQKIIHYNTDFTVDYISELGIIERDVINAET
                     NNKYKYLSVPLFLKYCFGHNKSYFVSGGVFISFLQNYQKESSFVNKSGGSILYSSNSI
                     YNFPLAEDVSGDYGVSFGFGKSFELNDKNKISIEIRDNLGLQNTVDRTFNYGISGVIK
                     TNCLNLIAGWSFDL"
     gene            632..1297
                     /locus_tag="PLQ70_11380"
     CDS             632..1297
                     /locus_tag="PLQ70_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35263.1"
                     /translation="MKKYILVLGILAFLSCKRAEEPRGITFYFENPQPINDAELSKIP
                     NKFQGLFVNSDSVFLNIKENIILKEYFNRFKFHKKEFDSLKLDFDQVGDKYISKFNQD
                     VYVAKFLKDSIEFSNKRVDTFFVFSNTHKAKRFDGKLVLNDKDSIFWKIKSISLEKNR
                     LKIKFIYSKEDLKRMDSLTKIKSTMIDSSSFIIKPSRNEFKRILNLKKLGGEFEYRKV
                     QNN"
     gene            1319..1840
                     /locus_tag="PLQ70_11385"
     CDS             1319..1840
                     /locus_tag="PLQ70_11385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQE35264.1"
                     /translation="MKIIAFAGSPSKNSINKKLATYASSLFANAELEVLDLNDYEMPL
                     FSVDKEAVIGQHPLAKAFLDKIGSADFLVVSLAENNGNYSAAFKNLVDWCSRINGKIF
                     QEKSMLLMATSPGGRGGASVLEIAKNNFPRFNADIKAVFSLPSFNDNFDVEKGMISNK
                     ELDDQLKEIIKGL"
     gene            1896..2579
                     /locus_tag="PLQ70_11390"
     CDS             1896..2579
                     /locus_tag="PLQ70_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HQE35265.1"
                     /translation="MQSVKNSLKIIGWTLLGIISFFILYIISALSISKISVNSDVSQK
                     DKPIEIFILSNGVHTDIVVPIKNEIKDWSKEIRFHQTKSKDSLVHYLAFGWGDKGFYL
                     NTPEWSDLKASTAFNAAFGLSSSAMHTTFYKKMNENASCKKIKISSQEYQKLITYISE
                     SFHRDLHKNVQWISGHSYGNRDAFYEAKGSYNLFYTCNTWANNALKIANQKASLWTVT
                     DTGIFCQYQ"
BASE COUNT         1002 a          382 c          496 g          980 t
ORIGIN      
        1 gaataaataa aagtgtaact cttggtactg gcttgaattt agagcaaaaa ataattcatt
       61 ataataccga ctttacagtt gattatataa gtgaattggg gattattgaa agggatgtta
      121 tcaatgcaga aacaaataat aagtataagt atctttcagt ccctttattt ttgaaatatt
      181 gctttggtca taacaaatct tattttgtta gtggaggtgt ttttatttcg tttttgcaaa
      241 actatcaaaa agagtcaagt tttgtaaata aaagtggagg tagtatcctt tactcctcta
      301 attcaatata taattttcct ttagctgaag atgtttcggg agattatggg gtttcttttg
      361 gatttggtaa atcatttgaa ttaaatgata agaataaaat ttcaatagaa attagagata
      421 atttaggatt acaaaacaca gttgatcgta cttttaatta tggtatatcg ggagttatta
      481 aaactaactg tctaaattta attgccggtt ggtcttttga tttgtagaaa aaatatctca
      541 ttttttataa aactgttcaa gcaattgggc agtttttttt tgtttaattt gtgtttcaat
      601 ttttattact tttagtctaa agaaaataca aatgaaaaaa tatattttag tgttgggaat
      661 tctagctttc ctttcttgca aaagagccga agagccaaga ggaattactt tttatttcga
      721 aaatcctcaa cccataaatg atgcggaatt gtccaaaatt ccaaataaat ttcagggatt
      781 gtttgtgaat tctgattctg tctttttgaa tattaaagag aatattattt taaaagaata
      841 ttttaaccgg ttcaaatttc ataaaaagga gttcgattct ttaaaactgg attttgatca
      901 agtaggggac aagtatattt caaaattcaa tcaagatgtt tatgtggcta aatttctaaa
      961 agattccatt gaattttcaa acaaacgagt tgatactttt tttgtttttt cgaataccca
     1021 taaagccaaa cgatttgatg ggaaattggt tcttaacgac aaggattcca ttttttggaa
     1081 aataaaatca attagtttgg aaaaaaacag actcaaaatc aaattcatct attctaaaga
     1141 agatttaaaa agaatggatt cgttgacaaa aataaaatca acaatgatag attcttcgtc
     1201 ttttataatt aaaccttcaa gaaatgaatt taaacgtatt ttaaatttaa aaaaacttgg
     1261 cggagaattt gaatacagaa aagtgcaaaa caattaatct agcttaaaac aaaaaaatat
     1321 gaaaatcata gcctttgcag gaagccccag caaaaactcc atcaataaaa aattagcgac
     1381 ttacgcttcc tcattgtttg caaatgccga attagaagta ttggatttaa acgattacga
     1441 aatgccgttg ttcagtgtag ataaagaagc tgtaattggg caacatcctt tggcaaaagc
     1501 atttttggat aaaattggaa gtgctgattt tttagttgtt tcccttgccg aaaacaacgg
     1561 taattattcg gcagcattca agaatcttgt ggattggtgt tcgagaatca acggaaaaat
     1621 atttcaggaa aaatcaatgc tcttgatggc aacttctcct ggaggcagag gcggagcgag
     1681 tgttcttgaa attgccaaaa acaattttcc tcgttttaat gccgatataa aagctgtttt
     1741 ttctttgccc agtttcaacg ataattttga tgtggaaaag ggtatgattt caaataaaga
     1801 attagatgat cagttgaagg aaatcattaa gggtttgtaa aaaaattttt ggctcgaaaa
     1861 ttgttttctg ttttttgaac acaaaaacta ttcgtatgca atcagtaaaa aacagcctaa
     1921 aaattatagg ttggaccctt ttaggaataa ttagtttctt tattttatac attatctcgg
     1981 cactatcaat ttctaagatt tcggtcaatt ctgatgtttc tcaaaaagat aagccaattg
     2041 aaatattcat tttgtcgaat ggagttcata cggatatcgt ggtgccaata aaaaatgaaa
     2101 ttaaagattg gtcgaaagaa attcgttttc accaaacaaa atcaaaagat agtttagtcc
     2161 attatttggc ttttggttgg ggtgataaag gcttttatct caatactccc gaatggtctg
     2221 atttgaaggc gagtacggca ttcaatgcgg cttttgggtt gagttcatct gcaatgcata
     2281 ccacatttta caaaaaaatg aatgaaaacg cgagttgcaa gaaaatcaaa atttcttctc
     2341 aagaatatca aaaattaata acttatattt ctgaaagttt tcatcgtgac ttgcataaaa
     2401 atgttcaatg gatttcgggt catagttatg gaaatagaga tgctttttat gaagctaaag
     2461 gcagttataa cttgttttac acttgtaata cctgggctaa taatgcctta aaaattgcga
     2521 atcaaaaagc aagtttgtgg acagtaacgg atacggggat tttttgtcag tatcaataaa
     2581 tattatttgt atttgctaat tactttgtca ataactttga tatttcccat tttttaattt
     2641 tgtcaaattt cattatatta gcacaaaatc acaaaattta aatgtccgaa caaaatcaat
     2701 ataccgaaga taacatacgt tccctcgatt ggaaagaaca cataagaatg cgtcccggaa
     2761 tgtacatcgg gaaattggga gacggctcct cgccagatga tggtatttat attttggtta
     2821 aagaagtcct cgataactgt atcgatgaat ttgcgatggg
//
LOCUS       DAOSNN010000287         2850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_35136_length_2850_cov_4.413596, whole genome shotgun sequence.
ACCESSION   DAOSNN010000287 DAOSNN010000000
VERSION     DAOSNN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35136_length_2850_cov_4.413596"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..652
                     /locus_tag="PLQ70_11395"
     CDS             47..652
                     /locus_tag="PLQ70_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQE35266.1"
                     /translation="MKEKEGYLIRDQSKAHFITATVVDWVDVFSRKTYKDCVIESLDF
                     CIKNKGMILYGFVIMSNHIHLIIQSEDSKLSDLIRDFKKFTAKTILNKIETEPESRAD
                     WMLKRFEFACKSHNRNEKYQFWQYGNHPEEIFSEKFFWSKLDYIHLNPVRAGIVSKAS
                     HYVYSSASNYVEDSGIMAITKVDNPVIDVLKPQSISRIYNW"
     gene            complement(711..2000)
                     /locus_tag="PLQ70_11400"
     CDS             complement(711..2000)
                     /locus_tag="PLQ70_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQE35267.1"
                     /translation="MSFNSLGLSDALLKAISKKGYSTPSPIQQKAIPPILEGKDVLAS
                     AQTGTGKTAGFTLPILQLLSQGKHLSHRPIRALILTPTRELAAQILANIKEYSEFVNL
                     RSAVIFGGVNQNPQVSQLRQGIDILVATPGRLIDLQNQGLISLSKVEILVLDEADRML
                     DMGFLRDIERILKVLPPKRQNLLFSATFSKDIKKLAMGILHHPVQVEATPENTTVDAI
                     IQKVYPCAKEKKTELIIKLITEGNWKQILVFTRTKQGANKLTESMISSGIRAAAIHGN
                     KGQGARTKALAGFKDGSLTALVATDIAARGLDIPLLPHVVNFELPNIPEDYVHRIGRT
                     GRAGASGEAISLFSPDETVFLRDIEKLVGLKLPKENIKGFEPDPNASTEPIKQGQGRQ
                     QRNSTPRKPNTDNSNRSSNNSFGPRRPQQNNDRRSNR"
     gene            complement(2119..2625)
                     /locus_tag="PLQ70_11405"
     CDS             complement(2119..2625)
                     /locus_tag="PLQ70_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020212567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQE35268.1"
                     /translation="MNYHFRKAGTTDIPEIWAILQQAIIRRKNDGSNQWQDGYPNPEV
                     IKKDIEKGYGYVLTEAEKTIGYCAILINDEPEYQKIEGEWLTDSDFVVFHRVAISENY
                     LGKNLSKKIIEYIEDFARKNNILSLKADTNHDNFAMMKIFEKSGFTFCGIVHFRGSPR
                     RAYEKVLE"
     gene            complement(2678..>2850)
                     /locus_tag="PLQ70_11410"
     CDS             complement(2678..>2850)
                     /locus_tag="PLQ70_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HQE35269.1"
                     /translation="LSAANAVGDDAIQKKMQGQIVPDSFTHGTSEQRMYWFNRGFKLG
                     DINKGDTFSEVD"
BASE COUNT          799 a          515 c          555 g          981 t
ORIGIN      
        1 tgaagtagtg attttcagtc tctgactgac ctttaacaaa gacaaaatga aagagaaaga
       61 aggataccta attcgagatc aatccaaagc gcattttata accgcaacgg ttgtggactg
      121 ggttgatgtt ttttctagaa aaacctataa agattgcgtc atcgaaagtc tggatttttg
      181 tataaaaaac aaaggaatga ttttgtatgg gtttgtaatt atgtccaatc acattcattt
      241 aataattcag tcagaagaca gtaaattatc cgatttaatt cgggatttca agaagtttac
      301 ggcaaagacc attttgaata aaatagaaac cgagccagaa agtagagccg attggatgct
      361 gaaacgtttt gaatttgctt gcaaaagtca taaccgaaat gaaaaatatc agttttggca
      421 atatggaaat catcccgaag aaattttttc agaaaagttc ttttggtcga aattagatta
      481 tattcatttg aatcctgttc gtgcaggaat tgtttccaaa gcttctcatt atgtttattc
      541 cagtgcttcc aattacgttg aagattctgg aattatggca attacaaaag tggataatcc
      601 agtaatagat gttttgaaac cacaatcgat tagcagaatt tataattggt aacgaaggtc
      661 agagaccttt tatcacatcc acaaagtcgg agactttgcg gagcataaaa ttatctatta
      721 gaacgcctat cattattttg ttggggacgt cttggaccaa agctgttatt actgcttcta
      781 tttgaattat ctgtattggg tttccttgga gttgagttgc gttgttgtct tccttgtcct
      841 tgtttgattg gttctgttga agcgttaggg tctggctcaa aacctttgat gttttctttc
      901 ggcaatttca agcccaccaa tttttcgata tcacgtaaaa aaacggtttc gtctgggcta
      961 aaaagcgaga tggcttctcc acttgctcca gctcttcctg tacgaccaat tcggtgcacg
     1021 taatcttcgg gaatattggg caattcaaaa ttaactacgt gtggcaataa tggaatatcc
     1081 aaaccacgag cggcaatatc ggtagcgacc aaagcggtta ggcttccatc tttgaaaccc
     1141 gctaaggctt tggttcgtgc gccttgacct ttgtttccgt gaatcgctgc tgctctgatg
     1201 ccagaactaa tcatactttc ggtcagtttg ttggcacctt gtttggttcg tgtgaatacc
     1261 aaaatctgtt tccagttgcc ttccgtaatc agtttgatga ttaattcggt ttttttctcc
     1321 ttggcacaag gatatacttt ttggataatg gcgtcaacgg ttgtgttttc gggtgtggct
     1381 tctacctgaa cgggatggtg caaaatgccc attgccaatt tcttgatgtc tttggaaaag
     1441 gttgccgaaa atagcaagtt ttgtcttttt ggcggtagta ctttcaagat gcgttcgata
     1501 tcgcgtaaaa atcccatatc cagcattcga tcggcttcat ccaaaaccaa aatctccact
     1561 ttggaaagtg atatcaaacc ttgattttgc aaatcgatta atcgaccagg tgttgccact
     1621 aaaatatcaa tcccttgacg caattgactt acttgtgggt tttggtttac gcctccaaaa
     1681 ataacggcag agcgtaaatt tacaaactca ctgtattctt ttatgttggc caatatttgt
     1741 gctgccaatt ctcgtgttgg agttaatatc aaggcacgaa taggtcggtg acttagatgt
     1801 tttccttgcg ataataattg caaaataggt agagtgaaac ctgctgtttt tcctgttcct
     1861 gtttgggcag atgccaaaac atccttgcct tccaagatgg gtggaattgc tttttgttgt
     1921 attggagaag gggttgagta tccttttttg ctgatggctt ttaataaagc atccgataat
     1981 cctaatgagt tgaatgacat aaatggtgtt tgatgaagta aacactattt atatggagta
     2041 cttctttaat tgggcgcgaa ggtacagtaa atttatggat gtggttttga ttgcttgatt
     2101 ttgattcaaa agtaatgatt attcaagcac tttttcgtag gctcttcttg ggcttcctct
     2161 gaaatggact ataccacaga aagtaaaacc cgatttttca aaaatcttca tcattgcaaa
     2221 attatcgtgg ttggtgtcag cctttaaact cagaatatta ttttttcgag caaaatcttc
     2281 gatgtattca ataatttttt ttgatagatt tttacccaaa tagttttcgg aaatggctac
     2341 acggtgaaaa acaacaaaat ctgaatctgt taaccattcc ccttcaattt tttggtattc
     2401 tggttcgtca ttgataagga tggcacaata accaatagtt ttttcagctt cggtcaaaac
     2461 atatccatag cctttttcaa tatctttttt tatgacttct ggatttggat aaccatcttg
     2521 ccattgattg ctgccgtcat tttttcttct tataatagct tgttgtaaaa tagcccaaat
     2581 ttcaggaata tctgtggtac cagcttttcg aaagtgataa ttcataatgt ttttttggaa
     2641 tattaatttt cgaatattta cctacttttc attctcccta atccacctcc gaaaaagtat
     2701 cccctttatt aatatccccc aatttaaaac cgcgattgaa ccaatacatt ctttgttcgg
     2761 aagttccgtg ggtaaaggaa tcaggaacga tttgtccttg catttttttc tggatggcgt
     2821 cgtcacctac tgcatttgct gcactcaaag
//
LOCUS       DAOSNN010000288         2789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_36132_length_2789_cov_5.542794, whole genome shotgun sequence.
ACCESSION   DAOSNN010000288 DAOSNN010000000
VERSION     DAOSNN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36132_length_2789_cov_5.542794"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLQ70_11415"
     CDS             <1..211
                     /locus_tag="PLQ70_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQE35270.1"
                     /translation="DDYKVSETMMNYFANFIKSGNPNGEKLPVWPMAKDEKSPEIMVI
                     DIESKATKAENDARYLFLEKEYTKK"
     gene            415..861
                     /locus_tag="PLQ70_11420"
     CDS             415..861
                     /locus_tag="PLQ70_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35271.1"
                     /translation="MEKQVGIWIDTKKAIIVALEGQKEEKITEIDSEVENRIYHNKES
                     NKGTFSASHQSDSETKFDNRKKEQMDYYLEAVVNHIKKADYLYIFGPADTKLKLEQKI
                     RDEKSLGKIKLKAVETVDKMTLNEIVAQVKDFYNPLKRKLNLAHRL"
     gene            1022..>2789
                     /locus_tag="PLQ70_11425"
     CDS             1022..>2789
                     /locus_tag="PLQ70_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQE35272.1"
                     /translation="MRTRKQKIILAVKIVAISFLALIIAVFVFRNELLKQTIAKVSFK
                     MASEYDSTFTVKEASFVGFSGLSFSEIILAPKNADTLFNIQKMKTSVNLWRLLVGDVQ
                     LGTLEIEKGYVQLVKNKKGRNFDAFLKKDETTTSSNEKRDYAKVAYSLISKVLNLVPT
                     DMKVENLSFRLDDNGKKTTINFQKLRLVNKQLETSIKVETKAFIQRIRIEGFADPRNK
                     TADIRFFNMDTGAIKVPYLDERFGLKSSFDSIRVNIQNIDKSGSELHLDGFASIANLM
                     INHKKIANKDVVIKNAKFDYRFLLGSDFISIDSSSTVQFNKIKFHPYLAYETEEDTIY
                     KLKVAIPKMKAQDFIVSLPDGLFTHFQGMEAEGNFEYNLDFKFNKNKPNRLVFDSKLK
                     KENLKITKYGEANLNKLNGEFVYRAIIQNVLQRPVLVGVENPNYTPLDQISPYLQKCV
                     LTSEDPSFFSHRGFINEAFKQSILKNIRTKKFSRGASTISMQLIKNVFLTREKTLSRK
                     LEEILLVYILENNRIASKERMLEVYFNIIEWGPDVYGIGEAAHFYFQKTPADLTLKEC
                     LFLATIVPKPKKFMWQFDNEGNL"
BASE COUNT         1030 a          478 c          471 g          810 t
ORIGIN      
        1 agacgattat aaagtttcgg aaactatgat gaattacttt gccaacttta ttaaatccgg
       61 aaatccaaat ggcgaaaaat tacccgtttg gccaatggca aaagacgaaa aaagtccaga
      121 aattatggtg attgacatag aatcgaaagc aacaaaagct gaaaatgatg cccgatactt
      181 atttttagaa aaagaataca cgaaaaaata ggttttttat aaaaaatatt taaaaaaagg
      241 tggcttcatt taattgaaaa cacctttttt tatttttatt ttcaattgaa attcaataga
      301 ttaatttaaa attttaacga ataaaaaatt gggtggctcg tgaatatttc atcttttctt
      361 cgtaaattta tatgttcatt tttctccaac gaaaagaatt taaaaactaa aatcatggaa
      421 aaacaagtag gaatctggat tgacactaaa aaagcaatta ttgttgcact tgaaggtcaa
      481 aaagaagaaa aaatcactga gatagattct gaagttgaaa acagaattta ccataacaaa
      541 gaaagcaaca aaggaacttt ctctgcaagc caccaaagtg acagcgaaac gaaatttgac
      601 aaccgcaaaa aagagcaaat ggattattat ctggaagctg ttgtgaacca cattaagaaa
      661 gctgattatc tctatatttt tggtccagca gatacaaaac tgaaattgga acaaaaaata
      721 agagacgaaa aatctcttgg caaaattaaa ctaaaagcag tagaaacagt ggataagatg
      781 actttgaacg agattgtagc tcaggttaaa gacttttaca atccactgaa acgtaaactt
      841 aatttggcgc atcgactata aattttaaac ttttaaaacc atcgaaaacc acaatacaaa
      901 aatgttgtgg tttttttgtg tcgggaaagc aacaaaactc taaagagaat cataaaatta
      961 attcccattc cttttttttt cttttcttta tgctccgaaa aattgataca gaaactagtt
     1021 tatgagaacc cgaaaacaaa aaattatttt agctgtaaaa atagttgcta tatctttttt
     1081 ggcactaatt atagctgttt ttgtttttag aaatgaattg ttgaaacaaa ccattgccaa
     1141 ggtttctttt aaaatggcaa gcgaatatga cagtactttc actgtaaaag aagcttcttt
     1201 tgttgggttt tccggtctta gtttttccga aataatttta gctcctaaaa atgccgacac
     1261 gcttttcaat atccaaaaaa tgaaaaccag cgtcaattta tggcggctgt tggttggcga
     1321 tgtgcaatta ggaacattgg aaatcgaaaa aggatatgtg caattggtca aaaataaaaa
     1381 aggacgaaat ttcgatgctt tcctaaaaaa agacgaaacc acaacttcct ccaacgaaaa
     1441 aagagattat gccaaagtag cctatagtct catctcgaaa gtgctgaatt tggttccaac
     1501 ggatatgaaa gtggaaaacc tttctttccg attggacgat aatggcaaaa aaactacgat
     1561 taatttccaa aaactacgat tggtcaacaa gcaattagaa acttccatca aagtcgaaac
     1621 caaagctttc atacagcgaa tccgaattga aggttttgcc gacccaagaa acaaaactgc
     1681 cgatattcgg ttttttaata tggataccgg agcgataaaa gtaccttatt tagacgaacg
     1741 ttttggctta aaatcaagtt tcgattccat tcgagtaaac attcaaaaca tagacaaatc
     1801 gggtagcgaa ttgcatcttg acggctttgc ttcgattgcc aatttgatga tcaatcacaa
     1861 gaaaattgcc aataaagatg tggttatcaa aaacgcaaaa ttcgattatc gattcttgtt
     1921 gggttccgat tttatttcta ttgacagtag ttctacagtg caattcaaca aaataaaatt
     1981 ccatccttat ttggcttatg aaactgagga agacacgatt tacaaactca aagtagccat
     2041 tccaaaaatg aaagcgcagg attttattgt gtcgcttccc gatggtttgt tcacgcattt
     2101 tcagggaatg gaagccgaag gcaatttcga atacaatttg gatttcaaat tcaataaaaa
     2161 caagcccaat cgattggttt ttgatagcaa gttgaagaaa gaaaacctaa aaatcaccaa
     2221 atacggcgag gccaacctaa acaaactcaa tggcgaattt gtgtatcgag ccatcattca
     2281 aaatgtattg cagcgacccg ttttggtagg agttgaaaat cccaattata cgcctttgga
     2341 tcaaatatcg ccttatttgc aaaaatgcgt tttaacttct gaagaccctt cttttttctc
     2401 acatcgtggt tttatcaacg aggctttcaa acaatcgatt cttaaaaata ttcgcaccaa
     2461 gaaattctct cgtggggcta gtaccatcag tatgcaatta atcaaaaacg tatttcttac
     2521 ccgcgaaaaa accctttctc gaaaactaga agaaatcctc ttggtttaca ttcttgaaaa
     2581 taatagaatc gccagcaagg aaagaatgct ggaagtctat ttcaacatca tcgaatgggg
     2641 tcctgatgtc tatggcattg gcgaagcagc ccatttttat ttccaaaaaa caccagccga
     2701 tttaacccta aaagaatgtt tatttctagc cacgattgtt ccaaaaccaa agaaatttat
     2761 gtggcaattt gacaacgaag gcaacttaa
//
LOCUS       DAOSNN010000289         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_36201_length_2785_cov_3.790842, whole genome shotgun sequence.
ACCESSION   DAOSNN010000289 DAOSNN010000000
VERSION     DAOSNN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36201_length_2785_cov_3.790842"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLQ70_11430"
     CDS             complement(<1..347)
                     /locus_tag="PLQ70_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35273.1"
                     /translation="MKSTKKIIAAIIILFSINTISAQYGNGYGNGYGNGYGYGNGMSQ
                     MGGMNQNSQPEKPKEVPAEVIVAKYMEDMKPALALDELQTIAITNVLIESVNAQGRIT
                     KLNLPQEDLINEY"
     gene            complement(372..1673)
                     /gene="rimO"
                     /locus_tag="PLQ70_11435"
     CDS             complement(372..1673)
                     /gene="rimO"
                     /locus_tag="PLQ70_11435"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQE35274.1"
                     /translation="MRTKSLKKNKINVITLGCSKNVYDSEVLMGQLKASGKDVVHEEE
                     GNIVVINTCGFIDNAKAESVNMILEYADKKERGIVDKVFVTGCLSERYRPDLEKEIPN
                     VDQFFGTTELPALLKALGADYKHELLGERLTTTPKNYAYLKIAEGCDRPCSFCAIPLM
                     RGKHVSQTIEKLVKEAEGLAKNGVKELILIAQDLTYYGLDIYKKRNLGELLEALVKVE
                     GIEWIRLHYAFPTGFPMDVLEIMKREPKICNYLDIPLQHISDSILKSMKRGTTQEKTT
                     KLLKEFREAVPGMTIRTTLIVGYPGETQEDFEILRDWVQEMKFERLGCFAYSHEENTS
                     AFELVDDVPEDVKQDRANEIMELQSQISWDLNQEKIGKTFRCIIDRKEGEHFVGRTEF
                     DSPDVDNEVLIDASKHYVKTGDFVMVKIIDATEFDLFAEPV"
     gene            complement(1823..>2785)
                     /locus_tag="PLQ70_11440"
     CDS             complement(1823..>2785)
                     /locus_tag="PLQ70_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQE35275.1"
                     /translation="LGYEGYIINPVNENSNNTAYTSNVTPGGNYYQENSIYSSGYNGK
                     LSFNISTSNENKFYIGANLNSHFTDYVRTSSFYESNNNPLNTDYTVTNLRFDNNLHTF
                     GTGFSFQIGAIAKVTDEIRLGLAYDSPIWYKLSDEFSQKLVAISANTVNQLPPDVVDP
                     NIINYYDPYKLTTPSKFTGSFAYVFGKKGLISLDYSLKNYGNTKFEPEYDYYYSGLNN
                     EMKNTLKSSGELRLGAEYKINQWSLRGGYRFEESPYKNGATIGDLNSFSGGLGYDFGM
                     FKLDLSYTNAQRNTQQPFFNQGFTDSAKINTTNNNFTMTLLFEM"
BASE COUNT          826 a          503 c          430 g         1026 t
ORIGIN      
        1 ttatattcat ttattaaatc ctcttggggt aaatttaatt ttgtaattct gccttgtgca
       61 tttacacttt caatcaaaac atttgttata gcgatggttt gcagctcatc aagtgctaaa
      121 gcaggtttca tatcctccat gtatttggcg acaattactt ctgcgggaac ttcttttggt
      181 ttttcgggtt gactattttg attcatacct cccatttgac tcattccatt tccatagcca
      241 taaccatttc cgtaaccatt accatagcca tttccatatt gggctgatat cgtgtttata
      301 gaaaagagaa taatgatagc tgcaataatt ttttttgtac ttttcatgtc ttgagtatta
      361 atgatgtaaa tttaaacagg ttctgcgaat aaatcaaatt cagtggcgtc aataattttt
      421 accattacaa aatcacctgt tttgacataa tgctttgaag cgtcaataag tacttcgtta
      481 tcaacatctg gactgtcaaa ttctgttcgt cccacaaagt gctcgccttc ttttctatca
      541 ataatacaac ggaaggtttt tccgattttt tcttggttca aatcccaaga aatttgagat
      601 tgcaattcca ttatttcatt tgcacgatct tgttttacat cttcaggaac atcatcgact
      661 aattcgaatg cgcttgtgtt ttcttcgtgc gaataagcaa aacaacccaa acgctcaaat
      721 ttcatttctt gaacccagtc tctcaaaatt tcgaaatctt cttgagtttc tccaggataa
      781 cctacaatta gtgttgttct gatggtcatt ccaggaacgg cctcccgaaa ttcttttaat
      841 aatttcgtcg ttttttcttg cgtcgttcca cgtttcatcg atttcaaaat agaatccgaa
      901 atgtgttgca acggaatgtc taaataatta caaatcttag gttcgcgttt cataatttcc
      961 aaaacatcca tcgggaaacc agttgggaag gcataatgca gacgaatcca ttcgatgcct
     1021 tctactttta ccaaagcttc taataattct ccaagattgc gttttttgta aatatctaac
     1081 ccgtagtaag tcaaatcttg cgcaatcaaa attaattctt ttacaccatt ttttgccaaa
     1141 ccttcggctt ctttaactaa tttttcgatg gtttgcgaaa cgtgttttcc tctcattaat
     1201 ggaatcgcac aaaaactaca tggtcggtca caaccttcgg caatttttag gtatgcataa
     1261 ttttttggag ttgttgtcaa acgttctcct agcaattcgt gtttgtagtc tgctcctaaa
     1321 gcttttaata aagcaggtaa ttctgttgtg ccaaaaaact gatctacatt tggaatttct
     1381 ttttctaaat ctggacggta tcgttctgat aagcatcctg ttacgaatac tttgtcaaca
     1441 attccacgtt cttttttatc agcatattcc aaaatcatat ttacagattc ggctttagca
     1501 ttatctataa atccgcaggt gttgataact acaatgttgc cttcttcttc gtgaacgaca
     1561 tcttttccgc tagcttttaa ttgtcccatt aagacttcgc tatcatatac attttttgaa
     1621 cacccaagag tgatcacgtt gattttattt ttttttaacg acttggttct catagattta
     1681 tatttccgat aatttagaag ttggcaaaat tacgattttt atttttagga gcataactct
     1741 ttgtatagta atggttttat aaaaacaaaa aaccacttca aaaatgaaat ggttttctat
     1801 tcgaaaattt ttataaaaag ggttacattt caaataataa agtcattgtg aaattattgt
     1861 ttgtagtatt gattttagct gaatccgtaa atccttgatt aaagaaaggt tgttgcgtat
     1921 ttctttgtgc atttgtgtat gaaaggtcta atttaaacat tccaaagtca taacctaatc
     1981 caccagaaaa ggagtttaaa tcgccaatag ttgcgccgtt tttataagga ctttcttcaa
     2041 aacgataccc acctctaaga ctccattgat tgattttgta ctcggctcca agtcttaatt
     2101 cgcctgaaga ttttagagta tttttcattt cattattcaa accgctataa taataatcat
     2161 attcaggttc gaatttagta tttccataat tttttaaaga ataatcaagg cttattaaac
     2221 ctttttttcc aaagacatag gcaaaacttc ccgtaaattt acttggagtg gtcaatttat
     2281 aaggatcata ataattaatt atattcggat caacaacatc aggaggtaac tgatttacgg
     2341 tattggcact tatagcgact agcttttgtg aaaactcatc acttaattta taccaaatag
     2401 gggaatcata ggctaatcct aaacgaatct catcggtcac ttttgctatg gctccaattt
     2461 gaaatgaaaa cccagttccg aaggtgtgca aattattgtc aaaacgcaaa ttggttacgg
     2521 tatagtctgt gtttaatgga ttgttgttgg attcataaaa actgcttgtg cgaacataat
     2581 cggtaaaatg tgaatttaaa tttgctccta tataaaactt gttttcattg gaagtggata
     2641 tattgaagga taattttcca ttatatccgc tagaataaat gctgttttct tgataataat
     2701 ttcctccagg agtcacattt gatgtgtaag ctgtgttgtt tgaattttca ttaacaggat
     2761 taataatata tccttcatag cctaa
//
LOCUS       DAOSNN010000290         2712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_37508_length_2712_cov_5.517877, whole genome shotgun sequence.
ACCESSION   DAOSNN010000290 DAOSNN010000000
VERSION     DAOSNN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37508_length_2712_cov_5.517877"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="PLQ70_11445"
     CDS             <1..1251
                     /locus_tag="PLQ70_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017497238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQE35276.1"
                     /translation="NFDDLNYDVKAKGTLDISKIYKVFSQKGLDVDGSIKADLALKGK
                     QSDAEKGNYSKLNNKGTLELRNILIASEYLPKKFLIKEGIFKINQDKMTFNNFLAAYG
                     QSDFRMNGYLQNVINFATTNTGVLRGSFKLNSTYINADEFMSETEVASTNTTNSETKP
                     AATKVETGVIIIPSNLDLKFQANANKINFNELALKDAKGNMIVKKGILTMQNTGFNLI
                     GCTVNMNAKYQASTPKKANFEYDIKASDFDIKKAYNEIKMFREMASAAEKASGTVSLE
                     YKLKGRLNANMEPVYPSLVGNGVVSVKDIKLHGMKMFSAVAKKTNNDGIKNPELSKVE
                     VKSSIKNNIITLERFKFKFAGFRPRIEGTTSLDGKLNIKMRLGLPPLGILGIPMVITG
                     NKDNPKVKIGRKSDDLEETKDSEK"
     gene            complement(1252..2592)
                     /locus_tag="PLQ70_11450"
     CDS             complement(1252..2592)
                     /locus_tag="PLQ70_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQE35277.1"
                     /translation="MNNKNIVDLTRGAITPQIIHFTLPLIIGNFFLLTYNAADSVIVG
                     RFIGAHALAALGAASPVMNIMLFLIVGICLGMSVLMGNFFGNSDLASLKKVISTSLIS
                     GGLFTLFIVILGFFFSHSILTLMNTPSEIIDDATSYLQIIFIGLVFTFIYNIYASTLR
                     SLGNSKVSLYFLGASAVLNIVLDLLFVAVWEQGIEGAAWATVIAEAAAALFCVLYVRF
                     KVPFLRFKNNEFILDRDLLRVIVGYSFVTATQQITLHLGKLLVQGAVNPLGVFAIAAF
                     NAVTRVDDFVMIVQQNISHGITGFLAQNKGKGNFSRIRKGFWVGVKLEVIYSLIMTVI
                     VFVFAKEIMILFMGNGASEVVEAGIQYLQLMAFLYLLPGITNIFQGYFRGLGKMKITL
                     NATLVQIVVRVSAAYLIASYFGVKGIALACLIGWIFMLGYQIPVLTKTWKKVKL"
BASE COUNT         1082 a          506 c          424 g          700 t
ORIGIN      
        1 aattttgacg atctcaacta tgatgtgaaa gcaaaaggaa cgcttgacat cagcaaaata
       61 tacaaagttt tctcccaaaa aggactcgat gtagatggtt caataaaagc cgatttagct
      121 ttgaaaggaa aacaaagcga tgccgaaaaa ggaaattata gcaaactgaa caacaaagga
      181 acattagaat taagaaacat tttaatcgct tcagaatacc tcccaaagaa attcctcatc
      241 aaagaaggca tcttcaaaat caatcaggac aaaatgactt tcaataattt cttggcagcc
      301 tatggacaat ctgattttag aatgaatggt tatttgcaaa atgtaatcaa ctttgctacc
      361 acgaatactg gagtattgcg aggttcattc aagctaaatt ccacctacat caatgccgat
      421 gaatttatgt cggaaactga agttgcttca acaaatacaa caaattcaga aacaaaacca
      481 gcagcaacaa aagtagaaac gggagtcatt ataattccat ctaacttgga tttaaaattt
      541 caagccaatg ccaataaaat caacttcaac gaattggcac ttaaagatgc taaaggaaat
      601 atgattgtaa aaaaaggcat cttgacaatg caaaatactg gcttcaattt gataggttgt
      661 acagtcaata tgaatgcaaa ataccaagca agcactccta aaaaagcaaa ttttgaatac
      721 gatataaaag cttctgattt tgacatcaaa aaggcataca acgaaataaa aatgttcaga
      781 gaaatggcaa gtgctgccga aaaagcatca ggaacagttt cgttagaata caaactcaaa
      841 ggaagattga atgctaatat ggaacccgtt tatccttcat tagttggaaa tggcgttgta
      901 tcagttaaag acattaaatt acacggaatg aaaatgttta gtgctgtagc caaaaaaacc
      961 aataacgacg gcattaaaaa tcctgaacta tctaaagttg aagtcaagag ttctattaaa
     1021 aacaacatca ttactttgga aagattcaag tttaaatttg caggatttcg accaagaatt
     1081 gaaggaacaa caagtttgga tggaaaatta aacatcaaaa tgagattggg tttacctcca
     1141 ttaggaatcc ttggaattcc gatggtaatc acaggaaata aggataatcc aaaagtaaaa
     1201 ataggtcgaa aatcagatga tttagaagaa acgaaagatt cggaaaagta attataactt
     1261 tacctttttc cacgttttag tcaaaactgg aatttgatag cctaacataa aaatccaacc
     1321 tatcaaacac gccaaggcga ttcctttgac cccaaaataa gaagcgatta aataagcggc
     1381 tgacacccta acaacaattt gcaccaaagt ggcattgagc gttattttca ttttgccaag
     1441 gcctcgaaag tacccttgaa atatattggt tattcctggt aataaataaa gaaaagccat
     1501 tagctgcaaa tactgaattc ctgcttcaac cacttcggat gctccatttc ccataaataa
     1561 aatcattatt tctttggcaa atacaaatac aataacggtc ataatcaaac tgtaaattac
     1621 ctcaagctta acaccaaccc aaaatccttt tcgaatacga ctaaaatttc cttttccttt
     1681 attctgtgcc aagaatccag taatcccgtg gctgatattt tgctgaacaa tcatcacaaa
     1741 gtcgtccact ctggtaacgg cattaaaagc tgcaatagca aaaactccta ggggattcac
     1801 tgcaccttga accagaagtt tacccaaatg caaagtgatt tgttgggtag cggttacaaa
     1861 actatatcct actataacac gtaacaaatc acgatccaaa ataaactcat tattcttaaa
     1921 tcgtaaaaaa gggactttaa atcgaacata caatacacag aaaagagccg ctgctgcttc
     1981 tgcaataacg gttgcccaag cagcgccttc gataccttgt tcccaaacgg caacaaaaag
     2041 taaatccaac acaatattaa gaactgcaga tgcacctaaa aaataaagag aaactttaga
     2101 attgcccaaa ctccgaagcg tagaggcgta aatattgtag atgaaagtaa aaacaagacc
     2161 aataaaaatg atttgcaaat aagacgtagc atcatcaata atttcagacg gcgtattcat
     2221 caaagtaagt attgaatgag aaaaaaagaa tcctaaaatc acgataaata gtgtaaagag
     2281 acctcccgaa attagagaag tagaaatcac ttttttcaag ctcgctaaat cgctattgcc
     2341 aaaaaagttg cccatcaata cagacattcc caaacaaatc ccaacaatca aaaagagcat
     2401 aatattcatc accggacttg ctgctcccaa ggcagccaaa gcgtgagcac ctataaaacg
     2461 tccaacaatt acagaatcag cggcattgta agtgagaaga aaaaaattac caataatcag
     2521 tggcaaggta aaatgaataa tctgaggagt aatcgctcct ctagtcaaat ctacaatatt
     2581 tttattattc attttgccta taaactaaat taaaatagtc aacaattata tcaataaatt
     2641 taatcctatt tttttatttt aaacttgttt tgcataaaaa aacaagaatt atttcaaaac
     2701 cattataaaa ca
//
LOCUS       DAOSNN010000291         2702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_37702_length_2702_cov_5.735550, whole genome shotgun sequence.
ACCESSION   DAOSNN010000291 DAOSNN010000000
VERSION     DAOSNN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37702_length_2702_cov_5.735550"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLQ70_11455"
     CDS             <1..506
                     /locus_tag="PLQ70_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQE35278.1"
                     /translation="LFALLDKAKYKVMDANLRAPYYTTEVLIELMSKADFIKLNDEEL
                     KKISIELKSPYNSFEQNMKFIAEKTNTKQICVTKGEFGAVLYYNDKFYYNSGYFIKVV
                     DTVGAGDSFLATLIVRLLKGKSPQKSLNYACAVGALVAGQEGANPKFSNTEIKKYMML
                     EEKKSKK"
     gene            complement(656..729)
                     /locus_tag="PLQ70_11460"
     tRNA            complement(656..729)
                     /locus_tag="PLQ70_11460"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(693..695),aa:Met,seq:cat)
     gene            884..1276
                     /locus_tag="PLQ70_11465"
     CDS             884..1276
                     /locus_tag="PLQ70_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HQE35279.1"
                     /translation="MDAKIRYEIEFPINSSPQLLYQYISTPSGLSEWFADNVNSRGEF
                     FTFIWDDSEEKARLCSKKSGEKVRFKWVDGNNKDSEYFFELNILVDEITKDVSLMVID
                     FAHKEEVDEARLLWENQISDLKHVIGSV"
     gene            1340..2179
                     /locus_tag="PLQ70_11470"
     CDS             1340..2179
                     /locus_tag="PLQ70_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQE35280.1"
                     /translation="MINFNGSIVSDDTNVLTHNRGFLYGDAVFETVKIVDSKILFLED
                     HYFRLMSSMRIVRMEIPMNFTMEYFEEQIISLAKAKNVADSARARITVYRNDGGYYLP
                     QTNTVSFLITTESIEEVPYSINKKEYIVDLYTDFYVAKQLLSSIKTTNKLINITASIY
                     ASENDLDNCLLLNDGKNVVDALQGNVFMLKGNKLVTPPVSDGCLNGVMRKQILSLAKT
                     IENIEVLEETISPFDLQKADELFITNVIKGIQPITKYRKKEFTKDFANQLLQKLNQTI
                     NRN"
     gene            complement(2176..2700)
                     /locus_tag="PLQ70_11475"
     CDS             complement(2176..2700)
                     /locus_tag="PLQ70_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQE35281.1"
                     /translation="MAEPSIIGDLSFNRSVILLADHDKKGSVGFIINKPLKYTINDLV
                     PDIKANFKIYNGGPVEQDNLYFIHNVPDLIPNSIEISNGIYWGGEYETTKELINNGQI
                     NKNNIRFFLGYTGWDEDQLEAEMAESSWIITKNNYENKIIGKSASHFWKKQIMELGGE
                     YLIWANAPENPTSN"
BASE COUNT          918 a          394 c          479 g          911 t
ORIGIN      
        1 ccttatttgc tttgttagat aaagcaaaat ataaagtgat ggacgcaaat ttaagggcac
       61 cttattacac gactgaagta ttgatagaat tgatgtcaaa ggccgatttt attaaactaa
      121 atgatgaaga attaaagaaa attagcatag agttaaagtc accctataat tcttttgagc
      181 agaatatgaa attcatagct gagaaaacaa acaccaagca aatttgcgtt accaagggcg
      241 aatttggagc cgttttatat tacaacgata agttttatta caatagcggt tattttataa
      301 aagtggttga tactgttggt gcaggagatt cttttttagc tactttaata gttaggttac
      361 tcaaaggaaa atctccccag aaatctttga attatgcttg cgctgttgga gcattagtag
      421 cgggtcagga aggagctaat cctaaatttt ctaatacaga aataaaaaaa tatatgatgc
      481 tggaagaaaa gaaaagcaaa aaataacttt ttattcttaa gattaaagcc gtctcaaaaa
      541 aacaattaga gacggctttt ttttagaatt caaatgataa gtattttttt aaattttaga
      601 cataaaaaaa cggattagag taaactaatc cgtgattttc aaaactttaa gtttgtagcg
      661 aggacgggag ttgaacccgt gacctcaggg ttatgaatcc tgcgctctaa ccaactgagc
      721 tacctcgcca tttctcaaga tgtaaatcat ctctcaatgc gggtgcaaat ataaaaatta
      781 aattagaggt tgcaagcata tatgtaataa aaaaaaatat ttttaaaaag gcttttttat
      841 tggctttata ttctataaat ttccaaaaaa ttaaatactg ttcatggacg caaaaatacg
      901 ttacgaaata gagtttccta taaactcatc tccacaatta ttgtatcaat atatatcaac
      961 tccatcaggt ttgtcagaat ggtttgctga caatgttaat tctcgtggcg agttctttac
     1021 ttttatttgg gatgattcag aagaaaaagc tcgactttgt tccaaaaaat ctggcgaaaa
     1081 agttagattc aagtgggttg atgggaataa taaagactca gaatactttt ttgaacttaa
     1141 tatattagta gatgaaatta caaaagatgt ttccttgatg gttatcgatt ttgctcacaa
     1201 agaagaagtt gatgaagcac gacttttgtg ggaaaatcaa atttccgatc ttaaacatgt
     1261 aattggatcg gtatagtaat tttgtttttt ttatgcctta tatttgtccc gatttaatcg
     1321 ggattttttt ttgaatacta tgataaattt taatggttct attgtgtctg atgacactaa
     1381 tgtgttgact cacaatcgcg gttttttata tggcgacgca gtttttgaaa cagtaaaaat
     1441 tgtagattct aaaattcttt ttttagaaga tcattatttt agattgatgt cctctatgcg
     1501 aattgttcga atggaaattc ctatgaattt taccatggaa tactttgaag aacaaattat
     1561 ttctttggcc aaagccaaaa atgtagctga ttctgcccga gctagaatta cagtttatag
     1621 aaatgatgga ggatattatt taccgcaaac caatacagtt tcttttttaa tcactacaga
     1681 aagcatagag gaagtgccct attcaataaa taaaaaagaa tacattgtag atttatatac
     1741 cgatttttat gtggctaagc agttgctttc gtcaataaaa accacaaata aattgataaa
     1801 catcacggca agtatttatg ctagtgaaaa tgatttggat aattgtttgc tacttaatga
     1861 tggtaaaaat gtggtcgatg ctttgcaggg aaatgtattt atgctcaaag ggaataaatt
     1921 ggtgacaccc ccagtttccg atggttgctt gaatggtgtt atgagaaaac aaattttgag
     1981 tttggccaaa actatagaaa acatagaagt acttgaagaa actatttctc cttttgatct
     2041 tcaaaaagct gatgaattgt ttattactaa tgtgattaaa ggaattcagc cgataacaaa
     2101 atatcgcaaa aaagagttta cgaaagattt tgcaaatcag cttttgcaaa aattaaatca
     2161 aactataaat agaaattaat ttgacgttgg gttttcgggt gcattagccc aaattaagta
     2221 ttctccgccc aattccataa tttgtttttt ccaaaagtgt gaagccgatt ttccaattat
     2281 tttgttttcg taattatttt tggtgataat ccaagaggat tcggccattt cggcttctaa
     2341 ttggtcttca tcccagccag tataacccaa aaagaagcgt atattatttt tgtttatttg
     2401 accattattg atgagttcct ttgtagtttc gtattcgcct ccccaataaa ttccatttga
     2461 gatttctata ctattcggaa ttaaatcagg aacattgtga atgaagtaaa ggttatcctg
     2521 ctcgacaggg ccaccattgt agattttgaa gtttgcctta atgtcaggca ccaaatcgtt
     2581 gatggtgtat tttaggggtt tgttgatgat aaaacccaca gatccttttt tgtcgtgatc
     2641 cgccaacaaa attactgacc tattgaaaga caaatctcca attatagaag gctctgcaat
     2701 aa
//
LOCUS       DAOSNN010000292         2689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_37931_length_2689_cov_5.372437, whole genome shotgun sequence.
ACCESSION   DAOSNN010000292 DAOSNN010000000
VERSION     DAOSNN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37931_length_2689_cov_5.372437"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLQ70_11480"
     CDS             <1..647
                     /locus_tag="PLQ70_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQE35282.1"
                     /translation="GLAALNGCPDKDGDGIADKDDACPDTAGLAAFQGCPDTDGDGLA
                     DKDDKCPTVAGPKSNGGCPILDTDKDGVNDKEDDCPTVAGPASNRGCPEVTPETIKQL
                     NAYGKTILFDSGKSSFKSQSYTVLQSIADILKEYPNSNFMIEGHTDSDGSNQLNQTLS
                     ENRAAAVKNYLIEKGISTDRLKSTGFGETKPLDTNKTAKGKANNRRVEVSLIKN"
     gene            713..>2689
                     /locus_tag="PLQ70_11485"
     CDS             713..>2689
                     /locus_tag="PLQ70_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQE35283.1"
                     /translation="MTDTSFLDKIAKVLIENYSGKLSNTIVVLPNKRAKIFLIEALKK
                     QVSTNILSPEIISIEEFIQDIASIRAIDPIELLFEFYEVYLSITEKPNQQSFELFANW
                     AKTLLQDFNEIDRYLLDPSHVLSYLKDIEDIKKWGIEVENKTQLLEKYIDFWKLLPNY
                     YQSLYNHLLNKGIGYQGLIYREAVNNLNHFSDSIKEKQFVFAGFNALNAAEERIIQHL
                     IASDQASIYWDVDQTFLNDPYHDAGLFVRRFKSSWKHYKSSPFEWIVDDFSQSKNILV
                     IGTPKSIGQAKITGSIIENIITENPKTTLDKVAVVLGEENLLVPLLYSLPSTVGALNI
                     TMGYSSKNNPAQILIAKLFKMHTNALSRNAKNYVLYYKDVLDILTHPLVEPFAKTNEL
                     VSVINQNNYTFITHQKLMELNPNQSDLFLLLFQKWENGSVQVLETISSLLQTIKTNLS
                     NDNEEEKITKAFVYAIFKTINKLINYYSQHSHIDKIETLYAIYKQVIDLAEVSFEGEP
                     LNGLQIMGVLESRVLDFETVIVTSMNEGKFPAGKSMNSFIPYDVKRELGLPTFKEKDA
                     IYTYHFYHLLQRAKNIYLIYNTESEGLDAGEKSRFITQLEVEKQQKHILTHEIYNAVL
                     PEIAYQPIKVPKSEAVMIRLKEIAEKGFSPSALTS"
BASE COUNT          914 a          445 c          496 g          834 t
ORIGIN      
        1 ttggtttagc tgcattaaac ggatgtcctg ataaagacgg agacggtatt gctgacaaag
       61 atgatgcttg tccagatact gctggtttag ctgctttcca aggttgtcct gatactgacg
      121 gagacggtct tgctgataaa gatgacaaat gtcctactgt agctggtcct aaatctaatg
      181 gtggttgtcc aattttggat actgacaaag atggtgttaa tgataaagag gatgactgtc
      241 ctactgtagc tggtccagct agcaatagag gatgtcctga agtaactcct gaaacgatta
      301 aacaattaaa tgcttatggt aaaacaattt tgtttgattc aggaaaatct tctttcaaat
      361 ctcaatctta cacagtatta cagtcaatag ctgatatctt aaaagaatat ccaaattcaa
      421 actttatgat tgaaggacat actgatagcg atggtagtaa tcaattaaac caaactttat
      481 ctgaaaatag agctgctgcg gttaaaaatt acttaattga aaaaggtatc tcaactgata
      541 gattaaaatc tactggtttt ggtgaaacta aaccacttga tactaataaa actgctaaag
      601 gaaaagcaaa taacagaaga gtagaagttt ctttgattaa aaactaaatt cttcttttaa
      661 ataatacagg aaaaagcctc gatttatcga ggcttttttt tatatttata aaatgacaga
      721 cacttctttt ttagataaaa tcgcaaaagt tttaattgag aactattcgg gaaaactctc
      781 caatacaatc gtggttttgc ccaacaagcg agccaaaatc tttttgattg aagctttaaa
      841 aaaacaagtt tcaactaata ttctatctcc agagattatt agcattgagg aatttattca
      901 ggatattgcg agtattcgtg ctatagatcc aatagagtta ttgttcgaat tttacgaagt
      961 ttatttgtct attaccgaaa aaccgaatca acaatccttt gaactttttg ccaattgggc
     1021 gaagacatta ctacaagatt tcaacgaaat tgatcgttat ttattagatc catctcacgt
     1081 tctttcgtac ttaaaagata ttgaagacat caagaaatgg ggaattgaag tggagaacaa
     1141 aacccaatta ctcgaaaaat acattgattt ttggaaatta ctacctaatt attatcaatc
     1201 actttacaat catttgttga ataaaggaat tggttatcaa ggattaattt atcgagaagc
     1261 ggtcaacaat ctcaaccatt tttcggattc cataaaagaa aaacaatttg ttttcgcagg
     1321 tttcaatgcc ttgaatgctg ccgaagaacg aattattcaa catttgatag cttccgatca
     1381 agcttctatt tattgggatg tcgatcaaac ttttttgaac gatccgtatc acgatgcagg
     1441 attgtttgtg cggcgtttca aatcgagttg gaaacactac aaatccagtc cttttgaatg
     1501 gattgtggac gatttttcgc aatcgaaaaa cattctggtt attggtacgc caaaatccat
     1561 tggtcaagcc aaaataactg gaagtatcat cgaaaatatc atcaccgaaa atccgaaaac
     1621 aacattagat aaagtggctg tggttcttgg agaagaaaat ttgttggtgc cgcttttata
     1681 ttcgttgcct tcaacggttg gtgctttgaa catcacgatg ggctattcaa gcaagaacaa
     1741 tccggcgcag attttaattg ctaaattatt caaaatgcac acgaatgcct tgtctcgaaa
     1801 tgccaaaaac tatgtcttgt attacaaaga cgttttggat attttgacgc atcctttggt
     1861 agagccattt gcaaaaacta acgaattggt tagtgttatt aatcaaaata attacacctt
     1921 cattacccat caaaaattga tggaattgaa tccaaatcag agtgatttgt ttcttttgtt
     1981 gtttcaaaaa tgggaaaatg gttcggtgca ggttttagaa accatttcca gtttgttgca
     2041 aaccataaaa acaaatttga gtaacgacaa tgaggaagaa aaaataacca aagcctttgt
     2101 ttacgcgatt ttcaagacca tcaacaaatt gataaattac tattcgcaac attcgcatat
     2161 cgataaaatc gaaaccttgt atgcgattta caaacaggtt attgatttgg ctgaagtttc
     2221 tttcgaagga gaacctttga atggtttaca aataatggga gtcttggaaa gtcgtgtttt
     2281 ggatttcgaa accgtgattg tcacttcaat gaacgaaggg aaatttccag caggaaaatc
     2341 aatgaattcc tttattccgt atgatgtgaa gagggaattg ggcttgccta ctttcaagga
     2401 gaaagatgct atttatacct atcattttta ccatttactc caacgtgcca aaaacattta
     2461 tctgatttac aatacggaaa gcgaaggttt ggatgccggc gaaaaaagtc gttttatcac
     2521 gcaattagaa gtcgaaaaac agcaaaaaca catactaact cacgaaatat ataatgcagt
     2581 tttgcccgag atagcttatc aaccaattaa agttccaaag tcggaagcgg tgatgattcg
     2641 tttgaaagaa attgcggaaa aaggattttc tccctcggcg ctgacgagt
//
LOCUS       DAOSNN010000293         2675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_38186_length_2675_cov_5.917939, whole genome shotgun sequence.
ACCESSION   DAOSNN010000293 DAOSNN010000000
VERSION     DAOSNN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38186_length_2675_cov_5.917939"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..470)
                     /locus_tag="PLQ70_11490"
     CDS             complement(123..470)
                     /locus_tag="PLQ70_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HQE35284.1"
                     /translation="MQDRLGDNVRLHHIVDAINEIENYIQDVDFDHFVKNSMMFNASL
                     RQLEIIGEASNRLSEMILNDNSSIPWARIIGLRNLVIHEYFGIDDITIWDVIKTNLPD
                     LKIKILDLLEDLG"
     gene            complement(460..753)
                     /locus_tag="PLQ70_11495"
     CDS             complement(460..753)
                     /locus_tag="PLQ70_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQE35285.1"
                     /translation="MSLTNSQINIIKDFFSKQPVLKAYLFGSYGRGSENKDSDIDLLV
                     ELDYSQPIGLEFIQMQLDLQKLLSKKIDLVSARGLSKYIQPILDKEKKLIYAR"
     gene            908..1819
                     /gene="cysK"
                     /locus_tag="PLQ70_11500"
     CDS             908..1819
                     /gene="cysK"
                     /locus_tag="PLQ70_11500"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016199894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQE35286.1"
                     /translation="MKAKSILETIGNTPVVQINKLFGNNKEVWIKLERSNPGNSIKDR
                     IALAMIEDAESKGLLNADSVIIEPTSGNTGIGLALVAAVKGYKVILVMPDSMSVERRK
                     LMEIYGAEFVLTPREKGMKGAIEKAAELVLETPNAWSPKQFDNPANVAVHERTTAQEI
                     IADFSNGLDYVITGVGTGGHITGVAKVLKAKFPNLKVIAVEPELSPVLSGGTPGPHPL
                     QGIGAGFVPSIYNSDLIDEVIQVSKDESFEFARKIAKEEGILVGISTGASLAAVAKKL
                     DAIPDGAVVLTFNYDTGERYLSIEGLF"
     gene            complement(1927..>2675)
                     /locus_tag="PLQ70_11505"
     CDS             complement(1927..>2675)
                     /locus_tag="PLQ70_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HQE35287.1"
                     /translation="TSNIADKKYGFFQSDKDFFQDVASELGMIPNKDGNDIGYERHPL
                     AYLVEAADDICYTIIDFEDGINLGLVPEDFALEYLIKLVKDSIDSSKYNTLTTKEDRI
                     SYLRALAIGSLINDAVKVFIENEEAILDGRFPFALTDKSKYKVQMDDIIKLSVKNIYQ
                     SREVIEKEIVGYQIIQTLLDKFITAFNNKFDGNSSNYDKLLLKMLPEKFLEEKADLYQ
                     RLLHICHYVSLLTDGNALELYDTINGRKKH"
BASE COUNT          896 a          459 c          458 g          862 t
ORIGIN      
        1 ccagaaaaca ttgaaaggtt agagaaacac cataaaaact ttcagaaacc tgcttataga
       61 aaagaaaact acatcaaagt agatgctaaa aaaaacatag aatattctaa tttgatttta
      121 gattagccca aatcttccaa caaatccaaa attttaattt ttaaatctgg tagatttgtt
      181 ttaataacat cccaaatcgt tatatcatct atgccaaaat attcgtgaat gacaaggttt
      241 cgtaacccaa taattcttgc ccaagggatg gaagaattat cgtttaaaat catttcagac
      301 agtcggttac tggcttctcc aattatttcg agttgacgta aactagcgtt aaacatcatc
      361 gaatttttta caaaatgatc aaaatccaca tcttgtatat agttttctat ttcatttata
      421 gcatctacga tgtgatgcaa gcgaacatta tctcctaatc tatcttgcat aaattagctt
      481 tttttcttta tctaaaatag gctgaatgta ttttgagagt cctctggccg aaactaaatc
      541 tattttttta gacagtaatt tttgtaaatc caattgcatt tgaatgaatt ctaaaccaat
      601 gggctggctg taatcaagtt ctaccaataa atcaatatca ctgtctttgt tttcacttcc
      661 tcttccgtaa gaaccaaaga gataggcttt caaaacgggt tgctttgaaa aaaaatcttt
      721 tatgatattt atttgagaat tagtaagact catttttttt taattggttt ttacaaattt
      781 aattaaaaat tcaaaacaaa ttacaaattg attttctcca ccttccaatc tgctctattt
      841 ttttccgtaa attagccatt gcttaaaaaa gcataaaata tactattcaa atcataacat
      901 aaaaaatatg aaagcaaaaa gcattctgga aaccatagga aacacgcccg tggttcaaat
      961 caacaagttg tttggaaaca acaaagaagt ttggattaaa ttagaaagat cgaatccagg
     1021 aaacagcatc aaagatagaa ttgctttagc aatgattgaa gatgcggaaa gtaaagggtt
     1081 actgaatgct gacagcgtca ttatcgaacc aacttctgga aatacaggaa taggtttagc
     1141 attggttgcg gcagtgaaag gatacaaagt gattctagta atgccagatt caatgagcgt
     1201 agaacgcaga aaattaatgg aaatctatgg agcagaattt gtgcttacac ctcgcgaaaa
     1261 aggaatgaaa ggcgctatcg aaaaagccgc cgaattagtc ctggaaacac caaatgcttg
     1321 gtcacccaaa caatttgaca atcctgccaa tgttgcagtt cacgaacgaa ctactgccca
     1381 agaaattatt gctgattttt ctaatggttt agattatgtc atcacaggag ttggaactgg
     1441 tggacacatt accggcgtgg ctaaagtctt gaaagccaaa tttcctaatc ttaaagtaat
     1501 tgccgttgaa cctgaattgt ctcccgtatt aagtggcgga actcctggtc cacatccatt
     1561 gcaaggtatt ggagccggtt ttgttccatc aatttacaac agtgatttaa ttgacgaagt
     1621 aattcaagta agcaaagatg agtcgtttga attcgccaga aaaattgcca aagaagaagg
     1681 aattcttgtg ggaatttcaa caggagcctc cttggcagca gtagcaaaaa aactagatgc
     1741 tattccagat ggagccgttg tattgacgtt taattatgat acaggagaac gctatttgtc
     1801 tatcgaagga ttgttttaaa atccatataa attatagaaa attaaacctt tggcaaagtt
     1861 ataaatccaa ctttgccaaa ggttttttag aatgattttg ttcttggttt gcctcgaact
     1921 tcgtatttaa tgttttttcc taccattgat ggtatcgtat aattccaaag catttccgtc
     1981 ggtcaaaagg gaaacataat ggcaaatatg aaggagtctt tggtataaat ccgctttttc
     2041 ttctaagaat ttttcgggta acattttcaa caaaagttta tcgtaattag acgaattacc
     2101 atcaaattta ttattgaatg cagttatgaa tttatccaac aaggtttgga taatttggta
     2161 acccacaatt tctttttcga taacctcgcg gctttgataa atgtttttga cactcaattt
     2221 gatgatgtcg tccatctgta ctttgtattt gcttttgtcg gttaaagcaa aaggaaatct
     2281 tccgtccaaa attgcttctt cattttcgat aaaaactttt acagcatcgt taatcaaact
     2341 gcctattgcc agagctctca aataactgat tcggtcttct ttggtcgtta aagtattgta
     2401 tttggaagag tcaatactgt ctttaactaa ttttatcaaa tattctaagg caaaatcttc
     2461 gggaactaaa cctaaattga tgccatcttc aaaatcaatg atggtgtagc aaatgtcatc
     2521 ggcagcttcg accaaataag ccaaaggatg tctttcatag ccaatgtcgt taccgtcttt
     2581 gtttggaatc attcccaatt cagaagctac gtcttgaaaa aaatctttgt ctgactggaa
     2641 gaaaccgtat tttttgtcgg caatgttgga ggttg
//
LOCUS       DAOSNN010000294         2666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_38345_length_2666_cov_4.608962, whole genome shotgun sequence.
ACCESSION   DAOSNN010000294 DAOSNN010000000
VERSION     DAOSNN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38345_length_2666_cov_4.608962"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PLQ70_11510"
     CDS             <1..1191
                     /locus_tag="PLQ70_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HQE35288.1"
                     /translation="PDFYASQRSTFDPISGTKDQTLFQSFRYGIDKLRYEFPAPDGEY
                     LVELFFTEPWYGTGGGLDCKGWRLFDVAINDNVVLKDLDIWSEVGQNTALKKTFVVKS
                     VNGKITISFPNIKAGEAIISAIAIATKDKKVKGASASPRNIQNVTSSSETKIGSWLDI
                     NSKQYPDSDITFTKLPSELFGADYLQIPSNSKVSGSFVTKEDSEVYLFSTSKDSISGY
                     KKMDETAKNSDRVEFSVFHKKVKKGEKIDFESCTVAVVPTYDMGEKDDSRPVVLLEAE
                     TAKTTGTGIEKGNFKKADYIEFKEKTKNSIQFEVKPGVAGIYLMRFRFMNKNETPVKV
                     KFKMEDAYGILMRNDEIEFSTASEKWKVLSTTSGGYINAGTYKITLESEDIKGLLLDS
                     FEFQ"
     gene            1204..>2666
                     /locus_tag="PLQ70_11515"
     CDS             1204..>2666
                     /locus_tag="PLQ70_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQE35289.1"
                     /translation="MKSHKNVLQKIGIVLTILLSTACFAQSKKVRIVEDFNKNWNFKL
                     GDYPKAINANFSTADWRSLQLPHDWSIEGAFNKDNPTKQAQGFLPAGKGWYRKVFTVP
                     ANWKNKTVSVEFDGVFKNSEVFINGHSLGIRPNGYISFSYELSQYLNFGKQNIIAVKV
                     DNDAQPNSRWYTGSGIYRNVRLVASEKLHVAKWGTYVTTPEVSEAKASVHLEVTVENK
                     TNVEKEFQLVTSILDANNKEVAKASTTEKIGANANAKKVHNLNVNQPKLWDTENPNLY
                     KIVTSIYEKSKLIDNYETPLGFRFFSFDAEKGFSLNGKPTKILGVCLHHDNGALGAVE
                     NIHAVRRKLKLLKEMGCNAIRMSHNPHSLEMMQLCDEMGFIVQDEAFDVWKKKKVTND
                     YHKDWDAWHRQDLEDFIKRDRNHPSVIMWSIGNEIREQFDSTGIAITRELAKIVKSLD
                     TTRPVTSALTENVIEKNFIYQSGALDLLGFNYKHEDY"
BASE COUNT          931 a          467 c          565 g          703 t
ORIGIN      
        1 cctgattttt atgcgagcca aagaagcact ttcgacccaa tttcaggaac aaaagaccaa
       61 actttatttc aaagtttcag atacggaatt gacaaactgc gttacgaatt ccctgctccg
      121 gacggagaat atctggtcga attgtttttc acggaacctt ggtacggaac cggtggcgga
      181 ttggattgca aaggctggcg tttgtttgac gttgccatca atgacaatgt cgttttgaaa
      241 gatttggata tttggagtga agtaggtcaa aatacagctc taaagaaaac ctttgttgtg
      301 aaaagtgtaa acggaaaaat taccatttct ttcccaaata taaaagcggg tgaagccata
      361 atttcggcaa ttgccatcgc aactaaagat aaaaaagtaa aaggagcttc ggcttcgcca
      421 agaaatattc agaatgttac aagtagttcc gaaactaaaa ttggttcttg gctggatatt
      481 aattccaaac aatatccaga ttcagacatt acttttacaa aattgccatc tgaattattt
      541 ggtgccgatt atcttcaaat tccttcaaat tcaaaggttt ctggttcatt tgtaaccaag
      601 gaagattcgg aagtttatct gtttagtaca tcaaaagatt ctatttccgg ttacaaaaaa
      661 atggatgaaa cagctaaaaa ctcagataga gtagaatttt cggtttttca taaaaaagta
      721 aaaaaaggag aaaaaataga ttttgaaagt tgtactgtcg cagtcgttcc aacctacgat
      781 atgggcgaaa aagacgattc acgacctgtt gtgcttttgg aagcagaaac agccaaaacc
      841 accggaaccg gaatcgaaaa aggaaatttc aaaaaagccg attacatcga attcaaggaa
      901 aaaacaaaaa acagcatcca gtttgaggtg aaaccgggcg ttgcaggaat ctatctgatg
      961 cgcttccgtt ttatgaacaa aaacgaaacg cctgtaaaag tcaaattcaa aatggaagat
     1021 gcctacggaa ttttgatgcg aaatgatgaa attgaatttt ccaccgcatc agaaaaatgg
     1081 aaagtattaa gtaccacttc gggtggttat atcaacgcag gaacctataa aattacttta
     1141 gaatcagagg atataaaagg cttgcttttg gattcttttg agtttcaata aaaaaataaa
     1201 attatgaagt cacataaaaa cgttttgcaa aaaattggaa tagtcttgac aatattgctt
     1261 tcgacggcct gtttcgcaca atccaaaaaa gtccgaatag ttgaagattt caataaaaac
     1321 tggaacttca aactaggaga ttatccaaaa gctatcaatg cgaatttcag cactgccgat
     1381 tggcgttcat tacaattgcc tcacgattgg agtatcgaag gggcattcaa taaagacaat
     1441 ccaaccaaac aggcacaagg atttttgccg gcaggaaaag gctggtatcg caaggttttt
     1501 actgttcctg cgaattggaa aaacaaaacc gtttcggttg aatttgacgg tgttttcaag
     1561 aacagcgaag tattcataaa cggtcattcg ttgggaattc gcccgaacgg atatatttca
     1621 tttagttacg aattgtcaca atatttgaat ttcggaaaac agaatatcat cgcggtaaaa
     1681 gtcgataacg atgcgcaacc caattctagg tggtacaccg gttcgggaat ttacagaaat
     1741 gtgcgattgg tggccagcga gaaactacac gtagctaaat ggggaactta tgtgaccacg
     1801 cctgaagttt cggaggcaaa agcatctgta catttggagg tgactgttga aaataaaaca
     1861 aatgtcgaaa aggaatttca attggtgacg tctattttgg atgccaataa taaagaagtg
     1921 gccaaagctt ctacaactga aaaaattggt gccaatgcca atgcaaaaaa ggttcacaat
     1981 ctgaacgtaa atcagccgaa attgtgggat acagagaatc caaatctgta caaaatagtt
     2041 actagcattt atgaaaaatc aaaattgatt gacaattatg aaactccgct tggtttccgt
     2101 tttttcagtt tcgatgccga aaaaggattt tcattaaacg gaaaaccaac caaaatatta
     2161 ggggtttgcc tgcatcacga caacggtgcc ttgggagcgg tagaaaatat tcacgccgtt
     2221 cgaagaaaac tgaagttgct gaaagaaatg ggttgcaacg ccattcgaat gtcgcataat
     2281 ccacattcgt tggaaatgat gcaactgtgt gacgaaatgg gatttattgt tcaggacgaa
     2341 gcttttgatg tttggaaaaa gaaaaaagta accaacgatt accacaaaga ttgggatgcg
     2401 tggcacagac aggatttgga agatttcatc aaaagagacc gcaaccatcc atcggtaata
     2461 atgtggagta tcgggaacga aatccgtgaa caatttgaca gcaccggaat tgcgattaca
     2521 agggaattgg ccaaaatcgt aaaatcgctg gacacgaccc gcccggtaac ttcggctttg
     2581 acagagaatg taatcgagaa aaatttcatt taccagtcgg gtgctttgga tttgttgggg
     2641 ttcaattaca aacacgaaga ctacaa
//
LOCUS       DAOSNN010000295         2655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_38554_length_2655_cov_6.494231, whole genome shotgun sequence.
ACCESSION   DAOSNN010000295 DAOSNN010000000
VERSION     DAOSNN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38554_length_2655_cov_6.494231"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1294)
                     /locus_tag="PLQ70_11520"
     CDS             complement(20..1294)
                     /locus_tag="PLQ70_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HQE35290.1"
                     /translation="MYALVDCNNFYASCERVFQPKFNGKPVAILSNNDGCVISRSNEA
                     KAVGVPMGAPAFQIKELVKEKNVQLFSSNYPLYGDLSNRVMAILNQFTPNVEIYSIDE
                     AFLNFDGLDISNYHDYGLQMKTRVQKWVGIPVCIGFAKTKALSKVANKIAKKFQERTQ
                     GIYVIDTEEKRIKALKWTKIEDVWGIGYRMNKKMKSKNIKTALDFIQPQHEAWIRKEM
                     GVVGMRLKYELEGKSVLDLEPIPNQKKSIAVTRSFPKQIADFDLLRERVATFASVCAE
                     KLRKQKSCCHTIIVMLVIDKHSIQTNKYYFNMAVTLPFATNSTLTISNTAIAILKKLH
                     TGNENIKFKKAGVIVTELIDENKKQLQLFDEENPKHLALMQAIDKLNHKIGDKKVKLA
                     TQNLNLTWNMNQNHLSPKYTTNFNDILEIRCP"
     gene            complement(1504..2208)
                     /locus_tag="PLQ70_11525"
     CDS             complement(1504..2208)
                     /locus_tag="PLQ70_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HQE35291.1"
                     /translation="MSVQIWSDIMCPFCYIGKRRIEEALSQFEHKDAVAIEWKSFQLD
                     AGFIPSSDDNLAEHLADKYGRDIDWAQTMLENTTQNAKTAGLDFHFEKSVMANSHNAH
                     RLLHLAKKYQLANELKELLFKAYFTDGKDLNNLETLTELGIAVGLEAEIVAQVLHSNA
                     YSQEVKQDIQQAGAIGVQGVPFFVFDNKYAISGAQPTTTFLQTLEKVWQEGQFDSKIT
                     LVNSTTENSCDINGCE"
BASE COUNT          756 a          467 c          500 g          932 t
ORIGIN      
        1 gttagctttt ggtctttttt tagggacatc gtatttcgag aatatcgttg aaattagtag
       61 tgtattttgg cgacagatgg ttctggttca tattccaagt caggttcaag ttttgggtgg
      121 cgagttttac ttttttatca ccaattttat gattgagttt gtctattgcc tgcattaaag
      181 ccaaatgttt tgggttttct tcatcgaaca attgcagttg ttttttgttt tcgtcaatca
      241 attcggttac gattacgccc gctttcttga acttgatgtt ttcatttccg gtgtgcaatt
      301 ttttcaatat cgcaatggct gtattcgaaa tagtcaaggt cgaattagtt gcaaaaggca
      361 gtgtaacagc catattaaaa taatatttat tcgtttgaat gctgtgtttg tcaatgacca
      421 acattacaat aattgtgtgg caacaggatt tttgttttcg taatttttca gcacaaaccg
      481 aggcaaaagt cgccacacgt tcgcgcaaca aatcgaaatc ggcaatttgt ttgggaaaac
      541 ttctagtaac ggcaatactt ttcttttggt taggaatggg ttccaaatcc aaaaccgatt
      601 ttccttccaa ttcatatttc aagcgcattc ctacaacgcc catttctttt ctaatccaag
      661 cttcgtgttg tggttggatg aagtctagcg cagttttaat gtttttcgac ttcatttttt
      721 tattcattcg atagccaata ccccaaacat cttcaatttt tgtccatttt agggctttaa
      781 tgcgtttttc ttcggtgtca attacataaa tgccttgggt tctttcctga aatttcttgg
      841 cgattttgtt ggctactttc gataaagctt tggttttggc aaagccaatg caaaccggaa
      901 ttccgaccca tttttgtaca cgagttttca tttgaagacc gtaatcgtgg taatttgaaa
      961 tgtctaatcc atcaaaattt aggaaagctt cgtcaatgct atagatttct acattgggtg
     1021 taaattgatt taaaatcgcc attactcgat tgctcaaatc gccatagagt ggataattgg
     1081 aagaaaacaa ttggacgttt ttttctttga ccaattcctt gatttgaaat gctggagcgc
     1141 ccattggaac gccaacggct ttggcctcat tgcttctaga aatcacgcag ccgtcgttgt
     1201 tggataatat tgcaacaggt tttccgttga atttcggttg aaaaacacgt tcgcaggaag
     1261 cgtagaaatt gttgcaatcg actaaagcat acatcctgta aaattacaaa acagcagttt
     1321 atatcgtggc ggaacaaaag ttaattttag cgattgttga taaaaaaaat gggacgcaga
     1381 tgagacggat tcgctttgcg aaaacacgga taaaaacgga tttttacttt tatattattt
     1441 gaaaaattcg tttttatccg cgtttgcctt ggcatcggag tcatctgtgt cctaataata
     1501 ttcttattca catccattaa tatcgcaact gttttccgta gtgctgttta ccaaagtaat
     1561 tttcgaatca aattgtcctt cttgccatac tttttccaac gtttgtaaaa aagtggtggt
     1621 tggctgtgcc cccgaaatag catatttgtt gtcaaaaaca aaaaaaggaa ctccttgaac
     1681 acctatcgct ccagcttgtt gaatgtcttg ttttacttct tggctataag cgttagaatg
     1741 taaaacttga gcaacaattt cggcttcgag tcctacggcg attcctaatt cggttagtgt
     1801 ttcgagattg tttaaatctt tgccatcggt gaaataagct ttaaaaagca gttctttcaa
     1861 ttcgttagca agttgatatt ttttagccaa atgcaataat cggtgtgcat tgtgggaatt
     1921 cgccatcact gatttttcaa aatgaaaatc caatcctgcc gtttttgcgt tttgcgttgt
     1981 attttctaac atcgtttgag cccaatcgat atctcttccg tatttatctg ccaagtgttc
     2041 ggctaaatta tcatcggatg aaggaataaa acctgcatcc aattgaaaac ttttccattc
     2101 gatggcaacg gcatctttgt gttcgaattg gcttaaagct tcttcgattc ttctttttcc
     2161 tatataacaa aacggacaca taatatccga ccaaatctgt acgctcaatt tattttccat
     2221 atcaagattg ccaattgatt ttctgttgca aaattaattc attctggttg gaactgtttt
     2281 tactttaagt ttttattagg atggaatgga aaataatctc gaagattagt acgatttttc
     2341 tagccactgt cattacaata ggcaataaag acaaggtttt tttgtagtaa aaatgagtat
     2401 atttgaagga tcaaacgcat tgcaatagtc attttgaaaa agttggaacg gtatgtagga
     2461 atgggtgtga agtcatcgac atttgcacag catccaagtt ctgatgatgt ttagttttaa
     2521 aagaagactt cgggggagtg gatagaaaaa aaccaaatgc aattagtgac aaaattctta
     2581 gagaaagcgt aaaataataa tttgtcttgt cctgaaataa cgatacacaa aattcatcgt
     2641 tatggaaaac gccca
//
LOCUS       DAOSNN010000296         2654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_38570_length_2654_cov_4.212389, whole genome shotgun sequence.
ACCESSION   DAOSNN010000296 DAOSNN010000000
VERSION     DAOSNN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38570_length_2654_cov_4.212389"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLQ70_11530"
     CDS             <1..464
                     /locus_tag="PLQ70_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013307595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQE35292.1"
                     /translation="YGNQEPEVFHQSRLQLNTTFGAINTIFLLTSGLFMAAAVHEFKE
                     NNVQKSSFYFKLAMLGGLLFIVLKSIEYYHKIEAGISLETNMFFTFYWLLTAFHLIHV
                     LVGLVILAWTNKGMTKKNSKTTLEDVEACAAFWHMCDLIWLLLFPVLYLIF"
     gene            467..709
                     /locus_tag="PLQ70_11535"
     CDS             467..709
                     /locus_tag="PLQ70_11535"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HQE35293.1"
                     /translation="MKKSLIISYGILLILTLSTALIASSGAISKGIAFLILGISVIKF
                     LLVAFQFMELKKANSFWKISLIGTLGLLLILIIGLK"
     gene            826..2316
                     /gene="nirK"
                     /locus_tag="PLQ70_11540"
     CDS             826..2316
                     /gene="nirK"
                     /locus_tag="PLQ70_11540"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HQE35294.1"
                     /translation="MKKPFKFSNPCKVIAFTLFATVSFLSCDKKEVKHNAEYYENIQV
                     EGQKEAELTAPPFVPKPIGDRAATKLVVNMEIKEVEGEMVDGVKYNYWTFGGSVPGSF
                     IRTRVGDEVEFHLKNHPDNKMPHNIDLHAVTGPGGGATSSLVAPGHEKVFNFKTLNPG
                     LYVYHCATAPVGMHIANGMYGLILVEPEGGLPPVDKEYYIMQGDFYTKGSYGEQGSQP
                     FDMNKAIKEQPDYVVFNGKVGAVAGDNAITAKVGETVRLYMGNGGPNLVSSFHVIGEI
                     FDKVHIEGGDMINENVQTTLIPSGGSAIVEFKVDVPGTFILVDHSIFRAFNKGALGML
                     KVEGVENKKIYSGTTQEGIYLPEGGTIQNMPKENGAAKSTTAKTVPEQIKSGKELFGR
                     TCFACHQPEGQGVPNKFPPLEKSDFLNANPDRAIGAVLHGLSGEITVNGKKFNNVMTS
                     QNLNDEEVADVLTYVYNSWGNNKTVITPAKVKTIREKPVSKPVSTH"
     gene            2426..2654
                     /locus_tag="PLQ70_11545"
                     /pseudo
     CDS             2426..2654
                     /locus_tag="PLQ70_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016419727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
BASE COUNT          916 a          417 c          535 g          786 t
ORIGIN      
        1 attacgggaa tcaagaacct gaagtatttc atcaatctag gttgcaactc aatacaactt
       61 ttggagcgat aaacaccatt tttttattga ccagcggttt gtttatggcg gctgcggttc
      121 acgaattcaa ggaaaataat gttcagaaat cgtctttcta tttcaaattg gcgatgttgg
      181 gtggattgtt atttattgtt ttaaaaagca ttgaatacta ccataaaatt gaagctggaa
      241 tctcattgga aaccaatatg ttttttactt tttattggtt actaactgca tttcacctga
      301 ttcatgtttt ggtgggattg gtgattttgg cttggactaa caaaggaatg actaagaaaa
      361 attcaaaaac aacccttgaa gacgttgagg cttgcgcggc attttggcat atgtgtgact
      421 tgatttggct actgctgttc cccgttttat atttaatttt ctaacaatga aaaaatcatt
      481 aatcataagt tacggcatct tgttgattct tacattatcg acggcattaa tagcttcttc
      541 gggtgcaatt tctaaaggga tagcttttct aatattgggg atttcggtta tcaaattttt
      601 gctggttgcg ttccaattta tggaattgaa aaaggcaaat tctttttgga aaattagttt
      661 aattggcact ttaggattac ttttaatttt gattattggt ttaaaatgag aatcttaagg
      721 aaaatttcat aaagtaaaat ttattttttt agctcaaatt ttaatttaat cagacaataa
      781 aagatattaa caaaccatta tataaaatcc tttaaactaa aaattatgaa aaaaccattt
      841 aaatttagta atccctgcaa agtaatcgct tttactttat ttgctaccgt aagctttttg
      901 tcttgcgata aaaaagaagt gaagcataac gcagaatatt acgaaaacat acaggtcgaa
      961 ggtcaaaaag aggccgaatt gactgctcca ccatttgtgc caaaaccaat aggagatcga
     1021 gccgcgacaa aattagtcgt aaatatggaa atcaaagaag tggaaggcga aatggttgat
     1081 ggagtaaaat acaactattg gacatttgga ggttcagtac caggtagttt tatcagaact
     1141 agagtaggag atgaggtaga atttcatttg aaaaatcatc ctgacaacaa aatgccacac
     1201 aatatagatt tgcatgcagt tactggaccg ggcggaggag caacatcctc tttggtcgct
     1261 cctggacatg aaaaggtctt taattttaaa actttaaacc cggggttgta cgtctatcat
     1321 tgcgctacag cacctgttgg aatgcacatt gccaatggaa tgtatggttt aatattggta
     1381 gagccagaag gaggattacc tccagtggac aaagaatatt acattatgca aggtgatttt
     1441 tataccaaag gaagttacgg agagcaaggt tctcaaccgt ttgatatgaa taaagccata
     1501 aaagagcaac cagattatgt ggttttcaat gggaaagttg gagcggttgc aggagacaac
     1561 gccataactg ctaaagtagg agaaacagtt cgtttgtata tgggtaacgg aggtcccaat
     1621 ttggtttctt cgttccacgt aattggtgaa atttttgaca aagttcatat tgaaggcgga
     1681 gatatgatta acgaaaatgt tcaaaccact ttgattccct caggaggttc tgcaattgtt
     1741 gagttcaaag ttgatgttcc aggaactttc attttggtcg atcactctat ttttagagca
     1801 ttcaataaag gagctttggg aatgcttaaa gtagaaggag tagagaataa aaaaatatat
     1861 tcaggaacca cacaggaagg aatttatttg cctgaaggag gaacaattca aaacatgcca
     1921 aaagaaaatg gtgctgccaa aagtactact gccaaaacag ttcctgaaca aatcaaatca
     1981 gggaaagaat tgtttggaag aacttgtttt gcttgtcacc aacccgaagg acagggagtt
     2041 cctaataagt ttccaccatt ggaaaaatct gatttcttga atgcaaatcc agatagagcc
     2101 attggggctg tattacacgg attgagtggc gaaattaccg tgaatggtaa gaaattcaac
     2161 aacgtaatga ccagccaaaa cttgaatgat gaagaagtag ccgatgtttt gacctatgta
     2221 tataacagtt ggggcaataa taaaactgtt attacgccag caaaagtaaa aacaattaga
     2281 gaaaaaccag tatcaaaacc agtatctaca cattaatata aaaaccagtt ataaatcaat
     2341 aaattaaaat taagaatcaa tgaaaaagta tgttttaagt atcgtaatga tgggattagg
     2401 tttgtatagc tacagccaaa atacagccaa aggaaaagcc gtttttgcaa aaacgtgtat
     2461 cgcttgtcac caagcaacgg gagcaggaat accaggagct tttccgccat tggcaaaatc
     2521 cgattatctg aatgcggatg taaatcgagc cattaaacaa gtaatcaaag gatctaatgc
     2581 gccaattact gtaaatggga aaaaatatac aacacctatg cctgctcaac ctttgaatga
     2641 tcaagaaatc gctg
//
LOCUS       DAOSNN010000297         2650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_38656_length_2650_cov_3.581118, whole genome shotgun sequence.
ACCESSION   DAOSNN010000297 DAOSNN010000000
VERSION     DAOSNN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38656_length_2650_cov_3.581118"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLQ70_11550"
     CDS             complement(<1..443)
                     /locus_tag="PLQ70_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HQE35295.1"
                     /translation="MITYYKNNKGLTQTENREENCWINVVCPTKEESKFLLKELQVPE
                     DFYNDIEDIDERPRIEFENGWTLIILRIPFKSEDPKLPFNTAPLGLMFKDDVFLSLCF
                     HQTEILKDFVLYTKRKKIIIENDFDLVLKLLLSSSVWFLKYLKQI"
     gene            complement(742..1107)
                     /locus_tag="PLQ70_11555"
     CDS             complement(742..1107)
                     /locus_tag="PLQ70_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35296.1"
                     /translation="MKNLIITLFFLASIPCFSQDTITTAKVQEYMDKEVCVVGKVVSF
                     KLASEGKFTNYINIDKPYPESVFTVVITNNYLEKLNIKIEDLKDKTIFIKGKITTYKN
                     DPKQIPQIYNPTSIVVKKE"
     gene            complement(1124..2227)
                     /locus_tag="PLQ70_11560"
     CDS             complement(1124..2227)
                     /locus_tag="PLQ70_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQE35297.1"
                     /translation="MKIKNIIVVLLVFCSLFGANAQQKKYAVRTVAFYNFENLFDTIN
                     NPNNDDEWTPKGLQRWTSEKYKQKLENLSKVLMQIGTNDQQKEAPTLIGGSEIENRGV
                     LEDLVKQPNLIQEDYGIVHFDSPDRRGIDVALLYKKKYFKPTSFINIPLIIYRGNNNE
                     KEKETEEDKVDKDKIEVTNDNRVYTRDILLVTGFLDGEEVNVLVNHWPSRSGGEKKSS
                     PFREAAGRLAKKTMDSIYKVNPKAKIILMGDLNDGSYNKSVKVGVGAKLKKSEVQPFG
                     IFNPFEQMYKDGNATLFYRDSGDIFDQIMVSETLIKPDFSTYQYWKAGIYNKPFMIEK
                     FGRYAGYPLRHSENEIGYSDHFPVYIYLVKEVK"
     gene            2361..>2650
                     /locus_tag="PLQ70_11565"
     CDS             2361..>2650
                     /locus_tag="PLQ70_11565"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HQE35298.1"
                     /translation="MKKIIFSALFVMQIVFAFGQTGTGVTGKVIDFKTQKPLQSVIVS
                     IQNTNLTQLTDSNGKFTFDKVEVGNQLLRIRTNGYKEQLLQIEITEGKLLDL"
BASE COUNT          812 a          411 c          438 g          989 t
ORIGIN      
        1 ttgatttgtt taaggtattt caagaaccaa acactggagg ataacaacaa ttttagcact
       61 aaatcgaaat cattttcgat aataattttc ttgcgtttag tgtataaaac aaagtctttg
      121 agaatttctg tttgatgaaa acaaagagaa agaaacacat cgtctttgaa cattaatccc
      181 aatggtgctg tattgaaagg cagttttgga tcctcacttt tgaaaggaat tctaaggata
      241 ataagtgtcc aaccgttctc gaattcgata cgcggacgtt catcaatatc ttcgatgtca
      301 ttataaaaat cttcgggaac ttggagttct ttgagcagga atttggattc ttcttttgtg
      361 ggacaaacta cattaatcca gcagttttct tcacggtttt cggtttgagt taaccctttg
      421 ttgtttttgt aataggtaat cataatgcaa tgcgatgttt taaggaatat cgcttacatt
      481 aggcaacgat tattcctttt tgtgatttgt ttccttcgaa taataatcgt ccatggtgga
      541 ggctaaaatt taatttttgg caaaaatagg gttttaaatt gctataaccc ttaattcttt
      601 cttaatttaa atgctaaagt agttttttgc tatttgagcg aggtgttttt gaaaccatta
      661 agaccgccaa aaaaatcact ttggacgatt tctttgaaac tatcttaatg gtttgaaaat
      721 tgttttttta aaagtaaaaa attactcttt ttttaccaca atactcgtgg gattgtaaat
      781 ttgtggtatt tgttttgggt catttttata ggtggtaatt ttacctttta tgaaaatggt
      841 tttatctttc aaatcttcga ttttgatgtt tagtttttcg agataattgt tggtgattac
      901 taccgtaaaa acgctttcgg gataaggttt atcgatgttg atgtaattgg taaatttgcc
      961 ttcggaggcg agcttgaagg agactacctt tcccactaca caaacttctt tgtccatata
     1021 ttcttgaact ttagccgttg tgatggtgtc ttgcgaaaaa caaggtattg atgctagaaa
     1081 aaataatgtt attattagat ttttcattgt aatgaatttg ttattatttt acctctttga
     1141 ctaaataaat ataaactgga aagtggtcgc tgtatcctat ttcgttttct gaatgtcgca
     1201 aaggatagcc agcatatcgt ccgaattttt cgatcataaa cggcttattg taaattcctg
     1261 ctttccaata ttgataagta gaaaaatcgg gttttattaa ggtttccgaa accataattt
     1321 ggtcaaaaat atctcctgaa tctctataaa ataaagtggc atttccgtct ttatacattt
     1381 gctcaaaagg attgaaaatg ccaaatggtt gtacttcaga tttttttagt ttggctccaa
     1441 ctccaacttt cacactttta ttatagcttc cgtcattcaa atcacccatc aaaatgattt
     1501 tggcttttgg gttcacttta taaatagaat ccatcgtttt ttttgctaat cttcctgcag
     1561 cttcgcgaaa gggactgctt tttttctccc ctcctgagcg tgatggccag tgattgacca
     1621 atacatttac ttcttcacca tcaagaaaac cagttactaa cagaatatct ctagtataaa
     1681 ctctattgtc attagtcacc tctattttat ctttatcgac tttatcttct tcggtttctt
     1741 tttccttttc gttattgttt cctctgtaaa ttattaaagg aatgttgatg aagctcgtgg
     1801 gtttgaaata ctttttttta tacaaaagag ctacatcaat acctcttctg tctggggaat
     1861 caaaatgtac gattccgtaa tcttcttgta ttagattagg ctgttttact aagtcttcaa
     1921 gaacgccacg gttttctatt tcggaacctc caatcaaggt aggtgcttct ttttgttggt
     1981 catttgtgcc aatttgcatc aagactttcg aaagattttc taatttttgc ttgtattttt
     2041 ctgaagtcca tcgctgaagt ccttttggag tccattcatc atcattgtta ggattgttta
     2101 tggtgtcaaa taaattttcg aaattataaa aggctacagt tcggacagca tatttttttt
     2161 gttgcgcatt ggctccaaac aaagaacaga aaactaacaa aacaacaatg atatttttga
     2221 ttttcatgat attatattat tatagtgtga aatttaacaa aaacttaagc gcaaaagtag
     2281 ataataaaaa ttataaatgt agctttgtat tcgttaagaa ttgataattc ttatgattat
     2341 gaattaactt taattataac atgaaaaaaa ttatttttag tgcactattt gtgatgcaaa
     2401 tagtgtttgc ttttggacaa actggtactg gagttactgg taaagtaatc gattttaaaa
     2461 ctcagaaacc attacaaagt gttattgtat ctattcaaaa cacaaatcta acacaactta
     2521 ctgactcaaa cggaaaattt acatttgaca aagtagaagt tggaaatcaa ttattacgca
     2581 ttagaacaaa cggctacaaa gagcaattac ttcaaataga aattacagaa ggaaaactat
     2641 tggatttaga
//
LOCUS       DAOSNN010000298         2631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_38996_length_2631_cov_4.690606, whole genome shotgun sequence.
ACCESSION   DAOSNN010000298 DAOSNN010000000
VERSION     DAOSNN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38996_length_2631_cov_4.690606"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLQ70_11570"
     CDS             complement(<1..622)
                     /locus_tag="PLQ70_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQE35299.1"
                     /translation="MNVVAIFDIGKTNKKVFLFNENYQIVWEKSVILAETVDEDGFPC
                     ENIVELSQWVADRLTELKELKEYVLKAINFSTYGASFVYLDEQGKSLTPLYNYLKPYP
                     ETLLENFYPKYKGKKKFAVKTASPILGSLNSGMQIYRLKEEKPELFAKVKYCLHLPQY
                     LSSVLTHHYFTDITSIGCHTGLWNFKKMKYHKWVTNEGIIDKLPPIQ"
     gene            complement(760..2034)
                     /locus_tag="PLQ70_11575"
     CDS             complement(760..2034)
                     /locus_tag="PLQ70_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HQE35300.1"
                     /translation="MLIDSNKIASHNEGLLKSHQNKLVFTASEIAETENIIQKLIDFQ
                     IAIPSWALGTGGTRFGRFPGGGEPRSLEEKIEDVGLLHALNKASGAISLHIPWDIPTD
                     RNAIKALAAQHGLKFDAVNSNTFQNQADQEHSYKFGSLQNVNKAVRKQAIAHNIEVIQ
                     QGIELGSESLTVWLADGSCFPGQLNFRKAYQNTLESLEEIYAALPSNWKLFLEYKCAE
                     PNFYSTTVADWGQSYSYVKKLGDKAKTLVDLGHHLPNANIEQIVSLLLMEDKLGGFHF
                     NDSKYGDDDLTAGALKPYQLFLIFNELVEGMDARGMNHAKDLGWMIDASHNVKDPMED
                     LLQSVEAIMIAYAQALSVDRKALEIAQEENDVVKAQEILQNAFRTDVRALVAEARLRS
                     GAALNPVGLYRDLAVRNNLIGERGLKTVATGL"
     gene            complement(2177..>2631)
                     /locus_tag="PLQ70_11580"
     CDS             complement(2177..>2631)
                     /locus_tag="PLQ70_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE35301.1"
                     /translation="GQFIVSKAGIEVMRKQGFGGDIVNIVSKNAVVAGPNNPGYGSAK
                     AAQAHLTRLMAAELGADKIRVNTVNPDAVISDSNIWSGGWAEGRAKAYGITVAELPAY
                     YAKRTLLNEIILPDDIANACFAFVGGLLGKSTGNALNVDGGVSMGFYR"
BASE COUNT          828 a          526 c          416 g          861 t
ORIGIN      
        1 tttgaatggg tggcaatttg tctattattc cttcattggt aacccattta tgatatttca
       61 ttttcttgaa attccaaaga ccggtgtgac aaccaatact cgtaatatca gtaaaatagt
      121 gatgcgtcaa aacagaactt aaatattgtg gcaaatgcag gcaatatttt actttggcaa
      181 acaattcagg tttttcttct ttgagtctat aaatttgcat tccagaattc aaacttccta
      241 aaataggcga agcagtttta acggcaaact tcttttttcc tttgtattta ggataaaagt
      301 tttctaataa agtttctgga tacggtttca aataattgta gagcggtgtc aaactcttgc
      361 cttgctcatc tagataaaca aaactagcac cgtaggtact gaaattgata gctttcaaga
      421 catactcctt cagctctttt agttcagtca atcgatcggc tacccattgg ctaagttcta
      481 caatattttc gcagggaaaa ccatcttcat ctaccgtttc ggcaagaatt actgattttt
      541 cccaaactat ttgataattt tcgttgaaca aaaaaacctt tttgtttgtt ttcccaatat
      601 caaaaatcgc aactacattc attttttagt attttataaa ctgtccgaaa aacggatttt
      661 attcctatta ttattcaaga attgcacaaa ctcaacactt tttttcataa aaaaacgtgt
      721 tcaatttgtg caatttctat ttattcacaa agaacttaat tacaagccag tagctactgt
      781 ttttaatcct ctttcgccaa taagattatt tcttactgca agatcacggt acaaaccaac
      841 tggattcaat gctgctccag aacgcaaacg cgcttcggca actaaagctc taacatctgt
      901 acggaaagcg ttttgcaaaa tctcttgcgc ttttaccaca tcgttttctt cttgtgcaat
      961 ttccaatgct tttctgtcaa cagaaagtgc ttgagcataa gcaatcataa tagcttcaac
     1021 agattgtaat aaatcttcca tcggatcttt cacgttgtga gaagcatcga tcatccagcc
     1081 caaatctttg gcgtgattca ttcctctagc atccattcct tctaccaatt cgttgaaaat
     1141 caagaatagt tggtaaggct ttaaagcacc agctgttaaa tcgtcatcac catatttaga
     1201 atcattgaag tggaaacctc ctaatttgtc ttccattaat aacaatgaaa cgatttgttc
     1261 aatattagca tttggcaaat ggtgccccaa atcgactaat gtttttgctt tatcccctaa
     1321 tttttttaca taagaatacg attgtcccca atcggctact gttgttgagt agaaatttgg
     1381 ttcggcacat ttgtactcca aaaataattt ccaattagaa ggtaaagcag catatatttc
     1441 ttctaaactt tctaaagtgt tttggtaggc tttacggaaa ttcaattgac ctggaaaaca
     1501 agaaccatcg gcaagccaaa cagtcaatga ctccgaaccc aattcaatac cttgttgaat
     1561 tacttcgata ttgtgggcaa tggcttgttt gcgcaccgct ttgtttacgt tttgcaaaga
     1621 accaaattta taagaatgct cttgatcggc ttggttttga aacgtgtttg aatttactgc
     1681 atcaaatttc aatccgtgtt gtgctgccaa agctttaatg gcattacgat cggttggaat
     1741 atcccaagga atgtgcaatg agatagctcc agaagccttg ttcaaagcgt gaagcaaacc
     1801 tacatcttcg attttttctt ctaaagaacg aggttcacca ccacccggaa aacgtccaaa
     1861 acgagttcct cctgttccta aagcccaaga tggaatggcg atttgaaaat cgattaattt
     1921 ttgaataata ttttcagttt cagcaatttc agaagcggtg aaaaccaatt tattttgatg
     1981 agactttagt aaaccctcat tatgtgaagc tattttgttt gaatcaatta gcataatatt
     2041 ttttttaaga tagttaaata gaaaaggtat tgtaatgtgc agtaaaactt tagatcataa
     2101 ttatctgaaa aatataaatc taacgcccga agaactcgaa cgttagataa acaaattact
     2161 aatctaaaat ttaatattat ctataaaagc ccatagatac accaccgtca acattcaaag
     2221 cgtttcctgt tgatttaccc aacaagccac ctacgaatgc aaaacaagca ttggcaatat
     2281 catctggcaa tataatttca tttaataaag tacgttttgc gtagtatgca ggcaattcag
     2341 ccacagtaat accgtaagct ttagcacgtc cttcagccca tcctcctgac caaatgtttg
     2401 aatcagagat tacagcatca gggttaacag tgtttacacg aattttatca gctcctaatt
     2461 cagcagccat taaacgagtt aagtgagctt gagccgcttt ggccgaacca taaccaggat
     2521 tgtttggtcc agcaaccact gcatttttag atacaatgtt tacgatatcg ccaccaaaac
     2581 cttgtttgcg cattacttcg attccagctt tagaaacaat aaattgtcct t
//
LOCUS       DAOSNN010000299         2597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_39651_length_2597_cov_3.734461, whole genome shotgun sequence.
ACCESSION   DAOSNN010000299 DAOSNN010000000
VERSION     DAOSNN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39651_length_2597_cov_3.734461"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="PLQ70_11585"
     CDS             <1..1363
                     /locus_tag="PLQ70_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HQE35302.1"
                     /translation="PKDFVAQEEVSLSTTPSFINGTIEPRFAALRAFLIADETDYIVM
                     QGGLTRSSAVKGKFEISNQLGGISKDTWIISDSPTEYHEKSVERKNTNNQLNNSLTSR
                     NAENLFWLGRLCERTMALRSFLKIILNSLNENVTKSDNKQPDFLVVLLKSLTHLTQTY
                     PGFVGKEPKLGEVFDNEAIFENPIEELLLLINDSTKPGSVVYNLQSMLNTINQVSEKW
                     NHDTRRIINLVEDSLFALKNTNTNNITFVNHGLDKLHIRLFSFYGNIYETLPRDNGFY
                     LLETGKNVERILSLLSVFRSTLNFKKKEEEEALLMEAILENHHLLAKYRNIYKSHLSL
                     KAVVNMVFLEKNLPYTLSYLLDTLCYYLSKLPKANDPNRLSIAEKSALEACTIVKLID
                     ADDLIQVDEFTQFRPKLDKTISQVFDLICQVSTNLSSLYFNHSVMQHSALDTLENRDT
                     DEI"
     gene            1353..2219
                     /locus_tag="PLQ70_11590"
     CDS             1353..2219
                     /locus_tag="PLQ70_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQE35303.1"
                     /translation="MKYKLKHKTLYTYANAVHNYQSILCLQPRNSAKQICTNFQLEIE
                     PKPSKIYSRTDYFGNIQHYFSLHESHKSLKVTVSSEIEVLKNEVQPLSTITCEEARVK
                     FTTDRPLKIEVLQYQLPSQFISWDEEIVAFAETCLRPNVPLFEAVLDLIKKIYTEFQF
                     KSGATNINTPLKTVLKERKGVCQDFSHLAIASLRSVGIPAKYVSGYIETLPPKGKVKL
                     EGSDASHAWISVYIPEMGWCEFDPTNNMIPQQRHIVTAFGRDFADVSPLKGIIFSSGE
                     QKIKVEVDVIPL"
     gene            2333..>2597
                     /locus_tag="PLQ70_11595"
     CDS             2333..>2597
                     /locus_tag="PLQ70_11595"
                     /inference="COORDINATES: protein motif:HMM:NF036911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamine glutaminase family protein"
                     /protein_id="HQE35304.1"
                     /translation="MPYDYQQANCHNISHYISMLLESKGYQCAKIWAFAPAVYSTSNS
                     KLISFIDEKKISPNGTIDWRYHVAPVIEVQIGEKIRKMVIDPGL"
BASE COUNT          895 a          477 c          431 g          794 t
ORIGIN      
        1 cccaaaagat tttgttgccc aagaggaagt aagtttatca acaacgccat cctttatcaa
       61 cggaacaatc gaacctcgat ttgccgctct tcgtgcattt ttaattgccg atgaaacaga
      121 ttatatcgta atgcaaggtg gtttaacgag aagttcagcc gtaaaaggaa agtttgaaat
      181 ttcaaaccaa ttgggaggga tttcaaaaga tacttggatt atttcggatt cgccaacaga
      241 atatcacgaa aagtctgtag aacgaaaaaa caccaacaac caactcaaca actctttgac
      301 cagccgaaat gctgaaaatt tattttggtt aggacgtttg tgcgaaagaa caatggcatt
      361 gcgtagtttc ctgaaaatca tcttgaatag tctcaacgaa aatgtaacta aaagtgataa
      421 taaacaaccc gattttttag tcgttttact taaatctttg acccatctta cacaaactta
      481 tcctggtttt gtggggaaag agcctaaact aggagaagtc tttgacaacg aagccatttt
      541 tgaaaaccct atagaagaat tacttctttt gatcaatgat tcgaccaaac ccggttcggt
      601 agtttataat cttcagtcta tgctcaacac cattaatcaa gttagcgaaa aatggaatca
      661 cgacacaaga cgaattatca acttggtgga agacagtctt tttgccctaa aaaacaccaa
      721 cactaataac ataacatttg taaaccacgg tttagacaaa ttacatattc gcctgttttc
      781 attttatggc aatatctatg aaactttgcc tcgtgacaat ggtttttact tgttagaaac
      841 tggtaaaaat gtggaacgca ttttatcctt gctttcggtt tttcgatcta cattaaattt
      901 caagaaaaaa gaggaagaag aagccctttt gatggaagct attctagaaa atcaccattt
      961 attggccaaa tatcgaaata tttacaaatc gcatttgagt ttgaaagccg tagtcaatat
     1021 ggtgtttttg gaaaaaaact tgccttatac cctttcttat ttattggata ctctttgcta
     1081 ttatttgtca aaattgccca aagccaatga ccctaatcga ttgagtattg ccgaaaaatc
     1141 agctttagaa gcttgtacta tagtcaaact aattgatgca gatgacttaa tacaagtaga
     1201 tgaattcact caatttcgac cgaaattaga taaaacaata tcgcaagttt ttgatctgat
     1261 ttgtcaagtt tctaccaatt tatccagttt gtattttaat cattctgtga tgcaacattc
     1321 cgctttagac acccttgaaa atagagatac agatgaaata taaactaaaa cataagactc
     1381 tttatactta tgcaaacgca gttcacaatt atcaaagtat tttgtgtttg caacctcgta
     1441 attcagccaa acaaatttgc acaaatttcc aattagaaat tgaaccaaaa ccttctaaaa
     1501 tatattcacg aaccgattat tttggcaata ttcagcatta tttttcgctt cacgaatcgc
     1561 ataaatcctt aaaagttaca gtttctagcg aaattgaagt gttgaaaaat gaagttcaac
     1621 ccttgagcac catcacttgt gaagaagcta gggtgaaatt caccaccgat cgtccactga
     1681 aaattgaagt tttacaatat caattaccta gtcaattcat cagttgggac gaagaaattg
     1741 tagcttttgc agaaacttgt ttacgaccca atgttcccct ttttgaagca gttttagatt
     1801 taatcaaaaa aatatatacc gaattccaat tcaaatcggg tgcaacaaat atcaatacac
     1861 cactaaaaac agtacttaaa gaacgtaaag gtgtttgtca agatttctcc catttggcaa
     1921 ttgcgagttt gcgaagtgtt ggaattccag caaaatatgt aagcggttat attgaaaccc
     1981 tgccaccaaa aggaaaagta aaattagaag ggtctgatgc ttctcacgct tggatatctg
     2041 tttacattcc agaaatgggc tggtgcgaat ttgaccccac caacaacatg attccgcaac
     2101 agcgccatat tgtaacggca ttcggaagag attttgccga tgtttcgcct ctaaaaggaa
     2161 ttatttttag ttctggcgaa caaaaaataa aagttgaagt agatgttata cctttgtaat
     2221 aaatcaaaac aattgcatca tcaaaaagca tccactttta acgtcgcata aagcatcaat
     2281 actagatcta aatacagcaa aatctttatt tgctgaaatt acagaatctg gaattcctta
     2341 tgactatcaa caagctaatt gtcataatat ttcgcattat attagtatgc tattagaatc
     2401 aaaagggtat caatgtgcca aaatttgggc ttttgctcca gctgtttact ccacttccaa
     2461 tagtaaattg atttctttta tcgatgaaaa aaaaatatct ccaaacggaa ccattgattg
     2521 gaggtatcac gttgctcccg tgattgaagt ccaaattggg gagaaaatac gtaaaatggt
     2581 gattgaccca ggtttgt
//
LOCUS       DAOSNN010000300         2564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_40335_length_2564_cov_4.055401, whole genome shotgun sequence.
ACCESSION   DAOSNN010000300 DAOSNN010000000
VERSION     DAOSNN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40335_length_2564_cov_4.055401"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /locus_tag="PLQ70_11600"
     CDS             <1..1727
                     /locus_tag="PLQ70_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014789950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HQE35305.1"
                     /translation="QNRLEQHYKDMQDIEFTIEKGKLFMLQCRIGKRNGVAAVKIASD
                     MYNSKLIDAKTAVMRVAPNQLVELLLPMLNPDIQKITRPIAKGLPAGPGGAKGRVVFS
                     SNDAVEWASRGEKVILVREETSPEDVDGMHKSQAILTTKGGMTSHAALVARGWGKCCI
                     VGCNDIQINSETKSFKANDGTSIKEGDWLSLDGTKGLVYLGSMELVDVDLDKNQSYKN
                     LMKLVDKTKVLGVRTNAETPGDAEQGVKFGAEGIGLFRTEHMFYGEGSDQPLFLLRKM
                     IVSKTENERRDALNGLSVYVKKDIKKTLEVMSGHPVTIRLLDPPLHEFVPHDKQKLIQ
                     LSKELGISMSVLNRRVLALHENNPMLGHRGVRLGVSYPEITEMQIRAIFEATAELIQE
                     GKKALPEIMVPVTCSKNELKHQKEIVNEVYKETCEKMGIKKIPYLYGTMIEIPRAALK
                     ADVMAEEADFFSFGTNDLTQMSFGFSRDDIGGFLPKYLDLGILPSDPFQTIDQDGVGE
                     LIRLGVERGRKTKPNLKVGICGEHGGDPDSVVFCHRMGMNYVSCSPFRVPIARLAAAQ
                     AALEYPRE"
     gene            1956..>2564
                     /locus_tag="PLQ70_11605"
     CDS             1956..>2564
                     /locus_tag="PLQ70_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQE35306.1"
                     /translation="MKKILLPILALAVYSNGISQENKKPSSEKLLKELSEKGCKCVDS
                     ISTYNRPDNEIAMDIGECIDKQVGPYQLGSKLIGIDVSKEKAPVVDGKKQIDISFNTN
                     ENSEEYKKYYYEIERYMMANCSSVKEKMASNEKQSAKSFSKNKKAIEYYNKGLDEVEK
                     DNSQKAVEYFGKAVKEDPEFAFAWDNLGVNYRRLNNYDKAIEC"
BASE COUNT          918 a          383 c          531 g          732 t
ORIGIN      
        1 ttcaaaatag gttggaacaa cattataaag acatgcaaga tattgagttt acgatcgaaa
       61 aaggaaaact atttatgttg caatgccgaa tcggaaaacg caatggagtt gctgctgtaa
      121 aaattgctag cgatatgtat aattcaaaat taattgatgc caaaacggcg gtaatgcgtg
      181 tggcgccaaa tcaattagta gaattattac tgcctatgct aaatcctgat attcaaaaaa
      241 ttacacgacc tattgcaaaa ggattaccag cgggtccagg tggtgccaaa gggcgtgtgg
      301 ttttctcgtc taatgatgct gttgaatggg ctagtcgagg cgaaaaagta atcttagtca
      361 gagaagaaac ttctccagaa gatgtggatg gaatgcacaa atcacaagct attcttacta
      421 caaaaggcgg aatgacttct cacgctgcat tagtagcaag aggctggggg aaatgttgta
      481 ttgtgggttg taatgatatt caaattaaca gtgaaacaaa gtcgttcaaa gccaacgatg
      541 gtacaagtat caaagaaggc gattggttaa gtttagacgg tacaaaaggg cttgtttatc
      601 tcggaagtat ggaattggtt gatgtagatt tagacaaaaa tcaatcctat aaaaacttaa
      661 tgaaattggt agataaaacc aaagtattag gagttcgtac caatgccgaa acacctggcg
      721 atgccgaaca aggcgtgaaa tttggagccg aaggaattgg tttattccga accgaacaca
      781 tgttttatgg cgaaggaagc gaccaacctt tgtttttatt gcgcaaaatg attgtgagta
      841 aaaccgaaaa tgaaagaaga gatgctttga atggtttgtc tgtttatgtg aaaaaagata
      901 ttaaaaaaac attggaagta atgagtggtc atcccgtaac cattcgatta ttagatcctc
      961 ctttgcacga atttgtgcct cacgacaaac aaaaattaat tcagctgagc aaagaattag
     1021 gaatcagtat gagcgttttg aacagaagag ttttggcttt gcatgaaaat aatccaatgc
     1081 taggacatcg tggtgtacgt ttgggagtaa gttatcctga aattaccgaa atgcaaatcc
     1141 gagccatttt tgaagctact gccgaattga ttcaagaagg caaaaaagca ttgcctgaaa
     1201 ttatggtgcc ggtaacttgt tccaaaaatg aattaaaaca tcaaaaagag attgtaaacg
     1261 aagtgtataa agaaacttgc gaaaaaatgg gaatcaaaaa aatcccctat ttgtatggaa
     1321 caatgatcga aatacctcgc gctgctctca aagccgatgt gatggcagaa gaagccgatt
     1381 tctttagttt tggaaccaac gatttaactc aaatgagttt cggtttcagt cgggatgata
     1441 ttggaggatt ccttccaaaa taccttgatt taggaattct tccttccgat ccatttcaaa
     1501 ctatcgacca agacggtgtt ggcgagttaa ttcgacttgg agtagaaaga ggtcgtaaga
     1561 caaaaccaaa tctaaaagta ggtatttgtg gcgaacacgg tggcgatcct gatagtgtgg
     1621 ttttctgcca tcgaatgggg atgaattatg taagttgttc tccatttcgt gtgccaattg
     1681 caagattagc agcagctcag gcggctttag aatatccaag agaataattt agaatacgca
     1741 tttataatag tgggaaacgg catccaaaat taggttgccg ttttttctat tttcaagccc
     1801 aaaaaaaaag atttgtttat tgaaattttc aattttagta aaaattaatt ggttaaattt
     1861 gattttccga atgagtcaat ttctaattga tttttcgcta ttttttacca agataaaatg
     1921 aattgatgta taacttttta ataattaaaa tttaaatgaa aaaaatattg cttccaatac
     1981 ttgcacttgc agtttattca aatggaattt ctcaagaaaa taaaaaacca agttcagaaa
     2041 aacttttaaa agaactttcc gaaaagggtt gtaaatgtgt cgattctatt tctacttaca
     2101 atagaccgga taatgaaatt gcaatggata ttggcgaatg tattgacaaa caagttggtc
     2161 cctatcaatt gggttcaaag cttataggga ttgatgtttc gaaagaaaaa gctccagtag
     2221 ttgatggaaa aaagcaaatt gatatttcct ttaacactaa tgaaaattca gaagaataca
     2281 aaaaatatta ttacgaaatc gaacgctata tgatggcaaa ttgctcttct gtaaaagaga
     2341 aaatggcatc taacgaaaag caaagtgcta aatcattttc taagaataaa aaagccatag
     2401 aatactataa taaaggattg gatgaagtcg aaaaagataa ttcacaaaaa gcagttgaat
     2461 actttggaaa agcagtcaaa gaagaccccg aatttgcctt tgcttgggat aatttgggtg
     2521 tcaattatag aaggttaaac aattatgaca aagccatcga atgt
//
LOCUS       DAOSNN010000301         2562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_40370_length_2562_cov_3.632230, whole genome shotgun sequence.
ACCESSION   DAOSNN010000301 DAOSNN010000000
VERSION     DAOSNN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40370_length_2562_cov_3.632230"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLQ70_11610"
     CDS             <1..713
                     /locus_tag="PLQ70_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HQE35307.1"
                     /translation="TWPDGLTDIQHRMADIEHHMLKFLDNHDEQRLASPEFAGTPQKG
                     KPLMVVSATISSAPTMIYFGQEVGEAANENAGFGTHSRTSIFDYIGVPNHQRWMNGGK
                     FDGGQLSANEKELRDFYKRLLNFTINSSALMGNFQEIQTANRNITPGYDIAIYAYTRW
                     SDTQKLIVVTNFYSLASSNFELRIPTDIIQKWNLKDGTYTITDQLYHQKTTQLRVENG
                     EGKAQISIGPSESFIFQL"
     gene            complement(776..1546)
                     /locus_tag="PLQ70_11615"
     CDS             complement(776..1546)
                     /locus_tag="PLQ70_11615"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HQE35308.1"
                     /translation="MKKISLILTYLFLLTILISCDNKKYTEVVEVPLPSAEEKVIMGM
                     PDDVKAEEGSFQLEKLPYSYDALAPNISVFTMENHYSKHYLTYTNNLNKAIAGTPLEN
                     LTIEEVLAKLDPSIPEIKNNAGGYYNHSLFFKCMAPKAGGQPKDTLAGAITKKFGSFE
                     NFTTAFKDEAAKQFGSSWVWLIVDRSGTLQITSTQNQDNPLMRNATIPGKPILALDLW
                     EHAFYLDYQYKRKNYVDAFFNVINWKKVGENYEETLKK"
     gene            complement(1555..1824)
                     /locus_tag="PLQ70_11620"
     CDS             complement(1555..1824)
                     /locus_tag="PLQ70_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HQE35309.1"
                     /translation="MTNKDLDTRFQEAVEIASNMTQASLPQDVQLRLYAFYKQATFGS
                     LSFNHSESADLRNAFKTNAWIQISHLSIDEAKEKYIEIINSIIQK"
     gene            complement(1824..2477)
                     /locus_tag="PLQ70_11625"
     CDS             complement(1824..2477)
                     /locus_tag="PLQ70_11625"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HQE35310.1"
                     /translation="MFHKEGAQSILIGTVVTAIVLLLTDKFIDTNWIKMSIQIITFLL
                     LIIILQFFRNPKRNFILNENEIISPVDGKVVVIEEVYEGEYFKEKRIQVSIFMSPINV
                     HVTRYALSGLVKFSKYHPGKFLVAWHPKASEENERTTIVIENKTFGEVLYRQIAGALA
                     KRIVNYAQEGMQVIQGEDAGFIKFGSRVDLFLPLGTPINVVLNQKAIGGKTIIATKS"
     gene            complement(2467..>2562)
                     /locus_tag="PLQ70_11630"
     CDS             complement(2467..>2562)
                     /locus_tag="PLQ70_11630"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQE35311.1"
                     /translation="PGHGGILDRLDSVIFVAPIIFLFYQILNYVS"
BASE COUNT          807 a          419 c          445 g          891 t
ORIGIN      
        1 aaacttggcc tgatggatta acagatattc agcatagaat ggctgatatt gaacatcata
       61 tgttaaagtt tttggacaat cacgacgaac aacgtttggc aagcccagaa tttgcaggta
      121 caccacaaaa agggaaacca ttgatggtcg tttcagccac cataagttct gcaccaacga
      181 tgatttattt tggtcaagaa gtaggggaag cagcaaatga aaatgctggt tttggaactc
      241 attccagaac atctattttt gattatatag gagttcccaa tcatcaacga tggatgaacg
      301 gagggaaatt tgatggagga caactttcgg caaacgaaaa agagttgcgg gatttttata
      361 aaagattatt gaatttcacc atcaatagtt cagccttaat ggggaatttt caagaaattc
      421 aaactgccaa tcgaaacata actccaggtt atgatatagc gatttacgct tatactcgtt
      481 ggtctgacac gcaaaaattg attgttgtaa ctaattttta ttcactagca tctagtaatt
      541 tcgaacttcg aattccaacc gatattattc aaaaatggaa tttgaaagat ggaacttata
      601 ctatcacgga tcaattgtat catcaaaaaa ctacgcaatt aagagtagaa aatggtgaag
      661 gaaaggctca aattagtatt ggtccgtcgg aatcgtttat ttttcagttg taaaaagata
      721 aaaaaaacgg gttaaatgta tcaaacattc aacccgtttt ttttaattgc tattgctact
      781 tttttaaggt ttcttcataa ttttcgccca cttttttcca attgattaca ttaaaaaaag
      841 catcgacata attttttctt ttgtattggt aatccaagta gaaagcgtgt tcccaaaggt
      901 ctaaagccag aattggtttt ccaggaattg ttgcatttcg catcaaagga ttgtcttgat
      961 tttgggtgct tgtaatttga agtgttcccg atctatctac aatgagccaa acccaactag
     1021 agccaaattg ttttgcagct tcatctttga atgctgtggt aaagttttca aaagagccga
     1081 atttttttgt aatagctcct gccaaagtgt ctttgggttg gccaccagct tttggtgcca
     1141 tacatttaaa aaaaagcgaa tggttgtaat aaccgccagc attatttttt atttcaggaa
     1201 tactcgggtc taattttgcc aacacttctt cgatagtaag attttctagt ggagttccag
     1261 ctatggcttt attcaaatta ttggtgtaag tcaaataatg ttttgaataa tggttttcca
     1321 tagtaaaaac tgagatattt ggagccaaag catcatacga ataaggtagt ttttctaatt
     1381 gaaatgaacc ttcttcggcc ttgacatcat caggcattcc cataattacc ttttcttctg
     1441 ctgagggtaa aggcacttct acaacttcgg tatatttttt attatcgcat gaaattaaaa
     1501 ttgtgagtag aaataaatag gttagaatta aactgatttt tttcatagtt atcactattt
     1561 ttgaataatg gaattgatta tttcgatgta tttttcttta gcttcatcaa tagataaatg
     1621 acttatttgt atccacgcat ttgttttgaa agcatttctt aaatcagcac tttcagaatg
     1681 attgaaactt aaagaaccaa aagtggcttg tttataaaag gcataaagtc ttaattggac
     1741 atcttgcggg agtgatgcct gagtcatatt tgaagcaatt tcaaccgctt cttgaaatcg
     1801 tgtatctaag tctttatttg tcattaagat tttgttgcaa taattgtctt tcctccaatt
     1861 gctttttggt ttagaactac attaattggt gttcccaaag gtaaaaaaag atctactcta
     1921 gaaccaaatt ttataaaacc tgcatcttca ccttgaatga cttgcattcc ttcttgggca
     1981 taattgacga ttctttttgc tagtgctcct gctatttggc gataaagaac ttctccaaag
     2041 gttttatttt cgattacaat ggttgttctt tcgttttctt cgctcgcttt tggatgccaa
     2101 gctaccaaga attttccagg atggtatttg ctgaatttca ccaaaccact aagagcataa
     2161 cgggttacat gtacatttat tggtgacata aatatagaaa cctgaattcg tttttctttg
     2221 aaatattcgc cttcatacac ttcttcaatt acaactactt ttccatctac tggagaaata
     2281 atttcatttt cattgagtat aaagttgcgt tttgggtttc tgaaaaattg caaaatgata
     2341 atcaataaca aaaaagttat gatttgaatg gacattttaa tccaattggt gtcaataaat
     2401 ttatctgtta ataaaagtac aattgctgta acaacagttc ctattagtat tgattgagcg
     2461 ccttctttat gaaacatagt ttagaatttg gtaaaataaa aatattattg gtgctacaaa
     2521 tataacacta tctagtcgat ctagaatgcc tccgtgtccc gg
//
LOCUS       DAOSNN010000302         2554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_40546_length_2554_cov_5.253301, whole genome shotgun sequence.
ACCESSION   DAOSNN010000302 DAOSNN010000000
VERSION     DAOSNN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40546_length_2554_cov_5.253301"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1273)
                     /locus_tag="PLQ70_11635"
     CDS             complement(206..1273)
                     /locus_tag="PLQ70_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HQE35312.1"
                     /translation="MTSKLFSPVQIKSLTFKNRITISPMCQYSSTDGFATNWHLVHLG
                     SRASGGAALIIQEATAVCPEGRISPADLGIWKDEQIEKMKTINQFIISQNSIPGIQLA
                     HAGRKASVSEPWNGNKKLDFAQGGWQTVSSSALPYHENEPFLPVALDKIGIQKVISDF
                     KSATKRAVEAGFQVLEIHGAHGYLLHQFLSPLTNNRTDEYGGSFENRIRFTLEILEAV
                     QSEWPENLPLFVRISATDWAEGGWDIEESVQLSKILKEKGVDLIDVSSGGLVSHQQIA
                     IGPGYQVSFAEKIKKETEILTSAVGLITTSSQAEAIVSDGKADLVMFARESLRNPNLA
                     LDFAKELGVDVAWPKQYERAK"
     gene            1475..>2554
                     /locus_tag="PLQ70_11640"
     CDS             1475..>2554
                     /locus_tag="PLQ70_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQE35313.1"
                     /translation="MEIVIKLSQFLLSLSLLIILHELGHFIPSKLFKTRVEKFYLFFD
                     VKYSLFKKKIGETEYGIGWLPLGGYVKISGMIDESMDKEQMSLPPQPWEFRSKPAWQR
                     LIIMLGGVTVNFILAFIIYIGMTFFYGEQYIANSEAKNGIWITNPVIEKIGLKTGDKI
                     IAVDGKPVERFDDINGEILLGKEVTLERAGKVQTIKLPVDFIAKLMDGRKIGIASLRL
                     PFIVSEVPSDSPNKNNLKEKDILISLNKKPIAFADEAIAALDGLKGKTVPAIVKRDNI
                     EVPVNIIINDSAKLGISYAAKLPYSNIEKLGLYKVSKETYSFVESIPVGIEQGKEQLV
                     NYGKQLKMIFNPSTGAYKGVGGFAAI"
BASE COUNT          823 a          431 c          467 g          833 t
ORIGIN      
        1 aaacttttcc cgacattccg taagataaga gtgctgtttt tattttttgc atttttttgt
       61 ttttaaattc tatattgatt gcagattttt gattaacgat ttttgactgc agaagtttaa
      121 ctcctcgtaa aatcaataat ctcctattta tcaacgccaa atctcaaatc tgaaatcgtt
      181 aatcatcatt caaaaatcat ttaatttatt tagctctttc gtattgtttt ggccaagcca
      241 cgtcaacacc gagttctttg gcaaaatcca aagccaaatt agggtttctc aaagattctc
      301 tagcaaacat aaccaaatcg gcttttccat cagaaacaat tgcttctgct tgagaagagg
      361 tagtgattaa acctactgca cttgtcaaaa tttctgtttc tttcttgatt ttttctgcaa
      421 atgaaacttg ataaccgggt ccaattgcaa tttgttgatg agataccaat ccgcccgaag
      481 aaacgtctat taaatctacg cctttctctt ttaatatttt cgaaagttgt acggattcct
      541 caatatccca acctccttca gcccaatctg tagctgatat tctaacaaac aaaggcaaat
      601 tttctggcca ttctgattgt accgcttcga gaatttcaag tgtaaatcga atcctgtttt
      661 cgaaacttcc gccgtattcg tccgttctat tattcgttaa aggcgacaaa aattgatgta
      721 gtaagtaacc gtgtgctcca tgaatttcaa gaacttgaaa acccgcttca acggctcttt
      781 tggttgccga tttgaaatcg gaaatcactt tttgaattcc aattttgtct aaagcaactg
      841 gcagaaaggg ttcgttttcg tgatagggta gggcgcttga gctaacggtt tgccatccgc
      901 cttgagcgaa atctaatttt ttgtttccat tccaaggttc tgaaacgctt gcttttcttc
      961 cagcgtgtgc caattgtatt cctggaattg aattttgtga aatgatgaac tggttgatgg
     1021 ttttcatttt ttcgatttgt tcgtctttcc agattcccaa atcagcagga gaaattcgtc
     1081 cttcgggaca aactgcggtc gcttcctgaa tgattaaggc ggcaccacca ctggctcgac
     1141 ttcctaaatg aacaagatgc caattggttg caaaaccatc tgtggatgaa tattgacaca
     1201 ttggtgaaat cgtgattcta tttttgaaag tgaggctttt tatttgaacg ggagaaaata
     1261 atttggatgt cataaaacga gttttaatta gaacaagcga aacatcaact caataatcga
     1321 gtttcaaaaa gtaaagttaa gggagaagca gcgaagatta aaagggaata gcgcatttat
     1381 taacaaataa ttatcgtctt gcctaaaagg aaatctttgg aactataata gaaaacctta
     1441 cttttgtgcg caatacagaa tttaaaaaca agaaatggaa atagttatca agctttctca
     1501 atttttattg agcttatcat tactgattat acttcacgag ttaggacatt ttattccttc
     1561 taaactattt aaaacccgag tagaaaaatt ctatttattt tttgacgtaa aatattcttt
     1621 gtttaaaaag aaaattggcg aaaccgaata cggaatcggt tggttgcctc ttggtggtta
     1681 tgtgaaaatt tcgggaatga ttgacgaaag tatggacaag gaacaaatgt ctttgccgcc
     1741 tcaaccttgg gaatttcgtt caaaaccagc ttggcaacga ttgattatta tgttgggcgg
     1801 agtaactgtt aatttcattt tggctttcat catttatatt ggaatgacat ttttttatgg
     1861 tgaacaatat attgccaact ctgaggcgaa aaatggaatt tggattacca atccagttat
     1921 tgaaaaaatt ggtttaaaaa caggtgataa aatcatcgct gttgacggaa aacctgttga
     1981 aagatttgat gatatcaacg gagaaattct tttaggtaaa gaagttactc ttgaaagagc
     2041 tggaaaagta cagaccataa agttacctgt tgattttata gctaaattga tggatggcag
     2101 aaaaataggt attgcatcct taagattgcc ttttattgtt tctgaagttc caagtgattc
     2161 tccaaataaa aacaacttaa aagaaaaaga cattttaatc agtttaaaca aaaaacccat
     2221 agcgtttgct gacgaagcta ttgctgcatt agatggatta aaaggaaaaa cggtacctgc
     2281 gattgtaaaa agagataata tcgaagttcc cgtaaacatt atcataaacg atagtgctaa
     2341 acttggaatt tcttatgctg caaaattacc atacagcaat atcgaaaaat taggtttata
     2401 caaagtgagc aaagaaacgt acagttttgt agaatctatt ccggttggaa ttgaacaagg
     2461 aaaagaacaa ttggtaaatt acggaaaaca actaaaaatg attttcaatc caagcactgg
     2521 tgcttataaa ggggttggtg gatttgctgc tatt
//
LOCUS       DAOSNN010000303         2525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_41164_length_2525_cov_7.501619, whole genome shotgun sequence.
ACCESSION   DAOSNN010000303 DAOSNN010000000
VERSION     DAOSNN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41164_length_2525_cov_7.501619"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2098
                     /locus_tag="PLQ70_11645"
     CDS             <1..2098
                     /locus_tag="PLQ70_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQE35314.1"
                     /translation="SEFNAIIHREIQNQPAPFIYERLGERYRHFFIDEFQDTSEMQWQ
                     NLIPLIDNATSSEIDGEKGTLMIVGDPKQSIYRWRGGKAEQFIELSKDHNPFNNPEKV
                     LEHLDKNYRSYSQIIEFNNEFFQLLSNEFEQEDYKDLYQNHSHQKSNDKKGGYVNISF
                     ISKIEENEDDEEVLDKTDLYLLATLNTIQKVQKQGFELKDIVILTRKRSHGIAVANYL
                     TEQGIPLLSSETLMIQNATEVRFIIYLLKYLKNNSDLEAKSRFLHYIAEKVQDQLEVH
                     DFIAQGMEHKKESEFEIWLTDFDISLSFQDIRKKSLYEAVELIVAKFLRSQNGNAYVQ
                     YFLDIVLDRDVRNQAGISDFLNYWDKNAEKFSIPSPEGKNAVRIMTIHKSKGLEFPVV
                     IMPFADEDYAKSPKSKLWIDAEKDEFGLPKVLVDNSKAVEGFGEAAKSVYVQKSQEEL
                     LDNINVLYVALTRAEEQLFVISELVNPKKDGEYPNNMASFFIKYLIHKREFEEGKLEY
                     EFGSAVKLSSESDKTEEVQLIQSVNEVLNPKNIKIAQRESFMWGTHQQEAIEYGNVVH
                     EILSFVKTKSDVDLAIIKAIESGLITFNQKDAVFKTIQEIVNHSELSICFEEGNQVLN
                     EQTIIRKEGKTIKPDRMVFTKNKEVYLLDYKTGTHNPKYQLQLENYQNAIEKMGYKVV
                     KKSLVYIGDQINVVNL"
     gene            2138..>2525
                     /locus_tag="PLQ70_11650"
     CDS             2138..>2525
                     /locus_tag="PLQ70_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQE35315.1"
                     /translation="MYGKIKDFLQSELQATEDNGIFKRERIITSPQGAEITVNGETVL
                     NFCANNYLGLSSHPEVIQAAKDTMDTHGFGMSSVRFICGTQDIHKTLEKKISDFYGTE
                     DTILYAAAFDANGGVFEPLLNENDAII"
BASE COUNT          888 a          406 c          499 g          732 t
ORIGIN      
        1 ttcggaattc aatgcgatta ttcatcgtga aattcaaaac cagccagcgc cttttattta
       61 tgaaagactg ggcgaacgct accgacattt tttcatcgac gaattccagg atacgtccga
      121 aatgcaatgg caaaacctga ttccgttgat tgacaacgcc acctccagtg aaattgacgg
      181 agaaaaagga actctgatga ttgtcggtga cccaaaacaa tccatttacc gttggcgtgg
      241 cggaaaagcc gaacaattta ttgagttgag caaagaccac aatccattca ataatcctga
      301 aaaagtttta gaacatttag acaaaaatta ccgttcgtat tcgcaaatca ttgaatttaa
      361 caacgaattt tttcaattgc tgtccaatga attcgagcag gaagattaca aagatttgta
      421 ccaaaatcat agccatcaaa aaagtaacga caaaaaagga ggttatgtga atatttcgtt
      481 catctccaaa atagaagaaa acgaagatga tgaagaagta ttggataaaa cggatttgta
      541 tttattggca acattaaaca cgattcaaaa agtgcaaaaa cagggttttg aactcaaaga
      601 cattgtgatt ttgactcgaa aacgcagtca cggaattgcg gttgccaatt atttgaccga
      661 gcagggaatt ccgcttctgt catcggaaac cttgatgatt caaaacgcca ccgaagttcg
      721 attcatcatt tatttgttga aatatttgaa gaacaattcc gatttggagg ccaaatctcg
      781 tttcctacat tatatcgccg aaaaagttca ggatcaatta gaagttcacg atttcattgc
      841 tcaaggaatg gaacataaaa aggaatctga atttgaaatt tggttgaccg attttgatat
      901 ttcactttcg tttcaagaca ttcgaaaaaa atcactttat gaagcagtgg agcttattgt
      961 tgcaaaattt cttcgttcgc aaaacggtaa cgcttatgtt caatattttt tggatattgt
     1021 tttagatcga gatgttcgaa atcaggcagg aatttcggac ttcttgaatt actgggacaa
     1081 aaatgccgaa aaattcagta ttccatcgcc cgaagggaaa aatgccgttc gaattatgac
     1141 cattcacaaa tcgaaaggtt tggaatttcc agtcgtgatt atgccttttg ccgacgaaga
     1201 ttatgctaaa agccctaaaa gtaaattatg gattgatgct gaaaaagatg aatttggttt
     1261 gcctaaagtt ttggtggaca acagtaaagc cgtggaaggt tttggagaag cggccaaatc
     1321 ggtttatgtc cagaaatcac aggaagaatt actggacaac atcaatgtct tgtacgttgc
     1381 tttaacccgt gccgaagaac aattgtttgt aatttcagaa ttagtaaatc ctaaaaaaga
     1441 tggagagtat ccaaataata tggcttcctt ctttattaaa tatttaatcc ataaaagaga
     1501 atttgaagag ggtaaattgg aatacgaatt tggtagtgcg gttaaattat cttctgaaag
     1561 tgataaaaca gaagaggtgc aattgattca atctgttaat gaagttttga atccgaaaaa
     1621 cattaaaatt gcccaacgag aatcgtttat gtggggaacg catcagcagg aagccattga
     1681 atatggaaac gtggtacacg aaattttgtc ttttgtaaaa acaaaatccg atgtcgattt
     1741 ggctataatc aaagccatcg aaagtgggtt aattactttt aatcaaaagg atgcagtttt
     1801 caagaccatt caagaaattg taaatcattc cgaactttca atttgttttg aagaaggtaa
     1861 ccaggttttg aacgagcaaa ccattattcg gaaagaggga aaaaccatca aacccgatag
     1921 aatggttttt actaaaaaca aagaagttta tttgttggat tataaaacgg ggacacataa
     1981 tccgaaatat caattgcaat tagaaaatta ccaaaatgcc attgagaaaa tgggttataa
     2041 agtggtgaaa aaatcactag tatatatagg agaccaaatc aatgtggtaa atttgtgaaa
     2101 gactaattct aaaacataaa tttctaaact ttttaatatg tatggaaaaa taaaagattt
     2161 tttgcaatct gaacttcaag ctacagaaga taacggtatt ttcaaaaggg aaagaattat
     2221 cacttcgccg caaggagctg aaattaccgt gaacggagaa acggttttga atttttgtgc
     2281 caataattat ttgggacttt cctcgcatcc tgaagttatt caggcggcga aagatacaat
     2341 ggacacgcac ggttttggaa tgtcgtccgt tcgatttatt tgtggcactc aagatattca
     2401 caaaaccttg gaaaaaaaga tttcggattt ctacggaacc gaagatacaa ttctatatgc
     2461 agctgcattt gatgccaatg gaggtgtttt cgagccttta ttgaatgaaa atgatgcgat
     2521 cattt
//
LOCUS       DAOSNN010000304         2489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_41986_length_2489_cov_3.589154, whole genome shotgun sequence.
ACCESSION   DAOSNN010000304 DAOSNN010000000
VERSION     DAOSNN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41986_length_2489_cov_3.589154"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLQ70_11655"
     CDS             <1..272
                     /locus_tag="PLQ70_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HQE35316.1"
                     /translation="LEIRVYTGKDGTFEFYEDENDNYNYEKGASSLIKFKWDETTKKL
                     TIDERKGTFDGMLKSRNFKIVFISSEKKSVVKTISYNGKAVKLGL"
     gene            326..>2489
                     /locus_tag="PLQ70_11660"
     CDS             326..>2489
                     /locus_tag="PLQ70_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HQE35317.1"
                     /translation="MKFKIIIILICVQSTFSQMQSFKLQDVKLTDGMFKNAQDVDLKY
                     ILELNPDKLLAPYWIDAGLPLKANRYGNWESSGLDGHIAGHYLSALAMMYASTGNVEL
                     KKRLDYMVNELALCQAKNGNGYVGGIPDGKVFWERIHKGDIDGSTFGLNNTWVPIYNI
                     HKLFAGLRDAYEITDNQQAKKVLIGLGDWFIELIKPLSTEQIQHVLDTEHGGINETFA
                     DLYIITKDKKYLDAAERLSHLKILNPLLEKQDKLTGLHANTQIPKVIGFEKIATLTHN
                     NDWEHAAEYFWQNVTQTRSVAFGGNSVREHFNPTNNFKEMLESNQGPETCNSYNMLRL
                     SKALFLDNNDVSYLDYYERTLYNHILSSQHPEKGGFVYFTPIRPNHYRVYSQPETSMW
                     CCVGSGIENHTKYGELIYSHSENDVFVNLFIPSELNWKEKGLQLIQKNNFPNENATEL
                     ILKLTKSQTFSLNIRYPKWAENIKILVNGKSETVTANPSQYINLNRKWKNGDKIAVQF
                     SMKTKLEYLPDGSNWAAFVRGPIVLAAKTSTTDLKGLFADDSRMGHETRGKLYPINEA
                     YALVDESKNYMSKLQSIDNMNFKMGSLTLQPFYQIHDARYQMYFQTFTQKEYETQKEI
                     QKQQEIALLALEAITVDKINCGEQQPEVDHQYKGEQSNSGYDDEKFWRSTRSYISYQL
                     QNKNKAGKYIEISTLNDIKLENLEITINGIEAKISSIEN"
BASE COUNT          906 a          402 c          458 g          723 t
ORIGIN      
        1 atttagaaat ccgagtatat actggaaaag acggaacttt tgagttctat gaggatgaga
       61 atgataatta taattatgaa aaaggagctt cttctttaat caaattcaaa tgggatgaga
      121 ctactaaaaa gctaacgata gacgaaagaa aaggaacatt tgacggaatg ttaaaaagcc
      181 gaaacttcaa aattgttttt attagttctg aaaaaaaatc agtagttaaa acaataagtt
      241 acaatggtaa agccgtgaaa ttgggattat gaaaaaggta aaaacagttt ctactgatta
      301 agaactaacc aaactaacta taaaaatgaa atttaaaatt ataataatcc ttatttgtgt
      361 tcaatctact ttttctcaaa tgcaatcatt caaattgcaa gatgtaaagc taacagatgg
      421 tatgtttaaa aatgcccaag atgttgacct aaaatacatt ttggaactca atcctgacaa
      481 actgctcgct ccctattgga ttgatgctgg cttgccattg aaagccaatc gatatggcaa
      541 ctgggaaagt tcaggtctag acggacatat tgctgggcat tatttgtcag ctttagcaat
      601 gatgtatgcc agtactggaa atgtcgaact taaaaaacgt ttggattata tggtcaatga
      661 actggctctt tgtcaagcaa aaaacggtaa tggttatgtt ggtggtattc ccgacggaaa
      721 agtgttttgg gagcgcattc acaaaggcga tattgatgga agcactttcg ggttgaataa
      781 tacttgggta cctatttaca atattcataa actttttgcg ggtttgcgtg atgcttatga
      841 aatcacagat aatcaacaag ccaaaaaagt tttgataggt ttaggcgatt ggtttattga
      901 actcataaaa ccactttcta cggagcaaat tcagcatgta ttggatacgg aacatggtgg
      961 catcaacgaa acttttgccg atttatacat cattactaaa gacaaaaaat accttgatgc
     1021 tgctgaaagg ctttcacatc ttaaaatttt aaatcctctt ctggaaaagc aagataaact
     1081 tacaggtctt catgccaata cacaaattcc aaaagtaatt ggttttgaaa aaatcgccac
     1141 cttaacacat aataacgact gggaacatgc agcagaatat ttttggcaaa acgtaactca
     1201 aactcgtagt gtggcatttg gaggaaacag cgttcgggaa cattttaatc cgaccaataa
     1261 cttcaaagaa atgctggaat ccaatcaagg tccagagact tgtaatagct ataatatgct
     1321 tcgtttgagt aaagctttgt ttttggacaa caatgatgtg agttaccttg attattacga
     1381 acgcacactt tacaaccata ttttatcgag ccaacatccc gaaaaaggag gttttgtata
     1441 tttcacaccc attcgtccaa atcattatag agtgtattcg caacccgaaa ccagtatgtg
     1501 gtgttgtgta ggttcgggta tagaaaacca tactaaatat ggagaattga tttatagcca
     1561 ttctgaaaat gatgtttttg taaatttatt tatcccttcg gaacttaatt ggaaagaaaa
     1621 aggattgcaa ttgatacaga aaaataattt tccaaacgag aatgccacag aactgatatt
     1681 gaaattgact aaaagccaga ctttttcttt aaatattcgc tatccaaaat gggcagaaaa
     1741 catcaaaatt ttggttaatg gaaaatcgga aactgttaca gcgaatccat cacaatacat
     1801 taatttgaat agaaagtgga aaaatggaga taaaattgct gtccaatttt ctatgaagac
     1861 caaattagaa tatcttccgg atggctccaa ttgggcagct tttgtacgtg gaccaatcgt
     1921 tttggctgcc aagacttcta ctacggattt aaaaggtctt tttgctgatg atagtcgaat
     1981 gggacacgaa accagaggta aattgtatcc gattaatgaa gcctatgctt tggtagatga
     2041 atcaaaaaat tatatgtcaa aattgcaatc tatcgataat atgaatttca aaatgggttc
     2101 attgacgctt caaccgttct atcagattca cgatgcacgt tatcaaatgt attttcaaac
     2161 ttttactcaa aaggaatacg aaacccaaaa agaaattcaa aaacaacaag aaatagcact
     2221 tttagctctt gaagccataa ctgtagataa aattaactgt ggcgaacaac aacccgaggt
     2281 cgatcatcaa tacaaaggcg agcaaagcaa ttcgggttat gatgatgaga aattttggcg
     2341 tagcacacgt tcttatattt cctatcagtt gcaaaacaaa aataaagctg gaaaatatat
     2401 cgaaatcagt actttgaacg atataaaact agaaaatttg gagattacca tcaatggaat
     2461 tgaagctaaa ataagctcca tcgaaaaca
//
LOCUS       DAOSNN010000305         2458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_42706_length_2458_cov_4.218061, whole genome shotgun sequence.
ACCESSION   DAOSNN010000305 DAOSNN010000000
VERSION     DAOSNN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42706_length_2458_cov_4.218061"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..>2458)
                     /locus_tag="PLQ70_11665"
     CDS             complement(47..>2458)
                     /locus_tag="PLQ70_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HQE35318.1"
                     /translation="NKITIDAPLTNALDEELSKSTVVSYIWSGRINNVGVENLSLKSD
                     YDSTNPKDEQHRWYGISITNAEDSWVRQVNFEQFAGGAVSILKTAKRITVEDCLALNP
                     ISEIAAFRRNTFYTEGTQTLFQRCNSELGYNDFVVGGYATAGPNVFLQCESHQPFSFS
                     GSVGSWATGILFDVSLIDGNAISFKNKEQDGRGLGWNVANSVIWETSASKIENYSPPT
                     ANNWAFGVWAQWAGNGHWKDVNNHINPRSLFYALLEQRLGKLPMKPQIMDLGNEPSSS
                     PTIEQAKVLTAAAYTLNETLKEYITKAATRNPIAIDFAKAKRIDGINTEVVINAKPVE
                     IKITNGFLTSSKGVLTGEIIDVPWWRGSLRESDISKSRPHITRFVPGHYGVGYTDNLD
                     ETLNFLVENNKGAIDHNYGLWYEQRMADHERIRRIDADVWAPFYEQPFDRSGQGIAWD
                     HLSKYDLTRYNAWYWNRLKTFAELAAAKNKILMNENYFQHNILEAGAHWASSAWRPEN
                     NINTTGLPEPPPYAGDKRIFLAEQFYDVKNTNIRKLHTAFIEKNLENFKDNANVLQMT
                     SAEYTGPLSFMQFWIDVVANYEKSHPNESKIALSATKDVQDAILNDEIRAKTVDVIDI
                     RYWYYKEDGTLYAPLGGVNLAPRQHARQLKVGKETDDQVYRAVREYREKYPNKAVLYS
                     TMGAPRFGWAALMGGASLTAIPKIELPAFYSEVGEMKLVSGNTFSDNLWILENKGKAY
                     LFYAKKAQDISIDLTNSKGNFEVYEINAEKGSVTKVASIGGGKKVTIASSDVKEKVLF
                     VVKKN"
BASE COUNT          677 a          541 c          399 g          841 t
ORIGIN      
        1 ttattgtttt ttacgttgat tcatttatta cccacggtta taatgactaa tttttcttca
       61 ctacaaaaag tactttttcc ttaacatcag aagaagcaat tgtaactttt tttcctcctc
      121 caatgctagc tactttggtt acacttcctt tttcagcatt tatctcatag acttcgaaat
      181 tccctttaga attagtcaag tctatcgaaa tatcttgtgc tttttttgca taaaaaagat
      241 aggctttccc tttattttct aatatccaaa gattgtctga aaaagtattt ccactaacca
      301 atttcatttc gccaacttct gaataaaatg cagggagttc aatttttgga attgcagtta
      361 aggaagctcc tcccattaat gctgcccaac caaatcgtgg tgctcccatc gtagaataca
      421 acaccgcttt attggggtat ttttcacggt attcacgaac agccctatag acttggtcat
      481 cggtttcttt acccactttc aactgacgag cgtgctggcg tggtgccaaa tttacgcctc
      541 ctaatggagc ataaagcgtt ccatcttctt tataatacca atatcgaatg tcaatcacat
      601 ctactgtttt tgctcttatt tcgtcattca aaatagcatc ctgaacatct tttgtagcac
      661 tcaaagctat tttggattca tttggatgcg acttttcgta gttggccacc acatcaatcc
      721 agaattgcat aaaagataaa ggtcctgtat attcggcact tgtcatttgc aatacgttgg
      781 cgttatcttt gaaattctct aaatttttct cgataaatgc ggtatgcaat ttacggatgt
      841 ttgtattttt tacatcataa aattgttcag cgagaaaaat ccttttatca ccagcataag
      901 gcggtggttc aggcaaaccg gtagtattga tattattttc tggacgccaa gcagaacttg
      961 cccaatgcgc tccagcttct aaaatattat gctgaaaata gttttcgttc attaaaattt
     1021 tattctttgc cgccgctaat tcagcaaagg ttttcaatcg attccaatac caagcattgt
     1081 atctcgttaa atcatattta ctcaaatgat cccaagcaat tccctggcca cttctgtcaa
     1141 atggctgttc atagaacggt gcccaaacat cggcatcgat tcttcggatg cgttcgtgat
     1201 ccgccattct ttgttcatac caaagtccgt aattatgatc tattgcccct ttattgtttt
     1261 caactaaaaa gtttagcgtt tcgtccaaat tatcggtgta acccacacca taatgacctg
     1321 gaacaaatcg agtaatatga ggtcttgatt ttgaaatatc agactcacgc aaacttcctc
     1381 tccaccaagg cacatctata atttctccgg taaggactcc ttttgatgat gttaaaaatc
     1441 cattcgtaat tttaatttca acaggttttg cgttaataac tacttcagta tttattccgt
     1501 caattcgttt tgctttggca aaatcaattg caattggatt tcttgttgcc gctttggtga
     1561 tgtactcttt taaagtttca tttaaagtat aggctgctgc ggtcaaaact ttagcttgtt
     1621 ctatcgtggg actcgaagaa ggctcattgc ccaagtccat aatttgcggt ttcatcggta
     1681 atttacccaa acgttgttcg agcaaagcat aaaataaact tcttggattg atgtgattgt
     1741 tcacgtcttt ccaatgtccg tttcctgccc attgcgccca aactccaaac gcccagttat
     1801 tggccgttgg cggactgtaa ttttctattt tggatgccga ggtttcccaa atgacgctat
     1861 tggccacatt ccagcccaaa cctctaccat cttgttcttt gtttttgaaa ctgattgcgt
     1921 ttccgtcaat tagcgacaca tcgaaaagaa ttccggttgc ccaacttcca acagatccgc
     1981 taaaactaaa cggctgatgc gattcgcatt gcaagaaaac atttggtccc gcagttgcat
     2041 atcctccaac cacaaaatca ttataaccca attctgaatt acaacgttga aacaaagttt
     2101 gtgttccttc tgtataaaag gtatttcttc taaaagctgc gatttccgaa ataggattca
     2161 atgccaaaca atcttcgaca gtaatccttt tggccgtttt taaaatagaa actgctccgc
     2221 cggcaaactg ttcaaaattg acttgtcgca cccaactatc ttccgcattt gtgatagaaa
     2281 ttccatacca acggtgttgt tcgtcttttg gatttgtcga atcataatcc gatttcaaac
     2341 ttagattttc gaccccaaca ttattgattc tgcctgacca gatataactt accacagttg
     2401 attttgacaa ttcttcatcc aaagcattag tcaaaggtgc gtcgattgta attttatt
//
LOCUS       DAOSNN010000306         2412 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_43774_length_2412_cov_3.689011, whole genome shotgun sequence.
ACCESSION   DAOSNN010000306 DAOSNN010000000
VERSION     DAOSNN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43774_length_2412_cov_3.689011"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PLQ70_11670"
     CDS             <1..913
                     /locus_tag="PLQ70_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HQE35319.1"
                     /translation="ITGEGELKAKMEVWKGWFKRPKGHMESLKRLYYLASYNKLMSER
                     LMVNDSANFRPQFIQFNRCENILMDGPTITNSPFWTIHPFLSKNVVLRNLKVYAHGHN
                     NDGVDPEMSENVLIENCVFDQGDDAIAIKSGCGKAAWLLNTPSKNIVIRNCTVKNGHQ
                     LVAIGSELSGGVENVFVDKCTVVDGAKLNHLLFIKTNERRGGYVKNIYMSNIVAGKID
                     QGILGIETDVLYQWRDLVPTIERSLTPIKNVYLENVKATEAKFISRVLGQKELPVENV
                     FLKNVHADLIKGNLSIHENLLNFESQN"
     gene            1006..2406
                     /locus_tag="PLQ70_11675"
     CDS             1006..2406
                     /locus_tag="PLQ70_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HQE35320.1"
                     /translation="MSSNKIIQHSEAATIFNKDVERVNWHDETLWFVREKRDKAAHQM
                     PEWELLRETASKIKNNVLSNINNYLIEFEANALKNGIIVHWAADGEEHNKIVHSIMQK
                     HGVKQMVKSKSMLTEECHLNEYLAQNGIDVIDTDLGERIVQLRNEPPSHIVLPAIHLK
                     KQDVSETFNEHLGTEKGNNDPQYLTEAARLHLRDKFLTRKVALTGVNFAIAETGEIVV
                     CTNEGNADMGVHLADVHIACMGFEKIIPQRKHLGVFLRLLARSATGQPITTFSSHFKK
                     PRAGAEMHIVIVDNGRSTQLGRPDFRNSLKCIRCGACMNTCPVYRRSGGHSYHTAVAG
                     PIGSILAPNLDMAKNADLPFASTLCGSCTNVCPVKIDIHDQLYKWRQVLVKAGHTPTV
                     KTIAMQGMASVLSSPKVFEMAGKAGRFVIKNAPFMVNNGLNAWYKQREMPAPPEESFR
                     EWYKKNGKEFKDKNNE"
BASE COUNT          824 a          440 c          521 g          627 t
ORIGIN      
        1 cattacgggt gaaggcgaac taaaagccaa aatggaagtt tggaaaggat ggtttaaaag
       61 acccaaagga catatggaaa gcttgaagcg tttgtactat ttggcttcgt acaacaaact
      121 aatgagtgag cgattaatgg tcaatgacag cgcgaatttt cgccctcaat tcatacaatt
      181 taaccgttgc gaaaacattt tgatggacgg ccctaccatt acgaacagcc ctttttggac
      241 gattcatcct tttctatcta aaaatgtagt gcttcgaaat ctgaaagttt atgcccacgg
      301 tcacaacaat gacggggtcg atccagaaat gagcgaaaat gtattgattg aaaattgcgt
      361 ttttgaccaa ggggacgatg ccattgccat taaatcgggt tgcggtaaag ctgcttggtt
      421 gttaaataca ccgtcaaaaa acattgttat ccgaaactgt acagtcaaaa acggacatca
      481 attggtagcc attggaagcg aattgtcggg tggagtcgaa aatgtatttg tagataaatg
      541 cactgttgtg gatggcgcca aattgaatca tttgctgttc attaaaacca atgaacgcag
      601 gggaggatat gtgaaaaaca tttatatgag caatattgtt gcgggcaaaa tcgatcaggg
      661 aattctaggt attgaaaccg acgtgttgta ccaatggagg gatttggttc caactatcga
      721 aagaagcttg actccaatta aaaatgtata tctcgagaac gtgaaagcta ccgaggcaaa
      781 atttatttcg agagttttgg gtcaaaaaga attgcctgta gaaaatgtat ttctcaagaa
      841 tgttcacgcc gatttgatta aaggaaatct tagcattcac gaaaatctct tgaattttga
      901 gagccaaaat tgataaataa cagcattaaa aaatgcaaaa aaaacataat ctaaaattga
      961 ttagctaagc gtttctaagt tttcaatttt taaaccaata aacagatgtc ttcgaataaa
     1021 ataattcaac acagtgaagc ggctactatt tttaacaaag atgtagaacg cgtcaattgg
     1081 catgatgaaa ccctttggtt tgttcgcgag aagcgagata aagcggcaca tcaaatgcca
     1141 gaatgggaat tgctgcgaga aactgcctcg aaaatcaaga acaacgtgct gtcgaatatc
     1201 aataattatt tgattgaatt tgaagccaat gctttgaaaa atggcatcat tgtacattgg
     1261 gctgccgatg gagaagaaca caataaaatc gtgcattcca ttatgcaaaa gcacggcgtg
     1321 aagcaaatgg taaaatccaa atcgatgctc accgaagaat gccatctcaa tgaatatttg
     1381 gcccaaaacg gaattgatgt gattgacacc gatttagggg aacgcatcgt ccaacttcga
     1441 aatgaaccac caagtcatat cgttttgcct gccattcacc ttaaaaaaca agatgtaagc
     1501 gaaactttta acgaacatct tggaactgaa aaaggaaaca acgatccaca gtatttaacc
     1561 gaagccgcac gtttgcacct cagagataaa tttctcactc gaaaagtagc tttgacggga
     1621 gtcaattttg ccatagccga aacaggtgaa attgtggttt gcaccaatga aggaaatgcc
     1681 gatatgggcg ttcaccttgc tgacgtacat attgcctgta tgggtttcga aaaaataatt
     1741 ccacagcgca aacatttagg agtttttctt cgtttactgg ctagaagcgc aacaggacaa
     1801 ccaataacca ctttttctag ccatttcaaa aaaccaagag caggagccga aatgcacatc
     1861 gttattgttg acaatggcag gagcacccaa ttaggaagac ccgattttag gaattctttg
     1921 aaatgcatcc gttgtggtgc ttgtatgaat acttgtccag tttacagacg aagtggcgga
     1981 cacagttatc acacagcagt agcgggacca attggatcta ttttggcacc aaatctcgat
     2041 atggctaaaa atgccgattt gccttttgcc agtacgcttt gcggttcttg cacgaatgtt
     2101 tgtccagtaa aaattgacat tcacgaccaa ttgtacaaat ggcgtcaagt gttggtcaaa
     2161 gcaggacata cgccaactgt aaaaaccatt gcaatgcaag gaatggcaag cgttttgtcc
     2221 agtccaaaag tgtttgaaat ggcaggaaaa gcgggaagat ttgtgattaa aaacgctcct
     2281 tttatggtga ataacggttt gaatgcttgg tacaagcaaa gagaaatgcc agcgccacca
     2341 gaagaatcct ttagagaatg gtacaaaaaa aacggaaaag aatttaaaga caaaaacaat
     2401 gagtagcaga gc
//
LOCUS       DAOSNN010000307         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_43961_length_2405_cov_4.169362, whole genome shotgun sequence.
ACCESSION   DAOSNN010000307 DAOSNN010000000
VERSION     DAOSNN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43961_length_2405_cov_4.169362"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLQ70_11680"
     CDS             <1..204
                     /locus_tag="PLQ70_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HQE35321.1"
                     /translation="KKVLVEKGIITQNGKSGKRGIRVYPPWDIPQNKQAEKTQNWQIN
                     YFLNIKTDGSTDIDLAKNLLAAN"
     gene            307..>2405
                     /gene="pheT"
                     /locus_tag="PLQ70_11685"
     CDS             307..>2405
                     /gene="pheT"
                     /locus_tag="PLQ70_11685"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQE35322.1"
                     /translation="MKISYNWLKQFIKIDWKSEETAALLTDLGLEVEVVEKYQSVKGG
                     LEGIVVGHVLTCVPHPDADRLKVTTVDLGNGIPVQIVCGASNVAAGQKVPVATIGTTL
                     YDKEGVAFQIKKGKIRGQESHGMICAEDELGLGESHEGIMILDNSIKAGTTAASVFKI
                     ENDEVFEIGLTPNRADAMSHFGTARDLRAGLLQSGVKVELISPSVSNFRIDKRILKID
                     VSVEKPLLAPRYCGVTISGITVKPSPAWLQNRLKSIGLNPKNNIVDVTNYVLHELGQP
                     LHAFDASKIHGKVIVKTLPSGTKFTTLDDVERSLHEEDLMICDEKGPLCIAGVFGGKD
                     SGVSDMTTSIFLESAYFNPVSIRKSAKRHQLNTDASFRFERGIDPTITEYALKRAALL
                     IQEVAGGEITSDIVDIYPKKIADFPVVLNFNNVKKIIGQELSKEIIKNILASLEIKVN
                     SVSDAGLGLTIPAYRVDVQREIDVIEEILRVYGYNNIKFSDKLNATVSNAPRTEDYKV
                     QNVVASQLNSQGFHEMMANSLTTSSYVQLTEMLKEEHNVLMLNPLSNDLGAMRQSLLF
                     SGLEAISYNINRRNSDLKFFEFGKTYHKFPAGYEEQKHLTMFITGNRNQENWTNAQKP
                     TDFFLFKGYVNAVLERLGIKKTQSVPVTSDVFSEGIAIGLGNDILVEYGVVKKSILKH
                     FDIKQEVLFADFNWALI"
BASE COUNT          821 a          402 c          465 g          717 t
ORIGIN      
        1 aaaaaagtgc tggtcgaaaa aggaatcatt actcaaaacg gcaaatcagg gaaacgtggc
       61 attcgagttt atccgccttg ggacattccg caaaacaagc aagccgagaa aacccaaaat
      121 tggcaaatta attatttttt aaacattaaa acggatggtt caacggatat tgatttggct
      181 aagaatctat tagctgcaaa ctagaaaatt ccattttgaa taggtaaatt ctattcaaaa
      241 aaccaaaaaa attgtttctt actccaatct gcaatcctta tatttgcatc tcattaaaat
      301 tctcaaatga aaatatcata caactggtta aaacaattca taaaaataga ctggaaatct
      361 gaagaaactg cggcattatt gaccgattta ggtcttgagg tagaagtagt cgaaaaatat
      421 caatccgtaa aaggaggttt agaaggtatt gtcgtaggtc acgttcttac ttgtgttccg
      481 catccagacg ccgatcgttt gaaagtaacc accgttgatt taggaaatgg cattccagtt
      541 caaattgttt gtggcgcatc aaatgttgct gcgggtcaaa aagtacctgt ggccaccatt
      601 ggaactactt tatatgacaa agaaggcgtt gcctttcaaa tcaaaaaagg aaaaattaga
      661 ggtcaagaaa gtcacggtat gatttgtgca gaagatgaat taggtctagg cgaaagtcac
      721 gaaggcatta tgattcttga caattccatc aaagcgggaa ctacagctgc tagcgttttc
      781 aaaatcgaaa atgatgaagt tttcgaaatt ggattgactc ccaatcgtgc cgatgcgatg
      841 agccattttg gaacagctag agatttaaga gctggtttat tgcaatctgg agtcaaagtt
      901 gaattaattt ctccatctgt gagtaatttt agaattgaca aaagaattct taaaattgat
      961 gttagtgtcg aaaaaccact tttagcacca agatattgcg gtgtaactat ttctggaata
     1021 accgtaaaac catcacccgc ttggttacaa aaccgattga aatctattgg attaaatcca
     1081 aaaaataata ttgtagatgt tactaattat gttttacacg aattaggaca accacttcac
     1141 gcttttgatg cttccaaaat tcacggcaaa gtaattgtaa aaacacttcc ttcaggaacc
     1201 aaatttacaa ccttagacga tgtagaaaga agtttgcatg aagaagattt aatgatttgt
     1261 gatgaaaaag ggcctttatg tattgctggt gtttttggag gaaaagattc gggagtttca
     1321 gatatgacca cttctatttt cttagaaagt gcctatttta atcctgtaag cattagaaaa
     1381 agcgccaaaa gacatcaatt aaataccgat gcttctttcc gttttgaaag aggaattgat
     1441 ccaaccatca cggaatatgc cttgaaacgc gccgccttgt tgattcaaga agtagctggc
     1501 ggtgaaatca cttcggatat tgttgatatt tatccaaaga aaattgctga ttttccggtg
     1561 gttttaaatt tcaataatgt taagaaaatt attggtcaag aattatctaa agaaatcatt
     1621 aaaaacattt tggcttcgct ggaaatcaag gtaaacagcg tttcggatgc cggattggga
     1681 ttgaccattc ctgcttatcg cgtggacgtc caaagagaaa tcgacgttat cgaggaaatt
     1741 cttcgagtgt acggttacaa caacatcaaa ttttcggaca aattgaatgc cacagtttct
     1801 aatgcgccaa gaaccgaaga ttataaagtg caaaacgttg ttgcttctca actgaattca
     1861 caaggttttc acgaaatgat ggccaattca ttaacgactt cttcctacgt tcaattgact
     1921 gaaatgctca aagaagaaca taatgtattg atgttaaatc cattgagtaa tgatttggga
     1981 gctatgcgtc agtctttgtt gttttcagga ttggaagcca tctcttataa tatcaatcgc
     2041 agaaattctg atttgaaatt ctttgaattt ggaaaaacct accataaatt tccagcaggg
     2101 tatgaagaac agaaacactt aacaatgttc atcactggaa accgaaatca agaaaactgg
     2161 actaatgccc aaaaacctac tgatttcttc ttgttcaaag ggtatgtaaa tgctgttttg
     2221 gaacgattgg gaatcaaaaa aacacaaagc gttcctgtta catccgatgt tttctctgaa
     2281 ggaattgcta tcggtttagg caatgacatt ttggtagaat atggtgtggt aaaaaaatca
     2341 atattaaaac atttcgacat caaacaagaa gttttgtttg ctgatttcaa ttgggcattg
     2401 atttt
//
LOCUS       DAOSNN010000308         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_44069_length_2401_cov_5.081841, whole genome shotgun sequence.
ACCESSION   DAOSNN010000308 DAOSNN010000000
VERSION     DAOSNN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44069_length_2401_cov_5.081841"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLQ70_11690"
     CDS             complement(<1..713)
                     /locus_tag="PLQ70_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQE35323.1"
                     /translation="MKRLFYILLLFSTVINAQETVSRELSYNEFLGYVKKNHPLVKNA
                     QLELNKAQAELMMARGSFDPKLEANYDQKQFKGKEYYSILNSSFKIPTWYGVEIKAGF
                     DDNEGYYLNPQNTTPAQGLTSLGISVPLGQGLWINKRMADLRKAKMQIQLSQAEVKLE
                     AIAVLYDASLAYFNWEKNYEEVQLYENYSRNAQIRNKGIQSLIEQGDKPAIDSIEAGI
                     IVKNRLLSLEDSKLKLAKA"
     gene            complement(713..2059)
                     /locus_tag="PLQ70_11695"
     CDS             complement(713..2059)
                     /locus_tag="PLQ70_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020211188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HQE35324.1"
                     /translation="MLNISENNKIKQSFTDFKTIYTIENKTHYKILNKIIIGVSIFSF
                     CLLFLPWTQNISGSGAVTTLKPNQRPQSIQSVISGRIEKWYVQEGDFVQKGDTILYIS
                     EIKEDYMDPNLVENTKNQVEAKKQATQSYASKVGSLDDQLRAIEAEKRLKLEQAQNKI
                     KQARLKIKSDSIDLIAVNTQLKIANTQYNRALQLNKEGLKPLTDLEEKRLKLQDVEAK
                     IINQENKLLSSKNEYINSKVEINRIMAEYSEKAAKSESDQFTARSSQYDAAAQVNKLE
                     NQYVNYSIRNKMYYIRAAQSGYINRALQSGIGETIKEGTAIATIMPAEYDIAVETFVN
                     PVDLPLINKGEKVRIWFDGWPTIVFSGWPDVSYGTFGGEIVAIENFISDNGKYRVLIA
                     PDKNEAKWPKQLSIGSGAQTLALLDTVPIWFEIWRTLNGFPPNYYKKDTKPSKEKK"
     gene            complement(2064..>2401)
                     /locus_tag="PLQ70_11700"
     CDS             complement(2064..>2401)
                     /locus_tag="PLQ70_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQE35325.1"
                     /translation="WAINGVQLVSFIKSLPEGLDTIVFPEGKQLSSSNIQKILLARSI
                     IHKPRILFYEDPTDKMDNDVSNEIIDFITAKEHLWTIIVSSKNPYWKDKSNRIITMQN
                     GKIVLDSKI"
BASE COUNT          633 a          440 c          425 g          903 t
ORIGIN      
        1 ttggctttgg ctaatttaag tttagaatct tctaaactca aaagtctgtt ttttacaata
       61 attcctgctt caatactgtc gatggcaggt ttgtcgcctt gttcaatcaa agactgaatt
      121 cctttatttc ttatttgagc atttcgactg tagttttcat acaattgaac ttcttcatag
      181 tttttttccc aattgaaata cgctaaagag gcgtcgtata aaaccgcaat cgcttcgagt
      241 ttcacttcgg cttggctcaa ttgaatttgc atttttgctt tgcgcaaatc ggccattctt
      301 ttattaatcc aaagtccttg tcctaaagga acgctaattc ctaaagaagt caacccttgg
      361 gcaggcgtgg tgttttgcgg attcaaataa taaccttcgt tgtcgtcaaa accagcttta
      421 atttcgacgc cataccaagt tggaattttg aaactactgt tcaaaataga ataatattct
      481 ttgcctttga attgtttttg gtcgtaattg gcttctagtt ttgggtcgaa actacctctt
      541 gccatcatca attccgcttg ggctttgttg agttccaatt gggcattttt taccaaagga
      601 tgattttttt tcacataccc caaaaactca ttataactca attctcttga gaccgtttct
      661 tgtgcattta taactgtcga aaacaataac aatatataga aaagtcgttt cattatttct
      721 tttctttaga aggttttgtg tcttttttgt aataattagg aggaaatccg tttagggtcc
      781 tccaaatttc aaaccaaatc ggaacagtgt ctagcaaagc caatgtttgt gcacctgaac
      841 caatgcttaa ttgtttcggc cattttgcct catttttatc tggggcaatc aatacacggt
      901 attttccgtt gtcgctaatg aagttttcga tggcaacaat ttcgccacca aaagtaccat
      961 acgaaacatc aggccaaccc gaaaaaacaa ttgttggcca tccgtcaaac caaattcgca
     1021 ctttttcgcc tttgttaatt aatggtaaat caacaggatt gacgaatgtt tcaacagcga
     1081 tatcgtactc ggcaggcatt atggttgcaa tggctgttcc ttccttaatg gtttcgccaa
     1141 ttccggattg caaagcccta ttgatgtaac cactttgtgc cgctctgatg tagtacatct
     1201 tgttgcgaat gctgtaattg acatattgat tttcgagttt atttacttgt gcggcagcat
     1261 catattgact gcttcgggcg gtaaattggt cgctctcgga tttggctgct ttttctgaat
     1321 attcggccat aattcggtta atttcaactt ttgaattgat gtattcattt ttgctgctca
     1381 ataatttatt ttcttgattg ataattttgg cttcaacgtc ttgtagtttc aagcgttttt
     1441 cttcgaggtc agtcaagggt tttaagcctt ctttgttcaa ttgcaaagca cgattgtatt
     1501 gagtattggc aatttttaac tgggtgttga ccgcaatcaa atcgatactg tcgcttttaa
     1561 ttttcaagcg ggcttgtttg attttgtttt gagcttgttc taatttgagt cttttttcag
     1621 cttcaatcgc tcgcaattga tcgtctaaac tccctacttt tgaggcatag gattgtgtgg
     1681 cttgtttttt ggcttccact tggtttttgg tgttttcaac caaattcgga tccatataat
     1741 cctcttttat ttcagaaata taaagaatgg tatcgccttt ttgtacaaaa tcgccttctt
     1801 gtacatacca tttttctatt cgacctgaaa tgacactttg tatcgattgt ggtctttggt
     1861 tgggttttag cgtagttacc gctcccgaac ccgaaatgtt ttgggtccac ggaagaaaca
     1921 aaaggcaaaa actgaatatg gaaacaccga ttataatttt atttaaaatt ttataatggg
     1981 ttttgttttc aatcgtatat atggttttga aatcagtaaa cgattgctta attttattat
     2041 tttcagatat atttagcatc ttcttaaatt ttggagtcta gtacaatttt accgttttgc
     2101 attgtgatga tacgattgct tttgtctttc caataagggt ttttagacga aacaataata
     2161 gtccataaat gttctttggc ggttataaaa tcaataattt cattcgaaac atcattatcc
     2221 attttgtctg ttggatcttc gtaaaaaaga attctaggtt tgtgaatgat acttctcgct
     2281 aataatattt tctgaatatt agaggaggac aattgctttc cttcaggaaa aacaatcgta
     2341 tctaatcctt cgggtaatga tttgataaaa cttaccaatt gcacgccatt aattgcccat
     2401 t
//
LOCUS       DAOSNN010000309         2391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_44321_length_2391_cov_4.142123, whole genome shotgun sequence.
ACCESSION   DAOSNN010000309 DAOSNN010000000
VERSION     DAOSNN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44321_length_2391_cov_4.142123"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2005)
                     /locus_tag="PLQ70_11705"
     CDS             complement(<1..2005)
                     /locus_tag="PLQ70_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HQE35326.1"
                     /translation="MEVFLFVLLIAFLFYIKNAIDSKFDRFEDRIEDKIDEKFKELFK
                     KIEASKGTEKLPEKPIVAPEIAKPKQTPETIKPTVVIEKTEPLVFNPIEKEVAKEKIV
                     FSMDNASAPKHKQPQAEPKIHIPNEPTKTFWENFRDNNPDLEKFIGENLINKIGILIL
                     VLGISYFVKYAIDKDWINEPARVGIGMLSGGLIMGIAHKLRQKYAAFSSVFVAGAIAV
                     FYFTIGIAFHSYHLFGQTTAFIIMVLITVFSSLISLSYNRKELAVLSLIGGFAVPFMV
                     STGQGNYVVLFTYILILNIGILALAYYKKWGIINILSYVFTVILYGAWLFKDLDEKTP
                     HYLGALAFGFAFYFVFILINIINNVRTKGLFSPIELSILASNTFLFYGAGMAILEQFH
                     PEFKGLFTTLLGVLNFGYAYFLYKKFGLDKTAVYLLIGLTLTFLTLAIPIQFEGNFIT
                     LFWAAEAVMLMWLAQKSKITYYRFASVIVHLLMIGSLLMDWSAIYMSSTILTIVFNKI
                     CLTGIFAVGSLFAVYYLLKKEDEDLEQFGFIFNPEKYRNVLGLIGIILTYFVGLFEVN
                     FQANAHIQGANSALTLPILYHLIFCALSVYFLCKIKNTLGYQLASIIAVVNIILFVFF
                     FLNYAFYEHEYYITTGNHQRIGLYLHYISLLITLFFGYKLYQIN"
BASE COUNT          830 a          377 c          427 g          757 t
ORIGIN      
        1 tgttgatttg gtataattta tagccgaaaa acaaggttat aagtagtgaa atataatgca
       61 aatataaccc aattctttga tgatttccag ttgtaatgta gtactcgtgc tcataaaaag
      121 cataattcaa aaagaaaaac acaaagagta ttatattgac tacggctata atggatgcta
      181 attgataacc aagagtgttt ttgattttgc acaagaagta cacagacaaa gcacagaaaa
      241 ttaaatgata taaaataggt aatgtcaagg ctgaattggc tccttggata tgtgcgttgg
      301 cttgaaaatt cacctcaaac aatccaacaa aataggtgag gattattcca atcaaaccta
      361 gaacgtttcg gtatttttct ggattgaaaa tgaatccaaa ttgttccaaa tcttcgtctt
      421 cttttttcaa taaatagtaa acggcaaata aagaacccac ggcaaaaata cctgttaggc
      481 atattttgtt gaagacaatg gttaaaatag tggatgacat ataaatagca gaccaatcca
      541 tcaataaact tccaatcatc aacagatgaa cgatcaccga ggcaaatcga taataagtta
      601 tttttgattt ttgtgccagc cacatgagca tcacggcttc ggcagcccag aaaagggtga
      661 tgaaattccc ttcgaattgt attggaatgg ccaatgttaa gaaggtaagt gtcaatccaa
      721 tcaacaagta aacggcagtt ttgtccaagc cgaatttttt gtataaaaaa taagcgtatc
      781 caaagtttaa aactcctaat aaagtagtga ataatccttt gaattcggga tgaaattgtt
      841 ctaaaatagc cattccagcc ccataaaaaa gaaaggtatt gctagctaga atactcaatt
      901 ctataggaga gaaaagacct tttgttcgaa cattattgat gatgttgatg aggatgaaaa
      961 caaaataaaa ggcaaatccg aaggctaatg cacccagata atgcggtgtt ttttcgtcaa
     1021 ggtctttaaa gagccaagct ccatacaaaa tcactgtaaa aacgtaggac aaaatattga
     1081 taatccccca ttttttataa taggccaatg ccaaaatccc gatgttcaag atgagaatgt
     1141 aagtaaacaa aacgacataa tttccttgtc cagtgctgac cataaacgga acggcaaacc
     1201 cgccaatcag ggataataca gctagttctt tgcgattgta agaaagtgaa attaggctac
     1261 tgaaaacggt gataagcacc ataataataa aggctgttgt ttgtccaaac aaatggtagc
     1321 tgtggaaagc aatgccaatt gtaaaataga aaactgcaat ggcacccgcc acaaaaaccg
     1381 aactgaaagc agcatatttt tggcgcaatt tatgtgcaat tcccataatc aatccgccag
     1441 atagcatgcc aattcccaca cgtgctggtt cgttgatcca gtctttgtca atggcatatt
     1501 tcacgaaata actgattccc aaaaccagaa tcaaaatacc aattttgttg atgaggtttt
     1561 caccaataaa cttttctaaa tcgggattgt tatccctgaa attttcccaa aaggttttag
     1621 taggttcatt agggatgtga atttttggct cggcttgtgg ttgtttgtgt tttggagctg
     1681 aggcattgtc cattgaaaag acaattttct ctttcgcaac ctctttttca attgggttaa
     1741 aaactagagg ttctgttttt tcaatgacca cagttggttt tattgtttct ggagtttgtt
     1801 ttggttttgc aatttcagga gcaactatag gtttttctgg aagtttttcg gttccttttg
     1861 atgcctcaat ttttttaaat aattctttga atttctcatc gattttgtct tcaattcgat
     1921 cttcgaatct gtcgaatttt gagtcgatag catttttgat gtagaataaa aaagcaatca
     1981 ataatacaaa taagaaaact tccatagcga tgttttgaaa attaaataat gtaaatataa
     2041 tgataaatac aaaacagcgt gctatttttc tgaatctaaa gttaagagtg tttgggatat
     2101 ttgtatgtcc tagtagaatt tttagtgtgt tatttttaaa ctattttaaa tttgattttt
     2161 gcagaactaa aaacaaggtg tataaaaaaa actgagatct attaaaaaaa tagacctcag
     2221 ttaagtttga tgttggtaga aaattatttc tttatgaatt tccaagttgt tgatgattct
     2281 cctattttaa gattacataa atacaatcca tttgataagt tggcaacatt tatggaaagt
     2341 ttttcagaag gagaaactat tttggaatta gaatatactt tttgtcccga a
//
LOCUS       DAOSNN010000310         2366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_45001_length_2366_cov_3.125920, whole genome shotgun sequence.
ACCESSION   DAOSNN010000310 DAOSNN010000000
VERSION     DAOSNN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45001_length_2366_cov_3.125920"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="ruvB"
                     /locus_tag="PLQ70_11710"
     CDS             <1..963
                     /gene="ruvB"
                     /locus_tag="PLQ70_11710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017498167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQE35327.1"
                     /translation="KRLRPLSFDDFAGQDQILENLKVFVQAANQRNEALDHALFHGPP
                     GLGKTTLANILANELQVGIKITSGPVLDKPGDLAGLLTNLEERDVLFIDEIHRLSPIV
                     EEYLYSAMEDFKIDIMIESGPNARTVQINLNPFTLIGATTRSGLLTAPMRARFGISSR
                     LQYYTTELLTTIVERSASILKMPISMEAAIEIAGRSRGTPRIANALLRRVRDFAQIKG
                     NGTIDIEIARYALKALNVDAHGLDEMDNKILNTIIDKFKGGPVGLSTLATAVSESSET
                     IEEVYEPFLIQEGFIMRTPRGREVTEKAYKHLGKVRTNVQGGLF"
     gene            966..1892
                     /gene="queG"
                     /locus_tag="PLQ70_11715"
     CDS             966..1892
                     /gene="queG"
                     /locus_tag="PLQ70_11715"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQE35328.1"
                     /translation="MINNKEKYAQFIKAESKRLGFLSCGISKAGFLEEEAPRLERWLK
                     NQMNGQMFYMENHFDKRLNPTLLVDDAKSVVSLLLNYYPSEFQNEESYKISKYAYGQD
                     YHFVIKDKLKELLFSIQSNIGEVSGRAFVDSAPVLDKAWAAKSGLGWIGKNSNLLTQK
                     LGSFYFIAELIIDLDLEYDHATTDHCGSCTACIDACPTNAIVAPYVVDGSKCISYFTI
                     ELKENIPQEMKGKFDNWAFGCDVCQDVCPWNRFSKAHNEPLFTPNKEMLSMSKKDWTE
                     ITENTFNLVFKNSPVKRTKYSGIRRNIDFLKE"
     gene            2007..>2366
                     /locus_tag="PLQ70_11720"
     CDS             2007..>2366
                     /locus_tag="PLQ70_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQE35329.1"
                     /translation="MTVNQILAVKGKEVYSVLPSITVYEALVSMSEKNIGAILVVEDN
                     VLKGILSERDYARKIALKAKSSKKTFVHEIMASNVITVKPSDNLEYCMELMYTKKIRH
                     LPVLENENIIGIISIGDV"
BASE COUNT          781 a          368 c          463 g          754 t
ORIGIN      
        1 aaaagattaa gacccttgtc ttttgatgat tttgcaggtc aggaccaaat actcgaaaac
       61 ttgaaggttt ttgttcaagc ggccaatcaa cgaaatgaag cccttgatca tgcgctattt
      121 cacggccctc caggattagg aaaaactact ttggctaata ttctcgccaa tgaattgcaa
      181 gttggaatca aaattacttc tggacctgtt ttagataaac caggtgattt ggcaggttta
      241 ttgaccaatc tcgaagaaag agatgttttg tttattgatg aaattcaccg tttgagtccc
      301 attgtcgaag aatatttata ttcggctatg gaagatttca agattgatat tatgattgaa
      361 tcagggccaa atgctagaac cgttcaaatc aatttgaatc cgtttacatt aattggtgct
      421 acaactcgtt ctggattatt gaccgctcca atgcgagctc gttttgggat ttcatcgcga
      481 ctacagtatt acaccaccga acttttaaca acaatagtcg aaagaagtgc atccatcctt
      541 aaaatgccta tttctatgga agccgctatc gaaattgcag gtcgaagtag aggaacgcct
      601 cgtatagcca atgccttatt gcgaagagtg cgtgattttg ctcaaatcaa aggaaatgga
      661 accatcgaca tagaaattgc tagatatgct ttaaaagcct taaatgttga cgctcacggt
      721 ttggacgaaa tggataataa aatcctaaac accattatcg ataaattcaa aggaggacct
      781 gtaggattat ccactttggc aacagccgta tctgaaagta gcgaaactat cgaagaagtg
      841 tacgaaccct ttttaattca agaaggtttc attatgcgaa caccaagagg aagagaagta
      901 actgagaaag cttataaaca tttggggaag gttagaacga atgttcaagg cggattattt
      961 taattttgat aaacaataaa gaaaaatacg cgcaatttat aaaagccgaa tctaaaagac
     1021 tcggcttttt atcttgtggc atatccaaag cagggttttt ggaagaagaa gcaccccgat
     1081 tggaacgttg gttaaagaat caaatgaacg gtcaaatgtt ctatatggag aatcattttg
     1141 acaaacgttt gaatccaact ttgttggttg atgatgccaa aagtgtggtt tcgcttttgt
     1201 tgaattatta tccttccgaa tttcagaatg aagaaagcta taaaatttca aaatatgcct
     1261 acggtcaaga ttaccatttt gtcattaaag acaaattgaa agaattgttg ttttcgattc
     1321 aatctaatat aggtgaagtt tcggggcgtg cttttgtaga ttcggctccc gttttggata
     1381 aagcttgggc agccaaaagc ggtttgggtt ggattggtaa aaacagcaat ttattaaccc
     1441 aaaaactagg ttcgttttat ttcattgccg aattgattat cgatttagat ttagaatacg
     1501 accatgccac aaccgatcat tgtggctctt gcaccgcttg tattgatgct tgtccaacca
     1561 atgctattgt tgcgccttat gtggttgacg gtagtaaatg tatttcctat tttaccatcg
     1621 aactcaaaga aaacattcca caagaaatga aaggtaaatt tgataattgg gcatttggtt
     1681 gcgatgtttg tcaagatgtt tgtccttgga atcgcttttc gaaagctcat aacgaacctc
     1741 tttttactcc gaataaagaa atgctttcga tgtctaaaaa ggattggaca gaaatcactg
     1801 aaaatacttt taatttggtt tttaaaaatt ctcctgtcaa aaggaccaaa tattctggaa
     1861 ttaggcgaaa tatagatttc ctaaaagagt gatttttaag ttaaaattgt actgtgtttt
     1921 ataatttttt gttttaaata tttgattagg gtgttgtaaa attagttttt ttttctaatt
     1981 ttataattca attaaaattt tctattatga cagtaaatca aatattagca gtaaaaggga
     2041 aagaagtata ttcagtattg ccttcaatca cagtatatga agctttggtg agtatgagcg
     2101 aaaaaaatat tggagctatt ttagtagtag aagacaatgt tttaaaagga atcttatccg
     2161 aaagagatta tgctcgaaaa attgctttga aagctaaatc ctcgaaaaaa acatttgtac
     2221 acgaaattat ggctagcaat gttataacgg taaaaccttc cgacaatttg gagtattgta
     2281 tggaattaat gtacaccaaa aagatcaggc atttacctgt tttagaaaat gagaatatta
     2341 ttggaattat ttctattgga gacgtt
//
LOCUS       DAOSNN010000311         2293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_46931_length_2293_cov_5.339589, whole genome shotgun sequence.
ACCESSION   DAOSNN010000311 DAOSNN010000000
VERSION     DAOSNN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46931_length_2293_cov_5.339589"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLQ70_11725"
     CDS             complement(<1..285)
                     /locus_tag="PLQ70_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HQE35330.1"
                     /translation="MFKKIIFVFSLLILLNSCASQQKKFSKSDWYAFTKTSSERVEPS
                     KTYEFTDGMIRMHGENIGYLMSKKSYKNFELSLDFRWNIDPKFSRGNVKKN"
     gene            complement(317..>2293)
                     /locus_tag="PLQ70_11730"
     CDS             complement(317..>2293)
                     /locus_tag="PLQ70_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQE35331.1"
                     /translation="KSITKTESQKFYLDSIKKTFVKDELASCVDSLWLKELTNLDLFN
                     DISDDIKNINIDEKVDYELPTELLKQRLAAMDAKSPFNIEYNPGLENIIKSFLKNRKK
                     SFGRLMAISEYYFPMFEEALAKQNVPLEIKYLAVVESALNPKAVSRVGATGLWQFMYQ
                     TGKQYNLKIDSYVDERSDPLKSSEAAAQYMSKMYDIFGDWDLVLASYNSGPGNVAKAI
                     RRSGGQQNYWNIRKNLPKETQGYVPAFLATMYIYEYHKEHGIKPERALVQNFATDTIL
                     IKKQMSFKQIADLLDVSVSQLQLLNPSYKMNVIPYYGDQDHFLRLPKDKIAVFASNED
                     KIYAYVQQDLDFREKPFQVTKAIVLNDTLNATQRVSLPKTKYYTVKSGDNLTEIANKY
                     SVTVNDIKKWNSLKGSTVARGKSLKIITNESVAKTVKNEPKTNNGPLEVKTNQQVASS
                     ESKVNKDDKTNKNIKSDTLSTAVASYYVVQKGDNLSSIAKKNNVSVAEIKDWNNLSTN
                     SIQLGASLQVGKIEGNEKEQIALTTKTENVEYIVKKGDNLGNISKKFGSTIDDLKQWN
                     NLQDNAIALGSSLIVAKSEVPLENKAVAETLTKKPNLASSSKTNITNYLVQKGDSLYS
                     ISKKYPGVSISDIKKWNNISGEELKPGMKLKING"
BASE COUNT          654 a          388 c          351 g          900 t
ORIGIN      
        1 attctttttt acattccctc tactaaattt tgggtctata ttccatctaa aatcaagact
       61 aagttcaaag tttttataac tttttttgct cattaaatag cctatatttt caccgtgcat
      121 tcgaatcatg ccatcggtaa actcatatgt ttttgaaggt tctactcttt cggaactggt
      181 tttagtaaaa gcataccaat cgctcttgga gaattttttt tgctgagaag cacaagaatt
      241 taataaaata agtaatgaaa aaacgaaaat tatttttttg aacattgggg taattgtata
      301 actttaaaat gaagttttaa ccgttaattt ttagtttcat tccgggtttt aattcttcgc
      361 cgctgatgtt gttccatttt ttaatatctg aaatactaac tccaggatat ttttttgaaa
      421 tactatacaa agaatctcct ttttgtacta aataattggt gatattggtt tttgaagatg
      481 aagctaaatt tggtttttta gtcaatgttt cagctacagc tttgttctct agcggtactt
      541 cgcttttagc aacaatcaat gaacttccca acgcaatggc attgtcttgc aaattattcc
      601 attgtttcaa atcgtctatt gtgcttccga attttttgga aatatttcca agattatctc
      661 ctttttttac aatatattca acattttctg tcttggttgt caaagcaatc tgttcctttt
      721 catttccttc gattttacct acttgcaatg atgctcccaa ttgaattgaa ttagtagaaa
      781 gattattcca atccttgatt tcggctacag aaacattgtt ctttttggcg atactgctta
      841 aattatctcc tttttgaacc acataataag aggcaacagc cgttgataaa gtatccgatt
      901 ttatattttt attcgtttta tcgtctttat taactttgga ttctgaactg gcaacttgtt
      961 gatttgtttt aacttctaaa ggaccattgt tggtttttgg ttcattttta accgtttttg
     1021 caacgctttc attagtaatt atttttaaac tttttccgcg tgcaactgta cttcctttta
     1081 agctattcca ctttttaata tcatttactg taacgctata tttattagca atttcagtta
     1141 gattatcccc gctttttaca gtataatatt ttgttttagg aagtgatact cgttgcgtag
     1201 catttaaagt gtcattcaag acaatggctt ttgtaacttg aaatggtttt tcacgaaaat
     1261 ctaaatcctg ttgtacatag gcataaatct tgtcttcgtt tgaggcgaaa acggcaattt
     1321 tatcctttgg taatcttaaa aaatgatctt gatctccata atacggaatc acattcattt
     1381 tataagaagg gtttaaaagt tgtagttgag acacagaaac gtctagcaaa tcagcaattt
     1441 gcttgaacga catttgcttt tttattagta tggtatcagt agcaaaattc tgtactaatg
     1501 ctctttctgg cttaattccg tgttctttgt ggtattcata aatgtacatt gttgctaaaa
     1561 aagctggtac atatccttga gtttctttgg gtaaattttt tctaatattc caataatttt
     1621 gttgtccacc cgatcggcga attgcttttg caacattgcc tggtcctgaa ttataagatg
     1681 ccaaaaccaa gtcccaatcg ccaaaaatat catacatttt gctcatatat tgggctgcag
     1741 cttcacttga ttttagtgga tcactacgtt catcaacata ggaatcaatt tttaaattgt
     1801 actgttttcc ggtttggtac ataaactgcc aaagtccggt tgctcccact cttgaaactg
     1861 cttttggatt caaagctgat tcaacaacag ccaaatattt aatttctaat ggtacatttt
     1921 gtttggccaa tgcctcttca aacataggga agtaatattc agaaatagcc atcaatcgac
     1981 caaaagattt ttttcgattc tttaaaaaag atttaataat gttttctaaa ccgggattgt
     2041 attcgatatt aaaaggagat ttggcatcca ttgccgctaa tctttgtttg agcaattcgg
     2101 tgggcaattc ataatcgact ttctcgtcga tattgatgtt ttttatatcg tcggatatat
     2161 cattaaataa atcgagattt gttaattcct tcaaccacag actgtcaaca caagaggcga
     2221 gttcatcttt tacaaaagtt tttttgatgg aatccaaata gaatttttga ctttctgttt
     2281 ttgtaatcga ttt
//
LOCUS       DAOSNN010000312         2259 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_47898_length_2259_cov_6.051270, whole genome shotgun sequence.
ACCESSION   DAOSNN010000312 DAOSNN010000000
VERSION     DAOSNN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47898_length_2259_cov_6.051270"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..222)
                     /locus_tag="PLQ70_11735"
     CDS             complement(46..222)
                     /locus_tag="PLQ70_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008254968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35332.1"
                     /translation="MTHLILPLLQVNVEEKLKNAPDNGYQIGVIIGSYLPFVILVGLA
                     YWMYYRAKKGDKKE"
     gene            complement(226..990)
                     /locus_tag="PLQ70_11740"
     CDS             complement(226..990)
                     /locus_tag="PLQ70_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HQE35333.1"
                     /translation="MLSENLNGSIITTIENKIATLEFGHPASNSFPSDLLNRLTNELN
                     ILSNNTNVSVIVLKSSGSGAFCAGASFDELLAVSNPEEATEFFSGFANVLNAMRTCSK
                     IIIGRIHGKAVGGGVGIISACDYALATTESSIKLSELTIGIGPFVIEPAVSRKIGKSA
                     TAEMALDTEWKTAAWAGQKGLYAKVLETQEKLDQEITTMANKLASYNPEALFEMKKVL
                     WEGTENWNTLLYERAEISGKLVLSDFSRKALSQLKK"
     gene            1094..1167
                     /locus_tag="PLQ70_11745"
     tRNA            1094..1167
                     /locus_tag="PLQ70_11745"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1128..1130,aa:Ala,seq:ggc)
     gene            complement(1237..>2259)
                     /locus_tag="PLQ70_11750"
     CDS             complement(1237..>2259)
                     /locus_tag="PLQ70_11750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HQE35334.1"
                     /translation="GMKKQVPLWRRGVQRVNDNLGEIVGKVYVKNHFSPEAKEKVTVL
                     VKNLLKAYGESIKNLDWMSLETKKQALDKLSKFTLKIGYPDKWRDYSSLKVVKGDLFG
                     NLQRAAVFENNRQIAKLGKPVDRTEWEMTPQTVNAYYNSTLNEIVFPAAYLQPPYFDL
                     LAEDAANYGGVGATIGHEIGHGFDDQGSTFDGDGVMRNWWTPEDLTAFKQKTAALVTQ
                     YNGFKIFPDLNVNGEFTLGENIGDLGGLTIALKAYKESLNGKESPVIDGFTGDQRVFI
                     AFGQSFLGKIREEALRSLMASDPHSPDKYRVNGVVRNIPEFYTAFNIKPTDSLYLAPD
                     KRVKIW"
BASE COUNT          617 a          443 c          397 g          802 t
ORIGIN      
        1 ttattttgct cattctattt ttatttgagt gcaaatttag tgaatctact cctttttgtc
       61 tccttttttc gcccgataat acatccaata ggctaggcca accaatataa caaaaggaag
      121 ataagaacct attatgacac caatttgata accgttgtct ggtgcgtttt ttagtttttc
      181 ttcgacattt acttgtagca aaggcaagat gaggtgtgtc attgtttatt ttttaagttg
      241 agacaaggct tttcttgaaa aatccgagag caccaatttt cccgaaattt cggctctttc
      301 atataataga gtgttccagt tttcggttcc ttcccaaagc acttttttca tttcgaataa
      361 agcttccggg ttgtaactgg ctagtttatt ggccatagtc gtaatttctt ggtctaattt
      421 ttcttgtgtt tccaatactt tggcatacaa tcctttttgg cctgcccaag cggcggtttt
      481 ccattccgtg tctaaagcca tttcggccgt ggcacttttt cctatttttc tgctcacggc
      541 aggttcaatc acgaaaggcc caattcctat ggttaattct gataatttta tggaactttc
      601 ggtagttgcc aaggcataat cacaggctga tataattcct actccgccac caacggcttt
      661 tccgtgaatt cttccaatta taatttttga acaagttcgc attgcattca agacattggc
      721 aaaacccgaa aagaattctg tggcttcttc gggattggaa actgccaaaa gttcgtcaaa
      781 agaagctccg gcacaaaagg ctccagaacc actacttttt aaaacaatta ccgaaacatt
      841 agtgttgttg cttaaaatat tcaattcgtt agttaatcga ttcagtaaat cactcggaaa
      901 agaattgctc gcaggatgcc caaattcaag ggttgcgatt ttgttttcga tagttgtgat
      961 aatagaaccg tttaaatttt ctgaaagcat tgcactgatt ttgatgtaaa gttaaaaata
     1021 ttaagacaag caggatattt tttttcaaaa tttgctttgt gctatattta tatgttatat
     1081 ttgccactcg attgggggat tagctcactt ggctagagcg cttgcctggc aggcaagagg
     1141 taaccggttc gattccggta ttctccacaa aaaaaaccat aattactttc tggagtaatt
     1201 atggttttta ttttaatgaa gttttaaata ttgaatttac cagattttaa cccttttatc
     1261 gggagctaaa tataaagaat cagtaggttt tatgttgaat gcagtgtaaa attctggaat
     1321 gtttctcacc actccattaa ccctgtattt gtcgggagaa tgagggtcgc ttgccataag
     1381 acttcgtagg gcttcttcac gaatttttcc taaaaaactt tggccaaagg caataaacac
     1441 acgttgatcc cctgtgaaac catctataac gggagattcc ttgccattaa gactttcttt
     1501 gtaggctttc aatgctatag ttagtcctcc taagtctcca atattttctc ctagtgtaaa
     1561 ttcaccattt acattgagat caggaaatat tttgaatcca ttgtattggg tcactaaagc
     1621 agctgttttt tgtttaaagg cagttaaatc ttctggcgtc caccagttgc gcatcacacc
     1681 gtcaccatca aaagtacttc cttggtcgtc aaatccatgt ccaatttcat gtccaattgt
     1741 tgcgccaact ccgccgtaat ttgctgcatc ttcagccaat aaatcaaagt agggtggttg
     1801 caaataggct gctggaaaca caatttcgtt taatgttgaa ttgtaatagg catttaccgt
     1861 ttgtggtgtc atttcccact ctgttctatc aacgggtttg cctaatttgg cgatttgtct
     1921 attgttttca aagacagcgg ctctttgtaa gttgccaaaa aggtcacctt tgactacttt
     1981 taaggaggaa taatctctcc atttgtcagg gtatccaatt tttagagtga atttagataa
     2041 tttatccaag gcttgctttt tagtttcaag actcatccaa tccaaatttt taatgctttc
     2101 gccataagct tttagaagat tttttactaa aacagttact ttttcttttg cttcaggtga
     2161 aaaatgattt ttaacatata cttttccaac aatttcacca agattatcat ttactctttg
     2221 aactcctcta cgccacagag gtacttgttt tttcattcc
//
LOCUS       DAOSNN010000313         2250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_48126_length_2250_cov_4.669704, whole genome shotgun sequence.
ACCESSION   DAOSNN010000313 DAOSNN010000000
VERSION     DAOSNN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48126_length_2250_cov_4.669704"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLQ70_11755"
     CDS             <1..311
                     /locus_tag="PLQ70_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HQE35335.1"
                     /translation="SLTYEESNRLYQEVLKDYEGETKYKQFQKVKANEYFNGLQVKFS
                     YAITCHKSQGGQWNTVFVEQPYLPDGINRDYIRWLYTAMTRAKNKLYLIGFKDESFVD
                     "
     gene            complement(308..670)
                     /locus_tag="PLQ70_11760"
     CDS             complement(308..670)
                     /locus_tag="PLQ70_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQE35336.1"
                     /translation="MENKISFKEAKACPIQFVLAVNDTLNVINGKWKLPIIGSLLFEK
                     RRFTEIQRNIPKITPRMLSKELKDLEMNGMVKRTVYDTTPITVEYELTESAKSLGDVL
                     DKMLEWGLQHRETVLSES"
     gene            790..1668
                     /locus_tag="PLQ70_11765"
     CDS             790..1668
                     /locus_tag="PLQ70_11765"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE35337.1"
                     /translation="MSAKILVTGATGNFGKTTIDFLLKKGIAATNISALVRDEAKAED
                     LKAKGITIVKGDYDNYDSLLAAFKGVDKLLLVSATDLENRSKQQLNAVKAAKEAGVKH
                     ILYTSFERKNESETSPIAFLAKSHIDTDNAIKASGMTYSIFRNNLYLEVLPMFFGEQV
                     LATGIFFPAGDTAAAYVSRNDLAEAVANVLIAENAESREYAMNNIENYSLKNSADILS
                     EITNSTVSYTSPSPEVYSDVLTKAGVPGEYVGMFVGFGAAIEQGEFYADSSDLEKLLG
                     RKPISLEDFLKQTYTK"
     gene            1788..>2250
                     /locus_tag="PLQ70_11770"
     CDS             1788..>2250
                     /locus_tag="PLQ70_11770"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HQE35338.1"
                     /translation="MNTLNDLHQISSSFSNTEKMPVLFLGHGSPMNAIEENQFVTGFR
                     NLAKTLPQPNAILCISAHWFTNGTKVTAMEMPRTIHDFGGFPQALFEVQYPTKGSPEL
                     AVETKELLQPIDVELDEHWGLDHGAWSVIKHLYPEANVPVIQLSIDYTKSPQ"
BASE COUNT          742 a          381 c          448 g          679 t
ORIGIN      
        1 cgtccttgac ttacgaagaa tcgaatagat tgtaccaaga agtcctgaaa gattacgaag
       61 gcgaaaccaa gtacaaacaa ttccaaaaag tgaaagccaa tgaatatttc aatggcttgc
      121 aagtcaagtt ttcgtatgcc ataacttgcc acaaatcgca aggagggcag tggaatacgg
      181 tttttgtcga acagccgtat ttgccggatg ggattaacag ggattacatt cgctggcttt
      241 atacggctat gacgagagca aaaaataagc tctatttgat aggatttaag gacgagagtt
      301 ttgtggatta actttctgaa agcaccgttt cacgatgttg caaaccccat tctaacattt
      361 tatccaaaac atcgcccaat gattttgcag attcagtcaa ttcatattct accgtgatgg
      421 gtgttgtgtc ataaacggtt cttttgacca ttccgttcat ttctaaatct ttcaattctt
      481 tggacaacat tcgaggtgtg atttttggaa tattgcgttg aatttcggta aaacgtcttt
      541 tttcgaacaa taaagaacca ataatgggta atttccattt gccattaatt acgtttaaag
      601 tgtcgtttac agccaataca aattgaatag gacaggcttt ggcttctttg aaactgattt
      661 tattttccat tatttccttg attttgttgt tactatacaa aaggatacta ctatacttta
      721 atgcatcgct atattttagg tagcaaatgt agttatcttt gcattagtat taacaataaa
      781 aattaaaaaa tgagtgcaaa aattttagta actggcgcaa caggtaattt tggaaaaaca
      841 accatcgatt ttttattaaa aaaaggaatc gcagcaacca atatttctgc tttggtaaga
      901 gatgaggcca aagccgaaga tttaaaagca aaaggcatca caattgttaa aggagattat
      961 gataattatg attctttgct agcagcattt aaaggagtgg ataagttatt gttggtttcg
     1021 gctactgatt tagaaaatcg tagcaaacaa caattgaatg cagtaaaagc ggccaaagaa
     1081 gccggtgtaa aacacatttt atacaccagt tttgaacgaa aaaatgagtc ggaaacctcg
     1141 ccgattgctt ttttggcaaa atcgcatatt gataccgaca acgccattaa agcgagcgga
     1201 atgacgtata gtatttttag aaataactta tatttagagg tgttaccaat gttttttggc
     1261 gaacaagttt tagcaacagg aatctttttt ccagctggag ataccgctgc agcttatgtt
     1321 tctagaaatg atttggcaga agctgttgcc aatgtcttaa ttgctgaaaa tgctgaaagc
     1381 cgagaatatg caatgaataa catcgagaat tattcattga aaaacagtgc ggatattttg
     1441 agtgaaataa ccaatagcac agtaagttac acaagtccaa gtcctgaagt ttattcagat
     1501 gttttaacaa aagcgggcgt tcctggagaa tatgttggaa tgtttgtagg ttttggagca
     1561 gcgattgaac aaggtgaatt ttatgccgat tcatcggatt tagaaaaatt attgggtaga
     1621 aaaccgattt ccttggaaga tttcttgaag caaacgtaca caaaataaga actatagaat
     1681 caagattaga aaaacgggca attttattga tccgtttttt catttaatga ctacctgaac
     1741 ggaaaagatt ttttacattt aaacaatatt tatcgcgatt agattatatg aatactttaa
     1801 acgatttaca tcaaatttca agcagttttt ctaacaccga aaaaatgcct gttttgttcc
     1861 tgggacacgg aagtccgatg aacgccatcg aagaaaatca gtttgtgact ggctttcgaa
     1921 acctagccaa aactttgcca caacccaacg ccattttgtg tatttcggcg cattggttta
     1981 ccaatggaac caaagtcaca gcaatggaaa tgcccagaac aattcacgat tttggaggtt
     2041 tcccgcaagc cttattcgag gtgcaatatc ctacgaaagg aagtccagaa ttggcagtgg
     2101 aaaccaagga attgttgcaa ccaattgacg tggaattgga cgaacactgg ggtttggatc
     2161 acggagcttg gagtgtcatt aaacatttgt atcccgaagc caatgttccc gtaatccagt
     2221 tgagtattga ttacaccaaa tcgccgcaat
//
LOCUS       DAOSNN010000314         2207 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_49390_length_2207_cov_7.274628, whole genome shotgun sequence.
ACCESSION   DAOSNN010000314 DAOSNN010000000
VERSION     DAOSNN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49390_length_2207_cov_7.274628"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLQ70_11775"
     CDS             complement(<1..1064)
                     /locus_tag="PLQ70_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQE35339.1"
                     /translation="MQYFNFIGAYKAKGKYKKTYRDAKQSYLNRIRWGVSMFYFGMGL
                     CFSSWASRIPTIKAALNLSDGELGTILFALPIGQLTMMFFSGKLVTRFGSERTLPFAI
                     LMYAFSLTNIGLAQNAWQLALGLVSFGIFGNLTNISVNTQGVYTEGIFRRTIMSSFHG
                     MWSLAGFTGALVGLGMLALKITTYMHFVIVAFVVVVLVAINFKYLIKAKEKVRPAKKK
                     IFSKPDKSLILLGVIGFCSMASEGIMFDWSGVYFKDIVKAPGSLIVVGYTSFMIMMAG
                     GRFFGGYLIDKFGRKTVMQISGVMISSGLYIAVFLPYIIPCTLAFMIVGLGVSTVIPT
                     LYSIAGKHPTIPTGEALTAVS"
     gene            complement(1161..2204)
                     /locus_tag="PLQ70_11780"
     CDS             complement(1161..2204)
                     /locus_tag="PLQ70_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQE35340.1"
                     /translation="MQKIKTALLSYGMSGKVFHAPFLELHPGFELLGSWERSKKLIQE
                     DYPEVKSFPSLESILEDKSIDLVVVNTPIDTHFEYAKKVLLAGKHAVVEKAFTANVAE
                     AIELEALAEEKGLKLAVFQNRRWDSELKTVKKVLDEKLLGEIVEAEFHFDRYNPILSP
                     KIHKETVNSGSGILKDLGPHIIDQALYLFGLPNAVFADIRITREHSLVDDYLDILLYY
                     SDVRVRLKAGFFVREPNPSYVVHGKKGSFLKSRGDVQEDELKLGKKPNLTTWGTEKEG
                     QEGLLHTEIDGKIIKEKVPTLQGNYYDFFDGVYQAIANDKLEPVTAQDGVNVMRIIEA
                     ATQSNSQKKVIDL"
BASE COUNT          706 a          473 c          355 g          673 t
ORIGIN      
        1 gaaacagctg tcaaagcttc gccagttggg atggttggat gttttcccgc aatactataa
       61 agtgttggaa taacggtcga aactcccaaa ccaacaatca taaaagcaag agtacacgga
      121 ataatatacg gaagaaatac cgcaatgtat aagcctgagg aaatcattac accgctaatt
      181 tgcatcacag tttttcgtcc aaatttatcg attaaataac ctccaaaaaa ccgtccaccc
      241 gccatcatta tcataaaaga ggtataaccc acaacaatta atgaacctgg agctttgacg
      301 atatctttga aataaacgcc actccagtca aacataattc cttcacttgc catactacaa
      361 aaaccaatta cacccaacaa aatcaatgac ttatcgggtt tgctaaatat ttttttcttg
      421 gcagggcgaa ctttttcttt ggcttttatc aaatatttga aattgatggc taccaatact
      481 actactacaa aagctacaat aacaaaatgc atataagttg taatttttag tgccaacatt
      541 cccaatccga ctaaggctcc tgtaaatcct gctaaactcc acattccatg aaaagaggac
      601 ataatagttc ttctgaaaat tccttccgta taaacgcctt gtgtatttac tgaaatattg
      661 gtcaaattgc caaagattcc aaaagaaacc aatccgagtg ccaattgcca agcattttgt
      721 gccaaaccga tattggttaa actaaaagca tacatcaaaa tggcgaaagg aagtgtacgt
      781 tcactaccaa aacgggtaac aagtttaccc gaaaaaaaca tcattgtcaa ttgtccaatt
      841 ggtaaggcaa atagaatcgt acccaattcg ccatcgctta aatttaaagc agctttgatg
      901 gttggaattc gacttgccca actcgaaaaa cacaaaccca ttccaaaata gaacatagaa
      961 acaccccaac gaattcgatt caaatacgat tgtttggcat cacgataggt ctttttgtat
     1021 ttgccttttg ctttgtaagc tccaataaaa ttaaaatact gcaaatcgtt tcttttttag
     1081 aattgaatct gcaaaaatac aaaatcaatc tgtttctaat gggcgtttcc tttataaacc
     1141 gaacaataca acgttattgt ttataagtcg attactttct tttgagagtt actctgagta
     1201 gctgcttcaa taattcgcat cacattcacg ccgtcttggg cggttactgg ttccaatttg
     1261 tcattggcaa tcgcttgata tacaccatca aaaaagtcgt aataattacc ctgaagtgtt
     1321 ggtacttttt ctttgataat tttaccatct atttcggtgt gcaaaagccc ttcttgacct
     1381 tctttttcgg ttccccaagt ggttaaatta ggttttttac ctagtttcaa ctcgtcttcc
     1441 tgaacatcgc cacgcgattt caaaaatgaa cctttcttgc cgtgaacaac ataagaaggg
     1501 ttgggttctc tcacaaaaaa tccagctttc aatcgcactc ttacatctga ataatacagt
     1561 aaaatatcta aataatcatc gactaacgaa tgttctctcg tgattctaat atcggcaaaa
     1621 acagcattgg gcaaaccaaa taaatacaag gcttggtcaa taatgtgagg gcctaaatct
     1681 ttcaaaattc cagaaccaga attgacggtt tccttgtgta tttttggact caaaatagga
     1741 ttgtagcggt caaagtgaaa ttcggcttct acgatttcgc ccaacaactt ctcgtcaaga
     1801 acttttttca cggttttgag ttcgctgtcc catcttcgat tttgaaaaac cgccaatttt
     1861 aaaccttttt cttcggccaa agcttccaat tcaattgctt cggcaacgtt tgctgtaaat
     1921 gctttttcga caacagcgtg ttttccggca agcaacactt ttttggcata ctcaaaatgg
     1981 gtgtcaattg gcgtgttcac cacaaccaaa tcgatggatt tgtcctccaa aatcgactcc
     2041 agactaggaa aacttttgac ttcgggataa tcttcttgaa ttaatttttt gcttctttcc
     2101 caagaaccca gaagttcaaa accggggtga agttccaaaa acggggcgtg aaaaactttt
     2161 cccgacattc cgtaagataa gagtgctgtt tttatttttt gcatttt
//
LOCUS       DAOSNN010000315         2200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_49624_length_2200_cov_4.318415, whole genome shotgun sequence.
ACCESSION   DAOSNN010000315 DAOSNN010000000
VERSION     DAOSNN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49624_length_2200_cov_4.318415"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLQ70_11785"
     CDS             complement(<1..646)
                     /locus_tag="PLQ70_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannanase"
                     /protein_id="HQE35341.1"
                     /translation="MKNRLLKTLALALVFSTIACQAQEKITVKGTQFFKGDQPYSYIG
                     TNYWYGSLLASKKVGDRKRLLRELDLMQKNGIDNLRILVGGDGGKYDFTVRPALQYAQ
                     GKYDQDLLDGLDFLIAEMGKRKMYAVLFLTNNWEWSGGMSQYLEWSGKGAIPIPAIPP
                     NTWPQYMAYTEQFYSCESCMEALNNHVKFIMGRTNVYTKRKYTEDNTIMAWEVGN"
     gene            complement(776..2008)
                     /locus_tag="PLQ70_11790"
     CDS             complement(776..2008)
                     /locus_tag="PLQ70_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HQE35342.1"
                     /translation="MKKTYLKIIISSFTIAFSSCSKDDPTPAPVVVVPPAVVTDVLTT
                     TNAKTYMVDPNATAETVALFYNLKKLAKTKFAIGQQDAFNSFYNNGSSAQSDIKKTTG
                     YDPALLGSDFMFITDKNNNGQANNWFYQQEVIITDDVKEAYAKGMINIFCWHLREPNK
                     EDSFYASDLTDAAKTTAFKSILPGGINNAWYKTKLDKVESVVSNLKGSNGELIPIIFR
                     PFHEFDGSWFWWGANFCSPEDYKAAYQFTVDYLKNTKGVHNILYAFSPDNSYSTSTNY
                     LSRYPGDAYVDVLGMDNYGDFNNQGQTGSDRANAKLKMISDLAVTKVKIAALTETGYQ
                     VTTTTPVINGWFSNYLYNALTANNVEISFVMFWNNTTSGYWVPTPTDSNAADFKTFAT
                     KPESVLVNTLPKMYILPK"
BASE COUNT          625 a          427 c          384 g          764 t
ORIGIN      
        1 cgtttccaac ttcccaagcc ataatggtgt tgtcctccgt gtattttctt ttggtataaa
       61 cattagttcg tcccataata aatttcacat gattgtttaa tgcttccata caagattcgc
      121 aactgtagaa ttgttcagtg taagccatat attgcggcca agtattaggc ggaatcgcag
      181 gaattggaat tgctcctttt ccagaccatt ctagatattg cgacattccg cctgaccatt
      241 cccagttgtt ggtcaaaaat aaaacggcgt acatcttgcg tttgcccatt tctgcaatca
      301 agaaatccag accgtcaagt aaatcctggt cgtattttcc ttgtgcatat tgcaaagcgg
      361 gacgaacggt aaaatcgtat tttccgccat cgccacccac taaaatacgc aagttgtcga
      421 tgccgttttt ttgcatcaaa tctagctctc gcaacaatct ttttcggtcg cccactttct
      481 tagaagctaa taaacttcca taccaataat tggttccaat gtaagaatag ggttgatctc
      541 ctttaaagaa ttgagttcct ttgacggtga ttttttcctg tgcttgacac gctattgttg
      601 aaaaaaccaa ggctagagca agtgttttta aaagtctatt tttcatatct atttttttag
      661 aagctgtttc ctgctattcg ttacaaacga agttcactga actccactgc tatcagggct
      721 agggcatttc gtttataatt atatctctac aaatttaaaa ttctatgctt tatttttatt
      781 taggtaatat atacattttt ggtaaggtat ttaccaaaac tgattccggt ttggttgcaa
      841 atgttttaaa atcggcagcg ttggaatcag ttggagtagg tacccaatat cccgaagtgg
      901 tattattcca aaacatcacg aaactaattt caacgttgtt ggccgttagc gcattgtata
      961 aatagttcga aaaccatcca tttattactg gagttgttgt cgttacttga taacctgttt
     1021 cggtcagggc ggcaattttt actttagtta cagccaaatc tgaaatcatt ttcagtttgg
     1081 cattggctct atccgaaccc gtttgccctt gattgttgaa atcaccataa ttatccattc
     1141 ccaaaacatc aacataggca tcgccaggat aacgactcag gtaattggtt gatgtggaat
     1201 aggaattatc gggagaaaaa gcatataaaa tgttgtgaac tcccttggtg ttttttagat
     1261 aatctaccgt aaattgatag gctgctttat agtcttcggg agaacaaaaa ttagctcccc
     1321 accaaaacca actgccgtca aattcgtgaa aaggcctgaa aataattgga atcaattcgc
     1381 cattacttcc ttttagattc gaaactaccg actccacttt gtcaagcttt gttttgtacc
     1441 aagcgttgtt gattccgcca ggcaaaatgc ttttgaaagc ggttgttttt gctgcatcgg
     1501 tcaaatccga agcataaaaa ctatcttcct tattgggttc tctcaaatgc caacaaaata
     1561 tatttatcat tcctttcgca taagcttctt tgacatcatc ggttatgata acttcttgct
     1621 ggtaaaacca gttattggct tgaccattgt tgtttttatc ggtaataaac ataaaatcgg
     1681 aacctagaag agcaggatca tatccagtag ttttcttaat gtctgactgt gctgaggaac
     1741 cattattgta aaaactattg aaagcatctt gctgaccaat ggcaaatttt gttttcgcta
     1801 atttcttcaa gttgtaaaac aaagcgactg tttctgctgt agcattcgga tccaccatat
     1861 aggtttttgc atttgttgta gtcaaaacat cggtaaccac tgccggtgga actaccacaa
     1921 cgggagctgg cgttggatcg tctttggagc aactactaaa ggctattgtg aaactagaaa
     1981 taattatctt taaataggtt tttttcatta ttttgttttt aagaaccgct ttttgttcta
     2041 aaattatatt gacttttttt agaattttac ttctaattta ctaccattat atttcacttt
     2101 ttggccttca tttgcgtttg aagtcaaatt aaatgtcgca tttttatttt tgccaataaa
     2161 gacaatattg aaaattctat tcttttgcat tccatcaaaa
//
LOCUS       DAOSNN010000316         2188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_50005_length_2188_cov_4.745429, whole genome shotgun sequence.
ACCESSION   DAOSNN010000316 DAOSNN010000000
VERSION     DAOSNN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50005_length_2188_cov_4.745429"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLQ70_11795"
     CDS             <1..462
                     /locus_tag="PLQ70_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQE35343.1"
                     /translation="YQRSLYNHIRNIVLDHDDANDVLQNTFVKVFQNLNKFKGESKLF
                     SWMYRIATNEALTFLNQKAKKNGVSSETLQNKTIDNLKADVYFDGNDIQIKLQKAIVL
                     LPEKQQLIFKMKYFEELKYEEISTILGTSVGALKASYHLAVKKIEAFITKN"
     gene            493..906
                     /locus_tag="PLQ70_11800"
     CDS             493..906
                     /locus_tag="PLQ70_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35344.1"
                     /translation="MKTFKLENEPKIATGFTVPENYFEDFSTRMMQQLPEKETKVISI
                     FARRKTWIYAAAAVLVLALSIPIYNNFYSHSSEIDDATLENYITYHSSVSDTDLVNLL
                     DEQDIQKMNIDLNLEDKTIENELSENSNLEQYLLN"
     gene            913..1365
                     /locus_tag="PLQ70_11805"
     CDS             913..1365
                     /locus_tag="PLQ70_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor of ECF-type sigma factor"
                     /protein_id="HQE35345.1"
                     /translation="MKTSKFLLISILFISIHSFAQSQMEEKREQIKTLKVGFITNELA
                     LTPDEAAKFWPIYNAYEDKQFEIKHQKMKAFKERMDSDLDKMNEKEASALLAQMQSND
                     DELYQIRKKFISNLKNILPATKIIKLKKAEDDFNRKLLQQYRNKGSRK"
     gene            1596..>2188
                     /locus_tag="PLQ70_11810"
     CDS             1596..>2188
                     /locus_tag="PLQ70_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35346.1"
                     /translation="MKTQVLLFALMTVVFATQTQGQNRTIVNAQNSEISDNLDLRVVA
                     SVFGDSKNLEDFERRLNDPKLQISNLDLNYDGQVDYLRVIESIEKRTHIIIIQSVLNR
                     DLYQDVATIEVEKNWNNNVRIQVVGNSYFYGDNYIYEPVYYYTPIIYANFWMSNYRPY
                     FSNWCWNYYPSYYYAWNSYPVFRYRYNVNVCLNNYSY"
BASE COUNT          867 a          308 c          314 g          699 t
ORIGIN      
        1 tatcaaaggt cgctatacaa tcatattaga aatattgttt tggatcacga tgatgccaat
       61 gatgttttgc aaaatacctt tgtaaaagtg tttcaaaatt taaacaaatt caaaggagaa
      121 agtaaattat tttcgtggat gtatcgaatt gcgactaatg aagcattgac ttttttaaat
      181 caaaaagcaa aaaagaatgg agtttcatct gaaactttgc aaaacaaaac catcgataat
      241 ctaaaagcag acgtttattt tgatggaaac gatattcaaa taaaattgca aaaagcaatt
      301 gtattattgc ccgagaaaca acaattaatc tttaaaatga aatattttga agaactaaaa
      361 tacgaagaaa tctctacaat acttggcaca tcggtgggcg ctttaaaagc atcttatcat
      421 ctcgctgtca aaaaaataga agcctttata acaaaaaatt aaaccttttg acttcaatta
      481 agtctaaacg atatgaaaac atttaaacta gaaaacgagc ctaaaatagc aactggattt
      541 accgttccgg agaattactt tgaggatttt tcgacaagaa tgatgcaaca attacccgaa
      601 aaagaaacca aagtaatttc tatttttgca aggagaaaaa cttggattta tgctgctgca
      661 gctgttttgg tacttgcgtt atcaattccg atttataata atttctatag tcattcgtca
      721 gaaattgacg atgcaacttt ggaaaattat atcacttatc attcctctgt ttcagacacc
      781 gatttggtaa atcttttaga tgaacaagac attcaaaaaa tgaatatcga tttgaattta
      841 gaagataaga ctatcgaaaa tgaactttct gaaaacagca atttagaaca atacctatta
      901 aattaataca aaatgaaaac aagcaaattt ttactaatta gtatattatt tatttccatt
      961 cattcctttg cgcagtctca aatggaagaa aaaagggaac aaataaaaac tttgaaagtg
     1021 ggatttataa caaatgaatt agcgttaacc cctgatgaag cagctaaatt ttggcccatt
     1081 tataatgctt atgaagacaa acaatttgaa ataaaacatc aaaaaatgaa agccttcaaa
     1141 gaacgtatgg attctgattt agacaaaatg aacgaaaaag aagcatcggc tcttcttgct
     1201 caaatgcaaa gtaatgacga tgaactatat caaattagaa aaaaattcat ttcgaaccta
     1261 aaaaatattt tacctgcaac aaaaattata aaattaaaaa aagccgaaga cgattttaat
     1321 agaaagttat tgcaacaata tcgaaataaa ggttctagaa aataaaatca ttttttcttt
     1381 agaattccat caaaaaccaa gaaatcattg aaacgatttc ttggtttttt tgtggaaaca
     1441 aatcaaaaag aaaaaacatt ttaaacattt ttacaaattg caattaatta attttaagtt
     1501 gtttaaaaat atttttacac aatcgaccaa tgaacatatg aataattggc acagtaattg
     1561 gtttattcaa aacaaataac ttaatcttat ttattatgaa aactcaagta ttactattcg
     1621 ctctaatgac tgtggttttt gcaacccaaa cacaaggaca aaacagaaca attgtaaatg
     1681 cacaaaattc tgaaattagt gacaatttag atttaagagt agttgcatcg gtttttggtg
     1741 attcaaaaaa cttagaagat tttgaaagac gattgaatga tcctaaatta caaatttcaa
     1801 atttagattt gaattatgat ggtcaagtag attatttaag agtaattgaa tctatagaga
     1861 aacgcaccca catcatcatc attcaatcgg ttttgaatcg tgatttatat caagatgttg
     1921 ccacaatcga agtagaaaaa aactggaaca ataacgttcg tattcaagta gtaggtaatt
     1981 cctatttcta tggcgataat tatatttatg agcctgtata ttattatact ccaattattt
     2041 atgccaactt ttggatgtca aattatcgtc cttatttttc taattggtgt tggaattatt
     2101 atccaagtta ttattatgct tggaattcct atccagtatt tagataccga tataatgtaa
     2161 atgtttgtct gaacaattat agttataa
//
LOCUS       DAOSNN010000317         2175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_50421_length_2175_cov_5.564151, whole genome shotgun sequence.
ACCESSION   DAOSNN010000317 DAOSNN010000000
VERSION     DAOSNN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50421_length_2175_cov_5.564151"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1012)
                     /locus_tag="PLQ70_11815"
     CDS             complement(77..1012)
                     /locus_tag="PLQ70_11815"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HQE35347.1"
                     /translation="MKSILIFLLMPFMMLAQIKKDSIKVFYLGGQSNMEGFGYVKNLP
                     DSLKKKNKDFFIYHGNSVVDNDVTGGLGKWDVLQPGHGTGFSSDGKTNQLSDRFGVEL
                     SLAKKLQELYPNQKLAFIKYARNGSSIDSTGTTSFGAWEPDFKGGKGMNQYDYFLKTV
                     HNAMAVKDINGDGVEDILVPSGIIWMQGESDSDKTEAIANQYYFNLKRLMELMRATFR
                     NNDLPIVIGKISDSGDIEGGKVWKFGELVQYNQEKFAATQPNVAIVRTTNSYKYSDRY
                     HYDNNGFIDLGKQFATKIFLLNTKNTKNSKKASLN"
     gene            complement(1055..>2175)
                     /locus_tag="PLQ70_11820"
     CDS             complement(1055..>2175)
                     /locus_tag="PLQ70_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HQE35348.1"
                     /translation="KEKGIGYSLLRTTIHSSDFASGSYTYIKEGDKDLKTFNIDYDRQ
                     YRIPMIKEAIKTAGGKITTYASPWSPPAFMKNTNNMLKGGTLLPEYYQPWANYFTKFI
                     KAYEKEGIPIWGITVQNEPMATQTWESCIFSAEAERDFLKNFLGPTMKKEGLGDKKIV
                     VWDHNRDLMTHRANIIFSDPEASKYAWGMGFHWYENWSGGSSMFDNVRKVYENFPTKN
                     LLFTEGCVEKFDAKKYQFWANGERYGISMINDFNNGTVGWTDWNILLDQNGGPNHVGN
                     FCFAPVHADTNTGELIYTPSYYYIGHFSKFIREGAKRVSNSVSRSNLLSTSFLNVDGK
                     MTTVVLNQSNKSVTYNLIVGSEKSVVTIPAHAIQTLVY"
BASE COUNT          636 a          425 c          343 g          771 t
ORIGIN      
        1 taatttattt aaatttgtct aataatctgt atcgattatt taggactagt caattttatt
       61 tcttttttac aaattcctaa ttcaaagatg cttttttaga atttttggtg tttttagtat
      121 tcagcaggaa aatttttgtt gcaaattgtt ttcctaaatc aataaatccg ttgttgtcat
      181 aatggtatct gtctgaatat ttataactat ttgtagttct tacgatggct acattgggtt
      241 gggttgcagc aaatttttcc tgattatatt gtaccaattc accaaatttc caaacttttc
      301 ctccttcaat atcgccagaa tctgagattt ttccaattac gataggcaaa tcattatttc
      361 taaaagtagc tcgcattagt tccatcaacc ttttcaaatt aaaataatat tgatttgcga
      421 tggcttctgt tttgtcagaa tcactttcgc cttgcatcca gatgatgcca cttggaacca
      481 aaatatcttc gacaccgtca ccattgatgt cttttacagc cattgcatta tggactgttt
      541 taagaaaata atcgtattga ttcattcctt ttcctccttt gaaatcgggt tcccaagcac
      601 caaaactagt tgtaccagtg ctgtcaatgg aagagccatt tctagcatat tttataaaag
      661 ctagtttttg gtttggatat aattcttgca attttttggc taacgaaagc tctactccaa
      721 atcggtctga aagttggttt gtttttccat ctgacgaaaa acctgttccg tgacctggtt
      781 gaagcacatc ccactttcct aatcctccag taacatcatt gtcaacaacg gaatttccgt
      841 gataaatgaa aaaatcttta ttctttttct ttaaagaatc aggcaaattt tttacataac
      901 caaaaccttc catattagat tgaccaccca aataaaacac tttgatgctg tcttttttta
      961 tttgtgccag catcataaag ggcattaaaa ggaatattaa aattgatttc ataatcataa
     1021 ataattaaaa taaataaaac actcagagct gtttctaata aactaaggtc tgaattgcgt
     1081 gtgctggaat ggtaacaact gatttttctg aacctacaat caaattataa gtaacacttt
     1141 tgttgctttg attcagcact acagttgtca tttttccatc gacattaaga aacgaagtac
     1201 tcaataaatt gcttctactt actgaattgc tcactctttt ggcaccctca cgaatgaatt
     1261 tcgaaaaatg acctatgtaa taataactcg gtgtgtaaat taattcgccc gtgttagtgt
     1321 ctgcatgaac aggggcgaag caaaaattcc ccacatgatt tgggcctccg ttttggtcta
     1381 aaagaatatt ccaatctgtc caacccaccg ttccattatt gaaatcgtta atcatcgaaa
     1441 ttccgtaacg ttcgccgtta gcccaaaatt ggtatttttt ggcatcaaat ttttcgacac
     1501 atccttcggt gaacaataaa tttttggttg ggaaattctc gtaaactttt cgaacattat
     1561 caaacattga cgaaccgcct gaccaatttt cgtaccaatg aaaacccatt ccccaagcat
     1621 attttgaagc ttctgggtcg gaaaaaataa tattggctct gtgtgtcatc aaatcacgat
     1681 tgtgatccca aaccacaatt tttttatccc ccaaaccttc ttttttcata gttggcccca
     1741 agaaattttt caagaaatct ctttcggctt cagcgctaaa aatacaagat tcccaagttt
     1801 gagtagccat tggttcgttt tgaacggtaa ttccccaaat agggattcct tctttttcat
     1861 aagccttgat gaatttggta aaataattgg cccaaggctg gtaatattcc ggaagcaatg
     1921 tgccaccttt tagcatattg ttagtgtttt tcataaaggc aggtggactc caaggcgatg
     1981 cgtaagttgt tatttttccg cctgcagttt ttatggcttc tttgatcatt ggaatgcgat
     2041 attgcctgtc gtaatcgata ttgaacgtct ttaaatcctt gtcgccttct ttgatatacg
     2101 tatagcttcc gctggcaaaa tcagaactat gaatcgtggt tcttagcaag gaataaccaa
     2161 ttcctttctc tttgt
//
LOCUS       DAOSNN010000318         2147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_51346_length_2147_cov_4.676386, whole genome shotgun sequence.
ACCESSION   DAOSNN010000318 DAOSNN010000000
VERSION     DAOSNN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51346_length_2147_cov_4.676386"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..679)
                     /locus_tag="PLQ70_11825"
     CDS             complement(35..679)
                     /locus_tag="PLQ70_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HQE35349.1"
                     /translation="MSINSIFQFLVPKDKKFFPLFEESSSNLVQLASILHEAVNLPLK
                     ERDELFLKIDELEQKGEDITRLTNLELSRNFITPFDREDIHSLITSIDRVADNLHGAS
                     SRMRLYQVAKITKSIRKLTEINLEACQNIDSAVKELRDLKKMKKITDACSRISKLEAK
                     SDSVYDKAVADLFENETDAKNIIKYKEVLSVLETATDKCKGVASVLESISVKHS"
     gene            complement(758..>2147)
                     /gene="hutH"
                     /locus_tag="PLQ70_11830"
     CDS             complement(758..>2147)
                     /gene="hutH"
                     /locus_tag="PLQ70_11830"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HQE35350.1"
                     /translation="NIQKCRDYLDAKMGSNSKPIYGINTGFGSLCNVKISNKNLSKLQ
                     ENLVKSHACGTGEEVPVAIVKLMLLLKIQSLSYGHSGIQLQTVERLVDFYNNDILPVI
                     YTQGSLGASGDLAPLAHLSLPLLGEGEVFFDGKKVHSGVVLQHFGWEPIILQSKEGLA
                     LLNGTQFMSAYGVHILMKACHFSYLADLIGTISLEGFDGRLEPFHELIHSIRPHKGQI
                     VTAKRVTQFLEGSEIIKQEKKHVQDPYSFRCIPQVHGASKDAIDYAKNVIKTEVNSVT
                     DNPNIFIETDEIISGGNFHGQPLALALDFLAIALAELGSISERRTFQLISGLRNLPAF
                     LVDNPGLNSGLMIPQYAAASIASQNKQLATPASVDSIVSSNGQEDHVSMGANAATKAL
                     RVMDNLERILAIELLNASQAIEFRRPLKSSDFIEQFLKSYREEVSFVKEDRILHYDID
                     KTIVFLNRFQQE"
BASE COUNT          696 a          417 c          331 g          703 t
ORIGIN      
        1 cagatgcgga gattgattat gatgattgcg gtggttacga atgttttaca gatatagatt
       61 ctaaaacact agcgacacct ttacatttat cagttgcagt ttctaaaacc gataatactt
      121 ctttgtattt gataatgttt ttagcatcgg tttcgttttc aaaaagatcg gcaaccgctt
      181 tgtcataaac gctgtctgat ttagcttcca atttgctaat tctagaacaa gcgtccgtga
      241 tttttttcat ttttttcaaa tctctcaatt ccttcacagc actatcgata ttctgacaag
      301 cctcaagatt aatttcagtc aattttcgaa tagattttgt aatctttgct acttgataca
      361 agcgcattct actagaagca ccgtgaagat tatcggcaac tctgtctatt gaagtaatca
      421 aggagtgaat atcttctcta tcaaacggag tgatgaaatt tctactcaat tcaagattgg
      481 tcaaacgggt aatgtcctcc cctttttgct ctaactcatc tatttttaaa aaaagttcgt
      541 ctctttcttt caatggaaga tttacggctt cgtgcaaaat tgaagctaat tgtaccagat
      601 ttgatgaaga ttcctcaaaa agaggaaaga attttttatc ctttggaacc aaaaactgaa
      661 aaatactatt tattgacatt ttaataatgt ttaattaatt tgaatgagca aaattacgtc
      721 tttattttat gctaatgtta agtaattgtt aacaatttta ttcttgctga aatctattaa
      781 ggaacactat ggttttgtca atatcataat gcaaaattcg atcttctttt acaaatgaaa
      841 cttcctcacg ataggatttt aaaaactgct caataaaatc acttgatttt aaaggtctcc
      901 tgaattcaat ggcttgcgat gcattcaata attcaatggc aagaattcgt tccaaattat
      961 ccataacacg caaggctttt gtagctgcat ttgcccccat actcacatga tcttcttgac
     1021 cattactcga cacaatacta tcaacacttg ccggtgtagc caattgtttg ttttgactag
     1081 caatactcgc agctgcgtat tgcggaatca ttaaacccga attcaaacca gggttatcga
     1141 ctaaaaaagc aggaagattt ctcaaacccg atatcaattg aaaagttctt cgctcagaaa
     1201 tgcttcccaa ttcagccaaa gcaatggcta aaaaatctaa agctaaagcc aaaggttgtc
     1261 cgtggaaatt tcctcctgat ataatctcgt cagtttctat aaatatatta ggattatcgg
     1321 taaccgaatt gacttcggtt ttgatcacat tttttgcata atcgatagca tcttttgaag
     1381 caccgtgaac ttgcggaata catcgaaaag aataaggatc ttgaacatgc tttttctctt
     1441 gtttgataat ttcgcttcct tccaaaaatt gagtaacccg tttggctgta acaatttgcc
     1501 ctttatgcgg tcgaatggaa tgaattaact catgaaaagg ttctaatctt ccatcaaatc
     1561 cttctaaaga aatagtgcca atcaaatcgg ctaaatatga gaaatgacag gctttcatca
     1621 aaatatgaac accataagca ctcataaatt gagttccatt caacaaagct aatccttctt
     1681 ttgattgtaa aattattggt tcccatccaa aatgttgcaa aacaacacct gaatgtactt
     1741 ttttaccatc aaaaaacact tcgccttcac ctaataaagg caaagacaaa tgcgccaaag
     1801 gtgctaaatc gcccgaagca ccaagcgatc cttgagtata aattactggc agaatatcat
     1861 tattgtagaa atcgaccaat cgttctaccg tttgtaactg aattcccgag tgaccataac
     1921 tcaatgattg aattttgagc aacaacatca atttgacaat ggccacagga acctcttcgc
     1981 cagttccaca agcatgagat tttaccaaat tctcttgtag tttggataga tttttattag
     2041 aaattttaac attacaaagc gaaccaaagc cagtattgat accataaata ggtttagaat
     2101 tcgaccccat tttggcatcc aaataatccc gacatttttg aatattt
//
LOCUS       DAOSNN010000319         2141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_51563_length_2141_cov_3.140940, whole genome shotgun sequence.
ACCESSION   DAOSNN010000319 DAOSNN010000000
VERSION     DAOSNN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51563_length_2141_cov_3.140940"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLQ70_11835"
     CDS             complement(<1..421)
                     /locus_tag="PLQ70_11835"
                     /inference="COORDINATES: protein motif:HMM:NF039702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6250 domain-containing protein"
                     /protein_id="HQE35351.1"
                     /translation="MKSIYLSFVFSVFTIALFSQSRMKDVEEMKVIYSDNFDKDWSKW
                     DVEFEKPETSSLKIIDGKLDVSTSIGATIWFETQLSGNIMITYDVIVIDKGGAFDRVS
                     DLNAFWMATDLSSKTIQRDGKFSSYDNLNLYYAGVGGH"
     gene            527..1054
                     /locus_tag="PLQ70_11840"
     CDS             527..1054
                     /locus_tag="PLQ70_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35352.1"
                     /translation="MNDYLKKIKLAQEINIELQISKSDFTKKFRENVDESDLGFEPFE
                     VFKTSKNEYKGNIENNRFELKKRRKLFDTNYSFAKATGIFVQETDKLIIKTLITGFQK
                     KMLFFVGFLVFFYAIFLSSIFLADDNAPKFFILPFLLFHAALMLGIPYFMMRRSVKRM
                     IYDLERDFHYWVTKN"
     gene            complement(1063..>2141)
                     /locus_tag="PLQ70_11845"
     CDS             complement(1063..>2141)
                     /locus_tag="PLQ70_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007138124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQE35353.1"
                     /translation="VYEPIAANLLCNRMEEMIAQTGQNGFHYVDEAAPPALMRALALE
                     ILRRKLSVTWWTNIRFEKSFTRDLCLLLKASGCIAVSGGLEVASDRLLKLIDKGVTVE
                     QVAKVTRNFTEAGIMVHAYLMYGYPTQTVQETVDSLEMVRQLFELGILQSGFWHQFAM
                     TAHSPVGMYPEKFGVVKKTEAIGTFANNDLNYLDKTGVDHEKFSFGLKKSLFNFMHGI
                     CFDYELQEWFDFKIPKTKIHPDFIANALEEDEDFNIKPTAKIVWLGGKPLVEYFTKTK
                     KGNSWEMMTLTFHDKKESFTIQTGKNEGEWLVQILDKISVFQSKTYSFKEIKSDFERH
                     LEDFELFWYSKPVNTLREFGLLVL"
BASE COUNT          718 a          414 c          323 g          686 t
ORIGIN      
        1 aatgcccacc aacaccagca taatacaaat tcaaattgtc ataagaagaa aatttcccat
       61 ctcgttgtat tgttttagaa ctcagatctg ttgccatcca aaaggcattc aaatcagaaa
      121 ctcggtcaaa agcacctcct ttatcaataa ctattacatc ataagtaatc attatatttc
      181 cagataattg ggtttcaaac caaatggttg cgccaataga agttgaaaca tccaactttc
      241 catcaattat ttttagactc gacgtttctg gtttttcgaa ttcgacatcc catttcgacc
      301 aatctttatc gaaattatcc gaataaatga ctttcatttc ttctacatct ttcattcttg
      361 attgtgaaaa aagagcaatt gtgaaaactg aaaaaacaaa agataaataa atacttttca
      421 taaacgatat tttataatac caacaaacca aactccctta aagtgttctc gggttttgag
      481 taccaaaaca aaagtagtat atttgttaca catcaaacga aaaaccatga acgactattt
      541 aaaaaaaata aaactagcgc aagaaataaa cattgaattg caaatctcaa aatcagattt
      601 cacaaagaag tttagagaaa acgtggatga atccgattta ggttttgaac cgtttgaagt
      661 tttcaaaaca agtaaaaatg aatacaaagg aaatattgaa aataacagat ttgaattaaa
      721 aaaaagacga aaattatttg atactaatta ttcttttgca aaagcaacag gaatatttgt
      781 tcaagaaact gacaagttaa ttataaaaac attgatcact ggatttcaaa aaaagatgct
      841 tttctttgtt ggctttttgg tctttttcta cgcaatattc ctaagcagta ttttccttgc
      901 tgacgacaac gcacccaaat ttttcattct gccgttttta ttgtttcacg ctgcattaat
      961 gttgggaatt ccttatttta tgatgagacg aagcgtgaaa agaatgatct atgatttaga
     1021 aagagatttt cattattggg ttaccaaaaa ttaagattca atttataaaa ccaacaatcc
     1081 aaattccctc aaagtattga ctggtttgga ataccaaaac aattcaaaat cctccaaatg
     1141 cctttcaaaa tcggatttta tttctttaaa tgaatacgtt ttggattgga aaaccgaaat
     1201 tttatcaagg atttgcacca accattcccc ctcgtttttt ccggtttgaa tggtgaaact
     1261 ttcttttttg tcgtgaaaag tcaaggtcat catttcccag gaatttcctt ttttggtttt
     1321 cgtgaaatat tccacaagtg gtttccctcc tagccaaact atttttgcag tcggttttat
     1381 attgaaatct tcgtcttctt ccagagcatt agcaataaaa tcaggatgga tttttgtttt
     1441 cggaatttta aaatcgaacc attcttgcaa ttcatagtca aagcaaatgc cgtgcataaa
     1501 attgaagagc gatttcttta gtccaaaact gaatttttcg tgatcgacac ctgttttgtc
     1561 caagtaatta agatcgttgt tggcgaaagt tccaatggct tccgttttct ttaccacacc
     1621 aaatttctca ggatacatcc caacaggact atgagccgtc attgcaaatt gatgccaaaa
     1681 tcctgattgc aaaattccca attcaaataa ttgacgtacc atttccaaac tatccacggt
     1741 ttcctgaacg gtctgtgtgg gataaccata catcaaataa gcgtgaacca taattcctgc
     1801 ttcggtaaaa tttcgggtaa cttttgccac ttgttctacc gtcacacctt tgtcaatcaa
     1861 ttttaataat cggtccgagg ccacttcgag tcctccagaa acagcaatac atcctgaagc
     1921 tttcaatagc aaacataaat ctctggtaaa acttttttcg aaacgaatgt ttgtccacca
     1981 agttaccgac aatttcctgc gaagaatttc aagtgccaaa gcacgcatca aagcgggtgg
     2041 tgcggcttca tctacataat gaaacccatt ttggccggtt tgcgcaatca tttcctccat
     2101 tctattgcac aataaattgg cggcgatggg ttcgtaaact t
//
LOCUS       DAOSNN010000320         2117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_52318_length_2117_cov_4.106693, whole genome shotgun sequence.
ACCESSION   DAOSNN010000320 DAOSNN010000000
VERSION     DAOSNN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52318_length_2117_cov_4.106693"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLQ70_11850"
     CDS             complement(<1..234)
                     /locus_tag="PLQ70_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HQE35354.1"
                     /translation="MNLKKIITIATLVVITGLLIYGGILANQIFSKNTKFSQNEVFVF
                     VPTGATYDEVKIIIEPFVDNMDRFEMVANKRSYP"
     gene            complement(247..780)
                     /locus_tag="PLQ70_11855"
     CDS             complement(247..780)
                     /locus_tag="PLQ70_11855"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQE35355.1"
                     /translation="MITLKGENIYLRALEPNDLEFVYAMENDQSIWEVSNTQTPYSRF
                     LVKQYLENAHQDIYEAKQLRLAICQDEDFPALGLIDLFDFDPKNNRAGIGIVIQGEEN
                     RRQNIGSEALELLIRYGFYHLNLHQFYANISTENKASIALFTKFGFQKIGTKKDWNLV
                     NGVYKDEAIFQLINHQL"
     gene            complement(821..1603)
                     /gene="dapF"
                     /locus_tag="PLQ70_11860"
     CDS             complement(821..1603)
                     /gene="dapF"
                     /locus_tag="PLQ70_11860"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQE35356.1"
                     /translation="MQLEFYKYQGTGNDFVMIDNRSGFFPKENVQLVARLCDRRFGIG
                     ADGLILLENDTNTDFRMVYYNSDGNPSSMCGNGGRCLVAFAKDLSVIKDSCTFIATDG
                     LHHASVANDGIVSLQMIDVADIKKEKDYTFMNTGSPHHVQMVADLEHYNVKENGAALR
                     YGSLYGAAGSNINFVKKINEDTFSLRTYERGVEDETLACGTGATAVAIAMNATGQTNA
                     SSINLNVEGGKLAVSFDKMGDVFTNVFLIGPAKFVFKGVIEI"
     gene            1739..>2117
                     /locus_tag="PLQ70_11865"
     CDS             1739..>2117
                     /locus_tag="PLQ70_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008467953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HQE35357.1"
                     /translation="MKRFSSLFLVSLLSGAITLGSYKLLFDDNGYFSKNNSIVTQASN
                     SYERNVGLSAEAIDFTEAADKTIHTVVHVKNVSKTIVTNPMLEYFYGFRGGQEQEQVG
                     TGSGVIISEYGYIVTNNHVIKDAS"
BASE COUNT          669 a          410 c          351 g          687 t
ORIGIN      
        1 gggatagctt cttttattgg ctaccatttc aaaacgatcc atattatcaa caaagggttc
       61 aattataatc ttgacctcat cataagtagc acctgttggc acaaacacaa acacttcgtt
      121 ttgtgaaaat ttagtgtttt tagaaaaaat ttgattagcc aatattccgc cataaattag
      181 taatcctgtt attactacta aagttgctat cgttatgatt ttttttaaat tcaaaattga
      241 aattttttaa agttgatgat tgattaattg aaaaatagcc tcgtctttat aaacgccatt
      301 aactaaattc caatcttttt ttgtgcctat tttttggaat ccaaatttag taaaaagagc
      361 tatactagct ttattttctg tgctaatatt tgcataaaat tgatgtagat tgagatgata
      421 aaatccatat cgaattaaca actccaaagc ttcagaacca atattttgtc ttcgattttc
      481 ttccccttga ataacgattc ctattcctgc tctattattc tttgggtcaa aatcaaacaa
      541 atcgattaga cctagtgctg gaaaatcttc atcctgacaa atggccaatc gcaattgctt
      601 ggcttcatag atgtcttgat gggcattttc gagatattgc tttaccaaaa accgactgta
      661 aggcgtttgc gtattgctga cttcccaaat actttggtcg ttttccatgg catacacaaa
      721 ctctaaatca ttgggttcga gagctcggag atagatattt tcgcctttta gtgtgatcat
      781 ttaaaagtta gaggttagaa attagaggtt agaagtaaat ttatatctca atcactcctt
      841 taaaaacaaa tttcgctgga ccaattagga aaacattggt aaaaacatct cccattttat
      901 caaaagaaac ggctaatttc ccgccttcaa cattcaaatt aatggaagaa gcattcgtct
      961 gtcccgtggc attcattgca atcgcaaccg ccgtggctcc agttccgcat gccaaagttt
     1021 catcctccac acctctttcg taagttcgaa gcgaaaaagt atcctcgttt atttttttta
     1081 cgaaattgat gttgcttcct gctgccccat ataacgaacc gtaacgaaga gctgctccgt
     1141 tttcttttac attatagtgt tccaaatcgg ccaccatttg cacgtgatgc ggcgaaccgg
     1201 tattcataaa agtataatct ttctcttttt tgatatcggc aacatcaatc atttgcaaag
     1261 aaacaattcc gtcattggca acactggcgt gatgcaaacc atcggtagcg ataaaagtac
     1321 aactgtcttt aataacgctc aaatctttgg cgaaagccac caaacaacgg ccaccattgc
     1381 cacacatcga acttgggttt ccatcggaat tgtaataaac cattctaaaa tcggtgttgg
     1441 tgtcgttttc caacaaaatc aatccatcgg cgccaatgcc aaaacgtctg tcacacaaac
     1501 gcgccacgag ttgaacattt tcctttggaa aaaaccctga gcgattgtca atcatcacaa
     1561 aatcgtttcc tgttccttgg tatttataga attctaattg catttttttt gtttgagaag
     1621 tacaaaagta caaatattaa tgatacgtta aagattgtta accgagcgtt aaactgtttt
     1681 tttgaattga cataatttat aattttacaa ctataattct taaataacta taaaaactat
     1741 gaaacgattt tcaagcttat ttttagtgtc attgttgagc ggagccataa cattgggttc
     1801 ttacaaattg ttatttgacg acaatggata tttttctaaa aacaattcaa tagtcaccca
     1861 agcgtcgaat tcgtatgaaa gaaatgtagg tttatcagcc gaagctattg actttaccga
     1921 agcggcagac aaaaccattc acaccgtagt tcacgtaaaa aatgtctcca aaacaattgt
     1981 cacgaatcct atgctggaat atttctatgg ctttaggggc ggacaagagc aggaacaggt
     2041 aggaacgggt tcgggagtaa ttatttcgga atatggttat attgtaacca acaatcacgt
     2101 gataaaagat gcttctg
//
LOCUS       DAOSNN010000321         2079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_53606_length_2079_cov_6.448617, whole genome shotgun sequence.
ACCESSION   DAOSNN010000321 DAOSNN010000000
VERSION     DAOSNN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53606_length_2079_cov_6.448617"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PLQ70_11870"
     CDS             complement(<1..1488)
                     /locus_tag="PLQ70_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQE35358.1"
                     /translation="MNLKTSFLGLLLMAIPFFSNAQQSVNPNAFPVQITTANGIIEGE
                     FDIKTNIQSFKGIPFAQPPVGNLRWKAPQPVTNWTGIKQTKKFGPRAVQSNIFGDMSH
                     RSDGISEDCLYLNVWSPAKSSDEKLPVLVYFYGGGFAAGDGSENRYDGENMAKKGIVT
                     LTVNYRLGIFGFFSHPELTKESPNHASGNYGFLDQNAALKWVQANISKFGGDPKRVTI
                     AGESAGSISVSVQMASPLSKNLVAGAIGESGASIFPTLAPIPLAEAEKTGLEFGQKIG
                     VKTLKDLRSMSTLELYQKSMGTSLGVFKSTIDGYFMPKSLPEIFESKQQAMVPLLLGW
                     NSEEMTYLALTMGKGLTNESYAAKIKELYGDKAEEVLKLYPAGTPEVTEQSATDLAGD
                     RFIAYSTWKWFDLHRKNSSQPVYRYYFSKPRPEMRDKDLEAGLAGGVIKKDKNAPKAP
                     KPKGAVHSAEIEYAMGNLATNIDYAWTEDDYKVSETMMNYFANFIK"
     gene            complement(1524..>2079)
                     /locus_tag="PLQ70_11875"
     CDS             complement(1524..>2079)
                     /locus_tag="PLQ70_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQE35359.1"
                     /translation="GLREVSNPSEILISHKDEELSGTAIATTLEGMRPLMIEIQSLVS
                     TAVYGTPQRSTTGYNAKRLNMILAVLEKRAGFRLGAKDVFLNVTGGISVDDPAIDLAV
                     VAAILSSNEDIPVNKDFCFAGEVGLSGEIRPVNRVDQRIQEAEKLGFSTIFVSKYNKI
                     ALKNTIIKIRLVAKIEDVASELFG"
BASE COUNT          588 a          443 c          400 g          648 t
ORIGIN      
        1 tttaataaag ttggcaaagt aattcatcat agtttccgaa actttataat cgtcttctgt
       61 ccaagcataa tctatatttg ttgccagatt tcccatcgca tattcaattt cagccgagtg
      121 aacagcgcct tttggtttgg gcgcttttgg cgcattttta tcttttttga taacaccacc
      181 agcaagacca gcctccaaat ctttgtctcg catttcgggt ctaggtttcg aaaagtaata
      241 cctgtaaacg ggttgtgaac tgttttttcg atgcaaatca aaccatttcc aagtgctgta
      301 agcaataaat cggtcgccag ccaaatcggt cgccgattgt tccgtaactt caggagttcc
      361 tgcgggatat agtttcaaga cttcttcggc tttatcgcca tataattcct tgatttttgc
      421 agcataactt tcgtttgtga ggccttttcc cattgttaaa gcgagatagg tcatttcttc
      481 tgaattccat cccaataata aaggaaccat ggcttgttgt ttggattcga aaatttcggg
      541 taaagatttt ggcataaaat aaccgtcaat tgttgattta aaaacgccca aacttgttcc
      601 cattgatttt tgataaagct ctaaagtgga catagaacgt aagtccttca atgttttcac
      661 tccaattttc tgcccaaatt caagacctgt tttttcggct tcagccaatg gaattggagc
      721 taaagttgga aatatggacg caccgctttc accaatagca ccagcaacaa gatttttgga
      781 taaaggtgaa gccatttgaa cacttacgga aatggaaccc gccgattcac cagcgatagt
      841 tacgcgtttt gggtcaccac caaattttga aatattggct tgaacccatt tcaaagcagc
      901 attttgatcc agaaaaccat aatttcccga agcgtgatta ggagattcct tagtcaattc
      961 tggatgagag aaaaaaccaa aaattcccaa acgataattc acggttaggg taacgattcc
     1021 ttttttggcc atattttcgc catcataacg attttccgaa ccatcgccag cagcaaaacc
     1081 acctccataa aaatatacca aaacgggaag tttttcgtca gaagatttgg ccggagacca
     1141 aacgttgagg tataaacaat cttcgctgat accgtcgctt ctatgactca tatcgccaaa
     1201 aatgttggat tgtacggctc gtgggccaaa ttttttggtt tgtttgattc ctgtccaatt
     1261 cgtgacaggt tgaggtgctt tccaacgcaa attcccaacg ggtggttgag caaatggaat
     1321 gcctttaaaa ctttggatgt tggttttaat gtcgaattcg ccttcgataa taccatttgc
     1381 ggtggtgatt tgaacaggaa aagcatttgg atttacagac tgttgcgcat tcgaaaagaa
     1441 tggaattgcc atcaatagca atccaagaaa tgaggttttg agattcataa tttatgtttt
     1501 atagtgtgtt ttaatatttt tttctaccca aataattcgc tcgcaacatc ttcaatcttc
     1561 gcaaccaatc ttattttgat tatcgtgttt ttcaaagcaa ttttattgta tttggataca
     1621 aaaatggtcg aaaatcctag tttttcggct tcttgaatgc gttgatcgac acgattaaca
     1681 gggcgaattt cacccgaaag tcccacttcg cccgcaaaac aaaaatcttt gttcactgga
     1741 atatcttcat tcgaggataa aatggcggca acaactgcca aatcaatcgc aggatcatcc
     1801 accgagattc cgcctgtaac attcaggaaa acgtctttgg ctcccaaacg aaagccagca
     1861 cgtttttcta atacggccaa aatcatattc aacctttttg cgttgtaacc cgtggtgctg
     1921 cgttgcggtg ttccataaac tgccgtgctg accaaggatt gaatctcgat catcaacgga
     1981 cgcattcctt cgagagttgt agctattgct gtccccgaaa gttcttcgtc tttgtgcgaa
     2041 atcaatatct cggaagggtt cgaaacttct cgtaaaccg
//
LOCUS       DAOSNN010000322         2066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_54086_length_2066_cov_5.897066, whole genome shotgun sequence.
ACCESSION   DAOSNN010000322 DAOSNN010000000
VERSION     DAOSNN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54086_length_2066_cov_5.897066"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..>2066
                     /locus_tag="PLQ70_11880"
     CDS             232..>2066
                     /locus_tag="PLQ70_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HQE35360.1"
                     /translation="MKKKIEILAPAKDLIGGMAAINAGADAVYIGAPLYGARSNATNS
                     IEDVAKLVEYAHLFNVQVFVVVNTILYDNELEDCRQMIWKLYDVGVDALIVQDMAIME
                     MELPPIVLHASTQANNRDADKIKFLKDAGMKRVVLARELNLHQIKEISDTADVELEFF
                     VTGALCVSFSGNCYMSVANGERSANRGSCAQNCRLPYNLIDGHGETLIKNSHLLSIKD
                     FDVSDQIPNLVEAGICSFKIEGRLKDVVYVKNNVSYLRQKLDAFLEGAGSEKYAKASS
                     GTCTYTFESALDKSFNRGYTDYFVNERHQSIGSWESPKSKGQYIGKLIKTTGNAYEIE
                     NGEFLNNGDGLCFINENNEAEGIYVNKVENGLAYPNVLKEIKPGTFIYRNNDAAFIKI
                     VEREDSAVRKISTTLLLTENENGFELIATDEDGNVSIVKLVHPKERTKNNESIADNFK
                     VNLAKTGFTPYTADEITIMFSENWFLPISKINEMRRTVFEQLSEIRLANYNRHEHQLV
                     KTSHPYPETKLDFMYNVSNKLARKFYERHGVTEIEKAFELQWDPGKSRVMTTKYCIKY
                     ELEKCPKYHPNMDGRLKEPLVLKQGELEYKLKFNCKPCEMEIWEK"
BASE COUNT          692 a          442 c          419 g          513 t
ORIGIN      
        1 gcgtgaccac aagggtcggg ctattcgttg caatcttttc ctttttaaag aaaaaagaaa
       61 aaggatttcc actgctatcc ctcacgcaaa ataggatatc aagaaacaat tttccaaaaa
      121 atccattccc taattagaaa tcttgaataa tttataagac ctttgcaaac tttttggcta
      181 aaaccgagaa aaacctaaac cattaaaaac aaaacaatta tcattcattt gatgaagaaa
      241 aaaatagaaa tactcgcacc tgcaaaagat ttaatcgggg gaatggccgc catcaatgcc
      301 ggtgccgatg ccgtttatat tggagctcct ttatacggcg ctcgttccaa tgccaccaac
      361 tcgattgagg acgtggcgaa attggtggag tacgcccatt tattcaacgt gcaagttttt
      421 gtggtggtca acaccatttt gtacgacaac gaattggaag actgtcgcca gatgatttgg
      481 aaattgtatg acgtaggtgt cgatgccctg atagttcagg atatggcaat tatggaaatg
      541 gagttgccgc ctatcgtttt gcacgccagc acccaagcca acaatcgtga tgccgacaaa
      601 atcaaattcc tgaaagatgc cggaatgaaa cgtgtggttt tggctcgtga attgaacttg
      661 caccaaatca aggaaatcag cgacactgcc gatgtggaac tggaattttt cgtaacgggt
      721 gctttgtgcg tatctttcag tgggaattgt tatatgagtg tggccaatgg cgaacgctct
      781 gccaaccgcg gttcctgtgc ccaaaactgt cgtttacctt acaacctcat cgacggtcac
      841 ggcgaaacct taatcaaaaa cagccacctg ctttccatca aggattttga cgtttcggac
      901 cagattccaa acttggttga agctggaatt tgctctttca aaatcgaagg acgtttgaaa
      961 gatgttgtct atgtaaaaaa caatgtttcc tatttgcgcc aaaaactgga cgcttttctg
     1021 gaaggagcag gcagtgaaaa atacgccaaa gcctcttcag gaacctgtac ttatactttc
     1081 gaatcggctt tggacaaaag tttcaaccgc ggttataccg attattttgt caacgaaaga
     1141 caccaatcca tcggttcctg ggaaagtcca aaatccaaag ggcaatacat tggaaaattg
     1201 attaaaacca ctggaaacgc ttacgaaata gaaaacggtg aattcctgaa caatggcgac
     1261 ggattgtgtt tcatcaacga aaacaacgaa gccgaaggaa tttatgtcaa caaagtcgaa
     1321 aacggactgg cgtatccaaa tgtgttgaaa gaaatcaaac ccggaacttt catttaccgc
     1381 aacaacgacg ctgctttcat caaaatcgtg gagcgtgaag acagtgccgt tcgaaaaatc
     1441 agcacgactt tattgctgac cgaaaacgaa aacggttttg aactcatcgc caccgacgaa
     1501 gacggaaacg tgagcatcgt aaaattggtt catccaaaag aaagaaccaa aaacaacgaa
     1561 tcgattgcgg ataatttcaa agttaatttg gcaaaaacag gtttcacgcc ttacaccgcc
     1621 gatgaaataa caattatgtt ctccgaaaat tggttccttc ccatttccaa aatcaatgaa
     1681 atgcgaagaa cggttttcga acaattatcc gaaattcgtc tcgccaatta caatcgccac
     1741 gaacaccaat tggtcaaaac atcacatcct tatccagaaa ccaaactgga ttttatgtac
     1801 aatgtttcga acaaattggc ccgtaaattc tatgaacgtc acggcgttac cgaaatagaa
     1861 aaagcattcg aattgcaatg ggatcctgga aaatcacgtg tgatgaccac caaatactgc
     1921 atcaaatacg aattggaaaa atgcccgaaa taccatccca atatggatgg cagactcaaa
     1981 gaacctttgg tattgaaaca gggcgaactg gaatacaaac tcaaattcaa ttgcaaaccc
     2041 tgcgaaatgg aaatttggga aaaaga
//
LOCUS       DAOSNN010000323         1990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_56918_length_1990_cov_4.004651, whole genome shotgun sequence.
ACCESSION   DAOSNN010000323 DAOSNN010000000
VERSION     DAOSNN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56918_length_1990_cov_4.004651"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="PLQ70_11885"
     CDS             <1..101
                     /locus_tag="PLQ70_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012022971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HQE35361.1"
                     /translation="FKGPLTIMDVDTKEFWQSEPLPNLYPNEKGRN"
     gene            132..617
                     /locus_tag="PLQ70_11890"
     CDS             132..617
                     /locus_tag="PLQ70_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35362.1"
                     /translation="MKKIFTIVLLGAICMVNAQAFKGKGDTKLNIGMTLQDGGSGILA
                     IADFGLGENFSIGVLTSYLLGGSHIQDVPAKYRFDVKARFNANLGSVMNVSPKFDLYP
                     GLNLGLKNFGGHLGARYFFSDGFGVFTEFSVPFAKYDSDAISKYNNGSSFNIGASFNL
                     D"
     gene            complement(681..>1990)
                     /locus_tag="PLQ70_11895"
     CDS             complement(681..>1990)
                     /locus_tag="PLQ70_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012023307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQE35363.1"
                     /translation="RGEMVRQFGDKLRKNKEALGKLVSYEMGKSLQEGYGEVQEMIDI
                     CDFAVGLSRQLHGLTMHSERLNHRMYEQYHPLGIVGIISAFNFPVAVWSWNTALAWIC
                     GDVCVWKPSEKTPLCGIACQNIIAEVIKENNLPEGISCLINGDYKVGELMTIDKRIPL
                     ISATGSTRMGKIVAQNVAARLGKSLLELGGNNAIIVTPDADIKMTVIGAVFGAVGTAG
                     QRCTSTRRLIIHESIYDKLKEALARAYGQLKIGNPLDEKNHVGPLIDWQAVEMYSKAL
                     EKVVAEGGKIIVPGGVLSGHDYQSGCYVKPAIAEAENSFEIVQHETFAPILYLLKYSG
                     SVENAIKIQNGVVQGLSSAIMTNNLREAELFLSAMGSDCGIANVNIGTSGAEIGGAFG
                     GEKETGGGRESGSDAWKAYMRRQTNTINYGTQLPLTQGIKFDL"
BASE COUNT          571 a          414 c          356 g          649 t
ORIGIN      
        1 cttttaaagg gccattaacc ataatggatg tagacacaaa agagttctgg caaagtgaac
       61 ctttacccaa tttatatcca aatgaaaaag ggaggaatta aaatacttat ctttgcaaaa
      121 aaaatataat tatgaaaaaa atttttacaa tcgtattatt aggtgcaatc tgtatggtaa
      181 atgcacaagc ttttaaagga aaaggagata caaaactcaa catcggtatg accttacaag
      241 atggaggttc tggaatttta gccatagccg attttggact gggcgaaaac ttttcgattg
      301 gtgttcttac ctcctattta ttgggcggaa gccatattca agacgttcct gccaaatatc
      361 gctttgatgt taaagcaaga tttaatgcta atttgggaag tgttatgaat gtttctccaa
      421 aatttgattt gtatcctggt ttgaatttag gtttgaaaaa ctttggtgga catcttggcg
      481 caaggtattt cttctcggat ggatttggag ttttcactga attttctgtg ccgtttgcaa
      541 agtatgattc agatgctatt tcaaaataca acaatggctc atcgtttaat attggagctt
      601 cgttcaattt agactagtca attcaaaata aaaaagcggc tcgatgaaaa tcttgccgct
      661 ttttttatgg atttttttct ttacaaatca aattttattc cttgtgtcaa tggtaattgc
      721 gttccgtaat taatggtgtt tgtttgtctt cgcatataag ctttccaggc atcggagccg
      781 gattctcgtc cgccacccgt ttctttttcg ccaccaaaag ctcctccaat ttctgctccg
      841 gatgttccaa tattgacatt ggcaatgcca caatctgaac ccatcgctga caaaaataat
      901 tcggcttcac gcaaattgtt ggtcataatc gccgacgata atccttgcac aacgccgttc
      961 tgaattttga tggcattttc taccgaaccc gaatatttca aaagatataa aataggtgca
     1021 aaagtttcgt gttgcacaat ttcgaatgaa ttctcagcct ctgcaatggc aggttttaca
     1081 tagcaaccgc tttgataatc atggcccgag agcactcctc ctggcactat aatttttcca
     1141 ccttcggcaa caactttttc taatgctttt gagtacatct cgacagcctg ccaatcaatt
     1201 aatggtccca cgtgattttt ttcgtccaat ggatttccta tttttaattg gccgtaagcc
     1261 cgagccaaag cttcttttag tttgtcataa atactttcgt gaataatcaa tcttcgggtt
     1321 gaagtgcaac gctgtcctgc tgttccaaca gctccaaaaa cggctccgat tactgtcatt
     1381 tttatatcgg catctggtgt tacaataatg gcgttgtttc cgcccaattc cagtaatgat
     1441 tttcctaaac gagctgccac attttgcgcc acaattttcc ccattcgagt agaacctgtg
     1501 gccgaaatta atggaattcg tttatcgata gtcattaatt ctcccacttt ataatcgcca
     1561 ttgataagac aagaaatccc ttctggaaga ttattttctt taatcacttc ggcaataata
     1621 ttttgacaag caattccgca aagaggtgtt ttttcggatg gtttccaaac gcaaacatcg
     1681 ccacaaatcc aagccaaagc cgtgttccag gaccaaactg caacaggaaa attaaaagcc
     1741 gaaataatgc caacaattcc caagggatga tattgttcat acattcggtg attaagacgt
     1801 tcggagtgca ttgttaagcc gtgtaattga cgtgaaagtc ccaccgcaaa atcgcagatg
     1861 tctatcattt cctgaacttc gccgtagcct tcttgcaatg attttcccat ttcataggaa
     1921 actaattttc ctaaagcttc tttatttttt cgtaatttat ctccaaactg acgcaccatt
     1981 tcgcctcgtt
//
LOCUS       DAOSNN010000324         1969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_57777_length_1969_cov_4.380355, whole genome shotgun sequence.
ACCESSION   DAOSNN010000324 DAOSNN010000000
VERSION     DAOSNN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57777_length_1969_cov_4.380355"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLQ70_11900"
     CDS             <1..417
                     /locus_tag="PLQ70_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HQE35364.1"
                     /translation="FEEPLHHENFDGYQTLKNQTGISLAMGEREFNTQPLIELIKRKA
                     IDIWQPDILRIGGVEAWRESASIAAGFHIPVLPHYYKDYDVPLLCTIPNGVGAESFDW
                     IDDYIDNPMLVKDGFAYPHQQPGWGFNFIDEYLKKI"
     gene            421..1182
                     /locus_tag="PLQ70_11905"
     CDS             421..1182
                     /locus_tag="PLQ70_11905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010134253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQE35365.1"
                     /translation="MGQEAFSLEGKTALITGGGTGIGLGIAKEFINAKAKVIITGRRE
                     DVLKNACEALGENAHYVVADLSLLDTIPALVTDIESEFGSINILVNNAGIHLKKWAQE
                     TTDEEFLKIIQTNLLSVFALTREVAKGMLERKRGSIILISSMAGLFGIDRVSAYGTSK
                     TALIGLMNNLVTEYSRDNVRINCIAPGWINSDMFLNAINQDQERKQKITNRIALPDFG
                     KPEDIGNAAVYLSSQAGRYVTGVVLPVDGGATVNF"
     gene            1433..>1969
                     /locus_tag="PLQ70_11910"
     CDS             1433..>1969
                     /locus_tag="PLQ70_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007811806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HQE35366.1"
                     /translation="MSSKSLLFSILTLIATSSIFAQNPIVKIDFDHINQPTAQVTEPE
                     YTPWVVSTSPSSFTTNGVTFKVTRQGLKGDALGTNWYKAGIQSPYYAKLVCDGLTVKG
                     TTANEGGQIELRISGLATGTHTLLAFLNVTDSETFTYSPIDISVNGNLVLDNVIPSVK
                     AVKTADAKSVYLNLQATAG"
BASE COUNT          633 a          357 c          397 g          582 t
ORIGIN      
        1 tttgaagaac cattacatca cgaaaacttt gatgggtatc aaactttgaa aaatcaaacg
       61 ggaatttctt tggcaatggg cgaaagagaa ttcaacaccc aaccgctaat cgaattaatc
      121 aaacgcaagg ccatcgatat ttggcaaccc gatattttaa gaattggcgg tgtcgaagct
      181 tggagagaaa gcgcgtccat tgcggcagga tttcatattc cagtattgcc ccattattac
      241 aaagattatg acgtgccgtt gttgtgcacg attcccaatg gcgttggtgc ggaatctttc
      301 gattggattg atgattatat tgataatccg atgttggtaa aagatggttt tgcttatcca
      361 caccaacaac cgggctgggg atttaatttt attgacgaat atttgaaaaa aatataattt
      421 atgggacaag aagctttttc actcgaagga aaaacagcac tcattacggg tggcggaact
      481 ggaattggtt tgggcattgc caaagaattc atcaatgcca aagccaaagt aatcatcacg
      541 ggacgccgag aagacgtctt gaaaaatgcc tgtgaagcat tgggcgaaaa tgcccattat
      601 gttgtggccg atttgagcct acttgatact attccagcat tggtaacaga catcgaatct
      661 gaatttgggt cgattaacat cttggtgaac aatgccggaa tccatttgaa aaaatgggca
      721 caagaaacca ccgatgaaga atttttgaaa atcattcaaa ccaatttatt gagtgttttt
      781 gcattgactc gtgaagtggc gaaaggaatg ctggaaagaa aaaggggttc cattattctg
      841 attagttcga tggcgggatt gtttggtatt gaccgggttt cggcatacgg aacttcgaaa
      901 actgctttaa ttggcttgat gaacaacttg gttaccgaat attctcgtga caatgtgaga
      961 atcaattgca ttgctccggg ttggattaat tcggatatgt ttttgaatgc catcaaccaa
     1021 gaccaggaac gcaaacaaaa aatcaccaat cgaatcgcct tgcccgactt tgggaaaccc
     1081 gaagatattg gcaatgcagc ggtttatctt agctcgcaag ccggtcgata tgtaactgga
     1141 gttgttttgc ccgttgatgg tggcgcaacg gttaattttt gattggataa gctacaaatc
     1201 tccagaaaaa atgtcatgtt ctaattttcc aaggggatgc tgataaaaag tttacaacag
     1261 cttattaaat aattatttaa tacaatacgc ccttgttttt taatcaaata caccctgtat
     1321 aatcaggaca gtacaggata gttttgttac agcatttaat caaatttaca atcttgattt
     1381 tttctaatct atagaaaatt tcttcgtaaa tattaatcca aattataatt agatgtcctc
     1441 caaatcctta ttgttttcaa ttttaaccct aatagcaaca tcatctattt tcgctcagaa
     1501 tccaatagta aaaattgatt ttgaccatat aaatcagcca actgcccaag taacagaacc
     1561 tgaatatact ccctgggttg tttcaacatc tccaagtagt tttacaacaa atggagtcac
     1621 atttaaagtc actagacaag gacttaaagg cgatgcattg ggaacaaact ggtataaggc
     1681 aggtattcaa tctccttact acgccaaatt agtatgcgat ggccttactg taaaaggcac
     1741 aacagcaaat gaaggcggtc aaatagaact tcgaattagt ggtttagcca ctggtacgca
     1801 tactttgctt gcttttttaa atgtaacaga tagcgaaaca tttacctata gccccattga
     1861 tatttctgtg aatggtaact tagttctaga taatgtaatt ccaagcgtaa aggcagtaaa
     1921 aactgctgat gcaaaatcag tttatttaaa tttacaagct actgcagga
//
LOCUS       DAOSNN010000325         1933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_59321_length_1933_cov_5.052716, whole genome shotgun sequence.
ACCESSION   DAOSNN010000325 DAOSNN010000000
VERSION     DAOSNN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59321_length_1933_cov_5.052716"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLQ70_11915"
     CDS             complement(<1..362)
                     /locus_tag="PLQ70_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQE35367.1"
                     /translation="MEINLQIEKAKKGDQVAFTFLLNFYWNEVYGFMLKRTENETNAE
                     DITIETFSKAFDKIASYNPEFQFNTWLIAIAKNVHIDLLRKKKASLFIEITDDEDQQA
                     YNIADTTPSAEDELITEQ"
     gene            complement(353..1459)
                     /locus_tag="PLQ70_11920"
     CDS             complement(353..1459)
                     /locus_tag="PLQ70_11920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQE35368.1"
                     /translation="MLLFTLYFFIVIVAIQLAYYLIVFAKFAFAKAQKATPKKISVSV
                     IVCAKNEADNVARYIPLLAEQDYHDYEIILIDDASSDTTLEIFEEFEKQYSNIRLVKV
                     RNNEAFWGNKKYALTLGIKSAKKDYLLFIDADCYPSSKDWITTMSSQFTMQKTIVLGY
                     GAYEKVAKSFLNKIIRFETLLTAVQYFSWTKIGKPYMGIGRNLAYKKEEFYNVNGFIS
                     HIQVRSGDDDLFINQVATSKNTTICYTPESFTYSQPKTTFKGWFNQKRRHVSTATLYK
                     PFDKIQLALFYVSQLLFFILAILLLAFQYQWIIVLSLVGFRYLVTWIILGFSAGKLKE
                     KDVMYWFPIIEIVLIFTQLNIFITNLFSKPVHWK"
     gene            complement(1636..>1933)
                     /locus_tag="PLQ70_11925"
     CDS             complement(1636..>1933)
                     /locus_tag="PLQ70_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HQE35369.1"
                     /translation="KNPILLKSDFEKIHQQFPEMKYYEVSETEVKVPAGWLIEQAGFK
                     GKRFGDAGVHKNQALVLVNYGNATGQEILDVSKTIQETIFKTFGIHIEAEVNVI"
BASE COUNT          605 a          332 c          341 g          655 t
ORIGIN      
        1 ttttgctcgg taatcaattc gtcttcggcc gatggagtgg tgtcggcaat gttgtaggct
       61 tgttggtctt cgtcgtcggt gatttcaata aaaagtgacg ctttcttttt gcgcaacaag
      121 tcgatatgaa catttttagc aatagcaatc agccaagtgt tgaactggaa ttcaggattg
      181 taactggcta ttttgtcaaa agctttcgag aaggtttcga tagttatgtc ttcggcattg
      241 gtttcgtttt cagtgcgttt gagcataaaa ccatagactt cattccaata aaaattcaat
      301 agaaaagtga aagccacttg atcgcctttt tttgcttttt ctatctgtag gtttatttcc
      361 aatgtacggg ttttgaaaaa agattggtga taaagatatt taattgcgtg aagataagca
      421 caatttcgat aataggaaac caatacatca catctttttc ttttaatttt cctgccgaaa
      481 atccgaggat aatccaagtg actaaatatc ggaagcccac aaggctcaag acgataatcc
      541 attggtattg gaacgccaat aaaaggatgg ctaaaatgaa aaacaacaat tgcgaaacat
      601 aaaaaagtgc caactgaatt ttgtcaaaag gtttgtataa agtggcagtc gaaacgtgtc
      661 ttcttttttg gttaaaccaa cctttgaatg ttgttttggg ttgcgaataa gtgaaacttt
      721 caggagtata acaaatagta gtgtttttgc tagttgccac ttgattgata aacaaatcgt
      781 catcgccaga acgtacttga atgtgactaa tgaagccgtt tacattataa aattcctctt
      841 ttttataggc caaattacga ccaattccca tataaggttt ccctattttt gtccaagaaa
      901 aatattgaac ggctgtaagc aaagtttcaa aacggatgat tttgtttaag aacgatttgg
      961 ctactttttc ataagcgcca taacccaaaa caatcgtttt ttgcatcgta aattgcgaac
     1021 tcattgtcgt aatccaatct ttggaagagg ggtagcaatc ggcatctata aacagcaaat
     1081 aatctttttt ggccgattta attcctaatg ttaaagcata ttttttgttg ccccaaaaag
     1141 cttcgttgtt tctaactttt accaatctaa tattggaata ttgtttctcg aattcttcaa
     1201 aaatttctaa agtagtatca cttgaagcat cgtctatcaa tataatttca tagtcgtgat
     1261 aatcctgctc tgccagcaat ggaatgtaac gagctacatt atcggcttca tttttggcgc
     1321 aaacaattac cgaaacagag attttttttg gtgttgcttt ttgggctttg gcgaaagcaa
     1381 atttcgcaaa aactatcaga taatacgcaa gttgtatggc aactataaca ataaaaaagt
     1441 ataaagtaaa gagtaacata taggcttggc ttcttttttt aaaatctgtg caaaggtaat
     1501 tatcaattgt taataaacca tcgataaacg gcaattatta taatctaaat ttcaatgtct
     1561 aaattccaaa ttctaaaatg gaaattggat ttctaaaatt atgctttctt ccttctaatt
     1621 tctgcctttt aaaggctaaa tcacattcac ttcggcttca atatgaatgc caaaagtttt
     1681 gaaaatagtc tcttggatgg ttttagaaac atccaaaatt tcttgtccgg ttgcgttgcc
     1741 ataattcacc aaaactaaag cttgattctt gtgaactcct gcatcgccaa aacgttttcc
     1801 tttgaaacca gcttgctcaa tgagccaacc cgcaggaact ttgacttcgg tttccgatac
     1861 ttcatagtat ttcatttctg ggaattgttg gtgaattttc tcgaaatcag attttaaaag
     1921 aatcggattt tta
//
LOCUS       DAOSNN010000326         1914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_60144_length_1914_cov_4.588488, whole genome shotgun sequence.
ACCESSION   DAOSNN010000326 DAOSNN010000000
VERSION     DAOSNN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60144_length_1914_cov_4.588488"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /gene="hisF"
                     /locus_tag="PLQ70_11930"
     CDS             complement(<1..721)
                     /gene="hisF"
                     /locus_tag="PLQ70_11930"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQE35370.1"
                     /translation="MLTKRIIPCLDIKNGRTVKGVNFVDLRDAGDPVELAKIYSDEGA
                     DELVFLDISATEERRRTLVDLVRKVAATINIPFTVGGGISAVSDVEVLLQNGADKVSI
                     NSSAVKNPQLINDLAQKFGSQCVVVAIDAKQIDGEWMVHLVGGKVPTEIRLFDWAQEV
                     EQRGAGEILFTSMNHDGTKNGFANEALAKLSELVNIPIIASGGAGNMQHFVDTFVEGK
                     ADAALAASVFHFKEIEIKTLKK"
     gene            complement(792..1514)
                     /gene="hisA"
                     /locus_tag="PLQ70_11935"
     CDS             complement(792..1514)
                     /gene="hisA"
                     /locus_tag="PLQ70_11935"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HQE35371.1"
                     /translation="MRIIPAIDIIEGKCVRLSKGDYNTKIIYNENPLEVAKEFEAHGI
                     EYLHLVDLDGAKSSKIVNYKILEQIASQTKLKIDFGGGLKADSDLKIAFESGANQITG
                     GSIAVKNRAIFEKWIAEYGSDKIILGADANNEKVAVSGWLEDSNEDLVPFIQEYQNKG
                     IQYVICTDIAKDGMLEGPSFDLYEKILKQAEGLKLIASGGISTFDELPKLAELGCEGT
                     IIGKAIYEGRISLKQLEQYIIG"
     gene            complement(1522..>1914)
                     /gene="hisH"
                     /locus_tag="PLQ70_11940"
     CDS             complement(1522..>1914)
                     /gene="hisH"
                     /locus_tag="PLQ70_11940"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016989364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQE35372.1"
                     /translation="TLKQPVFGICLGMQLMCNSSEEGNTKGLGIFDVDVVKFTSKVKV
                     PQMGWNTIYNLKSDLFKGIAENEYMYLVHSFYAPLCAETIATTNYELEYSSALENDNF
                     YGTQFHPEKSGDIGEQILKNFLLLTSNF"
BASE COUNT          542 a          435 c          275 g          662 t
ORIGIN      
        1 ctttttttaa agttttgatt tcaatttctt tgaaatgaaa tacacttgcc gccaatgccg
       61 catcggcttt tccttcgaca aaggtatcta caaaatgttg catatttcca gcaccacccg
      121 aagcaattat cggaatattt actaattcag acaatttcgc caaagcttcg tttgcaaaac
      181 cgtttttggt tccgtcgtga ttcattgatg taaacaaaat ttcgcctgct ccacgttgct
      241 ccacttcttg tgcccaatcg aataatctga tttctgttgg cactttccct cctaccaaat
      301 gcaccatcca ttcgccatca atttgcttgg catcaatcgc taccaccaca cattgactac
      361 caaatttttg tgccaaatca ttaatcagct gtggattttt caccgcggat gaattaatag
      421 aaactttgtc cgcaccattt tgcaacaata cttccacatc tgaaactgcc gaaattccac
      481 caccaaccgt aaacggaata ttgattgttg cggccacttt tctaaccaaa tcaaccaagg
      541 ttcttcttct ttcttccgtt gccgaaatat caagaaaaac caattcatct gcaccttcat
      601 cggaatagat tttggccaat tccacaggat caccagcatc tcgcaaatct acgaaattaa
      661 cacctttaac ggttcgtccg tttttgatat cgaggcaagg gattattctt tttgttagca
      721 ttattttttt agttgttggt tgttggttgt tggttgttgg tcaatgttta caaactatca
      781 accgacaact atcaacctat tatatattgt tccaattgtt tcaacgaaat tcttccttca
      841 taaatcgctt tacctataat agttccttca cagcccaatt cagctaattt aggcaattca
      901 tcaaaagtgg aaattccccc tgaagcgatt aatttcaatc cttccgcttg ttttaaaatc
      961 ttttcataca aatcaaaact tggaccttcc aacattccgt cttttgcaat atcagtgcaa
     1021 attacgtatt gaattccttt attttgatac tcctgaataa acggcaccaa atcttcattc
     1081 gaatcttcta gccagcctga aaccgctact ttttcgttat tggcatcagc ccccaaaatg
     1141 attttatctg aaccatattc tgcaatccat ttttcaaaaa tagcacgatt ttttactgca
     1201 atgcttccac cagtaatttg attggcaccg ctttcaaaag caatttttaa atcagaatca
     1261 gctttcaatc cacctccaaa atcaattttc aatttcgttt gcgaagcaat ttgctccaat
     1321 attttataat tgacaatctt gcttgatttt gctccgtcca aatccaccaa atgcaaatat
     1381 tcgattccgt gggcttcgaa ttctttggct acttccagtg gattttcatt gtatatgatt
     1441 ttggtattgt aatcgccttt cgataagcga acacattttc cttcgatgat gtctattgca
     1501 ggtattattc tcattttaaa gttagaagtt agaagttaga agtaggaagt ttttcaggat
     1561 ttgttctcca atatccccac ttttttcagg gtgaaattga gttccataga aattatcatt
     1621 ttccaatgcc gatgaatatt ctagttcgta attggtcgta gcaatcgttt cggcacaaag
     1681 cggagcgtaa aaactatgta ccaaatacat atattcattc tcagcaattc ctttgaaaag
     1741 atcagatttg agattgtaaa tcgtgttcca tcccatttgt ggtactttca cttttgaagt
     1801 gaattttacc acatctacat caaaaatccc caatcctttt gtatttcctt cttccgaaga
     1861 attacacatc aactgcattc cgagacaaat tccaaaaacc ggttgtttca gcgt
//
LOCUS       DAOSNN010000327         1903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_60633_length_1903_cov_4.238095, whole genome shotgun sequence.
ACCESSION   DAOSNN010000327 DAOSNN010000000
VERSION     DAOSNN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60633_length_1903_cov_4.238095"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="PLQ70_11945"
     CDS             <1..1319
                     /locus_tag="PLQ70_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HQE35373.1"
                     /translation="ETKFDSKGSLAADGILGPHREKEGSYYTVKEIWAPIQFANKQVT
                     DAFDGSFLITNDYLFSNLNSCKMEYKVLKSDANILYTDAVSKTISTGTIEIPSIEPGE
                     ARKIKFALPANFFEGDVLSITANDIHGKEIYTWTWPIHKAQFFASKFLVSKATKAKAT
                     AVQTANEVTLKGSDISVTLDKTTGEILTIKNATSVIPLTNGPRPIGMKAKVSEITVAQ
                     EGENAVFSVSYIGGLSSIKWIMEPDGRFKMELVALKNAENAGGFDGAYFEDKISAFGI
                     TFSFPEKGVTGMKWFGKGPYHVWKNRLKGTTYGVWEKAYNTTITGESFENLVYPEFKG
                     YHANVIGANLKAGTNSFKVFSESENLFLRVFTPDLPKNGFPGSNPQPAFPEGDISFMY
                     EISAMRDFKPLEHQGPESQPTNIRIKKGDDGISMNLWFDFRVFTDK"
     gene            1340..>1903
                     /locus_tag="PLQ70_11950"
     CDS             1340..>1903
                     /locus_tag="PLQ70_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQE35374.1"
                     /translation="MKSIKILLALSLAFLFFNFTTKEQDKPTIYTVGDSTVKNGAGKG
                     DGGLWGWGDYIGQFLDITKVNLENHALGGTSSRTFQQKGLWEAVYKKLKKGDYVMIQF
                     GHNDDGPLNDDSRARGTIKGIGEETQEIDNMLTKKHEVVHSYGWYIQKVVREAKSKGA
                     IPIVCSPIPRNDWKEGKVPRNNKSYGLW"
BASE COUNT          640 a          365 c          410 g          488 t
ORIGIN      
        1 gagaaaccaa attcgactcc aaaggttcgc ttgcagccga cggaattttg ggacctcacc
       61 gtgaaaaaga aggcagctat tataccgtaa aagaaatttg ggcacccatc cagtttgcaa
      121 acaaacaagt aaccgatgct tttgatggtt cgtttttgat taccaacgac tatcttttca
      181 gtaatttgaa ttcctgcaaa atggaataca aagtactaaa atccgatgcg aatattcttt
      241 ataccgatgc cgtttcgaaa acaattagca ccggaactat cgaaattcca agcattgaac
      301 ccggagaagc tcgcaagatc aaattcgcac ttccagccaa ctttttcgaa ggcgatgttt
      361 tatccatcac ggcgaatgac attcacggaa aggaaattta tacctggact tggcctattc
      421 acaaagcaca gttttttgca tccaagtttt tagtttccaa agcaacgaaa gcaaaagcaa
      481 ctgctgttca aacggccaac gaagttacat taaaaggaag cgacatcagc gtaactttgg
      541 acaaaacaac gggagaaatc ttgactatca aaaatgcgac ttcagtaatt ccgttgacca
      601 atgggccacg tcctattggg atgaaagcca aagtaagtga aattacagta gcacaagaag
      661 gcgaaaatgc ggtgttttca gtttcctaca ttggtggttt atcgtccata aaatggatta
      721 tggaacctga cggaagattc aaaatggaat tagtagcctt gaaaaatgcc gaaaacgccg
      781 gtggttttga cggagcttat tttgaagaca aaatcagtgc gttcggaatc acgttcagct
      841 ttcccgaaaa aggagttacg ggaatgaaat ggttcggaaa agggccttat cacgtttgga
      901 aaaacagact caaaggaacg acttacggtg tttgggaaaa agcgtacaac acaaccataa
      961 caggcgaaag tttcgaaaat ttggtgtatc ccgaattcaa aggctatcac gccaatgtca
     1021 tcggagctaa tttgaaagct ggcacaaact cattcaaagt atttagcgaa tcagagaatt
     1081 tgtttctacg agttttcact cccgatttgc ctaaaaacgg atttccgggc agcaatccgc
     1141 aaccggcttt tccagaagga gatatttcct ttatgtatga aatttcggcg atgcgtgatt
     1201 tcaaaccatt ggagcaccaa gggccagaaa gtcagccgac caacatccga atcaaaaaag
     1261 gagacgacgg aatttcgatg aatctttggt tcgattttag agtttttaca gataaataaa
     1321 tttatatttt aacaccatta tgaagtcaat taaaatactt ttagctctta gtttagcttt
     1381 cttgtttttc aattttacaa ccaaagaaca agacaaaccc actatttata ccgtaggcga
     1441 ttcaaccgta aaaaatggag ctggaaaagg cgatggcgga ctttggggct ggggcgatta
     1501 tatcggacaa tttttggata ttacaaaagt taatctcgaa aaccacgctt tgggtggcac
     1561 cagcagccga acttttcagc aaaaagggtt gtgggaagca gtgtataaaa aacttaaaaa
     1621 aggcgattac gtgatgattc aatttggtca taatgacgat ggtccattaa atgatgattc
     1681 cagagccaga ggaaccatca aaggaattgg cgaagaaacc caagagattg ataatatgtt
     1741 gaccaagaaa cacgaagtgg tgcattctta tggttggtac atccagaaag tggttcgcga
     1801 agccaaatca aaaggagcga ttccgattgt gtgttctcct attccaagaa acgattggaa
     1861 agaaggaaaa gtgcctcgaa acaacaaatc ttacgggtta tgg
//
LOCUS       DAOSNN010000328         1828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_64197_length_1828_cov_3.706712, whole genome shotgun sequence.
ACCESSION   DAOSNN010000328 DAOSNN010000000
VERSION     DAOSNN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64197_length_1828_cov_3.706712"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLQ70_11955"
     CDS             complement(<1..744)
                     /locus_tag="PLQ70_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HQE35375.1"
                     /translation="MNFIQLKNRVAKKDLLVLSCLAIFFSANISVAQNTFPDIVKTKE
                     GKLERTVDAKGNQIPDFSFAGYKASSVAIPSVEIKVFVPHIDGDATQTIQSAIDYVAK
                     LKPDAAGFKGTVLLDKGIFKVSGVINIKESGIILRGSGVDKTILLGTSINREAIINIS
                     GINNLVFKEKFELADNFTPLGATVLAVKNGTSLKKGDHILINTPITKNWIDLLSMNDF
                     GGESSWIGWKSDDFVIRADREITAVQGNKI"
     gene            complement(852..>1828)
                     /locus_tag="PLQ70_11960"
     CDS             complement(852..>1828)
                     /locus_tag="PLQ70_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide lyase"
                     /protein_id="HQE35376.1"
                     /translation="PAVNVTIQNTISSEGLDTYNHAFGSTIGGLNSMFIRNLWADNIS
                     RNASVGMYGSFNFVNNVVFNWWNRTLDGGDYRSQYNIINNYFKPGPITPTDQPIRYRI
                     IKPESGYISPKQYGKAFVDGNFIVGSPEVTADNWNGGVQLEDLSPEITKDFLAQIKQN
                     KAFRMPPIAIMKAEQAYDFVLENAGATLPKRDAVDERIVKQVRTGKIEVKDGLENSIG
                     KEFIKRRLPADSYKRGIITNPNQVGGYPEYKGKAYKDSDNDGIPDAWETKYGLNPKDA
                     SDANKDLNGDGYTNIEKYINGIDPTKKTDWTKIENNTDTLAQLKALVQ"
BASE COUNT          503 a          362 c          304 g          659 t
ORIGIN      
        1 aattttattg ccttgaactg ctgtaatttc tctatcagca cgaatgacaa aatcatcaga
       61 tttccaacca atccagctac tttcacctcc aaaatcattc attgacaaaa gatcaatcca
      121 atttttggtg atgggcgtat tgattaggat gtgatctcct ttttttaggg aagttccatt
      181 ttttaccgct aaaactgtcg ctcctaatgg tgtgaagtta tcagctaatt caaatttttc
      241 tttaaaaacg agattgttga ttccgctgat gttgataatc gcttctctat tgattgatgt
      301 ccctaaaagt attgttttat caacgccact tcctcttaga ataattccgc tttccttgat
      361 attgataact cctgaaactt tgaagattcc tttgtctaaa aggactgttc ctttaaaacc
      421 agctgcatcg ggtttgagtt tagccacata atctatggcc gattgaatag tttgggttgc
      481 gtctccatcg atatgaggaa caaaaacttt gatttctaca gatggaattg ctaccgaaga
      541 agctttatat cctgcaaatg aaaaatcagg aatttggttt ccttttgcat caacagtacg
      601 ttccagtttt ccttctttgg ttttaacaat atcgggaaat gtgttttgtg ccacactaat
      661 attggcacta aagaaaatag caagacagga taaaactagt aaatcctttt ttgctactct
      721 atttttaagt tggataaaat tcacttcaat tttatttttg attttttaga atatggaaaa
      781 tagaataaag attatagatt tctaatgaac tctctctctt tctattttcc acaatctatt
      841 ttctctgctc tttactgtac taatgctttt aactgtgcca gcgtatcggt gttgttttca
      901 attttcgtcc aatccgtttt tttggttggg tcgattccgt taatatattt ctcgatattg
      961 gtataaccgt caccattcaa atctttgttg gcatcggagg catcttttgg attcaaccca
     1021 tatttagttt cccaagcatc aggaattccg tcattatccg aatctttata agctttccct
     1081 ttgtattctg gataaccgcc cacttgattt ggattggtta taattcctct tttataggaa
     1141 tcggcaggca atcttctttt gatgaattct ttgccaattg aattttccaa tccgtcttta
     1201 acttcaattt ttccagtacg aacctgttta acgattcttt catctactgc atcacgtttt
     1261 ggtaaagtcg ctcctgcatt ttctaataca aaatcgtaag cttgttccgc tttcataata
     1321 gcaattggcg gcattctaaa agctttattt tgtttaattt gtgccaaaaa gtctttggta
     1381 atttcaggcg acaaatcttc taattgcact ccaccattcc agttatcggc tgttacttct
     1441 ggcgaaccca caataaagtt tccgtcaaca aaggcttttc cgtactgttt tggactaata
     1501 taacctgatt cgggtttgat gattcggtaa cgaatcggct gatcggttgg cgtgattggt
     1561 cccggtttga aataattatt gataatgttg tattgcgaac gatagtctcc accatcaagg
     1621 gtacgattcc accaattgaa aacgacatta tttacaaagt taaagcttcc atacattcca
     1681 accgaggcat ttctcgaaat attatcggcc cataaattgc ggataaacat actattcaaa
     1741 ccaccgatag tacttccgaa agcgtgattg taagtatcca gaccttctga cgaaatggta
     1801 ttctgaatgg tcacattaac agccggga
//
LOCUS       DAOSNN010000329         1821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_64518_length_1821_cov_4.411665, whole genome shotgun sequence.
ACCESSION   DAOSNN010000329 DAOSNN010000000
VERSION     DAOSNN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64518_length_1821_cov_4.411665"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLQ70_11965"
     CDS             <1..245
                     /locus_tag="PLQ70_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQE35377.1"
                     /translation="MGEIMKEMSQTMQIFAITHLPQIAAKGTAHFKVSKSTVGEDTQS
                     ELRLLSNDERVVEIAQMLSGTTVSDSALNHAKALLN"
     gene            305..1495
                     /locus_tag="PLQ70_11970"
     CDS             305..1495
                     /locus_tag="PLQ70_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011963947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HQE35378.1"
                     /translation="MIIEPRMRGFICVTSHPDGCAQSVKNQIEYIKSKGTIDGAKKVL
                     VIGASTGFGLSSRITAAFGSDAATIGVFFEKPPLEGKPGSPGWYNSAAFETEANKAGL
                     YAKSVNGDAFSNEIKRQTLDLIKADLGQVDLVIYSLASPVRLHPVTGVLHRSVLKPIG
                     ATYTNKTVDFHTGVVTDISIAPCQDEDIANTVAVMGGEDWAMWMDALKAENLLAPGVK
                     TVAYSYIGPSLTEAVYRKGTIGRAKDHLEATAFSISESLKSVDGEAFVSVNKALVTQA
                     SSAIPVIPLYISLLYKIMKEEGIHEGCIEQIQRLFQDRLFTGKSIPTDNKGRIRIDDW
                     EMRDDVQAKVATLWKEAVTENLAEIGDLEGYRKDFLNLFGFDFEGVDYKADADEMVGI
                     PSIV"
BASE COUNT          607 a          276 c          370 g          568 t
ORIGIN      
        1 gaatggggga gattatgaaa gaaatgagtc agacgatgca aatatttgcc atcacgcatt
       61 tgcctcaaat tgcagccaaa ggaacggcac atttcaaagt ttctaaatca accgttggcg
      121 aggatacaca atcggaattg cgattattgt ccaatgatga acgtgtggtc gaaattgcgc
      181 aaatgttatc aggaactaca gtttcggatt ccgcattaaa ccacgcaaaa gccttgttga
      241 attaaagaaa tgacttatat ttgtcgccgt aaaaataccc aataactgaa aattttttgc
      301 cattatgatt atagaaccaa gaatgagagg atttatttgc gtaacctcgc acccagatgg
      361 atgtgcgcaa agtgtaaaaa atcaaattga atacatcaaa tccaaaggaa caattgatgg
      421 agcgaagaaa gttttagtaa ttggagcttc aacaggattt ggattgtctt caagaattac
      481 agctgctttt ggttcagatg ctgcaacaat tggtgtgttt tttgaaaaac ctccattaga
      541 aggaaaacca ggttctccag gttggtataa ttcagccgct tttgaaacag aagctaataa
      601 ggcaggtttg tatgccaaaa gtgtgaacgg agatgctttt tcgaatgaaa taaaaagaca
      661 aacattagat ttaatcaaag ccgatttagg tcaggttgat ttagtaattt atagtttggc
      721 ttctcccgtt cgtttgcacc cagttactgg agttttgcat cgttcggttt taaaaccaat
      781 tggagccact tacaccaata aaaccgtcga ttttcataca ggagttgtga ctgatattag
      841 cattgcacct tgccaagatg aagatattgc caatactgtt gccgtaatgg gtggcgagga
      901 ttgggctatg tggatggatg ctttgaaagc cgaaaattta cttgctccag gcgttaaaac
      961 ggttgcttat tcttatatcg ggccatcatt gactgaagcg gtttatagaa aaggaacaat
     1021 tggtcgtgcc aaagatcatc ttgaagccac agctttttcg atttcggaaa gcttgaagtc
     1081 tgttgatgga gaggcatttg tttcggtaaa taaagctttg gtaactcaag ctagttctgc
     1141 cattcctgtt attcctttgt atatttcctt gttgtataaa ataatgaaag aagaaggaat
     1201 tcacgaaggt tgtatcgagc aaattcaacg tttgtttcaa gacagattgt ttacaggcaa
     1261 atcaattcca accgataata aaggaagaat tagaattgac gattgggaaa tgcgtgatga
     1321 tgttcaagca aaagtggcta ctttatggaa agaagctgtt accgaaaatt tagctgaaat
     1381 tggcgattta gaagggtata gaaaagattt cttgaatctt tttggtttcg atttcgaagg
     1441 tgtagattat aaagcagatg ctgatgaaat ggtaggaatt cctagtatag tttaagaatt
     1501 aaattattat tttaaataaa gtaaaccatc aattaatagt tgatggtttt tatttataaa
     1561 aaaacctttc aaagtttaaa attctgaaag gtttttttgt ttattcttat ttgtaatcaa
     1621 agaagtaatt ctgcttaaaa agctcaaaaa aaatacggcc gcataaattt tcgggaatta
     1681 agcaaaaagt ttttactatt gggctacttt ttcaaactta tatgcacaac cttcatcaca
     1741 cattaaataa ctgggcgtta atattactgg atatgcaaat aaaaactaca cattaagtta
     1801 agctacattt ttgtaattaa t
//
LOCUS       DAOSNN010000330         1816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_64787_length_1816_cov_4.281090, whole genome shotgun sequence.
ACCESSION   DAOSNN010000330 DAOSNN010000000
VERSION     DAOSNN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64787_length_1816_cov_4.281090"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLQ70_11975"
     CDS             complement(<1..768)
                     /locus_tag="PLQ70_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQE35379.1"
                     /translation="MKLLKSLYINNFFFYALLGVVGLFVLAFIFPALYNATWYVVLML
                     LTFLVLDVLILFFTKNGIEGTRMMSEKLSNGDENEIRVGIKNYYTFPISVKIIDEIPF
                     QFQVRNFEIKQKIKASSEKEIQYYLRPTERGEYYFGNLNVYVSSPLFLISRRFQFDKE
                     RMVPTYPSYIQLRKYSLMAFSNNLFQYGIKKIRRIGHTMEFEQIKEYNQGDDIRTLNW
                     KATAKKNALMVNQFQDEKSQSVYMIIDKGRVMKMPFNG"
     gene            complement(919..1176)
                     /locus_tag="PLQ70_11980"
     CDS             complement(919..1176)
                     /locus_tag="PLQ70_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQE35380.1"
                     /translation="MKAKFIKISLIVISIFVIVLLYFNSSYYIEKQFWKYNAGKYIGD
                     VITNQKQIDNSNSQIVFCFGKKLIIEDLKTGENGYYENKSW"
     gene            complement(1211..>1816)
                     /locus_tag="PLQ70_11985"
     CDS             complement(1211..>1816)
                     /locus_tag="PLQ70_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQE35381.1"
                     /translation="KTQAALFEVMEERQITIDGTTYILDTPFLVIATQNPIEQEGTYR
                     LPEAQLDRFLFKISIDYPKMDEEILIIQREHLLQNQGKLDNIKTLLSSEEIKYYQALV
                     KQIIVEQNLLEYIAKIVINTRENAFLYLGASPRASIAILNAAKGFAAIRGRDFVTPED
                     IKEAAIPVLQHRVIVTPEREMEGITSIEIIKQILESVEIPR"
BASE COUNT          542 a          324 c          324 g          626 t
ORIGIN      
        1 tccgttgaaa ggcattttca tcacgcggcc tttgtcgata atcatataaa ccgattgtga
       61 tttttcgtcc tgaaattgat tgaccatcaa ggcatttttc ttggcggtag ctttccaatt
      121 caaggttcga atgtcgtcgc cttgattgta ttccttgatt tgttcaaatt ccattgtgtg
      181 accaattctt cggattttct tgatgccgta ttgaaacaaa ttgttggaaa aagccatcaa
      241 actgtatttt cgtaactgaa tgtaggatgg ataagtcggc accattcgct ccttgtcgaa
      301 ttgaaatcgt ctcgaaatta aaaacagggg agaggaaaca tacacgttca aattcccgaa
      361 ataatattcg ccacgttcgg taggacgcaa ataatattgg atttcttttt ccgaagaagc
      421 cttgattttt tgcttgattt caaaattgcg aacctgaaat tgaaaaggaa tttcgtcaat
      481 tattttcact gaaatcggaa aagtataata gtttttgata cccactctta tttcgttttc
      541 atctccatta gataattttt cggacatcat tcgagttcct tcgataccat ttttggtaaa
      601 aaacaaaatc aggacatcca acaccaaaaa agtcaacaac atcaatacaa cataccaagt
      661 ggcgttgtac aatgcgggaa atataaaggc caaaacaaac aatcccacca cgcccaaaag
      721 cgcatagaaa aagaaattgt tgatgtataa actttttagt agtttcataa tttttagaat
      781 ctgaaaactt aatttttagc cacggattta actgattaaa cggatttacg cagatctttt
      841 aatcaaactt ttttaatccg tttttatatg tgtgacattt tttttatgct ttgcaagtaa
      901 tgatacaatc tttttatatt accagctttt gttttcgtaa tatccatttt ctccagtttt
      961 taaatcttca ataattagtt tttttccaaa acagaataca atttgagagt tagaattatc
     1021 tatttgtttc tggtttgtaa tgacgtctcc aatatatttt ccagcattgt atttccaaaa
     1081 ttgtttctct atgtaataag aactattgaa gtataataat acaatcacaa aaattgatat
     1141 tacgattaaa gatattttaa tgaattttgc tttcatattt tttgaattct agaaaattta
     1201 catctaaaaa ctacctagga atctccacac tttccagtat ctgcttgata atttcaatac
     1261 tcgtaatgcc ttccatttcg cgttctggcg ttacgataac acggtgttgc aacacaggaa
     1321 tcgcagcttc tttgatgtct tccggcgtca cgaaatcacg tccacggatg gcggcaaaac
     1381 ctttggcggc attcaagatg gcgatggaag cacgaggcga agcaccgaga tacaaaaagg
     1441 cattttcacg agtgttgatt acgattttag caatgtattc caacaggttt tgttcaacga
     1501 taatttgttt gaccaaggct tgatagtact tgatttcctc cgaggaaagc aaggttttga
     1561 tgttgtcgag tttgccttgg ttttgaagta aatgttctct ttggataatc aggatttcct
     1621 cgtccatttt tggataatcg atggagattt tgaacagaaa acggtctaat tgcgcttccg
     1681 gcaggcgata agttccttct tgttctattg gattttgggt ggcaatgact agaaaaggcg
     1741 tatccaaaat ataggttgta ccatcgatgg tgatttggcg ttcttccatc acttcaaaaa
     1801 gggcggcttg ggtttt
//
LOCUS       DAOSNN010000331         1674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_72588_length_1674_cov_5.327363, whole genome shotgun sequence.
ACCESSION   DAOSNN010000331 DAOSNN010000000
VERSION     DAOSNN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72588_length_1674_cov_5.327363"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLQ70_11990"
     CDS             <1..701
                     /locus_tag="PLQ70_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012025468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HQE35382.1"
                     /translation="LNLIPYIPIEGFQADFLEFVKDGVPPNTYDAIYKIISDILNNSH
                     SGLLSSGFILSIFLMANGLNGILGGFESSKHVLIKRGFLHQYLVALGMSLVLSLLLLL
                     TVTIIIVFEVIIQMTIIQDVLSDRIPLIILGRYVFVILMILITISILFKFGTTHTQNS
                     AFISIGSVFTTVLVILDSYVFGIWVLRFSKYNELYGSIGTLLIMMFYIWINCAILLLG
                     FELNASINKLKQKK"
     gene            761..>1674
                     /locus_tag="PLQ70_11995"
     CDS             761..>1674
                     /locus_tag="PLQ70_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017496604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQE35383.1"
                     /translation="MHFVEVILPLSLDKTFTYSVSETEYNYIKIGMRLAVPFGKSRIY
                     TALVIAIHQNKPTLYDAKEIHQILDEKPIVTEIQITHWQWIASYYMCAIGDVYRGAMP
                     SALLLESETIISQKNDGFVDESLLTDDEFLIYEALQQQSSLKVQDIMSILNKKNIFPV
                     IQKLIDKNILVLQEEMLETYTPKLIRYVKLHSKYETNEGLSELLEVLKNANKQKEIVL
                     SYFQLSASEKKPITVKKLIEVANSTSTIVKTLIDKEIFEEYYIQVDRVNFIGKTKDEQ
                     LQLSEVQQIAFEEIKENFTQKEVCLLHGV"
BASE COUNT          560 a          255 c          286 g          573 t
ORIGIN      
        1 ttttaaacct cattccgtat attcctatcg aaggatttca ggccgatttc cttgagtttg
       61 tcaaggatgg agttccaccc aatacctacg atgctattta taaaatcatt agcgatattc
      121 tcaataatag tcattcaggc ttactttctt ctggttttat attgtctatt ttcttgatgg
      181 caaatggttt aaatggaatt ttaggtggtt ttgaatcttc gaagcatgtg cttatcaaaa
      241 gaggtttttt gcatcaatat ttggttgctt tgggaatgtc tttagttttg tcgttattgt
      301 tattgctaac agttaccata attattgttt ttgaagttat tattcaaatg accatcattc
      361 aagatgtgtt gagcgatagg attcccctaa ttattttagg acgatatgtc tttgtaattc
      421 taatgatttt gataaccata tccatacttt ttaagtttgg aacaacacac acacaaaaca
      481 gtgcatttat ttcaataggc tcagttttta ctactgtttt agtcatttta gattcttatg
      541 tttttggaat ttgggtgttg cgattttcca aatacaacga attgtatggt tctatcggga
      601 ctttgttgat tatgatgttt tacatttgga taaactgcgc cattctgctt cttggtttcg
      661 aattaaatgc ttccataaat aaattgaaac aaaaaaagta acgaaaatta atactgaaga
      721 aaagaattaa ttttttcaaa attaaaatcc catataaata atgcattttg tagaagtaat
      781 tttaccgctt tcccttgaca aaacgtttac ttacagcgtt tctgaaaccg agtacaatta
      841 cattaaaata ggaatgcgtt tggctgtgcc tttcggcaaa agtaggatct acacggcttt
      901 ggttatagct attcaccaaa acaaacctac tttgtatgac gccaaggaaa ttcatcaaat
      961 tctggacgaa aaacccattg taaccgaaat tcagattact cattggcaat ggatagcatc
     1021 ttattatatg tgtgccattg gcgatgtgta tcgtggtgca atgccctcgg cacttttgct
     1081 ggaaagcgaa accattattt ctcaaaaaaa cgatggtttt gtagatgaaa gtttgctcac
     1141 agatgatgaa tttttaattt atgaagcctt gcagcaacaa agttctttga aagttcagga
     1201 tataatgagc attttgaata agaagaatat ttttccagtg attcaaaaat tgatagacaa
     1261 aaatattctg gtgcttcaag aagaaatgtt ggaaacttat acgccaaaac tcattcgata
     1321 cgtaaaattg cattccaaat acgaaacgaa tgaaggtttg agtgaattat tagaagtact
     1381 aaaaaatgcc aataagcaaa aggaaatcgt cttgagttat tttcaattga gtgcttccga
     1441 gaaaaaacca atcacagtca aaaagttaat tgaagttgca aattcgacat caacaattgt
     1501 aaaaacatta atcgataaag aaattttcga agaatattac attcaagtgg atcgtgttaa
     1561 tttcattgga aaaactaaag acgaacaact tcaattaagt gaagttcaac aaattgcttt
     1621 cgaagaaata aaagaaaatt ttacccaaaa agaagtctgt cttttgcacg gagt
//
LOCUS       DAOSNN010000332         1606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Flavobacterium alvei isolate AMR_MDS_2370
            NODE_76849_length_1606_cov_4.173436, whole genome shotgun sequence.
ACCESSION   DAOSNN010000332 DAOSNN010000000
VERSION     DAOSNN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864437
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flavobacterium alvei (wastewater metagenome)
  ORGANISM  Flavobacterium alvei
            Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
            Flavobacteriaceae; Flavobacterium.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:51:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,401
            CDSs (total)                      :: 2,379
            Genes (coding)                    :: 2,359
            CDSs (with protein)               :: 2,359
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 8 of 20
            Pseudo Genes (internal stop)      :: 6 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Flavobacterium alvei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76849_length_1606_cov_4.173436"
                     /isolate="AMR_MDS_2370"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2080416"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLQ70_12000"
     CDS             complement(<1..668)
                     /locus_tag="PLQ70_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017495620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HQE35384.1"
                     /translation="MKTIQIVATLLVLGVNTCLAQTNKSSSKDSKTYAEISVKEGGKW
                     EGNKYIGGTFKNIQSLKIAPKQTDHLEIIRYEGPGWESNKIAYRLYLDWRNAIDIFGK
                     KTDAVILPLVGQVGSDSYHNMQDWGCDILNSGKGIGIGSINRYLNNEKLHFREVDSTL
                     AKVENKVSESTVTINYYGWKTASDKIDFTSKLSIIPDQRYTKHTIQASKEIKGICTGI
                     VKQK"
     gene            complement(679..1605)
                     /locus_tag="PLQ70_12005"
     CDS             complement(679..1605)
                     /locus_tag="PLQ70_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQE35385.1"
                     /translation="MLFLFLLFTKVAFAQSHFPVDTTYTVKSVYDKVKKDHPYISYVP
                     TRSYETVNQKKEIVYKKIKDRELHLDAYYNKTNKQNPAIVIIHGGGWRSGNKSQMETF
                     AIEMASKGYSCFNIEFRLSMEAPYPAAIFDVKNAIQYIKKNARKFNVDTTKVAVLGCS
                     SGGQMASLIGTTNGKKEFEDSKTVSKFSSKVNAIVDVDGTLAFKHRESVEGKVAGLWL
                     GGSYEEIPSIWEQAAPLNQADKNTPPILFICSSIPRFHAGRDDMIAILNKYGIYSEIQ
                     TIPNSPHSFWFLNPWFDETIKYTTHFLDKIFK"
BASE COUNT          451 a          281 c          283 g          591 t
ORIGIN      
        1 tttttttgtt ttacaattcc agtacaaatt cctttgattt cttttgaagc ttgaatggtg
       61 tgttttgtat agcgctggtc tggaataatg gataacttgg atgtaaaatc aattttatcg
      121 gaagccgttt tccaaccata ataatttatt gtaacagtag attcgctgac tttattttcg
      181 actttagcaa gggtagaatc aacttcacgg aaatgtaatt tttcattgtt caaataccga
      241 tttatagaac caattccgat tccttttcct gaattcaaaa tatcgcatcc ccaatcttgc
      301 atattatgat aggaatcaga acctacctga cctactaatg gtaaaatcac ggcatcggtt
      361 ttttttccga aaatatcaat cgcattcctc caatctaagt acaaacgata ggctattttg
      421 ttgctttccc atccaggccc ttcatatcga ataatttcta aatgatcggt ttgttttgga
      481 gctattttta ggctttgaat gtttttaaaa gttcccccaa tatatttatt gccttcccat
      541 tttccgcctt ctttcaccga aatttcagca taggtttttg aatctttact tgaagattta
      601 ttggtttggg caaggcaggt attaacgcct aatactagta aagttgcgac tatttgtatt
      661 gttttcattt tataaatttc atttgaatat tttatctaaa aaatgagtcg tgtactttat
      721 agtttcatca aaccaagggt ttaggaacca aaaggagtgt ggcgaatttg gaatggtttg
      781 gatttcgctg tatattccat atttattgag tattgcaatc atatcgtctc ttccagcgtg
      841 aaatcttggg atactgctac aaatgaatag gataggaggt gtgtttttgt ctgcttgatt
      901 taatggagcc gcttgttccc aaatggaggg aatttcttca taacttccac ccaaccacaa
      961 gcctgccact ttgccttcta ccgattctct gtgcttgaat gccaatgtac catctacgtc
     1021 cacaattgca ttaacttttg aggaaaattt gctaacagtt ttggaatctt caaattcttt
     1081 tttaccattt gtagttccta tcagagaagc catttgtcca ccagaagagc aacctaaaac
     1141 ggccacttta gtggtgtcaa cattaaattt tcgtgcattt tttttgatgt actgaatggc
     1201 atttttgaca tcaaatattg cagcaggata aggtgcttcc attgataaac gaaattcaat
     1261 attaaaacaa gagtaccctt ttgaagccat ttctatggca aaagtttcca tctgagattt
     1321 attgccagat ctccaaccac caccgtgaat gataacaatt gcagggtttt gtttgttggt
     1381 tttattgtaa taagcatcaa gatgaagttc tctatctttt atttttttat aaacgatttc
     1441 ttttttctgg tttacggttt catagcttcg agttggtacg tagctaatgt agggatggtc
     1501 ttttttgact ttgtcgtaaa cactttttac ggtataagtg gtatcaacag gaaagtggct
     1561 ttgtgcaaaa gcaactttgg taaagagtaa aaaaaggaat agtatt
//
